Differentiation of bone marrow mesenchymal stem cells induced by myocardial medium under hypoxic conditions.
OBJECTIVE
To explore whether bone marrow mesenchymal stem cells (MSC) can differentiate into myocardial-like cells induced by myocardial medium, especially the hypoxia/reoxygenation-conditioned medium of cardiomyocytes.


METHODS
Myocardial cells obtained from neonatal Sprague-Dawley rat ventricles were isolated and cultured in vitro and a hypoxia reoxygenation model was established. The MSC isolated from adult Sprague-Dawley rats were purified and then incubated with 3 different mediums: medium A- the conditioned medium of normal cardiomyocytes; medium B - the conditioned medium of cardiomyocytes after hypoxia reoxygenation; and the control medium - ordinary medium. The expressions of the cardiac myosin heavy chain (MHC), troponin T (TnT) and connexin 43 were investigated in the MSC after 24 h, 48 h and 72 h cultivation, respectively.


RESULTS
The MSC expressed MHC and TnT when incubated with the conditioned medium of cardiomyocytes after hypoxia reoxygenation, but did not express connexin 43. None of MHC, TnT and connexin 43 was detected in the MSC incubated with the conditioned medium of normal cardiomyocytes.


CONCLUSIONS
The results indicate for the first time that myocardial medium for hypoxic preconditioning can induce MSC differentiation into myocardial-like cells.
Xie
Xiao-jie
XJ
Division of Cardiology, the 2nd Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
Wang
Jian-an
JA
Cao
Jiang
J
Zhang
Xing
X
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000502:physiology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D018031:Connexin 43 / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020107:Troponin T / Q000378:metabolism
D018031:Connexin 43; D003470:Culture Media; D020107:Troponin T; D007770:L-Lactate Dehydrogenase; D018995:Myosin Heavy Chains; D010100:Oxygen