title: Glaciecola nitratireducens sp. nov., isolated from seawater.
abstract: A marine bacterial strain, FR1064(T), was isolated from a surface seawater sample collected off Jeju Island, South Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the Gammaproteobacteria and was related to the genus Glaciecola with 97.6 % sequence similarity to Glaciecola pallidula, its nearest phylogenetic neighbour. DNA-DNA relatedness between strain FR1064(T) and G. pallidula ACAM 615(T) was 55 %. Cells of the novel isolate were Gram-negative, aerobic, rod-shaped, motile and halophilic, with an optimum sea salts concentration of 4-7 %. The major fatty acids were straight-chain saturated (C(16 : 0)), summed feature 3 and monounsaturated fatty acid C(18 : 1). The DNA G+C content was 44 mol%. Several phenotypic characteristics differentiated the novel isolate from all previously described members of the genus Glaciecola. The polyphasic data obtained in this study clearly demonstrate that strain FR1064(T) represents a novel species of the genus Glaciecola. The name Glaciecola nitratireducens sp. nov. is therefore proposed, with strain FR1064(T) (=KCTC 12276(T)=JCM 12485(T)) as the type strain.
authors.affiliation: Department of Biology, College of Natural Sciences, Sunchon National University, Sunchon 540-742, Republic of Korea.
authors.forename: Keun Sik
authors.initials: KS
authors.lastname: Baik
authors.affiliation: School of Biological Sciences and Institute of Microbiology, Seoul National University, NS70, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Yoon-Dong
authors.initials: YD
authors.lastname: Park
authors.affiliation: Department of Biology, College of Natural Sciences, Sunchon National University, Sunchon 540-742, Republic of Korea.
authors.forename: Chi Nam
authors.initials: CN
authors.lastname: Seong
authors.affiliation: Department of Dental Hygiene, Kwangyang Health College, Kwangyang 545-703, Republic of Korea.|Department of Biology, College of Natural Sciences, Sunchon National University, Sunchon 540-742, Republic of Korea.
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Kim
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Yusung PO Box 115, Taejon 305-600, Republic of Korea.
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Bae
authors.affiliation: School of Biological Sciences and Institute of Microbiology, Seoul National University, NS70, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Jongsik
authors.initials: J
authors.lastname: Chun
mesh_terms: D034241:Alteromonadaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D012623:Seawater / Q000382:microbiology*; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S