Circadian intraocular pressure rhythm is generated by clock genes.
OBJECTIVE
The present study in a mouse model was undertaken to reveal the role of the circadian clock genes Cry1 and Cry2 in generation of 24-hour intraocular pressure (IOP) rhythm.


METHODS
IOP was measured at eight time points daily (circadian time [CT] 0, 3, 6, 9, 12, 15, 18, and 21 hours), using a microneedle method in four groups of C57BL/6J mice (groups 1 and 3, wild-type; groups 2 and 4, Cry-deficient [Cry1-/-Cry2-/-]). During the IOP measurements, mice in groups 1 and 2 were maintained in a 12-hour light-dark cycle (LD), whereas mice in groups 3 and 4 were kept in a constant darkness (DD) that started 24 to 48 hours before the measurements. Circadian IOP variations in each group were evaluated by one-way analysis of variance (ANOVA) and Scheffé tests.


RESULTS
In wild-type mice living in LD conditions, pressures measured in the light phase were significantly lower than those in the dark phase. This daily rhythm was maintained under DD conditions with low pressure in the subjective day and high pressure in the subjective night. In contrast, Cry-deficient mice did not show significant circadian changes in IOP, regardless of environmental light conditions.


CONCLUSIONS
These findings demonstrate that clock oscillatory mechanisms requiring the activity of core clock genes are essential for the generation of a circadian rhythm of intraocular pressure.
Maeda
Ari
A
Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Ishikawa, Japan. ari@p2222.nsk.ne.jp
Tsujiya
Sosuke
S
Higashide
Tomomi
T
Toida
Kazunori
K
Todo
Takeshi
T
Ueyama
Tomoko
T
Okamura
Hitoshi
H
Sugiyama
Kazuhisa
K
D000818:Animals; D001683:Biological Clocks; D056926:CLOCK Proteins; D002940:Circadian Rhythm / Q000502:physiology*; D056931:Cryptochromes; D003623:Dark Adaptation; D005420:Flavoproteins / Q000502:physiology*; D007429:Intraocular Pressure / Q000502:physiology*; D008027:Light; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015534:Trans-Activators / Q000502:physiology*
C538964:Cry1 protein, mouse; C538967:Cry2 protein, mouse; D056931:Cryptochromes; D005420:Flavoproteins; D015534:Trans-Activators; D056926:CLOCK Proteins; C538944:Clock protein, mouse