title: Effect of leptin on hypothalamic gene expression in calorie-restricted rats.
abstract: Diminished leptin signaling to the arcuate nucleus of hypothalamus (ARH) may induce calorie restriction (CR)-specific neuroendocrine and metabolic adaptation, which is potentially relevant to the effect of CR. The present study investigated whether restoration of leptin signaling to the ARH could reverse CR-induced alterations in neuropeptide gene expression in rats. Male F344 rats, fed ad libitum or a 30% CR diet from 6 weeks of age, received leptin or vehicle intracerebroventricularly for 14 days via osmotic mini-pumps implanted in the subcutis at 34 weeks of age. The messenger RNA levels were quantified by real-time reverse transcription-polymerase chain reaction using total RNA extracted from microdissected tissues containing the ARH. The results indicated that leptin administration reversed the upregulated expression of neuropeptide Y and agouti-related protein genes in CR rats, suggesting the possibility of a role for the leptin-ARH pathway in the effect of CR.
authors.affiliation: Department of Pathology & Gerontology, Nagasaki University Graduate School of Biomedical Science, 1-12-4 Sakamoto, Nagasaki City 852-8523, Japan.
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Komatsu
authors.forename: Takuya
authors.initials: T
authors.lastname: Chiba
authors.forename: Haruyoshi
authors.initials: H
authors.lastname: Yamaza
authors.forename: Kazuo
authors.initials: K
authors.lastname: To
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Toyama
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Higami
authors.forename: Isao
authors.initials: I
authors.lastname: Shimokawa
mesh_terms: D054366:Agouti Signaling Protein; D054369:Agouti-Related Protein; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism*; D001786:Blood Glucose / Q000032:analysis; D031204:Caloric Restriction; D004435:Eating / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D054439:Ghrelin; D007328:Insulin / Q000097:blood; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D020738:Leptin / Q000097:blood / Q000494:pharmacology*; D008297:Male; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D036361:Peptide Hormones / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: C515197:AGRP protein, rat; D054366:Agouti Signaling Protein; D054369:Agouti-Related Protein; D001786:Blood Glucose; D054439:Ghrelin; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D020738:Leptin; D009478:Neuropeptide Y; D036361:Peptide Hormones; D012333:RNA, Messenger; C422650:ghrelin, des-n-octanoyl