Alteration of cystatin C in the cerebrospinal fluid of multiple sclerosis.
The protein profiles in the cerebrospinal fluid of 10 patients with multiple sclerosis (MS), 10 patients with neuromyelitis optica (NMO), 8 inflammatory disease control patients, and 4 noninflammatory disease control patients were screened by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Peaks of 12.5 kDa were significantly lower in multiple sclerosis, NMO, and inflammatory disease control patients than in noninflammatory disease control patients, and 13.4 kDa peaks were higher in NMO than in inflammatory disease control patients. Further analyses demonstrated that both peaks were cystatin C. Enzyme-linked immunosorbent assay showed that the cystatin C levels tended to be lower in multiple sclerosis and NMO. Alterations of cystatin C may relate to the pathogeneses of demyelinating diseases.
Nakashima
Ichiro
I
Department of Neurology, Tohoku University School of Medicine, Sendai, Japan. inakashima@em.neurol.med.tohoku.ac.jp
Fujinoki
Masakatsu
M
Fujihara
Kazuo
K
Kawamura
Takeshi
T
Nishimura
Toshihide
T
Nakamura
Masashi
M
Itoyama
Yasuto
Y
D055316:Cystatin C; D015891:Cystatins / Q000134:cerebrospinal fluid* / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D047468:Immunoprecipitation; D008970:Molecular Weight; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000134:cerebrospinal fluid*; D009471:Neuromyelitis Optica / Q000134:cerebrospinal fluid*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
C525030:CST3 protein, human; D055316:Cystatin C; D015891:Cystatins