Origin of second harmonic generation optical activity of a tryptophan derivative at the air/water interface.
Second harmonic generation optical activity (SHG-OA) of chiral monolayers of the tryptophan derivative N(alpha)-(tert-butoxycarbonyl)-tryptophan (BOC-Trp) at an air/water interface has been studied in detail. In combination with previously reported experimental measurements with the fundamental frequency variant Planck's 'h/' omega=2.20 eV (lambda=564 nm), new measurements with lambda=564 and 800 nm fully characterize the nonlinear susceptibility tensors of chiral and achiral (racemic) monolayers under two-photon resonant and nonresonant conditions of the fundamental frequency. A realistic computational approach including semiempirical, intermediate neglect of differential overlap (ZINDO/S) calculations has been used to calculate the nonlinear susceptibilities of model achiral and chiral monolayers composed of indole chromophores. There is satisfactory agreement between calculated and observed nonlinear susceptibilities, which constrains certain structural parameters of the monolayers including the absolute orientation of the long molecular axis of indole at the air/water interface. The origin of SHG-OA of BOC-Trp monolayers is discussed with reference of two distinct mechanisms at the microscopic level, designated type I or chiral assembly and type II or electronic coupling. Both mechanisms are studied in detail within the framework of ZINDO/S calculations. The dominant effect for the BOC-Trp monolayers is type I, involving chiral assembly of indole chromophores.
Mitchell
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Steacie Institute for Molecular Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario K1A0R6, Canada. steve.mitchell@nrc.gc.ca