title: Members of adenovirus species B utilize CD80 and CD86 as cellular attachment receptors.
abstract: Alternate serotypes of adenovirus (Ad), including Ads of species B, are being explored to circumvent the disadvantages of Ad serotype 5 gene delivery vectors. Whereas the majority of human Ads utilize the Coxsackievirus and adenovirus receptor (CAR), none of the Ad species B use CAR. Ad species B is further divided into two subspecies, B1 and B2, and utilizes at least two classes of receptors: common Ad species B receptors and B2 specific receptors. CD46 has been implicated as a B2-specific receptor. Ad serotype 3 (Ad3), a member of B1, utilizes CD80 and CD86 as cellular attachment receptors. The receptor-interacting Ad fiber-knob domain is highly homologous among species B Ads. We hypothesized that other members of Ad species B may utilize CD80 and CD86 as cellular attachment receptors. All tested species B members showed specific binding to cells expressing CD80 and CD86, and the Ad fiber-knob domain from both B1 and B2 Ad efficiently blocked CD80- and CD86-mediated infection of Ad3 vectors. Members of both B1 and B2 demonstrated CD80- and CD86-specific infection of CHO cells expressing CD80 and CD86. Therefore, all of the members of Ad species B utilize CD80 and CD86 for infection of cells.
authors.affiliation: Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, and Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL 35294-2172, USA.
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Short
authors.forename: Chenthamarakshan
authors.initials: C
authors.lastname: Vasu
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Holterman
authors.forename: David T
authors.initials: DT
authors.lastname: Curiel
authors.forename: Alexander
authors.initials: A
authors.lastname: Pereboev
mesh_terms: D000256:Adenoviridae / Q000502:physiology*; D000818:Animals; D018122:B7-1 Antigen / Q000502:physiology*; D051940:B7-2 Antigen / Q000502:physiology*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D011991:Receptors, Virus / Q000502:physiology*; D053585:Virus Attachment
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D011991:Receptors, Virus