Quantitative determination of eight components in rhizome (Jianghuang) and tuberous root (Yujin) of Curcuma longa using pressurized liquid extraction and gas chromatography-mass spectrometry.
Curcuma longa (Zingiberaceae) is a native plant of southern Asia and is cultivated extensively throughout the warmer parts of the world. Jianghuang and Yujin are rhizome and tuberous root of C. longa, respectively, which were traditionally used as two Chinese medicines. In this paper, pressurized liquid extraction (PLE) and gas chromatography-mass spectrometry (GC-MS) were developed for quantitative determination/estimation of eight characteristic compounds including beta-caryophyllene, ar-curcumene, zingiberene, beta-bisabolene, beta-sesquiphellandrenendrene, ar-turmerone, alpha-turmerone and beta-turmerone in Jianghuang and Yujin. A HP-5MS capillary column (30 m x 0.25 mm i.d.) coated with 0.25 microm film 5% phenyl methyl siloxane was used for separation and selected ion monitoring (SIM) method was used for quantitation. Hierarchical cluster analysis based on characteristics of eight identified peaks in GC-MS profiles showed that 10 samples were divided into two main clusters, Jianghuang and Yujin, respectively. Four components such as ar-curcumene, ar-turmerone, alpha-turmerone and beta-turmerone were optimized as markers for quality control of rhizome (Jianghuang) and tuberous root (Yujin), which are two traditional Chinese medicines, from Curcuma longa.
Qin
N Y
NY
Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, China.
Yang
F Q
FQ
Wang
Y T
YT
Li
S P
SP
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000031:analogs & derivatives / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007659:Ketones / Q000032:analysis; D015394:Molecular Structure; D000081222:Monocyclic Sesquiterpenes; D035281:Plant Tubers; D000081223:Polycyclic Sesquiterpenes; D011786:Quality Control; D027343:Rhizome; D012717:Sesquiterpenes / Q000032:analysis; D013678:Technology, Pharmaceutical / Q000379:methods*; D014050:Toluene / Q000031:analogs & derivatives / Q000032:analysis
D004365:Drugs, Chinese Herbal; D007659:Ketones; D000081222:Monocyclic Sesquiterpenes; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C078098:ar-turmerone; D014050:Toluene; C086829:alpha-curcumene; C451028:beta-turmerone; C477983:zingiberene; C024714:caryophyllene; D003474:Curcumin