title: Cloning of the novel gene encoding beta-agarase C from a marine bacterium, Vibrio sp. strain PO-303, and characterization of the gene product.
abstract: The beta-agarase C gene (agaC) of a marine bacterium, Vibrio sp. strain PO-303, consisted of 1,437 bp encoding 478 amino acid residues. beta-Agarase C was identified as the first beta-agarase that cannot hydrolyze neoagarooctaose and smaller neoagarooligosaccharides and was assigned to a novel glycoside hydrolase family.
authors.affiliation: Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie 514-8507, Japan.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Dong
authors.forename: Shinnosuke
authors.initials: S
authors.lastname: Hashikawa
authors.forename: Takafumi
authors.initials: T
authors.lastname: Konishi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamaru
authors.forename: Toshiyoshi
authors.initials: T
authors.lastname: Araki
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012623:Seawater / Q000382:microbiology; D013379:Substrate Specificity; D014733:Vibrio / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D009844:Oligosaccharides; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019624:agarase