Topology characterization by MALDI-ToF-MS of enzymatically synthesized poly(lactide-co-glycolide).
Lipase catalyzed copolymerization of the monomers lactide and glycolide by Pseudomonas cepacia employing a molar ratio of 80L/20G has been studied. The copolymers were characterized by MALDI-ToF-MS, DSC, SEC and NMR. MALDI-ToF-MS has successfully been used not only to determine end groups and chemical composition but even the microstructure of the copolymers. We demonstrated that for this lipase catalyzed copolymerization, the main product of the reaction at 100 degrees C was linear homopolymer of lactide while at 130 degrees C the main product was cyclic random copolymer.
Huijser
Saskia
S
Laboratory of Polymer Chemistry, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
Staal
Bastiaan B P
BB
Huang
Juan
J
Duchateau
Robbert
R
Koning
Cor E
CE
D001672:Biocompatible Materials / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods; D016956:Burkholderia cepacia / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D002384:Catalysis; D002412:Cations; D008049:Lipase / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D011098:Polyglactin 910 / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013696:Temperature
D001672:Biocompatible Materials; D002412:Cations; D046911:Macromolecular Substances; D011108:Polymers; D011098:Polyglactin 910; D008049:Lipase