Determination of nitroimidazole residues in porcine urine by liquid chromatography/tandem mass spectrometry.
A reliable and sensitive method was developed for determination of nitroimidazoles in porcine urine. Sample preparation involves an extraction with ethyl acetate, followed by a solid-phase extraction cleanup step. The final extract is injected into the liquid chromatography/tandem mass spectrometry system in the positive-ion electrospray ionization mode. A C8 column with water and acetonitrile as the mobile phase is used for chromatographic separation under gradient conditions. Overall average recoveries ranged from 83 to 107%. The limits of detection ranged from 0.03 to 0.05 ng/mL, and the limits of quantitation, from 0.1 to 0.2 ng/mL.
Xia
Xi
X
China Agricultural University, College of Veterinary Medicine, Department of Pharmacology and Toxicology, Beijing, 100094, People's Republic of China.
Li
Xiaowei
X
Shen
Jianzhong
J
Zhang
Suxia
S
Ding
Shuangyang
S
Jiang
Haiyang
H
D000818:Animals; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*; D009593:Nitroimidazoles / Q000032:analysis* / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013552:Swine; D013696:Temperature; D014556:Urine
D007477:Ions; D009593:Nitroimidazoles; D012997:Solvents