title: [Expression and activation of nuclear factor-kappaB in middle ear cholesteatoma].
abstract: OBJECTIVE
  To explore the expression and activation of NF-kappaB in middle ear cholesteatoma.
  
  
  METHODS
  The protein expression of NF-kappaB p65 in 21 middle ear cholesteatoma tissues and 8 normal external ear canal skin obtained in middle ear surgery were examined by immunohistochemistry; NF-kappaB DNA binding activity in these two kinds of tissues were also detected by electrophoretic motility shift assay (EMSA). The influence of cholesteatoma debris on the NF-kappaB DNA binding activity of HaCat cell were further analyzed.
  
  
  RESULTS
  All epithelial cell of cholesteatoma revealed a relatively abundant plasma expression of NF-kappaB p65 protein, among which 12 cases showed nuclear positive expression. In contrast,the normal skin epithelium only revealed a sparse plasma distribution of NF-kappaB protein. The levels of NF-kappaB p65 protein expression in the epithelium of middle ear cholesteatoma tissue and normal skin were 0.168 +/- 0.051, 0.088 +/- 0.019 (t = 4.211, P < 0.01), respectively. The NF-kappaB DNA binding activities of cholesteatoma [(16.5 +/- 10.1)%] were also higher than those in normal skin [(1.38 +/- 1.24)%, t = 3. 600, P = 0.014]. The NF-kappaB DNA binding activity of HaCat cell increased when exposed to cholesteatoma debris in a dose dependent manner.
  
  
  CONCLUSIONS
  NF-kappaB might be an important factor which was involved in the occurrence and development of cholesteatoma.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Renmin Hospital, Wuhan University, Wuhan 430060, China. xy37138@163.com
authors.forename: Yu
authors.initials: Y
authors.lastname: Xu
authors.forename: Ze-zhang
authors.initials: ZZ
authors.lastname: Tao
authors.forename: Qing-quan
authors.initials: QQ
authors.lastname: Hua
authors.forename: Xin-xhun
authors.initials: XX
authors.lastname: Wang
authors.forename: Bo-kui
authors.initials: BK
authors.lastname: Xiao
mesh_terms: D000293:Adolescent; D000328:Adult; D001706:Biopsy; D016022:Case-Control Studies; D002648:Child; D018424:Cholesteatoma, Middle Ear / Q000378:metabolism* / Q000473:pathology*; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism*; D055815:Young Adult
chemical_list: D016328:NF-kappa B