title: Production of Bacillus sphaericus entomopathogenic biomass using brewery residues.
abstract: The use of brewery residues--yeast and trub--has been evaluated aiming to minimize the costs of the industrial production of Bacillus sphaericus-based bioinsecticide. Both brewery residues promoted growth and sporulation of the three B. sphaericus strains that were isolated from Brazilian soils (S1, S2, and S20). However, distinct growth and sporulation behaviors were observed in relation to the different nutritional conditions and strain used. The maximum sporulation percentage was obtained through the cultivation of S20 strain in brewery residual yeast. In general, the entomopathogenic biomasses produced showed good results for toxicity to Culex larvae. The minimum values of larvae population (LC50) were observed for the S20 strain grown on yeast brewery residue-containing media. After fermentation, a considerable decrease in the organic material of alternative media was verified, although the residual values were still higher than that considered appropriate for effluent discharge.
authors.affiliation: Universidade Federal do Rio de Janeiro, Escola de Química, Centro de Tecnologia, Bloco E, Ilha do Fundão, 21949-900 Rio de Janeiro, RJ, Brazil.
authors.forename: Cristiane Darco Cruz
authors.initials: CD
authors.lastname: Martins
authors.forename: Paula Fernandes
authors.initials: PF
authors.lastname: De Aguiar
authors.forename: Eliana Flavia Camporese
authors.initials: EF
authors.lastname: Sérvulo
mesh_terms: D000818:Animals; D001407:Bacillus / Q000502:physiology*; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000096:biosynthesis*; D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods*; D003465:Culex / Q000187:drug effects / Q000382:microbiology*; D019649:Food Industry / Q000379:methods; D007220:Industrial Waste / Q000517:prevention & control*; D010572:Pest Control, Biological / Q000379:methods*; D012722:Sewage / Q000382:microbiology*; D012988:Soil Microbiology
chemical_list: D001427:Bacterial Toxins; D007220:Industrial Waste; D012722:Sewage; C046134:binB protein, Bacillus sphaericus