Biosurfactant production by Rhodococcus erythropolis grown on glycerol as sole carbon source.
The production of biosurfactant by Rhodococcus erythropolis during the growth on glycerol was investigated. The process was carried out at 28 degrees C in a 1.5-L bioreactor using glycerol as carbon source. The bioprocess was monitored through measurements of biosurfactant concentration and glycerol consumption. After 51 h of cultivation, 1.7 g/L of biosurfactant, surface, and interfacial tensions values (with n-hexadecane) of 43 and 15 mN/m, respectively, 67% of Emulsifying Index (E24), and 94% of oil removal were obtained. The use of glycerol rather than what happens with hydrophobic carbon source allowed the release of the biosurfactant, originally associated to the cell wall.
Ciapina
Elisa M P
EM
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, Rio de Janeiro, RJ, CEP 21949-900, Brasil.
Melo
Walber C
WC
Santa Anna
Lidia M M
LM
Santos
Alexandre S
AS
Freire
Denise M G
DM
Pereira Júnior
Nei
N
D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D005990:Glycerol / Q000378:metabolism*; D012240:Rhodococcus / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D013501:Surface-Active Agents / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*
D012722:Sewage; D013501:Surface-Active Agents; D002244:Carbon; D005990:Glycerol