title: Identifying microRNA regulators of cell death in Drosophila.
abstract: Animal genomes contain on the order of at least hundreds of microRNAs (miRNAs). Although most remain uncharacterized, it is already clear that miRNAs regulate many biological processes. A number of Drosophila miRNAs have been identified as likely cell death regulators, but functions for most have simply not been explored. Here we describe a protocol for identifying miRNAs that can act as cell death regulators. We also describe a simple protocol for testing roles for mRNAs identified as candidate miRNA targets using computational or other approaches.
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena, CA, USA.
authors.forename: Chun-Hong
authors.initials: CH
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Guo
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Hay
mesh_terms: D000818:Animals; D016923:Cell Death / Q000502:physiology; D019295:Computational Biology / Q000379:methods; D004330:Drosophila / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*
chemical_list: D035683:MicroRNAs