Differential immunogold localisation of sulphated and unsulphated keratan sulphate proteoglycans in normal and macular dystrophy cornea using sulphation motif-specific antibodies.
Keratan sulphate (KS) proteoglycans (PGs) are key molecules in the corneal stroma for tissue organisation and transparency. Macular corneal dystrophy (MCD) is a rare, autosomal recessive disease characterised by disturbances in KS expression. MCD is caused by mutations in CHST6, a gene encoding the enzyme responsible for KS sulphation. Sulphated KS is absent in type I disease causing corneal opacity and loss of vision. Genetic studies have highlighted the mutational heterogeneity in MCD, but supportive immunohistochemical studies on corneal KS have previously been limited by the availability of antibodies mostly reactive only with highly sulphated KS epitopes. In this study, we employed four antibodies against specific KS sulphation patterns, including one against unsulphated KS, to investigate their reactivity in a case of MCD compared with normal cornea using high-resolution immunogold electron microscopy. Mutation analysis indicated type I MCD with deletion of the entire open reading frame of CHST6. Contrast enhanced fixation revealed larger PG structures in MCD than normal. Unlike normal cornea, MCD cornea showed positive labelling with antibody to unsulphated KSPG, but was negative with antibodies to sulphated KSPG. These antibodies will thus facilitate high-resolution investigations of phenotypic heterogeneity in support of genetic studies in this disease.
Young
Robert D
RD
Structural Biophysics Group, School of Optometry & Vision Sciences, Cardiff University, Redwood Building, King Edward VII Avenue, Cardiff, CF10 3NB Wales, UK.
Akama
Tomoya O
TO
Liskova
Petra
P
Ebenezer
Neil D
ND
Allan
Bruce
B
Kerr
Briedgeen
B
Caterson
Bruce
B
Fukuda
Michiko N
MN
Quantock
Andrew J
AJ
D000328:Adult; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003317:Corneal Dystrophies, Hereditary / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007632:Keratan Sulfate / Q000032:analysis*; D008297:Male; D008854:Microscopy, Electron; D009154:Mutation; D011509:Proteoglycans / Q000032:analysis
D000911:Antibodies, Monoclonal; D011509:Proteoglycans; D007632:Keratan Sulfate