Intrinsic fluorescence polarization of amniotic fluid: II. Toward a noninvasive method for the determination of fetal lung maturity.
The present study compares two methods for the determination of fetal lung maturity: the novel intrinsic fluorescence polarization ratio (IFPR) and the commercial TDx-FLMII. Amniotic fluid (AF) samples were collected from 69 women during the second and third trimesters of singleton pregnancies. Thirty-three samples were tested for IFPR only after centrifugation, and the rest were examined both before and after centrifugation. Of the latter 33 samples, 29 were assessed for lung maturity with the TDx-FLMII method as well. The results showed that IFPR values decreased with the advance in gestational age (r = 0.77, p < 0.05, n = 69). A significant correlation was found between IFPR of centrifuged and noncentrifuged samples (r = 0.94, p < 0.05, n = 36). A significant correlation was demonstrated between IFPR and TDx-FLMII values of centrifuged (r = 0.75, p < 0.05, n = 29) and noncentrifuged (r = 0.63, p < 0.05, n = 29) samples and moreover, samples considered mature by TDx-FLMII had low values of IFPR (n = 10). It can be concluded that the IFPR method can utilize noncentrifuged AF, thus suggested as a potential noninvasive method.
Avraham
Hemy
H
Department of Electrical and Computer Engineering, Faculty of Engineering Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel.
Ohana
Eric
E
Maymon
Eli
E
Cohen-Luria
Rivka
R
Molcho
Jonathan
J
Parola
Abraham H
AH
D000653:Amniotic Fluid / Q000737:chemistry*; D005260:Female; D005454:Fluorescence Polarization; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third