Novel intermediates of acenaphthylene degradation by Rhizobium sp. strain CU-A1: evidence for naphthalene-1,8-dicarboxylic acid metabolism.
The acenaphthylene-degrading bacterium Rhizobium sp. strain CU-A1 was isolated from petroleum-contaminated soil in Thailand. This strain was able to degrade 600 mg/liter acenaphthylene completely within three days. To elucidate the pathway for degradation of acenaphthylene, strain CU-A1 was mutagenized by transposon Tn5 in order to obtain mutant strains deficient in acenaphthylene degradation. Metabolites produced from Tn5-induced mutant strains B1, B5, and A53 were purified by thin-layer chromatography and silica gel column chromatography and characterized by mass spectrometry. The results suggested that this strain cleaved the fused five-membered ring of acenaphthylene to form naphthalene-1,8-dicarboxylic acid via acenaphthenequinone. One carboxyl group of naphthalene-1,8-dicarboxylic acid was removed to form 1-naphthoic acid which was transformed into salicylic acid before metabolization to gentisic acid. This work is the first report of complete acenaphthylene degradation by a bacterial strain.
Poonthrigpun
Siriwat
S
Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Pattaragulwanit
Kobchai
K
Paengthai
Sarunya
S
Kriangkripipat
Thanyanuch
T
Juntongjin
Kanchana
K
Thaniyavarn
Suthep
S
Petsom
Amorn
A
Pinphanichakarn
Pairoh
P
D000073:Acenaphthenes / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D005798:Genes, Bacterial; D008956:Models, Chemical; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016254:Mutagenesis, Insertional; D009281:Naphthalenes / Q000737:chemistry / Q000378:metabolism*; D010578:Petroleum; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D013785:Thailand
D000073:Acenaphthenes; D009281:Naphthalenes; D010578:Petroleum; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C042553:acenaphthylene; C073252:1,8-naphthalenedicarboxylic acid