Infectious DNA from Autographa californica nuclear polyhedrosis virus.
Cells from the lepidopteran Spodoptera frugiperda can be successfully transfected in culture with the DNA from Autographa californica nuclear polyhedrosis virus (AcNPV). The calcium chloride precipitation procedure has been used in conjunction with dimethyl sulfoxide treatment of the transfected cells. The highest specific infectivity observed was 6.1 x 10(4) PFU/mug of AcNPV DNA. As judged from a comparison of the restriction patterns of viral DNA preparations, the virus produced in transfection experiments was identical to the virions from which the DNA for transfection was prepared. The transfection procedure described will be useful for the genetic analysis of AcNPV DNA.
Carstens
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Institute of Genetics, University of Cologne, Cologne, Germany.
Tjia
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Doerfler
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