Induction, purification and characterization of an antibacterial peptide scolopendrin I from the venom of centipede Scolopendra subspinipes mutilans.
The crude venom of the centipede Scolopendra subspinipes mutilans, injected with Escherichia coli K12D31 for 3-4 days showed broad-spectrum antimicrobial activity against Gram-positive. Gram-negative bacteria and fungi. It showed good antibacterial activity against E. coli K12D31 at different temperatures, pH, and ionic strengths. The crude venom was heated at 100 degrees C for 30 min, centrifuged at 10,000 rpm for 30 min at 4 degrees C and the supernatants were obtained, from which an antibacterial fraction having a molecular mass of 3000-5000 Da, was further separated by ultrafiltration. A homogeneous antibacterial peptide named scolopendrin I, having a molecular mass of 4,498 Da, was isolated using cation-exchange chromatography and two steps of reverse-phase high performance liquid chromatography (RP-HPLC). Scolopendrin I did not show any hemolytic and agglutination activities at the concentration below 30 microM.
Wenhua
Ren
R
Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210097, P.R. China.
Shuangquan
Zhang
Z
Daxiang
Song
S
Kaiya
Zhou
Z
Guang
Yang
Y
D000818:Animals; D000840:Anisoles / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification* / Q000502:physiology; D001180:Arthropod Venoms / Q000737:chemistry* / Q000378:metabolism; D001181:Arthropods / Q000737:chemistry* / Q000502:physiology; D007211:Indoles / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D000840:Anisoles; D023181:Antimicrobial Cationic Peptides; D001180:Arthropod Venoms; D007211:Indoles; C440201:scolopendrine