Express method for detection of Amanita phalloides amanitine toxins.
Spectral studies of the interaction between amanitine and ethidium bromide fluorophore showed the appearance of a new intensive fluorescence band after addition of amanitine to ethidium bromide solution, caused by the formation of a charge-transfer complex. The new fluorescence band is located in a shorter wave region of the spectrum compared to ethidium bromide fluorescence band. Based on the results, a rapid fluorescent method for detection of amanitines was developed.
Vlaskin
D N
DN
Military University of Radiation, Chemical, and Biological Defense, Moscow.
Gainullina
E T
ET
Klyuster
O V
OV
Rybal'chenko
I V
IV
Ryzhikov
S B
SB
Taranchenko
V F
VF
D000545:Amanita / Q000378:metabolism*; D000546:Amanitins / Q000032:analysis*; D004996:Ethidium / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000546:Amanitins; C049197:beta-amanitin; D004996:Ethidium