title: Absolute configuration of hydroxycitric acid produced by microorganisms.
abstract: Optical resolution for (2S,3R) and (2R,3S)-hydroxycitric acid (HCA) enantiomers was developed using chiral column chromatography. HCA from Bacillus megaterium G45C and Streptomyces sp. U121, newly isolated in our previous study, was analyzed to determine the absolute configuration. These results indicate that both strains generate optically pure (2S,3R)-hibiscus type HCA enantiomer.
authors.affiliation: Department of Applied Biological Science, Faculty of Life Science and Biotechnology, Fukuyama University, Hiroshima, Japan. yamada@fubac.fukuyama-u.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hida
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamada
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yamada
mesh_terms: D001410:Bacillus megaterium / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002951:Citrates / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008968:Molecular Conformation; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013302:Streptomyces / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002951:Citrates; C007999:hydroxycitric acid