title: Obtainment of chelating agents through the enzymatic oxidation of lignins by phenol oxidase.
abstract: Oxidation of lignin obtained from acetosolv and ethanol/water pulping of sugarcane bagasse was performed by phenol oxidases: tyrosinase (TYR) and laccase (LAC), to increase the number of carbonyl and hydroxyl groups in lignin, and to improve its chelating capacity. The chelating properties of the original and oxidized lignins were compared by monitoring the amount of Cu2+ bound to lignin by gel permeation chromatography. The Acetosolv lignin oxidized with TYR was 16.8% and with LAC 21% higher than that of the original lignin. For ethanol/water lignin oxidized with TYR was 17.2% and with LAC 18% higher than that of the original lignin.
authors.affiliation: Biotechnology Department DEBIQ-FAENQUIL, CP 116, CEP 12600-970, Lorena-SP, Brazil.
authors.forename: Gabriela M M
authors.initials: GM
authors.lastname: Calabria
authors.forename: Adilson R
authors.initials: AR
authors.lastname: Gonçalves
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D002482:Cellulose / Q000737:chemistry*; D002614:Chelating Agents / Q000138:chemical synthesis*; D042845:Laccase / Q000737:chemistry*; D008031:Lignin / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D010084:Oxidation-Reduction; D011198:Solanum tuberosum / Q000201:enzymology*
chemical_list: D002614:Chelating Agents; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D042845:Laccase; D014442:Monophenol Monooxygenase