A self-contained polymeric 2-DE chip system for rapid and easy analysis.
We developed a polymeric 2-DE chip system. The chip consisted of an IEF region, an SDS-PAGE region, a valveless connection port, and a sample introduction port. A "junction structure" as a valveless connection port, which allowed separating and connecting the first- and second-dimensional gels, was fabricated between their regions. A "solution inlet" as a sample introduction port was fabricated to perform the liquid and sample introductions without solution leakage. Simultaneous sample monitoring was performed using the on-chip detection system. The performances of the system were demonstrated using commercially available proteins as a standard specimen and tissue-extracted proteins as the real samples. All procedures were employed without any movement of relocation part. This new 2-D separation system realized improved labor-intensive operations and a reduced experimental time.
Usui
Keisuke
K
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan.
Hiratsuka
Atsunori
A
Shiseki
Kisho
K
Maruo
Yuji
Y
Matsushima
Toshiyuki
T
Takahashi
Katsuyoshi
K
Unuma
Yutaka
Y
Sakairi
Koji
K
Namatame
Ichiji
I
Ogawa
Yasuhiro
Y
Yokoyama
Kenji
K
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
D011108:Polymers; D011506:Proteins