Multilayer transfer printing on microreservoir-patterned substrate employing hydrophilic composite mold for selective immobilization of biomolecules.
In this study, we introduce a hydrophilic composite mold with elasticity and moderate water permeability, suitable for transferring water-soluble polar molecules such as polyelectrolyte multilayer. This composite mold is constructed from two UV-curable polymers-Norland Optical Adhesives (NOA) 63, a urethane-related polymer, and poly(ethylene glycol) diacrylate (PEGDA). The mixture of inherently hard NOA 63 and hydrogel precursor, PEGDA, resulted in an optically transparent mold with some degree of elasticity and enhanced water permeability upon UV polymerization. Employing the NOA 63-PEGDA composite mold, a polyelectrolyte multilayer comprising alternate thin layers of poly(acrylic acid) (PAA) and poly(acrylamide) (PAAm) was transfer-printed onto arrays of microreservoir-patterned substrate to selectively prevent unwanted adsorption of biomolecules on the protruding surface. Antibody was immobilized selectively inside the microreservoirs where multilayer was not transferred, and a specific antibody binding reaction was detected inside the microreservoirs. Furthermore, the potential of this composite mold as a convenient tool for constructing a biosensor for detecting Escherichia coli (E. coli) O157:H7 was explored.
Lee
Nae Yoon
NY
Center for Intelligent Nano-Bio Materials, Division of Nano Sciences (BK 21) and Department of Chemistry, Ewha Womans University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul 120-750, Korea.
Lim
Ju Ri
JR
Lee
Min Jung
MJ
Park
Sungsu
S
Kim
Youn Sang
YS
D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D004926:Escherichia coli; D057927:Hydrophobic and Hydrophilic Interactions; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry
D000906:Antibodies; D011140:Polyurethanes; C437167:poly(ethylene glycol)diacrylate; D011092:Polyethylene Glycols