title: Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system.
abstract: Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi-enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxide has been detected amperometrically. The linear concentration ranges are of pmol/L to nmol/L magnitude, with very low limits of detection. The proposed SIA system can be utilized reliably for the on-line simultaneous detection of creatine and creatinine in pharmaceutical products, as well as in serum samples, with a rate of 34 samples per hour and RSD values better than 0.16% (n=10).
authors.affiliation: Faculty of Chemistry, University of Bucharest, Bucharest, Romania. iustinavanstaden@yahoo.com
authors.forename: Raluca-Ioana
authors.initials: RI
authors.lastname: Stefan-van Staden
authors.forename: Rahel Girmai
authors.initials: RG
authors.lastname: Bokretsion
authors.forename: Jacobus F
authors.initials: JF
authors.lastname: van Staden
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry; D003401:Creatine / Q000032:analysis* / Q000097:blood; D003404:Creatinine / Q000032:analysis* / Q000097:blood; D004800:Enzymes, Immobilized / Q000737:chemistry; D017022:Flow Injection Analysis / Q000295:instrumentation*; D006861:Hydrogen Peroxide / Q000737:chemistry; D009862:Online Systems / Q000295:instrumentation*; D050879:Sarcosine Oxidase / Q000737:chemistry; D014512:Ureohydrolases / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D002244:Carbon; D003404:Creatinine; D006861:Hydrogen Peroxide; D050879:Sarcosine Oxidase; D000581:Amidohydrolases; C019946:creatininase; D014512:Ureohydrolases; C019944:creatinase; D003401:Creatine