title: Coatomer, the coat protein of COPI transport vesicles, discriminates endoplasmic reticulum residents from p24 proteins.
abstract: In the formation of COPI vesicles, interactions take place between the coat protein coatomer and membrane proteins: either cargo proteins for retrieval to the endoplasmic reticulum (ER) or proteins that cycle between the ER and the Golgi. While the binding sites on coatomer for ER residents have been characterized, how cycling proteins bind to the COPI coat is still not clear. In order to understand at a molecular level the mechanism of uptake of such proteins, we have investigated the binding to coatomer of p24 proteins as examples of cycling proteins as well as that of ER-resident cargos. The p24 proteins required dimerization to interact with coatomer at two independent binding sites in gamma-COP. In contrast, ER-resident cargos bind to coatomer as monomers and to sites other than gamma-COP. The COPI coat therefore discriminates between p24 proteins and ER-resident proteins by differential binding involving distinct subunits.
authors.affiliation: Biochemie-Zentrum der Universität Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany. Julien.Bethune@bzh.uni-heidelberg.de
authors.forename: Julien
authors.initials: J
authors.lastname: Béthune
authors.forename: Matthijs
authors.initials: M
authors.lastname: Kol
authors.forename: Julia
authors.initials: J
authors.lastname: Hoffmann
authors.forename: Inge
authors.initials: I
authors.lastname: Reckmann
authors.forename: Britta
authors.initials: B
authors.lastname: Brügger
authors.forename: Felix
authors.initials: F
authors.lastname: Wieland
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D022181:COP-Coated Vesicles / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D020756:Coatomer Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019281:Dimerization; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D037241:Mannose-Binding Lectins / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D002352:Carrier Proteins; D020756:Coatomer Protein; D037241:Mannose-Binding Lectins; D008565:Membrane Proteins; D010455:Peptides; D020033:Protein Isoforms