Randomly amplified polymorphic DNA as a tool for genotoxicity: an assessment.
Novel short-term assays are required to substantiate the battery of assessment methods for evaluating the genotoxicity of candidate drugs. In this study, an attempt has been made to evaluate randomly amplified polymorphic DNA (RAPD) analysis for its potential to establish genotoxic effect of a known genotoxicant, ie, ethyl methanesulfonate (EMS) in Swiss mice (Mus musculus). Based on the RAPD profiles, genetic damages were detected in EMS-exposed animals, suggesting its usefulness in scanning whole genome for assessing the genotoxic effects of candidate drugs. The profiles were generated using genomic DNA, isolated from liver prior to treatment and from liver, bone marrow and blood after treatment of the genotoxicant. Measurable differences indicative of genetic damages were observed when the pre- and post-treatment profiles were compared. This suggests that RAPD analysis may be useful for assessing the pre-clinical genotoxic effects of candidate drugs.
Noel
Sanjeev
S
Division of Toxicology, Central Drug Research Institute, MG Marg, Lucknow India.
Rath
Srikanta Kumar
SK
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D004247:DNA / Q000097:blood / Q000187:drug effects; D004249:DNA Damage; D016172:DNA Fingerprinting; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D005069:Evaluation Studies as Topic; D008099:Liver / Q000187:drug effects; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D013997:Time Factors
D009153:Mutagens; D004247:DNA; D005020:Ethyl Methanesulfonate