title: Phylogenetic diversity of planktonic archaea in the estuarine region of East China Sea.
abstract: To examine the diversity and structure of archaeal communities in the Yangtze River estuarine region of East China Sea (ECS), the 16S rRNA gene clone libraries of two typical sites were constructed with the archaea-specific primers. In total, 71 clones randomly selected were screened by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis and 21 clones with unique RFLP pattern were sequenced. All the sequences are clustered into the two groups of Marine Group I (MGI) and Marine Group II (MGII) which are affiliated with the phyla Crenarchaeota and Euryarchaeota, respectively. MGI clones dominate both libraries with 20 MGI sequences obtained. The majority of sequences are closely related to uncultured marine archaea except for two sequences of which the nearest neighbor is a newly identified isolate of nitrifying marine archaeon Nitrosopumilus maritimus (98% identity). The results indicate that ECS coastal waters are inhabited by archaeal community with low dominance and high diversity corresponding to the complex estuarine environments, suggesting that archaea may perform an important role in the estuarine ecosystem.
authors.affiliation: National Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen 361005, PR China. zengyh@xmu.edu.cn
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Zeng
authors.forename: Heyang
authors.initials: H
authors.lastname: Li
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Jiao
mesh_terms: D044822:Biodiversity; D002681:China; D019615:Crenarchaeota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D019641:DNA, Archaeal / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D019605:Euryarchaeota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S