title: Dose-dependent effect of low-intensity pulsed ultrasound on callus formation during rapid distraction osteogenesis.
abstract: Distraction osteogenesis of bone or callotasis causes poor bone formation when the distraction rate is beyond the optimal rate. Low-intensity pulsed ultrasound was reported to enhance fracture healing, treatment of nonunion, and accelerate bone maturation and remodeling during consolidation stage of distraction osteogenesis. In this study, we evaluated the efficacy of different durations of LIPUS treatments during rapid bone lengthening. After 7-day latent period, osteotomized New Zealand white rabbit tibiae were lengthened at the rate of 2 mm per day for 1 week. Two different LIPUS treatment durations of 20 min and 40 min were selected for treatment groups. Rabbits without treatment served as the control group. Plain X-ray, peripheral quantitative computed tomography (pQCT) and histology were performed to assess bone acquisition in the distraction callus. The results showed that LIPUS increased bone mineral content and volume of the mineralized tissue of distraction callus in a dose-dependent manner. The different regions of distraction callus exhibited various spatial response to LIPUS treatment. Moreover, LIPUS enhanced dose-dependant endochondral formation. Compared with 20-min treatment, the 40-min LIPUS treatment was a more favorable treatment duration for bone regeneration in the distraction callus. In conclusion, LIPUS was able to enhance bone regeneration under rapid distraction, and its effect was dose-dependent.
authors.affiliation: Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
authors.forename: Chun Wai
authors.initials: CW
authors.lastname: Chan
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
authors.forename: Kwong Man
authors.initials: KM
authors.lastname: Lee
authors.forename: Wing Hoi
authors.initials: WH
authors.lastname: Cheung
authors.forename: Jack Chun Yiu
authors.initials: JC
authors.lastname: Cheng
authors.forename: Kwok Sui
authors.initials: KS
authors.lastname: Leung
mesh_terms: D000818:Animals; D016723:Bone Remodeling; D002146:Bony Callus / Q000000981:diagnostic imaging* / Q000473:pathology; D004195:Disease Models, Animal; D019857:Osteogenesis, Distraction; D010027:Osteotomy; D011817:Rabbits; D013977:Tibia / Q000000981:diagnostic imaging* / Q000378:metabolism; D013997:Time Factors; D014057:Tomography, X-Ray Computed; D014464:Ultrasonic Therapy; D014463:Ultrasonography