Genital CD8+ T cell response to HIV-1 gag in mice immunized by mucosal routes with a recombinant simian adenovirus.
AdC6gag37, an E1-deleted adenovirus recombinant derived from the chimpanzee adenovirus serotype 6 expressing a codon-optimized truncated form of gag of HIV-1, was tested for induction of transgene-specific CD8+ T cell responses upon intranasal or intravaginal immunization of mice. Administration of AdC6gag37 induced gag-specific CD8+ T cells at systemic and mucosal sites. Frequencies of gag-specific CD8+ T cells elicited in the genital tract by intravaginal or intranasal immunizations were substantially increased by intranasal priming followed by intravaginal boosting with the same vector. Additionally, intravaginal immunization with AdC6gag37 increased the amount of gammadelta T cells that could be detected in genital tract.
de Souza
Ana Paula D
AP
Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil.
Haut
Larissa H
LH
Silva
Renata
R
Ferreira
Silvia I A C P
SI
Zanetti
Carlos R
CR
Ertl
Hildegund C J
HC
Pinto
Aguinaldo R
AR
D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D000261:Adenoviruses, Simian / Q000235:genetics* / Q000276:immunology; D000282:Administration, Intravaginal; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D015683:Gene Products, gag / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D005836:Genitalia, Female / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control; D015497:HIV-1 / Q000276:immunology; D018928:Immunity, Mucosal; D051379:Mice; D008807:Mice, Inbred BALB C; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D011995:Recombination, Genetic
D016915:AIDS Vaccines; D015683:Gene Products, gag; D016692:Receptors, Antigen, T-Cell, gamma-delta