title: High-resolution fine mapping of ps-2, a mutated gene conferring functional male sterility in tomato due to non-dehiscent anthers.
abstract: Functional male sterility is an important trait for the production of hybrid seeds. Among the genes coding for functional male sterility in tomato is the positional sterility gene ps-2. ps-2 is monogenic recessive, confers non-dehiscent anthers and is the most suitable for practical uses. In order to have tools for molecular-assisted selection (MAS) we fine mapped the ps-2 locus. This was done in an F(2) segregating population derived from the interspecific cross between a functionally male sterile line (ps-2/ps-2; Solanum lycopersicum) and a functionally male fertile line (S. pimpinellifolium). Here we report the procedure that has led to the high-resolution fine mapping of the ps-2 locus in a 1.65 cM interval delimited by markers T0958 and T0635 on the short arm of Chromosome 4. The presence of many COS markers in the local high-resolution map allowed us to study the synteny between tomato and Arabidopsis at the ps-2 locus region. No obvious candidate gene for ps-2 was identified among the known functional male sterility genes in Arabidopsis.
authors.affiliation: Laboratory of Plant Breeding, Wageningen University, Graduate School of Experimental Plant Sciences, P.O. Box 386, 6700AJ, Wageningen, The Netherlands.
authors.forename: Benoit
authors.initials: B
authors.lastname: Gorguet
authors.forename: Danny
authors.initials: D
authors.lastname: Schipper
authors.forename: Adriaan W
authors.initials: AW
authors.lastname: van Heusden
authors.forename: Pim
authors.initials: P
authors.lastname: Lindhout
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics*; D035264:Flowers / Q000033:anatomy & histology* / Q000235:genetics; D017343:Genes, Plant / Q000502:physiology*; D008040:Genetic Linkage; D018551:Solanum lycopersicum / Q000235:genetics*; D018895:Microsatellite Repeats; D020161:Physical Chromosome Mapping; D051479:Plant Infertility / Q000235:genetics*; D011110:Polymorphism, Genetic; D026801:Synteny