Analysis of baicalein, baicalin and wogonin in Scutellariae radix and its preparation by microemulsion electrokinetic chromatography with 1-butyl-3-methylimizolium tetrafluoborate ionic liquid as additive.
Microemulsion electrokinetic chromatography (MEEKC) using 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM-BF4) ionic liquid (IL) as additive was developed for the analysis of baicalin, wogonin and baicalein in Scutellariae radix and its preparation. After conducting a series of optimizations, baseline separation was obtained for the analytes within 5min under the optimum conditions (sodium dodecyl sulfate (SDS) 0.88% (m/v) ethyl acetate 0.8% (v/v) butan-1-ol 0.2% (v/v) and the buffer composition were 25% acetonitrile (v/v), 7.5 mM BMIM-BF4 and 10 mM NaH2PO4, pH 8.2, applied voltage 17.5 kV and detection at 254 nm), the method has been successfully applied to the determination and quantification of the analytes in the extracts of S. radix (cooked), S. radix (raw) and Qingfeiyihuowan which was the preparation including S. radix.
Zhang
Huige
H
Department of Chemistry, Lanzhou University, Lanzhou, China.
Tian
Kan
K
Tang
Jianghong
J
Qi
Shengda
S
Chen
Hongli
H
Chen
Xingguo
X
Hu
Zhide
Z
D001881:Borates / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry; D044950:Flavanones / Q000032:analysis* / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D031364:Scutellaria baicalensis / Q000737:chemistry*
C419324:1-butyl-3-methylimidazolium tetrafluoroborate; D001881:Borates; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D007093:Imidazoles; D010936:Plant Extracts; C038044:baicalin; C006680:baicalein; C085514:wogonin