Ectopic ossification in the scar tissue of rats with myocardial infarction.
We describe the occurrence of bone-like formations in the left ventricular wall of infarcted rats treated or not with bone marrow cells injected systemically or locally into the myocardium. The incidence of ectopic calcification in hearts has been reported in rare cases in children with infarcts without previous coronary artery disease. Recently, ventricular calcification has been correlated with unselected bone marrow cell transplantation into infarcted rat hearts. Echocardiographic analysis of large infarction in rats frequently reveals the presence of echogenic structures in the left ventricular wall, sometimes projecting to the lumen of the chamber. The histological examination of these echogenic structures exhibited bone, cartilage, and marrow-like formations extending from the collagen-rich matrix of the ventricle wall. Microanalytical techniques verified the presence of hydroxyapatite in the mineral phase. Ossification was found in 25 out of 30 hearts evaluated 90 days postinfarct, being observed in 14 out of 17 animals submitted to cell therapy and in 11 out of 13 infarcted rats not submitted to cell therapy. Our study indicates that chondro-osteogenic differentiation can take place in the pathological rat heart independent of animal treatment with marrow cells.
Ribeiro
Karla Consort
KC
Instituto de Biofísica Carlos Chagas Filho UFRJ-CCS, Bloco G, Ilha do Fundão, Rio de Janeiro, Brasil.
Mattos
Elisabete César
EC
Werneck-de-castro
João Pedro Saar
JP
Ribeiro
Vanessa Pinho
VP
Costa-e-Sousa
Ricardo Henrique
RH
Miranda
Amarildo
A
Olivares
Emerson Lopes
EL
Farina
Marcos
M
Mill
José Geraldo
JG
Goldenberg
J Regina Coeli dos Santos
JR
Masuda
Masako Oya
MO
de Carvalho
Antônio Carlos Campos
AC
D000818:Animals; D002921:Cicatrix / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D004452:Echocardiography / Q000379:methods; D004562:Electrocardiography / Q000379:methods; D005260:Female; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D009999:Ossification, Heterotopic / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D003094:Collagen