Spectrophotometric method for quantitative determination of gliquidone in bulk drug, pharmaceutical formulations and human serum.
A quick and sensitive spectrophotometric method for quantitative determination of gliquidone in bulk drug, pharmaceutical formulations and human serum has been developed and validated. The reported method is fourteen times more sensitive than British Pharmacopoeial method. Gliquidone is a second generation sulfonyleurea derivative, indicated to improve glycemic control in NIDDM patients. The method was validated as per ICH guidelines and found advantageous for simplicity, sensitivity, reproducibility, linearity, precision and accuracy. The developed method has also been applied to the analysis of GLURENOR tablet and evaluated statistically to assess the application of the method. The limit of detection and quantification of gliquiodone at 225 nm was 68.31 ng mL-1. The, serum did not interfere with the estimation and the drug spiked in serum was totally recovered. The present method has further advantage in estimating the drug in concentrations even lower than the amount usually present in blood after oral intake. Calibration was linear in the range of 0.207-20 microg mL-1 (r2 = 0.9972). In addition, the proposed method is simple, easy to apply, low cost, requires relatively inexpensive instrument and used as an alternate to the existing spectrophotometric and non-spectrophotometric methods for the routine analysis of the drug in raw material, pharmaceutical formulations and from human serum.
Arayne
M Saeed
MS
Department of Chemistry, University of Karachi, Karachi-75270, Pakistan. arayne@gawab.com
Sultana
Najma
N
Mirza
Agha Zeeshan
AZ
D000465:Algorithms; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002853:Chromatography, Liquid; D006801:Humans; D007004:Hypoglycemic Agents / Q000032:analysis* / Q000097:blood; D007202:Indicators and Reagents; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013453:Sulfonylurea Compounds / Q000032:analysis* / Q000097:blood
D007004:Hypoglycemic Agents; D007202:Indicators and Reagents; D013453:Sulfonylurea Compounds; C010969:gliquidone