title: [Effect of anti-human DR5 monoclonal antibody on the apoptosis of human hepatocyte HL7702 cell lines].
abstract: OBJECTIVE
  To evaluate the effect of a novel anti-human DR5 monoclonal antibody (mAb mDRA-6) on the apoptosis of human hepatocyte HL7702 cell lines.
  
  
  METHODS
  DR5 expression on HL7702 cell surface was determined by flow cytometry(FCM). The effect of mAb mDRA-6 on the morphous of HL7702 cells was observed by fluorescence microscope. mDRA-6 cytotoxicity on HL7702 cells was detected by using MTT analysis. The rate of apoptosis was detected by FCM with Annexin V-FITC/PI staining.
  
  
  RESULTS
  HL7702 cells treated with mDRA-6 exhibited some typical apoptotic features in morphology. MTT analysis showed the death rate of HL7702 cells was 39% in the presence of 40 mg/L mDRA-6 for 6 hours. FCM detection indicated the apoptosis rate of the cells was 25.5% in the presence of 3 mg/L mDRA-6 for 6 hours with Annexin V-FITC/PI staining.
  
  
  CONCLUSIONS
  mDRA-6 can induce the apoptosis of HL7702 cells.
authors.affiliation: Key Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng 475003, China. zhangjun@henu.edu.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Yuan-fang
authors.initials: YF
authors.lastname: Ma
authors.forename: Guang-chao
authors.initials: GC
authors.lastname: Liu
authors.forename: Shu-lian
authors.initials: SL
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Lu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand