Transient isotachophoresis in carrier ampholyte-based capillary electrophoresis for protein analysis.
Transient ITP (t-ITP) has been used in carrier ampholyte-based CE (CABCE) to enhance the sensitivity of protein analysis. The characteristics of carrier ampholytes (CAs) narrow pH cuts-based buffers, when used as BGEs in CE are compatible with t-ITP requirements. Indeed, being the sole buffering species of such solutions, CAs impose a pH close to their pI. Thus, in these solutions, the CAs possess low electrophoretic mobility. As a consequence, by adding an ionic component with high electrophoretic mobility either in the studied sample or in the BGE, a t-ITP step can be generated. This has first been demonstrated for protein test mixtures. Then, the combination of t-ITP with CABCE has been applied to study a real sample, the bovine milk.
Busnel
Jean-Marc
JM
Laboratory of Environment and Analytical Chemistry, UMR CNRS 7121, ESPCI, Paris, France. jean-marc.bunsel@espci.fr
Descroix
Stéphanie
S
Godfrin
Dominique
D
Hennion
Marie-Claire
MC
Kasicka
Václav
V
Peltre
Gabriel
G
D000665:Ampholyte Mixtures / Q000737:chemistry*; D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D008892:Milk / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D012996:Solutions / Q000737:chemistry
D000665:Ampholyte Mixtures; D011506:Proteins; D012996:Solutions