Chemical fingerprint analysis of rhizomes of Gymnadenia conopsea by HPLC-DAD-MSn.
A high-performance liquid chromatography-diode array detection-tandem mass spectrometry (HPLC-DAD-MS(n)) method has been firstly developed for chemical fingerprint analysis of rhizomes of Gymnadenia conopsea R. Br. and rapid identification of major compounds in the fingerprints. Comparing the UV and MS spectra with those of reference compounds, seven main peaks in the fingerprints were identified as adenosine (1), 4-hydroxybenzyl alcohol (2), 4-hydroxybenzyl aldehyde (3), dactylorhin B (4), loroglossin (5), dactylorhin A (6) and militarine (7). Compounds 4-7 were succinate derivative esters and firstly discovered from this species. The Computer Aided Similarity Evaluation System (CASES) for chromatographic fingerprint of traditional Chinese medicine was employed to evaluate the similarities of 10 samples of the rhizomes of G. conopsea collected from Sichuan, Qinghai and Hebei provinces, Tibet autonomous region of China, and Nepal. These samples from different sources had similar chemical fingerprints. This method is specific and may serve for quality identification and comprehensive evaluation of this traditional Tibetan remedy.
Cai
Min
M
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; College of Life Sciences, Hubei University, Wuhan 430062, China.
Zhou
Yan
Y
Gesang
Suolang
S
Bianba
Ciren
C
Ding
Li-Sheng
LS
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D029595:Orchidaceae / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*; D019802:Succinic Acid / Q000032:analysis / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
D019802:Succinic Acid