[Influence of raising oxygen content on function of platelet concentrate during preservation].
To explore the influence of raising oxygen (dissolved oxygen) content on function of platelet concentrate, the platelet concentrate was prepared by a CS-3000 plus blood cell separator. Experiments were divided into 2 groups: test group and control group. After raising oxygen content in platelet plasma under sterile operation, the platelet samples of two groups were preserved in oscillator with horizontal oscillation at 22 +/- 2 degrees C. The platelet count, platelet aggregation rate, lactic acid content and CD62p expression level of platelet were detected on 0, 1, 2, 3, 4, 5 days of platelet preservation. The results showed that the platelet count and platelet aggregation rate decreased with prolongation of preserved time, while the lactic acid content and CD62p expression level of platelet increased gradually. Compared with control group, there were significant differences in aggregation rate of platelet preserved for 2-3 days, and in CD62p expression level of platelet preserved for 1-3 days, while significant difference was found in lactic acid content of platelet preserved for 1-3 days. It is concluded that raising content of oxygen in platelet plasma can provide more oxygen to compensate oxygen supply deficiency for platelet metabolism and improve the efficiency of platelet oxygenic metabolism and the quality of platelet during preservation.
Zhan
Tong
T
Blood Center of Jiangsu Province, Nanjing 210042, China.
Xiao
Jian-Yu
JY
Tao
Jing
J
Miao
Xi-Feng
XF
Liu
Yan-Cun
YC
Tang
Rong-Cai
RC
D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D001793:Blood Preservation / Q000379:methods*; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010976:Platelet Count; D010979:Platelet Function Tests
D019344:Lactic Acid; D010100:Oxygen