title: The power of cooperative investigation: summary and comparison of the HUPO Brain Proteome Project pilot study results.
abstract: Within the pilot phase of the HUPO Brain Proteome Project, nine participating laboratories analysed human (epilepsy and/or post mortem material) and mouse brain samples (embryonic, juvenile and adult), respectively, using a variety of different state of the art techniques. Thirty-seven different analytical approaches were accomplished. Of these analyses, 17 were done differentially, i.e. the protein expression patterns of the different samples (human or mouse) were compared. A catalogue of all proteins present in the respective sample was built in 20 analyses (mapping). All data were collected in the Data Collection Center in Bochum, Germany, and were reprocessed according to thoroughly defined parameters. In this report, a summary of all results and inter-laboratory comparisons with respect to the number of identified proteins, the analysed organism, and the used techniques is presented.
authors.affiliation: Medizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany.
authors.forename: Kai A
authors.initials: KA
authors.lastname: Reidegeld
authors.forename: Michael
authors.initials: M
authors.lastname: Müller
authors.forename: Christian
authors.initials: C
authors.lastname: Stephan
authors.forename: Martin
authors.initials: M
authors.lastname: Blüggel
authors.forename: Michael
authors.initials: M
authors.lastname: Hamacher
authors.forename: Lennart
authors.initials: L
authors.lastname: Martens
authors.forename: Gerhard
authors.initials: G
authors.lastname: Körting
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Chamrad
authors.forename: David
authors.initials: D
authors.lastname: Parkinson
authors.forename: Rolf
authors.initials: R
authors.lastname: Apweiler
authors.forename: Helmut E
authors.initials: HE
authors.lastname: Meyer
authors.forename: Katrin
authors.initials: K
authors.lastname: Marcus
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D006801:Humans; D051379:Mice; D015337:Multicenter Studies as Topic; D010865:Pilot Projects; D020543:Proteome / Q000032:analysis* / Q000378:metabolism
chemical_list: D020543:Proteome