Direct nitric oxide measurement in human spermatozoa: flow cytometric analysis using the fluorescent probe, diaminofluorescein.
We have demonstrated that intracellular nitric oxide (NO) can be detected and measured directly in human spermatozoa by flow cytometry using the fluorescent probe 4,5-diaminofluorescein-2/diacetate (10 microM). This method can measure both exogenously added as well as endogenously generated intracellular NO.
Lampiao
Fanuel
F
Department of Medical Physiology and Biochemistry, University of Stellenbosch, Tygerberg, South Africa.
Strijdom
Hans
H
du Plessis
Stefan S
SS
D005434:Flow Cytometry / Q000379:methods*; D019793:Fluorescein; D005456:Fluorescent Dyes; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D009569:Nitric Oxide / Q000032:analysis*; D013094:Spermatozoa / Q000737:chemistry*
C414689:4,5-diaminofluorescein diacetate; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009569:Nitric Oxide; D019793:Fluorescein