Discrimination of basal cell carcinoma from normal dermal stroma by quantitative multiphoton imaging.
We performed multiphoton fluorescence (MF) and second-harmonic generation (SHG) imaging on human basal cell carcinoma samples. In the dermis, basal cell carcinomas can be identified by masses of autofluorescent cells with relatively large nuclei and marked peripheral palisading. In the normal dermis, SHG from dermal collagen contributes largely to the multiphoton signal. However, within the cancer stroma, SHG signals diminish and are replaced by autofluorescent signals, indicating that normal collagen structures responsible for SHG have been altered. To better delineate the cancer cells and cancer stroma from the normal dermis, a quantitative MF to SHG index is developed. We demonstrate that this index can be used to differentiate cancer cells and adjacent cancer stroma from the normal dermis. Our work shows that MF and SHG imaging can be an alternative for Mohs' surgery in the real-time guidance of the secure removal of basal cell carcinoma.
Lin
Sung-Jan
SJ
Department of Dermatology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Jee
Shiou-Hwa
SH
Kuo
Chien-Jui
CJ
Wu
Ruei-Jr
RJ
Lin
Wei-Chou
WC
Chen
Jau-Shiuh
JS
Liao
Yi-Hua
YH
Hsu
Chih-Jung
CJ
Tsai
Tsen-Fang
TF
Chen
Yang-Fang
YF
Dong
Chen-Yuan
CY
D002280:Basal Cell Carcinoma / Q000175:diagnosis* / Q000473:pathology / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D020405:Dermis / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000648:ultrastructure; D017154:Stromal Cells / Q000648:ultrastructure*