title: Prenatal initiation of endotoxin airway exposure prevents subsequent allergen-induced sensitization and airway inflammation in mice.
abstract: BACKGROUND
  New preventive strategies against the development of allergic diseases focus on potentially immunomodulatory components, such as bacterial LPSs. Optimal time frames for initiating immunomodulation to receive a sufficient effect against allergen sensitization are still unclear.
  
  
  OBJECTIVE
  Using a mouse model, we investigated the influence of prenatal LPS exposure on later allergen-mediated sensitization and airway inflammation in the offspring.
  
  
  METHODS
  Pregnant BALB/c mice were repeatedly exposed to aerosolized LPS (LPS Escherichia coli; 3x per week, day 7 of gestation time up to delivery). Some of the offspring were further exposed to aerosolized LPS before allergen sensitization with ovalbumin (OVA; administered intraperitoneally day 28 up to day 42) and OVA airway challenges (days 56-58). Positive control animals were placebo exposed to PBS instead of LPS, and negative control animals were first placebo exposed and later placebo sensitized with PBS instead of OVA.
  
  
  RESULTS
  Compared with positive control animals, prenatal LPS exposure suppressed (1) allergen-specific sensitization (IgE production), (2) eosinophilic airway inflammation (reduced numbers of eosinophils in bronchoalveolar lavage fluids), and (3) in vivo airway reactivity in response to methacholine. These effects occurred only when prenatal was combined with further postnatal LPS exposure. Suppression of allergen-mediated inflammatory responses was associated with increased Toll-like receptor and T-bet expression by lung tissues and a shift toward predominantly T(H)1 immune responses in spleen cells cultured with OVA in vitro.
  
  
  CONCLUSIONS
  Prenatal initiated and postnatal sustained LPS exposure increased endotoxin susceptibility and prevented later allergen sensitization in offspring through inhibition of T(H)2 immune responses.
  
  
  CONCLUSIONS
  Immunomodulation with bacterial compounds during gestation time might be an effective mode for first-step primary prevention against allergic diseases.
authors.affiliation: Department of Pediatric Pneumology and Immunology, University Hospital Charité, Berlin, Germany. kerstin.gerhold@charite.de
authors.forename: Kerstin
authors.initials: K
authors.lastname: Gerhold
authors.forename: Angela
authors.initials: A
authors.lastname: Avagyan
authors.forename: Christine
authors.initials: C
authors.lastname: Seib
authors.forename: Remo
authors.initials: R
authors.lastname: Frei
authors.forename: Johanna
authors.initials: J
authors.lastname: Steinle
authors.forename: Birgit
authors.initials: B
authors.lastname: Ahrens
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Dittrich
authors.forename: Katharina
authors.initials: K
authors.lastname: Blumchen
authors.forename: Roger
authors.initials: R
authors.lastname: Lauener
authors.forename: Eckard
authors.initials: E
authors.lastname: Hamelmann
mesh_terms: D000336:Aerosols; D000485:Allergens / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D002454:Cell Differentiation / Q000276:immunology; D049109:Cell Proliferation; D004731:Endotoxins / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D007249:Inflammation / Q000276:immunology / Q000517:prevention & control; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control; D012130:Respiratory Hypersensitivity / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D018417:Th1 Cells / Q000276:immunology / Q000473:pathology; D018418:Th2 Cells / Q000276:immunology / Q000473:pathology
chemical_list: D000336:Aerosols; D000485:Allergens; D004731:Endotoxins; D008070:Lipopolysaccharides