Non-involvement of the human monocarboxylic acid transporter 1 (MCT1) in the transport of phenolic acid.
Phenolic acids such as p-coumaric acid and microbial metabolites of poorly absorbed polyphenols are absorbed by the monocarboxylic acid transporter (MCT)-mediated transport system which is identical to the fluorescein/H(+) cotransport system. We focus here on the physiological impact of MCT-mediated absorption and distribution. We examined whether MCT1, the best-characterized isoform found in almost all tissues, is involved in this MCT-mediated transport system. The induction of MCT1 expression in Caco-2 cells by a treatment with sodium butyrate (NaBut) did not increase the fluorescein permeability. Moreover, the transfection of Caco-2 cells with an expression vector encoding MCT1 caused no increase in either the permeability or uptake of fluorescein. Furthermore, in the MCT1-expressing oocytes, no increase of p-coumaric acid uptake was apparent, whereas the uptake of salicylic acid, a substrate of MCT1, nearly doubled. Our data therefore establish that MCT1 was not involved in the MCT-mediated transport of phenolic acids.
Watanabe
Hirohito
H
Department of Life Sciences, Faculty of Agriculture, Meiji University, Kanagawa, Japan.
Yashiro
Takuya
T
Tohjo
Yuichi
Y
Konishi
Yutaka
Y
D002087:Butyrates / Q000494:pharmacology; D018938:Caco-2 Cells; D003373:Coumaric Acids / Q000493:pharmacokinetics; D005452:Fluoresceins / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D062385:Hydroxybenzoates / Q000378:metabolism*; D027501:Monocarboxylic Acid Transporters / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000187:drug effects; D010539:Permeability; D011422:Propionates; D020156:Salicylic Acid; D027981:Symporters / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured; D000099233:Monocarboxylate Transport Protein 1
D002087:Butyrates; D003373:Coumaric Acids; D005452:Fluoresceins; D062385:Hydroxybenzoates; D027501:Monocarboxylic Acid Transporters; D011422:Propionates; D020156:Salicylic Acid; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1; C017616:phenolic acid; C495469:p-coumaric acid