title: FTMS and TOF/TOF mass spectrometry in concert: identifying peptides with high reliability using matrix prespotted MALDI target plates.
abstract: In this paper we describe a combination of the mass spectrometric techniques MALDI-TOF/TOF and MALDI-FTMS to identify proteins in complex samples using prespotted MALDI target plates. By this procedure accurate FTMS mass measurements and TOF/TOF data are obtained from the same spot. We have found that this combination of techniques leads to more reliable identification of peptides.
authors.affiliation: Laboratory for Neuro-Oncology, Department of Neurology, Room AE312, Erasmus Medical Center, P.O. Box 1738, 3000DR Rotterdam, The Netherlands.
authors.forename: Lennard J
authors.initials: LJ
authors.lastname: Dekker
authors.forename: Peter C
authors.initials: PC
authors.lastname: Burgers
authors.forename: Coşkun
authors.initials: C
authors.lastname: Guzel
authors.forename: Theo M
authors.initials: TM
authors.lastname: Luider
mesh_terms: D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000097:blood* / Q000737:chemistry; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001798:Blood Proteins; D010455:Peptides; D020543:Proteome