title: Enhanced expression of VEGF following bFGF inhibition in non-small cell lung cancer cell lines.
abstract: Inhibition of basic fibroblast growth factor (bFGF) leads to decreased proliferation of non-small cell lung cancer (NSCLC) cell lines. Although it is well known that bFGF stimulates vascular endothelial growth factor (VEGF) secretion, the influence of bFGF-inhibition is unclear. We therefore investigated the influence of bFGF inhibition on VEGF gene expression and secretion in NSCLC cell lines. In our experiments we demonstrated that inhibition of bFGF gene and protein expression induced increased secretion of VEGF. We also observed an increase of VEGF gene expression in these cells. Furthermore, inhibition of bFGF activated the p42/p44 mitogen-activated protein kinase (MAPK). These results demonstrate that inhibition of bFGF appears to stimulate VEGF production in a p42/44 MAPK-dependent manner. These findings suggest that inhibition of bFGF alone is not a promising strategy to inhibit angiogenesis.
authors.affiliation: Department of Respiratory Medicine, University of Leipzig, Germany. kuhnh@medizin.uni-leipzig.de
authors.forename: Hartmut
authors.initials: H
authors.lastname: Kuhn
authors.forename: Jana
authors.initials: J
authors.lastname: Konrad
authors.forename: Silke
authors.initials: S
authors.lastname: Holtz
authors.forename: Aida
authors.initials: A
authors.lastname: Salameh
authors.forename: Christian
authors.initials: C
authors.lastname: Gessner
authors.forename: Stefan
authors.initials: S
authors.lastname: Hammerschmidt
authors.forename: Hubert
authors.initials: H
authors.lastname: Wirtz
mesh_terms: D000911:Antibodies, Monoclonal; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D045744:Cell Line, Tumor; D016222:Fibroblast Growth Factor 2 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism*; D020319:Oligodeoxyribonucleotides, Antisense; D015398:Signal Transduction; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D020319:Oligodeoxyribonucleotides, Antisense; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3