title: Epigenetic dysregulation underlies radiation-induced transgenerational genome instability in vivo.
abstract: OBJECTIVE
  Although modern cancer radiation therapy has led to increased patient survival rates, the risk of radiation treatment-related complications is becoming a growing problem. Among various complications, radiation also poses a threat to the progeny of exposed parents. It causes transgenerational genome instability that is linked to transgenerational carcinogenesis. Although the occurrence of transgenerational genome instability, which manifests as elevated delayed and nontargeted mutation, has been well documented, the mechanisms by which it arises remain obscure. We hypothesized that epigenetic alterations may play a pivotal role in the molecular etiology of transgenerational genome instability.
  
  
  METHODS
  We studied the levels of cytosine DNA methylation in somatic tissues of unexposed offspring upon maternal, paternal, or combined parental exposure.
  
  
  RESULTS
  We observed a significant loss of global cytosine DNA methylation in the thymus tissue of the offspring upon combined parental exposure. The loss of DNA methylation was paralleled by a significant decrease in the levels of maintenance (DNMT1) and de novo methyltransferases DNMT3a and 3b and methyl-CpG-binding protein MeCP2. Along with profound changes in DNA methylation, we noted a significant accumulation of DNA strand breaks in thymus, which is a radiation carcinogenesis target organ.
  
  
  CONCLUSIONS
  The observed changes were indicative of a profound epigenetic dysregulation in the offspring, which in turn could lead to genome destabilization and possibly could serve as precursor for transgenerational carcinogenesis. Future studies are clearly needed to address the cellular and carcinogenic repercussions of those changes.
authors.affiliation: Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, Canada.
authors.forename: Igor
authors.initials: I
authors.lastname: Koturbash
authors.forename: Mike
authors.initials: M
authors.lastname: Baker
authors.forename: Jonathan
authors.initials: J
authors.lastname: Loree
authors.forename: Kristy
authors.initials: K
authors.lastname: Kutanzi
authors.forename: Darryl
authors.initials: D
authors.lastname: Hudson
authors.forename: Igor
authors.initials: I
authors.lastname: Pogribny
authors.forename: Olga
authors.initials: O
authors.lastname: Sedelnikova
authors.forename: William
authors.initials: W
authors.lastname: Bonner
authors.forename: Olga
authors.initials: O
authors.lastname: Kovalchuk
mesh_terms: D000818:Animals; D003596:Cytosine / Q000378:metabolism; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism; D004249:DNA Damage; D019175:DNA Methylation / Q000528:radiation effects*; D000090205:DNA Methyltransferase 3A; D044127:Epigenesis, Genetic / Q000528:radiation effects*; D005260:Female; D042822:Genomic Instability / Q000235:genetics*; D006657:Histones / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D018811:Maternal Exposure; D051379:Mice; D008810:Mice, Inbred C57BL; D018812:Paternal Exposure; D011897:Random Allocation; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D014916:Whole-Body Irradiation; D000097584:DNA Methyltransferase 3B
chemical_list: D003596:Cytosine; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; D006657:Histones; D000097584:DNA Methyltransferase 3B; C000715535:Dnmt3a protein, mouse; C000619381:Dnmt1 protein, mouse; C475961:gamma-H2AX protein, mouse