title: Immunostimulation effect of jellyfish collagen.
abstract: Certain edible large jellyfishes belonging to the order Rhizostomeae are consumed in large quantities in China and Japan. The exumbrella part of the edible jellyfish Stomolophus nomurai was cut and soaked in dilute hydrochloric acid solution (pH 3.0) for 12 h, and heated at 121 degrees C for 20 min. The immunostimulation effects of the jellyfish extract were examined. The jellyfish extract enhanced IgM production of human hybridoma HB4C5 cells 34-fold. IgM and IgG production of human peripheral blood lymphocytes (PBL) were also accelerated, 2.8- and 1.4-fold respectively. Moreover, production of interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha by human PBL was stimulated 100- and 17-fold respectively. Collagenase treatment inactivated the immunostimulation activity of the jellyfish extract. In addition, purified collagen from bovine Achilles' tendon accelerated IgM production of hybridoma cells. These facts mean that collagen has an immunostimulation effect, and that the active substance in jellyfish extract is collagen.
authors.affiliation: Faculty of Agriculture, Ehime University, Matsuyama, Ehime, Japan. mars95@agr.ehime-u.ac.jp
authors.forename: Takuya
authors.initials: T
authors.lastname: Sugahara
authors.forename: Masashi
authors.initials: M
authors.lastname: Ueno
authors.forename: Yoko
authors.initials: Y
authors.lastname: Goto
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Shiraishi
authors.forename: Mikiharu
authors.initials: M
authors.lastname: Doi
authors.forename: Koichi
authors.initials: K
authors.lastname: Akiyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamauchi
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D003063:Cnidaria / Q000276:immunology*; D003094:Collagen / Q000276:immunology* / Q000302:isolation & purification; D017364:Collagenases / Q000378:metabolism / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D009504:Neutrophils; D037401:Scyphozoa / Q000737:chemistry / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D003094:Collagen; D017364:Collagenases