Construction of a deletion strain and expression vector for the Escherichia coli NADH:ubiquinone oxidoreductase (Complex I).
Complex I of Escherichia coli is encoded by 13 consecutive genes, called the nuo operon. A chromosomal deletion of all nuo genes has been achieved by homologous recombination. A vector that encodes all of the nuo genes has been constructed, and it expresses a functional enzyme.
Amarneh
Bilal
B
Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275-0376, USA.
De Leon-Rangel
Jessica
J
Vik
Steven B
SB
D042967:Electron Transport Complex I / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D017353:Gene Deletion; D015870:Gene Expression; D005798:Genes, Bacterial; D005822:Genetic Vectors; D005810:Multigene Family; D009876:Operon; D010957:Plasmids / Q000235:genetics
D042967:Electron Transport Complex I