Fas ligand in bull ejaculated spermatozoa:A quantitative immunocytochemical study.
Apoptosis, or programmed cell death, provides a way to remove redundant cells at the end of their lifespan and thus acts as a homeostatic mechanism, maintaining the correct number of cells in the body by balancing their production and death. In the testis, this process seemed to play a pivotal role in spermatogenesis. It is generally accepted that Sertoli cells control the germ cell population through one of the best-known apoptotic pathways, the Fas/Fas L paracrine signal transduction system, in which a Fas ligand (Fas L) expressed by Sertoli cells induces apoptosis when it binds with its receptor, Fas, expressed by the germ cells. Recently, we demonstrated the presence of Fas antigen in normal ejaculated spermatozoa from fertile bulls and suggested that this molecule might have a non-apoptotic, defensive role against injuries, especially oxidative stress. We have now investigated whether bull mature, fertile spermatozoa express not only the Fas receptor but also its natural ligand Fas L. Our results indicate that the whole sperm population expresses Fas L. We suggest that Fas L in bull spermatozoa, like in murine spermatozoa, might be able to kill activated lymphocytes and protect the male gamete from damage by the self-immune system or the cytotoxic activity of leukocytes in the female genital tract.
Porcelli
Franca
F
Istituto di Anatomia degli Animali Domestici, Facoltà di Medicina Veterinaria, Università degli Studi di Milano, Milano, Italy. franca.Porcelli@unimi.it
Meggiolaro
Daniela
D
Carnevali
Antino
A
Ferrandi
Bruno
B
D000818:Animals; D017209:Apoptosis; D002417:Cattle; D019610:Cytoprotection / Q000276:immunology; D004542:Ejaculation; D053222:Fas Ligand Protein / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D013094:Spermatozoa / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism
D053222:Fas Ligand Protein