title: A1 adenosine receptors in microglia control glioblastoma-host interaction.
abstract: We report that experimental glioblastoma grow more vigorously in A(1) adenosine receptor (A(1)AR)-deficient mice associated with a strong accumulation of microglial cells at and around the tumors. A(1)ARs were prominently expressed in microglia associated with tumor cells as revealed with immunocytochemistry but low in microglia in the unaffected brain tissue. The A(1)AR could also be detected on microglia from human glioblastoma resections. To study functional interactions between tumor and host cells, we studied glioblastoma growth in organotypical brain slice cultures. A(1)AR agonists suppressed tumor growth. When, however, microglial cells were depleted from the slices, the agonists even stimulated tumor growth. Thus, adenosine attenuates glioblastoma growth acting via A(1)AR in microglia.
authors.affiliation: Cellular Neuroscience Group, Max Delbrück Center for Molecular Medicine, Berlin, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Synowitz
authors.forename: Rainer
authors.initials: R
authors.lastname: Glass
authors.forename: Katrin
authors.initials: K
authors.lastname: Färber
authors.forename: Darko
authors.initials: D
authors.lastname: Markovic
authors.forename: Golo
authors.initials: G
authors.lastname: Kronenberg
authors.forename: Ken
authors.initials: K
authors.lastname: Herrmann
authors.forename: Juergen
authors.initials: J
authors.lastname: Schnermann
authors.forename: Christiane
authors.initials: C
authors.lastname: Nolte
authors.forename: Nico
authors.initials: N
authors.lastname: van Rooijen
authors.forename: Juergen
authors.initials: J
authors.lastname: Kiwit
authors.forename: Helmut
authors.initials: H
authors.lastname: Kettenmann
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division; D002478:Cells, Cultured; D017930:Genes, Reporter; D005838:Genotype; D005909:Glioblastoma / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D017628:Microglia / Q000166:cytology* / Q000502:physiology; D043682:Receptor, Adenosine A1 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
chemical_list: D043682:Receptor, Adenosine A1; D000241:Adenosine