Novel streptomycin resistance gene from Mycobacterium fortuitum.
We have isolated the aph(3")-Ic gene, encoding an aminoglycoside 3"-O-phosphotransferase [APH(3")-Ic], from a genomic library of an environmental Mycobacterium fortuitum strain, selecting for streptomycin resistance. APH(3")-Ic phosphorylates and inactivates streptomycin. Similar genes have been described in Streptomyces griseus and plasmid RSF1010. It is also present in some M. fortuitum clinical isolates.
Ramón-García
Santiago
S
Departamento de Microbiología, Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad de Zaragoza, C/ Domingo Miral s/n, 50009-Zaragoza, Spain.
Otal
Isabel
I
Martín
Carlos
C
Gómez-Lus
Rafael
R
Aínsa
José A
JA
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D029968:Escherichia coli Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000187:drug effects; D019907:Mycobacterium fortuitum / Q000187:drug effects* / Q000235:genetics*; D020102:Mycobacterium smegmatis / Q000187:drug effects; D010957:Plasmids / Q000235:genetics; D013307:Streptomycin / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; C104889:mraZ protein, E coli; D013307:Streptomycin