Repeated intravaginal inoculation with cell-associated simian immunodeficiency virus results in persistent infection of nonhuman primates.
The goal of the present study was to develop a nonhuman primate model of intravaginal human immunodeficiency virus (HIV) transmission with cell-associated virus. Reproductively mature, cycling cynomolgus macaques with or without chemically induced, transient ulcers of the lower female reproductive tract repeatedly received challenge with a variable amount of in vitro simian immunodeficiency virus mac239-infected peripheral blood mononuclear cells. Persistent viremia was established with surprisingly few infectious lymphocytes containing physiologically relevant quantities of cell-associated virus. This model will be indispensable for the testing of vaccines and topical agents that are aimed toward the prevention of heterosexual transmission of HIV.
Kaizu
Masahiko
M
Immunogenetics and Virology Unit, Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53711, USA.
Weiler
Andrea M
AM
Weisgrau
Kim L
KL
Vielhuber
Kathleen A
KA
May
Gemma
G
Piaskowski
Shari M
SM
Furlott
Jessica
J
Maness
Nicholas J
NJ
Friedrich
Thomas C
TC
Loffredo
John T
JT
Usborne
Amy
A
Rakasz
Eva G
EG
D000282:Administration, Intravaginal; D000818:Animals; D017252:CD3 Complex / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D015658:HIV Infections / Q000503:physiopathology / Q000635:transmission* / Q000821:virology; D006801:Humans; D008252:Macaca fascicularis; D016097:Simian Acquired Immunodeficiency Syndrome / Q000503:physiopathology / Q000635:transmission* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000472:pathogenicity*; D013601:T-Lymphocytes / Q000821:virology*; D014621:Vagina / Q000821:virology*; D014766:Viremia / Q000503:physiopathology / Q000635:transmission / Q000821:virology
D017252:CD3 Complex