title: Effects of chloromethanes on growth of and deletion of the pce gene cluster in dehalorespiring Desulfitobacterium hafniense strain Y51.
abstract: The dehalorespiring Desulfitobacterium hafniense strain Y51 efficiently dechlorinates tetrachloroethene (PCE) to cis-1,2-dichloroethene (cis-DCE) via trichloroethene by PceA reductive dehalogenase encoded by the pceA gene. In a previous study, we found that the significant growth inhibition of strain Y51 occurred in the presence of commercial cis-DCE. In this study, it turned out that the growth inhibition was caused by chloroform (CF) contamination of cis-DCE. Interestingly, CF did not affect the growth of PCE-nondechlorinating SD (small deletion) and LD (large deletion) variants, where the former fails to transcribe the pceABC genes caused by a deletion of the promoter and the latter lost the entire pceABCT gene cluster. Therefore, PCE-nondechlorinating variants, mostly LD variant, became predominant, and dechlorination activity was significantly reduced in the presence of CF. Moreover, such a growth inhibitory effect was also observed in the presence of carbon tetrachloride at 1 microM, but not carbon dichloride even at 1 mM.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Taiki
authors.initials: T
authors.lastname: Futagami
authors.forename: Takehito
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Shun-Ichi
authors.initials: S
authors.lastname: Nakayama
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Goto
authors.forename: Kensuke
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D002725:Chloroform / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D045858:Desulfitobacterium / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D004000:Dichloroethylenes / Q000378:metabolism / Q000494:pharmacology; D004785:Environmental Pollutants / Q000378:metabolism / Q000494:pharmacology; D017353:Gene Deletion; D005798:Genes, Bacterial; D014644:Genetic Variation; D008737:Methyl Chloride / Q000378:metabolism / Q000494:pharmacology*; D005810:Multigene Family; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D004000:Dichloroethylenes; D004785:Environmental Pollutants; D002725:Chloroform; D008737:Methyl Chloride; D010088:Oxidoreductases; C098849:tetrachloroethene reductase; C035384:1,2-dichloroethylene