title: High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell culture.
abstract: Developing an effective vaccine against enterovirus 71 (EV71) infection provides the best means to control the disease. We have previously reported that large-scale preparation of a low immunogenic EV71 strain can be achieved using serum free microcarrier Vero cell culture in a 2-l bioreactor [Wu SC, Liu CC, Lian WC. Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine development. Vaccine 2004;22:3858-64]. This present work further investigated the virus growth and the immunogenicity of two high immunogenic strains (EV71-075 and EV71-117) prepared in serum-free microcarrier cell cultures. Our results showed that serum free culture increased cell death rate after infection, reduced the virus specific productivity, but resulted in elicitation of higher neutralizing titers in immunized mice as compared to that parallel obtained in serum-containing cultures. Therefore, serum-free microcarrier culture is a valuable process for developing inactivated EV71 vaccines.
authors.affiliation: Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan.
authors.forename: Chia-Chyi
authors.initials: CC
authors.lastname: Liu
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Lian
authors.forename: Michael
authors.initials: M
authors.lastname: Butler
authors.forename: Suh-Chin
authors.initials: SC
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019149:Bioreactors; D002522:Chlorocebus aethiops; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004770:Enterovirus / Q000145:classification / Q000254:growth & development* / Q000276:immunology* / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000517:prevention & control; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D008863:Microspheres; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D044967:Serum; D015164:Vaccines, Inactivated; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014765:Viral Vaccines; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D003470:Culture Media; D016895:Culture Media, Serum-Free; D015164:Vaccines, Inactivated; D014764:Viral Proteins; D014765:Viral Vaccines