title: Calprotectin and cross-linked N-terminal telopeptides in peri-implant and gingival crevicular fluid.
abstract: In a previous study on guided bone augmentation, a new collagen membrane was compared with an e-PTFE one on 28 partially edentulous patients who received 50 dental implants at stage 2 surgery. After implant integration and subsequent loading, we were able to recruit 22 patients with 22 implants and their contra-lateral corresponding teeth for longitudinal observation. Clinical parameters probing depth (PD), bleeding on probing (BoP), plaque index (PI), assessment of gingival crevicular fluid (GCF) and peri-implant crevicular fluid (PCF) volumes and periapical radiographs were performed at 2- and 3-year control appointments. Calprotectin (MRP 8/14) and cross-linked N-terminal telopeptide (NTx) levels in both crevicular fluids were determined by ELISA. PD was significantly reduced from years 2 to 3 appointments at implant sites as at teeth sites. At the 3-year appointment in both compartments, fluid volumes were significantly increased, which corresponded well with ascending NTx levels. The total amount of calprotectin decreased non-significantly in both GCF and PCF samples. Periapical radiographs revealed stable bone conditions around implants without significant changes from years 2 to 3 examinations. Clinical peri-implant parameters were considered as stable as the periodontal parameters of their corresponding teeth. A parallel increase in NTx levels in both GCF and PCF at 3-year appointment is not clearly understood; it may reflect an upregulation in the overall bone turnover rate.
authors.affiliation: Institute for Periodontology and Synoptic Dentistry, CharitéCenter 3, Universitätsmedizin Charité Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. anton.friedmann@charite.de
authors.forename: Anton
authors.initials: A
authors.lastname: Friedmann
authors.forename: Magdalena
authors.initials: M
authors.lastname: Friedrichs
authors.forename: Dogan
authors.initials: D
authors.lastname: Kaner
authors.forename: Bernd-Michael
authors.initials: BM
authors.lastname: Kleber
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Bernimoulin
mesh_terms: D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging; D000540:Alveolar Ridge Augmentation / Q000379:methods; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D024042:Collagen Type I / Q000032:analysis*; D015921:Dental Implants; D003774:Dental Plaque Index; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005884:Gingival Hemorrhage / Q000145:classification; D006801:Humans; D007576:Jaw, Edentulous, Partially / Q000000981:diagnostic imaging / Q000601:surgery; D039841:Leukocyte L1 Antigen Complex / Q000032:analysis*; D008137:Longitudinal Studies; D008567:Membranes, Artificial; D010455:Peptides / Q000032:analysis*; D010486:Periapical Tissue / Q000000981:diagnostic imaging; D010514:Periodontal Pocket / Q000145:classification; D011859:Radiography
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D015921:Dental Implants; D039841:Leukocyte L1 Antigen Complex; D008567:Membranes, Artificial; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide