Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections.
OBJECTIVE
The kinetics of three serological markers (IgM, IgA, and IgG) in serum, saliva, and urine samples from adult patients with primary or secondary dengue infection were studied.


METHODS
Serum, saliva, and urine samples were collected from 22 patients with clinical and confirmed dengue 3 virus infection during the outbreak in Havana City in 2001. They were tested by capture IgM (MAC-ELISA), IgA (AAC-ELISA), and IgE (EAC-ELISA) and IgG ELISA inhibition method (EIM) to detect specific dengue antibodies.


RESULTS
Similar kinetics were observed in IgM, IgA, and IgG antibodies in saliva and IgA and IgG in urine samples from secondary cases compared with kinetics in serum samples, although the values were lower. No IgG antibody was detected in saliva and urine samples in primary cases and IgM antibody was not detected in urine samples from either primary or secondary infection. All secondary cases were positive for IgG in saliva and urine samples at day 7. The kinetics of specific IgE antibodies in primary and secondary cases were different.


CONCLUSIONS
The kinetics of three serological markers (IgM, IgA, and IgG) in serum, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infection were studied for the first time, showing its behavior and usefulness in dengue virus diagnosis. The specific IgE could play a role as a serological marker in secondary infections.
Vázquez
S
S
"Pedro Kourí" Tropical Medicine Institute, PAHO/WHO Collaborating Center for Dengue and its Vectors, Autopista Novia del Mediodía, Km 6 1/2, La Lisa, Havana City, Cuba. svazquez@ipk.sld.cu
Cabezas
S
S
Pérez
A B
AB
Pupo
M
M
Ruiz
D
D
Calzada
N
N
Bernardo
L
L
Castro
O
O
González
D
D
Serrano
T
T
Sanchez
A
A
Guzmán
M G
MG
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood / Q000652:urine; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis / Q000097:blood / Q000652:urine; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood / Q000652:urine; D007075:Immunoglobulin M / Q000032:analysis / Q000097:blood / Q000652:urine; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007073:Immunoglobulin E