Somatic microindels: analysis in mouse soma and comparison with the human germline.
Microindels, defined as mutations that result in a colocalized microinsertion and microdeletion with a net gain or loss of between 1 and 50 nucleotides, may be an important contributor to cancer. We report the first comprehensive analysis of somatic microindels. Our large database of mutations in the lacI transgene of Big Blue((R)) mice contains 0.5% microindels, 2.8% pure microinsertions, and 11.5% pure microdeletions. There appears to be no age, gender, or tissue-type specificity in the frequency of microindels. Of the independent somatic mutations that result in a net in-frame insertion or deletion, microindels are responsible for 13% of protein expansions and 6% of protein contractions. These in-frame microindels may play a crucial role in oncogenesis and evolution via "protein tinkering" (i.e., modest expansion or contraction of proteins). Four characteristics suggest that microindels are caused by unique mechanisms, not just simple combinations of the same mechanisms that cause pure microinsertions and pure microdeletions. First, microinsertions and microdeletions commonly occur at hotspots, but none of the 30 microindels are recurrent. Second, the sizes of the deletions and insertions in microindels are larger and more varied than in pure microdeletions and pure microinsertions. Third, microinsertions overwhelmingly repeat the adjacent base (97%) while the insertions in microindels do so only infrequently (17%). Fourth, analysis of the sequence contexts of microindels is consistent with unique mechanisms including recruitment of translesion DNA synthesis polymerases. The mouse somatic microindels have characteristics similar to those of human germline microindels, consistent with similar causative mechanisms in mouse and human, and in soma and germline.
Gonzalez
Kelly D
KD
Department of Molecular Genetics, City of Hope National Medical Center, Duarte, California.
Hill
Kathleen A
KA
Li
Kai
K
Li
Wenyan
W
Scaringe
William A
WA
Wang
Ji-Cheng
JC
Gu
Dongqing
D
Sommer
Steve S
SS
D000367:Age Factors; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D005057:Eukaryotic Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D005260:Female; D017353:Gene Deletion; D005854:Germ Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D018095:Germ-Line Mutation; D006801:Humans; D056968:Lac Repressors; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000502:physiology; D009154:Mutation; D009690:Nucleic Acid Conformation; D009928:Organ Specificity; D012097:Repressor Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D012727:Sex Characteristics
D001426:Bacterial Proteins; D056968:Lac Repressors; D012097:Repressor Proteins