Silica-guanidinium thiocyanate-based nucleic acid isolation protocol does not improve sensitivity of two commercial tests for detection of Mycobacterium tuberculosis in respiratory samples.
van Doorn
H R
HR
Department of Medical Microbiology, Room L1-245, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100DE, Amsterdam, The Netherlands. h.r.vandoorn@amc.uva.nl
Bruijnesteijn van Coppenraet
E S
ES
Duim
B
B
Vandenbroucke-Grauls
C M J E
CM
Weel
J F
JF
Dankert
J
J
Kuijper
E J
EJ
de Jong
M D
MD
D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D006146:Guanidines / Q000737:chemistry*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D009696:Nucleic Acids / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013183:Sputum / Q000382:microbiology; D013861:Thiocyanates / Q000737:chemistry*; D014376:Tuberculosis / Q000382:microbiology*
D006146:Guanidines; D009696:Nucleic Acids; D013861:Thiocyanates; C054436:guanidine thiocyanate; D012822:Silicon Dioxide