[Anti-oxidant effects of Tongxinluo on ATPase in focal brain ischemia-reperfusion rats].
OBJECTIVE
To explore the effects of Tongxinluo on adenosine triphosphatase (ATPase), anti-oxidant enzymes activities, and lipid peroxidation of mitochondria or brain homogenate in focal brain ischemia-reperfusion rats.


METHODS
The models of the focal brain ischemia-reperfusion rats were made by the middle cerebral artery occlusion (MCAO). Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and ATPase and malonaldehyde (MDA) levels of mitochondria or brain homogenate were measured by biochemical methods.


RESULTS
Tongxinluo significantly inhibited the decrease of activities of SOD and the increase of MDA levels, but had no difference in GSH-Px in brain homogenate. It also inhibited the decrease of activities of SOD, GSH-Px, ATPase, and the increase of MDA levels in mitochondria.


CONCLUSIONS
The protective mechanisms of Tongxinluo against mitochondrial injuries in focal ischemia-reperfusion rats may be derived from reducing lipid peroxides, scavenging free radicals and improving the energy metabolism.
Ding
Su-ju
SJ
Department of Neurology, Changhai Hospital of the Second Military Medical University, Shanghai 200433, China. dingsuju@hotmail.com
Li
Jia-si
JS
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002545:Brain Ischemia / Q000201:enzymology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000251:Adenosine Triphosphatases