title: A newly designed automatic pH-cycling system to simulate daily pH fluctuations.
abstract: The aim of this study was to develop and evaluate an automatic pH-cycling system, which was intended for performing time-lapse analyses of dental caries. Two single-section specimens prepared from each of the five extracted human incisors were studied under a condition of 3- or 9-cycles/day for seven weeks. Increase of mineral loss (delta delta Z) and increase of Ld (delta Ld) were examined and compared at the end of every week during the study. In the 3-cycles/day condition, delta delta Z and delta Ld significantly increased only at Week 7. In the 9-cycles/day condition, delta deltaZ increased significantly from Week 5. delta Ld increased from Week 2, and then there were no further increases for the subsequent five weeks. Based on the results of this study, progression pattern was found to be significantly different between the two conditions. We therefore concluded that the automatic pH-cycling system which we have developed qualified as a model system that simulated the human oral cavity. As such, it could be beneficially employed to clarify the roles of many factors that cause or prevent caries development in human teeth.
authors.affiliation: Division of Oral Health Science, Department of Restorative Dentistry, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan. matsuda@den.hokudai.ac.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Hisanori
authors.initials: H
authors.lastname: Komatsu
authors.forename: Yukie
authors.initials: Y
authors.lastname: Murata
authors.forename: Toru
authors.initials: T
authors.lastname: Tanaka
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Sano
mesh_terms: D000704:Analysis of Variance; D002940:Circadian Rhythm; D003731:Dental Caries / Q000175:diagnosis*; D003732:Dental Caries Activity Tests / Q000295:instrumentation / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009055:Mouth / Q000737:chemistry; D017001:Tooth Demineralization; D014089:Tooth Remineralization