title: Integrated molecular signature of disease: analysis of influenza virus-infected macaques through functional genomics and proteomics.
abstract: Recent outbreaks of avian influenza in humans have stressed the need for an improved nonhuman primate model of influenza pathogenesis. In order to further develop a macaque model, we expanded our previous in vivo genomics experiments with influenza virus-infected macaques by focusing on the innate immune response at day 2 postinoculation and on gene expression in affected lung tissue with viral genetic material present. Finally, we sought to identify signature genes for early infection in whole blood. For these purposes, we infected six pigtailed macaques (Macaca nemestrina) with reconstructed influenza A/Texas/36/91 virus and three control animals with a sham inoculate. We sacrificed one control and two experimental animals at days 2, 4, and 7 postinfection. Lung tissue was harvested for pathology, gene expression profiling, and proteomics. Blood was collected for genomics every other day from each animal until the experimental endpoint. Gross and microscopic pathology, immunohistochemistry, viral gene expression by arrays, and/or quantitative real-time reverse transcription-PCR confirmed successful yet mild infections in all experimental animals. Genomic experiments were performed using macaque-specific oligonucleotide arrays, and high-throughput proteomics revealed the host response to infection at the mRNA and protein levels. Our data showed dramatic differences in gene expression within regions in influenza virus-induced lesions based on the presence or absence of viral mRNA. We also identified genes tightly coregulated in peripheral white blood cells and in lung tissue at day 2 postinoculation. This latter finding opens the possibility of using gene expression arrays on whole blood to detect infection after exposure but prior to onset of symptoms or shedding.
authors.affiliation: Department of Microbiology, University of Washington, Seattle, WA 98195, USA. traceyb@u.washington.edu
authors.forename: T
authors.initials: T
authors.lastname: Baas
authors.forename: C R
authors.initials: CR
authors.lastname: Baskin
authors.forename: D L
authors.initials: DL
authors.lastname: Diamond
authors.forename: A
authors.initials: A
authors.lastname: García-Sastre
authors.forename: H
authors.initials: H
authors.lastname: Bielefeldt-Ohmann
authors.forename: T M
authors.initials: TM
authors.lastname: Tumpey
authors.forename: M J
authors.initials: MJ
authors.lastname: Thomas
authors.forename: V S
authors.initials: VS
authors.lastname: Carter
authors.forename: T H
authors.initials: TH
authors.lastname: Teal
authors.forename: N
authors.initials: N
authors.lastname: Van Hoeven
authors.forename: S
authors.initials: S
authors.lastname: Proll
authors.forename: J M
authors.initials: JM
authors.lastname: Jacobs
authors.forename: Z R
authors.initials: ZR
authors.lastname: Caldwell
authors.forename: M A
authors.initials: MA
authors.lastname: Gritsenko
authors.forename: R R
authors.initials: RR
authors.lastname: Hukkanen
authors.forename: D G
authors.initials: DG
authors.lastname: Camp
authors.forename: R D
authors.initials: RD
authors.lastname: Smith
authors.forename: M G
authors.initials: MG
authors.lastname: Katze
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015870:Gene Expression; D020869:Gene Expression Profiling; D005814:Genes, Viral; D023281:Genomics; D006801:Humans; D007113:Immunity, Innate; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D007251:Influenza, Human / Q000235:genetics* / Q000276:immunology / Q000473:pathology / Q000821:virology*; D008168:Lung / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008254:Macaca nemestrina / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D008297:Male; D008954:Models, Biological; D040901:Proteomics; D013997:Time Factors