title: Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas aeruginosa with fatty acids and alternative carbon sources.
abstract: In this study, medium-chain-length polyhydroxyalkanoates (mcl-PHAs) were produced by Pseudomonas aeruginosa using different carbon sources. Decanoic acid induced the highest (9.71% [+/- 0.7]) mcl-PHAs accumulation in bacterial cells at 47 h. The cells preferred to accumulate and degrade the polyhydroxyoctanoate than polyhydroxydecanoate (PHD) during early stage and final stage of the growth, respectively. The production cost of mcl-PHAs can be reduced by using edible oils as the carbon source. The bacteria accumulated 6% (+/- 0.7) of mcl-PHAs in the presence of olive oil. Besides, reused oil was another potential carbon source for the reduction of the production cost of mcl-PHAs. Overall, PHD was the major constituent in the accumulated mcl-PHAs.
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China.
authors.forename: Pui-Ling
authors.initials: PL
authors.lastname: Chan
authors.forename: Vincent
authors.initials: V
authors.lastname: Yu
authors.forename: Lam
authors.initials: L
authors.lastname: Wai
authors.forename: Hoi-Fu
authors.initials: HF
authors.lastname: Yu
mesh_terms: D019149:Bioreactors / Q000191:economics / Q000382:microbiology; D002244:Carbon / Q000191:economics / Q000378:metabolism; D049109:Cell Proliferation; D002681:China; D005227:Fatty Acids / Q000191:economics* / Q000378:metabolism*; D008970:Molecular Weight; D010938:Plant Oils / Q000191:economics* / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000191:economics* / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D010938:Plant Oils; D011108:Polymers; C111948:poly(hydroxyethanoate); D002244:Carbon