Effects of chloromethanes on growth of and deletion of the pce gene cluster in dehalorespiring Desulfitobacterium hafniense strain Y51.
The dehalorespiring Desulfitobacterium hafniense strain Y51 efficiently dechlorinates tetrachloroethene (PCE) to cis-1,2-dichloroethene (cis-DCE) via trichloroethene by PceA reductive dehalogenase encoded by the pceA gene. In a previous study, we found that the significant growth inhibition of strain Y51 occurred in the presence of commercial cis-DCE. In this study, it turned out that the growth inhibition was caused by chloroform (CF) contamination of cis-DCE. Interestingly, CF did not affect the growth of PCE-nondechlorinating SD (small deletion) and LD (large deletion) variants, where the former fails to transcribe the pceABC genes caused by a deletion of the promoter and the latter lost the entire pceABCT gene cluster. Therefore, PCE-nondechlorinating variants, mostly LD variant, became predominant, and dechlorination activity was significantly reduced in the presence of CF. Moreover, such a growth inhibitory effect was also observed in the presence of carbon tetrachloride at 1 microM, but not carbon dichloride even at 1 mM.
Futagami
Taiki
T
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
Yamaguchi
Takehito
T
Nakayama
Shun-Ichi
S
Goto
Masatoshi
M
Furukawa
Kensuke
K
D001483:Base Sequence; D001673:Biodegradation, Environmental; D002725:Chloroform / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D045858:Desulfitobacterium / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D004000:Dichloroethylenes / Q000378:metabolism / Q000494:pharmacology; D004785:Environmental Pollutants / Q000378:metabolism / Q000494:pharmacology; D017353:Gene Deletion; D005798:Genes, Bacterial; D014644:Genetic Variation; D008737:Methyl Chloride / Q000378:metabolism / Q000494:pharmacology*; D005810:Multigene Family; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D004000:Dichloroethylenes; D004785:Environmental Pollutants; D002725:Chloroform; D008737:Methyl Chloride; D010088:Oxidoreductases; C098849:tetrachloroethene reductase; C035384:1,2-dichloroethylene