title: Identification of cell adhesion molecules in the human follicle-associated epithelium that improve nanoparticle uptake into the Peyer's patches.
abstract: The aim of this study was to identify cell adhesion molecules that could serve as targets of the human follicle-associated epithelium (FAE) overlying Peyer's patches and to assess nanoparticle uptake levels across this epithelium. We first studied the expression of the mouse M-cell marker beta(1)-integrin and used a model of human FAE derived from intestinal epithelial Caco-2 cells and Raji B-cells to identify additional potential targets by cDNA array. The protein expression of potential targets in the model FAE and in human ileal FAE tissues was quantified by immunofluorescence. Integrin targeting was studied by investigating the transport of Arg-Gly-Asp (RGD)-coated (integrin-binding), Arg-Gly-Glu (RGE)-coated (nonintegrin-binding), and uncoated nanoparticles across ileal specimens mounted in Ussing chambers. Both beta(1)-integrin and the cell adhesion molecule CD9 were more abundantly expressed in the model and human FAE compared with the Caco-2 control cells or villus epithelium (VE). Uncoated nanoparticles were not taken up across either FAE or VE. General integrin targeting with RGD improved the nanoparticle transport dramatically across the FAE and to a lower extent across the VE. Compared with RGE, RGD improved transport 4-fold across the FAE. There was no difference in the transport of RGD- and RGE-coated nanoparticles across the VE. In conclusion, beta(1)-integrin and CD9 were identified as targets in human FAE. The difference in RGD- and RGE-mediated transport across the FAE, but not the VE, suggests that a specific integrin interaction was the dominating mechanism for improved nanoparticle uptake across the FAE., whereas charge interaction contributed substantially to the improved VE uptake.
authors.affiliation: Department of Pharmacy, Box 580, BMC, SE-751 23 Uppsala, Sweden.
authors.forename: Elisabet
authors.initials: E
authors.lastname: Gullberg
authors.forename: Asa V
authors.initials: AV
authors.lastname: Keita
authors.forename: Sa'ad Y
authors.initials: SY
authors.lastname: Salim
authors.forename: Margaretha
authors.initials: M
authors.lastname: Andersson
authors.forename: Karin D
authors.initials: KD
authors.lastname: Caldwell
authors.forename: Johan D
authors.initials: JD
authors.lastname: Söderholm
authors.forename: Per
authors.initials: P
authors.lastname: Artursson
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D001692:Biological Transport; D018938:Caco-2 Cells; D004848:Epithelium / Q000378:metabolism*; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D053758:Nanoparticles; D020411:Oligonucleotide Array Sequence Analysis; D009842:Oligopeptides / Q000378:metabolism; D010581:Peyer's Patches / Q000378:metabolism*; D060245:Tetraspanin 29
chemical_list: D015703:Antigens, CD; C554869:CD9 protein, human; C554870:Cd9 protein, mouse; D019012:Integrin beta1; D008562:Membrane Glycoproteins; D009842:Oligopeptides; D060245:Tetraspanin 29; C112658:arginyl-glycyl-glutamic acid; C047981:arginyl-glycyl-aspartic acid