title: Murine polyomavirus-like particles induce maturation of bone marrow-derived dendritic cells and proliferation of T cells.
abstract: We analysed the effects of murine polyomavirus-like particles (PLPs) on bone marrow-derived dendritic cells (BMDCs) and T cells in vitro. BMDCs activated with PLPs up-regulated CD40, CD80, CD86 and major histocompatibility complex (MHC) class II surface markers and produced proinflammatory cytokines. Chimeric PLPs [expressing the ovalbumin (OVA)-peptides OVA(257-264) or OVA(323-339)], but not wildtype PLPs, activated OVA-specific CD8 T cells and OVA-specific CD4 T cells, respectively, indicating both MHC class I and II presentation of the peptides by antigen-presenting cells. Our results suggest that PLPs may be used as vaccine adjuvants priming dendritic cells to induce potent T cell responses.
authors.affiliation: Center for Infectious Diseases and Institute for Molecular Infection Biology, University of Wuerzburg, Wuerzburg, Germany. bickert@bni-hamburg.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Bickert
authors.forename: Gisela
authors.initials: G
authors.lastname: Wohlleben
authors.forename: Marc
authors.initials: M
authors.lastname: Brinkman
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Trujillo-Vargas
authors.forename: Claus
authors.initials: C
authors.lastname: Ruehland
authors.forename: Christian O A
authors.initials: CO
authors.lastname: Reiser
authors.forename: Juergen
authors.initials: J
authors.lastname: Hess
authors.forename: Klaus J
authors.initials: KJ
authors.lastname: Erb
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D017951:Antigen Presentation; D015703:Antigens, CD / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin; D011120:Polyomavirus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D015703:Antigens, CD; D016207:Cytokines; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D010047:Ovalbumin