title: Xenopus Tbx6 mediates posterior patterning via activation of Wnt and FGF signalling.
abstract: In vertebrates, the patterning of anterior-posterior (AP) axis is a fundamental process during embryogenesis. Wnt and FGF signalling pathways play important roles in regulating the patterning of embryo AP axis. Mouse Tbx6 encodes a transcription factor that has been demonstrated to be involved in the specification of the posterior tissue in mouse embryonic body. Here, we prove that morpholino-induced knockdown of XTbx6 impairs posterior development, indicating the requirement of XTbx6 in this process. Meanwhile, gain of XTbx6 function is sufficient to induce ectopic posterior structures in Xenopus embryos. Furthermore, XTbx6 activates the expression of Xwnt8 and FGF8, which are two mediators of posterior development, suggesting a mechanism by which XTbx6 modulates posterior patterning via Wnt and FGF signalling pathway activation.
authors.affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lou
authors.forename: Panfeng
authors.initials: P
authors.lastname: Fang
authors.forename: Shuangwei
authors.initials: S
authors.lastname: Li
authors.forename: Rui-Ying
authors.initials: RY
authors.lastname: Hu
authors.forename: Klaus-Michael
authors.initials: KM
authors.lastname: Kuerner
authors.forename: Herbert
authors.initials: H
authors.lastname: Steinbeisser
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ding
mesh_terms: D000818:Animals; D019521:Body Patterning; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D051379:Mice; D009024:Morphogenesis; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism*; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism
chemical_list: D016376:Oligonucleotides, Antisense; D020825:T-Box Domain Proteins; C491181:Tbx6 protein, Xenopus; D051153:Wnt Proteins; D029867:Xenopus Proteins; D005346:Fibroblast Growth Factors