[The concentration and expression of IL-4, IL-5, IL-6, IL-8 in human nasal polyps tissues].
OBJECTIVE
To study the concentration and expression of IL-4, IL-5, IL-6, IL-8 in human nasal polyps tissues, and to explore the relationship between cytokines and the formative mechanism of nasal polyps.


METHODS
The concentration and expression of IL-4, IL-5, IL-6, IL-8 in 54 cases with nasal polyps were determined by enzyme linked immunosorbent assay (ELISA) and immunohistochemistry, and the middle turbinate mucosa from 22 patients with the deviation of nasal septum were used as the control.


RESULTS
The concentration of IL-5, IL-8 in nasal polyps tissues were significantly higher than that in normal middle turbinate mucosa (P < 0.01). There was no significant difference between the concentration of IL-6 in nasal polyps and that in normal control group (P > 0.05). No significant difference was found between the concentration of IL-4 in nasal polyps tissues with negative allergic skin test and that in control group, but the concentration of IL-4 was significantly higher in nasal polyps tissues with positive allergic skin test than in control group (P < 0.05). IL-4 was mostly found in lymphocytes and plasma cells in nasal polyps and IL-5 in eosinophils and lymphocytes. IL-6 and IL-8 were mostly found in nasal polyps epithelium and inflammatory cells, as was no difference between positive allergic skin test and negative one.


CONCLUSIONS
IL-5 and IL-8 are the key cytokines in the formation of nasal polyps, and IL-4 is a key cytokine in nasal polyps with positive allergic skin test. IL-6 isn't an important cytokine in the formative mechanism of nasal polyps.
Yue
Jianxin
J
Department of Otolaryngology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China. yjxthfll@hotmail.com
Chen
Jianjun
J
Kong
Weijia
W
Tan
Hui
H
Shu
Hong
H
Shi
Qiumei
Q
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism*; D015848:Interleukin-5 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism*; D055815:Young Adult
C508606:CXCL8 protein, human; C508598:IL4 protein, human; C508599:IL5 protein, human; C508600:IL6 protein, human; D015848:Interleukin-5; D015850:Interleukin-6; D016209:Interleukin-8; D015847:Interleukin-4