title: Cellular target genes of Epstein-Barr virus nuclear antigen 2.
abstract: Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA-2) is a key determinant in the EBV-driven B-cell growth transformation process. By activating an array of viral and cellular target genes, EBNA-2 initiates a cascade of events which ultimately cause cell cycle entry and the proliferation of the infected B cell. In order to identify cellular target genes that respond to EBNA-2 in the absence of other viral factors, we have performed a comprehensive search for EBNA-2 target genes in two EBV-negative B-cell lines. This screen identified 311 EBNA-2-induced and 239 EBNA-2-repressed genes that were significantly regulated in either one or both cell lines. The activation of most of these genes had not previously been attributed to EBNA-2 function and will be relevant for the identification of EBNA-2-specific contributions to EBV-associated malignancies. The diverse spectrum of EBNA-2 target genes described in this study reflects the broad spectrum of EBNA-2 functions involved in virus-host interactions, including cell signaling molecules, adapters, genes involved in cell cycle regulation, and chemokines.
authors.affiliation: GSF-National Research Center for Environment and Health, Institute of Clinical Molecular Biology, Munich, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Maier
authors.forename: Gabriele
authors.initials: G
authors.lastname: Staffler
authors.forename: Andrea
authors.initials: A
authors.lastname: Hartmann
authors.forename: Julia
authors.initials: J
authors.lastname: Höck
authors.forename: Karen
authors.initials: K
authors.lastname: Henning
authors.forename: Kristina
authors.initials: K
authors.lastname: Grabusic
authors.forename: Reinhard
authors.initials: R
authors.lastname: Mailhammer
authors.forename: Reinhard
authors.initials: R
authors.lastname: Hoffmann
authors.forename: Matthias
authors.initials: M
authors.lastname: Wilmanns
authors.forename: Roland
authors.initials: R
authors.lastname: Lang
authors.forename: Jörg
authors.initials: J
authors.lastname: Mages
authors.forename: Bettina
authors.initials: B
authors.lastname: Kempkes
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D012333:RNA, Messenger; D051880:Receptors, Notch