[In vivo transduction of EGFP into female mouse reproductive system by electroporation and microbubble-enhanced sonoporation].
In the present study, the efficiency of in vivo transduction of EGFP plasmid DNA into adult female mouse reproductive system by means of electroporation and microbubble-enhanced sonoporation are discussed. In the first experiment,EGFP plasmid DNA was injected into the ovary and following electroporation (square electric pulses were applied five times at 60V with a constant time of 20ms). Green fluorescence was detected 10h after transduction. In the second experiment, plasmid DNA was mixed with the microbubble at different ratio and injected into the uterus and the ovary separately. The tissues were exposed to different combined ultrasound parameters immediately after injection. We found that the suitable parameter was 2 w/cm2 intensity, 20% duty cycle and 5min. Under this condition, green fluorescence was expressed in the uterus endometrium and ovarian cortex 12 h post-sonoporation and the expression lasted up to 5 days.
Lei
Lei
L
Department of Histology and Embryology, Harbin Medical University, Harbin 150081. leil086@yahoo.com.cn
Liu
Zhong Hua
ZH
Ohta
Sho
S
Yamada
Gen
G
D000818:Animals; D018274:Electroporation / Q000379:methods*; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence; D008962:Models, Theoretical; D010053:Ovary / Q000378:metabolism*; D013010:Sonication / Q000379:methods*; D014162:Transfection / Q000379:methods
D049452:Green Fluorescent Proteins