Caffeic acid protects hydrogen peroxide induced cell damage in WI-38 human lung fibroblast cells.
Cytoprotective effect of caffeic acid (3,4-dihydroxy cinnamic acid) on human lung fibroblast (WI-38) cells against hydrogen peroxide induced damage was investigated. Caffeic acid was found to scavenge intracellular reactive oxygen species, and 1,1-diphenyl-2-picrylhydrazyl radical, and thus prevented lipid peroxidation. The caffeic acid protected cell damage of WI-38 cells exposed to hydrogen peroxide (H(2)O(2)), via the activation of extracellular signal regulated kinase protein. Caffeic acid increased the activity of catalase and its protein expression. Hence, from the present study, it is suggestive that caffeic acid protects WI-38 cells against H2O2 damage by enhancing the cellular antioxidant activity.
Kang
Kyoung Ah
KA
Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju-si, Korea.
Lee
Kyoung Hwa
KH
Zhang
Rui
R
Piao
Meijing
M
Chae
Sungwook
S
Kim
Kil Nam
KN
Jeon
You Jin
YJ
Park
Doek Bae
DB
You
Ho Jin
HJ
Kim
Jin Sook
JS
Hyun
Jin Won
JW
D002109:Caffeic Acids / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D019610:Cytoprotection; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000187:drug effects
D002109:Caffeic Acids; D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide; D002374:Catalase; D048049:Extracellular Signal-Regulated MAP Kinases; C040048:caffeic acid