[Chemokine CXCL16 induces proliferation and invasion of human first-trimester cytotrophoblasts in autocrine manner].
To investigate the secretion and autocrine roles of chemokine CXCL16 in first-trimester human cytotrophoblast cells, enzyme-linked immunosorbent assay (ELISA) was used to detect the CXCL16 secretion of primary-cultured cytotrophoblast cells in various lengths of culture time and various plated densities in vitro. Then, the [3H] thymidine incorporation assay and matrigel invasion assay were applied respectively to investigate proliferation and invasion roles of CXCL16 on the cytotrophoblast cells treated with rhCXCL16. Our results showed that the primary cultured cytotrophoblasts were found to secrete in vitro CXCL16 spontaneously and continuously. CXCL16 stimulated the proliferation and invasion of cytotrophoblast cells in vitro. These results indicated that CXCL16 is secreted in first-trimester cytotrophoblast cells and induces the proliferation and invasion of cytotrophoblast cells in autocrine manner. CXCL16 plays important roles in human placentation and extravillous trophoblast invasiveness.
Huang
Yu
Y
Lab. for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, Shanghai Medical College, Fudan University, Shanghai 200011.
Li
Da Jin
DJ
D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D019743:Chemokines, CXC / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
D019743:Chemokines, CXC; D011994:Recombinant Proteins