title: The effect of hyaluronan combined with KI3 complex (Hyiodine wound dressing) on keratinocytes and immune cells.
abstract: Hyiodine (high molecular weight hyaluronan combined with KI3 complex) is a new non-adhesive wound dressing which significantly improves the healing process. The aim of the study was to investigate the effects of Hyoidine on functional properties of isolated human keratinocytes and leukocytes, and on those of U937 and HL60 cell lines. While KI3 complex inhibited the viability and proliferation of the cells tested, Hyiodine did not have any significant effect. The expression of CD11b, CD62L and CD69 on PMNL, monocytes and lymphocytes, as well as the oxidative burst of blood neutrophils, were not changed. On the contrary, Hyiodine inhibited the PMA-activated oxidative burst and significantly increased the production of IL-6 and TNF-alpha by lymphocytes. It was concluded that hyaluronan content of Hyiodine reduces the toxic effect of KI3 complex on cells and speeds up the wound healing process by increasing the production of inflammatory cytokines.
authors.affiliation: CPN spol. s r o, Dolni Dobrouc 401, 562 01, Dolni Dobrouc, Czech Republic. frankova@contipro.cz
authors.forename: Jana
authors.initials: J
authors.lastname: Frankova
authors.forename: Lukas
authors.initials: L
authors.lastname: Kubala
authors.forename: Vladimir
authors.initials: V
authors.lastname: Velebny
authors.forename: Milan
authors.initials: M
authors.lastname: Ciz
authors.forename: Antonin
authors.initials: A
authors.lastname: Lojek
mesh_terms: D018922:HL-60 Cells; D006490:Hemostatics / Q000494:pharmacology; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007107:Immune System / Q000166:cytology / Q000187:drug effects*; D007454:Iodides / Q000494:pharmacology; D007455:Iodine / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects*; D020298:U937 Cells
chemical_list: D006490:Hemostatics; D007454:Iodides; C512827:hyaluronan-iodine complex; D006820:Hyaluronic Acid; D007455:Iodine; C010389:Lugol's solution