title: Susceptibility of red blood cell lipids to in vitro oxidation and antioxidant status in preeclampsia.
abstract: OBJECTIVE
  To investigate susceptibility of red blood cell (RBC) lipids to oxidation and antioxidant status in preeclampsia.
  
  
  METHODS
  Twenty-one women with mild preeclampsia, 21 women with severe preeclampsia, and 20 healthy pregnant women were included in this cross-sectional study. Susceptibility of RBC to oxidative stress was determined by measuring RBC-malondialdehyde levels after incubation with hydrogen peroxide. Vitamins E and C, total carotenoids and erythrocyte superoxide dismutase and glutathione peroxidase (GPx) activities and serum total antioxidant capacity (TAC) were determined spectrophotometrically. One-way analysis of variance and correlation analysis were used for statistical analyses.
  
  
  RESULTS
  Compared with the normal pregnant women, susceptibility of RBC to oxidation was enhanced in the mild (p<0.05) and severe (p<0.01) preeclampsia groups, TAC was lower in the mild (p<0.01) and severe (p<0.001) preeclampsia groups. Vitamin C level was decreased in severe preeclampsia and total carotene level was decreased in mild and severe preeclampsia groups (p<0.05). GPx activity was also decreased in the mild (p<0.01) and severe (p<0.05) preeclampsia groups.
  
  
  CONCLUSIONS
  The results of the present study supported the oxidative stress hypothesis of preeclampsia and it is possible that RBC play a role in the pathophysiology of the disease.
authors.affiliation: Department of Biochemistry, Medical Faculty, Uludag University, 16059 Görükle-Bursa, Turkey. mdirican@uludag.edu.tr
authors.forename: Melahat
authors.initials: M
authors.lastname: Dirican
authors.forename: Ozlem
authors.initials: O
authors.lastname: Safak
authors.forename: Gürkan
authors.initials: G
authors.lastname: Uncu
authors.forename: Emre
authors.initials: E
authors.lastname: Sarandöl
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D018384:Oxidative Stress; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D055815:Young Adult
chemical_list: D000975:Antioxidants