title: Identification of a novel glycosyltransferase involved in LOS biosynthesis of Moraxella catarrhalis.
abstract: Moraxella catarrhalis is an important human mucosal pathogen that contributes to otitis media in infants and exacerbates conditions such as chronic obstructive pulmonary disease in the elderly. This study describes the identification of a novel gene, lgt5 that encodes a glycosyltransferase involved in the LOS biosynthesis of M. catarrhalis. Analysis of NMR data of LOS-derived oligosaccharide from a Serotype A lgt5 mutant strain of M. catarrhalis indicate that lgt5 encodes an alpha-(1-->4)-galactosyltransferase.
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast, 4215 QLD, Australia.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Wilson
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Collins
authors.forename: Zoran
authors.initials: Z
authors.lastname: Klipic
authors.forename: I Darren
authors.initials: ID
authors.lastname: Grice
authors.forename: Ian R
authors.initials: IR
authors.lastname: Peak
mesh_terms: D000942:Antigens, Bacterial / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D017931:DNA Primers; D005838:Genotype; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D001936:Moraxella catarrhalis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; C023023:lipid-linked oligosaccharides; D005964:Glucosyltransferases; C114421:lipooligosaccharide beta chain synthesizing glucosyltransferase