[Effect of ulinastatin on apoptosis in ileal mucosa of rats with hemorrhagic shock].
OBJECTIVE
To investigate the effect of ulinastatin on apoptosis in ileal mucosa of rats with hemorrhagic shock.


METHODS
A prospective, controlled animal study was performed. The rat model of hemorrhagic shock was replicated according to method described by Chaudry. After 60 minutes period of bleeding, rats were resuscitated by transfusion of shed blood and normal saline. A part of the animals were additionally treated with ulinastatin. The expression of tumor necrosis factor-alpha (TNF-alpha), malondialdehyde (MDA) content in serum, and expression of Bax, Bcl-2, caspase 3 protein in ileal mucosa were determined at different time points after reperfusion.


RESULTS
Compared with the normal saline group, the expression levels of TNF-alpha, MDA content in serum, Bax and caspase 3 protein in ileal mucosa during hemorrhagic shock after resuscitation were significantly increased, while Bcl-2 protein was markedly decreased. After fluid resuscitation, obvious increase in MDA, Bcl-2 protein, significant decrease in the level of TNF-alpha, the expression of Bax and caspase 3 protein in ileal mucosa were observed in the ulinastatin group compared with normal saline group.


CONCLUSIONS
Ulinastatin has protective effect on rats with hemorrhagic shock by suppressing the apoptosis in ileal mucosa.
Jiang
Long-yuan
LY
Department of Emergency, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, Guangdong, China. jly@china_em.com
Yang
Lian-hong
LH
Zhong
Wa
W
He
Zhi-jie
ZJ
Chang
Jian-xing
JX
Huang
Zi-tong
ZT
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006023:Glycoproteins / Q000494:pharmacology*; D007082:Ileum / Q000473:pathology*; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D011446:Prospective Studies; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D006023:Glycoproteins; D019253:Proto-Oncogene Proteins c-bcl-2; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D053148:Caspase 3; C028665:urinastatin