Effects of intracerebroventricular injections of free fatty acids, lysophospholipids, or platelet activating factor in a mouse model of orofacial pain.
The present study was carried out to determine the effects of central nervous free fatty acids, lysophospholipids, or platelet activating factor (PAF), in a mouse facial carrageenan injection model of orofacial pain. Mice that received intracerebroventricular (I.C.V.) injection of arachidonic acid or oleic acid showed significantly reduced allodynia and behavioral responses to von Frey hair stimulation of a carrageenan-injected area of the face, at 8 h post-injection, compared to controls that received I.C.V. injection of vehicle. In contrast to free fatty acids, increased responses were observed in mice at 72 h after I.C.V. lysophosphatidic acid or lysophosphatidylcholine injection, and at 8 and 24 h after PAF injection, compared vehicle injected controls. Information regarding pro-nociceptive effect of specific brain lipids may be a useful basis for further studies to explore mechanism.
Vahidy
Wajiha H
WH
Department of Oral and Maxillofacial Surgery, National University of Singapore, Singapore.
Ong
Wei-Yi
WY
Farooqui
Akhlaq A
AA
Yeo
Jin-Fei
JF
D000818:Animals; D002351:Carrageenan; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D005157:Facial Pain / Q000139:chemically induced / Q000188:drug therapy*; D005230:Fatty Acids, Nonesterified / Q000008:administration & dosage*; D007276:Injections, Intraventricular / Q000379:methods; D008246:Lysophospholipids / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010147:Pain Measurement; D010972:Platelet Activating Factor / Q000008:administration & dosage*; D013997:Time Factors
D005230:Fatty Acids, Nonesterified; D008246:Lysophospholipids; D010972:Platelet Activating Factor; D002351:Carrageenan