title: Prospective use of electrospun ultra-fine silicate fibers for bone tissue engineering.
abstract: Electrospinning involves the generation of a jet of viscous solution, and results in the formation of ultra-fine fibers. Silicate fibers prepared using this technology and via the sol-gel process were evaluated as scaffolds for bone tissue engineering. We found that human osteoblastic MG63 cells successfully adhered on individual silicate fibers, and proliferated on them. In an apatite-formation ability study, spherical particles covered the fibers after soaking in simulated body fluid for 7 days. Energy dispersive X-ray analysis revealed that Ca/P atomic ratio of the particles was similar to that of human bone. In addition, X-ray diffraction analysis revealed that crystalline structure of the particles agreed with that of apatite. These results suggest that electrospun silicate fibers are a potential candidate for scaffolding material in bone tissue engineering.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Nishi-ku, Fukuoka, Japan. sakai@chem-eng.kyushu-u.ac.jp
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yamada
authors.forename: Tetsu
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Koei
authors.initials: K
authors.lastname: Kawakami
mesh_terms: D001031:Apatites / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D001826:Body Fluids; D018786:Bone Substitutes; D001842:Bone and Bones / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D006801:Humans; D010006:Osteoblasts / Q000378:metabolism; D044367:Phase Transition; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible