Differential gene expression by Paracoccidioides brasiliensis in host interaction conditions: representational difference analysis identifies candidate genes associated with fungal pathogenesis.
Paracoccidioides brasiliensis causes infection by the host inhalation of airborne propagules of the mycelia phase of the fungus. These particles reach the lungs, and disseminate to virtually all organs. Here we describe the identification of differentially expressed genes in studies of host-fungus interaction. We analyzed two cDNA populations of P. brasiliensis, one obtained from infected animals and the other an admixture of fungus and human blood thus mimicking the hematologic events of the fungal dissemination. Our analysis identified transcripts differentially expressed. Genes related to iron acquisition, melanin synthesis and cell defense were specially upregulated in the mouse model of infection. The upregulated transcripts of yeast cells during incubation with human blood were those predominantly related to cell wall remodeling/synthesis. The expression pattern of genes was independently confirmed in host conditions, revealing their potential role in the infection process. This work can facilitate functional studies of novel regulated genes that may be important for the survival and growth strategies of P. brasiliensis in humans.
Bailão
Alexandre Melo
AM
Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001-970 Goiânia, Goiás, Brazil.
Schrank
Augusto
A
Borges
Clayton Luiz
CL
Dutra
Valéria
V
Walquíria Inês Molinari-Madlum
Eugênia Emília
EE
Soares Felipe
Maria Sueli
MS
Soares Mendes-Giannini
Maria José
MJ
Martins
Wellington Santos
WS
Pereira
Maristela
M
Maria de Almeida Soares
Célia
C
D000222:Adaptation, Physiological / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D016469:Fungemia / Q000382:microbiology; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005800:Genes, Fungal; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D010228:Paracoccidioides / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D010229:Paracoccidioidomycosis / Q000382:microbiology*; D012331:RNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics
D018076:DNA, Complementary; D012331:RNA, Fungal; D012333:RNA, Messenger