[Determination of rhodamine 123 in cell lysate by high-performance liquid chromatography with visible wavelength detection].
OBJECTIVE
To establish a high-performance liquid chromatography with visible wavelength detection method for rhodamine 123 in cell lysate.


METHODS
The HPLC separation was performed on a Kromasil C(18) (5 microm, 4.6 mm x 250 mm) column, using water (15 mmol/L potassium acetate and 1.2 mmol/L tetrabutylammonium bromide) - acetonitrile (65:35) as the mobile phase. The wave length was 390 nm, the internal standard was rhodamine B. Protein in the sample was precipitated by trichloroacetic acid.


RESULTS
The calibration curve was linear in the range of 3 - 300 microg/L. The intra-day and inter-day RSDs were less than 5%.


CONCLUSIONS
The method is accurate,sensitive, simple, and reliable for determining rhodamine 123 in cell lysate.
Song
Jian-ping
JP
Department of Pharmacy, Hunan Tumor Hospital, Changsha 410006, China. sjping333@126.com
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D020112:Rhodamine 123 / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods*
D020112:Rhodamine 123