Quantitative analysis of phenobarbital in dosage form by thin-layer chromatography combined with densitometry.
In this paper, a high-performance thin-layer chromatography (HPTLC) method combined with densitometry has been described. Chromatography was performed on silica gel Si 60F254 plates using dichloromethane-ethyl acetate-formic acid (9.5 +/- 0.5 +/- 0.1, v/v) mobile phase. This method has been successfully applied for the determination of phenobarbital in pharmaceuticals. Obtained results were comparable with traditionally used column high-performance liquid chromatography (HPLC) methods. For the proposed procedure, linearity (r > 0.999), sensitivity (limit of detection 0.4 microg/spot), recovery (97.8-102.1%), and repeatability were found to be satisfactory. The HPTLC-densitometry method has many advantages, such as simplicity, reasonable sensitivity, rapidity, and low cost, and it can be successfully used in routine quality control of multidrug preparations containing barbiturates.
Wójciak-Kosior
Magdalena
M
Medical Academy, Laboratory of Planar Chromatography, Department of Chemistry, Staszica 6, 20-081, Lublin, Poland. kosiorma@wp.pl
Skalska
Agnieszka
A
Matysik
Grazyna
G
Kryska
Magdalena
M
D000085:Acetates / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry / Q000379:methods*; D004304:Dosage Forms; D005561:Formates / Q000032:analysis; D006993:Hypnotics and Sedatives / Q000032:analysis; D016014:Linear Models; D008752:Methylene Chloride / Q000032:analysis; D008956:Models, Chemical; D010634:Phenobarbital / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000085:Acetates; D004304:Dosage Forms; D005561:Formates; D006993:Hypnotics and Sedatives; C030544:formic acid; D008752:Methylene Chloride; C007650:ethyl acetate; D010634:Phenobarbital