title: IndexToolkit: an open source toolbox to index protein databases for high-throughput proteomics.
abstract: A software package, IndexToolkit, aimed at overcoming the disadvantage of FASTA-format databases for frequent searching, is developed to utilize an indexing strategy to substantially accelerate sequence queries. IndexToolkit includes user-friendly tools and an Application Programming Interface (API) to facilitate indexing, storage and retrieval of protein sequence databases. As open source, it provides a sequence-retrieval developing framework, which is easily extensible for high-speed-request proteomic applications, such as database searching or modification discovering. We applied IndexToolkit to database searching engine pFind to demonstrate its effect. Experimental studies show that IndexToolkit is able to support significantly faster searches of protein database.
  
  
  BACKGROUND
  The IndexToolkit is free to use under the open source GNU GPL license. The source code and the compiled binary can be freely accessed through the website http://pfind.jdl.ac.cn/IndexToolkit. In this website, the more detailed information including screenshots and documentations for users and developers is also available.
authors.affiliation: Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China. dqli@jdl.ac.cn
authors.forename: Dequan
authors.initials: D
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Gao
authors.forename: Charles X
authors.initials: CX
authors.lastname: Ling
authors.forename: Xiaobiao
authors.initials: X
authors.lastname: Wang
authors.forename: Ruixiang
authors.initials: R
authors.lastname: Sun
authors.forename: Simin
authors.initials: S
authors.lastname: He
mesh_terms: D003628:Database Management Systems; D030562:Databases, Protein; D020869:Gene Expression Profiling / Q000379:methods; D016247:Information Storage and Retrieval / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein / Q000379:methods*; D012984:Software; D014584:User-Computer Interface