title: Cellular autoreactivity against heat shock protein 60 in renal transplant patients: peripheral and graft-infiltrating responses.
abstract: Autoreactivity to heat shock protein 60 (Hsp60) has been implicated in the pathogenesis and regulation of chronic inflammation, especially in autoimmune diseases. In transplantation, there is a lack of information regarding the cytokine profile and specificity of cells that recognize self-Hsp60 as well as the kinetics of autoreactivity following transplantation. We studied the cellular reactivity of peripheral and graft-infiltrating lymphocytes against Hsp60 in renal transplant patients. Cytokine production induced by this protein in peripheral blood mononuclear cells indicated a predominance of interleukin (IL)-10 during the late post-transplantation period, mainly in response to intermediate and C-terminal peptides. Patients with chronic rejection presented reactivity to Hsp60 with a higher IL-10/interferon (IFN)-gamma ratio compared to long-term clinically stable patients. Graft-infiltrating T cell lines, cocultured with antigen-presenting cells, preferentially produced IL-10 after Hsp60 stimulation. These results suggest that, besides its proinflammatory activity, autoreactivity to Hsp60 in transplantation may also have a regulatory role.
authors.affiliation: Heart Institute (InCor), University of São Paulo Medical School, São Paulo, SP, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Caldas
authors.forename: E
authors.initials: E
authors.lastname: Luna
authors.forename: M
authors.initials: M
authors.lastname: Spadafora-Ferreira
authors.forename: G
authors.initials: G
authors.lastname: Porto
authors.forename: L K
authors.initials: LK
authors.lastname: Iwai
authors.forename: S E
authors.initials: SE
authors.lastname: Oshiro
authors.forename: S M
authors.initials: SM
authors.lastname: Monteiro
authors.forename: J A
authors.initials: JA
authors.lastname: Fonseca
authors.forename: F
authors.initials: F
authors.lastname: Lemos
authors.forename: J
authors.initials: J
authors.lastname: Hammer
authors.forename: P L
authors.initials: PL
authors.lastname: Ho
authors.forename: J
authors.initials: J
authors.lastname: Kalil
authors.forename: V
authors.initials: V
authors.lastname: Coelho
mesh_terms: D000293:Adolescent; D000328:Adult; D015551:Autoimmunity; D002460:Cell Line; D018834:Chaperonin 60 / Q000276:immunology*; D002648:Child; D002908:Chronic Disease; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006084:Graft Rejection / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D007668:Kidney / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology*; D008875:Middle Aged; D011184:Postoperative Period; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D018834:Chaperonin 60; D016753:Interleukin-10; D007371:Interferon-gamma