Contrasting pathways of assimilation: stable isotope assessment of fish exposure to pulp mill effluents.
Fish are commonly used for monitoring the quality of waters receiving pulp mill effluents (PMEs). Isotopic assays of fish tissues have the potential to provide empirical evidence to link an effluent source to exposure. We show in a 45-d factorial laboratory experiment that different exposure pathways lead to isotopic signatures in fish tissue. Rainbow trout (Oncorhynchus mykiss) were exposed to 10% PME in three ways; direct exposure through addition of PME to aquaria, indirect exposure through invertebrate food consumption (Chironomus tentans cultured in 10% PME), and a combination of both exposure pathways. Of the four stable isotopes measured (delta13C, delta15N, delta34S, delta37Cl), delta13C, delta34S, and delta37Cl showed significant differences in exposed animal tissues. Delta37Cl of fish muscle tissue showed consistent differences across trophic levels and revealed contrasting pathways of PME exposure. Contrasting delta 37Cl values in C. tentans due to the presence or absence of 10% PME did not translate into delta37Cl differences in fish. Rather, delta37Cl ratios of fish muscle tissue were specifically related to 10% PME exposure in the aquaria (waterborne exposure pathway). Feasible distributions of 37Cl source contributions for observed mixture ratios confirmed that PME accounted for observed differences in delta37Cl among treatments. Direct uptake of chloride ions across gill surfaces is the most likely pathway for assimilation of PME into fish tissues. Considering the variability of PMEs and receiving environments, use of a multi-isotope approach is recommended for tracing exposure of fish. Use of 37Cl should also be considered in light of its alternative assimilation pathway.
Dubé
Monique G
MG
Univ. of Saskatchewan, Toxicology Centre, 44 Campus Dr., Saskatoon, SK S7N 5B3, Canada. monique.dube@usask.ca
Benoy
Glenn A
GA
Wassenaar
Leonard I
LI
D000818:Animals; D001681:Biological Assay / Q000379:methods; D002683:Chironomidae / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D011868:Radioisotopes / Q000032:analysis / Q000378:metabolism; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D014875:Water Pollutants, Radioactive / Q000378:metabolism*
D011868:Radioisotopes; D014874:Water Pollutants, Chemical; D014875:Water Pollutants, Radioactive