Functional activity of mitochondria in cultured neural precursor cells.
We studied mitochondrial transmembrane potential of neural precursor cells forming neurospheres in culture. Uneven energization of mitochondria in neurosphere cells was detected. Heterogeneity of cells by the mitochondrial potential increased with neurosphere enlargement during culturing. Decrease in the mitochondrial potential in the central cells in large spheres, presumably caused by insufficient diffusion of oxygen and nutrients, can provoke their damage and death. Population of cells with high mitochondrial potential responded to addition of the nuclear dye by a decrease in mitochondrial potential, which can indicate functioning of ABCG2 complex in these cells, characteristic of undifferentiated stem cells. These data will help to create optimum conditions for culturing of neural stem cells for the maintenance of their maximum functional and proliferative activity.
Plotnikov
E Yu
EY
A N Belozvorskii Institute of Physico-Chemical Biology, M V Lomonosov Moscow State University. plotnikov@libro.genebee.msu.ru
Marei
M V
MV
Podgornyi
O V
OV
Aleksandrova
M A
MA
Zorov
D B
DB
Sukhikh
G T
GT
D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000032:analysis; D001562:Benzimidazoles / Q000494:pharmacology; D002232:Carbocyanines / Q000494:pharmacology; D018929:Cell Culture Techniques; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000502:physiology*; D009363:Neoplasm Proteins / Q000032:analysis; D009474:Neurons / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D009942:Organometallic Compounds / Q000494:pharmacology; D013234:Stem Cells / Q000502:physiology* / Q000648:ultrastructure
C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D001562:Benzimidazoles; D002232:Carbocyanines; D009363:Neoplasm Proteins; D009942:Organometallic Compounds; C110932:tetramethyl rhodamine ethyl ester; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine