Pilot study of a commercialized human papillomavirus (HPV) genotyping assay: comparison of HPV risk group to cytology and histology.
We evaluated a commercialized PCR assay, Linear Array, that detects 37 human papillomavirus (HPV) genotypes, using a sample of liquid cytology specimens (n = 534). We found a strong association of an increasing level of HPV risk (HPV type 16 [HPV16] > HPV18 > other carcinogenic types > noncarcinogenic types > negative specimens) with increasing severities of cytologic interpretations (P(Trend) < 0.0005) and histologic diagnoses (P(Trend) < 0.0005).
Castle
Philip E
PE
Division of Cancer Epidemiology, National Cancer Institute, NIH, DHHS, Bethesda, MD 20892-7234, USA. castlep@mail.nih.gov
Sadorra
Mark
M
Garcia
Francisco
F
Holladay
E Blair
EB
Kornegay
Janet
J
D003581:Cytodiagnosis; D005260:Female; D005838:Genotype; D006801:Humans; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics; D030361:Papillomavirus Infections / Q000473:pathology* / Q000821:virology; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000821:virology