title: Numerical model for DNA loading in microdevices: stacking and autogating effects.
abstract: Many electrophoresis-based DNA sequencing and genotyping microdevices rely on field-driven effects to load and preconcentrate the sample. A quantitative model is developed for a broad class of electrophoresis-based microfabricated sample injectors. Quantitative predictions of DNA preconcentration are compared with experimental data and are shown to qualitatively reproduce the detailed time-evolving sample distribution in the injector. The model provides practical guidance on device and protocol design, in order to optimize this critical aspect of microfluidic devices.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
authors.forename: Sameh A
authors.initials: SA
authors.lastname: El-Difrawy
authors.forename: Alok
authors.initials: A
authors.lastname: Srivastava
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Gismondi
authors.forename: Brian K
authors.initials: BK
authors.lastname: McKenna
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Ehrlich
mesh_terms: D004247:DNA / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000592:standards*; D046209:Electrophoresis, Microchip / Q000592:standards*; D017022:Flow Injection Analysis; D008956:Models, Chemical; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA