Systemic and mucosal immunity to respiratory syncytial virus induced by recombinant Streptococcus gordonii surface-displaying a domain of viral glycoprotein G.
A conserved fragment comprising amino acid residues 130-230 of the G glycoprotein of human respiratory syncytial virus subtype A was expressed in the commensal bacterium Streptococcus gordonii. Recombinant streptococci displaying the G domain at the cell surface were used to immunize mice via both parenteral and mucosal routes. Subcutaneous immunization induced respiratory syncytial virus-specific serum immunoglobin G (IgG) capable of partially controlling virus replication in the lungs. Intranasal immunization with live bacteria stimulated the production of IgA against both the whole virus and the G domain in serum and bronchoalveolar fluid. Upon challenge, immunized animals had significantly lower virus titres in the lungs than the controls. Our results show for the first time that the G domain-expressing S. gordonii strain elicits both systemic and mucosal immunity that reduced respiratory syncytial virus replication in the lungs of mice.
Falcone
Valeria
V
Department of Virology, University of Freiburg, Freiburg, Germany. valeria.kapper-falcone@uniklinik-freiburg.de
Mihm
Dagmar
D
Neumann-Haefelin
Dieter
D
Costa
Caterina
C
Nguyen
Thien
T
Pozzi
Gianni
G
Ricci
Susanna
S
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D016503:Drug Delivery Systems; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology*; D018928:Immunity, Mucosal; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000276:immunology*; D010253:Respirovirus Infections / Q000517:prevention & control; D013291:Streptococcus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D015686:Gene Products, env; D008562:Membrane Glycoproteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines