[Physico-chemical properties of staphylococci of different species in resistance to human thrombodefensins].
Testing 54 strains of staphylococci (Staphylococcus aureus, S. epidermidis, S. haemolyticus, S. warneri, S. hominis, S. capitis) revealed that S. aureus in contrast to coagulase-negative staphylococci (CNS) is more resistant to bactoriocidal action of human thrombodefensins (resistance index: 60.3 vs 25.6%), less hydrophilicolipophilic balance-HLB: -0.42 vs -0.64) and less charged (x-potential: -32.4 vs -35.6 mV). In groups of staphylococci (S. aureus and CNS) correlation links of bacterial resistance to human thrombodefensins with their HLB and x-potential (r=-0.32...-0.36). By In vitro experiments, it was shown that 5 passages of staphylococci in meat-peptonic broth with human thrombodefensins (50 mkg protein/ml) lead to adaptation of bacteria followed by the formation of resistance to cationic peptides from thrombocytes, a decrease of hydrophobicity and x-potencial. The role of physico-chemical properties in providing thrombodefensin-resistance of staphylococci as a developmental factor of infectious-and-inflammatory process and persistence of bacteria was confirmed with Salmonella infection.
Gritsenko
V A
VA
Ivanov
Iu B
IuB
Zhurlov
O S
OS
D000222:Adaptation, Physiological; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001792:Blood Platelets / Q000737:chemistry*; D003030:Coagulase; D023082:Defensins / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004553:Electric Conductivity; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D012692:Serial Passage; D013045:Species Specificity; D013210:Staphylococcus / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology
D000890:Anti-Infective Agents; D003030:Coagulase; D023082:Defensins