Theoretical model of the three-dimensional structure of a disease resistance gene homolog encoding resistance protein in Vigna mungo.
Plant disease resistance (R) genes, the key players of innate immunity system in plants encode 'R' proteins. 'R' protein recognizes product of avirulance gene from the pathogen and activate downstream signaling responses leading to disease resistance. No three dimensional (3D) structural information of any 'R' proteins is available as yet. We have reported a 'R' gene homolog, the 'VMYR1', encoding 'R' protein in Vigna mungo. Here, we describe the homology modeling of the 'VMYR1' protein. The model was created by using the 3D structure of an ATP-binding cassette transporter protein from Vibrio cholerae as a template. The strategy for homology modeling was based on the high structural conservation in the superfamily of P-loop containing nucleoside triphosphate hydrolase in which target and template proteins belong. This is the first report of theoretical model structure of any 'R' proteins.
Basak
Jolly
J
Plant Molecular and Cellular Genetics Section, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata 700054. India. jolly.basak@ese.u-psud.fr
Bahadur
Ranjit P
RP
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D019281:Dimerization; D007887:Fabaceae / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017385:Sequence Homology; D040681:Structural Homology, Protein; D014734:Vibrio cholerae / Q000737:chemistry / Q000235:genetics
D018528:ATP-Binding Cassette Transporters