Jasmonic acid signalling and herbivore resistance traits constrain regrowth after herbivore attack in Nicotiana attenuata.
Because traits conferring resistance on herbivores can reduce fitness-associated traits, trade-offs may occur between tolerance and resistance responses. We examined these trade-offs in genotypes of Nicotiana attenuata that were transformed to silence trypsin proteinase inhibitor (TPI) production (AS-Natpi), an antiherbivore defense associated with (14%) reductions in seed production, and the jasmonate signal cascade that elicits these defenses (AS-Nalox3), by measuring stalk and axillary branch growth and seed production after two defoliation regimes and Manduca sexta larval attack to bottom or middle and top stalk leaves. Larval attack and defoliation at middle and top leaves depressed seed production and increased axillary branching more than at bottom leaves. AS-Nalox3 and AS-Natpi plants produced significantly longer (two- to fourfold) branches than did wild-type (WT) plants, results that are consistent with resource-based trade-offs between resistance and regrowth. Methyl jasmonate (MeJA) treatment of AS-Nalox3 plants restored WT branch growth, suggesting that jasmonic acid (JA) signalling suppresses regrowth and contributes to apical dominance. These results are consistent with the existence of JA- and resource-mediated trade-offs between regrowth and herbivore resistance traits.
Zavala
Jorge A
JA
Max Planck Institute for Chemical Ecology, Department of Molecular Ecology, Hans-Knöll-Str. 8, Beutenberg Campus, 07745 Jena, Germany.
Baldwin
Ian T
IT
D000085:Acetates / Q000378:metabolism* / Q000494:pharmacology*; D000818:Animals; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology*; D005247:Feeding Behavior / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D007814:Larva / Q000502:physiology; D018402:Manduca / Q000502:physiology*; D054883:Oxylipins; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D014026:Nicotiana / Q000378:metabolism*
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C072239:methyl jasmonate