Role of T and NK cells and IL7/IL7r interactions during neonatal maturation of lymph nodes.
Lymph node (LN) development depends on prenatal interactions occurring between LN inducer and LN organizer cells. We have distinguished defects in LN formation due to failure in embryonic development (aly/aly) from defects in postnatal maturation (Il2rgamma(-/-)Rag2(-/-)). Both mutant strains form normal primordial LNs with differing fate. In aly/aly mice, the LN primordium dissipates irreversibly late in gestation; in contrast, Il2rgamma(-/-)Rag2(-/-) LN anlage persists for a week after birth but disperses subsequently, a process reversible by neonatal transfer of WT IL7r(+) TCR(+) T or natural killer (NK) cells, suggesting a role for IL7/IL7r interactions. Thus, we reveal a unique stage of postnatal LN development during which mature lymphocytes and IL7/IL7r interactions may play an important role.
Coles
Mark C
MC
Division of Molecular Immunology and Immune Cell Biology and Confocal Microscopy and Image Analysis Laboratory, National Institute for Medical Research, Mill Hill, London, United Kingdom. m542@york.ac.uk
Veiga-Fernandes
Henrique
H
Foster
Katie E
KE
Norton
Trisha
T
Pagakis
Stamatis N
SN
Seddon
Ben
B
Kioussis
Dimitris
D
D019264:Adoptive Transfer; D000818:Animals; D000831:Animals, Newborn; D018076:DNA, Complementary; D049452:Green Fluorescent Proteins / Q000378:metabolism; D015851:Interleukin-7 / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008198:Lymph Nodes / Q000196:embryology / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D008954:Models, Biological; D009154:Mutation; D016328:NF-kappa B / Q000235:genetics; D020395:Receptors, Interleukin-7 / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D019076:Transgenes
D018076:DNA, Complementary; D015851:Interleukin-7; D016328:NF-kappa B; D020395:Receptors, Interleukin-7; D049452:Green Fluorescent Proteins