title: Comparison between the Gen-Probe transcription-mediated amplification Trichomonas vaginalis research assay and real-time PCR for Trichomonas vaginalis detection using a Roche LightCycler instrument with female self-obtained vaginal swab samples and male urine samples.
abstract: This study compared two assays for Trichomonas vaginalis detection, Gen-Probe's transcription-mediated amplification (TMA) assay for Trichomonas vaginalis and BTUB FRET PCR, using self-obtained clinical samples from 611 patients. Infection status was defined as two positive results by two different tests. The initial TMA assay sensitivity was 96.7%; specificity was 97.5%. The TMA assay was comparable to BTUB FRET PCR.
authors.affiliation: Division of Infectious Diseases, School of Medicine, Johns Hopkins University, 1159 Ross Building, 720 Rutland Ave., Baltimore, MD 21015, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Hardick
authors.forename: Justin
authors.initials: J
authors.lastname: Hardick
authors.forename: Billie Jo
authors.initials: BJ
authors.lastname: Wood
authors.forename: Charlotte
authors.initials: C
authors.lastname: Gaydos
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D012680:Sensitivity and Specificity; D014246:Trichomonas vaginalis / Q000302:isolation & purification*; D014621:Vagina / Q000469:parasitology*