Two-dimensional impedance imaging of cell migration and epithelial stratification.
We present a miniaturized impedance imaging system, developed for 2D imaging of cell and tissue culture. The system is based on 16 microelectrodes (5 microm x 4 mm). An equivalent circuit for four-point (tetrapolar) impedance spectra was developed and validated. The system uses an Agilent 4294A impedance analyser combined with a front-end amplifier for the impedance measurements. Human epithelial stem cells (YF 29) were grown on the device surface. Cell migration speeds of 300 nm min(-1) following a "scratch" wound closure assay could be established. Using a commercial software developed for geophysical prospecting, we could generate impedance tomography images at 10 kHz revealing cell migration, increase of epithelial thickness and changes in tissue resistivity over a time course of several days.
Linderholm
Pontus
P
Laboratory of Microsystems, Swiss Federal Institute of Technology, 1015, Lausanne, Switzerland. pontus.linderholm@epfl.ch
Braschler
Thomas
T
Vannod
Jeanne
J
Barrandon
Yann
Y
Brouard
Michel
M
Renaud
Philippe
P
D018929:Cell Culture Techniques; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D017097:Electric Impedance; D004867:Equipment Design; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007231:Infant, Newborn; D015603:Keratinocytes / Q000502:physiology*; D008297:Male; D008839:Microelectrodes; D008904:Miniaturization; D012867:Skin / Q000166:cytology; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*