title: [Analysis of anisodine and its metabolites in rat plasma by liquid chromatography-tandem mass spectrometry].
abstract: OBJECTIVE
  To identify anisodine and its metabolites in rat plasma after ingestion of anisodine by combining liquid chromatography and tandem mass spectrometry (LC-MS(n)).
  
  
  METHODS
  Plasma samples from rats after a single orally administration of 20 mg anisodine were added with methanol to precipitate protein. Then, it was analyzed by LC-MS(n). Identification and structural elucidation of the metabolites were performed by comparing their changes in molecular masses, retention-times and full scan MS(n) spectra with those of the parent drug and blank plasma.
  
  
  RESULTS
  The results revealed that the parent drug and its four metabolites (norscopine, scopine, hydroxyanisodine, N-oxide anisodine) existed in rat plasma.
  
  
  CONCLUSIONS
  This method is sensitive, rapid, simple, and it is suitable for the rapid identification of drug and its metabolits.
authors.affiliation: Hubei Province Key Lab of Bio-Technology of Traditional Chinese Medicine, Hubei University, Wuhan 430062, China.
authors.forename: Huai-Xia
authors.initials: HX
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Du
authors.forename: Feng-Mei
authors.initials: FM
authors.lastname: Han
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012602:Scopolamine Derivatives / Q000302:isolation & purification / Q000378:metabolism*; D012680:Sensitivity and Specificity; D019657:Solanaceae / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D012602:Scopolamine Derivatives; C012183:anisodine