In vitro microspore selection in maize anther culture with oxidative-stress stimulators.
In order to produce doubled-haploid maize plants tolerant of oxidative stress, in vitro microspore selection was carried out in anther culture with reactive oxygen species (ROS) progenitors such as paraquat, menadione, tert-butylhydroperoxide (t-BHP), and methionine combined with riboflavin. All the ROS progenitors reduced the anther induction, the formation of microspore-derived structures, and their regeneration potential. Abnormal cell divisions and progeny cell degradation could be observed during the development of microspores treated with ROS progenitors. Menadione and t-BHP influenced the microspore developmental pathway, as menadione induced the formation of embryoids, while t-BHP increased the proportion of calli in the microspore-derived structures. As the result of in vitro selection, 15, 10, 10, and 3 fertile doubled-haploid plants were obtained in cultures treated with paraquat, t-BHP, methionine combined with riboflavin, and menadione, respectively.
Ambrus
H
H
Agricultural Research Institute, Hungarian Academy of Sciences, Martonvásár, Hungary.
Darko
E
E
Szabo
L
L
Bakos
F
F
Király
Z
Z
Barnabás
B
B
D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D035264:Flowers / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012038:Regeneration / Q000187:drug effects; D012639:Seeds / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D003313:Zea mays / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D017382:Reactive Oxygen Species