Simultaneous determination of bisphenol A and its halogenated derivatives in river water by combination of isotope imprinting and liquid chromatography-mass spectrometry.
A restricted access media-molecularly imprinted polymer (RAM-MIP) for [2H16]bisphenol A (BPA-d16) was prepared by a multi-step swelling and polymerization method using 4-vinylpyridine as a functional monomer and ethylene glycol dimethacrylate as a cross-linker, followed by hydrophilic surface modification using glycerol dimethacrylate and glycerol monomethacrylate as hydrophilic monomers. The obtained RAM-MIP showed excellent molecular recognition abilities for BPA and BPA-d6 as well as BPA-d16 used as the template molecule, and good ones for tetrachlorobisphenol A (Cl4-BPA) and tetrabromobisphenol A (Br4-BPA). Next, the RAM-MIP was utilized for selective on-line pretreatment and enrichment of BPA, Cl4-BPA and Br4-BPA in a river water sample, followed by their separation and determination by LC-MS. The calibration graphs of BPA, Cl4-BPA and Br4-BPA, constructed using BPA-d6 as an internal standard, showed good linearity in the range of 12.5-200 pg/mL (r > 0.999) with a 2-mL injection of a river water sample. The inter-day precision data for the assay of BPA, Cl4-BPA and Br4-BPA at 25 pg/mL were 1.08, 3.67 and 1.58%, respectively. Furthermore, this method was successfully applied for the simultaneous determination of BPA and its halogenated derivatives in river water.
Sambe
Haruyo
H
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Koshien Kyuban-cho, Nishinomiya, Hyogo 663-8179, Japan.
Hoshina
Kaori
K
Hosoya
Ken
K
Haginaka
Jun
J
D001559:Benzhydryl Compounds; D001965:Bromides / Q000032:analysis*; D002712:Chlorides / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D007554:Isotopes; D013058:Mass Spectrometry / Q000379:methods*; D010636:Phenols / Q000032:analysis* / Q000737:chemistry*; D045483:Rivers / Q000737:chemistry*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D001965:Bromides; D002712:Chlorides; D007554:Isotopes; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds