Using bicellar mixtures to form supported and suspended lipid bilayers on silicon chips.
Bicellar mixtures, planar lipid bilayer assemblies comprising long- and short-chain phosphatidylcholine lipids in suspension, were used to form supported lipid bilayers on flat silicon substrate and on nanotextured silicon substrates containing arrays of parallel troughs (170 nm wide, 380 nm deep, and 300 nm apart). Confocal fluorescence and atomic force microscopies were used to characterize the resulting lipid bilayer. Formation of a continuous biphasic undulating lipid bilayer membrane, where the crests and troughs corresponded to supported and suspended lipid bilayer regions, is demonstrated. The use of interferometric lithography to fabricate nanotexured substrates provides an advantage over other nanotextured substrates such as nanoporous alumina by offering flexibility in designing different geometries for suspending lipid bilayers.
Zeineldin
Reema
R
Center for Biomedical Engineering and Department of Chemical & Nuclear Engineering, University of New Mexico, Albuquerque, New Mexico 87131, USA.
Last
Julie A
JA
Slade
Andrea L
AL
Ista
Linnea K
LK
Bisong
Paul
P
O'Brien
Michael J
MJ
Brueck
S R J
SR
Sasaki
Darryl Y
DY
Lopez
Gabriel P
GP
D008051:Lipid Bilayers; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D036103:Nanotechnology; D010713:Phosphatidylcholines / Q000737:chemistry; D016062:Porosity; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D012825:Silicon