title: In vitro microspore selection in maize anther culture with oxidative-stress stimulators.
abstract: In order to produce doubled-haploid maize plants tolerant of oxidative stress, in vitro microspore selection was carried out in anther culture with reactive oxygen species (ROS) progenitors such as paraquat, menadione, tert-butylhydroperoxide (t-BHP), and methionine combined with riboflavin. All the ROS progenitors reduced the anther induction, the formation of microspore-derived structures, and their regeneration potential. Abnormal cell divisions and progeny cell degradation could be observed during the development of microspores treated with ROS progenitors. Menadione and t-BHP influenced the microspore developmental pathway, as menadione induced the formation of embryoids, while t-BHP increased the proportion of calli in the microspore-derived structures. As the result of in vitro selection, 15, 10, 10, and 3 fertile doubled-haploid plants were obtained in cultures treated with paraquat, t-BHP, methionine combined with riboflavin, and menadione, respectively.
authors.affiliation: Agricultural Research Institute, Hungarian Academy of Sciences, Martonvásár, Hungary.
authors.forename: H
authors.initials: H
authors.lastname: Ambrus
authors.forename: E
authors.initials: E
authors.lastname: Darko
authors.forename: L
authors.initials: L
authors.lastname: Szabo
authors.forename: F
authors.initials: F
authors.lastname: Bakos
authors.forename: Z
authors.initials: Z
authors.lastname: Király
authors.forename: B
authors.initials: B
authors.lastname: Barnabás
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D035264:Flowers / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012038:Regeneration / Q000187:drug effects; D012639:Seeds / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D003313:Zea mays / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species