Leukocyte-endothelium interaction: measurement by laser tweezers force spectroscopy.
Leukocyte adhesion to vascular endothelium is an initial step of many inflammatory diseases. Although the atomic force microscopy (AFM) measurements of leukocyte-endothelial interaction have been recently introduced. with cell adhesion force unbinding curves (CAFUC). We obtained pico-Newton force in the initial interaction between a single living THP-1 cell and HUVEC monolayer using a custom-built laser tweezers (LT) system. The measured quantities included the non-linear force-distance relationship, and the effect of yielding in cell detachment. It is possible to introduce a time scale into the LT cell-detachment experiments for further exploration and more detailed information on the viscoelastic properties of living cells.
Wang
Shi-Kang
SK
Mechanical Engineering College, Tianjin University, Tianjin 300072, China.
Chiu
Jeng-Jiann
JJ
Lee
Ming-Rou
MR
Chou
Shih-Chin
SC
Chen
Li-Jing
LJ
Hwang
Ned H C
NH
D000268:Adhesiveness; D002448:Cell Adhesion / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007834:Lasers; D007962:Leukocytes / Q000166:cytology / Q000502:physiology*; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods; D013314:Stress, Mechanical