title: Detection and quantitation of circulating Plasmodium falciparum DNA by polymerase chain reaction.
abstract: This chapter describes the application of polymerase chain reaction (PCR) for the detection and quantitation of Plasmodium falciparum DNA in the plasma of malaria-infected individuals. The procedure includes the following protocols: plasma sample preparation, DNA extraction, detection of P. falciparum DNA in the plasma by nested PCR, and quantitation of P. falciparum DNA in the plasma by real-time PCR technology.
authors.affiliation: Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK.
authors.forename: Shira
authors.initials: S
authors.lastname: Gal
authors.forename: James S
authors.initials: JS
authors.lastname: Wainscoat
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008288:Malaria / Q000097:blood* / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D004247:DNA