title: A standard protocol for obtaining fish chromosomes under post-mortem conditions.
abstract: In order to improve cytogenetical analyses on fish, especially focusing on delicate and rare species, we have adopted a new in vitro methodology using dead animals. The results obtained from 24 neotropical freshwater and marine fish species demonstrate that chromosomes can be obtained under post-mortem conditions. Significantly, the samples analyzed provided reliable cytogenetical data in nearly all cases. Other advantages of this new methodology are also discussed.
authors.affiliation: Laboratório de Biologia e Genética de Peixes, Instituto de Biociências, Universidade Estadual Paulista, 18 618-000 Botucatu, SP, Brazil.
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: de Cáscia Barreto Netto
authors.forename: Erica
authors.initials: E
authors.lastname: Pauls
authors.forename: Paulo Roberto A
authors.initials: PR
authors.lastname: de Mello Affonso
mesh_terms: D000818:Animals; D002875:Chromosomes; D020732:Cytogenetic Analysis / Q000379:methods* / Q000592:standards*; D005399:Fishes / Q000235:genetics*; D008853:Microscopy