Enzymatic synthesis of L-pipecolic acid by Delta1-piperideine-2-carboxylate reductase from Pseudomonas putida.
L-Pipecolic acid is a chiral pharmaceutical intermediate. An enzymatic system for the synthesis of L-pipecolic acid from L-lysine by commercial L-lysine alpha-oxidase from Trichoderma viride and an extract of recombinant Escherichia coli cells coexpressing Delta1-piperideine-2-carboxylate reductase from Pseudomonas putida and glucose dehydrogenase from Bacillus subtilis is described. A laboratory-scale process provided 27 g/l of L-pipecolic acid in 99.7% e.e.
Muramatsu
Hisashi
H
Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan.
Mihara
Hisaaki
H
Yasuda
Mari
M
Ueda
Makoto
M
Kurihara
Tatsuo
T
Esaki
Nobuyoshi
N
D001412:Bacillus subtilis / Q000201:enzymology; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D010875:Pipecolic Acids / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology
D010875:Pipecolic Acids; D006899:Mixed Function Oxygenases; D042843:Glucose 1-Dehydrogenase; C100031:lysine monooxygenase; D000587:Oxidoreductases Acting on CH-NH Group Donors; C032772:1,2-didehydropipecolate reductase; C031345:pipecolic acid