Effect of administration of prostaglandin F2alpha or presence of an estrous teaser bitch on characteristics of the canine ejaculate.
Semen was collected from eight dogs after SC administration of 0.1mg/kg PGF2alpha or 0.6 mL 0.9% NaCl solution 15 min prior to collection in the presence or absence of an estrous teaser bitch (switchback design; all dogs given all four treatments in random sequence). There were more spermatozoa (P=0.02) in ejaculates collected after administration of PGF2alpha in the presence of an estrous teaser bitch ((852+/-736)x10(6), mean+/-S.D.) than in ejaculates collected in saline-treated dogs in the absence of a teaser bitch ((371+/-620)x10(6)). However, the number of spermatozoa in the ejaculate of dogs given PGF2alpha in the absence of a teaser bitch and in dogs given saline in the presence of a teaser bitch ((556+/-494 and 600+/-622)x10(6), respectively) were not significantly different from each other or from the other two groups. The percentage of morphologically normal spermatozoa did not vary by treatment (P=0.51). In conclusion, treatment with PGF2alpha and presence of a teaser bitch had an additive effect on the number of spermatozoa. This, coupled with relatively minor side-effects, suggests this is a useful technique to increase number of spermatozoa in a single canine ejaculate.
Kustritz
Margaret V Root
MV
University of Minnesota, College of Veterinary Medicine, 1352 Boyd Avenue, St. Paul, MN 55108, USA. rootk001@umn.edu
Hess
Milan
M
D000818:Animals; D018592:Cross-Over Studies; D015237:Dinoprost / Q000494:pharmacology*; D004285:Dogs / Q000502:physiology*; D004542:Ejaculation / Q000187:drug effects / Q000502:physiology*; D004971:Estrus / Q000502:physiology*; D005260:Female; D007279:Injections, Subcutaneous / Q000662:veterinary; D008297:Male; D012661:Semen / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013076:Sperm Count / Q000662:veterinary; D013094:Spermatozoa / Q000502:physiology*
D015237:Dinoprost