Synergistic effect of anionic lipid enhancer and electroosmosis for transcutaneous delivery of insulin.
A lipid formulation consisting of 1,2-dimyristoyl-sn3-phosphatidylserine (DMPS) in a 0.2% sodium dodecylsulfate (SDS) solution was tested as an in vivo enhancer for the transcutaneous delivery of insulin. The formulation when applied to for 15 min was found to permeabilize porcine epidermis and prolong the permeable state as evidenced by electric resistance measurement. The formulation enhanced the transport of insulin through the epidermis by 40- to 100-fold, as compared to epidermis that was treated with SDS or DMPS alone. Application of electroosmosis across the formulation-treated epidermis enhanced the transport of insulin by an additional 10-fold. Pharmacokinetic studies were carried out in Sprague-Dawley rats. Transcutaneous delivery of insulin with formulation treatment and electroosmosis increased the plasma level of insulin by approximately 10-fold over delivery by formulation treatment alone. With the above protocol plasma insulin concentration remained relatively constant for up to 4h. The synergistic application of anionic lipid formulation and electroosmosis offers a promising non-invasive technique to deliver insulin transcutaneously.
Narasimha Murthy
S
S
Department of Cancer Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, United States.
Zhao
Ya-Li
YL
Hui
Sek-Wen
SW
Sen
Arindam
A
D000279:Administration, Cutaneous; D000818:Animals; D002021:Buffers; D004594:Electrophysiology; D004817:Epidermis / Q000378:metabolism; D007328:Insulin / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism; D007457:Iodine Radioisotopes / Q000627:therapeutic use; D008055:Lipids / Q000737:chemistry*; D009995:Osmosis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000473:pathology; D013552:Swine; D013997:Time Factors
D002021:Buffers; D007328:Insulin; D007457:Iodine Radioisotopes; D008055:Lipids