[Preimplantation genetic diagnosis (PGD): results from a Parisian center].
OBJECTIVE
To report the results of preimplantation genetic diagnosis (PGD) cycles performed in our unit from 2000 to 2004. Materials and methods. One hundred and seventy-one couples were enrolled in the PGD program over this period. The collected oocytes were inseminated by intracytoplasmic sperm injection (ICSI). The resulting embryos were biopsied on the third day of development and the genetic analysis was performed on the same day. Embryo transfers were carried out on the fourth day.


RESULTS
The 416 stimulation cycles started yielded 280 oocyte pick-ups, 3506 oocytes retrieved, of which 2966 were suitable for ICSI. Among the 1982 embryos obtained, 1337 embryos were biopsied and genetic diagnosis was performed for 1083 (81%) of them. 381 embryos were transferred during the course of 189 transfer procedures. There were 51 clinical and 46 ongoing (35 single, 11 twin) pregnancies. In addition, 25 frozen embryo replacement cycles were initiated, leading to 6 embryo transfers and 1 ongoing pregnancy. A total of 58 unaffected children were born.


CONCLUSIONS
PGD has gained a place among the choices offered to couples at risk of transmission of a serious and incurable genetic disease. It might be a realistic alternative to prenatal diagnosis for patients carrier of chromosomal rearrangements, single gene defects, X-linked disesases or mitochondrial DNA disorders.
Feyereisen
E
E
Service de Gynécologie-Obstétrique et de Médecine de la Reproduction, Hôpital Antoine-Béclère, 157, rue de la Porte-de-Trivaux, 92141 Clamart Cedex.
Romana
S
S
Kerbrat
V
V
Steffann
J
J
Gigarel
N
N
Lelorc'h
M
M
Burlet
P
P
Ray
P
P
Hamamah
S
S
Chevalier
N
N
Fanchin
R
R
Foix-L'hélias
L
L
Tachdjian
G
G
Munnich
A
A
Frydman
R
R
Vekemans
M
M
Frydman
N
N
D000328:Adult; D020732:Cytogenetic Analysis; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D005817:Genetic Counseling; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis / Q000379:methods*; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods