title: [Quercetin inhibits growth and induces apoptosis of human gastric carcinoma cells].
abstract: OBJECTIVE
  To study the effect of quercetin on the growth and apoptosis of human gastric carcinoma cell line MGC-803.
  
  
  METHODS
  The measurement of inhibitory rate and apoptotic index(AI) of quercetin were done by MTT assay and TUNEL assay. The positive expression rate of P53, C-myc and P16 were detected by immunocytochemical staining.
  
  
  RESULTS
  Quercetin at concentrations ranging from 40 mumol/L to 100 mumol/L significantly inhibited the proliferation of MGC-803 cells in a dose- and time-dependent manner (P<0.01). TUNEL assay indicated that the number of apoptotic cells in quercetin-treated group was greater than that in the control group (P<0.01). Expression of P53 and C-myc protein decreased following quercetin induction in a dose-dependent manner, whereas P16 expression increased significantly compared with that of the control group (P<0.01).
  
  
  CONCLUSIONS
  Quercetin can inhibit the growth and induce apoptosis of MGC-803 cells in a dose- and time-dependent manner. Its mechanisms may be relevant to the down-regulation of P53 and C-myc protein expression as well as up-regulation of P16 expression.
authors.affiliation: Institute of Cellular and Molecular Biology, College of Medical Science, Wuhan University of Science and Technology, Wuhan 430080, China. hly_wust@yahoo.com.cn
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Liang-miao
authors.initials: LM
authors.lastname: Guo
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Xue-hua
authors.initials: XH
authors.lastname: Zhao
authors.forename: Cai-lian
authors.initials: CL
authors.lastname: Cheng
authors.forename: Mian-yun
authors.initials: MY
authors.lastname: Wu
authors.forename: Li-ya
authors.initials: LY
authors.lastname: He
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019941:Cyclin-Dependent Kinase Inhibitor p16; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C000614131:CDKN2A protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; C489427:MYC protein, human; D009363:Neoplasm Proteins; D016271:Proto-Oncogene Proteins c-myc; D016159:Tumor Suppressor Protein p53; D011794:Quercetin