Expression of transcription factor Yin Yang 1 in human osteosarcomas.
The transcription factor Yin Yang 1 (YY1) is known to be present in some human cancer cell lines and its expression correlates with immune-mediated apoptosis. By using Western blot analysis, we have shown that the YY1 protein is strongly expressed in human osteosarcoma cells and localised mainly in the nucleus. Moreover, by using immunohistochemistry and RT-PCR techniques, we have analysed the expression of YY1 protein in biopsies from human osteosarcomas. The YY1 protein was not detectable by immunohistochemistry in osteoid tissue. However, its expression was restricted to osteosarcoma tissues. These data were confirmed by densitometric analysis of RT-PCR for YY1 expression. Thus, YY1 gene activation appears to be an early event in the process of osteoblastic transformation and its detection may represent, together with the analysis of other established markers, a useful diagnostic tool in human osteosarcomas.
de Nigris
Filomena
F
Department of General Pathology, Division of Clinical Pathology, 1st School of Medicine, II University of Naples, Italy.
Botti
Chiara
C
de Chiara
Anna
A
Rossiello
Raffaele
R
Apice
Gaetano
G
Fazioli
Flavio
F
Fiorito
Carmela
C
Sica
Vincenzo
V
Napoli
Claudio
C
D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050986:YY1 Transcription Factor / Q000378:metabolism*
D009363:Neoplasm Proteins; D050986:YY1 Transcription Factor