[The influence of soybean isoflavone on expression of low density lipoprotein receptor (LDLR) mRNA in ovariectomied rats].
OBJECTIVE
To investigate the effect of soybean isoflavone on blood lipid and the expression of LDLR mRNA in ovariectomied rats.


METHODS
Forty eight Sprague-Dawley rats were divided randomly into four groups: Sham group (sham), OVX group (OVX), OVX + gemifibrozil (G) group (OVX + G) and OVX + isoflavone (ISO) group (OVX_ISO). in which the rats were treated with G or ISO for three months starting from two weeks after both sides of rat' s ovarietomy. Blood sample were taken out for determination of blood lipid. The liver tissue were taken out quickly, Isothiocyanate guanidine-phenol-chloroform was used to extract the total RNA from the liver and RT-PCR was used to detect the expression of LDLR mRNA.


RESULTS
Compared with the OVX group, contents of TG, LDL-C and OX-LDL in serum in OVX + ISO group decreased remarkably, and the contents of HDL-C increased. LDLR mRNA in OVX + ISO group increased distinctively compared with OVX group and OVX + G group.


CONCLUSIONS
The level of mRNA of LDLR in liver decreas after ovariotormy and the soybean isoflavone may increase it by regulating the expression level of LDLR through transcription.
Ge
Xi-Zhen
XZ
Biochemical Engineering College of Beijing Union University, Beijing 100023, China.
Tian
Ping-Fang
PF
Lin
Qiang
Q
Huo
Qing
Q
D000818:Animals; D001161:Arteriosclerosis / Q000517:prevention & control; D008076:Cholesterol, HDL / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007529:Isoflavones / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D010052:Ovariectomy; D012333:RNA, Messenger / Q000096:biosynthesis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011973:Receptors, LDL / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013025:Glycine max / Q000737:chemistry*; D014280:Triglycerides / Q000097:blood
D008076:Cholesterol, HDL; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D012333:RNA, Messenger; D011973:Receptors, LDL; D014280:Triglycerides