title: Improved production of pristinamycin coupled with an adsorbent resin in fermentation by Streptomyces pristinaespiralis.
abstract: Batch fermentation by Streptomyces pristinaespiralis with the addition of adsorbent resins was used to increase the production of pristinamycin. In consideration of the adsorption capacity and the desorption ability, a polymeric resin, JD-1, was finally selected. The maximum production of pristinamycin in Erlenmeyer flasks went up to 1.13 from 0.4 g l(-1), by adding 12% (w/v) resin JD-1 into the culture broth at 20 h after inoculation. In a 3 l bioreactor, pristinamycin fermentation with the addition of 12% (w/v) resin JD-1 at 20 h after inoculation reached 0.8 g l(-1), which was a 1.25-fold increase over fermentation without resin.
authors.affiliation: Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou, 310027, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jia
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Jin
authors.forename: Yin-Lin
authors.initials: YL
authors.lastname: Lei
authors.forename: Le-He
authors.initials: LH
authors.lastname: Mei
authors.forename: Ning-Hui
authors.initials: NH
authors.lastname: Li
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001671:Biochemistry / Q000379:methods; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D025762:Pristinamycin / Q000096:biosynthesis* / Q000737:chemistry; D012116:Resins, Plant / Q000737:chemistry*; D013302:Streptomyces / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D025762:Pristinamycin; D012116:Resins, Plant