title: Ethnopharmacological evaluation of radal (leaves of Lomatia hirsuta) and isolation of 2-methoxyjuglone.
abstract: BACKGROUND
  Leaves of Lomatia hirsuta are used in traditional medicine in Chile under the common name of "radal". A tea of radal is traditionally used for treatment of cough, bronchial troubles, and asthma. In a preliminary screening, extracts of the leaves revealed antifungal activity, and the present phytochemical study was undertaken to explain this activity and support the traditional use.
  
  
  METHODS
  Along with the traditional tea, extracts of the leaves were screened for antifungal and toxic activities. The profile of secondary constituents was obtained using GC-MS.
  
  
  RESULTS
  2-Methoxyjuglone was isolated from the leaves of Lomatia hirsuta and found to be active against the pathogenic fungus Candida albicans (MIC = 8 microg/mL). Cinnamic acid and vanillic acid were identified as major constituents in the tea by GC-MS. The tea was found not to be toxic against Artemia salina.
  
  
  CONCLUSIONS
  The presence of phenolic acids with antimicrobial properties supports the traditional use of Radal, and encourages further studies.
authors.affiliation: Department of Plant Biology, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. hts@kvl.dk
authors.forename: Henrik T
authors.initials: HT
authors.lastname: Simonsen
authors.forename: Anne
authors.initials: A
authors.lastname: Adsersen
authors.forename: Louise
authors.initials: L
authors.lastname: Berthelsen
authors.forename: Søren B
authors.initials: SB
authors.lastname: Christensen
authors.forename: Alfonso
authors.initials: A
authors.lastname: Guzmán
authors.forename: Per
authors.initials: P
authors.lastname: Mølgaard
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D001156:Artemia / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects*; D002934:Cinnamates / Q000302:isolation & purification; D020834:Ethnopharmacology / Q000379:methods; D008826:Microbial Sensitivity Tests; D009285:Naphthoquinones / Q000302:isolation & purification* / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D039105:Proteaceae / Q000737:chemistry*; D013662:Tea / Q000737:chemistry; D014641:Vanillic Acid / Q000302:isolation & purification
chemical_list: D000935:Antifungal Agents; D002934:Cinnamates; D009285:Naphthoquinones; D010936:Plant Extracts; D013662:Tea; C029010:cinnamic acid; D014641:Vanillic Acid; C005134:juglone