title: New genus-specific primers for the PCR identification of novel isolates of the genus Streptomonospora.
abstract: The halophilic actinomycete genus Streptomonospora, forming a distinct branch in the 16S rRNA gene phylogenetic tree adjacent to the genera Nocardiopsis and Thermobifida, was first proposed by Cui et al. to accommodate the species type Streptomonospora salina. During a biodiversity and taxonomic study on halophilic filamentous actinomycetes from a saline lake in western China, numerous new halophilic actinomycetes strains were isolated. To confirm whether they are members of the genus Streptomonospora, one set of genus-specific oligonucleotides was designed which allows rapid detection of members of the genus Streptomonospora by means of PCR amplification. The genus specificity of these primers was validated with reference strains as well as with wild-type isolates, which exhibited morphological characteristics common to this genus.
authors.affiliation: Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, People's Republic of China.
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Zhi
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Tang
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Jiang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D002681:China; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D014871:Water Microbiology
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S