Involvement of IFNbeta on IFNgamma and nitric oxide (NO) production by bone marrow (BM) cells in response to lipopolysaccharide.
Bone marrow (BM) cells fractioned in Percoll gradients yield a low-density fraction (Fr3) highly enriched in suppressor activity. Previously, it has been demonstrated that BM associated suppressor activity was mediated by early myeloid cells, through a mechanism dependent on endogenous IFNgamma and nitric oxide production after bacterial stimuli, e.g. lipopolysaccharide (LPS). However, the mechanism(s) through which the IFNgamma is produced in BM has not yet been fully elucidated. Therefore, in the present study we investigated the involvement of IL-12, IL-18 and IFNbeta on the production of IFNgamma and nitric oxide in cultures of BM Fr3 cells, and characterized the IFNgamma-producing cells, in response to LPS. The results show that both IL-12 and IFNbeta, but not IL-18, are involved on IFNgamma production. However, only IFNbeta appears to be critical on nitric oxide production. Furthermore, we found that cells of the Thy1.2+CD3+ phenotype produce IFNgamma and are tightly involved on nitric oxide production by BM Fr3 cells. In conclusion, IFNbeta appears to be critical on IFNgamma- and nitric oxide production by BM cells in response to LPS, through a mechanism that is dependent on Thy1.2+CD3+ IFNgamma-producing cells.
Campillo
J A
JA
Immunology Service, Hospital Clínico San Carlos, Madrid, Spain; Inserm U659, Faculté de Médicine de Créteil, Université Paris-XII, 8, rue du Général-Sarrail, 94010 Créteil cedex, France. jcampillo68@hotmail.com
Peláez
B
B
Angulo
I
I
Bensussan
A
A
Subiza
J L
JL
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D019070:Cell Lineage; D005260:Female; D016899:Interferon-beta / Q000502:physiology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000502:physiology; D020382:Interleukin-18 / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000378:metabolism
D020382:Interleukin-18; D008070:Lipopolysaccharides; D018664:Interleukin-12; D009569:Nitric Oxide; D016899:Interferon-beta; D007371:Interferon-gamma