Peripheral blood B-cell death compensates for excessive proliferation in lymphoid tissues and maintains homeostasis in bovine leukemia virus-infected sheep.
The size of a lymphocyte population is primarily determined by a dynamic equilibrium between cell proliferation and death. Hence, lymphocyte recirculation between the peripheral blood and lymphoid tissues is a key determinant in the maintenance of cell homeostasis. Insights into these mechanisms can be gathered from large-animal models, where lymphatic cannulation from individual lymph nodes is possible. In this study, we assessed in vivo lymphocyte trafficking in bovine leukemia virus (BLV)-infected sheep. With a carboxyfluorescein diacetate succinimidyl ester labeling technique, we demonstrate that the dynamics of lymphocyte recirculation is unaltered but that accelerated proliferation in the lymphoid tissues is compensated for by increased death in the peripheral blood cell population. Lymphocyte homeostasis is thus maintained by biphasic kinetics in two distinct tissues, emphasizing a very dynamic process during BLV infection.
Debacq
Christophe
C
Molecular and Cellular Biology, FNRS-FUSAG, Gembloux, Belgium.
Gillet
Nicolas
N
Asquith
Becca
B
Sanchez-Alcaraz
Maria Teresa
MT
Florins
Arnaud
A
Boxus
Mathieu
M
Schwartz-Cornil
Isabelle
I
Bonneau
Michel
M
Jean
Geneviève
G
Kerkhofs
Pierre
P
Hay
Jack
J
Théwis
André
A
Kettmann
Richard
R
Willems
Luc
L
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D039481:CD11b Antigen / Q000378:metabolism; D016923:Cell Death; D002465:Cell Movement; D049109:Cell Proliferation; D006800:Deltaretrovirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D005452:Fluoresceins; D006706:Homeostasis; D007700:Kinetics; D001909:Leukemia Virus, Bovine / Q000276:immunology / Q000502:physiology*; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology* / Q000473:pathology / Q000821:virology*; D012756:Sheep / Q000276:immunology / Q000821:virology; D012757:Sheep Diseases / Q000276:immunology / Q000821:virology; D013388:Succinimides
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D039481:CD11b Antigen; D005452:Fluoresceins; D013388:Succinimides