[Microarray as a standard laboratory technique and as an unprecedented tool for understanding systems biology].
It is now possible to examine all the expressed genes present in a cell (transcriptome) simultaneously using microarray. Thus, microarrays have attracted tremendous interest among biologists. We have applied microarray technology to various studies regarding allergic diseases. Although we and others have discovered various novel molecules crucially involved in the pathogenesis of the disease, transcriptome assay is now expected as a tool for understanding overall molecule balancing, i.e., systems biology. Here we introduce examples of our trials to understand the functional roles of a certain cell type related to allergy as has been published in the web database. In the near future, we will be able to construct human inflammatory cell models in silico (in a computer) by analyzing integrative information regarding the genome, transcriptome and proteome of mast cells and can test our hypothesis without performing in vitro testing.
Saito
Hirohisa
H
Department of Allergy and Immunology, National Research Institute for Child Health & Development, Setagaya-ku, Tokyo.
D000818:Animals; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics*; D040081:Protein Array Analysis