A cooperative beads-on-a-string approach to exceptionally stable DNA triplexes.
A poly(dT) oligomer can serve as the string on which synthetic hairpins possessing poly(dA) and poly(dT) arms connected by a hydrophobic perylene diimide linker are assembled like beads on a string. The synthetic hairpins form head-to-head dimers and trimers, respectively, in the absence of the poly(dT) string and in the presence of a string with inverted polarity at mid-strand. However, they assemble in cooperative head-to-tail fashion on normal poly(dT) oligomers.
Zheng
Yan
Y
Department of Chemistry, Northwestern University, Evanston, IL 60208-3113, USA.
Long
Hai
H
Schatz
George C
GC
Lewis
Frederick D
FD
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007094:Imides / Q000737:chemistry; D009690:Nucleic Acid Conformation; D010569:Perylene / Q000031:analogs & derivatives / Q000737:chemistry; D011061:Poly A / Q000737:chemistry*; D011071:Poly T / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D007094:Imides; C510697:perylene bisimide; C051802:triplex DNA; D011061:Poly A; D011071:Poly T; C015465:poly(dA); D010569:Perylene; D004247:DNA