[Effect of HuperzineA on neurophathologic change of mouse exposed by isocarbophos and phoxim].
OBJECTIVE
In order to assessment the Huperzine A (HupA) on CNS of mice exposed by phoxim and isocarbophos.


METHODS
One group was given tween-80 or peanut oil as control. One group was given phoxim or isocarbophos alone as phoxim or isocarbophos group. The other group was given HupA 2h before phoxim or isocarbophos exposure as HupA protective group. Twenty-four hours after exposure, the whole body perfusion in situ technique was performed for tissue fixation. The whole brain was removed and adjacent coronal sections was obtained. The sections of brain were stained with hematoxylin and eosin (HE) and toluidine blue. Under optic microscope images were collected and analysed.


RESULTS
The extensive lesions including cortex oedema, pyramidal cell oedema, and nissal body numbers reduced were observed in phoxim or isocarbophos group. The animals of HupA protective group were observed cortex oedema, pyramidal cell oedema, but the lesional degree was lighter obviously than phoxim or isocarbophos group.


CONCLUSIONS
It was suggested that the HupA could have the protective effect of neuropathologic changes exposed by phoxim and isocarbophos.
Xie
Guang-Yun
GY
National Institute of Occupational Health and Poisons Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Liu
Li
L
Wang
Jian
J
Zhao
Wen-Jin
WJ
D000470:Alkaloids; D000818:Animals; D001921:Brain / Q000473:pathology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D007306:Insecticides / Q000633:toxicity; D008294:Malathion / Q000031:analogs & derivatives* / Q000633:toxicity; D008297:Male; D051379:Mice; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D011897:Random Allocation; D012717:Sesquiterpenes / Q000494:pharmacology*
D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D007306:Insecticides; D018696:Neuroprotective Agents; D009946:Organothiophosphorus Compounds; D012717:Sesquiterpenes; C050426:huperzine A; C003135:phoxim; C549713:isocarbophos; D008294:Malathion