[Effect of conjugated linoleic acid on the gene expression of the fatty acid binding proteins in resistant rat].
OBJECTIVE
To study the effect of conjugated linoleic acid on gene expression of fatty acid binding proteins (ap2) in white obesity tissue of rats with insulin resistance, and explore the mechanism of resisting diabetes by CLA.


METHODS
Male Wistar rats were randomly separated to control group, high-fat group and high fat + CLA group (0.75g, 1.50g, 3.00g by diet weight), Using reverse transcription polymerse chain reaction (RT-PCR) technique to measure the expression level of ap2 and Peroxisome proliferators-activated receptor--gamma (PPARgamma) mRNA expression.


RESULTS
The FFA, serum insulin and glucose levels of rats with insulin resistance were higher than that of rats fed with basic diet, supplement of CLA can decrease FFA, serum insulin and glucose levels of rats with insulin resistance, and CLA can increase the expression of ap2 and PPARgamma mRNA in adipose tissue of rat with insulin resistance.


CONCLUSIONS
CLA can improve insulin resistance of obese rat and increase the expression of ap2 mRNA,possibly acting through activing PPARgamma.
Sun
Chang-Hao
CH
School of Public Health, Harbin Medical University, Harbin 150086, China.
Zhou
Xiao-Rong
XR
Zhao
Dan
D
D000818:Animals; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D007333:Insulin Resistance; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008297:Male; D009765:Obesity / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
C493407:Fabp2 protein, rat; D050556:Fatty Acid-Binding Proteins; D044243:Linoleic Acids, Conjugated; D047495:PPAR gamma; D012333:RNA, Messenger