Quantification of cDNA generated by reverse transcription of total RNA provides a simple alternative tool for quantitative RT-PCR normalization.
Libus
Jirí
J
Institute of Experimental Botany, Prague, Czech Republic. libus@ueb.cas.cz
Storchová
Helena
H
D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D005456:Fluorescent Dyes / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048348:Reverse Transcription; D012680:Sensitivity and Specificity
D018076:DNA, Complementary; D005456:Fluorescent Dyes; D012333:RNA, Messenger; D012313:RNA