Effects of post-treatment with direct hemoperfusion using a CTR column on mortality and inflammatory responses to endotoxin-induced shock in rats.
BACKGROUND
To clarify the effects of post-treatment with direct hemoperfusion using a CTR column on the mortality and inflammatory responses to endotoxin-induced shock in rats.


METHODS
Thirty-six male rats were randomly assigned to 1 of 3 groups (n = 12/group): the endotoxemic group, receiving intravenous Escherichia coli endotoxin (15 mg/kg over 2 min); the control column group, treated without CTR for 120 min at 2 h after endotoxin injection, and CTR-post-treatment group, treated with CTR for 120 min at 2 h after endotoxin injection. Hemodynamics, arterial blood gases, and mortality were recorded for the 8-hour observation period, and plasma cytokine concentrations were measured every 4 h.


RESULTS
The mortality rates were 83, 83 and 33% for the endotoxemic, control column, and CTR post-treatment groups, respectively. The increases in IL-6 concentrations were less for the CTR post-treatment group than the other 2 groups.


CONCLUSIONS
The present study shows that CTR post-treatment inhibited hypotension and elevations in IL-6 concentrations, reducing the mortality rate of rats with endotoxin-induced shock.
Taniguchi
Takumi
T
Department of Emergency and Critical Care Medicine, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan. takutaniyan@yahoo.co.jp
Takemoto
Yasuhiro
Y
Tsuda
Kazunobu
K
Inaba
Hideo
H
Yamamoto
Ken
K
D000818:Animals; D001784:Blood Gas Analysis; D004731:Endotoxins / Q000633:toxicity*; D006464:Hemoperfusion / Q000295:instrumentation; D007022:Hypotension / Q000097:blood / Q000139:chemically induced; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012769:Shock / Q000097:blood* / Q000139:chemically induced; D013997:Time Factors
D004731:Endotoxins; D015850:Interleukin-6; C044101:endotoxin, Escherichia coli