title: Non-cell-autonomous rescue of anaphase-promoting complex function revealed by mosaic analysis of HOBBIT, an Arabidopsis CDC27 homolog.
abstract: The Arabidopsis HOBBIT (HBT) gene encodes a homolog of the CDC27 anaphase-promoting complex/cyclosome subunit and is essential for postembryonic development. We induced loss-of-function clones by Cre/lox-mediated recombination of a single complementing HBT transgene in a background homozygous for the strong mutant allele hbt(2311). Defects in cell division and cell expansion are the primary consequences of ubiquitous postembryonic HBT excision. In roots, both cell division and cell expansion are rapidly affected. In contrast, in leaf primordia, cell division and cell expansion halt after a lag phase, which results in different severities of defects in the proximodistal and mediolateral axes. Surprisingly, small clones reveal non-cell-autonomous rescue of hbt mutant cells, indicating a previously unrecognized compensation mechanism for reduced activity of an anaphase-promoting complex/cyclosome component critical for cell cycle progression.
authors.affiliation: Department of Molecular Genetics, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
authors.forename: Olivier
authors.initials: O
authors.lastname: Serralbo
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Pérez-Pérez
authors.forename: Renze
authors.initials: R
authors.lastname: Heidstra
authors.forename: Ben
authors.initials: B
authors.lastname: Scheres
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome; D017360:Arabidopsis / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D002999:Clone Cells; D006720:Homozygote; D009030:Mosaicism; D009154:Mutation / Q000235:genetics; D018515:Plant Leaves / Q000166:cytology; D018517:Plant Roots / Q000166:cytology; D017385:Sequence Homology; D043743:Ubiquitin-Protein Ligase Complexes / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; C467333:HOBBIT protein, Arabidopsis; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome