title: Microbiological assay for lomefloxacin in coated tablets.
abstract: The validation of a microbiological assay, applying cylinder plate method for determination of the activity of lomefloxacin in coated tablets is described. Using a strain of Bacillus subtilis ATCC 9372 as the test organism, lomefloxacin was measured in concentrations ranging from 2.0 to 8.0 microg/mL. The method validation showed that it is linear (r = 0.9999), precise (relative standard deviation = 1.15%), and accurate (it measured the added quantities). The excipients did not interfere in the determination. It was concluded that the microbiological assay is satisfactory for quantitation of lomeflox tablets.
authors.affiliation: Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, UNESP, Rod. Araraquara-Jaú, km 1, CEP 14801-902, Araraquara, SP, Brazil.
authors.forename: Greici Cristiani
authors.initials: GC
authors.lastname: Gomes
authors.forename: Hérida Regina Nunes
authors.initials: HR
authors.lastname: Salgado
mesh_terms: D000995:Antitubercular Agents / Q000032:analysis*; D001412:Bacillus subtilis / Q000378:metabolism; D001681:Biological Assay; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004058:Diffusion; D024841:Fluoroquinolones / Q000032:analysis*; D008826:Microbial Sensitivity Tests; D008828:Microbiological Techniques / Q000379:methods*; D008956:Models, Chemical; D015363:Quinolones / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets
chemical_list: D000995:Antitubercular Agents; D024841:Fluoroquinolones; D015363:Quinolones; D013607:Tablets; C053091:lomefloxacin