title: Cyclops: new modular software suite for cryo-EM.
abstract: Cyclops is a new computer program designed as a graphical front-end that allows easy control and interaction with tasks and programs for 3D reconstruction of biological complexes using cryo-electron microscopy. Cyclops' current plug-ins are designed for automated particle picking and include two new algorithms, automated carbon masking and quaternion based rotation space sampling, which are also presented here. Additional plug-ins are in the pipeline. Cyclops allows straightforward organization and visualization of all data and tasks and allows both interactive and batch-wise processing. Furthermore, it was designed for straightforward implementation in grid architectures. As a front-end to a collection of programs it provides a common interface to these programs, thus enhancing the usability of the suite and the productivity of the user.
authors.affiliation: Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands. plaisier@chem.leidenuniv.nl
authors.forename: J R
authors.initials: JR
authors.lastname: Plaisier
authors.forename: L
authors.initials: L
authors.lastname: Jiang
authors.forename: J P
authors.initials: JP
authors.lastname: Abrahams
mesh_terms: D000465:Algorithms; D018833:Chaperonins / Q000737:chemistry; D019295:Computational Biology; D020285:Cryoelectron Microscopy / Q000379:methods*; D029968:Escherichia coli Proteins / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000737:chemistry; D012269:Ribosomal Proteins / Q000737:chemistry; D012984:Software; D012985:Software Design
chemical_list: D029968:Escherichia coli Proteins; C028715:GroE protein, E coli; D006360:Heat-Shock Proteins; D012269:Ribosomal Proteins; D018833:Chaperonins