Purification, crystallization and preliminary X-ray crystallographic analysis of chitinase from Bacillus cereus NCTU2.
Chitinases (EC 3.2.1.14) are found in a broad range of organisms, including bacteria, fungi and higher plants, and play different roles depending on their origin. A chitinase from Bacillus cereus NCTU2 (ChiNCTU2) capable of hydrolyzing chitin as a carbon and nitrogen nutrient has been identified as a member of the family 18 glycoside hydrolases. ChiNCTU2 of molecular weight 36 kDa has been crystallized using the hanging-drop vapour-diffusion method. According to the diffraction of chitinase crystals at 1.10 A resolution, the crystal belongs to space group P2(1), with unit-cell parameters a = 50.79, b = 48.79, c = 66.87 A, beta = 99.31 degrees . Preliminary analysis indicates there is one chitinase molecule in the asymmetric unit, with a solvent content of 43.4%.
Kuo
Chueh-Yuan
CY
Life Science Group, Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
Wu
Yue-Jin
YJ
Hsieh
Yin-Cheng
YC
Guan
Hong-Hsiang
HH
Tsai
Huei-Ju
HJ
Lin
Yi-Hung
YH
Huang
Yen-Chieh
YC
Liu
Ming-Yih
MY
Li
Yaw-Kuen
YK
Chen
Chun-Jung
CJ
D001409:Bacillus cereus / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002686:Chitin / Q000737:chemistry / Q000378:metabolism; D002688:Chitinases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D006868:Hydrolysis
D001426:Bacterial Proteins; D002686:Chitin; D002688:Chitinases