One ring to rule them all? Another cellular responsibility for PCNA.
To prevent duplication or loss of genomic regions during DNA replication, it is essential that the entire genome is copied precisely once every S phase. Cells achieve this by mutually exclusive regulation of origin firing and licensing. A crucial protein that is involved in origin licensing is chromatin licensing and DNA replication factor 1 (CDT1) and, therefore, activity of this protein must be strictly controlled. Four recent articles have demonstrated that proliferating cell nuclear antigen (PCNA), an essential sliding clamp used in replication and DNA repair, has a crucial role in this process by mediating the proteasomal degradation of CDT1.
Green
Catherine M
CM
Genome Damage and Stability Centre, University of Sussex, Brighton, BN19RQ, UK. c.m.green@sussex.ac.uk
D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000378:metabolism / Q000502:physiology*; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D018809:Proliferating Cell Nuclear Antigen / Q000502:physiology*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D029867:Xenopus Proteins / Q000378:metabolism / Q000502:physiology*
C406819:Cdt1 protein, Xenopus; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D018809:Proliferating Cell Nuclear Antigen; D029867:Xenopus Proteins; D046988:Proteasome Endopeptidase Complex