title: Purification, crystallization and preliminary X-ray crystallographic analysis of chitinase from Bacillus cereus NCTU2.
abstract: Chitinases (EC 3.2.1.14) are found in a broad range of organisms, including bacteria, fungi and higher plants, and play different roles depending on their origin. A chitinase from Bacillus cereus NCTU2 (ChiNCTU2) capable of hydrolyzing chitin as a carbon and nitrogen nutrient has been identified as a member of the family 18 glycoside hydrolases. ChiNCTU2 of molecular weight 36 kDa has been crystallized using the hanging-drop vapour-diffusion method. According to the diffraction of chitinase crystals at 1.10 A resolution, the crystal belongs to space group P2(1), with unit-cell parameters a = 50.79, b = 48.79, c = 66.87 A, beta = 99.31 degrees . Preliminary analysis indicates there is one chitinase molecule in the asymmetric unit, with a solvent content of 43.4%.
authors.affiliation: Life Science Group, Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
authors.forename: Chueh-Yuan
authors.initials: CY
authors.lastname: Kuo
authors.forename: Yue-Jin
authors.initials: YJ
authors.lastname: Wu
authors.forename: Yin-Cheng
authors.initials: YC
authors.lastname: Hsieh
authors.forename: Hong-Hsiang
authors.initials: HH
authors.lastname: Guan
authors.forename: Huei-Ju
authors.initials: HJ
authors.lastname: Tsai
authors.forename: Yi-Hung
authors.initials: YH
authors.lastname: Lin
authors.forename: Yen-Chieh
authors.initials: YC
authors.lastname: Huang
authors.forename: Ming-Yih
authors.initials: MY
authors.lastname: Liu
authors.forename: Yaw-Kuen
authors.initials: YK
authors.lastname: Li
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Chen
mesh_terms: D001409:Bacillus cereus / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002686:Chitin / Q000737:chemistry / Q000378:metabolism; D002688:Chitinases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D006868:Hydrolysis
chemical_list: D001426:Bacterial Proteins; D002686:Chitin; D002688:Chitinases