title: Inhibition of poly(ADP-ribose)polymerase does not affect the recombination events in CHO xrs6 and wild type cells.
abstract: Activation of poly (ADP-ribose) polymerase -1 (PARP-1) is an early DNA damage response event that, together with phosphorylation of p53, prompts various cellular functions important in the maintenance of the genome stability. In mammalian cells, DSB are repaired by nonhomologous end-joining (NHEJ) and by homologous recombination (HR). To investigate the role of PARP-1 in HR, CHO-K1 wild type and xrs-6 mutant cell line were transfected with pLrec plasmids which carry two nonfunctional copies of the beta-galactosidase (lacZ) gene in a tandem array. In result of HR they can give rise to a functional copy of beta-galactosidase. To test whether PARP-1 affects the frequency of spontaneous and induced recombination repair, we treated CHO-K1 and xrs6 clones carrying chromosomally integrated pLrec with the PARP-1 inhibitor 3-aminobenzamide (3AB). Our results show that the spontaneous homologous intrachromosomal recombination frequency between the two lacZ copies was almost two orders of magnitude higher in xrs6 cells than in CHO-K1 cells, but that it was not affected by 3AB treatment. Induction of DNA damage by irradiation or electroporation of restriction enzymes did not significantly increase the recombination frequency. Furthermore, in both the cell lines, the effect of PARP-1 inhibition on DSB repair was examined using the neutral comet assay. There was no effect of 3AB treatment on DSB rejoining after 10 Gy irradiation. The results presented support the conclusion that PARP-1 is not directly involved in HR.
authors.affiliation: Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Dorodna 16, Warszawa, Poland. marylaw@ichtj.waw.pl
authors.forename: Maria
authors.initials: M
authors.lastname: Wojewódzka
authors.forename: Marcin
authors.initials: M
authors.lastname: Kruszewski
authors.forename: Tomasz
authors.initials: T
authors.lastname: Ołdak
authors.forename: Teresa
authors.initials: T
authors.lastname: Bartłomiejczyk
authors.forename: Aneta
authors.initials: A
authors.lastname: Goździk
authors.forename: Irena
authors.initials: I
authors.lastname: Szumiel
mesh_terms: D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D016466:CHO Cells; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects*; D017931:DNA Primers; D004260:DNA Repair / Q000502:physiology*; D018274:Electroporation; D004791:Enzyme Inhibitors; D005434:Flow Cytometry; D015162:Micronucleus Tests; D010957:Plasmids / Q000235:genetics; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011995:Recombination, Genetic / Q000502:physiology*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D001549:Benzamides; D017931:DNA Primers; D004791:Enzyme Inhibitors; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C025160:3-aminobenzamide; D001616:beta-Galactosidase