title: Real-time PCR TaqMan assay for detecting Trichophyton tonsurans, a causative agent of tinea capitis, from hairbrushes.
abstract: Tinea capitis caused by Trichophyton tonsurans is currently an epidemic in the United States, Europe, and Japan, and the cultivation of this microorganism is necessary for a definitive diagnosis. We recently developed a real-time PCR TaqMan assay as a culture-independent method for the rapid detection of T. tonsurans from hairbrushes.
authors.affiliation: Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Japan. sugita@my-pharm.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Sugita
authors.forename: Y
authors.initials: Y
authors.lastname: Shiraki
authors.forename: M
authors.initials: M
authors.lastname: Hiruma
mesh_terms: D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism; D014006:Tinea Capitis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D014249:Trichophyton / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D019914:Taq Polymerase