Molluscicidal effect of three monoterpenes oils on schistosomiasis and fascioliasis vector snails in Egypt.
Thymol, Linalool and Eugenol showed considerable molluscicidal effect against Biomphalaria alexandrina, Bulinus truncatus and Lymnneae natalensis. The thymol was the potent one at least LC50 and LC90) followed by euganol then linalool. L. natalensis were more sensitive to these compounds followed by B. truncatus and then B. alexandrina. The LC50 & LC90) of thymol were 22 and 34 ppm against B. alexaldrina, 20 and 30 ppm for B. truncatuts and 18 and 29 ppm for L. nalalensis. These values were higher with Eugenol, 28 and 48 ppm for B. alexuadrina, 24 and 44 ppm for B. truncatus and 22 and 40 ppm for L. natalensis. Linalool showed highest values of LCs5 and LC90 against B. alexandrina, 34 and 56 ppm, against B. truncatus 30 and 52 ppm and for L. natalensis 28 and 48 ppm, respectively. Maintaining of B. alexandrina at LC10 of Thymol for one week induced an inhibitory effect in the level of some enzymes (AchE, SDH). It led to increase in the activity of other enzymes (ACP, ALP & G-6-PD). Acetylcholine-sterase activity (AchE) of treated B. alexandrina was significantly reduced by 45.9% when compared to control. The results showed a significant decrease in succinate dehydrogenase activity (SDH) by 46.4% together with a concomitant increase in glucose-6-phosphate dehydrogenase activity level (G-6-PD) by 47.5% in comparison with control. The activities of acid phosphatase and alkaline phosphatase enzymes were found to be higher in the treated snails than in control ones. The percentage increases were 47.2% & 73.2% respectively. The results also showed an elevation in the hemolymph glucose content of treated snails by 51.9% while the tissue glycogen content was reduced by 48.1%. The infection of B. alexandrina with S. mansoni miracidia was greatly reduced by thymol LC10 (sublethal dose). The infection rate reduction was 43.1%. The treated snails' prepatent period was prolonged (34.2 +/- 3.3 days) compared to control (28.4 +/- 1.2 days). A highly significant reduction of total cercarial production per snail occurred in experimental snails as compared to control.
el-Din
Ahmed T Sharaf
AT
Department of Environmental and Medical Malacolgy, Theodor Bilharz Research Institute, P.O. Box 30 Imbaba, Giza, Egypt.
D000080462:Acyclic Monoterpenes; D000818:Animals; D004199:Disease Vectors; D004534:Egypt; D005054:Eugenol / Q000494:pharmacology; D005211:Fascioliasis / Q000635:transmission; D007928:Lethal Dose 50; D008975:Molluscacides / Q000494:pharmacology*; D039821:Monoterpenes / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology*; D012552:Schistosomiasis / Q000635:transmission; D012908:Snails / Q000187:drug effects* / Q000254:growth & development* / Q000469:parasitology; D013943:Thymol / Q000494:pharmacology; D013997:Time Factors; D016896:Treatment Outcome
D000080462:Acyclic Monoterpenes; D008975:Molluscacides; D039821:Monoterpenes; D010938:Plant Oils; D013943:Thymol; D005054:Eugenol; C018584:linalool