title: Relation between common polymorphisms in genes related to inflammatory response and colorectal cancer.
abstract: OBJECTIVE
  To investigate the association between common single nucleotide polymorphisms (SNPs) in inflammatory response-related genes such as interleukin (IL)-6, IL-8, tumor necrosis factor alpha (TNFalpha), peroxisome proliferators-activated receptor gamma (PPARgamma), intercellular adhesion molecule-1 (ICAM-1) and the risk of colorectal cancer (CRC) in a group of Greek patients.
  
  
  METHODS
  The study group consisted of 222 CRC patients and 200 healthy controls. Genotyping was performed using allele-specific PCR of PRC-RFLP and the results were confirmed by sequencing. We studied the association of SNPs in the IL-6 (-174G>C), IL-8 (-251T>A), TNFalpha (-308G>A), ICAM-1 (R241G and K469E), and PPARgamma (Pro12Ala) genes and the risk of CRC.
  
  
  RESULTS
  The IL-6 -174G, R241 and K469 alleles of ICAM-1 were associated with increased risk of CRC (OR = 1.77, 95% CI: 1.34-2.34; OR = 1.83, 95% CI: 1.23-2.72; and OR = 1.35, 95% CI: 1.03-1.77 respectively). The IL-8 and TNFalpha polymorphisms had no effect. Whereas the PPARgamma Pro12 genotype was associated with increased risk of disease (OR = 1.78, 95% CI: 1.25-2.49).
  
  
  CONCLUSIONS
  The association between common SNPs in immunologic response-related genes and CRC is reported in the present study. Apart from shedding light on the mechanisms of malignancy initiation and progression, SNPs may improve appropriate screening for sub-populations at risk.
authors.affiliation: 1st Propaideutic Surgical Department, Hippocration University Hospital, University of Athens, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Theodoropoulos
authors.forename: Ioannis
authors.initials: I
authors.lastname: Papaconstantinou
authors.forename: Evangelos
authors.initials: E
authors.lastname: Felekouras
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Nikiteas
authors.forename: Petros
authors.initials: P
authors.lastname: Karakitsos
authors.forename: Dimitris
authors.initials: D
authors.lastname: Panoussopoulos
authors.forename: Andreas Ch
authors.initials: ACh
authors.lastname: Lazaris
authors.forename: Efstratios
authors.initials: E
authors.lastname: Patsouris
authors.forename: John
authors.initials: J
authors.lastname: Bramis
authors.forename: Maria
authors.initials: M
authors.lastname: Gazouli
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D005838:Genotype; D006115:Greece; D006801:Humans; D007249:Inflammation / Q000235:genetics* / Q000473:pathology*; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D047495:PPAR gamma / Q000235:genetics; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D047495:PPAR gamma; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1