title: Simultaneous visualization of spatial and chromatic aberrations by two-dimensional Fourier transform spectral interferometry.
abstract: We demonstrate the use of a simple tool to simultaneously visualize and characterize chromatic and spherical aberrations that are present in multiphoton microscopy. Using two-dimensional Fourier transform spectral interferometry, we measured these aberrations, deducing in a single shot spatiotemporal effects in high-numerical-aperture objectives.
authors.affiliation: Physics, Department of Colorado School of Mines, Golden, Colorado 80402, USA. wamir@mines.edu
authors.forename: W
authors.initials: W
authors.lastname: Amir
authors.forename: T A
authors.initials: TA
authors.lastname: Planchon
authors.forename: C G
authors.initials: CG
authors.lastname: Durfee
authors.forename: J A
authors.initials: JA
authors.lastname: Squier
authors.forename: P
authors.initials: P
authors.lastname: Gabolde
authors.forename: R
authors.initials: R
authors.lastname: Trebino
authors.forename: M
authors.initials: M
authors.lastname: Müller
mesh_terms: D000465:Algorithms; D016477:Artifacts; D005583:Fourier Analysis; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007368:Interferometry / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods