Cellobiase from Termitomyces clypeatus: activity and secretion in presence of glycosylation inhibitors.
In presence of the glycosylation inhibitors, 2-deoxy-D-glucose (1 mg/ml), tunicamycin (30 microg/ml), 1-deoxynojirimycin (30 microg/ml) and D-glucono-delta-lactone (1 mg/ml), total cellobiase activity, in the extracellular, intracellular and cell bound fractions, of the fungus Termitomyces clypeatus grown in 20 ml cellobiose medium (1%, w/v) increased by 50-, 1.8-, 2.4-, 1.3-fold, respectively, with respect to control medium (16.3 U). The inhibitors also stimulated secretion of 95% of the total protein in culture medium, except D-glucono-delta-lactone which released 60% of the total protein. 2-Deoxy-D-glucose (1 mg/ml) led to production of extracellular cellobiase up to 40 U/ml, whereas in absence of the inhibitors only 0.59 U/ml enzyme was detected.
Mukherjee
Sumana
S
Department of Applied Biochemistry, Indian Institute of Chemical Biology, 4 Raja S C Mullick Road, Kolkata 700032, India.
Chowdhury
Sudeshna
S
Ghorai
Shakuntala
S
Pal
Swagata
S
Khowala
Suman
S
D017485:1-Deoxynojirimycin / Q000494:pharmacology; D000363:Agaricales / Q000201:enzymology* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002475:Cellobiose / Q000378:metabolism*; D003847:Deoxyglucose / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005942:Gluconates / Q000494:pharmacology; D006031:Glycosylation / Q000187:drug effects; D007783:Lactones; D014415:Tunicamycin / Q000494:pharmacology; D001617:beta-Glucosidase / Q000737:chemistry / Q000378:metabolism*
D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D005942:Gluconates; D007783:Lactones; D014415:Tunicamycin; D002475:Cellobiose; D017485:1-Deoxynojirimycin; D003847:Deoxyglucose; D001617:beta-Glucosidase; C010730:beta-glucono-1,5-lactone