title: Molecular characterization of Cryptosporidium spp. from children in Kolkata, India.
abstract: The intracellular parasite Cryptosporidium is responsible for severe diarrhea in immunocompromised persons in developing countries. Few studies on the characterization of the parasite in India are available. In this study, molecular characterization of the parasite from diarrheic children was carried out by PCR-restriction fragment length polymorphism analysis. At least three genotypes were identified. Out of 40 positive samples, 35 were positive for C. hominis, 4 were positive for C. parvum, and 1 was positive for C. felis. This study clearly suggests that cryptosporidiosis in this region is caused largely by anthroponotic transmission.
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences, Agam Kuan, Patna 800 007, India. dasp@cal2.vsnl.net.in
authors.forename: Pradeep
authors.initials: P
authors.lastname: Das
authors.forename: Seuli Saha
authors.initials: SS
authors.lastname: Roy
authors.forename: Kakali
authors.initials: K
authors.lastname: MitraDhar
authors.forename: Phalguni
authors.initials: P
authors.lastname: Dutta
authors.forename: Mihir K
authors.initials: MK
authors.lastname: Bhattacharya
authors.forename: Abhik
authors.initials: A
authors.lastname: Sen
authors.forename: Sandipan
authors.initials: S
authors.lastname: Ganguly
authors.forename: Sujit K
authors.initials: SK
authors.lastname: Bhattacharya
authors.forename: Altaf A
authors.initials: AA
authors.lastname: Lal
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000635:transmission; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics*; D003967:Diarrhea / Q000469:parasitology; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length