title: [Molecular forms of TFF1 in normal gastric mucosa and its relationship with canceration].
abstract: OBJECTIVE
  To study the molecular forms of TFF1 in normal gastric mucosa and its expression in normal, gastric carcinoma, atypical hyperplasia, and intestinalized gastric mucosa.
  
  
  METHODS
  The molecular forms of TFF1 in normal gastric mucosa was observed by western blotting. The expression of TFF1 in normal, gastric carcinoma, atypical hyperplasia, and intestinalization gastric mucosa was assayed immunohistochemically.
  
  
  RESULTS
  TFF1 existed in normal gastric mucosa in forms of monomer, dimer and 21-kD TFF1 complex, with the last being the richest. TFF1 was expressed mainly in the epithelial cytoplasm of the mucosa in the gastric body and antrum, especially around the nucleus, and the closer to the lumen, the higher the expression. TFF1 expression in the tissues adjacent to gastric carcinoma was higher than that in normal gastric mucosa (P<0.001), and the expression in gastric adenocarcinoma was positively correlated to differentiation of adenocarcinoma. No TFF1 was expressed in poorly differentiated adenocarcinoma. The expression of TFF1 in moderate and well differentiated adenocarcinoma was a little lower than that in normal mucosa (P>0.05). The gastric mucosa with atypical hyperplasia had significantly higher TFF1 expression than normal gastric mucosa (P<0.001), and TFF1 was not detected in intestinalized gastric mucosa. There was no significant difference in TFF1 expression between gastric mucosa around the intestinalized tissues and normal gastric mucosa (P>0.05).
  
  
  CONCLUSIONS
  TFF1 plays an important part in protection and restitution of the gastric mucosa, and TFF1 may be related to suppression and differentiation of carcinoma.
authors.affiliation: Department of Gastroenterology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. jianlin.ren@xmzsh.com
authors.forename: Jian-lin
authors.initials: JL
authors.lastname: Ren
authors.forename: Jin-yan
authors.initials: JY
authors.lastname: Luo
authors.forename: Ya-pi
authors.initials: YP
authors.lastname: Lu
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Jian-min
authors.initials: JM
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Hua-xiu
authors.initials: HX
authors.lastname: Shi
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins