[Detecting poliovirus in wastewater from Armenia, Colombia].
OBJECTIVE
Implementing environmental surveillance for poliovirus circulating from wastewater.


METHODS
Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. Polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and Sabin vaccine-specific primers.


RESULTS
It was confirmed that the viral detection method is highly efficient. Poliovirus was detected in 5 of 6 (83%) samples collected. Serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. All of them were of the vaccine type, which provided evidence regarding the non-circulation of wild poliovirus in the country.


CONCLUSIONS
Wastewater surveillance can be a sensitive tool for detecting poliovirus circulation.
González
Maria M
MM
Centro de Investigaciones Biomédicas, Facultad Ciencias de la Salud, Universidad del Quindío. mechotas2004@yahoo.com
Sarmiento
Luis
L
Castaño
Jhon Carlos
JC
Giraldo
Alejandra M
AM
Salazar
Alexander
A
Muñoz
Nini J
NJ
D003105:Colombia; D004783:Environmental Microbiology; D017955:Poliovirus / Q000145:classification / Q000302:isolation & purification*; D011055:Poliovirus Vaccine, Oral; D011092:Polyethylene Glycols; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012722:Sewage / Q000821:virology*; D012965:Sodium Chloride; D014773:Virology / Q000379:methods
D011055:Poliovirus Vaccine, Oral; D012367:RNA, Viral; D012722:Sewage; D011092:Polyethylene Glycols; D012965:Sodium Chloride