Reverse line blotting: a new technique for the sensitive detection of tick borne pathogens.
Recently, a new molecular diagnostic technique for tick borne pathogens has been developed and is called Reverse Line Blotting. This paper outlines the basis of this method and then illustrates its use for the analysis of blood samples obtained from Ugandan cattle and buffalo. The sensitivity and the specificity are discussed in relation to the use of this technique in the Maghreb
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Department of Veterinary Parasitology, Institute of Comparative Medicine, University of Glasgow, Bearsden Rd, Glasgow G61 1QH, UK.
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D000818:Animals; D016793:Babesia bovis / Q000235:genetics; D001404:Babesiosis / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D002020:Buffaloes; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D015151:Immunoblotting / Q000379:methods* / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012680:Sensitivity and Specificity; D016796:Theileria annulata / Q000235:genetics; D016797:Theileria parva / Q000235:genetics; D013801:Theileriasis / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D017282:Tick-Borne Diseases / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D014454:Uganda / Q000453:epidemiology
D016054:DNA, Protozoan