title: Transporters expressed during grape berry (Vitis vinifera L.) development are associated with an increase in berry size and berry potassium accumulation.
abstract: Potassium accumulation is essential for grapevine (Vitis vinifera L.) growth and development, but excessive levels in berries at harvest may reduce wine quality particularly for red wines. In addition to decreasing the free acid levels, potassium also combines with tartaric acid to form largely insoluble potassium bitartrate. This precipitates during winemaking and storage, resulting in an increase in wine pH that is associated with negative impacts on wine colour, flavour, and microbiological stability. For these reasons, a better understanding of potassium transport and accumulation within the vine and berries is important for producing fruit with improved winemaking characteristics. Here two genes encoding KUP/KT/HAK-type potassium transporters that are expressed in grape berries are described. Their function as potassium transporters was demonstrated by complementation of an Escherichia coli mutant. The two transporters are expressed most highly in the berry skin during the first phase of berry development (pre-veraison), with similar patterns in two grapevine varieties. The timing and location of expression of these transporters are consistent with an involvement in potassium accumulation in grape berries.
authors.affiliation: CSIRO Plant Industry and Cooperative Research Centre for Viticulture, PO Box 350, Glen Osmond, SA 5064, Australia. Christopher.Davies@csiro.au
authors.forename: Christopher
authors.initials: C
authors.lastname: Davies
authors.forename: Ryoung
authors.initials: R
authors.lastname: Shin
authors.forename: Weihong
authors.initials: W
authors.lastname: Liu
authors.forename: Mark R
authors.initials: MR
authors.lastname: Thomas
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Schachtman
mesh_terms: D015139:Blotting, Southern; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018076:DNA, Complementary / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005638:Fruit / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism; D005816:Genetic Complementation Test; D017136:Ion Transport; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011188:Potassium / Q000737:chemistry / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D027843:Vitis / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism
chemical_list: D027682:Cation Transport Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; D011188:Potassium