title: Isolation and characterization of full-length recombinant cattle PrPC protein.
abstract: Full-length Bos taurus PrPC protein was obtained in the eu- and prokaryotic expression systems. Immunoblotting and indirect enzyme immunoassay demonstrated high specificity and antigenic activity of full-length proteins in the reactions with monoclonal antibodies (anti-SAF-32 and VRQ-84). Membrane location of recombinant PrPC protein in insect cells was shown by immunofluorescent analysis.
authors.affiliation: D I Ivanovsky Institute of Virology, Russian Academy of Medical Sciences. kalnov@narvac.com
authors.forename: S L
authors.initials: SL
authors.lastname: Kal'nov
authors.forename: V B
authors.initials: VB
authors.lastname: Grigor'ev
authors.forename: K P
authors.initials: KP
authors.lastname: Alekseev
authors.forename: A N
authors.initials: AN
authors.lastname: Vlasova
authors.forename: R A
authors.initials: RA
authors.lastname: Gibadulin
authors.forename: A N
authors.initials: AN
authors.lastname: Pokidyshev
authors.forename: M V
authors.initials: MV
authors.lastname: Balandina
authors.forename: V V
authors.initials: VV
authors.lastname: Tsibezov
authors.forename: O A
authors.initials: OA
authors.lastname: Verkhovskii
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000032:analysis; D016367:Baculoviridae / Q000235:genetics; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry; D018621:PrPC Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D018621:PrPC Proteins; D011994:Recombinant Proteins