EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. I. Evidence for stage-specific gene expression in aerial conidia, in vitro blastospores and submerged conidia.
The entomopathogenic fungus Beauveria (Cordyceps) bassiana holds much promise as a pest biological control agent. B. bassiana produces at least three in vitro single cell infectious propagules, including aerial conidia, vegetative cells termed blastospores and submerged conidia, that display different morphological, biochemical and virulence properties. Populations of aerial conidia, blastospores and submerged conidia were produced on agar plates, rich liquid broth cultures and under conditions of nutrient limitation in submerged cultures, respectively. cDNA libraries were generated from mRNA isolated from each B. bassiana cell type and approximately 2,500 5' end sequences were determined from each library. Sequences derived from aerial conidia clustered into 284 contigs and 963 singlets, with those derived from blastospores and submerged conidia forming 327 contigs with 788 singlets, and 303 contigs and 1,079 contigs, respectively. Almost half (40-45 %) of the sequences in each library displayed either no significant similarity (e value >10(-4)) or similarity to hypothetical proteins found in the NCBI database. The expressed sequence tag dataset also included sequences representing a significant portion of proteins in cellular metabolism, information storage and processing, transport and cell processes, including cell division and posttranslational modifications. Transcripts encoding a diverse array of pathogenicity-related genes, including proteases, lipases, esterases, phosphatases and enzymes producing toxic secondary metabolites, were also identified. Comparative analysis between the libraries identified 2,416 unique sequences, of which 20-30 % were unique to each library, and only approximately 6 % of the sequences were shared between all three libraries. The unique and divergent representation of the B. bassiana transcriptome in the cDNA libraries from each cell type suggests robust differential gene expression profiles in response to environmental conditions.
Cho
Eun-Min
EM
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA.
Liu
Li
L
Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32611, USA.
Farmerie
William
W
Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32611, USA.
Keyhani
Nemat O
NO
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA.
D000818:Animals; D032661:Cordyceps / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D004271:DNA, Fungal / Q000032:analysis; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D066298:In Vitro Techniques; D007313:Insecta / Q000382:microbiology*; D008018:Life Cycle Stages / Q000502:physiology*; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000502:physiology
D004271:DNA, Fungal