title: Effects of genetic polymorphisms CYP1A1, GSTM1, GSTT1 and GSTP1 on urinary 1-hydroxypyrene levels in sugarcane workers.
abstract: Sugarcane workers in Brazil are exposed to various genotoxic compounds, including polycyclic aromatic hydrocarbons (PAHs), derived from an incomplete combustion process of burnt sugarcane fields. The effects of the occupational exposure to sugarcane fields burning were measured in urine samples of sugarcane workers from the northwest of the State of São Paulo when exposed (harvesting) and when non-exposed (non-harvesting). The urinary levels of 1-hydroxypyrene (1-OHP) and the influence of the genetic polymorphisms CYP1A1, GSTM1, GSTT1 and GSTP1 were evaluated. Our results showed that the 1-OHP levels were significantly higher (P<0.0000) in the exposed sugarcane workers (0.318 mumol mol(-1) creatinine) than in the non-exposed workers (0.035 mumol mol(-1) creatinine). In an unvaried analysis, no influence regarding the polymorphisms was observed. However, multivariate regression analysis showed that the CYP1A1()4 polymorphism in the exposed group, and age and the GSTP1 polymorphism in the non-exposed group significantly influenced urinary 1-OHP excretion levels (P<0.10). The same group of sugarcane workers was significantly more exposed to PAHs during the harvesting period than during the non-harvesting period.
authors.affiliation: Biology Department, UNESP, São Paulo State University, São José do Rio Preto, SP, Brazil.
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: do Vale Bosso
authors.forename: Lidia Maria Fonte
authors.initials: LM
authors.lastname: Amorim
authors.forename: Sandro José
authors.initials: SJ
authors.lastname: Andrade
authors.forename: Ana
authors.initials: A
authors.lastname: Rossini
authors.forename: Mary Rosa Rodrigues
authors.initials: MR
authors.lastname: de Marchi
authors.forename: Antonio Ponce
authors.initials: AP
authors.lastname: de Leon
authors.forename: Claudia Marcia Aparecida
authors.initials: CM
authors.lastname: Carareto
authors.forename: Nívea Dulce Tedeschi
authors.initials: ND
authors.lastname: Conforti-Froes
mesh_terms: D000328:Adult; D000368:Aged; D000383:Agriculture; D015415:Biomarkers / Q000652:urine; D001938:Brazil; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D004784:Environmental Monitoring; D005260:Female; D051549:Glutathione S-Transferase pi / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D011110:Polymorphism, Genetic; D011721:Pyrenes / Q000378:metabolism*; D031786:Saccharum; D016482:Urinalysis
chemical_list: D015415:Biomarkers; D019363:Cytochrome P-450 CYP1A1; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D011721:Pyrenes; C413545:glutathione S-transferase T1; C033146:1-hydroxypyrene