title: Recognition and cleavage of primary microRNA transcripts.
abstract: MicroRNAs (miRNAs) are approx 22-nucleotide (nt)-long, single-stranded, endogenous, noncoding RNAs that are widely expressed in multicellular organisms. This chapter describes methods that allow the overexpression of human miRNAs and also discusses how primary miRNAs (pri-miRNAs), the much longer precursors of mature miRNAs, are processed in human cells, as well as in vitro.
authors.affiliation: Department of Pharmacology, University of Minnesota, Minneapolis, MN, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.forename: Bryan R
authors.initials: BR
authors.lastname: Cullen
mesh_terms: D006801:Humans; D066298:In Vitro Techniques; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D034622:RNA Interference; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D012322:RNA Precursors; C517918:DROSHA protein, human; D043244:Ribonuclease III