title: Correlation between initial chromatid damage and survival of various cell lines exposed to heavy charged particles.
abstract: The biophysical characteristics of heavy ions make them a rational source of radiation for use in radiotherapy of malignant tumours. Prior to radiotherapy treatment, a therapeutic regimen must be precisely defined, and during this stage information on individual patient radiosensitivity would be of very great medical value. There are various methods to predict radiosensitivity, but some shortfalls are difficult to avoid. The present study investigated the induction of chromatid breaks in five different cell lines, including one normal liver cell line (L02), exposed to carbon ions accelerated by the heavy ion research facility in Lanzhou (HIRFL), using chemically induced premature chromosome condensation (PCC). Previous studies have reported the number of chromatid breaks to be linearly related to the radiation dose, but the relationship between cell survival and chromatid breaks is not clear. The major result of the present study is that cellular radiosensitivity, as measured by D0, is linearly correlated with the frequency of chromatid breaks per Gy in these five cell lines. We propose that PCC may be applied to predict radiosensitivity of tumour cells exposed to heavy ions.
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, 730000, China. yangjs@impcas.ac.cn
authors.forename: Yang
authors.initials: Y
authors.lastname: Jianshe
authors.forename: Jing
authors.initials: J
authors.lastname: Xigang
authors.forename: Li
authors.initials: L
authors.lastname: Wenjian
authors.forename: Wang
authors.initials: W
authors.lastname: Zhuanzi
authors.forename: Zhou
authors.initials: Z
authors.lastname: Guangming
authors.forename: Wang
authors.initials: W
authors.lastname: Jufang
authors.forename: Dang
authors.initials: D
authors.lastname: Bingrong
authors.forename: Gao
authors.initials: G
authors.lastname: Qingxiang
authors.forename: Walsh
authors.initials: W
authors.lastname: Linda
mesh_terms: D002244:Carbon / Q000737:chemistry; D002460:Cell Line; D002842:Chromatids; D019457:Chromosome Breakage / Q000528:radiation effects*; D020732:Cytogenetic Analysis; D006801:Humans; D011836:Radiation Tolerance; D011878:Radiotherapy / Q000009:adverse effects*
chemical_list: D002244:Carbon