title: [Heterogeneity changes in secretion ability of monocyte/macrophage and its significance after trauma hemorrhage in rat].
abstract: OBJECTIVE
  To investigate the relationship between the heterogeneity in secretion ability of monocyte (Mo)/macrophage and the immune dysfunction after severe trauma.
  
  
  METHODS
  Twenty-four healthy Wistar rats were randomly divided into normal control group and trauma hemorrhage 1, 4 and 7 days groups. The contents of interleukin-6 (IL-6) and IL-10 secretion of Mo/macrophage from different anatomical regions were determined by radioimmunoassay.
  
  
  RESULTS
  (1)In normal rats, the ability to secrete IL-6 and IL-10 was different among alveolar macrophages (AM), peritoneal macrophages (PM) and Mo. PM showed the highest ability to secrete IL-10 while Mo had the highest ability to secrete IL-6. (2)After trauma hemorrhage, the secretion of IL-6 and IL-10 by AM were increased dramatically. On the contrary, the secretion of IL-6 by PM was declined from the 1st day to the 4th day, then increased even over that of the normal control group on the 7th day. However, the secretion of IL-10 by PM was significantly elevated on the 1st day after trauma hemorrhage, peaking on the 4th day, and only slight lowering was found on the 7th day. The secretion of IL-6 by Mo was declined gradually all the time, reaching the lowest point on the 7th day. On the contrary, the secretion of IL-10 by Mo was increasing, reaching its peak on the 7th day.
  
  
  CONCLUSIONS
  The heterogeneity of secretion ability of Mo/macrophage obtained from different anatomical regions is present under normal condition, and is more obvious following a severe injury. This change may play an important role in the immune dysfunction and the development of complications after trauma.
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Department One, Research Institute of Surgery, Daping Hospital, The Third Military Medical University, Chongqing 400042, China.
authors.forename: Hua-hua
authors.initials: HH
authors.lastname: Yin
authors.forename: Hao
authors.initials: H
authors.lastname: Xia
authors.forename: Jing-ci
authors.initials: JC
authors.lastname: Zhu
authors.forename: Chang-guo
authors.initials: CG
authors.lastname: Gu
authors.forename: Fa-liang
authors.initials: FL
authors.lastname: Xu
authors.forename: Lei
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000503:physiopathology*; D013997:Time Factors
chemical_list: D015850:Interleukin-6; D016753:Interleukin-10