title: Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases.
abstract: We cloned lipG, which encoded a lipolytic enzyme, from a Korean tidal flat metagenomic library. LipG was related to six putative lipases previously identified only in bacterial genome sequences. These enzymes comprise a new family. We partially characterized LipG, providing the first experimental data for a member of this family.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
authors.forename: Mi-Hwa
authors.initials: MH
authors.lastname: Lee
authors.forename: Choong-Hwan
authors.initials: CH
authors.lastname: Lee
authors.forename: Tae-Kwang
authors.initials: TK
authors.lastname: Oh
authors.forename: Jae Kwang
authors.initials: JK
authors.lastname: Song
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004952:Esters / Q000378:metabolism; D015698:Genomic Library; D019015:Geologic Sediments / Q000737:chemistry* / Q000382:microbiology; D007723:Korea; D008049:Lipase / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009574:Nitro Compounds / Q000378:metabolism; D010802:Phylogeny; D012623:Seawater / Q000737:chemistry* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D004952:Esters; D009574:Nitro Compounds; D008049:Lipase