title: Multi-tasking of nonstructural gene products is required for bean yellow dwarf geminivirus transcriptional regulation.
abstract: Mastreviridae, of the family geminiviridae, possess a monopartite genome and are transmitted by leafhoppers. Bean yellow dwarf dirus (BeYDV) is a mastrevirus which originated from South Africa and infects dicoyledenous plants, a feature unusual for mastreviridae. Previously, the nonstructural proteins Rep and RepA were examined with respect to their independent roles in BeYDV replication. This was achieved by placing both gene products under independent constitutive promoter control and examining their effects on replication-competent constructs. In the current study, Rep and RepA are examined further for their roles in regulating BeYDV gene expression using a series of replication-incompetent constructs. While both Rep and RepA are found to behave as equally potent inhibitors of complementary-sense gene expression, they differ considerably with respect to their abilities to transactivate virion-sense gene expression. Furthermore, RepA is identified as playing more than one role in this transactivation process. A nuclear localization domain is identified in Rep which is absent in RepA, and Rep-RepA interactions are examined under in vivo conditions. The study concludes with an investigation into the expression strategies of the BeYDV capsid protein.
authors.affiliation: Cornell University, Cornell Research Foundation, Ithaca, NY, USA. klh22@cornell.edu
authors.forename: Kathleen L
authors.initials: KL
authors.lastname: Hefferon
authors.forename: Yong-Sun
authors.initials: YS
authors.lastname: Moon
authors.forename: Ying
authors.initials: Y
authors.lastname: Fan
mesh_terms: D007887:Fabaceae / Q000821:virology*; D017791:Geminiviridae / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D017361:Viral Nonstructural Proteins / Q000502:physiology*
chemical_list: D017361:Viral Nonstructural Proteins