High-performance liquid chromatography analysis of rubber allergens in protective gloves used in health care.
A high-performance liquid chromatography (HPLC) method developed for analysis of zinc dithiocarbamates was validated and used to perform a survey of disposable medical gloves used in southern Sweden. The gloves were extracted with acetone at room temperature for 10 min by shaking. The extracts were injected into a polyether ether ketone lined HPLC column, and peaks were analysed by a diode-array detector. The survey shows that of 19 gloves analysed, 10 contained zinc diethyldithiocarbamate (0.070-3.5 mg/g), 3 contained zinc pentamethylenedithiocarbamate (1.0-4.3 mg/g), 4 contained zinc dibutyldithiocarbamate (0.9-1.1 mg/g), and 2 contained 2-mercaptobenzothiazole (0.005-0.008 mg/g). None of them contained thiurams.
Bergendorff
Ola
O
Department of Dermatology, Lund University, University Hospital, SE-221 85 Lund, Sweden. ola.bergendorff@skane.se
Persson
C
C
Hansson
C
C
D000485:Allergens / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009783:Dermatitis, Occupational / Q000517:prevention & control*; D017575:Gloves, Protective; D006229:Hand Dermatoses / Q000517:prevention & control*; D006282:Health Personnel; D006801:Humans; D020315:Latex Hypersensitivity / Q000517:prevention & control*; D011237:Predictive Value of Tests; D012408:Rubber / Q000737:chemistry*; D013859:Thiocarbamates / Q000737:chemistry
D000485:Allergens; D013859:Thiocarbamates; D012408:Rubber; C017801:zinc dibutyldithiocarbamate