Complex inhibition of tyrosinase by thiol-composed Cu2+ chelators: a clue for designing whitening agents.
The inhibition of tyrosinase has attracted considerable attention for potential medicinal and cosmetic applications, as well as in agriculture. This study investigated the inhibition effects of thiol-associated Cu(2+) chelators and deduced a strategy for designing and/or selecting tyrosinase inhibitors. Among the several compounds tested, dithioglycerine (DTGC) was selected for further experiments on the inhibition kinetics on tyrosinase. Different types of tyrosinases derived from mushroom and from the transient overexpression in HEK293 cells were tested individually. The results showed that DTGC significantly inhibited human tyrosinase in a complex manner (slope-parabolic mixed-type inhibition), which was comparable to mushroom tyrosinase. The affinity of DTGC affinity to human tyrosinase was evaluated by setting up a K(i slope) equation. The results suggest that a Cu(2+) chelator modified with thiol groups has potential as a whitening agent. In addition, a strategy for designing and/or selecting tyrosinase inhibitors that target the active enzyme site was also suggested.
Park
Yong-Doo
YD
Department of Dermatology, Sungkyunkwan University junmo.yang@samsung.com.
Lyou
You-Jeong
YJ
Hahn
Hwa-Sun
HS
Hahn
Myong-Joon
MJ
Yang
Jun-Mo
JM
D002478:Cells, Cultured; D002614:Chelating Agents / Q000737:chemistry / Q000494:pharmacology*; D003300:Copper / Q000378:metabolism*; D005990:Glycerol / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008956:Models, Chemical; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000494:pharmacology*
D002614:Chelating Agents; D013438:Sulfhydryl Compounds; D003300:Copper; D014442:Monophenol Monooxygenase; D005990:Glycerol