Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells.
The interactions between granulocyte colony-stimulating factor (G-CSF) and dextran sulfate (DS) with different chain lengths and sulfate contents were studied by capillary zone electrophoresis. It was found that DS with a molecular mass of 500 kDa (DS500) could bind to G-CSF and the binding constant and binding sites were determined using Scatchard plot to be 1.17 x 10(6) M(-1) and 3, respectively. DS with a molecular mass of 40 kDa also had the affinity to G-CSF and the binding constant and binding sites were 1.01 x 10(6) M(-1) and 8, respectively. However, DS with a molecular mass of 8 kDa and the non-sulfated saccharide, dextran, had no affinity to G-CSF. The results indicate that the interactions between G-CSF and DS are dependent on the chain lengths and sulfate contents of the saccharides. In addition, the effects of G-CSF-binding DS on a G-CSF-dependent leukemia cell line were investigated using biological methods. Results show that DS500 plus G-CSF has potential therapeutic effect on cancers because their combination could inhibit the growth and induce the differentiation of the leukemia cells.
Liang
Aiye
A
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Zhou
Xiaomian
X
Wang
Qiuyan
Q
Liu
Xin
X
Qin
Jianhua
J
Du
Yuguang
Y
Wang
Keyi
K
Lin
Bingcheng
B
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D016264:Dextran Sulfate / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D019075:Electrophoresis, Capillary / Q000379:methods*; D016179:Granulocyte Colony-Stimulating Factor / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D007938:Leukemia / Q000188:drug therapy; D051379:Mice; D008970:Molecular Weight; D011485:Protein Binding; D014407:Tumor Cells, Cultured
D016179:Granulocyte Colony-Stimulating Factor; D016264:Dextran Sulfate