Temperature as a variable in liquid chromatography: development and application of a model for the separation of model drugs using water as the eluent.
The use of high temperatures in liquid chromatography allows for the use of a purely aqueous mobile phase. At elevated temperatures water possesses many of the characteristics of organic solvents in terms of eluotropic strength, as well as having a lower viscosity. A model is developed, based on data obtained using a range of model drugs, which demonstrates the relationship between temperature, flow and pressure. Experimental data from different column types, at temperatures from 40 degrees C to 180 degrees C, is presented which matches well with the predicted data from the model.
Edge
A M
AM
Department of Drug Metabolism and Pharmacokinetics, AstraZeneca Pharmaceuticals, Alderley Park, Macclesfield, Cheshire SK10 4TG, UK. anthonye@astrazeneca.com <anthonye@astrazeneca.com>
Shillingford
S
S
Smith
C
C
Payne
R
R
Wilson
I D
ID
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis; D013696:Temperature; D014867:Water / Q000737:chemistry*
D004364:Pharmaceutical Preparations; D014867:Water