Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part 4: growth of rat bone marrow stromal cells in three-dimensional hydrogels with positive and negative surface charges and in polyelectrolyte complexes.
The growth of bone marrow stromal cells was assessed in vitro in macroporous hydrogels based on 2-hydro- xyethyl methacrylate (HEMA) copolymers with different electric charges. Copolymers of HEMA with sodium methacrylate (MA(-)) carried a negative electric charge, copolymers of HEMA with [2-(methacryloyloxy)ethyl] trimethylammonium chloride (MOETA(-)) carried a positive electric charge and terpolymers of HEMA, MA(-) and MOETA(+) carried both, positive and negative electric charges. The charges in the polyelectrolyte complexes were shielded by counter-ions. The hydrogels had similar porosities, based on a comparison of their diffusion parameters for small cations as measured by the real-time tetramethylammonium iontophoretic method of diffusion analysis. The cell growth was studied in the peripheral and central regions of the hydrogels at 2 hours and 2, 7, 14 and 28 days after cell seeding. Image analysis revealed the highest cellular density in the HEMA-MOETA(+) copolymers; most of the cells were present in the peripheral region of the hydrogels. A lower density of cells but no difference between the peripheral and central regions was observed in the HEMA-MA(-) copolymers and in polyelectrolyte complexes. This study showed that positively charged functional groups promote the adhesion of cells.
Lesný
P
P
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Prádný
M
M
Jendelová
P
P
Michálek
J
J
Vacík
J
J
Syková
E
E
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology*; D004058:Diffusion; D004573:Electrolytes / Q000737:chemistry*; D005269:Femur / Q000378:metabolism; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007091:Image Processing, Computer-Assisted; D008689:Methacrylates / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology*; D013499:Surface Properties; D013997:Time Factors
D001672:Biocompatible Materials; D004573:Electrolytes; D008689:Methacrylates; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C005044:hydroxyethyl methacrylate