title: Changes in functional properties of the caffeine-sensitive Ca2+ store during differentiation of human SH-SY5Y neuroblastoma cells.
abstract: We have used single cell fluorescence imaging techniques to examine how functional properties of the caffeine-sensitive Ca(2+) store change during differentiation of a sub-population of caffeine-sensitive SH-SY5Y cells. Application of caffeine (30 mM) 1-10.5 min after a 'priming' depolarisation pulse of 55 mM K(+) revealed that the caffeine-sensitive store in undifferentiated cells remained replete, whereas that in 9-cis retinoic acid (9cRA)-differentiated cells spontaneously dissipated with a t(1/2) of 2.8 min, and was essentially completely depleted approximately 10 min after priming. In 9cRA-differentiated cells that were stimulated with methacholine (10 microM) 1 min after priming, the amplitude, rate of rise and propagation velocity of the Ca(2+) wave in the neurites were all constant, whereas these kinetic parameters all progressively decreased as the wave travelled along the neurites in cells that were stimulated 10 min after priming. Use-dependent block with ryanodine inhibited the global Ca(2+) signal in 9cRA-differentiated cells stimulated with methacholine 1 min after priming (71+/-8%) but not 10 min after priming. Depolarisation was more effective at priming the caffeine-sensitive Ca(2+) store in 9cRA-differentiated cells, which lack a functional store-operated Ca(2+) entry pathway. We conclude that differentiation of caffeine-sensitive SH-SY5Y cells is accompanied by an increase in lability of the caffeine-sensitive Ca(2+) store, and that spontaneous dissipation of Ca(2+) from the store limits the time course of its molecular 'memory' during which it can amplify the hormone-induced Ca(2+) signal by Ca(2+)-induced Ca(2+) release.
authors.affiliation: Institute for Cell & Molecular Biosciences, The Medical School, University of Newcastle-Upon-Tyne, Framlington Place, Newcastle-Upon-Tyne NE2 4HH, UK.
authors.forename: Fiona C
authors.initials: FC
authors.lastname: Riddoch
authors.forename: Anna M
authors.initials: AM
authors.lastname: Brown
authors.forename: Sophie E
authors.initials: SE
authors.lastname: Rowbotham
authors.forename: Christopher P F
authors.initials: CP
authors.lastname: Redfern
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Cheek
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D016210:Methacholine Chloride / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D016501:Neurites / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism*; D010277:Parasympathomimetics / Q000494:pharmacology; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D010277:Parasympathomimetics; D010726:Phosphodiesterase Inhibitors; D016210:Methacholine Chloride; D002110:Caffeine; D014212:Tretinoin; D011188:Potassium; D002118:Calcium