title: [Effects of Saponin B from Anemarrhena asphodeloides Bunge on tau hyperphosphorylation induced by beta-amyloid peptide (25-35) in rats].
abstract: OBJECTIVE
  To investigate the changes in the expressions of p53, DKK (the inhibitor of Wnt pathway) and phosphorylated tau in rat bilateral hippocampus after beta-amyloid peptide (beta-AP) (25-35) injection, and observe the effect of saponin B from Anemarrhena asphodeloides Bunge (SAaB) in this model.
  
  
  METHODS
  After bilateral injection of beta-AP (25-35) into the hippocampus of rats, RT-PCR was performed for observing the changes in p53 and DKK mRNA expressions and immunochemistry carried out to detect the changes in phosphorylated tau protein.
  
  
  RESULTS
  RT-PCR showed increased p53 and DKK mRNA expression and immunochemistry revealed increased phosphorylated tau-positive cells in rat hippocampus after beta-AP (25-35) injection, and administration of SAaB significantly ameliorated these changes.
  
  
  CONCLUSIONS
  SAaB can significantly ameliorate beta-AP-induced tau hyperphosphorylation by inhibiting increased p53 and DKK mRNA expressions in response to beta-AP injection.
authors.affiliation: Department of Neurology, Wuhan General Hospital of Guangzhou Command, Wuhan 430070, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhong
authors.forename: Jie
authors.initials: J
authors.lastname: Tan
authors.forename: Shi
authors.initials: S
authors.lastname: Ouyang
authors.forename: Jiang-ping
authors.initials: JP
authors.lastname: Xu
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D031403:Anemarrhena / Q000737:chemistry*; D000818:Animals; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012503:Saponins / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; C501126:DKK1 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D012503:Saponins; D016159:Tumor Suppressor Protein p53; C074636:amyloid beta-protein (25-35); C509415:p53 protein (325-355), human; D016875:tau Proteins