[Determination of orlistat in health food for controlling body weight by high performance liquid chromatography/electrospray spectrometry].
OBJECTIVE
To establish a method of determining orlistat in health food by on line HPLC-UV-ESI/MS.


METHODS
The separation was completed on an analytical Spherigel C8 column (5 microm, 200 mm x 4.6 mm) with acetonitrile (0.1% formic acid) : water (0.1% formic acid) = 80 : 20 as mobile phase, the detection wavelength was 2003 mm.


RESULTS
Good linearity between peak areas and concentrations was obtained during rag of 0.3 - 0.6 mg/ml.


CONCLUSIONS
The method is simple and can be used to determine orlistat in the health food for controlling body weight accurately.
Xiao
Song
S
Analytical and Testing Center of Human Normal University, Changsha 410081, China.
Zhu
Xiao-Lan
XL
Chen
Bo
B
Yao
Shou-Zhuo
SZ
D019440:Anti-Obesity Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D006801:Humans; D007783:Lactones / Q000032:analysis*; D000077403:Orlistat; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D019440:Anti-Obesity Agents; D007783:Lactones; D000077403:Orlistat