title: An extract of Polygonum multiflorum protects against free radical damage induced by ultraviolet B irradiation of the skin.
abstract: Over the last decades, the incidence of ultraviolet B (UVB)-related skin problems has been increasing. Damages induced by UVB radiation are related to mutations that occur as a result of direct DNA damage and/or the production of reactive oxygen species. We investigated the anti-oxidant effects of a Polygonum multiflorum thumb extract against skin damage induced by UVB irradiation. Female SKH-1 hairless mice were divided into three groups: control (N = 7), distilled water- (N = 10), and P. multiflorum extract-treated (PM, N = 10) groups. The PM (10 g) was extracted with 100 mL distilled water, cryo-dried and 9.8 g was obtained. The animals received a topical application of 500 microL distilled water or PM extract (1, 2, 4, 8, and 16%, w/v, dissolved in distilled water) for 30 min after UVB irradiation (wavelength 280-320 nm, 300 mJ/cm(2); 3 min) of the dorsal kin for 14 days, and skin immunohistochemistry and Cu,Zn-superoxide dismutase (SOD1) activity were determined. SOD1 immunoreactivity, its protein levels and activities in the skin were significantly reduced by 70% in the distilled water-treated group after UVB irradiation compared to control. However, in the PM extract-treated groups, SOD1 immunoreactivity and its protein and activity levels increased in a dose-dependent manner (1-16%, w/v, PM extract) compared to the distilled water-treated group. SOD1 protein levels and activities in the groups treated with 8 and 16%, w/v, PM extract recovered to 80-90% of the control group levels after UVB. These results suggest that PM extract strongly inhibits the destruction of SOD1 by UV radiation and probably contains anti-skin photoaging agents.
authors.affiliation: Central Research Center, Natural F & P Co., Ltd., Chunchon, South Korea.
authors.forename: I K
authors.initials: IK
authors.lastname: Hwang
authors.forename: K-Y
authors.initials: KY
authors.lastname: Yoo
authors.forename: D W
authors.initials: DW
authors.lastname: Kim
authors.forename: S J
authors.initials: SJ
authors.lastname: Jeong
authors.forename: C-K
authors.initials: CK
authors.lastname: Won
authors.forename: W-K
authors.initials: WK
authors.lastname: Moon
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: D Y
authors.initials: DY
authors.lastname: Kwon
authors.forename: M H
authors.initials: MH
authors.lastname: Won
authors.forename: D-W
authors.initials: DW
authors.lastname: Kim
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D015153:Blotting, Western; D005260:Female; D051379:Mice; D008812:Mice, Hairless; D010936:Plant Extracts / Q000627:therapeutic use; D028502:Polygonum / Q000737:chemistry*; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000517:prevention & control*; D012867:Skin / Q000528:radiation effects*; D013482:Superoxide Dismutase / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1