title: A combined shotgun and multidimensional proteomic analysis of the insoluble subproteome of the obligate thermophile, Geobacillus thermoleovorans T80.
abstract: To further our understanding of the biology of the thermophilic bacterium Geobacillus thermoleovorans T80, we now report the first proteomic analysis of the insoluble subproteome of this isolate. A combination of both shotgun and multidimensional methodologies were utilized, and a total of 8628 peptides was initially identified by automated MS/MS identification software. Curation of these peptides led to a final list of 184 positive protein identifications. The proteins from this insoluble subproteome were functionally classified, and physiochemical characterization was carried out. Of 15 hypothetical conserved proteins identified, we have assigned function to all but four. A total of 31 proteins were predicted to possess signal peptides. In silico investigation of these proteins allowed us to identify four of the five bacterial classes of signal peptide, namely, (i) twin-arginine translocation; (ii) Sec-type; (iii) lipoprotein, and (iv) ABC transport. In addition, a number of proteins were identified that are known to be involved in the transport of compatible solutes, known to be important in microbial stress responses.
authors.affiliation: School of Biomedical Sciences, University of Ulster, Coleraine, County Londonderry BT52 1SA, United Kingdom. rl.graham@ulster.ac.uk
authors.forename: Robert Leslie James
authors.initials: RL
authors.lastname: Graham
authors.forename: S Naomi
authors.initials: SN
authors.lastname: O'Loughlin
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Pollock
authors.forename: Nigel G
authors.initials: NG
authors.lastname: Ternan
authors.forename: D Brent
authors.initials: DB
authors.lastname: Weatherly
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Jackson
authors.forename: Rick L
authors.initials: RL
authors.lastname: Tarleton
authors.forename: Geoff
authors.initials: G
authors.lastname: McMullan
mesh_terms: D000595:Amino Acid Sequence; D001406:Bacillaceae / Q000737:chemistry*; D001426:Bacterial Proteins / Q000032:analysis* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002853:Chromatography, Liquid; D019295:Computational Biology / Q000379:methods; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D040901:Proteomics / Q000379:methods*
chemical_list: D001426:Bacterial Proteins