title: Mass spectrometry identification of circulating alpha-1-B glycoprotein, increased in aged female C57BL/6 mice.
abstract: In this study, we surveyed the profiles of mouse circulating proteins by 2-dimensional SDS-PAGE in different strains, sexes and ages. Among visible protein spots on 2-D gels with silver-staining, we identified a unique set of 7 seemingly-related proteins whose levels were consistently elevated in older C57BL/6 female mice. This set of 7 proteins was absent in C57BL/6 males or in BALB/c mice of either sex of any age. When C57BL/6 female mice were crossed with BALB/c males, the age-related increase of these proteins became sporadic and not linear in the F1 offspring. All 7 spots of this protein group were picked and subjected to identification by mass spectrometric analysis after tryptic digestion. The results showed that all 7 spots were different isoforms of alpha(1)B-glycoprotein with different degrees of post-translational modifications, such as phosphorylation. These results suggest that alpha(1)B-glycoprotein changes in mice in a sex and age dependent manner.
authors.affiliation: Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Stehle
authors.forename: Mark E
authors.initials: ME
authors.lastname: Weeks
authors.forename: Kai
authors.initials: K
authors.lastname: Lin
authors.forename: Mark C
authors.initials: MC
authors.lastname: Willingham
authors.forename: Amy M
authors.initials: AM
authors.lastname: Hicks
authors.forename: John F
authors.initials: JF
authors.lastname: Timms
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009961:Orosomucoid / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009961:Orosomucoid