A genetic linkage map of the hermaphrodite teleost fish Sparus aurata L.
The gilthead sea bream (Sparus aurata L.) is a marine fish of great importance for fisheries and aquaculture. It has also a peculiar sex-determination system, being a protandrous hermaphrodite. Here we report the construction of a first-generation genetic linkage map for S. aurata, based on 204 microsatellite markers. Twenty-six linkage groups (LG) were found. The total map length was 1241.9 cM. The ratio between sex-specific map lengths was 1:1.2 (male:female). Comparison with a preliminary radiation hybrid (RH) map reveals a good concordance, as all markers located in a single LG are located in a single RH group, except for Ad-25 and CId-31. Comparison with the Tetraodon nigroviridis genome revealed a considerable number of evolutionary conserved regions (ECRs) between the two species. The mean size of ECRs was 182 bp (sequence identity 60-90%). Forty-one ECRs have a known chromosomal location in the pufferfish genome. Despite the limited number of anchoring points, significant syntenic relationships were found. The linkage map presented here provides a robust comparative framework for QTL analysis in S. aurata and is a step toward the identification of genetic loci involved both in the determination of economically important traits and in the individual timing of sex reversal.
Franch
Rafaella
R
Department of Public Health, University of Padova, 35121 Padova, Italy.
Louro
Bruno
B
Tsalavouta
Matina
M
Chatziplis
Dimitris
D
Tsigenopoulos
Costas S
CS
Sarropoulou
Elena
E
Antonello
Jenny
J
Magoulas
Andonis
A
Mylonas
Constantinos C
CC
Babbucci
Massimiliano
M
Patarnello
Tomaso
T
Power
Deborah M
DM
Kotoulas
Giorgos
G
Bargelloni
Luca
L
D000818:Animals; D002874:Chromosome Mapping; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D057850:Hermaphroditic Organisms; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D021541:Sea Bream / Q000235:genetics*; D019849:Sex Determination Processes / Q000235:genetics
D005819:Genetic Markers