title: Antimicrobial activity of the methanolic extracts and compounds from Vismia laurentii De Wild (Guttiferae).
abstract: Methanolic extracts prepared from the leaves, twigs and the roots of Vismia laurentii De Wild as well as nine compounds isolated from these crude extracts, were tested for their antimicrobial activity against Gram-positive bacteria (six species), Gram-negative bacteria (12 species) and two Candida species using disc diffusion and well micro-dilution methods. The disc diffusion assay indicated that the crude extract was active against all the pathogens tested, whereas isolated compounds showed selective activities. The degree of sensitivity of the test organisms to purified compounds varied from 25 to 90%. Fridelin (8) was found to be the most active compound, while Bivismiaquinone (3) was the least active. The lowest minimum inhibition concentration (MIC) values as obtained by the micro-dilution assays were 19.53 and 1.22 microg/ml for the crude extracts and purified compounds, respectively. The lowest value for the purified compounds (1.22 microg/ml) was obtained with O(1)-demethyl-3',4'-deoxypsorospermin-3',4'-diol (6) on Candida gabrata and Bacillus subtilis; 1,8-dihydroxy-6-methoxy-3-methylanthraquinone (5) on Bacillus subtilis and 6-deoxyisojacareubin (7) on Bacillus stearothermophilus. These results provide promising baseline information for the potential use of these crude extracts as well as some of the isolated compounds in the treatment of bacterial and fungal infections.
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon. kuetevictor@yahoo.fr
authors.forename: Victor
authors.initials: V
authors.lastname: Kuete
authors.forename: Jean Robert
authors.initials: JR
authors.lastname: Nguemeving
authors.forename: Véronique Penlap
authors.initials: VP
authors.lastname: Beng
authors.forename: Anatole Guy Blaise
authors.initials: AG
authors.lastname: Azebaze
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Etoa
authors.forename: Michèle
authors.initials: M
authors.lastname: Meyer
authors.forename: Bernard
authors.initials: B
authors.lastname: Bodo
authors.forename: Augustin Ephrem
authors.initials: AE
authors.lastname: Nkengfack
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D028084:Clusiaceae / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D000432:Methanol / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts; D000432:Methanol