title: Independent control of replication initiation of the two Vibrio cholerae chromosomes by DnaA and RctB.
abstract: Although the two Vibrio cholerae chromosomes initiate replication in a coordinated fashion, we show here that each chromosome appears to have a specific replication initiator. DnaA overproduction promoted overinitiation of chromosome I and not chromosome II. In contrast, overproduction of RctB, a protein that binds to the origin of replication of chromosome II, promoted overinitiation of chromosome II and not chromosome I.
authors.affiliation: Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Ave., Boston, MA 02111, USA.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Duigou
authors.forename: Kristine G
authors.initials: KG
authors.lastname: Knudsen
authors.forename: Ole
authors.initials: O
authors.lastname: Skovgaard
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Egan
authors.forename: Anders
authors.initials: A
authors.lastname: Løbner-Olesen
authors.forename: Matthew K
authors.initials: MK
authors.lastname: Waldor
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology*; D002876:Chromosomes, Bacterial / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000502:physiology; D014734:Vibrio cholerae / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria