[Molecular taxonomy techniques used for yeast identification].
Due to the major impact of yeasts in human life based on the existence of pathogen yeast species and of species with biotechnological abilities, in the last few years new molecular techniques are performed for an accurate identification of natural isolates. Our study is aimed to review some of these techniques such as electrokariotyping by PFGE (Pulsed Field Gel Electrophoresis), estimation of the molar percentage of guanine and cytosine, the applications of PCR reaction in yeast identification using RAPD (Random amplified polymorphic DNA), UP-PCR (Universally Primed Polymerase Chain Reaction), MLST (Multilocus sequence typing) techniques, mtDNA and rDNA homology studies. Such molecular techniques complete the phenotypical characterization based on classical taxonomical tests allowing thus the polyphasic identification of the microorganisms.
Ghindea
Raluca
R
Universitatea Bucureşti, Facultatea de Biologie, Centrul de Cercetare, Formare şi Consultanţă in Microbiologie, Genetică şi Biotehnologie.
Csutak
Ortansa
O
Stoica
Ileana
I
Ionescu
Robertina
R
Soare
Simona
S
Pelinescu
Diana
D
Nohit
Ana-Maria
AM
Creangă
Oana
O
Vassu
Tatiana
T
D003596:Cytosine; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D016521:Electrophoresis, Gel, Pulsed-Field; D006147:Guanine; D006801:Humans; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D006147:Guanine; D003596:Cytosine