Determination of daunomycin in human plasma and urine by using an interference-free analysis of excitation-emission matrix fluorescence data with second-order calibration.
Daunorubicin (DNR) is a significant antineoplastic antibiotic, which is usually applied to a chemotherapy of acute lymphatic and myelogenous leukaemia. Unfortunately, cardiotoxicity research in animals has indicated that DNR is cardiotoxic. Therefore, it is important to quantify DNR in biological fluids. A new algorithm, the alternating fitting residue (AFR) method, and the traditional parallel factor analysis (PARAFAC) have been utilized to directly determine DNR in human plasma and urine. These methodologies fully exploit the second-order advantage of the employed three-way fluorescence data, allowing the analyte concentrations to be quantified even in the presence of unknown fluorescent interferents. Furthermore, in contrast to PARAFAC, more satisfactory results were gained with AFR.
Xia
A-Lin
AL
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, PR China.
Wu
Hai-Long
HL
Fang
Dong-Mei
DM
Ding
Yu-Jie
YJ
Hu
Le-Qian
LQ
Yu
Ru-Qin
RQ
D000465:Algorithms; D000903:Antibiotics, Antineoplastic / Q000032:analysis / Q000097:blood / Q000652:urine; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods; D003630:Daunorubicin / Q000032:analysis* / Q000097:blood* / Q000652:urine*; D006801:Humans; D015233:Models, Statistical; D015999:Multivariate Analysis; D004364:Pharmaceutical Preparations / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000903:Antibiotics, Antineoplastic; D004364:Pharmaceutical Preparations; D003630:Daunorubicin