Agonist of proteinase-activated receptor 2 increases painful behavior produced by alpha, beta-methylene adenosine 5'-triphosphate.
Proteinase-activated receptor (PAR) 2 is expressed in a subset of primary afferent neurons and is involved in inflammatory nociception. The P2X3 ion channel is localized on nociceptors of sensory neurons. Using immunohistochemistry, we showed that many P2X3s are co-expressed with the PAR2 in rat dorsal root ganglia neurons. Nocifensive behavior induced by alphabeta-methylene adenosine 5'-triphosphate (ATP) injection to the hind paw was significantly augmented after the application of PAR2 agonists. Fos expression induced by the alphabeta-methylene ATP injection in dorsal horn neurons was also increased after the pre-application of PAR2 agonists. These findings indicate that PAR2 agonists may potentiate the sensitivity of P2X3 ion channel to noxious stimuli, and the interaction between PAR2 and P2X3 may be an important mechanism underlying inflammatory pain.
Zhu
Wan-Jun
WJ
Department of Anatomy and Neuroscience, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
Dai
Yi
Y
Fukuoka
Tetsuo
T
Yamanaka
Hiroki
H
Kobayashi
Kimiko
K
Obata
Koichi
K
Wang
Shenglan
S
Noguchi
Koichi
K
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000633:toxicity; D000818:Animals; D001522:Behavior, Animal; D004357:Drug Synergism; D015870:Gene Expression / Q000187:drug effects; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D016761:Oncogene Proteins v-fos / Q000378:metabolism; D010146:Pain / Q000139:chemically induced* / Q000503:physiopathology*; D010147:Pain Measurement / Q000379:methods; D020671:Posterior Horn Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044464:Receptor, PAR-2 / Q000819:agonists* / Q000502:physiology; D018048:Receptors, Purinergic P2 / Q000378:metabolism; D058477:Receptors, Purinergic P2X3
D009842:Oligopeptides; D016761:Oncogene Proteins v-fos; C546410:P2rx3 protein, rat; D044464:Receptor, PAR-2; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; C112356:seryl-leucyl-isoleucyl-glycyl--arginyl-leucinamide; D000255:Adenosine Triphosphate; C002630:alpha,beta-methyleneadenosine 5'-triphosphate