title: [Asparagine synthetase activity in pediatric acute lymphoblastic leukemia].
abstract: OBJECTIVE
  To study the cellular activity of asparagine synthetase in different types of childhood acute lymphoblastic leukemia (ALL).
  
  
  METHODS
  The cellular activity of asparagine synthetase was detected by HPLC-FLD and Protein measurement in 28 ALL children (7 cases of T-ALL and 21 cases of B-lymphoid lineage ALL) before chemotherapy.
  
  
  RESULTS
  The asparagines synthetase activity levels in T-ALL children were significantly higher than those of the B-lymphoid lineage ALL patients, with the median activity level of 9.3 nM Asn/mg protein/hr vs 5.2 nM Asn/mg protein/hr (P < 0.05). The distribution of the asparagine synthetase activity demonstrated a polymorphism in either T-ALL or B-lymphoid lineage ALL patients.
  
  
  CONCLUSIONS
  The cellular activity of asparagines synthetase in ALL patients is presented with a polymorphism distribution. The asparagines synthetase activity levels in T-ALL are significantly higher than in B-lymphoid lineage ALL.
authors.affiliation: Department of Hematology/Oncology, Shanghai Children's Medical Center Affiliated to Medical College of Shanghai Jiaotong University, Shanghai 200127, China. jiang_hua18@yahoo.com.cn
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Long-Jun
authors.initials: LJ
authors.lastname: Gu
authors.forename: Hui-Liang
authors.initials: HL
authors.lastname: Xue
authors.forename: Jing-Yan
authors.initials: JY
authors.lastname: Tang
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Ci
authors.initials: C
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
mesh_terms: D001215:Asparaginase / Q000627:therapeutic use; D001217:Aspartate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism*; D002648:Child; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D001215:Asparaginase; D001217:Aspartate-Ammonia Ligase; D050676:Core Binding Factor Alpha 2 Subunit; D015514:Oncogene Proteins, Fusion; D012333:RNA, Messenger; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein