[Studies on the mechanism of Cr-induced DNA damage in glouse].
OBJECTIVE
To investigate the mechanism of Cr-induced DNA damage in glouse.


METHODS
The studies were conducted at three pH values(pH 6.5, 7.5, 8.5) with a range of sugars, including D-glucose, alpha-methyl-D-glucose, 2-deoxy-D-glucose, and D-glucose-C-d7. DNA damage was analyzed by gel electrophoresis, and the new species and their lifetime were detected by EPR.


RESULTS
The results of these experiments have reinforced the postulate that Cr (V) complexes are likely to be involved in the genotoxic damage that eventually results in Cr-induced cancers. With an increase in the pH values, the amount of Form II DNA decreased due to the increasing rate of Cr (V) disproportionation and H concentration. On the other hand, the presence of sugars in the reaction system generated Cr-mixed-ligand complexes, which could also cause DNA cleavage.


CONCLUSIONS
Proposed mechanism of DNA cleavage are presented induced by Cr (V)-mixed-ligand.
Shi
Li-Wei
LW
Institute of Environmental Health and Related Product Safety, China CDC, Beijing 100021, China.
Jing
Hai-Ning
HN
Ren
Gai-Ying
GY
Aviva
Levina
L
D002857:Chromium / Q000633:toxicity*; D053837:DNA Cleavage / Q000187:drug effects; D004249:DNA Damage; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005947:Glucose / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects*
D004269:DNA, Bacterial; D002857:Chromium; D005947:Glucose