title: Genetic characterization of VP3 gene of group A rotaviruses.
abstract: We sequenced VP3 gene of four rotavirus strains and examined the diversity of VP3 and its other genetic characteristics in a total of 22 Group A rotaviruses. The 22 rotavirus strains were derived from six different host species (human, avian, bovine, equine, simian and porcine). Comparison of the partial VP3 peptide sequences (aa 141-294) showed identities ranging from 49 to 99% among different species. The phylogenetic analysis of VP3 sequences revealed segregation according to the species origin. This was further confirmed by identification of three host specific domains within the VP3 peptide. Thus, the host range restriction or attenuation previously shown to associate with VP3 may be attributed to the species-specific nature of the gene.
authors.affiliation: Department of Pediatrics, Center for Diarrheal Disease Research, All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Subodh
authors.forename: Maharaj K
authors.initials: MK
authors.lastname: Bhan
authors.forename: Pratima
authors.initials: P
authors.lastname: Ray
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D002648:Child; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000821:virology*; D016415:Sequence Alignment; D013045:Species Specificity
chemical_list: D036022:Capsid Proteins; C442546:VP3 protein, Rotavirus