title: Effects of experimental inoculation of bovine respiratory syncytial virus in different inbred mice lineages: establishment of a murine model for BRSV infection.
abstract: Bovine respiratory syncytial virus (BRSV), a member of the subfamily Pneumovirinae, family Paramyxoviridae, is a major cause of respiratory disorders in young cattle. A number of studies were conducted to validate a reliable animal model for the infection, since BRSV inoculation on the natural host is costly and often unsuccessful. Unfortunately, after inoculation of BRSV in Balb/C mice, viral replication may be detected; however, evident pathological alterations are absent on the experimentally infected animals. In order to establish a mice model that could be used further for preliminary studies of pathological and immunological aspects of BRSV infection, three mice inbred lineages (Balb/C, A/J and C57BL6), possessing different genetic backgrounds, were tested about its susceptibility to the inoculation with BRSV. Animals were inoculated through the nasal and ocular routes and were observed after inoculation. At 7 days post-inoculation (dpi) animals were necropsied and virological (virus isolation and viral nucleic acid amplification) as well as histopathological examinations were performed. A/J and C57BL6 showed interstitial pneumonia, when compared to the Balb/C group. These findings shows that mice may constitute a suitable model for the study of BRSV infections, depending on the mice strain used for experimental inoculations.
authors.affiliation: Depto de Microbiologia e Imunologia, Instituto de Biologia, Campus UNICAMP, Campinas, SP, Brazil. spilki@unicamp.br
authors.forename: Fernando Rosado
authors.initials: FR
authors.lastname: Spilki
authors.forename: Renata Servan
authors.initials: RS
authors.lastname: Almeida
authors.forename: Helena Lage
authors.initials: HL
authors.lastname: Ferreira
authors.forename: Jacy
authors.initials: J
authors.lastname: Gameiro
authors.forename: Liana
authors.initials: L
authors.lastname: Verinaud
authors.forename: Clarice Weis
authors.initials: CW
authors.lastname: Arns
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification; D004195:Disease Models, Animal; D007150:Immunohistochemistry / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018357:Respiratory Syncytial Virus Infections / Q000662:veterinary* / Q000821:virology; D018112:Respiratory Syncytial Virus, Bovine / Q000472:pathogenicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D004279:DNA, Viral