Dual-color split signal fluorescence in situ hybridization assays for the detection of CALM/AF10 in t(10;11)(p13;q14-q21)-positive acute leukemia.
We developed dual-color split fluorescence in situ hybridization (FISH) assays to detect AF10 and/or CALM rearrangements. Among nine cases of acute leukemia with translocation breakpoints at 10p13 and 11q14-21, a CALM/AF10 rearrangement was found in seven and was confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) in all. In 2/7 cases, FISH detected CALM/AF10 in extramedullary leukemic infiltrations in the mediastinum and breast. As expected, FISH was less sensitive than RT-PCR for disease monitoring of CALM-AF10 positive cases. This new FISH assay reliably discriminates between MLL/AF10 and CALM/AF10 genomic rearrangements, identifies variant and complex CALM/AF10 translocations and detects the CALM/AF10 rearrangement in extramedullary leukemic infiltrations.
La Starza
Roberta
R
Hematology and Bone Marrow Transplantation Unit, Policlinico Monteluce, via Brunamonti, 06122 Perugia, Italy.
Crescenzi
Barbara
B
Krause
Alexandre
A
Pierini
Valentina
V
Specchia
Giorgina
G
Bardi
Antonella
A
Nieddu
Rosa
R
Ariola
Cristina
C
Nanni
Mauro
M
Diverio
Daniela
D
Aventin
Anna
A
Sborgia
Marco
M
Martelli
Massimo F
MF
Bohlander
Stefan K
SK
Mecucci
Cristina
C
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007938:Leukemia / Q000235:genetics* / Q000473:pathology; D017254:Leukemic Infiltration / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D012189:Retrospective Studies; D014178:Translocation, Genetic
C114359:AF10-CALM fusion protein, human; D015514:Oncogene Proteins, Fusion