The role of N-lauroylethanolamine in the regulation of senescence of cut carnations (Dianthus caryophyllus).
N-acylethanolamines (NAEs) are a group of lipid mediators that play important roles in mammals, but not much is known about their precise function in plants. In this work, we analyzed the possible involvement of N-lauroylethanolamine [NAE(12:0)] in the regulation of cut-flower senescence. In cut carnation flowers of cv. Red Barbara, the pulse treatment with 5 microM NAE(12:0) slowed senescence by delaying the onset of initial wilting. Ion leakage, which is a reliable indicator of membrane integrity, was postponed in NAE(12:0)-treated flowers. The lipid peroxidation increased in carnation petals with time, in parallel to the development in activity of lipoxygenase and superoxide anion production rate, and these increases were both delayed by NAE(12:0) supplementation. The activities of four enzymes (superoxide dismutase, catalase, glutathione reductase and ascorbate peroxidase) that are implicated in antioxidant defense were also upregulated in the cut carnations that had been treated with NAE(12:0). These data indicate that NAE(12:0)-induced delays in cut-carnation senescence involve the protection of the integrity of membranes via suppressing oxidative damage and enhancing antioxidant defense. We propose that the stage from the end of blooming to the onset of wilting is a critical period for NAE(12:0) action.
Zhang
Yun
Y
College of Life Science, Nanjing Agricultural University, Nanjing 210095, China.
Guo
Wei-ming
WM
Chen
Su-mei
SM
Han
Liang
L
Li
Zheng-ming
ZM
D000375:Aging; D000975:Antioxidants / Q000378:metabolism; D060387:Ascorbate Peroxidases; D002374:Catalase / Q000378:metabolism; D029749:Dianthus / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D004983:Ethanolamines / Q000378:metabolism* / Q000494:pharmacology; D035264:Flowers / Q000201:enzymology / Q000502:physiology; D005980:Glutathione Reductase / Q000378:metabolism; D007700:Kinetics; D007850:Lauric Acids / Q000378:metabolism* / Q000494:pharmacology; D008084:Lipoxygenase / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D004983:Ethanolamines; D007850:Lauric Acids; D010940:Plant Proteins; D013481:Superoxides; C090359:N-lauroylethanolamine; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D008084:Lipoxygenase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase