Hydrogen peroxide increases the phagocytic function of human neutrophils by calcium mobilisation.
We have studied the effect of exogenous administration of hydrogen peroxide (H(2)O(2)) on phagocytic activity of human neutrophils. The treatment of cells with increasing concentrations of H(2)O(2) evoke a significant elevation of phagocytic function assayed as phagocytic index, percentage and efficiency; and was similar to that induced by the calcium mobilising agonist formyl-methionyl-leucyl-phenylalanine (fMLP). This stimulatory effect was reduced by pre-treatment of neutrophils with catalase and abolished in neutrophils loaded with the intracellular calcium quelator dimethyl BAPTA. In the absence of extracellular calcium, treatment of cells with H(2)O(2) resulted in a increase in [Ca(2+)]( i ), indicating the release of calcium from intracellular stores. H(2)O(2) abolished the typical calcium release stimulated by the physiological agonist fMLP, while depletion of agonist-sensitive calcium pools by fMLP was able to prevent H(2)O(2)-induced calcium release. We conclude that H(2)O(2) induces calcium release from agonist-sensitive stores and consequently increase the phagocytosis process.
Bejarano
I
I
Department of Physiology, Faculty of Science, University of Extremadura, Av. De Elvas s/n, Badajoz 06071, Spain.
Terrón
M P
MP
Paredes
S D
SD
Barriga
C
C
Rodríguez
A B
AB
Pariente
J A
JA
D000293:Adolescent; D000328:Adult; D000368:Aged; D002118:Calcium / Q000378:metabolism*; D002614:Chelating Agents / Q000737:chemistry / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism
D002614:Chelating Agents; D016877:Oxidants; D017382:Reactive Oxygen Species; D004533:Egtazic Acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006861:Hydrogen Peroxide; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium