title: Self-assembly of receptor/signaling complexes in bacterial chemotaxis.
abstract: Escherichia coli chemotaxis is mediated by membrane receptor/histidine kinase signaling complexes. Fusing the cytoplasmic domain of the aspartate receptor, Tar, to a leucine zipper dimerization domain produces a hybrid, lzTar(C), that forms soluble complexes with CheA and CheW. The three-dimensional reconstruction of these complexes was different from that anticipated based solely on structures of the isolated components. We found that analogous complexes self-assembled with a monomeric cytoplasmic domain fragment of the serine receptor without the leucine zipper dimerization domain. These complexes have essentially the same size, composition, and architecture as those formed from lzTar(C). Thus, the organization of these receptor/signaling complexes is determined by conserved interactions between the constituent chemotaxis proteins and may represent the active form in vivo. To understand this structure in its cellular context, we propose a model involving parallel membrane segments in receptor-mediated CheA activation in vivo.
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Wolanin
authors.forename: Melinda D
authors.initials: MD
authors.lastname: Baker
authors.forename: Noreen R
authors.initials: NR
authors.lastname: Francis
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Thomas
authors.forename: David J
authors.initials: DJ
authors.lastname: DeRosier
authors.forename: Jeffry B
authors.initials: JB
authors.lastname: Stock
mesh_terms: D002633:Chemotaxis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D017459:Receptors, Amino Acid / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D012542:Scattering, Radiation; D015398:Signal Transduction; D012995:Solubility
chemical_list: D046912:Multiprotein Complexes; D017459:Receptors, Amino Acid; C031959:aspartic acid receptor; C038039:serine receptor