Salivary immunoglobulin A directed to oral microbial GroEL in patients with periodontitis and their potential protective role.
The aim of this study was to identify salivary immunoglobulin A (IgA) directed to oral microbial GroEL in patients with periodontitis and to demonstrate their potential protective role through a reduction of inflammatory cytokine production induced by microbial GroEL. Using five different proteins belonging to the heat-shock protein 60 family, Western immunoblot analysis of salivary IgA from 63 subjects revealed immunoreactivities with Campylobacter rectus GroEL and Porphyromonas gingivalis GroEL in subjects with periodontitis (P < 0.05) compared to control subjects. Using the BIACORE 1000 to measure the salivary IgA titers directed towards C. rectus GroEL, high resonance unit (RU) values were observed in the saliva samples from patients with periodontitis (P < 0.01). Furthermore, the number of teeth with deep pocket depth (>or=5 mm) showed a high correlation coefficient with the RU value (r = 0.50, P < 0.01). C. rectus GroEL possessed the ability to stimulate the production of interleukin-6 by gingival fibroblasts. Interestingly, salivary IgA antibody directed to C. rectus GroEL caused a partial inhibition of interleukin-6 production. This study showed a relationship between high levels of salivary IgA directed to GroEL and periodontal disease severity. Although additional investigations are required, salivary IgA to GroEL may have a protective role by reducing the inflammatory response induced by GroEL derived from periodontopathogenic bacteria.
Fukui
M
M
Clinic of Preventive Dentistry, General Dentistry, The Tokushima University Hospital, Tokushima, Japan.
Hinode
D
D
Yokoyama
M
M
Tanabe
S
S
Yoshioka
M
M
D000328:Adult; D000937:Antigen-Antibody Reactions; D000942:Antigens, Bacterial / Q000276:immunology; D015153:Blotting, Western; D044883:Campylobacter rectus / Q000276:immunology; D016022:Case-Control Studies; D018834:Chaperonin 60 / Q000276:immunology*; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D015850:Interleukin-6 / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology* / Q000382:microbiology*; D012463:Saliva / Q000276:immunology*; D012471:Salivary Proteins and Peptides / Q000276:immunology; D018709:Statistics, Nonparametric
D000942:Antigens, Bacterial; D018834:Chaperonin 60; D007071:Immunoglobulin A, Secretory; D015850:Interleukin-6; D012471:Salivary Proteins and Peptides