title: Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp. OU01.
abstract: The chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by Microbacterium sp. OU01 in shake-flask. A significant influence of (NH(4))(2)SO(4), MgSO(4).7H(2)O and initial pH on chitosanase production was noted with Plackett-Burman design. It was then revealed with the method of steepest ascent and response surface methodology (RSM) that 19.0g/L (NH(4))(2)SO(4), 1.3g/L MgSO(4) and an initial pH of 2.0 were optimum for the production of chitosanase; colloidal chitosan appeared to be the best inducer for chitosanase production by Microbacterium sp. OU01. This optimization strategy led to the enhancement of chitosanase from 3.6U/mL to 118U/mL.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Sun
authors.forename: Baoqin
authors.initials: B
authors.lastname: Han
authors.forename: Wanshun
authors.initials: W
authors.lastname: Liu
authors.forename: Jiquan
authors.initials: J
authors.lastname: Zhang
authors.forename: Xingshuang
authors.initials: X
authors.lastname: Gao
mesh_terms: D000192:Actinomycetales / Q000201:enzymology*; D019149:Bioreactors; D003470:Culture Media / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration
chemical_list: D003470:Culture Media; D006026:Glycoside Hydrolases; C019868:chitosanase