title: Downregulation of hnRNP C1/C2 by siRNA sensitizes HeLa cells to various stresses.
abstract: The heterogeneous nuclear ribonucleoprotein C1/C2 is one of the most abundant proteins in the nucleus, and shown to have roles in cellular differentiation and proliferation through post-transcriptional regulations of certain mRNA species. We studied its role in stress response using siRNA mediated knockdown approach in HeLa cells. Upon transient transfection with plasmid encoding siRNA, the cells showed increased sensitivities to various chemical agents, namely H(2)O(2, )paraquat, camptothecin, ICRF-193 and halogenated deoxyuridines. These results demonstrate that hnRNP C1/C2 is involved in maintenance of cellular homeostasis besides cellular differentiation and proliferation.
authors.affiliation: Kihara Institute for Biological research, Yokohama City University, Maioka-cho 641-12, Totsuka-ku, Yokohama 244-0813, Japan.
authors.forename: Mohammad Nazir
authors.initials: MN
authors.lastname: Hossain
authors.forename: Michihiko
authors.initials: M
authors.lastname: Fuji
authors.forename: Kensuke
authors.initials: K
authors.lastname: Miki
authors.forename: Morio
authors.initials: M
authors.lastname: Endoh
authors.forename: Dai
authors.initials: D
authors.lastname: Ayusawa
mesh_terms: D000963:Antimetabolites / Q000378:metabolism; D000970:Antineoplastic Agents / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D002166:Camptothecin / Q000378:metabolism; D054659:Diketopiperazines; D004791:Enzyme Inhibitors / Q000378:metabolism; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006540:Herbicides / Q000378:metabolism; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D010269:Paraquat / Q000378:metabolism; D010879:Piperazines / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*
chemical_list: D000963:Antimetabolites; D000970:Antineoplastic Agents; D054659:Diketopiperazines; D004791:Enzyme Inhibitors; D006540:Herbicides; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C; D016877:Oxidants; D010879:Piperazines; D034741:RNA, Small Interfering; C070899:4,4'-(1,2-dimethyl-1,2-ethanediyl)bis-2,6-piperazinedione; D006861:Hydrogen Peroxide; D001973:Bromodeoxyuridine; D010269:Paraquat; D002166:Camptothecin