title: Polyhydroxyalkanoate (PHA) biosynthesis from structurally unrelated carbon sources by a newly characterized Bacillus spp.
abstract: A newly acquired polyhydroxyalkanoate (PHA) producing Bacillus spp. was identified to be a strain of Bacillus cereus using a range of microbiological and molecular techniques. This strain, named B. cereus SPV, was found to be capable of using a wide range of carbon sources including glucose, fructose, sucrose, various fatty acids and gluconate for the production of PHAs, an advantage for the commercial production of the polymers. The media used for the polymer production was novel in the context of the genus Bacillus. The PHA, once produced, was found to remain at a constant maximal concentration, without any degradation, a great advantage for the commercial production of the PHAs. This particular strain of Bacillus spp. was able to synthesize various PHAs with 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB)-like monomer units from structurally unrelated carbon sources such as fructose, sucrose and gluconate. This is the first report of the incorporation of a 4HB related monomer containing PHA by the genus Bacillus and from structurally unrelated carbon sources. The PHAs isolated had molecular weights ranging between (0.4 and 0.8) x 10(6) and low polydispersity index values (M(W)/M(N)) ranging from 2.6 to 3.4.
authors.affiliation: Department of Molecular and Applied Biosciences, School of Biosciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK.
authors.forename: S P
authors.initials: SP
authors.lastname: Valappil
authors.forename: D
authors.initials: D
authors.lastname: Peiris
authors.forename: G J
authors.initials: GJ
authors.lastname: Langley
authors.forename: J M
authors.initials: JM
authors.lastname: Herniman
authors.forename: A R
authors.initials: AR
authors.lastname: Boccaccini
authors.forename: C
authors.initials: C
authors.lastname: Bucke
authors.forename: I
authors.initials: I
authors.lastname: Roy
mesh_terms: D001409:Bacillus cereus / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D006880:Hydroxy Acids / Q000378:metabolism*; D007218:Industrial Microbiology; D008969:Molecular Sequence Data; D011091:Polyesters / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D006880:Hydroxy Acids; D011091:Polyesters; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S