[Contribution of dectin-1 to the recognition of fungal cell wall products and the activation of innate immune response].
1,3-Beta-glucans is a major cell wall component in fungi. Receptor molecules relating to innate immunity may recognize such cell wall products, and affect host defense systems. A beta-glucan receptor, dectin-1, is a C-type lectin and may contribute to the innate immune responses. To examine the role of dectin-1 in recognition of 1,3-beta-glucans and subsequent activation of intracellular signaling, the molecular characteristics of a carbohydrate recognition domain (CRD) of dectin-1 were investigated. The binding ability to beta-glucans was abolished by mutating two amino acid residues, Trp221 and His223, on the CRD. Dectin-1 increased TLR2-mediated NF-kappaB activation in response to zymosan. However, dectin-1 alone could not affect the activation pathway for NF-kappaB, nor did co-expression of dectin-1 mutant and TLR2 increase the NF-kappaB activation. These results suggest that dectin-1 may have a co-stimulatory effect on leukocyte activation in response to fungal infection.
Adachi
Yoshiyuki
Y
Laboratory for Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, Japan.
Ohno
Naohito
N
D002175:Candida / Q000276:immunology*; D002473:Cell Wall / Q000276:immunology; D037181:Lectins, C-Type; D008351:Mannans / Q000737:chemistry / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000276:immunology*; D047071:beta-Glucans / Q000276:immunology; D000099181:Dectin-1
D037181:Lectins, C-Type; D008351:Mannans; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D047071:beta-Glucans; D000099181:Dectin-1