title: Efficacy of a Listonella anguillarum (syn. Vibrio anguillarum) vaccine for juvenile sea bass Dicentrarchus labrax.
abstract: The efficacy of a commercial bivalent Listonella anguillarum (serotype 01 and 02) vaccine (MICROViB, Microtek International) was tested on prime- and booster-immersion vaccinated sea bass Dicentrarchus labrax juveniles. We carried out 2 challenge tests on the prime-vaccinated fish, 50 and 90 d after initial vaccination. A second group of fish received a booster vaccination 60 d after the prime vaccination, and were tested with a single challenge 30 d later. Relative percent survival (RPS) was 92 and 84% (both p < 0.01) among the prime-vaccinated fish on the first and second challenges, respectively. The RPS of the booster-vaccinated sea bass was 100% (p < 0.01). Antibody titres were tested only among 10 prime-vaccinated and 10 unvaccinated (control) sea bass, 60 d post-immunisation, and were found to rise to 1/32 in the vaccinated fish. Our results demonstrate that MICROViB immersion vaccine can effectively protect juvenile sea bass from L. anguillarum infection.
authors.affiliation: Laboratory of Ichthyology and Ichthyopathology, Aristotle University of Thessaloniki, PO Box 395, 54124 Thessaloniki, Greece. panangel@vet.auth.gr
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Angelidis
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Karagiannis
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Crump
mesh_terms: D000372:Agglutination Tests / Q000379:methods / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology / Q000592:standards; D001492:Bass / Q000276:immunology / Q000382:microbiology; D005393:Fish Diseases / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D007117:Immunization, Secondary / Q000662:veterinary; D016019:Survival Analysis; D013997:Time Factors; D014737:Vibrionaceae / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines