In vivo antidiabetic and antioxidant potential of Helichrysum plicatum ssp. plicatum capitulums in streptozotocin-induced-diabetic rats.
Helichrysum species (Asteraceae) are widely found in Anatolia. Decoction prepared from the capitulums of Helichrysum plicatum ssp. plicatum is used to alleviate the symptoms of diabetes mellitus in folk medicine. In the present study, the hypoglycaemic and antioxidant potential of Helichrysum plicatum ssp. plicatum was evaluated by using in vivo methods in normal and streptozotocin-induced-diabetic rats. After the oral administration of water and ethanolic extracts at doses of 500mg/kg body weight prepared from the capitulums of plant, blood glucose levels were monitored at specific intervals. Tolbutamide was used as a reference drug at a dose of 100mg/kg. The experimental data indicated that water and ethanol extracts of capitulums demonstrate significant antihyperglycaemic and antioxidant activity in streptozotocin-induced rats which confirmed the folkloric utilization. In order to assess the role of polyphenolic components in the relevant activity, phenolic and flavonoid contents of each extract were also determined in terms of total phenols: 113.5+/-8.6mg (gallic acid equivalent/1g extract) and total flavanoids 50.5+/-1.9mg (quercetin equivalent/1g extract) for ethanol extract, total phenols: 75.9+/-3.7, flavonoids: 31.5+/-2.3 for water extract using Folin-Ciocalteu reagent.
Aslan
Mustafa
M
Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, 06330 Ankara, Turkey. marslan@gazi.edu.tr
Deliorman Orhan
Didem
D
Orhan
Nilüfer
N
Sezik
Ekrem
E
Yesilada
Erdem
E
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000139:chemically induced / Q000188:drug therapy; D000431:Ethanol; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D005951:Glucose Tolerance Test; D005978:Glutathione / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D036562:Helichrysum / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014421:Turkey; D014867:Water
D000975:Antioxidants; D001786:Blood Glucose; D005419:Flavonoids; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D012997:Solvents; D013438:Sulfhydryl Compounds; D014867:Water; D000431:Ethanol; D008315:Malondialdehyde; D005978:Glutathione