title: Denopamine stimulates alveolar fluid clearance via cystic fibrosis transmembrane conductance regulator in rat lungs.
abstract: OBJECTIVE
  The objective of this study was to test the hypothesis that cystic fibrosis transmembrane conductance regulator (CFTR) plays a role in beta(1)-adrenergic agonist-stimulated alveolar fluid clearance.
  
  
  METHODS
  Isotonic 5% albumin solutions containing different pharmacological agents were instilled into the alveolar spaces of the isolated rat lungs. The lungs were inflated with 100% oxygen at an airway pressure of 7 cm H(2)O and placed in a humidified incubator at 37 degrees C. Alveolar fluid clearance was estimated by the progressive increase in the albumin concentration over 1 h. To test the hypothesis, we determined whether CFTR Cl(-) channel inhibitors (glibenclamide and CFTR(inh)-172) inhibited the effect of denopamine, a beta(1)-adrenergic agonist, on stimulation of alveolar fluid clearance in the isolated rat lungs.
  
  
  RESULTS
  Denopamine increased alveolar fluid clearance in a dose-dependent manner. Atenolol, a beta(1)-adrenergic antagonist, abolished the effects of denopamine on stimulation of alveolar fluid clearance. Although glibenclamide alone or CFTR(inh)-172 alone did not change basal alveolar fluid clearance, these CFTR inhibitors inhibited the effect of denopamine on alveolar fluid clearance.
  
  
  CONCLUSIONS
  CFTR plays a role in beta(1)-adrenergic agonist-stimulated alveolar fluid clearance in rat lungs.
authors.affiliation: Thoracic Surgery, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan.
authors.forename: Xiu
authors.initials: X
authors.lastname: Gu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Sumiko
authors.initials: S
authors.lastname: Maeda
authors.forename: Makoto
authors.initials: M
authors.lastname: Sugita
authors.forename: Motoyasu
authors.initials: M
authors.lastname: Sagawa
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Toga
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sakuma
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000187:drug effects*; D004983:Ethanolamines / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D011650:Pulmonary Alveoli / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000318:Adrenergic beta-Agonists; D004983:Ethanolamines; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; C037293:denopamine