Production and characterization of thermostable alpha-amylase by thermophilic Geobacillus stearothermophilus.
Studies on the alpha-amylase-producing thermophilic bacterium isolated and identified from a hot spring in Jordan and designated as Geobacillus stearothermophilus JT2 were carried out. The optimum conditions for growth and enzyme production were pH 7 and 55 degrees C. The study of the kinetics of cellular growth indicated a mu(max) of 0.22/h, a K(s) of 1.2 g/L, a tau(d) of 3.15 h and a Y(x/s) of 0.43 g cell/g starch. In addition, the activation energy for growth and death were estimated and found to be 30.5 and 210 J/mol, respectively. The effect of different carbon and nitrogen sources on the cellular growth was tested.
Al-Qodah
Zakaria
Z
Department of Chemical Engineering, Al-Balqaa Applied University, Amman, Marka, Jordan. zakaria_al_qodah@fet.edu.jo
D002244:Carbon / Q000378:metabolism*; D049109:Cell Proliferation; D004795:Enzyme Stability; D001411:Geobacillus stearothermophilus / Q000254:growth & development* / Q000378:metabolism*; D045302:Geobacter / Q000254:growth & development / Q000378:metabolism; D007597:Jordan; D007700:Kinetics; D009584:Nitrogen / Q000378:metabolism*; D013045:Species Specificity; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000737:chemistry*
D002244:Carbon; D000516:alpha-Amylases; D009584:Nitrogen