Characterization of Edwardsiella tarda strains isolated from turbot, Psetta maxima (L.).
The biochemical, serological and molecular characteristics of a group of 21 Edwardsiella tarda strains isolated from turbot, Psetta maxima, in two different areas of Europe were analysed and compared with a total of 13 strains of this bacterial species with different geographical and host origins. All the turbot isolates were biochemically identical to the E. tarda strains included as reference. The use of different techniques including microagglutination, dot blot and Western blot of lipopolysaccharides allowed us to determine that all the turbot isolates constitute an homogeneous and distinctive serological group. Genetic analysis by randomly amplified polymorphic DNA (RAPD) analysis demonstrated that although the E. tarda strains from turbot were compiled in a unique group using the primers P3 and P6, two clonal lineages could be detected when oligonucleotides P4 and P5 were employed.
Castro
N
N
Departamento de Microbiología y Parasitología, Facultad de Biología e Instituto de Acuicultura, Universidad de Santiago de Compostela, Santiago de Compostela, Spain.
Toranzo
A E
AE
Barja
J L
JL
Núñez
S
S
Magariños
B
B
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001431:Bacteriological Techniques / Q000379:methods; D015153:Blotting, Western; D016172:DNA Fingerprinting / Q000379:methods; D020609:Edwardsiella tarda / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary*; D005393:Fish Diseases / Q000382:microbiology*; D005412:Flatfishes; D015151:Immunoblotting / Q000379:methods; D008070:Lipopolysaccharides / Q000276:immunology; D019081:O Antigens / Q000276:immunology; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens