[Chip-based capillary electrophoresis on-line coupled with atomic fluorescence spectrometry and its application to rapid speciation analysis].
Chip-based capillary electrophoresis (chip-CE) is under rapid progress due to its high efficiency, fast separation, little sample consumption and integration. Due to the short optical path (30-50 microm), the conventional UV absorbance cannot satisfy the chip detection needs. Atomic fluorescence spectrometer (AFS) is a selective and sensitive detector for hydride-forming elements. With the strong points of low cost and easy operation, AFS is attractive as an ideal detector of chip-CE for mercury, arsenic and selenium, etc. A few important practical aspects of coupling chip-CE to AFS are critically discussed. These include the design of the chip, chip-AFS interface, gas-liquid separator, AFS atomizer, and elimination of the effect of backpressure on separation. The application of the developed chip-CE-AFS to the mercury speciation analysis is highlighted.
Li
Feng
F
Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, China.
Wang
Dong-dong
DD
Yan
Xiu-ping
XP
Su
Rong-guo
RG
Lin
Jin-ming
JM