title: Colorectal mucosal histamine release by mucosa oxygenation in comparison with other established clinical tests in patients with gastrointestinally mediated allergy.
abstract: OBJECTIVE
  This study evaluated colorectal mucosal histamine release in response to blinded food challenge-positive and -negative food antigens as a new diagnostic procedure.
  
  
  METHODS
  19 patients suffering from gastrointestinally mediated allergy confirmed by blinded oral provocation were investigated on grounds of their case history, skin prick tests, serum IgE detection and colorectal mucosal histamine release by ex vivo mucosa oxygenation. Intact tissue particles were incubated/stimulated in an oxygenated culture with different food antigens for 30 min. Specimens challenged with anti-human immunoglobulin E and without any stimulus served as positive and negative controls, respectively. Mucosal histamine release (% of total biopsy histamine content) was considered successful (positive), when the rate of histamine release from biopsies in response to antigens reached more than twice that of the spontaneous release. Histamine measurement was performed by radioimmunoassay.
  
  
  RESULTS
  The median (range) of spontaneous histamine release from colorectal mucosa was found to be 3.2 (0.1%-25.8%) of the total biopsy histamine content. Food antigens tolerated by oral provocation did not elicit mast cell degranulation 3.4 (0.4%-20.7%, P = 0.4), while anti-IgE and causative food allergens induced a significant histamine release of 5.4 (1.1%-25.6%, P = 0.04) and 8.1 (1.5%-57.9%, P = 0.008), respectively. 12 of 19 patients (63.1%) showed positive colorectal mucosal histamine release in accordance with the blinded oral challenge responding to the same antigen (s), while the specificity of the functional histamine release to accurately recognise tolerated foodstuffs was found to be 78.6%. In comparison with the outcome of blinded food challenge tests, sensitivity and specificity of history (30.8% and 57.1%), skin tests (47.4% and 78.6%) or antigen-specific serum IgE determinations (57.9% and 50%) were found to be of lower diagnostic accuracy in gastrointestinally mediated allergy.
  
  
  CONCLUSIONS
  Functional testing of the reactivity of colorectal mucosa upon antigenic stimulation in patients with gastrointestinally mediated allergy is of higher diagnostic efficacy.
authors.affiliation: Functional Tissue Diagnostics, Gastroenterology, Department of Medicine I, University Erlangen-Nuremberg, Ulmenweg 18, Erlangen 91054, Germany. martin.raithel@med1.imed.uni-erlangen.de
authors.forename: M
authors.initials: M
authors.lastname: Raithel
authors.forename: M
authors.initials: M
authors.lastname: Weidenhiller
authors.forename: R
authors.initials: R
authors.lastname: Abel
authors.forename: H W
authors.initials: HW
authors.lastname: Baenkler
authors.forename: E G
authors.initials: EG
authors.lastname: Hahn
mesh_terms: D000328:Adult; D001706:Biopsy; D005260:Female; D005512:Food Hypersensitivity / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000276:immunology*; D006632:Histamine / Q000378:metabolism*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D007159:Immunologic Tests / Q000379:methods; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012882:Skin Tests / Q000379:methods*
chemical_list: D007073:Immunoglobulin E; D006632:Histamine