Detection of fatty acid beta-oxidizing syntrophic bacteria by fluorescence in situ hybridization.
A new 16S rRNA-targeted oligonucleotide probe, specific for the cluster of fatty acid beta-oxidizing syntrophic bacteria of the family Syntrophomonadaceae was designed for fluorescence in situ hybridization. This probe was evaluated with target as well as non-target cultures. Moreover this probe was assessed with butyrate and oleate degrading enrichment cultures and methanogenic sludges from full-scale plants. The results showed that the probe revealed the presence of fatty acid beta-oxidizing syntrophic bacteria in some of the samples analyzed. However, cell quantification was possible only in enrichment cultures and in a flocculent sludge from a reactor that treats lipid-rich wastewaters, but not in methanogenic granular sludges from upflow anaerobic sludge blanket reactors.
Menes
R J
RJ
Cátedra de Microbiologia, Facultad de Química y Facultad de Ciencias, UDELAR, Montevideo, Uruguay. jmenes@fq.edu.uy
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D001483:Base Sequence; D003013:Clostridium / Q000302:isolation & purification* / Q000378:metabolism; D017931:DNA Primers; D005227:Fatty Acids / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D010084:Oxidation-Reduction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D017931:DNA Primers; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S