title: A self-contained polymeric 2-DE chip system for rapid and easy analysis.
abstract: We developed a polymeric 2-DE chip system. The chip consisted of an IEF region, an SDS-PAGE region, a valveless connection port, and a sample introduction port. A "junction structure" as a valveless connection port, which allowed separating and connecting the first- and second-dimensional gels, was fabricated between their regions. A "solution inlet" as a sample introduction port was fabricated to perform the liquid and sample introductions without solution leakage. Simultaneous sample monitoring was performed using the on-chip detection system. The performances of the system were demonstrated using commercially available proteins as a standard specimen and tissue-extracted proteins as the real samples. All procedures were employed without any movement of relocation part. This new 2-D separation system realized improved labor-intensive operations and a reduced experimental time.
authors.affiliation: Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Usui
authors.forename: Atsunori
authors.initials: A
authors.lastname: Hiratsuka
authors.forename: Kisho
authors.initials: K
authors.lastname: Shiseki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Maruo
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Matsushima
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Takahashi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Unuma
authors.forename: Koji
authors.initials: K
authors.lastname: Sakairi
authors.forename: Ichiji
authors.initials: I
authors.lastname: Namatame
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D011108:Polymers; D011506:Proteins