ANKHD1, ankyrin repeat and KH domain containing 1, is overexpressed in acute leukemias and is associated with SHP2 in K562 cells.
In the present study, increased levels of ANKHD1 mRNA and protein expression in leukemia cell lines are reported, as compared with normal hematopoietic cells. Furthermore, a higher expression of ANKHD1 mRNA was detected in primary acute leukemia samples than in normal hematopoietic cells (P=0.002). ANKHD1 was detected in the cytosolic and membrane fraction of cells and was co-immunoprecipitated with SHP2 in protein extracts of K562 and LNCaP cell lines. These findings suggest a role for ANKHD1 as a scaffolding protein that may be associated with the abnormal phenotype of leukemia cells.
Traina
Fabíola
F
The Hematology and Hemotherapy Center, Faculty of Medical Sciences, Department of Internal Medicine, State University of Campinas, São Paulo, Brazil.
Favaro
Patricia M B
PM
Medina
Samuel de Souza
Sde S
Duarte
Adriana da Silva Santos
Ada S
Winnischofer
Sheila Maria Brochado
SM
Costa
Fernando F
FF
Saad
Sara T O
ST
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017089:Ankyrin Repeat; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D020014:K562 Cells; D007938:Leukemia / Q000378:metabolism*; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C489948:ANKK1 protein, human; D017346:Protein Serine-Threonine Kinases; C516724:PTPN11 protein, human; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D017027:Protein Tyrosine Phosphatases