title: Trichinella: differing effects of antigens from encapsulated and non-encapsulated species on in vitro nitric oxide production.
abstract: Trichinellosis is a cosmopolitan zoonotic disease affecting a wide variety of animals, including man. Non-encapsulated and encapsulated species diverge with respect to their developmental strategies. Little is known at the molecular level about parasite-derived mediators responsible for host muscle cell transformation occurring during trichinellosis. In this context, host-parasite relationships in Trichinella-infected animals could be related to different host-immune and cell mediators, e.g. nitric oxide (NO). Here, we investigate the stimulatory/inhibitory role of L1 antigens from four encapsulated (T. spiralis, T. britovi, T. nelsoni and T. nativa) and one non-encapsulated (T. pseudospiralis) Trichinella species on NO production from rat macrophages in vitro. Our results demonstrate that encapsulated and non-encapsulated Trichinella species differ in their capacity to stimulate the secretion of NO from host macrophages. Biological significance of these differences should be further assessed in the available experimental models.
authors.affiliation: Laboratorio de Inmunología Parasitaria y Molecular, CISET, Facultad de Farmacia, Universidad de Salamanca, Avda. Campo Charro, s/n 37007, Salamanca, Spain.
authors.forename: M Amparo
authors.initials: MA
authors.lastname: Andrade
authors.forename: Mar
authors.initials: M
authors.lastname: Siles-Lucas
authors.forename: Julio
authors.initials: J
authors.lastname: López-Abán
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Nogal-Ruiz
authors.forename: José Luis
authors.initials: JL
authors.lastname: Pérez-Arellano
authors.forename: Antonio R
authors.initials: AR
authors.lastname: Martínez-Fernández
authors.forename: Antonio
authors.initials: A
authors.lastname: Muro
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002478:Cells, Cultured; D005838:Genotype; D006790:Host-Parasite Interactions; D006801:Humans; D066298:In Vitro Techniques; D007411:Intestinal Diseases, Parasitic / Q000276:immunology / Q000469:parasitology / Q000662:veterinary; D016676:Macrophages, Alveolar / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D051381:Rats; D013045:Species Specificity; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D000947:Antigens, Helminth; D009569:Nitric Oxide