The cytokine response of circulating peripheral blood mononuclear cells is changed after intravenous injection of lipopolysaccharide in cattle.
The aim of the study was to investigate lipopolysaccharide (LPS)-induced short and long term changes in capacity for intracellular cytokine-production of bovine circulating peripheral blood mononuclear cells (PBMCs). Eight dairy cows each received three intravenous injections of Escherichia coli LPS (10, 100 and 1000ng/kg, consecutively) at 3week intervals. Intracellular cytokine production was determined by flow cytometry in PBMCs obtained 0, 2, 6 and 24h after each LPS challenge. After LPS administration, proportions of monocytes producing tumour necrosis factor (TNF) alpha, interleukin (IL)-1beta and IL-8, as well as proportions of circulating lymphocytes producing interferon (IFN) gamma, decreased significantly. Within 24h, proportions had returned to or increased above pre-injection levels. Proportions of lymphocytes producing IL-4 and IL-10 increased significantly after injection of 1000ng LPS/kg. This study demonstrated that cytokine profiles shift quickly, but temporarily, to favour the anti-inflammatory response immediately after LPS exposure. The long term response to LPS was opposite to the immediate response, as cytokine profiles shifted in the 3weeks between challenges towards a pro-inflammatory response. Proportions of monocytes producing IL-1beta and TNFalpha determined immediately before the second and/or third LPS injection were higher than proportions determined before the first injection, whereas pre-injection proportions of lymphocytes producing IL-4 decreased with each challenge. These changes may result in a quicker host response to invading pathogens.
Jacobsen
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Department of Large Animal Sciences, Large Animal Surgery, The Royal Veterinary and Agricultural University, Dyrlaegevej 48, DK-1870 Frederiksberg, C, Copenhagen, Denmark. stj@kvl.dk
Andersen
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PH
Aasted
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B
D000818:Animals; D002417:Cattle / Q000097:blood / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007378:Interleukins / Q000097:blood / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000276:immunology
D016207:Cytokines; D007378:Interleukins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha