Human papillomavirus type 33 polymorphisms and high-grade squamous intraepithelial lesions of the uterine cervix.
BACKGROUND
We investigated the association between polymorphisms of human papillomavirus (HPV)-33 and squamous intraepithelial lesions (SILs).


METHODS
Endocervical specimens from 89 women infected with HPV-33, out of a total of 5347 recruited for 2 case-control and 2 cohort studies, were further analyzed by polymerase chain reaction sequencing of the long control region (LCR), E6, and E7.


RESULTS
Of the 89 samples, 64 were normal, 7 had low-grade SILs (including 3 determined by histopathologic analysis), 15 had high-grade SILs (HSILs, including 14 determined by histopathologic analysis), and 3 had an unknown diagnosis. Non-prototype-like LCR variants were significantly associated with HSILs (age- and study site-adjusted odds ratio [OR], 9.2 [95% confidence interval {CI}, 1.8-45.9]). The C7732G variation, which results in the loss of a putative binding site for the cellular upstream stimulatory factor, was associated with HSILs (age- and site-adjusted OR, 8.0 [95% CI, 1.5-42.8]). E6 and E7 polymorphisms were not associated with HSILs. Samples collected at 6-month intervals from 14 participants contained the same variant. The HPV-33 MT 1-0-0 variant carrying the G7584A variation was detected more frequently in women from Brazil (7/20 [35%]) than in women from Canada (1/65 [1.5%]; P=.001).


CONCLUSIONS
Intratypic LCR variants of HPV-33 seem to vary geographically and to differ with respect to their oncogenic potential.
Khouadri
Soraya
S
Laboratoire de Virologie Moléculaire du Centre de Recherche, Hôpital Notre-Dame du Centre Hospitalier de l'Université de Montréal, Montreal (Quebec), Canada.
Villa
Luisa L
LL
Gagnon
Simon
S
Koushik
Anita
A
Richardson
Harriet
H
Ferreira
Silvaneide
S
Tellier
Pierre
P
Simao
João
J
Matlashewski
Greg
G
Roger
Michel
M
Franco
Eduardo L
EL
Coutlée
Francois
F
D016022:Case-Control Studies; D002584:Cervix Uteri / Q000821:virology*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D018307:Neoplasms, Squamous Cell / Q000821:virology*; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics; D030361:Papillomavirus Infections / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000821:virology*
C410917:E7 protein, Human papillomavirus type 33; D009856:Oncogene Proteins, Viral