Microbiological assay for lomefloxacin in coated tablets.
The validation of a microbiological assay, applying cylinder plate method for determination of the activity of lomefloxacin in coated tablets is described. Using a strain of Bacillus subtilis ATCC 9372 as the test organism, lomefloxacin was measured in concentrations ranging from 2.0 to 8.0 microg/mL. The method validation showed that it is linear (r = 0.9999), precise (relative standard deviation = 1.15%), and accurate (it measured the added quantities). The excipients did not interfere in the determination. It was concluded that the microbiological assay is satisfactory for quantitation of lomeflox tablets.
Gomes
Greici Cristiani
GC
Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, UNESP, Rod. Araraquara-Jaú, km 1, CEP 14801-902, Araraquara, SP, Brazil.
Salgado
Hérida Regina Nunes
HR
D000995:Antitubercular Agents / Q000032:analysis*; D001412:Bacillus subtilis / Q000378:metabolism; D001681:Biological Assay; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004058:Diffusion; D024841:Fluoroquinolones / Q000032:analysis*; D008826:Microbial Sensitivity Tests; D008828:Microbiological Techniques / Q000379:methods*; D008956:Models, Chemical; D015363:Quinolones / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets
D000995:Antitubercular Agents; D024841:Fluoroquinolones; D015363:Quinolones; D013607:Tablets; C053091:lomefloxacin