[Influence of liposome induced gene transfection on the characteristics of human immature dendritic cells].
OBJECTIVE
To investigate the influence of liposome induced gene transfection on the phenotypic characteristics and immune function of human immature dedritic cells (imDC).


METHODS
Monocytes were isolated from human cord blood, and they were differentiated into imDC by rhGM-CSF and rhIL-4 induction. Then the morphologic observation and immune phenotypic identification were performed in im-DCs. imDCs were divided into transfection (T, with liposome transfection of pEGFP vector) and control (C, without transfection) groups. The transfection rate and expression of cell maturation marker (CD83, CD86 and HLA-DR) were determined with flow cytometry, and the proliferation of non-sensitized T lymphocyte before and after transfection was determined with allogeneic mixed leukocyte reaction (MLR).


RESULTS
im-DC derived from human cord blood cells had typical appearance and surface markers consistent to what reported in the literature. The expression rates of CD86, CD83 and LA-DR in T group were (12 +/- 6) %, (8.6 +/- 2.3) % and (71 +/- 7) %, respectively, which exhibited no difference compared with those in C group (13 +/- 6) %, (9.1 +/- 3.8) % and (72 +/- 8) %, (P > 0.05). MLR results indicated that there was no obvious change in the immune stimulation function of imDC after transfection (P > 0.05) , with stimulation index lower than 2.


CONCLUSIONS
There is no change in maturation of imDC after liposome transfection, but the transfection efficiency needs to be elevated.
Wang
Yong-quan
YQ
Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing 400038, PR China.
Peng
Yi-zhi
YZ
Wang
Qiang
Q
Wang
Yi-tao
YT
You
Bo
B
D015703:Antigens, CD / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D066298:In Vitro Techniques; D008081:Liposomes / Q000378:metabolism; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection; D000097802:CD83 Antigen
D015703:Antigens, CD; D051940:B7-2 Antigen; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D008081:Liposomes; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen