Numerical model for DNA loading in microdevices: stacking and autogating effects.
Many electrophoresis-based DNA sequencing and genotyping microdevices rely on field-driven effects to load and preconcentrate the sample. A quantitative model is developed for a broad class of electrophoresis-based microfabricated sample injectors. Quantitative predictions of DNA preconcentration are compared with experimental data and are shown to qualitatively reproduce the detailed time-evolving sample distribution in the injector. The model provides practical guidance on device and protocol design, in order to optimize this critical aspect of microfluidic devices.
El-Difrawy
Sameh A
SA
Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
Srivastava
Alok
A
Gismondi
Elizabeth A
EA
McKenna
Brian K
BK
Ehrlich
Daniel J
DJ
D004247:DNA / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000592:standards*; D046209:Electrophoresis, Microchip / Q000592:standards*; D017022:Flow Injection Analysis; D008956:Models, Chemical; D017422:Sequence Analysis, DNA
D004247:DNA