title: An integrated systems approach for understanding cellular responses to gamma radiation.
abstract: Cellular response to stress entails complex mRNA and protein abundance changes, which translate into physiological adjustments to maintain homeostasis as well as to repair and minimize damage to cellular components. We have characterized the response of the halophilic archaeon Halobacterium salinarum NRC-1 to (60)Co ionizing gamma radiation in an effort to understand the correlation between genetic information processing and physiological change. The physiological response model we have constructed is based on integrated analysis of temporal changes in global mRNA and protein abundance along with protein-DNA interactions and evolutionarily conserved functional associations. This systems view reveals cooperation among several cellular processes including DNA repair, increased protein turnover, apparent shifts in metabolism to favor nucleotide biosynthesis and an overall effort to repair oxidative damage. Further, we demonstrate the importance of time dimension while correlating mRNA and protein levels and suggest that steady-state comparisons may be misleading while assessing dynamics of genetic information processing across transcription and translation.
authors.affiliation: Institute for Systems Biology, Seattle, WA 98103-8904, USA.
authors.forename: Kenia
authors.initials: K
authors.lastname: Whitehead
authors.forename: Adrienne
authors.initials: A
authors.lastname: Kish
authors.forename: Min
authors.initials: M
authors.lastname: Pan
authors.forename: Amardeep
authors.initials: A
authors.lastname: Kaur
authors.forename: David J
authors.initials: DJ
authors.lastname: Reiss
authors.forename: Nichole
authors.initials: N
authors.lastname: King
authors.forename: Laura
authors.initials: L
authors.lastname: Hohmann
authors.forename: Jocelyne
authors.initials: J
authors.lastname: DiRuggiero
authors.forename: Nitin S
authors.initials: NS
authors.lastname: Baliga
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D005720:Gamma Rays; D019848:Gene Expression Regulation, Archaeal / Q000528:radiation effects; D006217:Halobacterium / Q000235:genetics / Q000502:physiology / Q000528:radiation effects*; D008954:Models, Biological; D011485:Protein Binding / Q000528:radiation effects; D014176:Protein Biosynthesis / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000528:radiation effects
chemical_list: D019843:Archaeal Proteins; D012333:RNA, Messenger