Quality assessment and analysis of Biogen Idec compound library.
A subset of the compound repository for lead identification at Biogen Idec was characterized for its chemical stability over a 3-year period. Compounds were stored at 4 degrees C as 10 mM DMSO stocks, and a small subset of compounds was stored as lyophilized dry films. Compound integrity of 470 discrete compounds (Compound Set I) and 1917 combinatorial chemistry-derived compounds (Compound Set II) was evaluated by liquid chromatography/mass spectrometry from the time of acquisition into the library collection and after 3 years of storage. Loss of compound integrity over the 3 years of storage was observed across the 2 subsets tested. Of Compound Set I, 63% of samples retained > 80% purity, whereas 57% of samples from Compound Set II had purity greater than 60%. The stability of the lyophilized samples was superior to the samples stored as DMSO solution. Although storage at 4 degrees C as DMSO solution was adequate for the majority of compounds, the authors observed and quantified the level of degradation within the compound collection. Their study provides general insight into compound storage and selection of library subsets for future lead identification activities.
Bowes
Scott
S
Biogen Idec, Cambridge, MA 02142, USA.
Sun
Dongyu
D
Kaffashan
Azita
A
Zeng
Chenhui
C
Chuaqui
Claudio
C
Hronowski
Xiaoping
X
Buko
Alex
A
Zhang
Xin
X
Josiah
Serene
S
D002853:Chromatography, Liquid; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004355:Drug Stability; D004356:Drug Storage; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry / Q000592:standards*; D011786:Quality Control; D013997:Time Factors
D004364:Pharmaceutical Preparations