title: Possible role of preproghrelin gene polymorphisms in susceptibility to bulimia nervosa.
abstract: Previous investigations have suggested that ghrelin, an endogenous orexigenic peptide, is involved in the pathology of eating disorders. We conducted a study to determine whether any preproghrelin gene polymorphisms are associated with eating disorders. Three hundred thirty-six eating disorder patients, including 131 anorexia nervosa (AN)-restricting types (AN-R), 97 AN-binge eating/purging types (AN-BP) and 108 bulimia nervosa (BN)-purging types (BN-P), and 300 healthy control subjects participated in the study. Genotyping was performed to determine the polymorphisms present, and with this information, linkage disequilibrium (LD) between the markers was analyzed and the distributions of the genotypes, the allele frequencies, and the haplotype frequencies were compared between the groups. The Leu72Met (408 C > A) (rs696217) polymorphism in exon 2 and the 3056 T > C (rs2075356) polymorphism in intron 2 were in LD (D' = 0.902, r2 = 0.454). Both polymorphisms were significantly associated with BN-P (allele-wise: P = 0.0410, odds ratio (OR) = 1.48; P = 0.0035, OR = 1.63, for Leu72Met and 3056 T > C, respectively). In addition, we observed a significant increase in the frequency of the haplotype Met72-3056C in BN-P patients (P = 0.0059, OR = 1.71). Our findings suggest that the Leu72Met (408 C > A) and the 3056 T > C polymorphisms of the preproghrelin gene are associated with susceptibility to BN-P.
authors.affiliation: Department of Psychosomatic Research, National Institute of Mental Health, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan. ando@ncnp-k.go.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ando
authors.forename: Gen
authors.initials: G
authors.lastname: Komaki
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Naruo
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Okabe
authors.forename: Masato
authors.initials: M
authors.lastname: Takii
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kawai
authors.forename: Fujiko
authors.initials: F
authors.lastname: Konjiki
authors.forename: Michiko
authors.initials: M
authors.lastname: Takei
authors.forename: Takakazu
authors.initials: T
authors.lastname: Oka
authors.forename: Kaori
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Akinori
authors.initials: A
authors.lastname: Masuda
authors.forename: Norio
authors.initials: N
authors.lastname: Ozaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Suematsu
authors.forename: Kenzo
authors.initials: K
authors.lastname: Denda
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kurokawa
authors.forename: Kotarou
authors.initials: K
authors.lastname: Itakura
authors.forename: Chikara
authors.initials: C
authors.lastname: Yamaguchi
authors.forename: Masaki
authors.initials: M
authors.lastname: Kono
authors.forename: Tatsuyo
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Nakai
authors.forename: Aya
authors.initials: A
authors.lastname: Nishizono-Maher
authors.forename: Masanori
authors.initials: M
authors.lastname: Koide
authors.forename: Ken
authors.initials: K
authors.lastname: Murakami
authors.forename: Kiyohide
authors.initials: K
authors.lastname: Nagamine
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Tomita
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ookuma
authors.forename: Kazumi
authors.initials: K
authors.lastname: Tomita
authors.forename: Eita
authors.initials: E
authors.lastname: Tonai
authors.forename: Akira
authors.initials: A
authors.lastname: Ooshima
authors.forename: Toshio
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Ichimaru
mesh_terms: D000328:Adult; D052018:Bulimia Nervosa / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D054439:Ghrelin; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium; D036361:Peptide Hormones / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D054439:Ghrelin; D036361:Peptide Hormones