title: The contribution of PspC to pneumococcal virulence varies between strains and is accomplished by both complement evasion and complement-independent mechanisms.
abstract: Pneumococcal surface protein C (PspC) is a virulence factor of Streptococcus pneumoniae previously shown to play a role in bacterial adherence, invasion, and evasion of complement. We investigated the role of this protein in our murine models of pneumococcal pneumonia with different pneumococcal strains. The deletion of pspC in strains of serotypes 2, 3, and 19F did not significantly alter host survival times in the pneumonia model. In contrast, pspC deletion significantly reduced the virulence of the serotype 4 strain, TIGR4, in both the pneumonia and bacteremia models. Therefore, pspC is a systemic and pulmonary virulence determinant for S. pneumoniae, but its effects are influenced by the pneumococcal strain. Finally, pneumonia infection of complement-deficient (C3(-/-)) mice enhanced pspC virulence, illustrating that PspC-mediated complement evasion contributes to virulence. However, other functions of PspC also contribute to virulence, as demonstrated by the finding that the pspC-deficient TIGR4 mutant was still attenuated relative to the wild-type parent, even in the absence of C3.
authors.affiliation: Division of Infection and Immunity, IBLS, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G12 8TA, United Kingdom.
authors.forename: Alison R
authors.initials: AR
authors.lastname: Kerr
authors.forename: Gavin K
authors.initials: GK
authors.lastname: Paterson
authors.forename: Jackie
authors.initials: J
authors.lastname: McCluskey
authors.forename: Francesco
authors.initials: F
authors.lastname: Iannelli
authors.forename: Marco R
authors.initials: MR
authors.lastname: Oggioni
authors.forename: Gianni
authors.initials: G
authors.lastname: Pozzi
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Mitchell
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D003165:Complement System Proteins / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D017353:Gene Deletion; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D011018:Pneumonia, Pneumococcal / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000472:pathogenicity*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D003165:Complement System Proteins