Hypercholesterolemia and LDL receptor mRNA expression: modulation by selenium supplementation.
Selenium (Se) status has been associated with cardiovascular disorders. Present study was aimed to elucidate the protective role of Se supplementation on LDL receptor (LDL-R) activity as well as mRNA expression during experimental hypercholesterolemia in SD male rats. Animals were fed 0.2 and 1 ppm Se supplemented control diet as well as 2% cholesterol supplemented diet for 3 months. LDL-R activity was measured in-vivo by injecting radiolabeled LDL to rats and decrease in counts per minute with time was taken as a measure of LDL clearance and in turn LDL-R activity. LDL-R mRNA expression was studied by RT-PCR. LDL-R activity and mRNA expression decreased significantly on 2% cholesterol supplemented diet feeding. On 1 ppm Se supplementation LDL-R activity as well as mRNA expression increased significantly. Present results demonstrate that Se supplementation upto 1 ppm is responsible for up regulation of LDL-R activity as well as mRNA expression, during hypercholesterolemia. These findings highlight the therapeutic potential of Se supplementation in lipid metabolism.
Dhingra
Sanjiv
S
Department of Biophysics, Punjab University, Chandigarh, 160014, India.
Bansal
Mohinder P
MP
D000818:Animals; D002784:Cholesterol / Q000097:blood; D019587:Dietary Supplements; D005979:Glutathione Peroxidase / Q000378:metabolism; D006937:Hypercholesterolemia / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism*; D012643:Selenium / Q000008:administration & dosage*; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
D008077:Lipoproteins, LDL; D012333:RNA, Messenger; D011973:Receptors, LDL; D014284:Triiodothyronine; D002784:Cholesterol; D005979:Glutathione Peroxidase; D012643:Selenium; D013974:Thyroxine