Identification of a gene from the arbuscular mycorrhizal fungus Glomus intraradices encoding for a 14-3-3 protein that is up-regulated by drought stress during the AM symbiosis.
In the present study, a 14-3-3 protein-encoding gene from Glomus intraradices has been identified after differential hybridization of a cDNA library constructed from the fungus growing in vitro and subjected to drought stress by addition of 25% PEG 6000. Subsequently, we have studied its expression pattern under drought stress in vitro and also when forming natural symbioses with different host plants. The results obtained suggest that Gi14-3-3 gene may be involved in the protection that the arbuscular mycorrhizal (AM) symbiosis confers to the host plant against drought stress. Our findings provide new evidences that the contribution of AM fungi to the enhanced drought tolerance of the host plant can be mediated by a group of proteins (the 14-3-3) that regulate both signaling pathways and also effector proteins involved in the final plant responses.
Porcel
Rosa
R
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), Prof. Albareda, 1, 18008, Granada, Spain.
Aroca
Ricardo
R
Cano
Custodia
C
Bago
Alberto
A
Ruiz-Lozano
Juan Manuel
JM
D048948:14-3-3 Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000222:Adaptation, Physiological; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004190:Disasters; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D018545:Lactuca / Q000382:microbiology; D008969:Molecular Sequence Data; D038821:Mycorrhizae / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012988:Soil Microbiology; D013025:Glycine max / Q000382:microbiology; D013559:Symbiosis; D014026:Nicotiana / Q000382:microbiology; D015854:Up-Regulation; D003313:Zea mays / Q000382:microbiology
D048948:14-3-3 Proteins; D018076:DNA, Complementary; D005656:Fungal Proteins