[Inhibition of extracts from 17 Chinese herbs on periodontal pathogenic microbes].
OBJECTIVE
To evaluate the efficiency of 17 Chinese herbs on periodontal pathogenic microbes.


METHODS
17 efficient substances from Chinese herbs were purchased from Chinese Drug Identification Bureau, including magnesium lithospermate B, magnolol, tetramethyl pyrazine, matrine, dycyrrhizin, gentiopicrin, aloperin, baicalin, oleanolic acid, ginkgo seed, total glucosides of paeony capsules, anisldehyde, archin, cablin patchouli, hydrochloric acid Berberine, forsythin, and kakonein. Antimicrobial sensitivity tests of broth microdilution methods on 96-microwell plate were carried out for identification of the antimicrobial activity of extracts against six species of microorganisms: Actinobacillus actinomycete mitans(Aa) Y4, Actinomycetes viscosus(Av) 19246, Porphyromonas gingivalis(Pg) 33277, Fusobacterium necrophorum(Fn) 25286, Actinomyces naeslundii(An) wvl 45 and Prevotella nigrescens(Pn).


RESULTS
It was found that magnesium lithospermate B and magnolol showed the most efficient inhibition on microorganism of Pn and Fn, with the MIC being 0.053 and 0.313 mg/ml for Pn and Fn, respectively. Tetramethyl pyrazine, matrine, dycyrrhizin, gentiopicrin, aloperin, baicalin, and oleanolic acid had better inhibition than total glucosides of paeony capsules, anisldehyde, archin, cablin patchouli, hydrochloric acid berberine, forsythin, and kakonein.


CONCLUSIONS
The Chinese herbs, magnesium lithospermate B and magnolol are efficient agents for inhibition against periodontal pathogenic microbes.
Zhu
Cai-lian
CL
Shanghai Research Institute of Stomatology, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University, Shanghai 200011, China.
Li
Ming-yu
MY
D000190:Actinomyces / Q000187:drug effects; D016924:Actinomyces viscosus / Q000187:drug effects; D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects; D000890:Anti-Infective Agents; D001713:Biphenyl Compounds / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005675:Fusobacterium necrophorum / Q000187:drug effects; D017705:Lignans / Q000494:pharmacology*; D010510:Periodontal Diseases / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000187:drug effects; D045242:Prevotella nigrescens / Q000187:drug effects
D000890:Anti-Infective Agents; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D017705:Lignans; C005498:magnolol; C061091:lithospermate B