title: Copper-dependent co-internalization of the prion protein and glypican-1.
abstract: Heparan sulfate chains have been found to be associated with amyloid deposits in a number of diseases including transmissible spongiform encephalopathies. Diverse lines of evidence have linked proteoglycans and their glycosaminoglycan chains, and especially heparan sulfate, to the metabolism of the prion protein isoforms. Glypicans are a family of glycosylphosphatidylinositol-anchored, heparan sulfate-containing, cell-associated proteoglycans. Cysteines in glypican-1 can become nitrosylated by endogenously produced nitric oxide. When glypican-1 is exposed to a reducing agent, such as ascorbate, nitric oxide is released and autocatalyses deaminative cleavage of heparan sulfate chains. These processes take place while glypican-1 recycles via a non-classical, caveolin-associated pathway. We have previously demonstrated that prion protein provides the Cu2+ ions required to nitrosylate thiol groups in the core protein of glypican-1. By using confocal immunofluorescence microscopy and immunomagnetic techniques, we now show that copper induces co-internalization of prion protein and glypican-1 from the cell surface to perinuclear compartments. We find that prion protein is controlling both the internalization of glypican-1 and its nitric oxide-dependent autoprocessing. Silencing glypican-1 expression has no effect on copper-stimulated prion protein endocytosis, but in cells expressing a prion protein construct lacking the copper binding domain internalization of glypican-1 is much reduced and autoprocessing is abrogated. We also demonstrate that heparan sulfate chains of glypican-1 are poorly degraded in prion null fibroblasts. The addition of either Cu2+ ions, nitric oxide donors, ascorbate or ectopic expression of prion protein restores heparan sulfate degradation. These results indicate that the interaction between glypican-1 and Cu2+-loaded prion protein is required both for co-internalization and glypican-1 self-pruning.
authors.affiliation: Department of Experimental Medical Science, Division of Neuroscience, Glycobiology Group, Lund University, Lund, Sweden.
authors.forename: Fang
authors.initials: F
authors.lastname: Cheng
authors.forename: Josefin
authors.initials: J
authors.lastname: Lindqvist
authors.forename: Cathryn L
authors.initials: CL
authors.lastname: Haigh
authors.forename: David R
authors.initials: DR
authors.lastname: Brown
authors.forename: Katrin
authors.initials: K
authors.lastname: Mani
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D051242:Caveolin 1 / Q000032:analysis; D045744:Cell Line, Tumor; D003300:Copper / Q000502:physiology*; D003600:Cytosol / Q000187:drug effects / Q000502:physiology; D005455:Fluorescent Antibody Technique / Q000379:methods; D049452:Green Fluorescent Proteins / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000494:pharmacology; D018189:Immunomagnetic Separation / Q000379:methods; D047468:Immunoprecipitation / Q000379:methods; D051379:Mice; D009447:Neuroblastoma; D009569:Nitric Oxide / Q000494:pharmacology; D011328:Prions / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects / Q000502:physiology; D034741:RNA, Small Interfering / Q000494:pharmacology; D014162:Transfection / Q000379:methods
chemical_list: D051242:Caveolin 1; D003300:Copper; D049452:Green Fluorescent Proteins; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D009569:Nitric Oxide; D011328:Prions; D034741:RNA, Small Interfering