Kinetic flux profiling of nitrogen assimilation in Escherichia coli.
We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.
Yuan
Jie
J
Department of Chemistry and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA.
Fowler
William U
WU
Kimball
Elizabeth
E
Lu
Wenyun
W
Rabinowitz
Joshua D
JD
D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D009584:Nitrogen / Q000378:metabolism*; D013997:Time Factors
D009584:Nitrogen