RAPD analysis of jasmine rice-specific genomic structure.
Total genomic DNA was extracted from 29 samples of rice seed, including jasmine rice Oryza sativa L. subsp indica 'KDML105', 'KDML105'-derived varieties, nonaromatic Thailand rice, and japonica rice. Polymorphism in RAPD profiles was analyzed to explore the genomic structure specific to jasmine rice. The degree of band sharing was used to evaluate genetic distance between varieties and to construct a phylogenetic tree. RD15, CNTLR85033, and CNT87040 were found to be closest to 'KDML105', which was consistent with the true relation among them. Four RAPD fragments that cooperatively distinguished jasmine rice from others were cloned and sequenced. PCR amplification using pairs of primers designed specifically further confirmed the credibility of the RAPD result. Comparison through Genbank revealed that a 454 bp RAPD band was similar to the first intron of a putative Cf2/Cf5 disease resistance gene and a 1,107 bp RAPD band similar to a wall-associated kinase (wak) gene sequence.
Wu
Y J
YJ
Institute of Food Safety, Chinese Academy of Inspection and Quarantine, No. 3, Gaobeidian North Road, Chaoyang District, Beijing 100025, PR China.
Chen
Y
Y
Wang
J
J
Zhu
C X
CX
Xu
B L
BL
D003433:Crosses, Genetic; D017343:Genes, Plant; D014644:Genetic Variation; D018745:Genome, Plant; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*