Human serum for tissue engineering of human nasal septal cartilage.
OBJECTIVE
To compare the chondrogenic and proliferative effects of pooled human serum (HS) and fetal bovine serum (FBS) on tissue-engineered human nasal septal chondrocytes.


METHODS
Human chondrocytes were expanded for one passage in monolayer in medium supplemented with 10% FBS, 2% HS, 10% HS, or 20% HS. Cells were then suspended in alginate beads for 3D culture for 2 weeks with 10% FBS, 2% HS, 10% HS, or 20% HS.


RESULTS
Monolayer cell yields were greater with HS than FBS. In alginate, cellular proliferation, glycosaminoglycan production per cell, and type II collagen were significantly higher with 10% HS compared to 10% FBS controls.


CONCLUSIONS
HS results in increased proliferation and production of cartilaginous extracellular matrix by tissue-engineered human nasal septal chondrocytes, compared to FBS controls.


CONCLUSIONS
Culture with human serum may facilitate creation of neocartilage constructs that more closely resemble native tissue.
Alexander
Thomas H
TH
Division of Otolaryngology-Head and Neck Surgery, University of California-San Diego, 200 W. Arbor Drive, San Diego, CA 92103, USA. thalexander@ucsd.edu
Sage
August B
AB
Schumacher
Barbara L
BL
Sah
Robert L
RL
Watson
Deborah
D
D000464:Alginates; D000818:Animals; D001769:Blood; D002356:Cartilage / Q000254:growth & development; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology; D020219:Chondrogenesis / Q000502:physiology; D024042:Collagen Type I / Q000032:analysis; D024043:Collagen Type II / Q000032:analysis; D003470:Culture Media; D005109:Extracellular Matrix / Q000737:chemistry; D005312:Fetal Blood; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D009300:Nasal Septum / Q000254:growth & development*; D023822:Tissue Engineering / Q000379:methods*
D000464:Alginates; D024042:Collagen Type I; D024043:Collagen Type II; D003470:Culture Media; D006025:Glycosaminoglycans