[Effects of Guben Hutan Tongmai Recipe on expressions of macrophages and cell adhesion molecules of aortic endothelia in rats with syndrome of phlegm blocking blood vessel].
OBJECTIVE
To observe the effects of Guben Huatan Tongmai Recipe (GBHTTMR), a compound Chinese herbal recipe, on expressions of macrophages and cell adhesion molecules (CAMs) of aortic endothelia in rats with syndrome of phlegm blocking blood vessel, and to explore the pathogenesis of the phlegm-pathogen.


METHODS
Fifty normal male Wistar rats, 7-week in age, were randomly divided into five groups: normal control group, untreated group, high-dose GBHTTMR-treated group, low-dose GBHTTMR-treated group and simvastatin-treated group, with 10 rats in each group. Syndrome of phlegm blocking blood vessel was induced in rats of the latter 4 groups by feeding the rats with high lipid diet. Levels of blood lipid were compared among the 5 groups. The expressions of macrophages and CAMs in aortic endothelia were tested by immunohistochemical staining method.


RESULTS
The level of blood lipid, and the expressions of macrophages and CAMs showed statistical differences between the normal control group and the untreated group (P<0.01), and between the untreated group and the low-, high-dose GBHTTMR-treated and simvastatin-treated groups as well (P<0.05).


CONCLUSIONS
GBHTTMR can decrease the level of serum cholesterol and triglycerides, and increase the level of high density lipoprotein. It also can inhibit the expressions of macrophages, intercellular adhesion molecule-1, vascular cell adhesion molecule-1, P-selectin, and E-selectin.
Wang
Ping
P
Institute of Geratology, Hubei College of Traditional Chinese Medicine, Wuhan, Hubei Province 430061, China. pwang54@yahoo.com.cn
Guo
Fan
F
Liu
Song-Lin
SL
Tian
Dai-Zhi
DZ
Zou
Xiao-Juan
XJ
Cheng
Fang-Ping
FP
D000818:Animals; D001011:Aorta / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002784:Cholesterol / Q000097:blood; D003937:Diagnosis, Differential; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D006937:Hypercholesterolemia / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D000960:Hypolipidemic Agents / Q000494:pharmacology; D007150:Immunohistochemistry; D008055:Lipids / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008516:Medicine, Chinese Traditional; D008517:Phytotherapy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D019821:Simvastatin / Q000494:pharmacology; D013577:Syndrome; D014280:Triglycerides / Q000097:blood
D015815:Cell Adhesion Molecules; D004365:Drugs, Chinese Herbal; D000960:Hypolipidemic Agents; D008055:Lipids; D014280:Triglycerides; C527181:guben hutan tongmai; D002784:Cholesterol; D019821:Simvastatin