Distribution of CTLA-4 polymorphisms in allergic asthma.
BACKGROUND
The CTLA-4 molecule is an important negative regulator of T cell activation. It is encoded on chromosome 2q33 and found to be associated with several allergic phenotypes including asthma. However, the association of CTLA-4 gene polymorphisms with allergic asthma is still controversial and therefore was the subject of this study.


METHODS
By PCR-RFLP, the distribution of three single nucleotide polymorphisms (SNPs), -1147 C/T, -318 C/T, and +49 A/G, was examined in 219 Polish Caucasoid patients diagnosed with allergic asthma and in 102 ethnically matched healthy control individuals. (AT)(n) microsatellite polymorphism was also tested in the same individuals.


RESULTS
No statistically significant differences in SNPs or microsatellite allele, genotype or haplotype frequencies between patients and controls were found.


CONCLUSIONS
CTLA-4 polymorphisms do not seem to be a risk factor for allergic asthma in Poles.
Jasek
Monika
M
Laboratory of Immunogenetics, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Łuszczek
Wioleta
W
Obojski
Andrzej
A
Winiarska
Beata
B
Hałubek
Katarzyna
K
Nowak
Izabela
I
Mańczak
Maria
M
Wiśniewski
Andrzej
A
Pawlik
Andrzej
A
Jonkisz
Anna
A
Lebioda
Arleta
A
Majorczyk
Edyta
E
Dobosz
Tadeusz
T
Kuśnierczyk
Piotr
P
D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D000943:Antigens, Differentiation / Q000235:genetics* / Q000276:immunology; D001249:Asthma / Q000453:epidemiology* / Q000235:genetics*; D060908:CTLA-4 Antigen; D005787:Gene Frequency; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics*; D007166:Immunosuppressive Agents / Q000276:immunology; D011044:Poland / Q000453:epidemiology; D011110:Polymorphism, Genetic
D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D007166:Immunosuppressive Agents