title: Microarray expression profiling of Yersinia pestis in response to chloramphenicol.
abstract: Plague is a deadly disease caused by Yersinia pestis. Human plague can be effectively controlled by timely antibiotic administration, chloramphenicol being a drug of choice. In this study, a DNA microarray was used to investigate the gene expression profile of Y. pestis in response to chloramphenicol. Seven hundred and fifty-five genes were differentially expressed on chloramphenicol treatment: 364 genes were up-regulated and 391 were down-regulated. In addition to a large number of genes encoding unknown or unassigned functions, genes encoding the components of the translation apparatus, cell envelope and transport/binding functions were strongly represented amongst the induced genes. Genes encoding proteins involved in energy metabolism and synthesis and modification of macromolecules were strongly represented amongst the down-regulated genes. A number of heat-shock genes were also repressed. These global transcriptional changes provide an insight into the mechanisms of action of chloramphenicol against Y. pestis.
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, National Center for Biomedical Analysis, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China.
authors.forename: Jingfu
authors.initials: J
authors.lastname: Qiu
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.forename: Long
authors.initials: L
authors.lastname: Qin
authors.forename: Yanping
authors.initials: Y
authors.lastname: Han
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
authors.forename: Zongmin
authors.initials: Z
authors.lastname: DU
authors.forename: Yajun
authors.initials: Y
authors.lastname: Song
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002701:Chloramphenicol / Q000494:pharmacology*; D002876:Chromosomes, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids / Q000235:genetics; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D015010:Yersinia pestis / Q000187:drug effects / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D011500:Protein Synthesis Inhibitors; D002701:Chloramphenicol