title: Changes in ornithine decarboxylase activity in response to temperature stress and stimulation of juvenile hormone in Anastrepha fraterculus (Diptera, Tephritidae).
abstract: Ornithine decarboxylase (ODC) activity was analyzed in Anastrepha fraterculus (Diptera) females (4 days old) submitted to temperature stress (6 degrees C and 20/6 degrees C) and the topical application of juvenile hormone (JH). ODC activity and ejaculatory apodeme measurements (length and width) were made in males (15 days old) after 6 degrees C stress. JH dose of 500 ng and incubation of 3, 7, and 18 h increased ODC activity. Females reared at 6 degrees C and 20/6 degrees C had higher ODC activity than those reared at 25 degrees C. The treatment of 6 degrees C and JH incubation for 1 h increased ODC activity when compared to 6 degrees C treatments only. However, the treatment of 20/6 degrees C only after 3 or 18 h of JH incubation resulted in higher ODC activity than controls (20/6 degrees C) or 20/6 degrees C plus 1 h of JH incubation. Males did not undergo differences in ODC activity when reared at 6 degrees C or 25 degrees C but the ejaculatory apodeme measurements was higher in those reared at 25 degrees C than in those reared at 6 degrees C. The results can be considered an adaptive process to environmental changes.
authors.affiliation: Departamento de Genética, Laboratório de Genética do Desenvolvimento, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. anastrefa@ig.com.br
authors.forename: V V
authors.initials: VV
authors.lastname: Cardoso
authors.forename: P R
authors.initials: PR
authors.lastname: Prestes
authors.forename: J C F
authors.initials: JC
authors.lastname: Moreira
authors.forename: A K
authors.initials: AK
authors.lastname: Oliveira
mesh_terms: D000287:Administration, Topical; D000704:Analysis of Variance; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005837:Genitalia, Male / Q000187:drug effects; D007605:Juvenile Hormones / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D013696:Temperature; D033621:Tephritidae / Q000187:drug effects / Q000201:enzymology*; D013997:Time Factors
chemical_list: D007605:Juvenile Hormones; D009955:Ornithine Decarboxylase