A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts.
v-myb oncogene of avian myeloblastosis virus (AMV) transforms myelomonocytic cells in vitro and induces acute monoblastic leukemia in vivo. The transforming effect of the v-myb can be suppressed using phorbol ester (TPA) or histone deacetylase inhibitor trichostatin A (TSA), the inducers of cell differentiation that are in clinical trials. In this study, we used proteomics-based approach to identify proteins with variable expression in differentiated BM2 cells. Proteome variations induced by TPA and TSA were compared to examine the mechanism of differentiation-promoting effects of these drugs. We found that expression of several proteins participating in cell cytoskeleton rearrangement, heat shock response, proteosynthesis and cell signaling was altered in TPA- or TSA-treated cells. We present here the first comparative proteome analysis of v-myb-transformed monoblasts BM2 focused on identification of proteins involved in their terminal differentiation.
Ondrousková
Eva
E
Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, ILBIT, Pavilon A3, Kamenice 5, 625 00 Brno, Czech Republic.
Povolná
Karolína
K
Vána
Petr
P
Benes
Petr
P
Konecná
Hana
H
Zdráhal
Zbynek
Z
Smarda
Jan
J
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002461:Cell Line, Transformed; D002645:Chickens; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006877:Hydroxamic Acids / Q000494:pharmacology; D013058:Mass Spectrometry / Q000379:methods; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D020626:Oncogene Proteins v-myb / Q000187:drug effects / Q000502:physiology*; D010703:Phorbol Esters / Q000494:pharmacology; D011506:Proteins / Q000032:analysis* / Q000502:physiology; D040901:Proteomics / Q000379:methods*
D006877:Hydroxamic Acids; D020626:Oncogene Proteins v-myb; D010703:Phorbol Esters; D011506:Proteins; C012589:trichostatin A