title: The chloroplast lumen and stromal proteomes of Arabidopsis thaliana show differential sensitivity to short- and long-term exposure to low temperature.
abstract: Cold acclimation and over-wintering by herbaceous plants are energetically expensive and are dependent on functional plastid metabolism. To understand how the stroma and the lumen proteomes adapt to low temperatures, we have taken a proteomic approach (difference gel electrophoresis) to identify proteins that changed in abundance in Arabidopsis chloroplasts during cold shock (1 day), and short- (10 days) and long-term (40 days) acclimation to 5 degrees C. We show that cold shock (1 day) results in minimal change in the plastid proteomes, while short-term (10 days) acclimation results in major changes in the stromal but few changes in the lumen proteome. Long-term acclimation (40 days) results in modulation of the proteomes of both compartments, with new proteins appearing in the lumen and further modulations in protein abundance occurring in the stroma. We identify 43 differentially displayed proteins that participate in photosynthesis, other plastid metabolic functions, hormone biosynthesis and stress sensing and signal transduction. These findings not only provide new insights into the cold response and acclimation of Arabidopsis, but also demonstrate the importance of studying changes in protein abundance within the relevant cellular compartment.
authors.affiliation: Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-901 87 Umeå, Sweden.
authors.forename: Estelle
authors.initials: E
authors.lastname: Goulas
authors.forename: Maria
authors.initials: M
authors.lastname: Schubert
authors.forename: Thomas
authors.initials: T
authors.lastname: Kieselbach
authors.forename: Leszek A
authors.initials: LA
authors.lastname: Kleczkowski
authors.forename: Per
authors.initials: P
authors.lastname: Gardeström
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schröder
authors.forename: Vaughan
authors.initials: V
authors.lastname: Hurry
mesh_terms: D000064:Acclimatization; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism* / Q000502:physiology; D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D010788:Photosynthesis; D040901:Proteomics; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins