The automatic production of 16alpha-[18F]fluoroestradiol using a conventional [18F]FDG module with a disposable cassette system.
We have developed a fully automatic method for the synthesis of 16alpha-[18F]fluoroestradiol ([18F]FES) using a disposable cassette system and conventional [18F]FDG module. [18F]FES was synthesized using a GE TracerLab MX module and a modified module control program. Following [18F]fluorination, we hydrolyzed the product three times with a mixture of 2N HCl and CH(3)CN. After HPLC purification, the decay corrected radiochemical yield of [18F]FES was 45.3+/-2.8%, which was stable to 98.2+/-0.2% at 6h after synthesis. This new automated synthesis method provides high and reproducible yields with the advantage of a disposable cassette system.
Oh
Seung Jun
SJ
Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, 388-1 Pungnap-dong, Songpa-gu, Seoul 138-736, Korea. sjoh@amc.seoul.kr
Chi
Dae Yoon
DY
Mosdzianowski
Christoph
C
Kil
Hee Seup
HS
Ryu
Jin Sook
JS
Moon
Dae Hyuk
DH
D001943:Breast Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004958:Estradiol / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D005260:Female; D005462:Fluorine Radioisotopes / Q000737:chemistry*; D019788:Fluorodeoxyglucose F18 / Q000737:chemistry; D006801:Humans; D049268:Positron-Emission Tomography; D011786:Quality Control; D011854:Radiochemistry / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D011960:Receptors, Estrogen / Q000378:metabolism
D005462:Fluorine Radioisotopes; D019275:Radiopharmaceuticals; D011960:Receptors, Estrogen; D019788:Fluorodeoxyglucose F18; D004958:Estradiol; C043436:16-fluoroestradiol