Mass spectrometry identification of circulating alpha-1-B glycoprotein, increased in aged female C57BL/6 mice.
In this study, we surveyed the profiles of mouse circulating proteins by 2-dimensional SDS-PAGE in different strains, sexes and ages. Among visible protein spots on 2-D gels with silver-staining, we identified a unique set of 7 seemingly-related proteins whose levels were consistently elevated in older C57BL/6 female mice. This set of 7 proteins was absent in C57BL/6 males or in BALB/c mice of either sex of any age. When C57BL/6 female mice were crossed with BALB/c males, the age-related increase of these proteins became sporadic and not linear in the F1 offspring. All 7 spots of this protein group were picked and subjected to identification by mass spectrometric analysis after tryptic digestion. The results showed that all 7 spots were different isoforms of alpha(1)B-glycoprotein with different degrees of post-translational modifications, such as phosphorylation. These results suggest that alpha(1)B-glycoprotein changes in mice in a sex and age dependent manner.
Stehle
John R
JR
Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
Weeks
Mark E
ME
Lin
Kai
K
Willingham
Mark C
MC
Hicks
Amy M
AM
Timms
John F
JF
Cui
Zheng
Z
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009961:Orosomucoid / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009961:Orosomucoid