Uronic acid determination by high performance liquid chromatography with postcolumn fluorescence derivatization.
To develop a fluorimetric high performance liquid chromatography (HPLC) technique for uronic acid microanalysis, a saline mobile phase and the postcolumn fluorimetric determination were combined. The detection limits of D-glucuronic, D-galacturonic and D-mannuronic acids were 7.19, 23.88 and 7.08 pmol, respectively. The proposed method was successfully applied to uronic acid microanalysis in a polysaccharide hydrolysate and a drink.
Kakita
Hirotaka
H
AIST Shikoku, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa, Japan. h-kakita@aist.go.jp
Kamishima
Hiroshi
H
Inouye
Kuniyo
K
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D005965:Glucuronates / Q000032:analysis / Q000737:chemistry; D006603:Hexuronic Acids / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D014574:Uronic Acids / Q000032:analysis* / Q000737:chemistry
D005965:Glucuronates; D006603:Hexuronic Acids; D014574:Uronic Acids; C007819:galacturonic acid; C008324:mannuronic acid; D005947:Glucose