title: Therapeutic and preventive properties of quercetin in experimental arthritis correlate with decreased macrophage inflammatory mediators.
abstract: Pentahydroxyflavone dihydrate, quercetin (QU) is one of common flavonols biosynthesized by plants and has been suggested to modulate inflammatory responses in various models. In the present study, we investigated in vivo effects of oral or intra-cutaneous QU in chronic rat adjuvant-induced arthritis (AA). Growth delay and arthritic scores were evaluated daily after AA induction in Lewis rats. Oral administration of QU (5 x 160 mg/kg) to arthritic rats resulted in a clear decrease of clinical signs compared to untreated controls. Intra-cutaneous injections of lower doses (5 x 60 mg/kg) of QU gave similar anti-arthritic effects, while 5 x 30 mg/kg concentrations were inefficient in this respect. Finally, injection of relatively low QU doses (5 x 30 mg/kg) prior to AA induction significantly reduced arthritis signs. As QU was suggested to inhibit macrophage-derived cytokines and nitric oxide (NO), we then analyzed macrophage response ex vivo. Anti-arthritic effects of QU correlated with significant decrease of inflammatory mediators produced by peritoneal macrophages, ex vivo and in vitro. These data indicate that QU is a potential anti-inflammatory therapeutic and preventive agent targeting the inflammatory response of macrophages.
authors.affiliation: Laboratory of Immunology and Parasitology, EA3677, Faculty of Pharmacy, Bordeaux 2 University, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Mamani-Matsuda
authors.forename: Tina
authors.initials: T
authors.lastname: Kauss
authors.forename: Abir
authors.initials: A
authors.lastname: Al-Kharrat
authors.forename: Jérôme
authors.initials: J
authors.lastname: Rambert
authors.forename: Fawaz
authors.initials: F
authors.lastname: Fawaz
authors.forename: Denis
authors.initials: D
authors.lastname: Thiolat
authors.forename: Daniel
authors.initials: D
authors.lastname: Moynet
authors.forename: Sara
authors.initials: S
authors.lastname: Coves
authors.forename: Denis
authors.initials: D
authors.lastname: Malvy
authors.forename: M Djavad
authors.initials: MD
authors.lastname: Mossalayi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control; D005260:Female; D018836:Inflammation Mediators / Q000276:immunology*; D007271:Injections, Intradermal; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D011794:Quercetin / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D018836:Inflammation Mediators; D011794:Quercetin; D052247:Nitric Oxide Synthase Type II