title: Simultaneous determination of methamphetamine and its metabolite, amphetamine, in urine using a high performance liquid chromatography column-switching method.
abstract: We describe here a simple, precise, and highly sensitive method for the simultaneous determination of methamphetamine (MA) and amphetamine (AM) in urine using a high performance liquid chromatography (HPLC) column-switching method. A PK-2A (Shodex) column was used for extraction and deproteinization, and a CAPCELL PAK SCX semi-micro, polymer-coated cation-exchange column was employed for separation. The urine sample was mixed with an equal volume of borate buffer (0.1M, pH 9.4), and then 100 microl of the mixture was injected into the HPLC column. The column was switched for 6 min, and then 10 min later detection was performed at 210 nm. Recovery yields of the MA and AM spiked in the urine were 93.0-100.4% with a coefficient of variation of less than 1%. The calibration curves of MA and AM were in the range of 0.1-10 microg/ml with good linearity (r(2)=0.999), with the limit of qualification being 0.005 microg/ml. This method of using HPLC with column-switching can be used for both qualification and quantification of MA and its metabolite, AM, in urine, especially in forensic cases.
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki, Kita, Kagawa 761-0793, Japan. kumihash@mxi.netwave.or.jp
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Kumihashi
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ameno
authors.forename: Takayuki
authors.initials: T
authors.lastname: Shibayama
authors.forename: Keisuke
authors.initials: K
authors.lastname: Suga
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyauchi
authors.forename: Mostofa
authors.initials: M
authors.lastname: Jamal
authors.forename: Weihuan
authors.initials: W
authors.lastname: Wang
authors.forename: Ikuo
authors.initials: I
authors.lastname: Uekita
authors.forename: Iwao
authors.initials: I
authors.lastname: Ijiri
mesh_terms: D000661:Amphetamine / Q000652:urine*; D019969:Amphetamine-Related Disorders / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008694:Methamphetamine / Q000652:urine*; D013056:Spectrophotometry, Ultraviolet; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D008694:Methamphetamine; D000661:Amphetamine