[Progesterone-modulated proteins in human endometrial cancer cell line Ishikawa].
OBJECTIVE
To identify proteins associated with growth inhibitory effects of progesterone in Ishikawa endometrial adenocarcinoma cell line (Ishikawa).


METHODS
The total cellular proteins of the control and megestrol acetate (MA)-treated Ishikawa cells were separated by two-dimensional gel electrophoresis. After colloidal Coomassie G-250 staining and scanning, the gel images were analyzed by PDQuest software. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and Mascot software were used to identify the differentially expressed protein.


CONCLUSIONS
Well-resolved and reproducible 2-dimensional patterns of both control and MA-treated Ishikawa cells were obtained. Forty-one proteins displayed differential expression after MA treatment, among which 23 were up-regulated and 18 down-regulated. Peptide mass fingerprints (PMF) by MALDI-TOF-MS analysis and search in NCBInr resulted in the identification of 8 proteins. The up-regulated proteins identified were catechol-O-methyltransferase (COMT), glutathione S-transferase, keratin 8, splicing factor 3a (subunit 3), chaperonin containing TCP1 (subunit 6A isoform a, CCT6A), RAB6A protein and AHA1. The down-regulated protein was peptidyl prolyl isomerase A (isoform 1). These findings can be helpful in further investigation of the molecular mechanism of progesterone.
Zhang
Yu
Y
Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha 410078, China. yjm0000@hotmail.com
Zhang
Yi
Y
Lin
Qiu-hua
QH
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D019290:Megestrol Acetate / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D015854:Up-Regulation / Q000187:drug effects
D011506:Proteins; D020543:Proteome; D011374:Progesterone; D019290:Megestrol Acetate