title: [Expression of anti-HBc single-chain variable fragment mediated by recombinant replication defective adenovirus in vitro].
abstract: OBJECTIVE
  To define the expression of single-chain variable fragment (ScFv) against hepatitis B virus core protein (HBc) mediated by recombinant replication defective adenovirus carrying the anti-HBc ScFv gene in vitro and to define the activity of anti-HBc ScFv combining HBcAg.
  
  
  METHODS
  The recombinant adenoviruses carrying anti-HBc ScFv gene generated by homologous recombination in bacteria and packaged in 293 cells were transfected into HepG2 cells, and the anti-HBc ScFv was detected using SDS-PAGE and Western blot.
  
  
  RESULTS
  Green fluorescent protein (GFP) was observed in HepG2 cells after the transfection. SDS-PAGE displayed a protein strap about 2.7 x 10(4), and the result of Western blot displayed a positive reactive strap.
  
  
  CONCLUSIONS
  Anti-HBc ScFv can be expressed in cells mediated by recombinant replication defective adenovirus carrying the anti-HBc ScFv gene.
authors.affiliation: Department of Infectious Diseases, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, China. tzhhao@hotmail.com
authors.forename: Zheng-hao
authors.initials: ZH
authors.lastname: Tang
authors.forename: Yong-sheng
authors.initials: YS
authors.lastname: Yu
authors.forename: Hui-hui
authors.initials: HH
authors.lastname: Ma
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Min
authors.initials: M
authors.lastname: Xi
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: Zang
authors.forename: Ji-lu
authors.initials: JL
authors.lastname: Yao
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D002460:Cell Line; D006510:Hepatitis B Antibodies / Q000235:genetics* / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D057127:Single-Chain Antibodies / Q000235:genetics* / Q000276:immunology*; D014162:Transfection
chemical_list: D006510:Hepatitis B Antibodies; D057127:Single-Chain Antibodies