Magnetic targeting of bone marrow stromal cells into spinal cord: through cerebrospinal fluid.
We established a new magnetic targeting system in which bone marrow stromal cells migrate through the cerebrospinal fluid to the desired site in the spinal cord in rats. Subarachnoid injection has been reported as a minimally invasive method of transplantation of bone marrow stromal cells for spinal cord injury. It may be, however, less effective than direct injection into the spinal cord in terms of cell delivery. After implantation of a magnet, subarachnoid injection of bone marrow stromal cells labeled with magnetic beads was performed. Greater numbers of bone marrow stromal cells aggregated on the surface of the spinal cord owing to the magnetic force. This targeting system may be a useful tool in minimally invasive transplantation of bone marrow stromal cells for the treatment of spinal cord injury.
Nishida
Koji
K
Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. kl2nish@aol.com
Tanaka
Nobuhiro
N
Nakanishi
Kazuyoshi
K
Kamei
Naosuke
N
Hamasaki
Takahiko
T
Yanada
Shinobu
S
Mochizuki
Yu
Y
Ochi
Mitsuo
M
D000818:Animals; D030801:Animals, Genetically Modified; D016026:Bone Marrow Transplantation / Q000379:methods*; D002465:Cell Movement / Q000502:physiology; D002555:Cerebrospinal Fluid / Q000166:cytology* / Q000502:physiology; D003911:Dextrans; D004195:Disease Models, Animal; D052203:Ferrosoferric Oxide; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D007501:Iron; D007796:Laminectomy / Q000379:methods; D008280:Magnetics; D058185:Magnetite Nanoparticles; D008297:Male; D010087:Oxides; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000601:surgery*; D013129:Spinal Puncture / Q000379:methods; D017154:Stromal Cells / Q000502:physiology / Q000637:transplantation*
D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; D049452:Green Fluorescent Proteins; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide