Determination of chamazulene carboxylic acid in serum by high-performance liquid chromatography. Development and validation.
A new, simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for the determination of chamazulene carboxylic acid (CCA) in serum. The technique is based on a single liquid-liquid extraction of the substance using ibuprofen as internal standard (I.S.). The separation was achieved on a C(18) reversed-phase column using acetonitrile/water (4:6, pH 3) as mobile phase. The effluent was monitored at 221 and 286 nm. The calibration curves were linear over the concentration range of 0.1-30 microg/ml. The intra- and inter-day RSDs were in all cases less than 15 and 11%, respectively. The limit of quantitation was 0.1 microg/ml. The assay was developed and validated to be applied in a pharmacokinetic study in healthy volunteers.
Ramadan
Mai
M
Department of Pharmaceutical Chemistry, Philipps University, Marbacher Weg 6, D-35032 Marburg, Germany. farah_ar@hotmail.com
Watzer
Bernhard
B
D052176:Azulenes / Q000097:blood*; D002264:Carboxylic Acids / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry
D052176:Azulenes; D002264:Carboxylic Acids; D012997:Solvents; C013872:chamazulene