Structure of the mitochondrial DNA control region of the sinipercine fishes and their phylogenetic relationship.
The mitochondrial DNA control region of Siniperca chuatsi, S. kneri, S. scherzeri, S. obscura, S. undulata, Coreosiniperca roulei and Coreoperca whiteheadi were amplified by PCR amplification and directly sequenced. The mtDNA control region of the sinipercine fishes could be separated into three domains, namely, the terminal associated sequence domain, the central conserved sequence domain and the conserved sequence block domain. The extended terminal associated sequence (ETAS), three conserved sequence blocks (CSB-F, CSB-E, CSB-D) in the central conserved sequence domain and three conserved sequence blocks (CSB1, CSB2, CSB3) in the conserved sequence block domain were also identified. The phylogenetic relationships among these sinipercine fishes were constructed through neighbor-joining and maximum parsimony methods using Percidae and Serranidae as outgroups. Results showed that sinipercine fishes were a monophyletic group, with Siniperca forming one group, and Coreoperca forming another group. Coreosiniperca roulei did not form an independent group but was merged into the genus Siniperca. Thus it should be renamed as Siniperca roulei.
Zhao
Jin-Liang
JL
Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem Certificated by the Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090, China. jlzhao@shfu.edu.cn
Wang
Wei-Wei
WW
Li
Si-Fa
SF
Cai
Wan-Qi
WQ
D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D005399:Fishes / Q000145:classification / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D016415:Sequence Alignment
D004272:DNA, Mitochondrial