Real-time PCR TaqMan assay for detecting Trichophyton tonsurans, a causative agent of tinea capitis, from hairbrushes.
Tinea capitis caused by Trichophyton tonsurans is currently an epidemic in the United States, Europe, and Japan, and the cultivation of this microorganism is necessary for a definitive diagnosis. We recently developed a real-time PCR TaqMan assay as a culture-independent method for the rapid detection of T. tonsurans from hairbrushes.
Sugita
T
T
Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Japan. sugita@my-pharm.ac.jp
Shiraki
Y
Y
Hiruma
M
M
D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism; D014006:Tinea Capitis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D014249:Trichophyton / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*
D019914:Taq Polymerase