Intracellular penetration and activity of DX-619 in human polymorphonuclear leukocytes.
The intracellular penetration and activity of DX-619 in human polymorphonuclear leukocytes have been evaluated. DX-619 reached intracellular concentrations 10 times higher than the extracellular concentrations reached. Uptake was rapid, reversible, nonsaturable, and affected by environmental temperature, some metabolic inhibitors, and a soluble membrane activator. DX-619 showed intracellular activity against Staphylococcus aureus.
García
Isabel
I
Department of Microbiology, School of Medicine, University of Seville, Seville-41009, Spain. igarcial@us.es
Ballesta
Sofía
S
Murillo
Concepción
C
Perea
Evelio J
EJ
Pascual
Alvaro
A
D002478:Cells, Cultured; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D011759:Pyrrolidines / Q000097:blood* / Q000493:pharmacokinetics; D015363:Quinolones / Q000097:blood* / Q000493:pharmacokinetics; D013203:Staphylococcal Infections / Q000097:blood / Q000188:drug therapy / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects
C505440:DX 619; D011759:Pyrrolidines; D015363:Quinolones