title: Analysis of gene expression identifies candidate molecular markers in nasopharyngeal carcinoma using microdissection and cDNA microarray.
abstract: OBJECTIVE
  Microarray analysis was used to bring a comprehensive insight into underlying molecular mechanisms and obtain a whole assessment of aberrant gene expression in nasopharyngeal carcinoma (NPC).
  
  
  METHODS
  Combined with microdissection, gene expression profiles in 23 NPCs and 10 nontumor nasopharyngeal epithelial tissue samples were analyzed.
  
  
  RESULTS
  Gene expression patterns suggested the dysregulation of the GTP/GDP-bound Ras cycle and an abnormal hyperactivity of cell cycle in NPC. Alterations in the WNT pathway suggest that this pathway may be activated in NPC. A 6-feature weighted-voting model was chosen because it represented the main characteristics of NPCs and predicted NPCs most accurately from the nontumor tissues (33 of 34 correct calls; 97.1% accuracy, Fisher's exact test, P value = 8.389 x 10(-8)).
  
  
  CONCLUSIONS
  The data generated in this study represent a comprehensive list of genes aberrantly regulated in NPC. The 6-feature weighted-voting model may provide an extensive list of potential molecular markers for early diagnosis.
authors.affiliation: Cancer Research Institute, Central South University, Changsha, Hunan, 410078, People's Republic of China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Zeng
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Luo
authors.forename: Wenling
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Li
authors.forename: Songqing
authors.initials: S
authors.lastname: Fan
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.forename: Ke
authors.initials: K
authors.lastname: Tang
authors.forename: Minghua
authors.initials: M
authors.lastname: Wu
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002277:Carcinoma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D016000:Cluster Analysis; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D042282:Microdissection / Q000379:methods; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor