Detection and identification of glycoyessotoxin A in a culture of the dinoflagellate Protoceratium reticulatum.
The toxin composition of a culture of the dinoflagellate Protoceratium reticulatum was investigated using LC-FLD, after derivatization with DMEQ-TAD (4-(2-(6,7-dimethoxy-4-methyl-3-oxo-3,4-dihydroquinoxalimylethyl)-1,2,4-triazoline-3,5-dione)). Besides yessotoxin (YTX), the new YTX analogue, glycoyessotoxin A (G-YTXA) was detected in culture medium as well as in cells. The conditions for extraction were optimized and the production profile established. Retention time of the resulting fluorescent G-YTXA adduct was identified by comparison of the appropriate standard. Additionally, both G-YTXA and the DMEQ-TAD-G-YTXA adduct were confirmed by LC-MS showing ion peaks at m/z 1273 [M-2Na+H](-) and m/z 1618 [M-2Na+H](-), respectively. The LC-MS(n) displayed a fragmentation pattern similar to that of the YTX series.
Paz
Beatriz
B
Unidad Asociada (CSIC-IEO) Fitoplancton Tóxico Instituto Español de Oceanografía, Centro Oceanográfico de Vigo (IEO), Apdo. 1552, 36200 Vigo, Spain.
Riobó
Pilar
P
Souto
María L
ML
Gil
Laura V
LV
Norte
Manuel
M
Fernández
José J
JJ
Franco
José M
JM
D000818:Animals; D018929:Cell Culture Techniques; D002853:Chromatography, Liquid / Q000379:methods; D004141:Dinoflagellida / Q000737:chemistry*; D004988:Ethers, Cyclic / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D008387:Marine Toxins; D013058:Mass Spectrometry; D035964:Oxocins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
D004988:Ethers, Cyclic; D008387:Marine Toxins; D035964:Oxocins; C499301:glycoyessotoxin