title: Paraoxonase gene polymorphism, serum lipid, and oxidized low-density lipoprotein in preeclampsia.
abstract: OBJECTIVE
  Human paraoxonase-1 (PON-1) is thought to play a role in preeclampsia and atherosclerosis, mainly through a reduction in low-density lipoprotein oxidation. Oxidized low-density lipoprotein (LDL) is very important in endothelial dysfunction of preeclampsia. The aim of the present study was to investigate the association between PON1 gene polymorphism and preeclampsia and to determine concentrations of serum lipid in preeclampsia patients. We aimed also to evaluate serum oxidized LDL levels in normal and preeclampsia patients.
  
  
  METHODS
  We performed the present study in 57 control women and 32 preeclampsia patients. PON-1 genotypes were determined by polymerase chain reaction amplification and restriction fragment length polymorphism. Serum triglyceride, cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol levels were measured. We also measured serum levels of oxidized LDL by ELISA method.
  
  
  RESULTS
  There was no significant difference in PON1 genotype frequencies between the control and preeclampsia patients. The levels of serum cholesterol and high-density lipoprotein were significantly lower in preeclampsia patients compared with that of the control women (p=0.05, and p<0.01, respectively). The serum levels of oxidized LDL in preeclampsia patients were significantly higher than those in the control women (p=0.001).
  
  
  CONCLUSIONS
  These findings support the importance of the atherogenic lipid profile and oxidized LDL that is enhanced in preeclampsia, and these findings may be significant contributors to endothelial dysfunction.
authors.affiliation: Department of Obstetrics and Gynecology, Ewha Womans University, Seoul, Republic of Korea. kkyj@ewha.ac.kr
authors.forename: Young Ju
authors.initials: YJ
authors.lastname: Kim
authors.forename: Hyesook
authors.initials: H
authors.lastname: Park
authors.forename: Hwa Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Young-mo
authors.initials: YM
authors.lastname: Ahn
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Ha
authors.forename: Suk Hyo
authors.initials: SH
authors.lastname: Suh
authors.forename: Myung-Geol
authors.initials: MG
authors.lastname: Pang
mesh_terms: D043303:Aryldialkylphosphatase / Q000235:genetics*; D015415:Biomarkers; D005260:Female; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood* / Q000378:metabolism; D010084:Oxidation-Reduction; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000097:blood* / Q000235:genetics*; D011247:Pregnancy
chemical_list: D015415:Biomarkers; D008055:Lipids; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human