title: Performance of expression vector, pTD1, in insect cell-free translation system.
abstract: We constructed a pTD1 vector for an insect cell-free translation system containing a 5' untranslated region (UTR) of a polyhedrin gene as a translational enhancer sequence. Its translational efficiency was about 50-fold higher than those of mRNAs without an enhancer sequence. Moreover, the pTD1 vector functioned as an effective expression vector not only in the insect cell-free translation system but also in wheat germ extract and rabbit reticulocyte lysate systems.
authors.affiliation: Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation, Kyoto 604-8511, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ito
authors.forename: Toru
authors.initials: T
authors.lastname: Ezure
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Shikata
authors.forename: Koji
authors.initials: K
authors.lastname: Tanimizu
authors.forename: Osamu
authors.initials: O
authors.lastname: Nishimura
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002474:Cell-Free System; D005786:Gene Expression Regulation; D024861:Genetic Enhancement / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D000077183:Occlusion Body Matrix Proteins; D014176:Protein Biosynthesis / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D018411:Spodoptera / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection / Q000379:methods; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*; D015678:Viral Structural Proteins
chemical_list: D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus