Structure and polymer form of poly-3-hydroxyalkanoates produced by Pseudomonas oleovorans grown with mixture of sodium octanoate/undecylenic acid and sodium octanoate/5-phenylvaleric acid.
PHAs (poly-3-hydroxyalkanoates) obtained by Pseudomonas oleovorans grown with mixed carbon sources were investigated. Mixed carbon sources were sodium octanoate/undecylenic acid and sodium octanoate/5-phenylvaleric acid. Effect of carbon source in pre-culture on PHAs structure was investigated. Main fermentation was conducted with mixture of sodium octanoate/undecylenic acid, and PHA contained both saturated and unsaturated units. When more undecylenic acid was used in the medium, the ratio of unsaturated unit increased and the T(g) of the products also changed. The PHA grown with mixture of sodium octanoate and undecylenic acid was a random copolymer, which was determined by DSC analysis. Using mixed carbon sources of sodium octanoate and 5-phenylvaleric acid, highest dry cell weight and PHA concentration were obtained when 0.02g or 0.04g of 5-phenylvaleric acid were added in 50mL medium. Cultured with sodium octanoate and 5-phenylvaleric acid, PHA containing HO (3-hydroxyoctanoate) unit and HPV (3-hydroxy-5-phenylvalerate) unit was produced. T(g) of the products fell between those of pure PHO and pure PHPV. By means of DSC analysis and fractionation method, the PHA obtained was regarded as a random copolymer.
Ho
I-Ching
IC
Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan, ROC.
Yang
Sheng-Pin
SP
Chiu
Wen-Yen
WY
Huang
Shih-Yow
SY
D001704:Biopolymers / Q000096:biosynthesis* / Q000737:chemistry*; D002210:Caprylates / Q000032:analysis; D018929:Cell Culture Techniques; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D010421:Pentanoic Acids / Q000032:analysis; D044204:Pseudomonas oleovorans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D014476:Undecylenic Acids / Q000032:analysis
D001704:Biopolymers; D002210:Caprylates; D003470:Culture Media; D010421:Pentanoic Acids; D014476:Undecylenic Acids; C031492:octanoic acid; C400347:5-phenylvaleric acid