Biotransformation of isoeugenol to vanillin by Pseudomonas putida IE27 cells.
The ability to produce vanillin and/or vanillic acid from isoeugenol was screened using resting cells of various bacteria. The vanillin- and/or vanillic-acid-producing activities were observed in strains belonging to the genera Achromobacter, Aeromonas, Agrobacerium, Alcaligenes, Arthrobacter, Bacillus, Micrococcus, Pseudomonas, Rhodobacter, and Rhodococcus. Strain IE27, a soil isolate showing the highest vanillin-producing activity, was identified as Pseudomonas putida. We optimized the culture and reaction conditions for vanillin production from isoeugenol using P. putida IE27 cells. The vanillin-producing activity was induced by adding isoeugenol to the culture medium but not vanillin or eugenol. Under the optimized reaction conditions, P. putida IE27 cells produced 16.1 g/l vanillin from 150 mM isoeugenol, with a molar conversion yield of 71% at 20 degrees C after a 24-h incubation in the presence of 10% (v/v) dimethyl sulfoxide.
Yamada
Mamoru
M
Department of Biomolecular Science, Gifu University, Yanagido 1-1, 501-1193, Gifu, Japan.
Okada
Yukiyoshi
Y
Yoshida
Toyokazu
T
Nagasawa
Toru
T
D001547:Benzaldehydes / Q000378:metabolism*; D001711:Biotransformation; D005054:Eugenol / Q000031:analogs & derivatives* / Q000378:metabolism; D005285:Fermentation; D016958:Pseudomonas putida / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014641:Vanillic Acid / Q000378:metabolism
D001547:Benzaldehydes; D005054:Eugenol; C036643:isoeugenol; C100058:vanillin; D014641:Vanillic Acid