Genetic characterization of Trypanosoma evansi isolated from a patient in India.
The first human case of trypanosomiasis caused by Trypanosoma evansi was recently discovered in India. We have focused on the parasite to investigate whether this atypical infection was due to a particular genotype of T. evansi. The SRA gene was not detected by PCR in the Indian human T. evansi (TEVH) DNA sample. TEVH appears to be closely related to Vietnam WH, with identical alleles for TRBPA and MT30-33 AC/TC microsatellites. Furthermore, T. evansi has homogeneous kDNA minicircles and the minicircles of isolate TEVH were shown to be of Type A. Thus, the T. evansi isolated from an Indian patient appears to be a typical T. evansi as far as we can judge, suggesting that the explanation for this unusual infection may lie with the patient.
Truc
Philippe
P
Institut de Recherche pour le Développement, UR177, Instituto de Combate e Controlo das Tripanossomiases (ICCT), CP 2657, Luanda, Angola. truc@ird.fr
Gibson
Wendy
W
Herder
Stephane
S
D000818:Animals; D018105:DNA, Kinetoplast / Q000235:genetics; D017125:Genes, Protozoan; D014644:Genetic Variation; D006801:Humans; D007194:India; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D014345:Trypanosoma / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014352:Trypanosomiasis / Q000276:immunology / Q000469:parasitology*
D018105:DNA, Kinetoplast