Soluble forms of YlCrh1p and YlCrh2p, cell wall proteins of Yarrowia lipolytica, have beta-1,3-glycosidase activity.
Crh1p and Crh2p of Saccharomyces cerevisiae are cell wall proteins covalently attached to cell wall glucan and are thought to be putative glycosidases involved in cell wall remodelling. We investigated whether YlCrh1p and YlCrh2p, the Yarrowia lipolytica proteins homologous to ScCrh1p and ScCrh2p, had the required glycosidase activity for cell wall biosynthesis and maintenance. Ylcrh1Delta and Ylcrh2Delta mutants showed sensitivity to compounds that interfere with cell wall construction. Soluble forms of YlCrh1p and YlCrh2p that lacked the C-terminal consensus sequence for GPI anchoring showed glycosidase activity on laminarin, a substrate carrying beta-1,3-glycosidic linkage. Our study suggests that the YlCrh1p and YlCrh2p may participate in cell wall biosynthesis and remodelling through their beta-1,3-glycosidase activity.
Hwang
Ji-Sook
JS
Institute of Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.
Seo
Dong-Hyuck
DH
Kim
Jeong-Yoon
JY
D000595:Amino Acid Sequence; D001557:Benzenesulfonates; D002473:Cell Wall / Q000201:enzymology / Q000378:metabolism; D003224:Congo Red; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D012995:Solubility; D025062:Yarrowia / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D000099052:CRF Receptor, Type 1
D001557:Benzenesulfonates; D005656:Fungal Proteins; D018019:Receptors, Corticotropin-Releasing Hormone; D003224:Congo Red; D000099052:CRF Receptor, Type 1; C007061:C.I. Fluorescent Brightening Agent 28; D006026:Glycoside Hydrolases