Potent inhibition of human Hepatitis B virus replication by a host factor Vps4.
Vps4 is a host factor known to be involved in cellular vacuolar protein sorting. We report here that HBV replication and secretion can be significantly inhibited by Vps4 dominant negative, ATPase-defective, mutants K173Q and E228Q. In contrast, wild-type Vps4 at low dose can inhibit HBV replication more effectively in human hepatoblastoma cell line HepG2, than in human hepatoma cell line Huh7. To our knowledge, this is the first demonstration of an anti-HBV potential of dominant negative mutants of a protein sorting host factor Vps4.
Kian Chua
Pong
P
Institute for Human Infections and Immunology, Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA.
Lin
Min-Hui
MH
Shih
Chiaho
C
D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000502:physiology*; D019943:Amino Acid Substitution; D045744:Cell Line, Tumor; D056827:Endosomal Sorting Complexes Required for Transport; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D009154:Mutation; D012097:Repressor Proteins / Q000235:genetics* / Q000502:physiology*; D025262:Vacuolar Proton-Translocating ATPases; D033921:Vesicular Transport Proteins; D014779:Virus Replication
D056827:Endosomal Sorting Complexes Required for Transport; D012097:Repressor Proteins; D033921:Vesicular Transport Proteins; D000251:Adenosine Triphosphatases; D025262:Vacuolar Proton-Translocating ATPases; D000074183:ATPases Associated with Diverse Cellular Activities; C490305:VPS4A protein, human