title: Proteomic study of plasma from moderate hypercholesterolemic patients.
abstract: Proteomics is a technology to detect and identify several proteins and their isoforms in a single sample. We used proteomics to analyze modifications in the protein map of plasma after simvastatin treatment of moderate hypercholesterolemic patients. Plasma from hypercholesterolemic patients (n = 9) was compared before and after 12 weeks of simvastatin treatment (40 mg/day). Patients with similar cardiovascular risk factors were used as controls (CR group). By using two-dimensional electrophoresis and mass spectrometry, we identified the different protein isoforms. The plasma expression of three fibrinogen gamma chain isoforms (FGG) was enhanced, whereas the expression of two isoforms of the fibrinogen beta chain (FGB) was reduced in the hypercholesterolemic patients compared with the CR group. The expression of apolipoprotein A-IV and three haptoglobin isoforms was higher in hypercholesterolemic patients. Simvastatin treatment modified the plasma expression of FGG chain isoform 1, FGB chain isoforms 1 and 2, vitamin D binding protein isoform 3, apo A-IV, and haptoglobin isoform 2. The modification of FGG chain isoform 1 and FGB chain isoforms 1 and 2 was positively correlated with total plasma cholesterol level. Proteomic analysis of plasma may help to know more in depth the molecular mechanism modified by simvastatin treatment.
authors.affiliation: Cardiovascular Research Unit, Cardiology Department, Cardiovascular Institute, Hospital Clínico San Carlos, Madrid 28040, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Alonso-Orgaz
authors.forename: Laura
authors.initials: L
authors.lastname: Moreno
authors.forename: Carlos
authors.initials: C
authors.lastname: Macaya
authors.forename: Luis
authors.initials: L
authors.lastname: Rico
authors.forename: Petra J
authors.initials: PJ
authors.lastname: Mateos-Cáceres
authors.forename: Daniel
authors.initials: D
authors.lastname: Sacristán
authors.forename: Francisco
authors.initials: F
authors.lastname: Pérez-Vizcaíno
authors.forename: Antonio
authors.initials: A
authors.lastname: Segura
authors.forename: Juan
authors.initials: J
authors.lastname: Tamargo
authors.forename: Antonio
authors.initials: A
authors.lastname: López-Farré
mesh_terms: D000595:Amino Acid Sequence; D001053:Apolipoproteins / Q000097:blood; D001798:Blood Proteins / Q000032:analysis* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005340:Fibrinogen / Q000032:analysis; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood* / Q000188:drug therapy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D040901:Proteomics / Q000379:methods*; D019821:Simvastatin / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D001053:Apolipoproteins; D001798:Blood Proteins; D005340:Fibrinogen; D019821:Simvastatin