title: Microarray profiling of skeletal muscle sarcoplasmic reticulum proteins.
abstract: Microarrays were developed to profile the level of proteins associated with calcium regulation in sarcoplasmic reticulum (SR) isolated from porcine Longissimus muscle. The microarrays consisted of SR preparations printed onto to glass slides and probed with monoclonal antibodies to 7 target proteins. Proteins investigated included: ryanodine receptor, (RyR), dihydropyridine receptor, (DHPR), triadin (TRI), calsequestrin (CSQ), 90 kDa junctional protein (JSR90), and fast-twitch and slow-twitch SR calcium ATPases (SERCA1 and SERCA2). Signal from a fluorescently-labeled detection antibody was measured and quantitated using a slide reader. The microarray developed was also employed to profile Longissimus muscle SR proteins from halothane genotyped animals. Significant (P<0.05) reductions in levels of several proteins were found including: RyR, CSQ, TRI, DHPR and SERCA2 in SR samples from halothane positive animals. The results illustrate the potential of microarrays as a tool for profiling SR proteins and aiding investigations of calcium regulation.
authors.affiliation: Department of Animal Science, University of Nebraska, Lincoln, 68583, USA.
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Schulz
authors.forename: Nathan
authors.initials: N
authors.lastname: Palmer
authors.forename: Jon
authors.initials: J
authors.lastname: Steckelberg
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Jones
authors.forename: Michael G
authors.initials: MG
authors.lastname: Zeece
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling / Q000379:methods*; D015151:Immunoblotting; D009124:Muscle Proteins / Q000032:analysis*; D040081:Protein Array Analysis / Q000379:methods*; D012519:Sarcoplasmic Reticulum / Q000737:chemistry*; D013552:Swine
chemical_list: D009124:Muscle Proteins