Proteomic analysis of root meristems and the effects of acetohydroxyacid synthase-inhibiting herbicides in the root of Medicago truncatula.
Quantitative proteome analyses of meristematic and nonmeristematic tissues from Medicago truncatula primary and lateral roots and meristem tissues from plants treated with acetohydroxyacid synthase-inhibiting herbicides were made. The accumulation of 81 protein spots changed in meristematic and nonmeristematic tissues and 51 protein spots showed significant changes in accumulation in herbicide-treated meristems. Identified proteins indicate two trends, (i) increased accumulation of cell division and redox-mediating proteins in meristems compared to nonmeristematic tissues and (ii) increased accumulation of pathogenesis-related and decreased accumulation of metabolic proteins in herbicide-treated roots.
Holmes
Peta
P
ARC Centre of Excellence for Integrative Legume Research, Genomic Interactions Group, Research School of Biological Sciences, Australian National University, Canberra ACT 2601, Australia.
Farquharson
Ryan
R
Hall
Prudence J
PJ
Rolfe
Barry G
BG
D000095:Acetolactate Synthase / Q000037:antagonists & inhibitors; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D006540:Herbicides / Q000633:toxicity*; D013058:Mass Spectrometry; D046913:Medicago truncatula / Q000235:genetics*; D018519:Meristem / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods
D006540:Herbicides; D010940:Plant Proteins; D000095:Acetolactate Synthase