title: cDNA cloning of myosin heavy chain genes from medaka Oryzias latipes embryos and larvae and their expression patterns during development.
abstract: Several sarcomeric myosin heavy chains (MYHs) were cloned from embryos and larvae of medaka Oryzias latipes. Three genes encoding medaka MYHs (mMYHs) predominantly expressed in embryos (mMYH(emb1)) and larvae (mMYH(L1) and mMYH(L2)), all belonged to fast skeletal MYHs, showing spatiotemporally different expression patterns during development. Besides these mMYHs, a few novel mMYHs were cloned from embryos and larvae at hatching. Whereas mMYH(emb2), mMYH(emb3), and mMYH(L3) belonged to fast skeletal MYH, mMYH(C1) and mMYH(C2) did to slow/cardiac MYH. mMYH(emb1) was expressed ahead of mMYH(L1) and mMYH(L2). In situ hybridization analysis demonstrated that the transcripts of mMYH(emb1) and mMYH(C1) were located in the horizontal myoseptum, whereas those of mMYH(L1) and mMYH(L2) in the inner part of myotomes and pharyngeal muscles, and those of mMYH(C2) in the heart rudiment. In silico cloning based on the medaka genome database showed another mMYHs of the slow/cardiac types, mMYH(C3) and mMYH(C4).
authors.affiliation: Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Ono
authors.forename: Chunshi
authors.initials: C
authors.lastname: Liang
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ikeda
authors.forename: Shugo
authors.initials: S
authors.lastname: Watabe
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000737:chemistry / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics; D015870:Gene Expression; D016678:Genome / Q000235:genetics; D023281:Genomics; D017403:In Situ Hybridization; D007814:Larva / Q000235:genetics / Q000254:growth & development; D018995:Myosin Heavy Chains / Q000145:classification / Q000235:genetics*; D009990:Oryzias / Q000196:embryology* / Q000235:genetics / Q000254:growth & development*; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D018995:Myosin Heavy Chains