title: Expression of autocrine motility factor mRNA is a poor prognostic factor in high-grade astrocytoma.
abstract: It has been reported that tumor infiltration is correlated with the expression of autocrine motility factor (AMF) and its receptor 78 kDa glycoprotein (gp78). The purpose of the present study was to detect AMF and gp78 mRNA expression levels and their localization in high-grade astrocytomas (glioblastoma and anaplastic astrocytoma) and to determine whether AMF and gp78 are important prognostic factors. A total of 32 formalin-fixed and paraffin-embedded glioblastomas and 23 formalin-fixed and paraffin-embedded anaplastic astrocytomas was used. The expressions of AMF and gp78 mRNA were detected using the highly sensitive in situ hybridization method. The expression of AMF mRNA was detected in 27 of 32 glioblastomas (84.4%) and 11 of 23 anaplastic astrocytomas (47.8%). The positivity of AMF mRNA was significantly higher in glioblastomas than in anaplastic astrocytomas (P = 0.0094), but gp78 mRNA was detected in most cases and no statistical significance was observed. The overall survival of patients with AMF expression was significantly shorter than patients without AMF expression (P = 0.0175). In anaplastic astrocytomas, the overall survival of patients with AMF expression was also significantly shorter than in patients without AMF expression (P = 0.0058). This study demonstrated that AMF is a poor prognostic factor in high-grade astrocytomas.
authors.affiliation: Department of Neurosurgery, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan. tanizaki@med.kitasato-u.ac.jp
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Tanizaki
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sato
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Oka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Utsuki
authors.forename: Koji
authors.initials: K
authors.lastname: Kondo
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Miyajima
authors.forename: Ryo
authors.initials: R
authors.lastname: Nagashio
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Fujii
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001254:Astrocytoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis*; D001932:Brain Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D015870:Gene Expression; D005956:Glucose-6-Phosphate Isomerase / Q000096:biosynthesis*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis*; D060285:Receptors, Autocrine Motility Factor; D018121:Receptors, Cytokine / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis; D015996:Survival Rate; D044767:Ubiquitin-Protein Ligases
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D018121:Receptors, Cytokine; C555000:AMFR protein, human; D060285:Receptors, Autocrine Motility Factor; D044767:Ubiquitin-Protein Ligases; D005956:Glucose-6-Phosphate Isomerase