AopP, a type III effector protein of Aeromonas salmonicida, inhibits the NF-kappaB signalling pathway.
Aeromonas salmonicida subsp. salmonicida contains a functional type III secretion system that is responsible for the secretion of the ADP-ribosylating toxin AexT. In this study, the authors identified AopP as a second effector protein secreted by this system. The aopP gene was detected in both typical and atypical A. salmonicida isolates and was found to be encoded on a small plasmid of approximately 6.4 kb. Sequence analysis indicates that AopP is a member of the YopJ family of effector proteins, a group of proteins that interfere with mitogen-activated protein kinase (MAPK) and/or nuclear factor kappa B (NF-kappaB) signalling pathways. AopP inhibits the NF-kappaB pathway downstream of IkappaB kinase (IKK) activation, while a catalytically inactivated mutant, AopPC177A, does not possess this inhibitory effect. Unlike other effectors of the YopJ family, such as YopJ and VopA, AopP does not inhibit the MAPK signalling pathway.
Fehr
Désirée
D
Institute of Veterinary Bacteriology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
Casanova
Carlo
C
Division of Molecular Pathology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
Liverman
Amy
A
Department of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, USA.
Blazkova
Hana
H
Division of Molecular Pathology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
Orth
Kim
K
Department of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, USA.
Dobbelaere
Dirk
D
Division of Molecular Pathology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
Frey
Joachim
J
Institute of Veterinary Bacteriology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
Burr
Sarah E
SE
Institute of Veterinary Bacteriology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
D048409:Aeromonas salmonicida / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016328:NF-kappa B / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D016328:NF-kappa B