title: [West Nile virus genotyping among wild birds belonging to ground and tree-brush bird populations on the territories of the Baraba forest-steppe and Kulunda steppe (2003-2004)].
abstract: The paper gives the results of the 2003-2004 examinations of 104 wild birds belonging to land tree-brush complexes from the Baraba forest-steppe and Kulunda steppe for the detection and genotyping West Nile virus (WNV). ELISA and RT-PCR were used to show that in the forest-steppe and steppe zones of the south of Western Siberia, WNV circulates among both migrating and settled birds. An analysis of the nucleotide sequence of a protein E gene fragment showed the circulation of WNV genotype Ia in the study birds. A number of revealed amino acid substitutions in surface glycoprotein E are unique for the 2003-2004 Western-Siberian WNV variants and absent in the 2002 Western-Siberian variants, which suggests that there are regional features of the evolution of WNV genotype Ia.
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Kononova
authors.forename: V A
authors.initials: VA
authors.lastname: Ternovoĭ
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Shchelkanov
authors.forename: E V
authors.initials: EV
authors.lastname: Protopopova
authors.forename: S I
authors.initials: SI
authors.lastname: Zolotykh
authors.forename: A K
authors.initials: AK
authors.lastname: Iurlov
authors.forename: A V
authors.initials: AV
authors.lastname: Druziaka
authors.forename: A A
authors.initials: AA
authors.lastname: Slavskiĭ
authors.forename: A M
authors.initials: AM
authors.lastname: Shestopalov
authors.forename: D K
authors.initials: DK
authors.lastname: L'vov
authors.forename: V B
authors.initials: VB
authors.lastname: Loktev
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild / Q000145:classification / Q000821:virology*; D000956:Antigens, Viral / Q000235:genetics; D001717:Birds / Q000821:virology*; D017753:Ecosystem; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D012800:Siberia; D013045:Species Specificity; D014197:Trees; D014759:Viral Envelope Proteins / Q000235:genetics; D014902:West Nile virus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000956:Antigens, Viral; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus