title: Influence of storage temperature on the stability of HIV-1 RNA and HSV-2 DNA in cervicovaginal secretions collected by vaginal washing.
abstract: Variability in the handling of samples of genital secretions prior to quantitation of HIV-1 RNA and HSV DNA may profoundly affect both the detection and quantitation of these nucleic acids. Over 144 h, we evaluated, the influence of storage temperature (4 degrees C, 20 degrees C, 30 degrees C) on the quantity of HIV-1 RNA and HSV-2 DNA in HIV and HSV negative cervicovaginal lavage pools spiked with known amounts of HIV-1 and HSV-2 and in HIV-1 and HSV-2 co-infected cervicovaginal lavage pools. The level of viral nucleic acids remained stable at 4 degrees C for 24h but decreased significantly when cervicovaginal lavages were stored at 20 degrees C and 30 degrees C, demonstrating that, cervicovaginal lavages to be quantified for viral RNA or DNA require, at minimum, immediate storage at 4 degrees C.
authors.affiliation: Université Paris V, Equipe Immunité et Biothérapie Muqueuses, Unité INSERM Internationale U743 Immunologie Humaine, & Laboratoire de Virologie, Hôpital Européen Georges Pompidou, rue de l'Ecole de Médecine, Paris, France.
authors.forename: Jerome
authors.initials: J
authors.lastname: Legoff
authors.forename: Clare
authors.initials: C
authors.lastname: Tanton
authors.forename: Maxime
authors.initials: M
authors.lastname: Lecerf
authors.forename: Gérard
authors.initials: G
authors.lastname: Grésenguet
authors.forename: Khonde
authors.initials: K
authors.lastname: Nzambi
authors.forename: Hicham
authors.initials: H
authors.lastname: Bouhlal
authors.forename: Helen
authors.initials: H
authors.lastname: Weiss
authors.forename: Laurent
authors.initials: L
authors.lastname: Belec
mesh_terms: D002584:Cervix Uteri / Q000378:metabolism / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005836:Genitalia, Female / Q000378:metabolism / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D018258:Herpesvirus 2, Human / Q000235:genetics*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis*; D012034:Refrigeration; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D014621:Vagina / Q000378:metabolism / Q000821:virology; D044364:Vaginal Douching
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral