title: A resistance-like gene identified by EST mapping and its association with a QTL controlling Fusarium head blight infection on wheat chromosome 3BS.
abstract: Fusarium head blight (FHB) is a major disease in the wheat growing regions of the world. A quantitative trait locus (QTL) on the short arm of chromosome 3B controls much of the variation for resistance. The cloning of candidate disease-resistance genes for FHB QTLs on chromosome 3B can provide further elucidation of the mechanisms that control resistance. However, rearrangements and divergence during plant genome evolution often hampers the identification of sequences with similarity to known disease-resistance genes. This study focuses on the use of wheat expressed sequence tags (ESTs) that map to the region on chromosome 3B containing the QTL for FHB resistance and low-stringency BLAST searching to identify sequences with similarity to known disease-resistance genes. One EST rich with leucine repeats and low similarity to a protein kinase domain of the barley Rpg1 gene was identified. Genetic mapping using a Ning894037 x Alondra recombinant inbred (RI) population showed that this EST mapped to the QTL on the short arm of chromosome 3B and may represent a portion of a newly diverged gene contributing to FHB resistance. The EST is a new marker suitable for marker-assisted selection and provides a starting point to begin map-based cloning for chromosome walking and investigate new diverged genes at this locus.
authors.affiliation: Department of Agronomy, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Shen
authors.forename: Michael G
authors.initials: MG
authors.lastname: Francki
authors.forename: Herbert W
authors.initials: HW
authors.lastname: Ohm
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D020224:Expressed Sequence Tags; D005670:Fusarium / Q000472:pathogenicity*; D017343:Genes, Plant; D007113:Immunity, Innate / Q000235:genetics; D008126:Lod Score; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics; D040641:Quantitative Trait Loci / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000235:genetics* / Q000382:microbiology