title: A third-generation H2O2 biosensor based on horseradish peroxidase-labeled Au nanoparticles self-assembled to hollow porous polymeric nanopheres.
abstract: A novel third-generation biosensor for hydrogen peroxide (H2O2) was developed by self-assembling gold nanoparticles to hollow porous thiol-functionalized poly(divinylbenzene-co-acrylic acid) (DVB-co-AA) nanospheres. At first, a cleaned gold electrode was immersed in hollow porous thiol-functionalized poly(DVB-co-AA) nanosphere latex to assemble the nanospheres, then gold nanoparticles were chemisorbed onto the thiol groups of the nanospheres. Finally, horseradish peroxidase (HRP) was immobilized on the surface of the gold nanoparticles. The immobilized horseradish peroxidase exhibited direct electrochemical behavior toward the reduction of hydrogen peroxide. The resulting biosensor showed a wide linear range of 1.0 microM-8.0mM and a detection limit of 0.5 microM estimated at a signal-to-noise ratio of 3. Moreover, the studied biosensor exhibited high sensitivity, good reproducibility, and long-term stability.
authors.affiliation: Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 Jilin, PR China. syxu0212@yahoo.com
authors.forename: Shiyi
authors.initials: S
authors.lastname: Xu
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.forename: Xiaozu
authors.initials: X
authors.lastname: Han
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis*; D053758:Nanoparticles; D043942:Nanotubes
chemical_list: D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase