[Increased sensitivity to chemotherapeutic drug by RNAi targeting Hsp701A in HepG2 cells].
OBJECTIVE
To investigate the effects on chemotherapeutic sensitivity to the drugs of tumor cells induced by Hsp701A targeting siRNA expression vector.


METHODS
Specific small interference RNA (siRNA) targeting Hsp701A gene was generated and its eukaryotic expression vector was constructed and transfected into human HepG2 cells.Sensitivity to the chemotherapeutic drug of HepG2 cells transfected with the vector was detected by MTT colorimetry.


RESULTS
The eukaryotic expression vector of siRNA targeting Hsp701A was successfully constructed and transfected into human HepG2 cells. Sensitivity to the chemotherapeutic drug of HepG2 cells was increased after Hsp701A was knock down (P<0.05).


CONCLUSIONS
Knockdown of Hsp701A gene by siRNA enhances sensitivity to chemotherapeutic drug in HepG2 cells.
Zhu
Qing
Q
Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
Zhang
Wang-gang
WG
Wang
Li-feng
LF
Wang
Fang
F
Zhang
Yong
Y
Yang
An-gang
AG
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004262:DNA Restriction Enzymes / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D050884:HSP72 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D000970:Antineoplastic Agents; D050884:HSP72 Heat-Shock Proteins; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes