Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential.
A recombinant phage library harbouring Mycoplasma meleagridis (MM) genomic DNA fragments was generated in the bacteriophage lambda gt11 expression vector. The library was screened for expression of MM specific antigens with a polyclonal antiserum that had been preadsorbed with antigens of the most common unrelated avian mycoplasma species. A 49-amino acid antigenic domain unique to MM was isolated, expressed in Escherichia coli, and its serodiagnostic potential was demonstrated. An antiserum raised against this MM-specific antigenic domain recognized a cluster of seven membrane-associated MM proteins with molecular masses ranging from 34 to 75 kDa. Overall, this study resulted in the identification of a potent serodiagnostic tool and revealed the complex antigenic nature of MM.
Mardassi
B Ben Abdelmoumen
BB
Laboratoire des Mycoplasmes, Institut Pasteur de Tunis, 13 Place Pasteur, B.P. 74, 1002 Tunis-Belvédère, Tunis, Tunisia. Boutheina.mardassi@pasteur.rns.tn
Béjaoui Khiari
A
A
Oussaief
L
L
Landoulsi
A
A
Brik
C
C
Mlik
B
B
Amouna
F
F
D000818:Animals; D000907:Antibodies, Bacterial / Q000378:metabolism; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D007106:Immune Sera / Q000378:metabolism; D015151:Immunoblotting / Q000379:methods; D008969:Molecular Sequence Data; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary; D045794:Mycoplasma meleagridis / Q000235:genetics* / Q000276:immunology*; D011201:Poultry Diseases / Q000175:diagnosis / Q000382:microbiology; D013045:Species Specificity; D014422:Turkeys
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D007106:Immune Sera