title: First detection of the Ambler class C 1 AmpC beta-lactamase in Citrobacter freundii by a new, simple double-disk synergy test.
abstract: We report on the first detection of an AmpC-type Ambler class C 1 (ACC-1) beta-lactamase in Citrobacter freundi isolated from a patient also harboring ACC-1-producing Escherichia coli and Klebsiella pneumoniae. We propose a simple cefoxitin-based double-disk synergy test (DDST) for the specific detection of ACC-1 in members of the family Enterobacteriaceae, including natural AmpC producers, in association with a cloxacillin-based DDST as a first-line AmpC-type beta-lactamase screening test.
authors.affiliation: Université Paris VII, UFR Médicale, EA 3105, Laboratoire d'Etudes de Génétique Bactérienne dans les Infections de l'Enfant, Assistance Publique-Hôpitaux de Paris, Service de Microbiologie, Hôpital Robert Debré, Paris, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Ruppé
authors.forename: Philippe
authors.initials: P
authors.lastname: Bidet
authors.forename: Charlotte
authors.initials: C
authors.lastname: Verdet
authors.forename: Guillaume
authors.initials: G
authors.lastname: Arlet
authors.forename: Edouard
authors.initials: E
authors.lastname: Bingen
mesh_terms: D000293:Adolescent; D001426:Bacterial Proteins / Q000032:analysis*; D002440:Cefoxitin / Q000494:pharmacology; D016971:Citrobacter freundii / Q000187:drug effects / Q000201:enzymology*; D003023:Cloxacillin / Q000494:pharmacology; D003227:Conjugation, Genetic; D005243:Feces / Q000382:microbiology; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D002440:Cefoxitin; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; D003023:Cloxacillin