Cryptococcus mujuensis sp. nov. and Cryptococcus cuniculi sp. nov., basidiomycetous yeasts isolated from wild rabbit faeces.
Two previously undescribed anamorphic yeasts, strains T-11(T) and T-26(T), recovered from wild rabbit faecal pellets collected in Muju, Korea, were identified using phenotypic and molecular taxonomic methods. The isolates were characterized by the proliferation of budding cells, positive diazonium blue B and urease reactions, the presence of Q-10 as the major ubiquinone, the presence of xylose in whole-cell hydrolysates and the inability to ferment sugars. Phylogenetic analyses based on 26S rRNA gene partial sequences revealed that strain T-11(T) was located in the Bulleromyces clade and was related to Sirobasidium intermedium, Tremella exigua, Cryptococcus cellulolyticus and Bullera pseudoalba. Strain T-26(T) was located in the Mesenterica clade and was closely related to Cryptococcus sp. F6 and Cryptococcus heveanensis CBS 8976. Sequence divergence values of more than 4 % from other described Cryptococcus species, together with the phenotypic differences, showed that the isolated yeasts represent previously unrecognized members of this genus. Therefore, two novel yeast species are proposed: Cryptococcus mujuensis sp. nov., with strain T-11(T) (=KCTC 17231(T)=CBS 10308(T)) as the type strain, and Cryptococcus cuniculi sp. nov., with strain T-26(T) (=KCTC 17232(T)=CBS 10309(T)) as the type strain.
Shin
Kee-Sun
KS
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejon 305-333, Republic of Korea.
Oh
Hee-Mock
HM
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejon 305-333, Republic of Korea.
Park
Yong-Ha
YH
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejon 305-333, Republic of Korea.
Lee
Kang Hyun
KH
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejon 305-333, Republic of Korea.
Poo
Haryoung
H
Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejon 305-333, Republic of Korea.
Kwon
Gi-Seok
GS
The School of Bioresource Science, Andong National University, Andong 760-749, Republic of Korea.
Kwon
O-Yu
OY
Department of Anatomy, School of Medicine, Chungnam National University, Daejon 301-131, Republic of Korea.
D000818:Animals; D001483:Base Sequence; D003454:Cryptococcus / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D004275:DNA, Ribosomal / Q000032:analysis*; D005243:Feces / Q000382:microbiology*; D007723:Korea; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000032:analysis* / Q000235:genetics; D011817:Rabbits
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S