title: PARP-1 interaction with VP1 capsid protein regulates polyomavirus early gene expression.
abstract: Poly(ADP-ribose)polymerases are involved in fundamental cellular events as well as they seem to be associated to some viral infection process. In this work, the poly(ADP-ribose)polymerase-1 (PARP-1) role in the polyomavirus life cycle has been investigated. Early viral transcription was reduced by competitive inhibitors of PARPs in Swiss 3T3 cells and almost abolished in PARP-1 knockout fibroblasts and in wild-type fibroblasts when PARP-1 was silenced by RNA interference. In vivo chromatin immunoprecipitation assays showed that poly(ADP-ribosyl)ation (poly(ADP-ribose)) facilitates the release of the capsid protein viral protein 1 (VP1) from the chromatin of infecting virions. In vitro experiments demonstrated that VP1 stimulates the enzymatic activity of PARP-1 and binds non-covalently both protein-free and PARP-1-bound poly(ADP-ribose). Our studies suggest that PARP-1 promotes the complete VP1 displacement from viral DNA favouring the viral early transcription.
authors.affiliation: Pasteur Institute-Fondazione Cenci Bolognetti, Department of Cellular Biotechnology and Hematology, University of Rome La Sapienza, Viale Regina Elena 324, 00161 Rome, Italy.
authors.forename: Mariarosaria
authors.initials: M
authors.lastname: Carbone
authors.forename: Anna
authors.initials: A
authors.lastname: Reale
authors.forename: Annarita
authors.initials: A
authors.lastname: Di Sauro
authors.forename: Olga
authors.initials: O
authors.lastname: Sthandier
authors.forename: Marie-Isabelle
authors.initials: MI
authors.lastname: Garcia
authors.forename: Rossella
authors.initials: R
authors.lastname: Maione
authors.forename: Paola
authors.initials: P
authors.lastname: Caiafa
authors.forename: Paolo
authors.initials: P
authors.lastname: Amati
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002213:Capsid / Q000378:metabolism; D036022:Capsid Proteins / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D017781:Genes, Immediate-Early / Q000235:genetics*; D051379:Mice; D008957:Models, Genetic; D000071137:Poly (ADP-Ribose) Polymerase-1; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011120:Polyomavirus / Q000235:genetics* / Q000472:pathogenicity; D011485:Protein Binding; D014158:Transcription, Genetic; D053586:Virus Internalization
chemical_list: D036022:Capsid Proteins; C062481:VP1 protein, polyomavirus; D011064:Poly Adenosine Diphosphate Ribose; C499321:Parp1 protein, mouse; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases