Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis.
IS16 is a thiol-sensitive, Q-deficient mutant strain of Escherichia coli. Here, we show that IS16 harbors a mutation in the ubiG gene encoding a methyltransferase required for two O-methylation steps of Q biosynthesis. Complementation of IS16 with either ubiG or ubiX(K-12) reverses this phenotype, suggesting that UbiX may interact with UbiG.
Gulmezian
Melissa
M
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095-1569, USA.
Zhang
Haitao
H
Javor
George T
GT
Clarke
Catherine F
CF
D002262:Carboxy-Lyases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005816:Genetic Complementation Test; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D016415:Sequence Alignment; D014451:Ubiquinone / Q000096:biosynthesis*
D029968:Escherichia coli Proteins; D014451:Ubiquinone; D008780:Methyltransferases; C019616:UbiG protein, E coli; D002262:Carboxy-Lyases; C113304:ubiX protein, E coli