Cell cycle regulators control centrosome elimination during oogenesis in Caenorhabditis elegans.
In many animals, the bipolar spindle of the first zygotic division is established after the contribution of centrioles by the sperm at fertilization. To avoid the formation of a multipolar spindle in the zygote, centrosomes are eliminated during oogenesis in most organisms, although the mechanism of this selective elimination is poorly understood. We show that cki-2, a Caenorhabditis elegans cyclin-dependent kinase (Cdk) inhibitor, is required for their appropriate elimination during oogenesis. In the absence of cki-2, embryos have supernumerary centrosomes and form multipolar spindles that result in severe aneuploidy after anaphase of the first division. Moreover, we demonstrate that this defect can be suppressed by reducing cyclin E or Cdk2 levels. This implies that the proper regulation of a cyclin E-Cdk complex by cki-2 is required for the elimination of the centrosome that occurs before or during oogenesis to ensure the assembly of a bipolar spindle in the C. elegans zygote.
Kim
Dae Young
DY
Department of Biology, McGill University, Montreal, Quebec H3A 1B1, Canada.
Roy
Richard
R
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology; D018385:Centrosome / Q000378:metabolism*; D019927:Cyclin E / Q000378:metabolism; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D005804:Genes, Lethal / Q000235:genetics; D018816:Genes, cdc / Q000502:physiology; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D009866:Oogenesis / Q000502:physiology*; D008941:Spindle Apparatus / Q000378:metabolism*
D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D019927:Cyclin E; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; C412838:cki-2 protein, C elegans; D051357:Cyclin-Dependent Kinase 2