title: Resistance of herpes simplex virus type 1 to acyclovir: thymidine kinase gene mutagenesis study.
abstract: By site-directed mutagenesis, we investigate the role of six mutations of herpes simplex virus type 1 thymidine kinase (TK) gene in the acquisition of resistance to acyclovir (ACV). TK activity was not impaired by substitutions located at codons 17, 161 and 374 and these mutations were thus related to TK gene polymorphism. Mutations His105Pro, Leu364Pro and Asp162Ala lead to the loss of TK activity that could result in ACV-resistance.
authors.affiliation: Laboratoire de Virologie Est, Hospices Civils de Lyon, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France. e.frobert@wanadoo.fr
authors.forename: Emilie
authors.initials: E
authors.lastname: Frobert
authors.forename: Tadamasa
authors.initials: T
authors.lastname: Ooka
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Cortay
authors.forename: Bruno
authors.initials: B
authors.lastname: Lina
authors.forename: Danielle
authors.initials: D
authors.lastname: Thouvenot
authors.forename: Florence
authors.initials: F
authors.lastname: Morfin
mesh_terms: D000212:Acyclovir / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D024882:Drug Resistance, Viral / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D006801:Humans; D016297:Mutagenesis, Site-Directed / Q000379:methods; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D013937:Thymidine Kinase; D000212:Acyclovir