Chemical reaction at specific sites and reaction-induced self-assembly as observed by in situ and real time SANS: enzymatic polymerization to synthetic cellulose.
We have investigated the self-assembling process of cellulose artificially synthesized via enzymatic polymerization by means of in situ and time-resolved SANS (small-angle neutron scattering). The results elucidated the following: (i) Cellulose molecules synthesized at a special reaction site of the enzyme (cellulase) located on or near the smooth surface of self-assembled enzymes formed in the reaction medium. (ii) The synthesized molecules associated themselves via DLA (diffusion-limited association) and crystallized into fibrils. (iii) The fibrils formed the aggregates, which had surface fractal dimension D(s) increasing from 2 to 2.3 with the reaction time, on the smooth surface of the enzyme aggregates.
Hashimoto
Takeji
T
Advanced Science Research Center, Japan Atomic Energy Agency, Tokai, Ibaraki 319-1195, Japan.
Tanaka
Hirokazu
H
Koizumi
Satoshi
S
Kurosaki
Kazuhiro
K
Ohmae
Masashi
M
Kobayashi
Shiro
S
D001672:Biocompatible Materials / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000737:chemistry; D002482:Cellulose / Q000138:chemical synthesis* / Q000737:chemistry*; D055598:Chemical Phenomena; D002625:Chemistry, Organic / Q000379:methods; D002627:Chemistry, Physical; D004058:Diffusion; D017709:Fractals; D046911:Macromolecular Substances; D009502:Neutrons; D011108:Polymers / Q000737:chemistry; D012542:Scattering, Radiation; D012997:Solvents / Q000737:chemistry; D013499:Surface Properties
D001672:Biocompatible Materials; D046911:Macromolecular Substances; D011108:Polymers; D012997:Solvents; D002482:Cellulose; D002480:Cellulase