Sperm DNA fragmentation in boars is delayed or abolished by using sperm extenders.
The semen quality of seven young adult boars was assessed for percentages of sperm motility, normal acrosomes, abnormal sperm, cells positive to sHOST (short Hipoosmotic Swelling Test), HPNA cells (sHOST Positive with Normal Acrosome cells) and the percentage of sperm heads, which exhibited DNA fragmentation using the Sperm Chromatin Dispersion test (SCD). These parameters were analysed in sperm samples both undiluted and diluted using a commercial extender and stored at 15 degrees C for 21 days. Results showed that semen quality decreases faster in the undiluted semen samples from day 0 to day 7 compared to diluted semen samples that remained with a high quality up to day 11. The undiluted semen exhibited a low DNA fragmentation index (DFI) during the first days and then a significant increase from day 7 up to day 21. This increase in the DFI coincided with the lowest levels of the other semen quality parameters. On the contrary, the samples diluted in the commercial extender showed very low levels of DNA fragmentation in all boars during the preservation period. When the evolution of DNA fragmentation was analysed in the undiluted samples, differences were found among boars. These differences were not shown in the samples diluted in the extender where the basal DFI remained stable during the 21 days. The main conclusion of this study was that some sperm extenders delay or partially prevent sperm DNA fragmentation.
Pérez-Llano
Begoña
B
Gestión Veterinaria Porcina S.L. C/Calibre 121 Pol.Ind.P-29, C.Villalba, 28400 Madrid, Spain.
Enciso
María
M
García-Casado
Pedro
P
Sala
Rubén
R
Gosálvez
Jaime
J
D000177:Acrosome / Q000502:physiology; D000818:Animals; D003080:Cold Temperature; D004249:DNA Damage; D053938:DNA Fragmentation / Q000187:drug effects*; D004530:Egg Yolk; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000002:abnormalities / Q000187:drug effects* / Q000502:physiology; D013552:Swine / Q000502:physiology*; D013997:Time Factors