Early apoptosis in CD34+ cells as a potential heterogeneity in quality of cryopreserved umbilical cord blood.
The increased use of umbilical cord blood (UCB) raises issues regarding the quality of cryopreserved UCB. This study investigated whether early apoptosis of CD34+ cells is part of the functional heterogeneity of cryopreserved UCB. Annexin V binding of CD34+ PI(-) cells showed wide variations in both fresh and cryopreserved UCBs, with greater variation among units frozen for > 5 years. Xenotransplantation of sorted cells into non-obese diabetic severe combined immunodeficient mice demonstrated that the Annexin V assay identified most repopulating activities in UCB units. Thus, early apoptosis of CD34+ cells could influence the outcome of transplantation using cryopreserved UCB.
Shim
Jae-Seung
JS
Catholic Cell Therapy Centre and Department of Cellular Medicine, The Catholic University of Korea, Seoul, Korea.
Cho
Bin
B
Kim
Myungshin
M
Park
Gyeong-Sin
GS
Shin
Jong-Chul
JC
Hwang
Hong-Kyu
HK
Kim
Tae-Gyu
TG
Oh
Il-Hoan
IH
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D018952:Antigens, CD34 / Q000097:blood*; D017209:Apoptosis; D001771:Blood Banks; D001793:Blood Preservation / Q000379:methods; D036101:Cord Blood Stem Cell Transplantation; D015925:Cryopreservation; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D006085:Graft Survival; D006801:Humans; D007231:Infant, Newborn; D051379:Mice; D016688:Mice, Inbred NOD; D014183:Transplantation, Heterologous
D017304:Annexin A5; D018952:Antigens, CD34