title: Human alveolar bone cells interact with ProRoot and tooth-colored MTA.
abstract: The cellular response to mineral trioxide aggregate (MTA) is important for the repair and regeneration of periradicular tissues. The purpose of this study was to analyze the response of human alveolar bone cells to MTA. A human alveolar bone chip was obtained from an oral surgical procedure and explant cultures harvested after 3 to 4 weeks of outgrowth in alpha-minimum essential medium supplemented with fetal calf serum. Cells in early passage were seeded onto preset ProRoot (gray) MTA, tooth-colored (white) MTA, and MTA prepared with local anesthetic solution. Scanning electron microscopy showed cells were attached and spread out onto MTA within 24 hours, and proliferated to form a matrix-like layer within 7 days. Cell attachment and cell-surface interactions with the gray and white MTA, and with the MTA prepared with local anesthetic were comparably propagated for 14 days. The surgically derived human alveolar bone cells provided a clinically relevant model that demonstrated the capacity of both ProRoot and tooth-colored MTA to support cell attachment, proliferation, and matrix formation.
authors.affiliation: Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, NY 14214-8031, USA.
authors.forename: Ebtehal
authors.initials: E
authors.lastname: Al-Rabeah
authors.forename: Hiran
authors.initials: H
authors.lastname: Perinpanayagam
authors.forename: Don
authors.initials: D
authors.lastname: MacFarland
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000539:Alveolar Process / Q000166:cytology / Q000187:drug effects*; D017610:Calcium Compounds / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004338:Drug Combinations; D005109:Extracellular Matrix; D006801:Humans; D008422:Materials Testing; D046529:Microscopy, Electron, Transmission; D010006:Osteoblasts / Q000187:drug effects*; D010087:Oxides / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate