Effect of sulforaphane on cell growth, G(0)/G(1) phase cell progression and apoptosis in human bladder cancer T24 cells.
Isothiocyanates (ITCs) from cruciferous vegetables have been shown to be effective in blocking initiation as well as progression of a range of chemically-induced tumors in animal models. In this study, sulforaphane, the most extensively studied ITC, was found to suppress the growth of T24 bladder cancer cells in vitro in a dose-dependent manner. Sulforaphane inhibited the proliferation of T24 cells with IC(5)0 values 26.9 and 15.9 microM following 24 and 48 h treatments. Sulforaphane (5-20 microM) induced early apoptosis and blocked cell cycle progression at G(0)/G(1) phase which was associated with upregulation of cyclin-dependent kinase inhibitor p27 expression. These results support a role for sulforaphane as an effective agent in the chemoprevention of bladder cancer.
Shan
Yujuan
Y
Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin 150081, P.R. China.
Sun
Changhao
C
Zhao
Xiujuan
X
Wu
Kun
K
Cassidy
Aedin
A
Bao
Yongping
Y
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D017879:Isothiocyanates; D013454:Sulfoxides; D013861:Thiocyanates / Q000494:pharmacology*; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000517:prevention & control
D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; D013454:Sulfoxides; D013861:Thiocyanates; D050760:Cyclin-Dependent Kinase Inhibitor p27; C016766:sulforaphane