Proteomic analysis of effect of hyperthermia on spermatogenesis in adult male mice.
We characterized cellular and molecular mechanisms involved in spermatogenesis following short-term heat exposure of murine testis. For these studies, we utilized a proteomic approach with two-dimensional gel electrophoresis (2DE) analyses and mass spectroscopic identification of proteins with altered expression in mouse testes at different times after heat shock. We established a proteome reference map from 7-wk-old mouse testis linked to a federated proteome database. We used these tools to analyze quantitative variations in the tissue over a time course of 0.5, 2, 6, and 12 h following heat exposure. We separated 108 protein spots expressed differentially between the heat shock tissues and the control mouse testes. Of these spots, we identified 36 by comparing with the control reference map. We then focused on the heterogeneous nuclear ribonucleoproteins (hnRNPs) and the chaperonins containing t-complex polypeptide-1 (CCT). Further analysis in this heat-shocked model suggests numerous potential mechanisms for heat shock-induced spermatogenic disorder.
Zhu
Ye-Fei
YF
Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, PR China.
Cui
Yu-Gui
YG
Guo
Xue-Jiang
XJ
Wang
Lei
L
Bi
Ye
Y
Hu
Yan-Qiu
YQ
Zhao
Xin
X
Liu
Quan
Q
Huo
Ran
R
Lin
Min
M
Zhou
Zuo-Min
ZM
Sha
Jia-Hao
JH
D000818:Animals; D015153:Blotting, Western; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D006979:Hyperthermia, Induced / Q000009:adverse effects*; D007150:Immunohistochemistry; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000502:physiology*
D011506:Proteins