title: Genetic structure and transcriptional analysis of the arginine deiminase (ADI) cluster in Lactococcus lactis MG1363.
abstract: In a recent proteomic analysis, we showed the overproduction of the ArcA and ArcB proteins in Lactococcus lactis MG1363 at low pH. The corresponding genes belong to the arcABD1C1C2TD2 cluster that encodes components of the arginine deiminase pathway. In this study, we characterized this cluster at the genetic level. Northern blot experiments showed the expression of at least seven transcripts, all induced by acidity. Transcript analysis using 5'RACE PCR (rapid amplification of cDNA ends polymerase chain reaction) in the arcB-arcD1 intergenic region. In silico analysis identified nine stem-loop structures, all located in intergenic regions. Collectively, these data suggest a role for RNA processing and (or) premature termination in the differential expression of genes within the arcABD1C1C2TD2 cluster.
authors.affiliation: Laboratoire de microbiologie de l'environment, USC Institut national de la recherche agronomique (INRA), Université de Caen, Cedex, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Budin-Verneuil
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Maguin
authors.forename: Yanick
authors.initials: Y
authors.lastname: Auffray
authors.forename: Dusko S
authors.initials: DS
authors.lastname: Ehrlich
authors.forename: Vianney
authors.initials: V
authors.lastname: Pichereau
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000235:genetics* / Q000378:metabolism; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics*; D005810:Multigene Family; D009876:Operon / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D012335:RNA, Ribosomal; D006867:Hydrolases; C019723:arginine deiminase