Commercially available demineralized bone matrix compositions to regenerate calvarial critical-sized bone defects.
BACKGROUND
Demineralized bone matrix products are often used by surgeons to regenerate bone. Several different types of carriers have been combined with demineralized bone matrix to improve clinical handling and surgical outcome. The aim of the study was to quantitate bone regeneration in standard-sized calvarial defects (a critical-sized defect) in response to commercially available demineralized bone matrix formulations.


METHODS
The commercial demineralized bone matrix formulations were tested as received in 8-mm-diameter calvarial critical-sized defects in an athymic rat model. The demineralized bone matrix treatment groups included the following: (1) Allomatrix; (2) demineralized bone matrix plus sodium hyaluronate (DBX); (3) DBX with poly(DL-lactide) mesh; 4) Dynagraft; (5) Grafton; (6) Regenafil; and (7) human demineralized bone matrix without a carrier. An eighth treatment was a poly(DL-lactide) mesh. At designated times of 2, 4, and 8 weeks, the critical-sized defects were recovered and processed for undecalcified histology and histomorphometry. Histomorphometric data were subjected to an analysis of variance and Fisher's protected least significant difference multiple comparison test. Significance was established at p <or= 0.05.


RESULTS
Allomatrix, Dynagraft, Regenafil, and poly(DL-lactide) mesh alone had less bone formation than DBX, Grafton, DBX plus mesh, and demineralized bone matrix.


CONCLUSIONS
DBX, DBX plus mesh, demineralized bone matrix, and Grafton produced more bone formation than Allomatrix, Dynagraft, mesh alone, and Regenafil.
Acarturk
Tahsin Oguz
TO
Pittsburgh, Pa. From the Division of Plastic and Reconstructive Surgery, University of Pittsburgh, and the Bone Tissue Engineering Center, Carnegie Mellon University.
Hollinger
Jeffrey O
JO
D000818:Animals; D001857:Bone Matrix; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000627:therapeutic use*; D004195:Disease Models, Animal; D008297:Male; D051381:Rats; D011923:Rats, Nude; D012886:Skull / Q000293:injuries*; D014947:Wounds and Injuries / Q000601:surgery*
D018786:Bone Substitutes