Lateral spread of an amplification signal using an enzymatic system on a conductive surface.
In this letter, we report a novel signal amplification phenomenon that rapidly and dramatically increases both the magnitude and the lateral extent of the original signal. This phenomenon utilizes an enzyme immobilized on a conductive surface to generate amplified signals at locations remote from the original site of enzyme activity. The result is demonstrated on a microfluidic platform using the established precipitating enzyme-substrate system of horseradish peroxidase (HRP) and 3,3',5,5'-tetramethylbenzidine (TMB) on a surface plasmon resonance (SPR) imaging system.
Hasenbank
Melissa S
MS
University of Washington, Department of Bioengineering, Box 355061, Seattle, Washington 98195, USA. hasenm@u.washington.edu
Fu
Elain
E
Yager
Paul
P
D004553:Electric Conductivity; D006735:Horseradish Peroxidase / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D006735:Horseradish Peroxidase