[Quantitative determination of the (E) - and (Z) -diastereomers of resveratrol and resveratrol glucoside in the roots of Polygonum cuspidatum by HPLC and elementary study on their fluorescence].
OBJECTIVE
To establish an optimal HPLC method for the determination of the (E)- and (Z)-diastereomers of resveratrol and resveratrol glucoside from the roots of Polygonum cuspidatum.


METHODS
The determination was conducted by using reversed-phase high liquid chromatography. Nucleodur 100-5 C18 column (250 mm x 4.6 mm ID, 5 microm), and a mobile phase program of gradient elution with isopropyl alcohol and water at a flow rate of 0.6 mL x min(-1) was employed. The fluorescence detection wavelengths were: lambda(ex) 334 nm, lambda(em) 404 nm.


RESULTS
A good linear relationship was obtained under the optimum condition. The average recoveries were 96.7%, 99.1% for the (E)-diastereomers of resveratrol and resveratrol glucoside, 91.1%, 93.7% for the (Z)-diastereomers of resveratrol and resveratrol glucoside, respectively. The RSD of E-resveratrol and its glucoside were 1.34% and 0.72%, respectively. The RSD of Z-resveratrol and its glucoside were 1.27% and 2.08%, respectively.


CONCLUSIONS
This method is accurate and reliable for the quantity analysis of the (E)- and (Z)-diastereomers of resveratrol and resveratrol glucoside in the roots of Polygonum cuspidatum.
Wu
Bo
B
Hubei Key Laboratory for Processing and Transformation of Agricultural Product, Wuhan Polytechnic University, Wuhan 420023, China. wb@whpu.edu.cn
Zhang
Han-Jun
HJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D045468:Fallopia japonica / Q000737:chemistry*; D005453:Fluorescence; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D000077185:Resveratrol; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013267:Stilbenes / Q000032:analysis* / Q000737:chemistry
D005960:Glucosides; D013267:Stilbenes; D000077185:Resveratrol; C058229:polydatin