Essentiality of a newly identified carbohydrate-binding module for the function of CelB (BH0603) from the alkaliphilic bacterium Bacillus halodurans.
CelB (BH0603) from Bacillus halodurans is a modular glycoside hydrolase with a family 5 catalytic module, an immunoglobulin-like module, and module PfamB of unknown function. The recombinant PfamB module bound to Avicel and was essential for CelB hydrolytic function. We propose that module PfamB be designated a new carbohydrate-binding module.
Wamalwa
Benson Munyali
BM
Applied Microbiology Laboratory, Faculty of Bioresources, Mie University, 1577 Kurimamachiyacho, Tsu 514-8507, Japan.
Sakka
Makiko
M
Shiundu
Paul Mwanza
PM
Ohmiya
Kunio
K
Kimura
Tetsuya
T
Sakka
Kazuo
K
D000468:Alkalies; D001407:Bacillus / Q000201:enzymology* / Q000378:metabolism; D050260:Carbohydrate Metabolism; D008969:Molecular Sequence Data; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*
D000468:Alkalies; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C083711:cellobiose phosphotransferase B