Effects of hyperthermia on heat shock protein expression, alkaline phosphatase activity and proliferation in human osteosarcoma cells.
Hyperthermia can be used as a possible adjuvant therapy in treatment of cancer patients. In this study, the direct effect of hyperthermia on osteosarcoma derived cell lines HOS85, MG-63 and SaOS-2 was investigated. Heat shock at 42 degrees C inhibited proliferation significantly in all three cell lines tested. Furthermore a sub-lethal heat shock (42 degrees C, 1 h) decreases alkaline phosphatase activity, the absolute marker for osteoblast-like cells, in all of the three cell lines. Hsp70 was expressed constitutively and was found to be upregulated in a time-dependent manner; by up to 150% in Western blot analysis. The results of this study indicate that heat shock has an inhibitory effect on human osteosarcoma cells. These data suggest that hyperthermia has an anti-tumour effect on cancers of the bone and might, therefore, become an adjuvant treatment option.
Trieb
K
K
Department of Orthopedics, University of Vienna, Waehringerguertel 18-20, Vienna, Austria. k.trieb.co@klinikumffo.de
Blahovec
H
H
Kubista
B
B
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology
D006360:Heat-Shock Proteins; D000469:Alkaline Phosphatase