title: Molecular diversity of disintegrin-like domains within metalloproteinase precursors of Bothrops jararaca.
abstract: Disintegrins are small peptides isolated from the venom of several snake families which act as integrin-antagonists or agonists, interacting with a variety of biological processes mediated by integrins. In this work we describe five new disintegrin-like domains within metalloproteinase precursor sequences, obtained from a Bothrops jararaca venom gland cDNA library. Among the new disintegrin-like domains, four were contained in PIII metalloproteinase precursors, with three of them presenting ECD-motifs and one presenting a new KCD-motif. Moreover, we found three disintegrin-like domains within PII metalloproteinase precursors. Two of them are similar to the already described disintegrins jarastatin and jararacin. The third molecule is unusual, presenting some typical PIII metalloproteinase characteristics but lacking the cysteine-rich domain being, thus, classified as a PII metalloproteinase. Only few reports presented molecules with these characteristics. Sequence analysis suggests that these molecules are intermediate steps between the more ancient PIII and the more recent PII metalloproteinases. We also investigated disintegrin N-terminus diversity in B. jararaca crude venom by purifying jarastatin and jararacin and analyzing them by mass spectrometry.
authors.affiliation: Departamento de Bioquímica, Universidade do Estado do Rio de Janeiro, CEP 20551-013 Rio de Janeiro, RJ, Brazil. dani_cidade@yahoo.com.br
authors.forename: Daniela A P
authors.initials: DA
authors.lastname: Cidade
authors.forename: Luciana S
authors.initials: LS
authors.lastname: Wermelinger
authors.forename: Gisele
authors.initials: G
authors.lastname: Lôbo-Hajdu
authors.forename: Alberto M R
authors.initials: AM
authors.lastname: Dávila
authors.forename: Cassian
authors.initials: C
authors.lastname: Bon
authors.forename: Russolina B
authors.initials: RB
authors.lastname: Zingali
authors.forename: Rodolpho M
authors.initials: RM
authors.lastname: Albano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017837:Bothrops / Q000502:physiology*; D020134:Catalytic Domain / Q000235:genetics; D003435:Crotalid Venoms / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D019483:Disintegrins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004792:Enzyme Precursors / Q000235:genetics* / Q000378:metabolism; D014644:Genetic Variation; D045726:Metalloproteases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013329:Structure-Activity Relationship
chemical_list: D003435:Crotalid Venoms; D018076:DNA, Complementary; D019483:Disintegrins; D004792:Enzyme Precursors; D045726:Metalloproteases