Caveolin-1 polarization in migrating endothelial cells is directed by substrate topology not chemoattractant gradient.
Polarization is a hallmark of migrating cells, and an asymmetric distribution of proteins is essential to the migration process. Caveolin-1 is highly polarized in migrating endothelial cells (EC). Several studies have shown caveolin-1 accumulation in the front of migrating EC while others report its accumulation in the EC rear. In this paper we address these conflicting results on polarized localization of caveolin-1. We find evidence for the hypothesis that different modes of locomotion lead to differences in protein polarization. In particular, we show that caveolin-1 is primarily localized in the rear of cells migrating on a planar substrate, but in the front of cells traversing a three-dimensional pore. We also show that a chemoattractant, present either as a gradient or ubiquitously in the medium, does not alter caveolin-1 localization in cells in either mode of locomotion. Thus we conclude that substrate topology, and not the presence of a chemoattractant, directs the polarization of caveolin-1 in motile ECs.
Santilman
Virginie
V
Department of Anesthesiology Research, Cleveland Clinic, Cleveland, Ohio 44195, USA.
Baran
JoAnne
J
Anand-Apte
Bela
B
Fox
Paul L
PL
Parat
Marie-Odile
MO
D000818:Animals; D001011:Aorta / Q000502:physiology; D002417:Cattle; D051242:Caveolin 1 / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D016764:Cell Polarity / Q000502:physiology*; D002633:Chemotaxis / Q000502:physiology; D042783:Endothelial Cells / Q000502:physiology*; D006801:Humans; D042442:Vascular Endothelial Growth Factors / Q000502:physiology
C495540:CAV1 protein, human; D051242:Caveolin 1; D042442:Vascular Endothelial Growth Factors