title: [Relationship of heterogeneous sensitivity to metal ion and its cell cycle in Scenedesmus obliquus].
abstract: It is important to investigate the effect of contamination on the heterogeneous cells characteristically observed within single algae species, which can be helpful to understand the sensitivity of organism and the toxicity mechanism. Flow cytometry was used to assess sensitivity to metal ion (Cd2+ and Ni2+) in Scenedesmus obliquus. By determination of esterase activity in cell fractions gated by FSC (an indicator of the cell volume), it was shown that the resistance to metal ion was improved as the cell volume increased. By simultaneously examining FSC and DNA content, a relationship between the cell volume and the cell cycle stage was observed: as the cell volume increased, the S. obliquus cells changed from G1 phase to G2/M phase. The cells in G2/M phase had more resistance to metal ion. Thus, this observation indicate that the difference of cell cycle may be the important cause of heterogeneous sensitivity to metal ion in S. obliquus.
authors.affiliation: Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, China. nju-yuyang@yahoo.com
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Fan-xiang
authors.initials: FX
authors.lastname: Kong
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Shi
mesh_terms: D002104:Cadmium / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects*; D005434:Flow Cytometry; D009532:Nickel / Q000633:toxicity*; D044444:Scenedesmus / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D018675:Toxicity Tests / Q000379:methods; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D002104:Cadmium; D009532:Nickel