Calves born from anestrus yaks (Poephagus grunniens L.) subjected to Ovsynch and superovulation treatment.
An attempt was made to induce estrus and ovulation in eight anestrus yaks by use of the Ovsynch protocol. Six out of eight yaks were successfully induced into estrus, and ovulation occurred in all the responding yaks 1-2 days after the second GnRH administration. Out of the six animals that responded to the treatment, two mated naturally with yak bulls, and calves were obtained from them. The other four animals were further administered a superovulatory regimen of Folltropin (FSH-P). Following Folltropin and Ilerin (a PGF(2alpha) analog) treatment, the animals were subjected to natural insemination. Only one animal in which natural mating occurred was flushed non-surgically for embryo recovery 7 days post-insemination. Thereafter, all the donor animals were administered with Ilerin. After 48-72 h, they came into heat and mated naturally with yak bulls, and calves were obtained from them after expiration of the normal gestation period. Following superovulation, the average numbers of palpable corpora lutea in the right and left ovaries were 2.25+/-0.6 and 1.75 +/-0.3, respectively. Three embryos were recovered by non-surgical flushing from a single animal. One embryo was transferred to a recipient yak, who produced one female calf after 258 days. This is the first report of production of a yak calf through embryo transfer-technology.
Sarkar
Mihir
M
National Research Centre on Yak (ICAR), Aunachal Pradesh, India. msarkar24@rediffmail.com
Borah
Sagarika
S
Chakraborty
Prithviraj
P
Deka
Bharat
B
Sharma
Babul
B
Duttaborah
Binod
B
Borah
Sanjiv
S
Baruah
Kashyap
K
Ramesha
Kerekoppa
K
Pourochottamne
Ramasamy
R
Kataktakware
Mukund
M
Sarvanan
Buddhi
B
Sengupta
Dilip
D
Das
Sandip
S
Prakash
Bukkaraya Samudram
BS
Bhattacharya
Mohan
M
D000780:Anestrus / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D002417:Cattle / Q000196:embryology / Q000502:physiology; D015237:Dinoprost / Q000031:analogs & derivatives / Q000494:pharmacology; D004624:Embryo Transfer / Q000662:veterinary*; D004971:Estrus / Q000187:drug effects / Q000502:physiology; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D007315:Insemination, Artificial / Q000662:veterinary; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary; D011247:Pregnancy; D018873:Pregnancy Rate; D013480:Superovulation / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
D005300:Fertility Agents, Female; D007987:Gonadotropin-Releasing Hormone; D005640:Follicle Stimulating Hormone; D015237:Dinoprost