title: The effects of tumor necrosis factor-alpha, interleukin-1beta, interleukin-6, and transforming growth factor-beta1 on pulp fibroblast mediated collagen degradation.
abstract: Dental pulp destruction is believed to be regulated, in part, by the matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs). Cytokines are believed to be important in the pathogenesis of pulpitis. This study examined the effects that TNF-alpha, IL-1beta, IL-6, and TGF-beta1 have on the collagen degradation mediated by pulp fibroblasts utilizing a cell-mediated collagen degradation assay. Reverse transcriptase-polymerase chain reaction, Western blot analyses, and zymography were utilized to examine multiple MMPs and TIMPs. The collagen degradation mediated by these cells was stimulated by these cytokines. TNF-alpha, IL-1beta, and IL-6 increased the mRNA and/or protein expression of MMP-1, MMP-2, and MMP-3. TGF-beta1 decreased MMP-1 mRNA expression, while only slightly affecting the MMP-2 and MMP-3 mRNA and/or protein. These cytokines did not affect the expression of TIMP-1 or TIMP-2. These results suggest that these cytokines affect pulp destruction, in part, by differentially regulating the MMPs and TIMPs.
authors.affiliation: Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, IN 46202, USA.
authors.forename: Kessiri
authors.initials: K
authors.lastname: Wisithphrom
authors.forename: L Jack
authors.initials: LJ
authors.lastname: Windsor
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002478:Cells, Cultured; D016207:Cytokines / Q000502:physiology*; D003782:Dental Pulp / Q000166:cytology / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D024022:Fibrillar Collagens / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology; D015850:Interleukin-6 / Q000502:physiology; D020782:Matrix Metalloproteinases / Q000096:biosynthesis*; D011671:Pulpitis / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
chemical_list: D016207:Cytokines; D024022:Fibrillar Collagens; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D020782:Matrix Metalloproteinases