[Isolation and characterization of a benzothiophene-desulfurizing bacterium].
A benzothiophene-desulfurizing strain was isolated and identified as Gordonia sp. C-6, which could degrade benzothiophene (BT) in a way analogous to the 4S pathway. It was found strain C-6 had the ability to grow in KT-BT medium whose sole source of sulfur was BT or its derivatives, whereas it couldn't grow well in a medium with DBT and its derivatives as the sole source of sulfur, the desulfurized product was identified as 2-(2'-hydroxyphenyl) ethan-1-al or its isomer benzofuran by GC-MS analysis. Desulfurization activity of strain C-6 was investigated. About 0.15mmol/L(50%) BT was desulfurized by strain C-6 after it was incubated in KT-BT medium at 30 degrees C for 48h, the residual BT has volatilized during aerobic fermentation. The quantitative detection of the benzothiophene-desulfurizing products was established with o-hydroxyphenylacetic acid as standard compound, the standard curve was fitted by Matlab.
Li
Guo-qiang
GQ
College of Life Sciences, Nankai University, Tianjin, China.
Lee
Kyongho
K
Ma
Ting
T
Li
Shan-shan
SS
Li
Hong
H
Liang
Feng-lai
FL
Liu
Ru-lin
RL
D033601:Gordonia Bacterium / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013455:Sulfur / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*
D012336:RNA, Ribosomal, 16S; D013876:Thiophenes; C088015:benzothiophene; D013455:Sulfur