Photophysics of ANS. III: Circular dichroism of ANS and anilinonaphthalene in I-FABP.
We investigate the circular dichroism of the I-FABP system with the ligands ANS (1,8-anilinonaphthalene sulfonate) and AnN (anilinonaphthalene) as previously reported in our earlier publications in the series (referred to as I and II here) on ANS photophysics. We employ our semi-empirical calculated spectral functions (from II) to compute the actual CD spectra, without any additional assumptions or data except what we have previously presented with respect to binding geometry (in I). The common mechanisms fail to produce the observed spectra. However, we identify a novel mechanism of induced CD activity, which does succeed. This new mechanism also suggests how it is that near UV CD can often show extreme sensitivity to local 'order' effects.
Kirk
William
W
Mayo Clinic School of Medicine Rochester, MN 55905, USA. kirk.william@mayo.edu
Klimtchuk
Elena
E
D015057:1-Naphthylamine / Q000031:analogs & derivatives* / Q000737:chemistry; D000595:Amino Acid Sequence; D000817:Anilino Naphthalenesulfonates / Q000737:chemistry*; D002942:Circular Dichroism / Q000379:methods*; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D008956:Models, Chemical; D009154:Mutation; D010777:Photochemistry; D055585:Physical Phenomena; D010825:Physics; D011485:Protein Binding; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000737:chemistry / Q000235:genetics; D014443:Tyrosine / Q000737:chemistry
D000817:Anilino Naphthalenesulfonates; D050556:Fatty Acid-Binding Proteins; D014443:Tyrosine; C027132:1-anilino-8-naphthalenesulfonate; D014364:Tryptophan; C005444:N-phenyl-1-naphthylamine; D015057:1-Naphthylamine