Interstitial and vascular type V collagen morphologic disorganization in usual interstitial pneumonia.
Recent evidence suggests that type V collagen plays a role in organizing collagen fibrils, thus maintaining fibril size and spatial organization uniform. In this study we sought to characterize the importance of type V collagen morphological disorganization and to study the relationship between type V collagen, active remodeling of the pulmonary vascular/parenchyma (fibroblastic foci), and other collagen types in usual interstitial pneumonia (UIP). We examined type V collagen and several other collagens in 24 open lung biopsies with histological pattern of UIP from patients with idiopathic pulmonary fibrosis (IPF). We used immunofluorescence, morphometry, and three-dimensional reconstruction to evaluate the amount of collagen V and its interaction with the active remodeling progression in UIP, as well as types I and III collagen fibers. Active remodeling progression was significantly related to type V collagen density (p<0.05), showing a gradual and direct increase to minimal, moderate, and severe fibrosis degree in UIP and in the three different areas: normal, intervening, and mural-organizing fibrosis in UIP. Parenchymal changes were characterized by morphological disorganization of fibrillar collagen with diverse disarray and thickness when observed by three-dimensional reconstruction. We concluded that in the different temporal stages of UIP, vascular/parenchyma collagen type V is increased, in disarray, and is the most important predictor of survival.
Parra
Edwin Roger
ER
Department of Pathology, University of São Paulo Medical School, São Paulo, Brazil.
Teodoro
Walcy R
WR
Velosa
Ana Paula Pereira
AP
de Oliveira
Cristiane Carla
CC
Yoshinari
Natalino Hajime
NH
Capelozzi
Vera Luiza
VL
D001706:Biopsy; D001808:Blood Vessels / Q000737:chemistry / Q000473:pathology; D024042:Collagen Type I / Q000032:analysis; D024061:Collagen Type III / Q000032:analysis; D024062:Collagen Type V / Q000032:analysis*; D005260:Female; D006801:Humans; D008168:Lung / Q000098:blood supply / Q000737:chemistry / Q000473:pathology*; D017563:Lung Diseases, Interstitial / Q000473:pathology*; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods; D008875:Middle Aged; D011347:Procollagen / Q000032:analysis*; D012680:Sensitivity and Specificity
D024042:Collagen Type I; D024061:Collagen Type III; D024062:Collagen Type V; D011347:Procollagen