Peptide nucleic acid microarrays made with (S,S)-trans-cyclopentane-constrained peptide nucleic acids.
Procedures to attach trans-cyclopentane-modified peptide nucleic acid oligomers to a glass slide are described. Peptide nucleic acids can offer distinct advantages to DNA detection compared to typically used DNA microarrays, especially with regard to chemical stability and quality of data. The trans-cyclopentane modification incorporated into the peptide nucleic acid is, in some cases, essential for successful DNA detection. It is hoped that these peptide nucleic acid-bearing glass slides will find application in several areas of DNA microarray technology.
Witschi
Mark A
MA
Laboratory of Bioorganic Chemistry, NIDDK, NIH, DHHS, Bethesda, Maryland, USA.
Pokorski
Jonathan K
JK
Appella
Daniel H
DH
D003517:Cyclopentanes; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020135:Peptide Nucleic Acids
D003517:Cyclopentanes; D020135:Peptide Nucleic Acids