Preparation and targeted delivery of immunoliposomes bearing poly(ethylene glycol)-coupled humanized anti-hepatoma disulfide-stabilized Fv (hdsFv25) in vitro.
Peptides in bee venom (PBV) have attracted considerable attention for anti-cancer therapy. In this study, sterically stabilized liposomal PBV (PBV-SL) was prepared using Soybean phosphatidylcholine, cholesterol, and the cholesterol derivatives of PEG with terminal COOH groups. The humanized anti-hepatoma disulfide-stabilized Fv (hdsFv25) was coupled to sterically stabilized liposomes. The hdsFv25-immunoliposome has strong affinity and specificity to SMMC-7721 cells in vitro. PBV-loaded sterically stabilized liposomes modified with the hdsFv25 can kill SMMC-7721 cells in vitro with higher efficiency than non-targeted liposomes. These results demonstrate that this strategy should also be applicable to immunotherapy for other cancers.
Hu
Haiyang
H
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, PR China.
Chen
Dawei
D
Liu
Yanfang
Y
Deng
Yihui
Y
Yang
Shoujing
S
Qiao
Mingxi
M
Zhao
Jun
J
Zhao
Xiuli
X
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D001514:Bee Venoms / Q000737:chemistry* / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival; D002784:Cholesterol / Q000737:chemistry; D016503:Drug Delivery Systems; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry* / Q000494:pharmacology; D008081:Liposomes; D008856:Microscopy, Fluorescence; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D013778:Tetrazolium Salts; D013844:Thiazoles
D000970:Antineoplastic Agents; D001514:Bee Venoms; D018796:Immunoconjugates; D008081:Liposomes; D011994:Recombinant Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; C513890:hdsFv25, human; D011092:Polyethylene Glycols; D002784:Cholesterol; C022616:thiazolyl blue