[Influence of chronic ethanol intake insulin sensitivity and IR, IRS-1, IRS-2 mRNA expression of liver in male rats].
OBJECTIVE
To investigate the effect of ethanol intake on the insulin sensitivity of hepatic tissue in male rats and explores its possible molecular mechanisms.


METHODS
Wister rats were administrated ethanol at a dose of 0 (control), 0.8 (low ethanol, L), 1.6 (medium ethanol, M) and 2.4 g/kg (high ethanol, H) daily, respectively. Each group had ten males. Nineteen weeks later, serum was collected for testing of fasting plasma glucose and insulin. Total liver RNA was extracted. The expression levels of IR, IRS-1, IRS-2 mRNA in hepatic tissue were detected by RT-PCR.


RESULTS
Compared with the control group, the glucose concentration significantly increased in H ethanol groups (P < 0.05), the insulin concentration significantly increased in L and M ethanol groups (P < 0.05), HOMA-IR significantly increased in every ethanol group (P < 0.05). IR mRNA expression remarkably decreased in every ethanol group. IRS-1, IRS-2 mRNA expression increased in L and M ethanol groups but decreased in H ethanol group.


CONCLUSIONS
The present study showed that ethanol abuse could induce to insulin resistance. The decreased of IR, IRS-1, IRS-2 mRNA expression of liver may be involved in the molecular mechanism of the effects of ethanol on insulin insensitivity.
Zhou
Shao-Liang
SL
Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Hao
Li-Ping
LP
Chen
Yi-Ying
YY
Qu
Wei
W
D000818:Animals; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics / Q000378:metabolism*; D007333:Insulin Resistance; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism*
D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; C526299:Irs2 protein, rat; D012333:RNA, Messenger; D000431:Ethanol; D011972:Receptor, Insulin