Open microscopy environment and findspots: integrating image informatics with quantitative multidimensional image analysis.
Biomedical research and drug development increasingly involve the extraction of quantitative data from digital microscope images, such as those obtained using fluorescence microscopy. Here, we describe a novel approach for both managing and analyzing such images. The Open Microscopy Environment (OME) is a sophisticated open-source scientific image management database that coordinates the organization, storage, and analysis of the large volumes of image data typically generated by modern imaging methods. We describe FindSpots, a powerful image-analysis package integrated in OME that will be of use to those who wish to identify and measure objects within microscope images or time-lapse movies. The algorithm used in FindSpots is in fact only one of many possible segmentation (object detection) algorithms, and the underlying data model used by OME to capture and store its results can also be used to store results from other segmentation algorithms. In this report, we illustrate how image segmentation can be achieved in OME using one such implementation of a segmentation algorithm, and how this output subsequently can be displayed graphically or processed numerically using a spreadsheet.
Schiffmann
David A
DA
University of Dundee, Dundee, Scotland, UK.
Dikovskaya
Dina
D
Appleton
Paul L
PL
Newton
Ian P
IP
Creager
Douglas A
DA
Allan
Chris
C
Näthke
Inke S
IS
Goldberg
Ilya G
IG
D000465:Algorithms; D000818:Animals; D019295:Computational Biology; D003628:Database Management Systems; D016208:Databases, Factual; D005347:Fibroblasts / Q000378:metabolism; D005456:Fluorescent Dyes; D007090:Image Interpretation, Computer-Assisted; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D007211:Indoles; D016247:Information Storage and Retrieval; D018386:Kinetochores / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D011494:Protein Kinases / Q000032:analysis; D017346:Protein Serine-Threonine Kinases; D015203:Reproducibility of Results; D014584:User-Computer Interface
D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; D011494:Protein Kinases; C090464:Bub1 spindle checkpoint protein; D017346:Protein Serine-Threonine Kinases