Chronic hypoxia modulates endothelium-dependent vasorelaxation through multiple independent mechanisms in ovine cranial arteries.
Acclimatization to chronic hypoxia involves numerous compensatory changes in many tissues, including blood vessels. The present data demonstrate that in addition to well-documented changes in contractility, chronic hypoxia also produces important changes in the mechanisms mediating endothelium-dependent vasodilatation. At the level of the endothelium, hypoxia attenuates endothelial release of NO and this appears to be mediated through reductions in eNOS specific activity; chronic hypoxia has little effect on eNOS abundance. In contrast, chronic hypoxia depresses the abundance of sGC, which functions as the downstream vascular receptor for NO released from the endothelium. The decreased abundance of sGC produced by chronic hypoxia occurs without changes in sGC specific activity and results in decreased rates of NO-induced cGMP synthesis. Nonetheless, the vasodilator efficacy of NO is enhanced in hypoxic arteries, which suggests that mechanisms downstream from sGC are upregulated by hypoxia. Consistent with this view, chronic hypoxia significantly depresses PDE activity, which serves to prolong cGMP half-life and enhance its vasodilator effects. It remains possible that chronic hypoxia may also enhance PKG activity and/or the abundance of its substrates; this possibility remains a promising topic for future investigation. Overall, it is important to recognize that the mechanisms of adaptation to chronic hypoxia identified in the present study may be somewhat unique to adult carotid arteries. Adaptive responses to chronic hypoxia can vary considerably between small and large arteries, and also between immature and adult arteries . Still, the present data clearly demonstrate that both the endothelium and vascular smooth muscle of major arteries are profoundly influenced by chronic hypoxia, and thereby participate fully in whole-body adaptation to reduced oxygen availability.
Pearce
William J
WJ
Center for Perinatal Biology, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA. wpearce@som.llu.edu
Williams
James M
JM
Hamade
Mohammad W
MW
Chang
Melody M
MM
White
Charles R
CR
D000818:Animals; D002536:Cerebral Arteries / Q000378:metabolism* / Q000503:physiopathology; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000503:physiopathology; D000860:Hypoxia / Q000378:metabolism* / Q000503:physiopathology; D012756:Sheep; D014664:Vasodilation / Q000502:physiology*