title: Antioxidative and in vitro hepatoprotective activity of Bupleurum kaoi leaf infusion.
abstract: The roots of Bupleurus spp. have been used in traditional Chinese herbal medicine for curing liver diseases. Although bioactive saikosaponins have been detected in the leaves as well as in the roots, the aerial parts of the plants are discarded as waste. In the present study, a leaf infusion of B. kaoi Liu, Chao et Chuang, an indigenous Bupleurus species in Taiwan, was prepared and the antioxidant properties and in vitro hepatoprotective activity were demonstrated. The results show that the leaf infusion exerted DPPH free radical scavenging activity, inhibitory capacity on superoxide anion formation and superoxide anion scavenging activity. The hepatotoxicity of acetaminophen (APAP) and carbon tetrachloride (CCl4) on the rat liver cells were also decreased by the leaf infusion.
authors.affiliation: Institute of Food Science and Technology, National Taiwan University, No.59, Lane 144, Sec. 4, Keelung Rd., Da-an District, Taipei City 106, Taiwan, ROC.
authors.forename: Chu-Ting
authors.initials: CT
authors.lastname: Liu
authors.forename: Pei-Ting
authors.initials: PT
authors.lastname: Chuang
authors.forename: Ching-Yi
authors.initials: CY
authors.lastname: Wu
authors.forename: Yih-Ming
authors.initials: YM
authors.lastname: Weng
authors.forename: Wenlung
authors.initials: W
authors.lastname: Chen
authors.forename: Chin-Yin
authors.initials: CY
authors.lastname: Tseng
mesh_terms: D000082:Acetaminophen / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D029972:Bupleurum / Q000737:chemistry*; D002251:Carbon Tetrachloride / Q000633:toxicity; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000517:prevention & control; D022781:Hepatocytes / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects*; D008297:Male; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D013481:Superoxides; D000082:Acetaminophen; D002251:Carbon Tetrachloride; C004931:1,1-diphenyl-2-picrylhydrazyl