Dielectrophoresis based-cell patterning for tissue engineering.
Engineering functional tissues and organs in vitro is considered integral to regenerative medicine. Many recent cell patterning technique developments position cells at a pre-designated pattern to improve tissue engineering efficiency and quality and to facilitate 3-D cell-cell interaction exploration. Among these techniques, dielectrophoresis (DEP)-based cell patterning advantageously offers speed, ease of operation, low degree of cell damage, and precision. This article reviews recent advances in DEP-based patterning techniques, including electrode design, suitable buffer and hydrogel, effects of the electric current to cells, combination potential with other techniques, as well as efforts to generate 3-D tissues.
Lin
Ruei-Zeng
RZ
Institute of Molecular Medicine, National Tsing Hua University, Hsin Chu, Taiwan, ROC.
Ho
Chen-Ta
CT
Liu
Cheng-Hsien
CH
Chang
Hwan-You
HY
D000818:Animals; D001698:Biomedical Engineering / Q000379:methods; D001709:Biotechnology / Q000379:methods* / Q000639:trends; D002021:Buffers; D018929:Cell Culture Techniques / Q000379:methods; D004566:Electrodes; D004586:Electrophoresis / Q000379:methods*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*
D002021:Buffers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate