The influence of polychlorinated biphenyls (PCBs) and phytoestrogens in vitro on functioning of reproductive tract in cow.
Ovarian, endometrial and myometrial cells and strips of longitudinal myometrium from cows on defined days of estrous cycle were treated for 24-72 h with different doses (1-100 ng/ml) of PCBs mixture (Aroclor 1248) or with one of PCB congeners (126, 77, 153). The administered doses of PCBs neither affected the viability of cells nor influenced the ovarian steroidogenesis as measured by progesterone (P(4)), estradiol (E(2)) and testosterone secretion from luteal, granulosa and theca cells, respectively. In contrast, PCBs clearly inhibited a FSH and LH-stimulated effect on steroids secretion from granulosa and luteal cells. Moreover, PCBs significantly stimulated oxytocin (OT) secretion from the studied ovarian cells, and at least part of this effect is elicited through activation of glucocorticoid receptors. Further, PCBs were found to increase basal intracellular concentrations of Ca(2+) and both spontaneous and OT-stimulated contractions of myometrial strips. Concomitantly, PCBs increased endometrial secretion of PGF(2alpha), hence the ratio of PGF(2alpha):PGE(2) was also increased. Phytoestrogens (genistein, daidzein, coumestrol), with a different intensity, reduced the effect of PCBs on PGF(2alpha) secretion and myometrial contractions. Genistein inhibited PCBs' effect on OT secretion from granulosa cells, while PCB's effect on OT release from luteal cells was reduced mainly by genistein and daidzein. We conclude that PCBs can impair both ovarian functioning and uterine contractility, while phytoestrogens are able to reduce this effect.
Kotwica
Jan
J
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland. janko@pan.olsztyn.pl
Wróbel
Michał
M
Młynarczuk
Jarosław
J
D000818:Animals; D002417:Cattle / Q000502:physiology*; D004347:Drug Interactions / Q000502:physiology*; D005260:Female; D010053:Ovary / Q000187:drug effects; D048789:Phytoestrogens / Q000494:pharmacology*; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D012098:Reproduction / Q000187:drug effects*; D014599:Uterus / Q000187:drug effects
D048789:Phytoestrogens; D011078:Polychlorinated Biphenyls