title: Novel streptomycin resistance gene from Mycobacterium fortuitum.
abstract: We have isolated the aph(3")-Ic gene, encoding an aminoglycoside 3"-O-phosphotransferase [APH(3")-Ic], from a genomic library of an environmental Mycobacterium fortuitum strain, selecting for streptomycin resistance. APH(3")-Ic phosphorylates and inactivates streptomycin. Similar genes have been described in Streptomyces griseus and plasmid RSF1010. It is also present in some M. fortuitum clinical isolates.
authors.affiliation: Departamento de Microbiología, Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad de Zaragoza, C/ Domingo Miral s/n, 50009-Zaragoza, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Ramón-García
authors.forename: Isabel
authors.initials: I
authors.lastname: Otal
authors.forename: Carlos
authors.initials: C
authors.lastname: Martín
authors.forename: Rafael
authors.initials: R
authors.lastname: Gómez-Lus
authors.forename: José A
authors.initials: JA
authors.lastname: Aínsa
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D029968:Escherichia coli Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000187:drug effects; D019907:Mycobacterium fortuitum / Q000187:drug effects* / Q000235:genetics*; D020102:Mycobacterium smegmatis / Q000187:drug effects; D010957:Plasmids / Q000235:genetics; D013307:Streptomycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; C104889:mraZ protein, E coli; D013307:Streptomycin