Total antioxidant status and oxygen free radicals during hepatic regeneration.
OBJECTIVE
The damage induced by oxygen free radicals (OFRs) is caused by an imbalance of the production of versus the antioxidant defenses against OFRs.


METHODS
To understand hepatic damage induced by oxygen free radicals after hepatectomy in rats, total antioxidant status and total production of oxygen free radicals were serially measured in regeneration liver. At 1, 2, 3, 7, and 10 days after hepatectomy of Sprague-Dawley rats, blood was obtained into a capillary tube from a tail vein. Total antioxidant status and total production of oxygen free radicals were measured using the Randox kit, a colorimetric method, and the Free Radical Analytical System. We also measured the amount of malonyldialdehyde, which provides an indirect index of oxidative injury.


RESULTS
The level of malonyldialdehyde after hepatectomy was higher compared with that before hepatectomy. The level of total oxygen free radicals after hepatectomy was higher compared with that before hepatectomy. Total antioxidant status after hepatectomy was lower compared with that before hepatectomy.


CONCLUSIONS
The results suggested that the damage by OFRs to the regenerating liver was caused by increased production of OFRs and decreased antioxidant defense against OFRs.
Han
S Y
SY
Dongsan Kidney Institute and Chronic Disease Research Center, Keimyung University School of Medicine, 194 Dong San Dong, Daegu 700-712, Korea.
Chang
E J
EJ
Choi
H J
HJ
Nam
S I
SI
Lee
N H
NH
Kwak
C S
CS
Park
S B
SB
Kim
H C
HC
Mun
K C
KC
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D005609:Free Radicals; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010101:Oxygen Consumption; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*
D000975:Antioxidants; D005609:Free Radicals; D017382:Reactive Oxygen Species; D008315:Malondialdehyde