Proteomic study of plasma from moderate hypercholesterolemic patients.
Proteomics is a technology to detect and identify several proteins and their isoforms in a single sample. We used proteomics to analyze modifications in the protein map of plasma after simvastatin treatment of moderate hypercholesterolemic patients. Plasma from hypercholesterolemic patients (n = 9) was compared before and after 12 weeks of simvastatin treatment (40 mg/day). Patients with similar cardiovascular risk factors were used as controls (CR group). By using two-dimensional electrophoresis and mass spectrometry, we identified the different protein isoforms. The plasma expression of three fibrinogen gamma chain isoforms (FGG) was enhanced, whereas the expression of two isoforms of the fibrinogen beta chain (FGB) was reduced in the hypercholesterolemic patients compared with the CR group. The expression of apolipoprotein A-IV and three haptoglobin isoforms was higher in hypercholesterolemic patients. Simvastatin treatment modified the plasma expression of FGG chain isoform 1, FGB chain isoforms 1 and 2, vitamin D binding protein isoform 3, apo A-IV, and haptoglobin isoform 2. The modification of FGG chain isoform 1 and FGB chain isoforms 1 and 2 was positively correlated with total plasma cholesterol level. Proteomic analysis of plasma may help to know more in depth the molecular mechanism modified by simvastatin treatment.
Alonso-Orgaz
Sergio
S
Cardiovascular Research Unit, Cardiology Department, Cardiovascular Institute, Hospital Clínico San Carlos, Madrid 28040, Spain.
Moreno
Laura
L
Macaya
Carlos
C
Rico
Luis
L
Mateos-Cáceres
Petra J
PJ
Sacristán
Daniel
D
Pérez-Vizcaíno
Francisco
F
Segura
Antonio
A
Tamargo
Juan
J
López-Farré
Antonio
A
D000595:Amino Acid Sequence; D001053:Apolipoproteins / Q000097:blood; D001798:Blood Proteins / Q000032:analysis* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005340:Fibrinogen / Q000032:analysis; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood* / Q000188:drug therapy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D040901:Proteomics / Q000379:methods*; D019821:Simvastatin / Q000494:pharmacology / Q000627:therapeutic use
D001053:Apolipoproteins; D001798:Blood Proteins; D005340:Fibrinogen; D019821:Simvastatin