Environmental cues to guide stem cell fate decision for tissue engineering applications.
The human body contains a variety of stem cells capable of both repeated self-renewal and production of specialised, differentiated progeny. Critical to the implementation of these cells in tissue engineering strategies is a thorough understanding of which external signals in the stem cell microenvironment provide cues to control their fate decision in terms of proliferation or differentiation into a desired, specific phenotype. These signals must then be incorporated into tissue regeneration approaches for regulated exposure to stem cells. The precise spatial and temporal presentation of factors directing stem cell behaviour is extremely important during embryogenesis, development and natural healing events, and it is possible that this level of control will be vital to the success of many regenerative therapies. This review covers existing tissue engineering approaches to guide the differentiation of three disparate stem cell populations: mesenchymal, neural and endothelial. These progenitor cells will be of central importance in many future connective, neural and vascular tissue regeneration technologies.
Alsberg
Eben
E
Case Western Reserve University, Department of Biomedical Engineering, 10900 Euclid Avenue, Wickenden Building, Room 204, Cleveland, OH 44106-7207, USA. eben.alsberg@case.edu
von Recum
Horst A
HA
Mahoney
Melissa J
MJ
D000818:Animals; D002450:Cell Communication; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D016207:Cytokines / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000637:transplantation; D015316:Genetic Therapy / Q000639:trends; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000637:transplantation; D044968:Regenerative Medicine / Q000639:trends; D023822:Tissue Engineering / Q000639:trends*
D016207:Cytokines; D006133:Growth Substances