Quantification of antihistamine acrivastine in plasma by solid-phase extraction and high-performance liquid chromatography.
An automated solid-phase extraction method was developed for the determination of the H1-antihistamine acrivastine in plasma samples. Acrivastine was analyzed at the wavelength of 254 nm using a reversed-phase HPLC assay. Both extraction procedure and analytical condition were optimized and validated for maximum recovery and resolution. The developed method was further applied to plasma samples collected from an in vivo pharmacokinetic study in rabbits. The assay was found to be simple, specific, accurate and reproducible.
Collins
Benjamin R
BR
Faculty of Pharmacy, University of Manitoba, 50 Sifton Road, Winnipeg, Man., Canada R3T 2N2.
Gu
Xiaochen
X
D000818:Animals; D019540:Area Under Curve; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003627:Data Interpretation, Statistical; D006207:Half-Life; D006634:Histamine H1 Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014311:Triprolidine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
D006634:Histamine H1 Antagonists; D014311:Triprolidine; C043648:acrivastine