title: Functional coassembly of KCNQ4 with KCNE-beta- subunits in Xenopus oocytes.
abstract: The KCNQ gene family comprises voltage-gated potassium channels expressed in epithelial tissues (KCNQ1, KCNQ5), inner ear structures (KCNQ1, KCNQ4) and the brain (KCNQ2-5). KCNQ4 is expressed in inner and outer hair cells of the inner ear where it influences electrical excitability and cell survival. Accordingly, loss of function mutations of the KCNQ4 gene cause hearing loss in humans and functional k.o.-mice show progressive degeneration of outer hair cells (OHCs). However, characteristic electrophysiological features of the native KCNQ4- carried current I(K,n) in OHCs are not recapitulated by expression of KCNQ4 channels in heterologous expression systems. This might suggest modulation of KCNQ4 by interacting KCNE Beta-subunits, which are known to modify the properties of the closely related KCNQ1. The present study explored whether transcripts of the KCNE isoforms could be identified in OHC mRNA and whether the subunits modulate KCNQ4 function. RT-PCR indeed yielded transcripts of all five KCNEs in OHCs. Coexpression of the KCNE- Beta-subunits with human KCNQ4 in the Xenopus laevis oocyte expression system revealed that all KCNEs modulate KCNQ4 voltage dependence, protein stability and ion selectivity of hKCNQ4 in Xenopus oocytes. The deafness-associated Jervell and Lange- Nielsen syndrome (JLNS) mutation KCNE1(D76N) impairs KCNQ4-function whereas the Romano-Ward syndrome (RWS) mutant KCNE1(S74L), which shows normal hearing in patients, does not impair KCNQ4 channel function. In conclusion, KCNEs are presumably coexpressed with KCNQ4 in hair cells from the organ of Corti and might regulate KCNQ4 functional properties, effects that could be important under physiological and pathophysiological conditions.
authors.affiliation: Department of Physiology, University of Tuebingen, Tuebingen, Germany.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Strutz-Seebohm
authors.forename: Guiscard
authors.initials: G
authors.lastname: Seebohm
authors.forename: Olga
authors.initials: O
authors.lastname: Fedorenko
authors.forename: Ravshan
authors.initials: R
authors.lastname: Baltaev
authors.forename: Jutta
authors.initials: J
authors.lastname: Engel
authors.forename: Martina
authors.initials: M
authors.lastname: Knirsch
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005260:Female; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism / Q000502:physiology; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology; D029593:Jervell-Lange Nielsen Syndrome / Q000235:genetics / Q000503:physiopathology; D051656:KCNQ Potassium Channels / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D009154:Mutation / Q000235:genetics; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029597:Romano-Ward Syndrome / Q000235:genetics / Q000503:physiopathology; D014982:Xenopus laevis
chemical_list: D051656:KCNQ Potassium Channels; C117367:KCNQ4 protein, human; D024642:Potassium Channels, Voltage-Gated; D020033:Protein Isoforms