Soluble factors from neuronal cultures induce a specific proliferation and resistance to apoptosis of cognate mouse skeletal muscle precursor cells.
The mechanisms or the physiological events, which control the regeneration of skeletal muscle through muscle precursor cell multiplication and differentiation, are still largely unknown. To address the question of the involvement of neurons in this process, skeletal muscle progenitors were grown in the presence of conditioned media obtained from 3-day-old cultures of embryonic neurons (derived from either the dorsal or the ventral region of 11-day-old mouse embryos) or media conditioned with satellite cells. Strikingly, only satellite cells cultured in medium conditioned from ventral embryonic neurons exhibited increased proliferation, as well as resistance to staurosporine (STS)-induced apoptosis. Our results suggest the existence of specific anti-apoptogenic neural soluble signals, which could be involved in skeletal muscle regeneration pathways.
Pelletier
Maude
M
INSERM UMR 601, 9, Quai Moncousu, Nantes, France.
Rossignol
Julien
J
Oliver
Lisa
L
Zampieri
Maryvonne
M
Fontaine-Pérus
Josiane
J
Vallette
François M
FM
Lescaudron
Laurent
L
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001973:Bromodeoxyuridine / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004622:Embryo, Mammalian; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology*; D032446:Myoblasts / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000737:chemistry* / Q000187:drug effects; D019311:Staurosporine / Q000494:pharmacology; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology*
D017077:Culture Media, Conditioned; D004791:Enzyme Inhibitors; D005904:Glial Fibrillary Acidic Protein; D020169:Caspases; D001973:Bromodeoxyuridine; D019311:Staurosporine