The IE2 promoter/enhancer region from mouse CMV provides high levels of therapeutic protein expression in mammalian cells.
Protein expression in mammalian cells is key for the production and manufacturing of bio-therapeutics with human-like properties and activities. As a molecular basis for reaching high protein expression levels, efficient promoter/enhancer systems are a prerequisite. Here we identify a novel enhancer from the mouse cytomegalovirus (CMV) immediate early 2 (IE2) region as a strong expression-promoting element. We further demonstrate its activity in bi-directional promoter architecture and apply it to generate production clones for IL-18BP, a protein with therapeutic indications in autoimmune diseases. These data show that the IE region from mouse CMV, and the IE2 enhancer/promoter in particular, have a broad potential for application in novel gene expression systems for research, development, and manufacturing of protein drugs.
Chatellard
Philippe
P
Cell Sciences, Laboratoires Serono SA, CH-1809 Fenil-sur-Corsier, Switzerland.
Pankiewicz
Renata
R
Meier
Evelyne
E
Durrer
Laurence
L
Sauvage
Christophe
C
Imhof
Markus O
MO
D000818:Animals; D016466:CHO Cells; D003001:Cloning, Molecular / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000627:therapeutic use; D051379:Mice; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*
C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; D015534:Trans-Activators; C118370:interleukin-18 binding protein