title: [Molecular cloning and homologous modeling of Wulong goose MHC class I gene].
abstract: Major histocompatibility complex (MHC) was correlated with immune response for extra-antigen. MHC sequences of chicken, other birds, crawl species and mammalian were derived from GenBank/DDBJ/EMBL and analyzed by alignment, and then primers were designed. By means of LA-PCR method, MHC Classgene was cloned from Wulong goose genomic DNA and total RNA, and the structure of MHC Classgenomic DNA was analyzed using bioinformation methods. The results showed that the genomic DNA, which consisted of 8 extrons and 7 introns, had 64.1% amino acids homologous to chicken and 42.9% amino acids to human. The molecular phylogenetic tree further revealed evolutionary relationship between Wulong goose and other animals such as chicken, other birds, crawl species, mammalian and human. The results of homologous modeling showed that Wulong Goose MHC Classgene was formed by amidocyanogen (N) terminal structure region and carboxyl (C) terminal structure region.
authors.affiliation: Institute of High Quality Waterfowl, Laiyang Agricultural University, Qingdao, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Jia
authors.forename: Bao-Wei
authors.initials: BW
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Ming-Ai
authors.initials: MA
authors.lastname: Zhang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Wu
authors.forename: Guang-Lei
authors.initials: GL
authors.lastname: Liu
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Yang
authors.forename: Fang-Yu
authors.initials: FY
authors.lastname: Long
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D019295:Computational Biology; D005091:Exons / Q000235:genetics; D005777:Geese / Q000235:genetics*; D005805:Genes, MHC Class I / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics; D008958:Models, Molecular; D010802:Phylogeny; D017433:Protein Structure, Secondary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D015395:Histocompatibility Antigens Class I