RGD peptide-conjugated poly(dimethylsiloxane) promotes adhesion, proliferation, and collagen secretion of human fibroblasts.
A novel technique for conjugating Arg-Gly-Asp (RGD) peptides to poly(dimethylsiloxane) (PDMS) surfaces as well as its application to cell culture is presented in this paper. This technique performs RGD conjugation to PDMS through photochemical immobilization of functional NHS groups to PDMS surface followed with linking RGD peptide to the surface via coupling reaction with NHS. A bifunctional photolinker, N-sulfosuccinimidyl-6-(4'-azido-2'-nitrophenylamino)hexanoate (sulfo-SANPAH), was used to conjugate RGD peptide to the surface. Compared to existing methods for peptide conjugation to PDMS, this technique is convenient, efficient, and free of organic contamination to PDMS surfaces. It can also be used to conjugate other peptides or proteins to most polymeric materials. In addition, cell culture studies showed that the RGD-conjugated PDMS surfaces promoted the adhesion, proliferation, and collagen production of human skin fibroblasts (HSFs). Finally, the RGD-conjugated PDMS surfaces are resistant to autoclaving and UV irradiation, which enables them to be repeatedly used in cell culture studies.
Li
Bin
B
MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pennsylvania 15213, USA.
Chen
Jianxin
J
Wang
James H-C
JH
D001672:Biocompatible Materials; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D004129:Dimethylpolysiloxanes; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing / Q000379:methods; D009842:Oligopeptides / Q000737:chemistry; D012828:Silicones
D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D009842:Oligopeptides; D012828:Silicones; C013830:baysilon; C047981:arginyl-glycyl-aspartic acid; D003094:Collagen