title: Interleukin-10 (IL-10) gene polymorphism as a potential host susceptibility factor in tuberculosis.
abstract: Several genes encoding for different cytokines may play crucial roles in host susceptibility to tuberculosis (TB), since the cytokine production capacity varies among individuals and depends on the cytokine gene polymorphism. The association of the cytokine gene polymorphisms with the development of TB was investigated in this study. DNA samples were obtained from a Turkish population of 81 patients with the different clinical forms of TB, and 50 healthy control subjects. All genotyping (IL-6, IL-10, IFN-gamma, TGF-beta and TNF-alpha) experiments were performed using sequence-specific primers PCR (PCR-SSP). Analysis of allele frequencies showed that IL-10 -1082 G allele frequency was significantly more common in TB patients than healthy controls (37.7% vs 23.0%, p: 0.014). No statistically significant differences were observed between the different clinical forms of the disease. These results suggest that the polymorphisms in IL-10 gene may affect susceptibility to TB and increase risk of developing the disease. To confirm the biological significance of our results, further studies should be performed on other population groups.
authors.affiliation: Microbiology and Infectious Diseases Department, Faculty of Medicine, Uludağ University, 16059 Bursa, Turkey. oralb@uludag.edu.tr
authors.forename: Haluk B
authors.initials: HB
authors.lastname: Oral
authors.forename: Ferah
authors.initials: F
authors.lastname: Budak
authors.forename: Esra Kunt
authors.initials: EK
authors.lastname: Uzaslan
authors.forename: Bilkay
authors.initials: B
authors.lastname: Baştürk
authors.forename: Ahmet
authors.initials: A
authors.lastname: Bekar
authors.forename: Halis
authors.initials: H
authors.lastname: Akalin
authors.forename: Ercüment
authors.initials: E
authors.lastname: Ege
authors.forename: Beyza
authors.initials: B
authors.lastname: Ener
authors.forename: Güher
authors.initials: G
authors.lastname: Göral
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics; D008297:Male; D020641:Polymorphism, Single Nucleotide; D016212:Transforming Growth Factor beta / Q000235:genetics; D014376:Tuberculosis / Q000235:genetics* / Q000276:immunology*; D014421:Turkey
chemical_list: D016207:Cytokines; C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D007371:Interferon-gamma