Separation of doxorubicin and doxorubicinol by cyclodextrin-modified micellar electrokinetic capillary chromatography.
Doxorubicinol (DOXol) is a human metabolite of the chemotherapy agent doxorubicin (DOX), and is associated with dose-dependent cardiotoxicity and decreased drug efficacy. Due to the structural similarities and equal molecular charges of DOXol and DOX, their electrophoretic separation is commonly ineffective. A method for separating and detecting DOX and DOXol, as well as two DOXol enantiomers, was established using cyclodextrin-modified micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. Differential DOXol production was detected in a DOX-sensitive and resistant pair of cell lines, with a 0.08 +/- 0.01 fmol limit of detection.
Eder
Angela R
AR
Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Chen
Jennifer S
JS
Arriaga
Edgar A
EA
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry*; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D006801:Humans; D007834:Lasers; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D014407:Tumor Cells, Cultured
D003505:Cyclodextrins; D004317:Doxorubicin; C010013:adriamycinol