C-reactive protein inhibits in vitro oxidation of low-density lipoprotein.
C-reactive protein (CRP) is elevated in cardiovascular disease and binds to oxidized phosphatidylcholine (oxPtC) in the low-density lipoprotein (LDL) surface. In the present study, we tested if CRP influences the susceptibility of LDL to oxidation. At physiological concentrations of 1-7mug/ml, CRP strongly inhibited copper-mediated oxidation of LDL and phospholipid liposomes in a concentration-dependent manner. Similar concentrations of different monoclonal antibodies or albumin did not influence LDL oxidation. Antioxidant activity of CRP was inhibited by phosphocholine (PC), indicating that the observed activity involves binding of CRP to oxPtC. These results suggest that CRP may limit atherogenic oxidation of LDL in vivo.
Rufail
Miguel L
ML
Department of Biochemistry, Virginia Commonwealth University, Medical College of Virginia Campus, Richmond, 23298, USA.
Ramage
Samuel C
SC
van Antwerpen
Rik
R
D000911:Antibodies, Monoclonal / Q000737:chemistry; D050197:Atherosclerosis / Q000378:metabolism; D002097:C-Reactive Protein / Q000737:chemistry* / Q000378:metabolism; D003300:Copper / Q000737:chemistry*; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry* / Q000378:metabolism; D008081:Liposomes; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism
D000911:Antibodies, Monoclonal; D008077:Lipoproteins, LDL; D008081:Liposomes; D010713:Phosphatidylcholines; D003300:Copper; D002097:C-Reactive Protein