title: Rupture of Reissner's membrane during acute endolymphatic hydrops in the guinea pig: a model for Ménière's disease?
abstract: CONCLUSIONS
  The changes in cochlear function during a destructive acute endolymphatic hydrops were relatively small. This might be consistent with the hypothesis that an endolymphatic hydrops is a marker of disordered inner ear homeostasis rather than the cause of the clinical symptoms of Ménière's disease.
  
  
  OBJECTIVE
  Assessment of cochlear function during induction of a destructive acute endolymphatic hydrops.
  
  
  METHODS
  During repetitive microinjections of 0.5 microl of artificial endolymph at a rate of 50 nl/s the 2f1-f2 and f2-f1 cochlear microphonics distortion products (CMDP) and 2f1-f2 distortion products otoacoustic emissions (DPOAE) were recorded in the guinea pig.
  
  
  RESULTS
  A 'catastrophe' occurred in the inner ear when 2.5-3.5 microl of artificial endolymph was injected. A rupture of Reissner's membrane was then found, most often in the apical turn of the cochlea. This rupture had only minor effects on the endocochlear potential, whereas it caused a marked decrease in 2f1-f2 DPOAE amplitude. The 2f1-f2 and f2-f1 CMDP amplitude increased during each injection prior to the rupture. After the rupture the f2-f1 CMDP amplitude decreased during each injection, possibly due to a shift of the cochlear transducer operating point position.
authors.affiliation: Department of Otorhinolaryngology, University Medical Center Groningen, Groningen, The Netherlands. w.l.valk@kno.umcg.nl
authors.forename: W L
authors.initials: WL
authors.lastname: Valk
authors.forename: H P
authors.initials: HP
authors.lastname: Wit
authors.forename: F W J
authors.initials: FW
authors.lastname: Albers
mesh_terms: D000818:Animals; D003051:Cochlea / Q000503:physiopathology; D003053:Cochlear Duct / Q000293:injuries*; D003055:Cochlear Microphonic Potentials / Q000502:physiology; D004195:Disease Models, Animal; D004710:Endolymph / Q000502:physiology; D018159:Endolymphatic Hydrops / Q000209:etiology / Q000503:physiopathology*; D006168:Guinea Pigs; D008575:Meniere Disease / Q000209:etiology* / Q000503:physiopathology*; D008845:Microinjections; D017084:Otoacoustic Emissions, Spontaneous / Q000502:physiology; D011312:Pressure; D012421:Rupture