Sexually dimorphic expression of the genes encoding ribosomal proteins L17 and L37 in the song control nuclei of juvenile zebra finches.
Studies evaluating the role of steroid hormones in sexual differentiation of the zebra finch song system have produced complicated and at times paradoxical results, and indicate that additional factors may be critical. Therefore, in a previous study we initiated a screen for differential gene expression in the telencephalon of developing male and female zebra finches. The use of cDNA microarrays and real-time quantitative PCR revealed increased expression of the genes encoding ribosomal proteins L17 and L37 (RPL17 and RPL37) in the male forebrain as a whole. Preliminary in situ hybridization data then indicated enhanced expression of both these genes in song control regions. Two experiments in the present study quantified the mRNA expression. The first utilized 25-day-old male and female zebra finches. The second compared a separate set of juveniles to adults of both sexes to both re-confirm enhanced expression in juvenile males and to determine whether it is limited to developing animals. In Experiment 1, males exhibited increased expression of both RPL17 and RPL37 compared to females in Area X, the robust nucleus of the arcopallium (RA), and the ventral ventricular zone (VVZ), which may provide neurons to Area X. Experiment 2 replicated the sexually dimorphic expression of these genes at post-hatching day 25, and documented that the sex differences are eliminated or greatly reduced in adults. The results are consistent with the idea that these ribosomal proteins may influence sexual differentiation of Area X and RA, potentially regulating the genesis and/or survival of neurons.
Tang
Yu Ping
YP
Michigan State University, Department of Psychology, 108 Giltner Hall, East Lansing, MI 48824-1101, USA.
Wade
Juli
J
D000375:Aging / Q000502:physiology; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D005260:Female; D046369:Finches / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D008297:Male; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics*; D012727:Sex Characteristics; D012733:Sex Differentiation / Q000235:genetics*; D012726:Sexual Behavior, Animal / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013687:Telencephalon / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D014828:Vocalization, Animal / Q000502:physiology
D018076:DNA, Complementary; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C032493:ribosomal protein L17; C076411:Rpl37a protein, Gallus gallus