Hymenobacter rigui sp. nov., isolated from wetland freshwater.
A non-motile, rod-shaped and pinkish-red-pigmented bacterium, designated strain WPCB131(T), was isolated from freshwater of Woopo wetland, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that this isolate represents a novel member of the genus Hymenobacter with sequence similarities of 93.2-95.5 % to known Hymenobacter species. No other taxa in the phylum Bacteroidetes showed more than 90 % sequence similarity to the isolate. Cells were Gram-negative, oxidase-negative and catalase-positive. Optimum growth was observed at pH 6 and 30 degrees C. The major fatty acids were iso-C(15 : 0) (34.8 %), C(16 : 1)omega5c (15.0 %), anteiso-C(17 : 1) B/iso-C(17 : 1) I (14.4 %) and C(16 : 1)omega7c/iso-C(15 : 0) 2-OH (13.8 %). The DNA G+C content of strain WPCB131(T) was 65 mol%. The isolate could be readily distinguished from other Hymenobacter species using a combination of phenotypic characters. On the basis of the polyphasic evidence presented in this study, it is proposed that strain WPCB131(T) (=IMSNU 14116(T)=KCTC 12533(T)=NBRC 101118(T)) represents a novel species, Hymenobacter rigui sp. nov.
Baik
Keun Sik
KS
Department of Biology, College of Natural Sciences, Sunchon National University, Sunchon 540-742, Republic of Korea.
Seong
Chi Nam
CN
Department of Biology, College of Natural Sciences, Sunchon National University, Sunchon 540-742, Republic of Korea.
Moon
Eun Young
EY
School of Biological Sciences and Institute of Microbiology, Seoul National University, NS70, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Park
Yoon-Dong
YD
School of Biological Sciences and Institute of Microbiology, Seoul National University, NS70, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Yi
Hana
H
School of Biological Sciences and Institute of Microbiology, Seoul National University, NS70, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Chun
Jongsik
J
School of Biological Sciences and Institute of Microbiology, Seoul National University, NS70, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
D015373:Bacterial Typing Techniques; D041964:Cytophagaceae / Q000145:classification / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000378:metabolism*; D005618:Fresh Water / Q000382:microbiology*; D007723:Korea; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D014871:Water Microbiology
D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S