title: Evaluation of cellulase preparations for hydrolysis of hardwood substrates.
abstract: Seven cellulase preparations from Penicillium and Trichoderma spp. were evaluated for their ability to hydrolyze the cellulose fraction of hardwoods (yellow poplar and red maple) pretreated by organosolv extraction, as well as model cellulosic substrates such as filter paper. There was no significant correlation among hydrolytic performance on pretreated hardwood, based on glucose release, and filter paper activity. However, performance on pretreated hardwood showed significant correlations to the levels of endogenous beta-glucosidase and xylanase activities in the cellulase preparation. Accordingly, differences in performance were reduced or eliminated following supplementation with a crude beta-glucosidase preparation containing both activities. These results complement a previous investigation using softwoods pretreated by either organosolv extraction or steam explosion. Cellulase preparations that performed best on hardwood also showed superior performance on the softwood substrates.
authors.affiliation: Forest Products Biotechnology, Faculty of Forestry, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada. alex.berlin@ubc.ca
authors.forename: Alex
authors.initials: A
authors.lastname: Berlin
authors.forename: Neil
authors.initials: N
authors.lastname: Gilkes
authors.forename: Douglas
authors.initials: D
authors.lastname: Kilburn
authors.forename: Vera
authors.initials: V
authors.lastname: Maximenko
authors.forename: Renata
authors.initials: R
authors.lastname: Bura
authors.forename: Alexander
authors.initials: A
authors.lastname: Markov
authors.forename: Anton
authors.initials: A
authors.lastname: Skomarovsky
authors.forename: Alexander
authors.initials: A
authors.lastname: Gusakov
authors.forename: Arkady
authors.initials: A
authors.lastname: Sinitsyn
authors.forename: Oleg
authors.initials: O
authors.lastname: Okunev
authors.forename: Irina
authors.initials: I
authors.lastname: Solovieva
authors.forename: John N
authors.initials: JN
authors.lastname: Saddler
mesh_terms: D002480:Cellulase / Q000737:chemistry* / Q000145:classification*; D002482:Cellulose / Q000737:chemistry*; D003198:Computer Simulation; D004789:Enzyme Activation; D006868:Hydrolysis; D007700:Kinetics; D008954:Models, Biological; D008956:Models, Chemical; D013379:Substrate Specificity; D014197:Trees / Q000737:chemistry*; D014934:Wood
chemical_list: D002482:Cellulose; D002480:Cellulase