Distribution of calbindin-28kD and parvalbumin in V1 in normal adult Cebus apella monkeys and in monkeys with retinal lesions.
Several proteins have their normal patterns of distributions altered by monocular visual deprivation. We studied the distribution of the calcium-binding proteins calbindin-28kD (Cb) and parvalbumin (Pv) in V1 in normal adult Cebus apella monkeys and in monkeys with monocular retinal lesions. In normal monkeys, the interblobs regions in layers 2/3 and the layer 4B are intensely labeled for Cb, while Pv reaction showed a complementary labeling pattern with a stronger staining in layers 4A, 4C and in the blob regions in layers 2/3. In monkeys with monocular retinal lesion, the laminar distribution of these proteins was differentially affected, although both reactions resulted in stronger labeling in non-deprived ocular dominance columns. While Cb reaction resulted in stronger labeling in layers 1 through 5, Pv labeling was heavier in layers 2/3, 4A and 4C. There was a clear reduction in the intensity of neuropil staining for both Pv and Cb in deprived ocular dominance columns with little or no reduction in number of labeled cells. This reduction could thus be attributed to activity-dependent changes at synapses level.
Pinheiro Botelho
Eliã
E
Laboratório Fisiologia da Cognição, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
Guimarães Martins Soares
Juliana
J
da Silva Pereira
Sandra
S
Fiorani
Mario
M
Gattass
Ricardo
R
D000818:Animals; D064026:Calbindins; D002428:Cebus / Q000033:anatomy & histology / Q000502:physiology*; D004195:Disease Models, Animal; D023882:Dominance, Ocular / Q000502:physiology; D003576:Electron Transport Complex IV / Q000378:metabolism; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D019581:Neuropil / Q000378:metabolism / Q000648:ultrastructure; D010320:Parvalbumins / Q000378:metabolism*; D010802:Phylogeny; D012164:Retinal Diseases / Q000378:metabolism* / Q000503:physiopathology; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013045:Species Specificity; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure; D014786:Vision Disorders / Q000378:metabolism* / Q000503:physiopathology; D014793:Visual Cortex / Q000166:cytology / Q000378:metabolism*; D014795:Visual Pathways / Q000378:metabolism* / Q000503:physiopathology
D064026:Calbindins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; D003576:Electron Transport Complex IV