title: Effects of established allergen sensitization on immune and airway responses after secondary allergen sensitization.
abstract: BACKGROUND
  Spreading of sensitization with clinical manifestation of allergy is often observed in atopic individuals.
  
  
  OBJECTIVE
  To investigate the effects of an established primary allergen sensitization on immune responses and airway inflammation/reactivity on secondary allergen sensitization and airway challenges in a murine model.
  
  
  METHODS
  Balb/c mice were primarily sensitized intraperitoneally with ovalbumin or PBS, followed by systemic sensitization and airway challenges with latex extract as a secondary, unrelated allergen. Purely sham-sensitized animals were included as controls. In a second set of experiments, the primary and secondary allergens were switched.
  
  
  RESULTS
  Sensitization with ovalbumin before sensitization with latex resulted in increased production of total and latex-specific (Hev b 3-specific) IgE and IgG(1), and enhanced secretion of T(H)2-cytokines by spleen mononuclear cells cultured with mitogen compared with single latex-sensitized mice. Furthermore, airway challenges of double-sensitized mice (ovalbumin + latex) with latex caused a significant increase in airway reactivity compared with purely latex-sensitized and challenged animals. These effects were dependent on dosing and timing of the primary sensitization in relation to the secondary sensitization and independent of the primary allergen used.
  
  
  CONCLUSIONS
  Primary sensitization boosted systemic T(H)2 immune responses and enhanced the development of airway reactivity after sensitization and airway challenges with a secondary, unrelated allergen. This effect of consecutive priming was dependent on the strength of the primary sensitization but independent of the allergen used. The results explain the increased susceptibility toward sensitization spreading in atopic individuals.
  
  
  CONCLUSIONS
  Because sensitization spreading is facilitated by primary sensitization, early prevention measurements or immunotherapy should be considered at this stage of monosensitization.
authors.affiliation: Department of Pediatric Pneumology and Immunology, University Hospital Charité, Berlin. nina.bluemchen@charite.de
authors.forename: Katharina
authors.initials: K
authors.lastname: Blumchen
authors.forename: Kerstin
authors.initials: K
authors.lastname: Gerhold
authors.forename: Marcus
authors.initials: M
authors.lastname: Schwede
authors.forename: Bodo
authors.initials: B
authors.lastname: Niggemann
authors.forename: Anzhela
authors.initials: A
authors.lastname: Avagyan
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Dittrich
authors.forename: Birgit
authors.initials: B
authors.lastname: Wagner
authors.forename: Heimo
authors.initials: H
authors.lastname: Breiteneder
authors.forename: Eckard
authors.initials: E
authors.lastname: Hamelmann
mesh_terms: D000485:Allergens / Q000008:administration & dosage* / Q000276:immunology*; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000276:immunology / Q000503:physiopathology; D002478:Cells, Cultured; D005260:Female; D007117:Immunization, Secondary; D007840:Latex / Q000008:administration & dosage / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000276:immunology* / Q000503:physiopathology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000485:Allergens; D007840:Latex; D010047:Ovalbumin