Preclinical investigations of a medium-chain triglyceride:fish oil emulsion. I. Effects of bovine milk lipoprotein lipase on lipid composition.
The bolus intravenous injection of a novel medium-chain triglyceride:fish oil emulsion (MCT:FO, 8:2, w:w) was recently found to increase within 60 min the leucocyte and platelet phospholipid content of long-chain polyunsaturated omega3 fatty acids. The present report deals with the effects of bovine milk lipoprotein lipase on the lipid composition of this emulsion. The results are compared to those obtained with either a pure fish oil emulsion or a medium-chain triglyceride: long-chain triglyceride:fish oil emulsion (MLF, 5:4:1). Emphasis is placed on i) differences in the fate of distinct fatty acids initially present in the triglycerides, di glycerides and phospholipids, ii) the generation of unesterified fatty acids relative to their initial content in each emulsion, and iii) the time course for these various events. The comparison between the three emulsions under consideration also provides information relevant to their respective sensitivity to lipoprotein lipase and suitability in terms of the generation of distinct unesterified fatty acids, including long-chain polyunsaturated omega3 fatty acids. Furthermore, attention is drawn to the greater efficiency for the hydrolysis of fatty acids from diglycerides as compared to triglycerides and a transient increase in the paired C8:0/C10:0 ratio in the diglycerides generated from the MCT:FO or MLF emulsion. The present study thus affords novel information relevant to the possible use of the MCT:FO emulsion in human subjects.
Carpentier
Yvon A
YA
Laboratory of Experimental Surgery L. Deloyers, Brussels Free University, B-1070 Brussels, Belgium.
Dupont
Isabelle
I
Portois
Laurence
L
Malaisse
Willy J
WJ
D000818:Animals; D004075:Diglycerides / Q000737:chemistry; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005217:Fat Emulsions, Intravenous / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D005395:Fish Oils / Q000737:chemistry*; D006868:Hydrolysis; D008055:Lipids / Q000737:chemistry*; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008892:Milk / Q000201:enzymology; D050178:Monoglycerides / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D013997:Time Factors; D014280:Triglycerides / Q000737:chemistry*
D004075:Diglycerides; D005217:Fat Emulsions, Intravenous; D005227:Fatty Acids; D005395:Fish Oils; D008055:Lipids; D050178:Monoglycerides; D010743:Phospholipids; D014280:Triglycerides; D008071:Lipoprotein Lipase