title: Peridinin-chlorophyll-protein reconstituted with chlorophyll mixtures: preparation, bulk and single molecule spectroscopy.
abstract: Reconstitution of the 16 kDa N-terminal domain of the peridinin-chlorophyll-protein, N-PCP, with mixtures of chlorophyll a (Chl a) and Chl b, resulted in 32 kDa complexes containing two pigment clusters, each bound to one N-PCP. Besides homo-chlorophyllous complexes, hetero-chlorophyllous ones were obtained that contain Chl a in one pigment cluster, and Chl b in the other. Binding of Chl b is stronger than that of the native pigment, Chl a. Energy transfer from Chl b to Chl a is efficient, but there are only weak interactions between the two pigments. Individual homo- and hetero-chlorophyllous complexes were investigated by single molecule spectroscopy using excitation into the peridinin absorption band and scanning of the Chl fluorescence, the latter show frequently well resolved emissions of the two pigments.
authors.affiliation: Department Biologie I, Bereich Botanik, Ludwig-Maximilians-Universität München, Menzinger Str. 67, D-80638 München, Germany.
authors.forename: T H P
authors.initials: TH
authors.lastname: Brotosudarmo
authors.forename: E
authors.initials: E
authors.lastname: Hofmann
authors.forename: R G
authors.initials: RG
authors.lastname: Hiller
authors.forename: S
authors.initials: S
authors.lastname: Wörmke
authors.forename: S
authors.initials: S
authors.lastname: Mackowski
authors.forename: A
authors.initials: A
authors.lastname: Zumbusch
authors.forename: C
authors.initials: C
authors.lastname: Bräuchle
authors.forename: H
authors.initials: H
authors.lastname: Scheer
mesh_terms: D000818:Animals; D002338:Carotenoids / Q000737:chemistry* / Q000378:metabolism; D002734:Chlorophyll / Q000737:chemistry* / Q000378:metabolism; D000077194:Chlorophyll A; D056890:Eukaryota / Q000737:chemistry* / Q000378:metabolism; D015800:Protozoan Proteins / Q000737:chemistry* / Q000378:metabolism; D013053:Spectrophotometry / Q000379:methods
chemical_list: D015800:Protozoan Proteins; C073227:peridinin chlorophyll-a protein, Dinophyceae; D002734:Chlorophyll; D002338:Carotenoids; C037184:chlorophyll b; D000077194:Chlorophyll A