title: Expression of kallikrein, bradykinin b2 receptor, and endothelial nitric oxide synthase in placenta in normal gestation, preeclampsia, and placenta accreta.
abstract: In an effort to define the varied expression of three vasoactive markers in the clinical models of normal placenta/ normal invasion (n = 11), preeclampsia/restricted trophoblast invasion (n = 15), and placenta accreta/exaggerated invasion (n = 6), we performed semiquantitative immunohistochemistry for kallikrein, bradykinin B2 receptor, and endothelial nitric oxide synthase (eNOS). In the floating villi, the syncytiotrophoblast expressed more kallikrein in placenta accreta (p < 0.05), than in normal and preeclamptic placentas, while the bradykinin B2 receptor and eNOS were similarly expressed in all groups; in the fetal endothelium, the bradykinin B2 receptor was enhanced in placenta accreta (p < 0.005), but kallikrein and eNOS were similarly expressed in the other two groups. In the extravillous trophoblast, both kallikrein and eNOS expression were higher in placenta accreta (p < 0.001), while the bradykinin B2 receptor signal was only enhanced in preeclampsia (p < 0.05). The presence and localization of kallikrein, the bradykinin B2 receptor, and eNOS in the fetomaternal interface in the three study conditions supports a local role for interrelated vasodilatory/antiaggregating systems. This first report of the variations observed in kallikrein and eNOS in a condition of exaggerated trophoblast invasion supports the participation of vasodilatation in trophoblast migration.
authors.affiliation: Centro de Investigaciones Médicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Jenny
authors.initials: J
authors.lastname: Corthorn
authors.forename: Alfredo A
authors.initials: AA
authors.lastname: Germain
authors.forename: Cecilia
authors.initials: C
authors.lastname: Chacón
authors.forename: Sergio
authors.initials: S
authors.lastname: Rey
authors.forename: Gloria X
authors.initials: GX
authors.lastname: Soto
authors.forename: Carlos D
authors.initials: CD
authors.lastname: Figueroa
authors.forename: Werner
authors.initials: W
authors.lastname: Müller-Esterl
authors.forename: Ignacio
authors.initials: I
authors.lastname: Duarte
authors.forename: Gloria
authors.initials: G
authors.lastname: Valdés
mesh_terms: D002824:Chorionic Villi / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D015870:Gene Expression; D005865:Gestational Age; D006801:Humans; D007610:Kallikreins / Q000378:metabolism* / Q000652:urine; D008431:Maternal-Fetal Exchange; D008954:Models, Biological; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D010920:Placenta / Q000098:blood supply / Q000378:metabolism*; D010921:Placenta Accreta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D011250:Pregnancy Complications, Hematologic / Q000378:metabolism; D011256:Pregnancy Outcome; D043782:Receptor, Bradykinin B2 / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism
chemical_list: D043782:Receptor, Bradykinin B2; D052250:Nitric Oxide Synthase Type III; D007610:Kallikreins