Murine polyomavirus-like particles induce maturation of bone marrow-derived dendritic cells and proliferation of T cells.
We analysed the effects of murine polyomavirus-like particles (PLPs) on bone marrow-derived dendritic cells (BMDCs) and T cells in vitro. BMDCs activated with PLPs up-regulated CD40, CD80, CD86 and major histocompatibility complex (MHC) class II surface markers and produced proinflammatory cytokines. Chimeric PLPs [expressing the ovalbumin (OVA)-peptides OVA(257-264) or OVA(323-339)], but not wildtype PLPs, activated OVA-specific CD8 T cells and OVA-specific CD4 T cells, respectively, indicating both MHC class I and II presentation of the peptides by antigen-presenting cells. Our results suggest that PLPs may be used as vaccine adjuvants priming dendritic cells to induce potent T cell responses.
Bickert
Thomas
T
Center for Infectious Diseases and Institute for Molecular Infection Biology, University of Wuerzburg, Wuerzburg, Germany. bickert@bni-hamburg.de
Wohlleben
Gisela
G
Brinkman
Marc
M
Trujillo-Vargas
Claudia M
CM
Ruehland
Claus
C
Reiser
Christian O A
CO
Hess
Juergen
J
Erb
Klaus J
KJ
D000276:Adjuvants, Immunologic; D000818:Animals; D017951:Antigen Presentation; D015703:Antigens, CD / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin; D011120:Polyomavirus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000276:Adjuvants, Immunologic; D015703:Antigens, CD; D016207:Cytokines; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D010047:Ovalbumin