Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases.
We cloned lipG, which encoded a lipolytic enzyme, from a Korean tidal flat metagenomic library. LipG was related to six putative lipases previously identified only in bacterial genome sequences. These enzymes comprise a new family. We partially characterized LipG, providing the first experimental data for a member of this family.
Lee
Mi-Hwa
MH
Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
Lee
Choong-Hwan
CH
Oh
Tae-Kwang
TK
Song
Jae Kwang
JK
Yoon
Jung-Hoon
JH
D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004952:Esters / Q000378:metabolism; D015698:Genomic Library; D019015:Geologic Sediments / Q000737:chemistry* / Q000382:microbiology; D007723:Korea; D008049:Lipase / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009574:Nitro Compounds / Q000378:metabolism; D010802:Phylogeny; D012623:Seawater / Q000737:chemistry* / Q000382:microbiology
D001426:Bacterial Proteins; D004952:Esters; D009574:Nitro Compounds; D008049:Lipase