Pharmacological properties of rhamnose-rich polysaccharides, potential interest in age-dependent alterations of connectives tissues.
Rhamnose-rich oligo- and polysaccharides (RROPs) were tested for their potential pharmacological properties using human skin fibroblasts in serial cultures. The substances tested were shown to stimulate cell proliferation, decrease elastase-type activity, stimulate collagen biosynthesis, and protect hyaluronan against free radical mediated degradation. These reactions appear to be triggered by the mediation of a specific alpha-L-rhamnose recognizing lectin-site acting as a receptor, transmitting signals to the cell-interior. The rapid increase of intracellular free calcium after addition of RROP-1 and preliminary data using micro arrays appear also to confirm this contention.
Andrès
E
E
Laboratoire de recherches ophtalmologiques, Hôtel-Dieu, université Paris-V, 1, place Parvis-Notre-Dame, 75181 Paris cedex 04, France.
Molinari
J
J
Péterszegi
G
G
Mariko
B
B
Ruszova
E
E
Velebny
V
V
Faury
G
G
Robert
L
L
D000328:Adult; D002118:Calcium / Q000378:metabolism; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D009844:Oligosaccharides / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D012210:Rhamnose / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects; D012879:Skin Physiological Phenomena
D016166:Free Radical Scavengers; D009844:Oligosaccharides; D011134:Polysaccharides; D006820:Hyaluronic Acid; D003094:Collagen; D012210:Rhamnose; D002118:Calcium