title: Regulation of MAL1+ gene expression encoding maltase in Schizosaccharomyces pombe by added inositol.
abstract: In this study, the effects of inositol addition on maltase activity and expression of MAL1+ gene encoding maltase in Schizosaccharomyces pombe were investigated. The maximum specific maltase activity was observed, when the concentration of inositol reached 6.0 microg/ml in the synthetic medium containing 2.0% glucose. At 1.0 microg/ml inositol concentration, the maltase activity continuously decreased, as initial glucose concentration was higher than 0.1%. mRNA encoding maltase and phosphatidylinositol (PI) content were higher in the cells grown in the synthetic medium with 6.0 microg/ml of inositol and 2.0% glucose than those with 1.0 microg/ml of inositol. These results demonstrated that higher inositol concentration in the synthetic medium could derepress MAL1+ gene expression in S. pombe and PI might be involved in derepression of MAL1+ gene expression in S. pombe probably by PI-type signalling pathway.
authors.affiliation: UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road No.5, Qingdao, 266003, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Yao
authors.forename: Zhenming
authors.initials: Z
authors.lastname: Chi
authors.forename: Susu
authors.initials: S
authors.lastname: He
mesh_terms: D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal; D005947:Glucose / Q000494:pharmacology; D007294:Inositol / Q000494:pharmacology; D010743:Phospholipids / Q000096:biosynthesis; D012568:Schizosaccharomyces / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D000520:alpha-Glucosidases / Q000235:genetics* / Q000378:metabolism
chemical_list: D010743:Phospholipids; D007294:Inositol; D000520:alpha-Glucosidases; D005947:Glucose