In vitro protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst.
To evaluate the protoscolicidal effects of various concentrations of hypertonic glucose, live protoscolices of sheep were exposed to 10%, 15%, 25% and 50% glucose solutions. Cetrimide (0.5%), silver nitrate (0.5%) and hypertonic saline (20%) were used as positive controls, while physiological saline was used as a negative control. After 1, 2 and 5 min, the protoscolicidal effects were determined by 1% eosin. A 25% glucose solution had no significant protoscolicidal effect. However, a 50% glucose solution revealed higher protoscolicidal effect than 0.5% silver nitrate but weaker effect than 0.5% cetrimide; the effect was comparable with that of 20% hypertonic saline. The results showed that hypertonic glucose solution is highly effective in killing protoscolices of Echinococcus granulosus in vitro.
Hosseini
Seyed Vahid
SV
Department of Surgery, Gastroenterohepatology Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. hoseiniv@sums.ac.ir
Ghanbarzadeh
Kurosh
K
Barzin
Jahra
J
Sadjjadi
Seyed Mahmoud
SM
Tanideh
Nader
N
Mehrabani
Davood
D
D000818:Animals; D004443:Echinococcosis / Q000469:parasitology*; D048209:Echinococcus granulosus / Q000187:drug effects* / Q000302:isolation & purification; D005950:Glucose Solution, Hypertonic / Q000494:pharmacology*; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*
D005950:Glucose Solution, Hypertonic