New genus-specific primers for the PCR identification of novel isolates of the genus Streptomonospora.
The halophilic actinomycete genus Streptomonospora, forming a distinct branch in the 16S rRNA gene phylogenetic tree adjacent to the genera Nocardiopsis and Thermobifida, was first proposed by Cui et al. to accommodate the species type Streptomonospora salina. During a biodiversity and taxonomic study on halophilic filamentous actinomycetes from a saline lake in western China, numerous new halophilic actinomycetes strains were isolated. To confirm whether they are members of the genus Streptomonospora, one set of genus-specific oligonucleotides was designed which allows rapid detection of members of the genus Streptomonospora by means of PCR amplification. The genus specificity of these primers was validated with reference strains as well as with wild-type isolates, which exhibited morphological characteristics common to this genus.
Zhi
Xiao-Yang
XY
Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, People's Republic of China.
Tang
Shu-Kun
SK
Li
Wen-Jun
WJ
Xu
Li-Hua
LH
Jiang
Cheng-Lin
CL
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D002681:China; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D014871:Water Microbiology
D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S