title: Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins.
abstract: We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 A) than any previously obtained with biarsenical dyes.
authors.affiliation: Departamento de Química Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CIHIDECAR, CONICET, Ciudad Universitaria-Pabellón II, 1428, Buenos Aires, Argentina.
authors.forename: Carla C
authors.initials: CC
authors.lastname: Spagnuolo
authors.forename: Rudolf J
authors.initials: RJ
authors.lastname: Vermeij
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Jares-Erijman
mesh_terms: D001152:Arsenicals / Q000737:chemistry*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis*; D004355:Drug Stability; D005452:Fluoresceins / Q000737:chemistry*; D005454:Fluorescence Polarization; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009842:Oligopeptides / Q000032:analysis; D010777:Photochemistry
chemical_list: D001152:Arsenicals; D005452:Fluoresceins; D006845:Hydrocarbons, Fluorinated; D009842:Oligopeptides; D003545:Cysteine