title: P-glycoprotein effect on the properties of its natural lipid environment probed by Raman spectroscopy and Langmuir-Blodgett technique.
abstract: Behavior of P-glycoprotein (Pgp) natural lipid environment within the membrane of CEM cells expressing Pgp in the quantities varying from 0% to 32% of the total amount of all membrane proteins is described for the first time. Observed cooperative effect of Pgp-induced increase of membrane stability, decrease of the temperature of gel-to-crystal lipids transition and predominance of the lipid liquid crystalline phase at physiological temperatures should have an impact in development of multidrug resistance phenotype of tumor cells by favoring the Pgp intercellular transfer and Pgp ATPase activity.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. Miklukho-Maklaya, 117997 Moscow, Russia. voleinik@mail.ru
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Oleinikov
authors.forename: Fabrice
authors.initials: F
authors.lastname: Fleury
authors.forename: Anatoli
authors.initials: A
authors.lastname: Ianoul
authors.forename: Sergey
authors.initials: S
authors.lastname: Zaitsev
authors.forename: Igor
authors.initials: I
authors.lastname: Nabiev
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D013696:Temperature
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008055:Lipids