[Mechanism of myocardial cell apoptosis during delayed preconditioning induced by Sini decoction].
OBJECTIVE
The present study was designed to determine the mechanism of myocardial cell apoptosis during delayed preconditioning induced by Sini decoction (SND).


METHODS
Sprage-Dawleytrats were divide into four groups: control, sham, I/R and SND groups. Left anterior descending coronary artery (LAD) of the rats in I/R group were occluded for h and reperfused for h. SND group rates were pretreated with Sini decoction (5 g x kg(-1) x d(-1)) for three days and the last treatment was pretreated 24h before the index occlusion. Cell apoptosis was measured by flow cytometry, mRNA level of bcl-xl/bcl-xs was detected by RT-PCR and apoptosis inducing factor (AIF) was detected by western blotting.


RESULTS
SND pretreatment for 24 hours could decrease cell apoptosis, increase bcl-xl-bcl-xs mRNA ratio and decrease release of AIF from mitochondria.


CONCLUSIONS
The delayed preconditioning induced by SND can decrease myocardial cell apoptosis. The mechanism is related to mitochondria protection.
Liu
Ying
Y
Department of Pathophysiology, Guangdong Pharmacological College, Guangzhou 510224, China. lylygz@hotmail.com
Chen
Chen
C
Wu
Wei-Kang
WK
Duan
Xin-Feng
XF
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D051020:bcl-X Protein / Q000096:biosynthesis / Q000235:genetics
D051033:Apoptosis Inducing Factor; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D051020:bcl-X Protein