Electrocatalytic reduction of hydrogen peroxide on modified graphite electrodes: application to the development of glucose biosensors.
The electrocatalytic activities of a series of compact graphites modified with microquantities of platinum metals (Pd or Pt+Pd) towards the electrochemical reduction of hydrogen peroxide were characterised. Operational parameters such as the optimal working potential, the influence of temperature and the resulting electrode characteristics were examined. The benefits of using graphite modified with Pt+Pd (mixture ratio 30%:70%) as the basic transducer in a glucose biosensor with improved sensitivity were demonstrated. It was proven that, under the working conditions chosen, the selected electrode (whether bare or covered with an enzyme layer) did not respond to any glutathione, uric acid or ascorbic acid (which all normally occur in biological fluids) present.
Dodevska
T
T
Department of Inorganic and Physical Chemistry, University of Food Technology, 26 Maritsa blvd., Plovdiv 4002, Bulgaria.
Horozova
E
E
Dimcheva
N
N
D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D010084:Oxidation-Reduction; D010165:Palladium / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013696:Temperature
D004800:Enzymes, Immobilized; D010984:Platinum; D010165:Palladium; D006108:Graphite; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase