Differential biohydrogenation and isomerization of [U-(13)C]oleic and [1-(13)C]oleic acids by mixed ruminal microbes.
The additional mass associated with 13C in metabolic tracers may interfere with their metabolism. The comparative isomerization and biohydrogenation of oleic, [1-(13)C]oleic, and [U-13C]oleic acids by mixed ruminal microbes was used to evaluate this effect. The percent of stearic, cis-14 and -15, and trans-9 to -16 18:1 originating from oleic acid was decreased for [U-(13)C]oleic acid compared with [1-(13)C]oleic acid. Conversely, microbial utilization of [U-(13)C]oleic acid resulted in more of the 13C label in cis-9 18:1 compared with [1-(13)C]oleic acid (53.7 vs. 40.1%). The isomerization and biohydrogenation of oleic acid by ruminal microbes is affected by the mass of the labeled tracer.
Mosley
Erin E
EE
Department of Animal and Veterinary Science, University of Idaho, Moscow, Idaho 83844-2330, USA.
Nudda
Anna
A
Corato
Adolfo
A
Rossi
Erica
E
Jenkins
Tom
T
McGuire
Mark A
MA
D000704:Analysis of Variance; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000737:chemistry; D002417:Cattle; D006865:Hydrogenation; D007536:Isomerism; D009829:Oleic Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D012417:Rumen / Q000382:microbiology*; D013229:Stearic Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors; D044242:Trans Fatty Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
D002250:Carbon Radioisotopes; D009829:Oleic Acids; D013229:Stearic Acids; D044242:Trans Fatty Acids