title: Identification of 14-3-3epsilon substrates from embryonic murine brain.
abstract: Mice deficient in 14-3-3epsilon exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3epsilon interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3epsilon and for maintaining USP8 in the cytosol.
authors.affiliation: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA.
authors.forename: Bryan A
authors.initials: BA
authors.lastname: Ballif
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Cao
authors.forename: Daniel
authors.initials: D
authors.lastname: Schwartz
authors.forename: Kermit L
authors.initials: KL
authors.lastname: Carraway
authors.forename: Steven P
authors.initials: SP
authors.lastname: Gygi
mesh_terms: D048948:14-3-3 Proteins / Q000032:analysis / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000196:embryology* / Q000378:metabolism*; D019556:COS Cells; D002522:Chlorocebus aethiops; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D056827:Endosomal Sorting Complexes Required for Transport; D013058:Mass Spectrometry; D051379:Mice; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010766:Phosphorylation; D043222:Ubiquitin Thiolesterase
chemical_list: D048948:14-3-3 Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D010450:Endopeptidases; D043222:Ubiquitin Thiolesterase; C541536:Usp8 protein, mouse; C091939:ubiquitin isopeptidase