title: Dendrite-selective redistribution of the chemokine receptor CXCR4 following agonist stimulation.
abstract: The chemokine SDF-1 is a secreted protein that plays a critical role in several aspects of neuron development through interaction with its unique receptor CXCR4. A key mechanism that controls neuron responsiveness to extracellular signals during neuronal growth is receptor endocytosis. Since we previously reported that SDF-1 regulates axon development without affecting the other neurites, we asked whether this could correlate with a compartment-selective trafficking of CXCR4. We thus studied CXCR4 behavior upon SDF-1 exposure in rat hippocampus slices and in transfected neuron cultures. A massive agonist-induced redistribution of CXCR4 in endosomes was observed in dendrites whereas no modification was evidenced in axons. Our data suggest that CXCR4 trafficking may play a role in mediating selective effects of SDF-1 on distinct neuronal membrane subdomains.
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale, I.N.S.E.R.M., Unité 643, I.T.E.R.T, CHU Hotel-Dieu, University of Nantes, 30 Bd Jean Monnet, 44035 Nantes Cedex 01, France.
authors.forename: Stéphane J
authors.initials: SJ
authors.lastname: Baudouin
authors.forename: Fabien
authors.initials: F
authors.lastname: Pujol
authors.forename: Arnaud
authors.initials: A
authors.lastname: Nicot
authors.forename: Patrick
authors.initials: P
authors.lastname: Kitabgi
authors.forename: Hélène
authors.initials: H
authors.lastname: Boudin
mesh_terms: D000818:Animals; D019390:Arrestins / Q000378:metabolism; D001369:Axons / Q000378:metabolism; D002451:Cell Compartmentation / Q000502:physiology; D002478:Cells, Cultured; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000494:pharmacology; D003712:Dendrites / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology; D011992:Endosomes / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006624:Hippocampus / Q000166:cytology; D008297:Male; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019718:Receptors, CXCR4 / Q000819:agonists* / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D000071557:beta-Arrestins
chemical_list: D019390:Arrestins; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; C486264:Cxcr4 protein, rat; D019718:Receptors, CXCR4; D000071557:beta-Arrestins; D049452:Green Fluorescent Proteins