title: Adiponectin-induced secretion of interleukin-6 (IL-6), monocyte chemotactic protein-1 (MCP-1, CCL2) and interleukin-8 (IL-8, CXCL8) is impaired in monocytes from patients with type I diabetes.
abstract: BACKGROUND
  Systemic adiponectin is reduced in patients with cardiovascular disease (CVD) and low adiponectin may contribute to the pathogenesis of atherosclerosis. However, circulating adiponectin is elevated in type 1 diabetes (T1D) patients, who have also a higher incidence to develop CVD. Because monocytes play an important role in atherosclerosis, we analysed the influence of adiponectin on cytokine and chemokine release in monocytes from T1D patients and controls.
  
  
  METHODS
  Systemic adiponectin was determined in the plasma and the high-molecular weight (HMW) form of adiponectin was analysed by immunoblot. Monocytes were isolated from T1D patients and controls and the adiponectin-stimulated release of interleukin-6 (IL-6), monocyte chemotactic protein-1 (MCP-1, CCL2) and interleukin-8 (IL-8, CXCL8) was analysed.
  
  
  RESULTS
  Systemic adiponectin was higher in T1D patients. Immunoblot analysis of the plasma indicate abundance of HMW adiponectin in T1D patients and controls. IL-6, CCL2 and CXCL8 secretion in response to adiponectin were found induced in monocytes from controls whereas only IL-6 was upregulated in T1D cells. The induction of IL-6 by adiponectin was abrogated by an inhibitor of the NFkappaB pathway.
  
  
  CONCLUSIONS
  These data indicate that adiponectin-mediated induction of IL-6, CCL2 and CXCL8 is disturbed in monocytes from T1D patients and therefore elevated systemic adiponectin in T1D patients may be less protective when compared to controls.
authors.affiliation: Department of Internal Medicine I, University of Regensburg, D-93042 Regensburg, Germany. abs507310@gw.ngate.uni-regensburg.de
authors.forename: Sabine
authors.initials: S
authors.lastname: Abke
authors.forename: Markus
authors.initials: M
authors.lastname: Neumeier
authors.forename: Johanna
authors.initials: J
authors.lastname: Weigert
authors.forename: Gabriele
authors.initials: G
authors.lastname: Wehrwein
authors.forename: Elke
authors.initials: E
authors.lastname: Eggenhofer
authors.forename: Andreas
authors.initials: A
authors.lastname: Schäffler
authors.forename: Kevin
authors.initials: K
authors.lastname: Maier
authors.forename: Charalampos
authors.initials: C
authors.lastname: Aslanidis
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schölmerich
authors.forename: Christa
authors.initials: C
authors.lastname: Buechler
mesh_terms: D052242:Adiponectin / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D015992:Body Mass Index; D018932:Chemokine CCL2 / Q000097:blood* / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood* / Q000378:metabolism; D016209:Interleukin-8 / Q000097:blood* / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D012016:Reference Values
chemical_list: D052242:Adiponectin; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D015850:Interleukin-6; D016209:Interleukin-8; D011994:Recombinant Proteins