X-ray structural studies of the fungal laccase from Cerrena maxima.
Laccases are members of the blue multi-copper oxidase family. These enzymes oxidize substrate molecules by accepting electrons at a mononuclear copper centre and transferring them to a trinuclear centre. Dioxygen binds to the trinuclear centre and following the transfer of four electrons is reduced to two molecules of water. The X-ray structure of a laccase from Cerrena maxima has been elucidated at 1.9 A resolution using synchrotron data and the molecular replacement technique. The final refinement coefficients are Rcryst = 16.8% and Rfree = 23.0%, with root mean square deviations on bond lengths and bond angles of 0.015 A and 1.51 degrees , respectively. The type 1 copper centre has an isoleucine residue at the axial position and the "resting" state of the trinuclear centre comprises a single oxygen (OH) moiety asymmetrically disposed between the two type 3 copper ions and a water molecule attached to the type 2 ion. Several carbohydrate binding sites have been identified and the glycan chains appear to promote the formation of well-ordered crystals. Two tyrosine residues near the protein surface have been found in a nitrated state.
Lyashenko
Andrey V
AV
A.V. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskiy Prospect 59, 119333, Moscow, Russia.
Bento
Isabel
I
Zaitsev
Viatcheslav N
VN
Zhukhlistova
Nadezhda E
NE
Zhukova
Yuliya N
YN
Gabdoulkhakov
Azat G
AG
Morgunova
Ekaterina Y
EY
Voelter
Wolfgang
W
Kachalova
Galina S
GS
Stepanova
Elena V
EV
Koroleva
Ol'ga V
OV
Lamzin
Victor S
VS
Tishkov
Vladimir I
VI
Betzel
Christian
C
Lindley
Peter F
PF
Mikhailov
Al'bert M
AM
D001665:Binding Sites; D003300:Copper / Q000737:chemistry; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry*; D042845:Laccase / Q000737:chemistry*; D015394:Molecular Structure; D009566:Nitrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011487:Protein Conformation; D014443:Tyrosine / Q000737:chemistry; D014867:Water / Q000737:chemistry
D005656:Fungal Proteins; D009566:Nitrates; D011134:Polysaccharides; D014867:Water; D014443:Tyrosine; D003300:Copper; D042845:Laccase