title: [A clinical study of intraocular malignant lymphoma].
abstract: OBJECTIVE
  We investigated whether vitreous cytology and the measurement of intravitreous cytokine were useful for the diagnosis of intraocular malignant lymphoma.
  
  
  METHODS
  8 eyes of 5 patients with suspected intraocular malignant lymphoma during the past 15 months. 3 vitreous samples were collected from 3 patients at the time of pars plana vitrectomy. Polymerase chain reaction(PCR) amplification and flowcytometric analysis(FACS) of the vitreous samples were performed. Interleukin (IL)-10 and IL-6 concentrations were measured.
  
  
  RESULTS
  Vitreous cytology showed increased atypical B lymphocytes. The vitreous IL-10/IL-6 ratio was higher than 1 in all cases. Monoclonal rearrangement of the immunoglobulin heavy chain gene and the light chain restriction of immunoglobulin were detected.
  
  
  CONCLUSIONS
  The detection of the monoclonality of infiltrated cells into the vitreous by PCR amplification and FACS, and the measurements of IL-10 and IL-6 concentrations in the vitreous fluid may be useful in the diagnosis of intraocular malignant lymphoma.
authors.affiliation: Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Ohta
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Ebihara
authors.forename: Yoshimune
authors.initials: Y
authors.lastname: Hiratsuka
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Akira
authors.initials: A
authors.lastname: Murakami
authors.forename: Kazuo
authors.initials: K
authors.lastname: Oshimi
mesh_terms: D000328:Adult; D000368:Aged; D005134:Eye Neoplasms / Q000175:diagnosis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D008223:Lymphoma / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014821:Vitrectomy; D014822:Vitreous Body / Q000473:pathology
chemical_list: D015850:Interleukin-6; D016753:Interleukin-10