[Setup a model for study the interfere between chemokine and immunocyte].
OBJECTIVE
To investigated the effect of chemoattractant of rhTNF-alpha on TB-LCL. Set up a model for study the interaction between cytokine and B cells.


METHODS
PTC were transfected with Epstein Barr virus, to get tonsil B lymphoblastic cell line(TB-LCL).


RESULTS
It was found rhTNF-alpha can chemotactic TB-LCL, in a dose-dependent manner and this chemotactic effect can be blocked by the neutralizing antibody E6 against TNF.


CONCLUSIONS
This is an useful model in study the interfere between chemokine and immunocyte.
Min
Mike
M
Department of Otolaryngology, No. 117 Hospital of PLA, Hangzhou 310004, China. meek@263.net
Ma
Chaowu
C
Zhan
Yuqin
Y
Xu
Jianzhong
J
Sun
Hao
H
D001402:B-Lymphocytes / Q000276:immunology* / Q000821:virology; D002461:Cell Line, Transformed; D002633:Chemotaxis; D006801:Humans; D014066:Palatine Tonsil / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha