Identification of a newly spliced exon in the mouse Ilf3 gene generating two long and short isoforms of Ilf3 and NF90.
The mammalian IlF3 and NF90 proteins, involved in several cellular functions, have common N-terminal and central sequences and specific C-terminal regions. These proteins exhibit a large heterogeneity generated by posttranscriptional and posttranslational modifications. Part of their polymorphism is due to the alternative splicing of exon 3 located just downstream of the translation initiation codon. This 39-nucleotide-long exon, not described so far, codes for an N-terminal sequence of 13 residues (ALYHHHFITRRRR) also present in rat and human IlF3 or NF90. Four mRNAs are expressed in mouse brain, two for Ilf3 and two for NF90, differing in their 3' sequence to generate the specific Ilf3 and NF90 C-terminal domains and in the presence or the absence of exon 3 to generate long and short isoforms of both proteins. By RT-PCR, no other variants were found. Combining our results and GenBank sequences, we determined the exon-intron organization of the entire mouse Ilf3 gene divided into 22 exons.
Viranaicken
Wildriss
W
Laboratoire de Biochimie Cellulaire, UMR 7098 CNRS, Université Pierre et Marie Curie, 9 Quai Saint-Bernard, Case 265, 75252 Paris Cedex 05, France.
Gasmi
Laïla
L
Chauvin
Céline
C
Denoulet
Philippe
P
Larcher
Jean-Christophe
JC
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D005091:Exons; D023281:Genomics; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D050779:Nuclear Factor 90 Proteins / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
C494015:Ilf3 protein, mouse; D050779:Nuclear Factor 90 Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D004247:DNA