title: Digital quantification using amplified single-molecule detection.
abstract: We describe a scheme for biomolecule enumeration by converting nanometer-scale specific molecular recognition events mediated by rolling-circle amplification to fluorescent micrometer-sized DNA molecules amenable to discrete optical detection. Our amplified single-molecule detection (SMD) approach preserves the discrete nature of the molecular population, allowing multiplex detection and highly precise quantification of molecules over a dynamic range of seven orders of magnitude. We apply the method for sensitive detection and quantification of the bacterial pathogen Vibrio cholerae.
authors.affiliation: Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, 751 85 Uppsala, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Jarvius
authors.forename: Jonas
authors.initials: J
authors.lastname: Melin
authors.forename: Jenny
authors.initials: J
authors.lastname: Göransson
authors.forename: Johan
authors.initials: J
authors.lastname: Stenberg
authors.forename: Simon
authors.initials: S
authors.lastname: Fredriksson
authors.forename: Carlos
authors.initials: C
authors.lastname: Gonzalez-Rey
authors.forename: Stefan
authors.initials: S
authors.lastname: Bertilsson
authors.forename: Mats
authors.initials: M
authors.lastname: Nilsson
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004270:DNA, Circular / Q000737:chemistry / Q000235:genetics*; D015336:Molecular Probe Techniques; D036103:Nanotechnology; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D014734:Vibrio cholerae / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004270:DNA, Circular