title: Co-administration of zinc and n-acetylcysteine prevents arsenic-induced tissue oxidative stress in male rats.
abstract: Arsenic is a widespread environmental toxicant that may cause neuropathy, skin lesions, vascular lesions and cancer upon prolonged exposure. Improving nourishment like supplementation of micronutrients, antioxidants, vitamins and amino acids could be able to halve the risk in those who were previously the poor nourished. The present study was planned to investigate the preventive effects of zinc and n-acetylcysteine (NAC) supplementation either alone or in combination with arsenic on selected biochemical variables indicative of oxidative stress and liver injury in male rats. For 3 weeks 25 male wistar rats were exposed to arsenic as sodium arsenite (2 mg/kg, orally through gastric intubation) either alone or in combination with NAC (10 mg/kg, intraperitoneally), zinc (5 mg/kg, orally) or zinc plus NAC. Animals were sacrificed 24h after the last dosing for various biochemical parameters. Concomitant administration of zinc with arsenic showed remarkable protection against blood delta-aminolevulinic acid dehydratase (ALAD) activity as well as providing protection to hepatic biochemical variables indicative of oxidative stress (like thiobarbituric acid reactive substances (TBARS) level, catalase) and tissue injury. NAC supplementation on the other hand, was moderately effective in protecting animals from the toxic effects of arsenic. Interestingly, concomitant administration of zinc and NAC was most effective compared to zinc or NAC in eliciting above-mentioned protective effects. The above results suggest significant protective value of combined zinc and NAC administration in acute arsenic exposure.
authors.affiliation: Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Modi
authors.forename: Ramesh K
authors.initials: RK
authors.lastname: Kaul
authors.forename: Gurusamy M
authors.initials: GM
authors.lastname: Kannan
authors.forename: Swaran J S
authors.initials: SJ
authors.lastname: Flora
mesh_terms: D000111:Acetylcysteine / Q000008:administration & dosage*; D000284:Administration, Oral; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001151:Arsenic / Q000037:antagonists & inhibitors / Q000493:pharmacokinetics / Q000633:toxicity*; D018053:Arsenites / Q000633:toxicity; D001219:Aspartate Aminotransferases / Q000097:blood; D004357:Drug Synergism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D000623:Porphobilinogen Synthase / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D017670:Sodium Compounds / Q000633:toxicity; D014018:Tissue Distribution; D015032:Zinc / Q000008:administration & dosage* / Q000097:blood
chemical_list: D018053:Arsenites; D017670:Sodium Compounds; C017947:sodium arsenite; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000623:Porphobilinogen Synthase; D015032:Zinc; D001151:Arsenic; D000111:Acetylcysteine