title: Phagocytosis, production of nitric oxide and pro-inflammatory cytokines by macrophages in the presence of dematiaceous [correction of dematiaceus] fungi that cause chromoblastomycosis.
abstract: Chromoblastomycosis is characterized by the slow development of polymorphic skin lesions (nodules, verrucas, tumores, plaques and scar tissue). Inside the host, infectious propagules adhere to epithelial cells and differentiate into sclerotic forms, which effectively resist destruction by host effector cells and allow onset of chronic disease. A cellular immune response against fungi is essential to control infection. Amongst the cells of the immune system, macrophages play the most important role in controlling fungal growth. In this study, we show that the fungicidal characteristic of macrophages is dependent on the fungal species that causes chromoblastomycosis. We began by observing that the phagocytic index was higher for Fonsecaea pedrosoi and Rhinocladiella aquaspersa compared with that of other fungi. Complement-mediated phagocytosis was more important for Phialophora verrucosa and R. aquaspersa and was inhibited by mannan when F. pedrosoi and R. aquaspersa conidia were phagocytosed by macrophages. We showed that macrophages killed significantly only R. aquaspersa. We also found that the phagocytosis of fungi has functional consequences for macrophages as phagocytosis resulted in down-modulation of MHC-II and CD80 expression as well as in the inhibition of the basal liberation of NO. However, the inhibition of the basal liberation of NO nor the down-modulation of MHC and co-stimulatory molecules were observed in the presence of R. aquaspersa.
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Hayakawa
authors.forename: E E B
authors.initials: EE
authors.lastname: Ghosn
authors.forename: M
authors.initials: M
authors.lastname: da Gloria Teixeria de Sousa
authors.forename: K S
authors.initials: KS
authors.lastname: Ferreira
authors.forename: S R
authors.initials: SR
authors.lastname: Almeida
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D002478:Cells, Cultured; D002862:Chromoblastomycosis / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D016207:Cytokines / Q000096:biosynthesis* / Q000502:physiology; D005093:Exophiala / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism* / Q000502:physiology; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis*; D010587:Phagocytosis / Q000276:immunology*; D010676:Phialophora / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification; D013045:Species Specificity; D014774:Virulence
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D009569:Nitric Oxide