Distinct bactericidal activities of bovine lactoferrin peptides LFampin 268-284 and LFampin 265-284: Asp-Leu-Ile makes a difference.
Two lactoferrampin (LFampin) peptides derived from bovine lactoferrin were compared with respect to their bactericidal activities. LFampin 265-284 killed a set of Gram-positive bacteria that were resistant to LFampin 268-284. The presence of 265Asp-Leu-267Ile did not simply lead to an overall increased potency, since higher concentrations of LFampin 265-284 than LFampin 268-284 were needed to kill the Gram-negative bacteria that were tested. The Asp-Leu-Ile sequence enhances the propensity of LFampin to adopt an alpha-helix, as shown by circular dichroism spectroscopy. These results suggest that the helical conformation of the peptide is an important determinant of the susceptibility of Gram-positive bacteria.
van der Kraan
Marieke I A
MI
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Van der Boechorststraat 7, 1081 BT Amsterdam, Netherlands.
Nazmi
Kamran
K
van 't Hof
Wim
W
Amerongen
Arie V Nieuw
AV
Veerman
Enno C I
EC
Bolscher
Jan G M
JG
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002417:Cattle; D002942:Circular Dichroism; D007781:Lactoferrin / Q000737:chemistry / Q000378:metabolism*; D007782:Lactoglobulins / Q000737:chemistry / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D007782:Lactoglobulins; D010446:Peptide Fragments; D010455:Peptides; C502040:lactoferrampin; D007781:Lactoferrin