[Application of EB virus latent membrane protein 1 in nasopharyngeal swab in diagnosis of nasopharyngeal carcinoma].
OBJECTIVE
To investigate the feasibility and reliability of detection of Epstein-Barr virus (EBV) latent membrane protein-1 (LMP1) gene by nasopharyngeal swab in the diagnosis of nasopharyngeal carcinoma (NPC). To investigate the distribution of 30 bp deletion variant of LMP-1 gene in the local population.


METHODS
Nasopharyngeal cells were collected by nasopharyngeal swab, and then DNA was extracted, which was subsequently confirmed by amplification of sequence of beta-thalassemia gene by polymerase chain reaction (PCR). And sequence of LMP1 was amplified with specific primer to verify the significance of LMP1 in the diagnosis of NPC.


RESULTS
DNA was obtained from 96.4% nasopharyngeal swab samples, LMP1 was detected in 33 of 36 samples, while 2 of 45 samples from normal control, with sensitivity 91.7%, and specialty 95.6%. 30 bp deletion of LMP1 gene was found in 80.6% of NPC samples, and wild type 11.1%.


CONCLUSIONS
Our study suggests that nasopharyngeal swab could be effective method for gene-detection. As a parameter in diagnosis of NPC, LMP1 gene is maybe superior to EBVCA-IgA. 30 bp deletion of LMP1 oncogene is widespread in NPC patients.
Xie
Ying
Y
Department of Otolaryngology, the First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.
Huang
Guangwu
G
Zhang
Zhe
Z
Wen
Wensheng
W
Wu
Yingying
Y
Wei
Zhengbo
Z
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004279:DNA, Viral; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis* / Q000473:pathology; D012680:Sensitivity and Specificity; D017384:Sequence Deletion; D014763:Viral Matrix Proteins / Q000032:analysis* / Q000235:genetics; D055815:Young Adult
D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins