title: ANKHD1, ankyrin repeat and KH domain containing 1, is overexpressed in acute leukemias and is associated with SHP2 in K562 cells.
abstract: In the present study, increased levels of ANKHD1 mRNA and protein expression in leukemia cell lines are reported, as compared with normal hematopoietic cells. Furthermore, a higher expression of ANKHD1 mRNA was detected in primary acute leukemia samples than in normal hematopoietic cells (P=0.002). ANKHD1 was detected in the cytosolic and membrane fraction of cells and was co-immunoprecipitated with SHP2 in protein extracts of K562 and LNCaP cell lines. These findings suggest a role for ANKHD1 as a scaffolding protein that may be associated with the abnormal phenotype of leukemia cells.
authors.affiliation: The Hematology and Hemotherapy Center, Faculty of Medical Sciences, Department of Internal Medicine, State University of Campinas, São Paulo, Brazil.
authors.forename: Fabíola
authors.initials: F
authors.lastname: Traina
authors.forename: Patricia M B
authors.initials: PM
authors.lastname: Favaro
authors.forename: Samuel de Souza
authors.initials: Sde S
authors.lastname: Medina
authors.forename: Adriana da Silva Santos
authors.initials: Ada S
authors.lastname: Duarte
authors.forename: Sheila Maria Brochado
authors.initials: SM
authors.lastname: Winnischofer
authors.forename: Fernando F
authors.initials: FF
authors.lastname: Costa
authors.forename: Sara T O
authors.initials: ST
authors.lastname: Saad
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017089:Ankyrin Repeat; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D020014:K562 Cells; D007938:Leukemia / Q000378:metabolism*; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C489948:ANKK1 protein, human; D017346:Protein Serine-Threonine Kinases; C516724:PTPN11 protein, human; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D017027:Protein Tyrosine Phosphatases