title: Peripheral blood B-cell death compensates for excessive proliferation in lymphoid tissues and maintains homeostasis in bovine leukemia virus-infected sheep.
abstract: The size of a lymphocyte population is primarily determined by a dynamic equilibrium between cell proliferation and death. Hence, lymphocyte recirculation between the peripheral blood and lymphoid tissues is a key determinant in the maintenance of cell homeostasis. Insights into these mechanisms can be gathered from large-animal models, where lymphatic cannulation from individual lymph nodes is possible. In this study, we assessed in vivo lymphocyte trafficking in bovine leukemia virus (BLV)-infected sheep. With a carboxyfluorescein diacetate succinimidyl ester labeling technique, we demonstrate that the dynamics of lymphocyte recirculation is unaltered but that accelerated proliferation in the lymphoid tissues is compensated for by increased death in the peripheral blood cell population. Lymphocyte homeostasis is thus maintained by biphasic kinetics in two distinct tissues, emphasizing a very dynamic process during BLV infection.
authors.affiliation: Molecular and Cellular Biology, FNRS-FUSAG, Gembloux, Belgium.
authors.forename: Christophe
authors.initials: C
authors.lastname: Debacq
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gillet
authors.forename: Becca
authors.initials: B
authors.lastname: Asquith
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Sanchez-Alcaraz
authors.forename: Arnaud
authors.initials: A
authors.lastname: Florins
authors.forename: Mathieu
authors.initials: M
authors.lastname: Boxus
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schwartz-Cornil
authors.forename: Michel
authors.initials: M
authors.lastname: Bonneau
authors.forename: Geneviève
authors.initials: G
authors.lastname: Jean
authors.forename: Pierre
authors.initials: P
authors.lastname: Kerkhofs
authors.forename: Jack
authors.initials: J
authors.lastname: Hay
authors.forename: André
authors.initials: A
authors.lastname: Théwis
authors.forename: Richard
authors.initials: R
authors.lastname: Kettmann
authors.forename: Luc
authors.initials: L
authors.lastname: Willems
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D039481:CD11b Antigen / Q000378:metabolism; D016923:Cell Death; D002465:Cell Movement; D049109:Cell Proliferation; D006800:Deltaretrovirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D005452:Fluoresceins; D006706:Homeostasis; D007700:Kinetics; D001909:Leukemia Virus, Bovine / Q000276:immunology / Q000502:physiology*; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology* / Q000473:pathology / Q000821:virology*; D012756:Sheep / Q000276:immunology / Q000821:virology; D012757:Sheep Diseases / Q000276:immunology / Q000821:virology; D013388:Succinimides
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D039481:CD11b Antigen; D005452:Fluoresceins; D013388:Succinimides