Determination of ruscogenin in crude Chinese medicines and biological samples by immunoassay.
Ruscogenin is a major bioactive steroidal aglycone found in the Chinese medicine, Ophiopogon japonicus. We have developed a quantitative determination of ruscogenin with an indirect enzyme-linked immunosorbent (ELISA) assay using polyclonal antibodies against ruscogenin conjugated with bovine serum albumin. This assay was highly sensitive, and it had considerably less cross-reactivity to diosgenin and sarsasapogenin, but high cross-reactivity to ruscogenin glycosides. The assay was successfully used for the measurement of ruscogenin concentrations in crude Chinese medicines and in biological samples from a pharmacokinetics study of ruscogenin.
Liu
Nan
N
Department of Complex Prescription of TCM, China Pharmaceutical University, 1 Shennong Road, Nanjing, 210038, People's Republic of China.
Wen
Xingbing
X
Liu
Jihua
J
Liang
Ming
M
Zeng
Huajin
H
Lin
Yining
Y
Yu
Boyang
B
D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D000941:Antigens / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D008297:Male; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D031441:Ophiopogon / Q000737:chemistry* / Q000276:immunology*; D010936:Plant Extracts / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D051381:Rats; D012680:Sensitivity and Specificity; D013150:Spirostans / Q000032:analysis* / Q000737:chemistry / Q000276:immunology* / Q000493:pharmacokinetics
D000906:Antibodies; D000941:Antigens; D010936:Plant Extracts; D013150:Spirostans; C012541:ruscogenin