title: Protective effect of irradiated renal carcinoma expressing hepatitis B surface antigen against renal-cell carcinoma-mediated tumors.
abstract: Radiation therapy and chemotherapy have little effect on renal-cell carcinomas (RENCAs). We investigated the effect of the tumor vaccination strategy on preventing tumor formation after a challenge with RENCA. The hepatitis surface antigen (HBsAg) was used to enhance the antitumor immunity and tumor vaccination efficiency. RENCA cells expressing HBsAg (RENCA/HBS) were completely susceptible to HBsAg vaccination, which implies that HBsAg vaccination induces specific antitumor immunity against HBsAg- expressing cancer cells. As with HBsAg vaccination, vaccination with irradiated RENCA/HBS retarded tumor formation following a RENCA/HBS challenge. After HBsAg vaccination, the irradiated RENCA/HBS tumor vaccine completely prevented the tumor formation by RENCA/HBS. Tumor vaccination with irradiated RENCA/HBS (5 x 10(4) cells), but not with RENCA, reduced the tumor rate after a challenge with 5 x 10(6) RENCA cells, whereas a lower tumor load was overcome by the RENCA vaccination alone. These results confirm the postulate that RENCA/HBS vaccination elicits an antitumor immune response to some putative antigens or enhances the general immune competence in immunosuppressed renal tumor patients.
authors.affiliation: Department of Microbiology and Immunology, Medical Research Institute, Pusan National University Medical School, Busan, Korea.
authors.forename: Yuseok
authors.initials: Y
authors.lastname: Moon
authors.forename: Seok Goo
authors.initials: SG
authors.lastname: Cho
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Lee
authors.forename: Woo Sung
authors.initials: WS
authors.lastname: Min
authors.forename: Chun Choo
authors.initials: CC
authors.lastname: Kim
authors.forename: Hae-Kyung
authors.initials: HK
authors.lastname: Lee
authors.forename: Yong-Goo
authors.initials: YG
authors.lastname: Kim
authors.forename: Hong Seok
authors.initials: HS
authors.lastname: Chang
authors.forename: Hyun Seok
authors.initials: HS
authors.lastname: Chae
authors.forename: Ho-Youn
authors.initials: HY
authors.lastname: Kim
authors.forename: Kenzaburo
authors.initials: K
authors.lastname: Tani
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000378:metabolism / Q000627:therapeutic use*; D002292:Carcinoma, Renal Cell / Q000532:radiotherapy* / Q000628:therapy; D045744:Cell Line, Tumor; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007680:Kidney Neoplasms / Q000532:radiotherapy* / Q000628:therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism
chemical_list: D019496:Cancer Vaccines; D006514:Hepatitis B Surface Antigens; D007166:Immunosuppressive Agents