Isolation and characterization of full-length recombinant cattle PrPC protein.
Full-length Bos taurus PrPC protein was obtained in the eu- and prokaryotic expression systems. Immunoblotting and indirect enzyme immunoassay demonstrated high specificity and antigenic activity of full-length proteins in the reactions with monoclonal antibodies (anti-SAF-32 and VRQ-84). Membrane location of recombinant PrPC protein in insect cells was shown by immunofluorescent analysis.
Kal'nov
S L
SL
D I Ivanovsky Institute of Virology, Russian Academy of Medical Sciences. kalnov@narvac.com
Grigor'ev
V B
VB
Alekseev
K P
KP
Vlasova
A N
AN
Gibadulin
R A
RA
Pokidyshev
A N
AN
Balandina
M V
MV
Tsibezov
V V
VV
Verkhovskii
O A
OA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000032:analysis; D016367:Baculoviridae / Q000235:genetics; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry; D018621:PrPC Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D018621:PrPC Proteins; D011994:Recombinant Proteins