[Asparagine synthetase activity in pediatric acute lymphoblastic leukemia].
OBJECTIVE
To study the cellular activity of asparagine synthetase in different types of childhood acute lymphoblastic leukemia (ALL).


METHODS
The cellular activity of asparagine synthetase was detected by HPLC-FLD and Protein measurement in 28 ALL children (7 cases of T-ALL and 21 cases of B-lymphoid lineage ALL) before chemotherapy.


RESULTS
The asparagines synthetase activity levels in T-ALL children were significantly higher than those of the B-lymphoid lineage ALL patients, with the median activity level of 9.3 nM Asn/mg protein/hr vs 5.2 nM Asn/mg protein/hr (P < 0.05). The distribution of the asparagine synthetase activity demonstrated a polymorphism in either T-ALL or B-lymphoid lineage ALL patients.


CONCLUSIONS
The cellular activity of asparagines synthetase in ALL patients is presented with a polymorphism distribution. The asparagines synthetase activity levels in T-ALL are significantly higher than in B-lymphoid lineage ALL.
Jiang
Hua
H
Department of Hematology/Oncology, Shanghai Children's Medical Center Affiliated to Medical College of Shanghai Jiaotong University, Shanghai 200127, China. jiang_hua18@yahoo.com.cn
Gu
Long-Jun
LJ
Xue
Hui-Liang
HL
Tang
Jing-Yan
JY
Chen
Jing
J
Pan
Ci
C
Chen
Jing
J
D001215:Asparaginase / Q000627:therapeutic use; D001217:Aspartate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism*; D002648:Child; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis; D000097764:ETS Translocation Variant 6 Protein
D001215:Asparaginase; D001217:Aspartate-Ammonia Ligase; D050676:Core Binding Factor Alpha 2 Subunit; D015514:Oncogene Proteins, Fusion; D012333:RNA, Messenger; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein