Numerical simulation of microcarrier motion in a rotating wall vessel bioreactor.
OBJECTIVE
To analyze the forces of rotational wall vessel (RWV) bioreactor on small tissue pieces or microcarrier particles and to determine the tracks of microcarrier particles in RWV bioreactor.


METHODS
The motion of the microcarrier in the rotating wall vessel (RWV) bioreactor with both the inner and outer cylinders rotating was modeled by numerical simulation.


RESULTS
The continuous trajectory of microcarrier particles, including the possible collision with the wall was obtained. An expression between the minimum rotational speed difference of the inner and outer cylinders and the microcarrier particle or aggregate radius could avoid collisions with either wall. The range of microcarrier radius or tissue size, which could be safely cultured in the RWV bioreactor, in terms of shear stress level, was determined.


CONCLUSIONS
The model works well in describing the trajectory of a heavier microcarrier particle in rotating wall vessel.
Ju
Zhi-Hao
ZH
State Key Laboratory of Fine Chemical Engineering, Stem Cells & Tissue Engineering Laboratory, Dalian University of Technology, Liaoning, China.
Liu
Tian-Qing
TQ
Ma
Xue-Hu
XH
Cui
Zhan-Feng
ZF
D019149:Bioreactors; D003198:Computer Simulation; D008863:Microspheres; D009038:Motion; D016062:Porosity; D012212:Rheology; D012399:Rotation; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*