title: Kinetic flux profiling of nitrogen assimilation in Escherichia coli.
abstract: We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.
authors.affiliation: Department of Chemistry and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Yuan
authors.forename: William U
authors.initials: WU
authors.lastname: Fowler
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Kimball
authors.forename: Wenyun
authors.initials: W
authors.lastname: Lu
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Rabinowitz
mesh_terms: D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D009584:Nitrogen / Q000378:metabolism*; D013997:Time Factors
chemical_list: D009584:Nitrogen