title: Molecular epidemiology of drug-resistant Salmonella Typhimurium in Spain.
abstract: One hundred and forty-seven Salmonella serotype Typhimurium strains isolated in three provinces in the midwest of Spain were studied. Of these, 93.6% were drug resistant. There were two predominant resistance phenotypes: 43 isolates (29.3%) were resistant to amoxicillin, tetracyclines, chloramphenicol, streptomycin and sulphamethoxazole and 27 isolates (18.4%) to amoxicillin, amoxicillin/clavulanic acid, tetracyclines, chloramphenicol, streptomycin and sulphamethoxazole. Randomly amplified polymorphic DNA (RAPD) analysis and pulsed field gel electrophoresis (PFGE) were performed for molecular typing. Thirty-six DNA band profiles were differentiated by RAPD, and 38 by PFGE. We found a high level of clonality; 27% of strains were identical by both methods. There were additional smaller clonal lines within every area. The highest discriminatory power was obtained with PFGE, but the greatest degree of genetic diversity was observed among Salmonella Typhimurium using both RAPD and PFGE.
authors.affiliation: Departamento de Medicina Preventiva, Salud Pública y Microbiología Médica, Universidad de Salamanca, and Departamento de Microbiología, Hospital Universitario de Salamanca, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Delgado Ronda
authors.forename: J L
authors.initials: JL
authors.lastname: Muñoz Bellido
authors.forename: M I
authors.initials: MI
authors.lastname: García García
authors.forename: R
authors.initials: R
authors.lastname: Ibáñez Pérez
authors.forename: S
authors.initials: S
authors.lastname: Muñoz Criado
authors.forename: R
authors.initials: R
authors.lastname: Serrano Heranz
authors.forename: M C
authors.initials: MC
authors.lastname: Sáenz González
authors.forename: J A
authors.initials: JA
authors.lastname: García Rodríguez
mesh_terms: D024881:Drug Resistance, Bacterial; D006801:Humans; D012480:Salmonella Infections / Q000453:epidemiology* / Q000382:microbiology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D013030:Spain / Q000453:epidemiology