Differentiation of rat bone marrow stem cells in liver after partial hepatectomy.
OBJECTIVE
To investigate the differentiation of rat bone marrow stem cells in liver after partial hepatectomy.


METHODS
Bone marrow cells were collected from the tibia of rat with partial hepatectomy, the medial and left hepatic lobes were excised. The bone marrow stem cells (Thy(+)CD3(-)CD45RA(-) cells) were enriched from the bone marrow cells by depleting red cells and fluorescence-activated cell sorting. The sorted bone marrow stem cells were labeled by PKH26-GL in vitro and autotransplanted by portal vein injection. After 2 wk, the transplanted bone marrow stem cells in liver were examined by the immunohistochemistry of albumin (hepatocyte-specific marker).


RESULTS
The bone marrow stem cells (Thy(+)CD3(-)CD45RA(-) cells) accounted for 2.8% of bone marrow cells without red cells. The labeling rate of 10 microM PKH26-GL on sorted bone marrow stem cells was about 95%. There were sporadic PKH26-GL-labeled cells among hepatocytes in liver tissue section, and some of the cells expressed albumin.


CONCLUSIONS
Rat bone marrow stem cells can differentiate into hepatocytes in regenerative environment and may participate in liver regeneration after partial hepatectomy.
Zhan
Yu-Tao
YT
Department of Gastroenterology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China. yutaozhan@263.net
Wang
Yu
Y
Wei
Lai
L
Liu
Bin
B
Chen
Hong-Song
HS
Cong
Xu
X
Fei
Ran
R
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006498:Hepatectomy / Q000379:methods*; D022781:Hepatocytes / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley