title: [Isolation and characterization of a benzothiophene-desulfurizing bacterium].
abstract: A benzothiophene-desulfurizing strain was isolated and identified as Gordonia sp. C-6, which could degrade benzothiophene (BT) in a way analogous to the 4S pathway. It was found strain C-6 had the ability to grow in KT-BT medium whose sole source of sulfur was BT or its derivatives, whereas it couldn't grow well in a medium with DBT and its derivatives as the sole source of sulfur, the desulfurized product was identified as 2-(2'-hydroxyphenyl) ethan-1-al or its isomer benzofuran by GC-MS analysis. Desulfurization activity of strain C-6 was investigated. About 0.15mmol/L(50%) BT was desulfurized by strain C-6 after it was incubated in KT-BT medium at 30 degrees C for 48h, the residual BT has volatilized during aerobic fermentation. The quantitative detection of the benzothiophene-desulfurizing products was established with o-hydroxyphenylacetic acid as standard compound, the standard curve was fitted by Matlab.
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Li
authors.forename: Kyongho
authors.initials: K
authors.lastname: Lee
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Feng-lai
authors.initials: FL
authors.lastname: Liang
authors.forename: Ru-lin
authors.initials: RL
authors.lastname: Liu
mesh_terms: D033601:Gordonia Bacterium / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013455:Sulfur / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*
chemical_list: D012336:RNA, Ribosomal, 16S; D013876:Thiophenes; C088015:benzothiophene; D013455:Sulfur