[Impact of rhG-CSF on sphingosine 1-phosphate concentration in blood plasma of donors].
Sphingosine 1-phosphate (S1P), which can be impacted by different growth factors through sphingosine kinase (SphK), is a bioactive lipid produced by metabolism of sphingolipid with various biological responses. Recombinant human granulocyte-colony-stimulating factor (rhG-CSF) have been used widely in peripheral blood stem/progenitor cell mobilization. This study was aimed to investigate the effects of rhG-CSF on S1P concentration in plasma of donors. The peripheral blood mononuclear cells and blood plasma were collected from 8 peripheral blood progenitor cell donors before mobilization and on the fifth day of mobilization with rhG-CSF. The SphK mRNA expression of blood mononuclear cells were detected by RT-PCR. The changes of S1P concentration were assayed with SphK enzyme catalyzed reaction. The results showed that both kinds blood mononuclear cells collected before and after rhG-CSF mobilization expressed SphK mRNA. The S1P concentration is low in donor's plasma both before and after mobilization with rhG-CSF, and there was no markedly change of S1P concentration in plasma before and after mobilization (P > 0.05). In conclusion, mobilization with rhG-CSF does not impact S1P concentration in donors' plasma.
Huang
Wen-Rong
WR
Department of Hematology, General Hospital of Chinese PLA, Beijing 100853, China.
Wang
Li-Sheng
LS
Duan
Hai-Feng
HF
Gao
Chun-Ji
CJ
Lu
Zhuo-Zhuang
ZZ
Wang
Hua
H
Da
Wan-Ming
WM
D001782:Blood Donors; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D008246:Lysophospholipids / Q000097:blood*; D012333:RNA, Messenger / Q000097:blood; D011994:Recombinant Proteins; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000097:blood
D008246:Lysophospholipids; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; C060506:sphingosine 1-phosphate; D013110:Sphingosine