In vivo insertional mutagenesis in Corynebacterium pseudotuberculosis: an efficient means to identify DNA sequences encoding exported proteins.
The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport subunits and for hypothetical and unknown-function proteins.
Dorella
Fernanda A
FA
Laboratório de Genética Celular e Molecular, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Estevam
Estela M
EM
Pacheco
Luis G C
LG
Guimarães
Cláudia T
CT
Lana
Ubiraci G P
UG
Gomes
Eliane A
EA
Barsante
Michele M
MM
Oliveira
Sérgio C
SC
Meyer
Roberto
R
Miyoshi
Anderson
A
Azevedo
Vasco
V
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport; D016925:Corynebacterium pseudotuberculosis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004251:DNA Transposable Elements; D013293:Enterococcus faecalis / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D000469:Alkaline Phosphatase