Swine infection with Trichinella spiralis: Comparative analysis of the mucosal intestinal and systemic immune responses.
The immune protective response developed by swine against Trichinella spiralis is not yet fully understood, particularly at the mucosal level. This study aimed to characterise intestinal immunity to T. spiralis by comparison with the systemic response in specifically pathogen-free pigs. For this purpose, the kinetics of cytokine and antibody production were assessed in the intestinal mucosa and serum of swine infected with T. spiralis for up to 60 days post-infection (dpi). An ex vivo model of jejunum mucosa culture was used to collect the supernatant as a source of antibodies (Abs). Mucosal antibodies were observed by Western blot from 15 dpi, while serum antibodies were expressed from 20 dpi. Both sources of antibodies initially recognized a 110 kDa protein, followed by the identification of 35, 43/46 and 55/59 kDa proteins. IgG1 and IgA antibodies were strongly expressed within the mucosa. The expression levels of Type 1 (IFN-gamma, IL-12), Type 2 (IL-4, IL-6), pro-inflammatory (TNF-alpha) and regulatory (IL-10, TGF-beta) cytokines were assessed by RT-PCR in the intestinal mucosa and spleen. Both IL-10 and IFN-gamma mRNA levels were increased in mucosa, whereas IL-6 and IL-12 mRNA were expressed in spleen. Taken together, these results demonstrated a mixed Type 1/Type 2 profile, the Type 2 profile being dominant in the mucosa.
Picherot
Mélanie
M
JRU BIPAR 956 (INRA, AFSSA, ENVA, UPVM), AFSSA LERPAZ, 23 avenue du Général de Gaulle, 94706 Maisons-Alfort, France.
Oswald
Isabelle P
IP
Cote
Martine
M
Noeckler
Karsten
K
Le Guerhier
Franck
F
Boireau
Pascal
P
Vallée
Isabelle
I
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000917:Antibody Formation; D016207:Cytokines / Q000096:biosynthesis*; D018928:Immunity, Mucosal; D007411:Intestinal Diseases, Parasitic / Q000276:immunology / Q000469:parasitology / Q000662:veterinary; D007413:Intestinal Mucosa / Q000276:immunology / Q000469:parasitology; D008970:Molecular Weight; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000469:parasitology; D013997:Time Factors; D017160:Trichinella spiralis / Q000254:growth & development / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*
D000909:Antibodies, Helminth; D016207:Cytokines