title: [Predominance and evolution of BLA(SHV) genes in Tunisian hospital isolates of Klebsiella pneumoniae].
abstract: Extended-spectrum beta-lactamases (ESBLs)produced by clinical strains of Klebsiella pneumoniae were investigated, using isoelectric-focusing and DNA amplification followed by sequencing. A predominance of SHV variants was found. Sequencing identified the genes for the SHV-2a and -12 enzymes, suggesting direct evolution of SHV-12 from SHV-2a.
authors.affiliation: Laboratoire Interactions Légumineuses-Microorganismes, INRST, Hammam-Lif, Tunisie.
authors.forename: T
authors.initials: T
authors.lastname: Ben-Hamouda
authors.forename: K
authors.initials: K
authors.lastname: Ben-Mahrez
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics*; D019143:Evolution, Molecular; D014644:Genetic Variation / Q000235:genetics; D006786:Hospitals, Urban; D006801:Humans; D007525:Isoelectric Focusing; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics*; D017720:Molecular Epidemiology; D009154:Mutation / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D017422:Sequence Analysis, DNA; D014169:Transformation, Bacterial / Q000235:genetics; D014416:Tunisia / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; C054882:beta-lactamase PIT-2; C107198:beta-lactamase SHV-12; C062760:beta-lactamase SHV-2; C490029:beta-lactamase SHV-2a; C117193:beta-lactamase SHV-5; D001618:beta-Lactamases