Comparison of carbonate apatite and beta-tricalcium phosphate (resorbable calcium phosphates) implanted subcutaneously into the back of rats.
Bioresorption and biocompatibility of carbonate apatites, both sintered and non-sintered (S-CAP and N-CAP), and of sintered beta-tricalcium phosphate (beta-TCP) were compared by implanting particles of these materials into the back of adult rats. Bioresorption--when evaluated non-destructively with non-decalcified tissues using microfocus X-ray tomography--was essentially the same for N-CAP and beta-TCP, while S-CAP exhibited statistically lower bioresorption at 2, 4, and 12 weeks postoperatively. Biocompatibility--when evaluated by ED1 immunostaining--was in the order of beta-TCP > N-CAP > S-CAP. The intensity of ED1 immunostaining decreased with time, but persisted longer in beta-TCP than in S-CAP and N-CAP, indicating that beta-TCP produced the strongest and most enduring stimulation of macrophages. Although no statistical differences were found in tartrate-resistant acid phosphatase (TRAP) staining among the materials at each implantation period, the degree of TRAP staining for S-CAP was statistically greater at 12 weeks than at 2 and 4 weeks, indicating that osteoclast-like cells were in part responsible for the resorption of the carbonate apatite.
Nagayama
Motohiko
M
Department of Oral Pathology, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry, Hozumi 1851, Mizuho, Gifu 501-0296, Japan.
Takeuchi
Hiroshi
H
Doi
Yutaka
Y
D020341:Absorbable Implants; D000704:Analysis of Variance; D000818:Animals; D001031:Apatites / Q000493:pharmacokinetics*; D001672:Biocompatible Materials / Q000493:pharmacokinetics*; D002130:Calcium Phosphates / Q000493:pharmacokinetics*; D008422:Materials Testing; D010010:Osteoclasts / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D001031:Apatites; D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; C030782:carboapatite