Proliferative arrest and activation of apoptosis related genes in Rolly Protein-silenced cells.
Here we describe a novel small polypeptide expressed in chick embryo and mouse adult tissues referred to as Rolly Protein (Rolp), expressed at the highest levels in tibial cartilage and lung respectively. Investigating its putative role in cartilage differentiation we found that its expression is restricted to proliferative stages consistently with a decreased proliferation rate observed in Rolp-silenced cells. Additional functional studies demonstrate that inhibition of Rolp expression causes a transcription modulation of genes involved in apoptosis. The results here provided strongly suggest an active role of Rolp in the control of cell proliferation and apoptosis.
Pagano
Aldo
A
Dipartimento di Oncologia Biologia e Genetica, Università di Genova, Italy. aldo.pagano@istge.it
Tonachini
Laura
L
Monticone
Massimiliano
M
Tortelli
Federico
F
Castelnuovo
Manuele
M
Randazzo
Nadia
N
Tavella
Sara
S
Di Marco
Eddi
E
Cancedda
Ranieri
R
Castagnola
Patrizio
P
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D049109:Cell Proliferation; D002478:Cells, Cultured; D002642:Chick Embryo; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D020219:Chondrogenesis / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D020868:Gene Silencing; D016350:Leucine Zippers / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D051017:Apoptosis Regulatory Proteins; D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; C000589631:Rolly protein, mouse