Crystallization and preliminary crystallographic analysis of a novel plant type III polyketide synthase that produces pentaketide chromone.
Pentaketide chromone synthase (PCS) from Aloe arborescens is a novel plant-specific type III polyketide synthase that catalyzes the formation of 5,7-dihydroxy-2-methylchromone from five molecules of malonyl-CoA. Recombinant PCS expressed in Escherichia coli was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group P2(1), with unit-cell parameters a = 73.2, b = 88.4, c = 70.0 A, alpha = gamma = 90.0, beta = 95.6 degrees . Diffraction data were collected to 1.6 A resolution using synchrotron radiation at BL24XU of SPring-8.
Morita
Hiroyuki
H
Mitsubishi Kagaku Institute of Life Sciences (MITILS), 11 Minamiooya, Machida, Tokyo 194-8511, Japan.
Kondo
Shin
S
Abe
Tsuyoshi
T
Noguchi
Hiroshi
H
Sugio
Shigetoshi
S
Abe
Ikuro
I
Kohno
Toshiyuki
T
D000504:Aloe / Q000201:enzymology*; D002867:Chromones / Q000737:chemistry / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D010940:Plant Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D048630:Polyketide Synthases / Q000737:chemistry* / Q000145:classification / Q000235:genetics
D002867:Chromones; D010940:Plant Proteins; D048630:Polyketide Synthases