Identification and molecular detection of a deletion mutation responsible for a truncated cadherin of Helicoverpa armigera.
Cadherins are a class of receptor proteins for Bacillus thuringiensis Cry1A toxins. Disruption of a cadherin gene (Ha_BtR) is associated with Cry1Ac resistance in the cotton bollworm Helicoverpa armigera [Xu, X., Yu, L., Wu, Y., 2005. Disruption of a cadherin gene associated with resistance to Cry1Ac delta-endotoxin of B. thuringiensis in H. armigera. Appl. Environ. Microbiol. 71, 948-954]. Determination of the genomic DNA sequences of Ha_BtR gene showed that the wild type Ha_BtR coding sequence is comprised of 34 exons. A deletion between Exon 8 and Exon 25 was found to be responsible for a truncated cadherin in the Cry1Ac-resistant GYBT strain of H. armigera. The mutant allele of Ha_BtR (r1) has two possible transcription variants, both of which produce the same truncated protein. A DNA-based detection method specific to the r1 allele was developed. This study will facilitate the monitoring of cadherin mutant frequency in field populations of H. armigera.
Yang
Yajun
Y
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
Chen
Haiyan
H
Wu
Shuwen
S
Yang
Yihua
Y
Xu
Xinjun
X
Wu
Yidong
Y
D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000302:isolation & purification*; D005091:Exons; D017353:Gene Deletion; D007438:Introns; D007915:Lepidoptera / Q000235:genetics*; D009154:Mutation
D015820:Cadherins; C099972:H-cadherin