Comparative study of cytotoxicity of Aeromonas spp. on four different cell lines.
In vitro cytotoxicity is an important virulence property of motile mesophilic Aeromonas species. Cell-free supernatant prepared from 55 Aeromonas isolates including one A. hydrophila type strain (MTCC 646) were examined for their cytotoxic potential on four different cell lines (Vero, BHK-21, MDBK, B 95a). Results of the study revealed cytotoxic potential in 92.72% of the isolates. Analysis of data exposed significant variation among isolates in respect of their cytotoxicity. Vero cells proved to be most sensitive to aeromonal toxins and B 95a cells showed significantly (P<0.01) lower response compared to other cell lines. Sensitivities of BHK-21 and MDBK cell lines were in between Vero and B 95a.
Ghatak
S
S
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, India.
Agarwal
R K
RK
Bhilegaonkar
K N
KN
D000333:Aeromonas / Q000276:immunology / Q000378:metabolism* / Q000472:pathogenicity; D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology / Q000378:metabolism*; D002144:Callithrix; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans
D001427:Bacterial Toxins