title: Nuclear and mitochondrial localization of the putative RNA helicase DHX32.
abstract: RNA helicases are involved in all aspects of RNA metabolism. We identified DHX32 as a novel gene with homology to the DHX family of RNA helicases. To gain insight into the role of DHX32 in RNA metabolism, we determined its subcellular localization. Electron microscopy immunocytochemistry detected DHX32 in the nucleus and mitochondria of the myeloid leukemia cell line HL-60. Confocal microscopy shows mitochondrial localization of DHX32 in HeLa cells. Double labeling showed close proximity of DHX32 to some of the newly synthesized RNA in the mitochondria. These results suggest that DHX32 is a putative RNA helicase that might be involved in regulating nuclear and mitochondrial gene expression.
authors.affiliation: Division of Haematopathology, Department of Paediatric Laboratory Medicine, Hospital for Sick Children, Toronto, Canada.
authors.forename: Zaman
authors.initials: Z
authors.lastname: Alli
authors.forename: Cameron
authors.initials: C
authors.lastname: Ackerley
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Bandar
authors.initials: B
authors.lastname: Al-Saud
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdelhaleem
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D021381:Protein Transport; D012313:RNA / Q000648:ultrastructure; D000077278:RNA, Mitochondrial
chemical_list: D000077278:RNA, Mitochondrial; D012313:RNA; C466365:DHX32 protein, human; D053487:DEAD-box RNA Helicases