The emergence of yeast lipidomics.
The emerging field of lipidomics, driven by technological advances in lipid analysis, provides greatly enhanced opportunities to characterize, on a quantitative or semi-quantitative level, the entire spectrum of lipids, or lipidome, in specific cell types. When combined with advances in other high throughput technologies in genomics and proteomics, lipidomics offers the opportunity to analyze the unique roles of specific lipids in complex cellular processes such as signaling and membrane trafficking. The yeast system offers many advantages for such studies, including the relative simplicity of its lipidome as compared to mammalian cells, the relatively high proportion of structural and regulatory genes of lipid metabolism which have been assigned and the excellent tools for molecular genetic analysis that yeast affords. The current state of application of lipidomic approaches in yeast and the advantages and disadvantages of yeast for such studies are discussed in this report.
Gaspar
Maria L
ML
Department of Molecular Biology and Genetics, Cornell University, 260 Roberts Hall, Ithaca, NY 14853, USA.
Aregullin
Manuel A
MA
Jesch
Stephen A
SA
Nunez
Lilia R
LR
Villa-García
Manuel
M
Henry
Susan A
SA
D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D007294:Inositol / Q000378:metabolism; D007425:Intracellular Membranes / Q000737:chemistry; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000502:physiology*; D008563:Membrane Lipids / Q000737:chemistry; D010712:Phosphatidic Acids / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic; D015003:Yeasts / Q000502:physiology*
D008055:Lipids; D008563:Membrane Lipids; D010712:Phosphatidic Acids; D007294:Inositol; D019753:Intramolecular Lyases; C105497:D-myo-inositol-3-phosphate synthase