Inhibition of hepatitis B virus X gene expression by 10-23 DNAzymes.
The X protein (HBx) of human hepatitis B virus (HBV) is a transcriptional activator protein. The HBx protein plays an important role in viral replication in HBV infected cells and the liver diseases including hepatitis, cirrhosis and hepatocellular carcinoma (HCC). Therefore, the repression of HBx gene expression by 10-23 DNAzymes might be a good way to inhibit HBV replication and counteract HBV-related liver diseases. We designed three 10-23 DNAzymes with different substrate-recognition domains. When each of the 10-23 DNAzymes were cotransfected into human AD293 cells with HBx-EGFP expression plasmid, they could all reduce the level of HBx mRNA as well as the HBx-EGFP protein. These results suggest that the 10-23 DNAzymes might be used for gene therapy of liver diseases caused by HBV.
Hou
Wei
W
Key Laboratory of Infectious Diseases, Ministry of Public Health of China, Institute of Infectious Diseases, First Affiliated Hospital, College of Medical Sciences, Zhejiang University, Qingchun Road 79, Hangzhou, Zhejiang 310003, China.
Ni
Qin
Q
Wo
Jianer
J
Li
Minwei
M
Liu
Kezhou
K
Chen
Liwei
L
Hu
Zhongrong
Z
Liu
Ronghua
R
Hu
Minjun
M
D000998:Antiviral Agents / Q000494:pharmacology*; D019677:Artificial Gene Fusion; D002460:Cell Line; D021881:DNA, Catalytic / Q000235:genetics / Q000494:pharmacology*; D004277:DNA, Single-Stranded / Q000235:genetics / Q000494:pharmacology*; D005453:Fluorescence; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins
D000998:Antiviral Agents; D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D012333:RNA, Messenger; D012367:RNA, Viral; C402582:RNA-cleaving DNA 10-23; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D049452:Green Fluorescent Proteins