Arabidopsis tubulin folding cofactor B interacts with alpha-tubulin in vivo.
Microtubule biogenesis requires alphabeta tubulin dimers that are generated from alpha and beta tubulin following post-translational modification by several tubulin folding cofactors (TFCs). Here we report the isolation and characterization of Arabidopsis TFCB (AtTFCB). AtTFCB is expressed in all organs of Arabidopsis. The subcellular localization of AtTFCB is mainly cytosolic. AtTFCB-overexpressing cells have fewer microtubules compared with the controls. Multimode fluorescence resonance energy transfer (FRET) microscopy reveals a direct physical interaction of AtTFCB with alpha tubulin in living plant cells. We conclude that AtTFCB interacts with alpha tubulin in vivo and its overexpression reduces the number of microtubules.
Dhonukshe
Pankaj
P
Section of Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands. pankaj.dhonukshe@zmbp.uni-tuebingen.de
Bargmann
Bastiaan O R
BO
Gadella
Theodorus W J
TW
D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D018506:Gene Expression Regulation, Plant; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D022101:Microtubule-Organizing Center / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D014404:Tubulin / Q000378:metabolism*
D029681:Arabidopsis Proteins; D008869:Microtubule-Associated Proteins; D014404:Tubulin