title: Comparison between one- and two-hemorrhage models of cerebral vasospasm in rabbits.
abstract: Injection of blood into the cisterna magna is one of the most frequently used methods to produce subarachnoid hemorrhage (SAH) models in animals. Although the two-hemorrhage model of vasospasm is frequently used in canine and rat models, most studies with rabbits only use the one-hemorrhage model. In the present study, we accomplished a side-by-side comparison between one- and two-hemorrhage models in rabbits. A total of 38 rabbits were randomly divided into three groups, i.e. control group (n = 5), one (n = 15)- and two (n = 18)-hemorrhage model groups. The degree of cerebral vasospasm, the time course of cerebral vasospasm, the clinical behavior, and the residual amount of subarachnoid blood clots were measured on days 3, 5 and 7 after the establishment of the models. Compared with one-hemorrhage model, the time course of vasospasm in the two-hemorrhage model was more coincident with that observed in humans, produced more severe vasospasm after SAH, and had an acceptable low mortality. In conclusion, the two-hemorrhage model in rabbits is more appropriate than the one-hemorrhage model for the research on SAH or cerebral vasospasm, and thus can be used for the investigation of the mechanisms of and therapeutic approaches for cerebral vasospasm.
authors.affiliation: Department of Neurosurgery, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing 210002, Jiangsu Province, PR China.
authors.forename: Meng-Liang
authors.initials: ML
authors.lastname: Zhou
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Shi
authors.forename: Jia-Quan
authors.initials: JQ
authors.lastname: Zhu
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Hang
authors.forename: Lei
authors.initials: L
authors.lastname: Mao
authors.forename: Ke-Fei
authors.initials: KF
authors.lastname: Chen
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Yin
mesh_terms: D000818:Animals; D001488:Basilar Artery / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D011817:Rabbits; D012720:Severity of Illness Index; D013345:Subarachnoid Hemorrhage / Q000401:mortality / Q000473:pathology / Q000503:physiopathology*; D015996:Survival Rate; D020301:Vasospasm, Intracranial / Q000401:mortality / Q000473:pathology / Q000503:physiopathology*