title: Vascular endothelial growth factor and matrix metalloproteinase-2 expedite formation of endometriosis in the early stage ICR mouse model.
abstract: OBJECTIVE
  To establish a mouse model for endometriosis and to evaluate roles of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) in the formation of disease.
  
  
  METHODS
  Experimental laboratory study.
  
  
  METHODS
  A women's hospital in China.
  
  
  METHODS
  Ten women with endometriosis and 10 control women, as well as ICR mice.
  
  
  METHODS
  Endometrial fragments were transplanted in the peritoneal cavities of mice at minilaparotomy. Transplants were observed and then removed for the assessment of morphology and immunohistochemical staining of VEGF and MMP-2.
  
  
  METHODS
  Observation of transplants, expression of VEGF and MMP-2.
  
  
  RESULTS
  On days 1 and 2, glandular and stromal cells were viable at the margins of transplants. On day 3, the transplants were surrounded by mesothelial cells, and the endometrial glands and stromal cells were clearly viable at the interface. The scores of VEGF and MMP-2 of viable glandular cells of transplants were increased compared with the ones before transplantation. The scores of VEGF and MMP-2 of transplants from women with endometriosis were higher than those of control women.
  
  
  CONCLUSIONS
  Endometrial transplants from the patients with endometriosis express more VEGF and MMP-2 than endometrium in control women, suggesting that VEGF and MMP-2 may expedite the formation of endometriosis in its early stage.
authors.affiliation: Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors.forename: Xiu-E
authors.initials: XE
authors.lastname: Lu
authors.forename: Wei-Xuan
authors.initials: WX
authors.lastname: Ning
authors.forename: Min-Yue
authors.initials: MY
authors.lastname: Dong
authors.forename: Ai-Xia
authors.initials: AX
authors.lastname: Liu
authors.forename: Fan
authors.initials: F
authors.lastname: Jin
authors.forename: He-Feng
authors.initials: HF
authors.lastname: Huang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004715:Endometriosis / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D015260:Neprilysin / Q000378:metabolism; D016378:Tissue Transplantation; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D007633:Keratins; D015260:Neprilysin; D020778:Matrix Metalloproteinase 2