Gene expression profiles of O3-treated Arabidopsis plants.
To analyse cellular response to O(3), the tolerant Arabidopsis thaliana genotype Col-0 was exposed to O(3) fumigation (300 ppb) for 6 h and the modulation of gene expression during the treatment (3 h after the beginning of the treatment, T3 h) and the recovery phase (6 h from the end of the treatment, T12 h) assessed by gene chip microarray and real-time reverse transcriptase (RT)-PCR analyses. The Arabidopsis transcriptional profile is complex, as new genes (i.e. reticuline oxidase) and pathways, other than those already reported as O(3)-responsive, appear to be involved in the O(3) response. The steady-state transcript levels of several WRKY genes were increased in O(3)-treated plants and the W-box was the cis-element over-represented in the promoter region of T3 h up-regulated genes. The fact that the W-box element was also over-represented in almost all T3 h-induced receptor-like kinases (RLKs) suggests a WRKY-mediated control of RLKs under O(3) stress and a mechanicistic similarity with the pathogen-induced transcriptional responses. We investigated the molecular and physiological implications of our findings in relation to O(3)-induced plant stress response.
Tosti
Nicola
N
Università degli Studi di Perugia, Dipartimento di Biologia Vegetale e Biotecnologie Agroambientali e Zootecniche, Borgo XX Giugno, 74, I-06121 Perugia, Italy.
Pasqualini
Stefania
S
Borgogni
Andrea
A
Ederli
Luisa
L
Falistocco
Egizia
E
Crispi
Stefania
S
Paolocci
Francesco
F
D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D015536:Down-Regulation / Q000187:drug effects; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D010126:Ozone / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
D029681:Arabidopsis Proteins; D010126:Ozone