title: Characterization of blood components prepared from whole-blood donations after a 24-hour hold with the platelet-rich plasma method.
abstract: BACKGROUND
  The preparation of platelet (PLT) concentrates (PCs) from PLT-rich plasma (PRP) requires that whole blood (WB) be processed within 8 hours of collection. Increasing WB storage time to 24 hours would be logistically attractive. This study compares the in vitro quality of blood components prepared from WB stored for 8 and 24 hours at room temperature before processing with the PRP method.
  
  
  METHODS
  WB units were collected from ABO-matched blood donors. To reduce individual variations, paired donations were drawn in parallel, pooled, and split back in the collection bag. One unit was held for 6 to 8 hours and the other for 22 to 24 hours at 20 to 24 degrees C. Prestorage leukoreduced components were prepared with the PRP as intermediate product and analyzed during storage.
  
  
  RESULTS
  RBC units prepared after an 8- or 24-hour hold were comparable in terms of hemolysis, sodium, pH, and ATP levels. RBC 2,3- diphosphoglycerate (2,3-DPG) was significantly lower in RBCs prepared from 24-hour hold donations immediately after processing but not after 20 days of storage. Residual white blood cells were approximately fivefold higher (p < 0.05) in 24-hour RBC units. For PCs, measurements for glucose, ATP, lactate, pH, extent of shape change, hypotonic shock response, and CD62p activation were similar. No differences were observed in the von Willebrand factor, factor (F)V, FVIII, and fibrinogen content of fresh-frozen plasma.
  
  
  CONCLUSIONS
  The decrease in FVIII and RBC 2,3-DPG can be acceptable as a compromise to improve blood component logistics, but leukoreduction efficiency must be improved before considering the adoption of an overnight storage of WB before PRP processing.
authors.affiliation: Department of Operational Research, R&D, Héma-Québec, Sainte-Foy, Québec, Canada. louis.thibault@hema-quebec.qc.ca
authors.forename: Louis
authors.initials: L
authors.lastname: Thibault
authors.forename: Annie
authors.initials: A
authors.lastname: Beauséjour
authors.forename: Marie Joëlle
authors.initials: MJ
authors.lastname: de Grandmont
authors.forename: Réal
authors.initials: R
authors.lastname: Lemieux
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Leblanc
mesh_terms: D000017:ABO Blood-Group System; D000255:Adenosine Triphosphate / Q000097:blood; D001779:Blood Coagulation Factors / Q000032:analysis; D001782:Blood Donors; D001786:Blood Glucose / Q000032:analysis; D001792:Blood Platelets / Q000378:metabolism; D001800:Blood Specimen Collection / Q000379:methods; D019040:E-Selectin / Q000097:blood; D005260:Female; D006461:Hemolysis; D006801:Humans; D047589:Leukocyte Reduction Procedures / Q000379:methods; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D010949:Plasma; D013997:Time Factors
chemical_list: D000017:ABO Blood-Group System; D001779:Blood Coagulation Factors; D001786:Blood Glucose; D019040:E-Selectin; D000255:Adenosine Triphosphate