title: Dendritic cell surface calreticulin is a receptor for NY-ESO-1: direct interactions between tumor-associated antigen and the innate immune system.
abstract: How the immune system recognizes endogenously arising tumors and elicits adaptive immune responses against nonmutated tumor-associated Ags is poorly understood. In search of intrinsic factors contributing to the immunogenicity of the tumor-associated Ag NY-ESO-1, we found that the NY-ESO-1 protein binds to the surface of immature dendritic cells (DC), macrophages, and monocytes, but not to that of B cells or T cells. Using immunoprecipitation coupled with tandem mass spectrometry, we isolated DC surface calreticulin as the receptor for NY-ESO-1. Calreticulin Abs blocked NY-ESO-1 binding on immature DC and its cross-presentation to CD8+ T cells in vitro. Calreticulin/NY-ESO-1 interactions provide a direct link between NY-ESO-1, the innate immune system, and, potentially, the adaptive immune response against NY-ESO-1.
authors.affiliation: Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA. gzeng@mednet.ucla.edu
authors.forename: Gang
authors.initials: G
authors.lastname: Zeng
authors.forename: Michael E
authors.initials: ME
authors.lastname: Aldridge
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Tian
authors.forename: Daniel
authors.initials: D
authors.lastname: Seiler
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Zhang
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Jin
authors.forename: Jianyu
authors.initials: J
authors.lastname: Rao
authors.forename: Weidong
authors.initials: W
authors.lastname: Li
authors.forename: Dequan
authors.initials: D
authors.lastname: Chen
authors.forename: Marlyn P
authors.initials: MP
authors.lastname: Langford
authors.forename: Chris
authors.initials: C
authors.lastname: Duggan
authors.forename: Arie S
authors.initials: AS
authors.lastname: Belldegrun
authors.forename: Steven M
authors.initials: SM
authors.lastname: Dubinett
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D037282:Calreticulin / Q000378:metabolism*; D002454:Cell Differentiation / Q000276:immunology; D002460:Cell Line; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007113:Immunity, Innate; D008264:Macrophages / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D011485:Protein Binding / Q000276:immunology; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; D037282:Calreticulin; D008565:Membrane Proteins; D011956:Receptors, Cell Surface