Association of matrix metalloproteinase-9 promoter gene polymorphism with chronic periodontitis.
BACKGROUND
Matrix metalloproteinases (MMPs) are related to tissue destruction and remodeling events in periodontal diseases. A single nucleotide polymorphism in the promoter region of human MMP-9 gene is associated with the risk of some inflammatory diseases. Therefore, the aim of this study was to investigate the association between MMP-9 promoter polymorphism and severe generalized chronic periodontitis in a Turkish population.


METHODS
Samples of venous blood and DNA were obtained from 70 severe generalized chronic periodontitis patients and 70 healthy subjects. The alleles of the C/T polymorphism at position -1562 in the promoter region of the MMP-9 gene were distinguished by cutting with the SphI restriction enzyme. Genotype and allele frequencies were calculated, and data were analyzed by the chi2 test.


RESULTS
There was a significant difference in MMP-9 genotypes between chronic periodontitis patients and healthy controls. The odds ratios for the CT genotype and the combination of CT and TT genotypes were 0.4 (95% confidence interval, 0.17 to 0.93; P=0.02) and 0.37 (95% confidence interval, 016 to 0.85; P=0.01) relative to the subjects with the CC genotype, respectively.


CONCLUSIONS
MMP-9 promoter gene polymorphism seems to be associated with severe generalized chronic periodontitis.
Keles
Gonca Cayir
GC
Department of Periodontology, Faculty of Dentistry, Ondokuzmayis University, Samsun, Turkey. goncakeles@hotmail.com
Gunes
Sezgin
S
Sumer
A Pinar
AP
Sumer
Mahmut
M
Kara
Nurten
N
Bagci
Hasan
H
Koprulu
Hulya
H
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002908:Chronic Disease; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000201:enzymology / Q000235:genetics; D010518:Periodontitis / Q000201:enzymology* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
D020780:Matrix Metalloproteinase 9