How to assess and study cell death in Dictyostelium discoideum.
In this chapter, we describe how to conveniently demonstrate, assess, and study cell death in Dictyostelium through simple cell culture, clonogenic tests, and photonic (with the help of staining techniques) and electronic microscopy. Cell death can be convniently generated using minor modifications of the monolayer technique of Rob Kay et al., and either wild-type HMX44A Dictyostelium cells or the corresponding atg1- autophagy gene mutant cells. Methods to follow cell death qualitatively and quantitatively facilitate detailed studies of vacuolar death in wild-type cells and of nonvacuolar, "condensed" death in atg1- mutant cells.
Kosta
Artemis
A
Centre d'Immunologie INSERM-CNRS-Univ. Medit. de Marseille-Luminy, France.
Laporte
Catherine
C
Lam
David
D
Tresse
Emilie
E
Luciani
Marie-Françoise
MF
Golstein
Pierre
P
D000818:Animals; D001343:Autophagy; D016923:Cell Death; D003114:Colony-Forming Units Assay; D004023:Dictyostelium / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D005434:Flow Cytometry