SSLP-1, a secreted Ly-6 protein purified from mouse seminal vesicle fluid.
The Ly-6 protein family refers to a group of glycophosphatidyl inositol-anchored membrane proteins with ten conserved cysteines. They are thought to be involved in cellular adhesion and signaling. Recently, a subfamily of secreted Ly-6 proteins has been identified. In the present study, we report a secreted Ly-6 protein, secreted seminal vesicle Ly-6 protein 1 (SSLP-1) purified from mouse seminal vesicles using a series of steps including ion-exchange chromatography on a diethylaminoethyl (DEAE)-Sephacel column, gel filtration on a Sephadex G-75 column, and ion-exchange HPLC on a sulfopropyl column. Further analysis demonstrated it to be a novel, previously unnamed, 17 kDa glycoprotein. N-glycosidase F treatment revealed a core protein with a molecular mass of 8720 Da. By Basic Local Alignment Search Tool Protein analysis, we found that SSLP-1 had ten conserved cysteine residues identical with other secreted Ly-6 proteins. The gene Gm191, which is located on chromosome 9, encodes SSLP-1. By Northern blotting with 21 different mouse tissues, we found that Sslp-1 mRNA was predominantly expressed in the seminal vesicle. Immunohistochemistry revealed SSLP-1 protein in the luminal fluid and mucosal epithelium of the seminal vesicles. The amount of Sslp-1 mRNA and SSLP-1 protein in the seminal vesicle was regulated by testosterone and correlated with the stage of animal maturation. The tissue-specific expression pattern suggests that SSLP-1 may play a physiological role in male mouse reproduction.
Li
Sheng-Hsiang
SH
Department of Medical Research, Mackay Memorial Hospital, Tamshui 251, Taiwan, ROC. lsh@ms1.mmh.org.tw
Lee
Robert Kuo-Kuang
RK
Lin
Ming-Huei
MH
Hwu
Yuh-Ming
YM
Lu
Chung-Hao
CH
Chen
Ying-Jie
YJ
Chen
Hsuan-Chiang
HC
Chang
Wen-Hwei
WH
Chang
Wei-Chao
WC
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis* / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D015870:Gene Expression / Q000187:drug effects; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D012661:Semen / Q000737:chemistry*; D012669:Seminal Vesicles / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment; D013739:Testosterone / Q000494:pharmacology
D000950:Antigens, Ly; D012333:RNA, Messenger; C520154:SSLP-1 protein, mouse; D013739:Testosterone