title: [Sensitivity profile of a panel of cell lines designed for the evaluation of in vitro cytotoxicity].
abstract: BACKGROUND
  Preliminary in vitro cytoxicity evaluations are determined in human tumor cell lines as a bioassay for the screening of potentially anticancer natural products.
  
  
  OBJECTIVE
  To strengthen the available in vitro cytotoxicity evaluation models, the panel of cell lines was expanded, and the sensitivity profile of each cell line was evaluated for its response to selected antineoplasic drugs.
  
  
  METHODS
  HeLa, MKN-45 and U-937 cell lines were added to the panel, and the sensitivity was determined for each of seven cell lines: HEp-2, HT-29, MCF-7, SiHa, MKN-45, HeLa and U-937. The effects of the antineoplasic drugs Doxorubicin HCl, Taxol, Cisplatin, Cyclophosphamide and Carmustin were examined, using the methyl thiazol tetrazolium (MTT) reduction assay.
  
  
  RESULTS
  A differential sensitivity to the drugs Doxorubicin HCl, Taxol and Cisplatin was established among the cell lines by comparing the lethal concentration 50 (LC50) values. HEp-2 was the most sensitive cell line, whereas HeLa and U-937 were the most resistant. HEp-2 exhibited a biphasic response to Taxol treatment; this was related to the reported mechanism of action of this compound.
  
  
  CONCLUSIONS
  Cyclophosphamide and Carmustin did not show activity under test conditions.
authors.affiliation: Grupo de Farmacogenética del Cáncer, Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, D.C., Colombia.
authors.forename: Claudia Jhoana
authors.initials: CJ
authors.lastname: León
authors.forename: Sandra Milena
authors.initials: SM
authors.lastname: Gómez
authors.forename: Sandra Johanna
authors.initials: SJ
authors.lastname: Morantes
authors.forename: Claudia Patricia
authors.initials: CP
authors.lastname: Cordero
authors.forename: Fabio Ancízar
authors.initials: FA
authors.lastname: Aristizábal
mesh_terms: D000970:Antineoplastic Agents / Q000633:toxicity*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D012680:Sensitivity and Specificity
chemical_list: D000970:Antineoplastic Agents