Molecular characterization of caprine adeno-associated virus (AAV-Go.1) reveals striking similarity to human AAV5.
The complete genome of the caprine adeno-associated virus (AAV-Go.1) was sequenced, and the expression profile of AAV-Go.1 was determined following virus infection in primary lamb kidney cells. A remarkable similarity between the Rep coding region, the ITR sequence and the central intron of AAV-Go.1, and the human derived AAV5 was observed. The transcription profile of AAV-Go.1 was also quite similar to that of AAV5. AAV-Go.1 was able to efficiently infect human cell lines, following co-infection of human adenovirus, and in reciprocal experiments, AAV5 also was able to efficiently infect primary lamb cells and bovine cell lines. Recombinant AAV5 and AAV-Go.1 expressing the beta-gal gene flanked by AAV5 ITRs, showed similar transduction efficiency in various human and animal cells. Studies of AAV-Go.1 may expand our understanding of the evolutionary relationship between the AAV-5-like group of AAVs, which, in addition to human derived AAV5, includes AAV viruses from bovine, caprine, and avian species.
Qiu
Jianming
J
Department of Molecular Microbiology and Immunology, Life Sciences Center, University of Missouri-Columbia, School of Medicine, Columbia, MO 65211, USA. QiuJ@missouri.edu <QiuJ@missouri.edu>
Cheng
Fang
F
Pintel
David
D
D000818:Animals; D019556:COS Cells; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D000229:Dependovirus / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D016679:Genome, Viral; D006041:Goats / Q000821:virology*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D001616:beta-Galactosidase