Phases of myogenic cell activation and possible role of dermomyotome cells in teleost muscle formation.
Present knowledge indicates that fibre recruitment (hyperplasia) in developing teleost fish occurs in three distinct phases. However, the origin and relationship of the myogenic precursors activated during the different phases remains unclear. Here, we address this issue using molecular techniques on embryos and larvae of pearlfish, a large cyprinid species. Results provide comprehensive molecular characterisation of cell recruitment over the three phases of myogenesis, identifying muscle types as they arise. Specifically, we show that the myogenic cells arising during 2nd phase myogenesis are clearly different from the myogenic cells arising during the 3rd phase and that the dermomyotome is a major source of myogenic cells driving 2nd phase hyperplasia. These findings are discussed in relation to their implications for the generality of vertebrate developmental patterns.
Steinbacher
P
P
Division of Zoology and Functional Anatomy, Department of Organismic Biology, University of Salzburg, Salzburg, Austria. peter.steinbacher@sbg.ac.at
Haslett
J R
JR
Six
M
M
Gollmann
H P
HP
Sänger
A M
AM
Stoiber
W
W
D000818:Animals; D049109:Cell Proliferation; D003530:Cyprinidae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015870:Gene Expression; D024510:Muscle Development / Q000235:genetics; D017570:MyoD Protein / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D032448:Myoblasts, Skeletal / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D018006:Myogenic Regulatory Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051763:PAX7 Transcription Factor / Q000032:analysis / Q000378:metabolism
D017570:MyoD Protein; D018006:Myogenic Regulatory Factors; D051763:PAX7 Transcription Factor; D018995:Myosin Heavy Chains