[Identification of glufosfamide metabolites in rats].
OBJECTIVE
To elucidate the metabolic pathway of glufosfamide in rats.


METHODS
In this study, a liquid chromatography-tandem mass spectrometric method was developed and applied to characterize the metabolites of glufosfamide in rat urine, after an i.v. administration of 50 mg x kg(-1). The analysis was performed under two ionization modes in two different chromatographic systems, separately. To make sure that the compounds detected in rat urine were metabolites or degradation products, the stability of glufosfamide, isophosphoramide mustard (M1), and the degradation products of M1 in urine were investigated.


RESULTS
In positive ionization mode, besides glufosfamide, two metabolites, isophosphoramide mustard and monoaziridinyl derivative of isophosphoramide mustard, were detected. In negative ionization mode, only glufosfamide itself was detected, while derivatives of isophosphoramide mustard have no response in such condition.


CONCLUSIONS
Glufosfamide was mainly unchanged excreted in urine, and two metabolites were detected as isophosphoramide mustard and monoaziridinyl derivative of isophosphoramide mustard.
Sun
Yu-Ming
YM
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Chen
Xiao-Yan
XY
Zhong
Da-Fang
DF
D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000378:metabolism* / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000031:analogs & derivatives; D007069:Ifosfamide / Q000031:analogs & derivatives; D007275:Injections, Intravenous; D008297:Male; D010752:Phosphoramide Mustards / Q000378:metabolism* / Q000652:urine; D051381:Rats; D017208:Rats, Wistar
D018906:Antineoplastic Agents, Alkylating; D010752:Phosphoramide Mustards; C092028:beta-D-glucosylisophosphoramide mustard; C027061:isophosphamide mustard; D005947:Glucose; D007069:Ifosfamide