The use of Fourier transform infrared spectroscopy to differentiate Escherichia coli O157:H7 from other bacteria inoculated into apple juice.
Fourier transform infrared spectroscopy (FT-IR) can discriminate Escherichia coli O157:H7 ATCC 35150 from other bacteria: E. coli ATCC 25522, Bacillus cereus ATCC 10876, and Listeria innocua ATCC 51742 inoculated in to apple juice. Spectra of bacterial suspensions (ca. 10(9) cfu/ml in 0.9% NaCl) on Anodisc (aluminum oxide) filters were tested. Unique FT-IR vibrational combination bands from mid-IR active components of bacterial cells are present in the "fingerprint region" at wavenumbers between 1500 and 800 cm(-1). Principal component analysis (PCA) revealed clear segregations between different bacterial strains. Also, soft independent modeling of class analogy (SIMCA) correctly classified E. coli O157:H7 ATCC 35150 from E. coli ATCC 25522 at an 82% confidence level; whereas a 77% confidence level was obtained when using SIMCA to classify E. coli O157:H7 from three other bacterial strains.
Al-Holy
Murad A
MA
Department of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite University, Zarqa-Jordan.
Lin
Mengshi
M
Cavinato
Anna G
AG
Rasco
Barbara A
BA
D001628:Beverages / Q000032:analysis / Q000382:microbiology; D019453:Escherichia coli O157 / Q000145:classification / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D027845:Malus / Q000382:microbiology*; D025341:Principal Component Analysis; D012680:Sensitivity and Specificity; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*