title: Generation of a reporter cell line for detection of infectious varicella-zoster virus and its application to antiviral studies.
abstract: To simplify the titration of infectious varicella-zoster virus (VZV), we generated a reporter cell line that produced luciferase in a dose-dependent manner upon infection with cell-free VZV. A few VZV-infected cells were detectable by coculturing with the cell line. We demonstrated the usefulness of the cell line for antiviral studies.
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.
authors.forename: Guan-Qing
authors.initials: GQ
authors.lastname: Wang
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suzutani
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Fukui
authors.forename: Naoki
authors.initials: N
authors.lastname: Nozawa
authors.forename: D Scott
authors.initials: DS
authors.lastname: Schmid
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Naoki
authors.initials: N
authors.lastname: Inoue
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D002460:Cell Line / Q000201:enzymology* / Q000821:virology*; D017930:Genes, Reporter / Q000235:genetics; D014645:Herpesvirus 3, Human / Q000187:drug effects / Q000235:genetics*; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000379:methods*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic
chemical_list: D000998:Antiviral Agents; D008156:Luciferases