title: [Molecular taxonomy techniques used for yeast identification].
abstract: Due to the major impact of yeasts in human life based on the existence of pathogen yeast species and of species with biotechnological abilities, in the last few years new molecular techniques are performed for an accurate identification of natural isolates. Our study is aimed to review some of these techniques such as electrokariotyping by PFGE (Pulsed Field Gel Electrophoresis), estimation of the molar percentage of guanine and cytosine, the applications of PCR reaction in yeast identification using RAPD (Random amplified polymorphic DNA), UP-PCR (Universally Primed Polymerase Chain Reaction), MLST (Multilocus sequence typing) techniques, mtDNA and rDNA homology studies. Such molecular techniques complete the phenotypical characterization based on classical taxonomical tests allowing thus the polyphasic identification of the microorganisms.
authors.affiliation: Universitatea Bucureşti, Facultatea de Biologie, Centrul de Cercetare, Formare şi Consultanţă in Microbiologie, Genetică şi Biotehnologie.
authors.forename: Raluca
authors.initials: R
authors.lastname: Ghindea
authors.forename: Ortansa
authors.initials: O
authors.lastname: Csutak
authors.forename: Ileana
authors.initials: I
authors.lastname: Stoica
authors.forename: Robertina
authors.initials: R
authors.lastname: Ionescu
authors.forename: Simona
authors.initials: S
authors.lastname: Soare
authors.forename: Diana
authors.initials: D
authors.lastname: Pelinescu
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Nohit
authors.forename: Oana
authors.initials: O
authors.lastname: Creangă
authors.forename: Tatiana
authors.initials: T
authors.lastname: Vassu
mesh_terms: D003596:Cytosine; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D016521:Electrophoresis, Gel, Pulsed-Field; D006147:Guanine; D006801:Humans; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D006147:Guanine; D003596:Cytosine