Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation.
Mushroom bodies (MBs) are the centers for olfactory associative learning and elementary cognitive functions in the Drosophila brain. As a way to systematically elucidate genes preferentially expressed in MBs, we have analyzed genome-wide alterations in transcript profiles associated with MB ablation by hydroxyurea. We selected 100 genes based on microarray data and examined their expression patterns in the brain by in situ hybridization. Seventy genes were found to be expressed in the posterodorsal cortex, which harbors the MB cell bodies. These genes encode proteins of diverse functions, including transcription, signaling, cell adhesion, channels, and transporters. Moreover, we have examined developmental functions of 40 of the microarray-identified genes by transgenic RNA interference; 8 genes were found to cause mild-to-strong MB defects when suppressed with a MB-Gal4 driver. These results provide important information not only on the repertoire of genes that control MB development but also on the repertoire of neural factors that may have important physiological functions in MB plasticity.
Kobayashi
Masatomo
M
Graduate School of Life and Environmental Sciences and Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba 305-8572, Japan.
Michaut
Lydia
L
Ino
Ayako
A
Honjo
Ken
K
Nakajima
Taiki
T
Maruyama
Yasushi
Y
Mochizuki
Hiroaki
H
Ando
Mai
M
Ghangrekar
Indrayani
I
Takahashi
Kuniaki
K
Saigo
Kaoru
K
Ueda
Ryu
R
Gehring
Walter J
WJ
Furukubo-Tokunaga
Katsuo
K
D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect / Q000235:genetics; D006918:Hydroxyurea / Q000494:pharmacology*; D046228:Microarray Analysis / Q000379:methods*; D024521:Mushroom Bodies / Q000002:abnormalities / Q000166:cytology / Q000187:drug effects*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics*
D012333:RNA, Messenger; D006918:Hydroxyurea