title: Application of microfluidic chip with integrated optics for electrophoretic separations of proteins.
abstract: This paper describes the fabrication, the characterization and the applications of a capillary electrophoresis microchip. This hybrid device (glass/PDMS) features channels and optical waveguides integrated in one common substrate. It can be used for electrophoretic separation and fluorimetric detection of molecules. The microfluidic performance of the device is demonstrated by capillary zone and gel electrophoresis of proteins.
authors.affiliation: LEOM-ECL (UMR CNRS no. 5512), 36, av. G. de Collongue, 69134 Ecully, France. Julien.vieillard@ec-lyon.fr
authors.forename: Julien
authors.initials: J
authors.lastname: Vieillard
authors.forename: Radoslaw
authors.initials: R
authors.lastname: Mazurczyk
authors.forename: Christophe
authors.initials: C
authors.lastname: Morin
authors.forename: Benjamin
authors.initials: B
authors.lastname: Hannes
authors.forename: Yann
authors.initials: Y
authors.lastname: Chevolot
authors.forename: Paul-Louis
authors.initials: PL
authors.lastname: Desbène
authors.forename: Stanislas
authors.initials: S
authors.lastname: Krawczyk
mesh_terms: D002232:Carbocyanines / Q000302:isolation & purification; D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D055095:Optics and Photonics / Q000295:instrumentation*; D011506:Proteins / Q000302:isolation & purification*; D015203:Reproducibility of Results; D019809:Streptavidin / Q000302:isolation & purification
chemical_list: D002232:Carbocyanines; D011506:Proteins; C089187:cyanine dye 3; D019809:Streptavidin