Upregulation of interferon-alpha and RANTES in the cervix of HIV-1-seronegative women with high-risk behavior.
OBJECTIVE
The expression of innate immune molecules associated with potential blocking activity of HIV-1 propagation was analyzed in the cervical tissue of a group of African HIV-1 IgG-negative commercial sex workers (CSWs) with an HIV-1-encountering risk behavior.


METHODS
Cervical biopsies from the superior portion of the ectocervix were assessed for innate immune molecules and evaluated in situ by computerized image analysis at the single-cell level.


RESULTS
A higher expression of interferon-alpha (IFNalpha) and RANTES was detected in CSWs and HIV-1-infected individuals as compared to low-risk HIV-1-uninfected controls (Neg Ctrls). Most (>90%) of RANTES-expressing cells were CD8 cells as determined by confocal microscopy. In contrast, the expression of leukemia inhibitory factor (LIF) and secretory leukocyte protease inhibitor (SLPI) was comparable between the groups. The expression of beta-defensin 2 was highest in HIV-1-infected individuals.


CONCLUSIONS
Induction of IFNalpha and RANTES expression in cervical mucosa may contribute to protection of sexual HIV-1 transmission in subjects with a higher risk behavior.
Hirbod
Taha
T
Center for Molecular Medicine, Infectious Diseases Unit, Department of Medicine, Karolinska Institute, Karolinska University Hospital, Solna, Sweden.
Nilsson
Jakob
J
Andersson
Sonia
S
Uberti-Foppa
Caterina
C
Ferrari
Davide
D
Manghi
Mara
M
Andersson
Jan
J
Lopalco
Lucia
L
Broliden
Kristina
K
D000293:Adolescent; D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000821:virology; D002584:Cervix Uteri / Q000166:cytology / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D018946:Chemokine CCL5 / Q000096:biosynthesis* / Q000276:immunology; D005260:Female; D018023:HIV Seronegativity; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis; D012309:Risk-Taking; D011477:Sex Work; D015854:Up-Regulation
D018946:Chemokine CCL5; D016898:Interferon-alpha