Controlled release of DNA/polyamine complex by photoirradiation of a solid phase presenting o-nitrobenzyl ether tethered spermine or polyethyleneimine.
Various gene transfer and automated/monitorized analytical applications require the controlled release of nucleic acid. A solid phase with spermine or polyethyleneimines (PEI, 600 MW) tethered by o-nitrobenzyl linkages was synthesized with polyethylene oxide beads (ArgoGel-NH(2)). The photolysis of test compound O-2-nitrophenethyl O,O-diethyl phosphate or solid phase with o-nitrobenzyl group as synthetic linker was completely degradable with photoirradiation at 365 nm for 10-18 min at 3.5 mW/cm(2). DNA binding with polyamine of the solid phase and releasing of DNA/polyamine were monitored by UV measurement and gel electrophoresis. The potential exists to employ a DNA-loaded solid phase for spatially, temporally, or dose-controlled release of DNA, at extracellular or intracellular sites.
Kim
Moon Suk
MS
Institute for Medicine and Engineering, Department of Chemical and Biomolecular Engineering, 1024 Vagelos Research Laboratory, University of Pennsylvania, Philadelphia, PA 19104, USA.
Diamond
Scott L
SL
D004247:DNA / Q000008:administration & dosage / Q000378:metabolism*; D016503:Drug Delivery Systems; D004987:Ethers; D006801:Humans; D008027:Light; D011073:Polyamines / Q000008:administration & dosage / Q000378:metabolism*; D011094:Polyethyleneimine; D013096:Spermine
D004987:Ethers; D011073:Polyamines; D013096:Spermine; D011094:Polyethyleneimine; D004247:DNA