Involvement of DRG11 in the development of the primary afferent nociceptive system.
During development, dorsal root ganglia (DRG) neurons differentiate in various subpopulations, nociceptive neurons belonging in the small-diameter class. This study addresses the role played by DRG11, a transcription factor expressed in the spinal area of projection of small-diameter DRG neurons, in the development of the primary afferent system. The various subclasses of DRG neurons were compared between wild-type and Drg11(-/-) mice at embryonic and postnatal life. In Drg11(-/-) mice, numbers of small peptidergic and non-peptidergic DRG neurons were decreased at P7 concomitant with abnormal cell death. Innervation by small DRG neurons was impaired in cutaneous, visceral and deep tissues. Large DRG neurons were not affected. The data point to a role for DRG11 in early postnatal survival of normally generated small primary afferent neurons innervating various kinds of peripheral tissues, which would explain the nociceptive deficits observed in Drg11-null mutant mice.
Rebelo
Sandra
S
Laboratory of Molecular Cell Biology, University of Oporto, Porto, Portugal.
Chen
Zhou-Feng
ZF
Anderson
David J
DJ
Lima
Deolinda
D
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000502:physiology; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002452:Cell Count; D004622:Embryo, Mammalian; D005727:Ganglia, Spinal / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D018398:Homeodomain Proteins / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D007719:Knee Joint / Q000294:innervation; D037102:Lectins / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000502:physiology*; D016900:Neurofilament Proteins / Q000378:metabolism; D009475:Neurons, Afferent / Q000145:classification / Q000502:physiology*; D009619:Nociceptors / Q000502:physiology*; D012867:Skin / Q000294:innervation; D014157:Transcription Factors / Q000172:deficiency / Q000502:physiology*; D001743:Urinary Bladder / Q000294:innervation
D018398:Homeodomain Proteins; D037102:Lectins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C433766:Prrxl1 protein, mouse; D014157:Transcription Factors; C051774:neurofilament protein H; D053148:Caspase 3; D015740:Calcitonin Gene-Related Peptide