Liposome-mediated RNA transfection should be used with caution.
Liposome-mediated RNA transfection appears to present a number of advantages for studying the metabolism of reporter mRNAs in mammalian cells. This method is also widely used to transfect siRNAs. Here we describe results indicating that reporter mRNAs introduced into HeLa cells by liposomes do not present the expected behaviors. Namely, the stability of reporter mRNAs was independent of the presence or absence of an AUUUA instability element, a poly(A) tail, or even a 5' methylated cap. Confocal microscopy showed that fluorescent RNAs introduced by liposome-mediated transfection were present in discrete particles. These observations imply that a number of control experiments are required when using liposome to mediated RNA transfection, and the possible consequences are discussed.
Barreau
Carine
C
Dutertre
Stéphanie
S
Paillard
Luc
L
Osborne
H Beverley
HB
D005456:Fluorescent Dyes; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes; D018613:Microscopy, Confocal; D012313:RNA / Q000008:administration & dosage* / Q000235:genetics* / Q000378:metabolism; D020871:RNA Stability; D012333:RNA, Messenger / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D014162:Transfection / Q000379:methods*
D005456:Fluorescent Dyes; D008081:Liposomes; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012313:RNA