title: Characterization of alpha-mannosidase from Erythrina indica seeds and influence of endogenous lectin on its activity.
abstract: alpha-mannosidase from Erythrina indica seeds is a Zn(2+) dependent glycoprotein with 8.6% carbohydrate. The enzyme has a temperature optimum of 50 degrees C and energy of activation calculated from Arrhenius plot was found to be 23 kJ mol(-1). N-terminal sequence up to five amino acid residues was found to be DTQEN (Asp, Thr, Gln, Glu, and Asn). In chemical modification studies treatment of the enzyme with NBS led to total loss of enzyme activity and modification of a single tryptophan residue led to inactivation. Fluorescence studies over a pH range of 3-8 have shown tryptophan residue to be in highly hydrophobic environment and pH change did not bring about any appreciable change in its environment. Far-UV CD spectrum indicated predominance of alpha-helical structure in the enzyme. alpha-Mannosidase from E indica exhibits immunological identity with alpha-mannosidase from Canavalia ensiformis but not with the same enzyme from Glycine max and Cicer arietinum. Incubation of E. indica seed lectin with alpha-mannosidase resulted in 35% increase in its activity, while no such activation was observed for acid phosphatase from E. indica. Lectin induced activation of alpha-mannosidase could be completely abolished in presence of lactose, a sugar specific for lectin.
authors.affiliation: Division of Biochemistry, Department of Chemistry, University of Pune, Pune, India.
authors.forename: Rakesh Mohan
authors.initials: RM
authors.lastname: Kestwal
authors.forename: Emadeldin Hassan E
authors.initials: EH
authors.lastname: Konozy
authors.forename: Chwan-Deng
authors.initials: CD
authors.lastname: Hsiao
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Roque-Barreira
authors.forename: Shobhana V
authors.initials: SV
authors.lastname: Bhide
mesh_terms: D002942:Circular Dichroism; D004895:Erythrina / Q000196:embryology / Q000201:enzymology*; D037102:Lectins / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology*; D013050:Spectrometry, Fluorescence; D043323:alpha-Mannosidase / Q000378:metabolism*
chemical_list: D037102:Lectins; D043323:alpha-Mannosidase