Effect of Mongolian plants on in vivo insulin action in diabetic rats.
In the present study, we firstly performed a preliminary investigation on the acute effect of Mongolian medicinal plant extracts (Dryopteris species, Aspidiaceae) on the glucose tolerance in rats, evaluated by an oral glucose tolerance test (OGTT). Then, we investigated the effect of the same extracts on in vivo insulin action in streptozotocin (50 mg kg(-1) BW, i.v.)-induced diabetic rats by means of the euglycemic clamp. In diabetic rats, the glucose metabolic clearance rate (MCR) during 3.0 (low-dose) and 30.0 mU kg(-1)min(-1) (high-dose) insulin infusion was significantly higher in animals administered with plant extracts (500 mg kg(-1) BW, p.o.), compared with saline-administered animals (low-dose--normal control: 16.3+/-0.7 ml kg(-1)min(-1) versus plant extract 1: 15.8+/-0.6 ml kg(-1)min(-1) and plant extract 2: 14.0+/-0.5 ml kg(-1)min(-1), diabetic control: 10.9+/-0.6 ml kg(-1)min(-1) versus plant extract 1: 19.3+/-1.4 ml kg(-1)min(-1) and plant extract 2: 20.5+/-1.5 ml kg(-1)min(-1); high-dose-normal control: 50.9+/-2.6 ml kg(-1)min(-1) versus plant extract 1: 49.8+/-2.6 ml kg(-1)min(-1) and plant extract 2: 48.4+/-2.4 ml kg(-1)min(-1), diabetic control: 26.9+/-1.6 ml kg(-1)min(-1) versus plant extract 1: 33.5+/-1.7 ml kg(-1)min(-1) and plant extract 2: 37.2+/-1.9 ml kg(-1)min(-1); P<0.05, respectively). These results suggest that a single administration of the two Mongolian plant extracts can improve glucose utilization and insulin resistance in diabetic rats.
Khookhor
Oyun
O
Department of Health Science, Faculty of Psychological and Physical Science, Aichi Gakuin University, 12 Araike, Nisshin 470-0195, Japan.
Bolin
Qin
Q
Oshida
Yoshiharu
Y
Sato
Yuzo
Y
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism*; D017536:Carotid Artery, Common / Q000187:drug effects / Q000503:physiopathology*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D015309:Glucose Clamp Technique; D005951:Glucose Tolerance Test; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D007328:Insulin / Q000627:therapeutic use*; D008297:Male; D008986:Mongolia; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts