Electron paramagnetic resonance studies of an integral membrane peptide inserted into aligned phospholipid bilayer nanotube arrays.
This communication reports for the first time the determination of the helical tilt of an integral membrane peptide inserted into aligned phospholipids bilayer nanotube arrays using spin label EPR spectroscopy. Also, we demonstrate herein how the helical tilt of the peptide can be easily calculated using the hyperfine splitting values gleaned from a perpendicularly aligned bilayer in phospholipid bilayer nanotube arrays. EPR spectral simulations were used to verify the method.
Karp
Ethan S
ES
Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio 45056, USA.
Inbaraj
Johnson J
JJ
Laryukhin
Mikhail
M
Lorigan
Gary A
GA
D003497:Cyclic N-Oxides / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011950:Receptors, Cholinergic / Q000737:chemistry*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D008051:Lipid Bilayers; D011950:Receptors, Cholinergic; D013113:Spin Labels; D004134:Dimyristoylphosphatidylcholine; C037109:2,2,6,6-tetramethylpiperidine-N-oxide-4-amino-4-carboxylic acid