LRRC4 controls in vitro invasion of glioblastoma cells through inhibiting RPTP-zeta expression.
LRRC4 (leucine rich repeat containing 4), a novel member of LRP (Leucine-rich repeat protein) superfamily, contains a conserved leucine-rich repeat (LRR) cassette and an immunoglobulin-like (IgC2) domain in its extracellular region. In the present study, we demonstrated that the N and C terminal LRR (LRRNT and LRRCT) are requisite for membrane and cytoplasm location of LRRC4 in Cos7 cells. We also suggested that RPTP-zeta (receptor-type protein tyrosine phosphatase) receptor is relevant to the invasion ability of gliomas cells, and its expression is inhibited by the reexpression of LRRC4. Our observations indicated that LRRC4 may be a negative regulator of the RPTP-zeta receptor, and contribute to suppressing the invasion ability of gliomas cells.
Wu
Minghua
M
Cancer Research Institute, Central South University, Changsha, 410078 Hunan, People's Republic of China.
Gan
Kai
K
Huang
Chen
C
Tang
Yunlian
Y
Chen
Qiong
Q
Tang
Ke
K
Li
Xiaoling
X
Shen
Shourong
S
Li
Guiyuan
G
D000595:Amino Acid Sequence; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D016543:Central Nervous System Neoplasms / Q000235:genetics* / Q000473:pathology*; D015194:Diffusion Chambers, Culture; D017629:Gap Junctions / Q000502:physiology; D017353:Gene Deletion; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000502:physiology; D006801:Humans; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D011993:Recombinant Fusion Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012608:Scrapie / Q000473:pathology; D014162:Transfection
C503331:LRRC4 protein, human; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C516895:PTPRZ1 protein, human; D017027:Protein Tyrosine Phosphatases; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5