Antibiotic resistance and genetic diversity of Shigella sonnei isolated from patients with diarrhoea between 1999 and 2003 in Bangladesh.
Shigella sonnei is a significant cause of diarrhoeal infection in both developing and industrialized countries. From 1999 to 2003, 445 strains of Shigella sonnei were isolated from patients admitted to the diarrhoea treatment centre of the International Center for Diarrhoeal Disease Research, Bangladesh. More than 60% of the isolates were resistant to nalidixic acid, 89% to sulfamethoxazole-trimethoprim and 9.5% to ampicillin. In addition, 4% of strains were resistant to multiple antibiotics (AmpR TetR SxtR StrR) and 4.2% of strains were sensitive to all antibiotics tested. None of the strains were positive for the set1 gene, whereas 46% were positive for the sen gene. Forty-six per cent of the strains (stored at -70 degrees C) harboured the 120 MDa invasive plasmid and representative strains produced keratoconjunctivitis in the guinea pig eye. In addition, three plasmids of approximately 5, 1.8 and 1.4 MDa were found to be present in more than 90% of the strains. A self-transmissible, middle-ranged plasmid (35-80 MDa) carrying the multiple antibiotic resistance gene was found in some strains. PFGE analysis of the strains identified five unique types with many subtypes, which were characterized into four unique types by ribotyping analysis. It can be concluded that endemic strains of Shigella sonnei isolated from patients in Bangladesh are diverse in their genetic pattern.
Talukder
Kaisar A
KA
ICDDR, B, Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
Islam
Zhahirul
Z
ICDDR, B, Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
Dutta
Dilip K
DK
ICDDR, B, Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
Islam
M Aminul
MA
ICDDR, B, Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
Khajanchi
Bijay K
BK
ICDDR, B, Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
Azmi
Ishrat J
IJ
ICDDR, B, Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
Iqbal
Mohd S
MS
ICDDR, B, Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
Hossain
M A
MA
ICDDR, B, Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
Faruque
A S G
ASG
ICDDR, B, Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
Nair
G Balakrish
GB
ICDDR, B, Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
Sack
David A
DA
ICDDR, B, Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
D000818:Animals; D001459:Bangladesh; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology*; D004195:Disease Models, Animal; D024881:Drug Resistance, Bacterial; D004405:Dysentery, Bacillary / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D004768:Enterotoxins / Q000235:genetics; D014644:Genetic Variation; D006168:Guinea Pigs; D006801:Humans; D007637:Keratoconjunctivitis / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D010957:Plasmids; D021521:Ribotyping; D012764:Shigella sonnei / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence
D004269:DNA, Bacterial; D004768:Enterotoxins