[Complementarity-determining region 3 analysis of T cell receptor beta chain variable region in peripheral blood mononuclear cells of patients with systemic lupus erythematosus].
OBJECTIVE
To analyze the drift of the complementarity-determining region 3 (CDR3) of T cell receptor (TCR) beta chain variable region (TCR BV) in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus.


METHODS
Immunoscope spectratyping techniques was used to analyze the distribution of TCRbeta chain CDR3 in 5 normal blood donors and the dominant CDR3 in the PBMCs in 5 SLE patients. Sequence analysis of the CDR3 region in monoclonal or oligoclonal T cells was performed.


RESULTS
The spectratypes of TCR BV gene CDR3 region showed Gaussian distribution in the 5 normal blood donors. The 5 SLE patients, however, displayed anomalous proliferation and oligoclonal expansion of the T cells was observed in different TCR BV families with different CDR3 sequences.


CONCLUSIONS
Noticeable drift of TCRbeta chain CDR3 can be seen in active SLE, indicating possible association of selective expression of TCR with immune pathogenesis in SLE. Determination of specific TCR CDR3 sequence provides a new means for studying the pathogenesis and personalized treatment of SLE.
Luo
Wei
W
Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China. LuoWei 421@163.com
Ma
Li
L
Yao
Xin-sheng
XS
Zou
Hong-yun
HY
Wen
Qian
Q
Ruan
Guang-ping
GP
Wang
Xiao-ning
XN
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D022801:Complementarity Determining Regions / Q000235:genetics*; D005260:Female; D019674:Genes, T-Cell Receptor beta / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D022801:Complementarity Determining Regions