Tumoral cell mtDNA approximately 8.9 kb deletion is more common than other deletions in gastric cancer.
BACKGROUND
The aim of the study was to clarify the role of deletion of mitochondrial DNA (mtDNA) in gastric carcinogenesis and to determine prevalence of mitochondrial deletions in different regions of tumoral tissue in comparison with adjacent non-tumoral tissue in gastric cancer.


METHODS
In order to investigate whether a high incidence of mutations exists in mtDNA of gastric cancer tissues, we screened five regions of the mitochondrial genome by PCR amplification, Southern blot and DNA sequence analysis.


RESULTS
Of 71 cancer patients, the approximately 8.9 kb deletion was detected among different deletions in 9 cases (12.67%) of the tumoral tissues and 1 case (1.40%) in non-tumoral tissues that were adjacent to the tumors. Level of the 8.9 kb deletion has been found to be more than other deletions in tumoral tissues.


CONCLUSIONS
The approximately 8.9 kb deletion has an obvious correlation with age and histological type. These data suggest that the approximately 8.9 kb deletion in mtDNA may play an important role in gastric carcinogenesis.
Kamalidehghan
Behnam
B
Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran.
Houshmand
Massoud
M
Panahi
Mehdi Shafa Shariat
MS
Abbaszadegan
Mohammad Reza
MR
Ismail
Patimah
P
Shiroudi
Mahnaz Bagher
MB
D000328:Adult; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011230:Precancerous Conditions / Q000235:genetics*; D017384:Sequence Deletion; D013274:Stomach Neoplasms / Q000235:genetics*
D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm