title: Distribution of CTLA-4 polymorphisms in allergic asthma.
abstract: BACKGROUND
  The CTLA-4 molecule is an important negative regulator of T cell activation. It is encoded on chromosome 2q33 and found to be associated with several allergic phenotypes including asthma. However, the association of CTLA-4 gene polymorphisms with allergic asthma is still controversial and therefore was the subject of this study.
  
  
  METHODS
  By PCR-RFLP, the distribution of three single nucleotide polymorphisms (SNPs), -1147 C/T, -318 C/T, and +49 A/G, was examined in 219 Polish Caucasoid patients diagnosed with allergic asthma and in 102 ethnically matched healthy control individuals. (AT)(n) microsatellite polymorphism was also tested in the same individuals.
  
  
  RESULTS
  No statistically significant differences in SNPs or microsatellite allele, genotype or haplotype frequencies between patients and controls were found.
  
  
  CONCLUSIONS
  CTLA-4 polymorphisms do not seem to be a risk factor for allergic asthma in Poles.
authors.affiliation: Laboratory of Immunogenetics, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Jasek
authors.forename: Wioleta
authors.initials: W
authors.lastname: Łuszczek
authors.forename: Andrzej
authors.initials: A
authors.lastname: Obojski
authors.forename: Beata
authors.initials: B
authors.lastname: Winiarska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Hałubek
authors.forename: Izabela
authors.initials: I
authors.lastname: Nowak
authors.forename: Maria
authors.initials: M
authors.lastname: Mańczak
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wiśniewski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Pawlik
authors.forename: Anna
authors.initials: A
authors.lastname: Jonkisz
authors.forename: Arleta
authors.initials: A
authors.lastname: Lebioda
authors.forename: Edyta
authors.initials: E
authors.lastname: Majorczyk
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Dobosz
authors.forename: Piotr
authors.initials: P
authors.lastname: Kuśnierczyk
mesh_terms: D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D000943:Antigens, Differentiation / Q000235:genetics* / Q000276:immunology; D001249:Asthma / Q000453:epidemiology* / Q000235:genetics*; D060908:CTLA-4 Antigen; D005787:Gene Frequency; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics*; D007166:Immunosuppressive Agents / Q000276:immunology; D011044:Poland / Q000453:epidemiology; D011110:Polymorphism, Genetic
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D007166:Immunosuppressive Agents