[Effect of Sudan I, III and IV on proliferation of HepG-2 and SGC-7901].
To study the effect of sudan I, III and IV on the growth of HepG-2 and SGC-7901, the ratio of DNA/RNA and 3D image in HepG-2 treated by different dose of sudan I, III and IV was detected by LCM, the content change of DNA and the cell cycle in SGC-7901 treated by different dose of sudan I, III and IV was detected by flow cytometry. Results showed the ratio of DNA/RNA in control group was 1.2232 +/- 0.0844, after treated by sudan I , III and IV the fluorescence intensity of DNA was stronger than RNA, and the low dose group was very marked compared with control group (p < 0.01), the ratio was 1.609 6 +/- 0.1990, 1.4455 +/- 0.1633,and 1.7081 +/- 0.1090. 3D image showed that the DNA fluorescence mostly assembles on nucleolus, which was denser and stronger than RNA. Sudan I, III and IV accelerated the cell cycle from G1 phase to S and G2 phase, the average content of DNA in G1 phase decreased and in S and G2 phase increased, the index of PI and SPF increased. Sudan I, III and IV could affect cell cycle and advanced its differentiation and proliferation.
Ji
Yu-bin
YB
Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, China. jyb@hrbcu.edu.cn
Ji
Chen-feng
CF
Gao
Shi-yong
SY
Lang
Lang
L
Yu
Lei
L
D017209:Apoptosis / Q000187:drug effects; D001391:Azo Compounds / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004396:Coloring Agents / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009284:Naphthols / Q000494:pharmacology*; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology
D001391:Azo Compounds; D004396:Coloring Agents; D004273:DNA, Neoplasm; D009284:Naphthols; D012334:RNA, Neoplasm; C024336:1-phenylazo-2-naphthol; C009213:Scarlet Red; C033006:sudan III