title: Distribution and determination of Pb, Cd, Bi and Cu in the sea brine system: solution--colloidal particles--biota.
abstract: The distribution of Pb, Cd, Bi, and Cu in Black Sea brine system (solution--colloidal particles--biota) produced in Burgas and Pomorie salterns is studied. The established distribution of the title elements among the brine components is as follows: Pb--25% in the salt solution, 30%--in colloidal particles, 45%--in biota (Halobacterium salinarium and microalgae Dunaliela salina); Cu--30% in the salt solution, 22%--in colloidal particles, 48%--in biota. Cd and Bi are not detected in biota. They are uniformly distributed (50%: 50%) between the salt solution and colloidal particles. Two procedures for analysis are developed. The first one is designed for determination of the total content of the studied metals in brine. It involves elimination of the biota interference by addition of ethanol, extraction and pre-concentration of the metals with NaDDC into CCl4 followed by FAAS determination. The second procedure intends determination of the elements in the separate components of the brine. It involves separation of the colloidal particles through centrifugation, separation of the studied elements from the resulting solution as dithiocarbamate complexes on a Millipore filter, dissolution of the retained metal species and subsequent FAAS analysis.
authors.affiliation: Institute of General and Inorganic Chemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria. bozhkov@svr.igic.bas.bg
authors.forename: Ognyan
authors.initials: O
authors.lastname: Bozhkov
authors.forename: Christina
authors.initials: C
authors.lastname: Tzvetkova
authors.forename: Elena
authors.initials: E
authors.lastname: Russeva
mesh_terms: D044822:Biodiversity; D001729:Bismuth / Q000032:analysis; D002104:Cadmium / Q000032:analysis; D002251:Carbon Tetrachloride / Q000737:chemistry; D000460:Chlorophyta / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D003300:Copper / Q000032:analysis; D004492:Edetic Acid / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D019015:Geologic Sediments / Q000032:analysis*; D019617:Halobacterium salinarum / Q000737:chemistry; D007854:Lead / Q000032:analysis; D019216:Metals, Heavy / Q000032:analysis*; D009792:Oceans and Seas; D010316:Particle Size; D012492:Salts / Q000032:analysis* / Q000737:chemistry; D013057:Spectrum Analysis; D013859:Thiocarbamates / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D003102:Colloids; D019216:Metals, Heavy; D012492:Salts; D013859:Thiocarbamates; D014874:Water Pollutants, Chemical; C017082:brine; D002104:Cadmium; D007854:Lead; D000431:Ethanol; D003300:Copper; D004492:Edetic Acid; D002251:Carbon Tetrachloride; D001729:Bismuth