title: Phosphonic acid monolayers for binding of bioactive molecules to titanium surfaces.
abstract: Two different phosphonic acid monolayer films for immobilization of bioactive molecules such as the protein BMP-2 on titanium surfaces have been prepared. Monolayers of (11-hydroxyundecyl)phosphonic acid and (12-carboxydodecyl)phosphonic acid molecules were produced by a simple dipping process (the T-BAG method). The terminal functional groups on these monolayers were activated (carbonyldiimidazole for hydroxyl groups and N-hydroxysuccinimide for carboxyl groups) to bind amine-containing molecules. The reactivity of the surfaces was investigated using trifluoroethylamine hydrochloride and BMP-2. Each step of the surface modification procedure was characterized by X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry.
authors.affiliation: Institut für Technische Chemie, Abt. TC Makromolekularer Stoffe, Technische Universität Braunschweig, Hans-Sommer-Strasse 10, 38106 Braunschweig, Germany.
authors.forename: Nina
authors.initials: N
authors.lastname: Adden
authors.forename: Lara J
authors.initials: LJ
authors.lastname: Gamble
authors.forename: David G
authors.initials: DG
authors.lastname: Castner
authors.forename: Andrea
authors.initials: A
authors.lastname: Hoffmann
authors.forename: Gerhard
authors.initials: G
authors.lastname: Gross
authors.forename: Henning
authors.initials: H
authors.lastname: Menzel
mesh_terms: D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D005021:Ethylamines / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D063065:Organophosphonates / Q000737:chemistry*; D011485:Protein Binding; D013057:Spectrum Analysis; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000737:chemistry*; D014965:X-Rays
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D020099:Coated Materials, Biocompatible; D005021:Ethylamines; D063065:Organophosphonates; D016212:Transforming Growth Factor beta; C032626:trifluoroethylamine; D014025:Titanium