Fe(III) improves antioxidant and cytoprotecting activities of mangiferin.
Iron-induced oxidative stress has been implicated in several pathological processes. In the present work we provide evidence for the formation of a mangiferin:Fe(III) complex (2:1), shown by means of either iron-induced changes in the UV/visible spectrum of mangiferin or by reduction of the anodic current peak in the voltammogram of that compound; we demonstrate, in addition, that the ferric complex is more effective than mangiferin itself in scavenging superoxide radicals generated by pyrogallol autoxidation, as well as in protecting hepatocytes from reactive oxygen species mediated hypoxia/reoxygenation injury. Moreover, we found that the mangiferin:Fe(III) complex reacts more readily with horseradish peroxidase/H(2)O(2) than does mangiferin by itself. We postulate that mangiferin could afford protection against iron/reactive oxygen species-mediated pathological processes by means of both iron chelating and iron-stimulated superoxide radical scavenging activity.
Pardo-Andreu
Gilberto L
GL
Departamento de Investigaciones Biomédicas, Centro de Química Farmacéutica, Calle 200, Esq. 21,Playa, Ciudad de La Habana, Cuba. g031071@yahoo.com <g031071@yahoo.com>
Sánchez-Baldoquín
Carlos
C
Avila-González
Rizette
R
Delgado
René
R
Naal
Zeki
Z
Curti
Carlos
C
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D004563:Electrochemistry; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D015394:Molecular Structure; D010100:Oxygen / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013481:Superoxides / Q000037:antagonists & inhibitors / Q000378:metabolism; D044004:Xanthones / Q000737:chemistry / Q000494:pharmacology*
D000975:Antioxidants; D005290:Ferric Compounds; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D044004:Xanthones; D013481:Superoxides; C013592:mangiferin; D010100:Oxygen