title: Lateral spread of an amplification signal using an enzymatic system on a conductive surface.
abstract: In this letter, we report a novel signal amplification phenomenon that rapidly and dramatically increases both the magnitude and the lateral extent of the original signal. This phenomenon utilizes an enzyme immobilized on a conductive surface to generate amplified signals at locations remote from the original site of enzyme activity. The result is demonstrated on a microfluidic platform using the established precipitating enzyme-substrate system of horseradish peroxidase (HRP) and 3,3',5,5'-tetramethylbenzidine (TMB) on a surface plasmon resonance (SPR) imaging system.
authors.affiliation: University of Washington, Department of Bioengineering, Box 355061, Seattle, Washington 98195, USA. hasenm@u.washington.edu
authors.forename: Melissa S
authors.initials: MS
authors.lastname: Hasenbank
authors.forename: Elain
authors.initials: E
authors.lastname: Fu
authors.forename: Paul
authors.initials: P
authors.lastname: Yager
mesh_terms: D004553:Electric Conductivity; D006735:Horseradish Peroxidase / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D006735:Horseradish Peroxidase