[Effects of D-limonene on leukemia cells HL-60 and K562 in vitro].
To investigate the effects of D-limonene (D-L) on the cell growth and apoptosis in HL-60, K562 cells and to elucidate its mechanism, the influence of D-L on proliferation of HL-60 and K562 cells was determined by propidium iodide assay, the expression levels of mutant p53, bcl-2, bax gene were detected by cell morphological analysis, flow cytometry and immunohistochemistry staining, the D-L-inducing HL-60 and K562 cell apoptosis in vitro was observed systematically. The results showed that D-L inhibited HL-60 and K562 cell growth in a dose- and time-dependent manner with the IC50 of 0.75 mmol/L similarly, D-L induced apoptosis of HL-60 and K562 cells, and expression of bcl-2 gene was down regulated by D-L in a concentration-dependent manner in HL-60 cells. The bcl-2, mutant type of p53 genes were down regulated while bax gene was up regulated by D-L in a concentration-dependent manner in K562 cells. It is concluded that D-L can inhibit proliferation and induce apoptosis of HL-60 and K562 cells. The bcl-2, mutant type of p53 and bax may be involved in the gene regulation of D-L-induced apoptosis.
Guo
Xiao-Ming
XM
Department of Pediatrics, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China.
Lu
Qing
Q
Liu
Zheng-Juan
ZJ
Wang
Li-Feng
LF
Feng
Bing-An
BA
D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D053138:Cyclohexenes / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D000077222:Limonene; D009154:Mutation; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D013729:Terpenes / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D051028:bcl-2-Associated X Protein / Q000235:genetics
D053138:Cyclohexenes; D019253:Proto-Oncogene Proteins c-bcl-2; D013729:Terpenes; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D000077222:Limonene