High-throughput analysis of catechins and theaflavins by high performance liquid chromatography with diode array detection.
Increased interest in potential health-protective activities of flavonoid-rich tea has created the need to take advantage of HPLC column and system advances in order to optimize methodologies for flavonoid analysis. Two new RP-C18 methods for HPLC-DAD analysis of tea flavonoids were developed to facilitate separation of catechins within 5 min and separation of catechins and theaflavins within 10 min total analysis time. Calibration results indicate that these methods have on-column limits of detection on the order of 1-10 pmol for most tea catechins, and method replication generally resulted in intraday and interday peak area variation of <5% for catechins and <9% for theaflavins in green and black tea infusions. These new methods are therefore sensitive, reproducible, and represent a 2-4-fold reduction in HPLC analysis time from existing analytical methods. These improvements are readily achievable with commonly used HPLC equipment, thus facilitating increased sample throughput and efficiency across a broad range of experimental applications.
Neilson
Andrew P
AP
Department of Food Science, Purdue University, 745 Agriculture Mall Dr. West Lafayette, IN 47907, United States.
Green
Rodney J
RJ
Wood
Karl V
KV
Ferruzzi
Mario G
MG
D044946:Biflavonoids / Q000032:analysis* / Q000737:chemistry; D002392:Catechin / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013662:Tea / Q000737:chemistry*
D044946:Biflavonoids; D013662:Tea; C056068:theaflavin; D002392:Catechin