Resonance scattering spectra of micrococcus lysodeikticus and its application to assay of lysozyme activity.
BACKGROUND
Several methods, including turbidimetric and colorimetric methods, have been reported for the detection of lysozyme activity. However, there is no report about the resonance scattering spectral (RSS) assay, which is based on the catalytic effect of lysozyme on the hydrolysis of micrococcus lysodeikticus (ML) and its resonance scattering effect.


METHODS



RESULTS
ML has 5 resonance scattering peaks at 360 400, 420, 470, and 520 nm with the strongest one at 470 nm. The concentration of ML in the range of 2.0x10(6)-9.3x10(8) cells/ml is proportional to the RS intensity at 470 nm (I(470 nm)). A new catalytic RSS method has been proposed for 0.24-40.0 U/ml (or 0.012-2.0 mug/ml) lysozyme activity, with a detection limit (3sigma) of 0.014 U/ml (or 0.0007 microg/ml). Saliva samples were assayed by this method, and it is in agreement with the results of turbidimetric method. The slope, intercept and the correlation coefficient of the regression analysis of the 2 assays were 0.9665, -87.50, and 0.9973, respectively.


CONCLUSIONS
The assay has high sensitivity and simplicity.
Jiang
Zhi-Liang
ZL
Department of Material Science and Chemical Engineering, Guilin University of Technology, Guilin 541004, China. zljiang@mailbox.gxnu.edu.cn
Huang
Guo-Xia
GX
D001433:Bacteriolysis / Q000187:drug effects; D001681:Biological Assay; D002478:Cells, Cultured; D006801:Humans; D008027:Light; D008837:Micrococcus / Q000166:cytology* / Q000187:drug effects; D009113:Muramidase / Q000032:analysis* / Q000494:pharmacology; D009391:Nephelometry and Turbidimetry; D012044:Regression Analysis; D012463:Saliva / Q000201:enzymology; D012542:Scattering, Radiation; D013057:Spectrum Analysis
D009113:Muramidase