title: Rapid detection of Arthroderma vanbreuseghemii in rabbit skin specimens by PCR-RFLP.
abstract: Three commercial rabbit farms independently reported suspected dermatophyte infections during 2004. Cultures confirmed that the infection was caused by the zoophilic Tricophyton mentagrophytes, while mating studies and sequencing of the internal transcribed spacer (ITS)1/4 polymerase chain reaction products, directly amplified from skin lesions and from pure culture isolates, established its Arthroderma vanbreuseghemii lineage. Subsequently, the corresponding DdeI restriction profiles of the ITS1/4 PCR products matched one of the two published T. mentagrophytes complex profiles. Sequence analysis of the published ITS sequences predicted distinct DdeI restriction sites for Arthroderma benhamiae and A. vanbreuseghemii, while all DdeI-generated restriction fragment length polymorphisms of the rabbit isolates were identical to those of A. vanbreuseghemii. The proposed procedure can be used for rapid dermatophyte detection and presumptive T. mentagrophytes identification.
authors.affiliation: Mycology Laboratory, Department of Microbiology, Medical School, University of Athens, Athens, Greece. marabatz@med.uoa.gr
authors.forename: Michael
authors.initials: M
authors.lastname: Arabatzis
authors.forename: Eftihia
authors.initials: E
authors.lastname: Xylouri
authors.forename: Irene
authors.initials: I
authors.lastname: Frangiadaki
authors.forename: Aleka
authors.initials: A
authors.lastname: Tzimogianni
authors.forename: Afroditi
authors.initials: A
authors.lastname: Milioni
authors.forename: George
authors.initials: G
authors.lastname: Arsenis
authors.forename: Aristea
authors.initials: A
authors.lastname: Velegraki
mesh_terms: D000818:Animals; D003883:Arthrodermataceae / Q000302:isolation & purification*; D003881:Dermatomycoses / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length; D011817:Rabbits / Q000382:microbiology*; D012867:Skin / Q000382:microbiology*