Dynamic seeding and perfusion culture of hepatocytes with galactosylated vegetable sponge in packed-bed bioreactor.
A galactose moiety was introduced into the fiber surface of a vegetable sponge by the covalent binding of lactobionic acid. The galactosylated sponge was used as scaffold for the culture of rat hepatocytes in a packed-bed bioreactor. Hepatocytes could be dynamically seeded into and uniformly distributed throughout the scaffold, and the immobilized cells maintained high albumin and urea production rates during long-term perfusion culture. The hepatocytes showed an increasing albumin production rate from 49 to 109 microg/10(6) cells/d over the 7-d culture.
Chen
Jyh-Ping
JP
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Kwei-San, Taoyuan 333, Taiwan, ROC. jpchen@mail.cgu.edu.tw
Lin
Chia-Tseng
CT
D000418:Albumins / Q000378:metabolism; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D049109:Cell Proliferation; D002470:Cell Survival; D018914:Cells, Immobilized / Q000502:physiology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005638:Fruit / Q000737:chemistry; D005690:Galactose / Q000737:chemistry / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology*; D019164:Liver, Artificial; D029776:Luffa / Q000737:chemistry*; D008422:Materials Testing; D010477:Perfusion / Q000295:instrumentation / Q000379:methods
D000418:Albumins; D001672:Biocompatible Materials; D005690:Galactose