Production kinetics and tensioactive characteristics of biosurfactant from a Pseudomonas aeruginosa mutant grown on waste frying oils.
Various waste frying oils (WFOs) were evaluated as substrates for rhamnolipid production by Pseudomonas aeruginosa mutant EBN-8 in the presence or absence of rhamnolipid precursor, under single-/batch-fed conditions. Soybean WFO was the best substrate, producing 9.3 g rhamnolipid l(-1) with the specific product yield of 2.7 g g(-1) h, under batch-fed cultivation with the addition of rhamnolipid precursor. The surface tension of the cell-free culture broth (CFCB) was 29.1 mN m(-1 )and the interfacial tension against n-hexadecane was <1 mN m(-1). The hydrocarbon/ CFCB systems showed the relative emulsion stability to be in the range of 89.7-92.3.
Raza
Zulfiqar Ali
ZA
Polymer Chemistry Laboratory, National Centre of Excellence in Physical Chemistry, University of Peshawar, Peshawar, 25120, Islamic Republic of Pakistan. zarazapk@yahoo.com
Khan
Muhammad Saleem
MS
Khalid
Zafar M
ZM
Rehman
Asma
A
D006017:Glycolipids / Q000096:biosynthesis*; D009154:Mutation; D009821:Oils / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism*; D014866:Waste Products
D006017:Glycolipids; D009821:Oils; D010938:Plant Oils; D013501:Surface-Active Agents; D014866:Waste Products; C418382:rhamnolipid