Dazl can bind to dynein motor complex and may play a role in transport of specific mRNAs.
Male germ cell development includes mitotic and meiotic cell divisions that are followed by dramatic morphological changes resulting in the production of spermatozoa. Genetic evidence has indicated that the DAZ family genes are critical for successful male germ cell development in diverse animals as well as humans. In the present study, we investigated the cellular functions of Dazl in the mouse male germ cells. We identified a specific interaction of Dazl with the dynein light chain, a component of the dynein-dynactin motor complex. The subcellular distribution of Dazl was microtubule-dependent and a selected number of Dazl-bound mRNAs could accumulate in the perinuclear area. Based on these results, we propose that Dazl may play a role in transport of specific mRNAs via dynein motor complex. The Dazl-bound mRNAs may be stored at specific sites and would be available for future developmental processes. Our study revealed the presence of an active mRNA transport system in mouse male germ cells.
Lee
Kyung Ho
KH
Department of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University, Seoul, Korea.
Lee
Seongju
S
Kim
Byunghyuk
B
Chang
Sunghoe
S
Kim
Soo Woong
SW
Paick
Jae-Seung
JS
Rhee
Kunsoo
K
D000818:Animals; D001693:Biological Transport, Active; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D000072159:Dynactin Complex; D004398:Dyneins / Q000378:metabolism*; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D008954:Models, Biological; D020409:Molecular Motor Proteins / Q000378:metabolism; D046912:Multiprotein Complexes; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
C508515:DAZL protein, mouse; C430864:DLC-1 (deleted in liver cancer) protein, mouse; D000072159:Dynactin Complex; D020690:GTPase-Activating Proteins; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D046912:Multiprotein Complexes; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D025521:Tumor Suppressor Proteins; D004398:Dyneins