title: Determination of nitroimidazole residues in porcine urine by liquid chromatography/tandem mass spectrometry.
abstract: A reliable and sensitive method was developed for determination of nitroimidazoles in porcine urine. Sample preparation involves an extraction with ethyl acetate, followed by a solid-phase extraction cleanup step. The final extract is injected into the liquid chromatography/tandem mass spectrometry system in the positive-ion electrospray ionization mode. A C8 column with water and acetonitrile as the mobile phase is used for chromatographic separation under gradient conditions. Overall average recoveries ranged from 83 to 107%. The limits of detection ranged from 0.03 to 0.05 ng/mL, and the limits of quantitation, from 0.1 to 0.2 ng/mL.
authors.affiliation: China Agricultural University, College of Veterinary Medicine, Department of Pharmacology and Toxicology, Beijing, 100094, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Xia
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
authors.forename: Suxia
authors.initials: S
authors.lastname: Zhang
authors.forename: Shuangyang
authors.initials: S
authors.lastname: Ding
authors.forename: Haiyang
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*; D009593:Nitroimidazoles / Q000032:analysis* / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013552:Swine; D013696:Temperature; D014556:Urine
chemical_list: D007477:Ions; D009593:Nitroimidazoles; D012997:Solvents