title: Development of automated online gel permeation chromatography-gas chromatograph mass spectrometry for measuring multiresidual pesticides in agricultural products.
abstract: An automated online gel permeation chromatography-gas chromatograph mass spectrometer (GPC-GC/MS) was developed for the rapid determination of residual pesticides in agricultural products. Pesticides were extracted from homogenized food samples with acetonitrile and decontaminated via the matrix solid-phase dispersion (MSPD) technique, using a primary secondary amine as sorbent prior to GPC-GC/MS analysis. A slightly modified preparation method and automated GPC step proved useful in minimizing matrix interference. To evaluate the performance of the system, 97 target pesticides were spiked at a concentration of 0.1mg/kg into a range of food types, including potato, cabbage, carrot, apple, orange, cucumber, and rice. A low flow rate of 0.1 mL/min in GPC resulted in a 40-fold reduction in solvent consumption compared with conventional GPC column applications. The combination of MSPD technique and GPC-GC/MS for the analysis of the 97 pesticides can be accomplished within 90 min. Most pesticides were recovered in the range of 70-120%, with relative standard deviation generally less than 10%. The results demonstrate that the method can be successfully applied with acceptable recoveries to a broad range of target pesticides within a diverse range of food types.
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, PO Box 2871, Beijing 100085, China.
authors.forename: Li-Bin
authors.initials: LB
authors.lastname: Liu
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hashi
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Qin
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Zhou
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Lin
mesh_terms: D001331:Automation; D001937:Brassica / Q000737:chemistry; D002850:Chromatography, Gel / Q000379:methods*; D032084:Citrus sinensis / Q000737:chemistry; D018556:Crops, Agricultural / Q000737:chemistry*; D018553:Cucumis sativus / Q000737:chemistry; D018552:Daucus carota / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D027845:Malus / Q000737:chemistry; D009862:Online Systems; D012275:Oryza / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D015203:Reproducibility of Results; D011198:Solanum tuberosum / Q000737:chemistry
chemical_list: D010573:Pesticide Residues; D010575:Pesticides