title: Molecular characterization of the largest mycoviral-like double-stranded RNAs associated with Amasya cherry disease, a disease of presumed fungal aetiology.
abstract: The sequence of the four large (L) double-stranded RNAs (dsRNAs) associated with Amasya cherry disease (ACD), which has a presumed fungal aetiology, is reported. ACD L dsRNAs 1 (5121 bp) and 2 (5047 bp) potentially encode proteins of 1628 and 1620 aa, respectively, that are 37% identical and of unknown function. ACD L dsRNAs 3 (4458 bp) and 4 (4303 bp) potentially encode proteins that are 68% identical and contain the eight motifs conserved in RNA-dependent RNA polymerases (RdRp) of dsRNA mycoviruses, having highest similarity with those of members of the family Totiviridae. Both terminal regions share extensive conservation in all four RNAs, suggesting a functional relationship between them. As ACD L dsRNAs 1 and 2 do not encode RdRps, both are probably replicated by those from either ACD L dsRNA 3 or 4. Partial characterization of the equivalent L dsRNAs 3 and 4 associated with cherry chlorotic rusty spot revealed essentially identical sequences.
authors.affiliation: Division of Biology, Faculty of Natural Sciences, Sir Alexander Fleming Building, Imperial College London, Imperial College Road, London SW7 2AZ, UK.
authors.forename: Z
authors.initials: Z
authors.lastname: Kozlakidis
authors.affiliation: Dipartimento di Arboricoltura, Botanica e Patologia Vegetale, Università di Napoli, 80055 Portici, Italy.|Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, 46022 Valencia, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Covelli
authors.affiliation: Istituto di Virologia Vegetale del CNR, Sezione di Bari, 70126 Bari, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Di Serio
authors.affiliation: Tekirdag Ziraat Fakültesi, Trakya Universitesi, 59030 Tekirdag, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Citir
authors.affiliation: Adnan Menderes University, Agricultural Faculty, Plant Pathology Department, 09100 Aydin, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Açıkgöz
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, 46022 Valencia, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Hernández
authors.affiliation: Dipartimento di Arboricoltura, Botanica e Patologia Vegetale, Università di Napoli, 80055 Portici, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Ragozzino
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, 46022 Valencia, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Flores
authors.affiliation: Division of Biology, Faculty of Natural Sciences, Sir Alexander Fleming Building, Imperial College London, Imperial College Road, London SW7 2AZ, UK.
authors.forename: R H A
authors.initials: RHA
authors.lastname: Coutts
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005658:Fungi / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D027861:Prunus; D012330:RNA, Double-Stranded / Q000032:analysis* / Q000235:genetics* / Q000302:isolation & purification; D019196:Totiviridae / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012330:RNA, Double-Stranded; D014764:Viral Proteins