title: Survey of extended-spectrum beta-lactamases in Escherichia coli isolates from a Portuguese hospital and characterisation of a novel class 1 integron (In60A) carrying the blaCTX-M-9 gene.
abstract: Between November 2001 and November 2004, 231 Escherichia coli isolates resistant to beta-lactam antibiotics were identified. In 14 isolates, bla(TEM-24) (2 isolates), bla(TEM-52) (5 isolates) and bla(TEM-26) (7 isolates) were identified. In 145 E. coli isolates with the same M13 fingerprinting profile and the same resistance phenotype, the bla(CTX-M-15) gene was found in association with an insertion sequence ISEcp1. The bla(CTX-M-2) gene was identified in one E. coli isolate (290HSM), and in other E. coli isolate (246HSM) the bla(CTX-M-9) gene was contained in a new complex sul1-type class 1 integron (named In60A). This is the first report of three cefotaximases (CTX-M-15, CTX-M-2 and CTX-M-9) in E. coli isolates from a Portuguese hospital.
authors.affiliation: Laboratory of Microbiology, Faculty of Pharmacy, Lisbon, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Brizio
authors.forename: Sergio
authors.initials: S
authors.lastname: Vasco
authors.forename: Ana Rita
authors.initials: AR
authors.lastname: Gonçalves
authors.forename: Luis Manuel
authors.initials: LM
authors.lastname: Lito
authors.forename: Jose Melo
authors.initials: JM
authors.lastname: Cristino
authors.forename: Maria Jose
authors.initials: MJ
authors.lastname: Salgado
authors.forename: Aida
authors.initials: A
authors.lastname: Duarte
mesh_terms: D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006761:Hospitals; D006801:Humans; D032023:Integrons / Q000235:genetics*; D008297:Male; D011174:Portugal / Q000453:epidemiology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C412613:CTX-M-9 protein, E coli; D001618:beta-Lactamases