Development of a gradient reversed-phase HPLC method for the determination of sodium ferulate in beagle dog plasma.
A gradient reversed-phase HPLC assay has been developed to determine sodium ferulate (SF) in beagle dog plasma with tinidazole as an internal standard. Chromatographic separation was made on a C(18) column using 0.5% acetic acid and acetonitrile (80:20, v/v) as mobile phase. UV detection was performed at 320 nm. The calibration curve for SF was linear in the range of 0.05-10 microg/ml, and the achieved limit of quantification (LOQ) was 51.4 ng/ml. The results of linearity, within- and between-day precision, and accuracy demonstrate that this method is reliable, sensitive and sufficient for in vivo beagle dog pharmacokinetic (PK) studies of SF.
Li
Feng-Qian
FQ
Department of Pharmaceutical Sciences, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Xu
Shu
S
Su
Hua
H
Deng
Jia-Xin
JX
Liu
Ji-Yong
JY
Hu
Jin-Hong
JH
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000097:blood* / Q000493:pharmacokinetics; D004285:Dogs; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003373:Coumaric Acids; C004999:ferulic acid