Determination of the biliary excretion of piperacillin in humans using a novel method.
OBJECTIVE
To evaluate the applicability of a novel method to determine the biliary excretion of piperacillin.


METHODS
Healthy volunteers were administered piperacillin i.v. Duodenal aspirates were collected via a custom-made oroenteric catheter; blood and urine also were collected. Gallbladder ejection fraction (EF) was determined by gamma scintigraphy and pharmacokinetic parameters were calculated using noncompartmental analysis.


RESULTS
The fraction of the piperacillin dose excreted unchanged into bile was 1.1 +/- 0.3% (biliary clearance corrected for EF was 0.032 +/- 0.008 ml min(-1) kg(-1)).


CONCLUSIONS
This methodology can be used to determine reliably the biliary clearance of drugs that are excreted only marginally into bile. Normalization of biliary clearance for EF significantly reduces intersubject variability of this parameter.
Ghibellini
Giulia
G
School of Pharmacy, University of North Carolina at Chapel hill, Chapel Hill, NC, USA.
Vasist
Lakshmi S
LS
Hill
Terence E
TE
Heizer
William D
WD
Kowalsky
Richard J
RJ
Brouwer
Kim L R
KL
D000328:Adult; D000814:Aniline Compounds; D001646:Bile / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005765:Gastrointestinal Agents / Q000494:pharmacology; D005998:Glycine; D006801:Humans; D007098:Imino Acids; D008297:Male; D015609:Organotechnetium Compounds; D010878:Piperacillin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D019275:Radiopharmaceuticals; D012844:Sincalide / Q000494:pharmacology
D000814:Aniline Compounds; D005765:Gastrointestinal Agents; D007098:Imino Acids; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C034837:technetium Tc 99m mebrofenin; D012844:Sincalide; D005998:Glycine; D010878:Piperacillin