Autocrine role for interleukin-8 in Bartonella henselae-induced angiogenesis.
The gram-negative bacterium Bartonella henselae is capable of causing angiogenic lesions as a result of infection. Previously, it has been shown that B. henselae infection can result in production of the chemokine interleukin-8 (IL-8). In this study, we demonstrated that monocytes, endothelial cells, and hepatocytes produce IL-8 in response to B. henselae infection. We also investigated the role of IL-8 in B. henselae-induced endothelial cell proliferation and capillary tube formation. Both in vitro angiogenesis assays were IL-8 dependent. B. henselae-mediated inhibition of apoptosis, as indicated by gene expression of Bax and Bcl-2, was also shown to be IL-8 dependent in endothelial cells. Furthermore, infection of endothelial cells with B. henselae stimulated upregulation of the IL-8 chemokine receptor CXCR2. Infection of human endothelial cells by B. henselae resulting in IL-8 production likely plays a central role in the ability of this organism to cause angiogenesis during infection.
McCord
Amy M
AM
Department of Molecular Medicine, School of Basic Biomedical Science, College of Medicine, University of South Florida, 12901 Bruce B. Downs Blvd., MDC10, Tampa, FL 33612, USA.
Resto-Ruiz
Sandra I
SI
Anderson
Burt E
BE
D016917:Angiomatosis, Bacillary / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D017209:Apoptosis / Q000235:genetics; D019898:Autocrine Communication; D018416:Bartonella henselae; D002196:Capillaries / Q000254:growth & development; D049109:Cell Proliferation; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D015870:Gene Expression; D022781:Hepatocytes / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D009000:Monocytes / Q000276:immunology; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D023063:Receptors, Interleukin-8B / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein / Q000235:genetics
D007074:Immunoglobulin G; D016209:Interleukin-8; D019253:Proto-Oncogene Proteins c-bcl-2; D023063:Receptors, Interleukin-8B; D051028:bcl-2-Associated X Protein