title: Paramagnetic tagging of diamagnetic proteins for solution NMR.
abstract: In this article, approaches towards the paramagnetic tagging of diamagnetic proteins are reviewed. Alignment can be achieved by adding paramagnetic fusion proteins or peptides to the C- or the N-terminus or by attaching paramagnetic tags to Cystein residues. Applications for the study of homodimer structures and protein/ligand interactions, as well as protein domain dynamics, are reviewed.
authors.affiliation: Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany.
authors.forename: Fernando
authors.initials: F
authors.lastname: Rodriguez-Castañeda
authors.forename: Peter
authors.initials: P
authors.lastname: Haberz
authors.forename: Andrei
authors.initials: A
authors.lastname: Leonov
authors.forename: Christian
authors.initials: C
authors.lastname: Griesinger
mesh_terms: D003545:Cysteine / Q000737:chemistry*; D019281:Dimerization; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
chemical_list: D008024:Ligands; D011506:Proteins; D003545:Cysteine