title: Gel-free analysis of the human brain proteome: application of liquid chromatography and mass spectrometry on biopsy and autopsy samples.
abstract: This paper reports on the findings of the Biomedical Research Institute, as one of the participants in the pilot study of the HUPO Brain Proteome Project. A biopsy and autopsy study sample derived from human brain was distributed among the participants for proteomic analysis. In our laboratory, attention was focused on protein identification using the bottom-up shotgun approach. Protein extracts derived from both samples were trypsinized and analyzed separately by 2-D LC and MS. In a complementary approach, the tryptic digests were analyzed directly by LC-ESI-MS/MS and gas-phase fractionation in the mass spectrometer. Taken together, both proteomic approaches in combination with a stringent evaluation process, resulted in the confident identification of 209 proteins in the human brain samples under investigation.
authors.affiliation: Hasselt University, Biomedical Research Institute and transnationale Universiteit Limburg, School of Life Sciences, Diepenbeek, Belgium.
authors.forename: Debora
authors.initials: D
authors.lastname: Dumont
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Noben
authors.forename: Peter
authors.initials: P
authors.lastname: Verhaert
authors.forename: Piet
authors.initials: P
authors.lastname: Stinissen
authors.forename: Johan
authors.initials: J
authors.lastname: Robben
mesh_terms: D000328:Adult; D001344:Autopsy; D001706:Biopsy; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002853:Chromatography, Liquid; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology; D020543:Proteome / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D020543:Proteome