Live cell refractometry using microfluidic devices.
Using Hilbert phase microscopy for extracting quantitative phase images, we measured the average refractive index associated with live cells in culture. To decouple the contributions to the phase signal from the cell refractive index and thickness, we confined the cells in microchannels. The results are confirmed by comparison with measurements of spherical cells in suspension.
Lue
Niyom
N
G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Popescu
Gabriel
G
Ikeda
Takahiro
T
Dasari
Ramachandra R
RR
Badizadegan
Kamran
K
Feld
Michael S
MS
D048430:Cell Shape; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques; D008858:Microscopy, Phase-Contrast; D012031:Refractometry / Q000295:instrumentation