title: Regionally distinct patterns of calpain activation and traumatic axonal injury following contusive brain injury in immature rats.
abstract: Impact-induced head injury in infants results in acute focal contusions and traumatic axonal injury (TAI) that are associated with chronic holohemispheric cortical and white matter atrophy and may contribute to poor outcome in brain-injured children less than 4 years of age. Contusive brain trauma in postnatal day (PND) 11 or PND 17 rat pups, ages neurologically equivalent to a human infant and toddler, respectively, leads to cortical tissue loss and white matter atrophy which are associated with cognitive deficits. In adult models of brain trauma and in brain-injured humans, acute and sustained activation of the calpain family of calcium-activated neutral proteases has been implicated in neuronal death and TAI. PND 11 or PND 17 rat pups were subjected to closed head injury over the left hemisphere using the controlled cortical impact device and sacrificed at 6 h, 24 h or 3 days. Hemorrhagic contusions and tissue tears in the cortex and white matter were visible at 6 h, and neuronal loss was evident by 3 days. Calpain activation was observed in cell soma and dendrites of injured neurons at 6 h, and in degenerating dendrites and atrophic neurons at 24 h after injury at both ages. Axonal accumulation of amyloid precursor protein, indicative of TAI, was observed in the corpus callosum and lateral aspects of the white matter below the site of impact, and in the thalamus in PND 11 rats only. Intra-axonal calpain activation was observed to a limited extent in the corpus callosum and subcortical white matter tracts in both brain-injured PND 11 and PND 17 rats. Collectively, these results provide evidence that calpain activation may participate in neuronal loss in the injured cortex, but may not contribute to the pathogenesis of TAI following contusive brain trauma in the immature rat.
authors.affiliation: Department of Anesthesiology and Critical Care, Children's Hospital of Philadelphia, and Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129, USA.
authors.forename: Jimmy W
authors.initials: JW
authors.lastname: Huh
authors.forename: Michael A
authors.initials: MA
authors.lastname: Franklin
authors.forename: Ashley G
authors.initials: AG
authors.lastname: Widing
authors.forename: Ramesh
authors.initials: R
authors.lastname: Raghupathi
mesh_terms: D000375:Aging / Q000502:physiology; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers; D001921:Brain / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D001930:Brain Injuries / Q000378:metabolism* / Q000503:physiopathology*; D002154:Calpain / Q000378:metabolism*; D016923:Cell Death / Q000502:physiology; D002544:Cerebral Infarction / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D003712:Dendrites / Q000378:metabolism / Q000473:pathology; D020833:Diffuse Axonal Injury / Q000209:etiology / Q000503:physiopathology*; D004195:Disease Models, Animal; D009410:Nerve Degeneration / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D015854:Up-Regulation / Q000502:physiology
chemical_list: D016564:Amyloid beta-Protein Precursor; D015415:Biomarkers; D002154:Calpain