An experimental model of peripheral neuropathy induced in rats by Karwinskia humboldtiana (buckthorn) fruit.
Intoxication by Karwinskia humboldtiana (buckthorn) fruit presents a neurological picture similar to that of Guillain-Barré syndrome. In this report, we describe an experimental animal model of peripheral neuropathy induced by buckthorn fruit. Four groups of Wistar rats received one oral dose of 1.5 g/kg followed by oral doses of 0.5 g/kg at days 3, 7, 10, and 14 of dried and ground buckthorn fruit in aqueous suspension. Rats were sacrificed at 24, 48, 58, and 112 days after initial dose. Treated animals developed progressive paralysis through 58 days, then completely recovered by 112 days. Sciatic nerves showed segmental demyelination and cellular infiltrates until 58 days after exposure and then remyelinating changes at 112 days. This experimental model for peripheral neuropathy is reproducible and easy to handle. Its manipulation is relatively innocuous and allows us to study reversible peripheral nerve damage. This model can be developed in other animal species and may be useful to test new therapies for peripheral neuropathy.
Salazar-Leal
Martha E
ME
Departamento de Farmacologia y Toxicologia, Hospital Universitario y Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Monterrey, Mexico.
Flores
M S
MS
Sepulveda-Saavedra
Julio
J
Romero-Diaz
Viktor J
VJ
Becerra-Verdin
Eduardo M
EM
Tamez-Rodriguez
Victor A
VA
Martinez
Hector R
HR
Piñeyro-Lopez
Alfredo
A
Bermudez
M V
MV
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D005638:Fruit / Q000633:toxicity*; D031956:Karwinskia / Q000633:toxicity*; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000379:methods; D010523:Peripheral Nervous System Diseases / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D013997:Time Factors