Computer simulation of wild-type and mutant human cardiac Na+ current.
Long QT syndrome (LQTS) and Brugada syndrome (BrS) are inherited diseases predisposing to ventricular arrhythmias and sudden death. Genetic studies linked LQTS and BrS to mutations in genes encoding for cardiac ion channels. Recently, two novel missense mutations at the same codon in the gene encoding the cardiac Na+ channel (SCN5A) have been identified: Y1795C (causing the LQTS phenotype) and Y1795H (causing the BrS phenotype). Functional studies in HEK293 cells showed that both mutations alter the inactivation of Na+ current and cause a sustained Na+ current upon depolarisation. In this paper, a nine state Markov model was used to simulate the Na+ current in wild-type Na+ cardiac channel and the current alterations observed in Y1795C and Y1795H mutant channels. The model includes three distinct closed states, a conducting open state and five inactivation states (one fast-, two intermediate- and two closed-inactivation). Transition rates between these states were identified on the basis of previously published voltage-clamp experiments. The model was able to reproduce the experimental Na+ current in mutant channels just by altering the assignment of model parameters with respect to wild-type case. Parameter assignment was validated by performing action potential clamp experiments and comparing experimental and simulated I(Na) current. The Markov model was subsequently introduced in the Luo-Rudy model of ventricular myocyte to investigate "in silico" the consequences on the ventricular cell action potential of the two mutations. Coherently with their phenotypes, the Y1795C mutation prolongs the action potential, while the Y1795H mutation causes only negligible changes in action potential morphology.
Vecchietti
Stefania
S
Cellular and Molecular Engineering Laboratory, DEIS, University of Bologna, via Venezia 52, 47023 Cesena (FC), Italy. svecchietti@deis.unibo.it
Rivolta
Ilaria
I
Severi
Stefano
S
Napolitano
Carlo
C
Priori
Silvia G
SG
Cavalcanti
Silvio
S
D000200:Action Potentials; D053840:Brugada Syndrome / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D003198:Computer Simulation; D006801:Humans; D008133:Long QT Syndrome / Q000235:genetics / Q000378:metabolism; D008390:Markov Chains; D008954:Models, Biological; D020125:Mutation, Missense; D018408:Patch-Clamp Techniques; D015222:Sodium Channels / Q000235:genetics* / Q000378:metabolism*
D015222:Sodium Channels