title: A chip-based miniaturized format for protein-expression profiling: the exploitation of comprehensively produced antibodies.
abstract: Numerous antibodies have been developed and validated in recent years, and show promise for use in novel functional protein assays. Such assays would be an alternative to pre-existing comprehensive assays, such as DNA microarrays. Antibody microarrays are thought to represent those functional protein assays. While a variety of attempts have been made to apply DNA microarray technology to antibody microarrays, a fully optimized protocol has not been established. We have been conducting a project to comprehensively produce antibodies against mouse KIAA ("KI" stands for "Kazusa DNA Research Institute" and "AA" are reference characters) proteins. Using our library of antibodies, we established a novel antibody microarray format that utilizes surface plasmon resonance (SPR) technology. A label-free real-time measurement of protein expression in crude cell lysates was achieved by direct readout of the bindings using SPR. Further refinement of the antibody microarray format enabled us to detect a smaller quantity of target proteins in the lysate without the bulk effect. In this review, we first summarize available antibody array formats and then describe the above-mentioned format utilizing updated SPR technology.
authors.affiliation: Chiba Industry Advancement Center, Chiba, Japan. hkoga@kazusa.or.jp
authors.forename: Hisashi
authors.initials: H
authors.lastname: Koga
authors.forename: Motoki
authors.initials: M
authors.lastname: Kyo
authors.forename: Kazue
authors.initials: K
authors.lastname: Usui-Aoki
authors.forename: Kazuki
authors.initials: K
authors.lastname: Inamori
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008904:Miniaturization; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D011506:Proteins