Pre-fractionation of archival frozen tumours for proteomics applications.
We report a protocol for pre-fractionation of proteins from frozen tumours. Using this protocol two separate protein fractions are extracted: Fraction 1 is enriched for cytosolic and Fraction 2 for nuclear/nuclear membrane proteins. The accuracy and reproducibility of the protocol were demonstrated by Western blot analyses and SELDI-TOF-MS profilings. The resulting protein profiles were similar within the respective groups of Fractions 1 and 2 samples, without differences between freshly prepared tumours or those obtained as frozen tissue from biobanks.
Forsberg
Lars
L
Department of Molecular Medicine and Surgery, Section of Medical Genetics, Karolinska Institutet, Karolinska University Hospital-Solna, CMM L8:01, SE-171 76 Stockholm, Sweden.
Larsson
Catharina
C
Sofiadis
Anastasios
A
Lewensohn
Rolf
R
Höög
Anders
A
Lehtiö
Janne
J
D005591:Chemical Fractionation; D015925:Cryopreservation; D006801:Humans; D009369:Neoplasms / Q000737:chemistry* / Q000473:pathology*; D040901:Proteomics / Q000379:methods*