Genome-scale loss-of-function screening with a lentiviral RNAi library.
The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens.
Root
David E
DE
Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA. droot@broad.mit.edu
Hacohen
Nir
N
Hahn
William C
WC
Lander
Eric S
ES
Sabatini
David M
DM
D000818:Animals; D005786:Gene Expression Regulation / Q000235:genetics*; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D051379:Mice; D034741:RNA, Small Interfering / Q000235:genetics*
D034741:RNA, Small Interfering