Functional recovery of the striatal cholinergic system in aged rats by adenoviral vector-mediated gene transfer of dopamine D2 receptor.
We previously reported that the striatal dopamine-acetylcholine (ACh) interaction was affected by the aging process, possibly via a decrease of the striatal D(2)R expression. In the current study, the ACh responses to the infusion of 0.1microM of the D(2)R agonist quinpirole were measured with microdialysis techniques after adenoviral vector-mediated gene transfer of D(2)R into the striatum of 25-month-old rats. The D(2)R gene-transferred rats showed significantly stronger responses of the striatal cholinergic neurons to the infusion of the D(2)R agonist than did control vector-transferred rats. The current study suggests that age-associated functional decline with decreased gene expression of the receptor may be restored by intervention.
Umegaki
Hiroyuki
H
Department of Geriatrics, Nagoya University Graduate School of Medicine, 65 Tsuruma-Cho, Showa-Ku, Nagoya, Aichi 466-8550, Japan.
Yamaguchi
Yoko
Y
Ishiwata
Kiichi
K
Ingram
Donald K
DK
Roth
George S
GS
Iguchi
Akihisa
A
D000109:Acetylcholine / Q000378:metabolism; D000256:Adenoviridae / Q000235:genetics*; D000375:Aging; D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism*; D003587:Cytomegalovirus / Q000235:genetics; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D009474:Neurons / Q000378:metabolism; D019257:Quinpirole / Q000494:pharmacology; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000235:genetics*
D011950:Receptors, Cholinergic; D017448:Receptors, Dopamine D2; D019257:Quinpirole; D000109:Acetylcholine