[Separation and purification of phosphatidylcholine in swine liver and its inhibition effect on proliferation of rat hepatoma cells].
Phosphatidylcholine (PC) in crude phospholipids from swine liver was separated and purified by using Al2O3 column chromatography with 95% alcohol as eluent. The purity was determined by thin layer chromatography on GF254 silica gel plate and with chloroform-methanol-water (65:25:4,v/v) as developing agent. The results showed that PC was completely separated from phosphatidylethanolamine (PE) by the elution with 95% alcohol, and its purity and yield reached more than 90% and 80% with a elution volume of 225 mL, and 87.6% and 87.3% with a elution volume of 425 mL respectively. The effect of the PC with different concentrations on the proliferation of rat hepatoma cell line (CBRH-7919) was determined by microculture tetrazolium (MTT) assays in vitro and was compared with that of human leukemia cell line K562. Result shows that the PC derived from the liver inhibited the growth of CBRH-7919 cells significantly. It suggested that PC derived from animal liver might function as a specific inhibitor for hepatoma cells in a concentration dependent manner.
Wang
Liang
L
College of Life Science, Liaoning Normal University, Dalian, China.
Liu
Cuiping
C
Jia
Dan
D
Zou
Wei
W
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects*; D008099:Liver / Q000737:chemistry / Q000187:drug effects*; D010713:Phosphatidylcholines / Q000302:isolation & purification / Q000494:pharmacology*; D034421:Sus scrofa; D013552:Swine
D010713:Phosphatidylcholines