CD4+ T and B cells cooperate in the immunoregulation of Experimental Autoimmune Myasthenia Gravis.
C57Bl6 mice (B6 mice) immunized with Torpedo acetylcholine receptor (TAChR) in Freund's adjuvants (FA) develop Experimental Autoimmune Myasthenia Gravis (EAMG). In mouse EAMG Th2 cytokines may be protective. Aluminum hydroxide (Alum) was used to immunize B6 mice to the TAChR and prime CD4+ T and B cells secreting Th2 cytokines. Mice immunized with TAChR/Alum developed anti-AChR CD4+ T cells response, but minimal antibody levels and symptoms. TAChR/Alum treatments prior immunization with TAChR/FA protected mice from EAMG. Cell transfer experiments demonstrated that B and CD4+ T cells mediated the protective effect by causing intense reduction of complement-fixing anti-TAChR IgG subclasses.
Milani
Monica
M
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA. milan006@umn.edu
Ostlie
Norma
N
Wu
Huiyun
H
Wang
Wei
W
Conti-Fine
Bianca M
BM
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000536:Aluminum Hydroxide / Q000276:immunology; D000818:Animals; D001323:Autoantibodies / Q000097:blood; D001324:Autoantigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005620:Freund's Adjuvant / Q000276:immunology; D015870:Gene Expression; D020869:Gene Expression Profiling; D007074:Immunoglobulin G / Q000097:blood; D008211:Lymphocyte Cooperation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis; D011950:Receptors, Cholinergic / Q000276:immunology*; D014101:Torpedo
D000276:Adjuvants, Immunologic; D001323:Autoantibodies; D001324:Autoantigens; D007074:Immunoglobulin G; D011950:Receptors, Cholinergic; D000536:Aluminum Hydroxide; D005620:Freund's Adjuvant