title: Protein profiling and transcript expression levels of heat shock proteins in 17beta-estradiol-treated human MCF-7 breast cancer cells.
abstract: Using proteomics, proteins regulated by 17beta-estradiol (E2) were identified in human MCF-7 breast cancer cells; 26 proteins including heat shock proteins (Hsps) were differentially regulated by E2 in cells cultured in serum-free condition. When the transcript levels of these proteins and another Hsps were measured by real-time PCR, the transcripts encoding 6 proteins (Hsp56, Hsp90alpha, Hsp110, protein disulfide isomerase related protein, XTP3-transactivated protein A and stathmin 1) were significantly up-regulated and that encoding aminoacylase 1 was down-regulated by E2 in cells cultured with or without serum. The protein profiling and transcript expression patterns of E2-regulated proteins including Hsps in MCF-7 cells suggested the involvement of these proteins in breast carcinogenesis. Proteins (or transcripts) whose expression pattern is altered by E2 might be potent targets for treating breast cancer patients.
authors.affiliation: Laboratory of Chemical Genomics, Bio-Organic Science Division, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong-gu, Daejeon 305-600, South Korea.
authors.forename: Su-Ui
authors.initials: SU
authors.lastname: Lee
authors.forename: Bum Tae
authors.initials: BT
authors.lastname: Kim
authors.forename: Yong Ki
authors.initials: YK
authors.lastname: Min
authors.forename: Seong Hwan
authors.initials: SH
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006360:Heat-Shock Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D040901:Proteomics; D044967:Serum / Q000502:physiology
chemical_list: D006360:Heat-Shock Proteins; D004958:Estradiol