In silico analysis of genes nucleoprotein, neuraminidase and hemagglutinin: a comparative study on different strains of influenza A (Bird flu) virus sub-type H5N1.
The avian influenza (bird flu) is an infectious disease of birds, ranging from a mild to a severe form of illness. Influenza viruses pose significant challenges to both human and animal health. The proteins, nucleoprotein (NP), neuraminidase (NA) and hemagglutinin (HA) of influenza A virus (Bird flu virus) sub-type A/Hatay/2004/(H5N1) from chicken were selected for this study. Our in silico analysis predicted that HA of influenza A virus is highly sensitive to mutations and hence it is significant for its pathogenic nature. None of the mutations was detected as an important change except in NA where K332R was at a PKC phosphorylation site. Analysis of the sequence comparison showed that the maximum number of mutations were observed in HA. These mutations are significant as they are involved in change in polarity or hydrophobicity as well as in propensity of each amino acid residue to stabilize the secondary structure. The program MAPMUTATION can be used to monitor the mutations, and predict the trend of mutations.
Anwar
Tamanna
T
Distributed Information Sub-centre, Aligarh 202002, India.
Lal
Sunil K
SK
Khan
Asad U
AU
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics*; D053124:Influenza A Virus, H5N1 Subtype / Q000201:enzymology / Q000235:genetics*; D005585:Influenza in Birds; D009439:Neuraminidase / Q000737:chemistry / Q000235:genetics*; D009698:Nucleoproteins / Q000737:chemistry / Q000235:genetics*; D017433:Protein Structure, Secondary
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009698:Nucleoproteins; D009439:Neuraminidase