Evaluation of solid-phase microextraction desorption parameters for fast GC analysis of cocaine in coca leaves.
By its simplicity and rapidity, solid-phase microextraction (SPME) appears as an interesting alternative for sample introduction in fast gas chromatography (fast GC). This combination depends on numerous parameters affecting the desorption step (i.e., the release of compounds from the SPME fiber coating to the GC column). In this study, different liner diameters, injection temperatures, and gas flow rates are evaluated to accelerate the thermal desorption process in the injection port. This process is followed with real-time direct coupling a split/splitless injector to a mass spectrometer by means of a short capillary. It is shown that an effective, quantitative, and rapid transfer of cocaine (COC) and cocaethylene (CE) is performed with a 0.75-mm i.d. liner, at 280 degrees C and 4 mL/min gas flow rate. The 7-microm polydimethylsiloxane (PDMS) coating is selected for combination with fast GC because the 100-microm PDMS fiber presents some limitations caused by fiber bleeding. Finally, the developed SPME-fast GC method is applied to perform in less than 5 min, the quantitation of COC extracted from coca leaves by focused microwave-assisted extraction. An amount of 7.6 +/- 0.5 mg of COC per gram of dry mass is found, which is in good agreement with previously published results.
Ilias
Yara
Y
Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences EPGL, University of Geneva, University of Lausanne, 20, Bd. d'Yvoy, 1211 Geneva 4, Switzerland.
Bieri
Stefan
S
Christen
Philippe
P
Veuthey
Jean-Luc
JL
D002849:Chromatography, Gas / Q000379:methods*; D003041:Coca / Q000737:chemistry*; D003042:Cocaine / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results
D003042:Cocaine