[Expression of GRO-1 and its relationship with VEGF in squamous cell carcinoma of larynx].
OBJECTIVE
To investigate the correlation between expression of growth regulated oncogene 1 (GRO-1) and some clinical biological characteristics of laryngeal carcinoma.


METHODS
Expression of GRO-1 and VEGF(vascular endothelial growth factor) protein were investigated by means of immunohistochemistry in 60 laryngeal carcinoma tissues, GRO-1 was detected by RT-PCR in 30 laryngeal carcinoma tissues and 10 normal laryngeal tissues.


RESULTS
The result of RT-PCR showed that the expression of GRO-1 in squamous cell carcinoma of larynx tissues was higher than those in normal laryngeal tissues (P < 0.01). The result of Immunohistochemistry revealed that the expression of GRO-1 in squamous cell carcinoma of larynx with the metastasis of lymph nodes was higher than those without metastasis (P < 0.05). There was no significant relationship between GRO-1 protein expression and patients'tumor histological difference (P > 0.05). The expression of GRO-1 had no significant difference in age, sex, location and T classification (P > 0.05). There was positive correlation in staining intensity between GRO-1 and VEGF (P < 0.05).


CONCLUSIONS
GRO-1 can promote tumor growth, metastasis by several mechanism,the effect of GRO-1 on angiogenesis are consistent with the distribution of VEGF.
Liu
Shi
S
Department of Otorhinolaryngology-Head and Neck Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China. yushifirst@163.com
Wang
Xin
X
Xu
Xiuyu
X
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D054360:Chemokine CXCL1 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A