title: Decreased expression of annexin A1 is correlated with breast cancer development and progression as determined by a tissue microarray analysis.
abstract: Annexin A1 (ANXA1) is a calcium- and phospholipid-binding protein and a known mediator of glucocorticoid-regulated inflammatory responses. Using a combined multiple high-throughput approach, we recently identified a reduced expression of ANXA1 in human breast cancer. The finding was confirmed at the gene level by quantitative reverse transcription-polymerase chain reaction and at the protein level by immunohistochemical staining of normal, benign, and malignant breast tissues. In this study, we constructed and used a high-density human breast cancer tissue microarray to characterize the expressional pattern of ANXA1 according to histopathologies. The tissue microarray contains 1,158 informative breast tissue cores of different histologies including normal tissues, hyperplasia, in situ and invasive tumors, and lymph node metastases. Our results showed that there was a significant decrease in glandular expression of ANXA1 in ductal carcinoma in situ and invasive ductal carcinoma compared with either normal breast tissue or hyperplasia (P < .0001). Moreover, in benign breast tissue, myoepithelial cells showed strong expression of ANXA1. There was a decrease of ANXA1 expression in myoepithelial cells in ductal carcinoma in situ lesions compared with the same cell population in either normal or hyperplastic lesions. These results suggest that suppressed ANXA1 expression in breast tissue is correlated with breast cancer development and progression.
authors.affiliation: Gonda/UCLA Breast Cancer Research Laboratory, Revlon/UCLA Breast Center, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
authors.forename: Dejun
authors.initials: D
authors.lastname: Shen
authors.forename: Farzad
authors.initials: F
authors.lastname: Nooraie
authors.forename: Yahya
authors.initials: Y
authors.lastname: Elshimali
authors.forename: Victor
authors.initials: V
authors.lastname: Lonsberry
authors.forename: Jianbo
authors.initials: J
authors.lastname: He
authors.forename: Shikha
authors.initials: S
authors.lastname: Bose
authors.forename: David
authors.initials: D
authors.lastname: Chia
authors.forename: David
authors.initials: D
authors.lastname: Seligson
authors.forename: Helena R
authors.initials: HR
authors.lastname: Chang
authors.forename: Lee
authors.initials: L
authors.lastname: Goodglick
mesh_terms: D017305:Annexin A1 / Q000096:biosynthesis*; D001940:Breast / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000378:metabolism / Q000473:pathology; D018450:Disease Progression; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017305:Annexin A1