title: Single laboratory validation of a method for the determination of Bisphenol A, Bisphenol A diglycidyl ether and its derivatives in canned foods by reversed-phase liquid chromatography.
abstract: A method was developed and validated for the simultaneous determination of Bisphenol A (BPA), Bisphenol A diglycidyl ether (BADGE), BADGE-H2O, BADGE-2H2O, BADGE-H2O-HCl, BADGE-HCl, and BADGE-2HCl in canned food using reversed phase high-performance liquid chromatography (HPLC) with fluorescence detection; chromatographic separation of all seven analytes was achieved (Rs > or = 1.08) using HPLC gradient elution technique. Acetonitrile was used to extract the analytes from the food matrix before subjecting the samples to liquid-liquid extraction, solid-phase extraction for further clean-up and preconcentration prior to HPLC analysis. Excellent inter-day precision data (n = 10) and intra-day precision data (n = 5) were obtained on a 200 microg/kg spiked sample. The RSD ranged from 0.20% to 2.96% for the inter-day precision tests, and 0.04% to 2.82% for the intra-day precision tests. Accuracy was measured at three concentration levels: 200, 1000, and 2000 microg/kg; recoveries ranged from 86.07% to 114.06%. The excellent validation data suggests that this method can be applied on canned foods for the determination of migration of BPA, BADGE and its derivatives from can coatings into food.
authors.affiliation: Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
authors.forename: Cuilian
authors.initials: C
authors.lastname: Sun
authors.forename: Lai Peng
authors.initials: LP
authors.lastname: Leong
authors.forename: Philip John
authors.initials: PJ
authors.lastname: Barlow
authors.forename: Sheot Harn
authors.initials: SH
authors.lastname: Chan
authors.forename: Bosco Chen
authors.initials: BC
authors.lastname: Bloodworth
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004852:Epoxy Compounds / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D005519:Food Preservation; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D000098826:Bisphenol A Compounds
chemical_list: D000097:Acetonitriles; D001559:Benzhydryl Compounds; D004852:Epoxy Compounds; D010636:Phenols; C019273:bisphenol A diglycidyl ether; D000098826:Bisphenol A Compounds; C006780:bisphenol A; C032159:acetonitrile