Light emitting diodes for auramine O fluorescence microscopic screening of Mycobacterium tuberculosis.
We describe the simple adaptation of a standard fluorescent microscope for illumination using a 'Royal Blue' Luxeon light emitting diode (LED) and demonstrate that this form of illumination is suitable for the detection of auramine O stained Mycobacterium spp. The low cost, low power consumption, safety and reliability of LEDs makes them attractive alternatives to mercury vapour lamps.
Anthony
R M
RM
Department of Biomedical Research, Royal Tropical Institute (KIT), Amsterdam, The Netherlands. r.anthony@kit.nl
Kolk
A H J
AH
Kuijper
S
S
Klatser
P R
PR
D001576:Benzophenoneidum; D004396:Coloring Agents; D004867:Equipment Design; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*
D004396:Coloring Agents; D001576:Benzophenoneidum