[Expression of Ang2 and Tie2 and their relation with the angiogenesis of hepatocellular carcinoma in rats].
OBJECTIVE
To investigate the relationship between the expression of Ang2, Tie2 and the angiogenesis of hepatocellular carcinoma in rats.


METHODS
Thirty-eight healthy male rats were randomly divided into 3 groups: 5 rats in the control group; 25 rats in the experimental group were equally divided into 5-day, 10-day, 15-day, 20-day, and 25-day groups; the other 8 rats were used as the supplement of the experimental group. An allogenic transplanted rat model of CBRH-7919 hepatocellular carcinoma in situ was established by immunosuppression. The expressions of Ang2 and Tie2 were detected by immunohistochemical staining in cancerous tissues of different developmental stages and liver tissues of the control group. At the same time, microvessel density was determined by anti-CD31 immunohistochemical staining.


RESULTS
CBRH-7919 hepatocellular carcinoma models were successfully set up in 24 rats. The expression level of Ang2 and Tie2 in cancerous tissues was much higher than that of liver tissues of the control group (P <0.05). The overexpression of Ang2 was pristine and continuous in different developmental stages. The expressions of Ang2 and Tie2 positively correlated with microvessal density in hepatocellular carcinoma (P<0.05).


CONCLUSIONS
The up-regulation of Ang2 and Tie2 may play important roles in the angiogenesis of hepatocellular carcinoma. Ang2 may participate in the start of angiogenesis of hepatocellular carcinoma.
Li
Wen-zheng
WZ
Department of Radiology, Xiangya Hospital, Central South University, Changsha 410008, China. wenzheng727@163.com
Yang
Pu
P
Peng
Guang-chun
GC
Tang
Hui-huan
HH
Zhou
Jun
J
Fu
Chun-yan
CY
Feng
De-yun
DY
Zhou
Xuan
X
Huang
Man-ping
MP
Zhou
Gao-feng
GF
D042702:Angiopoietin-2 / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D008114:Liver Neoplasms, Experimental / Q000098:blood supply* / Q000378:metabolism*; D008297:Male; D009389:Neovascularization, Pathologic; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D042787:Receptor, TIE-2 / Q000096:biosynthesis* / Q000235:genetics
D042702:Angiopoietin-2; D012333:RNA, Messenger; D042787:Receptor, TIE-2