Bioengineered chondrocyte sheets may be potentially useful for the treatment of partial thickness defects of articular cartilage.
Some treatments for full thickness defects of articular cartilage, such as cultured chondrocyte transplantation, have already been done. However, to overcome osteoarthritis, we must further study the partial thickness defect of articular cartilage. It is much more difficult to repair a partial thickness defect because few repairing cells can address such injured sites. We herein show that bioengineered layered chondrocyte sheets using temperature-responsive culture dishes may be a potentially useful treatment for partial thickness defects. We evaluated the property of these sheets using real-time PCR and histological findings, and allografted these sheets to evaluate the effect of treatment using a rabbit partial model. In conclusion, layered chondrocyte sheets were able to maintain the cartilageous phenotype, and could be attached to the sites of cartilage damage which acted as a barrier to prevent a loss of proteoglycan from these sites and to protect them from catabolic factors in the joint.
Kaneshiro
Nagatoshi
N
Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
Sato
Masato
M
Ishihara
Miya
M
Mitani
Genya
G
Sakai
Hideaki
H
Mochida
Joji
J
D000818:Animals; D001698:Biomedical Engineering / Q000379:methods*; D016025:Bone Transplantation; D002357:Cartilage Diseases / Q000628:therapy; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D017690:Cell Transplantation / Q000379:methods; D019902:Chondrocytes / Q000378:metabolism*; D006801:Humans; D009930:Organic Chemicals / Q000737:chemistry; D010641:Phenotype; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature
D009930:Organic Chemicals; C070080:PKH 26