Identification of novel foot-and-mouth disease virus specific T-cell epitopes in c/c and d/d haplotype miniature swine.
To identify foot-and-mouth disease virus (FMDV) specific T-cell epitopes within the non-structural protein 3D in swine, pentadecapeptides were tested in proliferation and Interferon-gamma ELISPOT assays using lymphocytes from two strains of inbred miniature pigs (c/c and d/d haplotype) experimentally infected with FMDV. Lymphocytes of c/c pigs recognized peptides from three different regions in 3D, d/d lymphocytes recognized peptides from two regions, one of them being adjacent to an epitope of c/c pigs and comprising amino acid residues 346-370. Analyses of the response of d/d lymphocytes against peptides representing the structural protein 1A revealed another novel T-cell epitope. Investigation of the phenotype of responding lymphocytes showed a response of CD4(+)CD8(+)MHC-class-II(+) cells, identifying them as activated T-helper cells. This is the first report on FMDV specific T-cell epitopes recognized by swine leukocyte antigen (SLA) inbred swine and provides information useful for the design of novel vaccines against FMDV.
Gerner
Wilhelm
W
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Surrey, UK. Wilhelm.Gerner@vu-wien.ac.at
Denyer
Michael S
MS
Takamatsu
Haru-Hisa
HH
Wileman
Thomas E
TE
Wiesmüller
Karl-Heinz
KH
Pfaff
Eberhard
E
Saalmüller
Armin
A
D000595:Amino Acid Sequence; D000818:Animals; D017849:Animals, Inbred Strains; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005536:Foot-and-Mouth Disease / Q000276:immunology*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D006239:Haplotypes; D006649:Histocompatibility Antigens / Q000276:immunology; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000276:immunology; D013552:Swine; D013556:Swine, Miniature; D037182:T-Cell Antigen Receptor Specificity; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000138:chemical synthesis / Q000276:immunology
D018984:Epitopes, T-Lymphocyte; D006649:Histocompatibility Antigens; D010455:Peptides; D017361:Viral Nonstructural Proteins; D007371:Interferon-gamma