title: Tissue engineering of tooth crown, root, and periodontium.
abstract: Tissue engineering of teeth requires the coordinated formation of correctly shaped crowns, roots, and periodontal ligament. Previous studies have shown that the dental mesenchyme controls crown morphogenesis and epithelial histogenesis during tooth development in vivo, but little is known about the inductive potential of dissociated mesenchymal cells used in ex vivo cultures. A 2-step method is described in which, by using different types of reassociations between epithelial and mesenchymal tissues and/or cells from mouse embryos, reassociations were cultured in vitro before in vivo implantation. In vitro, the reassociated tissues developed and resulted in tooth-like structures that exhibited normal epithelial histogenesis and allowed the functional differentiation of odontoblasts and ameloblasts. After implantation, the reassociations formed roots and periodontal ligament, the latter connected to developing bone. The shape of the crown, initially suspected to depend on the integrity of the mesenchyme, could be modulated by adjusting the number of dissociated mesenchymal cells reassociated with the epithelial compartment. Based on these results, we propose a refined strategy for tooth tissue engineering that may help to eventually generate morphologically defined teeth.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, UMR S595, Faculté de Chirurgie Dentaire, Université Louis Pasteur, Strasbourg, France. Bing.Hu@odonto-ulp.u-strasbg.fr
authors.forename: Bing
authors.initials: B
authors.lastname: Hu
authors.forename: Amal
authors.initials: A
authors.lastname: Nadiri
authors.forename: Sabine
authors.initials: S
authors.lastname: Kuchler-Bopp
authors.forename: Fabienne
authors.initials: F
authors.lastname: Perrin-Schmitt
authors.forename: Heiko
authors.initials: H
authors.lastname: Peters
authors.forename: Hervé
authors.initials: H
authors.lastname: Lesot
mesh_terms: D000818:Animals; D003442:Crowns; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D010519:Periodontium; D046509:Tissue Culture Techniques; D023822:Tissue Engineering; D019228:Tooth Crown; D014092:Tooth Root