Comparison of API 20E and invA PCR for identification of Salmonella enterica isolates from swine production units.
API 20E and invA PCR were evaluated for the identification of Salmonella enterica isolates from swine farms. API 20E had the highest agreement with other tests at the 99.9% likelihood level. Both tests had 100% sensitivity and 96% specificity compared to 16S rRNA sequencing. Compared to serotyping, both tests had 96% sensitivity; specificity was 86% for API 20E and 79% for invA PCR.
Nucera
Daniele M
DM
Department of Pathobiology, University of Illinois, 2001 South Lincoln Avenue, Urbana, 61802, USA.
Maddox
Carol W
CW
Hoien-Dalen
Patricia
P
Weigel
Ronald M
RM
D000822:Animal Husbandry; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods; D001431:Bacteriological Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013552:Swine
D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; C075518:invA protein, Bacteria