title: Prenatal-through-postnatal exposure to moderate levels of ethanol leads to damage on the hippocampal CA1 field of juvenile rats: a stereology and Golgi study.
abstract: Experimental paradigms conducted to assess the neurotoxic effects of ethanol exposure on hippocampus development have yielded controversial findings. Hippocampal CA1 population and some cytoarchitectural parameters of pyramidal cells were studied after exposure to ethanol during early development, in rats. Examination of 30-day-old offspring of rats exposed to moderate levels of ethanol during gestation through lactation showed an increased volume of the hippocampal CA1 field compared to untreated or pair-fed control pups, as well as a reduced number of pyramidal neurons. In addition, the number of spines from surviving CA1 pyramidal neurons was reduced. Furthermore, stubby and wide spines were proportionally increased, while the proportion of mushroom and ramified spines was reduced; no variation in the proportion of thin spines was observed. Because alcoholic women usually drink alcohol before, during, and after pregnancy, a broad-range experimental model of alcohol exposure was used in this study. The present findings show that experimental exposure to moderate levels of ethanol, resembling the human situation in alcoholic mothers, leads to loss of hippocampal CA1 pyramidal neurons, along with several pathological and plastic events in the dendritic arborization of these neurons. Some ethanol-induced excitotoxicity-related mechanisms, which may be underlying these effects, are discussed.
authors.affiliation: Laboratorio de Psicobiologia, Centro de Investigacion Biomedica de Michoacan, IMSS, Morelia, Mich, Mexico. igonbur@hotmail.com
authors.forename: I
authors.initials: I
authors.lastname: González-Burgos
authors.forename: M
authors.initials: M
authors.lastname: Alejandre-Gómez
authors.forename: M E
authors.initials: ME
authors.lastname: Olvera-Cortés
authors.forename: M I
authors.initials: MI
authors.lastname: Pérez-Vega
authors.forename: S
authors.initials: S
authors.lastname: Evans
authors.forename: A
authors.initials: A
authors.lastname: Feria-Velasco
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001835:Body Weight / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000633:toxicity*; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D006624:Hippocampus / Q000473:pathology*; D008098:Litter Size / Q000187:drug effects; D008297:Male; D009473:Neuronal Plasticity / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013534:Survival
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol