Study on the decomposition of 2-chloroethylnitro- sosulfamides (CENS) in serum using HPLC on-line solid phase extraction.
In this paper we have investigated the kinetic decomposition of 2-chloroethylnitrososulfamides (CENS) in fetal calf serum. The study was monitored by on-line solid phase extraction linked to RP-LC-MS. We demonstrated that CENS are less stable in fetal calf serum than in aqueous buffer at pH 7.4 and 37 degrees C. Moreover, we have shown that partition coefficient of CENS can be correlated to the kinetics decomposition, and we observe that the stability is better for lipophilic CENS. The different metabolites after decomposition have been tentatively identified by RP-HPLC-MS. This study indicates the formation of several metabolites resulting from a mechanism of decomposition more complex than in aqueous phosphate buffer, nevertheless, leading to the same main products.
Seridi
Achour
A
Laboratoire de Chimie Biomoléculaire UMR 5032, Université Montpellier II, ENSCM, Montpellier, France.
Winum
Jean-Yves
JY
Kadri
Mekki
M
Abdaoui
Mohamed
M
Montero
Jean-Louis
JL
D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D005312:Fetal Blood / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D009603:Nitroso Compounds / Q000737:chemistry* / Q000378:metabolism; D009862:Online Systems; D013450:Sulfones / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology
D009603:Nitroso Compounds; D013450:Sulfones