title: Characterization of enantioselective binding of racemic natural tetrahydropalmatine to DNA by chromatographic methods.
abstract: A racemate from natural product, tetrahydropalmatine (THP), was characterized on its enantioselective binding to DNA by the chromatographic methods including microdialysis/HPLC, centrifugal ultrifiltration/HPLC and immobilized DNA affinity chromatography. It was found that its (+)-enantiomer was preferential to binding on B-form duplex DNA including calf thymus DNA, AT and GC sequence oligo DNA, as well as triplex oligo DNA. The binding constants of the THP enantiomers to ct-DNA were determined with the methods of microdialysis/HPLC and frontal affinity chromatography. In addition, the DNA structural preference of either enantiomer was evaluated with the chromatographic methods.
authors.affiliation: National Chromatographic R. & A. Centre, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Xingye
authors.initials: X
authors.lastname: Su
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
authors.forename: Liang
authors.initials: L
authors.lastname: Kong
authors.forename: Junjie
authors.initials: J
authors.lastname: Ou
authors.forename: Chuanhui
authors.initials: C
authors.lastname: Xie
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D001600:Berberine Alkaloids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D017551:Microdialysis; D013237:Stereoisomerism; D014461:Ultracentrifugation
chemical_list: D001600:Berberine Alkaloids; C014215:tetrahydropalmatine; D004247:DNA