Digestive plasticity in tadpoles of the Chilean giant frog (Caudiverbera caudiverbera): factorial effects of diet and temperature.
Anuran metamorphosis is one of the most spectacular processes in nature. Metamorphosis entails morphological transformations and extensive changes in feeding habits, such as transforming from an herbivore to a carnivore. This phenomenon is especially sensitive to environmental cues. We studied the phenotypic plasticity of intestinal morphology and enzyme activity in tadpoles of the Chilean giant frog Caudiverbera caudiverbera. We tested the effects of diet and temperature using a factorial design, which included a control of nontreated individuals. There was no significant effect of diet treatment (i.e., low- vs. high-quality diet) on any of the measured variables, including external morphology. We found significant effects of temperature on morphological traits. Temperature treatment also had significant effects on aminopeptidase-N and maltase activity. Both enzymes exhibited complex interactions with temperature along the intestine. Gut size varied significantly among temperatures, with intestines from warm-treated individuals smaller than the intestines from control and cold-treated tadpoles. Our findings suggest that phenotypic plasticity of intestinal morphology and physiology exists in larvae of this species, at least in response to temperature. However, we did not detect clear effects of diet or temperature on the timing of metamorphosis.
Castaneda
Luis E
LE
Instituto de Ecologia y Evolucion, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile.
Sabat
Pablo
P
Gonzalez
Sandra P
SP
Nespolo
Roberto F
RF
D000818:Animals; D004032:Diet; D004063:Digestion / Q000502:physiology*; D017753:Ecosystem; D007422:Intestines / Q000033:anatomy & histology / Q000201:enzymology; D007814:Larva / Q000502:physiology; D011898:Ranidae / Q000254:growth & development / Q000502:physiology*; D013696:Temperature; D013997:Time Factors