title: Rapid production of Candida albicans chlamydospores in liquid media under various incubation conditions.
abstract: The production of chlamydospores is a diagnostic tool used to identify Candida albicans; these structures also represent a model for morphogenetic research. The time required to produce them with standard methods is 48-72 hours in rice meal agar and tensoactive agents. This time can be shorted using liquid media such as cornmeal broth (CMB) and dairy supplements. Five media were tested: CMB plus 1% Tween-80, CMB plus 5% milk, CMB plus 5% milk serum, milk serum, and milk serum plus 1% Tween-80, under different incubation conditions: at 28 degrees C and 37 degrees C in a metabolic bath stirring at 150 rpm, and at 28 degrees C in a culture stove. The reading time points were established at 8 and 16 hours. The best results were obtained at 16 hours with CMB plus 5% milk under incubation at 28 degrees C and stirring at 150 rpm. The next most efficient methods were CMB plus 5% milk serum and CMB plus 1% Tween-80, under the same incubation conditions. The other media were ineffective in producing chlamydospores. The absence of stirring at 28 degrees C prevented the formation of chlamydospores within the set time points, and incubation at 37 degrees C decreased their production. This paper reports that the time to form C. albicans chlamydospores can be reduced.
authors.affiliation: Mycology Laboratory of Chemistry School, Universidad Nacional Autónoma de San Luis Portosí, México.
authors.forename: Zavalza-Stiker
authors.initials: ZS
authors.lastname: Alicia
authors.forename: Ortiz-Saldivar
authors.initials: OS
authors.lastname: Blanca
authors.forename: García-Hernández
authors.initials: GH
authors.lastname: Mariana
authors.forename: Castillo-Casanova
authors.initials: CC
authors.lastname: Magdalena
authors.forename: Bonifaz
authors.initials: B
authors.lastname: Alexandro
mesh_terms: D002176:Candida albicans / Q000502:physiology*; D003470:Culture Media; D008828:Microbiological Techniques
chemical_list: D003470:Culture Media