title: Regulation of avilamycin biosynthesis in Streptomyces viridochromogenes: effects of glucose, ammonium ion, and inorganic phosphate.
abstract: Effects of glucose, ammonium ions and phosphate on avilamycin biosynthesis in Streptomyces viridochromogenes AS4.126 were investigated. Twenty grams per liter of glucose, 10 mmol/L ammonium ions, and 10 mmol/L phosphate in the basal medium stimulated avilamycin biosynthesis. When the concentrations of glucose, ammonium ions, and phosphate in the basal medium exceeded 20 g/L, 10 mmol/L, and 10 mmol/L, respectively, avilamycin biosynthesis greatly decreased. When 20 g/L glucose was added at 32 h, avilamycin yield decreased by 70.2%. Avilamycin biosynthesis hardly continued when 2-deoxy-glucose was added into the basal medium at 32 h. There was little influence on avilamycin biosynthesis with the addition of the 3-methyl-glucose (20 g/L) at 32 h. In the presence of excess (NH4)2SO4 (20 mmol/L), the activities of valine dehydrogenase and glucose-6-phosphate dehydrogenase were depressed 47.7 and 58.3%, respectively, of that of the control at 48 h. The activity of succinate dehydrogenase increased 49.5% compared to the control at 48 h. The intracellular adenosine triphosphate level and 6-phosphate glucose content of S. viridochromogenes were 128 and 129%, respectively, of that of the control at 48 h, with the addition of the 40 mmol/L of KH2PO4. As a result, high concentrations of glucose, ammonium ions, and inorganic phosphate all led to the absence of the precursors for avilamycin biosynthesis and affected antibiotic synthesis.
authors.affiliation: Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, 30022, Tianjin, People's Republic of China.
authors.forename: Chuan-he
authors.initials: CH
authors.lastname: Zhu
authors.forename: Fu-ping
authors.initials: FP
authors.lastname: Lu
authors.forename: Ya-nan
authors.initials: YN
authors.lastname: He
authors.forename: Zhen-lin
authors.initials: ZL
authors.lastname: Han
authors.forename: Lian-xiang
authors.initials: LX
authors.lastname: Du
mesh_terms: D019325:3-O-Methylglucose / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D003847:Deoxyglucose / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D009844:Oligosaccharides / Q000096:biosynthesis*; D010710:Phosphates / Q000494:pharmacology*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013302:Streptomyces / Q000378:metabolism*; D050802:Valine Dehydrogenase (NADP+) / Q000037:antagonists & inhibitors
chemical_list: D003470:Culture Media; D004791:Enzyme Inhibitors; D009844:Oligosaccharides; D010710:Phosphates; D000644:Quaternary Ammonium Compounds; D019325:3-O-Methylglucose; C006653:avilamycin; D003847:Deoxyglucose; D050802:Valine Dehydrogenase (NADP+); C085852:glucose-3-phosphatase; D010744:Phosphoric Monoester Hydrolases; D005947:Glucose