Phenolics and antimicrobial activity of traditional stoned table olives 'alcaparra'.
In the present work, we report the determination of phenolic compounds in 'alcaparra' table olives by reversed-phase HPLC/DAD, and the evaluation of their extract in vitro activity against several microorganisms that may be causal agents of human intestinal and respiratory tract infections, namely Gram positive (Bacillus cereus, Bacillus subtilis, and Staphylococcus aureus), Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae) and fungi (Candida albicans and Cryptococcus neoformans). Three flavonoidic compounds were identified and quantified: luteolin 7-O-glucoside, apigenin 7-O-glucoside, and luteolin. At low concentrations (0.05mg/mL) 'alcaparra' extract revealed significant inhibition of both Gram positive and Gram negative bacteria growth, with exception of P. aeruginosa. Nevertheless, no antifungal activity was observed at the tested concentrations.
Sousa
Anabela
A
CIMO/Escola Superior Agrária, Instituto Politécnico de Bragança, Campus Sta Apolónia, Apartado 1172, 5301-855 Bragança, Portugal.
Ferreira
Isabel C F R
IC
Calhelha
Ricardo
R
Andrade
Paula B
PB
Valentão
Patrícia
P
Seabra
Rosa
R
Estevinho
Letícia
L
Bento
Albino
A
Pereira
José Alberto
JA
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005658:Fungi / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D031658:Olea / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D011174:Portugal
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010636:Phenols; D010936:Plant Extracts