title: Structure of the 70S ribosome complexed with mRNA and tRNA.
abstract: The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.
authors.affiliation: MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
authors.forename: Maria
authors.initials: M
authors.lastname: Selmer
authors.forename: Christine M
authors.initials: CM
authors.lastname: Dunham
authors.forename: Frank V
authors.initials: FV
authors.lastname: Murphy
authors.forename: Albert
authors.initials: A
authors.lastname: Weixlbaumer
authors.forename: Sabine
authors.initials: S
authors.lastname: Petry
authors.forename: Ann C
authors.initials: AC
authors.lastname: Kelley
authors.forename: John R
authors.initials: JR
authors.lastname: Weir
authors.forename: V
authors.initials: V
authors.lastname: Ramakrishnan
mesh_terms: D000926:Anticodon; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D003062:Codon; D003460:Crystallization; D018360:Crystallography, X-Ray; D008274:Magnesium / Q000378:metabolism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010458:Peptidyl Transferases / Q000737:chemistry / Q000378:metabolism; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism*; D012358:RNA, Transfer, Met / Q000737:chemistry / Q000378:metabolism; D012360:RNA, Transfer, Phe / Q000737:chemistry / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure*; D016963:Thermus thermophilus / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D000926:Anticodon; D001426:Bacterial Proteins; D003062:Codon; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012358:RNA, Transfer, Met; D012360:RNA, Transfer, Phe; D012269:Ribosomal Proteins; C024988:tRNA, formylmethionine-; D012343:RNA, Transfer; D010458:Peptidyl Transferases; D008274:Magnesium