Nuclear calcium signals during L-LTP induction do not predict the degree of synaptic potentiation.
The magnitude and/or duration of nuclear Ca(2+)-transients has been shown to dose-dependently modulate gene transcription upon neuronal activation. This is an attractive model for synapse-to-nucleus communication. In order to encode synaptic information, these nuclear Ca(2+)-transients have to be correlated with changes in synaptic strength rather than changes in gene expression patterns. In this study, we analysed nuclear Ca(2+) signals during L-LTP induction. Using a combined approach of fEPSP recordings and two-photon imaging, these Ca(2+) signals were correlated with different degrees of synaptic potentiation in CA1 hippocampal slices. To refine our analysis on the single-cell level, we developed a new approach called single-cell-excitability-probing (SCEP) to assay the plasticity outcome of individual cells by optical means. The degrees of synaptic potentiation we observed could be categorized into transcription independent, transcription-dependent and reduced transcription-dependent. There is no consistent dose-dependent relationship between these different degrees of synaptic potentiation and the magnitude, the decay time and the area under the curve of nuclear Ca(2+)-transients during L-LTP induction. This indicates that nuclear Ca(2+)-transients during induction are unsuited to grade the degree of plasticity in an analogue manner. We propose a role for nuclear Ca(2+) as a digital on/off switch for activating transcription.
Johenning
Friedrich W
FW
Institute of Neuroscience, TU Munich, Biedersteinerstr. 29, 80802 Munich, Germany. friedrich.johenning@charite.de <friedrich.johenning@charite.de>
Holthoff
Knut
K
D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D017774:Long-Term Potentiation / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D017966:Pyramidal Cells / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology
D002118:Calcium