title: CD1 expression and CD1-restricted T cell activity in normal and tumour-bearing human liver.
abstract: CD1d-restricted natural killer T (NKT) cells expressing invariant Valpha14Jalpha18 T cell receptor alpha-chains are abundant in murine liver and are implicated in the control of malignancy, infection and autoimmunity. Invariant NKT cells have potent anti-metastatic effects in mice and phase I clinical trials involving their homologues in humans are ongoing. However, invariant NKT cells are less abundant in human liver ( approximately 0.5% of hepatic T cells) than in murine liver (up to 50%) and it is not known if other hepatic T cells are CD1-restricted. We have examined expression of CD1a, CD1b, CD1c and CD1d mRNA and protein in human liver and evaluated the reactivity of mononuclear cells (MNC) from histologically normal and tumour-bearing human liver specimens against these CD1 isoforms. Messenger RNA for all CD1 isotypes was detectable in all liver samples. CD1c and CD1d were expressed at the protein level by hepatic MNC. CD1d, only, was detectable at the cell surface, but CD1c and CD1d were found at an intracellular location in significant numbers of liver MNC. CD1b was not expressed by MNC from healthy livers but was detectable within MNC in all tumour samples tested. Hepatic T cells exhibited reactivity against C1R cells expressing transfected CD1c and CD1d, but neither CD1a nor CD1b. These cells secreted interferon-gamma (IFN-gamma) but not interleukin-4 (IL-4) upon stimulation. In contrast, similar numbers of peripheral T cells released 13- and 16-fold less IFN-gamma in response to CD1c and CD1d, respectively. CD1c and CD1d expression and T cell reactivity were not altered in tumour-bearing liver specimens compared to histologically normal livers. These data suggest that, in addition to invariant CD1d-restricted NKT cells, autoreactive T cells that recognise CD1c and CD1d and release inflammatory cytokines are abundant in human liver.
authors.affiliation: Education and Research Centre, St. Vincent's University Hospital, Elm Park, Dublin 4, Ireland.
authors.forename: Tony
authors.initials: T
authors.lastname: Kenna
authors.forename: Margaret
authors.initials: M
authors.lastname: O'Brien
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Hogan
authors.forename: Mark A
authors.initials: MA
authors.lastname: Exley
authors.forename: Steven A
authors.initials: SA
authors.lastname: Porcelli
authors.forename: John E
authors.initials: JE
authors.lastname: Hegarty
authors.forename: Cliona
authors.initials: C
authors.lastname: O'Farrelly
authors.forename: Derek G
authors.initials: DG
authors.lastname: Doherty
mesh_terms: D018949:Antigens, CD1 / Q000378:metabolism*; D001324:Autoantigens / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D008099:Liver / Q000276:immunology* / Q000378:metabolism; D008113:Liver Neoplasms / Q000276:immunology* / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism
chemical_list: D018949:Antigens, CD1; D001324:Autoantigens; D020033:Protein Isoforms; D012333:RNA, Messenger