Porcine pregnancy-associated glycoprotein family (pPAGs) - as in vitro-produced chorionic ligands for luteal and uterine gonadotropin receptors.
This paper compares proteomic interaction-types and binding-effectiveness of secretory chorionic ligands (including pPAGs) with other proteins, i.e. gonadotropin membrane receptors (Rc) isolated from luteal-phase corpora lutea, uterine myometrium and endometrium of cyclic (cCLRc, cMYORc and cENDRc) or pregnant (pCLRc, pMYORc and pENDRc) pigs. Binding-effectiveness of miscellaneous in vitro-produced chorionic ligands (+pPAGs) was compared by radioreceptor assay (RRA) with endometrial (END) proteins of cyclic, pseudopregnant and pregnant gilts - as negative control ligands and porcine LH and hCG - as positive control ligands. The binding-comparison suggests that the pPAGs may play an important role as potential antiluteolytic or luteoprotective chorionic-origin signals during pregnancy, according to the binding-effectiveness of secretory chorionic ligands (+pPAGs) that was relatively comparable to LH/hCG - as classical ligands competing for luteal and uterine gonadotropin receptors of cyclic and pregnant pigs.
Szafrańska
Bozena
B
Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, Poland. szafran@uwm.edu.pl
Panasiewicz
Grzegorz
G
Majewska
Marta
M
D000818:Animals; D002823:Chorion; D003338:Corpus Luteum / Q000378:metabolism; D005260:Female; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism*; D011967:Receptors, Gonadotropin / Q000378:metabolism*; D013552:Swine / Q000502:physiology*; D014599:Uterus / Q000378:metabolism
D011257:Pregnancy Proteins; D011967:Receptors, Gonadotropin