Membrane glucocorticoid receptor expression on peripheral blood mononuclear cells in patients with ankylosing spondylitis.
OBJECTIVE
To investigate the expression of membrane glucocorticoid receptors (mGCR) on peripheral blood mononuclear cells (PBMC) in patients with ankylosing spondylitis (AS).


METHODS
We used high sensitivity immunofluorescence with magnetofluorescent liposomes for the detection of mGCR on PBMC from patients with AS (n = 26) and healthy controls (n = 11).


RESULTS
The frequency of mGCR+ monocytes and B lymphocytes was significantly higher in patients with AS than in controls (monocytes 12.5 +/- 9.9% vs 4.8 +/- 1.4%, B lymphocytes 8.7 +/- 6.3% vs 4.4 +/- 3.6%). We did not find mGCR on T lymphocytes. The frequency of mGCR+ cells did not correlate with variables of AS disease activity [C-reactive protein, erythrocyte sedimentation rate, Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), BASDAI 6, or numerical rating scales].


CONCLUSIONS
mGCR are upregulated in monocytes and B lymphocytes of patients with AS. This upregulation does not correlate with the humoral or overall disease activity. mGCR are not present on T lymphocytes. Our findings may be related to the limited benefit of low-dose and the efficacy of high-dose (intravenous pulse or intraarticular) glucocorticoid treatment in AS. Drugs binding selectively to mGCR may be a new therapeutic option for AS.
Tryc
Anita B
AB
Department of Rheumatology and Clinical Immunology, Charité University Hospital, Campus Mitte, Charitéplatz 1, 10117 Berlin, Germany.
Spies
Cornelia M
CM
Schneider
Udo
U
Kunkel
Desiree
D
Berki
Timea
T
Sieper
Joachim
J
Burmester
Gerd-Rüdiger
GR
Radbruch
Andreas
A
Scheffold
Alexander
A
Buttgereit
Frank
F
D000328:Adult; D002462:Cell Membrane / Q000187:drug effects* / Q000276:immunology; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D008297:Male; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D013167:Spondylitis, Ankylosing / Q000097:blood*; D015854:Up-Regulation / Q000276:immunology*
D011965:Receptors, Glucocorticoid