[Genetic diversity of Beauveria bassiana (Bals.) Vuill. in forest ecosystem assessed by inter-simple sequence repeat (ISSR) markers].
In the present paper, the genetic diversity of 48 Beauveria bassiana strains from different altitudes and at different seasons in Dabie Mountains of western Anhui was estimated using inter-simple sequence repeat (ISSR) markers. Twelve among 33 ISSR primers were chosen for their reproducibility and high polymorphism. Seven (2 - 11) markers per primer were scored, and a total of 84 fragments were amplified, in which 73 (81%) were polymorphic. Genetic diversity analysis revealed a relatively high level of intraspecific genetic diversity of B. bassiana in Dabie Mountains of western Anhui: the percentage of polymorphic loci (PPL) was 81%, Nei's genetic diversity (He) was 0.3187 and Shannon's genetic diversity index (I) was 0.4782. The genetic differentiation, Gst was 0.1028, indicating that a low degree of genetic differentiation occurred in the B. Bassiana among populations.
Li
Min
M
Anhui Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, China. limincxl@gmail.com
Wang
Si-Bao
SB
Fan
Mei-Zhen
MZ
Li
Zeng-Zhi
ZZ
Huang
Yong-Ping
YP
D000531:Altitude; D052982:Beauveria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002681:China; D017753:Ecosystem; D014644:Genetic Variation; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012621:Seasons; D014197:Trees / Q000382:microbiology*