title: Geranylgeranyl-pyrophosphate (GGPP) synthase is down-regulated during differentiation of osteoblastic cell line MC3T3-E1.
abstract: Isoprenylation of geranylgeranyl-pyrophosphate (GGPP) is critical for activation of small GTPases. We examined the roles of GGPP synthase (GGPPS) during the differentiation induced by the cell-to-cell contact in osteoblastic cell line MC3T3-E1 cells. We found that (1) both mRNA and protein expression of GGPPS was reduced with decrement of its activity during the differentiation, (2) GGOH, which is converted to GGPP in the cells, inhibited differentiation. These results suggest that the decrement of GGPP is critical for the cell-to-cell contact-induced differentiation, in which the down-regulation of GGPPS might be involved.
authors.affiliation: Department of Clinical Cell Biology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chiba-city, Chiba 260-8670, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Yoshida
authors.forename: Mie
authors.initials: M
authors.lastname: Asanuma
authors.forename: Laura
authors.initials: L
authors.lastname: Grossmann
authors.forename: Masami
authors.initials: M
authors.lastname: Fuse
authors.forename: Takahisa
authors.initials: T
authors.lastname: Shibata
authors.forename: Taeko
authors.initials: T
authors.lastname: Yonekawa
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Koichi
authors.initials: K
authors.lastname: Ueno
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Yasuda
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Saito
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tatsuno
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D015536:Down-Regulation / Q000502:physiology*; D004789:Enzyme Activation / Q000502:physiology; D051231:Farnesyltranstransferase / Q000096:biosynthesis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D051379:Mice; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: C495428:Ggps1 protein, mouse; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D051231:Farnesyltranstransferase; D020559:Monomeric GTP-Binding Proteins