title: Topology characterization by MALDI-ToF-MS of enzymatically synthesized poly(lactide-co-glycolide).
abstract: Lipase catalyzed copolymerization of the monomers lactide and glycolide by Pseudomonas cepacia employing a molar ratio of 80L/20G has been studied. The copolymers were characterized by MALDI-ToF-MS, DSC, SEC and NMR. MALDI-ToF-MS has successfully been used not only to determine end groups and chemical composition but even the microstructure of the copolymers. We demonstrated that for this lipase catalyzed copolymerization, the main product of the reaction at 100 degrees C was linear homopolymer of lactide while at 130 degrees C the main product was cyclic random copolymer.
authors.affiliation: Laboratory of Polymer Chemistry, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
authors.forename: Saskia
authors.initials: S
authors.lastname: Huijser
authors.forename: Bastiaan B P
authors.initials: BB
authors.lastname: Staal
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.forename: Robbert
authors.initials: R
authors.lastname: Duchateau
authors.forename: Cor E
authors.initials: CE
authors.lastname: Koning
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods; D016956:Burkholderia cepacia / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D002384:Catalysis; D002412:Cations; D008049:Lipase / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D011098:Polyglactin 910 / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013696:Temperature
chemical_list: D001672:Biocompatible Materials; D002412:Cations; D046911:Macromolecular Substances; D011108:Polymers; D011098:Polyglactin 910; D008049:Lipase