Phenotypic and functional characterization of porcine granulocyte developmental stages using two new markers.
Here, we describe two new surface antigens, named 6D10 and 2B2, whose expression is restricted to porcine granulocytes. 6D10 is only detected in neutrophils and its expression decreases from promyelocytes to mature cells. By contrast, 2B2 antigen is selectively expressed in mature neutrophils, eosinophils and basophils. The expression of these antigens along granulocyte maturation allows the discrimination of several developmental stages of granulocytes based on phenotypic, morphological and functional characteristics previously established. Moreover, these new markers are useful tools to easily characterize the different granulocytes lineages (neutrophils, eosinophils and basophils). By using multiparameter flow cytometric analysis, we have performed a phenotypic and functional characterization of the granulocyte subsets identified by the combination of 6D10 and 2B2 antigens.
Pérez
C
C
Dpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain.
Revilla
C
C
Alvarez
B
B
Chamorro
S
S
Correa
C
C
Domenech
N
N
Alonso
F
F
Ezquerra
A
A
Domínguez
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D015214:Antigens, Differentiation, Myelomonocytic / Q000302:isolation & purification* / Q000378:metabolism; D001491:Basophils / Q000145:classification / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000145:classification; D004801:Eosine Yellowish-(YS); D004804:Eosinophils / Q000145:classification / Q000378:metabolism*; D005434:Flow Cytometry; D042381:Granulocyte Precursor Cells / Q000145:classification / Q000378:metabolism*; D015151:Immunoblotting; D008751:Methylene Blue; D009504:Neutrophils / Q000145:classification / Q000378:metabolism*; D013552:Swine
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; C043260:May-Grunwald Giemsa; D008751:Methylene Blue; D004801:Eosine Yellowish-(YS)