title: Global gene expression analyses of mouse fibroblast L929 cells exposed to IC50 MMA by DNA microarray and confirmation of four detoxification genes' expression by real-time PCR.
abstract: Methyl methacrylate (MMA) is the main component of methyl methacrylic resin, which is widely used in dentistry. Previous studies have investigated whether MMA has any adverse effects on growth and gene expression in mouse fibroblast L929 cells. The present study was designed to further understand the effects of MMA by focusing on cDNA microarray data after L929 cells were exposed to MMA. MMA was found to inhibit cell growth and induce detoxification response genes in L929 cells. One of the most highly up-regulated genes was glutathione S-transferase, alpha 1 (Ya) (Gsta1), which has recently been shown to participate in Nrf2 regulation and is considered to be related to detoxification response. Molecular biological data obtained in the present study may therefore provide useful insights into the effects of MMA on living tissue.
authors.affiliation: Second Department of Prosthodontics, School of Dentistry, Aichi-gakuin University, 2-11 Suemori-dori, Chikusaku, Nagoyashi, Aichi-ken 464-8651, Japan. issy@dpc.aichi-gakuin.ac.jp
authors.forename: Atsuko
authors.initials: A
authors.lastname: Ishikawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Jinno
authors.forename: Tomoo
authors.initials: T
authors.lastname: Suzuki
authors.forename: Tatsuhide
authors.initials: T
authors.lastname: Hayashi
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Kawai
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Mizuno
authors.forename: Takashi
authors.initials: T
authors.lastname: Mori
authors.forename: Masami
authors.initials: M
authors.lastname: Hattori
mesh_terms: D000818:Animals; D049108:Cell Enlargement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003764:Dental Materials / Q000633:toxicity*; D004789:Enzyme Activation; D005347:Fibroblasts / Q000187:drug effects; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics; D008658:Inactivation, Metabolic / Q000235:genetics*; D020128:Inhibitory Concentration 50; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D007739:L Cells; D020366:Methylmethacrylate / Q000633:toxicity*; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D003764:Dental Materials; D007527:Isoenzymes; D051267:NF-E2-Related Factor 2; D020366:Methylmethacrylate; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha