title: Genetic analysis of Thailand hantavirus in Bandicota indica trapped in Thailand.
abstract: Sixty one tissue samples from several rodent species trapped in five provinces of Thailand were examined for the presence of hantaviral markers by enzyme-immunoassay and immunoblotting. Four samples, all from the great bandicoot rat Bandicota indica, were confirmed positive for the hantaviral N-antigen. Two of them were trapped in Nakhon Pathom province, the other two in Nakhon Ratchasima province, approximately 250 km from the other trapping site. When analysed by RT-nested PCR, all four rodents were found positive for the hantaviral S- and M-segment nucleotide sequences. Genetic analysis revealed that the four newly described wild-type strains belong to Thailand hantavirus. On the phylogenetic trees they formed a well-supported cluster within the group of Murinae-associated hantaviruses and shared a recent common ancestor with Seoul virus.
authors.affiliation: OSEB, UMR 5202 du CNRS, Muséum National d'Histoire naturelle, Paris, France. hugot@mnhn.fr
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Hugot
authors.forename: Angelina
authors.initials: A
authors.lastname: Plyusnina
authors.forename: Vincent
authors.initials: V
authors.lastname: Herbreteau
authors.forename: Kirill
authors.initials: K
authors.lastname: Nemirov
authors.forename: Juha
authors.initials: J
authors.lastname: Laakkonen
authors.forename: Ake
authors.initials: A
authors.lastname: Lundkvist
authors.forename: Yupin
authors.initials: Y
authors.lastname: Supputamongkol
authors.forename: Heikki
authors.initials: H
authors.lastname: Henttonen
authors.forename: Alexander
authors.initials: A
authors.lastname: Plyusnin
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D016679:Genome, Viral; D006476:Orthohantavirus / Q000235:genetics* / Q000302:isolation & purification; D015151:Immunoblotting / Q000379:methods; D008969:Molecular Sequence Data; D051189:Murinae / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013785:Thailand
chemical_list: D000956:Antigens, Viral