Mining frequent patterns for AMP-activated protein kinase regulation on skeletal muscle.
BACKGROUND
AMP-activated protein kinase (AMPK) has emerged as a significant signaling intermediary that regulates metabolisms in response to energy demand and supply. An investigation into the degree of activation and deactivation of AMPK subunits under exercise can provide valuable data for understanding AMPK. In particular, the effect of AMPK on muscle cellular energy status makes this protein a promising pharmacological target for disease treatment. As more AMPK regulation data are accumulated, data mining techniques can play an important role in identifying frequent patterns in the data. Association rule mining, which is commonly used in market basket analysis, can be applied to AMPK regulation.


RESULTS
This paper proposes a framework that can identify the potential correlation, either between the state of isoforms of alpha, beta and gamma subunits of AMPK, or between stimulus factors and the state of isoforms. Our approach is to apply item constraints in the closed interpretation to the itemset generation so that a threshold is specified in terms of the amount of results, rather than a fixed threshold value for all itemsets of all sizes. The derived rules from experiments are roughly analyzed. It is found that most of the extracted association rules have biological meaning and some of them were previously unknown. They indicate direction for further research.


CONCLUSIONS
Our findings indicate that AMPK has a great impact on most metabolic actions that are related to energy demand and supply. Those actions are adjusted via its subunit isoforms under specific physical training. Thus, there are strong co-relationships between AMPK subunit isoforms and exercises. Furthermore, the subunit isoforms are correlated with each other in some cases. The methods developed here could be used when predicting these essential relationships and enable an understanding of the functions and metabolic pathways regarding AMPK.
Chen
Qingfeng
Q
School of Engineering & Information Technology, Deakin University, Melbourne, Australia. qifengch@deakin.edu.au
Chen
Yi-Ping Phoebe
YP
D055372:AMP-Activated Protein Kinases; D000465:Algorithms; D003920:Diabetes Mellitus / Q000378:metabolism; D004734:Energy Metabolism; D015444:Exercise; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008432:Mathematical Computing; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D021122:Protein Subunits; D015398:Signal Transduction; D012984:Software
D007527:Isoenzymes; D009097:Multienzyme Complexes; D021122:Protein Subunits; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases