Blood T-cell repertoire in idiopathic nephrotic syndrome recurrence following kidney transplantation.
Corticosteroid resistant idiopathic nephrotic syndrome (CR-INS) is a glomerulopathy that recurs after kidney transplantation in 30-50% of patients, suggesting the involvement of systemic albuminuric factors, probably produced by activated T cells. We investigated peripheral T-cell selection and expansion before and after transplantation to identify and characterize T-lymphocyte patterns potentially associated with INS recurrence. We used a combined qualitative and quantitative assessment of Vbeta mRNA alterations at the level of the complementary determining region 3-length distribution (CDR3-LD) of the T-cell receptor (TCR). Peripheral blood mononuclear cells (PBMC) were collected from 18 CR-INS patients (8 with recurrence and 10 without recurrence) on the day of transplantation as well as at 1 month, 1 year and 5 years after transplantation, and Vbeta transcriptomes were analyzed. Our data show that blood T cells from patients with INS recurrence display a TCR repertoire that is stable in time and has a similar level of CDR3-LD alterations as the T-cell repertoire of control patients, both before and after transplantation. These results suggest that the process of INS recurrence does not involve TCR activation or specific clonal expansion of T cells. However, these results do not exclude a role for T cells in the production of an albuminuric factor.
Hervé
C
C
Institut National de la Santé Et de la Recherche Médicale and Nantes University, Unité Mixte 643: Immunointervention dans les Allo et Xénotransplantations, Institut de Transplantation et de Recherche en Transplantation CHU Hôtel Dieu, France.
Le Berre
L
L
Miqueu
P
P
Degauque
N
N
Ruiz
C
C
Brouard
S
S
Guillet
M
M
Soulillou
J P
JP
Dantal
J
J
D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects* / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009404:Nephrotic Syndrome / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D012008:Recurrence; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell