"Click" synthesis of small molecule probes for activity-based fingerprinting of matrix metalloproteases.
By using "Click Chemistry", we achieved the facile synthesis of various affinity-based hydroxamate probes that enable generation of activity-based fingerprints of a variety of metalloproteases, including matrix metalloproteases (MMPs), in proteomics experiments.
Wang
Jun
J
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543.
Uttamchandani
Mahesh
M
Li
Junqi
J
Hu
Mingyu
M
Yao
Shao Q
SQ
D001665:Binding Sites; D003878:Dermatoglyphics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006877:Hydroxamic Acids / Q000138:chemical synthesis* / Q000494:pharmacology; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000494:pharmacology; D015394:Molecular Structure; D040901:Proteomics
D004791:Enzyme Inhibitors; D006877:Hydroxamic Acids; D061965:Matrix Metalloproteinase Inhibitors; D015335:Molecular Probes; D020782:Matrix Metalloproteinases