Functional reconstitution of the human epithelial Na+ channel in a mammalian expression system.
Probing ion channel structure-function and regulation in native tissue can, in some instances, be experimentally challenging or impractical. To facilitate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na+ channel heterologously expressed in Chinese hamster ovary cells.
Staruschenko
Alexander
A
Department of Physiology, University of Texas Health Science Center at San Antonio, USA.
Booth
Rachell E
RE
Pochynyuk
Oleh
O
Stockand
James D
JD
Tong
Qiusheng
Q
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D053503:Epithelial Sodium Channels / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D018408:Patch-Clamp Techniques; D011994:Recombinant Proteins / Q000378:metabolism
D053503:Epithelial Sodium Channels; D011994:Recombinant Proteins