title: Identification of Chlamydia pneumoniae proteins in the transition from reticulate to elementary body formation.
abstract: Chlamydia pneumoniae is an important human respiratory pathogen that is responsible for an estimated 10% of community-acquired pneumonia and 5% of bronchitis and sinusitis cases. We examined changes in global protein expression profiles associated with the redifferentiation of reticulate body (RB) to elementary body (EB) as C. pneumoniae cells progressed from 24 to 48 h postinfection in HEp2 cells. Proteins corresponding to those showing the greatest changes in abundance in the beginning of the RB to EB transition were then identified from purified EBs. Among the 300 spots recognized, 35 proteins that were expressed at sufficiently high levels were identified by mass spectrometry. We identified C. pneumoniae proteins that showed more than 2-fold increases in abundance in the early stages of RB to EB transition, including several associated with amino acid and cofactor biosynthesis (Ndk, TrxA, Adk, PyrH, and BirA), maintenance of cytoplasmic protein function (GroEL/ES, DnaK, DksA, GrpE, HtrA, ClpP, ClpB, and Map), modification of the bacterial cell surface (CrpA, OmpA, and OmcB), energy metabolism (Tal and Pyk), and the putative transcriptional regulator TctD. This study identified C. pneumoniae proteins involved in the process of redifferentiation into mature, infective EBs and indicates bacterial metabolic pathways that may be involved in this transition. The proteins involved in RB to EB transition are key to C. pneumoniae infection and are perhaps suitable targets for therapeutic intervention.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, University of Louisville, Louisville, Kentucky 40292, USA. mukhopadhyays.ctr.in@nmrc.navy.mil
authors.forename: Sanghamitra
authors.initials: S
authors.lastname: Mukhopadhyay
authors.forename: David
authors.initials: D
authors.lastname: Good
authors.forename: Richard D
authors.initials: RD
authors.lastname: Miller
authors.forename: James E
authors.initials: JE
authors.lastname: Graham
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Mathews
authors.forename: Peter
authors.initials: P
authors.lastname: Timms
authors.forename: James T
authors.initials: JT
authors.lastname: Summersgill
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis*; D002478:Cells, Cultured; D002690:Chlamydia Infections / Q000378:metabolism; D016993:Chlamydophila pneumoniae / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D015964:Gene Expression Regulation, Bacterial; D018507:Gene Expression Regulation, Developmental; D006801:Humans
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins