Rhizosphere localized cationic peroxidase from barley roots is strongly activated by cadmium and correlated with root growth inhibition.
The activity of Cd-induced POD isozyme isolated from the surface of intact barley roots growing under some abiotic stress conditions (toxic metals: Al, Co, Cu, Hg; drought, NaCl, extreme temperatures: heat, cold) and compounds activating (2,4-D) or inhibiting (SHAM) POD activity as well as H(2)O(2) and H(2)O(2) scavenger (DTT) was characterized. Strong Cd concentration-dependent accumulation of one cationic POD isozyme was observed on PAGE, which correlated with Cd- and other stress induced root growth inhibition. This isoPOD is basic with isoelectric point about 9 and its localization is restricted only to the apical part of the barley root tip.
Tamás
Ladislav
L
Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 14, SK-84523 Bratislava, Slovak Republic. Ladislav.Tamas@savba.sk
Durceková
Katarína
K
Halusková
L'ubica
L
Huttová
Jana
J
Mistrík
Igor
I
Ollé
Marta
M
D002104:Cadmium / Q000633:toxicity*; D001467:Hordeum / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D009195:Peroxidase / Q000378:metabolism*; D027343:Rhizome / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D012987:Soil / Q000032:analysis*; D012989:Soil Pollutants / Q000633:toxicity*
D012987:Soil; D012989:Soil Pollutants; D002104:Cadmium; D009195:Peroxidase