title: Biosurfactant production by Rhodococcus erythropolis grown on glycerol as sole carbon source.
abstract: The production of biosurfactant by Rhodococcus erythropolis during the growth on glycerol was investigated. The process was carried out at 28 degrees C in a 1.5-L bioreactor using glycerol as carbon source. The bioprocess was monitored through measurements of biosurfactant concentration and glycerol consumption. After 51 h of cultivation, 1.7 g/L of biosurfactant, surface, and interfacial tensions values (with n-hexadecane) of 43 and 15 mN/m, respectively, 67% of Emulsifying Index (E24), and 94% of oil removal were obtained. The use of glycerol rather than what happens with hydrophobic carbon source allowed the release of the biosurfactant, originally associated to the cell wall.
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Rio de Janeiro, RJ, CEP 21949-900, Brasil.
authors.forename: Elisa M P
authors.initials: EM
authors.lastname: Ciapina
authors.forename: Walber C
authors.initials: WC
authors.lastname: Melo
authors.forename: Lidia M M
authors.initials: LM
authors.lastname: Santa Anna
authors.forename: Alexandre S
authors.initials: AS
authors.lastname: Santos
authors.forename: Denise M G
authors.initials: DM
authors.lastname: Freire
authors.forename: Nei
authors.initials: N
authors.lastname: Pereira Júnior
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D005990:Glycerol / Q000378:metabolism*; D012240:Rhodococcus / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D013501:Surface-Active Agents / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D012722:Sewage; D013501:Surface-Active Agents; D002244:Carbon; D005990:Glycerol