title: Apoptosis resistance downstream of eIF4E: posttranscriptional activation of an anti-apoptotic transcript carrying a consensus hairpin structure.
abstract: Aberrant activation of the translation initiation machinery is a common property of malignant cells, and is essential for breast carcinoma cells to manifest a malignant phenotype. How does sustained activation of the rate limiting step in protein synthesis so fundamentally alter a cell? In this report, we test the post transcriptional operon theory as a possible mechanism, employing a model system in which apoptosis resistance is conferred on NIH 3T3 cells by ectopic expression of eIF4E. We show (i) there is a set of 255 transcripts that manifest an increase in translational efficiency during eIF4E-mediated escape from apoptosis; (ii) there is a novel prototype 55 nt RNA consensus hairpin structure that is overrepresented in the 5'-untranslated region of translationally activated transcripts; (iii) the identified consensus hairpin structure is sufficient to target a reporter mRNA for translational activation under pro-apoptotic stress, but only when eIF4E is deregulated; and (iv) that osteopontin, one of the translationally activated transcripts harboring the identified consensus hairpin structure functions as one mediator of the apoptosis resistance seen in our model. Our findings offer genome-wide insights into the mechanism of eIF4E-mediated apoptosis resistance and provide a paradigm for the systematic study of posttranscriptional control in normal biology and disease.
authors.affiliation: Department of Medicine, University of Minnesota, MMC 276, Minneapolis, MN 55455, USA.
authors.forename: Ola
authors.initials: O
authors.lastname: Larsson
authors.forename: David M
authors.initials: DM
authors.lastname: Perlman
authors.forename: Danhua
authors.initials: D
authors.lastname: Fan
authors.forename: Cavan S
authors.initials: CS
authors.lastname: Reilly
authors.forename: Mark
authors.initials: M
authors.lastname: Peterson
authors.forename: Cecilia
authors.initials: C
authors.lastname: Dahlgren
authors.forename: Zicai
authors.initials: Z
authors.lastname: Liang
authors.forename: Shunan
authors.initials: S
authors.lastname: Li
authors.forename: Vitaly A
authors.initials: VA
authors.lastname: Polunovsky
authors.forename: Claes
authors.initials: C
authors.lastname: Wahlestedt
authors.forename: Peter B
authors.initials: PB
authors.lastname: Bitterman
mesh_terms: D020121:5' Untranslated Regions / Q000737:chemistry*; D000818:Animals; D017209:Apoptosis; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D023281:Genomics; D051379:Mice; D041681:NIH 3T3 Cells; D009690:Nucleic Acid Conformation; D053495:Osteopontin; D014176:Protein Biosynthesis; D038621:Regulatory Sequences, Ribonucleic Acid; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D039561:Eukaryotic Initiation Factor-4E; D038621:Regulatory Sequences, Ribonucleic Acid; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D053495:Osteopontin