title: Sensitive inductively coupled plasma mass spectrometry assay for the determination of platinum originating from cisplatin, carboplatin, and oxaliplatin in human plasma ultrafiltrate.
abstract: We present a highly sensitive, rapid method for the determination of platinum originating from the anticancer agents cisplatin, carboplatin, and oxaliplatin in human plasma ultrafiltrate. The method is based on the quantification of platinum by inductively coupled plasma mass spectrometry and allows quantification of 7.50 ng l-1 platinum in only 150 microl of matrix. Sample pretreatment involves dilution of samples with 1% HNO3. Validation fulfilled the most recent FDA guidelines for bioanalytical method validation. Validated ranges of quantification were 7.50 ng l-1 to 1.00x10(5) ng l-1 in plasma ultrafiltrate for all three platinum compounds. The assay is now successfully used to support pharmacokinetic studies in cancer patients treated with cisplatin, carboplatin, or oxaliplatin.
authors.affiliation: Department of Pharmacy & Pharmacology, Slotervaart Hospital/The Netherlands Cancer Institute, Louwesweg 6, 1066 EC Amsterdam, and Department of Biomedical Analysis, Faculty of Pharmaceutical Sciences, Utrecht University, The Netherlands. apebr@slz.nl
authors.forename: E E M
authors.initials: EE
authors.lastname: Brouwers
authors.forename: M M
authors.initials: MM
authors.lastname: Tibben
authors.forename: H
authors.initials: H
authors.lastname: Rosing
authors.forename: M J X
authors.initials: MJ
authors.lastname: Hillebrand
authors.forename: M
authors.initials: M
authors.lastname: Joerger
authors.forename: J H M
authors.initials: JH
authors.lastname: Schellens
authors.forename: J H
authors.initials: JH
authors.lastname: Beijnen
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood / Q000493:pharmacokinetics*; D002138:Calibration; D016190:Carboplatin / Q000097:blood / Q000493:pharmacokinetics*; D002945:Cisplatin / Q000097:blood / Q000493:pharmacokinetics*; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D009944:Organoplatinum Compounds / Q000097:blood / Q000493:pharmacokinetics*; D000077150:Oxaliplatin; D010984:Platinum / Q000097:blood*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D014462:Ultrafiltration
chemical_list: D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D010984:Platinum; D016190:Carboplatin; D002945:Cisplatin