Involvement of phospholipase D1 in collagen type I production of human dermal fibroblasts.
In the current study, the involvement of phospholipase D (PLD) in the regulation of collagen type I production was examined using human dermal fibroblasts. Procollagen I production in the cells overexpressing PLD1, but not PLD2, was found to be increased compared with those in the vector control cells. To investigate the role of PLD1, we examined the effect of knockdown of endogenous PLD1 by small interference RNA (siRNA) on collagen production. The reduction of expression levels of PLD1 by siRNA transfection was accompanied by diminution of procollagen biosynthesis and also ribosomal S6 kinase 1 (S6K1) phosphorylation. The activity of mammalian target of rapamycin (mTOR) is essential for phosphorylation of S6K1 and the treatment of dermal fibroblasts with rapamycin, a potent inhibitor of mTOR abolished procollagen I production. These results suggest that PLD1 plays a crucial role in collagen type I production through mTOR signaling in human dermal fibroblast.
Ohguchi
Kenji
K
Gifu International Institute of Biotechnology, 1-1 Naka-Fudogaoka, Kakamigahara, Gifu 504-0838, Japan. kohguchi@giib.or.jp
Banno
Yoshiko
Y
Akao
Yukihiro
Y
Nozawa
Yoshinori
Y
D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis*; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000201:enzymology* / Q000378:metabolism; D006801:Humans; D010739:Phospholipase D / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011494:Protein Kinases / Q000378:metabolism; D034622:RNA Interference; D058570:TOR Serine-Threonine Kinases
D024042:Collagen Type I; D011494:Protein Kinases; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; C105606:phospholipase D2; D010739:Phospholipase D; C106801:phospholipase D1