title: Development of gas chromatography-mass spectrometry following microwave distillation and simultaneous headspace single-drop microextraction for fast determination of volatile fraction in Chinese herb.
abstract: In this work, for the first time, microwave distillation (MD) coupled with simultaneous headspace single-drop microextraction (HS-SDME) was developed for the determination of the volatile components in the Chinese herb, Artemisia capillaris Thunb. The volatile components were rapidly isolated by MD, and simultaneously extracted and concentrated by using a dodecane microdrop. The volatile oil extracted in the microdrop solvent was analyzed by gas chromatography-mass spectrometry (GC-MS). The experimental parameters of solvent selection, microdrop volume, microwave power, irradiation time and sample amount were investigated, and the method precision was also studied. The optimal parameters were extraction solvent of dodecane, solvent volume of 2.0 microL, microwave power of 400 W, irradiation time of 4 min, and sample amount of 2.0 g. Thirty-five volatile compounds present in Artemisia capillaris Thunb. were identified by using the proposed method, which were identical with those obtained by the conventional steam distillation method. The experimental results showed that MD-HS-SDME is a simple, rapid, reliable, and solvent-free technique for the determination of volatile compounds in Chinese herbs.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai 200433, China. chdeng@fudan.edu.cn
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Yu
authors.initials: Y
authors.lastname: Mao
authors.forename: Fengli
authors.initials: F
authors.lastname: Hu
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D019306:Artemisia / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008872:Microwaves; D009822:Oils, Volatile / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile