[Screening of proteins interacting with hepatitis C virus NS3 from T7-phage display library].
OBJECTIVE
To screen and identify proteins that interact with hepatitis C virus NS3 by means of T7-phage display system.


METHODS
Hepatitis C virus NS3 was expressed by prokaryotic expression and used as a selected molecule to biopan the T7 select human liver cDNA library; the selected positive clones were identified using DNA sequencing and analyzed with BLAST program in GenBank.


RESULTS
After BLAST analysis in all the positive clones, the proteins which interacted with the hepatitis C virus NS3 were found to be serpin peptidase inhibitor, clade A, member 1 (SERPINA1) and cyclophilin-LC.


CONCLUSIONS
T7-phage display system is a convenient, rapid and effective method for screening interacting proteins. The proteins thus selected will provide an important means for studying the pathogenesis and carcinogenesis of HCV.
Huang
Ying
Y
Molecular Biology Laboratory of Infectious Diseases, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Cai
Xue-fei
XF
He
Mao-rui
MR
Zhang
Jun
J
Huang
Ai-long
AL
D002460:Cell Line; D015723:Gene Library; D016174:Hepacivirus / Q000378:metabolism*; D006801:Humans; D019151:Peptide Library; D025941:Protein Interaction Mapping / Q000379:methods*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism
D019151:Peptide Library; D014760:Viral Fusion Proteins; D017361:Viral Nonstructural Proteins