title: Probing the adaptive response of Escherichia coli to extracellular Zn(II).
abstract: The adaptive response of Escherichia coli cells to differing intracellular and extracellular Zn(II) concentrations was evaluated by two-dimensional gel electrophoresis and peptide identifications. Twenty-one Zn(II)-responsive proteins, which were previously not known to be associated with Zn(II), were identified. Most of the proteins were related to cellular metabolism and include membrane transporters and glycolytic and TCA-associated enzymes. The expression levels of no known Zn(II) transporters were identified with these studies. The results of these studies suggest a role of Zn(II) in the expression levels of several E. coli proteins, and the results are discussed in light of recent genomic profiling studies on the adaptive response of E. coli cells to stress by Zn(II) excess.
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, 112 Hughes Hall, Oxford, OH 45056, USA.
authors.forename: Tara K
authors.initials: TK
authors.lastname: Sigdel
authors.forename: Renee
authors.initials: R
authors.lastname: Cilliers
authors.forename: Priya R
authors.initials: PR
authors.lastname: Gursahaney
authors.forename: Peter
authors.initials: P
authors.lastname: Thompson
authors.forename: J Allen
authors.initials: JA
authors.lastname: Easton
authors.forename: Michael W
authors.initials: MW
authors.lastname: Crowder
mesh_terms: D048708:Cell Growth Processes; D048430:Cell Shape; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D010455:Peptides; D015032:Zinc