title: Identification of genes differentially expressed during ripening of banana.
abstract: The banana (Musa acuminata, subgroup Cavendish 'Grand Nain') is a climacteric fruit of economic importance. A better understanding of the banana ripening process is needed to improve fruit quality and to extend shelf life. Eighty-four up-regulated unigenes were identified by differential screening of a banana fruit cDNA subtraction library at a late ripening stage. The ripening stages in this study were defined according to the peel color index (PCI). Unigene sequences were analyzed with different databases to assign a putative identification. The expression patterns of 36 transcripts confirmed as positive by differential screening were analyzed comparing the PCI 1, PCI 5 and PCI 7 ripening stages. Expression profiles were obtained for unigenes annotated as orcinol O-methyltransferase, putative alcohol dehydrogenase, ubiquitin-protein ligase, chorismate mutase and two unigenes with non-significant matches with any reported sequence. Similar expression profiles were observed in banana pulp and peel. Our results show differential expression of a group of genes involved in processes associated with fruit ripening, such as stress, detoxification, cytoskeleton and biosynthesis of volatile compounds. Some of the identified genes had not been characterized in banana fruit. Besides providing an overview of gene expression programs and metabolic pathways at late stages of banana fruit ripening, this study contributes to increasing the information available on banana fruit ESTs.
authors.affiliation: Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato. Km 9.6 Libramiento Norte Carretera Irapuato-León, Guanajuato, México.
authors.forename: Sandra Mabel
authors.initials: SM
authors.lastname: Manrique-Trujillo
authors.forename: Ana Cecilia
authors.initials: AC
authors.lastname: Ramírez-López
authors.forename: Enrique
authors.initials: E
authors.lastname: Ibarra-Laclette
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Gómez-Lim
mesh_terms: D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005638:Fruit / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D028521:Musa / Q000235:genetics* / Q000254:growth & development; D009693:Nucleic Acid Hybridization
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant