Prior antibacterial peptide-mediated inhibition of protein folding in bacteria mutes resistance enzymes.
The antimicrobial activity of amoxicillin against TEM-1-expressing strains could be fully recovered when bacteria were preincubated with sublethal doses of an antibacterial peptide derivative. Assays with the simultaneous administration of antibiotics or synergy assays with kanamycin or ciprofloxacin, where resistance development does not involve properly folded proteins, failed to yield similar results.
Otvos
Laszlo
L
Sbarro Institute for Cancer Research and Molecular Medicine, Temple University, Philadelphia, PA 19122, USA. otvos@temple.edu
de Olivier Inacio
Vanessa
V
Wade
John D
JD
Cudic
Predrag
P
D000658:Amoxicillin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D004357:Drug Synergism; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D019476:Insect Proteins / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D017510:Protein Folding; D001618:beta-Lactamases / Q000096:biosynthesis / Q000378:metabolism
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D029968:Escherichia coli Proteins; D019476:Insect Proteins; C087710:pyrrhocoricin protein, Pyrrhocoris apterus; D000658:Amoxicillin; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1