Fine-needle aspiration biopsy of non-Hodgkin lymphoma for use in expression microarray analysis.
BACKGROUND
The majority of patients with lymphoma undergo a single biopsy for diagnosis, and there are few opportunities to acquire posttreatment material. Fine-needle aspiration (FNA) biopsy is a minimally invasive procedure, and acquiring routine posttreatment material would require minimal effort and provide needed material for gene expression profiling.


METHODS
Ex vivo FNA biopsies were performed using a standard clinical technique with 21-gauge, 22-gauge, 23-gauge, and 25-gauge needles for 8 lymph node specimens and were collected in either RNA stabilization reagent (RNAlater) or Trizol. Eight patients with known or suspected Non-Hodgkin lymphoma (NHL) underwent interoperative (in vivo) FNA biopsies based on the best technique derived from the ex vivo aspirates. RNA derived from the in vivo FNA biopsies and the matched, snap-frozen surgical lymph node biopsy remnant was used for gene expression analysis with proprietary U133A chips.


RESULTS
The results confirmed the authors' experience, that RNA isolated from FNA biopsies of lymph nodes collected in Trizol is superior both quantitatively and qualitatively to RNA collected in RNAlater. Gene expression profiles of NHL derived from in vivo FNA biopsies and matched, frozen surgical specimens showed good overall correlation.


CONCLUSIONS
High-throughput gene expression analysis in patients with NHL derived from material acquired by FNA biopsy is a feasible approach to developing a platform for real-time analysis of treatment responses in this group of patients.
Morrison
Carl
C
Department of Pathology, The Arthur James Cancer Hospital and Richard Solove Research Institute, The Ohio State University Medical School, Columbus, Ohio, USA. carl.morrison@osumc.edu
Palatini
Jeff
J
Riggenbach
Judy
J
Radmacher
Michael
M
Porcu
Pierluigi
P
D044963:Biopsy, Fine-Needle; D020869:Gene Expression Profiling; D006801:Humans; D008198:Lymph Nodes / Q000601:surgery; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000601:surgery*; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000302:isolation & purification; D015203:Reproducibility of Results; D016707:Tissue Fixation / Q000379:methods
D012313:RNA