Speckle random coding for 2D super resolving fluorescent microscopic imaging.
In this manuscript we present a novel super resolving approach based upon projection of a random speckle pattern onto samples observed through a microscope. The projection of the speckle pattern is created by coherent illumination of the inspected pattern through a diffuser. Due to local interference of the coherent wave front with itself, a random speckle pattern is superimposed on the sample. This speckle pattern can be scanned over the object. A super-resolved image can be extracted from a temporal sequence of images by appropriate digital processing of the image stream. The resulting resolution is significantly higher than the diffraction limitation of the microscope objective. The new super-resolution method is demonstrated by application to fluorescence of biological samples.
Fixler
Dror
D
School of Engineering, Bar-Ilan University, Ramat-Gan 52900, Israel.
Garcia
Javier
J
Zalevsky
Zeev
Z
Weiss
Aryeh
A
Deutsch
Mordechai
M