Isolation and characterization of the P5 adhesin protein of Haemophilus parasuis serotype 5.
Haemophilus parasuis is a Gram-negative respiratory pathogen of young pigs that colonizes the upper respiratory tract and produces a number of symptoms collectiviely described as Glässer's disease. Recently, an H. parasuis P5-like outer membrane adhesin protein homologous to H. influenzae P5 was identified. The P5 adhesin was partially purified by anion exchange and size-exclusion chromatography. Final purification for functional studies was performed by elution of the protein from a polyacrylamide gel. Identification of the protein as a P5 adhesin homolog of H. influenzae was confirmed by N-terminal sequencing. The P5 protein had a molecular mass of 32,000 and a pI of 5.5. Unlike the H. influenzae P5 adhesin, the H. parasuis P5 protein did not bind carcinoembryonic antigen.
McVicker
Jerry K
JK
Iowa State University, Ames, Iowa, USA.
Tabatabai
Louisa B
LB
D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D002272:Carcinoembryonic Antigen / Q000737:chemistry; D044137:Haemophilus parasuis / Q000378:metabolism*; D015151:Immunoblotting; D025941:Protein Interaction Mapping; D020539:Sequence Analysis, Protein
D018829:Adhesins, Bacterial; D002272:Carcinoembryonic Antigen