title: Construction of comparative genetic maps of two 4Bs.4Bl-5Rl translocations in bread wheat (Triticum aestivum L.).
abstract: The physical length of the rye segment of a 4BS.4BL-5RL translocation derived from the Cornell Wheat Selection 82a1-2-4-7 in a Triticum aestivum 'Chinese Spring' background was measured using genomic in situ hybridization (GISH) and found to be 16% of the long arm. The size of this translocation was similar to previously published GISH measurements of another 4BS.4BL-5RL translocation in a Triticum aestivum 'Viking' wheat background. Molecular maps of both 4BS.4BL-5RL translocations for 2 different wheat backgrounds were developed using RFLP analysis. The locations of the translocation breakpoints of the 2 4BS.4BL-5RL translocations were similar even though they arose in different populations. This suggests a unique property of the region at or near the translocation breakpoint that could be associated with their similarity and spontaneous formation. These segments of rye chromosome 5 also contain a gene for copper efficiency that improves the wheat's ability to cope with low-copper soils. Genetic markers in these maps can also be used to screen for copper efficiency in bread wheat lines derived from the Cornell Wheat Selection 82a1 2-4-7.
authors.affiliation: Plant and Food Science, School of Agriculture, Food and Wine, The University of Adelaide, Australia. richard.leach@adelaide.edu.au
authors.forename: R C
authors.initials: RC
authors.lastname: Leach
authors.forename: I S
authors.initials: IS
authors.lastname: Dundas
authors.forename: A
authors.initials: A
authors.lastname: Houben
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018745:Genome, Plant; D017403:In Situ Hybridization; D011110:Polymorphism, Genetic; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*