Identification of a novel glycosyltransferase involved in LOS biosynthesis of Moraxella catarrhalis.
Moraxella catarrhalis is an important human mucosal pathogen that contributes to otitis media in infants and exacerbates conditions such as chronic obstructive pulmonary disease in the elderly. This study describes the identification of a novel gene, lgt5 that encodes a glycosyltransferase involved in the LOS biosynthesis of M. catarrhalis. Analysis of NMR data of LOS-derived oligosaccharide from a Serotype A lgt5 mutant strain of M. catarrhalis indicate that lgt5 encodes an alpha-(1-->4)-galactosyltransferase.
Wilson
Jennifer C
JC
Institute for Glycomics, Griffith University, Gold Coast, 4215 QLD, Australia.
Collins
Patrick M
PM
Klipic
Zoran
Z
Grice
I Darren
ID
Peak
Ian R
IR
D000942:Antigens, Bacterial / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D017931:DNA Primers; D005838:Genotype; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D001936:Moraxella catarrhalis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D011994:Recombinant Proteins / Q000378:metabolism
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; C023023:lipid-linked oligosaccharides; D005964:Glucosyltransferases; C114421:lipooligosaccharide beta chain synthesizing glucosyltransferase