Improved technique that allows the performance of large-scale SNP genotyping on DNA immobilized by FTA technology.
FTA technology is a novel method designed to simplify the collection, shipment, archiving and purification of nucleic acids from a wide variety of biological sources. The number of punches that can normally be obtained from a single specimen card are often however, insufficient for the testing of the large numbers of loci required to identify genetic factors that control human susceptibility or resistance to multifactorial diseases. In this study, we propose an improved technique to perform large-scale SNP genotyping. We applied a whole genome amplification method to amplify DNA from buccal cell samples stabilized using FTA technology. The results show that using the improved technique it is possible to perform up to 15,000 genotypes from one buccal cell sample. Furthermore, the procedure is simple. We consider this improved technique to be a promising methods for performing large-scale SNP genotyping because the FTA technology simplifies the collection, shipment, archiving and purification of DNA, while whole genome amplification of FTA card bound DNA produces sufficient material for the determination of thousands of SNP genotypes.
He
Hongbin
H
INSERM U399, Immunology and Genetics of Parasitic Diseases, Laboratory of Parasitology-Mycology, Faculty of Medicine, University of Méditerranée, Marseille, France.
Argiro
Laurent
L
Dessein
Helia
H
Chevillard
Christophe
C
D016172:DNA Fingerprinting; D005821:Genetic Techniques; D016678:Genome; D005838:Genotype; D006801:Humans; D009061:Mouth Mucosa; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide