High-performance liquid chromatography determination of phenyllactic acid in MRS broth.
Phenyllactic acid (PLA) is an organic acid produced by some strains of lactic acid bacteria (LAB) and concentrations higher than 7.5 mg/ml inhibit growth of moulds and yeasts. Since PLA can be used to select LAB, a rapid, simple and cheap method for its determination is desirable. Typical methods for its analysis in broth are time-consuming, analytically complicated, and have poor recoveries. Herein we propose a simple and rapid method that does not require extraction, but only microfiltration of broth before injection in HPLC. The improved chromatographic conditions allow separation and quantification of PLA with a recovery of 98.7%. The method is highly reproducible with an intraday repeatability of the total peak area of 2.00%, while an interday repeatability of 2.69%.
Armaforte
Emanuele
E
Dipartimento di Protezione e Valorizzazione Agroalimentare, DIPROVAL, Università di Bologna, Via Fratelli Rosselli, 42100 Reggio Emilia, RE, Italy.
Carri
Simone
S
Ferri
Giovanni
G
Caboni
Maria Fiorenza
MF
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017077:Culture Media, Conditioned / Q000032:analysis / Q000737:chemistry; D007773:Lactates / Q000032:analysis*; D007778:Lactobacillus / Q000378:metabolism; D015203:Reproducibility of Results
D017077:Culture Media, Conditioned; D007773:Lactates; C017648:3-phenyllactic acid