A novel copper-hydrogen peroxide formulation for prion decontamination.
With the appearance of variant Creutzfeldt-Jakob disease (CJD) and the detection of infectious prions in the peripheral organs of persons with sporadic CJD, the development of decontamination methods that are compatible with medical equipment has become a major issue. Here, we show that a formulation of copper metal ions in combination with hydrogen peroxide dramatically reduces the level of prion protein (PrP)(Sc) (the scrapie isoform of PrP) present in homogenates of samples from prion-infected brains, including brain samples from humans with CJD. An animal bioassay confirmed the reduction in prion infectivity, indicating that this novel Cu(2+)-H(2)O(2) formulation has great potential for prion decontamination.
Solassol
Jerome
J
Institut de Genetique Humaine du Centre National de la Recherche Scientifique, UPR1142, 34396 Montpellier, France. j-solassol@chu-montpellier.fr
Pastore
Manuela
M
Crozet
Carole
C
Perrier
Veronique
V
Lehmann
Sylvain
S
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D001681:Biological Assay; D001921:Brain / Q000382:microbiology; D003300:Copper / Q000494:pharmacology*; D007562:Creutzfeldt-Jakob Syndrome / Q000517:prevention & control; D003666:Decontamination / Q000379:methods*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D017096:Prion Diseases / Q000382:microbiology / Q000517:prevention & control*; D011328:Prions / Q000187:drug effects*; D012608:Scrapie / Q000517:prevention & control; D014131:Trace Elements / Q000494:pharmacology
D000891:Anti-Infective Agents, Local; D011328:Prions; D014131:Trace Elements; D003300:Copper; D006861:Hydrogen Peroxide