System for determining the relative fitness of multiple bacterial populations without using selective markers.
A device for simultaneously measuring the relative fitness of multiple bacterial populations was developed and evaluated. The new device eliminates the need to construct strains with selectively neutral markers so that strains can be readily distinguished, and it provides a means to perform multispecies competition experiments.
Ahn
Hyo-Jin
HJ
Department of Biological Sciences, Life Sciences South, Rm. 252, University of Idaho, Moscow, ID 83844-3051, USA.
La
Hyun-Joon
HJ
Forney
Larry J
LJ
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods; D015415:Biomarkers; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D007612:Kanamycin / Q000494:pharmacology; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology; D012706:Serratia marcescens / Q000254:growth & development / Q000378:metabolism
D000900:Anti-Bacterial Agents; D015415:Biomarkers; D003470:Culture Media; D007612:Kanamycin; D012293:Rifampin