Aquaporin NIP2;1 is mainly localized to the ER membrane and shows root-specific accumulation in Arabidopsis thaliana.
We investigated a nodulin 26-like protein NIP2;1, which belongs to the third subgroup of Arabidopsis aquaporins. Histochemical analysis of a promoter-beta-glucuronidase fusion revealed the root-specific expression of NIP2;1. The NIP2;1 protein was detected in young roots, but not in leaves, stems, flowers or siliques. The transient expression of NIP2;1 linked with green fluorescent protein in Arabidopsis cultured cells showed its putative endoplasmic reticulum (ER) localization. NIP2;1 expressed in yeast cells had low water channel activity in the membranes. NIP2;1 may function as a water channel and/or ER channel for other small molecules or ions.
Mizutani
Masahiro
M
Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan.
Watanabe
Satoshi
S
Nakagawa
Tsuyoshi
T
Maeshima
Masayoshi
M
D000595:Amino Acid Sequence; D020346:Aquaporins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000378:metabolism*; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005966:Glucuronidase; D049452:Green Fluorescent Proteins; D008969:Molecular Sequence Data; D018517:Plant Roots; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014867:Water / Q000378:metabolism
D020346:Aquaporins; D029681:Arabidopsis Proteins; C521613:NIP2;1 protein, Arabidopsis; D014867:Water; D049452:Green Fluorescent Proteins; D005966:Glucuronidase