title: [Co-culture of cryopreserved rat islets with small intestinal submucosa].
abstract: OBJECTIVE
  To investigate the feasibility and benefits of co-culture of cryopreserved islets with small intestinal submucosa (SIS).
  
  
  METHODS
  Purified rat islets cryopreserved for one month were divided into SIS group and control group, and after culture in standard islet culture media RPMI1640 for 1 week, the morphology and function of the islets were assessed.
  
  
  RESULTS
  The SIS protects the fragile islets from damage by cryopreservation, and increased the recovery from (60.6-/+3.3)% to (91.7-/+1.8) % (P<0.05). Compared with the control group, incubation of the islets of the SIS group in high-glucose (16.7 mmol/L) solution resulted in significantly enhanced insulin secretion (23.7-/+1.6 vs 12.5-/+1.1 mU/L, P<0.05). When the islets were incubated in high-glucose solution containing theophylline, the calculated stimulation index of SIS group was about 3-fold higher than that of the control group.
  
  
  CONCLUSIONS
  Co-culture of cryopreserved rat islets with SIS can increase the recovery of islet cells and improve their function.
authors.affiliation: Center of Renal Transplantation, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China. houjundoctor@sina.com
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: He-li
authors.initials: HL
authors.lastname: Xiang
authors.forename: Xin-shun
authors.initials: XS
authors.lastname: Feng
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Tian
authors.forename: Xin-lu
authors.initials: XL
authors.lastname: Pang
authors.forename: Yan
authors.initials: Y
authors.lastname: Teng
mesh_terms: D000818:Animals; D018920:Coculture Techniques; D015925:Cryopreservation / Q000379:methods*; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D007421:Intestine, Small / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013806:Theophylline / Q000494:pharmacology
chemical_list: D007328:Insulin; D013806:Theophylline; D005947:Glucose