P-glycoprotein effect on the properties of its natural lipid environment probed by Raman spectroscopy and Langmuir-Blodgett technique.
Behavior of P-glycoprotein (Pgp) natural lipid environment within the membrane of CEM cells expressing Pgp in the quantities varying from 0% to 32% of the total amount of all membrane proteins is described for the first time. Observed cooperative effect of Pgp-induced increase of membrane stability, decrease of the temperature of gel-to-crystal lipids transition and predominance of the lipid liquid crystalline phase at physiological temperatures should have an impact in development of multidrug resistance phenotype of tumor cells by favoring the Pgp intercellular transfer and Pgp ATPase activity.
Oleinikov
Vladimir A
VA
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. Miklukho-Maklaya, 117997 Moscow, Russia. voleinik@mail.ru
Fleury
Fabrice
F
Ianoul
Anatoli
A
Zaitsev
Sergey
S
Nabiev
Igor
I
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D013696:Temperature
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008055:Lipids