title: Intraschisis cavity fluid composition in congenital X-linked retinoschisis.
abstract: OBJECTIVE
  To describe the intraschisis cavity protein composition in congenital X-linked retinoschisis (CXLRS) from two eyes of one child.
  
  
  METHODS
  The authors present a child with complex, Type 3 CXLRS who underwent bilateral surgical repair with autologous plasmin enzyme-assisted lens-sparing vitrectomy. Undiluted samples of intraschisis fluid and vitreous fluid from two eyes (one child) were obtained and used for protein analysis.
  
  
  RESULTS
  The patient underwent successful schisis repair with plasmin-assisted lens-sparing vitrectomy and silicone oil. Two unique protein bands were identified in the intraschisis cavity sample of each eye by gel electrophoresis. The proteins were identified as tenascin-C, an extracellular matrix protein involved in wound healing, and cystatin C, a ubiquitous cysteine protease inhibitor implicated in inflammation.
  
  
  CONCLUSIONS
  Tenascin-C has previously been described in its complex relationship with decorin and fibronectin in normal wound healing. Tenascin's upregulation in sites of inflammation and tenascin's role as an antiadhesive molecule may contribute to the pathogenesis of CXLRS. To the authors' knowledge, this is the first description of intraschisis cavity fluid.
authors.affiliation: Associated Retinal Consultants, William Beaumont Hospital, Royal Oak, Michigan, USA.
authors.forename: Mandar M
authors.initials: MM
authors.lastname: Joshi
authors.forename: Kimberly
authors.initials: K
authors.lastname: Drenser
authors.forename: Michael
authors.initials: M
authors.lastname: Hartzer
authors.forename: Wendy
authors.initials: W
authors.lastname: Dailey
authors.forename: Antonio
authors.initials: A
authors.lastname: Capone
authors.forename: Michael T
authors.initials: MT
authors.lastname: Trese
mesh_terms: D015415:Biomarkers / Q000032:analysis; D055316:Cystatin C; D015891:Cystatins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D005500:Follow-Up Studies; D006801:Humans; D007223:Infant; D008297:Male; D011480:Protease Inhibitors; D012160:Retina / Q000473:pathology; D041441:Retinoschisis / Q000151:congenital / Q000378:metabolism*; D019063:Tenascin / Q000032:analysis*; D041623:Tomography, Optical Coherence; D014821:Vitrectomy / Q000379:methods; D014822:Vitreous Body / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D015415:Biomarkers; C525030:CST3 protein, human; D055316:Cystatin C; D015891:Cystatins; D016326:Extracellular Matrix Proteins; D011480:Protease Inhibitors; D019063:Tenascin