title: Associations between two single nucleotide polymorphisms of adiponectin gene and coronary artery diseases.
abstract: Adiponectin, an adipocyte-secreted protein, is known to have anti-atherogenic, anti-inflammatory and anti-diabetic properties and its serum levels are decreased in obesity, type 2 diabetes, and coronary artery disease. Several studies have been performed to investigate the association of genetic variations in the adiponectin with obesity, insulin resistance, and type 2 diabetes, but few studies were performed in association with coronary artery disease. Therefore we examined the associations between two single nucleotide polymorphisms (SNPs), +45T>G and +276G>T of the adiponectin gene, and coronary artery diseases (CAD). One hundred and fifty six subjects (mean age 57.4 yrs) were enrolled in which coronary angiograms were performed due to chest pain. Genotypings were done for two SNPs in the adiponectin gene by Taqman polymerase chain reaction (PCR) method. The presence of CAD was defined as a >50% reduction of coronary artery diameter. Among 156 subjects, the allele frequencies were 0.683 for G allele and 0.317 for T allele in SNP +276G>T and 0.705 for T allele and 0.295 for G allele in SNP +45T>G. Both genotypes were in compliance with Hardy-Weinberg equilibrium. No association with the presence of CAD was observed for adiponectin gene SNP276 and SNP45 (p = 0.954, p = 0.843). Also, no significant association was observed between the severity of CAD and either SNPs (p = 0.571, p = 0.955). Our study showed that SNP +276G>T and +45T>G in adiponectin gene were not associated with the presence of CAD. Further studies will be necessary to confirm the role of SNP 276G>T and 45T>G in the development of CAD.
authors.affiliation: Department of Internal Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, South Korea.
authors.forename: Chan-Hee
authors.initials: CH
authors.lastname: Jung
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Rhee
authors.forename: Se-Yeon
authors.initials: SY
authors.lastname: Kim
authors.forename: Hun-Sub
authors.initials: HS
authors.lastname: Shin
authors.forename: Byung-Jin
authors.initials: BJ
authors.lastname: Kim
authors.forename: Ki-Chul
authors.initials: KC
authors.lastname: Sung
authors.forename: Bum-Su
authors.initials: BS
authors.lastname: Kim
authors.forename: Won-Young
authors.initials: WY
authors.lastname: Lee
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Kang
authors.forename: Ki-Won
authors.initials: KW
authors.lastname: Oh
authors.forename: Man-Ho
authors.initials: MH
authors.lastname: Lee
authors.forename: Sun-Woo
authors.initials: SW
authors.lastname: Kim
authors.forename: Jung-Roe
authors.initials: JR
authors.lastname: Park
mesh_terms: D052242:Adiponectin / Q000235:genetics*; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D052242:Adiponectin