The HBP1 transcriptional repressor participates in RAS-induced premature senescence.
Oncogene-mediated premature senescence has emerged as a potential tumor-suppressive mechanism in early cancer transitions. Previous work shows that RAS and p38 MAPK participate in premature senescence, but transcriptional effectors have not been identified. Here, we demonstrate that the HBP1 transcriptional repressor participates in RAS- and p38 MAPK-induced premature senescence. In cell lines, we had previously isolated HBP1 as a retinoblastoma (RB) target but have determined that it functions as a proliferation regulator by inhibiting oncogenic pathways as a transcriptional repressor. In primary cells, the results indicate that HBP1 is a necessary component of premature senescence by RAS and p38 MAPK. Similarly, a knockdown of WIP1 (a p38 MAPK phosphatase) induced premature senescence that also required HBP1. Furthermore, HBP1 requires regulation by RB, in which few transcriptional regulators for premature senescence have been shown. Together, the data suggest a model in which RAS and p38 MAPK signaling engage HBP1 and RB to trigger premature senescence. As an initial step toward clinical relevance, a bioinformatics approach shows that the relative expression levels of HBP1 and WIP1 correlated with decreased relapse-free survival in breast cancer patients. Together, these studies highlight p38 MAPK, HBP1, and RB as important components for a premature-senescence pathway with possible clinical relevance to breast cancer.
Zhang
Xiaowei
X
Department of Biochemistry, Tufts University School of Medicine, Boston, MA 02111, USA.
Kim
Jiyoung
J
Ruthazer
Robin
R
McDevitt
Michael A
MA
Wazer
David E
DE
Paulson
K Eric
KE
Yee
Amy S
AS
D001665:Binding Sites; D001943:Breast Neoplasms / Q000175:diagnosis*; D002460:Cell Line; D016922:Cellular Senescence; D004261:DNA Replication; D030541:Databases, Genetic; D006609:High Mobility Group Proteins / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D015689:Oncogene Protein p21(ras) / Q000502:physiology*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D000071636:Protein Phosphatase 2C; D012097:Repressor Proteins / Q000502:physiology*; D016160:Retinoblastoma Protein / Q000378:metabolism / Q000502:physiology; D015398:Signal Transduction; D014162:Transfection; D048051:p38 Mitogen-Activated Protein Kinases / Q000502:physiology*
C486328:HBP1 protein, human; D006609:High Mobility Group Proteins; D012097:Repressor Proteins; D016160:Retinoblastoma Protein; D048051:p38 Mitogen-Activated Protein Kinases; C000607458:PPM1D protein, human; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C; D015689:Oncogene Protein p21(ras)