title: Bioanalysis and pharmacokinetics of chitosan ester in rabbit serum by HPLC with postcolumn fluorescence derivatization.
abstract: Interest in antiatherosclerotic activity of chitosan ester (PS916) with a new form of sulfate amino polysaccharide derived from marine chitin has necessitated the development of a sensitive and specific method to study its pharmacokinetics. A sensitive and reproducible high-performance liquid chromatography (HPLC) with postcolumn fluorescence derivatization method was developed and validated for the determination of PS916 in rabbit serum. Chromatography was carried out using a C8 reversed-phase column with an isocratic mobile phase consisting of methanol-water (20:80, v/v) at a flow rate of 0.2 ml/min. The derivatization procedure involved postcolumn reaction with guanidine hydrochloride in an alkaline medium at 110 degrees C. The fluorometric detector was operated at 250 nm (excitation) and 435 nm (emission). The assay was linear over the concentration range of 5-100 microg/ml. The lower limit of detection (LLOD) was found to be 1.0 microg/ml. The proposed method was successfully applied for a pharmacokinetic study of PS916 in rabbits.
authors.affiliation: Department of Pharmacology, Marine Drug and Food Institute, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Han
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Lv
authors.forename: Tingfu
authors.initials: T
authors.lastname: Jiang
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D048271:Chitosan / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005453:Fluorescence; D011817:Rabbits; D015203:Reproducibility of Results
chemical_list: D048271:Chitosan