title: Endothelial nitric oxide synthase gene intron 4 a/b VNTR polymorphism in children with APSGN.
abstract: The role of endothelial nitric oxide synthase gene intron 4 a/b (eNOS4a/b) variable number of tandem repeats (VNTR) polymorphism in various renal diseases was investigated. We investigated whether the eNOS4a/b VNTR polymorphism is associated with susceptibility to acute poststreptococcal glomerulonephritis (APSGN) and its clinical features. Endothelial NOS4a/b VNTR polymorphism is determined by the polymerase chain reaction in 60 children with APSGN, and 66 healthy controls. The genotype distribution of eNOS4 does not differ between the patients and the controls (X(2)=5.1, p=0.079). However, the frequency of eNOS4a (eNOS4a/a and eNOS4a/b) genotype is higher in the patients than in the controls (X(2)=4.5, p=0.046). In the APSGN group we performed renal biopsy on eight patients because of nephrotic syndrome accompanies acute nephritic syndrome or glomerular filtration rate (GFR) is lower than 50% of normal, and found that to carry a/a and a/b genotypes were a significant risk factor for this type presentation (OR=17.3, 95% CI:1.95-152.67, p=0.03). Mean serum creatinine values are found statistically significantly higher in a/a and a/b genotypes when compared with b/b genotypes (p=0.022). Children carrying the "aa" and "ab" genotype or "a" allele of eNOS4 have a greater tendency to develop and clinical presentation of APSGN.
authors.affiliation: Pediatric Nephrology, Cukurova University, Balcali Adana, Yuregir, 01330, Turkey. dursunhs@yahoo.com
authors.forename: Hasan
authors.initials: H
authors.lastname: Dursun
authors.forename: Aytul
authors.initials: A
authors.lastname: Noyan
authors.forename: Selcuk
authors.initials: S
authors.lastname: Matyar
authors.forename: Mithat
authors.initials: M
authors.lastname: Buyukcelik
authors.forename: Mustafa
authors.initials: M
authors.lastname: Soran
authors.forename: Nurcan
authors.initials: N
authors.lastname: Cengiz
authors.forename: Gulsah
authors.initials: G
authors.lastname: Seydaoglu
authors.forename: Gulen
authors.initials: G
authors.lastname: Attila
authors.forename: Aysun K
authors.initials: AK
authors.lastname: Bayazit
authors.forename: Ali
authors.initials: A
authors.lastname: Anarat
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005921:Glomerulonephritis / Q000235:genetics* / Q000382:microbiology; D006801:Humans; D007438:Introns; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013290:Streptococcal Infections / Q000235:genetics*; D020080:Tandem Repeat Sequences
chemical_list: D052250:Nitric Oxide Synthase Type III