title: Construction of recombinant Bacillus subtilis for production of polyhydroxyalkanoates.
abstract: Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates synthesized by numerous bacteria as intracellular carbon and energy storage compounds and accumulated as granules in the cytoplasm of cells. In this work, we constructed two recombinant plasmids, pBE2C1 and pBE2C1AB, containing one or two PHA synthse genes, respectively. The two plasmids were inserted into Bacillus subtilis DB104 to generate modified strains, B. subtilis/pBE2C1 and B. subtilis/pBE2C1AB. The two recombinants strains were subjected to fermentation and showed PHA accumulation, the first reported example of mcl-PHA production in B. subtilis. Gas Chromatography analysis identified the compound produced by B. subtilis/pBE2C1 to be a hydroxydecanoate-co-hydroxydodecanoate (HD-co-HDD) polymer whereas that produced by B. subtilis/pBE2C1AB was a hydroxybutyrate-co-hydroxydecanoate-co-hydroxydodecanoate (HB-HD-HDD) polymer.
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Wang
authors.forename: Lifang
authors.initials: L
authors.lastname: Ruan
authors.forename: Wai-Hung
authors.initials: WH
authors.lastname: Lo
authors.forename: Hong
authors.initials: H
authors.lastname: Chua
authors.forename: Hoi-Fu
authors.initials: HF
authors.lastname: Yu
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D020118:Cupriavidus necator / Q000201:enzymology* / Q000235:genetics; D024861:Genetic Enhancement / Q000379:methods*; D011108:Polymers / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D011108:Polymers; D011994:Recombinant Proteins; C111948:poly(hydroxyethanoate); D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase