NORMA: a tool for flexible fitting of high-resolution protein structures into low-resolution electron-microscopy-derived density maps.
This paper describes a freely available software suite that allows the modelling of large conformational changes of high-resolution three-dimensional protein structures under the constraint of a low-resolution electron-density map. Typical applications are the interpretation of electron-microscopy data using atomic scale X-ray structural models. The software package provided should enable the interested user to perform flexible fitting on new cases without encountering major technical difficulties. The NORMA software suite including three fully executable reference cases and extensive user instructions are available at http://www.elnemo.org/NORMA/.
Suhre
Karsten
K
Genomics and Structural Information Laboratory, Institut de Biologie Structurale et Microbiologie, UPR 2589 CNRS, Marseille, France. karsten.suhre@igs.cnrs-mrs.fr
Navaza
Jorge
J
Sanejouand
Yves Henri
YH
D000465:Algorithms; D018834:Chaperonin 60 / Q000737:chemistry; D019295:Computational Biology / Q000379:methods*; D003201:Computers; D004583:Electrons; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron; D008968:Molecular Conformation; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D012984:Software
D018834:Chaperonin 60; D011506:Proteins