title: Effect of methyl jasmonate on phenolics, isothiocyanate, and metabolic enzymes in radish sprout (Raphanus sativus L.).
abstract: The effect of spraying exogenous plant hormone methyl jasmonate (MeJA) upon radish sprout (Raphanus sativus L.) was investigated in aspects of total phenolic content (TPC), isothiocyanate content, antioxidant activity of the radish extract, and enzymatic activities of phenylalanine ammonia lyase (PAL) and myrosinase. The MeJA treatment significantly increased the TPC that resulted in the increased DPPH* (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capacity. In addition, the PAL activity also increased by 60% at 24 h after MeJA treatment. However, the same treatment decreased the amount of 4-methylthio-3-butenylisothiocyanate (MTBITC), a major isothiocyanate in radish sprout and the activity of myrosinase, an enzyme related to produce isothiocyanates.
authors.affiliation: Department of Food Science and Human Nutrition, Clemson University, Clemson, South Carolina 29634, USA. hjkim7923@hotmail.com
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.forename: Ju-Hee
authors.initials: JH
authors.lastname: Choi
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis; D001713:Biphenyl Compounds; D003517:Cyclopentanes / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000378:metabolism; D017879:Isothiocyanates / Q000032:analysis*; D009753:Nutritive Value; D054883:Oxylipins; D010636:Phenols / Q000032:analysis*; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D031224:Raphanus / Q000737:chemistry* / Q000187:drug effects* / Q000201:enzymology
chemical_list: D000085:Acetates; D000975:Antioxidants; D001713:Biphenyl Compounds; D003517:Cyclopentanes; D016166:Free Radical Scavengers; D017879:Isothiocyanates; D054883:Oxylipins; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D010937:Plant Growth Regulators; C037152:isothiocyanic acid; C072239:methyl jasmonate; C004931:1,1-diphenyl-2-picrylhydrazyl; D006026:Glycoside Hydrolases; C022739:thioglucosidase; D010650:Phenylalanine Ammonia-Lyase