[Differentially express genes in human embryonic lung fibroblasts with damage tolerance induced by low-dose hydroquinone].
OBJECTIVE
To observe the differentially expressed genes in human embryonic lung fibroblasts (HELF) induced by small-dose hydroquinone (HQ) using fluorescence differential display-PCR (DD-PCR).


METHODS
According to the dose-effect relation of HQ toxicity we established previously, HQ dose that did not induce observed cell damage or proliferation arrest was defined as low dose (100 pmol/L), and that causing obvious cell damage as the high dose (100 micrommol/L). The cells were then treated with low or high dose of HQ, or exposed to high-dose HQ following pretreatment with low-dose HQ for some time, respectively. Fluorescence DD-PCR was performed and 33 differentially expressed genes were identified in the cells with different treatments, and 8 of the identified genes were amplified, cloned, sequenced and blasted.


RESULTS
Seven of the 8 amplified genes were unknown genes, and the left one was identified as a known gene highly homologous to that encoding Homo sapiens Rap1 interacting factor 1 (RIF1).


CONCLUSIONS
Low-dose HQ can induce damage tolerance in HELF, and identification of the differentially expressed genes may provide valuable sight into the mechanism of HQ-induced damage tolerance.
Wei
Qin-zhi
QZ
Department of Health Toxicology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. cnwei99@163.com
Zhuang
Zhi-xiong
ZX
D000222:Adaptation, Physiological / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006801:Humans; D006873:Hydroquinones / Q000494:pharmacology*; D008168:Lung / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods
D006873:Hydroquinones; C031927:hydroquinone