Temporal and topographic profiles of tissue hypoxia following transient focal cerebral ischemia in rats.
Intravascular accumulation of blood cells after brain ischemia-reperfusion can cause obstruction of cerebral blood flow and tissue hypoxia/ischemia as a consequence. In the present study, we examined temporal and topographic changes of tissue hypoxia/ischemia after occlusion of the middle cerebral artery (MCA) for 60 min in rats with immunohistochemical staining for hypoxia (2-nitroimidazole hypoxia marker: hypoxyprobe-1 adducts). Our results showed that tissue hypoxia expressed as positive staining for hypoxyprobe-1 adducts preceded neuronal degeneration. Platelets and granulocytes were detected close to the hypoxyprobe-1 adducts positive area. These results suggested that the hypoxic environment could persist even after reperfusion of MCA, because of vascular obstruction with accumulation of platelets and granulocytes.
Noto
Takahisa
T
Medicinal Biology Research Laboratories, Astellas Pharmaceutical Inc, Osaka, Japan.
Furuichi
Yasuhisa
Y
Ishiye
Masayuki
M
Matsuoka
Nobuya
N
Aramori
Ichiro
I
Mutoh
Seitaro
S
Yanagihara
Takehiko
T
Manabe
Noboru
N
D000818:Animals; D001921:Brain / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D002545:Brain Ischemia / Q000503:physiopathology*; D015971:Gene Expression Regulation, Enzymologic; D039661:Integrin beta3 / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology*; D013997:Time Factors
D039661:Integrin beta3; D009195:Peroxidase