Immortalization of human myogenic progenitor cell clone retaining multipotentiality.
Human myogenic cells have limited ability to proliferate in culture. Although forced expression of telomerase can immortalize some cell types, telomerase alone delays senescence of human primary cultured myogenic cells, but fails to immortalize them. In contrast, constitutive expression of both telomerase and the E7 gene from human papillomavirus type 16 immortalizes primary human myogenic cells. We have established an immortalized primary human myogenic cell line preserving multipotentiality by ectopic expression of telomerase and E7. The immortalized human myogenic cells exhibit the phenotypic characteristics of their primary parent, including an ability to undergo myogenic, osteogenic, and adipogenic terminal differentiation under appropriate culture conditions. The immortalized cells will be useful for both basic and applied studies aimed at human muscle disorders. Furthermore, immortalization by transduction of telomerase and E7 represents a useful method by which to expand human myogenic cells in vitro without compromising their ability to differentiate.
Hashimoto
Naohiro
N
Stem Cell Research Unit, Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan. nao@nils.go.jp
Kiyono
Tohru
T
Wada
Michiko R
MR
Shimizu
Shirabe
S
Yasumoto
Shigeru
S
Inagawa
Masayo
M
D000328:Adult; D002999:Clone Cells; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D006801:Humans; D007621:Karyotyping; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000235:genetics; D050725:Papillomavirus E7 Proteins; D019098:Telomerase / Q000235:genetics
D004268:DNA-Binding Proteins; D009124:Muscle Proteins; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D019098:Telomerase