[STR polymorphism of the non-coding human growth hormone gene (HUMGHCSA) and its use in personal identification--a preliminary report].
The HUMGHCSA locus, located within the non-coding region of the human growth hormone gene on chromosome 17, exhibiting polymorphism of the number of repeats of two and four nucleotide motifs (CT and CTTT), but also polymorphism caused by nucleotide substitution, is one of the most polymorphic STR markers. The purpose of this study was to report the development of a system of fluorescent analysis for an automatic sequencer, to test its reliability and to develop biostatistical parameters of its usefulness in forensic examinations. The studies, carried out on a group of 200 non-related individuals, showed the presence of 24 different alleles, of 221 to 279 bp in length. Biostatistical calculations demonstrated that the expected heterozygosity was 0,898634 +/- 0,015091, PD - 0,980586, PIC - 0,887709, PE - 0,792767, PE for motherless cases - 0,6556701, and the average paternity index - 4,932625. Such results indicate a High usefulness of the studied HUMGHCSA locus for identification examinations.
Spólnicka
Magdalena
M
Z Wydziału Biologii Centralnego Laboratorium Kryminalistycznego Komendy Głównej Policji.
Konarzewska
Magdalena
M
Sołtyszewski
Ireneusz
I
Berent
Jarosław
J
D016172:DNA Fingerprinting / Q000379:methods*; D018732:Forensic Anthropology / Q000379:methods*; D005820:Genetic Testing / Q000379:methods; D014644:Genetic Variation / Q000235:genetics; D005828:Genetics, Population; D005838:Genotype; D019382:Human Growth Hormone / Q000235:genetics*; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D011044:Poland; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D019382:Human Growth Hormone