title: A biofilm enhanced miniature microbial fuel cell using Shewanella oneidensis DSP10 and oxygen reduction cathodes.
abstract: A miniature-microbial fuel cell (mini-MFC, chamber volume: 1.2 mL) was used to monitor biofilm development from a pure culture of Shewanella oneidensis DSP10 on graphite felt (GF) under minimal nutrient conditions. ESEM evidence of biofilm formation on GF is supported by substantial power density (per device cross-section) from the mini-MFC when using an acellular minimal media anolyte (1500 mW/m2). These experiments demonstrate that power density per volume for a biofilm flow reactor MFC should be calculated using the anode chamber volume alone (250W/m3), rather than with the full anolyte volume. Two oxygen reduction cathodes (uncoated GF or a Pt/vulcanized carbon coating on GF) were also compared to a cathode using uncoated GF and a 50mM ferricyanide catholyte solution. The Pt/C-GF (2-4% Pt by mass) electrodes with liquid cultures of DSP10 produced one order of magnitude larger power density (150W/m3) than bare graphite felt (12W/m3) in this design. These advances are some of the required modifications to enable the mini-MFC to be used in real-time, long-term environmental power generating situations.
authors.affiliation: Chemistry Division, Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, DC 20375, USA.
authors.forename: Justin C
authors.initials: JC
authors.lastname: Biffinger
authors.forename: Jeremy
authors.initials: J
authors.lastname: Pietron
authors.forename: Ricky
authors.initials: R
authors.lastname: Ray
authors.forename: Brenda
authors.initials: B
authors.lastname: Little
authors.forename: Bradley R
authors.initials: BR
authors.lastname: Ringeisen
mesh_terms: D001674:Bioelectric Energy Sources; D018441:Biofilms; D004566:Electrodes; D008904:Miniaturization; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D020592:Shewanella / Q000502:physiology*
chemical_list: D010100:Oxygen