Studies on biodegradation of nicotine by Arthrobacter sp. strain HF-2.
A nicotine-degrading bacterium, strain HF-2, was isolated from tobacco waste-contaminated soil and identified as a member of Arthrobacter sp. based on morphology, physiological tests, 16S rDNA sequence and phylogenetic characteristics. At thermal denaturation test indicated that the G + C mol% of strain HF-1 was 63.5. The relationship between the growth of the isolate and the nicotine degradation suggested that strain HF-2 could utilize nicotine as sole sources of carbon, nitrogen and energy. Blue pigment was observed during the nicotine degradation by strain HF-2. The isolate grew well at 20 to 33 degrees C, initial pH 6.5 to 8.0 and 0.5 to 2.0 g L-1 of nicotine concentration in the nicotine inorganic salt media. The maximum growth and nicotine degradation occurred at 30 degrees C, initial pH 7.0 and 0.7 g.L-1 of nicotine concentration in media under natural incubation condition. Strain HF-2 could degrade 100% of nicotine under the optimized incubation conditions for 43 h. The concentrations of nicotine were monitored by high performance liquid chromatography. This study demonstrates Arthrobacter sp. strain HF-2 had a great ability to degrade nicotine, and it may be available for the application to the bioremediation of environments contaminated by tobacco waste.
Ruan
Aidong
A
State Key Laboratory of Hydrology, Water Resource and Hydraulic Engineering Hohai University, Nanjing, PR China. aidong_ruan@hhu.edu.cn
Min
Hang
H
Zhu
Wei
W
D001173:Arthrobacter / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009538:Nicotine / Q000378:metabolism*; D010802:Phylogeny; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013696:Temperature
D004269:DNA, Bacterial; D012989:Soil Pollutants; D009538:Nicotine