title: Lack of DNA damage induced by fluoride on mouse lymphoma and human fibroblast cells by single cell gel (comet) assay.
abstract: Fluoride has widely been used in Dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury on genetic apparatus. Genotoxicity tests constitute an important part of cancer research for risk assessment of potential carcinogens. In this study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Mouse lymphoma and human fibroblast cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 microg/mL for 3 h at 37 degrees C. The results pointed out that NaF in all tested concentrations did not contribute to DNA damage as depicted by the mean tail moment and tail intensity for both cellular types assessed. These findings are clinically important because they represent a valuable contribution for evaluation of the potential health risk associated with exposure to agents usually used in dental practice.
authors.affiliation: Department of Pathology, Center for Genotoxin (TOXICAN), Faculty of Medicine of Botocatu, State University of São Paulo, Botucatu, SP, Brazil. ak92@hotmail.com
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
authors.forename: Patrícia Lepage
authors.initials: PL
authors.lastname: Alves de Lima
authors.forename: Mariângela Esther Alencar
authors.initials: ME
authors.lastname: Marques
authors.forename: Daisy Maria Fávero
authors.initials: DM
authors.lastname: Salvadori
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002327:Cariostatic Agents / Q000009:adverse effects*; D020552:Comet Assay; D004249:DNA Damage; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008223:Lymphoma / Q000235:genetics / Q000473:pathology; D051379:Mice; D012969:Sodium Fluoride / Q000009:adverse effects*
chemical_list: D002327:Cariostatic Agents; D012969:Sodium Fluoride