title: Adhesion of Escherichia coli K88ab fimbriae to porcine enterocytes: effect on enzymatic activities of the brush border, and cyclic AMP and GMP levels.
abstract: We have studied the cellular alterations, after in vitro adherence, of purified K88ab fimbriae to membranes of porcine enterocytes. Effects on enzymatic activities as disaccharydases and alkaline phosphatase show low changes. While cAMP levels were decreased (44%), guanylyl cyclase was increased (up to 200%), and levels of cGMP were in consequence significantly affected. This study support the role of cyclic GMP as intracellular mediator for adherence, and suggest their implication in disease, affecting a membrane-mediated mechanisms for guanylate cyclase activation, that is unique in the intestine.
authors.affiliation: Departamento de Sanidad Animal, Universidad de León, Leon, Spain.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Caloca
authors.forename: Susana
authors.initials: S
authors.lastname: Suárez
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism; D001422:Bacterial Adhesion / Q000502:physiology; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D020895:Enterocytes / Q000378:metabolism / Q000382:microbiology*; D004926:Escherichia coli / Q000378:metabolism / Q000502:physiology*; D004927:Escherichia coli Infections / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000378:metabolism; D036781:Fimbriae Proteins / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006162:Guanylate Cyclase / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology; D008871:Microvilli / Q000201:enzymology / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000378:metabolism / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; D036781:Fimbriae Proteins; D000242:Cyclic AMP; D006162:Guanylate Cyclase; D006152:Cyclic GMP