Effect of inhibitors released during steam-explosion pretreatment of barley straw on enzymatic hydrolysis.
The influence of the liquid fraction (prehydrolysate) generated during steam-explosion pretreatment (210 degrees C, 15 min) of barley straw on the enzymatic hydrolysis was determined. Prehydrolysate was analyzed for degradation compounds and sugars' content and used as a medium for enzymatic hydrolysis tests after pH adjusting to 4.8. Our results show that the presence of the compounds contained in the prehydrolysate strongly affects the hydrolysis step (a 25% decrease in cellulose conversion compared with control). Sugars are shown to be more potent inhibitors of enzymatic hydrolysis than degradation products.
García-Aparicio
Ma Prado
MP
Renewable Energies Department-CIEMAT, Avenida Complutense, 22 28040-Madrid, Spain.
Ballesteros
Ignacio
I
González
Alberto
A
Oliva
José Miguel
JM
Ballesteros
Mercedes
M
Negro
Ma José
MJ
D002480:Cellulase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000138:chemical synthesis; D001467:Hordeum / Q000737:chemistry*; D006358:Hot Temperature; D006868:Hydrolysis; D035261:Plant Components, Aerial / Q000737:chemistry*; D013227:Steam
D004791:Enzyme Inhibitors; D013227:Steam; D002482:Cellulose; D002480:Cellulase