Physiologic roles of 11beta-hydroxysteroid dehydrogenase type 2 in kidney.
We developed enzyme-linked immunosorbent assays to measure urinary free cortisone (E) and cortisol (F) and analyzed correlations between clinical measures reflecting mineralocorticoid action and 24-hour urinary excretion of E and F or their ratio, uE/F, which has been considered as the most sensitive index of renal 11beta-hydroxysteroid dehydrogenase type 2 activity. Two hundred nineteen healthy men were enrolled in this study. The uE/F ratio was 1.10 +/- 0.41 (mean +/- SD), and a strong linear correlation between uE and uF was observed in a double reciprocal plot. Urinary acid-labile aldosterone excretion had a negative correlation with 24-hour urinary Na excretion and Na/K ratio, but uE/F ratio had a weak positive correlation with the Na/K ratio and no significant correlation with 24-hour urinary Na excretion. In contrast, uE and uF had positive correlations with 24-hour urinary excretions of Na and K, raising the possibility of separate renal effects mediated by the glucocorticoid receptor. Furthermore, uE and uE/F ratio had strong negative correlations with urinary concentrations of Na and K. These results suggest that renal 11beta-hydroxysteroid dehydrogenase type 2 is an important regulatory factor of renal Na and K handlings independently of and/or complementary to the mineralocorticoid action of aldosterone.
Isomura
Yukinori
Y
Department of Diabetes and Endocrinology, Gifu University School of Medicine, Gifu 501-1194, Japan.
Mune
Tomoatsu
T
Morita
Hiroyuki
H
Suwa
Tetsuya
T
Takada
Nobuki
N
Yamamoto
Yoritsuna
Y
Takeda
Jun
J
D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000502:physiology*; D000328:Adult; D000450:Aldosterone / Q000097:blood; D003348:Cortisone / Q000652:urine; D003404:Creatinine / Q000097:blood; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006854:Hydrocortisone / Q000652:urine; D007668:Kidney / Q000201:enzymology* / Q000502:physiology*; D008297:Male; D008901:Mineralocorticoids / Q000502:physiology; D014882:Water-Electrolyte Balance / Q000502:physiology
D008901:Mineralocorticoids; D000450:Aldosterone; D003404:Creatinine; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2; D003348:Cortisone; D006854:Hydrocortisone