title: Relationship between N-acetyl transferase-2 gene polymorphism and risk of bronchial asthma.
abstract: There are still uncertainties as to the mechanism of many pathological conditions, among them allergic diseases. It has been suggest that acetylation rate may be a factor that influences the development of allergic diseases. The aim of the present study was to investigate further whether the genetic polymorphism of the NAT2 plays a role in susceptibility to bronchial asthma disease. Ninety-seven patients with bronchial asthma (atopic n= 62; non-atopic n= 35) and 104 healthy individuals were participated in this study. DNA was extracted from the leucocyte by high pure template preparation kit. NAT2*5A, NAT2*6A, NAT2*7A/B and NAT2*14A polymorphisms of NAT2 were detected by using LightCycler-NAT2 mutation detection kit by real time PCR with LightCycler instrument. We found that mutant NAT2*5A (OR= 3.84, 95% CI= 1.08-13.6) and NAT2*6A (OR= 5.27, 95% CI= 1.06-26.05) genotype could be associated with a high risk for the development of bronchial asthma according to the genotype. After grouping phenotype, the risk for bronchial asthma was more than two times higher (OR= 2.7, 95% CI= 1.07-6.97) in individuals with the slow NAT2*5A acetylator phenotype compared to the fast phenotype. Our study suggests that the NAT2 slow acetylators may be a determinant in susceptibility to asthma disease. This finding may have implications for the theories for the pathogenesis of the disease as well as for therapeutic aspects.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Mersin University, Mersin, Turkey. lutamer@yahoo.com
authors.forename: Lülüfer
authors.initials: L
authors.lastname: Tamer
authors.forename: Mukadder
authors.initials: M
authors.lastname: Calikoğlu
authors.forename: Nurcan
authors.initials: N
authors.lastname: Aras Ateş
authors.forename: Hatice
authors.initials: H
authors.lastname: Yildirim
authors.forename: Sevim
authors.initials: S
authors.lastname: Karakaş
authors.forename: Uğur
authors.initials: U
authors.lastname: Atik
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D001249:Asthma / Q000209:etiology / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011110:Polymorphism, Genetic; D012307:Risk Factors; D014421:Turkey; D044465:White People / Q000235:genetics
chemical_list: D000123:Acetyltransferases