title: Biochemical and phylogenetic characterization of a novel terrestrial hyperthermophilic archaeon pertaining to the genus Pyrococcus from an Algerian hydrothermal hot spring.
abstract: A hyperthermophilic anaerobic archeon, strain HT3, was isolated from hydrothermal hot spring in Northeast Algeria. The strain is a regular coccus, highly motile, obligatory anaerobic, heterotrophic. It utilizes proteinaceous complex media (peptone, tryptone or yeast extract). Sulfur is reduced to Hydrogen sulfide and enhances growth. It shares with other Pyrococcus species the heterotrophic mode of nutrition, the hyperthermophily, the ability to utilize amino acids as sole carbon and nitrogen sources and the ether lipid composition. The optimal growth occurs at 80-85 degrees C, pH 7.5 and 1.5% NaCl. The G + C content was 43 mol%. Considering its morphology, physiological properties, nutritional features and phylogenetic analyses based on 16S rRNA gene sequencing, this strain is described as a new terrestrial isolate pertaining to the genus Pyrococcus.
authors.affiliation: Laboratoire de Microbiologie Industrielle, URCA Université de Reims Champagne Ardenne, Moulin de la Housse, BP 1039, 51687, Reims Cedex 02, France. kmkmsetif@yahoo.fr
authors.forename: Mouloud
authors.initials: M
authors.lastname: Kecha
authors.forename: Said
authors.initials: S
authors.lastname: Benallaoua
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Touzel
authors.forename: Roger
authors.initials: R
authors.lastname: Bonaly
authors.forename: Francis
authors.initials: F
authors.lastname: Duchiron
mesh_terms: D000462:Algeria; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001482:Base Composition; D019641:DNA, Archaeal / Q000032:analysis; D030541:Databases, Genetic; D005995:Glyceryl Ethers / Q000032:analysis; D045482:Hot Springs / Q000382:microbiology*; D006862:Hydrogen Sulfide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010802:Phylogeny; D011506:Proteins / Q000378:metabolism; D019711:Pyrococcus / Q000737:chemistry / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism; D013696:Temperature; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D019641:DNA, Archaeal; D005995:Glyceryl Ethers; D011506:Proteins; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D013455:Sulfur; D010100:Oxygen; D006862:Hydrogen Sulfide