Enhanced antisense effect of modified PNAs delivered through functional PMMA microspheres.
Peptide nucleic acids (PNA) are very promising antisense agents, but their in vivo application is often hampered by their low bioavailability, mainly due to their limited uptake through cellular and nuclear membranes. However, PNA chemical synthesis easily allows modification with functional structures able to improve the intrinsically low permeability and great interest is arising in finding specific and efficient delivery protocols. Polymeric core-shell microspheres with anionic functional groups on the surface were tested for their ability to reversibly bind lysine modified PNA sequences, whose antisense activity against COX-2 mRNA was already demonstrated in murine macrophages.
Chiarantini
Laura
L
Institute of Biological Chemistry, University of Urbino, Italy. l.chiarantini@uniurb.it
Cerasi
Aurora
A
Millo
Enrico
E
Sparnacci
Katia
K
Laus
Michele
M
Riccio
Massimo
M
Santi
Spartaco
S
Ballestri
Marco
M
Spaccasassi
Silvia
S
Tondelli
Luisa
L
D000180:Acrylic Resins; D000818:Animals; D016375:Antisense Elements (Genetics); D001682:Biological Availability; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000235:genetics; D005079:Excipients; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010316:Particle Size; D020135:Peptide Nucleic Acids / Q000008:administration & dosage* / Q000494:pharmacology* / Q000633:toxicity; D019904:Polymethyl Methacrylate; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013816:Thermodynamics
D000180:Acrylic Resins; D016375:Antisense Elements (Genetics); C446821:Eudragit L100-55; D005079:Excipients; D008070:Lipopolysaccharides; D020135:Peptide Nucleic Acids; D012333:RNA, Messenger; D019904:Polymethyl Methacrylate; D051546:Cyclooxygenase 2