Modification of injection mapping of vascular-capillary bed in cerebral nervous tissue.
A simple original method of simultaneous staining of the cell elements on cerebral cortex and its vascular bed is developed. It is based on perfusion of polyvinyl acetate emulsion prepared on 2% paraformaldehyde solution with phosphate buffer at pH 7.4.
Ryabov
S I
SI
Laboratory of Ultrastructural and Cytochemical Principles of Conditioned Reflex, Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow. ryabov_si@inbox.ru
Kositsyn
N S
NS
D000818:Animals; D001808:Blood Vessels / Q000033:anatomy & histology / Q000502:physiology*; D002021:Buffers; D002196:Capillaries; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000098:blood supply*; D002560:Cerebrovascular Circulation; D004655:Emulsions; D005557:Formaldehyde / Q000008:administration & dosage; D006863:Hydrogen-Ion Concentration; D007276:Injections, Intraventricular / Q000379:methods; D010477:Perfusion / Q000379:methods*; D010710:Phosphates / Q000008:administration & dosage; D011108:Polymers / Q000008:administration & dosage; D011145:Polyvinyls / Q000008:administration & dosage*; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions
D002021:Buffers; D004655:Emulsions; D010710:Phosphates; D011108:Polymers; D011145:Polyvinyls; D012996:Solutions; D005557:Formaldehyde; C013215:polyvinyl acetate; C003043:paraform