Identification and differentiation of Legionella pneumophila and Legionella spp. with real-time PCR targeting the 16S rRNA gene and species identification by mip sequencing.
Fluorescent resonance energy transfer probes targeting the 16S rRNA gene were constructed for a sensitive and specific real-time PCR for identification and differentiation of Legionella pneumophila from other Legionella spp. For identification of non-L. pneumophila spp. by direct amplicon sequencing, two conventional PCR assays targeting the mip gene were established.
Stølhaug
Anne
A
Department of Medical Microbiology, Laboratory Center, St. Olavs Hospital, N-7006 Trondheim, Norway.
Bergh
Kåre
K
D001426:Bacterial Proteins; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D020104:Immunophilins / Q000235:genetics*; D007875:Legionella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016952:Legionella pneumophila / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D007877:Legionnaires' Disease / Q000175:diagnosis; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D019696:Peptidylprolyl Isomerase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D008565:Membrane Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D020104:Immunophilins; C512922:Mip protein, Legionella pneumophila; D019696:Peptidylprolyl Isomerase