title: Respiratory response to endotoxin and dust predicts evidence of inflammatory response in volunteers in a swine barn.
abstract: BACKGROUND
  It has been shown that exposure in intense exposure in swine barn facilities is associated with increased respiratory symptoms and reduction in pulmonary functions. This study investigated if systemic response could be predicted by FEV(1) response following swine barn exposure.
  
  
  METHODS
  Naïve males were tested at baseline, low and high endotoxin and dust levels. Subjects were classified as "more responsive" (n = 9) or "less responsive" (n = 11) based on FEV(1) reduction following high endotoxin exposure. Health measures included pulmonary function testing, blood samples and nasal lavage. Environmental samples were collected from the barn.
  
  
  RESULTS
  White blood cells and blood lymphocytes at low exposure were significantly greater in those who were "more responsive" compared to those who were "less responsive". There was a significant increase in blood lymphocytes, serum IL6, total nasal lavage cells and nasal IL8 at high exposure among "more responsive" subjects compared to "less responsive" subjects.
  
  
  CONCLUSIONS
  Respiratory response to high-level endotoxin and dust exposure predicts evidence of inflammatory response throughout a range of endotoxin and dust exposures.
authors.affiliation: Institute of Agricultural Rural and Environmental Health, IAREH, University of Saskatchewan, Saskatoon, Canada. dosman@sask.usask.ca
authors.forename: J A
authors.initials: JA
authors.lastname: Dosman
authors.forename: Y
authors.initials: Y
authors.lastname: Fukushima
authors.forename: A
authors.initials: A
authors.lastname: Senthilselvan
authors.forename: S P
authors.initials: SP
authors.lastname: Kirychuk
authors.forename: J A
authors.initials: JA
authors.lastname: Lawson
authors.forename: P
authors.initials: P
authors.lastname: Pahwa
authors.forename: Y
authors.initials: Y
authors.lastname: Cormier
authors.forename: T
authors.initials: T
authors.lastname: Hurst
authors.forename: E M
authors.initials: EM
authors.lastname: Barber
authors.forename: C S
authors.initials: CS
authors.lastname: Rhodes
mesh_terms: D000293:Adolescent; D000328:Adult; D000395:Air Pollutants, Occupational / Q000009:adverse effects* / Q000032:analysis; D000822:Animal Husbandry; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D018592:Cross-Over Studies; D004391:Dust / Q000032:analysis*; D004731:Endotoxins / Q000009:adverse effects* / Q000032:analysis; D006799:Housing, Animal; D006801:Humans; D006967:Hypersensitivity / Q000453:epidemiology* / Q000276:immunology; D015850:Interleukin-6 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D008297:Male; D016273:Occupational Exposure / Q000009:adverse effects* / Q000032:analysis; D012129:Respiratory Function Tests; D012140:Respiratory Tract Diseases / Q000453:epidemiology* / Q000276:immunology; D018709:Statistics, Nonparametric; D013552:Swine
chemical_list: D000395:Air Pollutants, Occupational; D015415:Biomarkers; D004391:Dust; D004731:Endotoxins; D015850:Interleukin-6; D016209:Interleukin-8