[Regulation of vitality and IL-10 receptor expression of human decidual stromal cells in early pregnancy by IL-10, IFN-gamma].
The study is to investigate the effect of IL-10, IFN-gamma on viability and IL-10 receptor expression of human decidual stromal cells (DSC) in early pregnancy. The vitality of DSC was detected by MTT. IL-10R1 and IL-10R gene expression of DSC were detected after treated by different concentrations of IL-10, IFN-gamma at 15min, 30min, 45min, 60min. The vitality of DSC was significantly enhanced by the higher dose of IL-10 (100-10ng/ml) (P<0.05), and there was no significant stimulative role induced by the lower dose of it(0.10-1ng/ml)(P>0.05). The viability of DSC was inhibited by the highest dose of IFN-gamma (1000ng/ml) (P<0.01), on the contrary, its viability was stimulated by the lower dose of IFN-gamma (10ng/ml) (P<0.05). IL-10R1 was highly expressed by IL-10 of 10ng/ml within 15min, and significantly reduced at 30min, then disappeared within 45min; while IL-10R1 expression was not induced by IL-10(1ng/ml) of lower concentration throughout 60min; IL-10R1 was briefly and lowly expressed by IFN-gamma of 100ng/ml, which effect on DSC at 45min; The expression of IL-10R1 was moderate level induced by IFN-gamma of 10ng/ml within 30min,and reduced at 45min,then disappeared at 60min. There was no significant difference of IL-10R2 expression (P>0.05) between treatment and not with the above-mentioned cytokines. IL-10, IFN-gamma may play an important role of immune regulation in early pregriancy by influencing IL-10R1 expression and vitality of DSC.
Xing
Chang Ying
CY
Obstetrics and Gynecology Hospital of Fudan University, Shanghai.
Gui
Sui Qi
SQ
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D016753:Interleukin-10 / Q000494:pharmacology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D053701:Receptors, Interleukin-10 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*
D053701:Receptors, Interleukin-10; D016753:Interleukin-10; D007371:Interferon-gamma