title: The self-regulating nature of spontaneous synchronized activity in developing mouse cortical neurones.
abstract: Waves of spontaneous electrical activity that are highly synchronized across large populations of neurones occur throughout the developing mammalian central nervous system. The stages at which this activity occurs are tightly regulated to allow activity-dependent developmental programmes to be initiated correctly. What determines the onset and cessation of spontaneous synchronous activity (SSA) in a particular region of the nervous system, however, remains unclear. We have tested the hypothesis that activity itself triggers developmental changes in intrinsic and circuit properties that determine the stages at which SSA occurs. To do this we exposed cultured slices of mouse neocortex to tetrodotoxin (TTX) to block SSA, which normally occurs between embryonic day 17 (E17) and postnatal day 3 (P3). In control cultured slices, SSA rarely occurs after P3. In TTX-treated slices, however, SSA was generated from P3 (the day of TTX removal) until at least P10. This indicates that in the absence of spontaneous activity, the mechanisms that normally determine the timing of SSA are not initiated, and that a compensatory response occurs that shifts the time of SSA occurrence to later developmental stages.
authors.affiliation: Department of Biology, University of Washington, Seattle, WA 98195, USA.
authors.forename: Annette K
authors.initials: AK
authors.lastname: McCabe
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Chisholm
authors.forename: Heidi L
authors.initials: HL
authors.lastname: Picken-Bahrey
authors.forename: William J
authors.initials: WJ
authors.lastname: Moody
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D001683:Biological Clocks / Q000502:physiology*; D002540:Cerebral Cortex / Q000196:embryology* / Q000502:physiology*; D005246:Feedback / Q000502:physiology; D051379:Mice; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000502:physiology*