Tcof1/Treacle is required for neural crest cell formation and proliferation deficiencies that cause craniofacial abnormalities.
Neural crest cells are a migratory cell population that give rise to the majority of the cartilage, bone, connective tissue, and sensory ganglia in the head. Abnormalities in the formation, proliferation, migration, and differentiation phases of the neural crest cell life cycle can lead to craniofacial malformations, which constitute one-third of all congenital birth defects. Treacher Collins syndrome (TCS) is characterized by hypoplasia of the facial bones, cleft palate, and middle and external ear defects. Although TCS results from autosomal dominant mutations of the gene TCOF1, the mechanistic origins of the abnormalities observed in this condition are unknown, and the function of Treacle, the protein encoded by TCOF1, remains poorly understood. To investigate the developmental basis of TCS we generated a mouse model through germ-line mutation of Tcof1. Haploinsufficiency of Tcof1 leads to a deficiency in migrating neural crest cells, which results in severe craniofacial malformations. We demonstrate that Tcof1/Treacle is required cell-autonomously for the formation and proliferation of neural crest cells. Tcof1/Treacle regulates proliferation by controlling the production of mature ribosomes. Therefore, Tcof1/Treacle is a unique spatiotemporal regulator of ribosome biogenesis, a deficiency that disrupts neural crest cell formation and proliferation, causing the hypoplasia characteristic of TCS craniofacial anomalies.
Dixon
Jill
J
School of Dentistry and Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom. dixon@manchester.ac.uk
Jones
Natalie C
NC
Sandell
Lisa L
LL
Jayasinghe
Sachintha M
SM
Crane
Jennifer
J
Rey
Jean-Philippe
JP
Dixon
Michael J
MJ
Trainor
Paul A
PA
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D049109:Cell Proliferation; D019465:Craniofacial Abnormalities / Q000196:embryology / Q000235:genetics* / Q000473:pathology*; D003433:Crosses, Genetic; D046149:Embryo Culture Techniques; D006579:Heterozygote; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008817:Mice, Mutant Strains; D009432:Neural Crest / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000032:analysis / Q000378:metabolism
D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D012335:RNA, Ribosomal; C491352:Tcof1 protein, mouse