title: Expression of marker genes during early ear development in medaka.
abstract: Induction of the otic placode involves a number of regulatory interactions. Early studies revealed that the induction of this program is initiated by instructive signals from the mesendoderm as well as from the adjacent hindbrain. Further investigations on the molecular level identified in zebrafish Fgf3, Fgf8, Foxi1, Pax8, Dlx3b and Dlx4b genes as key players during the induction phase. Thereafter an increasing number of genes participates in the regulatory interactions finally resulting in a highly structured sensory organ. Based on data from zebrafish we selected medaka genes with presumptive functions during early ear development for an expression analysis. In addition we isolated Foxi1 and Dlx3b gene fragments from embryonic cDNA. Altogether we screened the spatio-temporal distribution of more than 20 representative marker genes for otic development in medaka embryos, with special emphasis on the early phases. Whereas the spatial distribution of these genes is largely conserved between medaka and zebrafish, our comparative analysis revealed several differences, in particular for the timing of expression.
authors.affiliation: Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Veterinarplatz 1, A-1210 Vienna, Austria.
authors.forename: Sarah
authors.initials: S
authors.lastname: Hochmann
authors.forename: Narges
authors.initials: N
authors.lastname: Aghaallaei
authors.forename: Baubak
authors.initials: B
authors.lastname: Bajoghli
authors.forename: Daniele
authors.initials: D
authors.lastname: Soroldoni
authors.forename: Matthias
authors.initials: M
authors.lastname: Carl
authors.forename: Thomas
authors.initials: T
authors.lastname: Czerny
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D019521:Body Patterning / Q000235:genetics; D018076:DNA, Complementary; D004423:Ear / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics*; D051858:Forkhead Transcription Factors / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D018398:Homeodomain Proteins / Q000235:genetics; D009990:Oryzias / Q000196:embryology* / Q000235:genetics*; D051761:Paired Box Transcription Factors / Q000235:genetics; D010641:Phenotype; D015027:Zebrafish / Q000196:embryology / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D051858:Forkhead Transcription Factors; D018398:Homeodomain Proteins; D051761:Paired Box Transcription Factors; D029961:Zebrafish Proteins; C498152:dlx3b protein, zebrafish; C472802:foxi1 protein, zebrafish