title: Analysis of human leukaemias and lymphomas using extensive immunophenotypes from an antibody microarray.
abstract: A novel antibody microarray has been developed that provides an extensive immunophenotype of leukaemia cells. The assay is a solid phase cell-capture technique in which 82 antigens are studied simultaneously. This paper presents the analysis of 733 patients with a variety of leukaemias and lymphomas from peripheral blood and bone marrow. Discriminant Function Analysis of the expression profiles from these 733 patients and 63 normal subjects were clustered and showed high levels of consistency with diagnoses obtained using conventional clinical and laboratory criteria. The overall levels of consensus for classification using the microarray compared with established criteria were 93.9% (495/527 patients) for peripheral blood and 97.6% (201/206 patients) for bone marrow aspirates, showing that the extensive phenotype alone was frequently able to classify the disease when the leukaemic clone was the dominant cell population present. Immunophenotypes for neoplastic cells were distinguishable from normal cells when the leukaemic cell count was at least 5 x 10(9) cells/l in peripheral blood, or 20% of cells obtained from bone marrow aspirates. This technique may be a useful adjunct to flow cytometry and other methods when an extensive phenotype of the leukaemia cell is desired for clinical trials, research and prognostic factor analysis.
authors.affiliation: Medsaic Pty Ltd, Suite 145, level1, National Innovation Centre, Australian Technology Park, Garden Street, Eveleigh, Australia.
authors.forename: Larissa
authors.initials: L
authors.lastname: Belov
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Mulligan
authors.forename: Nicole
authors.initials: N
authors.lastname: Barber
authors.forename: Adrian
authors.initials: A
authors.lastname: Woolfson
authors.forename: Mike
authors.initials: M
authors.lastname: Scott
authors.forename: Kerryn
authors.initials: K
authors.lastname: Stoner
authors.forename: Jeremy S
authors.initials: JS
authors.lastname: Chrisp
authors.forename: William A
authors.initials: WA
authors.lastname: Sewell
authors.forename: Kenneth F
authors.initials: KF
authors.lastname: Bradstock
authors.forename: Linda
authors.initials: L
authors.lastname: Bendall
authors.forename: Dana S
authors.initials: DS
authors.lastname: Pascovici
authors.forename: Mervyn
authors.initials: M
authors.lastname: Thomas
authors.forename: Wendy
authors.initials: W
authors.lastname: Erber
authors.forename: Pauline
authors.initials: P
authors.lastname: Huang
authors.forename: Mary
authors.initials: M
authors.lastname: Sartor
authors.forename: Graham A R
authors.initials: GA
authors.lastname: Young
authors.forename: James S
authors.initials: JS
authors.lastname: Wiley
authors.forename: Surender
authors.initials: S
authors.lastname: Juneja
authors.forename: William G
authors.initials: WG
authors.lastname: Wierda
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Green
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Keating
authors.forename: Richard I
authors.initials: RI
authors.lastname: Christopherson
mesh_terms: D000208:Acute Disease; D015703:Antigens, CD / Q000097:blood; D000951:Antigens, Neoplasm / Q000097:blood; D001853:Bone Marrow / Q000276:immunology; D003937:Diagnosis, Differential; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D007938:Leukemia / Q000145:classification / Q000175:diagnosis / Q000276:immunology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis / Q000276:immunology; D008223:Lymphoma / Q000145:classification / Q000175:diagnosis / Q000276:immunology*; D016393:Lymphoma, B-Cell / Q000145:classification / Q000175:diagnosis / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm