title: Agonist of proteinase-activated receptor 2 increases painful behavior produced by alpha, beta-methylene adenosine 5'-triphosphate.
abstract: Proteinase-activated receptor (PAR) 2 is expressed in a subset of primary afferent neurons and is involved in inflammatory nociception. The P2X3 ion channel is localized on nociceptors of sensory neurons. Using immunohistochemistry, we showed that many P2X3s are co-expressed with the PAR2 in rat dorsal root ganglia neurons. Nocifensive behavior induced by alphabeta-methylene adenosine 5'-triphosphate (ATP) injection to the hind paw was significantly augmented after the application of PAR2 agonists. Fos expression induced by the alphabeta-methylene ATP injection in dorsal horn neurons was also increased after the pre-application of PAR2 agonists. These findings indicate that PAR2 agonists may potentiate the sensitivity of P2X3 ion channel to noxious stimuli, and the interaction between PAR2 and P2X3 may be an important mechanism underlying inflammatory pain.
authors.affiliation: Department of Anatomy and Neuroscience, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
authors.forename: Wan-Jun
authors.initials: WJ
authors.lastname: Zhu
authors.forename: Yi
authors.initials: Y
authors.lastname: Dai
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Fukuoka
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yamanaka
authors.forename: Kimiko
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Koichi
authors.initials: K
authors.lastname: Obata
authors.forename: Shenglan
authors.initials: S
authors.lastname: Wang
authors.forename: Koichi
authors.initials: K
authors.lastname: Noguchi
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000633:toxicity; D000818:Animals; D001522:Behavior, Animal; D004357:Drug Synergism; D015870:Gene Expression / Q000187:drug effects; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D016761:Oncogene Proteins v-fos / Q000378:metabolism; D010146:Pain / Q000139:chemically induced* / Q000503:physiopathology*; D010147:Pain Measurement / Q000379:methods; D020671:Posterior Horn Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044464:Receptor, PAR-2 / Q000819:agonists* / Q000502:physiology; D018048:Receptors, Purinergic P2 / Q000378:metabolism; D058477:Receptors, Purinergic P2X3
chemical_list: D009842:Oligopeptides; D016761:Oncogene Proteins v-fos; C546410:P2rx3 protein, rat; D044464:Receptor, PAR-2; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; C112356:seryl-leucyl-isoleucyl-glycyl--arginyl-leucinamide; D000255:Adenosine Triphosphate; C002630:alpha,beta-methyleneadenosine 5'-triphosphate