title: Molecular cloning and functional expression of the Penaeus monodon 5-HT receptor.
abstract: Serotonin (5-HT) mediates a number of diverse physiological functions in crustaceans by interacting with various 5-HT receptor subtypes. A putative 5-HT receptor cloned from the ovary of the black tiger prawn (Penaeus monodon) consisted of 2291 nucleotides, encoding a putative 5-HT(1Pem) receptor protein of 591 amino acids. Transient expression of 5-HT(1Pem) in HEK293 cells demonstrated a saturable [3H]-5-HT binding with a Kd of 10.43+/-1.13 nM and Bmax of 1.53+/-0.06 pmol/mg. The putative 5-HT(1Pem) receptor is expressed in all tissues examined and is constitutively expressed in the ovary during ovarian maturation and spent phase. Polyclonal antibodies against the third intracellular loop (i3 loop) of the 5-HT receptor showed that the 5-HT(1Pem) receptor protein was expressed in the trabeculae of ovarian stages 1 and 2 but on the cortical rod and surrounding the oocyte membrane of stages 3 and 4, suggesting that receptor localization plays a critical role in regulating ovarian maturation and spawning in penaeus shrimp.
authors.affiliation: Institute of Molecular Biology and Genetics, Mahidol University (Salaya Campus), 25/25 Phutthamonthon 4 Rd. Salaya, Phutthamonthon district, Nakhon Pathom, 73170, Thailand. mbcov@mahidol.ac.th
authors.forename: Chalermporn
authors.initials: C
authors.lastname: Ongvarrasopone
authors.forename: Yaowaluck
authors.initials: Y
authors.lastname: Roshorm
authors.forename: Suthasinee
authors.initials: S
authors.lastname: Somyong
authors.forename: Chetsada
authors.initials: C
authors.lastname: Pothiratana
authors.forename: Soontaree
authors.initials: S
authors.lastname: Petchdee
authors.forename: Jarasporn
authors.initials: J
authors.lastname: Tangkhabuanbutra
authors.forename: Samaisukh
authors.initials: S
authors.lastname: Sophasan
authors.forename: Sakol
authors.initials: S
authors.lastname: Panyim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010053:Ovary / Q000737:chemistry / Q000166:cytology / Q000254:growth & development; D033561:Penaeidae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D044263:Receptors, Serotonin, 5-HT1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D044263:Receptors, Serotonin, 5-HT1; D012701:Serotonin