Statistical properties of the band-broadening function.
Statistical properties of the band-broadening function calculated from the equilibrium and kinetic theories of Giddings and Eyring are compared with real liquid-chromatography elution curves of low-molecular-weight substances and with light-scattering elution curves of a high-molecular-weight polystyrene standard injected at several concentrations. The curves obtained by liquid chromatography agree in a large span of elution times with the theoretical ones and can be described by one value of the plate-height. The size exclusion chromatography (SEC) elution curves seem to approach the predicted statistical properties with decreasing concentration of injected polymer but, in general, a reliable prediction of band-broadening (BB) functions in SEC of polymers, especially of non-symmetric BB functions near the exclusion limit, seems not possible at this point. The relation of the theory with the characteristic-function approach developed by Dondi is also discussed.
Netopilík
Milos
M
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06 Prague 6, Czech Republic. netopil@imc.cas.cz
D002850:Chromatography, Gel / Q000706:statistics & numerical data; D002853:Chromatography, Liquid / Q000706:statistics & numerical data*; D015233:Models, Statistical; D012542:Scattering, Radiation