Inefficient antigen presentation via the IgA Fc receptor (FcalphaRI) on dendritic cells.
Dendritic cells (DC) are professional antigen presenting cells that can induce and regulate adaptive immune responses. For that reason, DC are attractive candidates for vaccination strategies. Recently, expression of the IgA Fc receptor (FcalphaRI, CD89) was observed on DC, which activation led to DC maturation. We have investigated the potential of DC FcalphaRI as a target molecule for vaccination against cancer. FcalphaRI expression was observed on human blood myeloid DC. Furthermore, expression of FcalphaRI was low on immature DC, cultured from either human monocytes or FcalphaRI transgenic (Tg) mouse bone marrow cells. Addition of TNF-alpha to culture regimes of both human and mouse DC led to more semi-mature DC, on which FcalphaRI expression was slightly upregulated. FcalphaRI on both human and FcalphaRI Tg mouse DC was internalized after receptor crosslinking. Antigen presentation, measured in FcalphaRI Tg mouse DC, was however minimal. As antigen presentation is crucial to elicit effective T cell responses, these data suggest that targeting of DC FcalphaRI is not optimal for DC vaccination strategies.
Otten
Marielle A
MA
Immunotherapy Laboratory, Department of Immunology, UMC, Utrecht, The Netherlands.
Groenveld
Irene
I
van de Winkel
Jan G J
JG
van Egmond
Marjolein
M
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D015703:Antigens, CD / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D011961:Receptors, Fc / Q000235:genetics / Q000502:physiology*
D015703:Antigens, CD; C036832:Fc(alpha) receptor; D007070:Immunoglobulin A; D011961:Receptors, Fc