"Chip"ping away at heart failure.
Studies in the field of microarray technology have exploded onto the scene to delve into the unknown underlying mechanisms and pathways in molecular disease. Diseases of the cardiovascular system, particularly those with unexplained molecular etiologies, such as heart failure, have more recently been investigated using array technology. Our laboratory has sought to examine gene expression profiles of human heart failure using a 10,000+ element cardiovascular-based complementary DNA microarray constructed in-house, termed the "CardioChip." Our studies have identified panels of genes, such as those encoding sarcomeric and cytoskeletal proteins, stress proteins, and Ca2+ regulators, that are differentially expressed in disease conditions as compared with samples from nonfailing hearts. Microarrays are effective tools for examining molecular portraits of the cardiovascular disease condition.
Barrans
J David
JD
Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.
Liew
Choong-Chin
CC
D000328:Adult; D020224:Expressed Sequence Tags; D005333:Fetus / Q000378:metabolism; D005456:Fluorescent Dyes; D020869:Gene Expression Profiling; D006333:Heart Failure / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005456:Fluorescent Dyes; D012333:RNA, Messenger