title: Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning.
abstract: We report a method for the identification and quantification of glycerophospholipid molecular species that is based on the simultaneous automated acquisition and processing of 41 precursor ion spectra, specific for acyl anions of common fatty acids moieties and several lipid class-specific fragment ions. Absolute quantification of identified species was linear within a concentration range of 10 nM-100 microM and was achieved by spiking into total lipid extracts a set of synthetic lipid standards with diheptadecanoyl (17:0/17:0) fatty acid moieties, representing six common classes of glycerophospholipids. The automated analysis of total lipid extracts was powered by a robotic nanoflow ion source and produced currently the most detailed description of the glycerophospholipidome.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
authors.forename: Christer S
authors.initials: CS
authors.lastname: Ejsing
authors.forename: Eva
authors.initials: E
authors.lastname: Duchoslav
authors.forename: Julio
authors.initials: J
authors.lastname: Sampaio
authors.forename: Kai
authors.initials: K
authors.lastname: Simons
authors.forename: Ron
authors.initials: R
authors.lastname: Bonner
authors.forename: Christoph
authors.initials: C
authors.lastname: Thiele
authors.forename: Kim
authors.initials: K
authors.lastname: Ekroos
authors.forename: Andrej
authors.initials: A
authors.lastname: Shevchenko
mesh_terms: D005227:Fatty Acids; D020404:Glycerophospholipids / Q000032:analysis* / Q000737:chemistry*; D007477:Ions / Q000737:chemistry*; D007554:Isotopes; D012985:Software Design; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D020404:Glycerophospholipids; D007477:Ions; D007554:Isotopes