title: [Polymorphism of D2S1338 and D19S433 loci in Southern Chinese Han nationality population and its application].
abstract: This study was aimed to investigate the polymorphism of D2S1338 and D19S433 loci in Southern Chinese Han nationality population, and to evaluate its forensic application. The DNA was extracted by chromatography on che-lex-100. Fifteen STR loci (D2S1338, D19S433 etc.) were amplified by Identifiler kit and analyzed by 3100 genetic analyzer. The softwares of analysis were GeneScan 3.0 and Genetype 3.7. The results indicated that the allele frequencies of H, DP, PIC, PE of D2S1338 and D19S433 loci were obtained. The allele frequency of samples were in Hardy-Weinberg equilibrium by chi2 test. No mutation was found on D2S1338 and D19S433 loci in 370 cases of meiosis. It is concluded that the D2S1338 and D19S433 loci are high polymorphic and their mutation rates in Southern Chinese Han nationality population are low. Their good distribution is suitable for forensic individual identification and paternity testing.
authors.affiliation: Department of Medicel Laboratory Examinations, Longgang Women and Children Health Hospital, Shenzhen 518172, China. tantan_baby@hotmail.com
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Deng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Guo-Guang
authors.initials: GG
authors.lastname: Wu
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D016172:DNA Fingerprinting / Q000379:methods; D005554:Forensic Medicine / Q000379:methods*; D005787:Gene Frequency; D014644:Genetic Variation; D006801:Humans; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences