Role of alpha-tocopherol on antioxidant status in liver, lung and kidney of PCB exposed male albino rats.
Polychlorinated biphenyls (PCB) are widespread, lipophilic environmental pollutants which have been identified as contaminants in almost every component of the global ecosystem including fish, wildlife, and human adipose tissue, breast milk, and serum. Several studies have shown that PCBs can cause oxidative damage to biomolecules, in the form of lipid peroxidation, modulation of antioxidant enzymes, and oxidative stress. In the present study, we have sought to investigate the effects of alpha-tocopherol (vitamin E) on antioxidant status of PCB-induced toxicity in male Wistar rats. The protective effect of alpha-tocopherol (50mg/kg body weight/day) was tested in PCB-induced toxicity in rat liver, lung, and kidney. We report here that the oral supplementation of alpha-tocopherol was found to maintain the cellular redox status by maintaining the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase accompanied with glutathione and vitamin E levels and down regulation in the levels of lipid peroxides, hydroxyl radical and hydrogen peroxides generation in PCB treated rats. Therefore, our present study demonstrates the PCB-induced deficits in antioxidant enzyme activities and increase in reactive oxygen species and lipid peroxidation levels in liver, kidney, and lung which can be overcome through simultaneous supplementation with alpha-tocopherol.
Banudevi
Sivanantham
S
Department of Endocrinology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India.
Krishnamoorthy
Gunasekaran
G
Venkataraman
Prabhu
P
Vignesh
Chandraganth
C
Aruldhas
Maria Michael
MM
Arunakaran
Jagadeesan
J
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D013956:Antithyroid Agents / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity*; D004347:Drug Interactions; D004785:Environmental Pollutants / Q000633:toxicity*; D004798:Enzymes / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D024502:alpha-Tocopherol / Q000494:pharmacology*
D000975:Antioxidants; D013956:Antithyroid Agents; D004785:Environmental Pollutants; D004798:Enzymes; D017382:Reactive Oxygen Species; D020111:Chlorodiphenyl (54% Chlorine); D005978:Glutathione; D024502:alpha-Tocopherol