title: Extense variant gene family repertoire overlap in Western Amazon Plasmodium falciparum isolates.
abstract: In order to find a molecular basis for observations of relatively fast developing immunity to malarial infections in the Western Amazon region, the partial var, stevor and rif gene repertoires of nine different Plasmodium falciparum isolates collected in 1985 and 2000-2004 were evaluated. In contrast to previous results from South East Asia, the variant gene repertoire in Brazilian isolates is rather small and redundant. While the individual var repertoire sizes of Brazilian strains did not differ from Southeast Asian/African isolates, we found an over three times higher overlap of var sequence repertoires in Amazonian strains which was also conserved over time, suggesting the ongoing circulation of a similar var gene repertoire. Coincidently, almost 40% of the sequences identified herein showed the highest degree of similarity to var genes from either Brazilian or Venezuelan isolates, indicating a limited var repertoire of P. falciparum in the Amazon Basin as a whole. The intrastrain similarities of var genes were slightly but significantly lower than in Southeast Asian/African samples suggesting a higher selective pressure for diversification in Amazonian isolates. Despite of higher copy numbers per genome, rif genes also showed a significant repertoire overlap. stevor genes, which share the same predominant subtelomeric localization as var and rif genes, showed a still higher repertoire overlap and were highly similar to 3D7 stevor genes, indicating stronger functional conservation than var and rif genes. This is the first study that reveals that P. falciparum variant gene repertoires of certain areas can be limited. This has important implications for the strain-specific immunity against variant antigens occurring in these areas.
authors.affiliation: Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Letusa
authors.initials: L
authors.lastname: Albrecht
authors.forename: Emilio Fernando
authors.initials: EF
authors.lastname: Merino
authors.forename: Erika H E
authors.initials: EH
authors.lastname: Hoffmann
authors.forename: Marcelo U
authors.initials: MU
authors.lastname: Ferreira
authors.forename: Ricardo G
authors.initials: RG
authors.lastname: de Mattos Ferreira
authors.forename: Ana Lucia
authors.initials: AL
authors.lastname: Osakabe
authors.forename: Rosimeire C
authors.initials: RC
authors.lastname: Dalla Martha
authors.forename: Michael
authors.initials: M
authors.lastname: Ramharter
authors.forename: Alan M
authors.initials: AM
authors.lastname: Durham
authors.forename: João Eduardo
authors.initials: JE
authors.lastname: Ferreira
authors.forename: Hernando A
authors.initials: HA
authors.lastname: Del Portillo
authors.forename: Gerhard
authors.initials: G
authors.lastname: Wunderlich
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D001938:Brazil; D004912:Erythrocytes / Q000469:parasitology; D005681:Gabon; D018628:Gene Dosage; D017125:Genes, Protozoan; D018503:Genome, Protozoan; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D000953:Antigens, Protozoan; C474556:STEVOR antigen, Plasmodium falciparum