The effects of Gingko biloba extract on acetic acid-induced colitis in rats.
OBJECTIVE
Gingko biloba is an antioxidant substance which has antagonistic activity on platelet-activating factor. We aimed to investigate the antioxidant effect and the histopathologic changes caused by Gingko biloba on acetic acid-induced colitis.


METHODS
Totally 22 rats were divided into three groups. Group 1 (n=7) served as the control group. Group 2 (n=7) and Group 3 (n=8) were given 2 ml/day of 4% acetic acid by intracolonic instillation for three days. Gingko biloba (100 mg/kg) was then given only to Group 3 intraperitoneally for three days. Oxidative stress was assessed by determinate tissue and serum malondialdehyde (MDA) levels, and colonic damage was assessed by histologic examination.


RESULTS
Depth of necrosis, extent of necrosis, degree of inflammation, extent of inflammation, fibrosis and total histologic scores in Group 2 were significantly higher than in the control group (p<0.05). The same parameters were lower in Group 3 versus Group 2, but the difference was not significant. Tissue and serum MDA levels in Group 2 were significantly higher than Group 1 (p<0.01 and 0.05, respectively). Again, the same parameters in Group 3 were lower than in Group 2, but the difference was not significant statistically.


CONCLUSIONS
Gingko biloba did not significantly affect histopathological and oxidative stress parameters in experimental colitis.
Harputluoglu
Murat M
MM
Department of Gastroenterology, Medical Faculty, Inönü University, Malatya, Turkey. mharputluoglu@hotmail.com
Demirel
Ulvi
U
Yücel
Neslihan
N
Karadağ
Neşe
N
Temel
Ismail
I
Firat
Serpil
S
Ara
Cengiz
C
Aladağ
Murat
M
Karincaoğlu
Melih
M
Hilmioğlu
Fatih
F
D019342:Acetic Acid / Q000009:adverse effects*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015415:Biomarkers / Q000097:blood; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D005355:Fibrosis / Q000139:chemically induced / Q000517:prevention & control; D020441:Ginkgo biloba; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009336:Necrosis / Q000139:chemically induced / Q000517:prevention & control; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D015415:Biomarkers; D010936:Plant Extracts; D010972:Platelet Activating Factor; D008315:Malondialdehyde; D019342:Acetic Acid