Effects of the molecular weight of peg molecules (8, 20 and 35 KDA) on cell function and allograft survival prolongation in pancreatic islets transplantation.
The normopotassic solution SCOT (Macopharma, France) used for the isolation of the islets of Langerhans may improve both graft function and survival. We believe that this is due to the immunoprotective properties of polyethyleneglycol (PEG) (20 kDa; 1.5 mM/L), which is contained in this solution. However, the optimal PEG chain length remains to be determined. Three extracellular type solutions (SCOT without PEG) containing various PEG-8 kDa, 20 kDa, or 35 kDa- at 1.5 mM/L were compared in vitro for viscosity and osmolarity as well as in vivo using a murine model of pancreatic islet allotransplantation. We compared the effects of the various solutions on functional cell recovery (primary nonfunction rate, PNF) and immunoprotection (allograft survival time). We showed that the viscosity of PEG 35 kDa solutions was too high for physiological use. PEG 20 kDa solution provided the best graft function (0% PNF, P < .05). PEG 8 kda and 20 kDa solutions significantly increased allograft survival time compared to the PEG 35 kDa solution (P < .05). Graft survival was similar with PEG 20 kDa and PEG 8 kDa solutions: 27.50 +/- 3.70 days versus 23.13 +/- 4.39 days (NS). However, the number of PNF with PEG 8 kDa solution (50%) was significantly higher (P < .01) than that with the PEG 20 kDa solution (0%). These preliminary results indicated that the optimal chain length at 1.5 mM/L of PEG is 20 kDa.
Neuzillet
Y
Y
Faculte de Medecine Pitie-Salpetriere Universite Pierre et Marie Curie, Laboratoire d'Immunologie Cellulaire et Tissulaire, INSERM U543, 83 boulevard de l'hôpital, 75013 Paris, France.
Giraud
S
S
Lagorce
L
L
Eugene
M
M
Debre
P
P
Richard
F
F
Barrou
B
B
D000818:Animals; D006085:Graft Survival / Q000187:drug effects*; D016381:Islets of Langerhans Transplantation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008970:Molecular Weight; D019188:Organ Preservation Solutions / Q000494:pharmacology; D016035:Pancreas Transplantation / Q000379:methods* / Q000502:physiology; D011092:Polyethylene Glycols / Q000494:pharmacology*; D014184:Transplantation, Homologous; D014783:Viscosity
D019188:Organ Preservation Solutions; D011092:Polyethylene Glycols