title: Mitochondrial heat shock protein 60 is increased in cerebrospinal fluid following pediatric traumatic brain injury.
abstract: Mitochondrial dysfunction occurs after traumatic brain injury (TBI) and contributes significantly to subsequent cell death. Heat shock protein 60 (hsp60) is a predominantly mitochondrial protein with important homeostatic functions. Induction of hsp60 has been demonstrated in cerebral ischemia models, possibly reflecting mitochondrial stress. We measured hsp60 concentration in the cerebrospinal fluid (CSF) of 34 infants and children after severe TBI and of 7 control patients by ELISA. Peak CSF hsp60 concentration was increased in TBI patients versus controls (0.84 ng/ml, range 0-44.59, vs. 0.0 ng/ml, range 0-0.48; p<0.05). Induction of hsp60 occurred early after the injury. Peak hsp60 concentration was independently associated with the severity of injury, defined as the admission Glasgow Coma Scale score. These data suggest that increased hsp60 in CSF might reflect the severity of early mitochondrial stress or damage after TBI.
authors.affiliation: Department of Critical Care Medicine, Safar Center for Resuscitation Research, University of Pittsburgh School of Medicine, and Children's Hospital of Pittsburgh, PA 15213, USA.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Lai
authors.forename: Christopher
authors.initials: C
authors.lastname: Stange
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Wisniewski
authors.forename: P David
authors.initials: PD
authors.lastname: Adelson
authors.forename: Keri L
authors.initials: KL
authors.lastname: Janesko-Feldman
authors.forename: Danielle S
authors.initials: DS
authors.lastname: Brown
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Kochanek
authors.forename: Robert S B
authors.initials: RS
authors.lastname: Clark
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000134:cerebrospinal fluid; D001921:Brain / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D001930:Brain Injuries / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000503:physiopathology; D018834:Chaperonin 60 / Q000032:analysis / Q000134:cerebrospinal fluid*; D002648:Child; D002675:Child, Preschool; D004734:Energy Metabolism / Q000502:physiology; D005260:Female; D015600:Glasgow Coma Scale; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D028361:Mitochondrial Diseases / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000503:physiopathology; D011237:Predictive Value of Tests; D011379:Prognosis; D013312:Stress, Physiological / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D015854:Up-Regulation / Q000502:physiology
chemical_list: D015415:Biomarkers; D018834:Chaperonin 60