title: Reduction of chromate by cell-free extract of Brucella sp. isolated from Cr(VI) contaminated sites.
abstract: A locally isolated gram negative strain of Brucella sp., identified by biochemical methods and 16SrRNA analysis, reduced chromate to 100%, 94.1%, 93.2%, 66.9% and 41.6% at concentrations of 50, 100, 150, 200 and 300mgl(-1), respectively at pH 7 and temperature 37 degrees C. Increasing concentrations of Cr(VI) in the medium lowered the growth rate but could not be directly correlated with the amount of Cr(VI) reduced. The strain also exhibited multiple heavy metal (Ni,Zn,Hg,Pb,Co) tolerance and resistance to various antibiotics. Assay with crude cell-free extracts demonstrated that the hexavalent chromium reduction was mainly associated with the soluble fraction of the cell. High Cr(VI) concentration resistance and high Cr(VI) reducing ability of the strain make it a suitable candidate for bioremediation.
authors.affiliation: Post Graduate Department of Biosciences, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat, India.
authors.forename: Urvashi
authors.initials: U
authors.lastname: Thacker
authors.forename: Rasesh
authors.initials: R
authors.lastname: Parikh
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Shouche
authors.forename: Datta
authors.initials: D
authors.lastname: Madamwar
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D002002:Brucella / Q000235:genetics / Q000378:metabolism*; D002840:Chromates / Q000378:metabolism*; D016000:Cluster Analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants / Q000378:metabolism*; D013053:Spectrophotometry; D013696:Temperature
chemical_list: D002840:Chromates; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants