Probing the effects of phosphoinositides on ion channels.
Ion channels are integral membrane proteins that control transmembrane ion fluxes to regulate membrane potential, cell excitability, and ion transport. Membrane phospholipids containing phosphoinositides have recently emerged as important regulators of many ion channels, including inward rectifier K+ channel, voltage-gated K+ and Ca2+ channels, transient receptor potential channels, and intracellular inositol-1,4,5-trisphosphate receptor ion channels. Discussed here are several methods for studying regulation of ion channels by phospholipids.
Huang
Chou-Long
CL
Department of Medicine, UT Southwestern Medical Center, Dallas, TX, USA.
D000818:Animals; D016466:CHO Cells; D002417:Cattle; D006224:Cricetinae; D003412:Cricetulus; D007473:Ion Channels / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism* / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism
D007473:Ion Channels; D019269:Phosphatidylinositol 4,5-Diphosphate; D011956:Receptors, Cell Surface; D010738:Type C Phospholipases