title: pH-dependent activation of the BarA-UvrY two-component system in Escherichia coli.
abstract: The barA and uvrY genes of Escherichia coli encode a two-component sensor kinase and a response regulator, respectively. Although this system plays a major role in the regulation of central carbon metabolism, motility, and biofilm formation by controlling the expression of the CsrB and CsrC noncoding RNAs, the environmental conditions and the physiological signal(s) to which it responds remain obscure. In this study, we explored the effect of external pH on the activity of BarA/UvrY. Our results indicate that a pH lower than 5.5 provides an environment that does not allow activation of the BarA/UvrY signaling pathway.
authors.affiliation: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 México D.F., México.
authors.forename: Verónica
authors.initials: V
authors.lastname: Mondragón
authors.forename: Bernardo
authors.initials: B
authors.lastname: Franco
authors.forename: Kristina
authors.initials: K
authors.lastname: Jonas
authors.forename: Kazushi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Tony
authors.initials: T
authors.lastname: Romeo
authors.forename: Ojar
authors.initials: O
authors.lastname: Melefors
authors.forename: Dimitris
authors.initials: D
authors.lastname: Georgellis
mesh_terms: D004926:Escherichia coli / Q000254:growth & development / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D014157:Transcription Factors; C419603:UvrY protein, E coli; C074276:barA protein, E coli; D010770:Phosphotransferases