title: [Effect of cytarabine on expression of cell adhesion molecules and on endothelium-leukocyte interaction in vitro].
abstract: OBJECTIVE
  To characterize cytarabine effects on human endothelium expression of cell adhesion molecules (CAM) of various classes and interaction of cultured endothelial cells (EC) with blood leukocytes.
  
  
  METHODS
  Primary cultures of EC of human umbilical vein were obtained by EC culturing for 24-72 h with cytarabine (10 mcg/ml). They were investigated with phase-contrast microscopy, immunohistochemistry and flow cytometry.
  
  
  RESULTS
  Cytarabine effects manifested with EC-induced expression of cell surface P- and E-selectines and VCAM-1; ICAM-1 expression rapidly enhanced, leukocyte adhesion to endothelium also activates.
  
  
  CONCLUSIONS
  Cytarabine, as a pathogenetic factor, induces proinflammatory alterations of the endothelium responsible for development of vascular complications in patients on cytostatic therapy.
authors.forename: A M
authors.initials: AM
authors.lastname: Chervontseva
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Romanov
authors.forename: V G
authors.initials: VG
authors.lastname: Savchenko
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000009:adverse effects*; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002478:Cells, Cultured; D003561:Cytarabine / Q000009:adverse effects*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000166:cytology*
chemical_list: D000964:Antimetabolites, Antineoplastic; D015815:Cell Adhesion Molecules; D003561:Cytarabine