title: Using triplet-triplet energy transfer to measure conformational dynamics in polypeptide chains.
abstract: Intrachain diffusion processes play an important role in protein folding and function. In this chapter we discuss the application of triplet-triplet energy transfer to directly measure rate constants for intrachain contact formation in polypeptide chains. The photochemistry of triplet-triplet energy transfer is described, experimental prerequisites of the method are discussed, and a detailed description of the experimental protocols and data analysis is given.
authors.affiliation: Department of Biophysical Chemistry, Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Beat
authors.initials: B
authors.lastname: Fierz
authors.forename: Karin
authors.initials: K
authors.lastname: Joder
authors.forename: Florian
authors.initials: F
authors.lastname: Krieger
authors.forename: Thomas
authors.initials: T
authors.lastname: Kiefhaber
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D010777:Photochemistry / Q000379:methods*; D011487:Protein Conformation; D017510:Protein Folding; D044004:Xanthones / Q000737:chemistry
chemical_list: D000596:Amino Acids; D010455:Peptides; D044004:Xanthones; C009689:xanthone