Biofilm fermentation of iturin A by a recombinant strain of Bacillus subtilis 168.
Bacillus subtilis 168 produces thin and fragile biofilm in the static culture, however, it was found out that its transformant B. subtilis RM/iSd16 containing wild sfp, itu operon and degQ, which produced lipopeptide antibiotic iturin A, produced thick and much stable biofilm. Production of iturin A by RM/iSd16 in biofilm was almost two times higher compared to that in the submerged culture at 28 degrees C.
Rahman
Mohammad Shahedur
MS
Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan. rahman@res.titech.ac.jp
Ano
Takashi
T
Shoda
Makoto
M
D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development*; D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms / Q000254:growth & development*; D009876:Operon / Q000235:genetics; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000254:growth & development; D010456:Peptides, Cyclic / Q000096:biosynthesis* / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics
D001426:Bacterial Proteins; C091639:DegQ protein, Bacillus sp.; D010456:Peptides, Cyclic; D015534:Trans-Activators; C013579:iturin A