title: [Effect of transforming growth factor alpha on the expression of cyclin E and cyclin D1 in gastric carcinoma cells].
abstract: OBJECTIVE
  To explore the effect of transforming growth factor alpha (TGFalpha) on the expression of cyclin E and D1 in gastric carcinoma cells.
  
  
  METHODS
  Human gastric adenocarcinoma SGC7901 cells were cultured routinely and synchronized at G(0)/G(1) phase in serum-free RPMI-1640. The percentage of the cells at G(0)/G(1) phase was detected by propidium iodide staining and flow cytometry (FCM), and the synchronized cells were cultured in RPMI-1640 supplemented with 2.5% calf serum and treated with 10, 30, and 50 microg/L TGFalpha for 5 h. The expression of cyclin E and D1 in SGC7901 cells was detected by immunofluorescent staining and FCM.
  
  
  RESULTS
  The percentage of the cells at G(0)/G(1) phase increased from 54% in routine culture to 72% in the serum-free RPMI-1640 culture. TGFalpha treatment of the cells synchronized at G(0)/G(1) phase induced significant increment of cyclin E and D1 expressions (P<0.001), and at the dose of TGFalpha of 50 microg/L, their expressions increased by 25.18% and 27.52%, respectively (P<0.001).
  
  
  CONCLUSIONS
  TGFalpha can increase the expression of cyclin E and D1 in gastric carcinoma cells to promote their cell cycle progress.
authors.affiliation: Cancer Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lwj2@nfhoc.com
authors.forename: Wei-jiang
authors.initials: WJ
authors.lastname: Liang
authors.forename: Wan-dai
authors.initials: WD
authors.lastname: Zhang
authors.forename: Cun-long
authors.initials: CL
authors.lastname: Chen
authors.forename: Yong-li
authors.initials: YL
authors.lastname: Yao
authors.forename: Rong-cheng
authors.initials: RC
authors.lastname: Luo
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000096:biosynthesis*; D019927:Cyclin E / Q000096:biosynthesis*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology; D016211:Transforming Growth Factor alpha / Q000494:pharmacology*
chemical_list: D019927:Cyclin E; D016211:Transforming Growth Factor alpha; D019938:Cyclin D1