A standard protocol for obtaining fish chromosomes under post-mortem conditions.
In order to improve cytogenetical analyses on fish, especially focusing on delicate and rare species, we have adopted a new in vitro methodology using dead animals. The results obtained from 24 neotropical freshwater and marine fish species demonstrate that chromosomes can be obtained under post-mortem conditions. Significantly, the samples analyzed provided reliable cytogenetical data in nearly all cases. Other advantages of this new methodology are also discussed.
de Cáscia Barreto Netto
Maria Rita
MR
Laboratório de Biologia e Genética de Peixes, Instituto de Biociências, Universidade Estadual Paulista, 18 618-000 Botucatu, SP, Brazil.
Pauls
Erica
E
de Mello Affonso
Paulo Roberto A
PR
D000818:Animals; D002875:Chromosomes; D020732:Cytogenetic Analysis / Q000379:methods* / Q000592:standards*; D005399:Fishes / Q000235:genetics*; D008853:Microscopy