title: Regulation of IL-27p28 gene by lipopolysaccharide in dendritic DC2.4 cells.
abstract: To elucidate the regulation of IL-27p28 gene, we analyzed the promoter region of the gene in DC2.4 cells with or without lipopolysaccharide (LPS)-treatment. The results indicate that a region (-648 to -364) of p28 promoter was responsible for LPS-induction. EMSA with DNA probes within the region reveals that binding of GATA motif bound proteins was decreased by LPS-treatment. We identified one of the proteins as non-POU domain-containing octamer binding protein (NonO). Taken together, LPS-induced activation of IL-27p28 gene can be accounted for by the displacement of bound NonO protein from the IL-27p28 promoter.
authors.affiliation: Laboratory of Molecular Biology, Medical Research Center, Kochi Medical School, Kochi, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kamakura
authors.forename: Keiko
authors.initials: K
authors.lastname: Morisawa
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Komi
authors.forename: Ayako
authors.initials: A
authors.lastname: Tomatani
authors.forename: Fumiji
authors.initials: F
authors.lastname: Saito
authors.forename: Yuko
authors.initials: Y
authors.lastname: Konishi
authors.forename: Ya
authors.initials: Y
authors.lastname: Jin
authors.forename: Takashi
authors.initials: T
authors.lastname: Manabe
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kuroda
authors.forename: Shosuke
authors.initials: S
authors.lastname: Imai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizuguchi
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D002460:Cell Line; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: C506769:Il27 protein, rat; D007378:Interleukins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins