[Molecular cloning and homologous modeling of Wulong goose MHC class I gene].
Major histocompatibility complex (MHC) was correlated with immune response for extra-antigen. MHC sequences of chicken, other birds, crawl species and mammalian were derived from GenBank/DDBJ/EMBL and analyzed by alignment, and then primers were designed. By means of LA-PCR method, MHC Classgene was cloned from Wulong goose genomic DNA and total RNA, and the structure of MHC Classgenomic DNA was analyzed using bioinformation methods. The results showed that the genomic DNA, which consisted of 8 extrons and 7 introns, had 64.1% amino acids homologous to chicken and 42.9% amino acids to human. The molecular phylogenetic tree further revealed evolutionary relationship between Wulong goose and other animals such as chicken, other birds, crawl species, mammalian and human. The results of homologous modeling showed that Wulong Goose MHC Classgene was formed by amidocyanogen (N) terminal structure region and carboxyl (C) terminal structure region.
Jia
Xiao-Hui
XH
Institute of High Quality Waterfowl, Laiyang Agricultural University, Qingdao, China.
Wang
Bao-Wei
BW
Wang
Lei
L
Li
Zhen
Z
Zhang
Ming-Ai
MA
Wu
Xiao-Ping
XP
Liu
Guang-Lei
GL
Yang
Zhi-Gang
ZG
Long
Fang-Yu
FY
Zhang
Xu-Hui
XH
D000818:Animals; D003001:Cloning, Molecular; D019295:Computational Biology; D005091:Exons / Q000235:genetics; D005777:Geese / Q000235:genetics*; D005805:Genes, MHC Class I / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics; D008958:Models, Molecular; D010802:Phylogeny; D017433:Protein Structure, Secondary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D015395:Histocompatibility Antigens Class I