[Evaluation of locomotor activity after a local induction of toxic demyelination in the brainstem of Wistar rats].
Ethidium-bromide (EB)-induced lesions have been used to investigate the incomplete remyelination in the central nervous system, as well as to evaluate therapeutic strategies to accelerate the reconstruction of the lost myelin sheaths. Although many electrophysiologic studies were performed in situations of experimental demyelination and remyelination, their behavioural effects have not been properly analyzed. In this study, we investigated ultrastructurally the EB - demyelinating lesions as well as the locomotor activity of rats during the beam walking test after a focal induction of demyelination using the EB model in the ventral surface of the brainstem. It was observed the occurrence of locomotor deficits until 31 days post-injection, as well as that subsequent remyelination was related to the return of the lost function.
Bondan
Eduardo Fernandes
EF
Universidade Paulista, Rua Caconde 125/51, 01425-011 São Paulo SP, Brazil. bondan@uol.com.br
Lallo
Maria Anete
MA
Orsini
Heloísa
H
Bentubo
Henri Levi Donnaruma
HL
Yazbek
Angela
A
Macrini
Daclê Juliani
DJ
Bernardi
Maria Martha
MM
Graça
Dominguita Luhers
DL
D000818:Animals; D001933:Brain Stem / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D003711:Demyelinating Diseases / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D004996:Ethidium; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D009186:Myelin Sheath / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D004996:Ethidium