[Inhibited proliferation of B-lymphoma Raji cells and down-regulated expression of VEGF by arsenic trioxide].
This study was aimed to investigate the effects of As2O3 on proliferation of B lymphoma Raji cell and to study the expression changes of VEGF mRNA. The modified MTT was adopted to evaluate the effect of As2O3 on proliferation of Raji cells. Semi-quantitative RT-PCR was used to detect the expression of VEGF121 and VEGF165 mRNA in Raji cells exposed to As2O3. The results showed that the As2O3 significantly inhibited the proliferation of Raji cells, the relationship between the inhibition rate and the concentrations of As2O3 was dose- and time-dependent. The VEGF121 and VEGF165 mRNA expressions were found in Raji cells, and both were well-matched in expression levels. After treatment of As2O3 at 1 micromol/L for 48 hours, the expression levels of VEGF121 and VEGF165 mRNA were significantly down-regulated and demonstrated negative correlation with the time of exposure to As2O3. Otherwise some difference were observed in effects of As2O3 on VEGF121 and VEGF165, the expression of VEGF165 mRNA was down-regulated earlier and longer than that of VEGF121, while no similar correlation was found in selected concentration groups. It is concluded that As2O3 can significantly inhibit the growth of Raji cells and may exerted its anti-angiogenesis effects by down-regulating the VEGF mRNA expression even in a low concentration for a long term.
Yu
Mei-Jia
MJ
Department of Hematology, The First Affiliated Hospital of Kunming Medical College, Kunming, 650031, China.
Duan
Yong
Y
Liu
Hua
H
Zhang
Xue-Mei
XM
Li
Hui-Min
HM
D000970:Antineoplastic Agents / Q000494:pharmacology; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D015536:Down-Regulation; D006801:Humans; D016393:Lymphoma, B-Cell / Q000378:metabolism / Q000473:pathology*; D010087:Oxides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D000077237:Arsenic Trioxide