Preparation, characterization, photocytotoxicity assay of PLGA nanoparticles containing zinc (II) phthalocyanine for photodynamic therapy use.
Nanoparticles containing Zinc (II) Phthalocyanine (ZnPc) were prepared by a spontaneous emulsification diffusion method utilizing poly-(D,L lactic-co-glycolic acid) (PLGA), characterized and available in cellular culture. The process yield and encapsulation efficiency were 60% and 80%, respectively. The nanoparticles have a mean diameter of 200 nm, a narrow size distribution with polydispersive index of 0.15, smooth surface and spherical shape. ZnPc loaded nanoparticles maintain their photophysical behaviour after the encapsulation process. Photosensitizer released from nanoparticles was sustained with a burst effect of 10% for 3 days. The photocytotoxicity was evaluated on P388-D1 cells. They were incubated with ZnPc loaded Np by 6 h and exposed to light (675 nm) for 120 s, and light dose of 30 J cm-2. After 24 h of incubation, the cellular viability was determined, obtaining 60% of cellular death. All the physical-chemical and photobiological measurements performed allowed one conclude that ZnPc loaded PLGA nanoparticles are a promising drug delivery system for PDT.
Ricci-Júnior
Eduardo
E
Faculty of Pharmaceutical Sciences, Laboratory of Pharmaceutical Technology, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Marchetti
Juliana M
JM
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004339:Drug Compounding / Q000379:methods; D005453:Fluorescence; D007211:Indoles / Q000008:administration & dosage* / Q000494:pharmacology; D054833:Isoindoles; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D008808:Mice, Inbred CBA; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D009942:Organometallic Compounds / Q000008:administration & dosage* / Q000494:pharmacology; D010316:Particle Size; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000494:pharmacology; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011827:Radiation; D013499:Surface Properties; D017967:Zinc Compounds
D001672:Biocompatible Materials; D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D011108:Polymers; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid