title: [Inhibition of integrin beta1 expression in human keratinocyte stem cells by vector-1 based interfering RNA strategy].
abstract: OBJECTIVE
  To investigate the influence of integrin beta1 on the proliferation and differentiation of human keratinocyte stem cells (KSCs).
  
  
  METHODS
  DNA oligonucleotides targeting integrin beta1 at different locations were synthesized and inserted into BamHI-1 HindIII linearized p Silencer 3.1/H1 plasmids. The inserted sequences were verified by DNA sequencing. The KSCs were divided into control (without transfection), T1 (with transfection of vacant vector), T2 (with transfection of si integrin beta(1-1) vector), T3 (with transfection of si integrin beta(1-1) vector), and T4 (with transfection of si Negative vector) groups. The change in the expression of integrin beta1, was determined with Western blotting. The positive vector with the highest expression of integrin beta1 was selected and named as integrin beta1, and semi-quantitative RT-PCR was employed to detect the change in the expression of integrin beta1 mRNA.
  
  
  RESULTS
  The protein expression of integrin beta1, was not suppressed in control and T1 group, but it was suppressed in T2 and T3 groups, especially in T3 group (the suppression rate was 60%-70%, which was named si integrin beta1). The expression of integrin beta1 mRNA was obviously decreased by integrin beta1, transfection (the suppression rate was 70%).
  
  
  CONCLUSIONS
  The expression of integrin beta1, mRNA and protein could be down-regulated with recombinant si integrin P, vector transfection.
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Hong-ying
authors.initials: HY
authors.lastname: Tang
authors.forename: Su-hua
authors.initials: SH
authors.lastname: Wu
authors.forename: Yong-yue
authors.initials: YY
authors.lastname: Su
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Yue-ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Guang-ping
authors.initials: GP
authors.lastname: Liang
authors.forename: Rong-ju
authors.initials: RJ
authors.lastname: Sun
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Luo
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005822:Genetic Vectors; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D013234:Stem Cells / Q000378:metabolism*; D014162:Transfection
chemical_list: D019012:Integrin beta1; D012333:RNA, Messenger; D034741:RNA, Small Interfering