title: Crystal structure of the apo-PerR-Zn protein from Bacillus subtilis.
abstract: Bacteria adapt to elevated levels of Reactive Oxygen Species (ROS) by increasing the expression of defence and repair proteins, which is regulated by ROS responsive transcription factors. In Bacillus subtilis the zinc protein PerR, a peroxide sensor that binds DNA in the presence of a regulatory metal Mn2+ or Fe2+, mediates the adaptive response to H2O2. This study presents the first crystal structure of apo-PerR-Zn which shows that all four cysteine residues of the protein are involved in zinc co-ordination. The Zn(Cys)4 site locks the dimerization domain and stabilizes the dimer. Sequence alignment of PerR-like proteins supports that this structural site may constitute a distinctive feature of this class of peroxide stress regulators.
authors.affiliation: DRDC/Laboratoire de Physicochimie des Métaux en Biologie, UMR 5155 CEA-CNRS-UJF, CEA-Grenoble, 38054 Grenoble Cedex 9, France.
authors.forename: Daouda A K
authors.initials: DA
authors.lastname: Traoré
authors.forename: Abdelnasser
authors.initials: A
authors.lastname: El Ghazouani
authors.forename: Sougandi
authors.initials: S
authors.lastname: Ilango
authors.forename: Jérôme
authors.initials: J
authors.lastname: Dupuy
authors.forename: Lilian
authors.initials: L
authors.lastname: Jacquamet
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Ferrer
authors.forename: Christelle
authors.initials: C
authors.lastname: Caux-Thang
authors.forename: Victor
authors.initials: V
authors.lastname: Duarte
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Latour
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods*; D003545:Cysteine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019281:Dimerization; D004228:Dithionitrobenzoic Acid / Q000737:chemistry; D015964:Gene Expression Regulation, Bacterial; D008958:Models, Molecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015032:Zinc / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C122138:peroxide repressor proteins; D004228:Dithionitrobenzoic Acid; D015032:Zinc; D003545:Cysteine