title: Expression of p16(INK4a) in relation to histopathology and viral load of 'high-risk' HPV types in cervical neoplastic lesions.
abstract: A total of 91 cervical archival biopsy series were analysed for the presence and viral load of 'high-risk' types of human papillomavirus (HR-HPV), and p16(INK4a) expression. The women had various degrees of CIN (cervical intraepithelial neoplasia). HPV 16 was the most prevalent type found, at 47% frequency. The frequency of HPV 16 increased with increasing immunoreactivity to p16(INK4a), from 39% to 44% at cases scored low to medium, to 65% at high reactivity. Thirty (33%) of the samples had negative p16(INK4a) analysis results, but were positive for HR-HPV. There was no significant correlation between viral load and the level of p16(INK4a) expression, while the grade of CIN correlated to such expressions. Thus, p16(INK4a) expression analysis yielded information which is consistent with results from the histopathology and might complement the HPV analysis in a clinical prognostic procedure in order to find women at risk for cervical cancer.
authors.affiliation: Institute for Clinical Science, Division of Obstetrics and Gynaecology, Karolinska University Hospital, Huddinge, Karolinska Institutet, 141 86 Stockholm, Sweden. sonia.andersson@telia.com
authors.forename: Sonia
authors.initials: S
authors.lastname: Andersson
authors.forename: Darawalee
authors.initials: D
authors.lastname: Wangsa
authors.forename: Carmen
authors.initials: C
authors.lastname: Flores-Staino
authors.forename: Hamzah
authors.initials: H
authors.lastname: Safari
authors.forename: Miriam
authors.initials: M
authors.lastname: Mints
authors.forename: Anders
authors.initials: A
authors.lastname: Hjerpe
authors.forename: Bjorn
authors.initials: B
authors.lastname: Hagmar
authors.forename: Bo
authors.initials: B
authors.lastname: Johansson
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy / Q000379:methods; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000378:metabolism*; D011379:Prognosis; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000821:virology; D019562:Viral Load; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000473:pathology / Q000821:virology
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm