Effect of rhodoxanthin from Potamogeton crispus L. on cell apoptosis in Hela cells.
Carotenoid, a natural functional pigment, is known to have anti-carcinogenic activity. To verify the anti-cancer effects of rhodoxanthin which is a kind of carotenoids, we investigated the effects of rhodoxanthin from Potamogeton crispus L. on the proliferation rate, cell cycle distribution, apoptosis and the change in mitochondrial membrane potential in Hela cell line. The effects of rhodoxanthin were also tested on the concentration of Ca(2+) in cells. Rhodoxanthin inhibited cell proliferation in Hela cells in a dose and time-dependent manner. Rhodoxanthin induced an accumulation of cells in the S phase of the cell cycle, reduced the mitochondria transmembrane potential and increased the concentration of intracellular Ca(2+). In summary, our results suggested that rhodoxanthin-induced apoptosis in Hela cells occurred via these pathways.
Ren
Dandan
D
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Peng
Guanghua
G
Huang
Hongxia
H
Wang
Haibin
H
Zhang
Shenghua
S
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004396:Coloring Agents; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D051336:Mitochondrial Membranes / Q000187:drug effects; D031821:Potamogetonaceae / Q000737:chemistry*; D011419:Propidium; D024341:Xanthophylls / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D024341:Xanthophylls; D011419:Propidium; C038054:rhodoxanthin; D002118:Calcium