Array analysis applied to malformed hearts: Molecular dissection of tetralogy of Fallot.
Microarray technology as a method for large-scale gene expression analysis has entered into widespread use in the field of cardiovascular research. This chapter summarizes the application of arrays to study gene expression profiles of congenital heart diseases, in particular the molecular portrait of tetralogy of Fallot. The sections of this chapter correspond to the several distinct steps of microarray experiments. A general introduction to the method, and information on the selection of arrays and preparation of labeled complementary DNA samples are given. A specific focus of the chapter is the experimental design and data analysis.
Sperling
Silke
S
Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany.
D018076:DNA, Complementary / Q000096:biosynthesis; D020869:Gene Expression Profiling; D006801:Humans; D017403:In Situ Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000302:isolation & purification; D013771:Tetralogy of Fallot / Q000235:genetics*
D018076:DNA, Complementary; D012313:RNA