title: Blimp-1 is an essential component of the genetic program controlling development of the pectoral limb bud.
abstract: Formation of paired limbs in vertebrate embryos has long been a particularly useful paradigm for the study of pattern formation. Here, we show that Blimp-1, a SET domain and zinc finger-containing transcriptional factor, plays an important role in the development of the pectoral fins of the zebrafish structures that are homologous to forelimbs of amniotes. The blimp-1 gene is expressed dynamically in the mesenchyme as well as the ectodermal cells of the early fin bud, and later, in the cells of the apical ectodermal ridge (AER) of the outgrowing fin. Consistent with this expression profile, loss of Blimp-1 activity severely impairs fin outgrowth and patterning. We present evidence that blimp-1 functions downstream of tbx5 and fgf24 and therefore is not required for the initial specification of the fin bud primordia. Subsequently, however, its function is necessary for the induction of fgf10 and sonic hedgehog in the mesenchyme. In addition, Blimp-1 activity is absolutely critical for the proper induction of gene expression in the ectoderm and establishment of the AER. Taken together, these results identify an additional layer of control in the genetic pathway that operates in the developing limb and provides novel insights into regulatory mechanisms that organize its pattern.
authors.affiliation: Institute of Molecular and Cell Biology, Proteos, 61 Biopolis Drive, 117688, Singapore.
authors.forename: Ban Chuan
authors.initials: BC
authors.lastname: Lee
authors.forename: Sudipto
authors.initials: S
authors.lastname: Roy
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D004475:Ectoderm / Q000378:metabolism / Q000502:physiology; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D018878:Limb Buds; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics*; D000074462:Positive Regulatory Domain I-Binding Factor 1; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D029961:Zebrafish Proteins; D000074462:Positive Regulatory Domain I-Binding Factor 1; C513257:prdm1a protein, zebrafish