title: Proliferative arrest and activation of apoptosis related genes in Rolly Protein-silenced cells.
abstract: Here we describe a novel small polypeptide expressed in chick embryo and mouse adult tissues referred to as Rolly Protein (Rolp), expressed at the highest levels in tibial cartilage and lung respectively. Investigating its putative role in cartilage differentiation we found that its expression is restricted to proliferative stages consistently with a decreased proliferation rate observed in Rolp-silenced cells. Additional functional studies demonstrate that inhibition of Rolp expression causes a transcription modulation of genes involved in apoptosis. The results here provided strongly suggest an active role of Rolp in the control of cell proliferation and apoptosis.
authors.affiliation: Dipartimento di Oncologia Biologia e Genetica, Università di Genova, Italy. aldo.pagano@istge.it
authors.forename: Aldo
authors.initials: A
authors.lastname: Pagano
authors.forename: Laura
authors.initials: L
authors.lastname: Tonachini
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Monticone
authors.forename: Federico
authors.initials: F
authors.lastname: Tortelli
authors.forename: Manuele
authors.initials: M
authors.lastname: Castelnuovo
authors.forename: Nadia
authors.initials: N
authors.lastname: Randazzo
authors.forename: Sara
authors.initials: S
authors.lastname: Tavella
authors.forename: Eddi
authors.initials: E
authors.lastname: Di Marco
authors.forename: Ranieri
authors.initials: R
authors.lastname: Cancedda
authors.forename: Patrizio
authors.initials: P
authors.lastname: Castagnola
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D049109:Cell Proliferation; D002478:Cells, Cultured; D002642:Chick Embryo; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D020219:Chondrogenesis / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D020868:Gene Silencing; D016350:Leucine Zippers / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D051017:Apoptosis Regulatory Proteins; D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; C000589631:Rolly protein, mouse