Mutations of connexin 26 at position 75 and dominant deafness: essential role of arginine for the generation of functional gap-junctional channels.
Gap-junctional channels are large intercellular aqueous pores formed by head-to-head association of two gap-junctional hemichannels (connexin hexamers), one from each of the adjacent cells. The mechano-transduction of sound waves into electrical impulses occurs in the cochlea, which houses the organ of Corti. Hereditary deafness is frequent and mutations of connexin 26, the predominant connexin of the cochlea, are its most frequent cause. Mutations of R75 cause deafness and disrupt gap-junctional communication. Here, we determined the effects of substitutions of R75 with different residues (alanine, asparagine, aspartic acid, lysine, phenylalanine, tyrosine or tryptophan) on formation of gap-junctional channels and hemichannels. We show that connexin 26 R75 is essential for the formation of gap-junctional channels. Substitution of R75 with aromatic residues yields functional hemichannels that display altered voltage dependence, whereas substitution with other residues yields non-functional hemichannels. The expression of R75 mutants has a dominant negative effect on gap-junctional communication mediated by wild-type connexin 26, independently of the ability of the mutants to form functional gap-junctional hemichannels. Our results show that the arginine located at position 75 of connexin 26 is essential for function, and cannot be replaced by other residues.
Deng
Yanqin
Y
Department of Neuroscience and Cell Biology, and the Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical Branch, Galveston, TX 77555-0437, United States.
Chen
Yongyue
Y
Reuss
Luis
L
Altenberg
Guillermo A
GA
D024322:Amino Acids, Aromatic / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D001120:Arginine / Q000235:genetics / Q000502:physiology*; D017630:Connexins / Q000737:chemistry / Q000235:genetics*; D003638:Deafness / Q000235:genetics*; D017629:Gap Junctions / Q000502:physiology*; D015640:Ion Channel Gating; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009865:Oocytes / Q000378:metabolism; D014982:Xenopus laevis
D024322:Amino Acids, Aromatic; D017630:Connexins; D001120:Arginine