Free-energy landscape of alcohol driven coacervation transition in aqueous gelatin solutions.
Liquid-liquid phase separation of a homogeneous polyampholyte (gelatin) solution into a dense polymer-rich coacervate and the dilute supernatant phase is discussed through free-energy landscape formalism. We have evaluated the free energy and entropy of the system as it undergoes the phenomenon of simple coacervation, driven by the addition of a nonsolvent. Electrophoretic mobility (mu) and turbidity measurements were performed on 0.01% and 0.05% (w/v) aqueous gelatin solutions that were driven towards coacervation by the addition of ethanol. The mobility of the polyampholyte molecules, which was typically mu approximately 0.38+/-0.02 microm/s cm/V in water, gradually reduced for the soluble intermolecular complexes to a plateau value of mu approximately 0.11+/-0.01 microm/s cm/V as the ethanol volume fraction equaled phi(ns) approximately 0.47+/-0.03, which coincided with the first appearance of coacervate droplets (coacervation transition) observed from turbidity measurements, a behavior found to be invariant of gelatin concentration. These results were used as input to the theoretical model to explicitly construct the free-energy landscape for a single gelatin chain and the global system comprising the polymer-rich coacervate and the dilute supernatant phase.
Reena
Amarnath Gupta
AG
Polymer and Biophysics Laboratory, School of Physical Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Bohidar
H B
HB