title: Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis.
abstract: Monolithic materials were prepared in capillaries by in situ polymerization of acrylamide, glycidyl methacrylate, and N,N'-methylenebisacrylamide in the presence of 1,4-butanediol, dodecanol, and DMSO as porogens. With Ampholine attached to the surface of the porous monolith via epoxide groups, a monolithic-IPG (M-IPG) was formed and showed good mechanical and chemical stability. With such a column immobilized by Ampholine 3.5-10, IEF-MIX 3.6-9.3 was separated and good linearity was obtained. The CIEF behavior of M-IPG was evinced by comparing the current with that in the open tubular capillary. In addition, the protein mixtures excreted from lung cancer cells of rats were analyzed with such a new M-IPG column.
authors.affiliation: National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
authors.forename: Guijie
authors.initials: G
authors.lastname: Zhu
authors.forename: Huiming
authors.initials: H
authors.lastname: Yuan
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liang
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D002072:Butylene Glycols / Q000737:chemistry*; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D007851:Dodecanol / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016062:Porosity; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D002072:Butylene Glycols; D004121:Dimethyl Sulfoxide; D007851:Dodecanol; D011506:Proteins; D000099166:Polyacrylamides; C039681:1,4-butanediol