title: Phylogeny, physiology and distribution of 'Candidatus Microthrix calida', a new Microthrix species isolated from industrial activated sludge wastewater treatment plants.
abstract: Twelve strains of filamentous bacteria morphologically identified as 'Microthrix parvicella' were isolated from industrial activated sludge wastewater treatment plants. 16S rRNA gene sequences analysis showed that these strains were all closely related to 'Candidatus Microthrix parvicella'. Six of them, however, had a 16S rRNA gene similarity of only 95.7% and 96.7% to 'Candidatus Microthrix parvicella' suggesting the presence of a new species. The name 'Candidatus Microthrix calida' is proposed for this new microorganism. The physiological properties of these six isolates supported the description of a new taxon. The 'Candidatus Microthrix calida' strains produced thin filaments (0.3-0.7 microm diameter), they did not grow on the media supporting the growth of 'Candidatus Microthrix parvicella' and could be cultivated at higher temperature (up to 36.5 degrees C). Preliminary data on substrate uptake were obtained by microautoradiography on pure culture. Two new fluorescence in situ hybridization (FISH) probes, Mpa-T1-1260 specific for 'Candidatus Microthrix calida' and Mpa-all-1410 targeting both Microthrix species, were designed. The presence of Microthrix spp. was investigated in 114 activated sludge plants. 'Microthrix parvicella' morphotype was detected in 23% of the analysed samples and FISH analysis revealed that 'Candidatus Microthrix calida' was present in 5% of them. The remaining 'M. parvicella' filaments were positive with probe Mpa-all-1410 but could not all be identified as 'Candidatus Microthrix parvicella' suggesting the presence of more hitherto undescribed biodiversity within this morphotype.
authors.affiliation: CNR, Water Research Institute, Via Reno 1, 00198 Rome, Italy. levantesi@irsa.cnr.it
authors.forename: Caterina
authors.initials: C
authors.lastname: Levantesi
authors.forename: Simona
authors.initials: S
authors.lastname: Rossetti
authors.forename: Karin
authors.initials: K
authors.lastname: Thelen
authors.forename: Caroline
authors.initials: C
authors.lastname: Kragelund
authors.forename: Janneke
authors.initials: J
authors.lastname: Krooneman
authors.forename: Dick
authors.initials: D
authors.lastname: Eikelboom
authors.forename: Per Halkjaer
authors.initials: PH
authors.lastname: Nielsen
authors.forename: Valter
authors.initials: V
authors.lastname: Tandoi
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000502:physiology; D000818:Animals; D017404:In Situ Hybridization, Fluorescence; D008372:Manure / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000145:classification; D012722:Sewage / Q000382:microbiology*
chemical_list: D008372:Manure; D012336:RNA, Ribosomal, 16S; D012722:Sewage