title: A high-resolution comparative radiation hybrid map of equine chromosome 4q12-q22.
abstract: In this study, we present a comprehensive 5000-rad radiation hybrid map of a 40-cM region on equine chromosome 4 (ECA4) that contains quantitative trait loci for equine osteochondrosis. We mapped 29 gene-associated sequence tagged site markers using primers designed from equine expressed sequence tags or BAC clones in the ECA4q12-q22 region. Three blocks of conserved synteny, showing two chromosomal breakpoints, were identified in the segment of ECA4q12-q22. Markers from other segments of HSA7q mapped to ECA13p and ECA4p, and a region of HSA7p was homologous to ECA13p. Therefore, we have improved the resolution of the human-equine comparative map, which allows the identification of candidate genes underlying traits of interest.
authors.affiliation: Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17p, 30559 Hannover, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Dierks
authors.forename: S
authors.initials: S
authors.lastname: Mömke
authors.forename: C
authors.initials: C
authors.lastname: Drögemüller
authors.forename: T
authors.initials: T
authors.lastname: Leeb
authors.forename: B P
authors.initials: BP
authors.lastname: Chowdhary
authors.forename: O
authors.initials: O
authors.lastname: Distl
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D033481:Chromosomes, Mammalian; D020224:Expressed Sequence Tags; D006734:Horse Diseases / Q000235:genetics; D006736:Horses / Q000235:genetics*; D006801:Humans; D018895:Microsatellite Repeats; D010007:Osteochondritis / Q000235:genetics / Q000662:veterinary; D040641:Quantitative Trait Loci; D021101:Radiation Hybrid Mapping; D026801:Synteny