Permissibility of different cell types for the growth of avian metapneumovirus.
Vero cells are commonly used for the growth of avian metapneumovirus subtype C (aMPV-C). This study was conducted to evaluate 17 different cell types for the growth of a Minnesota strain of aMPV-C. The virus was inoculated into these cell types and virus growth was monitored by the development of cytopathic effects (cpe) and immunofluorescence. Virus growth was obtained in 6 of 17 cell types tested with the highest virus titers observed in BGM and DF-1 cells. The flow cytometric analysis of cells at 72 h post inoculation found the highest number of infected cells in BGM cells followed by QT-35 cells. At 48 h post inoculation, DF-1 and BGM cells showed the highest number of infected cells. These results suggest that BGM, QT-35, and DF-1 cells can be used for high titer propagation of aMPV-C.
Tiwari
A
A
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, USA.
Patnayak
Devi P
DP
Chander
Yogesh
Y
Goyal
Sagar M
SM
D000818:Animals; D002415:Cats; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured / Q000821:virology*; D002645:Chickens; D002522:Chlorocebus aethiops; D003370:Coturnix; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D004285:Dogs; D005455:Fluorescent Antibody Technique; D006736:Horses; D029121:Metapneumovirus / Q000254:growth & development*; D051379:Mice; D008907:Mink; D011817:Rabbits; D013552:Swine; D014422:Turkeys; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation
D014764:Viral Proteins