title: Activated human Hageman factor (XII).
abstract: An improved method for the isolation of highly purified active human Hageman factor has been described. An overall recovery of 25% with a purification of 10(6) has been achieved. Certain of the physical and chemical characteristics of XII have been elucidated. Selective chemical modification of functional groups and a variety of enzymatic assays have been employed in an effort to shed light on the mechanism of action of this procoagulant.
authors.affiliation: J. K. and Susie L. Wadley Research Institute, Dallas, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Speer
authors.forename: H
authors.initials: H
authors.lastname: Ridgway
authors.forename: J M
authors.initials: JM
authors.lastname: Hill
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005174:Factor XII / Q000737:chemistry / Q000302:isolation & purification*; D015956:Factor XIIa / Q000737:chemistry / Q000302:isolation & purification*; D005612:Freeze Drying; D006801:Humans; D007525:Isoelectric Focusing
chemical_list: D000596:Amino Acids; D005174:Factor XII; D015956:Factor XIIa