Identification of human semenogelin in membrane strip test as an alternative method for the detection of semen.
Semenogelin (Sg), a protein originating in the seminal vesicles and a substrate for prostate specific antigen (PSA or p30), is a useful marker for the identification of semen. And detection of Sg has been available commercially in a membrane test recently. PSA is commonly used to detect semen in forensic significant samples taken from sexual assault cases. The strip PSA test has been available commercially from various manufacturers for many years. In this study, we evaluated two immunochromatographic membrane tests, one for Sg and the other for PSA by analyzing human semen, other human bodily fluids/materials including urine, blood, saliva, sweat, breast milk, vaginal secretion and fecal materials, semen from various animals and forensic casework samples. The data demonstrate that both Sg and PSA strip tests provide rapid and sensitive method for identification of seminal plasma. These results show that the immunochromatographic method for Sg detection is useful for the identification of seminal plasma in forensic samples, an alternative to the method for PSA detection.
Pang
B C M
BC
Forensic Science Division, Hong Kong Government Laboratory, Homantin Government Offices, 88 Chung Hau Street, Kowloon, Hong Kong SAR, China. cmpang@govtlab.gov.hk
Cheung
B K K
BK
D000818:Animals; D015415:Biomarkers; D012634:Bodily Secretions / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry; D002415:Cats; D002417:Cattle; D004285:Dogs; D005243:Feces / Q000737:chemistry; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008297:Male; D017137:Nonoxynol; D017430:Prostate-Specific Antigen / Q000302:isolation & purification; D011902:Rape; D011934:Reagent Strips; D012661:Semen; D029607:Seminal Vesicle Secretory Proteins / Q000302:isolation & purification*; D013045:Species Specificity; D013089:Spermatocidal Agents; D013552:Swine
D015415:Biomarkers; D011934:Reagent Strips; D029607:Seminal Vesicle Secretory Proteins; D013089:Spermatocidal Agents; D017137:Nonoxynol; D017430:Prostate-Specific Antigen