title: Canine hemophagocytic histiocytic sarcoma: a proliferative disorder of CD11d+ macrophages.
abstract: Histiocytic disorders of dogs include histiocytoma, localized histiocytic sarcoma (HS), disseminated HS (malignant histocytosis), and the reactive histiocytoses: cutaneous and systemic. A common element to these diseases is proliferation of dendritic cells (DC) of either Langerhans cell (epithelial DC) or interstitial DC lineage. In this report, 17 dogs with hemophagocytic HS are described. Breeds affected included Bernese Mountain Dog (6), Golden Retriever (4), Rottweiler (3), Labrador Retriever (2), a mixed-breed dog, and a Schnauzer, which were from 2.5 to 13 years old. The dogs presented with Coombs negative responsive anemia in 16/17 dogs (94%), thrombocytopenia in 15/17 dogs (88%), hypoalbuminemia in 16/17 dogs (94%), and hypocholesterolemia in 11/16 dogs (69%). All dogs died or were euthanized. The clinical course ranged from 2 to 32 weeks (mean 7.1 weeks). Diffuse splenomegaly with ill-defined masses was consistently present. Microscopic lesions were prevalent in spleen, liver, lung, and bone marrow. Metastasis occurred by insidious intravascular invasion with minimal mass formation. Histiocytes were markedly erythrophagocytic and accompanied by foci of extramedullary hemopoiesis. Cytologically, the histiocytes varied from well differentiated to atypical, with atypia more prevalent in spleen than bone marrow. These tumors arose from splenic red pulp and bone marrow macrophages, which expressed major histocompatibility complex class II and the beta2 integrin, CD11d. They had low and/or inconsistent expression of CD1 and CD11c, which are dominantly expressed by canine nonhemophagocytic HS of DC origin. Canine histiocytic proliferative diseases now encompass proliferation of all members of the myeloid histiocytic lineage: Langerhans cells, interstitial DC, and macrophages.
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California at Davis, Davis, CA 95616, USA. pfmoore@ucdavis.edu
authors.forename: P F
authors.initials: PF
authors.lastname: Moore
authors.forename: V K
authors.initials: VK
authors.lastname: Affolter
authors.forename: W
authors.initials: W
authors.lastname: Vernau
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D018845:CD11 Antigens / Q000378:metabolism*; D004283:Dog Diseases / Q000175:diagnosis* / Q000473:pathology; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015620:Histiocytic Disorders, Malignant / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D016023:Integrins / Q000378:metabolism*; D008099:Liver / Q000473:pathology; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D012509:Sarcoma / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D013154:Spleen / Q000473:pathology
chemical_list: D018845:CD11 Antigens; D016023:Integrins