Simultaneous determination of streptomycin and oxytetracycline in agricultural antimicrobials by CZE after an experimental design.
A capillary zone electrophoresis (CZE) method was developed and validated for the simultaneous determination of both streptomycin (STP) and oxytetracycline (OTC) in bactericidal products to be used in agriculture. Using fused-silica capillaries, the influence of the electrolyte composition, pH and concentration, as well as temperature and applied voltage were investigated using a central composite design to optimize the method. The optimized electrophoretic conditions were as follows: 0.10 M sodium phosphate, pH 2.5, 7.0 kV and 20.0 degrees C. The method was validated for STP and OTC determination in agricultural formulations through the following performance criteria: linearity and linear range, sensitivity, selectivity, intra-day and inter-day precision, detectability, accuracy and ruggedness. This optimized CZE-method for the identification and quantification of STP and OTC is a potential alternative method to the HPLC methods described by the US Pharmacopeia, with the advantage that the same method could be used for the simultaneous determination of these different antibiotics.
Maia
Patrícia Penido
PP
Department of Food Science, State University of Campinas, P.O. Box 6121, 13084-971 Campinas, SP, Brazil.
Amaya-Farfán
Jaime
J
Rath
Susanne
S
Reyes
Felix Guillermo Reyes
FG
D016573:Agrochemicals / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010118:Oxytetracycline / Q000032:analysis*; D015203:Reproducibility of Results; D013307:Streptomycin / Q000032:analysis*; D013696:Temperature
D016573:Agrochemicals; D000890:Anti-Infective Agents; D010118:Oxytetracycline; D013307:Streptomycin