title: [Complementarity-determining region 3 analysis of T cell receptor beta chain variable region in peripheral blood mononuclear cells of patients with systemic lupus erythematosus].
abstract: OBJECTIVE
  To analyze the drift of the complementarity-determining region 3 (CDR3) of T cell receptor (TCR) beta chain variable region (TCR BV) in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus.
  
  
  METHODS
  Immunoscope spectratyping techniques was used to analyze the distribution of TCRbeta chain CDR3 in 5 normal blood donors and the dominant CDR3 in the PBMCs in 5 SLE patients. Sequence analysis of the CDR3 region in monoclonal or oligoclonal T cells was performed.
  
  
  RESULTS
  The spectratypes of TCR BV gene CDR3 region showed Gaussian distribution in the 5 normal blood donors. The 5 SLE patients, however, displayed anomalous proliferation and oligoclonal expansion of the T cells was observed in different TCR BV families with different CDR3 sequences.
  
  
  CONCLUSIONS
  Noticeable drift of TCRbeta chain CDR3 can be seen in active SLE, indicating possible association of selective expression of TCR with immune pathogenesis in SLE. Determination of specific TCR CDR3 sequence provides a new means for studying the pathogenesis and personalized treatment of SLE.
authors.affiliation: Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China. LuoWei 421@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Xin-sheng
authors.initials: XS
authors.lastname: Yao
authors.forename: Hong-yun
authors.initials: HY
authors.lastname: Zou
authors.forename: Qian
authors.initials: Q
authors.lastname: Wen
authors.forename: Guang-ping
authors.initials: GP
authors.lastname: Ruan
authors.forename: Xiao-ning
authors.initials: XN
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D022801:Complementarity Determining Regions / Q000235:genetics*; D005260:Female; D019674:Genes, T-Cell Receptor beta / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D022801:Complementarity Determining Regions