title: The TCL1 oncoprotein binds the RNase PH domains of the PNPase exoribonuclease without affecting its RNA degrading activity.
abstract: TCL1 is an AKT kinase coactivator that, when dysregulated, initiates mature lymphocyte malignancies in humans and transgenic mice. While TCL1 augments AKT pathway signaling, additional TCL1 interacting proteins that may contribute to cellular homeostasis or transformation are lacking. Here, an exoribonuclease, PNPase, was identified in a complex with TCL1. The AKT interaction domain on TCL1 bound either RNase PH repeat domain of PNPase without influencing its RNA degrading activity, which was compatible with predicted docking models for a TCL1-PNPase complex. Our data provide a novel protein interaction for mammalian PNPase that may impact TCL1 mediated transformation.
authors.affiliation: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1732, USA.
authors.forename: Samuel W
authors.initials: SW
authors.lastname: French
authors.forename: David W
authors.initials: DW
authors.lastname: Dawson
authors.forename: Hsiao-Wen
authors.initials: HW
authors.lastname: Chen
authors.forename: Robert N
authors.initials: RN
authors.lastname: Rainey
authors.forename: Stuart A
authors.initials: SA
authors.lastname: Sievers
authors.forename: Cynthia E
authors.initials: CE
authors.lastname: Balatoni
authors.forename: Larry
authors.initials: L
authors.lastname: Wong
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Troke
authors.forename: Mai T N
authors.initials: MT
authors.lastname: Nguyen
authors.forename: Carla M
authors.initials: CM
authors.lastname: Koehler
authors.forename: Michael A
authors.initials: MA
authors.lastname: Teitell
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005095:Exoribonucleases / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D010957:Plasmids; D017434:Protein Structure, Tertiary; D011518:Proto-Oncogene Proteins / Q000737:chemistry* / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; C090851:TCL1A protein, human; D012313:RNA; D051057:Proto-Oncogene Proteins c-akt; D005095:Exoribonucleases