Metschnikowia sinensis sp. nov., Metschnikowia zizyphicola sp. nov. and Metschnikowia shanxiensis sp. nov., novel yeast species from jujube fruit.
Eight yeast strains were isolated from jujube fruit surfaces collected in Shanxi and Shandong Provinces, China. All eight strains produced needle-shaped ascospores under suitable conditions. Three separate groups, representing three novel species in the genus Metschnikowia, were recognized by sequence comparisons of the 26S rDNA D1/D2 domain and internal transcribed spacer (ITS) region. The names Metschnikowia sinensis sp. nov. (type strain XY103(T)=AS 2.3110(T)=CBS 10357(T)), Metschnikowia zizyphicola sp. nov. (type strain XY201(T)=AS 2.3111(T)=CBS 10358(T)) and Metschnikowia shanxiensis sp. nov. (type strain XY801(T)=AS 2.3112(T)=CBS 10359(T)) are proposed for the three novel species. Phylogenetic analysis of the 26S rDNA D1/D2 domain sequence showed that these three novel species are clustered in a clade together with the previously described species Metschnikowia fructicola, Metschnikowia andauensis, Metschnikowia pulcherrima and Metschnikowia chrysoperlae.
Xue
Meng-Lin
ML
Department of Plant Pathology, China Agricultural University, Beijing 100094, China.|College of Life Science, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China.
Zhang
Li-Qun
LQ
Department of Plant Pathology, China Agricultural University, Beijing 100094, China.
Wang
Qi-Ming
QM
Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China.
Zhang
Ji-Shu
JS
College of Life Science, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China.
Bai
Feng-Yan
FY
Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China.
D002681:China; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000032:analysis* / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification / Q000302:isolation & purification*; D031957:Ziziphus / Q000382:microbiology*
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S