An efficient and versatile approach for the construction of oligonucleotide microarrays.
A new immobilization chemistry for covalent attachment of phosphorylated oligonucleotides on epoxy-activated glass surface via opening of oxirane ring is described. The proposed strategy results in excellent immobilization efficiency, spot homogeneity, and morphology. The constructed microarray was successfully demonstrated for discrimination of nucleotide mismatches.
Mahajan
S
S
Nucleic Acids Research Laboratory, Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, Delhi-110,007, India.
Kumar
P
P
Gupta
K C
KC
D020137:Base Pair Mismatch; D004852:Epoxy Compounds; D005027:Ethylene Oxide; D005898:Glass; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D009841:Oligonucleotides
D004852:Epoxy Compounds; D009841:Oligonucleotides; D005027:Ethylene Oxide