[Anti-HIV-1 effect of compound K3 from flower of Japanese pagoda tree in vitro].
OBJECTIVE
To investigate the anti-HIV properties of compound K3 purified from Japanese pagoda tree flower as well as its anti-HIV mechanism in vitro.


METHODS
The cytotoxicity of compound K3 was detected by MTF assay. The anti-HIV activities was measured by syncytium reduction, p24 antigen production and protection for HIV-1 induced cytopathic effects in various HIV-1 strains acute or chronically infected cells system. The mechanism of action of the compound K3 was determined by inhibiting HIV-1 RT activity, cell-to-cell fusion, virus binding and entry into cells.


CONCLUSIONS
Compound K3 is found to be a potent inhibitor of HIV-1 replication against a various viruses in different cells including laboratory strain (HIV-1 (IIIB)), drug-resistant (HIV-1(74V)) and clinical isolates (HIV-1 (KM018)) of HIV-1. It indicats that K3 act through at least two clarified antiviral mechanisms: inhibition of HIV-1 entry into cell and inhibition of HIV-1 RT activity.
Zhang
Gao-Hon
GH
Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
Zheng
Yong-Tang
YT
D019380:Anti-HIV Agents / Q000494:pharmacology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D047309:Flavones / Q000008:administration & dosage / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D015497:HIV-1 / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D009000:Monocytes / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects; D014197:Trees; D014779:Virus Replication / Q000187:drug effects*
D019380:Anti-HIV Agents; D047309:Flavones; D054303:HIV Reverse Transcriptase