The MGF expression of osteoblasts in response to mechanical overload.
Cell proliferation and mRNA expression of insulin-like growth factor (IGF-I) and "mechanogrowth factor" (MGF) were studied in osteoblasts in response to overload. Static and cyclic-stretching were used to apply superphysiological strains to cells. Overload was found to increase cell growth. IGF-I and its splicing variant, MGF, were measured using reverse transcriptase-polymerase chain reaction method and were found to be regulated differentially by mechanical signals at the mRNA level. Cyclic-stretching had a more significant effect on cell proliferation and mRNA expression levels of IGF-I and MGF, while unstrained cells did not express MGF at the mRNA level. These results demonstrated that gene expression is regulated by mechanical stimulation. MGF expression in osteoblasts in response to strain may be related to an autocrine mechanism.
Tang
Li-Ling
LL
Key Laboratory of Biomechanics and Tissue Engineering of Ministry of Education, Bio-engineering College, Chongqing University, Chongqing 40004, PR China. lilingtang@yahoo.com.cn
Xian
Cheng-Yu
CY
Wang
Yuan-Liang
YL
D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D010006:Osteoblasts / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013314:Stress, Mechanical
D012333:RNA, Messenger; C519382:mechano-growth factor, rat; D007334:Insulin-Like Growth Factor I