Structure of the O-antigen of Providencia stuartii O20, a new polysaccharide containing 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-d-galacto-non-2-ulosonic acid.
The O-polysaccharide chain of the lipopolysaccharide (LPS) of Providencia stuartii O20 was found to contain d-glucuronic acid, N-acetyl-d-glucosamine, and a rarely occurring higher sugar 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-d-galacto-non-2-ulosonic acid (di-N-acetyl-8-epilegionaminic acid, 8eLeg5Ac7Ac). Degradation of the LPS with dilute acetic acid caused depolymerization of the polysaccharide chain by the ketosidic linkage to give a tetrasaccharide corresponding to the repeating unit of the polysaccharide. Based on sugar and methylation analyses of the tetrasaccharide and O-deacylated LPS as well as ESIMS, (1)H and (13)C NMR spectroscopy data, the structure of the O-polysaccharide of P. stuartii O20 was established.
Shashkov
Alexander S
AS
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Kocharova
Nina A
NA
Zatonsky
George V
GV
Błaszczyk
Aleksandra
A
Knirel
Yuriy A
YA
Rozalski
Antoni
A
D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D011532:Providencia / Q000737:chemistry*; D012794:Sialic Acids / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization
D019081:O Antigens; D012794:Sialic Acids; C050215:5,7-diacetamido-3,5,7,9-tetradeoxy-glycerogalacto-nonulosonic acid