title: eIF5A binds to translational machinery components and affects translation in yeast.
abstract: The putative translation factor eIF5A is essential for cell viability and is highly conserved from archebacteria to mammals. Although this protein was originally identified as a translation initiation factor, subsequent experiments did not support a role for eIF5A in general translation. In this work, we demonstrate that eIF-5A interacts with structural components of the 80S ribosome, as well as with the translation elongation factor 2 (eEF2). Moreover, eIF5A is further shown to cofractionate with monosomes in a translation-dependent manner. Finally, eIF5A mutants show altered polysome profiles and are sensitive to translation inhibitors. Our results re-establish a function for eIF5A in translation and suggest a role for this factor in translation elongation instead of translation initiation.
authors.affiliation: Department of Biological Sciences, School of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, SP 14801-902, Brazil.
authors.forename: Cleslei F
authors.initials: CF
authors.lastname: Zanelli
authors.forename: Ana L C
authors.initials: AL
authors.lastname: Maragno
authors.forename: Ana P B
authors.initials: AP
authors.lastname: Gregio
authors.forename: Suzanne
authors.initials: S
authors.lastname: Komili
authors.forename: José R
authors.initials: JR
authors.lastname: Pandolfi
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Mestriner
authors.forename: Wilton R
authors.initials: WR
authors.lastname: Lustri
authors.forename: Sandro R
authors.initials: SR
authors.lastname: Valentini
mesh_terms: D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D009154:Mutation; D010441:Peptide Chain Elongation, Translational; D010448:Peptide Initiation Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D008239:Lysine; D010448:Peptide Initiation Factors; D011500:Protein Synthesis Inhibitors; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A; C100028:hypusine