title: Helicobacter pylori infection and CagA protein translocation in human primary gastric epithelial cell culture.
abstract: BACKGROUND
  Increasing evidence has shown that Helicobacter pylori CagA protein translocation into gastric epithelial cells plays an important role in the development of gastric inflammation and malignancy. Translocated CagA undergoes tyrosine phosphorylation in gastric adenocarcinoma cell line cells, and CagA involves disruption of cellular apical-junction complex in Madin-Darby canine kidney cells.
  
  
  METHODS
  To elucidate whether these events take place in normal human gastric epithelium, we infected human primary gastric epithelial cells with H. pylori.
  
  
  RESULTS
  Our results demonstrate that CagA protein was translocated into primary gastric epithelial cells and tyrosine phosphorylated. The translocated CagA induces cytoskeletal rearrangement and the disruption of tight junctions in primary gastric epithelial cells.
  
  
  CONCLUSIONS
  This study provides direct evidence of the modulation of gastric epithelial cells by CagA protein translocation, and advances our understanding of the pathogenesis of H. pylori infection.
authors.affiliation: Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
authors.forename: Yo-Ping
authors.initials: YP
authors.lastname: Lai
authors.forename: Jyh-Chin
authors.initials: JC
authors.lastname: Yang
authors.forename: Tzu-Zung
authors.initials: TZ
authors.lastname: Lin
authors.forename: Jaw-Town
authors.initials: JT
authors.lastname: Lin
authors.forename: Jin-Town
authors.initials: JT
authors.lastname: Wang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004285:Dogs; D004847:Epithelial Cells / Q000382:microbiology*; D005753:Gastric Mucosa / Q000382:microbiology*; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D051379:Mice; D010766:Phosphorylation; D021381:Protein Transport; D019108:Tight Junctions / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori