title: Genetic variability of bovine viral diarrhoea virus subtypes at 3'-nontranslated region.
abstract: Ten isolates belonging to different BVDV-1 subtypes and three BVDV-2 isolates were studied in 3'-nontranslated region (3'-NTR) by sequencing of PCR products and comparative computer-assisted nucleotide sequence analysis. The alignment of nucleotide sequences revealed that all BVDV-1 isolates except BVDV-1a isolates represented by NADL strain were characterised with a deletion of 38 nucleotides in the variable region located after the stop codon. For all BVDV-1 subtypes, a constant region at the end of viral genome was highly conserved. The variable and constant regions with no significant insertions or deletions were also identified in BVDV-2 isolates. The poly AT reach region was situated at different locations in both pestiviruses.
authors.affiliation: Department of Infectious Diseases and Parasitology, University of Veterinary Medicine, Komenskeho 73, 041 81, Kosice, Slovakia. vilcek@uvm.sk
authors.forename: S
authors.initials: S
authors.lastname: Vilcek
authors.forename: Mária
authors.initials: M
authors.lastname: Kolesárová
authors.forename: Anna
authors.initials: A
authors.lastname: Jacková
mesh_terms: D020413:3' Untranslated Regions / Q000032:analysis*; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000235:genetics* / Q000302:isolation & purification; D030242:Diarrhea Virus 2, Bovine Viral / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D030243:Hemorrhagic Syndrome, Bovine / Q000821:virology; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral