Detection of egg yolk antibodies reflecting Salmonella enteritidis infections using a surface plasmon resonance biosensor.
A surface plasmon resonance (SPR) biosensor assay was developed on the basis of a lipopolysaccharide antigen of Salmonella enterica serovar enteritidis (S. enterica serovar enteritidis) to detect egg yolk antibodies against S. enterica serovar enteritidis. This biosensor assay was compared to two commercial ELISA kits based on LPS antigen and flagellar antigen. A number of 163 egg yolk and combined egg white and yolk samples from chickens experimentally infected with S. enterica serovar enteritidis and 90 egg yolk and combined egg white and yolk samples from uninfected chickens were analyzed. Receiver operating characteristic analysis of the data calculated a diagnostic sensitivity of 82% and a diagnostic specificity of 100%. The within-day coefficient of variation of a positive internal-control egg yolk was 1%. The SPR biosensor assay was able to detect antibodies in a significantly higher percentage of known positive samples than the commercial ELISA's. The anticipated use of the SPR biosensor assay is to determine the S. enterica serovar enteritidis serostatus of non-vaccinated layer hens.
Thomas
Ekelijn
E
Public Health and Food Safety Division, Institute for Risk Assessment Sciences, Utrecht University, Yalelaan 2, 3584 CM Utrecht, The Netherlands.
Bouma
Annemarie
A
van Eerden
Ellen
E
Landman
Wil J M
WJ
van Knapen
Frans
F
Stegeman
Arjan
A
Bergwerff
Aldert A
AA
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology*; D015374:Biosensing Techniques / Q000379:methods*; D002645:Chickens / Q000382:microbiology*; D004530:Egg Yolk / Q000276:immunology* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005408:Flagellin / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides; D005408:Flagellin