title: Investigation of Musashi-1 expressing cells in the murine model of dextran sodium sulfate-induced colitis.
abstract: Musashi-1 (Msi-1), an RNA-binding protein, had been proposed to be a specific marker for neural stem/precursor cells. Msi-1 expressing cells in the intestinal epithelium are also strongly considered as potential stem/precursor cells. To clarify the behavior of those cells in the injury or regeneration phase, we investigated Msi-1 expressing cells of intestinal mucosa in the murine model of dextran sodium sulfate (DSS)-induced colitis. Immunohistochemically, Msi-1-positive cells were found in the area just along the layer of Paneth's cells in the small intestine and in the bottom layer of crypts in the large intestine. During DSS administration, the number of PCNA-positive cells in the large intestine increased markedly. In contrast, the number of Msi-1-positive cells decreased slightly with DSS but returned to normal after DSS administration was stopped. The level of mRNA for Msi-1 was consistent with the result of immunohistochemical examinations. Conclusively, we could describe the behavior of intestinal stem/precursor cells during inflammation using Msi-1.
authors.affiliation: Department of Gastroenterology, Yamagata University School of Medicine, 2-2-2 Iida-Nishi, Yamagata, Japan, 990-9585.
authors.forename: Tadahisa
authors.initials: T
authors.lastname: Fukui
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takeda
authors.forename: Hong-Jin
authors.initials: HJ
authors.lastname: Shu
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Ishihama
authors.forename: Sayaka
authors.initials: S
authors.lastname: Otake
authors.forename: Yasukuni
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Shoichi
authors.initials: S
authors.lastname: Nishise
authors.forename: Nanami
authors.initials: N
authors.lastname: Ito
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sato
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Togashi
authors.forename: Sumio
authors.initials: S
authors.lastname: Kawata
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000378:metabolism* / Q000473:pathology; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*
chemical_list: C104786:Msi1h protein, mouse; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D016264:Dextran Sulfate