Study of immobilized and extracellular saccharase of watermelon.
A simple, rapid and reproducible procedure for the identification and determination of extracellular saccharase from culture medium of watermelon cell suspension cultures is described. The culture medium (without cells) was used for the identification and determination of extracellular enzyme activity. Intracellular activity was estimated from the cell suspension. Watermelon cell suspension was permeabilized by Tween 80 and immobilized by glutaraldehyde. The highest saccharase activity was at pH 4.6 at a temperature of 50 degrees C. The hydrolysis of substrate was linear 5h after reaching 60% conversion. The cells had high saccharase activity and good stability, and in long-term storage they showed convenient physico-mechanical properties.
Stano
Ján
J
Food Research Institute, P.O. Box 25, Priemyselná 4, 824 75 Bratislava 26, Slovak Republic.
Siekel
Peter
P
Micieta
Karol
K
Barth
Alfred
A
D002478:Cells, Cultured; D029773:Citrullus / Q000166:cytology* / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D010539:Permeability; D043324:beta-Fructofuranosidase / Q000378:metabolism*
D004800:Enzymes, Immobilized; D005632:Fructose; D043324:beta-Fructofuranosidase; D005947:Glucose; D005690:Galactose