Flow cytometry and immunomorphological characteristics of apoptosis in hepatocytes of white mice during aging.
Apoptosis, sometimes called "programmed cell death", the process that goes on continuously throughout life has received phenomenal attention in the past few years. In the process of aging of organism, most of organs undergo morphological and functional changes at various frequencies. Initially, the role of apoptosis regarding aging was evaluated negatively, however, at present the issue is in the process of reconsideration. The experiments were performed on 74 white mice, distributed in three age groups (juveniles, adults, and senescents). Apoptotic nuclei were detected by immunomorphological and flow cytometry assay. So, the analysis of the data obtained that apoptosis in hepatocytes of white mice decreases with age and afterwards increases in a credible way. The maximum value is reached in the senescent mice. It has been considered, that aging increases the susceptibility of hepatocytes to apoptosis in white mice.
Gujabidze
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N
Department of Histology, Cytology and Embryology, Tbilisi State Medical University.
Rukhadze
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R
D000818:Animals; D017209:Apoptosis; D002467:Cell Nucleus / Q000648:ultrastructure; D016922:Cellular Senescence; D005434:Flow Cytometry; D022781:Hepatocytes / Q000648:ultrastructure*; D051379:Mice; D008815:Mice, Inbred Strains