Extraction, detection, and quantification of flavano-ellagitannins and ethylvescalagin in a Bordeaux red wine aged in oak barrels.
An extraction procedure and an analytical method have been developed to detect and quantify for the first time a series of ellagitannin derivatives formed in wine during aging in oak barrels. The method involves a preliminary purification step on XAD7 HP resin followed by a second purification step on TSK 40 HW gel. The resulting extract is analyzed for compound identification and quantitative determination by high-performance liquid chromatography-electrospray ionization-mass spectrometry in single ion recording mode. Reference compounds, which are accessible through hemisynthesis from the oak C-glycosidic ellagitannin vescalagin, were used to build calibration curves, and chlorogenic acid was selected as an internal standard. This method enabled us to estimate the content of four flavano-ellagitannins and that of another newly identified wine polyphenol, beta-1-O-ethylvescalagin, in a Bordeaux red wine aged for 18 months in oak barrels. All five ellagitannin derivatives are derived from the nucleophilic substitution reaction of vescalagin with the grape flavan-3-ols catechin and epicatechin or ethanol.
Saucier
Cedric
C
Faculté d'Oenologie, Université Victor Segalen Bordeaux 2, UMR 1219 INRA, ISVV, 351 Cours de la Libération, 33405 Talence Cedex, France. saucier@oenologie.u-bordeaux2.fr
Jourdes
Michael
M
Glories
Yves
Y
Quideau
Stephane
S
D001713:Biphenyl Compounds / Q000032:analysis* / Q000737:chemistry; D002392:Catechin / Q000737:chemistry; D002396:Catechols / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D005511:Food Handling / Q000379:methods*; D047348:Hydrolyzable Tannins / Q000032:analysis* / Q000737:chemistry; D029963:Quercus; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors; D014920:Wine / Q000032:analysis*; D014934:Wood
D001713:Biphenyl Compounds; D002396:Catechols; D047348:Hydrolyzable Tannins; C084709:vescalagin; D000431:Ethanol; D002392:Catechin