Does overfeeding enhance genotype effects on liver ability for lipogenesis and lipid secretion in ducks?
We evaluated the effects of genotype (Muscovy, Pekin and their crossbreed hinny and mule ducks) and feeding levels (overfeeding between 12 and 14 weeks of age vs ad libitum feeding) on liver ability for lipogenesis and lipid secretion in ducks. Samples of liver and blood were collected at 14 weeks of age from 8 birds per group. Plasma levels of insulin was considerably increased in overfed ducks (1.9-fold), stimulating the hepatic activity of the main enzymes involved in lipogenesis from glucose (glucokinase, GK, glucose-6-phosphate dehydrogenase, G6PDH, malic enzyme, ME, acetyl CoA carboxylase, ACX), while cytochrome-c oxidase (COX) activity, indicating overall oxidation ability of energy-yielding substrates, remained unchanged. Plasma levels of triglycerides, phospholipids and total cholesterol were therefore increased (1.9, 3.7, 1.6 and 1.6-fold, respectively). Glycaemia also significantly increased (+8%). Pekin ducks exhibited higher levels of GK and G6PDH activity in the liver than Muscovy ducks, suggesting a greater ability to use glucose consistent with their lower glycaemia. Muscovy ducks had greater ACX activity, suggesting greater ability to synthesise lipids. However, plasma lipid levels were much higher in Pekin ducks than in Muscovy ducks, suggesting a greater ability to export lipids from the liver. Values for the different criteria measured in this study were intermediate or similar in hinny and mule ducks to those of parental species. The high values for GK, G6PDH, ME and ACX activity in hybrid ducks enabled them to produce heavy fatty livers with the same chemical and lipid composition as Muscovy ducks and characterised by high amounts of triglycerides (around 96% of total lipids), and saturated and mono-unsaturated fatty acids.
Chartrin
Pascal
P
Station de Recherches Avicoles, INRA Tours, 37380 Nouzilly, France. chartrin@tours.inra.fr
Bernadet
Marie-Dominique
MD
Guy
Gérard
G
Mourot
Jacques
J
Hocquette
Jean-François
JF
Rideau
Nicole
N
Duclos
Michel Jacques
MJ
Baéza
Elisabeth
E
D000821:Animal Feed; D000818:Animals; D001786:Blood Glucose; D004372:Ducks / Q000235:genetics* / Q000378:metabolism*; D004435:Eating; D005260:Female; D005838:Genotype; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000097:blood; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D050155:Lipogenesis; D008099:Liver / Q000378:metabolism*; D008297:Male
D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D005947:Glucose