title: Preparative isolation of hydrolysable tannins chebulagic acid and chebulinic acid from Terminalia chebula by high-speed counter-current chromatography.
abstract: As a chromatographic column, the high-speed counter-current chromatography system was equipped with a preparative HPLC series, enabling the successful isolation of hydrolysable tannins from the fruits of Terminalia chebula, a traditional Chinese medicine. The two-phase solvent system was composed of n-hexane-ethyl acetate-methanol-water (1:20:1:20 v/v). As a result, 33.2 mg chebulagic and 15.8 mg chebulinic acids were obtained in one step from 300 mg of crude extract. Their purities were determined by HPLC to be 95.3 and 96.1%, respectively. The chemical structures were identified by their MS and 1H NMR spectra.
authors.affiliation: Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Shatin N. T., Hong Kong, China.
authors.forename: Quanbin
authors.initials: Q
authors.lastname: Han
authors.forename: Jingzheng
authors.initials: J
authors.lastname: Song
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Qiao
authors.forename: Lina
authors.initials: L
authors.lastname: Wong
authors.forename: Hongxi
authors.initials: H
authors.lastname: Xu
mesh_terms: D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D047348:Hydrolyzable Tannins / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012997:Solvents; D016339:Spectrometry, Mass, Fast Atom Bombardment; D029766:Terminalia / Q000737:chemistry*
chemical_list: D001578:Benzopyrans; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D047348:Hydrolyzable Tannins; D012997:Solvents; C103481:chebulinic acid; C076178:chebulagic acid