Telomerase targeted anticancer bioactive prodrug by antisense-based approach.
A deoxy 11-mer oligonucleotide 5'-GTTAGGGTTAG-3', complementary to a repeat sequence of human telomerase RNA template has been linked through phosphate and a C-2 linker to a bioactive tetraglycine conjugate of curcumin, a well-known antitumor herbal spice component of turmeric. This molecule has been transfected into KB and HeLa cell lines and found to affect cell growth in the former. This DNA-curcumin-tetraglycine acts as a prodrug being targeted by antisense mechanism to telomerase.
Kapoor
Neha
N
Centre for Biotechnology, University of Allahabad, Allahabad, India.
Sharma
Anuj Kumar
AK
Dwivedi
Vishnu
V
Kumar
Anoop
A
Pati
Uttam
U
Misra
Krishna
K
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D016373:DNA, Antisense / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000517:prevention & control*; D011355:Prodrugs / Q000138:chemical synthesis / Q000494:pharmacology*; D019098:Telomerase / Q000037:antagonists & inhibitors / Q000235:genetics*; D014162:Transfection
D000970:Antineoplastic Agents; D016373:DNA, Antisense; D011355:Prodrugs; D019098:Telomerase; D003474:Curcumin