title: [Increased sensitivity to chemotherapeutic drug by RNAi targeting Hsp701A in HepG2 cells].
abstract: OBJECTIVE
  To investigate the effects on chemotherapeutic sensitivity to the drugs of tumor cells induced by Hsp701A targeting siRNA expression vector.
  
  
  METHODS
  Specific small interference RNA (siRNA) targeting Hsp701A gene was generated and its eukaryotic expression vector was constructed and transfected into human HepG2 cells.Sensitivity to the chemotherapeutic drug of HepG2 cells transfected with the vector was detected by MTT colorimetry.
  
  
  RESULTS
  The eukaryotic expression vector of siRNA targeting Hsp701A was successfully constructed and transfected into human HepG2 cells. Sensitivity to the chemotherapeutic drug of HepG2 cells was increased after Hsp701A was knock down (P<0.05).
  
  
  CONCLUSIONS
  Knockdown of Hsp701A gene by siRNA enhances sensitivity to chemotherapeutic drug in HepG2 cells.
authors.affiliation: Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.forename: Wang-gang
authors.initials: WG
authors.lastname: Zhang
authors.forename: Li-feng
authors.initials: LF
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: An-gang
authors.initials: AG
authors.lastname: Yang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004262:DNA Restriction Enzymes / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D050884:HSP72 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D050884:HSP72 Heat-Shock Proteins; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes