title: Detection of organics using porphyrin embedded nanoporous organosilicas.
abstract: Molecularly imprinted polymers and silica have been studied as receptor binding site mimics for use in a wide range of separation, catalysis, and detection applications employing transduction mechanisms including conductometric, amperometric, and capacitance. Porphyrins are also well known as sensor components due to the extreme sensitivity of their spectrophotometric characteristics to changes in their immediate environment. We have developed periodic mesoporous organosilicas (PMO) which incorporate a porphyrin into the material for use as an optical indicator of target binding. This material combines the stability, selectivity, and high density of binding sites characteristic of the molecularly imprinted PMO with the sensitivity and selectivity of the porphyrin. We demonstrate binding of p-nitrophenol, p-cresol, 2,4,6-trinitrotoluene, and RDX by the porphyrin-embedded PMOs with selective adsorption of TNT over the other analytes. In addition, the binding of each of the organics by the PMO results in unique changes in the spectrophotometric characteristics of the incorporated porphyrin. These changes can be observed by visual inspection or through the use of fluorescence spectra collected in 96-well format.
authors.affiliation: Center for Bio/Molecular Science & Engineering, Code 6900, Naval Research Laboratory, Washington, DC 20375-5348, USA.
authors.forename: Brandy
authors.initials: B
authors.lastname: Johnson-White
authors.forename: Mazyar
authors.initials: M
authors.lastname: Zeinali
authors.forename: Kara M
authors.initials: KM
authors.lastname: Shaffer
authors.forename: Charles H
authors.initials: CH
authors.lastname: Patterson
authors.forename: Paul T
authors.initials: PT
authors.lastname: Charles
authors.forename: Michael A
authors.initials: MA
authors.lastname: Markowitz
mesh_terms: D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009930:Organic Chemicals / Q000032:analysis* / Q000737:chemistry; D010316:Particle Size; D016062:Porosity; D011166:Porphyrins / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013499:Surface Properties
chemical_list: D009930:Organic Chemicals; D011166:Porphyrins; D012822:Silicon Dioxide