An antigen produced by splicing of noncontiguous peptides in the reverse order.
CD8-positive T lymphocytes recognize peptides that are usually derived from the degradation of cellular proteins and are presented by class I molecules of the major histocompatibility complex. Here we describe a human minor histocompatibility antigen created by a polymorphism in the SP110 nuclear phosphoprotein gene. The antigenic peptide comprises two noncontiguous SP110 peptide segments spliced together in reverse order to that in which they occur in the predicted SP110 protein. The antigenic peptide could be produced in vitro by incubation of precursor peptides with highly purified 20S proteasomes. Cutting and splicing probably occur within the proteasome by transpeptidation.
Warren
Edus H
EH
Program in Immunology, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Vigneron
Nathalie J
NJ
Gavin
Marc A
MA
Coulie
Pierre G
PG
Stroobant
Vincent
V
Dalet
Alexandre
A
Tykodi
Scott S
SS
Xuereb
Suzanne M
SM
Mito
Jeffrey K
JK
Riddell
Stanley R
SR
Van den Eynde
Benoît J
BJ
D000483:Alleles; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D017951:Antigen Presentation; D001402:B-Lymphocytes / Q000276:immunology; D002461:Cell Line, Transformed; D003602:Cytotoxicity, Immunologic; D018274:Electroporation; D015234:HLA-A Antigens / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015778:Minor Histocompatibility Antigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D019154:Protein Splicing; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015234:HLA-A Antigens; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins; D010446:Peptide Fragments; C412425:Sp110 protein, human; D007371:Interferon-gamma; D046988:Proteasome Endopeptidase Complex