[Impaired microfilament cytoskeletal rearrangement in cytomegalovirus infected cells].
The objective of this study was to investigate the effect of cytomegalovirus (CMV) infection on actin and microfilament in human embryo fibroblast cells (HF) and to explore the possible relationship with CMV replication. The cell shape was observed by microscopy after the infection of CMV, RT-PCR assay was used to detect the mRNA expression of beta-actin gene, while Westen-blot was used to measure the level of beta-actin protein. CMV immediately early antigen (IE) in HF cells was analyzed by indirect immunofluorescence assay. Microfilament alteration was determined by cytoskeleton fluorescence probe. The results showed that CMV IE was observed in more than 95% of HF cells after infection, primarily located in nucleus. HF cells infected by CMV changed from thin shuttle shape to round and thick ball shape, even detached from wall. Beta-actin got a significant and gradual decreasing of mRNA level in time-dependent manner (P < 0.05). Compared with uninfected group, the expression of beta-actin protein decreased to (74.2 +/- 13.4)% at 96 hours after infection (P < 0.05). In infected HF cells, microfilaments were ruptured, arranged turbulently, as well as cells merged and fluorescence density of microfilament obviously reduced. It is concluded that cytomegalovirus can induce alteration of actin and microfilament, which may be helpful for CMV to infect, replicate and reactivate in host cells.
Zhao
Guang-Sheng
GS
Department of Hematology, Bone Marrow Transplantation Center, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310003, China.
Lin
Mao-Fang
MF
D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure*; D000199:Actins / Q000378:metabolism; D002460:Cell Line; D003586:Cytomegalovirus Infections / Q000378:metabolism / Q000473:pathology*; D005347:Fibroblasts / Q000473:pathology / Q000648:ultrastructure / Q000821:virology*; D006801:Humans
D000199:Actins