title: [The concentration and expression of IL-4, IL-5, IL-6, IL-8 in human nasal polyps tissues].
abstract: OBJECTIVE
  To study the concentration and expression of IL-4, IL-5, IL-6, IL-8 in human nasal polyps tissues, and to explore the relationship between cytokines and the formative mechanism of nasal polyps.
  
  
  METHODS
  The concentration and expression of IL-4, IL-5, IL-6, IL-8 in 54 cases with nasal polyps were determined by enzyme linked immunosorbent assay (ELISA) and immunohistochemistry, and the middle turbinate mucosa from 22 patients with the deviation of nasal septum were used as the control.
  
  
  RESULTS
  The concentration of IL-5, IL-8 in nasal polyps tissues were significantly higher than that in normal middle turbinate mucosa (P < 0.01). There was no significant difference between the concentration of IL-6 in nasal polyps and that in normal control group (P > 0.05). No significant difference was found between the concentration of IL-4 in nasal polyps tissues with negative allergic skin test and that in control group, but the concentration of IL-4 was significantly higher in nasal polyps tissues with positive allergic skin test than in control group (P < 0.05). IL-4 was mostly found in lymphocytes and plasma cells in nasal polyps and IL-5 in eosinophils and lymphocytes. IL-6 and IL-8 were mostly found in nasal polyps epithelium and inflammatory cells, as was no difference between positive allergic skin test and negative one.
  
  
  CONCLUSIONS
  IL-5 and IL-8 are the key cytokines in the formation of nasal polyps, and IL-4 is a key cytokine in nasal polyps with positive allergic skin test. IL-6 isn't an important cytokine in the formative mechanism of nasal polyps.
authors.affiliation: Department of Otolaryngology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China. yjxthfll@hotmail.com
authors.forename: Jianxin
authors.initials: J
authors.lastname: Yue
authors.forename: Jianjun
authors.initials: J
authors.lastname: Chen
authors.forename: Weijia
authors.initials: W
authors.lastname: Kong
authors.forename: Hui
authors.initials: H
authors.lastname: Tan
authors.forename: Hong
authors.initials: H
authors.lastname: Shu
authors.forename: Qiumei
authors.initials: Q
authors.lastname: Shi
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism*; D015848:Interleukin-5 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism*; D055815:Young Adult
chemical_list: C508606:CXCL8 protein, human; C508598:IL4 protein, human; C508599:IL5 protein, human; C508600:IL6 protein, human; D015848:Interleukin-5; D015850:Interleukin-6; D016209:Interleukin-8; D015847:Interleukin-4