title: Peanut allergen exposure through saliva: assessment and interventions to reduce exposure.
abstract: BACKGROUND
  Exposure to food allergens through saliva (kissing, utensils) can cause local and systemic allergic reactions.
  
  
  OBJECTIVE
  To determine the time course of peanut allergen (Ara h 1) persistence in saliva after ingestion of peanut butter and to evaluate mouth cleansing interventions to reduce salivary peanut allergen.
  
  
  METHODS
  Thirty-eight individuals ingested 2 tablespoons of peanut butter, and saliva was collected at various time points. At another time, samples were collected after 5 interventions (brushing teeth, brushing and rinsing, rinsing, waiting then brushing, waiting then chewing gum). Detection of Ara h 1 was performed by a monoclonal-based ELISA (detection limit, 15-20 ng/mL).
  
  
  RESULTS
  Salivary Ara h 1 varied considerably immediately after ingestion, but included levels expected to invoke reactions (as much as 40 microg/mL). Most (87%) subjects with detectable peanut after a meal had undetectable levels by 1 hour with no interventions. None had detectable levels several hours later after a peanut-free lunch. This result indicates (95% confidence) that 90% would have undetectable Ara h 1 in saliva under these circumstances. All of the interventions reduced salivary Ara h 1, in some cases by >95%, but Ara h 1 remained detectable in approximately 40% of samples (though typically below thresholds reported to induce reactions).
  
  
  CONCLUSIONS
  Patients with peanut allergy require counseling regarding risks of kissing or sharing utensils, even if partners have brushed teeth or chewed gum. Advice to reduce risks, though not as ideal as total avoidance, includes waiting a few hours plus eating a peanut-free meal.
  
  
  CONCLUSIONS
  Waiting several hours and ingesting a peanut-free meal were more effective at reducing salivary peanut protein concentration than simple, immediate interventions.
authors.affiliation: Elliot and Roslyn Jaffe Food Allergy Institute, Division of Allergy and Immunology, Department of Pediatrics, Mount Sinai School of Medicine, New York, NY, USA. jennifer.maloney@mssm.edu
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Maloney
authors.forename: Martin D
authors.initials: MD
authors.lastname: Chapman
authors.forename: Scott H
authors.initials: SH
authors.lastname: Sicherer
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant; D010367:Arachis / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins; D021183:Peanut Hypersensitivity / Q000175:diagnosis* / Q000276:immunology* / Q000517:prevention & control; D010865:Pilot Projects; D010940:Plant Proteins / Q000276:immunology*; D012463:Saliva / Q000276:immunology*; D013997:Time Factors
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; D006023:Glycoproteins; D008565:Membrane Proteins; D010940:Plant Proteins