Preparative isolation of hydrolysable tannins chebulagic acid and chebulinic acid from Terminalia chebula by high-speed counter-current chromatography.
As a chromatographic column, the high-speed counter-current chromatography system was equipped with a preparative HPLC series, enabling the successful isolation of hydrolysable tannins from the fruits of Terminalia chebula, a traditional Chinese medicine. The two-phase solvent system was composed of n-hexane-ethyl acetate-methanol-water (1:20:1:20 v/v). As a result, 33.2 mg chebulagic and 15.8 mg chebulinic acids were obtained in one step from 300 mg of crude extract. Their purities were determined by HPLC to be 95.3 and 96.1%, respectively. The chemical structures were identified by their MS and 1H NMR spectra.
Han
Quanbin
Q
Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Shatin N. T., Hong Kong, China.
Song
Jingzheng
J
Qiao
Chunfeng
C
Wong
Lina
L
Xu
Hongxi
H
D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D047348:Hydrolyzable Tannins / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012997:Solvents; D016339:Spectrometry, Mass, Fast Atom Bombardment; D029766:Terminalia / Q000737:chemistry*
D001578:Benzopyrans; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D047348:Hydrolyzable Tannins; D012997:Solvents; C103481:chebulinic acid; C076178:chebulagic acid