title: Ionizable isotopic labeling reagent for relative quantification of amine metabolites by mass spectrometry.
abstract: A powerful approach to relative quantification by mass spectrometry is to employ labeling reagents that target specific functional groups in molecules of interest. A quantitative comparison of two or more samples may be readily accomplished by using a chemically identical but isotopically distinct labeling reagent for each sample. The samples may then be combined, subjected to purification steps, and mass analyzed. Comparison of the signal intensities obtained from the isotopically labeled variants of the target analyte(s) provides quantitative information on their relative concentrations in the sample. In this report, we describe the synthesis and use of heavy and light isotopic forms of methyl acetimidate for the relative quantification of amine-containing species. The principal advantages of methyl acetimidate as a labeling reagent are that the reaction product is positively charged and hydrophobicity is increased, both of which enhance electrospray ionization efficiency and increase detection sensitivity. The quantitative nature of the analysis was demonstrated in model metabolomics experiments in which heavy and light labeled Arabidopsis extracts were combined in different ratios. Finally, the labeling strategy was employed to determine differences in the amounts of amine-containing metabolites for Arabidopsis seeds germinated under two different conditions.
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Shortreed
authors.forename: Shane M
authors.initials: SM
authors.lastname: Lamos
authors.forename: Brian L
authors.initials: BL
authors.lastname: Frey
authors.forename: Margaret F
authors.initials: MF
authors.lastname: Phillips
authors.forename: Madhusudan
authors.initials: M
authors.lastname: Patel
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Belshaw
authors.forename: Lloyd M
authors.initials: LM
authors.lastname: Smith
mesh_terms: D000588:Amines / Q000032:analysis / Q000737:chemistry*; D000596:Amino Acids / Q000032:analysis; D017360:Arabidopsis / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002853:Chromatography, Liquid; D007096:Imidoesters / Q000138:chemical synthesis / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods*; D012639:Seeds / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000588:Amines; D000596:Amino Acids; D029681:Arabidopsis Proteins; D007096:Imidoesters; C010222:methyl acetimidate