title: Stimulation of human monocytes with the gram-positive vaccine vector Streptococcus gordonii.
abstract: Streptococcus gordonii is a bacterial vaccine vector which has previously been shown to activate dendritic cells in vitro and to induce local and systemic immune responses in vivo. In the present study, human monocytes (THP-1 cell line and peripheral blood monocytes) were characterized following interaction with S. gordonii. Treatment of human monocytes with S. gordonii but not latex beads induced a clear up-regulation of CD83, CD40, CD80, and CD54 and the down-regulation of CD14. Furthermore, bacterial treatment stimulated an increased expression of Toll-like receptor 5 (TLR5), TLR6, and TLR7, production of the proinflammatory cytokines tumor necrosis factor alpha and interleukin 1 beta, and reduction of the phagocytic activity. This work shows that the immunostimulatory activity of S. gordonii is not restricted to induction of dendritic-cell maturation but also affects the differentiation process of human monocytes.
authors.affiliation: Laboratorio di Microbiologia Molecolare e Biotecnologia, LA.M.M.B., Dipartimento di Biologia Molecolare, Università di Siena, Policlinico Le Scotte, Viale Bracci, 53100 Siena, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Ciabattini
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Cuppone
authors.forename: Rita
authors.initials: R
authors.lastname: Pulimeno
authors.forename: Francesco
authors.initials: F
authors.lastname: Iannelli
authors.forename: Gianni
authors.initials: G
authors.lastname: Pozzi
authors.forename: Donata
authors.initials: D
authors.lastname: Medaglini
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D018122:B7-1 Antigen / Q000032:analysis; D019013:CD40 Antigens / Q000032:analysis; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D015964:Gene Expression Regulation, Bacterial / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007375:Interleukin-1 / Q000096:biosynthesis; D007948:Leukemia, Monocytic, Acute / Q000473:pathology; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D010587:Phagocytosis; D013291:Streptococcus / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000276:immunology*; D013997:Time Factors; D051198:Toll-Like Receptor 5 / Q000276:immunology; D051216:Toll-Like Receptor 6 / Q000276:immunology; D051199:Toll-Like Receptor 7 / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D019013:CD40 Antigens; D007375:Interleukin-1; D018950:Lipopolysaccharide Receptors; D051198:Toll-Like Receptor 5; D051216:Toll-Like Receptor 6; D051199:Toll-Like Receptor 7; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1