title: [Effect of parathyroid hormone (PTH 1-34) on hemopoiesis in long-term cultures of human bone marrow].
abstract: OBJECTIVE
  To study effects of parathyroid hormone (PTH) used in therapy of osteoporosis on hemopoiesis in long-term culture of human bone marrow (LCBM) in terms of its potential influence on stem hemopoietic and stromal cells.
  
  
  METHODS
  For a long time LCBM was treated with PTH (1-34) and compared for cell production, concentration of late and early hemopoietic precursors. Maintenance of hemopoiesis and adhesion at early hemopoiesis precursors on the stromal sublayers treated with PTH (1-34) was used as a functional test. A relative level of expression of genes participating in regulating proliferation and self-support of stem hemopoietic cells was studied.
  
  
  RESULTS
  PTH (1-34) in the above concentrations did not affect hemopoiesis in LCBM. Stromal sublayer treated with PTH (1-34) for a long time supports cell precursors better, their adhesion to such sublayers enhances.
  
  
  CONCLUSIONS
  PTH (1-34) in pharmacological but small concentrations had no irreversible effects on hemopoiesis, i.e. contraindications for its use in the treatment of osteoporosis were not revealed.
authors.forename: D A
authors.initials: DA
authors.lastname: Svinareva
authors.forename: I N
authors.initials: IN
authors.lastname: Nifontova
authors.forename: K S
authors.initials: KS
authors.lastname: Momotiuk
authors.forename: V G
authors.initials: VG
authors.lastname: Savchenko
authors.forename: I L
authors.initials: IL
authors.lastname: Chertkov
authors.forename: N I
authors.initials: NI
authors.lastname: Drize
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D010281:Parathyroid Hormone; D010446:Peptide Fragments