[Flow fluorimetry in differential diagnosis of diffuse large B-cell lymphoma].
OBJECTIVE
To analyse immunophenotype of diffuse large B-cell lymphoma (DLBCL) with flow cytometry.


METHODS
Combinations of antibodies against the following antigens were used: CD3/ CD19/CD45, CD5/CD19/CD38, CD19/CD10/CD23, CD4/CD8/CD3, kappa/lambda/CD19, CD25/CD20/FMC7; CD43/CD22/CD20; CD79a/Ki-67/CD3; cytoplasmic kappa/lambda. The analysis was made on flow fluorimeter FacsCalibur using computer program CellQuest (Beckton Dickenson, USA).


RESULTS
Specific coexpression of markers is not detectable in DLBCL, in the greatest degree the phenotype corresponds to lymphoma from the cells of the marginal zone. The study of cells with maximal direct light diffusion provides more precise assessment of clonality and proliferative potential of tumor cells than the analysis of the whole lymphocytic polygon. The proliferative index in 33 cases of DLBCL varied in the range 10-60%.


CONCLUSIONS
Flow cytometry in most DLBCL cases allows identification of B-cell clonality, more precise assessment of a proliferative potential of the tumor.
Vorob'ev
I A
IA
Gorgidze
L A
LA
Gretsov
E M
EM
Korneva
E P
EP
Kharazishvili
D V
DV
Khudoleeva
O A
OA
Churakova
Zh V
ZhV
D000951:Antigens, Neoplasm / Q000032:analysis*; D003937:Diagnosis, Differential; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D016393:Lymphoma, B-Cell / Q000473:pathology*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology*
D000951:Antigens, Neoplasm