Surface plasmon resonance-enabled mass spectrometry arrays.
Biosensors that utilize surface plasmon resonance (SPR) as a method of detection of protein interactions can be used for selective separation of proteins prior to MS analysis. The combination of SPR and MS results in a unique multiplexed detection technology capable of both quantitative and qualitative protein analysis. To further the development of a high-throughput SPR-MS approach, the possibility of arraying binding ligands on SPR chips for affinity capture of proteins and their MS analysis was explored. Antibodies to beta-2-microglobulin, cystatin C, transferrin, and insulin-like growth factors I and II were arrayed on a large number of SPR chips. Human plasma samples were injected over the antibody array chips in an SPR Biosensor, after which on-chip MS analysis was performed to detect the bound proteins. Signals from the targeted proteins were observed for each antibody-derivatized chip, indicating successful antibody immobilization and protein capture. The SPR-MS arrays are robust, highly reproducible, and are capable of high-throughput analysis.
Nedelkov
Dobrin
D
Intrinsic Bioprobes Inc., Tempe, AZ 85281, USA. dnedelkov@intrinsicbio.com
Tubbs
Kemmons A
KA
Nelson
Randall W
RW
D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D013058:Mass Spectrometry; D040081:Protein Array Analysis / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D000906:Antibodies; D001798:Blood Proteins