Overview of DNA purification for nucleic acid-based diagnostics from environmental and clinical samples.
Direct deoxyribonucleic acid (DNA)-based detection methods are crucial for future environmental monitoring and clinical diagnosis. In this chapter, we provide an overview of the various sample preparation approaches for bacteria for direct analyses (i.e., without culturing) in environmental and clinical samples. The issues of sampling, sample preservation, separation of the microorganisms from the environmental or clinical matrix, and DNA purification are covered. This chapter will focus on the advantages and the disadvantages of the methods available.
Rudi
Knut
K
Matforsk AS, Norwegian Food Research Institute, Oslo, Norway.
Jakobsen
Kjetill S
KS
D000818:Animals; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D004783:Environmental Microbiology; D006801:Humans; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial