Direct and random routing of a molecular motor protein at a DNA junction.
With the aim of investigating how motor proteins negotiate DNA nanostructures, we produced test circuits based on recombination intermediates in which 1D translocation across a Holliday junction (HJ) could be assessed by subsequent triplex displacement signals on each DNA arm. Using the EcoR124I restriction-modification enzyme, a 3'-5' double-strand DNA (dsDNA) translocase, we could show that the motor will tend to follow its translocated strand across a junction. Nonetheless, as the frequency of junction bypass events increases, the motor will occasionally jump tracks.
Stanley
Louise K
LK
DNA-Protein Interactions Unit, Department of Biochemistry, University of Bristol, Bristol, BS8 1TD, UK.
Szczelkun
Mark D
MD
D045566:DNA, Cruciform / Q000737:chemistry* / Q000378:metabolism; D015253:Deoxyribonucleases, Type I Site-Specific / Q000378:metabolism*; D020409:Molecular Motor Proteins / Q000378:metabolism*; D009038:Motion; D009690:Nucleic Acid Conformation
D045566:DNA, Cruciform; D020409:Molecular Motor Proteins; C048242:endodeoxyribonuclease EcoR124I; D015253:Deoxyribonucleases, Type I Site-Specific