Candidate mRNAs interacting with fertility protein PUMILIO2 in the human germ line.
Pumilio protein regulates translation of specific mRNAs in morphogenesis and germ-line development of the flies by binding nucleotide motifs GUUGU (A) and AUUGUA (B) in 3'untranslated regions. A human homologue, PUMILIO2 has been recently identified in the germ-line stem cells and the question was raised whether it regulates translation. We designed software to screen the GeneBank for A and B motifs and found that they are not uncommon in the human genome. Moreover, some of the genes containing motifs A and B are germ cell specific, but some others are expressed in a number of other tissues. This may indicate that PUMILIO mediated translational regulation is universally used in developmental processes of the human body.
Spik
Anna
A
Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland.
Oczkowski
Sławomir
S
Olszak
Agata
A
Kotecki
Maciej
M
Formanowicz
Piotr
P
Błazewicz
Jacek
J
Jaruzelska
Jadwiga
J
D001483:Base Sequence; D005854:Germ Cells / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000502:physiology*
C471243:PUM2 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins