title: Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction.
abstract: Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.
authors.affiliation: Department of Plant Biology, University of Georgia, Georgia 30602, USA.
authors.forename: Amy C
authors.initials: AC
authors.lastname: Luce
authors.forename: Anupma
authors.initials: A
authors.lastname: Sharma
authors.forename: Oliver S B
authors.initials: OS
authors.lastname: Mollere
authors.forename: Thomas K
authors.initials: TK
authors.lastname: Wolfgruber
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nagaki
authors.forename: Jiming
authors.initials: J
authors.lastname: Jiang
authors.forename: Gernot G
authors.initials: GG
authors.lastname: Presting
authors.forename: R Kelly
authors.initials: RK
authors.lastname: Dawe
mesh_terms: D002503:Centromere / Q000235:genetics*; D002843:Chromatin / Q000235:genetics*; D002874:Chromosome Mapping; D005819:Genetic Markers; D047468:Immunoprecipitation; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D003313:Zea mays / Q000235:genetics
chemical_list: D002843:Chromatin; D005819:Genetic Markers