Characterisation of an isolate of Narcissus degeneration virus from Chinese narcissus (Narcissus tazetta var. chinensis).
A potyvirus from Chinese narcissus was transmitted mechanically to three species of Narcissus and to Lycoris radiata but not to 22 other test species. In western blot, the coat protein reacted strongly with Narcissus degeneration virus (UK isolate) antiserum. Antiserum raised to the Chinese virus did not react with eighteen other potyviruses. The complete nucleotide sequence (9816 nt) had the typical genome organisation for a member of the genus Potyvirus. Sequence comparisons and phylogenetic analysis showed that the Chinese virus was different from all previously sequenced potyviruses but distantly related to onion yellow dwarf and shallot yellow stripe viruses.
Chen
J
J
Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, PR China. jchen1975@zjip.com
Shi
Y-H
YH
Adams
M J
MJ
Zheng
H-Y
HY
Qin
B-X
BX
Chen
J-P
JP
D000595:Amino Acid Sequence; D001483:Base Sequence; D036022:Capsid Proteins / Q000302:isolation & purification / Q000648:ultrastructure; D003062:Codon; D016679:Genome, Viral; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure; D008969:Molecular Sequence Data; D008970:Molecular Weight; D031427:Narcissus / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014771:Virion / Q000302:isolation & purification / Q000648:ultrastructure
D036022:Capsid Proteins; D003062:Codon