title: Molecular markers with potential to replace phage typing for Salmonella enterica serovar typhimurium.
abstract: Using Amplified Fragment Length Polymorphism (AFLP) analysis of isolates from 23 phage types, we isolated 11 molecular markers that are potentially useful for molecular typing of Salmonella enterica serovar typhimurium. We tested these and 11 previously studied markers for their ability to discriminate among isolates and for correlation of their distribution with phage types. The Simpson's index of discriminatory power for the molecular markers is 0.96. One hundred and twenty one isolates from 33 phage types tested were divided into 51 types which are further grouped into 24 patterns. Eight patterns can unambiguously identify 8 phage types and a further 12 correlated with phage type distribution, showing the usefulness of these markers for molecular phage typing.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia. r.lan@unsw.edu.au
authors.forename: Ruiting
authors.initials: R
authors.lastname: Lan
authors.forename: Gordon
authors.initials: G
authors.lastname: Stevenson
authors.forename: Kathy
authors.initials: K
authors.lastname: Donohoe
authors.forename: Linda
authors.initials: L
authors.lastname: Ward
authors.forename: Peter R
authors.initials: PR
authors.lastname: Reeves
mesh_terms: D001434:Bacteriophage Typing; D001483:Base Sequence; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012480:Salmonella Infections / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial