title: Establishment of a murine model of bone invasion by oral squamous cell carcinoma.
abstract: This study examines the establishment an animal model of bone invasion by oral squamous cell carcinoma to clarify the mechanisms of osteoclast-mediated bone invasion. C(3)H/HeN mice were inoculated with SCCVII cells into the masseter region. At the end of week 3, all surviving mice were sacrificed and analyzed by three-dimensional imaging using micro-computed tomography, histopathological observation using Hematoxylin-Eosin staining and Tartrate-Resistant Acid Phosphatase staining, and confirmation of mRNA expression of the osteoclast-related cytokines IL-6, TNF-alpha, and PTHrP. SCCVII cells rapidly multiplied in the masseter muscle of the mice. Bone invasion was evident only in the SCCVII transplanted group on micro-computed tomography. The histopathologic findings obtained with H-E and TRAP staining indicated that the tumor cells in the mandible of all animals of the SCCVII transplanted group exhibited funicular invasion and presented a serrated pattern of bone resorption. The mRNA expression of IL-6, PTHrP, and TNF-alpha increased as the control decreased. SCCVII cells were highly invasive into mandibular bone in C(3)H/HeN mice. This model was similar to the invasion of human oral cancer into maxillary and mandibular bone. Our mandibular invasion model may provide a powerful new modality for the diagnosis and treatment of oral cancer with bone invasion.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tokyo Dental College, 1-2-2 Masago, Mihamaku, Chiba 261-8502, Japan. tanomura@tdc.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nomura
authors.forename: Takahiko
authors.initials: T
authors.lastname: Shibahara
authors.forename: Akira
authors.initials: A
authors.lastname: Katakura
authors.forename: Shizuka
authors.initials: S
authors.lastname: Matsubara
authors.forename: Nobuo
authors.initials: N
authors.lastname: Takano
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000473:pathology / Q000503:physiopathology; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000503:physiopathology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D007568:Jaw / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D008334:Mandible / Q000473:pathology / Q000503:physiopathology; D008437:Maxilla / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D008809:Mice, Inbred C3H; D009062:Mouth Neoplasms / Q000473:pathology* / Q000503:physiopathology; D009361:Neoplasm Invasiveness; D010010:Osteoclasts / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D014057:Tomography, X-Ray Computed / Q000379:methods; D014407:Tumor Cells, Cultured
chemical_list: D016207:Cytokines; D012333:RNA, Messenger