Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) gene of Arabidopsis causes weak rcd1 phenotype with compromised oxidative-stress responses.
rcd1 is a mutant of Arabidopsis thaliana that is more resistant to methyl viologen, but more sensitive to ozone than the wild type. rcd1-2 is caused by a single nucleotide substitution that results in a premature stop codon at Trp-332. The rcd1-2 mRNA level does not change significantly with the mutation. Since overexpression of rcd1-1 cDNA has been shown to bring about an rcd1-like phenotype, we created and examined the overexpression lines of RCD1 by the use of the cauliflower mosaic virus 35S promoter. The transgenic lines exhibited a weak rcd1-like phenotype, although no resistance to methyl viologen was observed. Further, they fully complemented the aberrant rcd1-2 phenotype. Subcellular localization of RCD1 was examined by transiently expressing green fluorescent protein (GFP) fused with RCD1 in onion epidermal cells. GFP signals are observed as aggregated foci in the inner nuclear matrix-like region.
Fujibe
Takahiro
T
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan.
Saji
Hikaru
H
Watahiki
Masaaki K
MK
Yamamoto
Kotaro T
KT
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000187:drug effects / Q000235:genetics*; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000235:genetics; D009154:Mutation; D009687:Nuclear Proteins / Q000187:drug effects / Q000235:genetics*; D018384:Oxidative Stress / Q000502:physiology*; D010126:Ozone / Q000494:pharmacology; D010641:Phenotype; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012639:Seeds / Q000187:drug effects / Q000235:genetics
D029681:Arabidopsis Proteins; D009687:Nuclear Proteins; C492758:RCD1 protein, Arabidopsis; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D010126:Ozone