Cells of cutaneous immunity in Xenopus: studies during larval development and limb regeneration.
The anuran Xenopus laevis is an experimental model for vertebrate development, immunology, and regenerative biology. Using histochemistry and immunohistochemistry (IHC) we examined embryonic, larval, and postmetamorphic Xenopus skin for the presence of dendritic cells (DCs), Langerhans cells (LCs), and dendritic epidermal T cells (DETCs), all components of cutaneous immunity that have been implicated in skin repair and regeneration. Cells expressing three markers for dendritic and Langerhans cells (formalin-resistant ATPase activity, major histocompatibility complex [MHC] class II antigens, and vimentin) and having morphology like that of these cells first appeared during late embryonic stages, becoming abundant by prometamorphosis. Cells positive for these markers were also numerous in the wound epithelia of regenerating hindlimbs at both early and late larval stages. Cells tentatively identified as DETCs were found, beginning at early larval stages, using IHC with antibodies against heterologous CD3epsilon chain and T-cell receptor delta. Further characterization and work with the putative DCs, LCs, and DETCs demonstrated here will allow not only greater understanding of the amphibian immune system, but also further elucidation of regenerative growth and scarring.
Mescher
Anthony L
AL
Department of Anatomy and Cell Biology, Indiana University School of Medicine, Medical Sciences, Center for Regenerative Biology and Medicine, Jordan Hall, Bloomington, IN 47405, USA. mescher@indiana.edu
Wolf
Wendy L
WL
Moseman
E Ashley
EA
Hartman
Byron
B
Harrison
Clint
C
Nguyen
Evelyn
E
Neff
Anton W
AW
D000818:Animals; D003713:Dendritic Cells / Q000276:immunology; D005121:Extremities / Q000502:physiology*; D007801:Langerhans Cells / Q000276:immunology; D007814:Larva / Q000166:cytology / Q000254:growth & development / Q000276:immunology; D012038:Regeneration / Q000276:immunology*; D012867:Skin / Q000166:cytology* / Q000254:growth & development / Q000276:immunology*; D014982:Xenopus laevis / Q000276:immunology* / Q000502:physiology