title: Identification of single-nucleotide polymorphisms in 5' end and exons of the PRKAG3 gene in Hubbard White broiler, Leghorn layer, and three Chinese indigenous chicken breeds.
abstract: 5'-AMP-activated protein kinase plays an important role in regulating the level of ATP in the presence of metabolic stress. Previous studies revealed that polymorphisms in 5'-AMP-activated protein kinase gamma 3 subunit (PRKAG3) gene are associated with meat quality in pigs. In the present study, single-nucleotide polymorphisms (SNPs) in the 5'-end and exons of chicken PRKAG3 gene were identified with the method of single-strand conformation polymorphism in Hubbard ISA White broiler, Leghorn layer, and three Chinese indigenous chicken breeds, Tibet Chicken, Shouguang Chicken and Beijing Yellow Chicken. Two SNPs in the 5'-end of the gene and 10 SNPs in exons 3, 4, 9 and 11, of which three caused amino acid substitutions, were identified in the PRKAG3 gene of the five chicken breeds. The results will facilitate further study on the association between the mutations of PRKAG3 and chicken meat quality.
authors.affiliation: Key Laboratories of Animal Breeding and Genetics of Ministry of Agriculture of P.R. China.
authors.forename: C J
authors.initials: CJ
authors.lastname: Zhao
authors.forename: C F
authors.initials: CF
authors.lastname: Wang
authors.forename: X M
authors.initials: XM
authors.lastname: Deng
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: Ch
authors.initials: Ch
authors.lastname: Wu
mesh_terms: D024506:5' Flanking Region; D055372:AMP-Activated Protein Kinases; D000818:Animals; D002645:Chickens / Q000235:genetics*; D005091:Exons; D005787:Gene Frequency; D008460:Meat; D009097:Multienzyme Complexes / Q000235:genetics*; D020125:Mutation, Missense; D020641:Polymorphism, Single Nucleotide; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D021122:Protein Subunits / Q000235:genetics
chemical_list: D009097:Multienzyme Complexes; D021122:Protein Subunits; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases