Invader plus method detects herpes simplex virus in cerebrospinal fluid and simultaneously differentiates types 1 and 2.
We report here on the development and validation of a prototype Invader Plus method for the qualitative detection of herpes simplex virus types 1 and 2 in cerebrospinal fluid (CSF). The method combines PCR and Invader techniques in a single, closed-tube, continuous-reaction format that gives an analytical sensitivity of approximately 10 copies per reaction. The clinical sensitivity and specificity were 100.0% and 98.6%, respectively, when the results of the method were validated against the results obtained with a PCR colorimetric microtiter plate system by use of clinical CSF specimens.
Allawi
Hatim T
HT
Third Wave Technologies, Inc., 502 South Rosa Rd., Madison, WI 53719, USA. hallawi@twt.com
Li
Haijing
H
Sander
Tamara
T
Aslanukov
Azamat
A
Lyamichev
Victor I
VI
Blackman
Amondrea
A
Elagin
Slava
S
Tang
Yi-Wei
YW
D020805:Central Nervous System Viral Diseases / Q000175:diagnosis / Q000821:virology; D002555:Cerebrospinal Fluid / Q000821:virology*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis / Q000134:cerebrospinal fluid; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods*; D006561:Herpes Simplex / Q000175:diagnosis / Q000821:virology; D018259:Herpesvirus 1, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013937:Thymidine Kinase / Q000235:genetics
D015342:DNA Probes; D004279:DNA, Viral; D013937:Thymidine Kinase