title: Preparation of mixed alginate elicitors with high activity for the efficient production of 5'-phosphodiesterase by Catharanthus roseus cells.
abstract: When various autoclaved microbial cells suspensions (exogenous elicitors) were added to Catharanthus roseus cell cultures, its growth was inhibited but 5'-phosphodiesterase (PDase) production was stimulated. The greatest effect was with autoclaved Alteromonas macleodii: the dry cell concentration decreased from 13 to 10.9 mg/ml while PDase production increased from 0.022 to 0.235 U/ml. A combination of A. macleodii (as exogenous elicitor) and 0.1%(w/v) alginate oligomers (AO: acting as both endogenous elicitor and scavenger of active oxygen species) minimized the cell growth inhibition but enhanced PDase production (0.474 U/ml) about 20 times higher than the control (no addition). The method for the preparation of mixed alginate elicitors with high activities containing exogenous elicitor (autoclaved A. macleodii), endogenous elicitor (AO), and trans-4,5-dihydroxy-2-cyclopenten-1-one was developed. The mixed alginate elicitors significantly promoted PDase production (2.67 U/ml) by C. roseus, and the productivity was increased 120-fold compared to the control without cell growth inhibition.
authors.affiliation: Institute of Life Sciences and Bioengineering, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan. aoyagi@sakura.cc.tsukuba.ac.jp
authors.forename: Hideki
authors.initials: H
authors.lastname: Aoyagi
authors.forename: Chiharu
authors.initials: C
authors.lastname: Akimoto-Tomiyama
authors.forename: Hideo
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000494:pharmacology*; D019149:Bioreactors; D030008:Catharanthus / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000494:pharmacology*; D005658:Fungi / Q000201:enzymology; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D043264:Phosphodiesterase I / Q000096:biosynthesis* / Q000235:genetics; D015854:Up-Regulation
chemical_list: D000464:Alginates; D017931:DNA Primers; D005656:Fungal Proteins; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D043264:Phosphodiesterase I