Expression of Helicobacter pylori urease subunit B gene in transgenic rice.
Helicobacter pylori ureB antigen gene was cloned to the 5'-end of gus (beta-glucuronidase) reporter gene between CaMV35S promoter and the octopine synthase (OCS) terminator in the plasmid, pCAMBIA13011. It was then introduced into rice genome by Agrobacterium-mediated transformation. A total of 30 regenerated plants with hygromycin resistance were obtained in the selection media. The putative transgenic individuals were tested for the presence of ureB in the nuclear genome of rice plants by PCR analysis. Expression of ureB gene in rice plants was verified by RT-PCR and Western blot analysis using polyclonal human antiserum for transcription and translation levels respectively. These results provide a basis for further studies on the accumulation level of UreB recombinant protein in transgenic rice and potential utilization of transgenic rice for delivery of edible vaccines against Helicobacter pylori.
Gu
Qing
Q
Department of Biotechnology, Zhejiang Gongshang University, Hangzhou, 310035, China. guqing2002@hotmail.com
Han
Ning
N
Liu
Jianyi
J
Zhu
Muyuan
M
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D001428:Bacterial Vaccines; D016480:Helicobacter pylori / Q000201:enzymology* / Q000276:immunology; D006801:Humans; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D021122:Protein Subunits / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012231:Rhizobium / Q000235:genetics; D014510:Urease / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D022462:Vaccines, Edible
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D022462:Vaccines, Edible; D014510:Urease