title: Comparison of basophil activation tests using CD63 or CD203c expression in patients with insect venom allergy.
abstract: BACKGROUND
  Flow cytometric basophil activation tests have been developed as cellular tests for in vitro diagnosis of IgE-mediated reactions. Different activation markers (CD63 or CD203c) with distinct ways of regulation have been used after stimulation with various allergens.
  
  
  OBJECTIVE
  It was the aim of the present study to compare basophil activation tests by measuring both CD63 and CD203c upregulation in patients with insect venom allergy.
  
  
  METHODS
  43 patients with a history of insect venom anaphylaxis were examined. A careful allergy history was taken, and skin tests and determination of specific IgE-antibodies were performed. Basophil activation tests (BAT) using CD63 or CD203c expression were done after stimulation with different concentrations of bee and wasp venom extracts. 25 healthy subjects with negative history of insect venom allergy were studied as controls.
  
  
  RESULTS
  The CD203c protocol showed a slightly higher sensitivity than the CD63 protocol (97% vs. 89%) with regard to patients' history. The magnitude of basophil response was higher with CD203c in comparison to CD63 for both insect venoms. Specificity was 100% for the CD63 protocol and 89% for the CD203c protocol with regard to controls with negative history and negative RAST.
  
  
  CONCLUSIONS
  These results support the reliability of basophil activation tests using either CD63 or CD203c as cellular tests in the in vitro diagnosis of patients with bee or wasp venom allergy with a slightly higher sensitivity for the CD203c protocol.
authors.affiliation: Department of Dermatology and Allergy Biederstein, Technical University Munich, Munich, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Eberlein-König
authors.forename: R
authors.initials: R
authors.lastname: Varga
authors.forename: M
authors.initials: M
authors.lastname: Mempel
authors.forename: U
authors.initials: U
authors.lastname: Darsow
authors.forename: H
authors.initials: H
authors.lastname: Behrendt
authors.forename: J
authors.initials: J
authors.lastname: Ring
mesh_terms: D000485:Allergens / Q000276:immunology*; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics; D015549:Basophil Degranulation Test; D001514:Bee Venoms / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D007299:Insect Bites and Stings / Q000276:immunology*; D010727:Phosphoric Diester Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D011755:Pyrophosphatases / Q000096:biosynthesis* / Q000235:genetics; D012680:Sensitivity and Specificity; D060149:Tetraspanin 30; D014862:Wasp Venoms / Q000276:immunology*
chemical_list: D000485:Allergens; D015703:Antigens, CD; D001514:Bee Venoms; C554586:CD63 protein, human; C427944:ENPP3 protein, human; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; D014862:Wasp Venoms; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases