title: Coordination of DNA synthesis and replicative unwinding by the S-phase checkpoint pathways.
abstract: The process of DNA replication includes duplex unwinding, followed immediately by DNA synthesis. In eukaryotes, DNA synthesis is disturbed in damaged DNA regions, in replication slow zones, or as a result of insufficient nucleotide level. This review aims to discuss the mechanisms that coordinate DNA unwinding and synthesis, allowing replication to be completed even in the presence of genomic insults. There is a growing body of evidence which suggests that S-phase checkpoint pathways regulate both replicative unwinding and DNA synthesis, to synchronize the two processes, thus ensuring genome stability.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Sciences, 21 G. Bonchev Strasse, Sofia 1113, Bulgaria.
authors.forename: Marina N
authors.initials: MN
authors.lastname: Nedelcheva-Veleva
authors.forename: Dragomir B
authors.initials: DB
authors.lastname: Krastev
authors.forename: Stoyno S
authors.initials: SS
authors.lastname: Stoynov
mesh_terms: D000818:Animals; D004247:DNA / Q000096:biosynthesis; D004265:DNA Helicases / Q000378:metabolism / Q000502:physiology; D004261:DNA Replication / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins; D017346:Protein Serine-Threonine Kinases; D016196:S Phase / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014981:Xenopus
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; C088937:MEC1 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; D004265:DNA Helicases