Immobilization of UDP-galactose 4-epimerase from Escherichia coli on the yeast cell surface.
UDP-galactose 4-epimerase (EC 5.1.3.2, Gal E) from Escherichia coli catalyzes the reversible reaction between UDP-galactose and UDP-glucose. In this study, the Gal E gene from E. coli, coding UDP-galactose 4-epimerase, was cloned into pYD1 plasmid and then transformed into Saccharomyces cerevisiae EBY100 for expression of Gal E on the cell surface. Enzyme activity analyses with EBY100 cells showed that the enzyme displayed on the yeast cell surface was very active in the conversion between UDP-Glc and UDP-Gal. It took about 3 min to reach equilibrium from UDP-galactose to UDP-glucose.
Zhang
Hou-Cheng
HC
The State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, China. zhanghoucheng@sdu.edu.cn
Bi
Jin-Yan
JY
Chen
Chang
C
Huang
Gang-Liang
GL
Qi
Qing-Sheng
QS
Xiao
Min
M
Wang
Peng George
PG
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D019075:Electrophoresis, Capillary; D004800:Enzymes, Immobilized / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D014534:UDPglucose 4-Epimerase / Q000235:genetics / Q000378:metabolism*; D014531:Uridine Diphosphate Galactose / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D004269:DNA, Bacterial; D004800:Enzymes, Immobilized; D014531:Uridine Diphosphate Galactose; D014534:UDPglucose 4-Epimerase; D014532:Uridine Diphosphate Glucose