A study of the relative dominance of selected anaerobic sulfate-reducing bacteria in a continuous bioreactor by fluorescence in situ hybridization.
The diversity and the community structure of sulfate-reducing bacteria (SRB) in an anaerobic continuous bioreactor used for treatment of a sulfate-containing wastewater were investigated by fluorescence in situ hybridization. Hybridization to the 16S rRNA probe EUB338 for the domain Bacteria was performed, followed by a nonsense probe NON338 as a control for nonspecific staining. Sulfate-reducing consortia were identified by using five nominally genus-specific probes (SRB129 for Desulfobacter, SRB221 for Desulfobacterium, SRB228 for Desulfotomaculum, SRB660 for Desulfobulbus, and SRB657 for Desulfonema) and four group-specific probes (SRB385 as a general SRB probe, SRB687 for Desulfovibrioaceae, SRB814 for Desulfococcus group, and SRB804 for Desulfobacteriaceae). The total prokaryotic population was determined by 4',6-diamidino-2-phenylindole staining. Hybridization analysis using these 16S rRNA-targeted oligonucleotide probes showed that, of those microbial groupings investigated, Desulfonema, Desulfobulbus, spp., and Desulfobacteriaceae group were the main sulfate-reducing bacteria in the bioreactor when operated at steady state at 35 degrees C, pH 7.8, and a 2.5-day residence time with feed stream containing 2.5 kg m-3 sulfate as terminal electron acceptor and 2.3 kg m-3 acetate as carbon source and electron donor.
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Bioprocess Engineering Research Unit, Department of Chemical Engineering, University of Cape Town, Rondebosch 7701, Cape Town, South Africa. bulent.icgen@tubitak.gov.tr
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D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D019149:Bioreactors / Q000382:microbiology*; D015169:Colony Count, Microbial; D003470:Culture Media; D020564:Deltaproteobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016999:Sulfur-Reducing Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification
D003470:Culture Media; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S