title: Electron paramagnetic resonance studies of an integral membrane peptide inserted into aligned phospholipid bilayer nanotube arrays.
abstract: This communication reports for the first time the determination of the helical tilt of an integral membrane peptide inserted into aligned phospholipids bilayer nanotube arrays using spin label EPR spectroscopy. Also, we demonstrate herein how the helical tilt of the peptide can be easily calculated using the hyperfine splitting values gleaned from a perpendicularly aligned bilayer in phospholipid bilayer nanotube arrays. EPR spectral simulations were used to verify the method.
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio 45056, USA.
authors.forename: Ethan S
authors.initials: ES
authors.lastname: Karp
authors.forename: Johnson J
authors.initials: JJ
authors.lastname: Inbaraj
authors.forename: Mikhail
authors.initials: M
authors.lastname: Laryukhin
authors.forename: Gary A
authors.initials: GA
authors.lastname: Lorigan
mesh_terms: D003497:Cyclic N-Oxides / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011950:Receptors, Cholinergic / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D003497:Cyclic N-Oxides; D008051:Lipid Bilayers; D011950:Receptors, Cholinergic; D013113:Spin Labels; D004134:Dimyristoylphosphatidylcholine; C037109:2,2,6,6-tetramethylpiperidine-N-oxide-4-amino-4-carboxylic acid