title: Microinjection of galanin-like peptide into the medial preoptic area stimulates food intake in adult male rats.
abstract: Galanin-like peptide (GALP) is a neuropeptide implicated in the regulation of feeding behaviour, metabolism and reproduction. GALP is an endogenous ligand of the galanin receptors, which are widely expressed in the hypothalamus. GALP is predominantly expressed in arcuate nucleus (ARC) neurones, which project to the paraventricular nucleus (PVN) and medial preoptic area (mPOA). Intracerebroventricular or intraparaventricular (iPVN) injection of GALP acutely increases food intake in rats. The effect of GALP injection into the mPOA on feeding behaviour has not previously been studied. In the present study, intra-mPOA (imPOA) injection of GALP potently increased 0-1-h food intake in rats. The dose-response effect of imPOA GALP administration on food intake was similar to that previously observed following iPVN administration. The effects of GALP (1 nmol) or galanin (1 nmol) on food intake were then compared following injection into the PVN, mPOA, ARC, dorsal medial nucleus (DMN), lateral hypothalamus and rostral preoptic area (rPOA). GALP (1 nmol) increased food intake to a similar degree when injected into the imPOA or iPVN, but produced no significant effect when injected into the ARC, DMN, lateral hypothalamus or rPOA. Similarly, galanin (1 nmol) significantly increased food intake following injection imPOA and iPVN. However, the effect was significantly smaller than that following administration of GALP (1 nmol). Galanin also had no significant effect on food intake when administered into the ARC, DMN, lateral hypothalamus and rPOA. These data suggest that the mPOA and the PVN may have specific roles in mediating the orexigenic effect of GALP and galanin.
authors.affiliation: Department of Metabolic Medicine, Imperial College London, Hammersmith Hospital, London, UK.
authors.forename: M
authors.initials: M
authors.lastname: Patterson
authors.forename: K G
authors.initials: KG
authors.lastname: Murphy
authors.forename: E L
authors.initials: EL
authors.lastname: Thompson
authors.forename: K L
authors.initials: KL
authors.lastname: Smith
authors.forename: K
authors.initials: K
authors.lastname: Meeran
authors.forename: M A
authors.initials: MA
authors.lastname: Ghatei
authors.forename: S R
authors.initials: SR
authors.lastname: Bloom
mesh_terms: D000818:Animals; D001931:Brain Mapping; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects*; D019004:Galanin / Q000008:administration & dosage / Q000494:pharmacology; D044089:Galanin-Like Peptide / Q000008:administration & dosage / Q000494:pharmacology*; D007031:Hypothalamus / Q000033:anatomy & histology / Q000502:physiology; D008297:Male; D008845:Microinjections; D011301:Preoptic Area / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical; D013552:Swine
chemical_list: D044089:Galanin-Like Peptide; D019004:Galanin