Liquid chromatographic determination of deoxynivalenol in baby food and animal feed: interlaboratory study.
An interlaboratory study was conducted for the determination of deoxynivalenol in baby food and animal feed by high-performance liquid chromatography with UV detection. The study included 14 participants representing a cross section of industry, official food control, and research facilities. Mean recoveries reported ranged from 89% (at 120 microg/kg) to 85% (at 240 microg/kg) for baby food and from 100% (at 200 microg/kg) to 93% (at 400 microg/kg) for animal feed. On the basis of the results for spiked samples (blind duplicates at 2 levels), as well as those for naturally contaminated samples (blind duplicates at 3 levels), the relative standard deviation for repeatability (RSDr) in analyses of baby food ranged from 6.4 to 14.0% and in analyses of animal feed, from 6.1 to 16.5%. The relative standard deviation for reproducibility (RSDR) in analyses of baby food ranged from 9.4 to 19.5% and in analyses of animal feed, from 10.5 to 25.2%. The HorRat values ranged from 0.4 to 1.0 and from 0.7 to 1.3, for baby food and animal feed, respectively. The method showed acceptable performance for within-laboratory and between-laboratory precision for each matrix, as required by European legislation.
Stroka
Joerg
J
European Commission-Joint Research Centre, Institute for Reference Materials and Measurements, Food Safety and Quality Unit, Retieseweg 111, B-2440 Geel, Belgium. Joerg.Stroka@cec.eu.int
Derbyshire
Michelle
M
Mischke
Carsten
C
Ambrosio
Massimo
M
Kroeger
Katy
K
Arranz
Isabel
I
Sizoo
Eric
E
van Egmond
Hans
H
D000821:Animal Feed / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D007225:Infant Food / Q000032:analysis*; D015233:Models, Statistical; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014255:Trichothecenes / Q000032:analysis*
D014255:Trichothecenes; C007262:deoxynivalenol