Repair of chronic osteochondral defects in the rat. A bone marrow-stimulating procedure enhanced by cultured allogenic bone marrow mesenchymal stromal cells.
Bone marrow mesenchymal stromal cells were aspirated from immature male green fluorescent protein transgenic rats and cultured in a monolayer. Four weeks after the creation of the osteochondral defect, the rats were divided into three groups of 18: the control group, treated with an intra-articular injection of phosphate-buffered saline only; the drilling group, treated with an intra-articular injection of phosphate-buffered saline with a bone marrow-stimulating procedure; and the bone marrow mesenchymal stromal cells group, treated with an intra-articular injection of bone marrow mesenchymal stromal cells plus a bone marrow-stimulating procedure. The rats were then killed at 4, 8 and 12 weeks after treatment and examined. The histological scores were significantly better in the bone marrow mesenchymal stromal cells group than in the control and drilling groups at all time points (p < 0.05). The fluorescence of the green fluorescent protein-positive cells could be observed in specimens four weeks after treatment.
Nishimori
M
M
Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan. mako7119@yahoo.co.jp
Deie
M
M
Kanaya
A
A
Exham
H
H
Adachi
N
N
Ochi
M
M
D000818:Animals; D030801:Animals, Genetically Modified; D001854:Bone Marrow Cells / Q000166:cytology* / Q000473:pathology; D016026:Bone Marrow Transplantation / Q000379:methods; D002358:Cartilage, Articular / Q000293:injuries* / Q000473:pathology / Q000601:surgery; D002478:Cells, Cultured; D002908:Chronic Disease; D024043:Collagen Type II / Q000032:analysis; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000032:analysis; D006614:Hindlimb; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation / Q000379:methods*; D017154:Stromal Cells / Q000637:transplantation
D024043:Collagen Type II; D049452:Green Fluorescent Proteins