[Effects of gavage with lactococcus lactis recombinant heme oxygenase-1 gene on inflammation of intestine and bacterial translocation in rats with hemorrhagic shock].
OBJECTIVE
To evaluate the effects of gavage with lactococcus lactis (L. Lactis) recombinant heme oxygenase-1 (HO-1) gene on alleviation of intestinal inflammation and protection of the intestinal mucosa in rats with hemorrhagic shock.


METHODS
A model of rats with hemorrhagic shock was reproduced in 30 healthy SD male rats. The rats were randomly divided into the L. Lactis recombinant HO-1 gene group (HO group, n=10), L. Lactis group (LL group, n=10) and phosphate buffer group (PBS group, n=10). These agents were respectively gavaged 24 hours before the experiment. Rats were re-anesthetized 1 hour after fluid resuscitation. The mortality, myeloperoxidase (MPO) activity, bacterial translocation, the pathologic changes, the contents of HO-1, tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) in the intestine were determined and compared.


RESULTS
Compared with LL group and PBS group, the mortality, Chiu's grade and the bacterial translocation rate of HO group were significantly decreased (all P<0.05) but the content of HO-1 and the level of IL-10 in HO group were markedly increased (both P<0.05). Compared with HO group and LL group, the MPO activity of PBS group was obviously increased (P<0.05).


CONCLUSIONS
The recombinant L. Lactis has the effect to deliver HO-1, which has protective effect on the intestinal mucosa in lessening the inflammation of the intestine and the incidence of bacterial translocation.
Gao
Xin-yue
XY
Department of Anesthesiology, The Fourth People's Hospital, Wuxi 214062, Jiangsu, China. gaodays@163.com
Wu
Chang-yi
CY
Zhou
Qiang
Q
Pang
Qing-feng
QF
Zeng
Yin-ming
YM
D000818:Animals; D018988:Bacterial Translocation; D003131:Combined Modality Therapy; D019610:Cytoprotection; D004195:Disease Models, Animal; D004751:Enteritis / Q000209:etiology / Q000517:prevention & control; D015316:Genetic Therapy; D005822:Genetic Vectors; D051547:Heme Oxygenase-1 / Q000235:genetics*; D007413:Intestinal Mucosa / Q000382:microbiology*; D013294:Lactococcus lactis / Q000235:genetics*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000150:complications / Q000382:microbiology / Q000628:therapy*
D051547:Heme Oxygenase-1