Novel membranes and surface modification able to activate specific cellular responses.
The design of new polymeric biomaterials together with new strategies to modify membrane surface are crucial to optimise cell-biomaterial interactions in vivo and in vitro biohybrid systems. In this study we report on the novel semipermeable membranes synthesised from a polymeric blend of modified polyetheretherketone and polyurethane able to support the long-term maintenance and differentiation of human liver cells and on the surface modification of polyethersulfone membranes by plasma polymerisation of acrylic acid monomers and by immobilization of arginine-glycine-aspartic acid (RGD) peptide through a hydrophilic "spacer arm" molecule. The performance of the modified and unmodified membranes was tested by evaluation of the liver function expression of primary human hepatocytes in terms of albumin production, protein secretion and drug biotransformation.
De Bartolo
Loredana
L
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci, cubo 17/C, 1-87030 Rende (CS), Italy. l.debartolo@itm.cnr.it
Morelli
Sabrina
S
Piscioneri
Antonella
A
Lopez
Linda C
LC
Favia
Pietro
P
d'Agostino
Riccardo
R
Drioli
Enrico
E
D001577:Benzophenones; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D049109:Cell Proliferation; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D007659:Ketones / Q000737:chemistry*; D019164:Liver, Artificial; D008422:Materials Testing; D008567:Membranes, Artificial; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*
D001577:Benzophenones; D001672:Biocompatible Materials; D007659:Ketones; D008567:Membranes, Artificial; D011108:Polymers; C063834:polyetheretherketone; D011092:Polyethylene Glycols