title: Decreased growth rate of lung metastatic lesions after splenectomy in mice.
abstract: BACKGROUND
  The effect of splenectomy on hematogenous metastases of malignant tumors remains controversial. The aim of this study was to clarify the effect of splenectomy on hematogenous metastases in an animal model.
  
  
  METHODS
  Colon 26 cancer cells were inoculated into the lateral tail vein of 90 mice. The mice were then assigned to a splenectomy group or a control group. Lung weight, number of lung metastases, size of metastatic nodules, and serum vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) levels after the surgical procedure were measured and compared between the two groups.
  
  
  RESULTS
  The lung nodules were significantly smaller in the splenectomy group than in the control group. In both groups, the serum VEGF levels increased on day 1 and then decreased. The serum VEGF levels on day 5 were significantly lower in the splenectomy group than in the control group. The serum bFGF levels were significantly lower in the splenectomy group than in the control group on days 1 and 5. Immunohistochemical study showed that bFGF was produced by reticuloendothelial cells of the spleen.
  
  
  CONCLUSIONS
  The growth rate of hematogenous metastatic lesions appears to decrease after splenectomy and may be associated with decreases in serum levels of VEGF and bFGF induced by splenectomy.
authors.affiliation: Department of Surgery 1, Oita University Faculty of Medicine, Yufu, Oita, Japan. surgkazuson@mac.com
authors.forename: Kazuya
authors.initials: K
authors.lastname: Sonoda
authors.forename: Koichi
authors.initials: K
authors.lastname: Izumi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsui
authors.forename: Masafumi
authors.initials: M
authors.lastname: Inomata
authors.forename: Norio
authors.initials: N
authors.lastname: Shiraishi
authors.forename: Seigo
authors.initials: S
authors.lastname: Kitano
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000473:pathology; D005260:Female; D005346:Fibroblast Growth Factors / Q000378:metabolism; D008175:Lung Neoplasms / Q000473:pathology* / Q000556:secondary / Q000601:surgery*; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis / Q000628:therapy; D013156:Splenectomy; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A; C108111:fibroblast growth factor 13; D005346:Fibroblast Growth Factors