title: High-hydrostatic-pressure treatment impairs actin cables and budding in Saccharomyces cerevisiae.
abstract: We previously reported that the postgrowth of Escherichia coli K-12 after high-hydrostatic-pressure treatment (HPT) as moderate as 75 MPa for 30 min at 37 degrees C induced the formation of elongated cells due to an HPT-induced disorder in FtsZ ring formation, which is essential for cell division. Because an FtsZ ring is known as a bacterial cytoskeleton, we examined the effect of HPT on a eukaryotic cytoskeleton, such as actin cables (long bundles of actin filaments), of Saccharomyces cerevisiae. We found that actin cables disappeared after HPT (100 MPa) and were not reorganized until 3.5 h of growth after HPT. As long as actin cables disappeared, budding did not start. We also demonstrated that the in vitro polymerization of actin monomers was highly sensitive to HPT.
authors.affiliation: Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Taketo
authors.initials: T
authors.lastname: Kawarai
authors.forename: Seisuke
authors.initials: S
authors.lastname: Arai
authors.forename: Soichi
authors.initials: S
authors.lastname: Furukawa
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ogihara
authors.forename: Makari
authors.initials: M
authors.lastname: Yamasaki
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003599:Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure*; D040542:Mechanotransduction, Cellular / Q000502:physiology; D011312:Pressure; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000502:physiology*
chemical_list: D000199:Actins