Spectrophotometric determination of certain antidepressants in pharmaceutical preparations.
Simple and reproducible spectrophotometric methods have been developed for determination of sertraline, fluoxetine, and venlafaxine in pharmaceutical preparations. The methods are based on the reactions between the studied drug substances and ion-pair agents (bromothymol blue, bromocresol green, or bromophenol blue) to produce yellow-colored ion-pair complexes in acidic buffers. After extracting in chloroform, the ion-pair complexes are spectrophotometrically determined at the optimum wavelength. Optimizations of the reaction conditions were carried out. Beer's law was obeyed within the concentration range from 1 to 15 microg/mL. The molar absorptivity, Sandell sensitivity, and detection and quantification limits were also determined. The developed methods were applied successfully for the determination of these drugs in some available commercial preparations. The results were compared statistically with those obtained from reported high-performance liquid chromatography methods.
Onal
Armağan
A
Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry, 34116, Beyazit, Istanbul, Turkey. armaganozkul@yahoo.com
Kepekci
S Evrim
SE
Cetin
S Müge
SM
Ertürk
Sidika
S
D000928:Antidepressive Agents / Q000032:analysis* / Q000494:pharmacology*; D001961:Bromcresol Green / Q000032:analysis; D001978:Bromphenol Blue / Q000032:analysis; D001979:Bromthymol Blue / Q000032:analysis; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D003511:Cyclohexanols / Q000032:analysis*; D005473:Fluoxetine / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020280:Sertraline / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods*; D000069470:Venlafaxine Hydrochloride
D000928:Antidepressive Agents; D003511:Cyclohexanols; D005473:Fluoxetine; D001978:Bromphenol Blue; D000069470:Venlafaxine Hydrochloride; D001961:Bromcresol Green; D020280:Sertraline; D001979:Bromthymol Blue