[Influence of bifidobacterial whole peptidoglycan on cellular immune response induced by recombinant HBsAg].
OBJECTIVE
To explore the effect of bifidobacterial whole peptidoglycan (BWPG) on cellular immune response to recombinant hepatitis B surface antigen (rHBsAg).


METHODS
BWPG was used as an adjuvant of rHBsAg to immunize BALB/c mice. The growth state and development of central lymphoid organ of immunized mice were observed. Then the killing activities of NK cells and CTLs, the proliferation and cytokine production of splenic lymphocytes were examined.


RESULTS
After immunization with BWPG, no visible side effect was observed in immunized mice. The thymus and spleen tissues assumed hyperplasia appearance. The killing activities of NK cells and CTLs in spleen were enhanced. The proliferation of splenic lymphocytes was active and cytokine production was increased.


CONCLUSIONS
BWPG can promote hyperplasia of central lymphoid organ and enhance the cellular immune response to rHBsAg.
Wang
Yue
Y
Department of Clinical Microbiololgy, Chongqing University of Medical Sciences, Chongqing 400016, China. wangyue_26@hotmail.com
Xie
Cheng-bin
CB
Liu
Ming-fang
MF
Chen
Shu-hui
SH
D000818:Animals; D001644:Bifidobacterium / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D017325:Hepatitis B Vaccines; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010457:Peptidoglycan / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000276:immunology
D016207:Cytokines; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D010457:Peptidoglycan; D011994:Recombinant Proteins