title: Development and genetic mapping of sequence-tagged microsatellites (STMs) in bread wheat (Triticum aestivum L.).
abstract: The density of SSRs on the published genetic map of bread wheat (Triticum aestivum L.) has steadily increased over the last few years. This has improved the efficiency of marker-assisted breeding and certain types of genetic research by providing more choice in the quality of SSRs and a greater chance of finding polymorphic markers in any cross for a chromosomal region of interest. Increased SSR density on the published wheat genetic map will further enhance breeding and research efforts. Here, sequence-tagged microsatellite profiling (STMP) is demonstrated as a rapid technique for the economical development of anonymous genomic SSRs to increase marker density on the wheat genetic map. A total of 684 polymorphic sequence-tagged microsatellites (STMs) were developed, and 380 were genetically mapped in three mapping populations, with 296 being mapped in the International Triticeae Mapping Initiative W7984 x Opata85 recombinant inbred cross. Across the three populations, a total of 479 STM loci were mapped. Several technological advantages of STMs over conventional SSRs were also observed. These include reduced marker deployment costs for fluorescent-based SSR analysis, and increased genotyping throughput by more efficient electrophoretic separation of STMs and a high amenability to multiplex PCR.
authors.affiliation: Plant Functional Genomics Center, University of Adelaide, Hartley Grove, Urrbrae, SA, 5064, Australia. matthew.hayden@adelaide.edu.au
authors.forename: M J
authors.initials: MJ
authors.lastname: Hayden
authors.forename: P
authors.initials: P
authors.lastname: Stephenson
authors.forename: A M
authors.initials: AM
authors.lastname: Logojan
authors.forename: D
authors.initials: D
authors.lastname: Khatkar
authors.forename: C
authors.initials: C
authors.lastname: Rogers
authors.forename: J
authors.initials: J
authors.lastname: Elsden
authors.forename: R M D
authors.initials: RM
authors.lastname: Koebner
authors.forename: J W
authors.initials: JW
authors.lastname: Snape
authors.forename: P J
authors.initials: PJ
authors.lastname: Sharp
mesh_terms: D002874:Chromosome Mapping; D017931:DNA Primers; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D016324:Sequence Tagged Sites; D014908:Triticum / Q000235:genetics*
chemical_list: D017931:DNA Primers