Degradation of fluorobenzene by Rhizobiales strain F11 via ortho cleavage of 4-fluorocatechol and catechol.
The aerobic metabolism of fluorobenzene by Rhizobiales sp. strain F11 was investigated. Liquid chromatography-mass spectrometry analysis showed that 4-fluorocatechol and catechol were formed as intermediates during fluorobenzene degradation by cell suspensions. Both these compounds, unlike 3-fluorocatechol, supported growth and oxygen uptake. Cells grown on fluorobenzene contained enzymes for the ortho pathway but not for meta ring cleavage of catechols. The results suggest that fluorobenzene is predominantly degraded via 4-fluorocatechol with subsequent ortho cleavage and also partially via catechol.
Carvalho
Maria F
MF
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal.
Ferreira
Maria Isabel M
MI
Moreira
Irina S
IS
Castro
Paula M L
PM
Janssen
Dick B
DB
D020561:Alphaproteobacteria / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002396:Catechols / Q000737:chemistry / Q000378:metabolism*; D003470:Culture Media; D005464:Fluorobenzenes / Q000378:metabolism*; D010101:Oxygen Consumption; D013379:Substrate Specificity
D002396:Catechols; D003470:Culture Media; D005464:Fluorobenzenes; C072305:4-fluorocatechol; C034221:catechol