title: Alpha-lipoic acid suppresses the development of collagen-induced arthritis and protects against bone destruction in mice.
abstract: OBJECTIVE
  To test the ability of alpha-lipoic acid (LA) to attenuate the development of collagen-induced arthritis (CIA) in mice.
  
  
  METHODS
  Mice were divided into three groups and treated with intraperitoneal administration of LA (10 or 100 mg/kg) or placebo. Clinical, histologic, and biochemical parameters were assessed. Human synovial fibroblasts and peripheral blood mononuclear cells were cocultured in various concentrations of LA to evaluate the effects on osteoclastogenesis.
  
  
  RESULTS
  LA was associated with a dose-dependent reduction of CIA, as well as preventing bone erosion and destructive changes. Intracellular reactive oxygen species in lymphocytes obtained from inguinal lymph nodes, which was significantly higher in CIA than control mice, was significantly reduced in CIA by LA. The concentrations of TNF-alpha, IL-1beta, and IL-6 in the paws, and synovial NF-kappaB binding, all of which were markedly higher in CIA than control mice, were reduced by treatment with LA. In addition, LA inhibited the formation of human osteoclasts in vitro.
  
  
  CONCLUSIONS
  Amelioration of joint disease by LA was associated with reduction in oxidative stress, as well as inhibition of inflammatory cytokine activation and NF-kappaB DNA binding activity. Moreover, LA inhibited bone destruction in vivo and osteoclastogenesis in vitro. Collectively, these results indicate that LA may be a new adjunctive therapy for rheumatoid arthritis.
authors.affiliation: Division of Allergy and Rheumatology, Department of Internal Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, 138-736, Republic of Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Lee
authors.forename: Chang-Keun
authors.initials: CK
authors.lastname: Lee
authors.forename: Ki-Up
authors.initials: KU
authors.lastname: Lee
authors.forename: Joong Yeol
authors.initials: JY
authors.lastname: Park
authors.forename: Kyung-Ja
authors.initials: KJ
authors.lastname: Cho
authors.forename: You Sook
authors.initials: YS
authors.lastname: Cho
authors.forename: Hee Ran
authors.initials: HR
authors.lastname: Lee
authors.forename: Se Hwan
authors.initials: SH
authors.lastname: Moon
authors.forename: Hee-Bom
authors.initials: HB
authors.lastname: Moon
authors.forename: Bin
authors.initials: B
authors.lastname: Yoo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000188:drug therapy*; D016207:Cytokines; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000187:drug effects; D010010:Osteoclasts / Q000187:drug effects; D011897:Random Allocation; D013582:Synovial Fluid / Q000187:drug effects / Q000276:immunology; D008063:Thioctic Acid / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D016207:Cytokines; D016328:NF-kappa B; D008063:Thioctic Acid