title: Construction of a sIgA-enhancing anti-Porphyromonas gingivalis FimA vaccine and nasal immunization in mice.
abstract: Porphyromonas gingivalis is implicated in the etiology of chronic periodontitis. Fimbriae are one of several critical surface virulence factors of P. gingivalis. Interleukin 15 (IL-15) is a critical important cytokine for the differentiation of B-1 cells into IgA-inducing cells in mucosal tissues and the proliferation of B cells. The present study constructed a co-expression plasmid pIRES-fimA:IL-15 encoding fimbrinllin (FimA), a subunit of fimbriae and IL-15 as a sIgA-enhancing anti-P. gingivalis FimA vaccine. The plasmid pIRES-fimA:IL-15 was transfected to CHO cells. The expressions of FimA and IL-15 in CHO cells were verified by Western blot and ELISA. Mice were immunized with pIRES-fimA:IL-15 via nasal or intramucusal route. The results showed that nasal immunization was capable of promoting Ag-specific immune responses in the oral region as well as systemic immunity. When immunized via nasal route, IL-15 expressed by the plasmid enhanced FimA-specific sIgA antibody response. In conclusion, a co-expression plasmid pIRES-fimA:IL-15 has been constructed, and when immunized via nasal route, antigen-specific sIgA antibody response could be modulated positively in immunized mice.
authors.affiliation: Stomatology Hospital of Shandong University, Jinan 250012, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Guo
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
authors.forename: Guangshui
authors.initials: G
authors.lastname: Jiang
authors.forename: Pishan
authors.initials: P
authors.lastname: Yang
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D016866:Bacteroidaceae Infections / Q000276:immunology / Q000517:prevention & control; D036781:Fimbriae Proteins / Q000235:genetics / Q000276:immunology*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D018928:Immunity, Mucosal / Q000276:immunology; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D019409:Interleukin-15 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D016966:Porphyromonas gingivalis / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D007071:Immunoglobulin A, Secretory; D019409:Interleukin-15; D019444:Vaccines, DNA; C063055:fimbrillin; D036781:Fimbriae Proteins