Introduction to the polymerase chain reaction.
The polymerase chain reaction (PCR) is an in vitro method for the amplification of DNA. Since the introduction of the PCR in 1985, it has become an indispensable technique for many applications in scientific research and clinical and forensic investigations. In this chapter, the principle and setup of PCR, as well as the methods for analyzing PCR products, will be discussed.
Lo
Y M Dennis
YM
Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR.
Chan
K C Allen
KC
D004247:DNA / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D008957:Models, Genetic; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004247:DNA