In vitro study of lysozyme in poly(lactide-co-glycolide) microspheres with sucrose acetate isobutyrate.
This study investigated the suitability of microsphere formulations for extended protein delivery and complete protein release. These microspheres were prepared by a multi-emulsion method and prepared using a mixture of poly(lactide-co-glycolide) (PLGA), RG 502H (lactide:glycolide=50:50, M(W) 9300) and sucrose acetate isobutyrate (SAIB). SAIB embedded into the microspheres and mixed with PLGA, improved the efficiency of enzyme encapsulation. The in vitro release rate of lysozyme (Lys) from the microspheres was reduced due to the high viscosity of the added SAIB and less degradation of PLGA by SAIB. These properties enabled prolonged release of Lys for up to 2 months, characterized by a minimal initial burst of Lys and nearly zero-order protein release kinetics result from co-administration of sorbitan monooleate 80. When it is considered that degradation products of SAIB are inactive for labile proteins, SAIB may be regarded as a promising candidate for long-acting protein delivery.
Lee
Eun Seong
ES
Pharmaceutical & Health Research Institute, Amore Pacific Corporation/R&D Center, 314-1, Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-729, Republic of Korea. hejulu@hanmail.net
Kwon
Min Jung
MJ
Lee
Hyeok
H
Na
Kun
K
Kim
Jung Ju
JJ
D003692:Delayed-Action Preparations; D006016:Glycolates; D006820:Hyaluronic Acid; D019344:Lactic Acid; D008837:Micrococcus / Q000187:drug effects; D008863:Microspheres; D009113:Muramidase / Q000008:administration & dosage* / Q000494:pharmacology; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013213:Starch; D013395:Sucrose / Q000031:analogs & derivatives*; D013997:Time Factors
D003692:Delayed-Action Preparations; D006016:Glycolates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D013395:Sucrose; D006820:Hyaluronic Acid; D013213:Starch; D009113:Muramidase; C002885:sucrose acetate isobutyrate