A proteomics approach to identify protein expression changes in rat liver following administration of 3,5,3'-triiodo-L-thyronine.
We analyzed whole cell protein content of rat liver following T3 administration. Fourteen differentially expressed proteins were unambiguously identified and were involved in substrates and lipid metabolism, energy metabolism, detoxification of cytotoxic products, calcium homeostasis, amino acid catabolism, and the urea cycle. This study represents the first systematic identification of T3-induced changes in liver protein expression profile and provides novel information at the molecular, cellular, and tissue level of T3 action.
Silvestri
Elena
E
Dipartimento di Scienze Biologiche ed Ambientali, Università degli Studi del Sannio, Via Port'Arsa 11, 82100 Benevento, Italy.
Moreno
Maria
M
Schiavo
Luigi
L
de Lange
Pieter
P
Lombardi
Assunta
A
Chambery
Angela
A
Parente
Augusto
A
Lanni
Antonia
A
Goglia
Fernando
F
D000818:Animals; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014284:Triiodothyronine / Q000494:pharmacology*
D017931:DNA Primers; D011506:Proteins; D014284:Triiodothyronine