title: A gradient HPLC method for the quality control of chlorogenic acid, linarin and luteolin in Flos Chrysanthemi Indici suppository.
abstract: The Flos Chrysanthemi Indici suppository, a famous traditional Chinese medicine, is marketed for the treatment of prostatitis and chronic pelvic inflammation. A simple, relatively rapid and accurate high-performance liquid chromatographic (HPLC) method was applied to the simultaneous determination of chlorogenic acid, linarin and luteolin in Flos Chrysanthemi Indici suppository. The good separation was achieved on a Hypersil ODS column by a gradient elution using the mixture of acetonitrile and aqueous 1.0% acetic acid as mobile phase within 23 min. The method was validated for three active phenolic compounds with the relative standard deviations (R.S.D.) of intra- and inter-day precision below 3.0%, and the detection limits (S/N=3) of chlorogenic acid, linarin and luteolin were 0.32, 0.08 and 0.05 microg ml(-1), respectively. This assay was successfully applied to the determination of three active phenolic compounds in Flos Chrysanthemi Indici suppository samples with the quantitative recoveries in the range of 94.7-101.8%. The results indicate that the developed HPLC method can be readily utilized as a quality control method for Flos Chrysanthemi Indici suppository and its related traditional Chinese medicinal preparations.
authors.affiliation: School of Pharmacy, Anhui Medical University, Hefei, Anhui 230032, PR China. qlzhang@ahmu.edu.cn
authors.forename: Qunlin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Zhongtang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Wenming
authors.initials: W
authors.lastname: Cheng
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031202:Chrysanthemum; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D006027:Glycosides / Q000032:analysis*; D047311:Luteolin / Q000032:analysis*; D015394:Molecular Structure; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013488:Suppositories; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000097:Acetonitriles; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D012997:Solvents; D013488:Suppositories; D002726:Chlorogenic Acid; C008282:linarin; D047311:Luteolin; D019342:Acetic Acid; C032159:acetonitrile