title: MarA-like regulator of multidrug resistance in Yersinia pestis.
abstract: MarA47(Yp) from Yersinia pestis, showing 47% identity to Escherichia coli MarA in its N terminus, caused resistance to antibiotics and to organic solvents when expressed in both E. coli and Y. pestis. Resistance was linked to increased expression of the AcrAB multidrug efflux pump. In four of five spontaneous multidrug-resistant mutants of Y. pestis independently selected by growth on tetracycline, the marA47(Yp) gene was overexpressed. The findings suggest that marA47(Yp) is a marA ortholog in Y. pestis.
authors.affiliation: Center for Adaptation Genetics and Drug Resistance, Department of Molecular Biology, Tufts University School of Medicine, Boston, MA 02111, USA.
authors.forename: Rupa A
authors.initials: RA
authors.lastname: Udani
authors.forename: Stuart B
authors.initials: SB
authors.lastname: Levy
mesh_terms: D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D008074:Lipoproteins / Q000096:biosynthesis*; D026901:Membrane Transport Proteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D015010:Yersinia pestis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: C083497:AcrA protein, E coli; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; C080002:MarA protein, E coli; D026901:Membrane Transport Proteins