title: Selection and characterisation of a xylitol-derepressed Aspergillus niger mutant that is apparently impaired in xylitol transport.
abstract: Aspergillus niger is known for its biotechnological applications, such as the use of xylanase enzyme for the degradation of hemicellulose. Depending on culture conditions, several polyols may also be accumulated, such as xylitol during D: -xylose oxidation. Also during industrial fermentation of xylose for the production of fuel ethanol by recombinant yeast, xylitol is a by-product. We studied xylitol metabolism by isolating mutants that have impaired xylitol-mediated repression. Genetic and biochemical characterisation revealed that one of these mutants was affected not only in xylitol-mediated carbon repression, but also had impaired xylitol transport.
authors.affiliation: Section Molecular Genetics of Industrial Microorganisms, Wageningen University, Dreijenlaan 2, 6703 HA Wageningen, The Netherlands.
authors.forename: Peter J I
authors.initials: PJ
authors.lastname: van de Vondervoort
authors.forename: Marco J L
authors.initials: MJ
authors.lastname: de Groot
authors.forename: George J G
authors.initials: GJ
authors.lastname: Ruijter
authors.forename: Jaap
authors.initials: J
authors.lastname: Visser
mesh_terms: D000409:Alanine / Q000378:metabolism; D001234:Aspergillus niger / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D001692:Biological Transport; D015966:Gene Expression Regulation, Fungal; D005942:Gluconates / Q000378:metabolism; D009154:Mutation; D014993:Xylitol / Q000378:metabolism*
chemical_list: D005942:Gluconates; D000409:Alanine; C030691:gluconic acid; D014993:Xylitol