title: Hormonal change and cytokine mRNA expression in peripheral blood mononuclear cells during the development of canine autoimmune thyroiditis.
abstract: To elucidate the hormonal change and alteration in cytokine expression in peripheral blood mononuclear cells (PBMC) during the early stage of autoimmune thyroiditis, we have developed a canine model of this disease, in which normal dogs were immunized with bovine thyroglobulin (Tg) and/or canine thyroid extract. Serum samples were collected weekly, anti-canine Tg antibody was measured by enzyme-linked immunosorbent assay (ELISA) and thyroid stimulating hormone (TSH) and total T4 levels by radioimmunoassay. We also assayed T lymphocyte proliferation in response to Tg, as well as measuring cytokine mRNA by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). All six dogs immunized with bovine Tg had both canine Tg autoantibody and anti-T4 antibody. When the sample from the highest TgAA titre time-point was compared with baseline the expression of mRNA encoding the Th1-type cytokine such as interferon (IFN)-gamma, interleukin (IL)-18 and IL-15 was increased during the development of autoimmune thyroiditis. Expression of the Th2-type cytokine, IL-6 showed minimal change and IL-4 expression was not detected in any of the samples. Expression of the T suppressive cytokine, IL-10 and transforming growth factor (TGF)-beta was increased in the presence of antigen stimulation. These findings suggest that, although autoimmune thyroiditis is an organ-specific autoimmune disease, systemic cytokine mRNA expression is also changed.
authors.affiliation: Department of Veterinary Internal Medicine, Seoul National University, Seoul, Korea.
authors.forename: E-W
authors.initials: EW
authors.lastname: Choi
authors.forename: I-S
authors.initials: IS
authors.lastname: Shin
authors.forename: D-H
authors.initials: DH
authors.lastname: Bhang
authors.forename: D-H
authors.initials: DH
authors.lastname: Lee
authors.forename: B-K
authors.initials: BK
authors.lastname: Bae
authors.forename: M-S
authors.initials: MS
authors.lastname: Kang
authors.forename: D-Y
authors.initials: DY
authors.lastname: Kim
authors.forename: C-Y
authors.initials: CY
authors.lastname: Hwang
authors.forename: C-W
authors.initials: CW
authors.lastname: Lee
authors.forename: H-Y
authors.initials: HY
authors.lastname: Youn
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood; D002417:Cattle; D049109:Cell Proliferation; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D004195:Disease Models, Animal; D004285:Dogs; D007124:Immunoenzyme Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013954:Thyroglobulin / Q000276:immunology; D013961:Thyroid Gland / Q000276:immunology / Q000473:pathology; D013967:Thyroiditis, Autoimmune / Q000097:blood* / Q000276:immunology; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood* / Q000276:immunology; D014020:Tissue Extracts / Q000276:immunology
chemical_list: D001323:Autoantibodies; D016207:Cytokines; D012333:RNA, Messenger; D014020:Tissue Extracts; D013972:Thyrotropin; D013954:Thyroglobulin; D013974:Thyroxine