The use of chitosan modified with glutaraldehyde and glyoxal as chromatographic support for the separation of flavonoids from Aleurites moluccana leaves.
This paper describes the preparation of chitosan modified with dialdehydes, glutaraldehyde (CH-Glu) and glyoxal (CH-Gly) and its application in the isolation of the flavonoids swertisin and 2"-O-rhamnosylswertisin from A. mollucana. The additional non-polar alkyl groups increase the hydrophobicity of the sorbent. The results show that the separation was mediated by hydrophobic interaction (CH-Glu), as well as hydrogen bonding, between phenolic OH or rhamnosil residues from the flavonoids, and the free amine groups (CH-Gly).
Morsch
M
M
Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Curso de Farmácia/CCS, Universidade do Vale do Itajaí, Brasil.
Girardi
L G J
LG
Cechinel-Filho
V
V
Meyre-Silva
C
C
Rodrigues
C A
CA
D031284:Aleurites / Q000737:chemistry*; D002240:Carbohydrate Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D048271:Chitosan / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D005976:Glutaral / Q000737:chemistry*; D006037:Glyoxal / Q000737:chemistry*; D006860:Hydrogen Bonding; D008969:Molecular Sequence Data
D005419:Flavonoids; D006037:Glyoxal; D048271:Chitosan; D005976:Glutaral