Protein sulfation analysis--A primer.
The aim of this review is to present an overview of protein sulfation in the context of 'modificomics', i.e. post-translational modification-specific proteome research. In addition to a short introduction to the biology of protein sulfation (part 1), we will provide detailed discussion regarding (i) methods and tools for prediction of protein tyrosine sulfation sites (part 2), (ii) biochemical techniques used for protein sulfation analysis (part 3.1), and (iii) mass spectrometric strategies and methods applied to protein sulfation analysis (part 3.2). We will highlight strengths and limitations of different strategies and approaches (including references), providing a primer for newcomers to protein sulfation analysis.
Monigatti
Flavio
F
Department of Pathology/Enders 1130, Harvard Medical School and Children's Hospital Boston, 320 Longwood Ave, Boston, MA 02115, USA.
Hekking
Brian
B
Steen
Hanno
H
D000818:Animals; D004579:Electron Transport; D005169:Factor VIII / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008562:Membrane Glycoproteins / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000378:metabolism*; D019713:Receptors, CCR5 / Q000378:metabolism; D020539:Sequence Analysis, Protein / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015238:Sulfotransferases / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D014841:von Willebrand Factor / Q000378:metabolism
D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein; D011506:Proteins; D019713:Receptors, CCR5; D014841:von Willebrand Factor; C005662:tyrosine O-sulfate; D014443:Tyrosine; D005169:Factor VIII; D015238:Sulfotransferases; C039079:protein-tyrosine sulfotransferase