pH-dependent activation of the BarA-UvrY two-component system in Escherichia coli.
The barA and uvrY genes of Escherichia coli encode a two-component sensor kinase and a response regulator, respectively. Although this system plays a major role in the regulation of central carbon metabolism, motility, and biofilm formation by controlling the expression of the CsrB and CsrC noncoding RNAs, the environmental conditions and the physiological signal(s) to which it responds remain obscure. In this study, we explored the effect of external pH on the activity of BarA/UvrY. Our results indicate that a pH lower than 5.5 provides an environment that does not allow activation of the BarA/UvrY signaling pathway.
Mondragón
Verónica
V
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 México D.F., México.
Franco
Bernardo
B
Jonas
Kristina
K
Suzuki
Kazushi
K
Romeo
Tony
T
Melefors
Ojar
O
Georgellis
Dimitris
D
D004926:Escherichia coli / Q000254:growth & development / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation
D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D014157:Transcription Factors; C419603:UvrY protein, E coli; C074276:barA protein, E coli; D010770:Phosphotransferases