title: Dietary fish oil reduces the occurrence of early afterdepolarizations in pig ventricular myocytes.
abstract: Fish oil reduces sudden cardiac death in post myocardial infarction patients. Life-threatening arrhythmias in heart failure are associated with repolarization abnormalities leading to EAD(1) formation. We examined the effects of incorporated fish oil omega3-PUFAs(2) on EAD formation in pig myocytes. Pigs were fed a diet rich in fish oil or sunflower oil (control) for 8 weeks. Myocytes were isolated by enzymatic dissociation and patch-clamped. Susceptibility to EAD formation was tested using E4031 (5 microM), a blocker of I(Kr). The fish oil diet in pigs resulted in increased incorporation of omega3-PUFAs in the sarcolemma of the myocytes compared to the control diet and caused a reduced occurrence of E4031-induced EADs in pig myocytes. A shorter action potential, a reduced action potential prolongation in response to E-4031 and a reduced reactivation of I(Ca,L) by omega3-PUFAs may explain the observed reduction in EADs. A diet rich in fish oil protects against EAD formation.
authors.affiliation: Department of Clinical and Experimental Cardiology, Experimental and Molecular Cardiology Groups, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Hester M
authors.initials: HM
authors.lastname: Den Ruijter
authors.forename: Arie O
authors.initials: AO
authors.lastname: Verkerk
authors.forename: Géza
authors.initials: G
authors.lastname: Berecki
authors.forename: Diane
authors.initials: D
authors.lastname: Bakker
authors.forename: Antoni C G
authors.initials: AC
authors.lastname: van Ginneken
authors.forename: Ruben
authors.initials: R
authors.lastname: Coronel
mesh_terms: D000200:Action Potentials; D000818:Animals; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology*; D006352:Heart Ventricles / Q000166:cytology / Q000187:drug effects*; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects*; D013552:Swine; D016276:Ventricular Function
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils