Pork peptone stimulates cholecystokinin secretion from enteroendocrine cells and suppresses appetite in rats.
We found that soybean beta-conglycinin peptone (BconP) suppresses food intake through cholecystokinin (CCK) release from enteroendocrine cells in association with binding of the peptone to rat small intestinal brush border membrane (BBM). The aim of the present study was to find new appetite suppressing peptides. Peptones from chicken, pork, beef, beef liver, and egg white were examined for activities to bind with rat BBM, CCK-release from enteroendocrine cell line STC-1, and induce satiety in rats. Chicken and pork peptone (ChickP and PorkP) bound to BBM with highest ability as evaluated with a surface plasmon biosensor. PorkP and ChickP released CCK in higher amounts than BconP from STC-1 cells dose-dependently, with highest stimulation by PorkP. An orogastric preload of PorkP, but not ChickP, suppressed food intake similarly to BconP, dose-dependently. These results suggest that PorkP interacts directly with the small intestinal CCK cells to release CCK, and that it suppresses appetite in rats.
Sufian
M K N B
MK
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Hira
Tohru
T
Miyashita
Kyoko
K
Nishi
Takashi
T
Asano
Kozo
K
Hara
Hiroshi
H
D000818:Animals; D001066:Appetite / Q000187:drug effects*; D002417:Cattle; D002645:Chickens; D002766:Cholecystokinin / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004435:Eating; D019858:Enteroendocrine Cells / Q000737:chemistry / Q000187:drug effects*; D007421:Intestine, Small / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010461:Peptones / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D013552:Swine
D010461:Peptones; D002766:Cholecystokinin