title: Microsatellite documentation of male-mediated outcrossing between inbred laboratory strains of the self-fertilizing mangrove killifish (Kryptolebias Marmoratus).
abstract: Primers for 36 microsatellite loci were developed and employed to characterize genetic stocks and detect possible outcrossing between highly inbred laboratory strains of the self-fertilizing mangrove killifish, Kryptolebias marmoratus. From attempted crosses involving hermaphrodites from particular geographic strains and gonochoristic males from others, 2 among a total of 32 surveyed progenies (6.2%) displayed multilocus heterozygosity clearly indicative of interstrain gametic syngamy. One of these outcross hybrids was allowed to resume self-fertilization, and microsatellite assays of progeny showed that heterozygosity decreased by approximately 50% after one generation, as expected. Although populations of K. marmoratus consist mostly of synchronous hermaphrodites with efficient mechanisms of internal self-fertilization, these laboratory findings experimentally confirm that conspecific males can mediate occasional outcross events and that this process can release extensive genic heterozygosity.
authors.affiliation: Department of Genetics, University of Georgia, Athens, GA 30602, USA. mmack@uga.edu
authors.forename: Mark
authors.initials: M
authors.lastname: Mackiewicz
authors.forename: Andrey
authors.initials: A
authors.lastname: Tatarenkov
authors.forename: Andrew
authors.initials: A
authors.lastname: Perry
authors.forename: J Ryce
authors.initials: JR
authors.lastname: Martin
authors.forename: John F
authors.initials: JF
authors.lastname: Elder
authors.forename: David L
authors.initials: DL
authors.lastname: Bechler
authors.forename: John C
authors.initials: JC
authors.lastname: Avise
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D003433:Crosses, Genetic; D005306:Fertilization; D023781:Fundulidae / Q000235:genetics*; D014644:Genetic Variation; D006579:Heterozygote; D007178:Inbreeding; D008297:Male; D018895:Microsatellite Repeats