Xenopus Tbx6 mediates posterior patterning via activation of Wnt and FGF signalling.
In vertebrates, the patterning of anterior-posterior (AP) axis is a fundamental process during embryogenesis. Wnt and FGF signalling pathways play important roles in regulating the patterning of embryo AP axis. Mouse Tbx6 encodes a transcription factor that has been demonstrated to be involved in the specification of the posterior tissue in mouse embryonic body. Here, we prove that morpholino-induced knockdown of XTbx6 impairs posterior development, indicating the requirement of XTbx6 in this process. Meanwhile, gain of XTbx6 function is sufficient to induce ectopic posterior structures in Xenopus embryos. Furthermore, XTbx6 activates the expression of Xwnt8 and FGF8, which are two mediators of posterior development, suggesting a mechanism by which XTbx6 modulates posterior patterning via Wnt and FGF signalling pathway activation.
Lou
Xin
X
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China.
Fang
Panfeng
P
Li
Shuangwei
S
Hu
Rui-Ying
RY
Kuerner
Klaus-Michael
KM
Steinbeisser
Herbert
H
Ding
Xiaoyan
X
D000818:Animals; D019521:Body Patterning; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D051379:Mice; D009024:Morphogenesis; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism*; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism
D016376:Oligonucleotides, Antisense; D020825:T-Box Domain Proteins; C491181:Tbx6 protein, Xenopus; D051153:Wnt Proteins; D029867:Xenopus Proteins; D005346:Fibroblast Growth Factors