Memory-enhancing effect of a supercritical carbon dioxide fluid extract of the needles of Abies koreana on scopolamine-induced amnesia in mice.
Abies koreana Wilson (A. koreana) is a shrub or broadly pyramidal evergreen tree endemic in the mountainous regions of South Korea. We obtained the essential oil (EO) from alpine needle leaves of A. koreana by the supercritical fluid extraction (SFE) method. EO was analyzed by gas chromatography-mass spectrometry (GC-MS), and 68 compounds were identified constituting 95.66% of the oil. The major components were elemol (11.17%), terpinen-4-ol (9.77%), sabinene (8.86%), 10(15)-cadien-4-ol (7.16%), alpha-terpineol (6.13%), alpha-pinene (6.07%) and gamma-terpinene (4.71%). To investigate the memory-enhancing effects, we conducted a passive avoidance test using a scopolamine (1 mg/kg, ip)-induced amnesia mouse model. A peritoneal injection of EO from A. koreana (100 mg/kg) showed a memory enhancing effect of 72.7% compared with the control. These results suggest that EO of A. koreana may be a useful therapeutic agent against such amnesia-inducing diseases as Alzheimer and vascular dementia.
Kim
Kanghyun
K
Department of Oriental Pharmacy, College of Pharmacy, Woosuk University, Chonbuk, Korea.
Bu
Youngmin
Y
Jeong
Seungil
S
Lim
Jongpil
J
Kwon
Youngan
Y
Cha
Dong Seok
DS
Kim
Jinmo
J
Jeon
Sora
S
Eun
Jaesoon
J
Jeon
Hoon
H
D028202:Abies / Q000737:chemistry*; D000647:Amnesia / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D002245:Carbon Dioxide / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002849:Chromatography, Gas; D025924:Chromatography, Supercritical Fluid; D004195:Disease Models, Animal; D008401:Gas Chromatography-Mass Spectrometry; D007274:Injections, Intraperitoneal; D008297:Male; D008568:Memory / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D012601:Scopolamine; D012680:Sensitivity and Specificity
D009822:Oils, Volatile; D002245:Carbon Dioxide; D012601:Scopolamine