title: A novel extended-spectrum TEM-type beta-lactamase, TEM-138, from Salmonella enterica serovar Infantis.
abstract: A novel natural TEM beta-lactamase with extended-spectrum activity, TEM-138, was identified in a ceftazidime-resistant clinical isolate of Salmonella enterica serovar Infantis. Compared to TEM-1, TEM-138 contains the following mutations: E104K, N175I, and G238S. The bla(TEM-138) gene was located on a 50-kb transferable plasmid. Expression studies with Escherichia coli revealed efficient ceftazidimase and cefotaximase activities for TEM-138.
authors.affiliation: Laboratoire de Biochimie et de Biotechnologie, Faculté des Sciences de Tunis, 2092 El-Manar II, Tunisia.
authors.forename: Chedly
authors.initials: C
authors.lastname: Chouchani
authors.forename: Renaud
authors.initials: R
authors.lastname: Berlemont
authors.forename: Afef
authors.initials: A
authors.lastname: Masmoudi
authors.forename: Moreno
authors.initials: M
authors.lastname: Galleni
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Frere
authors.forename: Omrane
authors.initials: O
authors.lastname: Belhadj
authors.forename: Kamel
authors.initials: K
authors.lastname: Ben-Mahrez
mesh_terms: D001483:Base Sequence; D002442:Ceftazidime / Q000494:pharmacology; D018550:Cephalosporin Resistance; D006801:Humans; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D019779:Salmonella enterica / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D002442:Ceftazidime; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1