title: Correlation of p53 gene mutation and expression of P53 protein in cholangiocarcinoma.
abstract: OBJECTIVE
  To characterize the tumor suppressor gene p53 mutations and study the correlation of p53 gene mutation and the expression of P53 protein in cholangiocarcinoma.
  
  
  METHODS
  A total of 36 unselected, frozen samples of cholangiocarcinoma were collected. p53 gene status (exon 5-8) and P53 protein were examined by automated sequencing and immunohistochemical staining, combined with the clinical parameters of patients.
  
  
  RESULTS
  p53 gene mutations were found in 22 of 36 (61.1%) patients. Nineteen of 36 (52.8%) patients were positive for P53 protein expression. There were significant differences in extent of differentiation and invasion between the positive and negative expression of P53 protein. However, there were no significant differences in pathologic parameters between the mutations and non-mutations.
  
  
  CONCLUSIONS
  The alterations of the p53 gene evaluated by DNA sequence analysis is relatively accurate. Expression of P53 protein could not act as an independent index to estimate the prognosis of cholangiocarcinoma.
authors.affiliation: Department of Hepatobiliary Surgery of Yantai Yuhuangding Hospital, Yantai 264000, Shangdong Province, China. liu634@263.net
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Shi-Guang
authors.initials: SG
authors.lastname: Zhu
authors.forename: Xian-Ting
authors.initials: XT
authors.lastname: Zhou
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Su
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xu
authors.forename: Shao-Jun
authors.initials: SJ
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D001650:Bile Duct Neoplasms / Q000235:genetics* / Q000378:metabolism; D001653:Bile Ducts, Intrahepatic; D018281:Cholangiocarcinoma / Q000235:genetics* / Q000378:metabolism; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics; D005091:Exons / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009361:Neoplasm Invasiveness / Q000235:genetics; D011379:Prognosis; D017422:Sequence Analysis, DNA; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
chemical_list: D004273:DNA, Neoplasm; D016159:Tumor Suppressor Protein p53