title: Effects of gradual coronary artery occlusion and exercise training on gene expression in swine heart.
abstract: Gradual occlusion (O) of the swine left circumflex coronary artery (LCX) with an ameroid occluder results in complete O within 3 weeks, collateral vessel development, and compensatory hypertrophy. The purpose of this investigation was to determine the independent and combined effects of O and exercise training (E) on gene expression in the swine heart. Adult Yucatan miniature swine were assigned to one of the following groups (n=6-9/group): sedentary control (S), exercise-trained (E), sedentary swine subjected to LCX occlusion (SO), and exercise-trained swine with LCX occlusion (EO). Exercise consisted of progressive treadmill running conducted 5 d/wk for 16 weeks. Gene expression was studied in myocardium isolated from the collateral-dependent left ventricle free wall (LV) and the collateral-independent septum (SEP) by RNA blotting. E and O each stimulated cardiac hypertrophy independently (p<0.001) with no interaction. O but not E increased atrial natriuretic factor expression in the LV, but not in the SEP. E decreased the expression of beta-myosin heavy chain in the LV, but not in the SEP. E retarded the expression of collagen III mRNA in SEP; but not in the LV. Exercise training and coronary artery occlusion each stimulate cardiac hypertrophy independently and induce different patterns of gene expression.
authors.affiliation: University of Michigan, Ann Arbor, MI, USA. boluytm@umich.edu
authors.forename: Marvin O
authors.initials: MO
authors.lastname: Boluyt
authors.forename: Georgina M
authors.initials: GM
authors.lastname: Cirrincione
authors.forename: Amy M
authors.initials: AM
authors.lastname: Loyd
authors.forename: Donna H
authors.initials: DH
authors.lastname: Korzick
authors.forename: Janet L
authors.initials: JL
authors.lastname: Parker
authors.forename: M Harold
authors.initials: MH
authors.lastname: Laughlin
mesh_terms: D000818:Animals; D024061:Collagen Type III / Q000378:metabolism; D003326:Coronary Circulation / Q000502:physiology*; D003327:Coronary Disease / Q000503:physiopathology*; D003331:Coronary Vessels / Q000503:physiopathology; D005080:Exercise Test; D005260:Female; D015870:Gene Expression; D008297:Male; D010805:Physical Conditioning, Animal; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D034421:Sus scrofa; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors
chemical_list: D024061:Collagen Type III; D012333:RNA, Messenger