title: Two-dimensional gel proteome reference map of blood monocytes.
abstract: BACKGROUND
  Blood monocytes play a central role in regulating host inflammatory processes through chemotaxis, phagocytosis, and cytokine production. However, the molecular details underlying these diverse functions are not completely understood. Understanding the proteomes of blood monocytes will provide new insights into their biological role in health and diseases.
  
  
  RESULTS
  In this study, monocytes were isolated from five healthy donors. Whole monocyte lysates from each donor were then analyzed by 2D gel electrophoresis, and proteins were detected using Sypro Ruby fluorescence and then examined for phosphoproteomes using ProQ phospho-protein fluorescence dye. Between 1525 and 1769 protein spots on each 2D gel were matched, analyzed, and quantified. Abundant protein spots were then subjected to analysis by mass spectrometry. This report describes the protein identities of 231 monocyte protein spots, which represent 164 distinct proteins and their respective isoforms or subunits. Some of these proteins had not been previously characterized at the protein level in monocytes. Among the 231 protein spots, 19 proteins revealed distinct modification by protein phosphorylation.
  
  
  CONCLUSIONS
  The results of this study offer the most detailed monocyte proteomic database to date and provide new perspectives into the study of monocyte biology.
authors.affiliation: Department of Pathology, The Ohio State University, Columbus, OH, USA. ming.jin@osumc.edu
authors.forename: Ming
authors.initials: M
authors.lastname: Jin
authors.forename: Philip T
authors.initials: PT
authors.lastname: Diaz
authors.forename: Tran
authors.initials: T
authors.lastname: Bourgeois
authors.forename: Charis
authors.initials: C
authors.lastname: Eng
authors.forename: Clay B
authors.initials: CB
authors.lastname: Marsh
authors.forename: Haifeng M
authors.initials: HM
authors.lastname: Wu