[Characterization of enzymatic degradation of microcystins by a new isolated bacterium].
A strain of bacterium capable of biodegrading microcystin (MC) RR and MC-LR was isolated from the sediments of Dianchi Lake. It was demonstrated that the enzymes in cell-free extract of this bacterium were responsible for the degradation of MC-RR and MC-LR, and the dead-end products of MC-RR and MC-LR catalyzed by these enzymes were observed on HPLC chromatograms. Results show that the optimum pH for the activities of these enzymes was in the range from 6.0 to 8.0, however the metal ions of Cu2+, Mn2+ and Zn2+ had no apparent effects on the enzymatic degradation of MC-RR and MC-LR.
He
Hong-sheng
HS
Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, China. hhsheng00@163.com
Yan
Hai
H
Zhou
Jie
J
Ge
Shi-you
SY
Xiao
Bao-qing
BQ
Lü
Le
L
D001419:Bacteria / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D019015:Geologic Sediments / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D008387:Marine Toxins; D052998:Microcystins / Q000378:metabolism*; D014871:Water Microbiology
D001426:Bacterial Proteins; D008387:Marine Toxins; D052998:Microcystins; C063855:microcystin RR; C057862:cyanoginosin LR