[Expression of anti-HBc single-chain variable fragment mediated by recombinant replication defective adenovirus in vitro].
OBJECTIVE
To define the expression of single-chain variable fragment (ScFv) against hepatitis B virus core protein (HBc) mediated by recombinant replication defective adenovirus carrying the anti-HBc ScFv gene in vitro and to define the activity of anti-HBc ScFv combining HBcAg.


METHODS
The recombinant adenoviruses carrying anti-HBc ScFv gene generated by homologous recombination in bacteria and packaged in 293 cells were transfected into HepG2 cells, and the anti-HBc ScFv was detected using SDS-PAGE and Western blot.


RESULTS
Green fluorescent protein (GFP) was observed in HepG2 cells after the transfection. SDS-PAGE displayed a protein strap about 2.7 x 10(4), and the result of Western blot displayed a positive reactive strap.


CONCLUSIONS
Anti-HBc ScFv can be expressed in cells mediated by recombinant replication defective adenovirus carrying the anti-HBc ScFv gene.
Tang
Zheng-hao
ZH
Department of Infectious Diseases, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, China. tzhhao@hotmail.com
Yu
Yong-sheng
YS
Ma
Hui-hui
HH
Jiang
Hong
H
Xi
Min
M
Zang
Guo-qing
GQ
Yao
Ji-lu
JL
D000256:Adenoviridae / Q000235:genetics*; D002460:Cell Line; D006510:Hepatitis B Antibodies / Q000235:genetics* / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D057127:Single-Chain Antibodies / Q000235:genetics* / Q000276:immunology*; D014162:Transfection
D006510:Hepatitis B Antibodies; D057127:Single-Chain Antibodies