title: Heterozygous HIF-1alpha deficiency impairs carotid body-mediated systemic responses and reactive oxygen species generation in mice exposed to intermittent hypoxia.
abstract: Chronic intermittent hypoxia (CIH) occurs in patients with sleep apnoea and has adverse effects on multiple physiological functions. Previous studies have shown that reflexes arising from carotid bodies mediate CIH-evoked cardio-respiratory responses, and reactive oxygen species (ROS) play important roles in eliciting systemic responses to CIH. Very little is known about the molecular mechanisms underlying CIH. The transcriptional activator hypoxia-inducible factor-1 (HIF-1) mediates a broad range of cellular and systemic responses to hypoxia, and HIF-1 is activated in cell cultures exposed to IH. In the present study we examined whether CIH activates HIF-1 and if so whether it contributes to cardio-respiratory responses and ROS generation in mice. Experiments were performed on male littermate wild-type (WT) and heterozygous (HET) mice partially deficient in HIF-1alpha, the O2 regulated subunit of the HIF-1 complex. Both groups of mice were exposed to either 10 days of CIH (15 s of hypoxia followed by 5 min of normoxia, 9 episodes h-1, 8 h day-1) or to 10 days of 21% O2 (controls). Carotid body response to hypoxia was augmented, and acute intermittent hypoxia (AIH) induced sensory long-term facilitation (sLTF) of the chemoreceptor activity in CIH-exposed WT mice. In striking contrast, hypoxic sensory response was unaffected and AIH was ineffective in eliciting sLTF in CIH-exposed HET mice. Analysis of cardio-respiratory responses in CIH-exposed WT mice revealed augmented hypoxic ventilatory response, LTF of breathing, elevated blood pressures and increased plasma noradrenaline. In striking contrast these responses were either absent or attenuated in HET mice exposed to CIH. In CIH-exposed WT mice, ROS were elevated and this response was absent in HET mice. Manganese (III) tetrakis(1-methyl-4-pyridyl) porphyrin pentachloride, a potent scavenger of superoxide, not only prevented CIH-induced increases in ROS but also CIH-evoked HIF-1alpha up-regulation in WT mice. These results indicate that: (a) HIF-1 activation is critical for eliciting CIH-induced carotid body-mediated cardio-respiratory responses; (b) CIH increases ROS; and (c) the effects of CIH involve complex positive interactions between HIF-1 and ROS.
authors.affiliation: Department of Physiology & Biophysics, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44109, USA.
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Peng
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Yuan
authors.forename: Deviprasadh
authors.initials: D
authors.lastname: Ramakrishnan
authors.forename: Suresh D
authors.initials: SD
authors.lastname: Sharma
authors.forename: Marta
authors.initials: M
authors.lastname: Bosch-Marce
authors.forename: Ganesh K
authors.initials: GK
authors.lastname: Kumar
authors.forename: Gregg L
authors.initials: GL
authors.lastname: Semenza
authors.forename: Nanduri R
authors.initials: NR
authors.lastname: Prabhakar
mesh_terms: D000200:Action Potentials; D000818:Animals; D001794:Blood Pressure; D002320:Cardiovascular Physiological Phenomena; D002344:Carotid Body / Q000503:physiopathology*; D002540:Cerebral Cortex / Q000378:metabolism; D000860:Hypoxia / Q000097:blood / Q000378:metabolism / Q000503:physiopathology*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009431:Neural Conduction; D009473:Neuronal Plasticity; D009638:Norepinephrine / Q000097:blood; D010791:Phrenic Nerve / Q000503:physiopathology; D012123:Pulmonary Ventilation; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012018:Reflex; D012143:Respiratory Physiological Phenomena; D013997:Time Factors
chemical_list: C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D017382:Reactive Oxygen Species; D009638:Norepinephrine