Viability of Trichomonas vaginalis in urine: epidemiologic and clinical implications.
The impact of the viability of Trichomonas vaginalis in urine on wet mount, culture, and PCR methods was assessed. To minimize the chance of false-negative results, urine specimens should be processed within 30 min of specimen collection and maintained at 37 degrees C, since temperature appears to affect the viability of Trichomonas.
Shafir
Shira C
SC
UCLA School of Public Health, Department of Epidemiology, Center for Health Sciences, Room 41-275, 650 Charles E. Young Drive, Los Angeles, CA 90024, USA. sshafir@ucla.edu
Sorvillo
Frank J
FJ
D000818:Animals; D005188:False Negative Reactions; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D014245:Trichomonas Infections / Q000175:diagnosis* / Q000652:urine*; D014246:Trichomonas vaginalis / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*