DNA analysis by mass spectrometry-past, present and future.
The analysis of deoxyribose nucleic acid (DNA) by mass spectrometry (MS) has evolved to where it can be used to analyze most known types of DNA and ribose nucleic acid (RNA) situations. It can efficiently deal with the analysis of DNA polymorphisms, sequences, haplotypes, human leukocyte antigen (HLA) typing, DNA methylation and RNA expression. Implementations of MS for these forms of DNA analyses are reviewed. The use of DNA analysis by MS is compared with competing technologies. Finally, an overview is given of worthwhile applications where the know-how gained so far could be used for future developments.
Tost
Jörg
J
Centre National de Génotypage, Bâtiment G2, 2 rue Gaston Crémieux, CP 5721, 91057 Evry Cedex, France.
Gut
Ivo Glynne
IG
D000818:Animals; D004247:DNA / Q000032:analysis*; D019175:DNA Methylation; D015870:Gene Expression; D023281:Genomics; D005838:Genotype; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D013058:Mass Spectrometry / Q000266:history / Q000639:trends; D020641:Polymorphism, Single Nucleotide; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000266:history / Q000639:trends
D004247:DNA