title: Cloning, expression, purification, crystallization and initial crystallographic analysis of the preprotein translocation ATPase SecA from Thermus thermophilus.
abstract: The Thermus thermophilus gene encoding the preprotein translocation ATPase SecA was cloned and expressed and the purified protein was crystallized by the hanging-drop vapour-diffusion technique in two different space groups P3(1(2))21 (a = b = 168.6, c = 149.8 A) and P6(1(5))22 (a = b = 130.9, c = 564.6 A). The crystals, improved by macroseeding, diffracted to beyond 2.8 and 3.5 A resolution for the trigonal and hexagonal crystal forms, respectively. Structure determination using the multiple isomorphous replacement method is in progress.
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 402B Kaul Genetics Building, 720 20th Street South, Birmingham, AL 35294, USA.
authors.forename: Marina N
authors.initials: MN
authors.lastname: Vassylyeva
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mori
authors.forename: Tomoya
authors.initials: T
authors.lastname: Tsukazaki
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Tahir H
authors.initials: TH
authors.lastname: Tahirov
authors.forename: Koreaki
authors.initials: K
authors.lastname: Ito
authors.forename: Dmitry G
authors.initials: DG
authors.lastname: Vassylyev
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D011498:Protein Precursors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D016963:Thermus thermophilus / Q000201:enzymology* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D011498:Protein Precursors; D000069816:SEC Translocation Channels; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins