Biosynthesis of 2-deoxystreptamine-containing antibiotics in Streptoalloteichus hindustanus JCM 3268: characterization of 2-deoxy-scyllo-inosose synthase.
A part of the new biosynthetic gene cluster for 2-deoxystreptamine-containing antibiotics was identified from Streptoalloteichus hindustanus. The alloH gene in the gene cluster was deduced to encode 2-deoxy-scyllo-inosose synthase and the expressed protein AlloH was confirmed to have this enzyme activity. Furthermore, biochemical properties of AlloH were studied.
Hirayama
Toshifumi
T
Department of Chemistry and Materials Science, Tokyo Institute of Technology, Japan.
Tamegai
Hideyuki
H
Kudo
Fumitaka
F
Kojima
Kazumasa
K
Kakinuma
Katsumi
K
Eguchi
Tadashi
T
D000192:Actinomycetales / Q000235:genetics / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D006595:Hexosamines / Q000096:biosynthesis; D008190:Lyases / Q000235:genetics*; D005810:Multigene Family
D000900:Anti-Bacterial Agents; D006595:Hexosamines; D008190:Lyases; C085631:2-deoxy-scyllo-inosose synthase; C007265:2-deoxystreptamine