Force development at elevated [Mg2+]o and [K+]o in myocardium from the freshwater turtle (Trachemys scripta) and influence of factors associated with hibernation.
The effects of high [Mg(2+)](o) on force development were examined for heart muscle of freshwater turtle. Plasma [Mg(2+)] during hibernation may increase drastically and like plasma [K(+)] approach values as high as 10 mM. Each experiment performed at either 20 or 5 degrees C involved four ventricular preparations of which one pair was exposed to 10, and one to 1 mMMg(2+). One preparation of each pair was furthermore exposed to 10 mM K(+), whereas the other was maintained at 2.5 mM K(+). During oxygenation, high relative to low [Mg(2+)](o) displayed a weak tendency to reduce twitch force; a tendency that was not reduced by elevations of [Ca(2+)](o). Severe hypoxia accentuated the negative effect of high [Mg(2+)](o). This effect disappeared, however, when hypoxia was combined with acidosis obtained by 24 mM lactic acid. In comparison to [Mg(2+)](o), high [K(+)](o) strongly depressed force development under both oxygenation and hypoxia, but no consistent interplay between the two ions was revealed. The negative inotropic effects of both high [Mg(2+)](o) and high [K(+)](o) were reduced or eliminated by 10 muM adrenaline. In conclusion the cardiac effects of elevations in [Mg(2+)](o) appear to be small during hibernation, in particular when considering the concomitant adrenergic stimulation and acidosis.
Gesser
H
H
Institute of Biological Sciences, University of Aarhus, Building 1131, DK-8000 Aarhus C, Denmark. hans.gesser@biology.au.dk
D000138:Acidosis / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006605:Hibernation / Q000502:physiology*; D000860:Hypoxia / Q000378:metabolism; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology*; D009200:Myocardial Contraction / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology*; D013696:Temperature; D014426:Turtles / Q000502:physiology*
D019344:Lactic Acid; D008274:Magnesium; D011188:Potassium; D002118:Calcium; D004837:Epinephrine