Simultaneous biodetoxification of S, N, and O pollutants by engineering of a carbazole-degrading gene cassette in a recombinant biocatalyst.
The gene cassette encoding enzymes responsible for degrading carbazole to anthranilic acid was introduced into a dibenzothiophene degrader. The resultant strain, Rhodococcus erythropolis XPDN, could simultaneously transform the model pollutants dibenzothiophene, carbazole, and dibenzofuran to nontoxic metabolites and may have an application potential for bioremediation.
Yu
Bo
B
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Ma
Cuiqing
C
Zhou
Wenjuan
W
Zhu
Shanshan
S
Wang
Ying
Y
Qu
Jingyao
J
Li
Fuli
F
Xu
Ping
P
D001572:Benzofurans / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002227:Carbazoles / Q000737:chemistry / Q000378:metabolism*; D004785:Environmental Pollutants / Q000737:chemistry / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D011995:Recombination, Genetic; D012240:Rhodococcus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013455:Sulfur / Q000737:chemistry / Q000378:metabolism; D013876:Thiophenes / Q000737:chemistry / Q000378:metabolism*
D001572:Benzofurans; D002227:Carbazoles; D004785:Environmental Pollutants; D013876:Thiophenes; D013455:Sulfur; C023614:dibenzofuran; D009584:Nitrogen; D010100:Oxygen; C016366:dibenzothiophene