Fluorescence in situ hybridization for rapid identification of Achromobacter xylosoxidans and Alcaligenes faecalis recovered from cystic fibrosis patients.
Achromobacter xylosoxidans is frequently isolated from the respiratory secretions of cystic fibrosis (CF) patients, but identification with biochemical tests is unreliable. We describe fluorescence in situ hybridization assays for the rapid identification of Achromobacter xylosoxidans and Alcaligenes faecalis. Both assays showed high sensitivities and high specificities with a collection of 155 nonfermenters from CF patients.
Wellinghausen
Nele
N
Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert-Koch Str. 8, D-89081 Ulm, Germany. nele.wellinghausen@uniklinik-ulm.de
Wirths
Beate
B
Poppert
Sven
S
D042441:Achromobacter denitrificans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D042481:Alcaligenes faecalis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D003550:Cystic Fibrosis / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors