title: Solid pseudopapillary neoplasms of the pancreas are associated with FLI-1 expression, but not with EWS/FLI-1 translocation.
abstract: Solid pseudopapillary neoplasms of the pancreas are rare pancreatic tumors with mostly benign behavior, affecting almost exclusively women. Their histogenetic origin is still unsolved, but a recently reported EWS/FLI-1 translocation t(11;22)(q24;q21) and the consistent expression of CD56 and the progesterone receptor, both genes located on the long arm of chromosome 11, point to chromosome 11q as a potential locus of gene aberration in solid pseudopapillary neoplasms. To further elucidate this issue, we studied 30 cases of solid pseudopapillary neoplasms by comparative genomic hybridization (CGH), fluorescent in situ hybridization (FISH) and immunohistochemistry. Immunohistochemically, 38% showed nuclear expression of FLI-1 and all cases revealed positivity for CD56 and the progesterone receptor, whereas no solid pseudopapillary neoplasm expressed CD34. No translocation of the EWS gene was found by FISH and no gross chromosomal gain or loss was detected by CGH. It is concluded that FLI-1 expression in solid pseudopapillary neoplasms is not associated with an EWS/FLI-1 translocation. In addition, there are no chromosomal gains or losses, especially on chromosome 11, where the FLI-1 gene is located adjacent to the gene for CD56 (NCAM). These data add another feature to the complex phenotypic appearance of solid pseudopapillary neoplasms.
authors.affiliation: Department of Pathology, University of Kiel, Kiel, Germany. k.peters@path.uni-kiel.de
authors.forename: Katharina
authors.initials: K
authors.lastname: Tiemann
authors.forename: Markus
authors.initials: M
authors.lastname: Kosmahl
authors.forename: Julia
authors.initials: J
authors.lastname: Ohlendorf
authors.forename: Matthias
authors.initials: M
authors.lastname: Krams
authors.forename: Günter
authors.initials: G
authors.lastname: Klöppel
mesh_terms: D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D019002:CD56 Antigen / Q000032:analysis; D002648:Child; D002864:Chromogranins / Q000032:analysis; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D056741:Cyclin D; D016213:Cyclins / Q000032:analysis; D003937:Diagnosis, Differential; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008840:Microfilament Proteins / Q000032:analysis / Q000235:genetics*; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D034802:RNA-Binding Protein EWS / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear / Q000032:analysis / Q000235:genetics*; D016708:Synaptophysin / Q000032:analysis; D015534:Trans-Activators; D014178:Translocation, Genetic; D051176:beta Catenin / Q000032:analysis
chemical_list: D018952:Antigens, CD34; D019002:CD56 Antigen; D002864:Chromogranins; D056741:Cyclin D; D016213:Cyclins; C098179:FLII protein, human; D008840:Microfilament Proteins; D034802:RNA-Binding Protein EWS; D018160:Receptors, Cytoplasmic and Nuclear; D016708:Synaptophysin; D015534:Trans-Activators; D051176:beta Catenin