A unique monoclonal antibody against human plasma fibronectin, which recognizes an epitope on the surface of a subpopulation of polymorphonuclear neutrophils.
A new monoclonal antibody (MAb) specific to plasma fibronectin, named MO, which was produced by immunizing a mouse with fragments of fibronectin isolated from human plasma, was found to bind to the surface of a subpopulation of peripheral blood polymorphonuclear neutrophils (PMNs) in the presence of physiological concentration of soluble fibronectin. MAb MO had no reactivity with other blood cells such as lymphocytes, monocytes, or platelets. The percentage of the MAb MO-reactive subpopulation of PMNs from healthy donors varied from 0 to 60%. Ten previously established MAbs against fibronectin tested in this study together with MAb MO had no reactivity with the surface of PMNs at all, when tested simultaneously with MAb MO. These results indicate that MAb MO can be a unique and useful tool for analyzing the structure and function of fibronectin-especially the fibronectin expressed on the surface of PMNs.
Okada
Mariko
M
Department of Medical Technology, Ehime Prefectural University of Health Sciences, Ehime, Japan. mokada@epu.ac.jp
Hitsumoto
Yasuo
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000918:Antibody Specificity / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D005260:Female; D005353:Fibronectins / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005353:Fibronectins