alpha-Tocopherol supplementation restores the reduction of erythrocyte glucose-6-phosphate dehydrogenase activity induced by forced training.
BACKGROUND
Erythrocyte glucose-6-phosphate dehydrogenase (G6PD) activity is closely related to free radical production. alpha-Tocopherol (alpha-T) is implicated with the reduction of lipid peroxidation.


OBJECTIVE
To investigate the effect of training and alpha-T supplementation on the erythrocyte G6PD activity.


METHODS
Blood was obtained from 10 basketball players pre-game (group A), post-game (group B) and after 30 days on alpha-T (dl-alpha-tocopheryl-acetate, 200mg 24h(-1) orally) supplementation pre- (group C) and post-training (group D). alpha-T and catecholamines were evaluated with HPLC methods and creatine kinase, lactate dehydrogenase, total antioxidant status (TAS) and G6PD activity with commercial kits.


RESULTS
TAS was increased in the groups with alpha-T addition (groups C and D). Post-exercise, TAS and G6PD activity were remarkably higher (2.10+/-0.13mmoll(-1), 7.92+/-1.5Ug(-1)Hb, respectively) in group D than those in group B (0.92+/-0.10mmoll(-1), 4.8+/-1.4Ug(-1)Hb, p<0.01, respectively). G6PD activity positively correlated with TAS (r=0.64, p<0.001) in all the studied groups.


CONCLUSIONS
Supplementation with alpha-T may protect G6PD activity from reduction induced by forced training.
Tsakiris
Stylianos
S
Department of Experimental Physiology, Medical School, University of Athens, P.O. Box 65257, GR-15401 Athens, Greece. stsakir@cc.uoa.gr
Reclos
George J
GJ
Parthimos
Theodore
T
Tsakiris
Theodore
T
Parthimos
Nickolaos
N
Schulpis
Kleopatra H
KH
D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D001490:Basketball; D002395:Catecholamines / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D004298:Dopamine / Q000097:blood; D004837:Epinephrine / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D015444:Exercise / Q000502:physiology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D009638:Norepinephrine / Q000097:blood; D014815:Vitamins / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000097:blood / Q000493:pharmacokinetics / Q000494:pharmacology*
D000975:Antioxidants; D002395:Catecholamines; D014815:Vitamins; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D003402:Creatine Kinase; D024502:alpha-Tocopherol; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine