Effects of genetic polymorphisms CYP1A1, GSTM1, GSTT1 and GSTP1 on urinary 1-hydroxypyrene levels in sugarcane workers.
Sugarcane workers in Brazil are exposed to various genotoxic compounds, including polycyclic aromatic hydrocarbons (PAHs), derived from an incomplete combustion process of burnt sugarcane fields. The effects of the occupational exposure to sugarcane fields burning were measured in urine samples of sugarcane workers from the northwest of the State of São Paulo when exposed (harvesting) and when non-exposed (non-harvesting). The urinary levels of 1-hydroxypyrene (1-OHP) and the influence of the genetic polymorphisms CYP1A1, GSTM1, GSTT1 and GSTP1 were evaluated. Our results showed that the 1-OHP levels were significantly higher (P<0.0000) in the exposed sugarcane workers (0.318 mumol mol(-1) creatinine) than in the non-exposed workers (0.035 mumol mol(-1) creatinine). In an unvaried analysis, no influence regarding the polymorphisms was observed. However, multivariate regression analysis showed that the CYP1A1()4 polymorphism in the exposed group, and age and the GSTP1 polymorphism in the non-exposed group significantly influenced urinary 1-OHP excretion levels (P<0.10). The same group of sugarcane workers was significantly more exposed to PAHs during the harvesting period than during the non-harvesting period.
do Vale Bosso
Rosa Maria
RM
Biology Department, UNESP, São Paulo State University, São José do Rio Preto, SP, Brazil.
Amorim
Lidia Maria Fonte
LM
Andrade
Sandro José
SJ
Rossini
Ana
A
de Marchi
Mary Rosa Rodrigues
MR
de Leon
Antonio Ponce
AP
Carareto
Claudia Marcia Aparecida
CM
Conforti-Froes
Nívea Dulce Tedeschi
ND
D000328:Adult; D000368:Aged; D000383:Agriculture; D015415:Biomarkers / Q000652:urine; D001938:Brazil; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D004784:Environmental Monitoring; D005260:Female; D051549:Glutathione S-Transferase pi / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D011110:Polymorphism, Genetic; D011721:Pyrenes / Q000378:metabolism*; D031786:Saccharum; D016482:Urinalysis
D015415:Biomarkers; D019363:Cytochrome P-450 CYP1A1; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D011721:Pyrenes; C413545:glutathione S-transferase T1; C033146:1-hydroxypyrene