The regulatory role of Hyper-IL-6 in the differentiation of myeloid and erythroid progenitors derived from human cord blood.
This study was designed to investigate the regulatory role of soluble interleukin-6 receptor (sIL-6R) and interleukin-6 (IL-6) fusion protein (Hyper-IL-6) in the differentiation of human myeloid and erythroid progenitors by a serum-free liquid suspension culture system, using the human cord blood-derived CD34(+)CD38(-) cells as a target. We found that Hyper-IL-6 promoted the generation of CD15(+) granulocytic and CD14(+) monocytic cells and suppressed that of CD14(-)CD1a(+) dendritic cells from CD36(-)CD15(-)CD14(-)CD1a(-)IL-6R(+) myeloid progenitors. Conversely, CD34(+)CD38(-) cell-derived early erythroid progenitors were negative for IL-6R expression. Hyper-IL-6 potentiated the generation of CD36(+)glycophorinA(high) mature erythroid cells from the IL-6R(-) early erythroid progenitors. Our results indicate that Hyper-IL-6 augments the generation of CD15(+) granulocytic, CD14(+) monocytic and CD36(+)glycophorinA(high) cell and suppresses that of CD14(-)CD1a(+) dendritic cells.
Yu
Zhaocai
Z
Department of Clinical Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. yzhaocai@fmmu.edu.cn <yzhaocai@fmmu.edu.cn>
Liu
Wenchao
W
Liu
Duhu
D
Fan
Li
L
D000276:Adjuvants, Immunologic / Q000235:genetics / Q000502:physiology*; D018949:Antigens, CD1 / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology*; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000276:immunology; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D006131:Growth Inhibitors / Q000235:genetics / Q000502:physiology; D006410:Hematopoiesis / Q000235:genetics / Q000276:immunology; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000502:physiology*; D016256:Lewis X Antigen / Q000096:biosynthesis; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis / Q000378:metabolism; D023461:Myeloid Progenitor Cells / Q000166:cytology* / Q000276:immunology; D019947:Receptors, Interleukin-6 / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000502:physiology*
D000276:Adjuvants, Immunologic; D018949:Antigens, CD1; C419393:CD1a antigen; D006131:Growth Inhibitors; C508600:IL6 protein, human; D015850:Interleukin-6; D016256:Lewis X Antigen; D018950:Lipopolysaccharide Receptors; D019947:Receptors, Interleukin-6; D011993:Recombinant Fusion Proteins