Use of a Plackett-Burman experimental design to examine the impact of extraction parameters on yields and compositions of pectins extracted from chicory roots (Chicorium intybus L.).
Chicory root pectin was isolated by acid extraction followed by alcohol precipitation. Because the extraction conditions have important effects on the features of pectins, an experimental design was used to study the influence of 17 different extraction parameters on yield and composition of pectin: pH, temperature, time of extraction, solid/liquid ratio, and different pretreatments of the pulps before extraction. Twenty extractions were conducted and examined for their significance on yield and sugar content using the Plackett-Burman factorial design. The acid extraction of chicory roots resulted in an average yield of 11% containing 86% of sugars. It was found that extraction temperature, time, protease pretreatment, water purity, and water washing of pulps significantly affected yield and pectin composition with an increase of yield and purity of pectin in harsher extraction conditions.
Robert
Christelle
C
Faculté Universitaire des Sciences Agronomiques de Gembloux, Unité de Chimie Biologique Industrielle, Passage des Déportés 2, B-5030 Gembloux, Belgium. robert.c@fsagx.ac.be
Devillers
Thierry
T
Wathelet
Bernard
B
Van Herck
Jean-Claude
JC
Paquot
Michel
M
D011232:Chemical Precipitation; D018651:Cichorium intybus / Q000737:chemistry*; D000431:Ethanol; D006863:Hydrogen-Ion Concentration; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification*; D010447:Peptide Hydrolases; D018517:Plant Roots / Q000737:chemistry*; D012995:Solubility; D013696:Temperature; D013997:Time Factors
D000431:Ethanol; D010368:Pectins; D010447:Peptide Hydrolases