Protective role of S-adenosyl-L-methionine against hydrochloric acid stress in Saccharomyces cerevisiae.
S-adenosyl-L-methionine (AdoMet, 1mM) protects the stationary phase cells of Saccharomyces cerevisiae against the killing effect of acid (10mM HCl, pH approximately 2). Both the acid and the acid plus AdoMet treatment for 2h increased the plasma membrane H(+)-ATPase activity; thereafter it decreased to the basal level. AdoMet partially recovered the intracellular pH (pH(in)) that dropped in presence of acid. AdoMet treatment facilitated acid induced phospholipid biosynthesis as well as membrane proliferation, which was reflected in the cellular lipid composition.
Malakar
Dipankar
D
Department of Biotechnology, Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700032, India.
Dey
Anindya
A
Ghosh
Anil K
AK
D019610:Cytoprotection / Q000187:drug effects*; D006851:Hydrochloric Acid / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000648:ultrastructure
D012436:S-Adenosylmethionine; D006851:Hydrochloric Acid