Immunogenicity and protective efficacy of recombinant pseudorabies virus expressing the two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus.
Porcine reproductive and respiratory syndrome virus (PRRSV) infection still remains today as the most significant health threat to swine and poses a challenge to current vaccination strategies. To develop a new generation of vaccine against PRRSV, a live attenuated pseudorabies virus (PRV) was used as vaccine vector to express the two major membrane-associated proteins (GP5 or M) of PRRSV in various forms. Four PRV recombinants, rPRV-GP5 (expressing native GP5), rPRV-GP5m (expressing GP5m, a modified GP5), rPRV-GP5-M (co-expressing GP5 and M proteins), rPRV-GP5m-M (co-expressing GP5m and M proteins) were generated. Mouse immunized with all these recombinants developed comparable PRV-specific humoral immune responses and provided complete protection against a lethal PRV challenge. However, the highest level of PRRSV-specific neutralizing antibodies and lymphocyte proliferative responses was observed in mice immunized with rPRV-GP5m-M. The immunogenicity and protective efficiency of rPRV-GP5m-M were further evaluated in the piglets. Compared to commercial PRRSV killed vaccine, detectable PRRSV-specific neutralizing antibody and higher lymphocyte proliferative responses could be developed in piglets immunized with rPRV-GP5m-M before virus challenge. Furthermore, more efficient protection against a PRRSV challenge was obtained in piglets immunized with rPRV-GP5m-M, as showed by the balanced body-temperature fluctuation, shorter-term viremia, lower proportion of virus load in nasal and oropharyngeal scrapings and tissues, and milder lung lesions. These data indicate that the recombinant rPRV-GP5m-M is a promising candidate bivalent vaccine against both PRV and PRRSV infection.
Jiang
Yunbo
Y
Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
Fang
Liurong
L
Xiao
Shaobo
S
Zhang
Hui
H
Pan
Yongfei
Y
Luo
Rui
R
Li
Bin
B
Chen
Huanchun
H
D000818:Animals; D001831:Body Temperature / Q000502:physiology; D049109:Cell Proliferation; D005260:Female; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000276:immunology*; D011557:Pseudorabies / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D022421:Pseudorabies Vaccines / Q000276:immunology*; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins / Q000276:immunology; D014766:Viremia / Q000821:virology; D017201:Virus Shedding
D008565:Membrane Proteins; D022421:Pseudorabies Vaccines; D012367:RNA, Viral; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins