title: X-ray structural studies of the fungal laccase from Cerrena maxima.
abstract: Laccases are members of the blue multi-copper oxidase family. These enzymes oxidize substrate molecules by accepting electrons at a mononuclear copper centre and transferring them to a trinuclear centre. Dioxygen binds to the trinuclear centre and following the transfer of four electrons is reduced to two molecules of water. The X-ray structure of a laccase from Cerrena maxima has been elucidated at 1.9 A resolution using synchrotron data and the molecular replacement technique. The final refinement coefficients are Rcryst = 16.8% and Rfree = 23.0%, with root mean square deviations on bond lengths and bond angles of 0.015 A and 1.51 degrees , respectively. The type 1 copper centre has an isoleucine residue at the axial position and the "resting" state of the trinuclear centre comprises a single oxygen (OH) moiety asymmetrically disposed between the two type 3 copper ions and a water molecule attached to the type 2 ion. Several carbohydrate binding sites have been identified and the glycan chains appear to promote the formation of well-ordered crystals. Two tyrosine residues near the protein surface have been found in a nitrated state.
authors.affiliation: A.V. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskiy Prospect 59, 119333, Moscow, Russia.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Lyashenko
authors.forename: Isabel
authors.initials: I
authors.lastname: Bento
authors.forename: Viatcheslav N
authors.initials: VN
authors.lastname: Zaitsev
authors.forename: Nadezhda E
authors.initials: NE
authors.lastname: Zhukhlistova
authors.forename: Yuliya N
authors.initials: YN
authors.lastname: Zhukova
authors.forename: Azat G
authors.initials: AG
authors.lastname: Gabdoulkhakov
authors.forename: Ekaterina Y
authors.initials: EY
authors.lastname: Morgunova
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Voelter
authors.forename: Galina S
authors.initials: GS
authors.lastname: Kachalova
authors.forename: Elena V
authors.initials: EV
authors.lastname: Stepanova
authors.forename: Ol'ga V
authors.initials: OV
authors.lastname: Koroleva
authors.forename: Victor S
authors.initials: VS
authors.lastname: Lamzin
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Tishkov
authors.forename: Christian
authors.initials: C
authors.lastname: Betzel
authors.forename: Peter F
authors.initials: PF
authors.lastname: Lindley
authors.forename: Al'bert M
authors.initials: AM
authors.lastname: Mikhailov
mesh_terms: D001665:Binding Sites; D003300:Copper / Q000737:chemistry; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry*; D042845:Laccase / Q000737:chemistry*; D015394:Molecular Structure; D009566:Nitrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011487:Protein Conformation; D014443:Tyrosine / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; D009566:Nitrates; D011134:Polysaccharides; D014867:Water; D014443:Tyrosine; D003300:Copper; D042845:Laccase