Diagnosis of gastric syphilis by direct immunofluorescence staining and real-time PCR testing.
We report on a case of gastric syphilis in a patient with chronic dyspepsia. The diagnosis was established by serology and the demonstration of spirochetes in diffusely inflammed gastric mucosa by staining with a fluorescent monoclonal antibody specific for pathogenic treponemes and by the detection of specific treponemal DNA sequences by a real-time PCR.
Chen
Cheng-Yen
CY
Division of STD Prevention, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, Mail Stop G-39, 1600 Clifton Road, Atlanta, GA 30333, USA. cyc1@cdc.gov
Chi
Kai-Hua
KH
George
Robert W
RW
Cox
David L
DL
Srivastava
Amitabh
A
Rui Silva
Mário
M
Carneiro
Fátima
F
Lauwers
Gregory Y
GY
Ballard
Ronald C
RC
D000328:Adult; D004269:DNA, Bacterial / Q000032:analysis; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D005753:Gastric Mucosa / Q000382:microbiology; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D013272:Stomach Diseases / Q000175:diagnosis* / Q000382:microbiology*; D013587:Syphilis / Q000175:diagnosis* / Q000382:microbiology; D014210:Treponema pallidum / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial