title: Detection of natural infections with Theileria annulata on calves at first theileriosis season: comparison of the Indirect Fluorescent Antibody Test (IFAT) and blood smears.
abstract: The Indirect Fluorescent Antibody Test (IFAT) remains so far the most commonly used test for sero-epidemiological investigations on tropical theileriosis (infection of cattle with Theileria annulata). The present studies evaluated the ability of both IFAT with schizont antigen (schizont IFAT) and blood smears to detect infected animals just after the theileriosis season. This evaluation was performed on a group of 89 calves of known infection status for T. annulata at first disease season, from farms with endemic stability for tropical theileriosis. An additional retrospective group of 84 cattle free of infection was also used for the estimation of the specificity of the schizont IFAT. The sensitivity and the specificity of schizont IFAT were 88.9% (64/72) and 97% (98/101), respectively. Blood smears showed a lower sensitivity of 63.9% (46/72). The agreement between the two detection techniques and the infection status of the animals, evaluated by the Kappa coefficient, was 0.85 and 0.64 for IFAT and blood smears, respectively.
authors.affiliation: Laboratory of Parasitology, Ecole Nationale de Médecine Vétérinaire, 2020 Sidi Thabet, Tunisia.
authors.forename: M A
authors.initials: MA
authors.lastname: Darghouth
authors.forename: L
authors.initials: L
authors.lastname: Sassi
authors.forename: M
authors.initials: M
authors.lastname: Gharbi
authors.forename: M C
authors.initials: MC
authors.lastname: Soudani
authors.forename: M
authors.initials: M
authors.lastname: Karoui
authors.forename: A
authors.initials: A
authors.lastname: Krichi
mesh_terms: D017677:Age Distribution; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis / Q000276:immunology; D001800:Blood Specimen Collection; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D019353:Endemic Diseases / Q000706:statistics & numerical data; D005455:Fluorescent Antibody Technique / Q000379:methods* / Q000592:standards; D010274:Parasitology / Q000379:methods* / Q000592:standards; D012189:Retrospective Studies; D012621:Seasons; D012680:Sensitivity and Specificity; D016796:Theileria annulata / Q000276:immunology*; D013801:Theileriasis / Q000097:blood* / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D014416:Tunisia / Q000453:epidemiology
chemical_list: D000953:Antigens, Protozoan