Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis.
A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV-Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA-85B or DNA-(85B-Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung-1 in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette-Guérin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.
Pardini
Manuela
M
Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Rome, Italy.
Giannoni
Federico
F
Palma
Carla
C
Iona
Elisabetta
E
Cafaro
Aurelio
A
Brunori
Lara
L
Rinaldi
Monica
M
Fazio
Vito Michele
VM
Laguardia
Maria Elena
ME
Carbonella
Damiano Cosimo
DC
Magnani
Mauro
M
Ensoli
Barbara
B
Fattorini
Lanfranco
L
Cassone
Antonio
A
D000217:Acyltransferases / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D005260:Female; D015696:Gene Products, tat / Q000235:genetics / Q000276:immunology*; D015756:Genes, tat; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D009163:Mycobacterium bovis / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000276:immunology*; D041681:NIH 3T3 Cells; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology; D014162:Transfection; D014376:Tuberculosis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D032581:Tuberculosis Vaccines / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*; D054322:tat Gene Products, Human Immunodeficiency Virus
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015696:Gene Products, tat; D007074:Immunoglobulin G; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; D054322:tat Gene Products, Human Immunodeficiency Virus; D007371:Interferon-gamma; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis