Alterations in the mitochondrial proteome of adriamycin resistant MCF-7 breast cancer cells.
A 2D-gel based comparative proteomic analysis has been conducted of changes in mitochondrial protein abundances in MCF-7 human breast cancer cells selected for resistance to Adriamycin accompanied by verapamil. We identified and compared 156 unique proteins from 184 spots. Eleven mitochondrial proteins were found with abundances altered more than 2-fold. Transcription was evaluated for two of these, using quantitative RT-PCR. Implications of the changes are considered with respect to drug resistance.
Strong
Rachael
R
Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland, USA.
Nakanishi
Takeo
T
Ross
Douglas
D
Fenselau
Catherine
C
D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D017931:DNA Primers; D004317:Doxorubicin; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D013058:Mass Spectrometry; D008928:Mitochondria / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D011506:Proteins; D004317:Doxorubicin