[VNTR polymorphism of C6orf37 in Chinese population].
To identify a novel VNTR in C6orf37 and to detect the C6orf37 VNTR polymorphism distribution in Chinese population.



RT-PCR and sequencing were conducted to identify VNTR alleles in the variable region of C6orf37.SSLP and DHPLC were applied in detecting the VNTR genotypes in 166 Chinese individuals.



A novel VNTR sequence was found in the second exon of C6orf37, which was composed of 15 base pairs encoding 5-amino-acid (G-G-D-F-G). The repeat times ranged from 3 to 5. There were three common alleles containing three repeats (a), four repeats (b) and five repeats (c), respectively, which produced three homozygotes (a/a, b/b and c/c) and three heterozygotes (a/b, a/c and b/c). The frequency of a, b, c alleles were 0.145, 0.304, 0.551, respectively in Chinese population. Heterozygosity (H) was 0.583. Polymorphism information content (PIC) was 0.510. The screened result of DHPLC was consistent with that of SSLP.



A novel highly polymorphic VNTR in C6orf37 exists in Chinese population. DHPLC is the most efficient technique for screening VNTR polymorphism.
Cui
Jing
J
Department of Pathology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Wang
Wei
W
Wang
Min
M
Lin
Jie
J
Ma
Yu
Y
Ruan
Wen-jing
WJ
Xu
Jing
J
Lai
Mao-de
MD
D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology; D005091:Exons / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics*; D011062:Polynucleotide Adenylyltransferase; D011506:Proteins / Q000235:genetics*
D011506:Proteins; D011062:Polynucleotide Adenylyltransferase; C466099:TENT5A protein, human