A rapid method for protein extraction from fission yeast.
Researchers working with fission yeast conduct protein extraction widely and frequently, but this includes the handling of glass beads, and hence is laborious and cumbersome, especially when dealing with a large number of samples. Here we describe a rapid and reliable method for preparing protein extract from fission yeast, one which is applicable to routine western blotting.
Matsuo
Yuzy
Y
Analytical Research Center for Experimental Sciences, Saga University, Japan.
Asakawa
Kazuhide
K
Toda
Takashi
T
Katayama
Satoshi
S
D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D012568:Schizosaccharomyces / Q000737:chemistry* / Q000166:cytology; D012972:Sodium Hydroxide / Q000737:chemistry*; D013997:Time Factors
D011506:Proteins; D012972:Sodium Hydroxide