title: [Relationship between anti-tumor effects of melphalan and IFN-gamma].
abstract: OBJECTIVE
  To investigate the relationship between IFN-gamma and anti-tumor effects of melphalan in vivo.
  
  
  METHODS
  Different gene-type mice (IFN-gamma(+/+), IFN-gamma(+/-) and IFN-gamma(-/-)), which had the same genetic background of C57BL/6, were used in this experiment. Murine lymphoma EL4 cells were inoculated subcutaneously into different gene-type mice simultaneously. Twelve days later, 7.5 mg/kg melphalan was used intraperitoneally to treat all these IFN-gamma(+/+), IFN-gamma(+/-) and IFN-gamma(-/-) tumor-bearing mice. Tumor size was observed and recorded every one to three days in these different gene-type mice subsequently.
  
  
  RESULTS
  After melphalan (7.5 mg/kg) treatment, tumor size decreased at a similar rate in IFN-gamma(-/-), IFN-gamma(+/-) and IFN-gamma(+/+) mice within the first 3 d. Tumors shrank further or completely disappeared in most of IFN-gamma(+/-) and IFN-gamma(+/+) mice, whereas tumors grew gradually in all IFN-gamma(-/-) mice.
  
  
  CONCLUSIONS
  7.5 mg/kg melphalan has obvious anti-tumor effects on tumor-bearing IFN-gamma(+/+) and IFN-gamma(+/-) mice, but little effects on IFN-gamma(-/-) mice. These data suggest that IFN-gamma is required for the anti-tumor effects of melphalan on tumor-bearing mice in vivo.
authors.affiliation: Pathophysiology Department of Dalian Medical University, Dalian 116027, China. molin_li@hotmail.com
authors.forename: Mo-lin
authors.initials: ML
authors.lastname: Li
authors.forename: Chuan-gang
authors.initials: CG
authors.lastname: Li
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Shu
authors.forename: Yu-jie
authors.initials: YJ
authors.lastname: Jia
authors.forename: Zhi-hai
authors.initials: ZH
authors.lastname: Qin
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D005838:Genotype; D007371:Interferon-gamma / Q000378:metabolism*; D008223:Lymphoma / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008558:Melphalan / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D008810:Mice, Inbred C57BL; D015996:Survival Rate; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D007371:Interferon-gamma; D008558:Melphalan