title: Phosphorylase coupling as a tool to convert cellobiose into amylose.
abstract: This work aims to establish the enzymatic process to produce amylose from cellobiose. Incubation of cellobiose with cellobiose phosphorylase and alpha-glucan phosphorylase in the presence of maltotetraose and a catalytic amount of inorganic phosphate at 45 degrees C for 16 h resulted in the production of linear alpha-1,4-glucan with a 19.3% (w/v, against cellobiose weight) yield. The yield was successfully improved (32.4%) when mutarotase and glucose oxidase were added to remove glucose in the reaction mixture. The weight-average molecular weight of the product was precisely controlled from 42 to 720 kDa by changing the initial molar ratio of cellobiose to maltotetraose. The combined use of two different phosphorylases should be a useful tool in converting beta-1,4-linked-polysaccharide into alpha-1,4-linked-polysaccharide.
authors.affiliation: Biochemical Research Laboratory of Ezaki Glico Co. Ltd., 4-6-5 Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan. odan-kouji@glico.co.jp
authors.forename: Kohji
authors.initials: K
authors.lastname: Ohdan
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Fujii
authors.forename: Michiyo
authors.initials: M
authors.lastname: Yanase
authors.forename: Takeshi
authors.initials: T
authors.lastname: Takaha
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuriki
mesh_terms: D000688:Amylose / Q000737:chemistry*; D002238:Carbohydrate Epimerases / Q000737:chemistry; D002475:Cellobiose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D005964:Glucosyltransferases / Q000737:chemistry*; D006005:Phosphorylases / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D011994:Recombinant Proteins; D002475:Cellobiose; D000688:Amylose; D005949:Glucose Oxidase; D005964:Glucosyltransferases; D006005:Phosphorylases; C034714:cellobiose phosphorylase; D002238:Carbohydrate Epimerases; C019646:aldose 1-epimerase