The olfactory bulb and olfactory mucosa obtained from human cadaver donors as a source of olfactory ensheathing cells.
During the last decade, olfactory ensheathing cells (OECs) have been successfully applied in multiple experimental approaches aimed to repair damaged mammalian spinal cord. Some of these experiments have consequently been translated into clinical trials. Finding a reliable source of human OECs that is easily accessible and can ensure a sufficient number of cells is a major prerequisite for conducting studies on OEC-mediated spinal cord regeneration. Here, we present a procedure for obtaining olfactory bulbs (OBs) and olfactory mucosa (OM) simultaneously from adult cadaver heart-beating donors for OEC isolation and analyze some of the factors that may condition successful OEC culture. We show that the results of OEC culture from OBs (10 cases) correlated significantly with warm ischemia time (WIT) as well as the initial viability of the isolated cells. Efficient OEC culture was possible when the WIT for the OB was up to 20 min. Brain damage, assessed by determination of S100B serum level, was not related to the success of OEC culture from the OB. Cadaver OM (7 cases) was shown to be a more reliable source of human OECs than the OB. In most of the examined cases the efficacy of culturing OECs from cadaver OM obtained even 180 min after cardiac arrest was comparable to that of living patients. The method of obtaining OBs and OM from cadavers enables the use of an alternative source of primary adult human OECs for further preclinical and clinical studies on their neurotrophic properties.
Miedzybrodzki
Ryszard
R
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. mbrodzki@iitd.pan.wroc.pl
Tabakow
Pawel
P
Fortuna
Wojciech
W
Czapiga
Bogdan
B
Jarmundowicz
Wlodzimierz
W
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy / Q000379:methods; D016380:Brain Tissue Transplantation / Q000379:methods; D002102:Cadaver; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004210:Dissection / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000166:cytology / Q000502:physiology / Q000601:surgery; D009414:Nerve Growth Factors / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology* / Q000502:physiology; D009830:Olfactory Bulb / Q000166:cytology* / Q000502:physiology / Q000601:surgery; D009831:Olfactory Mucosa / Q000166:cytology* / Q000502:physiology / Q000601:surgery; D011180:Postmortem Changes; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism; D014019:Tissue Donors
D015415:Biomarkers; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human