title: Live cell refractometry using microfluidic devices.
abstract: Using Hilbert phase microscopy for extracting quantitative phase images, we measured the average refractive index associated with live cells in culture. To decouple the contributions to the phase signal from the cell refractive index and thickness, we confined the cells in microchannels. The results are confirmed by comparison with measurements of spherical cells in suspension.
authors.affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Niyom
authors.initials: N
authors.lastname: Lue
authors.forename: Gabriel
authors.initials: G
authors.lastname: Popescu
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ikeda
authors.forename: Ramachandra R
authors.initials: RR
authors.lastname: Dasari
authors.forename: Kamran
authors.initials: K
authors.lastname: Badizadegan
authors.forename: Michael S
authors.initials: MS
authors.lastname: Feld
mesh_terms: D048430:Cell Shape; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques; D008858:Microscopy, Phase-Contrast; D012031:Refractometry / Q000295:instrumentation