title: DNMT1 and HDAC1 gene expression in impaired spermatogenesis and testicular cancer.
abstract: DNA methylation catalyzed by DNA methyltransferases (DNMTs) and histone deacetylation catalyzed by histone deacetylases (HDACs) play an important role for the regulation of gene expression during carcinogenesis and spermatogenesis. We therefore studied the cell-specific expression of DNMT1 and HDAC1 for the first time in human testicular cancer and impaired human spermatogenesis. During normal spermatogenesis, DNMT1 and HDAC1 were colocalized in nuclei of spermatogonia. While HDAC1 was additionally present in nuclei of Sertoli cells, DNMT1 was restricted to germ cells exhibiting a different expression pattern of mRNA (in pachytene spermatocytes and round spermatids) and protein (in round spermatids). Interestingly, in infertile patients revealing round spermatid maturation arrest, round spermatids lack DNMT1 protein, while pachytene spermatocytes became immunopositive for DNMT1. In contrast, no changes in the expression pattern could be observed for HDAC1. This holds true also in testicular tumors, where HDAC1 has been demonstrated in embryonal carcinoma, seminoma and teratoma. Interestingly, DNMT1 was not expressed in seminoma, but upregulated in embryonal carcinoma.
authors.affiliation: Department of Urology and Pediatric Urology, Giessen, Germany.
authors.forename: Olufunmilade A
authors.initials: OA
authors.lastname: Omisanjo
authors.forename: Katharina
authors.initials: K
authors.lastname: Biermann
authors.forename: Sonja
authors.initials: S
authors.lastname: Hartmann
authors.forename: Lukas C
authors.initials: LC
authors.lastname: Heukamp
authors.forename: Violetta
authors.initials: V
authors.lastname: Sonnack
authors.forename: Anne
authors.initials: A
authors.lastname: Hild
authors.forename: Ralph
authors.initials: R
authors.lastname: Brehm
authors.forename: Martin
authors.initials: M
authors.lastname: Bergmann
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Weidner
authors.forename: Klaus
authors.initials: K
authors.lastname: Steger
mesh_terms: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019175:DNA Methylation; D005786:Gene Expression Regulation; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008297:Male; D009373:Neoplasms, Germ Cell and Embryonal / Q000235:genetics / Q000378:metabolism*; D013087:Spermatids / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D013091:Spermatogenesis; D013093:Spermatogonia / Q000378:metabolism; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*; D013736:Testicular Neoplasms / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
chemical_list: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619380:DNMT1 protein, human; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases