Generation of a reporter cell line for detection of infectious varicella-zoster virus and its application to antiviral studies.
To simplify the titration of infectious varicella-zoster virus (VZV), we generated a reporter cell line that produced luciferase in a dose-dependent manner upon infection with cell-free VZV. A few VZV-infected cells were detectable by coculturing with the cell line. We demonstrated the usefulness of the cell line for antiviral studies.
Wang
Guan-Qing
GQ
Department of Virology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.
Suzutani
Tatsuo
T
Yamamoto
Yumiko
Y
Fukui
Yoshiko
Y
Nozawa
Naoki
N
Schmid
D Scott
DS
Kurane
Ichiro
I
Inoue
Naoki
N
D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D002460:Cell Line / Q000201:enzymology* / Q000821:virology*; D017930:Genes, Reporter / Q000235:genetics; D014645:Herpesvirus 3, Human / Q000187:drug effects / Q000235:genetics*; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000379:methods*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic
D000998:Antiviral Agents; D008156:Luciferases