title: Diagnosis of gastric syphilis by direct immunofluorescence staining and real-time PCR testing.
abstract: We report on a case of gastric syphilis in a patient with chronic dyspepsia. The diagnosis was established by serology and the demonstration of spirochetes in diffusely inflammed gastric mucosa by staining with a fluorescent monoclonal antibody specific for pathogenic treponemes and by the detection of specific treponemal DNA sequences by a real-time PCR.
authors.affiliation: Division of STD Prevention, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, Mail Stop G-39, 1600 Clifton Road, Atlanta, GA 30333, USA. cyc1@cdc.gov
authors.forename: Cheng-Yen
authors.initials: CY
authors.lastname: Chen
authors.forename: Kai-Hua
authors.initials: KH
authors.lastname: Chi
authors.forename: Robert W
authors.initials: RW
authors.lastname: George
authors.forename: David L
authors.initials: DL
authors.lastname: Cox
authors.forename: Amitabh
authors.initials: A
authors.lastname: Srivastava
authors.forename: Mário
authors.initials: M
authors.lastname: Rui Silva
authors.forename: Fátima
authors.initials: F
authors.lastname: Carneiro
authors.forename: Gregory Y
authors.initials: GY
authors.lastname: Lauwers
authors.forename: Ronald C
authors.initials: RC
authors.lastname: Ballard
mesh_terms: D000328:Adult; D004269:DNA, Bacterial / Q000032:analysis; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D005753:Gastric Mucosa / Q000382:microbiology; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D013272:Stomach Diseases / Q000175:diagnosis* / Q000382:microbiology*; D013587:Syphilis / Q000175:diagnosis* / Q000382:microbiology; D014210:Treponema pallidum / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial