title: Sequence analysis and transcriptional profiling of two vacuolar H+ -pyrophosphatase isoforms in Vitis vinifera.
abstract: Gene expression of grapevine vacuolar H(+)-pyrophosphatase (V-PPase EC 3.6.1.1.) during fruit ripening has previously been reported. Here we report on putative multiple V-PPase isoforms in grapevine. In this study a full-length cDNA sequence with an open reading frame of 2,295 nucleotides encoding a V-PPase gene (vpp2: acc. nr. AJ557256) was cloned. Sequence analyses of the deduced amino acid residues and RT-PCR experiments indicated that Vitis vinifera L. has at least two distinct isoforms of the V-PPase gene. Bioinformatic analyses of 13 V-PPase protein sequences revealed two highly conserved motifs associated with pyrophosphate (PPi) binding and response to stress, respectively. Both V-PPase isoforms were expressed at higher levels in the late post-véraison stage of grape berry ripening. Results also showed that the expression of grapevine V-PPase was induced by cold stress.
authors.affiliation: Institute for Plant Biotechnology, Stellenbosch University, Private Bag X1, Matieland, South Africa. plantb7@sun.ac.za
authors.forename: Mauritz
authors.initials: M
authors.lastname: Venter
authors.forename: Jan-Hendrik
authors.initials: JH
authors.lastname: Groenewald
authors.forename: Frederik C
authors.initials: FC
authors.lastname: Botha
mesh_terms: D000595:Amino Acid Sequence; D018744:DNA, Plant / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D043564:Inorganic Pyrophosphatase / Q000737:chemistry / Q000235:genetics*; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D008293:Malates / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013395:Sucrose / Q000378:metabolism; D014158:Transcription, Genetic; D014617:Vacuoles / Q000201:enzymology*; D027843:Vitis / Q000201:enzymology* / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D007527:Isoenzymes; D008293:Malates; D012333:RNA, Messenger; D005632:Fructose; D013395:Sucrose; C030298:malic acid; D043564:Inorganic Pyrophosphatase; D005947:Glucose