Interleukin-10 (IL-10) gene polymorphism as a potential host susceptibility factor in tuberculosis.
Several genes encoding for different cytokines may play crucial roles in host susceptibility to tuberculosis (TB), since the cytokine production capacity varies among individuals and depends on the cytokine gene polymorphism. The association of the cytokine gene polymorphisms with the development of TB was investigated in this study. DNA samples were obtained from a Turkish population of 81 patients with the different clinical forms of TB, and 50 healthy control subjects. All genotyping (IL-6, IL-10, IFN-gamma, TGF-beta and TNF-alpha) experiments were performed using sequence-specific primers PCR (PCR-SSP). Analysis of allele frequencies showed that IL-10 -1082 G allele frequency was significantly more common in TB patients than healthy controls (37.7% vs 23.0%, p: 0.014). No statistically significant differences were observed between the different clinical forms of the disease. These results suggest that the polymorphisms in IL-10 gene may affect susceptibility to TB and increase risk of developing the disease. To confirm the biological significance of our results, further studies should be performed on other population groups.
Oral
Haluk B
HB
Microbiology and Infectious Diseases Department, Faculty of Medicine, Uludağ University, 16059 Bursa, Turkey. oralb@uludag.edu.tr
Budak
Ferah
F
Uzaslan
Esra Kunt
EK
Baştürk
Bilkay
B
Bekar
Ahmet
A
Akalin
Halis
H
Ege
Ercüment
E
Ener
Beyza
B
Göral
Güher
G
D000483:Alleles; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics; D008297:Male; D020641:Polymorphism, Single Nucleotide; D016212:Transforming Growth Factor beta / Q000235:genetics; D014376:Tuberculosis / Q000235:genetics* / Q000276:immunology*; D014421:Turkey
D016207:Cytokines; C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D007371:Interferon-gamma