title: Effect of F-spondin on cementoblastic differentiation of human periodontal ligament cells.
abstract: Cementum is a mineralized tissue produced by cementoblasts covering the roots of teeth that provides for the attachment of periodontal ligament to roots and surrounding alveolar bone. To study the mechanism of proliferation and differentiation of cementoblasts is important for understanding periodontal physiology and pathology including periodontal tissue regeneration. However, the detailed mechanism of the proliferation and differentiation of human cementoblasts is still unclear. We previously established human cementoblast-like (HCEM) cell lines. We thought that comparing the transcriptional profiles of HCEM cells and human periodontal ligament (HPL) cells derived from the same teeth could be a good approach to identify genes that influence the nature of cementoblasts. We identified F-spondin as the gene demonstrating the high fold change expression in HCEM cells. Interestingly, F-spondin highly expressing HPL cells showed similar phenotype of cementoblasts, such as up-regulation of mineralized-related genes. Overall, we identified F-spondin as a promoting factor for cementoblastic differentiation.
authors.affiliation: Department of Oral and Maxillofacial Pathobiology, Graduate School of Biomedical Sciences, Hiroshima University, Japan.
authors.forename: Masae
authors.initials: M
authors.lastname: Kitagawa
authors.forename: Yasusei
authors.initials: Y
authors.lastname: Kudo
authors.forename: Shinji
authors.initials: S
authors.lastname: Iizuka
authors.forename: Ikuko
authors.initials: I
authors.lastname: Ogawa
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Abiko
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Miyauchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Takata
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D003739:Dental Cementum / Q000166:cytology* / Q000378:metabolism*; D016326:Extracellular Matrix Proteins; D005786:Gene Expression Regulation; D006801:Humans; D009928:Organ Specificity; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism*
chemical_list: D016326:Extracellular Matrix Proteins; D010455:Peptides; C429104:SPON1 protein, human