title: Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureus.
abstract: The aim of this study was to evaluate the antimicrobial potential of ozone applied to 3 different solutions in an ultrasonic cleaning system against Staphylococcus aureus. A total of 120 mL of S. aureus were mixed in 6 L of the experimental solutions (sterile distilled water, vinegar and sterile distilled water + Endozime AWpluz) used in a ultrasonic cleaning system (UCS). Ozone was produced by an electric discharge through a current of oxygen and bubbling with flow rate at 7 g/h ozone (1.2%) into the microbial suspensions. Ten mL of each experimental suspension were collected and 5 fold dilutions were made in 9 mL of BHI and incubated at 37 degrees C for 48 h. Bacterial growth was evaluated by turbidity of the culture medium. At the same time, 1 mL of bacterial samples was collected and inoculated in BHIA plates. After incubation at 37 degrees C for 48 h, the number of colony forming units (cfu) per mL on BHIA surface was counted. In dilution test in BHI tubes and in BHIA plates (cfu/mL), bacterial growth was not observed in any of the experimental solutions when ozone was added. Under the tested conditions, it may be concluded that the addition of ozone to a ultrasonic cleaning system containing different experimental solutions resulted in antibacterial activity against S. aureus.
authors.affiliation: Faculty of Dentistry, Federal University of Goiás, Goiânia, GO, Brazil. estrela3@terra.com.br
authors.forename: Carlos
authors.initials: C
authors.lastname: Estrela
authors.forename: Cyntia R A
authors.initials: CR
authors.lastname: Estrela
authors.forename: Daniel de Almeida
authors.initials: Dde A
authors.lastname: Decurcio
authors.forename: Julio Almeida
authors.initials: JA
authors.lastname: Silva
authors.forename: Lili Luschke
authors.initials: LL
authors.lastname: Bammann
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D006801:Humans; D019552:Infection Control, Dental / Q000295:instrumentation / Q000379:methods*; D010126:Ozone / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects*; D014465:Ultrasonics
chemical_list: D000890:Anti-Infective Agents; D010126:Ozone