Production of tropane alkaloids by small-scale bubble column bioreactor cultures of Scopolia parviflora adventitious roots.
The mass production of tropane alkaloids from adventitious root cultures of Scopolia parviflora, in small-scale bubble column bioreactor (BCB) was attempted. Adventitious roots of S. parviflora produced relatively enhanced levels of scopolamine and hyoscyamine in bioreactor compared to flask type cultures, and rapidly produced root clumps, with continuously increasing biomass throughout the culture period. The production of scopolamine and hyoscyamine in the top and bottom regions of root clumps were higher than in the core region. The adventitious root cultures of S. parviflora in the BCB required a relatively high level of aeration. The optimized conditions for the bioreactor culture growth and alkaloid production were found to be 3g of inoculum, on a fresh weight basis, a 15-day culture period and 0.4vvm of airflow. The elicitation by Staphylococus aureus increased the specific compound of scopolamine, while the production of hyoscyamine was slightly inhibited in BCB cultures.
Min
Ji Yun
JY
Division of Environmental Forest Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Jung
Hee Young
HY
Kang
Seung Mi
SM
Kim
Yong Duck
YD
Kang
Young Min
YM
Park
Dong Jin
DJ
Prasad
Doddananjappa Theertha
DT
Choi
Myung Suk
MS
D000470:Alkaloids / Q000096:biosynthesis*; D001285:Atropine / Q000096:biosynthesis; D018533:Biomass; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods; D007700:Kinetics; D018517:Plant Roots / Q000378:metabolism*; D012601:Scopolamine / Q000096:biosynthesis; D032603:Scopolia / Q000378:metabolism*; D014326:Tropanes / Q000378:metabolism*
D000470:Alkaloids; D014326:Tropanes; D001285:Atropine; D012601:Scopolamine