A magnetoelastic resonance biosensor immobilized with polyclonal antibody for the detection of Salmonella typhimurium.
Mass-sensitive, magnetoelastic resonance sensors have a characteristic resonant frequency that can be determined by monitoring the magnetic flux emitted by the sensor in response to an applied, time varying, magnetic field. This magnetostrictive platform has a unique advantage over conventional sensor platforms in that measurement is wireless and remote. A biosensor for the detection of Salmonella typhimurium was constructed by immobilizing a polyclonal antibody (the bio-molecular recognition element) onto the surface of a magnetostrictive platform. The biosensor was then exposed to solutions containing S. typhimurium bacteria. Binding between the antibody and antigen (bacteria) occurred and the additional mass of the bound bacteria caused a shift in the sensor's resonant frequency. Sensors with different physical dimensions were exposed to different concentrations of S. typhimurium ranging from 10(2) to 10(9)CFU/ml. Detection limits of 5x10(3) CFU/ml, 10(5) CFU/ml and 10(7) CFU/ml were obtained for sensors with the size of 2 mmx0.4 mmx15 microm, 5 mmx1 mmx15 microm and 25 mmx5 mmx15 microm, respectively. Good agreement between the measured number of bound bacterial cells (as measured by scanning electron microscopy (SEM)) and frequency shifts was obtained.
Guntupalli
R
R
Materials Research and Education center, Auburn University, Auburn, AL 36849, USA.
Hu
J
J
Lakshmanan
Ramji S
RS
Huang
T S
TS
Barbaree
James M
JM
Chin
Bryan A
BA
D000818:Animals; D000906:Antibodies; D015374:Biosensing Techniques / Q000295:instrumentation*; D008280:Magnetics / Q000295:instrumentation*; D011817:Rabbits; D012480:Salmonella Infections / Q000175:diagnosis* / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology / Q000302:isolation & purification*
D000906:Antibodies