title: Accumulation of class switched IgD-IgM- memory B cells in the cerebrospinal fluid during neuroinflammation.
abstract: Inflammatory diseases of the central nervous system (CNS) are characterized by cerebrospinal fluid (CSF) pleocytosis often involving the recruitment of B cells. Little is still known about B cells that are found in the CSF during neuroinflammation. To address the phenotype of these B cells, we studied the distribution of the major B cell subsets in peripheral blood (PB) and CSF of 25 patients with inflammatory diseases of the nervous system by flow cytometry. Six different B cell subsets were identified in PB and CSF according to the surface expression of IgM, IgD, CD27 and CD19. In all patients analysed, memory B cells outnumbered naïve B cells in the CSF, whereas naïve B cells were more prevalent in PB. The accumulation of memory B cells in the CSF was largely due to the recruitment of IgM-IgD- class switched memory B cells. The distribution of IgM+IgD+, IgM-IgD+, IgM+IgD- memory cells and immature cells did not differ significantly between CSF and PB. These findings demonstrate a selective recruitment of IgM-IgD- memory B cells to the CSF suggesting a specific role of these cells during neuroinflammation.
authors.affiliation: Neuroimmunology Group, Department of Neurology, Heinrich Heine-University, Moorenstr. 5, 40225 Düsseldorf, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Cepok
authors.forename: Gloria
authors.initials: G
authors.lastname: von Geldern
authors.forename: Verena
authors.initials: V
authors.lastname: Grummel
authors.forename: Sonja
authors.initials: S
authors.lastname: Hochgesand
authors.forename: Handan
authors.initials: H
authors.lastname: Celik
authors.forename: Hanspeter
authors.initials: H
authors.lastname: Hartung
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hemmer
mesh_terms: D000328:Adult; D000368:Aged; D000917:Antibody Formation / Q000276:immunology; D018941:Antigens, CD19 / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D002555:Cerebrospinal Fluid / Q000166:cytology / Q000276:immunology*; D004660:Encephalitis / Q000134:cerebrospinal fluid / Q000276:immunology* / Q000503:physiopathology; D005260:Female; D006801:Humans; D017578:Immunoglobulin Class Switching / Q000276:immunology; D007072:Immunoglobulin D / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D007156:Immunologic Memory / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology
chemical_list: D018941:Antigens, CD19; D001323:Autoantibodies; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7