Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein.
The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.
Sarai
Naoyuki
N
RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan.
Kagawa
Wataru
W
Kinebuchi
Takashi
T
Kagawa
Ako
A
Tanaka
Kozo
K
Miyagawa
Kiyoshi
K
Ikawa
Shukuko
S
Shibata
Takehiko
T
Kurumizaka
Hitoshi
H
Yokoyama
Shigeyuki
S
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D004265:DNA Helicases / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D004277:DNA, Single-Stranded / Q000378:metabolism / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D051135:Rad51 Recombinase / Q000378:metabolism; D045522:Recombinases / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D011995:Recombination, Genetic
D018797:Cell Cycle Proteins; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C491221:RAD54B protein, human; D045522:Recombinases; D051135:Rad51 Recombinase; D000251:Adenosine Triphosphatases; C074556:DMC1 protein, human; D004265:DNA Helicases