Nitric oxide in cerebrospinal fluid and local inducible nitric oxide synthase after cauda equina compression in rats.
We investigated the time course of changes in nitric oxide metabolite (NO2- plus NO3-: NOx) levels in the cerebrospinal fluid and the expression of local inducible nitric oxide synthase following cauda equina compression in rats. Cerebrospinal fluid NOx levels were significantly increased from 12 h to 3 days after compression, and decreased thereafter. Histologically, inducible nitric oxide synthase immunoreactivity was observed in macrophages that infiltrated the dura mater on days 1 and 3 after compression, but not in foamy macrophages in the parenchyma of the cauda equina observed afterwards. The pattern of NOx levels coincided with the appearance of inducible nitric oxide synthase labeled macrophages, indicating a critical role of these cells as the main synthesizers of NOx in the acute stage of cauda equina compression.
Wang
Xianjun
X
Division of Orthopedic Surgery, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata University, Niigata-shi, Niigata, Japan.
Kimura
Shinji
S
Kakita
Akiyoshi
A
Hosaka
Noboru
N
Denda
Hiroshi
H
Ito
Takui
T
Hirano
Toru
T
Endo
Naoto
N
D000818:Animals; D002420:Cauda Equina / Q000201:enzymology* / Q000473:pathology; D004195:Disease Models, Animal; D053331:Ectodysplasins / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009408:Nerve Compression Syndromes / Q000134:cerebrospinal fluid* / Q000473:pathology; D009569:Nitric Oxide / Q000134:cerebrospinal fluid*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D009418:S100 Proteins / Q000378:metabolism; D013997:Time Factors
D053331:Ectodysplasins; D009418:S100 Proteins; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II