Papillomavirus particles assembled in 293TT cells are infectious in vivo.
Papillomaviruses (PVs) demonstrate both tissue and species tropisms. Because PVs replicate only in terminally differentiating epithelium, the recent production of infectious PV particles in 293 cells marks an important breakthrough. In this article, we demonstrate that infectious PV particles produced in 293TT cells can cause papillomatous growths in the natural host animal. Moreover, we show that species-matched PV genomes can be successfully delivered in vivo by a heterologous, species-mismatched PV capsid. Additionally, our results indicate that the addition of the simian virus 40 origin of replication to the papillomavirus genome increases the production of infectious papillomavirus particles by increasing genome amplification in the transfected 293TT cells.
Culp
Timothy D
TD
Jake Gittlen Cancer Research Foundation, College of Medicine, Pennsylvania State University, Hershey, PA 17033-2390, USA.
Cladel
Nancy M
NM
Balogh
Karla K
KK
Budgeon
Lynn R
LR
Mejia
Andres F
AF
Christensen
Neil D
ND
D000818:Animals; D002460:Cell Line; D012777:Cottontail rabbit papillomavirus / Q000502:physiology*; D004195:Disease Models, Animal; D006801:Humans; D010212:Papilloma / Q000473:pathology; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology*; D011817:Rabbits; D011995:Recombination, Genetic; D018741:Replication Origin / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics; D014779:Virus Replication / Q000235:genetics