title: The effect of topical doxycycline usage on gingival crevicular fluid MMP-8 levels of chronic and aggressive periodontitis patients: a pilot study.
abstract: The aim of this study was to evaluate the efficacy of topical subgingival application of doxycycline hyclate (DH) gel adjunctive to non-surgical periodontal therapy on gingival crevicular fluid (GCF) matrix metalloproteinase (MMP)-8 levels in chronic and aggressive periodontitis patients. Forty teeth of 10 chronic periodontitis patients and 32 teeth of eight aggressive periodontitis patients were screened for 6 months. Scaling and root planing (SRP) was applied to the control sites and DH gel adjunctive to SRP was applied to the test sites of each patient simultaneously. GCF MMP-8 levels were analysed at baseline, 7 days; and at 1, 3 and 6 months by Sandwich Elisa Method. At 1, 3 and 6 months, probing depth (P < 0.0051) and plaque scores and bleeding on probing values (P = 0.000) significantly decreased in each group when compared with the baseline, but there was no statistically significant difference between the test and control sites. GCF MMP-8 levels reduced presenting statistically significant differences on 7 days, 1, 3 and 6 months in four of the groups (P < 0.05); however, intergroup differences were not statistically significant. Developing functional and immunological-based chair-side MMP tests might serve as useful adjunctive diagnostic tools when monitoring the effects of DH gel application.
authors.affiliation: Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Ağan
authors.forename: S
authors.initials: S
authors.lastname: Sönmez
authors.forename: M
authors.initials: M
authors.lastname: Serdar
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage*; D002908:Chronic Disease; D003692:Delayed-Action Preparations; D012534:Dental Scaling; D004318:Doxycycline / Q000008:administration & dosage / Q000031:analogs & derivatives*; D005260:Female; D005782:Gels; D005883:Gingival Crevicular Fluid / Q000201:enzymology; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000032:analysis; D008875:Middle Aged; D010518:Periodontitis / Q000188:drug therapy* / Q000201:enzymology; D010865:Pilot Projects; D016037:Single-Blind Method; D018709:Statistics, Nonparametric
chemical_list: D000891:Anti-Infective Agents, Local; D003692:Delayed-Action Preparations; D005782:Gels; D020784:Matrix Metalloproteinase 8; D004318:Doxycycline