High-hydrostatic-pressure treatment impairs actin cables and budding in Saccharomyces cerevisiae.
We previously reported that the postgrowth of Escherichia coli K-12 after high-hydrostatic-pressure treatment (HPT) as moderate as 75 MPa for 30 min at 37 degrees C induced the formation of elongated cells due to an HPT-induced disorder in FtsZ ring formation, which is essential for cell division. Because an FtsZ ring is known as a bacterial cytoskeleton, we examined the effect of HPT on a eukaryotic cytoskeleton, such as actin cables (long bundles of actin filaments), of Saccharomyces cerevisiae. We found that actin cables disappeared after HPT (100 MPa) and were not reorganized until 3.5 h of growth after HPT. As long as actin cables disappeared, budding did not start. We also demonstrated that the in vitro polymerization of actin monomers was highly sensitive to HPT.
Kawarai
Taketo
T
Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
Arai
Seisuke
S
Furukawa
Soichi
S
Ogihara
Hirokazu
H
Yamasaki
Makari
M
D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003599:Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure*; D040542:Mechanotransduction, Cellular / Q000502:physiology; D011312:Pressure; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000502:physiology*
D000199:Actins