Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system.
Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi-enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxide has been detected amperometrically. The linear concentration ranges are of pmol/L to nmol/L magnitude, with very low limits of detection. The proposed SIA system can be utilized reliably for the on-line simultaneous detection of creatine and creatinine in pharmaceutical products, as well as in serum samples, with a rate of 34 samples per hour and RSD values better than 0.16% (n=10).
Stefan-van Staden
Raluca-Ioana
RI
Faculty of Chemistry, University of Bucharest, Bucharest, Romania. iustinavanstaden@yahoo.com
Bokretsion
Rahel Girmai
RG
van Staden
Jacobus F
JF
Aboul-Enein
Hassan Y
HY
D000581:Amidohydrolases / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry; D003401:Creatine / Q000032:analysis* / Q000097:blood; D003404:Creatinine / Q000032:analysis* / Q000097:blood; D004800:Enzymes, Immobilized / Q000737:chemistry; D017022:Flow Injection Analysis / Q000295:instrumentation*; D006861:Hydrogen Peroxide / Q000737:chemistry; D009862:Online Systems / Q000295:instrumentation*; D050879:Sarcosine Oxidase / Q000737:chemistry; D014512:Ureohydrolases / Q000737:chemistry
D004800:Enzymes, Immobilized; D002244:Carbon; D003404:Creatinine; D006861:Hydrogen Peroxide; D050879:Sarcosine Oxidase; D000581:Amidohydrolases; C019946:creatininase; D014512:Ureohydrolases; C019944:creatinase; D003401:Creatine