title: Storage of spatial information by the maintenance mechanism of LTP.
abstract: Analogous to learning and memory storage, long-term potentiation (LTP) is divided into induction and maintenance phases. Testing the hypothesis that the mechanism of LTP maintenance stores information requires reversing this mechanism in vivo and finding out whether long-term stored information is lost. This was not previously possible. Recently however, persistent phosphorylation by the atypical protein kinase C isoform, protein kinase Mzeta (PKMz), has been found to maintain late LTP in hippocampal slices. Here we show that a cell-permeable PKMz inhibitor, injected in the rat hippocampus, both reverses LTP maintenance in vivo and produces persistent loss of 1-day-old spatial information. Thus, the mechanism maintaining LTP sustains spatial memory.
authors.affiliation: Departments of Physiology, Pharmacology, and Neurology, Robert F. Furchgott Center for Neural and Behavioral Science, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY 11203, USA.
authors.forename: Eva
authors.initials: E
authors.lastname: Pastalkova
authors.forename: Peter
authors.initials: P
authors.lastname: Serrano
authors.forename: Deana
authors.initials: D
authors.lastname: Pinkhasova
authors.forename: Emma
authors.initials: E
authors.lastname: Wallace
authors.forename: André Antonio
authors.initials: AA
authors.lastname: Fenton
authors.forename: Todd Charlton
authors.initials: TC
authors.lastname: Sacktor
mesh_terms: D000818:Animals; D001362:Avoidance Learning; D003213:Conditioning, Psychological; D018891:Dentate Gyrus / Q000187:drug effects / Q000502:physiology; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000008:administration & dosage / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008568:Memory / Q000187:drug effects / Q000502:physiology*; D019580:Perforant Pathway; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D000071456:Sequestosome-1 Protein; D019311:Staurosporine / Q000494:pharmacology; D013997:Time Factors
chemical_list: D006360:Heat-Shock Proteins; D000071456:Sequestosome-1 Protein; C491079:Sqstm1 protein, rat; D011493:Protein Kinase C; C504730:protein kinase M zeta, rat; D019311:Staurosporine