[The comparison of mycoplasma detection methods in the urogenital tract infection].
Six different methods have been employed to detect M. hominis (Mh) and U. urealyticum (Uu) in clinical samples collected from 67 men. The results obtained by PCR and IF test were approximately equal: 13.6 and 13.44%--Mh and 44.4 and 48.8%--Uu, respectively. Mycoplasmas were detected by cultural method less frequently (9.6%--Mh, 32.2%--Uu). The highest infection rates were obtained in the test for blood antigens (40%--Mh and 63%--Uu). At present a commercial diagnosticum to detect mycoplasma antigents in blood is lacking. Sometimes the results of cultural method are positive, while the PCR results are negative. So the optimal scheme based on both PCR and culture has been proposed.
Balabanov
D N
DN
Rakovskaia
I V
IV
Gorina
L G
LG
Goncharova
S A
SA
Gamova
N A
NA
Barkhatova
O I
OI
D000208:Acute Disease; D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis / Q000097:blood; D019085:Fluorescent Antibody Technique, Direct; D016680:Genome, Bacterial / Q000235:genetics; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases / Q000175:diagnosis* / Q000382:microbiology; D008875:Middle Aged; D009175:Mycoplasma Infections / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D019535:Mycoplasma hominis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D017410:Practice Guidelines as Topic; D012680:Sensitivity and Specificity; D016869:Ureaplasma Infections / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D016990:Ureaplasma urealyticum / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014566:Urogenital System / Q000276:immunology / Q000382:microbiology
D000942:Antigens, Bacterial