title: Distinct sources and targets of IL-10 during dendritic cell-driven Th1 and Th2 responses in vivo.
abstract: Dendritic cells (DC) can both initiate an immune response and dictate its character. Cytokines are critically involved in this process and, although interleukin (IL)-10 is known as a potent immunosuppressant, the impact of its release from DC remains unclear. Here, we transfer pathogen-conditioned murine DC in vivo and show that, while DC-derived IL-10 can act to limit Th1 development, it is not required for Th2 induction. In both Th2 and Th1 settings, however, IL-10 from cells other than the initiating DC dominates the regulation of the emerging effector cell populations. Surprisingly, the critical source of IL-10 in this process is neither T nor B cells. These data illustrate the distinct actions of IL-10 during differently polarised, pathogen-focussed, DC-driven immune responses in vivo.
authors.affiliation: Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
authors.forename: Georgia
authors.initials: G
authors.lastname: Perona-Wright
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Jenkins
authors.forename: Alison
authors.initials: A
authors.lastname: Crawford
authors.forename: David
authors.initials: D
authors.lastname: Gray
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Pearce
authors.forename: Andrew S
authors.initials: AS
authors.lastname: MacDonald
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D016753:Interleukin-10 / Q000172:deficiency / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D000947:Antigens, Helminth; D016753:Interleukin-10