Roles of BOR1, DUR3, and FPS1 in boron transport and tolerance in Saccharomyces cerevisiae.
The roles of three membrane proteins, BOR1, DUR3, and FPS1, in boron (B) transport in yeast were examined. The boron concentration in yeast cells lacking BOR1 was elevated upon exposure to 90 mM boric acid, whereas cells lacking DUR3 or FPS1 showed lower boron concentrations. Compared with control cells, cells overexpressing BOR1 or FPS1 had a lower boron concentration, and cells overexpressing DUR3 had a higher boron concentration. These results suggest that, in addition to the efflux boron transporter BOR1, DUR3 and FPS1 play important roles in regulating the cellular boron concentration. Analysis of the yeast transformants for tolerance to a high boric acid concentration revealed an apparent negative correlation between the protoplasmic boron concentration and the degree of tolerance to a high external boron concentration. Thus, BOR1, DUR3, and FPS1 appear to be involved in tolerance to boric acid and the maintenance of the protoplasmic boron concentration.
Nozawa
Akira
A
Precursory Research for Embryonic Science and Technology, Saitama, Japan.
Takano
Junpei
J
Kobayashi
Masaharu
M
von Wirén
Nicolaus
N
Fujiwara
Toru
T
D001457:Anion Exchange Protein 1, Erythrocyte / Q000502:physiology*; D001692:Biological Transport; D001888:Boric Acids / Q000494:pharmacology; D001895:Boron / Q000378:metabolism*; D003593:Cytoplasm / Q000737:chemistry; D004361:Drug Tolerance; D008565:Membrane Proteins / Q000502:physiology*; D026901:Membrane Transport Proteins; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D027981:Symporters / Q000502:physiology*
D001457:Anion Exchange Protein 1, Erythrocyte; C511808:Bor1 protein, S cerevisiae; D001888:Boric Acids; C515373:DUR3 protein, S cerevisiae; C093263:FPS1 protein, S cerevisiae; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; D001895:Boron; C032688:boric acid