Using triplet-triplet energy transfer to measure conformational dynamics in polypeptide chains.
Intrachain diffusion processes play an important role in protein folding and function. In this chapter we discuss the application of triplet-triplet energy transfer to directly measure rate constants for intrachain contact formation in polypeptide chains. The photochemistry of triplet-triplet energy transfer is described, experimental prerequisites of the method are discussed, and a detailed description of the experimental protocols and data analysis is given.
Fierz
Beat
B
Department of Biophysical Chemistry, Biozentrum, University of Basel, Basel, Switzerland.
Joder
Karin
K
Krieger
Florian
F
Kiefhaber
Thomas
T
D000596:Amino Acids / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D010777:Photochemistry / Q000379:methods*; D011487:Protein Conformation; D017510:Protein Folding; D044004:Xanthones / Q000737:chemistry
D000596:Amino Acids; D010455:Peptides; D044004:Xanthones; C009689:xanthone