title: In vitro assessment of the effect of interleukin-1beta on angiogenic potential of bone marrow cells.
abstract: BACKGROUND
  Therapeutic angiogenesis for ischemic diseases has been successfully induced by the implantation of autologous bone marrow cells (BMCs). It is understood that interleukin (IL)-1beta increases remarkably in ischemic tissue and has particular effects on angiogenesis. Thus, it is important to clarify how IL-1beta would effect BMCs survival and angiogenic potential.
  
  
  RESULTS
  The effect of IL-1beta on BMCs survival, adhesion, and endothelial differentiation, as well as the production of angiogenic growth factors, was investigated using an in vitro assessment approach. BMCs were harvested from Zucker obese rats and cultured at a density of 3x10(6) cells/ml with 5 ng/ml IL-1 beta (IL-1beta group) or without IL-1 beta (control group). Survival and adhesion of BMCs were significantly increased in the IL-1beta group than in the control group after 1, 3, and 7 days of culture (p<0.01). The release of vascular endothelial growth factor in supernatant was also significantly higher in the IL-1beta group than in the control group after 3 and 7 days of culture (p<0.01). Furthermore, the number of differentiated endothelial cells derived from BMCs was significantly higher in the IL-1beta group than in the control group after 7 days of culture (p<0.01).
  
  
  CONCLUSIONS
  These results suggest that IL-1beta has a positive effect on the angiogenic potential of BMCs in vitro.
authors.affiliation: Department of Medical Bioregulation, Division of Cardiovascular Surgery, Yamaguchi University School of Medicine, Japan.
authors.forename: Shu-Lan
authors.initials: SL
authors.lastname: Qin
authors.forename: Tao-Sheng
authors.initials: TS
authors.lastname: Li
authors.forename: Masaya
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kimikazu
authors.initials: K
authors.lastname: Hamano
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D007375:Interleukin-1 / Q000494:pharmacology*; D007511:Ischemia / Q000628:therapy; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D051381:Rats; D011924:Rats, Zucker
chemical_list: D007375:Interleukin-1