Expression and functional roles of the pepper pathogen-induced transcription factor RAV1 in bacterial disease resistance, and drought and salt stress tolerance.
A novel pathogen-induced gene encoding the RAV (Related to ABI3/VP1) transcription factor, CARAV1, was isolated from pepper leaves infected with Xanthomonas campestris pv. vesicatoria. CARAV1 contains two distinct DNA-binding domains AP2 and B3 uniquely found in higher plants. Transient expression analysis of the smGFP:CARAV1 fusion construct in Arabidopsis protoplasts and pepper epidermal cells revealed the CARAV1 protein to be localized in the nucleus. The N-terminal region of CARAV1 fused to the GAL4 DNA-binding domain was required to activate transcription of reporter genes in yeast. In yeast one-hybrid, the recognition of CAACA and CACCTG motifs also were essential for the CARAV1 protein to bind to a specific target gene and activate the reporter gene. The expression of the CARAV1 gene was strongly induced early in pepper leaves during the pathogen infection, abiotic elicitors and environmental stresses. CARAV1 transcripts were localized in the phloem cells of leaf tissues during pathogen infection and ethylene treatment. Ectopic expression of the CARAV1 gene in transgenic Arabidopsis plants induced some PR genes and enhanced resistance against infection by Pseudomonas syringae pv. tomato DC3000 and osmotic stresses by high salinity and dehydration. The CARAV1 promoter activation was induced by P. syringae pv. tabaci, salicylic acid and abscisic acid. These data suggest that pathogen- and abiotic stress-inducible CARAV1 functions as a transcriptional activator triggering resistance to bacterial infection and tolerance to osmotic stresses.
Sohn
Kee Hoon
KH
Laboratory of Molecular Plant Pathology, College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, Republic of Korea.
Lee
Sung Chul
SC
Jung
Ho Won
HW
Hong
Jeum Kyu
JK
Hwang
Byung Kook
BK
D000040:Abscisic Acid / Q000494:pharmacology; D000085:Acetates / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000235:genetics; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000382:microbiology; D002212:Capsicum / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology; D003517:Cyclopentanes / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics*; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007113:Immunity, Innate / Q000235:genetics; D017403:In Situ Hybridization; D008353:Mannitol / Q000494:pharmacology; D008969:Molecular Sequence Data; D054883:Oxylipins; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics*; D014867:Water / Q000378:metabolism / Q000494:pharmacology; D016959:Xanthomonas campestris / Q000254:growth & development
D000085:Acetates; D003517:Cyclopentanes; D004268:DNA-Binding Proteins; D005030:Ethylenes; D054883:Oxylipins; D010940:Plant Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D014867:Water; D008353:Mannitol; D012965:Sodium Chloride; D000040:Abscisic Acid; C072239:methyl jasmonate; C036216:ethylene; D006861:Hydrogen Peroxide