title: High-performance liquid chromatography determination of phenyllactic acid in MRS broth.
abstract: Phenyllactic acid (PLA) is an organic acid produced by some strains of lactic acid bacteria (LAB) and concentrations higher than 7.5 mg/ml inhibit growth of moulds and yeasts. Since PLA can be used to select LAB, a rapid, simple and cheap method for its determination is desirable. Typical methods for its analysis in broth are time-consuming, analytically complicated, and have poor recoveries. Herein we propose a simple and rapid method that does not require extraction, but only microfiltration of broth before injection in HPLC. The improved chromatographic conditions allow separation and quantification of PLA with a recovery of 98.7%. The method is highly reproducible with an intraday repeatability of the total peak area of 2.00%, while an interday repeatability of 2.69%.
authors.affiliation: Dipartimento di Protezione e Valorizzazione Agroalimentare, DIPROVAL, Università di Bologna, Via Fratelli Rosselli, 42100 Reggio Emilia, RE, Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Armaforte
authors.forename: Simone
authors.initials: S
authors.lastname: Carri
authors.forename: Giovanni
authors.initials: G
authors.lastname: Ferri
authors.forename: Maria Fiorenza
authors.initials: MF
authors.lastname: Caboni
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017077:Culture Media, Conditioned / Q000032:analysis / Q000737:chemistry; D007773:Lactates / Q000032:analysis*; D007778:Lactobacillus / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D017077:Culture Media, Conditioned; D007773:Lactates; C017648:3-phenyllactic acid