Identification of human alpha-synuclein specific single chain antibodies.
Parkinson's disease (PD) is a common neurodegenerative disease of unknown etiology. Evidence suggests a role for protein misfolding in disease pathogenesis. One pathologic feature observed in dopaminergic neurons is the intracytoplasmic eosinophilic inclusions known as Lewy bodies. One component of Lewy bodies, the presynaptic protein, alpha-synuclein forms oligomers and higher order aggregates and is proposed to be involved in dopaminergic neuronal death. In an effort to discriminate between alpha-synuclein conformational forms as well as design potential disruptors of pathogenic misfolding we panned a human phage antibody library for anti-synuclein single chain antibodies (scFvs). We identified six scFvs which recognize different conformers of alpha-synuclein in both an ELISA and Western blot analysis. These scFvs may further our understanding of alpha-synuclein's role in PD.
Maguire-Zeiss
Kathleen A
KA
Department of Neurology and the Center for Aging and Developmental Biology, University of Rochester School of Medicine and Dentistry, NY 14642, USA.
Wang
Charlotte I
CI
Yehling
Eric
E
Sullivan
Mark A
MA
Short
Douglas W
DW
Su
Xiaomin
X
Gouzer
Geraldine
G
Henricksen
Leigh A
LA
Wuertzer
Charles A
CA
Federoff
Howard J
HJ
D006801:Humans; D007135:Immunoglobulin Variable Region / Q000032:analysis / Q000737:chemistry* / Q000276:immunology*; D051844:alpha-Synuclein / Q000032:analysis / Q000737:chemistry* / Q000276:immunology*
D007135:Immunoglobulin Variable Region; D051844:alpha-Synuclein