Ionic basis of a mechanotransduction current in adult rat dorsal root ganglion neurons.
Sensory mechanical transduction - necessary for hearing, proprioception, and the senses of touch and pain - remains poorly understood. In somatosensation, even the basic properties of the mechanically sensitive excitatory ionic currents that are assumed to mediate mechanical transduction are largely undescribed. We have recorded, from the soma of rat dorsal root ganglion (DRG) neurons in vitro, whole-cell ionic currents induced by the impact of a piezo-electrically driven glass probe. This transient mechanically activated current was observed in virtually all DRG neurons tested. In ion substitution experiments the current could be carried nonselectively by most cations, including divalent and organic cations, but not by chloride or sulfate ions. In addition, the mechanically activated current carried by monovalent cations was consistently blocked by millimolar concentrations of external calcium or magnesium. Based on these results, the transient mechanical transduction current observed in somatosensory neurons in vitro is mediated by large-pore mechanically gated channels nonselective for cations but impermeable to anions.
McCarter
Gordon C
GC
Department of Oral and Maxillofacial Surgery, Division of Neurosciences, University of California at San Francisco, San Francisco, CA 94143-0440, USA. gmccarter@touro.edu
Levine
Jon D
JD
D000818:Animals; D002413:Cations, Divalent / Q000378:metabolism; D002414:Cations, Monovalent / Q000378:metabolism; D002794:Choline / Q000493:pharmacokinetics; D004553:Electric Conductivity; D005727:Ganglia, Spinal / Q000502:physiology*; D005971:Glutamates / Q000493:pharmacokinetics; D007477:Ions / Q000378:metabolism; D008094:Lithium / Q000493:pharmacokinetics; D008274:Magnesium / Q000493:pharmacokinetics; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008962:Models, Theoretical; D009475:Neurons, Afferent / Q000502:physiology*; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000493:pharmacokinetics; D013379:Substrate Specificity; D019789:Tetraethylammonium / Q000493:pharmacokinetics; D014325:Tromethamine / Q000493:pharmacokinetics; D050994:Voltage-Dependent Anion Channels / Q000378:metabolism / Q000502:physiology*
D002413:Cations, Divalent; D002414:Cations, Monovalent; D005971:Glutamates; D007477:Ions; D050994:Voltage-Dependent Anion Channels; D014325:Tromethamine; C014574:gamma-glutamylmethylamide; D019789:Tetraethylammonium; D008094:Lithium; D012964:Sodium; D008274:Magnesium; D002794:Choline