Determination of abamectin, doramectin, emamectin, eprinomectin, ivermectin, and moxidectin in milk by liquid chromatography electrospray tandem mass specrometry.
The avermectin and milbemycin families of compounds are derived from naturally occurring yeasts. They have proven to be potent preventatives against a variety of pests such as insects and parasites. Only eprinomectin and moxidectin are currently approved for use on lactating cattle with tolerances in milk of 12 microg/kg for eprinomectin and 40 microg/kg for moxidectin. Detection of misuse or inadvertent contamination in milk requires a sensitive and definitive analytical method. A method has been developed for the determination of 5 avermectins and 1 milbemycin in milk using a simple liquid-liquid extraction and liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis. Ivermectin (IVR), doramectin (DOR), abamectin (ABA), eprinomectin (EPR), emamectin (EMA), and moxidectin (MOX) were extracted from whole milk by partitioning into acetonitrile with a subsequent solvent exchange into methanol-water. Simultaneous confirmation and quantification were achieved with LC separation, positive electrospray ionization (ESI+), and MS/MS. The limits of detection ranged from 16 pg/g (ppt) for EMA to 1.7 microg/g (ppb) for MOX.
Sheridan
Robert
R
New York State Department of Agriculture and Markets, Food Laboratory Bldg 7, 3rd Floor, State Office Campus, Albany, NY 12235, USA. robert.sheridan@agmkt.state.ny.us
Desjardins
Lucille
L
D000097:Acetonitriles / Q000032:analysis; D000818:Animals; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004187:Disaccharides / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D007306:Insecticides / Q000032:analysis*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis*; D018942:Macrolides / Q000032:analysis; D008892:Milk / Q000378:metabolism*; D012997:Solvents / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014867:Water / Q000032:analysis
D000097:Acetonitriles; D004187:Disaccharides; D007306:Insecticides; D018942:Macrolides; D012997:Solvents; D014867:Water; C048324:abamectin; D007559:Ivermectin; C101434:eprinomectin; C443359:emamectin; C084101:doramectin; C027837:moxidectin; C032159:acetonitrile