Molecular markers with potential to replace phage typing for Salmonella enterica serovar typhimurium.
Using Amplified Fragment Length Polymorphism (AFLP) analysis of isolates from 23 phage types, we isolated 11 molecular markers that are potentially useful for molecular typing of Salmonella enterica serovar typhimurium. We tested these and 11 previously studied markers for their ability to discriminate among isolates and for correlation of their distribution with phage types. The Simpson's index of discriminatory power for the molecular markers is 0.96. One hundred and twenty one isolates from 33 phage types tested were divided into 51 types which are further grouped into 24 patterns. Eight patterns can unambiguously identify 8 phage types and a further 12 correlated with phage type distribution, showing the usefulness of these markers for molecular phage typing.
Lan
Ruiting
R
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia. r.lan@unsw.edu.au
Stevenson
Gordon
G
Donohoe
Kathy
K
Ward
Linda
L
Reeves
Peter R
PR
D001434:Bacteriophage Typing; D001483:Base Sequence; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012480:Salmonella Infections / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial