Cell death-inducing DFF45-like effector may take part in neuronal apoptosis of the lumbar spinal cord after sciatic nerve injury caused by a firearm.
OBJECTIVE
To investigate neuronal apoptosis and gene regulation after peripheral nerve injury caused by a firearm.


METHODS
A rabbit model of sciatic nerve injury caused by a firearm was used. Neuronal apoptosis was determined by using terminal transferase-mediated dUTP nick end-labeling and flow cytometry. Differentially expressed genes were screened with mouse_8192S GeneChips. Cell death-inducing DFF45-like effector (CIDE-B) gene expression in spinal cord after sciatic nerve injury was detected with reverse transcription-polymerase chain reaction.


RESULTS
The size of the injury caused by the firearm was widely extended. Neuronal apoptosis was found in days 1, 3, and 7 groups. The expression of apoptosis-related gene CIDE-B was high according to analysis of the GeneChips and reverse transcription-polymerase chain reaction detection. The trend of CIDE-B expression was consistent with neuronal apoptosis after nerve injury.


CONCLUSIONS
Neuronal apoptosis is an important pathological change after peripheral nerve injury caused by firearms, and CIDE-B may be an important regulator.
Wang
Jian-Min
JM
Institute of Surgery Research, Daping Hospital, The Third Military Medical University, Chongqing 400042, People's Republic of China.
Li
Bing-Cang
BC
Chen
Lei
L
Wang
Gui-Bo
GB
Sun
Hongzheng
H
Chen
Zhiqiang
Z
Zhang
Liang-Chao
LC
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D020287:In Situ Nick-End Labeling; D008159:Lumbar Vertebrae / Q000293:injuries*; D008297:Male; D023421:Models, Animal; D012313:RNA / Q000032:analysis; D011817:Rabbits; D012584:Sciatic Nerve / Q000293:injuries*; D013119:Spinal Cord Injuries / Q000503:physiopathology; D019098:Telomerase; D014948:Wounds, Gunshot / Q000503:physiopathology*
D012313:RNA; D019098:Telomerase