Resistance of herpes simplex virus type 1 to acyclovir: thymidine kinase gene mutagenesis study.
By site-directed mutagenesis, we investigate the role of six mutations of herpes simplex virus type 1 thymidine kinase (TK) gene in the acquisition of resistance to acyclovir (ACV). TK activity was not impaired by substitutions located at codons 17, 161 and 374 and these mutations were thus related to TK gene polymorphism. Mutations His105Pro, Leu364Pro and Asp162Ala lead to the loss of TK activity that could result in ACV-resistance.
Frobert
Emilie
E
Laboratoire de Virologie Est, Hospices Civils de Lyon, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France. e.frobert@wanadoo.fr
Ooka
Tadamasa
T
Cortay
Jean-Claude
JC
Lina
Bruno
B
Thouvenot
Danielle
D
Morfin
Florence
F
D000212:Acyclovir / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D024882:Drug Resistance, Viral / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D006801:Humans; D016297:Mutagenesis, Site-Directed / Q000379:methods; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism
D000998:Antiviral Agents; D013937:Thymidine Kinase; D000212:Acyclovir