Identification of differentially expressed genes in scarless wound healing utilizing polymerase chain reaction-suppression subtractive hybridization.
Wound healing in fetal skin is well known to proceed without scarring, whereas adult (postnatal) skin wound healing is accompanied by scar formation. To identify differentially expressed genes during fetal wound (FW) healing, we have used polymerase chain reaction-suppression subtractive hybridization. This technique allows for a comparative analysis across the entire transcriptome of FW vs. unwounded fetal control tissue, including even potentially novel sequences. Our subtractive hybridization protocol identified 15 clones that are overexpressed in healing FWs, and 20 clones that are underexpressed. These include genes with both known and unknown functions. We have confirmed the differential pattern of expression for four of these candidate genes: elongation factor 1 alpha, elongation initiation factor 4e, and two transcripts thus far known only as an expressed sequence tags. With this approach, we have also identified novel genes potentially involved in scarless wound healing.
Kathju
Sandeep
S
Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212-4772, USA. skathju@wpahs.org
Satish
Latha
L
Rabik
Cara
C
Rupert
Terra
T
Oswald
Duane
D
Johnson
Sandra
S
Hu
Fen Ze
FZ
Post
J Christopher
JC
Ehrlich
Garth D
GD
D000818:Animals; D002921:Cicatrix / Q000235:genetics*; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics / Q000378:metabolism; D020224:Expressed Sequence Tags / Q000378:metabolism; D005333:Fetus; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011817:Rabbits; D012867:Skin / Q000293:injuries*; D014945:Wound Healing / Q000235:genetics*; D014950:Wounds, Penetrating / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*
D039561:Eukaryotic Initiation Factor-4E; D020648:Peptide Elongation Factor 1