title: Comparative cDNA-AFLP analysis of Cd-tolerant and -sensitive genotypes derived from crosses between the Cd hyperaccumulator Arabidopsis halleri and Arabidopsis lyrata ssp. petraea.
abstract: Cadmium (Cd) tolerance seems to be a constitutive species-level trait in Arabidopsis halleri. In order to identify genes potentially implicated in Cd tolerance, a backcross (BC1) segregating population was produced from crosses between A. halleri ssp. halleri and its closest non-tolerant relative A. lyrata ssp. petraea. The most sensitive and tolerant genotypes of the BC1 were analysed on a transcriptome-wide scale by cDNA-amplified fragment length polymorphism (AFLP). A hundred and thirty-four genes expressed more in the root of tolerant genotypes than in sensitive genotypes were identified. Most of the identified genes showed no regulation in their expression when exposed to Cd in a hydroponic culture medium and belonged to diverse functional classes, including reactive oxygen species (ROS) detoxification, cellular repair, metal sequestration, water transport, signal transduction, transcription regulation, and protein degradation, which are discussed.
authors.affiliation: Laboratoire de Physiologie et Génétique Moléculaire des Plantes, Université Libre de Bruxelles, Campus Plaine CP242, B-1050 Brussels, Belgium.
authors.forename: Adrian Radu
authors.initials: AR
authors.lastname: Craciun
authors.forename: Mikael
authors.initials: M
authors.lastname: Courbot
authors.forename: Fabienne
authors.initials: F
authors.lastname: Bourgis
authors.forename: Pietrino
authors.initials: P
authors.lastname: Salis
authors.forename: Pierre
authors.initials: P
authors.lastname: Saumitou-Laprade
authors.forename: Nathalie
authors.initials: N
authors.lastname: Verbruggen
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism* / Q000494:pharmacology; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000737:chemistry; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005838:Genotype; D018527:Hydroponics; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D018076:DNA, Complementary; D002104:Cadmium