Simultaneous visualization of spatial and chromatic aberrations by two-dimensional Fourier transform spectral interferometry.
We demonstrate the use of a simple tool to simultaneously visualize and characterize chromatic and spherical aberrations that are present in multiphoton microscopy. Using two-dimensional Fourier transform spectral interferometry, we measured these aberrations, deducing in a single shot spatiotemporal effects in high-numerical-aperture objectives.
Amir
W
W
Physics, Department of Colorado School of Mines, Golden, Colorado 80402, USA. wamir@mines.edu
Planchon
T A
TA
Durfee
C G
CG
Squier
J A
JA
Gabolde
P
P
Trebino
R
R
Müller
M
M
D000465:Algorithms; D016477:Artifacts; D005583:Fourier Analysis; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007368:Interferometry / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods