Endometrial damage and apoptosis in rats induced by dichlorvos and ameliorating effect of antioxidant vitamins E and C.
We aimed to investigate the effect of subchronic administration of dichlorvos (DDVP) on endometrium and to evaluate ameliorating effects of a combination of Vitamins E and C against DDVP toxicity in the rat. Three groups of rats were used in the experiment. The first group was treated with 4 mg/kg DDVP; the second group was treated with 4 mg/kg body weight DDVP plus Vitamins E and C (DDVP+Vit); the third group was given only corn oil (control). DDVP and DDVP+Vit groups were given DDVP by gavage 5 days a week for 4 weeks at a dose level of 4 mg/kg day by using corn oil as the vechicle. Vitamins E and C were injected at doses of 50 mg/kg i.m. and 20 mg/kg body weight i.p. Histopathological and immunohistochemical examinations for caspase-3 and caspase-9 were accomplished in the endometrium. The level of malondialdehyde (MDA) increased significantly in the DDVP group compared with the control group (p<0.05). MDA significantly decreased in the DDVP+Vit group compared with the DDVP group (p<0.05). Administration of Vitamins E and C along with DDVP significantly reduced the histopathological changes and the extent of apoptosis. In conclusion, subchronic DDVP administration caused endometrial damage and that treatment with a combination of Vitamins E and C reduced endometrial damage caused by DDVP.
Oral
Baha
B
Department of Obstetrics and Gynecology, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey.
Guney
Mehmet
M
Demirin
Hilmi
H
Ozguner
Meltem
M
Giray
Seren Gülsen
SG
Take
Gulnur
G
Mungan
Tamer
T
Altuntas
Irfan
I
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D053148:Caspase 3 / Q000097:blood; D053453:Caspase 9 / Q000097:blood; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage / Q000633:toxicity; D002802:Cholinesterases / Q000097:blood; D004006:Dichlorvos / Q000008:administration & dosage / Q000633:toxicity*; D004031:Diestrus / Q000187:drug effects; D004717:Endometrium / Q000187:drug effects* / Q000473:pathology; D004971:Estrus / Q000187:drug effects; D005207:Fasciculation / Q000139:chemically induced; D005260:Female; D007150:Immunohistochemistry; D007273:Injections, Intramuscular; D007441:Intubation, Gastrointestinal; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000097:blood; D009943:Organophosphorus Compounds / Q000008:administration & dosage / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*; D015430:Weight Gain / Q000187:drug effects
D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D009943:Organophosphorus Compounds; D014810:Vitamin E; D008315:Malondialdehyde; D004006:Dichlorvos; D002802:Cholinesterases; D053148:Caspase 3; D053453:Caspase 9; D001205:Ascorbic Acid