A fluorometric study of relative ocular lens cytosensitivity to multipurpose contact lens solutions using the resazurin assay method.
This study investigated the relative ocular lens cytosensitivity to three multipurpose contact lens solutions using lens culture and resazurin microplate assay method. Comparison in the cell viability levels of the cultured bovine lenses as influenced by 3h experimental exposure to three contact lens multipurpose solutions (OPTI-FREE Express, Alcon; ReNu MultiPlus, Bausch & Lomb; and COMPLETE Comfort Plus, Allergan) was performed. The pre- and post-exposure fluorescence emission levels of the lenses were obtained, and findings were compared to baseline and control measurements. The solutions demonstrated varying levels of sensitivity (P<0.1), exhibiting non-significant to significant reversible disruption of the lenses'cell viability levels as revealed by the fluorescence intensity levels in the following ranked order (COMPLETE< ReNu< OPTI-FREE solutions). The findings show that OPTI-FREE Express and ReNu MultiPlus solutions exhibited more cytosensitive effect compared to COMPLETE Comfort Plus. These results suggest that the resazurin assay together with lens culture would be a reliable system for in vitro investigation of the effect of contact lens solutions and other ophthalmic formulations on ocular tissues.
Oriowo
Mathew Olanrewaju
MO
Department of Optometry and Vision Science, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia. moriowo@hotmail.com
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003228:Conjunctiva / Q000166:cytology; D018639:Contact Lens Solutions / Q000633:toxicity*; D004847:Epithelial Cells / Q000187:drug effects; D005470:Fluorometry; D007202:Indicators and Reagents; D007908:Lens, Crystalline / Q000187:drug effects* / Q000196:embryology; D008856:Microscopy, Fluorescence; D010078:Oxazines; D014966:Xanthenes
D018639:Contact Lens Solutions; D007202:Indicators and Reagents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin