[Construction of RNAi binary vector of soybean receptor-like kinase gene (rlpk2) and its soybean transformation].
Plant receptor-like kinases (RLKs) play important roles in plant growth, development and responses to environmental stress. RNA interference (RNAi) is a powerful tool to study functions of RLKs. In this study, a 312bp 3'end cDNA fragment of the soybean receptor-like kinase gene rlpk2 was chosen to construct the rlpk2-RNAi expression cassette. The binary vector pART27-R2 harboring rlpk2-RNAi expression cassette was constructed through three times subcloning via mid-clone vector and then transformed into Agrobacterium LBA4404. Three independent transgenic plants were obtained by Agrobactium-mediated soybean cotyledon transformation method. RT-PCR analysis showed that rlpk2 gene was successfully knocked down in all transgenic plants. It was found that photosynthesis activities of rlpk2-RNAi transgenic leaves were much improved, suggesting rlpk2 may function as a negative regulator of soybean leaf functions and/or chloroplast structure.
Li
Xiao Ping
XP
Plant Biology and Ecology Department, Nan Kai university, Tianjin 300071.
Deng
Nan
N
Ma
Yuan Yuan
YY
Li
Peng Li
PL
Wang
Yong
Y
Zhang
Ren
R
Wang
Ning Ning
NN
D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics*; D008957:Models, Genetic; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014170:Transformation, Genetic / Q000235:genetics*
D010940:Plant Proteins; D034741:RNA, Small Interfering