[Analysis of anisodine and its metabolites in rat plasma by liquid chromatography-tandem mass spectrometry].
OBJECTIVE
To identify anisodine and its metabolites in rat plasma after ingestion of anisodine by combining liquid chromatography and tandem mass spectrometry (LC-MS(n)).


METHODS
Plasma samples from rats after a single orally administration of 20 mg anisodine were added with methanol to precipitate protein. Then, it was analyzed by LC-MS(n). Identification and structural elucidation of the metabolites were performed by comparing their changes in molecular masses, retention-times and full scan MS(n) spectra with those of the parent drug and blank plasma.


RESULTS
The results revealed that the parent drug and its four metabolites (norscopine, scopine, hydroxyanisodine, N-oxide anisodine) existed in rat plasma.


CONCLUSIONS
This method is sensitive, rapid, simple, and it is suitable for the rapid identification of drug and its metabolits.
Chen
Huai-Xia
HX
Hubei Province Key Lab of Bio-Technology of Traditional Chinese Medicine, Hubei University, Wuhan 430062, China.
Du
Peng
P
Han
Feng-Mei
FM
Chen
Yong
Y
D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012602:Scopolamine Derivatives / Q000302:isolation & purification / Q000378:metabolism*; D012680:Sensitivity and Specificity; D019657:Solanaceae / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods
D012602:Scopolamine Derivatives; C012183:anisodine