Improved production of pristinamycin coupled with an adsorbent resin in fermentation by Streptomyces pristinaespiralis.
Batch fermentation by Streptomyces pristinaespiralis with the addition of adsorbent resins was used to increase the production of pristinamycin. In consideration of the adsorption capacity and the desorption ability, a polymeric resin, JD-1, was finally selected. The maximum production of pristinamycin in Erlenmeyer flasks went up to 1.13 from 0.4 g l(-1), by adding 12% (w/v) resin JD-1 into the culture broth at 20 h after inoculation. In a 3 l bioreactor, pristinamycin fermentation with the addition of 12% (w/v) resin JD-1 at 20 h after inoculation reached 0.8 g l(-1), which was a 1.25-fold increase over fermentation without resin.
Jia
Bo
B
Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou, 310027, PR China.
Jin
Zhi-Hua
ZH
Lei
Yin-Lin
YL
Mei
Le-He
LH
Li
Ning-Hui
NH
D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001671:Biochemistry / Q000379:methods; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D025762:Pristinamycin / Q000096:biosynthesis* / Q000737:chemistry; D012116:Resins, Plant / Q000737:chemistry*; D013302:Streptomyces / Q000378:metabolism*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D025762:Pristinamycin; D012116:Resins, Plant