Evaluation of cellulase preparations for hydrolysis of hardwood substrates.
Seven cellulase preparations from Penicillium and Trichoderma spp. were evaluated for their ability to hydrolyze the cellulose fraction of hardwoods (yellow poplar and red maple) pretreated by organosolv extraction, as well as model cellulosic substrates such as filter paper. There was no significant correlation among hydrolytic performance on pretreated hardwood, based on glucose release, and filter paper activity. However, performance on pretreated hardwood showed significant correlations to the levels of endogenous beta-glucosidase and xylanase activities in the cellulase preparation. Accordingly, differences in performance were reduced or eliminated following supplementation with a crude beta-glucosidase preparation containing both activities. These results complement a previous investigation using softwoods pretreated by either organosolv extraction or steam explosion. Cellulase preparations that performed best on hardwood also showed superior performance on the softwood substrates.
Berlin
Alex
A
Forest Products Biotechnology, Faculty of Forestry, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada. alex.berlin@ubc.ca
Gilkes
Neil
N
Kilburn
Douglas
D
Maximenko
Vera
V
Bura
Renata
R
Markov
Alexander
A
Skomarovsky
Anton
A
Gusakov
Alexander
A
Sinitsyn
Arkady
A
Okunev
Oleg
O
Solovieva
Irina
I
Saddler
John N
JN
D002480:Cellulase / Q000737:chemistry* / Q000145:classification*; D002482:Cellulose / Q000737:chemistry*; D003198:Computer Simulation; D004789:Enzyme Activation; D006868:Hydrolysis; D007700:Kinetics; D008954:Models, Biological; D008956:Models, Chemical; D013379:Substrate Specificity; D014197:Trees / Q000737:chemistry*; D014934:Wood
D002482:Cellulose; D002480:Cellulase