title: Regeneration of osteonecrosis of canine scapho-lunate using bone marrow stromal cells: possible therapeutic approach for Kienböck disease.
abstract: We evaluated the ability of canine bone marrow stromal cells (cBMSCs) to regenerate bone in a cavity of the scapholunate created by curretage and freeze-thawing with liquid nitrogen (LN). Autologous BMSCs were harvested from the iliac crest and expanded in vitro. Their potential to differentiate into osteo-, chondro-, and adipogenic lineages was confirmed using a standard differentiation induction assay. LN-treated scapholunates showed no regeneration of bone tissue when the cavity was left alone, demonstrating severe collapse and deformity as observed in human Kienböck disease. A combination of beta-tri-calcium phosphate and a vascularized bone graft with autologous fibroblasts failed to regenerate bone in the LN-treated cavity. When the same procedure was performed using BMSCs, however, LN-treated scapholunates showed no collapse and deformity, and the cavity was completely filled with normal cancerous bone within 4 weeks. These results suggested the potential of using BMSCs to treat Kienböck disease.
authors.affiliation: Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Ikeguchi
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kakinoki
authors.forename: Tomoki
authors.initials: T
authors.lastname: Aoyama
authors.forename: Kotaro Roberts
authors.initials: KR
authors.lastname: Shibata
authors.forename: Seiji
authors.initials: S
authors.lastname: Otsuka
authors.forename: Kennichi
authors.initials: K
authors.lastname: Fukiage
authors.forename: Koichi
authors.initials: K
authors.lastname: Nishijo
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ishibe
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Shima
authors.forename: Bungo
authors.initials: B
authors.lastname: Otsuki
authors.forename: Takashi
authors.initials: T
authors.lastname: Azuma
authors.forename: Sadami
authors.initials: S
authors.lastname: Tsutsumi
authors.forename: Tomitaka
authors.initials: T
authors.lastname: Nakayama
authors.forename: Takanobu
authors.initials: T
authors.lastname: Otsuka
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Junya
authors.initials: J
authors.lastname: Toguchida
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D016026:Bone Marrow Transplantation / Q000379:methods*; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D002130:Calcium Phosphates / Q000627:therapeutic use; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000187:drug effects / Q000502:physiology; D004285:Dogs; D012667:Lunate Bone / Q000187:drug effects / Q000502:physiology / Q000601:surgery*; D008279:Magnetic Resonance Imaging / Q000379:methods; D009584:Nitrogen / Q000627:therapeutic use; D010007:Osteochondritis / Q000628:therapy; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D010020:Osteonecrosis / Q000000981:diagnostic imaging / Q000503:physiopathology / Q000628:therapy*; D011859:Radiography; D017154:Stromal Cells / Q000166:cytology* / Q000637:transplantation; D014182:Transplantation, Autologous
chemical_list: D002130:Calcium Phosphates; C020243:calcium phosphate; D009584:Nitrogen