Altered hippocampal synaptic potentiation in P2X4 knock-out mice.
P2X4 purinergic receptors are calcium-permeable, ATP-activated ion channels. In the CA1 area of the hippocampus, they are located at the subsynaptic membrane somewhat peripherally to AMPA receptors. The possible role of P2X4 receptors has been difficult to elucidate because of the lack of selective antagonists. Here we report the generation of a P2X4 receptor knock-out mouse and show that long-term potentiation (LTP) at Schaffer collateral synapses is reduced relative to that in wild-type mice. Ivermectin, which selectively potentiates currents at P2X4, was found to increase LTP in wild-type mice but had no effect in P2X4 knock-out mice. We suggest that calcium entry through subsynaptic P2X4 receptors during high-frequency stimulation contributes to synaptic strengthening.
Sim
Joan A
JA
Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom.
Chaumont
Séverine
S
Jo
Jihoon
J
Ulmann
Lauriane
L
Young
Mark T
MT
Cho
Kwangwook
K
Buell
Gary
G
North
R Alan
RA
Rassendren
Francois
F
D000818:Animals; D006624:Hippocampus / Q000502:physiology*; D007150:Immunohistochemistry; D007559:Ivermectin / Q000494:pharmacology; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D018048:Receptors, Purinergic P2 / Q000172:deficiency / Q000187:drug effects / Q000502:physiology*; D058848:Receptors, Purinergic P2X4; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects
C548227:P2rx4 protein, mouse; D018048:Receptors, Purinergic P2; D058848:Receptors, Purinergic P2X4; D007559:Ivermectin