A transgenic zebrafish model of neutrophilic inflammation.
We have established an in vivo model for genetic analysis of the inflammatory response by generating a transgenic zebrafish line that expresses GFP under the neutrophil-specific myeloperoxidase promoter. We show that inflammation is induced after transection of the tail of zebrafish larvae and that this inflammation subsequently resolves over a similar time course to mammalian systems. Quantitative data can be generated from this model by counting of fluorescent cells or by digital image analysis. In addition, we show that the resolution of experimentally induced inflammation can be inhibited by the addition of a pancaspase inhibitor, zVD.fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model.
Renshaw
Stephen A
SA
MRC Centre for Developmental and Biomedical Genetics, Acadamic Unit of Respiratory Medicine, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom. s.a.renshaw@sheffield.ac.uk
Loynes
Catherine A
CA
Trushell
Daniel M I
DM
Elworthy
Stone
S
Ingham
Philip W
PW
Whyte
Moira K B
MK
D000818:Animals; D030801:Animals, Genetically Modified; D002452:Cell Count / Q000379:methods; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins; D007091:Image Processing, Computer-Assisted; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000473:pathology; D009504:Neutrophils / Q000276:immunology* / Q000473:pathology; D009195:Peroxidase / Q000235:genetics / Q000276:immunology; D011401:Promoter Regions, Genetic; D019076:Transgenes / Q000276:immunology; D014947:Wounds and Injuries / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D015027:Zebrafish / Q000235:genetics / Q000276:immunology*
D049452:Green Fluorescent Proteins; D009195:Peroxidase