title: Effect of inhibitors released during steam-explosion pretreatment of barley straw on enzymatic hydrolysis.
abstract: The influence of the liquid fraction (prehydrolysate) generated during steam-explosion pretreatment (210 degrees C, 15 min) of barley straw on the enzymatic hydrolysis was determined. Prehydrolysate was analyzed for degradation compounds and sugars' content and used as a medium for enzymatic hydrolysis tests after pH adjusting to 4.8. Our results show that the presence of the compounds contained in the prehydrolysate strongly affects the hydrolysis step (a 25% decrease in cellulose conversion compared with control). Sugars are shown to be more potent inhibitors of enzymatic hydrolysis than degradation products.
authors.affiliation: Renewable Energies Department-CIEMAT, Avenida Complutense, 22 28040-Madrid, Spain.
authors.forename: Ma Prado
authors.initials: MP
authors.lastname: García-Aparicio
authors.forename: Ignacio
authors.initials: I
authors.lastname: Ballesteros
authors.forename: Alberto
authors.initials: A
authors.lastname: González
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Oliva
authors.forename: Mercedes
authors.initials: M
authors.lastname: Ballesteros
authors.forename: Ma José
authors.initials: MJ
authors.lastname: Negro
mesh_terms: D002480:Cellulase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000138:chemical synthesis; D001467:Hordeum / Q000737:chemistry*; D006358:Hot Temperature; D006868:Hydrolysis; D035261:Plant Components, Aerial / Q000737:chemistry*; D013227:Steam
chemical_list: D004791:Enzyme Inhibitors; D013227:Steam; D002482:Cellulose; D002480:Cellulase