title: Encapsulation of single enzyme in nanogel with enhanced biocatalytic activity and stability.
abstract: Single protein encapsulated into nanogels with uniformed size and controllable shell thickness were prepared by surface acryloylation of a protein molecule followed by aqueous in situ polymerization. Compared to its free counterpart, the encapsulated protein exhibits similar biocatalytic behavior and significantly improved stability at high temperature and in the presence of organic solvent.
authors.affiliation: Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.forename: Jun
authors.initials: J
authors.lastname: Ge
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Pingkai
authors.initials: P
authors.lastname: Ouyang
mesh_terms: D002384:Catalysis; D004795:Enzyme Stability; D005782:Gels; D006735:Horseradish Peroxidase / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures
chemical_list: D005782:Gels; D006735:Horseradish Peroxidase