Inhibition of Listeria innocua growth by antimicrobial-producing lactic acid cultures in vacuum-packed cold-smoked salmon.
The biopreservative potential of three antimicrobial-producing lactic acid bacteria strains was evaluated on cold-smoked salmon. Lactobacillus casei, Lactobacillus plantarum and Carnobacterium piscicola were added singly or in association to cold-smoked salmon, artificially contaminated with Listeria innocua and stored under vacuum for 30 days at 4 degrees C. All the lactic cultures were able to inhibit Listeria innocua growth, showing a bacteriostatic or bactericidal effect, without affecting negatively the sensory quality of the product. Lactobacillus casei was bacteriostatic when inoculated at 6 log cfu/g, but bactericidal at 8 log cfu/g, reducing Listeria innocua of 3.3 log cfu/g in comparison with the test at the end of storage. Lactobacillus plantarum and C. piscicola strains, inoculated singly at 6 log cfu/g reduced Listeria innocua counts of 2.8 and 2.7 log cfu/g, respectively, compared with the test. The association Lactobacillus casei-Lactobacillus plantarum was the most effective among the treatments with 6 log cfu/g inoculum, as Listeria innocua counts decreased of 3.2 log cfu/g compared with the test. The treatment with Lactobacillus casei-C. piscicola association was less effective than C. piscicola alone.
Vescovo
Marisa
M
Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, 29100 Piacenza, Italy. marisa.vescovo@unicatt.it
Scolari
Gianluigi
G
Zacconi
Carla
C
D000818:Animals; D000898:Antibiosis; D015169:Colony Count, Microbial; D005506:Food Contamination / Q000032:analysis*; D018857:Food Packaging / Q000379:methods*; D005519:Food Preservation / Q000379:methods*; D006801:Humans; D019344:Lactic Acid / Q000096:biosynthesis / Q000494:pharmacology; D007778:Lactobacillus / Q000378:metabolism / Q000502:physiology*; D007780:Lacticaseibacillus casei / Q000378:metabolism / Q000502:physiology; D048191:Lactiplantibacillus plantarum / Q000378:metabolism / Q000502:physiology; D008087:Listeria / Q000187:drug effects / Q000254:growth & development*; D012474:Salmon / Q000382:microbiology*; D017747:Seafood / Q000382:microbiology*; D013696:Temperature; D013997:Time Factors; D014618:Vacuum
D019344:Lactic Acid