[Simultaneous determination of four compounds in Sanjing Shuanghuanglian Oral Liquid by high performance liquid chromatography-diode array detection-electrochemical detection].
Chlorogenic acid, caffeic acid, baicalin and luteolin in Sanjing Shuanghuanglian Oral Liquid were simultaneously detected and identified using a high performance liquid chromatography coupled with diode array detection and electrochemical detection (HPLC-DAD-ECD). The separation was performed on a Zorbax SB-C18 column (150 mm x 4.6 mm i. d., 5.0 microm). The mobile phase consisted of (A) methanol and (B) methanol-water-acetic acid (50: 50: 1, v/v/v) using a linear gradient elution of 2%A-3%A at 0-3 min, 3%A-25%A at 3-15 min, 25%A-80%A at 15-20 min. The flow rate was 0.8 mL/min. The DAD detection was used at 275 nm. The ECD detection was done at 0.7 V. The column thermostat set at 30 degrees C. The limits of detection of the 4 compounds were 1 mg/L for chlorogenic acid, 0.2 mg/L for caffeic acid, 9 mg/L for baicalin, 7 mg/L for luteolin. The average recoveries were between 96.6%-99.6% with relative standard deviations (RSDs) of 2.5%-4.1%. The method is simple, rapid, reproducible and accurate. It can be used for the routine analysis of the four compounds in Shuanghuanglian Oral Liquid.
Liu
Lin
L
Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an, China.
Suo
Zhirong
Z
Zheng
Jianbin
J
D002109:Caffeic Acids / Q000032:analysis*; D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D047311:Luteolin / Q000032:analysis*; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000737:chemistry; D011422:Propionates / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D010936:Plant Extracts; D011422:Propionates; D002726:Chlorogenic Acid; C038044:baicalin; D047311:Luteolin; C040048:caffeic acid