Solvent-dependent structure of two tryptophan-rich antimicrobial peptides and their analogs studied by FTIR and CD spectroscopy.
Structural changes for a series of antimicrobial peptides in various solvents were investigated by a combined approach of FTIR and CD spectroscopy. The well-characterized and potent antimicrobial peptides indolicidin and tritrpticin were studied along with several analogs of tritrpticin, including Tritrp1 (amidated analog of tritrpticin), Tritrp2 (analog of Tritrp1 with Arg-->Lys substitutions), Tritrp3 (analog of Tritrp1 with Pro-->Ala substitutions) and Tritrp4 (analog of Tritrp1 with Trp-->Tyr substitutions). All peptides were studied in aqueous buffer, ethanol and in the presence of dodecylphosphocholine (DPC) micelles. It was shown that tritrpticin and its analogs preferentially adopt turn structures in all solvents studied. The turn structures formed by the tritrpticin analogs bound to DPC micelles are more compact and more conformationally restricted compared to indolicidin. While several peptides showed a slight propensity for an alpha-helical conformation in ethanol, this trend was only strong for Tritrp3, which also adopted a largely alpha-helical structure with DPC micelles. Tritrp3 also demonstrated along with Tritrp1 the highest ability to interact with DPC micelles, while Tritrp2 and Tritrp4 showed the weakest interaction.
Andrushchenko
Valery V
VV
Department of Biological Sciences, University of Calgary, 2500 University Dr, NW, Calgary, Alberta, Canada T2N 1N4.
Vogel
Hans J
HJ
Prenner
Elmar J
EJ
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D002021:Buffers; D002942:Circular Dichroism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry / Q000494:pharmacology; D008823:Micelles; D009842:Oligopeptides / Q000737:chemistry*; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared; D014270:Trifluoroethanol / Q000737:chemistry / Q000494:pharmacology
D023181:Antimicrobial Cationic Peptides; D002021:Buffers; D008823:Micelles; D009842:Oligopeptides; D012997:Solvents; C402895:tritrpticin; C073143:indolicidin; D010767:Phosphorylcholine; D000431:Ethanol; C028810:dodecylphosphocholine; D014270:Trifluoroethanol; D004121:Dimethyl Sulfoxide