FRET templated by G-quadruplex DNA: a specific ternary interaction using an original pair of donor/acceptor partners.
G-quadruplex represents a suitable scaffold for FRET (fluorescence resonance energy transfer) since its two external quartets offer two well-defined binding sites for concomitant trapping of donor/acceptor partners. Combining selective G-quadruplex binders (macrocyclic bis(quinacridine) BOQ(1) or monomeric quinacridine MMQ(1), donor) with a highly fluorescent DNA probe (thiazole orange, acceptor), we designed a structure-specific FRET-system based on an unprecedented noncovalent ternary complex. This system could be potentially usable as a signature for quadruplex-DNA conformation in solution, but also might offer a unique means for observing cation and ligand binding influence on quadruplex topology.
Allain
Clémence
C
Laboratoire de Chimie des Interactions Moléculaires, Collège de France, CNRS UPR 285, 11 place Marcelin Berthelot, 75005 Paris, France.
Monchaud
David
D
Teulade-Fichou
Marie-Paule
MP
D000166:Acridines / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry*; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D009690:Nucleic Acid Conformation; D011804:Quinolines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000166:Acridines; D052160:Benzothiazoles; D015342:DNA Probes; D005456:Fluorescent Dyes; D011804:Quinolines; C051000:thiazole orange; D004247:DNA