Characterization of enantioselective binding of racemic natural tetrahydropalmatine to DNA by chromatographic methods.
A racemate from natural product, tetrahydropalmatine (THP), was characterized on its enantioselective binding to DNA by the chromatographic methods including microdialysis/HPLC, centrifugal ultrifiltration/HPLC and immobilized DNA affinity chromatography. It was found that its (+)-enantiomer was preferential to binding on B-form duplex DNA including calf thymus DNA, AT and GC sequence oligo DNA, as well as triplex oligo DNA. The binding constants of the THP enantiomers to ct-DNA were determined with the methods of microdialysis/HPLC and frontal affinity chromatography. In addition, the DNA structural preference of either enantiomer was evaluated with the chromatographic methods.
Su
Xingye
X
National Chromatographic R. & A. Centre, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Qin
Feng
F
Kong
Liang
L
Ou
Junjie
J
Xie
Chuanhui
C
Zou
Hanfa
H
D001600:Berberine Alkaloids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D017551:Microdialysis; D013237:Stereoisomerism; D014461:Ultracentrifugation
D001600:Berberine Alkaloids; C014215:tetrahydropalmatine; D004247:DNA