A role for IL-1 receptor antagonist or other cytokines in the acute therapeutic effects of IVIg?
The exact mechanism of action of IVIg in the amelioration of immune thrombocytopenic purpura (ITP) is still unclear. Studies have suggested that IVIg may function through the regulation of cytokines, including interleukin-1 receptor antagonist (IL-1Ra), an inhibitor of phagocytosis. Using a mouse model relevant to ITP, we confirm an increase in mouse serum levels of IL-1Ra after exposure to IVIg, yet a recombinant IL-1Ra did not ameliorate thrombocytopenia. IVIg has also been shown to affect the expression of other regulatory cytokines. We have also recently established that IVIg specifically targets activating FcgammaRs on CD11c+ dendritic cells (DCs) as its primary mechanism of action in the amelioration of murine ITP. Herein, we show that IVIg functions therapeutically in mice lacking specific cytokines or their receptors that can potentially affect DC/macrophage function (IL-1 receptor, IL-4, IL-10, IL-12beta, TNF-alpha, IFN-gamma receptor, MIP-1alpha). This suggests that while IVIg may mediate the release of a variety of cytokines, the cytokines tested do not directly participate in the mechanism of IVIg action.
Crow
Andrew R
AR
Canadian Blood Services, Department of Laboratory Medicine, St Michael's Hospital, 30 Bond St, Toronto, ON, Canada M5B 1W8.
Song
Seng
S
Semple
John W
JW
Freedman
John
J
Lazarus
Alan H
AH
D000818:Animals; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines / Q000502:physiology*; D003713:Dendritic Cells / Q000276:immunology; D016756:Immunoglobulins, Intravenous / Q000494:pharmacology* / Q000627:therapeutic use; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics / Q000502:physiology*; D053631:Interleukin Receptor Common gamma Subunit / Q000172:deficiency / Q000502:physiology; D016753:Interleukin-10 / Q000172:deficiency / Q000502:physiology; D053762:Interleukin-12 Subunit p40 / Q000172:deficiency / Q000502:physiology; D015847:Interleukin-4 / Q000172:deficiency / Q000502:physiology; D019402:Macrophage Inflammatory Proteins / Q000172:deficiency / Q000502:physiology; D008264:Macrophages / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000502:physiology; D010976:Platelet Count; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology / Q000628:therapy*; D017452:Receptors, IgG / Q000187:drug effects; D017472:Receptors, Interleukin-1 / Q000172:deficiency / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000172:deficiency / Q000502:physiology
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines; C507764:Il1rn protein, mouse; D016756:Immunoglobulins, Intravenous; D053590:Interleukin 1 Receptor Antagonist Protein; D053631:Interleukin Receptor Common gamma Subunit; D053762:Interleukin-12 Subunit p40; D019402:Macrophage Inflammatory Proteins; D017452:Receptors, IgG; D017472:Receptors, Interleukin-1; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4; D009569:Nitric Oxide