title: Ganoderma lucidum extract stimulates glucose uptake in L6 rat skeletal muscle cells.
abstract: The effect of Ganoderma lucidum extract on glucose uptake was studied in L6 rat skeletal muscle cells. G. lucidum extract increased glucose uptake about 2-fold compared to control. The extract stimulated the activity of phosphatidylinositol (PI) 3-kinase which is a major regulatory molecule in the glucose uptake pathway. About 7-fold increased activity of a PI 3-kinase was observed after treatment with G. lucidum extract, whereas PI 3-kinase inhibitor, LY294002, blocked the G. lucidum extract-stimulated PI 3-kinase activity in L6 skeletal muscle cells. Protein kinase B, a downstream mediator of PI 3-kinase, was also activated by G. lucidum extract. We then assessed the activity of AMP-activated protein kinase (AMPK), another regulatory molecule in the glucose uptake pathway. G. lucidum extract increased the phosphorylation level of both AMPK alpha1 and alpha2. Activity of p38 MAPK, a downstream mediator of AMPK, was also increased by G. lucidum extract. Taken together, these results suggest that G. lucidum extract may stimulate glucose uptake, through both PI 3-kinase and AMPK in L6 skeletal muscle cells thereby contributing to glucose homeostasis.
authors.affiliation: Department of Pharmacology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Jung
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Ha
authors.forename: Mi-Ja
authors.initials: MJ
authors.lastname: Kim
authors.forename: Yoon Kyung
authors.initials: YK
authors.lastname: Uhm
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Hong
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Chung
authors.forename: Sung-Vin
authors.initials: SV
authors.lastname: Yim
mesh_terms: D055372:AMP-Activated Protein Kinases; D000818:Animals; D050260:Carbohydrate Metabolism / Q000187:drug effects; D018929:Cell Culture Techniques; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005947:Glucose / Q000493:pharmacokinetics*; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D020940:Reishi; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D055372:AMP-Activated Protein Kinases; D004365:Drugs, Chinese Herbal; D005947:Glucose; D020930:MAP Kinase Kinase Kinases; D009097:Multienzyme Complexes; D019869:Phosphatidylinositol 3-Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D051057:Proto-Oncogene Proteins c-akt; C069937:MAP3K8 protein, human