Capture of flowing human neutrophils by immobilised immunoglobulin: roles of Fc-receptors CD16 and CD32.
We investigated capture and activation of flowing human neutrophils through their Fc-receptors, FcRgammaIIIB (CD16) and FcRgammaIIA (CD32). Immobilised platelets bearing murine monoclonal antibody against glycoprotein IIbIIIA were able to capture and activate flowing neutrophils. The activation response was inhibited by antibody blockade of neutrophil CD32. However, capture only occurred efficiently at wall shear stress below 0.1 Pa if platelet P-selectin was blocked. If neutrophils were perfused over immobilised human IgG, many adhered at 0.025 or 0.05 Pa, but not at 0.1 Pa. Adhesion was reduced by blockade of CD16 or CD32, but blockade of CD16 had the greater effect. When neutrophils were perfused over a combination of purified P-selectin and IgG, blockade of CD16 and CD32 inhibited activation of captured cells. Immunoglobulin deposited in tissue could capture and activate slow-flowing neutrophils. It might also potentiate inflammatory responses at higher stress if presented along with selectins. The dominant FcR for capture of neutrophils was CD16, but with murine antibody, CD32 played a greater role.
Skilbeck
Christopher A
CA
Department of Physiology, The Medical School, The University of Birmingham, Birmingham B15 2TT, UK.
Lu
Xiaomei
X
Sheikh
Sajila
S
Savage
Caroline O S
CO
Nash
Gerard B
GB
D000818:Animals; D015703:Antigens, CD / Q000502:physiology*; D002465:Cell Movement / Q000276:immunology*; D058851:GPI-Linked Proteins; D006801:Humans; D007136:Immunoglobulins / Q000502:physiology*; D051379:Mice; D018375:Neutrophil Activation / Q000276:immunology; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D017452:Receptors, IgG / Q000502:physiology*
D015703:Antigens, CD; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D007136:Immunoglobulins; D017452:Receptors, IgG