The background K(+) channel TASK-3 is regulated at both the transcriptional and post-transcriptional levels.
The K(+) channel TASK-3 is highly expressed in cerebellar granule neurons where it encodes the K(+) current IKso. Besides the role of TASK-3 in controlling cellular excitability and shaping neuronal responses, it has recently been proposed to contribute to the development and maturation of neurons in the cerebellum. K(+) dependent apoptosis and tumorigenesis have also been attributed to TASK-3 over-expression. Transcription of TASK-3 is strongly dependent on depolarization-induced Ca(2+)-entry. To understand the mechanisms involved in TASK-3 regulation, we have characterized a minimal promoter which specifically expresses in cellular backgrounds expressing endogenous TASK-3. Moreover, we have cloned and characterized the 5' and 3' untranslated regions of TASK-3. Both regions contribute to inhibit expression of a reporter gene. Given the direct consequence of membrane potential on TASK-3 expression, this is an important first step towards the understanding of the complex regulation of this gene.
Zanzouri
Marc
M
Institut de Pharmacologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, UMR 6097, Université de Nice-Sophia Antipolis, 660 Route des Lucioles, Valbonne 06560, France.
Lauritzen
Inger
I
Lazdunski
Michel
M
Patel
Amanda
A
D020413:3' Untranslated Regions; D024506:5' Flanking Region; D000818:Animals; D001483:Base Sequence; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D024683:Potassium Channels, Tandem Pore Domain / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051381:Rats; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
D020413:3' Untranslated Regions; C407082:Kcnk9 protein, rat; D024683:Potassium Channels, Tandem Pore Domain