Quantitative investigation of the interaction between granulocyte-macrophage colony-stimulating factor and heparin by capillary zone electrophoresis.
The interactions between granulocyte-macrophage colony-stimulating factor (GM-CSF) and heparin or low-molecular weight heparin (LMWH) were studied by CZE. It was found that GM-CSF could bind to both heparin and LMWH. The binding constants were calculated from Scatchard regression to be (6.5 +/- 0.8) x 10(5)/M and (11.2 +/- 0.7) x 10(5)/M, respectively. The specificity of the interaction between GM-CSF and heparin was also studied by employing another sulfated K carrageenan oligosaccharide as a control. Results showed that K carrageenan oligosaccharide could not interact with GM-CSF, indicating that GM-CSF could specifically interact with heparin.
Liang
Aiye
A
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Du
Yuguang
Y
Wang
Keyi
K
Lin
Bingcheng
B
D002351:Carrageenan / Q000737:chemistry / Q000378:metabolism; D004347:Drug Interactions; D019075:Electrophoresis, Capillary / Q000379:methods*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry* / Q000378:metabolism; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry / Q000378:metabolism; D066298:In Vitro Techniques; D011485:Protein Binding; D011994:Recombinant Proteins
D006495:Heparin, Low-Molecular-Weight; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D002351:Carrageenan; D006493:Heparin