Beamline X29: a novel undulator source for X-ray crystallography.
A high-flux insertion device and beamline for macromolecular crystallography has been built at the National Synchrotron Light Source (NSLS) that employs a mini-gap undulator source developed by the NSLS. The mini-gap undulator at beamline X29 is a hybrid-magnet device of period 12.5 mm operating at proven gaps of 3.3-10 mm. The beamline provides hard X-rays for macromolecular crystallography experiments from the second and third harmonics over an energy range of 5-15 keV. The X-ray optics is designed to deliver intense and highly collimated X-rays. Horizontal focusing is achieved by a cryogenically cooled sagittally focusing double-crystal monochromator with approximately 4.1:1 demagnification. A vertical focusing mirror downstream of the monochromator is used for harmonic rejection and vertical focusing. The experimental station hosts an Area Detector Systems Quantum 315 CCD detector with 2.2 s readout time between exposures and Crystal Logic goniostat for crystal rotation and detector positioning. An auto-mounter crystal changer has been installed to facilitate the high-throughput data collection required by the major users, which includes structural genomics projects and the Macromolecular Crystallography Research Resource mail-in program. X29 is 10(3) times brighter than any existing bending-magnet beamline at NSLS with an actual flux of 2.5 x 10(11) photons s(-1) through a 0.12 mm square aperture at 11.271 keV.
Shi
Wuxian
W
Center for Synchrotron Biosciences, Case Proteomics Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Robinson
Howard
H
Sullivan
Michael
M
Abel
Don
D
Toomey
John
J
Berman
Lonny E
LE
Lynch
Don
D
Rosenbaum
Gerd
G
Rakowsky
George
G
Rock
Larry
L
Nolan
Bill
B
Shea-McCarthy
Grace
G
Schneider
Dieter
D
Johnson
Erik
E
Sweet
Robert M
RM
Chance
Mark R
MR
D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D009991:Oscillometry; D017356:Synchrotrons; D013997:Time Factors; D014965:X-Rays