Polymorphism of gp15/400 allergen gene of W. bancrofti from different regions of India endemic for lymphatic filariasis.
Nematode polyprotein allergens (NPA) are lipid binding/transport molecules that elicit elevated levels of IgE response in the infected host, leading to Th2 type of immune response. They also transport arachidonic acid and its metabolites that are known to be involved in the action of antifilarial drug, Diethylcarbamazine and hence are of great significance for the control of lymphatic filariasis. We investigated the polymorphism of gp15/400 polyprotein of 35 isolates of lymphatic filarial parasite Wuchereria bancrofti collected from different geographic locations of India. The repeat sub-unit of the gene was found to be highly conserved in all the isolates with only two nucleotide synonymous changes at positions 286 (A-G) and 337 (C-T). Since this molecule is highly conserved and has multifarious roles in the survival and pathogenesis of the parasite it has good potential as a target for drug, immunodulation tool and immunotherapy development.
Hoti
S L
SL
Molecular Biology and Bioinformatics Unit, Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India. slhoti@yahoo.com
Thangadurai
R
R
Patra
K P
KP
Das
P K
PK
D000485:Allergens / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D004605:Elephantiasis, Filarial / Q000453:epidemiology / Q000276:immunology / Q000469:parasitology*; D019353:Endemic Diseases; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics*; D007194:India / Q000453:epidemiology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D014958:Wuchereria bancrofti / Q000235:genetics* / Q000276:immunology
D000485:Allergens; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C520090:GP15-400 protein, Wuchereria bancrofti; D015801:Helminth Proteins; D008562:Membrane Glycoproteins