title: [Effect of ulinastatin on apoptosis in ileal mucosa of rats with hemorrhagic shock].
abstract: OBJECTIVE
  To investigate the effect of ulinastatin on apoptosis in ileal mucosa of rats with hemorrhagic shock.
  
  
  METHODS
  A prospective, controlled animal study was performed. The rat model of hemorrhagic shock was replicated according to method described by Chaudry. After 60 minutes period of bleeding, rats were resuscitated by transfusion of shed blood and normal saline. A part of the animals were additionally treated with ulinastatin. The expression of tumor necrosis factor-alpha (TNF-alpha), malondialdehyde (MDA) content in serum, and expression of Bax, Bcl-2, caspase 3 protein in ileal mucosa were determined at different time points after reperfusion.
  
  
  RESULTS
  Compared with the normal saline group, the expression levels of TNF-alpha, MDA content in serum, Bax and caspase 3 protein in ileal mucosa during hemorrhagic shock after resuscitation were significantly increased, while Bcl-2 protein was markedly decreased. After fluid resuscitation, obvious increase in MDA, Bcl-2 protein, significant decrease in the level of TNF-alpha, the expression of Bax and caspase 3 protein in ileal mucosa were observed in the ulinastatin group compared with normal saline group.
  
  
  CONCLUSIONS
  Ulinastatin has protective effect on rats with hemorrhagic shock by suppressing the apoptosis in ileal mucosa.
authors.affiliation: Department of Emergency, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, Guangdong, China. jly@china_em.com
authors.forename: Long-yuan
authors.initials: LY
authors.lastname: Jiang
authors.forename: Lian-hong
authors.initials: LH
authors.lastname: Yang
authors.forename: Wa
authors.initials: W
authors.lastname: Zhong
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: He
authors.forename: Jian-xing
authors.initials: JX
authors.lastname: Chang
authors.forename: Zi-tong
authors.initials: ZT
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006023:Glycoproteins / Q000494:pharmacology*; D007082:Ileum / Q000473:pathology*; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D011446:Prospective Studies; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D019253:Proto-Oncogene Proteins c-bcl-2; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D053148:Caspase 3; C028665:urinastatin