title: First report of bluetongue virus serotype 1 isolated from a white-tailed deer in the United States.
abstract: In November 2004, tissues were collected from a hunter-killed white-tailed deer in St. Mary Parish, Louisiana. Bluetongue virus (BTV) was isolated from the tissues; however, the isolate could not be identified as any of the US domestic serotypes. Subsequent testing by virus neutralization using serotype-specific antiserum tentatively identified the isolate as BTV serotype 1 (BTV-1), which had not previously been found in the United States. Primers were designed based on the sequence of an outer capsid protein gene of a South African BTV-1 strain. Reverse transcription-polymerase chain reaction testing with the BTV-1 primers and product sequencing confirmed the Louisiana isolate as BTV-1. This is the first report of BTV-1 in the United States.
authors.affiliation: Diagnostic Virology Laboratory, National Veterinary Services Laboratories, Ames, IA 50010, USA.
authors.forename: Donna J
authors.initials: DJ
authors.lastname: Johnson
authors.forename: Eileen N
authors.initials: EN
authors.lastname: Ostlund
authors.forename: David E
authors.initials: DE
authors.lastname: Stallknecht
authors.forename: Virginia H
authors.initials: VH
authors.lastname: Goekjian
authors.forename: Melinda
authors.initials: M
authors.lastname: Jenkins-Moore
authors.forename: Steven C
authors.initials: SC
authors.lastname: Harris
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000175:diagnosis / Q000821:virology*; D001820:Bluetongue virus / Q000145:classification* / Q000302:isolation & purification*; D003670:Deer / Q000821:virology*; D012756:Sheep; D014481:United States