title: Proteomic analysis of root meristems and the effects of acetohydroxyacid synthase-inhibiting herbicides in the root of Medicago truncatula.
abstract: Quantitative proteome analyses of meristematic and nonmeristematic tissues from Medicago truncatula primary and lateral roots and meristem tissues from plants treated with acetohydroxyacid synthase-inhibiting herbicides were made. The accumulation of 81 protein spots changed in meristematic and nonmeristematic tissues and 51 protein spots showed significant changes in accumulation in herbicide-treated meristems. Identified proteins indicate two trends, (i) increased accumulation of cell division and redox-mediating proteins in meristems compared to nonmeristematic tissues and (ii) increased accumulation of pathogenesis-related and decreased accumulation of metabolic proteins in herbicide-treated roots.
authors.affiliation: ARC Centre of Excellence for Integrative Legume Research, Genomic Interactions Group, Research School of Biological Sciences, Australian National University, Canberra ACT 2601, Australia.
authors.forename: Peta
authors.initials: P
authors.lastname: Holmes
authors.forename: Ryan
authors.initials: R
authors.lastname: Farquharson
authors.forename: Prudence J
authors.initials: PJ
authors.lastname: Hall
authors.forename: Barry G
authors.initials: BG
authors.lastname: Rolfe
mesh_terms: D000095:Acetolactate Synthase / Q000037:antagonists & inhibitors; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D006540:Herbicides / Q000633:toxicity*; D013058:Mass Spectrometry; D046913:Medicago truncatula / Q000235:genetics*; D018519:Meristem / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods
chemical_list: D006540:Herbicides; D010940:Plant Proteins; D000095:Acetolactate Synthase