title: [Flow fluorimetry in differential diagnosis of diffuse large B-cell lymphoma].
abstract: OBJECTIVE
  To analyse immunophenotype of diffuse large B-cell lymphoma (DLBCL) with flow cytometry.
  
  
  METHODS
  Combinations of antibodies against the following antigens were used: CD3/ CD19/CD45, CD5/CD19/CD38, CD19/CD10/CD23, CD4/CD8/CD3, kappa/lambda/CD19, CD25/CD20/FMC7; CD43/CD22/CD20; CD79a/Ki-67/CD3; cytoplasmic kappa/lambda. The analysis was made on flow fluorimeter FacsCalibur using computer program CellQuest (Beckton Dickenson, USA).
  
  
  RESULTS
  Specific coexpression of markers is not detectable in DLBCL, in the greatest degree the phenotype corresponds to lymphoma from the cells of the marginal zone. The study of cells with maximal direct light diffusion provides more precise assessment of clonality and proliferative potential of tumor cells than the analysis of the whole lymphocytic polygon. The proliferative index in 33 cases of DLBCL varied in the range 10-60%.
  
  
  CONCLUSIONS
  Flow cytometry in most DLBCL cases allows identification of B-cell clonality, more precise assessment of a proliferative potential of the tumor.
authors.forename: I A
authors.initials: IA
authors.lastname: Vorob'ev
authors.forename: L A
authors.initials: LA
authors.lastname: Gorgidze
authors.forename: E M
authors.initials: EM
authors.lastname: Gretsov
authors.forename: E P
authors.initials: EP
authors.lastname: Korneva
authors.forename: D V
authors.initials: DV
authors.lastname: Kharazishvili
authors.forename: O A
authors.initials: OA
authors.lastname: Khudoleeva
authors.forename: Zh V
authors.initials: ZhV
authors.lastname: Churakova
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D003937:Diagnosis, Differential; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D016393:Lymphoma, B-Cell / Q000473:pathology*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology*
chemical_list: D000951:Antigens, Neoplasm