title: Gene expression profiling of human synovial sarcoma cell line (Hs701.T) in response to IL-1beta stimulation.
abstract: OBJECTIVE
  Synovial sarcoma (SS) is a malignant mesenchymal tumor that accounts for 5-10% of all soft tissue sarcoma. IL-1beta, a pleiotrophic cytokine, has been found in the tumor microenvironment which plays crucial roles in the pathogenesis of tumors.
  
  
  METHODS
  In this study, we used Hs701.T as a cellular model to study the short-term (4-h) and long-term (48-h) stimulatory effect of IL-1beta on cell proliferation and differential gene expression.
  
  
  RESULTS
  The results showed that IL-1beta can stimulate cell proliferation through activation of NF-kappaB and AP-1 transcription factors; sequentially triggers the expression of genes related to tumor progression. The microarray data indicated that most of the up-regulated genes were related to tumor progression. Five candidate genes which are involved in the mediation of proliferation (IL-6), apoptosis (Hsp27 and Daxx), and angiogenesis (PlGF and SPARC) were further validated by RT-PCR.
  
  
  CONCLUSIONS
  These findings may be useful for understanding the pathogenesis of synovial sarcoma.
authors.affiliation: Hung Lai Ching Laboratory of Biomedical Science, Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong.
authors.forename: W Y
authors.initials: WY
authors.lastname: Ha
authors.forename: X J
authors.initials: XJ
authors.lastname: Li
authors.forename: P Y K
authors.initials: PY
authors.lastname: Yue
authors.forename: D Y L
authors.initials: DY
authors.lastname: Wong
authors.forename: K K M
authors.initials: KK
authors.lastname: Yue
authors.forename: W S
authors.initials: WS
authors.lastname: Chung
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.forename: P Y
authors.initials: PY
authors.lastname: Leung
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: R N S
authors.initials: RN
authors.lastname: Wong
mesh_terms: D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D018450:Disease Progression; D005347:Fibroblasts / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism*; D009369:Neoplasms / Q000098:blood supply; D009389:Neovascularization, Pathologic; D020411:Oligonucleotide Array Sequence Analysis; D013584:Sarcoma, Synovial / Q000378:metabolism*; D013583:Synovial Membrane / Q000473:pathology*; D013997:Time Factors
chemical_list: D053583:Interleukin-1beta