title: [Screening of proteins interacting with hepatitis C virus NS3 from T7-phage display library].
abstract: OBJECTIVE
  To screen and identify proteins that interact with hepatitis C virus NS3 by means of T7-phage display system.
  
  
  METHODS
  Hepatitis C virus NS3 was expressed by prokaryotic expression and used as a selected molecule to biopan the T7 select human liver cDNA library; the selected positive clones were identified using DNA sequencing and analyzed with BLAST program in GenBank.
  
  
  RESULTS
  After BLAST analysis in all the positive clones, the proteins which interacted with the hepatitis C virus NS3 were found to be serpin peptidase inhibitor, clade A, member 1 (SERPINA1) and cyclophilin-LC.
  
  
  CONCLUSIONS
  T7-phage display system is a convenient, rapid and effective method for screening interacting proteins. The proteins thus selected will provide an important means for studying the pathogenesis and carcinogenesis of HCV.
authors.affiliation: Molecular Biology Laboratory of Infectious Diseases, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Xue-fei
authors.initials: XF
authors.lastname: Cai
authors.forename: Mao-rui
authors.initials: MR
authors.lastname: He
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Ai-long
authors.initials: AL
authors.lastname: Huang
mesh_terms: D002460:Cell Line; D015723:Gene Library; D016174:Hepacivirus / Q000378:metabolism*; D006801:Humans; D019151:Peptide Library; D025941:Protein Interaction Mapping / Q000379:methods*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D019151:Peptide Library; D014760:Viral Fusion Proteins; D017361:Viral Nonstructural Proteins