Isolation and characterization of a new T-even bacteriophage, CEV1, and determination of its potential to reduce Escherichia coli O157:H7 levels in sheep.
Bacteriophage CEV1 was isolated from sheep resistant to Escherichia coli O157:H7 colonization. In vitro, CEV1 efficiently infected E. coli O157:H7 grown both aerobically and anaerobically. In vivo, sheep receiving a single oral dose of CEV1 showed a 2-log-unit reduction in intestinal E. coli O157:H7 levels within 2 days compared to levels in the controls.
Raya
Raul R
RR
The Evergreen State College, Olympia, WA 98505, USA.
Varey
Peter
P
Oot
Rebecca A
RA
Dyen
Michael R
MR
Callaway
Todd R
TR
Edrington
Tom S
TS
Kutter
Elizabeth M
EM
Brabban
Andrew D
AD
D000284:Administration, Oral; D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D019453:Escherichia coli O157 / Q000302:isolation & purification / Q000472:pathogenicity / Q000821:virology*; D005260:Female; D005516:Food Microbiology; D008854:Microscopy, Electron; D019936:Probiotics; D012756:Sheep / Q000382:microbiology*; D013604:T-Phages / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure