title: Aporphine alkaloids and their reversal activity of multidrug resistance (MDR) from the stems and rhizomes of Sinomenium acutum.
abstract: Chromatographic separation of the MeOH extract from the stems and rhizomes of Sinomemium acutum led to the isolation of nine alkaloids and a lignan. Their structures were determined to be dauriporphine (1), bianfugecine (2), dauriporphinoline (3), menisporphine (4), (-)-syringaresinol (5), N-feruloyltyramine (6), acutumine (7), dauricumine (8), sinomenine (9), and magnoflorine (10) by spectroscopic means. These compounds were examined for their P-gp mediated MDR reversal activity in human cancer cells. Compound 1 showed the most potent P-gp MDR inhibition activity with an ED50 value 0.03 microg/mL and 0.00010 microg/mL in the MES-SA/DX5 and HCT15 cells, respectively.
authors.affiliation: Natural Products Laboratory, College of Pharmacy, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Yong Deuk
authors.initials: YD
authors.lastname: Min
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Choi
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
mesh_terms: D001060:Aporphines / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D006801:Humans; D018547:Plant Stems / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D032841:Sinomenium / Q000737:chemistry*
chemical_list: D001060:Aporphines