A simple method to obtain en face sections of flat tissue culture cells.
A method is described for embedment of multiple confluent sheets of flat tissue culture cells that permits sectioning for thin or semithin sections in precise planes. The technique is especially useful for obtaining en face sections.
Piras
Monica
M
Department of Cytomorphology, School of Medicine, University of Cagliari, Cagliari, Italy.
Diaz
Giacomo
G
Tandler
Bernard
B
Bernardini
Silvana
S
Cossu
Margherita
M
Lantini
Maria S
MS
Falchi
Angela M
AM
Melis
Marta
M
Piludu
Marco
M
D002478:Cells, Cultured; D008853:Microscopy; D046529:Microscopy, Electron, Transmission; D008867:Microtomy / Q000379:methods*; D016610:Tissue Embedding / Q000379:methods*