title: Direct nitric oxide measurement in human spermatozoa: flow cytometric analysis using the fluorescent probe, diaminofluorescein.
abstract: We have demonstrated that intracellular nitric oxide (NO) can be detected and measured directly in human spermatozoa by flow cytometry using the fluorescent probe 4,5-diaminofluorescein-2/diacetate (10 microM). This method can measure both exogenously added as well as endogenously generated intracellular NO.
authors.affiliation: Department of Medical Physiology and Biochemistry, University of Stellenbosch, Tygerberg, South Africa.
authors.forename: Fanuel
authors.initials: F
authors.lastname: Lampiao
authors.forename: Hans
authors.initials: H
authors.lastname: Strijdom
authors.forename: Stefan S
authors.initials: SS
authors.lastname: du Plessis
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D019793:Fluorescein; D005456:Fluorescent Dyes; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D009569:Nitric Oxide / Q000032:analysis*; D013094:Spermatozoa / Q000737:chemistry*
chemical_list: C414689:4,5-diaminofluorescein diacetate; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009569:Nitric Oxide; D019793:Fluorescein