A modified filter paper culture technique for screening of Strongyloides stercoralis ivermectin sensitivity in clinical specimens.
An in vitro test for the detection of ivermectin sensitivity of Strongyloides stercoralis in human clinical specimens was developed. The test has a simple procedure that combines parasite culture with a drug assay. It is based on the principle of the drug effect on larval development test, combined with a modified coproculture using the filter paper culture technique. This does not require parasite isolation, and drug sensitivity can be shown in relationship to the dose. This test can be applied in field surveys for anthelmintic sensitivity and for appropriate drug rotation strategies when drug resistance occurs.
Intapan
Pewpan M
PM
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. pewpan@kku.ac.th
Prasongdee
Thidarat K
TK
Laummaunwai
Porntip
P
Sawanyawisuth
Kittisak
K
Singthong
Saree
S
Maleewong
Wanchai
W
D000818:Animals; D000977:Antiparasitic Agents / Q000494:pharmacology*; D007559:Ivermectin / Q000494:pharmacology*; D010209:Paper; D017171:Strongyloides stercoralis / Q000187:drug effects*
D000977:Antiparasitic Agents; D007559:Ivermectin