Advances in the analysis of conformational transitions in peptides using differential scanning calorimetry.
Differential scanning calorimetry can measure the heat capacity of a protein/peptide solution over a range of temperatures at constant pressure, which is used to determine the enthalpy function of the system. There are several experimental factors that can have a significant impact on the determined enthalpy and subsequent derived thermodynamic parameters. These factors are discussed in terms of sample and instrument preparation, as well as data collection and analysis.
Streicher
Werner W
WW
Department of Biochemistry and Molecular Biology, Penn State College of Medicine, Hershey, PA, USA.
Makhatadze
George I
GI
D002152:Calorimetry, Differential Scanning / Q000379:methods*; D006358:Hot Temperature; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D013816:Thermodynamics
D010455:Peptides