The structures of the cell wall teichoic acids from the thermophilic microorganism Geobacillus thermoleovorans strain Fango.
The structures of two teichoic acid fractions (TA1 and TA2) isolated from the thermophilic gram-positive bacterium Geobacillus thermoleovorans strain Fango were investigated by means of chemical and NMR spectroscopic methods. The most abundant species (TA1) exhibited a rather regular structure comprising two different repeating units of 1,3-glycerol phosphate nonstoichiometrically substituted by terminal-alpha-D-Gal p (t-alpha-D-Gal p). The second molecular species (TA2) presented a higher structural variability and t-alpha-D-Glc p and the disaccharides t-alpha-D-Glc pNAc-(1-->2)-alpha-D-Glc p and t-alpha-D-Glc pNAc-(1-->3)-alpha-D-Glc p were also present as minor substituents at O-2 of the glycerol phosphate residues. Minor substitution by alanine could also be detected.
Leone
Serena
S
Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico II, Via Cintia, 4 I-80126 Napoli, Italy.
Molinaro
Antonio
A
Romano
Ida
I
Nicolaus
Barbara
B
Lanzetta
Rosa
R
Parrilli
Michelangelo
M
Holst
Otto
O
D001406:Bacillaceae / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013682:Teichoic Acids / Q000737:chemistry* / Q000302:isolation & purification
D013682:Teichoic Acids