title: Is there an epidemiological link between human papillomavirus DNA and basaloid squamous cell carcinoma of the pharynx?
abstract: The epidemiological link between human papillomavirus (HPV) DNA and basaloid squamous cell carcinoma (BSCC) of the pharynx was studied. The expression of p53 protein was evaluated by immunohistochemical analysis. The presence of HPV DNA was evaluated by polymerase chain reaction amplification and "in situ" hybridization. The tobacco and alcohol consumption and the clinical outcomes of nine patients with BSCC of the pharynx are compared with site and stage matched 109 conventional squamous cell carcinoma (SCC) patients. The BSCC specimens were analyzed for the presence of HPV DNA and p53 expression. We did not find any significant differences in tobacco and alcohol consumption between patients with BSCC and patients with SCC. No HPV DNA was detected in BSCC, and p53 overexpression was found in five (55%) of the cases. Our results do not support an etiological link between HPV DNA and BSCC.
authors.affiliation: Departament of Otorhinolaryngology, Hospital Universitario Central de Asturias, and Instituto Universitario de Oncología del Principado de Asturias, Oviedo, Spain.
authors.forename: Rubén
authors.initials: R
authors.lastname: Cabanillas
authors.forename: Juan P
authors.initials: JP
authors.lastname: Rodrigo
authors.forename: Alfio
authors.initials: A
authors.lastname: Ferlito
authors.forename: Alessandra
authors.initials: A
authors.lastname: Rinaldo
authors.forename: Manuel F
authors.initials: MF
authors.lastname: Fresno
authors.forename: Carolina
authors.initials: C
authors.lastname: Aguilar
authors.forename: Marta E
authors.initials: ME
authors.lastname: Alvarez
authors.forename: Carlos
authors.initials: C
authors.lastname: Suárez
mesh_terms: D000368:Aged; D000428:Alcohol Drinking / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology* / Q000378:metabolism / Q000821:virology; D004279:DNA, Viral / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D010610:Pharyngeal Neoplasms / Q000453:epidemiology* / Q000378:metabolism / Q000821:virology; D016133:Polymerase Chain Reaction; D012907:Smoking / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
chemical_list: D004279:DNA, Viral; D016159:Tumor Suppressor Protein p53