[Effects of Broad bean wilt virus 2 isolate infection on photosynthetic activities and chloroplast ultrastructure in broad bean leaves].
The effects of Broad bean wilt virus 2 (BBWV 2) isolate B935 and PV131 infection on photosynthetic activities and chloroplast ultrastructure in broad bean (Vicia faba) were investigated. As the disease progressed, net photosynthetic rate (P(n)), stomatal conductance (G(s)) of leaves, the chlorophyll content and chlorophyll a/b ratio decreased while the intercellular CO(2) concentration (C(i)) of leaves rose in comparison with that of the healthy plants (Figs. 1, 2). After BBWV 2 infection, F(v)/F(m), F(v)'/F(m)', Phi(PSII) and q(P) values all became lower, but the NPQ values were higher, than the healthy control plants (Fig. 3). Electron microscopy showed that BBWV 2 infection disrupted the chloroplast structure. Most of the B935-infected chloroplasts showed inhibition of lamellar development or membrane vesiculation (Fig. 4B, C) and PV131-infected chloroplasts showed swollen or disintegrated membrane (Fig. 4D-F). Chloroplasts of PV131-infected leaves were different from those of normal ones to a higher degree than those of the B935-infected ones, which suggests that the disruption of chloroplast structure by virus invasion is responsible for the suppression of photosynthesis, which is more serious with PV131 than with B935.
Li
Yan-Hong
YH
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China.
Hong
Jian
J
Xue
Li
L
Yang
Yong
Y
Zhou
Xue-Ping
XP
Jiang
De-An
DA
D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism / Q000648:ultrastructure*; D019181:Fabavirus / Q000502:physiology*; D054884:Host-Pathogen Interactions; D046529:Microscopy, Electron, Transmission; D010788:Photosynthesis / Q000502:physiology*; D018515:Plant Leaves / Q000502:physiology* / Q000648:ultrastructure / Q000821:virology; D031307:Vicia faba / Q000502:physiology / Q000648:ultrastructure / Q000821:virology*
D002734:Chlorophyll