Role of adipose differentiation-related protein in lung surfactant production: a reassessment.
Based on data developed with the use of isolated lipid droplets from neonatal rat lung lipofibroblasts, we speculated previously that the droplet coat protein, adipose differentiation-related protein (ADFP), mediated the transfer of lipids into type 2 lung epithelial cells for the production of surfactant phospholipids. The present studies were designed to test the role of ADFP in this transfer with the use of ADFP-coated lipid droplets from CHO fibroblast cells and a cultured human lung epithelial cell line. We found no role for ADFP in the lipid transfer and conclude that a lipase associated with the lipid droplets hydrolyzes their core triacylglycerols, releasing fatty acids that are taken up by the epithelial cells.
Magra
Amy L
AL
Membrane Regulation Section, Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-8028, USA.
Mertz
Pamela S
PS
Torday
John S
JS
Londos
Constantine
C
D000818:Animals; D016466:CHO Cells; D045744:Cell Line, Tumor; D006224:Cricetinae; D004847:Epithelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008055:Lipids / Q000737:chemistry; D008168:Lung / Q000378:metabolism*; D008565:Membrane Proteins / Q000502:physiology*; D008856:Microscopy, Fluorescence; D000070780:Perilipin-2; D051381:Rats; D013501:Surface-Active Agents / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
D008055:Lipids; D008565:Membrane Proteins; C000602982:PLIN2 protein, human; D000070780:Perilipin-2; C000602984:Plin2 protein, rat; D013501:Surface-Active Agents; D014280:Triglycerides