[Protective effects of granulocyte colony-stimulating factor on acute hepatic failure induced by D-galactosamine/lipopolysaccharide in mice].
OBJECTIVE
To evaluate the protective effect of recombinant human granulocyte colony stimulating factor (rhG-CSF) on acute hepatic failure induced by galactosamine (D-GalN) and lipopolysaccharide (LPS) in mice, and to explore its mechanism.


METHODS
The mice were intraperitoneally administered D-GalN (800 mg/kg) and LPS (10 microg/kg), and then were intraperitoneally injected either saline (the control group )or rhG-CSF at 300 microg/kg body weight (the therapy group) at 4 h, 2 h and 0 h before the D-GalN/LPS injection. The survival rate of the mice was estimated at 24 h after the D-GalN/LPS injection. The degree of hepatic injury was evaluated at 6 h after the D-GalN/LPS injection, and the levels of TNF-alpha, IFN-gamma, IL-6 and IL-10 mRNA were simultaneously measured by semiquantitative RT-PCR.


RESULTS
The survival rate of the therapy group was significantly higher than that of the control group (68.4% vs 20%, P<0.01). As compared with the control group, the degree of liver injury in the therapy group significantly decreased (P<0.05), and the levels of TNF-alpha and IFN-gamma mRNA in the hepatic tissue also reduced remarkably (P<0.01, respectively), while the levels of IL-6 and IL-10 mRNA increased (P<0.01, respectively) at 6 h after the D-GalN/LPS injection.


CONCLUSIONS
G-CSF can protect the mice from acute hepatic failure induced by D-GalN/LPS.
Xu
Xu-wen
XW
Institute of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China.
Tan
De-ming
DM
Lu
Meng-hou
MH
D000818:Animals; D005688:Galactosamine; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D008070:Lipopolysaccharides; D017114:Liver Failure, Acute / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D051379:Mice; D020011:Protective Agents / Q000627:therapeutic use; D011897:Random Allocation; D011994:Recombinant Proteins
D008070:Lipopolysaccharides; D020011:Protective Agents; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D005688:Galactosamine