title: Physiologic roles of 11beta-hydroxysteroid dehydrogenase type 2 in kidney.
abstract: We developed enzyme-linked immunosorbent assays to measure urinary free cortisone (E) and cortisol (F) and analyzed correlations between clinical measures reflecting mineralocorticoid action and 24-hour urinary excretion of E and F or their ratio, uE/F, which has been considered as the most sensitive index of renal 11beta-hydroxysteroid dehydrogenase type 2 activity. Two hundred nineteen healthy men were enrolled in this study. The uE/F ratio was 1.10 +/- 0.41 (mean +/- SD), and a strong linear correlation between uE and uF was observed in a double reciprocal plot. Urinary acid-labile aldosterone excretion had a negative correlation with 24-hour urinary Na excretion and Na/K ratio, but uE/F ratio had a weak positive correlation with the Na/K ratio and no significant correlation with 24-hour urinary Na excretion. In contrast, uE and uF had positive correlations with 24-hour urinary excretions of Na and K, raising the possibility of separate renal effects mediated by the glucocorticoid receptor. Furthermore, uE and uE/F ratio had strong negative correlations with urinary concentrations of Na and K. These results suggest that renal 11beta-hydroxysteroid dehydrogenase type 2 is an important regulatory factor of renal Na and K handlings independently of and/or complementary to the mineralocorticoid action of aldosterone.
authors.affiliation: Department of Diabetes and Endocrinology, Gifu University School of Medicine, Gifu 501-1194, Japan.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Isomura
authors.forename: Tomoatsu
authors.initials: T
authors.lastname: Mune
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morita
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Suwa
authors.forename: Nobuki
authors.initials: N
authors.lastname: Takada
authors.forename: Yoritsuna
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Jun
authors.initials: J
authors.lastname: Takeda
mesh_terms: D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000502:physiology*; D000328:Adult; D000450:Aldosterone / Q000097:blood; D003348:Cortisone / Q000652:urine; D003404:Creatinine / Q000097:blood; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006854:Hydrocortisone / Q000652:urine; D007668:Kidney / Q000201:enzymology* / Q000502:physiology*; D008297:Male; D008901:Mineralocorticoids / Q000502:physiology; D014882:Water-Electrolyte Balance / Q000502:physiology
chemical_list: D008901:Mineralocorticoids; D000450:Aldosterone; D003404:Creatinine; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2; D003348:Cortisone; D006854:Hydrocortisone