[Serological method of rapid identification of antibodies against fungi of Candida genus].
Technology of preparation of antigenic erythrocytes diagnosticum for detection of antibodies against fungi of Candida genus in humans blood is presented. Preparation is used in reaction of passive haemagglutination destined for serological investigation of candidosis. Erythrocytes of sheep treated with formalin and tannin acid were used for preparation of diagnosticum. In the capacity of sensitin were water-soluble antigens, received by multiple (5-6) freezing-wash cleaning of Candida fungi cultures. Optimal sensitizing dose (8 mkg/ml) was defined experimentally. Investigation of blood serum of healthy people has shown, that medium titer of anti-candidosis antibodies is 38.0+/-7.9. Obtained results testify, that antigenic diagnosticum could be successfully used for serological diagnosis of diseases caused by fungus of Candida genus.
Khvingiia
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D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D002175:Candida / Q000276:immunology*; D006386:Hemagglutination Tests; D011817:Rabbits; D011933:Reagent Kits, Diagnostic
D000908:Antibodies, Fungal; D011933:Reagent Kits, Diagnostic