Horse embryonic stem cell lines from the proliferation of inner cell mass cells.
Inner cell mass (ICM) cells were isolated immunosurgically from day 7-8 horse blastocysts and, after proliferation in vitro for 15-28 passages, three lines of cells were confirmed to be embryonic stem (ES) cells by their continued expression of alkaline phosphatase activity and their ability to bind antisera specific for the recognized stem cell markers, SSEA-1, TRA-1-60, TRA-1-81, and the key embryonic gene Oct-4. When maintained under feeder cell-free conditions in vitro, the three lines of cells differentiated into cells of ectodermal, endodermal, and mesodermal lineages. However, they did not form teratomata when injected into the testes of severe combined immunodeficiency (SCID)/beige immunoincompetent mice, thereby indicating a significant difference in phenotype between ES cells of the horse and those of the mouse and human.
Li
Xihe
X
Department of Veterinary Medicine, Equine Fertility Unit, Mertoun Paddocks, University of Cambridge, Newmarket, Suffolk CB8 9BH, UK.
Zhou
S G
SG
Imreh
Marta P
MP
Ahrlund-Richter
L
L
Allen
W R
WR
D000818:Animals; D015415:Biomarkers; D001755:Blastocyst / Q000166:cytology*; D049109:Cell Proliferation; D004622:Embryo, Mammalian / Q000166:cytology*; D018397:Equidae / Q000378:metabolism*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D013724:Teratoma / Q000473:pathology
D015415:Biomarkers