title: Surveyor nuclease-based genotyping of SNPs.
abstract: Surveyor nuclease, a single-strand-specific endonuclease that cleaves DNA at the 3' side of a base-mismatch site in both DNA strands, can be used for genotyping of SNPs by preparing two different types of a heteroduplex: one from mutant DNA fragments and the other from a mixture of wild-type and mutant fragments. We show an example of this technique and propose that it can be used with some advantages instead of RFLP analysis.
authors.affiliation: Central Clinical Laboratory, Nara Medical University Hospital, Shijo-Cho 840, Kashihara, Nara 634-8522, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Mitani
authors.forename: Shinobu
authors.initials: S
authors.lastname: Tanaka
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Okamoto
mesh_terms: D004247:DNA / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000378:metabolism*; D005821:Genetic Techniques; D005838:Genotype; D009154:Mutation / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D004247:DNA; D004720:Endonucleases