Regulation of avilamycin biosynthesis in Streptomyces viridochromogenes: effects of glucose, ammonium ion, and inorganic phosphate.
Effects of glucose, ammonium ions and phosphate on avilamycin biosynthesis in Streptomyces viridochromogenes AS4.126 were investigated. Twenty grams per liter of glucose, 10 mmol/L ammonium ions, and 10 mmol/L phosphate in the basal medium stimulated avilamycin biosynthesis. When the concentrations of glucose, ammonium ions, and phosphate in the basal medium exceeded 20 g/L, 10 mmol/L, and 10 mmol/L, respectively, avilamycin biosynthesis greatly decreased. When 20 g/L glucose was added at 32 h, avilamycin yield decreased by 70.2%. Avilamycin biosynthesis hardly continued when 2-deoxy-glucose was added into the basal medium at 32 h. There was little influence on avilamycin biosynthesis with the addition of the 3-methyl-glucose (20 g/L) at 32 h. In the presence of excess (NH4)2SO4 (20 mmol/L), the activities of valine dehydrogenase and glucose-6-phosphate dehydrogenase were depressed 47.7 and 58.3%, respectively, of that of the control at 48 h. The activity of succinate dehydrogenase increased 49.5% compared to the control at 48 h. The intracellular adenosine triphosphate level and 6-phosphate glucose content of S. viridochromogenes were 128 and 129%, respectively, of that of the control at 48 h, with the addition of the 40 mmol/L of KH2PO4. As a result, high concentrations of glucose, ammonium ions, and inorganic phosphate all led to the absence of the precursors for avilamycin biosynthesis and affected antibiotic synthesis.
Zhu
Chuan-he
CH
Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, 30022, Tianjin, People's Republic of China.
Lu
Fu-ping
FP
He
Ya-nan
YN
Han
Zhen-lin
ZL
Du
Lian-xiang
LX
D019325:3-O-Methylglucose / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D003847:Deoxyglucose / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D009844:Oligosaccharides / Q000096:biosynthesis*; D010710:Phosphates / Q000494:pharmacology*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013302:Streptomyces / Q000378:metabolism*; D050802:Valine Dehydrogenase (NADP+) / Q000037:antagonists & inhibitors
D003470:Culture Media; D004791:Enzyme Inhibitors; D009844:Oligosaccharides; D010710:Phosphates; D000644:Quaternary Ammonium Compounds; D019325:3-O-Methylglucose; C006653:avilamycin; D003847:Deoxyglucose; D050802:Valine Dehydrogenase (NADP+); C085852:glucose-3-phosphatase; D010744:Phosphoric Monoester Hydrolases; D005947:Glucose