title: Methicillin-resistant S. aureus infections among patients in the emergency department.
abstract: BACKGROUND
  Methicillin-resistant Staphylococcus aureus (MRSA) is increasingly recognized in infections among persons in the community without established risk factors for MRSA.
  
  
  METHODS
  We enrolled adult patients with acute, purulent skin and soft-tissue infections presenting to 11 university-affiliated emergency departments during the month of August 2004. Cultures were obtained, and clinical information was collected. Available S. aureus isolates were characterized by antimicrobial-susceptibility testing, pulsed-field gel electrophoresis, and detection of toxin genes. On MRSA isolates, we performed typing of the staphylococcal cassette chromosome mec (SCCmec), the genetic element that carries the mecA gene encoding methicillin resistance.
  
  
  RESULTS
  S. aureus was isolated from 320 of 422 patients with skin and soft-tissue infections (76 percent). The prevalence of MRSA was 59 percent overall and ranged from 15 to 74 percent. Pulsed-field type USA300 isolates accounted for 97 percent of MRSA isolates; 74 percent of these were a single strain (USA300-0114). SCCmec type IV and the Panton-Valentine leukocidin toxin gene were detected in 98 percent of MRSA isolates. Other toxin genes were detected rarely. Among the MRSA isolates, 95 percent were susceptible to clindamycin, 6 percent to erythromycin, 60 percent to fluoroquinolones, 100 percent to rifampin and trimethoprim-sulfamethoxazole, and 92 percent to tetracycline. Antibiotic therapy was not concordant with the results of susceptibility testing in 100 of 175 patients with MRSA infection who received antibiotics (57 percent). Among methicillin-susceptible S. aureus isolates, 31 percent were USA300 and 42 percent contained pvl genes.
  
  
  CONCLUSIONS
  MRSA is the most common identifiable cause of skin and soft-tissue infections among patients presenting to emergency departments in 11 U.S. cities. When antimicrobial therapy is indicated for the treatment of skin and soft-tissue infections, clinicians should consider obtaining cultures and modifying empirical therapy to provide MRSA coverage.
authors.affiliation: Department of Emergency Medicine, Olive View-UCLA Medical Center, Sylmar, Calif 91342, USA. idnet@ucla.edu
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Moran
authors.forename: Anusha
authors.initials: A
authors.lastname: Krishnadasan
authors.forename: Rachel J
authors.initials: RJ
authors.lastname: Gorwitz
authors.forename: Gregory E
authors.initials: GE
authors.lastname: Fosheim
authors.forename: Linda K
authors.initials: LK
authors.lastname: McDougal
authors.forename: Roberta B
authors.initials: RB
authors.lastname: Carey
authors.forename: David A
authors.initials: DA
authors.lastname: Talan
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004636:Emergency Service, Hospital; D005098:Exotoxins / Q000235:genetics; D005260:Female; D006801:Humans; D007956:Leukocidins; D008297:Male; D016106:Methicillin Resistance / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D046915:Penicillin-Binding Proteins; D018461:Soft Tissue Infections / Q000188:drug therapy / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology*; D013207:Staphylococcal Skin Infections / Q000188:drug therapy / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D014481:United States
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus