Ammonia removal from livestock wastewater by ammonia-assimilating microorganisms immobilized in polyvinyl alcohol.
We isolated ammonia-assimilating microorganisms from the livestock manure treatment systems and evaluated their ammonia-assimilating ability. Many isolates utilized ammonia at high rates when they were purely cultivated in a nitrogen-limited medium to which sterilized lagoon extract had been added. Some isolates that were immobilized in polyvinyl alcohol (PVA) utilized ammonia present in the media containing viable lagoon microorganisms. Staining with 4',6'-diamidino-2-phenylindole (DAPI) indicated that the immobilized high ammonia-assimilating isolates grew dominantly within the PVA beads. High ammonia-assimilating isolates in the mixed culture containing viable lagoon microorganisms were identified as Pseudomonas spp. and member of Rhizobiaceae species by partial sequencing of the 16S ribosomal DNA.
Sasaki
Hiraku
H
Laboratory of Animal Health and Management, Graduate School of Agricultural Science, Tohoku University, Naruko, Miyagi, 989-6711, Japan.
Nonaka
Jun
J
Sasaki
Takako
T
Nakai
Yutaka
Y
D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D000829:Animals, Domestic; D001709:Biotechnology / Q000379:methods; D018914:Cells, Immobilized; D003470:Culture Media; D007211:Indoles / Q000378:metabolism; D008372:Manure / Q000382:microbiology*; D008969:Molecular Sequence Data; D011142:Polyvinyl Alcohol; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012230:Rhizobiaceae / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D013194:Staining and Labeling; D014865:Waste Disposal, Fluid / Q000379:methods*
D003470:Culture Media; D007211:Indoles; D008372:Manure; D012336:RNA, Ribosomal, 16S; C007293:DAPI; D000641:Ammonia; D011142:Polyvinyl Alcohol