title: Comparison of changes in circulating platelet-derived microparticles and platelet surface P-selectin expression after coronary stent implantation.
abstract: Platelet-derived microparticles (PDMPs) are released from activated platelets and may participate in the inflammatory process in response to vessel wall injury. This study was designed to compare the clinical significance of circulating PDMPs with that of P-selectin on the platelet membrane surface. In 20 patients with stable angina undergoing coronary stent implantation, circulating PDMPs were serially measured by enzyme-linked immunosorbent assay, and P-selectin expression on the surface of platelets was simultaneously analyzed by flow cytometry. PDMPs increased 24-48 h after coronary stenting in the coronary sinus (8.7 +/- 8.9 to 31.8 +/- 19.8 U/ml, P < 0.001) with a maximum at 48 h. In contrast, the mean channel fluorescence intensity for P-selectin increased 15 min after coronary stenting in the coronary sinus (19.5 +/- 5.6 to 25.2 +/- 7.5, P < 0.01) and remained elevated for 48 h; the changes were less striking in peripheral blood. The relative increase in PDMPs was not correlated with the increase in P-selectin expression at 15 min or 24 h after coronary stenting, but was correlated at 48 h (R = 0.48, P < 0.05). Both circulating PDMPs and P-selectin expression were enhanced in association with stent-induced platelet activation; however, the time course of changes in these two platelet activation markers was different. Therefore, the clinical relevance of circulating PDMPs may differ from that of P-selectin expression on the platelet membrane surface.
authors.affiliation: Faculty of Medicine, Department of Cardiovascular and Renal Medicine, Saga University, Saga, Japan. inouete@med.saga-u.ac.jp
authors.forename: Teruo
authors.initials: T
authors.lastname: Inoue
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Hikichi
authors.forename: Toshihumi
authors.initials: T
authors.lastname: Morooka
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Yoshida
authors.forename: Daisuke
authors.initials: D
authors.lastname: Fujimatsu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Komoda
authors.forename: Miho
authors.initials: M
authors.lastname: Kameda
authors.forename: Masako
authors.initials: M
authors.lastname: Nonaka
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Sohma
authors.forename: Shigemasa
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Koichi
authors.initials: K
authors.lastname: Node
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D000789:Angina, Unstable / Q000628:therapy; D001792:Blood Platelets / Q000378:metabolism*; D002462:Cell Membrane / Q000502:physiology*; D003331:Coronary Vessels / Q000502:physiology; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000378:metabolism*; D015539:Platelet Activation / Q000502:physiology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D015607:Stents; D013988:Ticlopidine / Q000494:pharmacology
chemical_list: D019007:P-Selectin; D010975:Platelet Aggregation Inhibitors; D013988:Ticlopidine