The relationship between histone H3 phosphorylation and acetylation throughout the mammalian cell cycle.
During interphase, histone amino-terminal tails play important roles in regulating the extent of DNA compaction. Post-translational modifications of the histone tails are intimately associated with regulating chromatin structure: phosphorylation of histone H3 is associated with proper chromosome condensation and dynamics during mitosis, while multiple H2B, H3, and H4 tail acetylations destabilize the chromatin fiber and are sufficient to decondense chromatin fibers in vitro. In this study, we investigate the spatio-temporal dynamics of specific histone H3 phosphorylations and acetylations to better understand the interplay of these post-translational modifications throughout the cell cycle. Using a panel of antibodies that individually, or in combination, recognize phosphorylated serines 10 and 28 and acetylated lysines 9 and 14, we define a series of changes associated with histone H3 that occur as cells progress through the cell cycle. Our results establish that mitosis appears to be a period of the cell cycle when many modifications are highly dynamic. Furthermore, they suggest that the upstream histone acetyltransferases/deacetylases and kinase/phosphatases are temporally regulated to alter their function globally during specific cell cycle time points.
McManus
Kirk J
KJ
Department of Oncology, University of Alberta, Cross Cancer Institute, 11560 University Avenue, Edmonton, AB T6G 1Z2, Canada.
Hendzel
Michael J
MJ
D000107:Acetylation; D000818:Animals; D019556:COS Cells; D002453:Cell Cycle; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D002875:Chromosomes / Q000378:metabolism* / Q000648:ultrastructure; D000939:Epitopes / Q000502:physiology; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D018668:Muntjacs; D009707:Nucleosomes / Q000648:ultrastructure; D010766:Phosphorylation
D000939:Epitopes; D006657:Histones; D009707:Nucleosomes