Prostaglandin D2 and J2-series (PGJ2, Delta12-PGJ2) prostaglandins stimulate IL-6 and MCP-1, but inhibit leptin, expression and secretion by 3T3-L1 adipocytes.
Prostaglandin D(2) and its derivatives PGJ(2) and Delta(12)-PGJ(2) strongly stimulate the synthesis and secretion by white adipocytes of the neurotrophin NGF. Here we have explored whether PGD(2) and the J(2)-series prostaglandins have pervasive effects on adipokine production. The influence of these prostaglandins on the production of the adipocyte hormones leptin and adiponectin, and the inflammatory factors IL-6 and monocyte chemoattractant protein 1 (MCP-1), were examined in 3T3-L1 adipocytes. PGD(2) induced a reduction in adiponectin and leptin mRNA, and the secretion of these adipokines was also inhibited, the effect being greater with leptin (up to 10-fold) than with adiponectin (twofold). In contrast, PGD(2) induced a marked stimulation of IL-6 and MCP-1 expression; with IL-6, this was rapid, the mRNA level increasing by >50-fold by 1 h. The rise in mRNA was accompanied by an increase in IL-6 and MCP-1 release (up to 100- and 6.5-fold, respectively). The effects of PGD(2) were generally mirrored by PGJ(2) and Delta(12)-PGJ(2); Delta(12)-PGJ(2) was a particularly strong stimulator of IL-6 production. These results indicate that PGD(2) and the J(2)-series prostaglandins PGJ(2) and Delta(12)-PGJ(2) can have major effects on the synthesis and release of key adipokines. Such effects could be important in the inflammatory response in adipose tissue.
Peeraully
Muhammad R
MR
Obesity Biology Unit, Liverpool Centre for Nutritional Genomics and Liverpool Obesity Research Network, Division of Metabolic and Cellular Medicine, University of Liverpool, Duncan Building, Liverpool, UK.
Sievert
Henning
H
Bulló
Mònica
M
Wang
Bohan
B
Trayhurn
Paul
P
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism / Q000502:physiology*; D052242:Adiponectin / Q000378:metabolism; D000818:Animals; D018932:Chemokine CCL2 / Q000378:metabolism*; D015870:Gene Expression; D015850:Interleukin-6 / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism*; D051379:Mice; D015230:Prostaglandin D2 / Q000031:analogs & derivatives / Q000502:physiology*; D013997:Time Factors
D052242:Adiponectin; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D015850:Interleukin-6; D020738:Leptin; C499810:delta(12)-prostaglandin J(2); C037112:9-deoxy-delta-9-prostaglandin D2; D015230:Prostaglandin D2