Glucocorticoid regulation of 24-hour oscillation in interferon receptor gene expression in mouse liver.
Although the antiviral effect of interferon (IFN) varies depending on 24-h oscillation in the expression of its specific receptor, the mechanism of oscillation remains to be clarified. Here we report that oscillation in the expression of the IFN receptor gene (IFN-alpha/beta R1) in mouse liver is caused by the endogenous rhythm of glucocorticoid secretion. Brief exposure of mouse hepatic cells (Hepa 1-6) to corticosterone (CORT) resulted in a significant decrease in mRNA levels of IFN-alpha/beta R1. The CORT-induced decrease in IFN-alpha/beta R1 mRNA levels was reversed by pretreating the cells with RU486, a glucocorticoid receptor antagonist. The mRNA levels of IFN-alpha/beta R1 gene in the liver of adrenalectomized mice were consistently increased throughout the day. However, a single administration of CORT to adrenalectomized mice significantly decreased the mRNA levels of IFN-alpha/beta R1 in the liver. Furthermore, the rhythmic phase of IFN-alpha/beta R1 expression was modulated after the alteration of rhythmicity in glucocorticoid secretion, which was induced by restricted daily feeding. As a consequence, under manipulation of the feeding schedule, 2'-5' oligoadenylate synthase activities, as an index of antiviral effect, in plasma and liver at 24 h after IFN-alpha injection also varied depending on the alteration of glucocorticoid secretion rhythm. These results suggest that the endogenous rhythm of glucocorticoid secretion is involved in the circadian regulation of IFN-alpha/beta R1 expression in mouse liver. Our findings also support the notion that monitoring the 24-h variation in IFN receptor function is useful for selecting the most appropriate time of day to administer IFN.
Koyanagi
Satoru
S
Pharmaceutics, Department of Medico-Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan.
Suyama
Hinako
H
Kuramoto
Yukako
Y
Matsunaga
Noaya
N
Takane
Hiroshi
H
Soeda
Shinji
S
Shimeno
Hiroshi
H
Higuchi
Shun
S
Ohdo
Shigehiro
S
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000315:Adrenalectomy; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002478:Cells, Cultured; D002940:Circadian Rhythm; D003345:Corticosterone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D016898:Interferon-alpha / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D053633:Receptor, Interferon alpha-beta / Q000235:genetics*
D000998:Antiviral Agents; D016898:Interferon-alpha; D012333:RNA, Messenger; D053633:Receptor, Interferon alpha-beta; D015088:2',5'-Oligoadenylate Synthetase; D003345:Corticosterone