Detection and quantitation of circulating Plasmodium falciparum DNA by polymerase chain reaction.
This chapter describes the application of polymerase chain reaction (PCR) for the detection and quantitation of Plasmodium falciparum DNA in the plasma of malaria-infected individuals. The procedure includes the following protocols: plasma sample preparation, DNA extraction, detection of P. falciparum DNA in the plasma by nested PCR, and quantitation of P. falciparum DNA in the plasma by real-time PCR technology.
Gal
Shira
S
Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK.
Wainscoat
James S
JS
D000818:Animals; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008288:Malaria / Q000097:blood* / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D004247:DNA