title: Enhanced production of recombinant nattokinase in Bacillus subtilis by the elimination of limiting factors.
abstract: By systematic investigation, glutamate and a mixture of metal ions were identified as factors limiting the production of nattokinase in Bacillus subtilis. Consequently, in medium supplemented with these materials, the recombinant strain secreted 4 times more nattokinase (260 mg l(-1)) than when grown in the unsupplemented medium.
authors.affiliation: Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, Taichung, Taiwan, Republic of China.
authors.forename: Po Ting
authors.initials: PT
authors.lastname: Chen
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Chao
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D005822:Genetic Vectors; D008954:Models, Biological; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D013381:Subtilisins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D011994:Recombinant Proteins; D013381:Subtilisins; C053771:nattokinase