title: [Evaluation of locomotor activity after a local induction of toxic demyelination in the brainstem of Wistar rats].
abstract: Ethidium-bromide (EB)-induced lesions have been used to investigate the incomplete remyelination in the central nervous system, as well as to evaluate therapeutic strategies to accelerate the reconstruction of the lost myelin sheaths. Although many electrophysiologic studies were performed in situations of experimental demyelination and remyelination, their behavioural effects have not been properly analyzed. In this study, we investigated ultrastructurally the EB - demyelinating lesions as well as the locomotor activity of rats during the beam walking test after a focal induction of demyelination using the EB model in the ventral surface of the brainstem. It was observed the occurrence of locomotor deficits until 31 days post-injection, as well as that subsequent remyelination was related to the return of the lost function.
authors.affiliation: Universidade Paulista, Rua Caconde 125/51, 01425-011 São Paulo SP, Brazil. bondan@uol.com.br
authors.forename: Eduardo Fernandes
authors.initials: EF
authors.lastname: Bondan
authors.forename: Maria Anete
authors.initials: MA
authors.lastname: Lallo
authors.forename: Heloísa
authors.initials: H
authors.lastname: Orsini
authors.forename: Henri Levi Donnaruma
authors.initials: HL
authors.lastname: Bentubo
authors.forename: Angela
authors.initials: A
authors.lastname: Yazbek
authors.forename: Daclê Juliani
authors.initials: DJ
authors.lastname: Macrini
authors.forename: Maria Martha
authors.initials: MM
authors.lastname: Bernardi
authors.forename: Dominguita Luhers
authors.initials: DL
authors.lastname: Graça
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D003711:Demyelinating Diseases / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D004996:Ethidium; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D009186:Myelin Sheath / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D004996:Ethidium