Viable Mycoplasma mycoides ssp. mycoides small colony-mediated depression of the bovine cell responsiveness to the mitogen concanavalin A.
Mycoplasma mycoides ssp. mycoides biotype Small Colony (MmmSC) is the causative agent of contagious bovine pleuropneumonia (CBPP), which is still a major tropical cattle disease. Development of an efficient vaccine requires an understanding of the immunopathology of CBPP as MmmSC presents a strong ability to escape the host immune response. The objective of this study was to determine whether the presence of MmmSC can modulate the immune response induced by the mitogen Concanavalin A (ConA) on bovine immune cells [peripheral blood mononuclear cells (PBMC) and lymph node (LN) cells]. Comparative analysis of the immunomodulating properties of viable versus heat-killed MmmSC on ConA-stimulated immune cells revealed that while heat-killed MmmSC had no effect, viable MmmSC strongly depressed, in a concentration-dependent manner, the ConA mitogenic activity (blastogenesis and interferon-gamma production). Both B-cell and T-cell activation were affected with the highest impact on the CD4 T cells. The phenotypic analysis showed that the ConA-induced proliferation of CD25(+) cells was strongly reduced when co-exposed to viable MmmSC, confirming that events associated with ConA-induced cell activation were suppressed by the pathogen. This study thus demonstrated that viable MmmSC is able to inhibit the polyclonal mitogenic activity of the ConA on bovine PBMC and LN cells. This finding strongly suggests that the persistence of viable MmmSC may also thus inhibit the bovine immune response directed towards inactivated MmmSC, whether dead or in the form of antigens, also present during infection. This study confirmed that MmmSC has evolved an efficient mechanism to prevent its elimination from the host.
Dedieu
L
L
Centre de Cooperation Internationale en Recherche Agronomique pour le Developpement (CIRAD), Montpellier, France. laurence.dedieu@cirad.fr
Balcer-Rodrigues
V
V
D000818:Animals; D002417:Cattle; D002469:Cell Separation; D015169:Colony Count, Microbial; D003208:Concanavalin A / Q000494:pharmacology*; D006358:Hot Temperature; D007165:Immunosuppression Therapy; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000382:microbiology*; D008213:Lymphocyte Activation / Q000276:immunology; D008934:Mitogens / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009176:Mycoplasma mycoides / Q000254:growth & development* / Q000472:pathogenicity
D008934:Mitogens; D003208:Concanavalin A; D007371:Interferon-gamma