A high-yield one-step purification method using copper-chelating chromatography for recombinant proteins fused with maltose-binding protein.
Lee
Ka-Ming
KM
Molecular Biotechnology Program, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Ma
Kit-Wan
KW
Shaw
Pang-Chui
PC
Wong
Kam-Bo
KB
D002352:Carrier Proteins / Q000302:isolation & purification*; D002614:Chelating Agents / Q000737:chemistry*; D002845:Chromatography / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D058619:Maltose-Binding Proteins; D010750:Phosphoproteins / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification*; D012269:Ribosomal Proteins / Q000302:isolation & purification; D012685:Sepharose / Q000737:chemistry
D002352:Carrier Proteins; D002614:Chelating Agents; D058619:Maltose-Binding Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; C031937:phosphoprotein P2, ribosomal; D003300:Copper; D012685:Sepharose