Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis.
Monolithic materials were prepared in capillaries by in situ polymerization of acrylamide, glycidyl methacrylate, and N,N'-methylenebisacrylamide in the presence of 1,4-butanediol, dodecanol, and DMSO as porogens. With Ampholine attached to the surface of the porous monolith via epoxide groups, a monolithic-IPG (M-IPG) was formed and showed good mechanical and chemical stability. With such a column immobilized by Ampholine 3.5-10, IEF-MIX 3.6-9.3 was separated and good linearity was obtained. The CIEF behavior of M-IPG was evinced by comparing the current with that in the open tubular capillary. In addition, the protein mixtures excreted from lung cancer cells of rats were analyzed with such a new M-IPG column.
Zhu
Guijie
G
National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
Yuan
Huiming
H
Zhao
Peng
P
Zhang
Lihua
L
Liang
Zhen
Z
Zhang
Weibing
W
Zhang
Yukui
Y
D000180:Acrylic Resins / Q000737:chemistry*; D002072:Butylene Glycols / Q000737:chemistry*; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D007851:Dodecanol / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016062:Porosity; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D000099166:Polyacrylamides
D000180:Acrylic Resins; D002072:Butylene Glycols; D004121:Dimethyl Sulfoxide; D007851:Dodecanol; D011506:Proteins; D000099166:Polyacrylamides; C039681:1,4-butanediol