title: Neonatal and adult CD4+ CD3- cells share similar gene expression profile, and neonatal cells up-regulate OX40 ligand in response to TL1A (TNFSF15).
abstract: We report here the quantitative expression of a set of immunity-related genes, including TNF family members, chemokine receptors, and transcription factors, in a CD4+ CD3- accessory cell. By correlating gene expression between cell-sorted populations of defined phenotype, we show that the genetic fingerprint of these CD4+ CD3- cells is distinct from dendritic cells, plasmacytoid dendritic cells, T cells, B cells, and NK cells. In contrast, it is highly similar to CD4+ CD3- cells isolated from embryonic and neonatal tissues, with the exception that only adult populations express OX40L and CD30L. We have previously reported that IL-7 signals regulate CD30L expression. In the present study, we show that both neonatal and adult CD4+ CD3- cells express the TNF family member, death receptor 3 (TNFRSF25), and that addition of TL1A (TNFSF15), the ligand for death receptor 3, up-regulates OX40L on neonatal CD4+ CD3- cells. Finally, we demonstrate that this differentiation occurs in vivo: neonatal CD4+ CD3- cells up-regulate both CD30L and OX40L after adoptive transfer into an adult recipient.
authors.affiliation: Medical Research Council Centre for Immune Regulation, Institute for Biomedical Research, Birmingham Medical School, Birmingham, United Kingdom.
authors.forename: Mi-Yeon
authors.initials: MY
authors.lastname: Kim
authors.forename: Kai-Michael
authors.initials: KM
authors.lastname: Toellner
authors.forename: Andrea
authors.initials: A
authors.lastname: White
authors.forename: Fiona M
authors.initials: FM
authors.lastname: McConnell
authors.forename: Fabrina M C
authors.initials: FM
authors.lastname: Gaspal
authors.forename: Sonia M
authors.initials: SM
authors.lastname: Parnell
authors.forename: Eric
authors.initials: E
authors.lastname: Jenkinson
authors.forename: Graham
authors.initials: G
authors.lastname: Anderson
authors.forename: Peter J L
authors.initials: PJ
authors.lastname: Lane
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000235:genetics; D017252:CD3 Complex / Q000378:metabolism*; D053285:CD30 Ligand; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002478:Cells, Cultured; D016172:DNA Fingerprinting; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D053278:OX40 Ligand; D012333:RNA, Messenger / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000235:genetics; D011988:Receptors, Thyroid Hormone / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism; D053219:Receptors, Tumor Necrosis Factor, Member 25; D015398:Signal Transduction; D013154:Spleen / Q000378:metabolism; D053380:Tumor Necrosis Factor Ligand Superfamily Member 15; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D048069:Tumor Necrosis Factors / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D015703:Antigens, CD; D017252:CD3 Complex; D053285:CD30 Ligand; D008562:Membrane Glycoproteins; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D053278:OX40 Ligand; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D011988:Receptors, Thyroid Hormone; D018124:Receptors, Tumor Necrosis Factor; D053219:Receptors, Tumor Necrosis Factor, Member 25; C539901:Rorc protein, mouse; C506034:Tnfrsf25 protein, mouse; C505947:Tnfsf15 protein, mouse; C504586:Tnfsf4 protein, mouse; C505775:Tnfsf8 protein, mouse; D053380:Tumor Necrosis Factor Ligand Superfamily Member 15; D014409:Tumor Necrosis Factor-alpha; D048069:Tumor Necrosis Factors