Ectopic expression of ThCYP1, a stress-responsive cyclophilin gene from Thellungiella halophila, confers salt tolerance in fission yeast and tobacco cells.
The halophyte Thellungiella halophila (salt cress) is an ideal model system for studying the molecular mechanisms of salinity tolerance in plants. Herein, we report the identification of a stress-responsive cyclophilin gene (ThCYP1) from T. halophila, using fission yeast as a functional system. The expression of ThCYP1 is highly inducible by salt, abscisic acid (ABA), H(2)O(2) and heat shock. Ectopic overexpression of the ThCYP1 gene enhance the salt tolerance capacity of fission yeast and tobacco (Nicotiana tabacum L.) cv. Bright Yellow 2 (BY-2) cells significantly. ThCYP1 is expressed constitutively in roots, stems, leaves and flowers, with higher expression occurring in the roots and flowers. The ThCYP1 proteins are distributed widely within the cell, but are enriched significantly in the nucleus. The present results suggest that ThCYP1 may participate in response to stresses in the salt cress, perhaps by regulating appropriate folding of certain stress-related proteins, or in the signal transduction processes.
Chen
An-Ping
AP
National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Wang
Gui-Ling
GL
Qu
Zhan-Liang
ZL
Lu
Chun-Xia
CX
Liu
Ning
N
Wang
Fang
F
Xia
Gui-Xian
GX
D000040:Abscisic Acid / Q000494:pharmacology; D019607:Brassicaceae / Q000235:genetics* / Q000502:physiology; D021983:Cyclophilins / Q000235:genetics* / Q000502:physiology; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D012568:Schizosaccharomyces / Q000235:genetics* / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D014170:Transformation, Genetic
D018076:DNA, Complementary; D012965:Sodium Chloride; D000040:Abscisic Acid; D021983:Cyclophilins