A nanophosphor-based method for selective DNA recovery in Synthosomes.
A nanocompartment system composed of an ABA triblock copolymer, where A is poly(dimethylsiloxane) and B is poly(2-methyloxazoline), has been developed for selective recovery and detection of DNA. Translocation of TAMRA-labeled complementary primers into the nanocompartment system has been achieved through two deletion mutants (FhuA Delta1-129; FhuA Delta1-160) of the channel protein FhuA. Translocation was monitored by fluorescence resonance energy transfer through hybridization of the TAMRA-labeled primer to the complementary sequence of a nanophosphor-DNA-conjugate, which reduces its half-life (FhuA Delta1-129, 16.0% reduced; FhuA Delta1-160, 39.0% reduced).
Nallani
Madhavan
M
International University Bremen, School of Engineering and Science, Bremen, Germany.
Onaca
Ozana
O
Gera
Nimish
N
Hildenbrand
Karlheinz
K
Hoheisel
Werner
W
Schwaneberg
Ulrich
U
D001672:Biocompatible Materials / Q000737:chemistry*; D004247:DNA / Q000302:isolation & purification*; D008081:Liposomes / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D001672:Biocompatible Materials; D008081:Liposomes; D004247:DNA