Lactic acid recovery from cheese whey fermentation broth using combined ultrafiltration and nanofiltration membranes.
The separation of lactic acid from lactose in the ultrafiltration permeate of cheese whey broth was studied using a cross-flow nanofiltration membrane unit. Experiments to test lactic acid recovery were conducted at three levels of pressure (1.4, 2.1, and 2.8 MPa), two levels of initial lactic acid concentration (18.6 and 27 g/L), and two types of nanofiltration membranes (DS-5DK and DS-5HL). Higher pressure caused significantly higher permeate flux and higher lactose and lactic acid retention (p < 0.0001). Higher initial lactic acid concentrations also caused significantly higher permeate flux, but significantly lower lactose and lactic acid retention (p < 0.0001). The two tested membranes demonstrated significant differences on the permeate flux and lactose and lactic acid retention. Membrane DS-5DK was found to retain 100% of lactose at an initial lactic acid concentration of 18.6 g/L for all the tested pressures, and had a retention level of 99.5% of lactose at initial lactic acid concentration of 27 g/L when the pressure reached 2.8 MPa. For all the tests when lactose retention reached 99-100%, as much as 64% of the lactic acid could be recovered in the permeate.
Li
Yebo
Y
Bioenvironmental Engineering Program, Department of Natural Resources and Environmental Design, North Carolina A&T State University, 1601 East Market Street, Greensboro, NC 27411, USA. yli@ncat.edu
Shahbazi
Abolghasem
A
D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002611:Cheese / Q000382:microbiology*; D003470:Culture Media / Q000302:isolation & purification*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005285:Fermentation / Q000502:physiology; D019344:Lactic Acid / Q000302:isolation & purification* / Q000378:metabolism*; D008567:Membranes, Artificial; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D014462:Ultrafiltration / Q000295:instrumentation* / Q000379:methods
D003470:Culture Media; D008567:Membranes, Artificial; D019344:Lactic Acid