title: Bacterial profile in primary teeth with necrotic pulp and periapical lesions.
abstract: The objective of this study was to evaluate the bacterial profile in root canals of human primary teeth with necrotic pulp and periapical lesions using bacterial culture. A total of 20 primary teeth with necrotic pulp and radiographically visible radiolucent areas in the region of the bone furcation and/or the periapical region were selected. After crown access, 4 sterile absorbent paper points were introduced sequentially into the root canal for collection of material. After 30 s, the paper points were removed and placed in a test tube containing reduced transport fluid (RTF) and were sent for microbiological evaluation. Anaerobic microorganisms were found in 100% of the samples, black-pigmented bacilli in 30%, aerobic microorganisms in 60%, streptococci in 85%, gram-negative aerobic rods in 15% and staphylococci were not quantified. Mutans streptococci were found in 6 root canals (30%), 5 canals with Streptococcus mutans and 1 canal with Streptococcus mutans and Streptococcus sobrinus. It was concluded that in root canals of human primary teeth with necrotic pulp and periapical lesions, the infection is polymicrobial with predominance of anaerobic microorganisms.
authors.affiliation: Department of Pediatric Clinics, Preventive and Social Dentistry, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Léa Assed Bezerra
authors.initials: LA
authors.lastname: da Silva
authors.forename: Paulo
authors.initials: P
authors.lastname: Nelson-Filho
authors.forename: Gisele
authors.initials: G
authors.lastname: Faria
authors.forename: Maria Cristina Monteiro
authors.initials: MC
authors.lastname: de Souza-Gugelmin
authors.forename: Izabel Yoko
authors.initials: IY
authors.lastname: Ito
mesh_terms: D002648:Child; D002675:Child, Preschool; D003786:Dental Pulp Cavity / Q000382:microbiology*; D003790:Dental Pulp Necrosis / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D010483:Periapical Diseases / Q000382:microbiology*; D013295:Streptococcus mutans / Q000302:isolation & purification; D016986:Streptococcus sobrinus / Q000302:isolation & purification; D014094:Tooth, Deciduous / Q000382:microbiology*