CD103 is a marker for alloantigen-induced regulatory CD8+ T cells.
The alphaEbeta7 integrin CD103 may direct lymphocytes to its ligand E-cadherin. CD103 is expressed on T cells in lung and gut and on allograft-infiltrating T cells. Moreover, recent studies have documented expression of CD103 on CD4+ regulatory T cells. Approximately 4% of circulating CD8+ T cells bear the CD103 molecule. In this study, we show that the absence or presence of CD103 was a stable trait when purified CD103- and CD103+ CD8+ T cell subsets were stimulated with a combination of CD3 and CD28 mAbs. In contrast, allostimulation induced CD103 expression on approximately 25% of purified CD103- CD8+ T cells. Expression of CD103 on alloreactive cells was found to be augmented by IL-4, IL-10, or TGF-beta and decreased by addition of IL-12 to MLCs. The alloantigen-induced CD103+ CD8+ T cell population appeared to be polyclonal and retained CD103 expression after restimulation. Markedly, in vitro-expanded CD103+ CD8+ T cells had low proliferative and cytotoxic capacity, yet produced considerable amounts of IL-10. Strikingly, they potently suppressed T cell proliferation in MLC via a cell-cell contact-dependent mechanism. Thus, human alloantigen-induced CD103+ CD8+ T cells possess functional features of regulatory T cells.
Uss
Elena
E
Department of Experimental Immunology, Academic Medical Center, Amsterdam, The Netherlands. a.uss@amc.uva.nl
Rowshani
Ajda T
AT
Hooibrink
Berend
B
Lardy
Neubury M
NM
van Lier
René A W
RA
ten Berge
Ineke J M
IJ
D015703:Antigens, CD / Q000276:immunology* / Q000378:metabolism*; D015415:Biomarkers; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D002450:Cell Communication / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation; D006801:Humans; D039001:Integrin alpha Chains / Q000276:immunology* / Q000378:metabolism*; D007378:Interleukins / Q000494:pharmacology; D007519:Isoantigens / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D015703:Antigens, CD; D015415:Biomarkers; D039001:Integrin alpha Chains; D007378:Interleukins; D007519:Isoantigens; D016212:Transforming Growth Factor beta; C091367:alpha E integrins