[The expression of SDF-1/CXCR4 in trophoblasts and their role in maternofetal immune tolerance].
OBJECTIVE
To investigate the expression of SDF-1/CXCR4 in trophoblasts and their role in maternofetal immune tolerance.


METHODS
Extra-villous trophoblasts were highly purified from villi of first trimester. The expression of SDF-1 and CXCR4 in villi was detected by immunocytochemical staining. A chorioepithelioma cell line from trophoblasts with high expression of CXCR4 was screened by flow cytometry to test the chemotaxis of SDF-1 in transwell. The expression of SDF-1 and CXCR4 in villi of first trimester and placentas of last trimester was detected by immunohistochemical staining.


RESULTS
SDF-1 and CXCR4 were expressed in extra villous trophoblasts. In a certain coverage, the chemotactic activity of SDF-1 had positive correlation with its concentration (r=0.68, P<0.01), and the maximum CI (chemotactic index) was 1.62+/-0.12.


CONCLUSIONS
SDF-1 and CXCR4 play an important role in gestation and they are also important in maternofetal immune tolerance during pregnancy.
Yang
Yun
Y
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zou
Li
L
Wang
Yi-ying
YY
D000328:Adult; D045744:Cell Line, Tumor; D054377:Chemokine CXCL12 / Q000378:metabolism*; D002633:Chemotaxis; D005260:Female; D005786:Gene Expression Regulation; D054502:Histocompatibility, Maternal-Fetal; D006801:Humans; D007150:Immunohistochemistry; D011247:Pregnancy; D019718:Receptors, CXCR4 / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*
C515255:CXCL12 protein, human; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D019718:Receptors, CXCR4