title: A histochemical and immunohistochemical investigation of guanase and nedasin in rat and human tissues.
abstract: Human guanase is known as a specific enzyme in the liver, kidney, and brain. However, its functional significance remains poorly understood. In addition, interestingly, a different organ distribution between humans and rats was suggested. Here, we performed immunohistochemical staining with anti-human nedasin (neuronal and endocrine discs large/SAP102 associated protein), whose sequence was identical to that of guanase, antibody and histochemical staining for guanase in normal tissues of rat and human liver, kidney, and small intestine, and compared the results. Guanase activity was observed uniformly in the rat hepatocytes, biliary epithelium and vascular endothelium cells, while it was localized to the hepatocytes and biliary epithelium in the human liver. When the histochemical staining for guanase and the immunohistochemical staining for nedasin were compared, the stained regions were different in the rat liver but were almost consistent in all human tissues. Totally consistent staining results were also obtained between rats and humans in the other organization except the liver. Based upon the research reports to date, the experiments on guanase and nedasin in rat organs performed in this study are considered to have important implications in the investigation of their physiological significance.
authors.affiliation: Department of Digestive and Cardiovascular Medicine, The University of Tokushima Graduate School.
authors.forename: Katsutaka
authors.initials: K
authors.lastname: Sannomiya
authors.forename: Hirohito
authors.initials: H
authors.lastname: Honda
authors.forename: Kenichirou
authors.initials: K
authors.lastname: Kubo
authors.forename: Kunio
authors.initials: K
authors.lastname: Ii
authors.forename: Ying
authors.initials: Y
authors.lastname: Yuan
authors.forename: Eriko
authors.initials: E
authors.lastname: Aoyagi
authors.forename: Naoki
authors.initials: N
authors.lastname: Muguruma
authors.forename: Ichiro
authors.initials: I
authors.lastname: Shimizu
authors.forename: Susumu
authors.initials: S
authors.lastname: Ito
mesh_terms: D000619:Aminohydrolases / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D006148:Guanine Deaminase / Q000032:analysis / Q000378:metabolism*; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007421:Intestine, Small / Q000166:cytology / Q000201:enzymology*; D007668:Kidney / Q000166:cytology / Q000201:enzymology*; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000619:Aminohydrolases; C400750:nedasin; D006148:Guanine Deaminase