Decreased expression of thyroid receptor-associated protein 220 in temporal lobe tissue of patients with refractory epilepsy.
OBJECTIVE
TRAP220 (thyroid hormone receptor-associated protein) functions as a coactivator for nuclear receptors and stimulates transcription by recruiting the TRAP mediator complex to hormone responsive promoter regions. Thus, TRAP220 enhances the function of thyroid/steroid hormone receptors such as thyroid hormone and oestrogen receptors. This study investigated the expression of TRAP220 mRNA and protein level in epileptic brains comparing with human control.


METHODS
We examined the expression of TRAP220 mRNA and protein levels in temporal lobes from patients with chronic pharmacoresistant epilepsy who have undergone surgery.


RESULTS
Expression of TRAP220 mRNA and protein was shown to be decreased significantly in the temporal cortex of the patients with epilepsy.


CONCLUSIONS
Our work showed that a decrease in TRAP220 mRNA and protein levels may be involved in the pathophysiology of epilepsy and may be associated with impairment of the brain caused by frequent seizures.
Li
Jin-Mei
JM
Neurology Department, The First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 4000162, China.
Wang
Xue-Feng
XF
Xi
Zhi-Qin
ZQ
Gong
Yun
Y
Liu
Feng-Ying
FY
Sun
Ji-Jun
JJ
Wu
Yuan
Y
Luan
Guo-Ming
GM
Wang
Yu-Ping
YP
Li
Yun-Lin
YL
Zhang
Jian-Guo
JG
Lu
Yong
Y
Li
Hong-Wei
HW
D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D002648:Child; D015536:Down-Regulation; D004827:Epilepsy / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D056913:Mediator Complex Subunit 1; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D013702:Temporal Lobe / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
C538855:MED1 protein, human; D056913:Mediator Complex Subunit 1; D012333:RNA, Messenger; D014157:Transcription Factors