Proteome comparison following self- and across-pollination in self-incompatible apricot (Prunus armeniaca L.).
The study compared the protein differences between self- and across-pollinated self-incompatible (SI) apricots by two-dimensional gel electrophoresis and liquid chromatography-electrospray ion trap tandem mass spectrometry, the results showed that nine protein spots were expressed in self-pollinated pistil and only one was expressed in cross-pollinated pistils. Sixteen and three protein spots were up- and down-regulated in cross-pollinated pistils, respectively, compared with self-pollinated pistils. Seven protein spots were identified unambiguously by SEQUEST in NCBI protein database: Actin-12, enolase, MYB transcription-factor-like protein, heat-shock protein 70 were upregulated in cross-pollinated pistils compared with self-pollinated pistils; and actin-7, actin-8 and fructose bisphosphate aldolase-like protein were detected only in self-pollinated pistils.
Feng
Jianrong
J
Horticultural Science and Engineering College, Shandong Agricultural University, Daizong Street 61, Taian, 271018, Shandong, China.
Chen
Xuesen
X
Yuan
Zhaohe
Z
He
Tianming
T
Zhang
Lijie
L
Wu
Yan
Y
Liu
Wen
W
Liang
Qing
Q
D000595:Amino Acid Sequence; D002853:Chromatography, Liquid / Q000379:methods; D035264:Flowers / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000378:metabolism*; D011058:Pollen / Q000502:physiology*; D020543:Proteome / Q000378:metabolism*; D027861:Prunus / Q000235:genetics*; D053719:Tandem Mass Spectrometry / Q000379:methods
D010940:Plant Proteins; D020543:Proteome