Molecular cloning of streptomyces genes encoding vanillate demethylase.
The vanillate demethylase genes from Streptomyces sp. NL15-2K were cloned and sequenced. The vanA and vanB gene homologs, which encode the terminal oxygenase subunit (VanA) and the ferredoxin-type reductase subunit (VanB) of the enzyme respectively, were found in the sequenced 7.5-kb DNA region. Expression of the vanAB genes in Streptomyces lividans 1326 resulted in in vivo demethylation of veratric acid to vanillic acid.
Nishimura
Motohiro
M
Department of Chemical and Biological Engineering, Ube National College of Technology, Ube, Yamaguchi, Japan. nisimura@ube-k.ac.jp
Ishiyama
Daisuke
D
Davies
Julian
J
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010090:Oxidoreductases, O-Demethylating / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics*; D014641:Vanillic Acid / Q000031:analogs & derivatives / Q000378:metabolism
D004269:DNA, Bacterial; D011994:Recombinant Proteins; C009333:veratric acid; D010090:Oxidoreductases, O-Demethylating; C023284:vanillate O-demethylase; D014641:Vanillic Acid