title: [Determination of orlistat in health food for controlling body weight by high performance liquid chromatography/electrospray spectrometry].
abstract: OBJECTIVE
  To establish a method of determining orlistat in health food by on line HPLC-UV-ESI/MS.
  
  
  METHODS
  The separation was completed on an analytical Spherigel C8 column (5 microm, 200 mm x 4.6 mm) with acetonitrile (0.1% formic acid) : water (0.1% formic acid) = 80 : 20 as mobile phase, the detection wavelength was 2003 mm.
  
  
  RESULTS
  Good linearity between peak areas and concentrations was obtained during rag of 0.3 - 0.6 mg/ml.
  
  
  CONCLUSIONS
  The method is simple and can be used to determine orlistat in the health food for controlling body weight accurately.
authors.affiliation: Analytical and Testing Center of Human Normal University, Changsha 410081, China.
authors.forename: Song
authors.initials: S
authors.lastname: Xiao
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Zhu
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Shou-Zhuo
authors.initials: SZ
authors.lastname: Yao
mesh_terms: D019440:Anti-Obesity Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D006801:Humans; D007783:Lactones / Q000032:analysis*; D000077403:Orlistat; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D019440:Anti-Obesity Agents; D007783:Lactones; D000077403:Orlistat