title: An integrated proteomic workflow for two-dimensional differential gel electrophoresis and robotic spot picking.
abstract: New technologies have advanced the field of proteomics, and a number of companies have developed innovative platforms to drive this research. However, significant challenges are often encountered when trying to integrate complementary technologies from multiple manufacturers. We have developed a software and hardware solution to integrate the Ettan two-dimensional difference gel electrophoresis (2-D DIGE) system (GE Healthcare) with the Investigator ProPic spot picking robot (Genomic Solutions). We have analyzed protein sample preparations from bacterial and mammalian sources to demonstrate a new workflow with increased throughput for gel-based proteomics.
authors.affiliation: New Technologies Engineering Division, Biosciences Directorate, Lawrence Livermore National Laboratory, 7000 East Avenue, L-452, Livermore, California 94551, USA.
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Mahnke
authors.forename: Todd H
authors.initials: TH
authors.lastname: Corzett
authors.forename: Sandra L
authors.initials: SL
authors.lastname: McCutchen-Maloney
authors.forename: Brett A
authors.initials: BA
authors.lastname: Chromy
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods* / Q000639:trends; D012371:Robotics / Q000379:methods*; D012984:Software
chemical_list: D011506:Proteins