Molecular characterization of novel G5 bovine rotavirus strains.
Group A rotaviruses are a major cause of acute gastroenteritis in young children as well as many domestic animals. The rotavirus genome is composed of 11 segments of double-stranded RNA and can undergo genetic reassortment during mixed infections, leading to progeny viruses with novel or atypical phenotypes. The aim of this study was to determine if the bovine group A rotavirus strains KJ44 and KJ75, isolated from clinically infected calves, share genetic features with viruses obtained from heterologous species. All 11 genes sequences of the KJ44 and KJ75 strains were sequenced and analyzed. The KJ44 VP4 had 91.7% to 96.3% deduced amino acid identity to the bovine related P[1] strain, whereas the KJ75 strain was most closely related to the bovine related P[5] strain (91.9% to 96.9% amino acid identity). Both KJ44 and KJ75 strains also contained the bovine related VP3 gene. The remaining 9 segments were closely related to porcine group A rotaviruses. The KJ44 and KJ75 strains showed high amino acid identity to the G5 rotaviruses, sharing 90.4% to 99.0% identity. In addition, these strains belonged to the NSP4 genotype B, which is typical of porcine rotaviruses and subgroup I, with the closest relationship to the porcine JL-94 strain. These results strongly suggest that bovine rotavirus strains with the G5 genotype occur in nature as a novel G genotype in cattle as a result of a natural reassortment between bovine and porcine strains.
Park
Sung-Hee
SH
Bio-therapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea.
Saif
Linda J
LJ
Jeong
Cheol
C
Lim
Guem-Ki
GK
Park
Sang-Ik
SI
Kim
Ha-Hyun
HH
Park
Su-Jin
SJ
Kim
You-Jung
YJ
Jeong
Jae-Ho
JH
Kang
Mun-Il
MI
Cho
Kyoung-Oh
KO
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002417:Cattle / Q000821:virology*; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D016865:Reassortant Viruses / Q000235:genetics; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D013552:Swine / Q000382:microbiology; D014118:Toxins, Biological / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics
D000956:Antigens, Viral; D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; C082740:NSP3 protein, Rotavirus; D014118:Toxins, Biological; C060339:VP4 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins