Molecular characterisation of an Australian isolate of Bonamia exitiosa.
An Australian (New South Wales) isolate of Bonamia was characterised at the molecular level by sequencing the 18S-ITS-1 region of the small subunit rRNA operon obtained from flat oysters Ostrea angasi shown to be infected by histological examination. Sequence data alignment with homologous genes from 2 other isolates of Bonamia (New Zealand and France) revealed high levels of nucleotide identity with both isolates. However, the Australian Bonamia is shown to be more closely related to the New Zealand isolate, suggesting the existence of an oceanic subgroup of Bonamia.
Corbeil
Serge
S
AAHL Fish Diseases Laboratory, Australian Animal Health Laboratory, CSIRO Livestock Industries, Private Bag 24, Geelong, Victoria 3220, Australia. serge.corbeil@csiro.au
Arzul
Isabelle
I
Robert
Maeva
M
Berthe
Franck C J
FC
Besnard-Cochennec
Nathalie
N
Crane
Mark St J
MS
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D041602:Haplosporida / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009517:New South Wales; D049895:Ostrea / Q000469:parasitology*; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D016415:Sequence Alignment
D017931:DNA Primers; D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S