title: Fast HPLC method for the determination of glimepiride, glibenclamide, and related substances using monolithic column and flow program.
abstract: This work presents a fast method for the simultaneous separation and determination of glimepiride, glibenclamide, and two related substances by RP LC. The separation was performed on a Chromolith Performance (RP-18e, 100 mm x 4.6 mm) column. As mobile phase, a mixture of phosphate buffer pH 3, 7.4 mM, and ACN (55:45 v/v) was used. Column oven temperature was set to 30 degrees C. The total chromatographic run time was 80 s. This was achieved using a flow program from 5 to 9.9 mL/min. Precisions of the interday and the intraday assay for both retention times and peak areas for the four analyzed compounds were less than 1.2%. The method showed good linearity and recovery. The short analysis time makes the method very valuable for quality control and stability testing of drugs and their pharmaceutical preparations.
authors.affiliation: Institute of Pharmaceutical Chemistry, TU Braunschweig, Braunschweig, Germany.
authors.forename: Sami
authors.initials: S
authors.lastname: El Deeb
authors.forename: Udo
authors.initials: U
authors.lastname: Schepers
authors.forename: Hermann
authors.initials: H
authors.lastname: Wätzig
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004355:Drug Stability; D005905:Glyburide / Q000031:analogs & derivatives / Q000032:analysis* / Q000592:standards; D007004:Hypoglycemic Agents / Q000032:analysis / Q000592:standards; D012015:Reference Standards; D013453:Sulfonylurea Compounds / Q000032:analysis* / Q000592:standards
chemical_list: D007004:Hypoglycemic Agents; D013453:Sulfonylurea Compounds; C057619:glimepiride; D005905:Glyburide