Spatial and temporal control of laminin-332 (5) and -511 (10) expression during induction of anagen hair growth.
Basement membrane plays important roles in hair growth. We characterized changes in laminin isoform expression during hair cycling. At the mRNA level, laminin-511 (10) expression underwent a steady increase during anagen stages. In contrast, laminin-332 (5) expression was initially upregulated in outer root sheath (ORS) keratinocytes at anagen II and then transiently downregulated. Laminin-332 significantly increased coincident with the signal in inner root sheath and hair matrix cells after anagen IV. Levels of laminin-332 proteins were also upregulated at late anagen I-III but dropped after anagen IV. This decrease coincided with increased levels of mRNA encoding the two proteases, membrane type 1 metalloproteinase and bone morphogenetic protein 1, involved in laminin-332 processing. Immunohistochemistry demonstrated that laminin-332 and alpha6 beta4 integrin were well colocalized, but their signals were remarkably decreased in the lower half of follicles after anagen VI. Consistent with these data, ultrastructurally mature hemidesmosomes were seen in ORS keratinocytes at anagen II, whereas at anagen VI, only fragmental hemidesmosomes were present. In hair follicle culture, laminin-511 (10)/521 (11)-rich human placental laminin enhanced hair growth, whereas recombinant laminin-332 antagonized hair growth induced by laminin-511. Our results indicate a positive role for laminin-511 and a negative role for laminin-332 on hair growth.
Sugawara
Koji
K
Department of Dermatology, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Osaka, Japan.
Tsuruta
Daisuke
D
Kobayashi
Hiromi
H
Ikeda
Kazuo
K
Hopkinson
Susan B
SB
Jones
Jonathan C R
JC
Ishii
Masamitsu
M
D000818:Animals; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000494:pharmacology; D049109:Cell Proliferation; D002478:Cells, Cultured; D006197:Hair / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D018859:Hair Follicle / Q000378:metabolism / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D039201:Integrin alpha3beta1 / Q000096:biosynthesis; D039161:Integrin alpha6beta4 / Q000096:biosynthesis; D007797:Laminin / Q000096:biosynthesis* / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D020033:Protein Isoforms / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097794:Kalinin
D015815:Cell Adhesion Molecules; D039201:Integrin alpha3beta1; D039161:Integrin alpha6beta4; D007797:Laminin; D020033:Protein Isoforms; D000097794:Kalinin; C119013:laminin 10