Response to hepatocarcinoma Hca-F of mice immunized with heat shock protein 70 from elemene combo tumor cell vaccine.
To analyze immune response to murine hepatocarcinoma Hca-F of mice immunized with heat shock protein 70 (HSP70) derived from elemene combo tumor cell vaccine (EC-TCV) of Hca-F, HSP70 was isolated from EC-TCV by ADP affinity chromatography. Mice were immunized with HSP70 intraperitoneally three times and spleen cells were sampled. For cells, their proliferation and cytotoxicity against Hca-F were measured with MTT assay and their phenotypes were analyzed with flow cytometry. Spleen cells of immunized mice with HSP70 exhibited more potent cytotoxicity against Hca-F and proliferation than that of normal control mice, but less potent than that of mice immunized with EC-TCV. Among three groups, the percent of gammadeltaT lymphocytes in the mice immunized with HSP70 (35.5%) was the highest compared with 6.25% in normal mice, and 28.4% in the mice immunized with EC-TCV. Immunization of HSP70 derived from EC-TCV could elicit potent immune response to Hca-F. HSP70 is one of elements inducing anti-tumor immune responses against Hca-F.
Guo
Lianying
L
Institute for Cancer Biotherapy, Dalian Medical University, Dalian 116027, China.
Shi
Guangxia
G
Gao
Zhihong
Z
Shen
Jie
J
Xing
Rong
R
Qian
Zhenchao
Z
D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000517:prevention & control* / Q000628:therapy; D002453:Cell Cycle / Q000187:drug effects; D050883:HSC70 Heat-Shock Proteins / Q000008:administration & dosage* / Q000276:immunology; D007114:Immunization; D008113:Liver Neoplasms / Q000276:immunology / Q000517:prevention & control* / Q000628:therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D046912:Multiprotein Complexes / Q000008:administration & dosage / Q000276:immunology; D009368:Neoplasm Transplantation; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D015996:Survival Rate
D019496:Cancer Vaccines; D050883:HSC70 Heat-Shock Proteins; D046912:Multiprotein Complexes