Photodynamic therapy suppresses the migration and invasion of head and neck cancer cells in vitro.
Head and neck cancer is highly invasive. It has a tendency to metastasise to regional or distant sites after incomplete treatment. Photodynamic therapy (PDT) is effective in the treatment of head and neck cancers. To investigate the effect of sublethal PDT on the invasiveness of head and neck cancer cells and to elucidate the possible mechanisms, we initiated this study. Two head and neck cancer cell lines, KJ-1 and Ca9-22, were used in this study. Wound healing assay, migration assay, and matrigel invasion assay were used to evaluate the cell migration and invasion. Immunoblotting was performed to investigate the possibly involved signaling pathways. Sublethal PDT significantly suppressed the migration and invasion of both KJ-1 and Ca9-22 cells. Phosphorylation of the focal adhesion kinase (FAK) and its down-stream Src kinase and extracellular signal-regulated kinase (ERK) were also inhibited after sublethal PDT. Sublethal PDT suppresses the migration and invasion of Ca9-22 and KJ-1 cells. Inhibited phosphorylation of the FAK-Src kinase-ERK signaling pathway may be involved in the PDT-induced migration/invasion suppression.
Yang
Ting-Hua
TH
Department of Otolaryngology, National Taiwan University Hospital, National Taiwan University, Chung-Shan South Road, Taipei, Taiwan, ROC.
Chen
Chin-Tin
CT
Wang
Cheng-Ping
CP
Lou
Pei-Jen
PJ
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003094:Collagen; D004338:Drug Combinations; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D007797:Laminin; D008027:Light; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010766:Phosphorylation; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011509:Proteoglycans; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism; D019061:src-Family Kinases / Q000378:metabolism
D004338:Drug Combinations; D007797:Laminin; D017319:Photosensitizing Agents; D011509:Proteoglycans; C059485:matrigel; D014443:Tyrosine; D000622:Aminolevulinic Acid; D003094:Collagen; D051416:Focal Adhesion Protein-Tyrosine Kinases; D019061:src-Family Kinases; D048049:Extracellular Signal-Regulated MAP Kinases