Transcriptional profiling reveals a possible role for the timing of the inflammatory response in determining susceptibility to a viral infection.
Using a novel cDNA microarray prepared from sources of actively responding immune system cells, we have investigated the changes in gene expression in the target tissue during the early stages of infection of neonatal chickens with infectious bursal disease virus. Infections of two lines of chickens previously documented as genetically resistant and sensitive to infection were compared in order to ascertain early differences in the response to infection that might provide clues to the mechanism of differential genetic resistance. In addition to major changes that could be explained by previously described changes in infected tissue, some differences in gene expression on infection, and differences between the two chicken lines, were observed that led to a model for resistance in which a more rapid inflammatory response and more-extensive p53-related induction of apoptosis in the target B cells might limit viral replication and consequent pathology. Ironically, the effect in the asymptomatic neonatal infection is that more-severe B-cell depletion is seen in the more genetically resistant chicken. Changes of expression of many chicken genes of unknown function, indicating possible roles in the response to infection, may aid in the functional annotation of these genes.
Ruby
Thomas
T
CNRS, UPR 1983, 7 rue Guy Moquet, 94800 Villejuif, France.
Whittaker
Catherine
C
Withers
David R
DR
Chelbi-Alix
Mounira K
MK
Morin
Veronique
V
Oudin
Anne
A
Young
John R
JR
Zoorob
Rima
R
D000818:Animals; D017209:Apoptosis; D002645:Chickens; D018076:DNA, Complementary / Q000378:metabolism; D016786:Eimeria tenella / Q000378:metabolism; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D007107:Immune System; D007249:Inflammation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014777:Virus Diseases / Q000209:etiology* / Q000235:genetics*
D018076:DNA, Complementary; D016159:Tumor Suppressor Protein p53