Preparation, characterization and in vitro drug release studies of novel polymeric nanoparticles.
Polymeric nanoparticles of AADG cross-linked with MBA encapsulating water soluble macromolecules such as FITC-Dextran have been prepared in the reverse micellar system. The particles obtained were of >85nm in diameter which were highly monodisperse. An optically clear solution was obtained on redispersing these nanoparticles in aqueous buffer. Size and morphology of the particles remains the same on re-dispersing the lyophilized powder of these nanoparticles in aqueous buffer. The size dependency of the particles on the monomer and surfactant concentration was observed. The average size of the nanoparticles as obtained from DLS studies ranges from 74 to 114nm in case 0.06M AOT and 62-104nm in case of 0.1M AOT concentration. FITC-Dextran was entrapped into nanoparticles with high efficiency (>70%). The pH dependent release of the entrapped molecules from these nanoparticles was also studied. At pH 5.0 solution, approximately 43% of FITC-Dx was released and at pH 7.4 it was about 70%.
Nimesh
Surendra
S
Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110007, India.
Manchanda
Romila
R
Kumar
Rupesh
R
Saxena
Amit
A
Chaudhary
Preeti
P
Yadav
Veena
V
Mozumdar
Subho
S
Chandra
Ramesh
R
D000178:Acrylamides / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry; D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000138:chemical synthesis / Q000737:chemistry; D003911:Dextrans / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D004339:Drug Compounding / Q000379:methods; D016503:Drug Delivery Systems / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000737:chemistry / Q000493:pharmacokinetics; D005944:Glucosamine / Q000031:analogs & derivatives / Q000737:chemistry; D008027:Light; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D012542:Scattering, Radiation; D017550:Spectroscopy, Fourier Transform Infrared; D013386:Succinates / Q000737:chemistry; D000099166:Polyacrylamides
D000178:Acrylamides; D000179:Acrylates; D000180:Acrylic Resins; D003692:Delayed-Action Preparations; D003911:Dextrans; D004337:Drug Carriers; D016650:Fluorescein-5-isothiocyanate; D005944:Glucosamine; D011108:Polymers; D013386:Succinates; D000099166:Polyacrylamides; C512554:1,4-bis(2-ethylhexyl) sodium sulfosuccinate; C015219:fluorescein isothiocyanate dextran; C026200:acryloyl chloride; C021221:N,N'-methylenebisacrylamide