Degradation of cellulosome-produced cello-oligosaccharides by an extracellular non-cellulosomal beta-glucan glucohydrolase, BglA, from Clostridium cellulovorans.
Clostridium cellulovorans degrades cellulose efficiently to small oligosaccharides, which are used as an energy source. To characterize enzymes related to degrading small oligosaccharides, a gene was cloned for an extracellular non-cellulosomal beta-glucan glucohydrolase (BglA) classified as a family-1 glycosyl hydrolase in C. cellulovorans. Recombinant BglA (rBglA) had higher activity on long glucooligomers than on cellobiose. When cellulosomes and rBglA were incubated with cellulose, the oligosaccharides produced were degraded more effectively to cellobiose and glucose, than with cellulosomes alone, indicating that BglA facilitated the degradation of accessible cello-oligosaccharides produced from cellulose by C. cellulovorans cellulosomes. Thus, this is an example of an extracellular non-cellulosomal enzyme working in a cooperative manner with cellulosomes to degrade cellulose to sugars.
Kosugi
Akihiko
A
Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki 305-8686, Japan.
Arai
Takamitsu
T
Doi
Roy H
RH
D002241:Carbohydrates / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry*; D044603:Cellulosomes / Q000737:chemistry*; D046974:Clostridium cellulovorans / Q000201:enzymology*; D044643:Glucan 1,4-beta-Glucosidase / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D047071:beta-Glucans / Q000737:chemistry*
D002241:Carbohydrates; D009844:Oligosaccharides; D011994:Recombinant Proteins; D047071:beta-Glucans; D002244:Carbon; D002480:Cellulase; D044643:Glucan 1,4-beta-Glucosidase