title: The solution structure of the invasive tip complex from Afa/Dr fibrils.
abstract: Afa/Dr family of adhesins are produced by pathogenic Escherichia coli strains that are especially prevalent in chronic diarrhoeal and recurrent urinary tract infections. Most notably, they are found in up to 50% of cystitis cases in children and 30% of pyelonephritis in pregnant women. Afa/Dr adhesins are capped surface fibrils that mediate recognition of the host and subsequent bacterial internalization. Using the newly solved three-dimensional structure of the minimal invasive complex (AfaDE) combined with biochemical and cellular assays, we reveal the architecture of the fibrillar cap and identify a novel mode of synergistic integrin recognition.
authors.affiliation: Division of Molecular Biosciences, Biochemistry Building, Imperial College London, South Kensington, London SW7 2AZ, UK.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Cota
authors.forename: Celine
authors.initials: C
authors.lastname: Jones
authors.forename: Peter
authors.initials: P
authors.lastname: Simpson
authors.forename: Harri
authors.initials: H
authors.lastname: Altroff
authors.forename: Kirstine L
authors.initials: KL
authors.lastname: Anderson
authors.forename: Laurence
authors.initials: L
authors.lastname: du Merle
authors.forename: Julie
authors.initials: J
authors.lastname: Guignot
authors.forename: Alain
authors.initials: A
authors.lastname: Servin
authors.forename: Chantal
authors.initials: C
authors.lastname: Le Bouguénec
authors.forename: Helen
authors.initials: H
authors.lastname: Mardon
authors.forename: Stephen
authors.initials: S
authors.lastname: Matthews
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion / Q000235:genetics / Q000502:physiology; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding
chemical_list: D018830:Adhesins, Escherichia coli; C465564:Dr adhesin, E coli; D029968:Escherichia coli Proteins