MM3 potential energy surfaces of trisaccharide models of lambda-, mu-, and nu-carrageenans.
The adiabatic potential energy surfaces (PES) of six trisaccharides, sulfated derivatives of alpha-D-Gal p-(1-->3)-beta-D-Gal p-(1-->4)-alpha-D-Gal p and beta-D-Gal p-(1-->4)-alpha-D-Gal p-(1-->3)-beta-D-Gal p representing models of lambda-, mu-, and nu-carrageenans were obtained using the MM3 force-field at epsilon = 3. Each PES was described by a single contour map for which the energy is plotted against the two psi glycosidic angles, given the small variations of the phi glycosidic torsional angle in the low-energy regions of disaccharide maps. Most surfaces appear as expected from the maps of the disaccharidic repeating units of carrageenans, with less important factors altering the additive effect of both linkages. Only small interactions between the first and third monosaccharidic moieties of the trisaccharides are observed. The flexibility of the alpha-linkages appears nearly identical to that in their disaccharide counterparts, with only one exception, where it appears reduced by the presence of the third monosaccharide. On the other hand, the flexibility of the beta-linkage appears to be equal or sometimes even higher than that observed for the corresponding disaccharide.
Stortz
Carlos A
CA
Departamento de Química Orgánica-CIHIDECAR, Facultad de Ciencias Exactas y Naturales, UBA, Ciudad Universitaria, 1428 Buenos Aires, Argentina. stortz@qo.fcen.uba.ar
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002351:Carrageenan / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D013314:Stress, Mechanical; D013499:Surface Properties; D013816:Thermodynamics; D014312:Trisaccharides / Q000737:chemistry*
D014312:Trisaccharides; D002351:Carrageenan; D005690:Galactose