Adiponectin-induced secretion of interleukin-6 (IL-6), monocyte chemotactic protein-1 (MCP-1, CCL2) and interleukin-8 (IL-8, CXCL8) is impaired in monocytes from patients with type I diabetes.
BACKGROUND
Systemic adiponectin is reduced in patients with cardiovascular disease (CVD) and low adiponectin may contribute to the pathogenesis of atherosclerosis. However, circulating adiponectin is elevated in type 1 diabetes (T1D) patients, who have also a higher incidence to develop CVD. Because monocytes play an important role in atherosclerosis, we analysed the influence of adiponectin on cytokine and chemokine release in monocytes from T1D patients and controls.


METHODS
Systemic adiponectin was determined in the plasma and the high-molecular weight (HMW) form of adiponectin was analysed by immunoblot. Monocytes were isolated from T1D patients and controls and the adiponectin-stimulated release of interleukin-6 (IL-6), monocyte chemotactic protein-1 (MCP-1, CCL2) and interleukin-8 (IL-8, CXCL8) was analysed.


RESULTS
Systemic adiponectin was higher in T1D patients. Immunoblot analysis of the plasma indicate abundance of HMW adiponectin in T1D patients and controls. IL-6, CCL2 and CXCL8 secretion in response to adiponectin were found induced in monocytes from controls whereas only IL-6 was upregulated in T1D cells. The induction of IL-6 by adiponectin was abrogated by an inhibitor of the NFkappaB pathway.


CONCLUSIONS
These data indicate that adiponectin-mediated induction of IL-6, CCL2 and CXCL8 is disturbed in monocytes from T1D patients and therefore elevated systemic adiponectin in T1D patients may be less protective when compared to controls.
Abke
Sabine
S
Department of Internal Medicine I, University of Regensburg, D-93042 Regensburg, Germany. abs507310@gw.ngate.uni-regensburg.de
Neumeier
Markus
M
Weigert
Johanna
J
Wehrwein
Gabriele
G
Eggenhofer
Elke
E
Schäffler
Andreas
A
Maier
Kevin
K
Aslanidis
Charalampos
C
Schölmerich
Jürgen
J
Buechler
Christa
C
D052242:Adiponectin / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D015992:Body Mass Index; D018932:Chemokine CCL2 / Q000097:blood* / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood* / Q000378:metabolism; D016209:Interleukin-8 / Q000097:blood* / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D012016:Reference Values
D052242:Adiponectin; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D015850:Interleukin-6; D016209:Interleukin-8; D011994:Recombinant Proteins