[Spectrophotometric determination of chondroitin sulfate with victory blue B].
In pH 4.0 acetic acid-sodium acetate buffer solution, cationic dye victory blue B shows an absorption peak at 614 nm, and the absorption peak decreases after it reacts with chondroitin sulfate to form association particles. The decrease in absorption value is linear with chondroitin sulfate concentration in the range of 0. 1-5 microg x mL(-1), and the correlation efficient is 0.9986. The method was applied to the determination of chondroitin sulfate in synthesis samples and real samples with rapidity, simplicity and good accuracy.
Chen
Yuan-yuan
YY
Department of Resource and Environmental Science, Guangxi Normal University, Guilin, China.
Jiang
Zhi-liang
ZL
Li
Zhen-zhong
ZZ
Zhou
Su-mei
SM
Sun
Shuang-jiao
SJ
D002809:Chondroitin Sulfates / Q000032:analysis*; D004396:Coloring Agents / Q000737:chemistry*; D012394:Rosaniline Dyes / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D004396:Coloring Agents; D012394:Rosaniline Dyes; D002809:Chondroitin Sulfates; C009662:Victoria blue B