Effects of insemination-ovulation interval on fertilization rates and embryo characteristics in dairy cattle.
The objective of this study was to examine effects of the interval between insemination and ovulation on fertilization and embryo characteristics (quality scored as good, fair, poor and degenerate; morphology; number of cell cycles and accessory sperm number) in dairy cattle. Time of ovulation was assessed by ultrasonography (every 4h). Cows were artificially inseminated once between 36h before ovulation and 12h after ovulation. In total 122 oocytes/embryos were recovered 7d after ovulation. Insemination-ovulation interval (12h-intervals) affected fertilization and the percentages of good embryos. Fertilization rates were higher when AI was performed between 36-24 and 24-12h before ovulation (85% and 82%) compared to AI after ovulation (56%). AI between 24 and 12h before ovulation resulted in higher percentages of good embryos (68%) compared to AI after ovulation (6%). Insemination-ovulation interval had no effect on number of accessory sperm cells and number of cell cycles when corrected for embryo quality. This study showed that the insemination-ovulation interval with a high probability of fertilization is quite long (from 36 to 12h before ovulation). However, the insemination-ovulation interval in which this fertilized oocyte has a high probability of developing into a good embryo is shorter (24-12h before ovulation).
Roelofs
J B
JB
Adaptation Physiology Group, Wageningen University, P.O. Box 338, 6700 AH Wageningen, The Netherlands. judith.roelofs@wur.nl
Graat
E A M
EA
Mullaart
E
E
Soede
N M
NM
Voskamp-Harkema
W
W
Kemp
B
B
D000818:Animals; D002417:Cattle / Q000196:embryology / Q000502:physiology*; D005260:Female; D005306:Fertilization / Q000502:physiology*; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary; D008297:Male; D010060:Ovulation / Q000502:physiology*; D011247:Pregnancy; D013076:Sperm Count / Q000662:veterinary; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D013997:Time Factors