Inhibitory effects of antioxidant constituents from Melothria heterophylla on matrix metalloproteinase-1 expression in UVA-irradiated human dermal fibroblasts.
Matrix metalloproteinases (MMPs) are known to play an important role in photoaging by mediating the degradation of extracellular matrix proteins. To develop a new anti-aging agent for cosmetics from natural products, Melothria heterophylla (Lour.) Cogn. was selected for its antioxidant activity and inhibitory effect on expression of MMP-1 in UVA-irradiated human skin fibroblasts. Two compounds (compounds 1 and 2 ) were isolated from an ethyl acetate soluble fraction of the ethanolic extracts; they were identified as 1,2,4,6-tetra-O-galloyl-beta-(D)-glucopyranose (1) and 3,4,5-trihydroxybenzoic acid (2). These compounds were found to scavenge radicals and reactive oxygen species (ROS) and were measured to have SC50 values of 3.9 microM and 13.3 microM against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and values of 4.3 microM and 4.0 microM against superoxide radicals in the xanthine/xanthine oxidase system, respectively. Compounds 1 and 2 showed a dose-dependent inhibitory effect on the expression and activity of MMP-1 in UVA-induced human skin fibroblasts, but no inhibition of MMP-1 mRNA expression. Therefore, we concluded that compounds 1 and 2 significantly inhibited MMP-1 expression at the protein level. Also, these compounds were determined to have a potent antioxidant activity. From these results, we suggest that these compounds might be useful as a new anti-aging agent for photodamaged skin, but the in vitro findings must be verified in in vivo studies.
Cho
Y H
YH
R&D Center, Hanbul Cosmetics Corporation, Umsung-Kun, Chungbuk 369-834, Korea.
Kim
J H
JH
Sim
G S
GS
Lee
B C
BC
Pyo
H B
HB
Park
H D
HD
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D019662:Cucurbitaceae / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D005707:Gallic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005947:Glucose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000235:genetics; D061965:Matrix Metalloproteinase Inhibitors; D019906:Nuclear Magnetic Resonance, Biomolecular; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet; D013481:Superoxides / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D006834:Hydrazines; D061965:Matrix Metalloproteinase Inhibitors; D010851:Picrates; D010936:Plant Extracts; D012333:RNA, Messenger; D013481:Superoxides; D005707:Gallic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D020781:Matrix Metalloproteinase 1; D005947:Glucose