title: Ultra-high resolution array painting facilitates breakpoint sequencing.
abstract: OBJECTIVE
  To describe a considerably advanced method of array painting, which allows the rapid, ultra-high resolution mapping of translocation breakpoints such that rearrangement junction fragments can be amplified directly and sequenced.
  
  
  METHODS
  Ultra-high resolution array painting involves the hybridisation of probes generated by the amplification of small numbers of flow-sorted derivative chromosomes to oligonucleotide arrays designed to tile breakpoint regions at extremely high resolution.
  
  
  CONCLUSIONS
  How ultra-high resolution array painting of four balanced translocation cases rapidly and efficiently maps breakpoints to a point where junction fragments can be amplified easily and sequenced is demonstrated. With this new development, breakpoints can be mapped using just two array experiments: the first using whole-genome array painting to tiling resolution large insert clone arrays, the second using ultra-high-resolution oligonucleotide arrays targeted to the breakpoint regions. In this way, breakpoints can be mapped and then sequenced in a few weeks.
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.
authors.forename: S M
authors.initials: SM
authors.lastname: Gribble
authors.forename: D
authors.initials: D
authors.lastname: Kalaitzopoulos
authors.forename: D C
authors.initials: DC
authors.lastname: Burford
authors.forename: E
authors.initials: E
authors.lastname: Prigmore
authors.forename: R R
authors.initials: RR
authors.lastname: Selzer
authors.forename: B L
authors.initials: BL
authors.lastname: Ng
authors.forename: N S W
authors.initials: NS
authors.lastname: Matthews
authors.forename: K M
authors.initials: KM
authors.lastname: Porter
authors.forename: R
authors.initials: R
authors.lastname: Curley
authors.forename: S J
authors.initials: SJ
authors.lastname: Lindsay
authors.forename: J
authors.initials: J
authors.lastname: Baptista
authors.forename: T A
authors.initials: TA
authors.lastname: Richmond
authors.forename: N P
authors.initials: NP
authors.lastname: Carter
mesh_terms: D000328:Adult; D002675:Child, Preschool; D019457:Chromosome Breakage; D002874:Chromosome Mapping / Q000379:methods*; D020223:Chromosome Painting / Q000379:methods*; D002877:Chromosomes, Human / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D014178:Translocation, Genetic