title: Protein sulfation analysis--A primer.
abstract: The aim of this review is to present an overview of protein sulfation in the context of 'modificomics', i.e. post-translational modification-specific proteome research. In addition to a short introduction to the biology of protein sulfation (part 1), we will provide detailed discussion regarding (i) methods and tools for prediction of protein tyrosine sulfation sites (part 2), (ii) biochemical techniques used for protein sulfation analysis (part 3.1), and (iii) mass spectrometric strategies and methods applied to protein sulfation analysis (part 3.2). We will highlight strengths and limitations of different strategies and approaches (including references), providing a primer for newcomers to protein sulfation analysis.
authors.affiliation: Department of Pathology/Enders 1130, Harvard Medical School and Children's Hospital Boston, 320 Longwood Ave, Boston, MA 02115, USA.
authors.forename: Flavio
authors.initials: F
authors.lastname: Monigatti
authors.forename: Brian
authors.initials: B
authors.lastname: Hekking
authors.forename: Hanno
authors.initials: H
authors.lastname: Steen
mesh_terms: D000818:Animals; D004579:Electron Transport; D005169:Factor VIII / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008562:Membrane Glycoproteins / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000378:metabolism*; D019713:Receptors, CCR5 / Q000378:metabolism; D020539:Sequence Analysis, Protein / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015238:Sulfotransferases / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein; D011506:Proteins; D019713:Receptors, CCR5; D014841:von Willebrand Factor; C005662:tyrosine O-sulfate; D014443:Tyrosine; D005169:Factor VIII; D015238:Sulfotransferases; C039079:protein-tyrosine sulfotransferase