The role of grancalcin in adhesion of neutrophils.
Grancalcin is a protein specifically expressed in neutrophils and monocytes/macrophages. The function of grancalcin has not been identified. Grancalcin-deficient neutrophils were previously demonstrated to exert normal recruitment to the inflamed site, NADPH oxidase activation, extracellular release of secondary granules, apoptosis and activation-induced Ca2+ flux. In this study we analyzed granule numbers in resting and activated grancalcin-deficient neutrophils, their phagocytic activity and adherence to extracellular matrix proteins. Results revealed normal phagocytosis and degranulation of grancalcin-deficient neutrophils, while their adhesion to fibronectin was decreased by 60%. Consistently, the processes associated with neutrophil adhesion, such as formation of focal adhesion complexes and spreading, were also impaired in grancalcin-deficient neutrophils by 89 and 38%, respectively. In contrast, adherence to other extracellular matrix proteins: collagen, laminin and vitronectin, was not significantly altered. We thus report for the first time a function of grancalcin.
Xu
Ping
P
Centre for Molecular Medicine, Department of Medicine, The Rayne Institute, University College London, London WC1E 6JJ, UK.
Roes
Jürgen
J
Segal
Anthony W
AW
Radulovic
Marko
M
D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000502:physiology*; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D048430:Cell Shape / Q000235:genetics / Q000502:physiology; D003594:Cytoplasmic Granules / Q000235:genetics / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D022001:Focal Adhesions / Q000235:genetics / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D008854:Microscopy, Electron; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D010587:Phagocytosis / Q000235:genetics / Q000502:physiology*; D013211:Staphylococcus aureus / Q000502:physiology
D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; C503122:Gca protein, mouse; D005340:Fibrinogen