[Labeling of CDTPA-dianhydride-coupled CD45 monoclonal antibody with yttrium-90].
OBJECTIVE
To explore the methods for labeling CDTPA-coupled CD45 monoclonal antibody (mAb) with yttrium-90 ((90)Y) for potential acute myeloid therapy.


METHODS
CD45 mAb was labeled with (90)Y by CDTPA and the labeling rate, radiochemical purity, final specific activity, and immunological activity of the mAb were detected.


RESULTS
With the optimal molar ratio of CDTPA/Ab at 20:1, the labeling rate was 95%, radiochemical purity 99.8%, and final specific activity 1.9 mCi/mg. This conjugate was stable in vitro with comparable immunological activity in comparison with unlabeled CD45 mAb.


CONCLUSIONS
(90)Y-CDTPA-CD45 mAb possesses good properties as an ideal targetting therapeutic agent for acute leukemia.
Fu
Yun-bi
YB
Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. hnfyb@yahoo.com.cn
Li
Gui-ping
GP
Meng
Fan-yi
FY
D000812:Anhydrides / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry* / Q000276:immunology; D007553:Isotope Labeling / Q000379:methods*; D017493:Leukocyte Common Antigens / Q000276:immunology*; D004369:Pentetic Acid / Q000737:chemistry; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D015021:Yttrium Radioisotopes / Q000737:chemistry
D000812:Anhydrides; D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D019275:Radiopharmaceuticals; D015021:Yttrium Radioisotopes; D004369:Pentetic Acid; D017493:Leukocyte Common Antigens