Alternate-site isotopic labeling of ribonucleotides for NMR studies of ribose conformational dynamics in RNA.
Heteronuclear NMR spin relaxation studies of conformational dynamics are coming into increasing use to help understand the functions of ribozymes and other RNAs. Due to strong 13C-13C magnetic interactions within the ribose ring, however, these studies have thus far largely been limited to (13)C and (15)N resonances on the nucleotide base side chains. We report here the application of the alternate-site (13)C isotopic labeling scheme, pioneered by LeMaster for relaxation studies of amino acid side chains, to nucleic acid systems. We have used different strains of E. coli to prepare mononucleotides containing (13)C label in one of two patterns: Either C1' or C2' in addition to C4', termed (1'/2',4') labeling, or nearly complete labeling at the C2' and C4' sites only, termed (2',4') labeling. These patterns provide isolated 13C-1H spin systems on the labeled carbon atoms and thus allow spin relaxation studies without interference from 13C-13C scalar or dipolar coupling. Using relaxation studies of AMP dissolved in glycerol at varying temperature to produce systems with correlation times characteristic of different size RNAs, we demonstrate the removal of errors due to 13C-13C interaction in T (1) measurements of larger nucleic acids and in T (1rho) measurements in RNA molecules. By extending the applicability of spin relaxation measurements to backbone ribose groups, this technology should greatly improve the flexibility and completeness of NMR analyses of conformational dynamics in RNA.
Johnson
James E
JE
Department of Biochemistry & Molecular Biology, Michigan State University, 212 Biochemistry Building, East Lansing, MI, 48824, USA.
Julien
Kristine R
KR
Hoogstraten
Charles G
CG
D002247:Carbon Isotopes; D004926:Escherichia coli / Q000235:genetics; D005990:Glycerol / Q000737:chemistry; D007553:Isotope Labeling; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D012329:RNA, Bacterial / Q000737:chemistry; D012266:Ribose / Q000737:chemistry*; D012267:Ribosemonophosphates / Q000096:biosynthesis
D002247:Carbon Isotopes; D012329:RNA, Bacterial; D012267:Ribosemonophosphates; C031626:ribose-5-phosphate; D012313:RNA; D012266:Ribose; D005990:Glycerol