Development- and activity-dependent regulation of SNAP-25 phosphorylation in rat brain.
Synaptosomal-associated protein of 25kDa (SNAP-25), a member of the SNARE proteins essential for neurotransmitter release, is phosphorylated at Ser(187) in PC12 cells and in the rat brain in a protein kinase C-dependent manner. It remains unclear how the phosphorylation of SNAP-25 is regulated during development and by neuronal activity. We studied the mode of SNAP-25 phosphorylation at Ser(187) in the rat brain using an anti-phosphorylated SNAP-25 antibody. Both the expression and phosphorylation of SNAP-25 increased remarkably during the early postnatal period, but their onsets were quite different. SNAP-25 expression was detected as early as embryonic Day 18, whereas the phosphorylation of SNAP-25 could not be detected until postnatal Day 4. A delay in the onset of phosphorylation was also observed in cultured rat hippocampal neurons. The phosphorylation of SNAP-25 was regulated in a neuronal activity-dependent manner and, in the rat hippocampus, decreased by introducing seizures with kainic acid. These results clearly indicated that the phosphorylation of SNAP-25 at Ser(187) is regulated in development- and neuronal activity-dependent manners, and is likely to play important roles in higher brain functions.
Kataoka
Masakazu
M
Department of Environmental Science and Technology, Faculty of Engineering, Shinshu University, Wakasato 4-17-1, Nagano-shi, Nagano, Japan.
Kuwahara
Reiko
R
Matsuo
Ryota
R
Sekiguchi
Mariko
M
Inokuchi
Kaoru
K
Takahashi
Masami
M
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D006624:Hippocampus / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D007608:Kainic Acid; D008297:Male; D009474:Neurons / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D050825:Synaptosomal-Associated Protein 25 / Q000096:biosynthesis / Q000378:metabolism*
C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25; D012694:Serine; D007608:Kainic Acid