Localized induction of the ntcA regulatory gene in developing heterocysts of Anabaena sp. strain PCC 7120.
The ntcA gene encodes an N-control transcriptional regulator in cyanobacteria. In the N(2)-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, ntcA is an autoregulatory gene that is transcribed from a complex promoter region that includes a constitutive promoter (P(2)) and promoters that are induced upon N step-down (P(1) and P(3)). Expression of ntcA was investigated with the use of an ntcA-gfp translational fusion, which was introduced both in the natural ntcA locus and in a heterologous genomic place. Induction of ntcA-gfp took place after N step-down in all the cells of the filament, but at especially high levels in developing heterocysts. Localized induction could be driven independently by P(3) and P(1).
Olmedo-Verd
Elvira
E
Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Américo Vespucio 49, E-41092 Seville, Spain.
Muro-Pastor
Alicia M
AM
Flores
Enrique
E
Herrero
Antonia
A
D017033:Anabaena / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008856:Microscopy, Fluorescence; D009584:Nitrogen / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D024363:Transcription Initiation Site
D001426:Bacterial Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D009584:Nitrogen