Cold shock domain family members YB-1 and MSY4 share essential functions during murine embryogenesis.
Three cold shock domain (CSD) family members (YB-1, MSY2, and MSY4) exist in vertebrate species ranging from frogs to humans. YB-1 is expressed throughout embryogenesis and is ubiquitously expressed in adult animals; it protects cells from senescence during periods of proliferative stress. YB-1-deficient embryos die unexpectedly late in embryogenesis (embryonic day 18.5 [E18.5] to postnatal day 1) with a runting phenotype. We have now determined that MSY4, but not MSY2, is also expressed during embryogenesis; its abundance declines substantially from E9.5 to E17.5 and is undetectable on postnatal day 1(adult mice express MSY4 in testes only). Whole-mount analysis revealed similar patterns of YB-1 and MSY4 RNA expression in E11.5 embryos. To determine whether MSY4 delays the death of YB-1-deficient embryos, we created and analyzed MSY4-deficient mice and then generated YB-1 and MSY4 double-knockout embryos. MSY4 is dispensable for normal development and survival, but the testes of adult mice have excessive spermatocyte apoptosis and seminiferous tubule degeneration. Embryos doubly deficient for YB-1 and MSY4 are severely runted and die much earlier (E8.5 to E11.5) than YB-1-deficient embryos, suggesting that MSY4 indeed shares critical cellular functions with YB-1 in the embryonic tissues where they are coexpressed.
Lu
Zhi Hong
ZH
Division of Oncology, Department of Medicine, Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO 63110-1093, USA.
Books
Jason T
JT
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Timothy J
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D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D047108:Embryonic Development; D005260:Female; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism*; D012671:Seminiferous Tubules / Q000503:physiopathology; D013090:Spermatocytes / Q000502:physiology; D014018:Tissue Distribution; D014157:Transcription Factors
C408323:Csda protein, mouse; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D014157:Transcription Factors