Gel-based hippocampal proteomic analysis 2 weeks following traumatic brain injury to immature rats using controlled cortical impact.
Traumatic brain injury (TBI) to postnatal day 17 rats has been shown to produce acute changes in hippocampal global protein levels and spatial learning and memory deficits. The purpose of the present study was to analyze global hippocampal protein changes 2 weeks after a moderate ipsilateral controlled cortical impact in postnatal day 17 rats using 2-dimensional difference gel electrophoresis and mass spectrometry. Paired sham and ipsilateral injured hippocampal lysates were independently labeled with different fluorescent cyanine dyes and coseparated within the same immobilized pH gradient strips and slab gel based on isoelectric point and molecular mass. Significant changes in key proteins involved in glial and neuronal stress, oxidative metabolism, calcium uptake and neurotransmitter function were found 2 weeks after injury, and their potential roles in hippocampal plasticity and cognitive dysfunction were discussed.
Kochanek
Ashley R
AR
Neurological Surgery, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Kline
Anthony E
AE
Gao
Wei-Min
WM
Chadha
Mandeep
M
Lai
Yichen
Y
Clark
Robert S B
RS
Dixon
C Edward
CE
Jenkins
Larry W
LW
D000375:Aging / Q000502:physiology; D000818:Animals; D001930:Brain Injuries / Q000378:metabolism* / Q000503:physiopathology*; D020013:Calcium Signaling / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002540:Cerebral Cortex / Q000293:injuries*; D002648:Child; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005456:Fluorescent Dyes; D006624:Hippocampus / Q000293:injuries / Q000378:metabolism* / Q000503:physiopathology*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008569:Memory Disorders / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000502:physiology; D018377:Neurotransmitter Agents / Q000032:analysis / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology
D005456:Fluorescent Dyes; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents