RIVETS: the recombinant immunoglobulin and viral epitope tag system.
The human monoclonal antibody 8F9 binds to a linear 10 amino acid epitope that is present within the N-terminal region of the gB envelope glycoprotein of HCMV. Here we show that this short sequence (ETIYNTTLKY) can function as a tag for the detection of recombinant proteins using antibody 8F9. The AD-2S1 tag was recognized by 8F9 whether present at the N- or C-terminus of recombinant proteins and tagged recombinant proteins could be quantified with multiple analytical techniques such as ELISA, western blotting, immunofluorescence and flow cytometry. Production of 8F9 using different constant regions or constant regions from different species enhances the convenience and range of use of this system which we term the Recombinant Immunoglobulin and Viral Epitope Tag System or RIVETS.
McLean
Gary R
GR
The Biomedical Research Centre, University of British Columbia, 2222 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3. Gary.Mclean@uth.tmc.edu
Cho
Chin-wen
CW
Trotter
Brendon
B
Schrader
John W
JW
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D000915:Antibody Affinity; D000956:Antigens, Viral / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics* / Q000276:immunology; D047468:Immunoprecipitation; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C080634:human cytomegalovirus antigen p55-52