title: [Establishment of chemiluminescence immunoassay for detecting Staphylococcal enterotoxin B and C1].
abstract: OBJECTIVE
  To establish chemiluminescence immunoassay (CLIA) for detecting staphylococcal enterotoxin B (SEB) and C1 (SEC1) and compare its sensitivity and stability with ELISA.
  
  
  METHODS
  The anti-SEB and SEC1 monoclonal antibodies (mAb) were purified by Q Sepharose Fast Flow chromatographic column. The alkaline phosphatase (AP) conjugated mAbs FMMU-SEB.D6 and FMMU-SEC1.C4 were used as detecting antibodies and the FMMU-SEB.B4 and FMMU-SEC1.G8 mAbs were used as coating antibodies in both methods. Phenolphthalein monophosphate (PMP) and lumigen APS-5 were employed as substrates for AP in ELISA and CLIA, respectively. The light was detected and measured by the GENios analyzer (TECAN Group Ltd.). The sensitivities and detect ranges of CLIA and ELISA methods were compared.
  
  
  RESULTS
  Compared with ELISA, CLIA was more sensitive (0.1 ng/mL vs 0.39 ng/mL) and timesaving. Furthermore, the liner range of CLIA was broader than that of ELISA (0.78-50 ng/mL vs 3.125-50 ng/mL).
  
  
  CONCLUSIONS
  CLIA for detecting SEB and SEC1 are established successfully which may be useful in food monitoring, epidemiology survey and detecting SE contaminated samples in environment.
authors.affiliation: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Li-jie
authors.initials: LJ
authors.lastname: Chen
authors.forename: Zhen-shi
authors.initials: ZS
authors.lastname: Liu
authors.forename: Bang-quan
authors.initials: BQ
authors.lastname: Dong
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Bo-quan
authors.initials: BQ
authors.lastname: Jin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004768:Enterotoxins / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D012680:Sensitivity and Specificity; D013210:Staphylococcus; D013696:Temperature
chemical_list: D000911:Antibodies, Monoclonal; D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal; C034959:enterotoxin C, staphylococcal