RNA extraction for arrays.
DNA microarrays enable insights into global gene expression by capturing a snapshot of cellular expression levels at the time of sample collection. Careful RNA handling and extraction are required to preserve this information properly, ensure sample-to-sample reproducibility, and limit unwanted technical variation in experimental data. This chapter discusses important considerations for "array-friendly" sample handling and processing from biosamples such as blood, formalin-fixed, paraffin-embedded samples, and fresh or flash-frozen tissues and cells. It also provides guidelines on RNA quality assessments, which can be used to validate sample preparation and maximize recovery of relevant biological information.
Madabusi
Lakshmi V
LV
Asuragen Discovery Services, Asuragen, Inc., Austin, TX, USA.
Latham
Gary J
GJ
Andruss
Bernard F
BF
D000818:Animals; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000097:blood* / Q000302:isolation & purification*
D012313:RNA