Application of microfluidic chip with integrated optics for electrophoretic separations of proteins.
This paper describes the fabrication, the characterization and the applications of a capillary electrophoresis microchip. This hybrid device (glass/PDMS) features channels and optical waveguides integrated in one common substrate. It can be used for electrophoretic separation and fluorimetric detection of molecules. The microfluidic performance of the device is demonstrated by capillary zone and gel electrophoresis of proteins.
Vieillard
Julien
J
LEOM-ECL (UMR CNRS no. 5512), 36, av. G. de Collongue, 69134 Ecully, France. Julien.vieillard@ec-lyon.fr
Mazurczyk
Radoslaw
R
Morin
Christophe
C
Hannes
Benjamin
B
Chevolot
Yann
Y
Desbène
Paul-Louis
PL
Krawczyk
Stanislas
S
D002232:Carbocyanines / Q000302:isolation & purification; D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D055095:Optics and Photonics / Q000295:instrumentation*; D011506:Proteins / Q000302:isolation & purification*; D015203:Reproducibility of Results; D019809:Streptavidin / Q000302:isolation & purification
D002232:Carbocyanines; D011506:Proteins; C089187:cyanine dye 3; D019809:Streptavidin