Alpha-II-spectrin after controlled cortical impact in the immature rat brain.
Proteolytic processing plays an important role in regulating a wide range of important cellular functions, including processing of cytoskeletal proteins. Loss of cytoskeletal proteins such as spectrin is an important characteristic in a variety of acute central nervous system injuries including ischemia, spinal cord injury and traumatic brain injury (TBI). The literature contains extensive information on the proteolytic degradation of alpha-II-spectrin after TBI in the adult brain. By contrast, there is limited knowledge on the characteristics and relevance of these important processes in the immature brain. The present experiments examine TBI-induced proteolytic processing of alpha-II-spectrin after TBI in the immature rat brain. Distinct proteolytic products resulting from the degradation of the cytoskeletal protein alpha-II-spectrin by calpain and caspase 3 were readily detectable in cortical brain parenchyma and cerebrospinal fluid after TBI in immature rats.
Aikman
J
J
Center for Traumatic Brain Injury Studies, Evelyn F. & William L. McKnight Brain Institute of the University of Florida, Gainesville, FL, USA.
O'Steen
B
B
Silver
X
X
Torres
R
R
Boslaugh
S
S
Blackband
S
S
Padgett
K
K
Wang
K K W
KK
Hayes
R
R
Pineda
J
J
D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000134:cerebrospinal fluid; D001930:Brain Injuries / Q000134:cerebrospinal fluid* / Q000503:physiopathology; D002154:Calpain / Q000378:metabolism; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D016923:Cell Death / Q000502:physiology; D002540:Cerebral Cortex / Q000293:injuries* / Q000378:metabolism* / Q000473:pathology; D003599:Cytoskeleton / Q000378:metabolism*; D004195:Disease Models, Animal; D008279:Magnetic Resonance Imaging; D008297:Male; D009410:Nerve Degeneration / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D010446:Peptide Fragments / Q000134:cerebrospinal fluid; D051381:Rats; D017207:Rats, Sprague-Dawley; D013049:Spectrin / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology
D015415:Biomarkers; D010446:Peptide Fragments; D013049:Spectrin; D002154:Calpain; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases