title: Novel tertiary amine oxide surfaces that resist nonspecific protein adsorption.
abstract: Novel surfaces derivatized with tertiary amine oxides have been prepared and tested for their ability to resist nonspecific protein adsorption. The oxidation of tertiary amines supported on triazine units was carried out using mCPBA to give a format allowing conjugation of biologically active ligands alongside them. Adsorption to these surfaces was tested and compared to adsorption to a set of commercial and custom oligo-/poly(ethylene glycol) (OEG/PEG) supports by challenging them with a protein display library presented on bacteriophage lambda. The new class of amine oxide surfaces is found to compare favorably with the performance of the OEG/PEG supports in the prevention of nonspecific binding.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, UK.
authors.forename: Suzanne J
authors.initials: SJ
authors.lastname: Dilly
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Beecham
authors.forename: Steven P
authors.initials: SP
authors.lastname: Brown
authors.forename: John M
authors.initials: JM
authors.lastname: Griffin
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Clark
authors.forename: Craig D
authors.initials: CD
authors.lastname: Griffin
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Marshall
authors.forename: Richard M
authors.initials: RM
authors.lastname: Napier
authors.forename: Paul C
authors.initials: PC
authors.lastname: Taylor
authors.forename: Andrew
authors.initials: A
authors.lastname: Marsh
mesh_terms: D000327:Adsorption; D000588:Amines / Q000737:chemistry*; D008024:Ligands; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D014227:Triazines / Q000737:chemistry
chemical_list: D000588:Amines; D008024:Ligands; D010087:Oxides; D011506:Proteins; D014227:Triazines; D011092:Polyethylene Glycols