title: Regenerative capacity of individual liver lobes in the microsurgical mouse model.
abstract: In the development of microsurgical mouse models of hepatic regeneration and repair, lobe-specific regenerative responses were observed. We therefore determined the hepatic regenerative capacity of individual mouse liver lobes. In mice, 26, 60, 75, and 83% of total liver mass was resected. Bromo-deoxyuridine (BrdU) was injected prior to liver harvest and the BrdU labeling index determined in all remaining individual liver lobes. BrdU-positive nuclei were seen in all liver lobes after the 26 and 60% resection, but significantly fewer were detected in the caudate lobe. In the 75% group, equally distributed positive nuclei were found. However, BrdU labeling was scant in the 83% group. In microsurgical mouse liver-regeneration models, the average hepatic response depends on amount of liver tissue resected and on the remaining liver lobe. BrdU incorporation can vary significantly among individual lobes. The lobe-specific differences observed may prove valuable in further investigations of hepatic regeneration and repair.
authors.affiliation: Department of Visceral and Transplantation Surgery, University Hospital Bern, Bern, Switzerland. daniel.inderbitzin@insel.ch
authors.forename: Daniel
authors.initials: D
authors.lastname: Inderbitzin
authors.forename: Peter
authors.initials: P
authors.lastname: Studer
authors.forename: Daniel
authors.initials: D
authors.lastname: Sidler
authors.forename: Guido
authors.initials: G
authors.lastname: Beldi
authors.forename: Valentin
authors.initials: V
authors.lastname: Djonov
authors.forename: Adrian
authors.initials: A
authors.lastname: Keogh
authors.forename: Daniel
authors.initials: D
authors.lastname: Candinas
mesh_terms: D000818:Animals; D005500:Follow-Up Studies; D006498:Hepatectomy / Q000379:methods*; D008099:Liver / Q000502:physiology / Q000601:surgery / Q000648:ultrastructure; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D008866:Microsurgery / Q000379:methods*; D009929:Organ Size / Q000502:physiology