Rapid ecotoxicological bioassay using delayed fluorescence in the green alga Pseudokirchneriella subcapitata.
A rapid and simple ecotoxicological bioassay allows quick estimation of the effects of Simazine (CAT) or 3,5-dichlorophenol (3,5-DCP) on the growth of the green alga Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum). The effects of a 15-min exposure to CAT or 3,5-DCP on delayed fluorescence (DF) in P. subcapitata were compared to the results of standard growth inhibition tests involving 72h of exposure to these chemicals at the same concentrations. Integrated DF intensity in the time period from 0.6 to 50s was found to correlate with algal growth inhibition as measured by the standard tests. In addition, the behaviour of DF in this time period exhibited characteristic kinetics indicative of the chemical being tested.
Katsumata
Masakazu
M
Central Research Laboratory, Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamamatsu City, Shizuoka Prefecture 434-8601, Japan. katumata@crl.hpk.co.jp <katumata@crl.hpk.co.jp>
Koike
Takashi
T
Nishikawa
Masataka
M
Kazumura
Kimiko
K
Tsuchiya
Hiroshi
H
D001681:Biological Assay / Q000379:methods; D002733:Chlorophenols / Q000032:analysis*; D000460:Chlorophyta / Q000737:chemistry / Q000254:growth & development*; D005453:Fluorescence; D006540:Herbicides / Q000032:analysis*; D007700:Kinetics; D012839:Simazine / Q000032:analysis*; D013997:Time Factors
D002733:Chlorophenols; D006540:Herbicides; C116190:3,5-dichlorophenol; D012839:Simazine