title: Separation and identification of compounds in Adinandra nitida by comprehensive two-dimensional liquid chromatography coupled to atmospheric pressure chemical ionization source ion trap tandem mass spectrometry.
abstract: A comprehensive two-dimensional liquid chromatographic (2D-LC) separation system based on the combination of a CN column and a Merck Chromolith Flash reversed-phase column was developed for the separation of components in Adinandra nitida, one type of traditional Chinese medicine (TCM). The two dimensions were connected by a ten-port, dual-position valve controlled automatically by software written in-house. The effluents were detected by both ultraviolet and atmospheric pressure chemical ionization source ion trap tandem mass spectrometry (MS). The calculated peak capacity of the 2D-LC-MS/MS system was above 1240. More than 57 components were resolved in the methanol extract from Adinandra nitida leaves, and five of these were identified based on their relative retention times, molecular weights and MS/MS spectra.
authors.affiliation: National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Dingyin
authors.initials: D
authors.lastname: Tao
authors.forename: Jicheng
authors.initials: J
authors.lastname: Duan
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liang
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.forename: Yushu
authors.initials: Y
authors.lastname: Huo
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D019684:Magnoliopsida / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011312:Pressure