Active NDH-1 complexes from the cyanobacterium Synechocystis sp. strain PCC 6803.
We identified eight bands by staining native gels for NADPH-nitroblue tetrazolium oxidoreductase activity after electrophoresis of n-dodecyl-beta-d-maltoside-treated membranes of Synechocystis sp. strain PCC 6803. Among them, bands A, C, D and E were attributed to the activity of NADPH dehydrogenase (NDH-1). Band A is a highly active supercomplex of NDH-1 (about 1,000 kDa) that was absent in the DeltandhD1/D2 mutant and was suppressed under low CO(2). Band C was induced under low CO(2) or in the DeltandhD1/D2 mutant and was converted to bands D and E. Bands A and C appear to be an NDH-1L dimer and NDH-1M, respectively, with subunits essential for the activity.
Ma
Weimin
W
National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, PR China.
Deng
Young
Y
Ogawa
Teruo
T
Mi
Hualing
H
D002245:Carbon Dioxide / Q000378:metabolism; D019069:Cell Respiration / Q000235:genetics; D002478:Cells, Cultured; D019281:Dimerization; D004586:Electrophoresis; D004734:Energy Metabolism / Q000235:genetics; D005960:Glucosides; D009154:Mutation; D009252:NADPH Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D009580:Nitroblue Tetrazolium; D046939:Synechocystis / Q000201:enzymology* / Q000235:genetics; D020524:Thylakoids / Q000201:enzymology* / Q000235:genetics
D005960:Glucosides; D002245:Carbon Dioxide; D009580:Nitroblue Tetrazolium; C040358:dodecyl maltoside; D009252:NADPH Dehydrogenase