title: Quantification and stability studies on the flavonoids of Radix hedysari.
abstract: Radix hedysari is not only a restorative food but also a famous Traditional Chinese Medicine. In this study, a simple, reliable, and reproducible high-performance liquid chromatography-ultraviolet method was developed for the first time to determine the true contents of five major flavoniods, naringenin-7-O-alpha-L-rhamanopyranosyl-(1-->2)-beta-d-glucopyranoside, ononin, formononetin, medicarpin, and an unstable flavonoid malonate (formononetin-7-O-beta-D-glucoside-6' '-O-malonate, FGM), and the stability of FGM was also investigated. Flavoniods were selected as chemical markers because they have appeared as one of the major bioactive compound groups in Radix hedysari. The stability results of FGM at different pH showed that it remained relatively stable in acidic aqueous methanol of pH 3. The HPLC assay with an improved sample preparation scheme can be readily utilized as a suitable "chemical" quality control method for Radix hedysari.
authors.affiliation: Department of Natural Medicines and State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100083, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Hu-Biao
authors.initials: HB
authors.lastname: Chen
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Zhao
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Qing-Ying
authors.initials: QY
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Pengfei
authors.initials: P
authors.lastname: Tu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D004365:Drugs, Chinese Herbal; D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids