Molecular cloning and function of Oct-3 isoforms in cynomolgus monkey embryonic stem cells.
Oct-3 is a key molecule for maintaining self-renewal in mouse embryonic stem (ES) cells. The function of Oct-3 in ES cells of other species, however, especially primate ES cells, is not clear. In the present study, we cloned two splicing isoforms of Oct-3, Oct-3A and Oct-3B, from cynomolgus monkey ES cells, and found that they have high homology to human Oct-3A and Oct-3B. To examine their function, Oct-3A and Oct-3B were overexpressed in cynomolgus monkey ES cells. Transient Oct-3A overexpression induced ES cell differentiation into endodermal and mesodermal lineages and disrupted proliferation of undifferentiated monkey ES cells. In contrast, Oct-3B overexpression did not induce differentiation of monkey ES cells. These findings indicate that a certain Oct-3A expression level has an important role in sustaining self-renewal in non-human primate ES cells.
Fujimoto
Yasuko
Y
Laboratory of Embryonic Stem Cell Research, Stem Cell Research Center and Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, 606-8507, Japan.
Hasegawa
Kouichi
K
Suemori
Hirofumi
H
Ito
Juichi
J
Nakatsuji
Norio
N
D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003114:Colony-Forming Units Assay; D004622:Embryo, Mammalian / Q000166:cytology*; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D008252:Macaca fascicularis / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D050814:Octamer Transcription Factor-3 / Q000737:chemistry / Q000378:metabolism*; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D014162:Transfection
D015415:Biomarkers; D050814:Octamer Transcription Factor-3; D020033:Protein Isoforms