Biologically active endophytic streptomycetes from Nothofagus spp. and other plants in Patagonia.
Endophytic streptomycetes have been isolated and characterized from several species of Nothofagus and other plants growing in the southern reaches of Patagonia. No endophytic streptomycete was obtained from any plant species studied in Northern Patagonia. However, from Southern Patagonia, biologically active Streptomyces spp. from several plant species were isolated. Each isolate, as studied by scanning electron microscopy (SEM), has small hyphae, some produce typical barrel-shaped spores in culture and each has some unique hyphal surface structures. Interestingly, although none has any detectable antibacterial killing properties, each has demonstrable killing activity against one or more pathogenic fungi including representative plant pathogenic organisms such as Phytophthora erythroseptica, Pythium ultimum, Sclerotinia sclerotiorum, Mycosphaerella fijiensis, and Rhizoctonia solani. The 16S rDNA sequences of the isolates were distinct from all other genetic accessions of Streptomyces in GenBank. However, isolate C-2 from Chiliotrichum diffusum (Compositae) is identical, in all respects, to isolate C-4 obtained from Misodendrum punctulatum (Loranthaceae). These results confirm that endophytic streptomycetes represent a novel source of biologically active microorganisms.
Castillo
Uvidello F
UF
Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA.
Browne
Lindsey
L
Strobel
Gary
G
Hess
W M
WM
Ezra
Sigal
S
Pacheco
Gladys
G
Ezra
David
D
D000898:Antibiosis; D019659:Asteraceae / Q000382:microbiology; D029962:Fagaceae / Q000382:microbiology*; D005658:Fungi / Q000254:growth & development*; D028183:Loranthaceae / Q000382:microbiology; D019684:Magnoliopsida / Q000382:microbiology*; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009868:Oomycetes / Q000254:growth & development*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013020:South America; D013302:Streptomyces / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure