Deletions encompassing the manganese superoxide dismutase gene in the Drosophila melanogaster genome.
Two deletions, Df(2R)Sod2-11 and Df(2R)Sod2-332, are recovered that encompass the manganese superoxide dismutase (MnSOD) gene or a null mutant referred to as SOD2n283 in Drosophila. Molecular analysis has revealed that the Df(2R)Sod2-332 deletion completely uncovered both MnSOD and its adjacent gene, Arp53D, whereas Df(2R)Sod2-11 was missing the promoter region of MnSOD gene. As a consequence of reduced MnSOD expression, these deletion heterozygotes are now sensitive to oxidative stress. Complementation analysis with some recently recovered deletions in the 53C/D region has established that other essential loci exist in this interval, and second, that Arp53D function is not essential for the survival of the organism. These deletions will be instrumental in the recovery of missense substitutions in the MnSOD peptide and their influence on oxidative stress resistance.
Belton
Amy
A
Biology Department, Howard University, Washington DC 20059, USA.
Paul
Anirban
A
Duttaroy
Atanu
A
D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics; D017353:Gene Deletion; D049750:Genome, Insect; D006720:Homozygote; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
D000199:Actins; C088885:Arp53D protein, Drosophila; D029721:Drosophila Proteins; D013482:Superoxide Dismutase