title: Features of a newly cloned pig C1 esterase inhibitor.
abstract: The pig cDNA encoding C1 esterase inhibitor (C1-INH) was isolated and the homology of the sequence was compared with that from other animals. The structure of pig C1-INH contains a two disulfide bridge pattern identical to the human C1-INH. In the amino acid sequence of the first Cys-91 to the C-terminal end, the pigC1-INH has a 76.2% homology with the human protein, and the sequence of the reactive site is close to the human. A surface-bound form of pig and human C1-INH, pC1-INH-PI and hC1-INH, respectively, were next constructed. Stable Chinese hamster ovarian tumor (CHO) cell lines and pig endothelial cell (PEC) lines expressing these C1-INH-PI were prepared by transfection. The basic function and the species specificity of pCI-INH were then investigated using these transfectants. pC1-INH and hC1-INH have almost the same suppressive effect on pig, human, dog and rabbit sera in complement-dependent cell lysis, indicating little species specificity.
authors.affiliation: Division of Organ Transplantation, Department of Molecular Therapeutics, and Department of Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871.
authors.forename: Chizuko
authors.initials: C
authors.lastname: Kobayashi
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Matsunami
authors.forename: Takeshi
authors.initials: T
authors.lastname: Omori
authors.forename: Shino
authors.initials: S
authors.lastname: Nakatsu
authors.forename: Kengo
authors.initials: K
authors.lastname: Nakahata
authors.forename: Hengjie
authors.initials: H
authors.lastname: Xu
authors.forename: Ryota
authors.initials: R
authors.lastname: Shirakura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fukuzawa
authors.forename: Shuji
authors.initials: S
authors.lastname: Miyagawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D003174:Complement C1 Inactivator Proteins / Q000235:genetics*; D006224:Cricetinae; D003412:Cricetulus; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D003174:Complement C1 Inactivator Proteins; D017931:DNA Primers; D018076:DNA, Complementary