HPV genotyping and HLA II analysis in a pedigree study of pediatric RRP: preliminary results.
OBJECTIVE
This preliminary pedigree study aims at the evaluation of HPV infection and HLA class II alleles as predictive markers in pediatric RRP.


METHODS
We investigated for HPV genotyping and HLA class II polymorphisms all the components of family nucleus where we detected a child born to an HPV infected mother and suffering from RRP.


RESULTS
HPV 11 was detected both in the laryngeal biopsies of two of the three affected babies and in the cervical smear of their mothers. The third child was positive for HPV 6 while his mother harboured a double HPV 6-16 infection. In one family, the HLA-DQB1*0501 allele exerted its protective role. The HLA-DQB1*0301 allele, commonly associated to a high grade of cervical neoplasia and HPV infection, was present in homozygous in one mother and her child. The same allele was found, though in a heterozygous form, in the third patient too.


CONCLUSIONS
Our report is the first attempt to use the pedigree study for the evaluation of HLA class II alleles and HPV infection related to pediatric RRP. This approach could identify genetic markers that may influence disease predisposition and the severity of HPV infection.
Comar
M
M
Department of Public Medicine Sciences, UCO Hygiene and Preventive Medicine, University of Trieste and IRCCS Burlo Garofolo, Via dell'Istria 65/1, 34100 Trieste, Italy. comar@burlo.trieste.it
Fabris
A
A
Vatta
S
S
Pelos
G
G
Zocconi
E
E
Campello
C
C
D000483:Alleles; D002648:Child; D002675:Child, Preschool; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D052140:Human papillomavirus 11 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000821:virology; D008297:Male; D010212:Papilloma / Q000235:genetics* / Q000821:virology; D030361:Papillomavirus Infections / Q000235:genetics* / Q000821:virology; D010375:Pedigree
D006680:HLA Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II