Mitochondrial heat shock protein 60 is increased in cerebrospinal fluid following pediatric traumatic brain injury.
Mitochondrial dysfunction occurs after traumatic brain injury (TBI) and contributes significantly to subsequent cell death. Heat shock protein 60 (hsp60) is a predominantly mitochondrial protein with important homeostatic functions. Induction of hsp60 has been demonstrated in cerebral ischemia models, possibly reflecting mitochondrial stress. We measured hsp60 concentration in the cerebrospinal fluid (CSF) of 34 infants and children after severe TBI and of 7 control patients by ELISA. Peak CSF hsp60 concentration was increased in TBI patients versus controls (0.84 ng/ml, range 0-44.59, vs. 0.0 ng/ml, range 0-0.48; p<0.05). Induction of hsp60 occurred early after the injury. Peak hsp60 concentration was independently associated with the severity of injury, defined as the admission Glasgow Coma Scale score. These data suggest that increased hsp60 in CSF might reflect the severity of early mitochondrial stress or damage after TBI.
Lai
Yichen
Y
Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, and Children's Hospital of Pittsburgh, PA 15213, USA.
Stange
Christopher
C
Wisniewski
Stephen R
SR
Adelson
P David
PD
Janesko-Feldman
Keri L
KL
Brown
Danielle S
DS
Kochanek
Patrick M
PM
Clark
Robert S B
RS
D015415:Biomarkers / Q000032:analysis / Q000134:cerebrospinal fluid; D001921:Brain / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D001930:Brain Injuries / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000503:physiopathology; D018834:Chaperonin 60 / Q000032:analysis / Q000134:cerebrospinal fluid*; D002648:Child; D002675:Child, Preschool; D004734:Energy Metabolism / Q000502:physiology; D005260:Female; D015600:Glasgow Coma Scale; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D028361:Mitochondrial Diseases / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000503:physiopathology; D011237:Predictive Value of Tests; D011379:Prognosis; D013312:Stress, Physiological / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D015854:Up-Regulation / Q000502:physiology
D015415:Biomarkers; D018834:Chaperonin 60