Storage of spatial information by the maintenance mechanism of LTP.
Analogous to learning and memory storage, long-term potentiation (LTP) is divided into induction and maintenance phases. Testing the hypothesis that the mechanism of LTP maintenance stores information requires reversing this mechanism in vivo and finding out whether long-term stored information is lost. This was not previously possible. Recently however, persistent phosphorylation by the atypical protein kinase C isoform, protein kinase Mzeta (PKMz), has been found to maintain late LTP in hippocampal slices. Here we show that a cell-permeable PKMz inhibitor, injected in the rat hippocampus, both reverses LTP maintenance in vivo and produces persistent loss of 1-day-old spatial information. Thus, the mechanism maintaining LTP sustains spatial memory.
Pastalkova
Eva
E
Departments of Physiology, Pharmacology, and Neurology, Robert F. Furchgott Center for Neural and Behavioral Science, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA.
Serrano
Peter
P
Pinkhasova
Deana
D
Wallace
Emma
E
Fenton
André Antonio
AA
Sacktor
Todd Charlton
TC
D000818:Animals; D001362:Avoidance Learning; D003213:Conditioning, Psychological; D018891:Dentate Gyrus / Q000187:drug effects / Q000502:physiology; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000008:administration & dosage / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008568:Memory / Q000187:drug effects / Q000502:physiology*; D019580:Perforant Pathway; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D000071456:Sequestosome-1 Protein; D019311:Staurosporine / Q000494:pharmacology; D013997:Time Factors
D006360:Heat-Shock Proteins; D000071456:Sequestosome-1 Protein; C491079:Sqstm1 protein, rat; D011493:Protein Kinase C; C504730:protein kinase M zeta, rat; D019311:Staurosporine