title: No differences in the DNA methylation pattern in mouse zygotes produced in vivo, in vitro, or by intracytoplasmic sperm injection.
abstract: The pattern and degree of pronuclear DNA methylation has been evaluated in naturally, in in vitro, or in intracytoplasmic sperm injection (ICSI)-produced mouse embryos. The results show no differences between all experimental groups and confirm the relative safety of IVF and ICSI.
authors.affiliation: Department of Cell Ultrastructure and Molecular Biology, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Fulka
authors.forename: Josef
authors.initials: J
authors.lastname: Fulka
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics*; D019175:DNA Methylation; D044127:Epigenesis, Genetic / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR; D020554:Sperm Injections, Intracytoplasmic; D015053:Zygote / Q000166:cytology* / Q000502:physiology*
chemical_list: D004247:DNA