title: Inhibitory effects of tilianin on the expression of inducible nitric oxide synthase in low density lipoprotein receptor deficiency mice.
abstract: We investigated the effect of tilianin upon inducible nitric oxide synthesis in the plasma of low-density lipoprotein receptor knock-out (Ldlr-/-) mice fed with high cholesterol diet and in primary peritoneal macrophages of Ldlr-/- mice. High cholesterol diet induced nitric oxide production in the plasma of Ldlr-/- mice. Tilianin reduced the level of nitric oxide (NO) in plasma from Ldlr-/- mice induced by the high cholesterol diet. Tilianin also inhibited the NO production from the primary culture of peritoneal macrophages treated with lipopolysaccharide. The inhibition of NO production was caused by the suppression of inducible nitric oxide synthase (iNOS) gene expression in peritoneal macrophages isolated from Ldlr-/- mice. Moreover, tilianin inhibited the transcriptional activation of iNOS promoter that has NF-kappaB binding element. Thus, these results provide the first evidence that tilianin inhibit iNOS expression and production of NO and may act as a potential anti-inflammatory agent.
authors.affiliation: Division of Molecular Life Sciences and Center for Cell Signaling Research, Ewha Womans University, Seoul 120-750, Korea.
authors.forename: Ki-Hoan
authors.initials: KH
authors.lastname: Nam
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Seo
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Yong-Sung
authors.initials: YS
authors.lastname: Won
authors.forename: Mi-Ran
authors.initials: MR
authors.lastname: Lee
authors.forename: Mi-Ni
authors.initials: MN
authors.lastname: Lee
authors.forename: Jong-Gil
authors.initials: JG
authors.lastname: Park
authors.forename: Young-Myeong
authors.initials: YM
authors.lastname: Kim
authors.forename: Hyoung-Chin
authors.initials: HC
authors.lastname: Kim
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Lee
authors.forename: Hyeong-Kyu
authors.initials: HK
authors.lastname: Lee
authors.forename: Sei-Ryang
authors.initials: SR
authors.lastname: Oh
authors.forename: Goo Taeg
authors.initials: GT
authors.lastname: Oh
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology*; D007249:Inflammation / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis / Q000097:blood; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011973:Receptors, LDL / Q000235:genetics*; D012850:Sinus of Valsalva / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D014018:Tissue Distribution; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D005419:Flavonoids; D006027:Glycosides; D016328:NF-kappa B; D011973:Receptors, LDL; C426884:tilianin; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; D052247:Nitric Oxide Synthase Type II