[Transpositional behaviour analysis of Ds element from different insertion sites on chromosome 4 in rice].
Transgenic plants with Ds element distributed over different loci on chromosome 4 (Fig. 1) and the homozygous transformants with Ac transposase gene were established through Agrobacterium-mediated approach. In this study, the plants carrying Ds element from different loci were crossed with the plant carrying Ac transposase individually. The plants of F(1) generation carrying both Ds element and Ac transposase were used to produce the F(2) populations (Table 1). Analysis of the F(2) generation by the PCR method revealed that the excision frequencies of Ds element were higher in the telomeric region of chromosome 4 than in the centromeric region (Fig. 4). These results showed that the insertion site of Ds element has strong effect on its excision frequency. We suggest that the special construct of chromosome near the insertion site of Ds element is related to the excision frequency of the Ds element.
Zhao
Xiao-Ling
XL
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Wang
Jiang
J
Shen
Ge-Zhi
GZ
Wang
Xin-Qi
XQ
Zhang
Jing-Liu
JL
D001665:Binding Sites; D032461:Chromosomes, Plant / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D019895:Transposases / Q000235:genetics
D004251:DNA Transposable Elements; D019895:Transposases