[Immunization with cellulose-immobilized antigens. The development of A. E. Gurvitch concept].
A highly purified TUL4-CBD chimeric protein was obtained by one stage purification method. TUL4-CBD protein consists of TUL4 Francisella tularensis mature peptide sequence, Gly-Ser spacer and cellulose binding domain (CBD) of Anaerocellum thermophilum. The TUL4-CBD protein was shown to induce production of specific antibodies to TUL4 protein in laboratory animals.
Liashchuk
A M
AM
Lunin
V G
VG
Kariagina
A S
AS
Lavrova
N V
NV
Rodionova
I V
IV
Kormilitsina
M I
MI
Meshcheriakova
I S
IS
Verkhovskaia
L V
LV
Naroditskiĭ
B S
BS
Gintsburg
A L
AL
D000276:Adjuvants, Immunologic / Q000378:metabolism; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001386:Azides; D001421:Bacteria, Anaerobic / Q000201:enzymology; D001426:Bacterial Proteins / Q000276:immunology*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000276:immunology* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005604:Francisella tularensis / Q000276:immunology*; D005620:Freund's Adjuvant; D007115:Immunization Schedule; D007279:Injections, Subcutaneous; D008074:Lipoproteins / Q000276:immunology*; D018541:Neptune; D011485:Protein Binding; D017434:Protein Structure, Tertiary / Q000502:physiology; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology; D014406:Tularemia / Q000097:blood / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001386:Azides; D001426:Bacterial Proteins; D008074:Lipoproteins; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C075214:TUL4 protein, Francisella tularensis; D002482:Cellulose; D005620:Freund's Adjuvant; C087879:endoglucanase CelD; D002480:Cellulase; C031042:potassium azide