Detection of Enterobacter sakazakii in dried infant milk formula by cationic-magnetic-bead capture.
Enterobacter sakazakii has been associated with life-threatening infections in premature low-birth-weight infants. Contaminated infant milk formula (IMF) has been implicated in cases of E. sakazakii meningitis. Quick and sensitive methods to detect low-level contamination sporadically present in IMF preparations would positively contribute towards risk reduction across the infant formula food chain. Here we report on the development of a simple method, combining charged separation and growth on selective agar, to detect E. sakazakii in IMF. This protocol can reliably detect 1 to 5 CFU of E. sakazakii in 500 g of IMF in less than 24 h.
Mullane
N R
NR
Centre for Food Safety, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
Murray
J
J
Drudy
D
D
Prentice
N
N
Whyte
P
P
Wall
P G
PG
Parton
A
A
Fanning
S
S
D001431:Bacteriological Techniques / Q000295:instrumentation; D002412:Cations; D015169:Colony Count, Microbial; D044083:Cronobacter sakazakii / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D004755:Enterobacteriaceae / Q000302:isolation & purification; D005516:Food Microbiology; D005519:Food Preservation; D006801:Humans; D041943:Infant Formula; D007231:Infant, Newborn; D008280:Magnetics; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000302:isolation & purification
D002412:Cations