title: Assessment of a bovine viral diarrhea virus antigen capture ELISA and a microtiter virus isolation ELISA using pooled ear notch and serum samples.
abstract: To reduce the cost of whole herd screening for bovine viral diarrhea virus persistently infected animals, the sensitivity and specificity of an antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) and a microtiter virus isolation ELISA using saline from ear notch samples or pooled serum was determined. Pooled saline from ear notch samples, assayed by AC-ELISA, gave a sensitivity and specificity of 98% and 94%, respectively, for pools containing 2 samples and 72% and 100%, respectively, for pools of 5. The sensitivity of pooled ear notch or serum samples for bovine viral diarrhea virus detection by microtiter virus isolation (sensitivity < 5%) or serum samples for detection by AC-ELISA (sensitivity < 15%) is too low to be used for whole herd screening. Pooling saline from ear notch samples from 2 animals tested by AC-ELISA, however, could provide a less expensive, reliable method for whole herd screening for bovine viral diarrhea virus.
authors.affiliation: Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Cleveland
authors.forename: M D
authors.initials: MD
authors.lastname: Salman
authors.forename: Hana
authors.initials: H
authors.lastname: Van Campen
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000097:blood*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology* / Q000302:isolation & purification*; D004423:Ear / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012867:Skin / Q000821:virology*
chemical_list: D000956:Antigens, Viral