Regenerative capacity of individual liver lobes in the microsurgical mouse model.
In the development of microsurgical mouse models of hepatic regeneration and repair, lobe-specific regenerative responses were observed. We therefore determined the hepatic regenerative capacity of individual mouse liver lobes. In mice, 26, 60, 75, and 83% of total liver mass was resected. Bromo-deoxyuridine (BrdU) was injected prior to liver harvest and the BrdU labeling index determined in all remaining individual liver lobes. BrdU-positive nuclei were seen in all liver lobes after the 26 and 60% resection, but significantly fewer were detected in the caudate lobe. In the 75% group, equally distributed positive nuclei were found. However, BrdU labeling was scant in the 83% group. In microsurgical mouse liver-regeneration models, the average hepatic response depends on amount of liver tissue resected and on the remaining liver lobe. BrdU incorporation can vary significantly among individual lobes. The lobe-specific differences observed may prove valuable in further investigations of hepatic regeneration and repair.
Inderbitzin
Daniel
D
Department of Visceral and Transplantation Surgery, University Hospital Bern, Bern, Switzerland. daniel.inderbitzin@insel.ch
Studer
Peter
P
Sidler
Daniel
D
Beldi
Guido
G
Djonov
Valentin
V
Keogh
Adrian
A
Candinas
Daniel
D
D000818:Animals; D005500:Follow-Up Studies; D006498:Hepatectomy / Q000379:methods*; D008099:Liver / Q000502:physiology / Q000601:surgery / Q000648:ultrastructure; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D008866:Microsurgery / Q000379:methods*; D009929:Organ Size / Q000502:physiology