title: Advances in the analysis of conformational transitions in peptides using differential scanning calorimetry.
abstract: Differential scanning calorimetry can measure the heat capacity of a protein/peptide solution over a range of temperatures at constant pressure, which is used to determine the enthalpy function of the system. There are several experimental factors that can have a significant impact on the determined enthalpy and subsequent derived thermodynamic parameters. These factors are discussed in terms of sample and instrument preparation, as well as data collection and analysis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Penn State College of Medicine, Hershey, PA, USA.
authors.forename: Werner W
authors.initials: WW
authors.lastname: Streicher
authors.forename: George I
authors.initials: GI
authors.lastname: Makhatadze
mesh_terms: D002152:Calorimetry, Differential Scanning / Q000379:methods*; D006358:Hot Temperature; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D010455:Peptides