Generating short peptidic ligands for silver nanowires from phage display random libraries.
We report the generation of peptide ligands for silver nanowires using a linear 12-mer peptide phage display random library technique. Phage clones that specifically bind the silver nanowires are sequenced after three rounds of biopanning, and obtained DNA sequences suggest that there are a few conserved amino acid residues which may be critical for binding. A selected binding peptide, together with two mutant peptide sequences, were subsequently synthesized on Tentagel resins to examine the importance of both the identities and positions of the conserved amino acid residues.
Chan
Priscilla
P
Department of Chemistry and Materials Science, Biosecurity and Nanosciences Laboratory, Lawrence Livermore National Laboratory, University of California, Livermore, CA 94551, USA.
Phan
Tiffany
T
Kao
Michael C
MC
Dolan
Cheryl
C
Tok
Jeffrey B-H
JB
D001483:Base Sequence; D001665:Binding Sites; D008024:Ligands; D043942:Nanotubes / Q000737:chemistry*; D010316:Particle Size; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013329:Structure-Activity Relationship
D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D012834:Silver