title: Alterations in the mitochondrial proteome of adriamycin resistant MCF-7 breast cancer cells.
abstract: A 2D-gel based comparative proteomic analysis has been conducted of changes in mitochondrial protein abundances in MCF-7 human breast cancer cells selected for resistance to Adriamycin accompanied by verapamil. We identified and compared 156 unique proteins from 184 spots. Eleven mitochondrial proteins were found with abundances altered more than 2-fold. Transcription was evaluated for two of these, using quantitative RT-PCR. Implications of the changes are considered with respect to drug resistance.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland, USA.
authors.forename: Rachael
authors.initials: R
authors.lastname: Strong
authors.forename: Takeo
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Douglas
authors.initials: D
authors.lastname: Ross
authors.forename: Catherine
authors.initials: C
authors.lastname: Fenselau
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D017931:DNA Primers; D004317:Doxorubicin; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D013058:Mass Spectrometry; D008928:Mitochondria / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D011506:Proteins; D004317:Doxorubicin