Cynomolgus monkey embryonic stem cell lines express green fluorescent protein.
We successfully established cynomolgus monkey embryonic stem (cES) cell lines expressing enhanced green fluorescent protein (GFP) by introducing a GFP-encoding gene under cytomegalovirus immediate early enhancer (CMVIE) promoter regulation into cES cells. The cells maintained the ability of in vitro differentiation toward ectodermal, mesodermal, and endodermal lineages, and produced teratomas composed of tissues derived from the three embryonic germ layers when transplanted into severe combined immunodeficient disease mice. GFP expression was also observed in the differentiated cells. These GFP-expressing cES cell lines are considered useful for basic research, including cell transplantation.
Ueda
Shigehiko
S
Department of Gastroenterology and Hepatology, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Yoshikawa
Masahide
M
Ouji
Yukiteru
Y
Saito
Ko
K
Moriya
Kei
K
Nishiofuku
Mariko
M
Hayashi
Noriko
N
Ishizaka
Shigeaki
S
Shimada
Keiji
K
Konishi
Noboru
N
Fukui
Hiroshi
H
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008252:Macaca fascicularis; D051379:Mice; D016513:Mice, SCID; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins