A novel acoustic evaluation system of scratching in mouse dermatitis: rapid and specific detection of invisibly rapid scratch in an atopic dermatitis model mouse.
Scratching is an essential and a skin specific behavior induced by itching, which is a common symptom of atopic dermatitis (AD) and other types of dermatitis. Itching sensation and scratching are closely associated and thus scratching times are currently used for evaluating itching in animal models. However, objective measuring systems of scratching to assess the grade of dermatitis and the effectiveness of anti-pruritic drugs in animal dermatitis models are lacking. To investigate a quantitative evaluation system for itching, we have developed a novel acoustic scratching counting system and compared its accuracy with time-consuming slow-motion video recording system. We have also objectively evaluated the efficacy of an antihistamine using this novel system. Scratching behavior of an AD model mouse (K14 driven IL-18 transgenic mouse) was recorded visually and acoustically. Specific scratching sound produced by mice was recorded and counted using a software we have developed, and the results obtained using our acoustic system were not statistically different from data obtained using slow motion video system. Surprisingly, mice scratched more than 10 times/second, which was invisibly rapid motion and revealed inaccuracy of conventional hand counting system. Results were identical to that of measured by 10 times time costing slow-motion video analysis. The antihistamine is clearly effective for suppression of scratching as demonstrated using this objective and accurate method. This novel motion analysis system will open a window for physiological and pathological analysis for animal models and development of anti-pruritic drugs.
Umeda
Koji
K
Department of Dermatology, Mie University, Graduate School of Medicine, 2-174, Edobashi, Tsu, Mie 514-8507, Japan.
Noro
Yuichi
Y
Murakami
Takaaki
T
Tokime
Kazuya
K
Sugisaki
Hitomi
H
Yamanaka
Keiichi
K
Kurokawa
Ichiro
I
Kuno
Kazuhiro
K
Tsutsui
Hiroko
H
Nakanishi
Kenji
K
Mizutani
Hitoshi
H
D000818:Animals; D003876:Dermatitis, Atopic / Q000503:physiopathology*; D004195:Disease Models, Animal; D006614:Hindlimb; D006801:Humans; D053547:Keratin-14 / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D015588:Observer Variation; D011537:Pruritus / Q000503:physiopathology*; D014743:Videotape Recording
C507540:KRT14 protein, human; D053547:Keratin-14