Differentiation of hepatocytoid cell induced from whole-bone-marrow method isolated rat myeloid mesenchymal stem cells.
OBJECTIVE
To explore the expansion and differentiation of hepatocytoid cell induced from myeloid mesenchymal stem cell (MSC) in vitro, in order to find suitable resource of hepatocytes for bioartificial liver or liver transplantation.


METHODS
The rat myeloid MSC was isolated and divided into three groups which were cultured by Friedensteion method, and then were induced by culture fluid, culture fluid plus cholestatic serum and culture fluid plus hepatocyte growth factor (HGF), respectively. Hepatocytoid cell as well as expression of CK18 and AFP was observed by immunohistochemistry.


RESULTS
After the induction for 21 d, hepatocytoid cell was observed, and its expression of CK18 and AFP was detected by immunohistochemistry in MSC cultured with cholestatic serum. Furthermore, on the 35th d, albumin mRNA was expressed in the cell, suggesting the inducing effect was similar to that by HGF.


CONCLUSIONS
Rat myeloid MSC can differentiate into hepatocyte lineage under appropriate condition. This method is easy to operate.
Li
Wei
W
Department of Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. lsnlw@sina.com
Liu
Su-Nan
SN
Luo
Duan-De
DD
Zhao
Lei
L
Zeng
Ling-Lan
LL
Zhang
Shu-Ling
SL
Li
Shu-Li
SL
D000418:Albumins / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology; D053538:Keratin-18 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D000509:alpha-Fetoproteins / Q000378:metabolism
D000418:Albumins; D053538:Keratin-18; D000509:alpha-Fetoproteins