title: Complete-genome phylogenetic approach to varicella-zoster virus evolution: genetic divergence and evidence for recombination.
abstract: Recent studies of varicella-zoster virus (VZV) DNA sequence variation, involving large numbers of globally distributed clinical isolates, suggest that this virus has diverged into at least three distinct genotypes designated European (E), Japanese (J), and mosaic (M). In the present study, we determined and analyzed the complete genomic sequences of two M VZV strains and compared them to the sequences of three E strains and two J strains retrieved from GenBank (including the Oka vaccine preparation, V-Oka). Except for a few polymorphic tandem repeat regions, the whole genome, representing approximately 125,000 nucleotides, is highly conserved, presenting a genetic similarity between the E and J genotypes of approximately 99.85%. These analyses revealed that VZV strains distinctly segregate into at least four genotypes (E, J, M1, and M2) in phylogenetic trees supported by high bootstrap values. Separate analyses of informative sites revealed that the tree topology was dependent on the region of the VZV genome used to determine the phylogeny; collectively, these results indicate the observed strain variation is likely to have resulted, at least in part, from interstrain recombination. Recombination analyses suggest that strains belonging to the M1 and M2 genotypes are mosaic recombinant strains that originated from ancestral isolates belonging to the E and J genotypes through recombination on multiple occasions. Furthermore, evidence of more recent recombination events between M1 and M2 strains is present in six segments of the VZV genome. As such, interstrain recombination in dually infected cells seems to figure prominently in the evolutionary history of VZV, a feature it has in common with other herpesviruses. In addition, we report here six novel genomic targets located in open reading frames 51 to 58 suitable for genotyping of clinical VZV isolates.
authors.affiliation: Department of Clinical Virology, Göteborg University, Guldhedsgatan 10b, 413 46 Göteborg, Sweden. peter.norberg@microbio.gu.se
authors.forename: Peter
authors.initials: P
authors.lastname: Norberg
authors.forename: Jan-Ake
authors.initials: JA
authors.lastname: Liljeqvist
authors.forename: Tomas
authors.initials: T
authors.lastname: Bergström
authors.forename: Scott
authors.initials: S
authors.lastname: Sammons
authors.forename: D Scott
authors.initials: DS
authors.lastname: Schmid
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Loparev
mesh_terms: D001483:Base Sequence; D016679:Genome, Viral / Q000235:genetics*; D014645:Herpesvirus 3, Human / Q000235:genetics*; D010802:Phylogeny; D011995:Recombination, Genetic / Q000235:genetics*