title: Intracellular penetration and activity of DX-619 in human polymorphonuclear leukocytes.
abstract: The intracellular penetration and activity of DX-619 in human polymorphonuclear leukocytes have been evaluated. DX-619 reached intracellular concentrations 10 times higher than the extracellular concentrations reached. Uptake was rapid, reversible, nonsaturable, and affected by environmental temperature, some metabolic inhibitors, and a soluble membrane activator. DX-619 showed intracellular activity against Staphylococcus aureus.
authors.affiliation: Department of Microbiology, School of Medicine, University of Seville, Seville-41009, Spain. igarcial@us.es
authors.forename: Isabel
authors.initials: I
authors.lastname: García
authors.forename: Sofía
authors.initials: S
authors.lastname: Ballesta
authors.forename: Concepción
authors.initials: C
authors.lastname: Murillo
authors.forename: Evelio J
authors.initials: EJ
authors.lastname: Perea
authors.forename: Alvaro
authors.initials: A
authors.lastname: Pascual
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D011759:Pyrrolidines / Q000097:blood* / Q000493:pharmacokinetics; D015363:Quinolones / Q000097:blood* / Q000493:pharmacokinetics; D013203:Staphylococcal Infections / Q000097:blood / Q000188:drug therapy / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: C505440:DX 619; D011759:Pyrrolidines; D015363:Quinolones