Release of ferulic acid and feruloylated oligosaccharides from sugar beet pulp by Streptomyces tendae.
Given several promising industrial applications of ferulic acid, this study was designed to identify actinomycete strains able to release high levels of this acid from sugar beet pulp (SBP). Out of 47 strains tested, 37% were found to release free ferulic acid from the growth substrate. One strain, identified as Streptomyces tendae by 16S RNA gene sequencing, was capable of releasing 80% of the ferulic acid ester-linked to the pectin in SBP after 5 days of growth. These data suggest that some actinomycetes are able to release ferulic acid and feruloylated oligosaccharides from SBP. During growth on SBP, it seems that Streptomyces species solubilize and release feruloylated oligosaccharides by specific carbohydrase activities before de-esterification and release of free ferulic acid.
Ferreira
P
P
Departamento de Microbiología y Parasitología, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
Diez
N
N
Faulds
C B
CB
Soliveri
J
J
Copa-Patiño
J L
JL
D000383:Agriculture; D027461:Beta vulgaris / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
D003373:Coumaric Acids; D009844:Oligosaccharides; D012336:RNA, Ribosomal, 16S; C004999:ferulic acid