title: Effects of ethylene and 1-methylcyclopropene (1-MCP) on gene expression and activity profile of alpha-1,4-glucan-phosphorylase during banana ripening.
abstract: Starch phosphorylases are enzymes that can use starch as substrate, and they are supposed to act in both in starch synthesis and degradation. This paper reports the effects of ethylene and 1-methylcyclopropene (1-MCP) on the degradation of starch and phosphorylase activity and gene expression. The results indicate that phosphorylase activity is induced during ripening and that it is associated with the onset of starch degradation. The regulation of banana phosphorylase activity is mainly dependent on gene expression, and the absence of ethylene perception by 1-MCP had a positive effect. However, this effect can be precluded by increased levels of ethylene, both autocatalytic and exogenous.
authors.affiliation: Faculdade de Ciências Farmacêuticas, Departamento de Alimentos e Nutrição Experimental, Universidade de São Paulo, 580 Butantã São Paulo, SP, Brazil CEP 05508-900.
authors.forename: Janaina Aparecida
authors.initials: JA
authors.lastname: Mainardi
authors.forename: Eduardo
authors.initials: E
authors.lastname: Purgatto
authors.forename: Adair
authors.initials: A
authors.lastname: Vieira
authors.forename: Walter Arato
authors.initials: WA
authors.lastname: Bastos
authors.forename: Beatriz Rosana
authors.initials: BR
authors.lastname: Cordenunsi
authors.forename: João Roberto
authors.initials: JR
authors.lastname: Oliveira do Nascimento
authors.forename: Franco Maria
authors.initials: FM
authors.lastname: Lajolo
mesh_terms: D003521:Cyclopropanes / Q000494:pharmacology*; D005030:Ethylenes / Q000494:pharmacology*; D005638:Fruit / Q000201:enzymology* / Q000254:growth & development; D015870:Gene Expression / Q000187:drug effects; D028521:Musa / Q000201:enzymology* / Q000254:growth & development; D006005:Phosphorylases / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D003521:Cyclopropanes; D005030:Ethylenes; D012333:RNA, Messenger; C036216:ethylene; D006005:Phosphorylases; C412563:1-methylcyclopropene