Human serum albumin supported lipid patterns for the targeted recognition of microspheres coated by membrane based on ss-DNA hybridization.
Human serum albumin (HSA) patterns have been successfully fabricated for the deposition of lipid bilayer, 1,2-dimyristoyl-sglycerophosphate (DMPA), by making use of the micro-contact printing (microCP) technique and liposome fusion. Confocal laser scanning microscopy (CLSM) results indicate that lipid bilayer has been assembled in HSA patterns with a good stability. Such well-defined lipid patterns formed on HSA surface create possibility to incorporate specific components like channels or receptors for specific recognition. In view of this, microspheres coated with lipid membranes were immobilized in HSA-supported lipid patterns via the hybridization of complementary ss-DNAs. This procedure enables to transfer solid materials to a soft surface through a specific recognition.
Zhang
Xiaoming
X
Beijing National Lab for Molecular Sciences, Key Lab of Colloid and Interface Science, The Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
He
Qiang
Q
Cui
Yue
Y
Duan
Li
L
Li
Junbai
J
D004277:DNA, Single-Stranded / Q000235:genetics*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008567:Membranes, Artificial; D008863:Microspheres; D008958:Models, Molecular; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization; D012709:Serum Albumin / Q000737:chemistry*
D004277:DNA, Single-Stranded; D008055:Lipids; D008567:Membranes, Artificial; D012709:Serum Albumin