Monoclonal antibodies against chicken interleukin-6.
Monoclonal antibodies (mAb) were produced against a recombinant (r) chicken interleukin-6 (IL-6). Eight mAbs produced were tested for isotype; ability to inhibit recombinant forms of chicken (ch), human (h) and murine (m) IL-6; and recognition of rchIL-6 by Western immunoblotting. The mAb isotypes were represented by IgG1 (one), IgG2a (six) and IgG2b (one). In a mouse B9 hybridoma cell bioassay with rmIL-6, four mAbs effectively inhibited activity of rmIL-6. Further bioassays with the four mAbs at varying concentrations showed that two of these mAbs (1.20.7 and 1.26.4) were quite effective at inhibiting rmIL-6. Recombinant forms of ch, h and mIL-6 were all tested in a bioassay with the most potent inhibiting mAb (1.26.4), and this mAb was effective in inhibiting all three recombinant IL-6 proteins. Western immunoblotting revealed identification of the original IL-6 immunogen used for mAb production. Based upon inhibition of IL-6 activity in a standard bioassay and IL-6 recognition by Western immunoblotting, mAb 1.26.4 was judged the most useful antibody for future studies and applications.
Scott
T R
TR
Department of Animal & Veterinary Sciences, Clemson University, 123 P&AS Building, Clemson, SC 29634-0311, USA. trscott@clemson.edu
Lillehoj
H S
HS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology / Q000494:pharmacology; D001681:Biological Assay / Q000662:veterinary; D015153:Blotting, Western / Q000662:veterinary; D002352:Carrier Proteins / Q000276:immunology; D002645:Chickens / Q000276:immunology*; D005260:Female; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology*; D058619:Maltose-Binding Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D007130:Immunoglobulin Idiotypes; D015850:Interleukin-6; D058619:Maltose-Binding Proteins; D011994:Recombinant Proteins