title: Biofilm fermentation of iturin A by a recombinant strain of Bacillus subtilis 168.
abstract: Bacillus subtilis 168 produces thin and fragile biofilm in the static culture, however, it was found out that its transformant B. subtilis RM/iSd16 containing wild sfp, itu operon and degQ, which produced lipopeptide antibiotic iturin A, produced thick and much stable biofilm. Production of iturin A by RM/iSd16 in biofilm was almost two times higher compared to that in the submerged culture at 28 degrees C.
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan. rahman@res.titech.ac.jp
authors.forename: Mohammad Shahedur
authors.initials: MS
authors.lastname: Rahman
authors.forename: Takashi
authors.initials: T
authors.lastname: Ano
authors.forename: Makoto
authors.initials: M
authors.lastname: Shoda
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development*; D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms / Q000254:growth & development*; D009876:Operon / Q000235:genetics; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000254:growth & development; D010456:Peptides, Cyclic / Q000096:biosynthesis* / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C091639:DegQ protein, Bacillus sp.; D010456:Peptides, Cyclic; D015534:Trans-Activators; C013579:iturin A