Activated human Hageman factor (XII).
An improved method for the isolation of highly purified active human Hageman factor has been described. An overall recovery of 25% with a purification of 10(6) has been achieved. Certain of the physical and chemical characteristics of XII have been elucidated. Selective chemical modification of functional groups and a variety of enzymatic assays have been employed in an effort to shed light on the mechanism of action of this procoagulant.
Speer
R J
RJ
J. K. and Susie L. Wadley Research Institute, Dallas, USA.
Ridgway
H
H
Hill
J M
JM
D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005174:Factor XII / Q000737:chemistry / Q000302:isolation & purification*; D015956:Factor XIIa / Q000737:chemistry / Q000302:isolation & purification*; D005612:Freeze Drying; D006801:Humans; D007525:Isoelectric Focusing
D000596:Amino Acids; D005174:Factor XII; D015956:Factor XIIa