[Effect of cytarabine on expression of cell adhesion molecules and on endothelium-leukocyte interaction in vitro].
OBJECTIVE
To characterize cytarabine effects on human endothelium expression of cell adhesion molecules (CAM) of various classes and interaction of cultured endothelial cells (EC) with blood leukocytes.


METHODS
Primary cultures of EC of human umbilical vein were obtained by EC culturing for 24-72 h with cytarabine (10 mcg/ml). They were investigated with phase-contrast microscopy, immunohistochemistry and flow cytometry.


RESULTS
Cytarabine effects manifested with EC-induced expression of cell surface P- and E-selectines and VCAM-1; ICAM-1 expression rapidly enhanced, leukocyte adhesion to endothelium also activates.


CONCLUSIONS
Cytarabine, as a pathogenetic factor, induces proinflammatory alterations of the endothelium responsible for development of vascular complications in patients on cytostatic therapy.
Chervontseva
A M
AM
Romanov
Iu A
IuA
Savchenko
V G
VG
D000964:Antimetabolites, Antineoplastic / Q000009:adverse effects*; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002478:Cells, Cultured; D003561:Cytarabine / Q000009:adverse effects*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000166:cytology*
D000964:Antimetabolites, Antineoplastic; D015815:Cell Adhesion Molecules; D003561:Cytarabine