title: [Prevalence of the gene encoding the outer membrane lipoprotein LipL32 in leptospires of different taxons].
abstract: Primers flanking the fragment sized 677 bp have been constructed on the basis of nucleotide sequences of the gene encoding the outer membrane lipoprotein LipL32. PCR-analysis was used to reveal the prevalence of gene lipL32 among 73 Leptospiraceae family strains representing different genera and genomic species. The gene lipL32 appeared to be conservative across the pathogenic species. In contrast, it was not detected in the genome of nonpathogenic free-living leptospires. Thus the developed PCR test-system with primers LEP21/LEP22 may be efficiently used to differentiate these two distinct ecological groups of leptospires.
authors.forename: A P
authors.initials: AP
authors.lastname: Samsonova
authors.forename: E M
authors.initials: EM
authors.lastname: Petrov
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Aliapkina
authors.forename: M S
authors.initials: MS
authors.lastname: Zemskaia
authors.forename: O V
authors.initials: OV
authors.lastname: Savel'eva
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Anan'ina
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D017931:DNA Primers; D004197:Disease Reservoirs / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics*; D014943:Global Health; D006801:Humans; D016953:Leptospiraceae / Q000235:genetics* / Q000472:pathogenicity; D007922:Leptospirosis / Q000382:microbiology; D008074:Lipoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014774:Virulence / Q000235:genetics; D014871:Water Microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D017931:DNA Primers; C406589:LipL32 protein, Leptospira; D008074:Lipoproteins