A simple and rapid spectrophotometric method for the determination of nitrite by its decolorizing effect on peroxovanadate complex.
A rapid, simple, and most economical spectrophotometric method was proposed for the determination of nitrite in various water samples, soil samples, and roots of leguminous plants. The method is based on decolorizing effect of nitrite on complex formed between hydrogen peroxide and vanadate in acidic medium. The decolorization of that complex by nitrite was exploited to monitor the reaction spectrophotometrically at 470 nm. The method was optimized for effect of concentrations of ammonium metavanadate, hydrogen peroxide, various acids, concentrations of sulphuric acid, order of reagents addition and color stability. The color of the complex was found to be stable for about 2 days, and the stability constant of the complex was also calculated by modified Job's method. The linearity range of the calibration graph was over 6.67-66.7 microg ml(-1) of nitrite with molar absorptivity, 0.276 x 10(3) mol(-1) l cm(-1) and Sandell's sensitivity, 0.1667 microg cm(-2). The method was applied successfully for the determination of nitrite in soil samples, various wastewater samples and roots of leguminous plants.
Galil
Mansour S Abdul
MS
Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore 570 006, India.
Mahadevaiah
Kumar
M S Yogendra
MS
Nagendrappa
G
G
D007887:Fabaceae / Q000737:chemistry; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D009573:Nitrites / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D012680:Sensitivity and Specificity; D012977:Sodium Nitrite / Q000032:analysis; D012987:Soil / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods; D014638:Vanadates / Q000737:chemistry*; D014867:Water / Q000032:analysis
D007202:Indicators and Reagents; D009573:Nitrites; D012987:Soil; C069276:peroxovanadate; D014867:Water; D014638:Vanadates; D006861:Hydrogen Peroxide; C101036:ammonium metavanadate; D012977:Sodium Nitrite