title: Cerebellum progesterone concentration decreased in canine distemper virus infection.
abstract: Progesterone has neuroprotective effects including augmentation of myelination in the central and peripheral nervous system. This study was designed to determine if demyelinating lesions in the cerebellum resulting from canine distemper virus (CDV) infection are associated with progesterone levels. Progesterone was measured using radioimmunoassay in samples of the cerebellum, corpus callosum, medulla oblongata, parietal, frontal, temporal, and occipital cortices as well as cerebrospinal fluid (CSF) and plasma collected from ten CDV infected and six non-infected dogs. The cerebellum progesterone level was significantly different between CDV infected (0.66+/-0.09 ng/g) and control dogs (1.14+/-0.09 ng/g) (p<0.001); however, no difference was observed for the other CNS regions, plasma and CSF (p>0.05). The cerebellum progesterone level was also significantly different between acute (0.71+/-0.0 5 ng/g) and chronic cases (0.61+/-0.09 ng/g) (p<0.05). The CDV infected cerebella were also categorized histopathologically according to the severity of demyelinating lesions as mild (n=5), moderate (n=2), or severe (n=3) among which the cerebellum progesterone level was significantly different (p<0.05). Progesterone concentration was 0.71+/-0.05 ng/g in mild, 0.65+/-0.10 ng/g in moderate, and 0.56+/-0.07 ng/g in severe cases. In conclusion, progesterone concentration decreases in the cerebellum in CDV infection and the severity of demyelinating lesions is the greatest in cerebella with the lowest progesterone concentrations. The results suggest that local impairment of progesterone metabolism may be associated with the initiation and progression of cerebellar lesions in CDV infection.
authors.affiliation: Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, 55139 Kurupelit, Samsun, Turkey. gulayarim@omu.edu.tr
authors.forename: Gul Fatma
authors.initials: GF
authors.lastname: Yarim
authors.forename: Siyami
authors.initials: S
authors.lastname: Karahan
authors.forename: Murat
authors.initials: M
authors.lastname: Yarim
mesh_terms: D000818:Animals; D020805:Central Nervous System Viral Diseases / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D002531:Cerebellum / Q000378:metabolism* / Q000821:virology; D004216:Distemper / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism* / Q000821:virology; D004217:Distemper Virus, Canine / Q000254:growth & development*; D004285:Dogs; D005260:Female; D006651:Histocytochemistry / Q000662:veterinary; D008297:Male; D011374:Progesterone / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D018709:Statistics, Nonparametric
chemical_list: D011374:Progesterone