The interaction of the polyphenylacetylene surface with biological environments studied by XPS, RAIRS and biological tests.
A pi-conjugated polymer, polyphenylacetylene or PPA, has been tested for its possible applications as biosensor or biomaterial. Protein adsorption was investigated by incubating PPA films in solutions of bovine serum albumin (BSA) dissolved in phosphate buffer (PBS) having increasing protein concentration. Investigations on the PPA films were carried out by means of two surface analysis techniques, X-ray photoelectron spectroscopy (XPS) and reflection-absorption infrared spectroscopy (RAIRS). Desorption of BSA from the PPA surface was also investigated. Finally, the cytototoxicity of the PPA surface was checked by measuring viability and proliferation of lymphoma macrophages and SAOS osteoblasts grown in the presence of the polymer.
Iucci
G
G
Dipartimento di Fisica E. Amaldi, Universit à Roma Tre, Unità INFM, INSTM, and CISDiC, Via della Vasca Navale 84, 00146, Roma. iucci@uniroma3.it
Rossi
L
L
Rosato
N
N
Savini
I
I
Duranti
G
G
Polzonetti
G
G
D000114:Acetylene / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D008223:Lymphoma / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013052:Spectrometry, X-Ray Emission / Q000379:methods*; D013499:Surface Properties; D020298:U937 Cells
D001672:Biocompatible Materials; D012710:Serum Albumin, Bovine; D000114:Acetylene