Increased expression of a molecular chaperone GroEL in response to unsaturated fatty acids by the biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens.
Butyrivibrio fibrisolvens is the most active bacterial species in the biohydrogenation of polyunsaturated fatty acids (PUFA) in the rumen. It needs to remove the unsaturated bonds in order to detoxify the PUFA to enable the growth of the bacterium. Here, we investigated the response of cell membrane-associated proteins in B. fibrisolvens to growth in the presence of PUFA. Numerous changes were observed in the cell membrane-associated proteome. One of the main modifications occurring when the 18:2 fatty acids, linoleic acid and conjugated linoleic acid, were added, was an increased expression of the molecular chaperone GroEL.
Devillard
Estelle
E
Rowett Research Institute, Bucksburn, Aberdeen, UK. E.Devillard@rowett.ac.uk
Andant
Nicolas
N
John Wallace
R
R
D000595:Amino Acid Sequence; D000818:Animals; D045856:Butyrivibrio / Q000254:growth & development / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006865:Hydrogenation; D019787:Linoleic Acid / Q000378:metabolism* / Q000633:toxicity; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D040901:Proteomics; D012417:Rumen / Q000382:microbiology*; D016415:Sequence Alignment; D012756:Sheep; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D018834:Chaperonin 60; D008565:Membrane Proteins; D019787:Linoleic Acid