A protocol for combined delipidation and subfractionation of membrane proteins using organic solvents.
Schröder
Bernd
B
Institute of Physiological Chemistry, Philipps-University Marburg, 35033 Marburg, Germany. bernd.schroeder@staff.uni-marburg.de
Hasilik
Andrej
A
D002725:Chloroform / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008055:Lipids / Q000737:chemistry* / Q000302:isolation & purification*; D008247:Lysosomes / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification*; D000432:Methanol / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D014269:Trifluoroacetic Acid / Q000737:chemistry
D008055:Lipids; D008565:Membrane Proteins; D012997:Solvents; D002725:Chloroform; D014269:Trifluoroacetic Acid; D000432:Methanol