Variants in mitochondrial tRNAGlu, tRNAArg, and tRNAThr may influence the phenotypic manifestation of deafness-associated 12S rRNA A1555G mutation in three Han Chinese families with hearing loss.
We report here on the clinical, genetic, and molecular characterization of three Han Chinese pedigrees with aminoglycoside-induced and nonsyndromic hearing loss. Clinical evaluation revealed the variable phenotype of hearing loss including severity, age-at-onset, audiometric configuration in these subjects. Penetrances of hearing loss in BJ107, BJ108, and BJ109 pedigrees are 35%, 63%, and 67%, respectively. Mutational analysis of the complete mitochondrial genomes in these pedigrees showed the identical homoplasmic A1555G mutation and distinct sets of mitochondrial DNA (mtDNA) variants belonging to haplogroups N, F, and M, respectively. Of these variants, the A14693G mutation in the tRNA(Glu), the T15908C mutation in the tRNA(Thr), and the T10454C mutation in the tRNA(Arg) are of special interest as these mutations occur at positions which are highly evolutionarily conserved nucleotides of corresponding tRNAs. These homoplasmic mtDNA mutations were absent among 156 unrelated Chinese controls. The A14693G and T10454C mutations occur at the highly conserved bases of the TpsiC-loop of tRNA(Glu) and tRNA(Arg), respectively. Furthermore, the T15908C mutation in the tRNA(Thr) disrupts a highly conserved A-U base-pairing at the D-stem of this tRNA. The alteration of structure of these tRNAs by these mtDNA mutations may lead to a failure in tRNA metabolism, thereby causing impairment of mitochondrial translation. Thus, mitochondrial dysfunctions, caused by the A1555G mutation, would be worsened by these mtDNA mutations. Therefore, these mtDNA mutations may have a potential modifier role in increasing the penetrance and expressivity of the deafness-associated 12S rRNA A1555G mutation in those Chinese pedigrees.
Young
Wie-Yen
WY
Institute of Otolaryngology, Chinese PLA General Hospital, Beijing, China.
Zhao
Lidong
L
Qian
Yaping
Y
Li
Ronghua
R
Chen
Jing
J
Yuan
Huijun
H
Dai
Pu
P
Zhai
Suoqiang
S
Han
Dongyi
D
Guan
Min-Xin
MX
D020029:Base Pairing; D001483:Base Sequence; D002681:China; D004252:DNA Mutational Analysis; D017931:DNA Primers; D005260:Female; D034381:Hearing Loss / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D010641:Phenotype; D017354:Point Mutation / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D012347:RNA, Transfer, Arg / Q000235:genetics*; D012352:RNA, Transfer, Glu / Q000235:genetics*; D012363:RNA, Transfer, Thr / Q000235:genetics*; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D012335:RNA, Ribosomal; D012347:RNA, Transfer, Arg; D012352:RNA, Transfer, Glu; D012363:RNA, Transfer, Thr; C075526:RNA, ribosomal, 12S