title: Maximum growth rates and possible life strategies of different bacterioplankton groups in relation to phosphorus availability in a freshwater reservoir.
abstract: We investigated net growth rates of distinct bacterioplankton groups and heterotrophic nanoflagellate (HNF) communities in relation to phosphorus availability by analysing eight in situ manipulation experiments, conducted between 1997 and 2003, in the canyon-shaped Rímov reservoir (Czech Republic). Water samples were size-fractionated and incubated in dialysis bags at the sampling site or transplanted into an area of the reservoir, which differed in phosphorus limitation (range of soluble reactive phosphorus concentrations--SRP, 0.7-96 microg l-1). Using five different rRNA-targeted oligonucleotide probes, net growth rates of the probe-defined bacterial groups and HNF assemblages were estimated and related to SRP using Monod kinetics, yielding growth rate constants specific for each bacterial group. We found highly significant differences among their maximum growth rates while insignificant differences were detected in the saturation constants. However, the latter constants represent only tentative estimates mainly due to insufficient sensitivity of the method used at low in situ SRP concentrations. Interestingly, in these same experiments HNF assemblages grew significantly faster than any bacterial group studied except for a small, but abundant cluster of Betaproteobacteria (targeted by the R-BT065 probe). Potential ecological implications of different growth capabilities for possible life strategies of different bacterial phylogenetic lineages are discussed.
authors.affiliation: Biological Centre of the Academy of Sciences of the Czech Republic, Hydrobiological Institute, Na Sádkách 7, CZ-37005 Ceské Budejovice, Czech Republic. ksimek@hbu.cas.cz
authors.forename: Karel
authors.initials: K
authors.lastname: Simek
authors.forename: Karel
authors.initials: K
authors.lastname: Hornák
authors.forename: Jan
authors.initials: J
authors.lastname: Jezbera
authors.forename: Jirí
authors.initials: J
authors.lastname: Nedoma
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Vrba
authors.forename: Viera
authors.initials: V
authors.lastname: Straskrábová
authors.forename: Miroslav
authors.initials: M
authors.lastname: Macek
authors.forename: John R
authors.initials: JR
authors.lastname: Dolan
authors.forename: Martin W
authors.initials: MW
authors.lastname: Hahn
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000254:growth & development* / Q000378:metabolism; D015169:Colony Count, Microbial / Q000379:methods; D017753:Ecosystem; D056890:Eukaryota / Q000254:growth & development* / Q000378:metabolism; D005618:Fresh Water / Q000382:microbiology*; D017404:In Situ Hybridization, Fluorescence; D007700:Kinetics; D015345:Oligonucleotide Probes; D010758:Phosphorus / Q000378:metabolism*; D010933:Plankton / Q000145:classification / Q000254:growth & development* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000145:classification
chemical_list: D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S; D010758:Phosphorus