[Effect of p, p'-DDE on DNA damage and expression of FasL gene of rat Sertoli cell in vitro].
OBJECTIVE
To study the effects of p, p'-DDE on DNA damage and expression of FasL gene of rat Sertoli cell in vitro.


METHODS
After separating Sertoli cells from testicular tissue of rats, we cultured these cells for 3 days and then added different doses of p, p'-DDE into the culture medium to study its effect on DNA damage and FasL gene expression with the method of SCGE and RT-PCR.


RESULTS
DNA migration and FasL gene expression of Sertoli cell significantly increased with the increase of the dose of p, p'-DDE.


CONCLUSIONS
p, p'-DDE could induce DNA damage and FasL gene expression of Sertoli cells of rat testis. p, p'-DDE might induce the Sertoli cell damage and destroy the dynamic process of spermatogenesis through the Fas/FasL pathway, which might lead to oligozoospermatism.
Song
Yang
Y
Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Yang
Ke-Di
KD
D000818:Animals; D002478:Cells, Cultured; D004249:DNA Damage; D003633:Dichlorodiphenyl Dichloroethylene / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity*; D053222:Fas Ligand Protein / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects*
D052244:Endocrine Disruptors; D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D007306:Insecticides; D003633:Dichlorodiphenyl Dichloroethylene