Development and characterization of a murine monoclonal antibody specific for the P1.15 PorA proteins from vaccine strain B:4,7:P1.19,15 of Neisseria meningitidis.
Neisseria meningitidis isolates are conventionally classified by serosubtyping, which characterizes the reactivities of the PorA outer membrane protein variable-region epitopes with monoclonal antibodies (MAbs). New murine hybridomas, secreting specific MAbs against PorA of N. meningitidis serogroup B, were generated using conventional hybridoma procedures. Using outer membrane protein as antigen, we obtained two positive clones, and one of them we characterized. This MAb reacted, on whole-cell enzyme-linked immunosorbent assay (ELISA) and immunoblotting, only with strain subtype P1.15 and its IgG2b isotype. This MAb demonstrated bactericidal activity against the homologous strain in the presence of human complement.
Pérez
M E
ME
Centro de Investigación y Producción de Vacunas y Sueros, Instituto Finlay, La Habana, Cuba. meperez@finlay.edu.cu
Barberá
R
R
Domínguez
F
F
Otero
O
O
Gutiérrez
M
M
Falero-Diaz
G
G
Sotolongo
F
F
Sierra
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000276:immunology*; D005260:Female; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D009345:Neisseria meningitidis / Q000276:immunology*; D018272:Porins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001428:Bacterial Vaccines; D018272:Porins; C057699:porin protein, Neisseria