Simultaneous determination of 5-hydroxyindoleacetic acid and 5-hydroxytryptamine in urine samples from patients with acute appendicitis by liquid chromatography using poly(bromophenol blue) film modified electrode.
The fabrication and application of a novel electrochemical detection (ED) system with a poly(bromophenol blue) (PBPB) film chemically modified electrode (CME) for high performance liquid chromatography (HPLC) were described. The electrochemical behaviors of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) at this CME were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). It was found that the PBPB CME efficiently exhibited electrocatalytic effect on the current responses of 5-HT and 5-HIAA with relatively high sensitivity, stability and long life of activity. In HPLC-ED, the two analytes had good and stable current responses at the CME and their linear ranges were over four orders of magnitude (R> or =0.9992) with the detection limits being 0.25 nmol L(-1) for 5-HT and 0.50 nmol L(-1) for 5-HIAA. The application of this method for the determination of 5-HT and 5-HIAA in urine samples from patients with acute appendicitis (AA) was satisfactory.
Xu
Haihong
H
Department of Chemistry, East China Normal University, Shanghai 200062, PR China.
Zhang
Wen
W
Wang
Dan
D
Zhu
Wei
W
Jin
Litong
L
D000208:Acute Disease; D001064:Appendicitis / Q000652:urine*; D001978:Bromphenol Blue / Q000737:chemistry*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000652:urine*; D000432:Methanol / Q000737:chemistry; D010084:Oxidation-Reduction; D012701:Serotonin / Q000652:urine*
D001978:Bromphenol Blue; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D000432:Methanol