title: RNA-protein interactions at the initial and terminal stages of protein biosynthesis as investigated by Lev Kisselev (on the occasion of his 70th anniversary).
abstract: This review highlights studies by Lev L. Kisselev and his colleagues on the initial and terminal stages of protein biosynthesis, which cover the period of the last 45 years (1961-2006). They investigated spatial structure of tRNAs, structure and functions of aminoacyl-tRNA-synthetases of higher organisms, and the final step of protein synthesis, termination of translation. L. Kisselev and his team have made three major contributions to these fields of molecular biology; (i) they proposed the hypothesis on the role of anticodon triplet of tRNA in recognition by cognate aminoacyl-tRNA synthetase, which has been experimentally confirmed and is now included in textbooks; (ii) identified primary structures and functions of two eukaryotic protein factors (eRF1 and eRF3) playing a pivotal role in translation termination; (iii) characterized a structural basis for stop codon recognition by eRF1 within the ribosome and discovered the negative structural elements of eRF1, limiting its recognition of one or two stop-codons.
authors.affiliation: Lomonosov Moscow State University, Moscow, 119992, Russia. bogdanov@belozersky.msu.ru
authors.forename: A A
authors.initials: AA
authors.lastname: Bogdanov
authors.forename: V L
authors.initials: VL
authors.lastname: Karpov
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000266:history / Q000378:metabolism; D049673:History, 20th Century; D049674:History, 21st Century; D010442:Peptide Chain Initiation, Translational; D010443:Peptide Chain Termination, Translational; D010454:Peptide Termination Factors / Q000266:history / Q000378:metabolism; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000235:genetics / Q000266:history / Q000378:metabolism
chemical_list: D010454:Peptide Termination Factors; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases