title: Molecular analysis of the Imatinib-induced complete cytogenetic response in chronic myelogenous leukemia.
abstract: The aim of this study was to elucidate the relationship between clonal and normal haematopoiesis in chronic myelogenous leukemia (CML). Thirteen female patients who reached complete cytogenetic response (CCR) after Imatinib treatment were studied. Although all the study patients exhibited a polyclonal pattern of X inactivation, p210 BCR/ABL transcript remained detectable in all cases by nested RT-PCR. This study also demonstrated the presence of p190 transcript in nine out of 13 study patients. A longer follow-up analysis is needed to clarify the prognostic value of these results. The recent observation that clonal cytogenetic abnormalities may occur in CML patients in polyclonal remission after imatinib suggests that a neoplastic stem cell lacking BCR/ABL rearrangement may acquire further cytogenetic alterations other than Philadelphia chromosome.
authors.affiliation: Department of Haematology and Oncology, Azienda Ospedale S.Martino e Cliniche Universitarie Convenzionate, Genova, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Miglino
authors.forename: Raffaella
authors.initials: R
authors.lastname: Grasso
authors.forename: Riccardo
authors.initials: R
authors.lastname: Varaldo
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Fugazza
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Colombo
authors.forename: Marino
authors.initials: M
authors.lastname: Clavio
authors.forename: Letizia
authors.initials: L
authors.lastname: Canepa
authors.forename: Anna
authors.initials: A
authors.lastname: Garuti
authors.forename: Adalberto
authors.initials: A
authors.lastname: Ibatici
authors.forename: Ivana
authors.initials: I
authors.lastname: Pierri
authors.forename: Filippo
authors.initials: F
authors.lastname: Ballerini
authors.forename: Mario
authors.initials: M
authors.lastname: Sessarego
authors.forename: Marco
authors.initials: M
authors.lastname: Gobbi
mesh_terms: D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001549:Benzamides; D003720:Densitometry; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D000068877:Imatinib Mesylate; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy*; D008297:Male; D008875:Middle Aged; D010879:Piperazines / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012737:Sex Factors; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D001549:Benzamides; D010879:Piperazines; D011743:Pyrimidines; D000068877:Imatinib Mesylate; D016044:Fusion Proteins, bcr-abl