Synthesis of D-labeled naphthyliodoacetamide and application to quantitative peptide analysis by isotope differential mass spectrometry.
D-labeled and -unlabeled N-beta-naphthyliodoacetamides have been synthesized for specific modification of the sulfhydryl groups of cysteine residues in proteins or peptides, and have been applied to quantitative analysis of several peptides. A combination of these reagents, coupled with mass spectrometry, is anticipated to serve as a useful tool for quantitative analysis of peptides and hence proteins.
Niwayama
Satomi
S
Department of Chemistry and Biochemistry, Texas Tech University, Box 41061, Lubbock, TX 79409-1061, USA. satomi.niwayama@ttu.edu <satomi.niwayama@ttu.edu>
Kurono
Sadamu
S
Cho
Hanjoung
H
Matsumoto
Hiroyuki
H
D007460:Iodoacetamide / Q000138:chemical synthesis* / Q000737:chemistry; D007554:Isotopes; D015394:Molecular Structure; D009281:Naphthalenes / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007554:Isotopes; D009281:Naphthalenes; D010455:Peptides; D007460:Iodoacetamide