Receptor-ligand-based specific cell adhesion on solid surfaces: hippocampal neuronal cells on bilinker functionalized glass.
Cell adhesion through binding between specific cell membrane receptors and corresponding cell-adhesion-molecule (CAM)-coated solid surfaces is examined. The morphology of surfaces at various modification steps leading to functionalization with cell-binding CAMs is characterized. In one week neuron cultures, enhanced growth on surfaces modified with neuron-binding versus astrocyte-binding CAMs is observed. However, nonspecific adhesion on a poly-D-lysine-coated positive control surface is found to be even higher. Potential reasons and further studies needed are discussed.
Lu
Siyuan
S
Department of Physics, University of Southern California, Los Angeles, California 90089-0241, USA.
Bansal
Anubhuti
A
Soussou
Walid
W
Berger
Theodore W
TW
Madhukar
Anupam
A
D000818:Animals; D032701:Biomimetics / Q000379:methods; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D005898:Glass; D006624:Hippocampus / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D009474:Neurons / Q000166:cytology* / Q000502:physiology*
D015815:Cell Adhesion Molecules; D020099:Coated Materials, Biocompatible