The effect of purified micronized flavonoid fraction on the healing of anastomoses in the colon in rats.
OBJECTIVE
Anastomotic leakage of colonic and rectal anastomoses is a major complication after large intestine surgery. Many factors influence the healing of colon anastomoses. Flavonoids have been recognized for centuries as physiologically active constituents that are used to treat human diseases. We studied the effects of a clinically used, micronized, purified flavonoid fraction on the healing of colonic anastomosis in rats.


METHODS
Male Sprague-Dawley rats were used. The flavonoid group of rats received 100 mg/kg per day of Daflon for 14 days until surgery. Thereafter, a resection and anastomosis were performed. The bursting pressure of the anastomoses and the hydroxyproline levels of the perianastomotic tissue were determined to evaluate the healing on the third and seventh days of surgery for both flavonoid and control groups.


RESULTS
The bursting pressure of the flavonoid group was higher on the seventh day. The hydroxyproline levels of the flavonoid group were significantly higher than in the control group on both the third and seventh days after surgery.


CONCLUSIONS
Although the micronized purified flavonoid fraction has some inhibitory properties on the healing of the anastomosis, its net effect was to obtain a better anastomotic healing of the colon in rats.
Inan
Aydin
A
Department of General Surgery, Fatih University School of Medicine, Beştepe, Emek 06510, Ankara, Türkiye.
Sen
Meral
M
Koca
Cemile
C
Akpinar
Ayhan
A
Dener
Cenap
C
D000714:Anastomosis, Surgical / Q000009:adverse effects*; D000818:Animals; D003094:Collagen / Q000187:drug effects; D004145:Diosmin / Q000494:pharmacology / Q000627:therapeutic use*; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013529:Surgical Wound Dehiscence / Q000517:prevention & control*; D014945:Wound Healing / Q000187:drug effects*
D005419:Flavonoids; D004145:Diosmin; D003094:Collagen