title: Backbone dynamics of a biologically active human FGF-1 monomer, complexed to a hexasaccharide heparin-analogue, by 15N NMR relaxation methods.
abstract: The binding site and backbone dynamics of a bioactive complex formed by the acidic fibroblast growth factor (FGF-1) and a specifically designed heparin hexasaccharide has been investigated by HSQC and relaxation NMR methods. The comparison of the relaxation data for the free and bound states has allowed showing that the complex is monomeric, and still induces mutagenesis, and that the protein backbone presents reduced motion in different timescale in its bound state, except in certain points that are involved in the interaction with the fibroblast growth factor receptor (FGFR).
authors.affiliation: Departamento de Estructura y Función de Proteínas, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu, 9, 28006, Madrid, Spain.
authors.forename: Angeles
authors.initials: A
authors.lastname: Canales-Mayordomo
authors.forename: Rosa
authors.initials: R
authors.lastname: Fayos
authors.forename: Jesús
authors.initials: J
authors.lastname: Angulo
authors.forename: Rafael
authors.initials: R
authors.lastname: Ojeda
authors.forename: Manuel
authors.initials: M
authors.lastname: Martín-Pastor
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Nieto
authors.forename: Manuel
authors.initials: M
authors.lastname: Martín-Lomas
authors.forename: Rosa
authors.initials: R
authors.lastname: Lozano
authors.forename: Guillermo
authors.initials: G
authors.lastname: Giménez-Gallego
authors.forename: Jesús
authors.initials: J
authors.lastname: Jiménez-Barbero
mesh_terms: D000595:Amino Acid Sequence; D016220:Fibroblast Growth Factor 1 / Q000737:chemistry*; D006493:Heparin / Q000031:analogs & derivatives* / Q000737:chemistry*; D006801:Humans; D008956:Models, Chemical; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009844:Oligosaccharides / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D009587:Nitrogen Isotopes; D009844:Oligosaccharides; D016220:Fibroblast Growth Factor 1; D006493:Heparin; D009584:Nitrogen