title: Demonstration of receptor binding properties of VP2 of very virulent strain infectious bursal disease virus on Vero cells.
abstract: Tissue culture adaptation of infectious bursal disease virus (IBDV) results in alternation of three residues on its major capsid protein VP2 and these residues may engage in receptor binding. Although the key of successful infection of tissue culture adapted IBDV in tissue cultures was defined as the virus entering steps, mechanism of the adaptation is poorly understood. In this study, recombinant VP2s of an attenuated strain (D78) and a very virulent strain (HK46) of IBDV tagged with rabbit immunoglobulin G heavy chain were expressed in mammalian cells, generating RAVP2 and RVVP2, respectively, in high purity. Using flow cytometry, both RAVP2 and RVVP2 were demonstrated to bind with Vero cells while these bindings were blocked by D78 viral particles, implying both very virulent IBDVs (vvIBDVs) and attenuated IBDVs bind to Vero cells through the same receptor(s). Since vvIBDVs cannot be propagated directly in tissue cultures, the specific binding between RVVP2 and Vero cells suggests the barrier for tissue culture adaptation may be beyond the virus attachment process.
authors.affiliation: Department of Zoology, University of Hong Kong, Pokfulam Road, Hong Kong SAR, China.
authors.forename: Chi Wai
authors.initials: CW
authors.lastname: Yip
authors.forename: Yin Shan
authors.initials: YS
authors.lastname: Yeung
authors.forename: Ching Man
authors.initials: CM
authors.lastname: Ma
authors.forename: Pui Yi
authors.initials: PY
authors.lastname: Lam
authors.forename: Chung Chau
authors.initials: CC
authors.lastname: Hon
authors.forename: Fanya
authors.initials: F
authors.lastname: Zeng
authors.forename: Frederick Chi Ching
authors.initials: FC
authors.lastname: Leung
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D018175:Birnaviridae Infections / Q000821:virology*; D036022:Capsid Proteins / Q000378:metabolism*; D002522:Chlorocebus aethiops; D007243:Infectious bursal disease virus / Q000737:chemistry* / Q000502:physiology*; D011991:Receptors, Virus / Q000378:metabolism*; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins; D011991:Receptors, Virus