title: Influence of environmental conditions on putisolvins I and II production in Pseudomonas putida strain PCL1445.
abstract: Pseudomonas putida strain PCL1445 produces the cyclic lipopeptides putisolvin I and II, which are biosurfactants affecting environmentally important functions including swarming motility, solubility of nutrients and biofilm formation and maintenance. In this study, the effect of relevant nutritional and environmental factors on putisolvin production by PCL1445 was analyzed. Growth and levels of putisolvins I and II biosynthesis were significantly influenced by various carbon sources and by the nature of amino acid supplemented to the culture medium. An increase in the salt concentration of BM medium as well as growth at 1% oxygen significantly increased putisolvin production. In addition, we recently demonstrated that growth at a low temperature has a positive effect on putisolvin production. Analysis of the expression of gacA and gacS in Tn5luxAB mutant strains at a low temperature or under a high salt concentration suggests that biosynthesis of putisolvin is modulated through the GacA/GacS two-component regulatory system in PCL1445 under the tested conditions.
authors.affiliation: Section of Molecular Microbiology, Institute of Biology, Leiden University, Leiden, The Netherlands.
authors.forename: Jean-Frédéric
authors.initials: JF
authors.lastname: Dubern
authors.forename: Guido V
authors.initials: GV
authors.lastname: Bloemberg
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D016958:Pseudomonas putida / Q000378:metabolism* / Q000502:physiology; D013696:Temperature; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010456:Peptides, Cyclic; D015534:Trans-Activators; C481658:putisolvin I; C481659:putisolvin II