title: Molecular epidemiology of multidrug-resistant Mycobacterium bovis isolates with the same spoligotyping profile as isolates from animals.
abstract: PCR-based characterization techniques have been adopted in most laboratories for Mycobacterium bovis typing. We report a molecular characterization of human multidrug-resistant M. bovis isolates and three bovine isolates that share the spoligotyping profile. The analysis of the direct repeat region showed that both groups differed in the presence of spacers not included in the current membrane. They were also distinguished by two out of the nine mycobacterial interspersed repetitive unit variable-number tandem repeat loci tested, indicating that the human infection was not acquired from the cattle from which isolates were obtained. These results highlight that a combination of techniques is required for appropriate discrimination, even for those spoligotypes that have a low frequency.
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Romero
authors.forename: Alicia
authors.initials: A
authors.lastname: Aranaz
authors.forename: Lucía
authors.initials: L
authors.lastname: de Juan
authors.forename: Julio
authors.initials: J
authors.lastname: Alvarez
authors.forename: Javier
authors.initials: J
authors.lastname: Bezos
authors.forename: Ana
authors.initials: A
authors.lastname: Mateos
authors.forename: Enrique
authors.initials: E
authors.lastname: Gómez-Mampaso
authors.forename: Lucas
authors.initials: L
authors.lastname: Domínguez
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D002417:Cattle; D006801:Humans; D008826:Microbial Sensitivity Tests; D018598:Minisatellite Repeats / Q000235:genetics; D017720:Molecular Epidemiology; D009163:Mycobacterium bovis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000032:analysis*; D016133:Polymerase Chain Reaction; D014380:Tuberculosis, Bovine / Q000453:epidemiology* / Q000382:microbiology; D018088:Tuberculosis, Multidrug-Resistant / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D009841:Oligonucleotides