Evidence of frequent integration of non-T-DNA vector backbone sequences in transgenic strawberry plant.
We have studied the occurrence of the integration of non-T-DNA sequences in transgenic strawberry plants obtained through Agrobacterium inoculation. DNA from these plants was subjected to PCR amplification of the sequence of the gene trfA, which is located outside the T-DNA. The percentage of trfA-positive plants varied from 40% to 90%, with a mean of 65.7%. Backbone sequences were confirmed by Southern blot analysis.
Abdal-Aziz
Samia Abdallah
SA
Departamento de Biología Vegetal, Universidad de Málaga, 29071 Málaga, Spain.
Pliego-Alfaro
Fernando
F
Quesada
Miguel A
MA
Mercado
José A
JA
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D031985:Fragaria / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics*
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C036483:T-DNA; C067405:TrfA protein, E coli