title: Study of proteins associated with the Eimeria tenella refractile body by a proteomic approach.
abstract: Refractile bodies (RB), whose function is still unknown, are specific structures of Eimeriidae parasites. In order to study their proteome, RB were purified from Eimeria tenella sporozoites by a new procedure using a reversible fixation followed by centrifugation. RB proteins were resolved by two-dimensional electrophoresis. Around 76 and 89 spots were detected on RB two-dimensional gels using gradients in the 3-10 and 4-7 range, respectively. RB proteins were located mainly between pH 5 and 7. RB gels were then compared with previously established maps of the entire sporozoite proteome. Proteins appearing in new spots were identified by mass spectrometry. Thirty protein isoforms were located in RB. Added to the already known RB proteins such as Eimepsin and SO7', the new RB proteins were defined as haloacid dehalogenase, hydrolase, subtilase, lactacte dehydrogenase or ubiquitin family proteins. The RB proteome analysis confirmed the hypothesis that this structure is a reservoir for proteins necessary to invasion but also suggests that RB have energetic and metabolic functions.
authors.affiliation: Unité de Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: de Venevelles
authors.forename: Jean
authors.initials: J
authors.lastname: François Chich
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Faigle
authors.forename: Bérengère
authors.initials: B
authors.lastname: Lombard
authors.forename: Damarys
authors.initials: D
authors.lastname: Loew
authors.forename: Pierre
authors.initials: P
authors.lastname: Péry
authors.forename: Marie
authors.initials: M
authors.lastname: Labbé
mesh_terms: D000818:Animals; D016786:Eimeria tenella / Q000737:chemistry* / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome; D040901:Proteomics / Q000379:methods; D015800:Protozoan Proteins / Q000032:analysis* / Q000302:isolation & purification; D034101:Sporozoites / Q000737:chemistry
chemical_list: D020543:Proteome; D015800:Protozoan Proteins