A panel of optimized primers and positive-control DNAs for PCR detection of common biological contaminants in mouse cell lines and tissue samples.
PCR-based testing for infectious agents in mouse cell lines and tissues has recently been developed as an alternative to the traditional MAP test. One drawback to currently available PCR-based assays is the lack of appropriate positive controls for PCR detection of the infectious agents. When negative samples are the norm and positive controls are absent, it is very difficult to feel confident detecting infectious agents. To alleviate this problem, the authors developed a panel of primers and positive-control DNA plasmids that enable rapid testing of biological samples, such as cell lines, tissues, or animal sera, for presence of the infectious agents most damaging to mouse colonies.
Ayral
Anne-Marie
AM
Medical Research Council, Harwell, Didcot OX11 0RD UK.
Clarkson
Sarah
S
Cheeseman
Michael
M
Wells
Sara
S
Dear
T Neil
TN
D000818:Animals; D000830:Animals, Laboratory; D001419:Bacteria / Q000235:genetics; D001424:Bacterial Infections / Q000175:diagnosis / Q000382:microbiology*; D002460:Cell Line; D017931:DNA Primers; D051379:Mice; D010957:Plasmids; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis / Q000821:virology*; D014780:Viruses / Q000235:genetics
D017931:DNA Primers