title: Effects of static and 50 Hz alternating electric fields on superoxide dismutase activity and TBARS levels in guinea pigs.
abstract: The toxic oxygen free radicals are extremely reactive and can cause considerable damage to biomolecules, such as RNA, enzymes, membranes, proteins, and lipids, which may in turn lead to various pathological consequences. Lipid peroxidation, evaluated by determination of thiobarbituric acid reactive substances (TBARS) is the free radical-induced oxidation of polyunsaturated fatty acids. Normally, the oxygen free radicals are neutralized by highly efficient systems in the body. These include antioxidant enzymes like superoxide dismutase (SOD). In a healthy subject, there is a balance between free radicals and levels of antioxidants. The aim of this study was to determine lipid peroxidation and SOD levels in plasma, liver, lung and kidney tissues exposed to different intensities, directions and exposure periods of static and 50 Hz alternating electric fields. Electric field intensities ranging from 0.3 kV/m to 1.8 kV/m were applied in vertical or horizontal direction in exposure periods of 1, 3, 5, 7, and 10 days. The increase in SOD and TBARS levels of plasma, liver, lung, and kidney tissues was found to depend significantly on the type of electric field and the exposure period.
authors.affiliation: Department of Biophysics, Medical Faculty, Gazi University, Beşevler, Ankara, Turkey. gozturk@gazi.edu.tr
authors.forename: G
authors.initials: G
authors.lastname: Güler
authors.forename: N
authors.initials: N
authors.lastname: Seyhan
authors.forename: A
authors.initials: A
authors.lastname: Aricioğlu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004574:Electromagnetic Fields; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D006168:Guinea Pigs; D015227:Lipid Peroxidation; D008055:Lipids / Q000737:chemistry; D008297:Male; D010100:Oxygen / Q000378:metabolism; D012313:RNA / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D000975:Antioxidants; D005231:Fatty Acids, Unsaturated; D005609:Free Radicals; D008055:Lipids; D017392:Thiobarbituric Acid Reactive Substances; D012313:RNA; D013482:Superoxide Dismutase; D010100:Oxygen