Cross-species hybridization of woodchuck hepatitis virus-induced hepatocellular carcinoma using human oligonucleotide microarrays.
OBJECTIVE
To demonstrate the feasibility of using woodchuck samples on human microarrays, to provide insight into pathways involving positron emission tomography (PET) imaging tracers and to identify genes that could be potential molecular imaging targets for woodchuck hepatocellular carcinoma.


METHODS
Labeled cRNA from woodchuck tissue samples were hybridized to Affymetrix U133 plus 2.0 GeneChips. Ten genes were selected for validation using quantitative RT-PCR and literature review was made.


RESULTS
Testis enhanced gene transcript (BAX Inhibitor 1), alpha-fetoprotein, isocitrate dehydrogenase 3 (NAD+) beta, acetyl-CoA synthetase 2, carnitine palmitoyltransferase 2, and N-myc2 were up-regulated and spermidine/spermine N1-acetyltransferase was down-regulated in the woodchuck HCC. We also found previously published results supporting 8 of the 10 most up-regulated genes and all 10 of the 10 most down-regulated genes.


CONCLUSIONS
Many of our microarray results were validated using RT-PCR or literature search. Hence, we believe that woodchuck HCC and non-cancerous liver samples can be used on human microarrays to yield meaningful results.
Anderson
Paul W
PW
Department of Radiology, University Hospitals of Cleveland, Cleveland, OH 44106, USA.
Tennant
Bud C
BC
Lee
Zhenghong
Z
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000821:virology; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D004195:Disease Models, Animal; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D017919:Hepatitis B Virus, Woodchuck / Q000235:genetics*; D006801:Humans; D006824:Hybridization, Genetic / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics* / Q000821:virology; D008392:Marmota; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011786:Quality Control; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004273:DNA, Neoplasm; D004247:DNA