title: Early apoptosis in CD34+ cells as a potential heterogeneity in quality of cryopreserved umbilical cord blood.
abstract: The increased use of umbilical cord blood (UCB) raises issues regarding the quality of cryopreserved UCB. This study investigated whether early apoptosis of CD34+ cells is part of the functional heterogeneity of cryopreserved UCB. Annexin V binding of CD34+ PI(-) cells showed wide variations in both fresh and cryopreserved UCBs, with greater variation among units frozen for > 5 years. Xenotransplantation of sorted cells into non-obese diabetic severe combined immunodeficient mice demonstrated that the Annexin V assay identified most repopulating activities in UCB units. Thus, early apoptosis of CD34+ cells could influence the outcome of transplantation using cryopreserved UCB.
authors.affiliation: Catholic Cell Therapy Centre and Department of Cellular Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Jae-Seung
authors.initials: JS
authors.lastname: Shim
authors.forename: Bin
authors.initials: B
authors.lastname: Cho
authors.forename: Myungshin
authors.initials: M
authors.lastname: Kim
authors.forename: Gyeong-Sin
authors.initials: GS
authors.lastname: Park
authors.forename: Jong-Chul
authors.initials: JC
authors.lastname: Shin
authors.forename: Hong-Kyu
authors.initials: HK
authors.lastname: Hwang
authors.forename: Tae-Gyu
authors.initials: TG
authors.lastname: Kim
authors.forename: Il-Hoan
authors.initials: IH
authors.lastname: Oh
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D018952:Antigens, CD34 / Q000097:blood*; D017209:Apoptosis; D001771:Blood Banks; D001793:Blood Preservation / Q000379:methods; D036101:Cord Blood Stem Cell Transplantation; D015925:Cryopreservation; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D006085:Graft Survival; D006801:Humans; D007231:Infant, Newborn; D051379:Mice; D016688:Mice, Inbred NOD; D014183:Transplantation, Heterologous
chemical_list: D017304:Annexin A5; D018952:Antigens, CD34