title: SLRP interaction can protect collagen fibrils from cleavage by collagenases.
abstract: Decorin, fibromodulin and lumican are small leucine-rich repeat proteoglycans (SLRPs) which interact with the surface of collagen fibrils. Together with other molecules they form a coat on the fibril surface which could impede the access to collagenolytic proteinases. To address this hypothesis, fibrils of type I or type II collagen were formed in vitro and treated with either collagenase-1 (MMP1) or collagenase-3 (MMP13). The fibrils were either treated directly or following incubation in the presence of the recombinant SLRPs. The susceptibility of the uncoated and SLRP-coated fibrils to collagenase cleavage was assessed by SDS/PAGE. Interaction with either recombinant decorin, fibromodulin or lumican results in decreased collagenase cleavage of both fibril types. Thus SLRP interaction can help protect collagen fibrils from cleavage by collagenases.
authors.affiliation: Genetics Unit, Shriners Hospital for Children, 1529 Cedar Avenue, Montreal, Quebec, Canada H3G 1A6.
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Geng
authors.forename: David
authors.initials: D
authors.lastname: McQuillan
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Roughley
mesh_terms: D000818:Animals; D002417:Cattle; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D017364:Collagenases / Q000737:chemistry* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016326:Extracellular Matrix Proteins / Q000737:chemistry; D000071235:Fibromodulin; D006801:Humans; D007632:Keratan Sulfate / Q000737:chemistry; D007930:Leucine / Q000737:chemistry*; D000071234:Lumican; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011509:Proteoglycans / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D016326:Extracellular Matrix Proteins; C000604330:FMOD protein, human; C000604306:LUM protein, human; D000071234:Lumican; D011509:Proteoglycans; D011994:Recombinant Proteins; D000071235:Fibromodulin; D003094:Collagen; D007632:Keratan Sulfate; D017364:Collagenases; D053509:Matrix Metalloproteinase 13; D020781:Matrix Metalloproteinase 1; D007930:Leucine