title: Neoplastic transformation and tumorigenesis associated with overexpression of IMUP-1 and IMUP-2 genes in cultured NIH/3T3 mouse fibroblasts.
abstract: Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. To directly elucidate the functional role of IMUP-1 and IMUP-2 on neoplastic transformation and tumorigenicity, we stably transfected IMUP-1 and IMUP-2 into NIH/3T3 mouse fibroblast cells. Cellular characteristics of the neoplastic transformation were assessed by transformation foci, growth in soft agar, and tumor development in nude mice. We found that IMUP-1 and IMUP-2 overexpressing cells showed altered growth properties, anchorage-independent growth in soft agar and inducing tumor in nude mice. Furthermore, IMUP-1 and IMUP-2 transformants proliferated in reduced serum and shortened cell cycle. These results suggest that ectopic overexpression of IMUP-1 and IMUP-2 may play an important role in acquiring a transformed phenotype, tumorigenicity in vivo, and be related to cellular proliferation.
authors.affiliation: School of Life Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea. jaewoong64@hanmail.net <jaewoong64@hanmail.net>
authors.forename: Zae Young
authors.initials: ZY
authors.lastname: Ryoo
authors.forename: Boo Kyoung
authors.initials: BK
authors.lastname: Jung
authors.forename: Sang Ryeul
authors.initials: SR
authors.lastname: Lee
authors.forename: Myoung Ok
authors.initials: MO
authors.lastname: Kim
authors.forename: Sung Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jung Yong
authors.initials: JY
authors.lastname: Ahn
authors.forename: Tae-Hoon
authors.initials: TH
authors.lastname: Lee
authors.forename: Youl Hee
authors.initials: YH
authors.lastname: Cho
authors.forename: Jae Hak
authors.initials: JH
authors.lastname: Park
authors.forename: Jin Kyeoung
authors.initials: JK
authors.lastname: Kim
mesh_terms: D016475:3T3 Cells; D000818:Animals; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D003470:Culture Media; D005260:Female; D005347:Fibroblasts; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors / Q000235:genetics; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D044967:Serum; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D003470:Culture Media; D009687:Nuclear Proteins; D014157:Transcription Factors