Regulation of IL-27p28 gene by lipopolysaccharide in dendritic DC2.4 cells.
To elucidate the regulation of IL-27p28 gene, we analyzed the promoter region of the gene in DC2.4 cells with or without lipopolysaccharide (LPS)-treatment. The results indicate that a region (-648 to -364) of p28 promoter was responsible for LPS-induction. EMSA with DNA probes within the region reveals that binding of GATA motif bound proteins was decreased by LPS-treatment. We identified one of the proteins as non-POU domain-containing octamer binding protein (NonO). Taken together, LPS-induced activation of IL-27p28 gene can be accounted for by the displacement of bound NonO protein from the IL-27p28 promoter.
Kamakura
Maki
M
Laboratory of Molecular Biology, Medical Research Center, Kochi Medical School, Kochi, Japan.
Morisawa
Keiko
K
Komi
Hideyuki
H
Tomatani
Ayako
A
Saito
Fumiji
F
Konishi
Yuko
Y
Jin
Ya
Y
Manabe
Takashi
T
Kuroda
Masayuki
M
Imai
Shosuke
S
Mizuguchi
Hiroyuki
H
Taniguchi
Taketoshi
T
D000818:Animals; D002460:Cell Line; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D011994:Recombinant Proteins / Q000378:metabolism
C506769:Il27 protein, rat; D007378:Interleukins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins