Difference in production of infectious wild-type measles and vaccine viruses in monocyte-derived dendritic cells.
Macrophages (Mø) and dendritic cells (DC) are thought to be targets of measles virus (MeV) at the early stage of infection. We compared the growth of Edmonston-derived vaccine strains and fresh clinical isolates of MeV in monocytes, monocyte-derived granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced Mø (GM-Mø) and in monocyte-derived DC (Mo-DC). Neither vaccine strains nor fresh isolates thrived in monocytes and GM-Mø and no differences were evident among them. On the other hand, infectious virus production was robust in Mo-DC infected with fresh isolates, but below the limits of detection in those infected with vaccine strains. Although the vaccine strains infected Mo-DC and replicated comparably with the fresh isolates, they accumulated far less matrix (M) protein. This was attributed to a difference in the stability of M protein produced in Mo-DC between the strains. Impaired production of infectious viruses in DC may be one cause of vaccine strain attenuation.
Ohgimoto
Kaori
K
Division of Microbiology, Kobe University Graduate School of Medicine, Kobe, Hyogo 650-0017, Japan.
Ohgimoto
Shinji
S
Ihara
Toshiaki
T
Mizuta
Hiroyuki
H
Ishido
Satoshi
S
Ayata
Minoru
M
Ogura
Hisashi
H
Hotta
Hak
H
D000818:Animals; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003713:Dendritic Cells / Q000378:metabolism / Q000821:virology*; D006801:Humans; D008457:Measles / Q000821:virology*; D009000:Monocytes / Q000378:metabolism / Q000821:virology*; D018103:Morbillivirus / Q000254:growth & development*; D014763:Viral Matrix Proteins / Q000378:metabolism
D014763:Viral Matrix Proteins