title: Identification and characterization of the chemotactic transducer in Pseudomonas aeruginosa PAO1 for positive chemotaxis to trichloroethylene.
abstract: Pseudomonas aeruginosa PAO1 is repelled by trichloroethylene (TCE), and the methyl-accepting chemotaxis proteins PctA, PctB, and PctC serve as the major chemoreceptors for negative chemotaxis to TCE. In this study, we found that the pctABC triple mutant of P. aeruginosa PAO1 was attracted by TCE. Chemotaxis assays of a set of mutants containing deletions in 26 potential mcp genes revealed that mcpA (PA0180) is the chemoreceptor for positive chemotaxis to TCE. McpA also detects tetrachloroethylene and dichloroethylene isomers as attractants.
authors.affiliation: Department of Molecular Biotechnology, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
authors.forename: Hye-Eun
authors.initials: HE
authors.lastname: Kim
authors.forename: Maiko
authors.initials: M
authors.lastname: Shitashiro
authors.forename: Akio
authors.initials: A
authors.lastname: Kuroda
authors.forename: Noboru
authors.initials: N
authors.lastname: Takiguchi
authors.forename: Hisao
authors.initials: H
authors.lastname: Ohtake
authors.forename: Junichi
authors.initials: J
authors.lastname: Kato
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002633:Chemotaxis / Q000235:genetics*; D017353:Gene Deletion; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000502:physiology*; D014241:Trichloroethylene / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; C110124:PctA protein, Pseudomonas aeruginosa; C110122:PctB protein, Pseudomonas aeruginosa; C110123:PctC protein, Pseudomonas aeruginosa; D014241:Trichloroethylene