[Effect of transforming growth factor alpha on the expression of cyclin E and cyclin D1 in gastric carcinoma cells].
OBJECTIVE
To explore the effect of transforming growth factor alpha (TGFalpha) on the expression of cyclin E and D1 in gastric carcinoma cells.


METHODS
Human gastric adenocarcinoma SGC7901 cells were cultured routinely and synchronized at G(0)/G(1) phase in serum-free RPMI-1640. The percentage of the cells at G(0)/G(1) phase was detected by propidium iodide staining and flow cytometry (FCM), and the synchronized cells were cultured in RPMI-1640 supplemented with 2.5% calf serum and treated with 10, 30, and 50 microg/L TGFalpha for 5 h. The expression of cyclin E and D1 in SGC7901 cells was detected by immunofluorescent staining and FCM.


RESULTS
The percentage of the cells at G(0)/G(1) phase increased from 54% in routine culture to 72% in the serum-free RPMI-1640 culture. TGFalpha treatment of the cells synchronized at G(0)/G(1) phase induced significant increment of cyclin E and D1 expressions (P<0.001), and at the dose of TGFalpha of 50 microg/L, their expressions increased by 25.18% and 27.52%, respectively (P<0.001).


CONCLUSIONS
TGFalpha can increase the expression of cyclin E and D1 in gastric carcinoma cells to promote their cell cycle progress.
Liang
Wei-jiang
WJ
Cancer Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lwj2@nfhoc.com
Zhang
Wan-dai
WD
Chen
Cun-long
CL
Yao
Yong-li
YL
Luo
Rong-cheng
RC
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000096:biosynthesis*; D019927:Cyclin E / Q000096:biosynthesis*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology; D016211:Transforming Growth Factor alpha / Q000494:pharmacology*
D019927:Cyclin E; D016211:Transforming Growth Factor alpha; D019938:Cyclin D1