title: Interleukin-10 and its role in clinical immunoparalysis following pediatric cardiac surgery.
abstract: OBJECTIVE
  A systemic insult is associated with subsequent hyporesponsiveness to endotoxin (as measured by ex vivo tumor necrosis factor [TNF]-alpha production) and an increased risk of late nosocomial infection in some patients. When combined with low monocyte surface major histocompatibility complex class II expression, this state of altered host defense is now commonly referred to as immunoparalysis. This study was undertaken to delineate the relationship between observed levels of the anti-inflammatory cytokine interleukin-10, common genetic polymorphisms that influence these levels, and the occurrence and severity of endotoxin hyporesponsiveness in children following elective cardiac surgery requiring cardiopulmonary bypass.
  
  
  METHODS
  A prospective observational clinical study.
  
  
  METHODS
  A tertiary pediatric cardiac center.
  
  
  METHODS
  Thirty-six infants and children <2 yrs of age undergoing elective cardiac surgery requiring cardiopulmonary bypass.
  
  
  METHODS
  None.
  
  
  RESULTS
  We investigated the production of TNF-alpha, interleukin-6, interleukin-8, interleukin-1 receptor antagonist, and interleukin-10 in whole blood in response to lipopolysaccharide (Neisseria meningitides 10 ng/mL) in samples drawn before, during, and up to 48 hrs after surgery. Patients were genotyped for the -1082, -819, and -592 interleukin-10 promoter polymorphisms. Whole blood cytokine response to lipopolysaccharide was reduced postoperatively to </=50% of preoperative levels for all cytokines measured. Stimulated cytokine production was lowest in cases with the highest postoperative plasma interleukin-10 levels, which were in turn associated with the GCC haplotype. Those patients in whom the whole blood response to endotoxin was maintained (TNF-alpha >100 pg/mL) over the first 48 hrs were more likely to have an uncomplicated short stay (odds ratio 4.7, 95% confidence interval 1-22).
  
  
  CONCLUSIONS
  Immediately following cardiac surgery, many children become relatively refractory to lipopolysaccharide stimulation. This immunoparalysis appears to be related in part to high circulating levels of interleukin-10 and places these patients at increased risk of postoperative complications. Interleukin-10 genotype may be a risk factor for immunoparalysis.
authors.affiliation: Critical Care Group-Portex Unit, Institute of Child Health, University College London, UK.
authors.forename: Meredith L
authors.initials: ML
authors.lastname: Allen
authors.forename: J Andreas
authors.initials: JA
authors.lastname: Hoschtitzky
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Peters
authors.forename: Martin
authors.initials: M
authors.lastname: Elliott
authors.forename: Allan
authors.initials: A
authors.lastname: Goldman
authors.forename: Ian
authors.initials: I
authors.lastname: James
authors.forename: Nigel J
authors.initials: NJ
authors.lastname: Klein
mesh_terms: D000704:Analysis of Variance; D006348:Cardiac Surgical Procedures / Q000009:adverse effects*; D016207:Cytokines / Q000097:blood; D005260:Female; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007108:Immune Tolerance / Q000235:genetics*; D066298:In Vitro Techniques; D007223:Infant; D016753:Interleukin-10 / Q000032:analysis / Q000097:blood* / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D018805:Sepsis / Q000209:etiology / Q000276:immunology*
chemical_list: D016207:Cytokines; D016753:Interleukin-10