Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability.
Surface wettability is an important physicochemical property of biomaterials, and it would be more helpful for understanding this property if a wide range of wettability are employed. This study focused on the effect of surface wettability on fibroblast adhesion over a wide range of wettability using a single material without changing surface topography. Plasma polymerization with hexamethyldisiloxane followed by oxygen (O2)-plasma treatment was employed to modify the surfaces. The water contact angle of sample surfaces varied from 106 degrees (hydrophobicity) to almost 0 degrees (super-hydrophilicity). O2 functional groups were introduced on polymer surfaces during O2-plasma treatment. The cell attachment study confirmed that the more hydrophilic the surface, the more fibroblasts adhered in the initial stage that includes super-hydrophilic surfaces. Cells spread much more widely on the hydrophilic surfaces than on the hydrophobic surfaces. There was no significant difference in fibroblast proliferation, but cell spreading was much greater on the hydrophilic surfaces. The fibronectin adsorbed much more on a hydrophilic surface while albumin dominated on a hydrophobic surface in a competing mode. These findings suggest the importance of the surface wettability of biomaterials on initial cell attachment and spreading. The degree of wettability should be taken into account when a new biomaterial is to be employed.
Wei
Jianhua
J
Department of Dental Materials Science and Oral Health Science Center, Tokyo Dental College, 1-2-2 Masago, Chiba 261-8502, Japan.
Yoshinari
Masao
M
Takemoto
Shinji
S
Hattori
Masayuki
M
Kawada
Eiji
E
Liu
Baolin
B
Oda
Yutaka
Y
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D005347:Fibroblasts / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D005353:Fibronectins / Q000378:metabolism; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010100:Oxygen / Q000737:chemistry; D044967:Serum / Q000737:chemistry; D012833:Siloxanes / Q000737:chemistry*; D013499:Surface Properties; D017155:Wettability
D001672:Biocompatible Materials; D005353:Fibronectins; D012833:Siloxanes; C015917:hexamethyldisiloxane; D010100:Oxygen