eIF5A binds to translational machinery components and affects translation in yeast.
The putative translation factor eIF5A is essential for cell viability and is highly conserved from archebacteria to mammals. Although this protein was originally identified as a translation initiation factor, subsequent experiments did not support a role for eIF5A in general translation. In this work, we demonstrate that eIF-5A interacts with structural components of the 80S ribosome, as well as with the translation elongation factor 2 (eEF2). Moreover, eIF5A is further shown to cofractionate with monosomes in a translation-dependent manner. Finally, eIF5A mutants show altered polysome profiles and are sensitive to translation inhibitors. Our results re-establish a function for eIF5A in translation and suggest a role for this factor in translation elongation instead of translation initiation.
Zanelli
Cleslei F
CF
Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, SP 14801-902, Brazil.
Maragno
Ana L C
AL
Gregio
Ana P B
AP
Komili
Suzanne
S
Pandolfi
José R
JR
Mestriner
Carlos A
CA
Lustri
Wilton R
WR
Valentini
Sandro R
SR
D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D009154:Mutation; D010441:Peptide Chain Elongation, Translational; D010448:Peptide Initiation Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000097574:Eukaryotic Translation Initiation Factor 5A
D008239:Lysine; D010448:Peptide Initiation Factors; D011500:Protein Synthesis Inhibitors; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A; C100028:hypusine