title: Affinity separation of polyribonucleotide-binding human blood proteins.
abstract: Using affinity columns with immobilized poly(A), poly(G), poly(U), poly(C), and poly(A).poly(U) and poly(G) x poly(C) duplexes several polyribonucleotide-binding blood plasma proteins have been captured. Albumin and keratins K1 and K2e have been detected to bind polypurine tracts. The in vitro glycated albumin binds poly(A) and poly(G) more efficiently than the unmodified protein. The major polypyrimidine-binding blood plasma protein (28 kDa) can catalyze the hydrolysis of poly(U).
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 8 Lavrentyev Ave., 630090 Novosibirsk, Russia.
authors.forename: Yuliya V
authors.initials: YV
authors.lastname: Gerasimova
authors.forename: Irina V
authors.initials: IV
authors.lastname: Alekseyeva
authors.forename: Tatyana G
authors.initials: TG
authors.lastname: Bogdanova
authors.forename: Irina A
authors.initials: IA
authors.lastname: Erchenko
authors.forename: Natalya V
authors.initials: NV
authors.lastname: Kudryashova
authors.forename: Boris P
authors.initials: BP
authors.lastname: Chelobanov
authors.forename: Pavel P
authors.initials: PP
authors.lastname: Laktionov
authors.forename: Pavel V
authors.initials: PV
authors.lastname: Alekseyev
authors.forename: Tatyana S
authors.initials: TS
authors.lastname: Godovikova
mesh_terms: D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D002846:Chromatography, Affinity; D006801:Humans; D011131:Polyribonucleotides / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D011131:Polyribonucleotides; D016601:RNA-Binding Proteins