Cloning and characterization of porcine Fc gamma receptor II (FcgammaRII).
Receptors for the Fc region (FcgammaRs) of IgG play a crucial role in the immune system and host protection against infection. In the present study, we describe the cloning, sequencing and characterization of porcine FcgammaRII. By screening a translated EST database with the protein sequence of the human FcgammaRII (CD32) we identified a putative porcine homologue. Using rapid amplification of cDNA ends (RACE), we isolated the full-length cDNA encoding porcine FcgammaRII from peripheral blood leucocyte RNA. The porcine FcgammaRII cDNA was 1488bp long, encoding a 297 amino acid trans-membrane glycoprotein composed of two immunoglobulin-like extracelluar domains, a trans-membrane region and a cytoplasmic tail with an immunoreceptor tyrosine-based inhibitory motif (ITIM). The predicted amino acid sequence was found to be 67% and 52% identitical with human and mouse FcgammaRIIB. RT-PCR indicated porcine FcgammaRII transcripts expressed in liver, alveolar, mesenteric lymph node and PBLs. COS-7 cells transfected with the pig FcgammaRII cDNA were able to bind chicken erythrocytes sensitized with porcine IgG. Identification of porcine FcgammaRII will aid in the understanding IgG-FcgammaR interactions, and may help in developing new immunization protocols.
Qiao
Songlin
S
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
Zhang
Gaiping
G
Xia
Chun
C
Zhang
Hong
H
Zhang
Yuyang
Y
Xi
Jun
J
Song
Haitao
H
Li
Xuewu
X
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D007074:Immunoglobulin G / Q000276:immunology; D008856:Microscopy, Fluorescence / Q000662:veterinary; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D017452:Receptors, IgG / Q000235:genetics* / Q000276:immunology*; D016415:Sequence Alignment; D013552:Swine / Q000235:genetics / Q000276:immunology*; D014162:Transfection / Q000662:veterinary
D007074:Immunoglobulin G; D012333:RNA, Messenger; D017452:Receptors, IgG