A protein kinase signal-responsive gene carrier modified RGD peptide.
We have previously reported artificial gene-regulation systems responding to cyclic AMP-dependent protein kinase (PKA) using a cationic polymer. However, this polymer alone cannot deliver any gene into living cells. In the present work, we modified the signal-responsive polymer to the RGD peptide for the introduction of a polymer/DNA complex into living cells and succeeded in regulating the gene expression responding to intracellular PKA activation.
Oishi
Jun
J
Graduate School of Systems Life Sciences, Kyushu University, Motooka 744, Fukuoka 819-0395, Japan.
Ijuin
Moeko
M
Sonoda
Tatsuhiko
T
Kang
Jeong-Hun
JH
Kawamura
Kenji
K
Mori
Takeshi
T
Niidome
Takuro
T
Katayama
Yoshiki
Y
D045744:Cell Line, Tumor; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004789:Enzyme Activation; D005786:Gene Expression Regulation / Q000187:drug effects*; D015316:Genetic Therapy / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D016023:Integrins / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology*; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*
D016023:Integrins; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D004247:DNA; D008156:Luciferases; D011494:Protein Kinases