title: Controlled release of DNA/polyamine complex by photoirradiation of a solid phase presenting o-nitrobenzyl ether tethered spermine or polyethyleneimine.
abstract: Various gene transfer and automated/monitorized analytical applications require the controlled release of nucleic acid. A solid phase with spermine or polyethyleneimines (PEI, 600 MW) tethered by o-nitrobenzyl linkages was synthesized with polyethylene oxide beads (ArgoGel-NH(2)). The photolysis of test compound O-2-nitrophenethyl O,O-diethyl phosphate or solid phase with o-nitrobenzyl group as synthetic linker was completely degradable with photoirradiation at 365 nm for 10-18 min at 3.5 mW/cm(2). DNA binding with polyamine of the solid phase and releasing of DNA/polyamine were monitored by UV measurement and gel electrophoresis. The potential exists to employ a DNA-loaded solid phase for spatially, temporally, or dose-controlled release of DNA, at extracellular or intracellular sites.
authors.affiliation: Institute for Medicine and Engineering, Department of Chemical and Biomolecular Engineering, 1024 Vagelos Research Laboratory, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Moon Suk
authors.initials: MS
authors.lastname: Kim
authors.forename: Scott L
authors.initials: SL
authors.lastname: Diamond
mesh_terms: D004247:DNA / Q000008:administration & dosage / Q000378:metabolism*; D016503:Drug Delivery Systems; D004987:Ethers; D006801:Humans; D008027:Light; D011073:Polyamines / Q000008:administration & dosage / Q000378:metabolism*; D011094:Polyethyleneimine; D013096:Spermine
chemical_list: D004987:Ethers; D011073:Polyamines; D013096:Spermine; D011094:Polyethyleneimine; D004247:DNA