Human postmortem brain tissue and 2-mm tissue microarrays.
The authors constructed tissue microarray (TMA) blocks from human postmortem brain including numerous core samples measuring 2 mm in diameter from various anatomic regions. These TMA sections were then processed using various stainings and pretreatment techniques to evaluate their properties. The loss of core samples ranged from 2% to 100% and was significantly influenced by the type of glass slide used; it was lowest (2-8%) with SuperFrost Plus slides. The losses were not significantly altered when applying the most demanding pretreatment procedures or using human brain tissue with a long postmortem delay. A slight influence on the quality and the repeatability of some of the IHC stainings was seen by the postmortem delay, by the brain region, or by the glass slide used. One special feature of the constructed brain TMA block including many anatomic brain regions is that persons who lack skills in neuroanatomy can identify various brain structures simply by following the x-y coordinates. Thus, the applications of this brain TMA block in neurologic research by scientists with different skill bases remain to be determined.
Kauppinen
Tarja
T
Department of Neuroscience and Neurology, University of Kuopio, Kuopio, Finland.
Martikainen
Paula
P
Alafuzoff
Irina
I
D001344:Autopsy; D001921:Brain / Q000473:pathology*; D016591:Histocytological Preparation Techniques / Q000379:methods / Q000592:standards*; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards*; D011180:Postmortem Changes; D013048:Specimen Handling; D013194:Staining and Labeling / Q000379:methods* / Q000592:standards; D046888:Tissue Array Analysis / Q000379:methods* / Q000592:standards; D016610:Tissue Embedding