Inhibition of human immunodeficiency virus type 1 entry by a binding domain of Porphyromonas gingivalis gingipain.
Human immunodeficiency virus (HIV) transmission through saliva is extremely low. Several oral components, including secretory immunoglobulin A and secretory leukocyte protease inhibitor, are known as potential inhibitory agents of HIV oral transmission. Here we examined anti-HIV activity of oral bacterial components. We showed that recombinant protein HGP44 derived from Porphyromonas gingivalis, one of the primary infectious agents of periodontitis, was capable of inhibiting HIV type 1 (HIV-1) replication. HGP44 bound specifically to HIV-1 gp120 and blocked HIV-1 envelope-mediated membrane fusion. These findings suggest that HGP44 of P. gingivalis can inhibit HIV-1 infection by blocking HIV-1 entry.
Xie
Hua
H
School of Dentistry, Meharry Medical College, Nashville, TN 37208, USA. hxie@mmc.edu
Belogortseva
Natalya I
NI
Wu
Jie
J
Lai
Wei-Hong
WH
Chen
Chin-ho
CH
D018829:Adhesins, Bacterial / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001665:Binding Sites; D019556:COS Cells; D002522:Chlorocebus aethiops; D003546:Cysteine Endopeptidases / Q000302:isolation & purification / Q000494:pharmacology*; D000080867:Gingipain Cysteine Endopeptidases; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D015658:HIV Infections / Q000517:prevention & control / Q000821:virology; D015497:HIV-1 / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D010446:Peptide Fragments / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000201:enzymology*; D017434:Protein Structure, Tertiary; D014779:Virus Replication / Q000187:drug effects
D018829:Adhesins, Bacterial; D000998:Antiviral Agents; D000080867:Gingipain Cysteine Endopeptidases; D015699:HIV Envelope Protein gp120; D010446:Peptide Fragments; D003546:Cysteine Endopeptidases