Effects of tributyltin chloride on L-DOPA-induced cytotoxicity in PC12 cells.
Tributyltin chloride (TBTC) at concentrations of 0.5-1.0 microM inhibits dopamine biosynthesis in PC12 cells. In this study, the effects of TBTC on L-3,4-dihydroxyphenylalanine (L-DOPA)-induced cytotoxicity in PC12 cells were investigated. TBTC at concentrations up to 1.0 microM neither affected cell viability, nor induced apoptosis after 24 or 48 h in PC12 cells. However, TBTC at concentrations higher than 2.0 microM caused cytotoxicity through an apoptotic process. In addition, exposure of PC12 cells to non-cytotoxic (0.5 and 1.0 microM) or cytotoxic (2.0 microM) concentrations of TBTC in combination with L-DOPA (20, 50 and 100 microM) resulted in a significant increase in cell loss and the percentage of apoptotic cells after 24 or 48 h compared with TBTC or L-DOPA alone. The enhancing effects of TBTC on L-DOPA-induced cytotoxicity were concentration- and treatment time-dependent. These data demonstrate that TBTC enhances L-DOPA-induced cytotoxicity in PC 12 cells.
Lee
Jae Joon
JJ
College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University, Cheongju 361-763, Korea.
Kim
Yu Mi
YM
Park
Seung-Kook
SK
Lee
Myung Koo
MK
D000818:Animals; D000978:Antiparkinson Agents / Q000633:toxicity*; D017209:Apoptosis; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D007980:Levodopa / Q000633:toxicity*; D016716:PC12 Cells; D051381:Rats; D013997:Time Factors; D014220:Trialkyltin Compounds / Q000633:toxicity*
D000978:Antiparkinson Agents; D014220:Trialkyltin Compounds; D007980:Levodopa; C011559:tributyltin