Frozen tissue biobanks. Tissue handling, cryopreservation, extraction, and use for proteomic analysis.
The potential and the limitations of protein analysis of tissue samples are surveyed. The complexity, concentration range and dynamics of the human proteome are reviewed, as is the effect of handling and cryopreservation. Protein extraction, solubilization, resolution and detection are discussed, in relation to the properties of the human proteome.
Ericsson
Christer
C
Department of Oncology - Pathology, Karolinska Institutet, CCK R8:05, Karolinska University Hospital, Solna, 171 76 Stockholm, Sweden. christer.ericsson@ki.se
Franzén
Bo
B
Nistér
Monica
M
D015925:Cryopreservation; D005821:Genetic Techniques; D006801:Humans; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D014015:Tissue Banks
D011506:Proteins; D020543:Proteome