Linear 3-hydroxybutyrate tetramer (HB4) produced by Sphingomonas sp. is characterized as a growth promoting factor for some rhizomicrofloral composers.
Sphingomonas spp. of alpha-proteobacteria often play a role in assisting the development of microfloral communities under adverse soil conditions. Using a Frateuria sp. as an indicator for bacterial growth assay, we investigated the bacterial growth-promoting factor in the culture fluids of Sphingomonas sp. EC-K085. This factor was successfully isolated and identified as linear (R,R,R,R)-3-hydroxybutyrate tetramer (HB4), having a hydroxy-end and a carboxy-end group. When 28 mug of HB4 was charged on a paper disc, impregnated Frateuria sp. cells in modified Winogradsky agar medium exhibited a promoted cell growth to form a clear colony emerging zone after a 2-day incubation.
Ogita
Noriko
N
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Hashidoko
Yasuyuki
Y
Limin
Suwido H
SH
Tahara
Satoshi
S
D020563:Gammaproteobacteria / Q000187:drug effects / Q000254:growth & development; D006885:Hydroxybutyrates / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009904:Optical Rotation; D011108:Polymers / Q000494:pharmacology; D012988:Soil Microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D020578:Sphingomonas / Q000737:chemistry / Q000378:metabolism
D006885:Hydroxybutyrates; D011108:Polymers