Genetic introduction of a diketone-containing amino acid into proteins.
An orthogonal tRNA/aminoacyl-tRNA synthetase pair was evolved that makes possible the site-specific incorporation of an unnatural amino acid bearing a beta-diketone side chain into proteins in Escherichia coli with high translational efficiency and fidelity. Proteins containing this unnatural amino acid can be efficiently and selectively modified with hydroxylamine derivatives of fluorophores and other biophysical probes.
Zeng
Huaqiang
H
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Xie
Jianming
J
Schultz
Peter G
PG
D000596:Amino Acids / Q000737:chemistry*; D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry; D001665:Binding Sites; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007659:Ketones / Q000737:chemistry*; D008968:Molecular Conformation; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D015202:Protein Engineering / Q000379:methods; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D012343:RNA, Transfer / Q000737:chemistry; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D000596:Amino Acids; D007659:Ketones; D011506:Proteins; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases