Chemotactic activity of serum obtained from patients with idiopathic dilated cardiomyopathy.
Elevated circulating levels of alpha- and beta-chemokines in heart failure have been reported. The objective of this study was to investigate the interrelation of chemotactic activity of serum and circulating chemokine levels in patients suffering from idiopathic dilated cardiomyopathy (IDCM). Chemokine serum levels (MCP-1, MIP1-alpha, RANTES, IL-8 and TNF-alpha) were determined in patients with IDCM (n = 10), patients with coronary artery disease with normal (CAD-1; n = 10) or depressed (CAD-2; n = 10) left ventricular function and healthy controls (n = 10). The chemotactic effect of sera obtained from these groups was measured using an in vitro chemotaxis assay. Sera obtained from IDCM (5475 +/- 681 cells) showed the highest chemotactic activity when compared to controls (1850 +/- 215 cells), CAD-1 (3325 +/- 275 cells) and CAD-2 (2800 +/- 275 cells, P < 0.05) associated with significantly higher circulating MCP-1 levels. Sera obtained from IDCM patients show a high chemotactic activity associated with significantly elevated circulating MCP-1.
Sigusch
H H
HH
University of Jena, Department of Internal Medicine, Division of Cardiology, 07740 Jena, Germany. Holger.Sigusch@med.uni-jena.de
Lehmann
M H
MH
Reinhardt
D
D
Henke
A
A
Zell
R
R
Leipner
C
C
Figulla
H R
HR
D000328:Adult; D000368:Aged; D002311:Cardiomyopathy, Dilated / Q000097:blood* / Q000150:complications; D018925:Chemokines / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D016207:Cytokines / Q000097:blood; D004452:Echocardiography; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006333:Heart Failure / Q000097:blood / Q000209:etiology; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000097:blood / Q000150:complications
D018925:Chemokines; D016207:Cytokines