Analysis of polymerase chain reaction products by denaturing high-performance liquid chromatography.
Denaturing high-performance liquid chromatography (DHPLC) analysis is an ion-pair reversed-phase high performance liquid chromatography for performing analytical separations of DNA based on temperature: analysis is performed at a temperature sufficient to partially denature DNA heteroduplexes. The technology detects single-base changes as efficiently as short deletions and insertions. The chance that a mutation cannot be detected is 0.5%.
Lam
Ching-Wan
CW
Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR.
D001331:Automation; D001483:Base Sequence; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005091:Exons; D005260:Female; D006720:Homozygote; D006801:Humans; D008297:Male; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature