Reduction in the in vitro expression of Brain-Pancreas Relative Protein by oxygen and glucose-deprivation.
Brain-Pancreas Relative Protein (BPRP) is a novel protein found in our laboratory. In previous study we observed a significant reduction in BPRP in ischemic brain of rat. Here we undertook this study to explore the possible mediating mechanism by which oxygen and glucose-deprivation culture (OGD), a model of ischemia in vitro, decreased the expression of BPRP in PC12 cells. BPRP was found to be expressed in PC12 cells and OGD caused a significant reduction in BPRP expression. The effect of OGD was primarily mediated by reactive oxygen species (ROS) because OGD upregulated the production of ROS and the inhibitors of protein kinase C, calmodulin, free radical scavengers reduced OGD-induced ROS production, while increased the expression of BPRP in PC12 cells. These data indicate that OGD decreases the expression of BPRP via enhanced formation of intracellular ROS.
Lin
Yan-Hua
YH
Department of Pharmacology, School of Basic Medical Sciences and State Key Laboratory of Natural & Biomimetic Drugs, Peking University Health Science Center, Xueyuan Road 38#, Beijing, 100083, China. xjli@bjmu.edu.cn
Liu
Ai-Hua
AH
Pan
Yan
Y
Westenbroek
Christel
C
Ter Horst
Gert J
GJ
Yu
He-Ming
HM
Li
Xue-Jun
XJ
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015687:Cell Hypoxia / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005947:Glucose / Q000172:deficiency*; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D004791:Enzyme Inhibitors; D009419:Nerve Tissue Proteins; D017382:Reactive Oxygen Species; C516848:brain-pancreas relative protein, rat; D005947:Glucose