No effect of anti-interleukin-5 therapy (mepolizumab) on the atopy patch test in atopic dermatitis patients.
BACKGROUND
The atopy patch test (APT) is an in vivo model to study the induction of eczema by inhalant allergens in atopic dermatitis (AD) patients. Mepolizumab is a monoclonal antibody to interleukin-5, which reduces peripheral blood eosinophils. Previously, we reported that mepolizumab treatment did not result in clinical improvement in AD. The current study investigates the effect of mepolizumab therapy on the APT in the same patients.


METHODS
Mepolizumab treatment was given at days 0 and 7 in a double-blind placebo-controlled design. The APT was applied at days -2, 0, 14 and 28. Clinical evaluation of each APT was conducted 48 h after application at days 0, 2, 16 and 30. Skin biopsies were taken at days 0, 2 and 16 for eosinophil counts.


RESULTS
The mepolizumab-treated group showed no significant reduction in macroscopic outcome of the APT. Tissue eosinophils were reduced in the mepolizumab-treated group at day 16 compared with placebo; however, this was not significant.


CONCLUSIONS
Mepolizumab therapy cannot prevent the eczematous reaction induced by the APT. Furthermore, the influx of tissue eosinophil numbers in the APT is not significantly inhibited after mepolizumab treatment compared with placebo, despite a significant reduction in peripheral blood eosinophils.
Oldhoff
J M
JM
Department of Dermatology and Allergology, UMC Utrecht, Utrecht, The Netherlands. j.m.oldhoff@umcutrecht.nl
Darsow
U
U
Werfel
T
T
Bihari
I C
IC
Katzer
K
K
Laifaoui
J
J
Plötz
S
S
Kapp
A
A
Knol
E F
EF
Bruijnzeel-Koomen
C A F M
CA
Ring
J
J
de Bruin-Weller
M S
MS
D000328:Adult; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D061067:Antibodies, Monoclonal, Humanized; D002452:Cell Count; D003876:Dermatitis, Atopic / Q000188:drug therapy* / Q000276:immunology* / Q000503:physiopathology; D004804:Eosinophils / Q000187:drug effects / Q000276:immunology; D005260:Female; D006801:Humans; D015848:Interleukin-5 / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010328:Patch Tests; D017211:Treatment Failure
D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D015848:Interleukin-5; C434107:mepolizumab