title: The cDNA sequencing project.
abstract: The Dictyostelium discoideum cDNA sequencing project started in 1995, preceding the genome sequencing project. Altogether, 14 cDNA libraries, including full-length ones, were constructed from five different stages of growth and asexual and sexual development, from which nearly 100,000 randomly chosen clones were sequenced to yield over 150,000 expressed sequence tags (ESTs). The data have been publicized online to facilitate clone distribution and collaboration using the whole clone set for microarray analyses. The EST reads were assembled to 6700 independent genes, which constitute about 55% of the total estimated Dictyostelium genes. Utilization of wet and dry resources have contributed to the understanding of the genetic system controlling the multicellular development in Dictyostelium.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki-ken, Japan.
authors.forename: Hideko
authors.initials: H
authors.lastname: Urushihara
authors.forename: Takahiro
authors.initials: T
authors.lastname: Morio
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D004023:Dictyostelium / Q000235:genetics* / Q000254:growth & development; D020224:Expressed Sequence Tags; D015723:Gene Library; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary