The effect of exercise and training status on platelet activation: do cocoa polyphenols play a role?
Sedentary and trained men respond differently to the same intensity of exercise, this is probably related to their platelet reactivity and antioxidant capacity. There is growing interest in the utilization of antioxidant-rich plant extracts as dietary food supplements. The aim of this study was to investigate the effect of an acute bout of sub maximal exercise on platelet count and differential response of platelet activation in trained and sedentary subjects and to observe if cocoa polyphenols reverse the effect of exercise on platelet function. The practical significance of this study was that many sedentary people engage in occasional strenuous exercise that may predispose them to risk of heart disease. Fasting blood samples were collected from 16 male subjects, pre and post 1-h cycling exercise at 70% of maximal aerobic power (VO2max) before and after consumption of cocoa or placebo. Agonist stimulated citrated whole blood was utilized for measuring platelet aggregation, adenosine triphosphate (ATP) release and platelet activation. Baseline platelet count (221 +/- 33 x 10(9)/L) and ATP release (1.4 +/- 0.6 nmol) increased significantly (P < 0.05) after exercise in all subjects. Baseline platelet numbers in the trained were higher (P < 0.05) than in the sedentary (235 +/- 37 vs. 208 +/- 34 x 10(9)/L), where as platelet activation in trained was lower (P < 0.05) than sedentary (51 +/- 6 vs. 59 +/- 5%). Seven days of cocoa polyphenol supplementation had little effect on any of the parameters measured. We conclude that trained subjects show decreased activation of stimulated platelets when compared to the sedentary subjects and short-term cocoa polyphenol supplementation did not decrease platelet activity in response to exercise independent of prior training status.
Singh
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I
Division of Laboratory Medicine, School of Medical Sciences, RMIT University Bundoora, Victoria 3083, Australia. indu.singh@rmit.edu.au
Quinn
H
H
Mok
M
M
Southgate
R J
RJ
Turner
A H
AH
Li
D
D
Sinclair
A J
AJ
Hawley
J A
JA
D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology; D002099:Cacao; D018592:Cross-Over Studies; D019587:Dietary Supplements; D004311:Double-Blind Method; D015444:Exercise / Q000502:physiology*; D005419:Flavonoids / Q000494:pharmacology; D044948:Flavonols / Q000494:pharmacology*; D006801:Humans; D008297:Male; D010636:Phenols / Q000494:pharmacology; D010809:Physical Fitness; D010936:Plant Extracts / Q000494:pharmacology; D015539:Platelet Activation / Q000187:drug effects* / Q000502:physiology*; D010976:Platelet Count; D059808:Polyphenols
D000975:Antioxidants; D005419:Flavonoids; D044948:Flavonols; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols