title: SSLP-1, a secreted Ly-6 protein purified from mouse seminal vesicle fluid.
abstract: The Ly-6 protein family refers to a group of glycophosphatidyl inositol-anchored membrane proteins with ten conserved cysteines. They are thought to be involved in cellular adhesion and signaling. Recently, a subfamily of secreted Ly-6 proteins has been identified. In the present study, we report a secreted Ly-6 protein, secreted seminal vesicle Ly-6 protein 1 (SSLP-1) purified from mouse seminal vesicles using a series of steps including ion-exchange chromatography on a diethylaminoethyl (DEAE)-Sephacel column, gel filtration on a Sephadex G-75 column, and ion-exchange HPLC on a sulfopropyl column. Further analysis demonstrated it to be a novel, previously unnamed, 17 kDa glycoprotein. N-glycosidase F treatment revealed a core protein with a molecular mass of 8720 Da. By Basic Local Alignment Search Tool Protein analysis, we found that SSLP-1 had ten conserved cysteine residues identical with other secreted Ly-6 proteins. The gene Gm191, which is located on chromosome 9, encodes SSLP-1. By Northern blotting with 21 different mouse tissues, we found that Sslp-1 mRNA was predominantly expressed in the seminal vesicle. Immunohistochemistry revealed SSLP-1 protein in the luminal fluid and mucosal epithelium of the seminal vesicles. The amount of Sslp-1 mRNA and SSLP-1 protein in the seminal vesicle was regulated by testosterone and correlated with the stage of animal maturation. The tissue-specific expression pattern suggests that SSLP-1 may play a physiological role in male mouse reproduction.
authors.affiliation: Department of Medical Research, Mackay Memorial Hospital, Tamshui 251, Taiwan, ROC. lsh@ms1.mmh.org.tw
authors.forename: Sheng-Hsiang
authors.initials: SH
authors.lastname: Li
authors.forename: Robert Kuo-Kuang
authors.initials: RK
authors.lastname: Lee
authors.forename: Ming-Huei
authors.initials: MH
authors.lastname: Lin
authors.forename: Yuh-Ming
authors.initials: YM
authors.lastname: Hwu
authors.forename: Chung-Hao
authors.initials: CH
authors.lastname: Lu
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Chen
authors.forename: Hsuan-Chiang
authors.initials: HC
authors.lastname: Chen
authors.forename: Wen-Hwei
authors.initials: WH
authors.lastname: Chang
authors.forename: Wei-Chao
authors.initials: WC
authors.lastname: Chang
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000032:analysis* / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D015870:Gene Expression / Q000187:drug effects; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D012661:Semen / Q000737:chemistry*; D012669:Seminal Vesicles / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment; D013739:Testosterone / Q000494:pharmacology
chemical_list: D000950:Antigens, Ly; D012333:RNA, Messenger; C520154:SSLP-1 protein, mouse; D013739:Testosterone