title: Beamline X29: a novel undulator source for X-ray crystallography.
abstract: A high-flux insertion device and beamline for macromolecular crystallography has been built at the National Synchrotron Light Source (NSLS) that employs a mini-gap undulator source developed by the NSLS. The mini-gap undulator at beamline X29 is a hybrid-magnet device of period 12.5 mm operating at proven gaps of 3.3-10 mm. The beamline provides hard X-rays for macromolecular crystallography experiments from the second and third harmonics over an energy range of 5-15 keV. The X-ray optics is designed to deliver intense and highly collimated X-rays. Horizontal focusing is achieved by a cryogenically cooled sagittally focusing double-crystal monochromator with approximately 4.1:1 demagnification. A vertical focusing mirror downstream of the monochromator is used for harmonic rejection and vertical focusing. The experimental station hosts an Area Detector Systems Quantum 315 CCD detector with 2.2 s readout time between exposures and Crystal Logic goniostat for crystal rotation and detector positioning. An auto-mounter crystal changer has been installed to facilitate the high-throughput data collection required by the major users, which includes structural genomics projects and the Macromolecular Crystallography Research Resource mail-in program. X29 is 10(3) times brighter than any existing bending-magnet beamline at NSLS with an actual flux of 2.5 x 10(11) photons s(-1) through a 0.12 mm square aperture at 11.271 keV.
authors.affiliation: Center for Synchrotron Biosciences, Case Proteomics Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
authors.forename: Wuxian
authors.initials: W
authors.lastname: Shi
authors.forename: Howard
authors.initials: H
authors.lastname: Robinson
authors.forename: Michael
authors.initials: M
authors.lastname: Sullivan
authors.forename: Don
authors.initials: D
authors.lastname: Abel
authors.forename: John
authors.initials: J
authors.lastname: Toomey
authors.forename: Lonny E
authors.initials: LE
authors.lastname: Berman
authors.forename: Don
authors.initials: D
authors.lastname: Lynch
authors.forename: Gerd
authors.initials: G
authors.lastname: Rosenbaum
authors.forename: George
authors.initials: G
authors.lastname: Rakowsky
authors.forename: Larry
authors.initials: L
authors.lastname: Rock
authors.forename: Bill
authors.initials: B
authors.lastname: Nolan
authors.forename: Grace
authors.initials: G
authors.lastname: Shea-McCarthy
authors.forename: Dieter
authors.initials: D
authors.lastname: Schneider
authors.forename: Erik
authors.initials: E
authors.lastname: Johnson
authors.forename: Robert M
authors.initials: RM
authors.lastname: Sweet
authors.forename: Mark R
authors.initials: MR
authors.lastname: Chance
mesh_terms: D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D009991:Oscillometry; D017356:Synchrotrons; D013997:Time Factors; D014965:X-Rays