In situ hybridization as a tool to study the role of microRNAs in plant development.
MicroRNAs (miRNAs) have a vital role in the generation of plant forms through post-transcriptional regulation of the accumulation of developmental regulators. Analysis of their roles requires detailed knowledge of their expression patterns. We describe an in situ hybridization technique we have used to study the patterns of miRNA accumulation in Arabidopsis and in maize.
Kidner
Catherine
C
Institute of Molecular Plant Sciences, edinburgh University and Royal Botanic Gardens, Edinburgh, Scotland.
Timmermans
Marja
M
D018506:Gene Expression Regulation, Plant; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism*
D035683:MicroRNAs; D018749:RNA, Plant