Detection, characterization and identification of crucifer phytoalexins using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry.
We have analyzed 23 crucifer phytoalexins (e.g. brassinin, dioxibrassinin, cyclobrassinin, brassicanals A and C) by HPLC with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) using both negative and positive ion modes. Positive ion mode ESI-MS appeared more sensitive than negative ion mode ESI-MS in detecting this group of compounds. A new HPLC separation method, new LC-MS and LC-MS(2) data and proposed fragmentation pathways, LC retention times, and UV spectra for selected compounds are reported.
Pedras
M Soledade C
MS
Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, SK S7N 5C9, Canada. s.pedras@usask.ca
Adio
Adewale M
AM
Suchy
Mojmir
M
Okinyo
Denis P O
DP
Zheng
Qing-An
QA
Jha
Mukund
M
Sarwar
Mohammed G
MG
D019607:Brassicaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012717:Sesquiterpenes; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013729:Terpenes / Q000032:analysis* / Q000737:chemistry; D000094162:Phytoalexins
D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins