Reuse of the xylanase enzyme in the biobleaching process of the sugarcane bagasse acetosolv pulp.
In this work, pretreatment-enzymatic series of the bagasse-sugarcane pulp and alkaline extraction of enzyme treated pulp were carried out. In the pretreatment an enzyme dose was utilized and acetosolv pulp suspension of 3% (w/v) with different solvents (distilled water, 0.05 mol/L acetate buffer pH 5.5 and 0.05 mol/L phosphate buffer pH 7.25) stirred at 85 rpm for 2 or 4 h. The enzymes used were pulpzyme and cartazyme, both commercial. The accompaniment of the enzymatic activity was carried out through measurement in initial and finish of each enzymatic pretreatment. The xylanase-treated pulps and xylanase-alkaline-extracted pulps were analyzed regarding kappa number and viscosity. Pulpzyme recovery was better in phosphate buffered medium (84, 46, and 23% for first, second, and third enzymatic treatment, respectively) although in aqueous medium reached only 2% for every treatments. However, the improvement of pulp properties was evidenced only in aqueous medium for pulpzyme. Cartazyme recovery was similar for both solvents (water and acetate buffer), reaching values around 19% for first enzymatic treatment and 9% for second one. Nevertheless, the pulp properties increased only in acetate buffered medium.
Oliveira
Luís R M
LR
Biotechnology Department (DEBIQ)-FAENQUIL, CP 116, CEP 12600-970, Lorena-SP, Brazil.
Moriya
Regina Y
RY
Gonçalves
Adilson R
AR
D019342:Acetic Acid / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000302:isolation & purification*; D031786:Saccharum / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
D012997:Solvents; D002482:Cellulose; C027433:bagasse; D043364:Endo-1,4-beta Xylanases; D019342:Acetic Acid