[Comparison the screening methods of the acute toxicity in vitro].
OBJECTIVE
To provide the proof for establishing the high throughput screening method of the acute toxicity by comparing the 4 screening methods of the acute toxicity in vitro.


METHODS
HepG2 cells, plated in 96-well plates, were treated with 47 compounds and we detected the cytotoxicity using 4 methods and calculated the IC50, IC45, IC40, IC35, IC30, and these values were compared with the LD50.


RESULTS
The IC45 from the neutral red method has the best relativity with the LD50.


CONCLUSIONS
Plating HepG2 cells in 96-well plates, the cytotoxicity be detected using the neutral red method and the IC45 was calculated. It is considered to provide the proof for establishing the high throughput screening method of the acute toxicity in vitro.
Wang
Zheng
Z
Department of Toxicology, Second Military Medical University, Shanghai 200433, China.
Zhang
Tian-Bao
TB
Zhu
Yu-Ping
YP
Xie
Zong-Hua
ZH
D002470:Cell Survival / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D007928:Lethal Dose 50; D009499:Neutral Red; D023382:Toxicity Tests, Acute / Q000379:methods*
D009499:Neutral Red