Culture medium modulates proinflammatory conditions of human pancreatic islets before transplantation.
A portion of transplanted islets is lost during engraftment as a result of stressful events, involving hypoxia and production of proinflammatory molecules by islets. Two of these molecules (monocyte chemoattractant protein-1, CCL2/MCP-1 and tissue factor, TF) are directly correlated with reduced graft function. We evaluated which factors reduce islet proinflammatory conditions. In particular the effects of different culture media supplemented with proteins or antioxidant agents on CCL2/MCP-1 and TF human islet release were evaluated. We observed that human islets after culture in final wash culture medium (FW) significantly decreased CCL2/MCP-1 release and TF production compared with CMRL and M199. These effects were independent from the type of protein added to the media (human serum, human albumin, fetal calf serum). Glutathione in FW further decreased CCL2/MCP-1 in a dose-dependent manner. Culture conditions can modulate the proinflammatory state of islets, and could be used in clinical islet transplantation to reduce inflammation during engraftment.
Marzorati
S
S
Unit of Cell Therapy for Type 1 Diabetes Mellitus, San Raffaele Scientific Institute, Milan, Italy.
Antonioli
B
B
Nano
R
R
Maffi
P
P
Piemonti
L
L
Giliola
C
C
Secchi
A
A
Lakey
J R
JR
Bertuzzi
F
F
D000596:Amino Acids / Q000032:analysis; D000975:Antioxidants / Q000494:pharmacology; D002102:Cadaver; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D006801:Humans; D007249:Inflammation; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D016381:Islets of Langerhans Transplantation / Q000473:pathology / Q000502:physiology*; D012492:Salts / Q000032:analysis; D014019:Tissue Donors; D014815:Vitamins / Q000032:analysis
D000596:Amino Acids; D000975:Antioxidants; D003470:Culture Media; D007328:Insulin; D012492:Salts; D014815:Vitamins