The use of micronucleus test for detection of genotoxic damage to the thyroid gland.
We studied the possibility of using the micronucleus test in in vivo experiments on the model of rat follicular thyrocytes prestimulated to cell division (hemithyroidectomy). Single administration of N-nitroso-N-methylurea produced a significant dose-dependent effect on micronucleus formation in thyrocytes and polychromatophilic erythrocytes of the bone marrow. The test system allowed us to reveal a cumulative effect of 2-fold and 4-fold treatment with the mitogen in low or subthreshold doses on the thyroid gland. Our results indicate that the micronucleus test is an informative method for the analysis of the effect of genotoxic agents on the thyroid parenchyma.
Pavlov
A V
AV
Department of Histology, Cytology, and Embryology, Yaroslavl State Medical Academy. pavlov@hist.yma.ac.ru
Gansburgskii
M A
MA
Gansburgskii
A N
AN
Shashkina
M V
MV
Miro
T L
TL
D000818:Animals; D004249:DNA Damage; D008297:Male; D008770:Methylnitrosourea / Q000633:toxicity; D015162:Micronucleus Tests; D008934:Mitogens / Q000633:toxicity*; D051381:Rats; D013961:Thyroid Gland / Q000187:drug effects* / Q000473:pathology
D008934:Mitogens; D008770:Methylnitrosourea