title: Antihyperglycemic effect of polyphenols from Acerola (Malpighia emarginata DC.) fruit.
abstract: A crude acerola polyphenol fraction (C-AP) was prepared by subjecting an acerola extract to a C18 cartridge column, and eluting the adsorbed fraction with ethanol containing 10% of acetic acid. C-AP appeared in a previous study to have an inhibitory effect on alpha-glucosidase and particularly on maltase activities. To elucidate the antihyperglycemic effect of C-AP further, we examined the regulation by C-AP of glucose uptake in Caco-2 cell; this resulted in the inhibition of glucose uptake. We next conducted single administration tests of glucose and maltose to ICR mice to investigate whether C-AP really controlled the intestinal glucose absorption in an animal body. The results showed that C-AP significantly suppressed the plasma glucose level after administering both glucose and maltose, suggesting that C-AP had a preventive effect on hyperglycemia in the postprandial state. The mechanism for this effect is considered to have been both suppression of the intestinal glucose transport and the inhibition of alpha-glucosidase. Despite such a preventive effect, the therapeutic effect of C-AP on hyperglycemia appeared to be low from the experiment with KKAy mice.
authors.affiliation: Research and Development Division, Nichirei Foods Inc., Chiba, Japan. hanamurat@nichirei.co.jp
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hanamura
authors.forename: Chisato
authors.initials: C
authors.lastname: Mayama
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Aoki
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hirayama
authors.forename: Makoto
authors.initials: M
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D018938:Caco-2 Cells; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000188:drug therapy*; D004195:Disease Models, Animal; D005419:Flavonoids / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology; D005951:Glucose Tolerance Test; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D029581:Malpighiaceae / Q000737:chemistry*; D008320:Maltose / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D008820:Mice, Obese; D015394:Molecular Structure; D010636:Phenols / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols; D013237:Stereoisomerism; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D005419:Flavonoids; D007004:Hypoglycemic Agents; D010636:Phenols; D059808:Polyphenols; D008320:Maltose; D005947:Glucose