title: TRAIL-R4-beta: a new splice variant of TRAIL-receptor 4 lacking the cysteine rich domain 1.
abstract: Transcriptional modification by alternative splicing is known to be involved in the regulation of programmed cell death. Recently, alternative splice variants of the TNF-related apoptosis inducing ligand (TRAIL/APO2L) and of the death receptor TRAIL-R2/DR5 have been identified. In this study, we report the identification of a novel alternative splice variant of the decoy receptor with a truncated death domain TRAIL-R4 lacking exon 3, which we designated TRAIL-R4-beta. As revealed by BLAST search we identified the genomic organisation of the TRAIL-R4 gene which consists of 9 exons. Loss of exon 3 resulted in the truncation of the first complete cysteine rich domain 1 which is known to be involved in ligand-receptor-complex. In conclusion, alternative splicing might be involved in functional fine-tuning of TRAIL-induced programmed cell death.
authors.affiliation: Department of General and Visceral Surgery, Heinrich Heine University Duesseldorf, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Krieg
authors.forename: Jan
authors.initials: J
authors.lastname: Schulte am Esch
authors.forename: Uwe
authors.initials: U
authors.lastname: Ramp
authors.forename: Stefan B
authors.initials: SB
authors.lastname: Hosch
authors.forename: Wolfram T
authors.initials: WT
authors.lastname: Knoefel
authors.forename: Helmut E
authors.initials: HE
authors.lastname: Gabbert
authors.forename: Csaba
authors.initials: C
authors.lastname: Mahotka
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D045744:Cell Line, Tumor; D003545:Cysteine / Q000737:chemistry*; D005091:Exons; D006801:Humans; D007438:Introns; D008024:Ligands; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D053319:Tumor Necrosis Factor Decoy Receptors
chemical_list: D008024:Ligands; D020033:Protein Isoforms; D018124:Receptors, Tumor Necrosis Factor; C451501:TNFRSF10D protein, human; D053319:Tumor Necrosis Factor Decoy Receptors; D003545:Cysteine