Repeated fluorescence in situ hybridization by a microwave-enhanced protocol.
A novel re-hybridization protocol for pathology archive sections that uses microwave-assisted fluorescence in situ hybridization (FISH) is described. Stripping the probe from the pathology archive sections with HCl and re-hybridizing with the next probe by intermittent microwave irradiation generated clear signals without background noise. Repeated stripping and hybridization with numerous bacterial artificial chromosome (BAC)-derived probes would identify the profile of genome-wide changes in small lesions on sections.
Kitayama
Yasuhiko
Y
First Department of Pathology, Hamamatsu University School of Medicine, Hamamatsu, Japan.
Igarashi
Hisaki
H
Kozu
Taeko
T
Nagura
Kiyoko
K
Ohashi
Yohei
Y
Sugimura
Haruhiko
H
D002869:Chromosome Aberrations; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008872:Microwaves; D016612:Paraffin Embedding