Cloning and expression of ARMC3_v2, a novel splicing variant of the human ARMC3 gene.
ARM genes, whose polypeptide consist of Armadillo/beta-catenin-like repeats (ARM) domain(s), exist ubiquitously from fly to vertebrates. These genes have multiple functions in signal transduction, development, cell adhesion and mobility, tumor initiation and metastasis. In this study, we have isolated a novel splicing variant of ARMC3 from human fetal brain, which is 2439 bp, encoding a 688-amino acid polypeptide that contains three typical ARM domains. The cDNA called ARMC3_v2 and the original called ARMC3_v1 (GeneBank: BC039312) are both located on the human chromosome 10p12.23. RT-PCR analysis in our work showed that ARMC3_v2 was detected in human skeletal muscle, liver, spleen and thymus; in contrast, ARMC3_v1 in skeletal muscle, lung, prostate and testis.
Li
X
X
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, China.
Liu
B
B
Ji
C N
CN
Kang
Y
Y
Mao
Y
Y
D000595:Amino Acid Sequence; D051186:Armadillo Domain Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D003001:Cloning, Molecular; D005333:Fetus; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity; D017434:Protein Structure, Tertiary; D012326:RNA Splicing
C513647:ARMC3 protein, human; D051186:Armadillo Domain Proteins