Independent control of replication initiation of the two Vibrio cholerae chromosomes by DnaA and RctB.
Although the two Vibrio cholerae chromosomes initiate replication in a coordinated fashion, we show here that each chromosome appears to have a specific replication initiator. DnaA overproduction promoted overinitiation of chromosome I and not chromosome II. In contrast, overproduction of RctB, a protein that binds to the origin of replication of chromosome II, promoted overinitiation of chromosome II and not chromosome I.
Duigou
Stéphane
S
Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Ave., Boston, MA 02111, USA.
Knudsen
Kristine G
KG
Skovgaard
Ole
O
Egan
Elizabeth S
ES
Løbner-Olesen
Anders
A
Waldor
Matthew K
MK
D001426:Bacterial Proteins / Q000502:physiology*; D002876:Chromosomes, Bacterial / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000502:physiology; D014734:Vibrio cholerae / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria