title: Search for basonuclin target genes.
abstract: Basonuclin (Bnc 1) is a transcription factor that has an unusual ability to interact with promoters of both RNA polymerases I and II. The action of basonuclin is mediated through three pairs of evolutionarily conserved zinc fingers, which produce three DNase I footprints on the promoters of rDNA and the basonuclin gene. Using these DNase footprints, we built a computational model for the basonuclin DNA-binding module, which was used to identify in silico potential RNA polymerase II target genes in the human and mouse promoter databases. The target genes of basonuclin show that it regulates the expression of proteins involved in chromatin structure, transcription/DNA-binding, ion-channels, adhesion/cell-cell junction, signal transduction, and intracellular transport. Our results suggest that basonuclin, like MYC, may coordinate transcriptional activities among the three RNA polymerases. But basonuclin regulates a distinctive set of pathways, which differ from that regulated by MYC.
authors.affiliation: Center for Bioinformatics, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Junwen
authors.initials: J
authors.lastname: Wang
authors.forename: Shengliang
authors.initials: S
authors.lastname: Zhang
authors.forename: Richard M
authors.initials: RM
authors.lastname: Schultz
authors.forename: Hung
authors.initials: H
authors.lastname: Tseng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D047369:Chromatin Immunoprecipitation; D019295:Computational Biology; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D014157:Transcription Factors / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: C511595:Bnc1 protein, mouse; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D014157:Transcription Factors; C077914:BNC1 protein, human