Precipitation of Zn(II), Cu(II) and Pb(II) at bench-scale using biogenic hydrogen sulfide from the utilization of volatile fatty acids.
Biological production of hydrogen sulfide (H(2)S) using sulfate-reducing bacteria (SRB) has important potential within environmental biotechnology. The aim of this work was to study the possibility of using SRB for the treatment of an acid mine drainage (AMD) at bench-scale. This process involved three stages: the optimization of H(2)S production through the utilization of total volatile fatty acids (TVFAs) by SRB, the establishment of a biofilm reactor for sulfide production, and the precipitation of metals by using the biologically produced H(2)S. The substrates used for TVFAs production consisted of papaya, apple and banana. The H(2)S produced from the degradation of TVFAs was utilized for the precipitation of a metal-contaminated effluent collected from Bolivar mine (Oruro, Bolivia). The maximum concentration of H(2)S obtained was approximately 16mM. Removal efficiencies of ca. 100% for copper, above 94% for zinc, and above 92% for lead were achieved.
Alvarez
Maria Teresa
MT
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Getingevagen 60, P.O. Box 124, S-22100 Lund, Sweden.
Crespo
Carla
C
Mattiasson
Bo
B
D001419:Bacteria / Q000378:metabolism*; D018441:Biofilms; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D001838:Bolivia; D005227:Fatty Acids / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry; D006862:Hydrogen Sulfide / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D019216:Metals, Heavy / Q000378:metabolism*; D008906:Mining; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D014876:Water Pollution / Q000517:prevention & control*
D005227:Fatty Acids; D019216:Metals, Heavy; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D006862:Hydrogen Sulfide