In vitro antioxidant studies on the benzyl tetra isoquinoline alkaloid berberine.
Berberine is a benzyl tetra isoquinoline alkaloid which is widely used as an antimicrobial and an antidiarrhoeal. As berberine containing plants are virtually used in all forms of traditional medicine, our study aimed to examine the antioxidant activity of berberine using 2,2-diphenyl 1-picrylhydrazyl (DPPH) radical scavenging, nitric oxide scavenging, lipid peroxidation, superoxide scavenging, iron chelating activity and 2,2-azino bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical scavenging methods. The IC(50) values for all the models were calculated by regression analysis. In all the models tested, berberine showed its ability to scavenge the free radicals in a concentration dependent manner. The present study thereby justifies the therapeutic potential of berberine.
Shirwaikar
Annie
A
Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal, Karnataka, India. annieshirwaikar@yahoo.com
Shirwaikar
Arun
A
Rajendran
Kuppusamy
K
Punitha
Isaac Sam Raj
IS
D000975:Antioxidants / Q000494:pharmacology*; D052160:Benzothiazoles; D001599:Berberine / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide; D016166:Free Radical Scavengers / Q000494:pharmacology; D007501:Iron / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D013451:Sulfonic Acids; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D052160:Benzothiazoles; D016166:Free Radical Scavengers; D013451:Sulfonic Acids; D001599:Berberine; D013481:Superoxides; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D009569:Nitric Oxide; D007501:Iron; D004121:Dimethyl Sulfoxide