Archaea predominate among ammonia-oxidizing prokaryotes in soils.
Ammonia oxidation is the first step in nitrification, a key process in the global nitrogen cycle that results in the formation of nitrate through microbial activity. The increase in nitrate availability in soils is important for plant nutrition, but it also has considerable impact on groundwater pollution owing to leaching. Here we show that archaeal ammonia oxidizers are more abundant in soils than their well-known bacterial counterparts. We investigated the abundance of the gene encoding a subunit of the key enzyme ammonia monooxygenase (amoA) in 12 pristine and agricultural soils of three climatic zones. amoA gene copies of Crenarchaeota (Archaea) were up to 3,000-fold more abundant than bacterial amoA genes. High amounts of crenarchaeota-specific lipids, including crenarchaeol, correlated with the abundance of archaeal amoA gene copies. Furthermore, reverse transcription quantitative PCR studies and complementary DNA analysis using novel cloning-independent pyrosequencing technology demonstrated the activity of the archaea in situ and supported the numerical dominance of archaeal over bacterial ammonia oxidizers. Our results indicate that crenarchaeota may be the most abundant ammonia-oxidizing organisms in soil ecosystems on Earth.
Leininger
S
S
Department of Biology, University of Bergen, Jahnebakken 5, N-5020 Bergen, Norway.
Urich
T
T
Schloter
M
M
Schwark
L
L
Qi
J
J
Nicol
G W
GW
Prosser
J I
JI
Schuster
S C
SC
Schleper
C
C
D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D017753:Ecosystem; D018628:Gene Dosage / Q000235:genetics; D015723:Gene Library; D019766:Genes, Archaeal / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D020459:Genes, rRNA / Q000235:genetics; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D011387:Prokaryotic Cells / Q000378:metabolism*; D019642:RNA, Archaeal / Q000032:analysis / Q000235:genetics; D012988:Soil Microbiology
D018076:DNA, Complementary; D008055:Lipids; D019642:RNA, Archaeal; D000641:Ammonia; D010088:Oxidoreductases; C045582:ammonia monooxygenase