title: Association of H pylori cagA and vacA genotypes and IL-8 gene polymorphisms with clinical outcome of infection in Iranian patients with gastrointestinal diseases.
abstract: OBJECTIVE
  To find out if a functional promoter polymorphism in the IL-8 gene along with cagA status and polymorphisms in vacA gene influence the type of diseases in Iranian patients infected by H pylori.
  
  
  METHODS
  IL-8 -251 A/T polymorphism was genotyped by oligonucleotide allele specific PCR (ASO-PCR) in a sample of 233 patients with H pylori infection undergoing upper gastrointestinal endoscopy. The presence of cagA gene and polymorphisms in vacA gene was also determined by PCR. Association of these genetic polymorphisms with the development of gastritis, peptic ulcers as well as gastric cancer was tested.
  
  
  RESULTS
  When the patients with different clinical manifestations were compared according to the presence of cagA gene or various vacA genotypes, only the vacA genotypes were significantly different among gastritis, peptic ulcer and gastric cancer patients (chi 2 = 17.8; P = 0.001). Furthermore, there was a significant difference in the frequency of IL-8 -251 A/T genotypes between patients with gastric cancer and benign diseases (chi 2 = 10.47; P = 0.005).
  
  
  CONCLUSIONS
  The IL-8 -251 A/T polymorphism and the polymorphisms in H pylori vacA gene are involved in limiting the infection outcome to gastritis and peptic ulcer or in favoring cancer onset in Iranian patients.
authors.affiliation: Department of Immunology and Autoimmune Diseases Research Center, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran. immunol2@sums.ac.ir
authors.forename: Eskandar
authors.initials: E
authors.lastname: Kamali-Sarvestani
authors.forename: Abdulah
authors.initials: A
authors.lastname: Bazargani
authors.forename: Malihe
authors.initials: M
authors.lastname: Masoudian
authors.forename: Kamran
authors.initials: K
authors.lastname: Lankarani
authors.forename: Ali-Reza
authors.initials: AR
authors.lastname: Taghavi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Saberifiroozi
mesh_terms: D000328:Adult; D000483:Alleles; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D005260:Female; D005767:Gastrointestinal Diseases / Q000382:microbiology*; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D007492:Iran; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016896:Treatment Outcome
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016209:Interleukin-8; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori