[The extraction and separation of an active and available component from Taxus mairei].
To study on the 10-deacetyl baccatin III extract from the leaves and branches of Taxus mairei. A new method was proposed to extract and purify 10-DAB III, which was adopted as the parent nucleus of the high efficacious antitumour drug-taxol and docetaxel in the compositive synthesis. Optimization selection was also made in the extraction and purification conditions of taxol. The purity quotient of 10-DAB III obtained via this technics could reach 91%, with the yield above 70%. It has been testified that the technics was feasible in industrial practice.
Wu
Mian-Bin
MB
Bioengineering Institute, College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China. wumb@zju.edu.cn
Ma
Shu-Zhen
SZ
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000432:Methanol; D024301:Plant Bark / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D043823:Taxoids / Q000032:analysis / Q000302:isolation & purification*; D020946:Taxus / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D043823:Taxoids; C042213:10-deacetylbaccatine III; D000432:Methanol