The high prevalence of the poor and ultrarapid metabolite alleles of CYP2D6, CYP2C9, CYP2C19, CYP3A4, and CYP3A5 in Taiwanese population.
Genetic polymorphisms of drug metabolizing enzymes, such as cytochromes P450 (CYPs), play major roles in the variations of drug responsiveness in human. The aim of this study is to identify the high prevalence (minor allele frequencies >1%) of the abnormal metabolite alleles of CYP2C9, CYP2C19, CYP2D6, CYP3A4, and CYP3A5 in the Taiwanese population. The genotyping of the functional single nucleotide polymorphisms (SNPs) of CYPs were conducted by direct exon sequencing in 180 Taiwanese volunteers. Twenty-one unique SNPs including three newly identified SNPs were detected in the Taiwanese population. Six of the 21 SNPs in five genes showed frequencies more than 1%. The results indicated that it could be very useful and important in developing an inexpensive, convenient, and precise genotyping method for the high prevalence of CYPs metabolizing abnormal alleles in the Taiwanese population.
Liou
Ya-Huei
YH
Research and Product Development, Vita Genomics Inc., 7Fl., No.6, Sec.1, Jungshing Road, Wugu Shiang, Taipei County, 248, Taiwan.
Lin
Chien-Ting
CT
Research and Product Development, Vita Genomics Inc., 7Fl., No.6, Sec.1, Jungshing Road, Wugu Shiang, Taipei County, 248, Taiwan.
Wu
Ying-Jye
YJ
Research and Product Development, Vita Genomics Inc., 7Fl., No.6, Sec.1, Jungshing Road, Wugu Shiang, Taipei County, 248, Taiwan.
Wu
Lawrence Shih-Hsin
LS
Research and Product Development, Vita Genomics Inc., 7Fl., No.6, Sec.1, Jungshing Road, Wugu Shiang, Taipei County, 248, Taiwan. lawrence.wu@vitagenomics.com.
D000483:Alleles; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics; D044466:Asian People / Q000235:genetics; D065731:Cytochrome P-450 CYP2C19; D065729:Cytochrome P-450 CYP2C9; D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006899:Mixed Function Oxygenases / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D013624:Taiwan
D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C450260:CYP2C9 protein, human; D065729:Cytochrome P-450 CYP2C9; D001189:Aryl Hydrocarbon Hydroxylases; C045793:CYP2C19 protein, human; C510164:CYP3A5 protein, human; D065731:Cytochrome P-450 CYP2C19; D019389:Cytochrome P-450 CYP2D6; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human