title: In vitro culture of tobacco callus on medium containing peptone and phytate leads to growth improvement and higher genetic stability.
abstract: Growth and genetic stability of Nicotiana tabacum L. callus were strongly improved by replacing the inorganic nitrogen and phosphorus of the Murashige and Skoog's medium by a soybean peptone and phytate, respectively. Cell proliferation after subcultivation on the modified medium was highly stimulated as evidenced by a strong biomass increase; this improvement was mainly due to the organic N source. In addition, while calluses grown under standard conditions displayed various cell sizes and DNA contents, subcultivation on the modified medium led to homogeneous cell size distribution and stable 4C-8C DNA contents through several subcultures. This improved genetic stability was due to replacement of inorganic P by phytate, provided the presence of peptone. Such new media composition could be useful for slow-growing cell suspensions or calluses.
authors.affiliation: Laboratoire de Cytologie expérimentale et Morphogenèse végétale, Université Pierre et Marie Curie-Paris 6, Site Ivry Le Raphaël, Case 150, 4 place Jussieu, 75252, Paris Cedex 05, France.
authors.forename: Guy
authors.initials: G
authors.lastname: Parc
authors.forename: Jacques
authors.initials: J
authors.lastname: Rembur
authors.forename: Philippe
authors.initials: P
authors.lastname: Rech
authors.forename: Dominique
authors.initials: D
authors.lastname: Chriqui
mesh_terms: D018533:Biomass; D049109:Cell Proliferation; D003470:Culture Media / Q000737:chemistry*; D018744:DNA, Plant; D010461:Peptones / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism*; D011003:Ploidies; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D018744:DNA, Plant; D010461:Peptones; D010833:Phytic Acid