Differential in vitro expression of the brkA gene in Bordetella pertussis and Bordetella parapertussis clinical isolates.
In this study, we set up a real-time reverse transcriptase PCR assay to measure the relative amounts of brkA transcripts in 50 Bordetella isolates. The results suggested that brkA expression is strain dependent and its level may play a role in determining the serum resistance or susceptibility phenotype. Pertussis immunocompetent sera were unable to kill Bordetella parapertussis via complement deposition.
Stefanelli
Paola
P
Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, V. le Regina Elena 299, 00161 Rome, Italy.
Sanguinetti
Maurizio
M
Fazio
Cecilia
C
Posteraro
Brunella
B
Fadda
Giovanni
G
Mastrantonio
Paola
P
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001770:Blood Bactericidal Activity; D001885:Bordetella Infections / Q000382:microbiology*; D042483:Bordetella parapertussis / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D001886:Bordetella pertussis / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007223:Infant; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014917:Whooping Cough / Q000382:microbiology*
D001425:Bacterial Outer Membrane Proteins; C090007:BrkA protein, Bordetella pertussis