title: On-line sample extraction and enrichment of non-steroidal anti-inflammatory drugs by pre-column in capillary liquid chromatography mass spectrometry.
abstract: A rapid and sensitive analytical method has been developed for the simultaneous determination of 16 non-steroidal anti-inflammatory drugs (NSAIDs) in human plasma by capillary liquid chromatography (LC) and quadrupole mass spectrometry with electrospray ionization operated in the negative ion mode. The sample clean-up and enrichment on a pre-column were accomplished on-line to improve the sensitivity. This method greatly reduced sample preparation time and sample volume compared with off-line sample extraction methods and conventional LC methods, respectively. The recoveries of NSAIDs from human plasma were 56.7-96.9%. The total analytical time for a single analytical run was approximately 15 min. The detection limits of NSAIDs were 0.001-0.075 microg ml(-1) using a selected ion monitoring mode.
authors.affiliation: Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. c18871@pref.gifu.lg.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Suenami
authors.forename: Lee Wah
authors.initials: LW
authors.lastname: Lim
authors.forename: Toyohide
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Sasajima
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Sato
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takekoshi
authors.forename: Susumu
authors.initials: S
authors.lastname: Kanno
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal