title: Evaluation of binding selectivity of a polyamide probe to single base-pair different DNA in A.T-rich region by electrospray ionization mass spectrometry.
abstract: In this study, electrospray ionization mass spectrometry (ESI-MS) was used for the evaluation of the binding selectivity of a polyamide probe to single-base pair different DNA in an A.T-rich region. In this procedure, DeltaIr(dsn) was introduced as a parameter to compare the binding affinities of the polyamides with the duplex DNA. The results show that ESI-MS is a very useful tool for analysis of binding selectivity of a polyamide probe to single-base pair different DNA.
authors.affiliation: Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Li
authors.forename: Gu
authors.initials: G
authors.lastname: Yuan
mesh_terms: D020856:AT Rich Sequence; D020137:Base Pair Mismatch; D001665:Binding Sites; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry; D009757:Nylons / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D015342:DNA Probes; D009757:Nylons; D004247:DNA