title: Dietary fatty acid composition rather than vitamin E supplementation influence ex vivo cytokine and eicosanoid response of porcine alveolar macrophages.
abstract: This study examined the influence of different dietary fat sources (animal fat, sunflower oil, and fish oil) and supplementation of vitamin E (85, 150 and 300 mg all-rac-alpha-tocopheryl acetate/kg diet) on the ex vivo synthesis of eicosanoids and cytokines by porcine alveolar macrophages. Supplementation of vitamin E provoked an increase in the concentration of alpha-tocopherol of the macrophages irrespective of fat sources. Fish oil increased the macrophage n-3 content with 100% and 40%, and reduced the n-6 with 60% and 53% in comparison with sunflower oil and animal fat, respectively. Fish oil decreased the production of TNF-alpha, IL-8, LTB4, and PGE2 (but not IL-6) relative to the other dietary fat sources, and no difference was observed between sunflower oil and animal fat. Positive correlations were found between the n-6 fatty acid content and the production of PGE2, and the PGE2 production was positively correlated with TNF-alpha and IL-8. Negative correlations were found between the n-3 PUFA content and the concentration of PGE2, TNF-alpha and IL-8. In conclusion, dietary fish oil supplemented at a level of 5%, but not supplemental vitamin E, influenced the inflammatory responses of alveolar macrophages isolated from weaned pigs relatively to animal fat and sunflower oil.
authors.affiliation: Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark.
authors.forename: S
authors.initials: S
authors.lastname: Møller
authors.forename: C
authors.initials: C
authors.lastname: Lauridsen
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism* / Q000494:pharmacology; D019587:Dietary Supplements / Q000032:analysis; D015777:Eicosanoids / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism* / Q000502:physiology; D005260:Female; D016676:Macrophages, Alveolar / Q000378:metabolism*; D013552:Swine; D014810:Vitamin E / Q000008:administration & dosage* / Q000502:physiology
chemical_list: D016207:Cytokines; D004041:Dietary Fats; D015777:Eicosanoids; D005227:Fatty Acids; D014810:Vitamin E