The correlation of reproduction-related gene expression with germ cell number in DM and PLL gilts.
In this study, the ovarian germ cell number was counted in 3-week-old Duroc x Meishan (DM, n=30) and PIC x (Landrace x Large White) (PLL, n=53) gilts, and the mRNA expression levels of four reproduction-related genes were investigated by quantitative RT-PCR. Correlation of germ cell number with the expression level of these genes was analyzed. Results showed that the germ cell number of DM was significantly higher than that of PLL gilts (P<0.01), although there was no significant difference between the ovarian weight of DM and PLL gilts (P=0.269). No significant correlation existed between germ cell number and ovarian weight in the two gilt groups (R=0.335, P=0.07; R=0.119, P=0.398, respectively). A significant correlation was found between the germ cell number and expression level of ESR and IGF1R mRNA in DM gilts (R=0.648, P<0.05; R=0.757, P<0.01, respectively), but the correlation between the germ cell number and expression level of FSHR and INHBA mRNA did not reach statistical significance. Significant correlation was found between the germ cell number and the expression level of ESR, FSHR, and IGF1R mRNA in PLL gilts (R=0.435, P<0.01; R=0.438, P<0.01; R=0.292, P<0.05, respectively), but not with INHBA mRNA in PLL gilts.
Hu
Yan-Biao
YB
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Pan
Zeng-Xiang
ZX
Xu
Dan
D
Xu
Yin-Xue
YX
Liu
Hong-Lin
HL
Huang
Rui-Hua
RH
Hu
Zhi-Gang
ZG
D000818:Animals; D002452:Cell Count; D005260:Female; D015870:Gene Expression / Q000502:physiology*; D005854:Germ Cells / Q000502:physiology*; D008297:Male; D010053:Ovary / Q000166:cytology*; D012098:Reproduction / Q000235:genetics* / Q000502:physiology; D013223:Statistics as Topic; D013552:Swine