title: Reproducible improvements in order and diffraction limit of crystals of bovine mitochondrial F(1)-ATPase by controlled dehydration.
abstract: Orthorhombic crystals of bovine F(1)-ATPase have been subjected to controlled dehydration. A decrease in the relative humidity surrounding the crystals to 90% reproducibly reduced their unit-cell volume by 22% (950,000 Angstrom(3)) and improved the diffraction limit and mosaic spread of the crystals significantly. These dehydrated crystals diffracted X-rays to 1.8 Angstrom resolution at a synchrotron source, the best diffraction limit yet attained with these crystals, although radiation damage limited the resolution of a complete data set to 1.95 Angstrom.
authors.affiliation: The Medical Research Council Dunn Human Nutrition Unit, Cambridge, England.
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Bowler
authors.forename: Martin G
authors.initials: MG
authors.lastname: Montgomery
authors.forename: Andrew G W
authors.initials: AG
authors.lastname: Leslie
authors.forename: John E
authors.initials: JE
authors.lastname: Walker
mesh_terms: D000251:Adenosine Triphosphatases; D000818:Animals; D002417:Cattle; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D003681:Dehydration; D008928:Mitochondria / Q000201:enzymology*; D008958:Models, Molecular; D015233:Models, Statistical; D006180:Proton-Translocating ATPases / Q000737:chemistry*; D015203:Reproducibility of Results; D013367:Submitochondrial Particles / Q000737:chemistry; D013997:Time Factors
chemical_list: D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases