Phosphorylase coupling as a tool to convert cellobiose into amylose.
This work aims to establish the enzymatic process to produce amylose from cellobiose. Incubation of cellobiose with cellobiose phosphorylase and alpha-glucan phosphorylase in the presence of maltotetraose and a catalytic amount of inorganic phosphate at 45 degrees C for 16 h resulted in the production of linear alpha-1,4-glucan with a 19.3% (w/v, against cellobiose weight) yield. The yield was successfully improved (32.4%) when mutarotase and glucose oxidase were added to remove glucose in the reaction mixture. The weight-average molecular weight of the product was precisely controlled from 42 to 720 kDa by changing the initial molar ratio of cellobiose to maltotetraose. The combined use of two different phosphorylases should be a useful tool in converting beta-1,4-linked-polysaccharide into alpha-1,4-linked-polysaccharide.
Ohdan
Kohji
K
Biochemical Research Laboratory of Ezaki Glico Co. Ltd., 4-6-5 Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan. odan-kouji@glico.co.jp
Fujii
Kazutoshi
K
Yanase
Michiyo
M
Takaha
Takeshi
T
Kuriki
Takashi
T
D000688:Amylose / Q000737:chemistry*; D002238:Carbohydrate Epimerases / Q000737:chemistry; D002475:Cellobiose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D005964:Glucosyltransferases / Q000737:chemistry*; D006005:Phosphorylases / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
D011994:Recombinant Proteins; D002475:Cellobiose; D000688:Amylose; D005949:Glucose Oxidase; D005964:Glucosyltransferases; D006005:Phosphorylases; C034714:cellobiose phosphorylase; D002238:Carbohydrate Epimerases; C019646:aldose 1-epimerase