title: Collagenase-3 (MMP-13) expression by inflamed mucosa in inflammatory bowel disease.
abstract: OBJECTIVE
  To determine whether the expression of collagenase-3 (MMP-13) in biopsies from patients with inflammatory bowel disease is correlated with histological inflammation parameters.
  
  
  METHODS
  Fifty-nine patients with inflammatory bowel disease were included in the study. The control group comprised 20 patients free of inflammatory disease and ten patients with acute diverticulitis. MMP-13 expression was determined by immunohistochemical staining and the specimens were assigned a histological inflammation score.
  
  
  RESULTS
  It was found that 62.8% of patients with ulcerative colitis (UC) and 54.1% of patients with Crohn's disease (CD) showed MMP-13-positive immunostaining in biopsies from affected areas. MMP-13-positive staining was more intense in ulcerated colonic mucosa. A positive and significant correlation was found between MMP-13 expression and the histological inflammation scores in mucosal samples from patients with CD (r = 0.74, p < 0.0001) or UC (r = 0.62, p < 0.0001). However, no MMP-13-positive immunostaining was found in either the biopsy specimens of the control group or those biopsies taken from patients with UC or CD in microscopically confirmed non-affected areas of the colonic mucosa. Similarly, colonic mucosa samples of the 10 patients with acute diverticulitis did not show immunostaining for MMP-13.
  
  
  CONCLUSIONS
  Our findings demonstrating the absence of MMP-13 expression in non-inflamed colonic mucosa or in acute diverticulitis, as well as a positive correlation between elevated MMP-13 expression and histological criteria of inflammation in patients with inflammatory bowel diseases (CD and UC) suggest a role of the protease in the pathogenesis of these latter processes.
authors.affiliation: Instituto Universitario de Oncología del Principado de Asturias, Oviedo, Gijón-Spain. fjvizoso@wanadoo.es
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Vizoso
authors.forename: Luis O
authors.initials: LO
authors.lastname: González
authors.forename: Maria D
authors.initials: MD
authors.lastname: Corte
authors.forename: Maria G
authors.initials: MG
authors.lastname: Corte
authors.forename: Miguel
authors.initials: M
authors.lastname: Bongera
authors.forename: Aranzazu
authors.initials: A
authors.lastname: Martínez
authors.forename: Aranzazu
authors.initials: A
authors.lastname: Martín
authors.forename: Alejandro
authors.initials: A
authors.lastname: Andicoechea
authors.forename: Richard R R
authors.initials: RR
authors.lastname: Gava
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D016099:Endoscopy, Gastrointestinal; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000201:enzymology* / Q000473:pathology; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000473:pathology; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis*; D008875:Middle Aged; D011379:Prognosis; D012189:Retrospective Studies; D012720:Severity of Illness Index
chemical_list: D053509:Matrix Metalloproteinase 13