Relevance of influenza a virus detection by PCR, shell vial assay, and tube cell culture to rapid reporting procedures.
Influenza A virus was detected at higher rates and for more extended time periods with real-time PCR than with cell cultures. We show here that, using the theranostic approach, rapid viral detection and reporting can provide for early implementation and assessment of available antiviral therapy.
Zitterkopf
Nicole L
NL
Division of Clinical Microbiology, Mayo Clinic, 200 1st St., SW, Rochester, MN 55905, USA.
Leekha
Surbhi
S
Espy
Mark J
MJ
Wood
Christina M
CM
Sampathkumar
Priya
P
Smith
Thomas F
TF
D000293:Adolescent; D000328:Adult; D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014776:Virus Cultivation / Q000295:instrumentation / Q000379:methods
D004279:DNA, Viral