[Sensitivity profile of a panel of cell lines designed for the evaluation of in vitro cytotoxicity].
BACKGROUND
Preliminary in vitro cytoxicity evaluations are determined in human tumor cell lines as a bioassay for the screening of potentially anticancer natural products.


OBJECTIVE
To strengthen the available in vitro cytotoxicity evaluation models, the panel of cell lines was expanded, and the sensitivity profile of each cell line was evaluated for its response to selected antineoplasic drugs.


METHODS
HeLa, MKN-45 and U-937 cell lines were added to the panel, and the sensitivity was determined for each of seven cell lines: HEp-2, HT-29, MCF-7, SiHa, MKN-45, HeLa and U-937. The effects of the antineoplasic drugs Doxorubicin HCl, Taxol, Cisplatin, Cyclophosphamide and Carmustin were examined, using the methyl thiazol tetrazolium (MTT) reduction assay.


RESULTS
A differential sensitivity to the drugs Doxorubicin HCl, Taxol and Cisplatin was established among the cell lines by comparing the lethal concentration 50 (LC50) values. HEp-2 was the most sensitive cell line, whereas HeLa and U-937 were the most resistant. HEp-2 exhibited a biphasic response to Taxol treatment; this was related to the reported mechanism of action of this compound.


CONCLUSIONS
Cyclophosphamide and Carmustin did not show activity under test conditions.
León
Claudia Jhoana
CJ
Grupo de Farmacogenética del Cáncer, Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, D.C., Colombia.
Gómez
Sandra Milena
SM
Morantes
Sandra Johanna
SJ
Cordero
Claudia Patricia
CP
Aristizábal
Fabio Ancízar
FA
D000970:Antineoplastic Agents / Q000633:toxicity*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D012680:Sensitivity and Specificity
D000970:Antineoplastic Agents