title: Repeated fluorescence in situ hybridization by a microwave-enhanced protocol.
abstract: A novel re-hybridization protocol for pathology archive sections that uses microwave-assisted fluorescence in situ hybridization (FISH) is described. Stripping the probe from the pathology archive sections with HCl and re-hybridizing with the next probe by intermittent microwave irradiation generated clear signals without background noise. Repeated stripping and hybridization with numerous bacterial artificial chromosome (BAC)-derived probes would identify the profile of genome-wide changes in small lesions on sections.
authors.affiliation: First Department of Pathology, Hamamatsu University School of Medicine, Hamamatsu, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kitayama
authors.forename: Hisaki
authors.initials: H
authors.lastname: Igarashi
authors.forename: Taeko
authors.initials: T
authors.lastname: Kozu
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Nagura
authors.forename: Yohei
authors.initials: Y
authors.lastname: Ohashi
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sugimura
mesh_terms: D002869:Chromosome Aberrations; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008872:Microwaves; D016612:Paraffin Embedding