Agkistin-s, a disintegrin domain, inhibits angiogenesis and induces BAECs apoptosis.
Previous work in our laboratory has shown agkistin, a snake venom metalloproteases (SVMPs) from the venom of Agkistrodon halys, possesses antiplatelet aggregation activity. In this study, we further examined the antiangiogenic activity of agkistin-s, the disintegrin domain of agkistin. Recombinant agkistin-s was produced in Escherichia coli by subcloning its cDNA into pET28a vector, and the effect of purified agkistin-s was evaluated. At the concentration of 0.5-1.5 microM, the recombinant agkistin-s exhibited inhibitory activities on the bovine aortic endothelial cells (BAECs) migration and proliferation in a dose-dependent manner. In addition, it exhibited an effective antiangiogenic effect when assayed by using the 10-day-old embryo chick CAM model and effectively inhibits the tube-like structure formation. Furthermore, it potently induced BAECs apoptosis as examined by flow cytometric assays.
Ren
Aixia
A
Institute of Biochemistry and Biotechnology, College of Life Sciences, Zhejiang University, Hangzhou 310029, China.
Wang
Shaohui
S
Cai
Wenjie
W
Yang
Guanzhen
G
Zhu
Yichun
Y
Wu
Xiangfu
X
Zhang
Yaozhou
Y
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002417:Cattle; D002478:Cells, Cultured; D002645:Chickens; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D009389:Neovascularization, Pathologic; D011994:Recombinant Proteins / Q000494:pharmacology; D014757:Viper Venoms / Q000494:pharmacology*
D011994:Recombinant Proteins; D014757:Viper Venoms; C411880:agkistin