[Determination of urinary trans, trans-muconic acid by high performance liquid chromatography].
A method for the determination of urinary trans, trans-muconic acid (tt-MA) (benzene metabolite) by high performance liquid chromatography (HPLC) was developed. The separation was carried out on a C18 column (Spherisorb C18, 150 mm x 4.6 mm i. d. , 5 microm) at 25 degrees C with glacial acetic acid-tetrahydrofuran-methanol-water (1 : 2 : 10: 87, v/v) as mobile phase. Urinary sample was acidified by 2 mol/L hydrochloric acid and pretreated by liquid-liquid extraction using diethyl ether. After removal of diethyl ether with a stream of nitrogen, the residue was re-dissolved in 1 mL of mobile phase for HPLC injection. Good linearity was observed within the range from 0.10 mg/L to 10.00 mg/L (r =0.9999) and the detection limit was 0.10 mg/L. The average recoveries for tt-MA were 95.1%-100.5%. Relative standard deviations (RSD) for intra-day and inter-day determinations were 4.0%-9.0% and 6.2%-8.8%, respectively. The method was applied to 56 benzene-exposed workers and 24 controll workers. Urinary tt-MA in benzen-exposed workers were significantly higher than that in the control group. They can be correlated with benzene exposure concentrations (P <0.01). This analytical method for tt-MA is sensitive, rapid, and convenient. It is suitable for monitoring of occupational exposure to benzene and toxic kinetics studies.
Liu
Liwen
L
Wuhan University of Science and Technology, China. llw952@hotmail.com
Song
Shizhen
S
Hu
Xiamin
X
Ye
Fangli
F
D000395:Air Pollutants, Occupational / Q000652:urine*; D000818:Animals; D001554:Benzene / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D016273:Occupational Exposure / Q000032:analysis*; D012015:Reference Standards; D013011:Sorbic Acid / Q000031:analogs & derivatives* / Q000032:analysis
D000395:Air Pollutants, Occupational; C005939:muconic acid; D001554:Benzene; D013011:Sorbic Acid