title: Isolation and characterization of a new T-even bacteriophage, CEV1, and determination of its potential to reduce Escherichia coli O157:H7 levels in sheep.
abstract: Bacteriophage CEV1 was isolated from sheep resistant to Escherichia coli O157:H7 colonization. In vitro, CEV1 efficiently infected E. coli O157:H7 grown both aerobically and anaerobically. In vivo, sheep receiving a single oral dose of CEV1 showed a 2-log-unit reduction in intestinal E. coli O157:H7 levels within 2 days compared to levels in the controls.
authors.affiliation: The Evergreen State College, Olympia, WA 98505, USA.
authors.forename: Raul R
authors.initials: RR
authors.lastname: Raya
authors.forename: Peter
authors.initials: P
authors.lastname: Varey
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Oot
authors.forename: Michael R
authors.initials: MR
authors.lastname: Dyen
authors.forename: Todd R
authors.initials: TR
authors.lastname: Callaway
authors.forename: Tom S
authors.initials: TS
authors.lastname: Edrington
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Kutter
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Brabban
mesh_terms: D000284:Administration, Oral; D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D019453:Escherichia coli O157 / Q000302:isolation & purification / Q000472:pathogenicity / Q000821:virology*; D005260:Female; D005516:Food Microbiology; D008854:Microscopy, Electron; D019936:Probiotics; D012756:Sheep / Q000382:microbiology*; D013604:T-Phages / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure