Cytoplasmic localization of the single glutamine synthetase in a unicellular red alga, Cyanidioschyzon merolae 10D.
Glutamine synthetase (GS) is a key enzyme for nitrogen assimilation. Although GS contains multiple molecular species found in plastid, mitochondria and cytoplasm in green plants and algae, genome analysis of a red alga, Cyanidioschyzon merolae, revealed a single nuclear gene for GS (CmGS). In this study, we experimentally determined the CmGS localization in the cytoplasmic compartment.
Terashita
Masaru
M
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan.
Maruyama
Shinichiro
S
Tanaka
Kan
K
D002458:Cell Fractionation; D003593:Cytoplasm / Q000201:enzymology; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D015151:Immunoblotting; D000461:Rhodophyta / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology
D005974:Glutamate-Ammonia Ligase