title: Quantification by image analysis of the Gallus gallus lung elastic fibres from embryonic to adult birds.
abstract: The organization of the lung's elastic fibres is amazingly uniform in all vertebrates, with the possible exception of birds, whose pulmonary architecture and air movement are unique. The overall goal of this work was to study and quantify elastic fibre distribution patterns and relative amounts in the parabronchi, during the incubation period until the 42nd day after hatching. Chick embryo lungs were examined on the 14th, 16th, 18th and 20th days of incubation and chick lungs on the 1st, 2nd, 7th, 14th, 35th and 42nd days after hatching. Four animals were used daily, and the observations were randomly performed on both lungs. A morphometric study was carried out focusing on the computerized image analysis of histological sections stained according to a modified Gomori technique. The values obtained for each day result from the observation and processing of 20 images. Complementary studies were performed using transmission electron microscopy, as on the 14th embryonic day the fibres were not visible on light microscopy. The results show that the area occupied by the elastic fibres increases gradually from the 16th day of incubation up till the 7th day after hatching and decreases slowly in the following days of the study. A prominent increase takes place before hatching, which points out to the adequate and essential structural roles played by the elastic fibres in the pulmonary maturation process.
authors.affiliation: Department of Pathology and Veterinary Clinics, Centre for Studies on Agricultural and Veterinary Sciences, University of Trás-os-Montes e Alto Douro, 5001-911 Vila Real, Portugal. lpinto@utad.pt
authors.forename: M
authors.initials: M
authors.lastname: de Lurdes Pinto
authors.forename: C
authors.initials: C
authors.lastname: Gonçalves
authors.forename: P
authors.initials: P
authors.lastname: Rodrigues
authors.forename: V A
authors.initials: VA
authors.lastname: Bairos
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D002196:Capillaries / Q000648:ultrastructure; D002642:Chick Embryo / Q000648:ultrastructure*; D002645:Chickens; D004547:Elastic Tissue / Q000648:ultrastructure*; D008168:Lung / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission / Q000379:methods / Q000662:veterinary; D011650:Pulmonary Alveoli / Q000648:ultrastructure*