CMRF-35-like molecule-5 constitutes novel paired receptors, with CMRF-35-like molecule-1, to transduce activation signal upon association with FcRgamma.
The murine CMRF-35-like molecule (CLM) family belongs to the Ig superfamily, and consists of nine mapped genes. Here we report that CLM-5 and CLM-1 constitute novel paired Ig-like receptors (PIRs). CLM-1 and CLM-5 genes are encoded in close vicinity on chromosome 11. CLM-1 has been reported to possess immunoreceptor tyrosine-based inhibitory motifs in its cytoplasmic region and to inhibit the differentiation of osteoclast. CLM-5 has an Ig domain that is highly homologous with that of CLM-1 in its extracellular domain, and possesses a negatively charged residue in its transmembrane domain. CLM-5, like CLM-1, is expressed in myeloid cells, such as dendritic cells, macrophages and granulocytes. We also show that CLM-5 interacts with FcRgamma to be expressed on the plasma membrane and that the cross-linking of CLM-5 leads to tyrosine phosphorylation of proteins, including FcRgamma, in the monocyte/macrophage cell line. Taken together, these characteristics suggest that CLM-1 and CLM-5 constitute novel PIRs and that CLM-5 may transduce activation signals as the activating receptor in myeloid cells.
Fujimoto
Masanori
M
Laboratory for Epithelial Immunobiology, Research Center for Allergy and Immunology, RIKEN, Yokohama 230-0045, Japan.
Takatsu
Hiroyuki
H
Ohno
Hiroshi
H
D020816:Amino Acid Motifs / Q000235:genetics / Q000276:immunology; D000818:Animals; D002875:Chromosomes / Q000235:genetics* / Q000276:immunology; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology*; D051379:Mice; D022423:Myeloid Cells / Q000166:cytology / Q000276:immunology; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational / Q000235:genetics / Q000276:immunology; D011940:Receptor Aggregation / Q000235:genetics / Q000276:immunology*; D017452:Receptors, IgG / Q000235:genetics / Q000276:immunology*; D011971:Receptors, Immunologic / Q000235:genetics* / Q000276:immunology; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*
C482560:CLM-1 protein, mouse; C516040:Cd300ld protein, mouse; D017452:Receptors, IgG; D011971:Receptors, Immunologic