CE-LIF coupled with flow cytometry for high-throughput quantitation of fluorophores in single intact cells.
We report a method of coupled CE-LIF detection with flow cytometry for high-throughput determination and quantitation of fluorophores in single intact K562/S (KS) cells. The membrane properties of KS cell including fluophore transport rate and apparent permeability coefficient were further quantitatively characterized. The method has advantages for accurate quantitation and unique capacity of high-throughput analysis. The strategy will be useful for the quantitation of fluorophores in the intact cells, such as measurement of multidrug resistance, quantitation of specific protein expression, and quantitative characterization of protein and enzyme functions.
Xiao
Hua
H
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, PR China.
Li
Xin
X
Zou
Hanfa
H
Yang
Ling
L
Wang
Yulin
Y
Wang
Hailin
H
Le
X Chris
XC
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis; D002138:Calibration; D002463:Cell Membrane Permeability / Q000502:physiology*; D018432:Drug Resistance, Multiple; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D020014:K562 Cells; D020112:Rhodamine 123 / Q000737:chemistry
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D005456:Fluorescent Dyes; D020112:Rhodamine 123