[DNA microarrays and their application in detecting and identifying intestinal pathogens].
DNA microarrays offer many advantages of high throughout, automation, rapid detection, and so on. Therefore, this technology had been used in many fields such as molecular epidemiology of bacteria, microbial gene identification, disease mechanism, gene mutation, gene expression identification, DNA sequencing and medicine screening etc. The assays for identifying pathogens using DNA microarrays reported aboard recently are introduced. The application of DNA microarrays in detecting and identifying intestinal pathogens mainly includes three aspects: the identification of toxin and characteristic genes of pathogens, the identification of bacterial DNA or RNA directly, the simultaneous detection of a large number of intestinal pathogens with the target - gene of ribosomal RNA. Because of its high efficiency, DNA microarrays is superior to other biological method. Obviously DNA microarrays technology may be useful in identifying intestinal pathogens and have a wide prospect.
Jin
Da-Zhi
DZ
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China. dazhijin@163.com
Wen
Si-Yuan
SY
Wang
Sheng-Qi
SQ
D000818:Animals; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D012329:RNA, Bacterial