Modeling the influence of electron beam irradiation on the heat resistance of Bacillus cereus spores.
The effect of electron beam irradiation (EBI) on Bacillus cereus spore heat resistance was investigated. Irradiation with accelerated electrons had an important heat-sensitizing effect on distilled-water spore suspensions. After irradiation doses of 1.3, 3.1, or 5.7 kGy followed by heating at 90 degrees C, calculated D(90)-values for strains Escuela Politécnica Superior de Orihuela (EPSO)-41WR and EPSO-50UR were reduced more than 1.3, 2.4, and 4.6 times, respectively. Plots of calculated log D(T)-values versus irradiation doses (1.3, 3.1, and 5.7 kGy) yielded straight parallel lines for the 85-100 degrees C heating temperature range, which made it possible to develop an equation to predict the changes in heat sensitivity of B. cereus spores that occurred with changing irradiation dose. Radiation-induced heat-sensitivity was characterized by a z(EBI)-value which was determined as the irradiation dose that should be required to reduce the decimal reduction time (D(T)) by one log(10) cycle when log(10)D(T) was plotted against irradiation treatment. A model is proposed to describe the influence of a pre-irradiation treatment with electron beams followed by heating on the heat resistance of B. cereus spores. This study also suggests the potential use of EBI followed by heating for food preservation.
Valero
M
M
Departamento de Producción Vegetal y Microbiología, Escuela Po1itécnica Superior de Orihuela, Universidad Miguel Hernández - Campus de Orihuela, Carretera de Benie1, km 3.2, 03312-Orihuela, Alicante, Spain. m.valero@umh.es
Sarrías
J A
JA
Alvarez
D
D
Salmerón
M C
MC
D001409:Bacillus cereus / Q000254:growth & development / Q000502:physiology* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005514:Food Irradiation; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D005720:Gamma Rays; D006358:Hot Temperature; D013171:Spores, Bacterial / Q000254:growth & development; D013997:Time Factors