Doublecortin is necessary for the migration of adult subventricular zone cells from neurospheres.
Mutations in human doublecortin (DCX) and knockdown of Dcx in rodents cause radial migration defects in the embryonic cerebral cortex. However, the brain phenotype of Dcx knockout mice is largely normal suggesting that Dcx is not necessary for most migration events. Adult subventricular zone (SVZ) cells migrate tangentially in the rostral migratory stream to the olfactory bulbs. Dcx is expressed in the SVZ but it is unknown if it is necessary for migration. We show that Dcx RNAi reduced SVZ cell migration in vitro, both cell autonomously and non-cell autonomously. Overexpression of Dcx rescued migration after knockdown, but did not increase migration by itself. Thus, Dcx is necessary not only for embryonic radial migration but also migration of adult SVZ cells.
Ocbina
Polloneal J
PJ
Weill Cornell/Rockefeller/Sloan-Kettering, Tri-Institutional MD-PhD Program, 1300 York Avenue, Room D-115, New York, NY 10021-4896, USA.
Dizon
Maria L V
ML
Shin
Laura
L
Szele
Francis G
FG
D020413:3' Untranslated Regions; D000367:Age Factors; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000196:embryology / Q000254:growth & development*; D002465:Cell Movement / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D006367:HeLa Cells; D006801:Humans; D020547:Lateral Ventricles / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D018715:Microscopy, Video; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000502:physiology*; D009479:Neuropeptides / Q000235:genetics / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D010641:Phenotype; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D018874:Spheroids, Cellular / Q000166:cytology*
D020413:3' Untranslated Regions; C000714328:DCX protein, human; C000714847:Dcx protein, mouse; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D012333:RNA, Messenger