Identification of necrophagous fly species using ISSR and SCAR markers.
Necrophagous fly is the most common insect evidence collected during a death investigation. We analyzed the DNA polymorphism among the five forensically important fly species, namely, Lucilia sericata, Aldrichina grahami, Chrysomya megacephala, Parasarcophaga crassipalpis and Musca domestica using inter simple sequence repeat (ISSR) method. Nine ISSR primers selected from 18 primers could amplify 105 clear and stable bands, of which 95 bands were polymorphic. Some primers produced completely different band pattern in different species, indicating that they can be used to identify these species. Aiming at obtaining more reliable markers that might be universally used, we started an effort to convert species-specific ISSR fragments into the sequence-characterized amplified region (SCAR) markers that can be used for the molecular diagnosis of the five species.
He
Lin
L
Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, PR China.
Wang
Sibao
S
Miao
Xuexia
X
Wu
Hong
H
Huang
Yongping
Y
D000818:Animals; D017931:DNA Primers; D004175:Diptera / Q000145:classification* / Q000235:genetics*; D044707:Forensic Sciences / Q000295:instrumentation* / Q000379:methods*; D005819:Genetic Markers; D014644:Genetic Variation / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D015203:Reproducibility of Results
D017931:DNA Primers; D005819:Genetic Markers