title: Tumoral cell mtDNA approximately 8.9 kb deletion is more common than other deletions in gastric cancer.
abstract: BACKGROUND
  The aim of the study was to clarify the role of deletion of mitochondrial DNA (mtDNA) in gastric carcinogenesis and to determine prevalence of mitochondrial deletions in different regions of tumoral tissue in comparison with adjacent non-tumoral tissue in gastric cancer.
  
  
  METHODS
  In order to investigate whether a high incidence of mutations exists in mtDNA of gastric cancer tissues, we screened five regions of the mitochondrial genome by PCR amplification, Southern blot and DNA sequence analysis.
  
  
  RESULTS
  Of 71 cancer patients, the approximately 8.9 kb deletion was detected among different deletions in 9 cases (12.67%) of the tumoral tissues and 1 case (1.40%) in non-tumoral tissues that were adjacent to the tumors. Level of the 8.9 kb deletion has been found to be more than other deletions in tumoral tissues.
  
  
  CONCLUSIONS
  The approximately 8.9 kb deletion has an obvious correlation with age and histological type. These data suggest that the approximately 8.9 kb deletion in mtDNA may play an important role in gastric carcinogenesis.
authors.affiliation: Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Behnam
authors.initials: B
authors.lastname: Kamalidehghan
authors.forename: Massoud
authors.initials: M
authors.lastname: Houshmand
authors.forename: Mehdi Shafa Shariat
authors.initials: MS
authors.lastname: Panahi
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Abbaszadegan
authors.forename: Patimah
authors.initials: P
authors.lastname: Ismail
authors.forename: Mahnaz Bagher
authors.initials: MB
authors.lastname: Shiroudi
mesh_terms: D000328:Adult; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011230:Precancerous Conditions / Q000235:genetics*; D017384:Sequence Deletion; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm