title: Multiplex measurement of seven tumor markers using an electrochemical protein chip.
abstract: An electrochemical immunosensor for performing multianalyte measurements of tumor markers is described. The sensor consisted of an array of immunosensing electrodes fabricated on a glass substrate. Each electrode contained a different immobilized antigen and was capable of measuring a specific tumor marker using electrochemical enzyme-based competitive immunoassay. Using this arrangement, multiple analytes could be measured simultaneously by performing the technical operations for a single assay. The biosensor was used to measure the concentrations of seven important tumor markers: AFP, ferritin, CEA, hCG-beta, CA 15-3, CA 125, and CA 19-9. The sensor had excellent precision and accuracy and was comparable in performance to single-analyte ELISAs (1.9-8.1% interassay CV; <2 ng/mL (or units/mL) detection limit for most analytes). Multianalyte assays provide significant advantages over single-analyte tests in terms of cost per test, labor, test throughput, and convenience. We anticipate that chip-based sensors, as described herein, will be suitable for the mass production of economical, miniaturized lab-on-a-chip devices that will have applications in a wide range of clinical, environmental, and biodefense applications.
authors.affiliation: EIC Laboratories, Inc., 111 Downey Street, Norwood, Massachusetts 02062, USA. mwilson@eiclabs.com
authors.forename: Michael S
authors.initials: MS
authors.lastname: Wilson
authors.forename: Weiyan
authors.initials: W
authors.lastname: Nie
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D006063:Chorionic Gonadotropin / Q000032:analysis; D004566:Electrodes; D005293:Ferritins / Q000032:analysis; D011199:Potentiometry / Q000379:methods*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D006063:Chorionic Gonadotropin; D005293:Ferritins