Occurrence of two different types of RNA-5-containing beet necrotic yellow vein virus in the UK.
Two types of RNA-5-containing beet necrotic yellow vein virus (BNYVV) have been detected in the UK at different sites in Norfolk. On the basis of nucleotide (nt) sequence comparisons, one virus source (UK-MH) was clearly identified as P type BNYVV, a virus type that had previously only been detected in two widely separated parts of the world, France and Kazakhstan. The other virus source (UK-FF) has a complex genome composition. The analysed portions of its RNAs 2 and 4 are closely related to the corresponding portions in the RNAs of the East Asian A type isolate S, whereas those of its RNAs 1 and 3 resemble P type RNA 1 from Kazakhstan and European A type RNA 3, respectively. Interestingly, the P25 encoded on its RNA 3 has an unique TYHG tetrad in the highly variable amino acid positions 67-70. RNA 5 of the UK-FF BNYVV source shares properties with P type RNA 5, but also with East Asian types of RNA 5. The possible origin and epidemiology of BNYVV types is discussed.
Ward
L
L
Central Science Laboratory, Sand Hutton, York, UK.
Koenig
R
R
Budge
G
G
Garrido
C
C
McGrath
C
C
Stubbley
H
H
Boonham
N
N
D001483:Base Sequence; D027461:Beta vulgaris / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D005060:Europe; D007623:Kazakhstan; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D006113:United Kingdom
D017931:DNA Primers; D012367:RNA, Viral