Quantitative determination of major active components in Ginkgo biloba dietary supplements by liquid chromatography/mass spectrometry.
A reversed-phase high-performance liquid chromatography/electrospray ionisation mass spectrometry (RP-HPLC/ESI-MS) method was developed and validated for the simultaneous determination of ten major active components in Ginkgo biloba extract (bilobalide, ginkgolides A, B, C, quercetin, kaempferol, isorhamnetin, rutin hydrate, quercetin-3-beta-D-glucoside and quercitrin hydrate) which have not been previously reported to be quantified in a single analysis. The ten components exhibit baseline separation in 50 min by C18 chromatography using a water/1:1 (v/v) methanol/acetonitrile gradient. Quantitation was performed using negative ESI-MS in selected ion monitoring (SIM) mode. Good reproducibility and recovery were obtained by this method. The sensitivity of both UV and different mass spectrometry modes (full scan, selected ion monitoring (SIM), and selected reaction monitoring (SRM)) were compared and both quantitation with and without internal standard were evaluated. The analysis of Ginkgo biloba commercial products showed remarkable variations in the rutin and quercetin content as well as the terpene lactone contents although all the products satisfy the conventional quality control method.
Ding
Shujing
S
Mass Spectrometry Research Unit, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.
Dudley
Ed
E
Plummer
Sue
S
Tang
Jiandong
J
Newton
Russell P
RP
Brenton
A Gareth
AG
D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000032:analysis; D019587:Dietary Supplements / Q000032:analysis*; D044948:Flavonols / Q000032:analysis; D005663:Furans / Q000032:analysis; D020441:Ginkgo biloba; D046934:Ginkgolides / Q000032:analysis; D044949:Kaempferols / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis*; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D012431:Rutin / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D003517:Cyclopentanes; D044948:Flavonols; D005663:Furans; D046934:Ginkgolides; D044949:Kaempferols; D010936:Plant Extracts; C099470:quercetin-3-(6''-galloyl)glucoside; C047368:3-methylquercetin; D012431:Rutin; C006552:kaempferol; D011794:Quercetin; C073710:bilobalide