Exogenous polyamines enhance copper tolerance of Nymphoides peltatum.
The protective effects of polyamines (PAs) against copper (Cu) toxicity were investigated in the leaves of Nymphoides peltatum. Cu treatment increased the putrescine (Put) level and lowered spermidine (Spd) and spermine (Spm) levels, thereby reducing the (Spd+Spm)/Put ratio in leaves. Exogenous application of Spd or Spm markedly reversed these Cu-induced effects for all three PAs and partially restored the (Spd+Spm)/Put ratio in leaves. It also significantly enhanced the level of proline, retarded the loss of soluble protein, decreased the rate of O2*- generation and H2O2 content, and prevented Cu-induced lipid peroxidation. Furthermore, exogenous Spd and Spm reduced the accumulation of Cu and effectively maintained the balance of nutrient elements in plant leaves under Cu stress. These results suggest that exogenous application of Spd or Spm can enhance the tolerance of N. peltatum to Cu by increasing the levels of endogenous Spd and Spm as well as the (Spd+Spm)/Put ratio.
Wang
Xue
X
Laboratory of Plant Cell and Molecular Biology, College of Life Science, Nanjing Normal University, Nanjing 210097, China. xue_wang@163.com
Shi
Guoxin
G
Xu
Qinsong
Q
Hu
Jinzhao
J
D019659:Asteraceae / Q000187:drug effects / Q000502:physiology*; D003300:Copper / Q000633:toxicity*; D015227:Lipid Peroxidation; D008670:Metals / Q000633:toxicity; D010758:Phosphorus / Q000494:pharmacology; D011073:Polyamines / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
D008670:Metals; D011073:Polyamines; D013481:Superoxides; D010758:Phosphorus; D003300:Copper; D012964:Sodium; D011188:Potassium