title: Comparison of API 20E and invA PCR for identification of Salmonella enterica isolates from swine production units.
abstract: API 20E and invA PCR were evaluated for the identification of Salmonella enterica isolates from swine farms. API 20E had the highest agreement with other tests at the 99.9% likelihood level. Both tests had 100% sensitivity and 96% specificity compared to 16S rRNA sequencing. Compared to serotyping, both tests had 96% sensitivity; specificity was 86% for API 20E and 79% for invA PCR.
authors.affiliation: Department of Pathobiology, University of Illinois, 2001 South Lincoln Avenue, Urbana, 61802, USA.
authors.forename: Daniele M
authors.initials: DM
authors.lastname: Nucera
authors.forename: Carol W
authors.initials: CW
authors.lastname: Maddox
authors.forename: Patricia
authors.initials: P
authors.lastname: Hoien-Dalen
authors.forename: Ronald M
authors.initials: RM
authors.lastname: Weigel
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods; D001431:Bacteriological Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; C075518:invA protein, Bacteria