Activation of bombesin receptor subtype-3 stimulates adhesion of lung cancer cells.
Bombesin receptor subtype-3 (BRS-3) is an orphan G protein-coupled receptor having sequence homologies to gastrin-releasing peptide and neuromedin B receptors. [d-Phe6, beta-Ala11, Phe13, Nle14]bombesin(6-14) is known to act as a synthetic receptor agonist for BRS-3. To characterize BRS-3-mediated biological responses, we examined the effect of BRS-3 activation by [d-Phe6, beta-Ala11, Phe13, Nle14]Bn(6-14) on the adhesion of the small cell lung cancer NCI-N417 cells that express native BRS-3. We found that the BRS-3 agonist stimulated adhesion of NCI-N417 cells in laminin-coated culture wells. The adhesion of the cells to laminin induced by BRS-3 activation was accompanied by an increase in vinculin-like immunoreactivity and diminished in the presence of an anti-beta1 integrin antibody, suggesting that the receptor activation stimulates focal adhesion formation. We suggest that BRS-3 may be involved in invasion and metastasis of certain cancer cells, like small cell lung cancer cells, upon attachment to laminin.
Hou
Xinghua
X
Department of Molecular Physiology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, Japan. xhou@aecom.yu.edu
Wei
Li
L
Harada
Akihiro
A
Tatamoto
Kazuhiko
K
D001839:Bombesin / Q000031:analogs & derivatives / Q000494:pharmacology; D018288:Carcinoma, Small Cell / Q000737:chemistry / Q000378:metabolism*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D019012:Integrin beta1 / Q000032:analysis; D007797:Laminin / Q000378:metabolism; D008175:Lung Neoplasms / Q000737:chemistry / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D010456:Peptides, Cyclic; D018004:Receptors, Bombesin / Q000378:metabolism*; D013004:Somatostatin / Q000031:analogs & derivatives / Q000494:pharmacology; D016596:Vinculin / Q000032:analysis
C119436:(phenylalanyl(6)-alanyl(11)-phenylalanyl(13)-Nle(14))Bn(6-14); D019012:Integrin beta1; D007797:Laminin; D009842:Oligopeptides; D010446:Peptide Fragments; D010456:Peptides, Cyclic; D018004:Receptors, Bombesin; C107120:bombesin receptor subtype 3; C060347:lanreotide; D016596:Vinculin; D013004:Somatostatin; D001839:Bombesin