The reversible binding of anti-human serum albumin to poly beta-cyclodextrin-coated porous silica supports.
A supramolecular system involving host-guest interactions between immobilized beta-cyclodextrin (beta-CD) cavities and adamantyl groups was evaluated for the preparation of immunosorbents which can be regenerated after use. First a dextran layer bearing both adamantyl groups and carboxylic functions is immobilized onto beta-CD-modified porous silica particles (400 nm) by formation of inclusion complexes. Then, antibody molecules are grafted to the polymer layer. The stationary phases can be prepared in batch or directly in the column. They are stable in aqueous media and are able to trap specifically the corresponding antigen. In case of alteration of the antibody layer, it is possible to remove it by passing a SDS solution through the column. The feasibility of the procedure was evaluated, using the anti-HSA/HSA system.
Karakasyan
C
C
Laboratoire de Recherche sur les Polymères, CNRS-Université Paris 12 Val de Marne, UMR 7581, 2-8 rue Henri Dunant, F-94320 Thiais, France.
Sébille
B
B
Millot
M C
MC
D000218:Adamantane / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003911:Dextrans / Q000737:chemistry; D006801:Humans; D007120:Immunochemistry; D007164:Immunosorbents / Q000138:chemical synthesis; D012709:Serum Albumin / Q000276:immunology / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000906:Antibodies; D003911:Dextrans; D007164:Immunosorbents; D012709:Serum Albumin; D047392:beta-Cyclodextrins; D012822:Silicon Dioxide; C031215:betadex; D000218:Adamantane