title: Survival of Escherichia coli O157:H7 in wastewater from dairy lagoons.
abstract: OBJECTIVE
  To determine the survival of Escherichia coli O157:H7 in dairy wastewater from on-site holding lagoons equipped with or without circulating aerators.
  
  
  RESULTS
  Survival was monitored in dairy lagoon microcosms equipped with or without scale-size circulators. Both laboratory strains of E. coli O157:H7 and an isolate of E. coli H7 from wastewater had poor survival rates and none proliferated in water from waste lagoons with or without circulators. Furthermore, the decline of E. coli O157:H7 was not enhanced in those microcosms equipped with circulators. Strain variation in survival was observed in both circulated and settling waters. The decline rate of E. coli O157:H7 Odwalla strain increased proportionately with the inoculum load. Escherichia coli failed to establish itself in wastewater even after four sequential inoculations simulating continuous faecal input into the lagoon. The native aerobic bacteria survived longer with a decimal reduction time of 21.3 days vs either introduced or native E. coli, which declined rapidly with decimal reduction time of 0.5-9.4 days.
  
  
  CONCLUSIONS
  Escherichia coli O157:H7 failed to establish and proliferate in dairy wastewater microcosms equipped with or without circulating aerators.
  
  
  CONCLUSIONS
  This study furthers our knowledge of pathogen survival in wastewater, and suggests that proper management of wastewater before its use in irrigation is essential to reduce pathogen transfer to crops.
authors.affiliation: USDA, Agriculture Research Service, Western Regional Research Center, Foodborne Contaminants Research Unit, Albany, CA 94710, USA. subba@pw.usda.gov
authors.forename: S V
authors.initials: SV
authors.lastname: Ravva
authors.forename: C Z
authors.initials: CZ
authors.lastname: Sarreal
authors.forename: B
authors.initials: B
authors.lastname: Duffy
authors.forename: L H
authors.initials: LH
authors.lastname: Stanker
mesh_terms: D000064:Acclimatization; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D003612:Dairying; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D007220:Industrial Waste; D008372:Manure / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D012293:Rifampin / Q000494:pharmacology; D012621:Seasons; D013045:Species Specificity; D013997:Time Factors; D018505:Waste Management / Q000379:methods*; D014871:Water Microbiology
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D007220:Industrial Waste; D008372:Manure; D012293:Rifampin