Absolute configuration of hydroxycitric acid produced by microorganisms.
Optical resolution for (2S,3R) and (2R,3S)-hydroxycitric acid (HCA) enantiomers was developed using chiral column chromatography. HCA from Bacillus megaterium G45C and Streptomyces sp. U121, newly isolated in our previous study, was analyzed to determine the absolute configuration. These results indicate that both strains generate optically pure (2S,3R)-hibiscus type HCA enantiomer.
Hida
Hiroyuki
H
Department of Applied Biological Science, Faculty of Life Science and Biotechnology, Fukuyama University, Hiroshima, Japan. yamada@fubac.fukuyama-u.ac.jp
Yamada
Takashi
T
Yamada
Yasuhiro
Y
D001410:Bacillus megaterium / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002951:Citrates / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008968:Molecular Conformation; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013302:Streptomyces / Q000378:metabolism*; D013997:Time Factors
D002951:Citrates; C007999:hydroxycitric acid