Centrifugal partition chromatography as a tool for preparative purification of pea albumin with enhanced yields.
A new procedure including the use of centrifugal partition chromatography (CPC) is proposed to purify PA1b and its isoforms. These pea (Pisum sativum L.) seed proteins are toxic against weevils and can be used as an environment-friendly insecticide. CPC was applied to a whole albumin fraction prepared from pea flour. The butanol:aqueous TFA system used in CPC allowed the separation of PA1b from other albumins and a degree of purification above 95%. Compared to analytical procedures based on methanol extraction, anion exchange and then reversed-phase chromatography (RPC), CPC recovered PA1b in much better yield, which is indispensable for large-scale purification of a biodegradable insecticide.
Bérot
Serge
S
Institut National de la Recherche Agronomique - Unité de recherche Biopolymères, Interactions, Assemblages, 44316 Nantes Cedex 3, France. berot@nantes.inra.fr
Le Goff
Elisabeth
E
Foucault
Alain
A
Quillien
Laurence
L
D000418:Albumins / Q000302:isolation & purification*; D002498:Centrifugation; D002853:Chromatography, Liquid / Q000379:methods*; D007306:Insecticides / Q000302:isolation & purification; D018532:Pisum sativum / Q000737:chemistry*
D000418:Albumins; D007306:Insecticides