Differential expression of the pro-inflammatory cytokines IL-1beta-1, TNFalpha-1 and IL-8 in vaccinated pink (Oncorhynchus gorbuscha) and chum (Oncorhynchus keta) salmon juveniles.
Laboratory-reared pink and chum salmon juveniles (approximately 2g) received an intraperitoneal injection with a commercial, unadjuvanted Aeromonas salmonicida bacterin or sterile saline. Relative to elongation factor-1A, expression levels of genes encoding the proinflammatory cytokines interleukin-1beta-1 (IL-1beta), tumour necrosis factor-alpha-1 (TNFalpha) and interleukin-8 (IL-8) in pools of kidney and liver were examined 6- and 24-h after injection. Expression of IL-1beta was significantly elevated in pink and chum salmon by 6-h, and declined in pink salmon but not in chum salmon by 24-h. Similarly, expression of TNFalpha was significantly elevated in both species at 6h and only in chum salmon after 24-h. Expression of IL-8 was significantly elevated in both species at 6- and 24-h after injection. Expression of the three proinflammatory cytokine genes differed between salmon species both in the timing and magnitude of their expression. The significance of these differences with respect to immune function in these fish requires further research.
Fast
M D
MD
Institute for Marine Biosciences - National Research Council, 1411 Oxford St. Halifax, NS B3H 3Z1, Canada.
Johnson
S C
SC
Jones
S R M
SR
D048409:Aeromonas salmonicida / Q000276:immunology*; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression / Q000276:immunology*; D020869:Gene Expression Profiling / Q000662:veterinary; D017685:Oncorhynchus keta / Q000276:immunology*; D020648:Peptide Elongation Factor 1 / Q000032:analysis / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012474:Salmon / Q000276:immunology*
D001428:Bacterial Vaccines; D016207:Cytokines; D017931:DNA Primers; D020648:Peptide Elongation Factor 1