title: Invader plus method detects herpes simplex virus in cerebrospinal fluid and simultaneously differentiates types 1 and 2.
abstract: We report here on the development and validation of a prototype Invader Plus method for the qualitative detection of herpes simplex virus types 1 and 2 in cerebrospinal fluid (CSF). The method combines PCR and Invader techniques in a single, closed-tube, continuous-reaction format that gives an analytical sensitivity of approximately 10 copies per reaction. The clinical sensitivity and specificity were 100.0% and 98.6%, respectively, when the results of the method were validated against the results obtained with a PCR colorimetric microtiter plate system by use of clinical CSF specimens.
authors.affiliation: Third Wave Technologies, Inc., 502 South Rosa Rd., Madison, WI 53719, USA. hallawi@twt.com
authors.forename: Hatim T
authors.initials: HT
authors.lastname: Allawi
authors.forename: Haijing
authors.initials: H
authors.lastname: Li
authors.forename: Tamara
authors.initials: T
authors.lastname: Sander
authors.forename: Azamat
authors.initials: A
authors.lastname: Aslanukov
authors.forename: Victor I
authors.initials: VI
authors.lastname: Lyamichev
authors.forename: Amondrea
authors.initials: A
authors.lastname: Blackman
authors.forename: Slava
authors.initials: S
authors.lastname: Elagin
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Tang
mesh_terms: D020805:Central Nervous System Viral Diseases / Q000175:diagnosis / Q000821:virology; D002555:Cerebrospinal Fluid / Q000821:virology*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis / Q000134:cerebrospinal fluid; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods*; D006561:Herpes Simplex / Q000175:diagnosis / Q000821:virology; D018259:Herpesvirus 1, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013937:Thymidine Kinase / Q000235:genetics
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D013937:Thymidine Kinase