[Construction of HPV type 16 Li antigen expressing tumor model].
Recombinant expression vector was constructed by techniques of gene recombination, and identified by restriction endonuclease and sequence analysis. Then the recombinant was transfected into B16 cell by techniques of gene transfection and expressions were detected by RT-PCR and IFA. After that, transfected cells were inoculated into subcutaneous of mouse and the forming tumor and expression of HPV16L1 protein after tumor was formed was observed. Identification of pcDNA- HPV16L1 by enzyme digestion showed that the length, inserted location and direction of the target gene which was inserted into the recombinant was correct and the expression of L1 in transfected cell was observed by IFA. The inoculated cells could form tumor in vivo obviously and HPV16 L1 protein could express in the cells stably.
Gao
Hui
H
Comparative Medicine Central of Yangzhou University, Yangzhou 225009.
Huang
Zheng Fang
ZF
Liu
Jin
J
Xue
Zheng Feng
ZF
Li
Hou Da
HD
D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D052162:Human papillomavirus 16 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D036022:Capsid Proteins; D009856:Oncogene Proteins, Viral; C063317:L1 protein, Human papillomavirus type 16