A simple peptide mapping method by partial filling micellar electrokinetic capillary chromatography with a zwitterionic-nonionic mixed micelle.
A partial filling micellar electrokinetic capillary chromatography (PF-MEKC) method with a mixed micelle system composed of a zwitterionic surfactant named 3-(N,N-dimethylhexadecylammonium)propanesulfonate (PAPS) and a nonionic surfactant polyethylene glycol dodecyl ether (Brij 35) for peptide mapping is described. The method was demonstrated by the separation of tryptic digestion of bovine serum albumin (BSA). The optimal mixed micelle solution was 50 mM NH(4)OH-HCOOH buffer (pH 2.0) containing 32 mM PAPS and 0.6% (m/v) Brij 35. It was found that the mixed micelle system permitted a highly selective separation of the tryptic digestion. The high separation selectivity was probably due to the ion-pairing interaction between the zwitterionic surfactant molecules and the peptides.
Huang
Jing
J
Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China.
Kang
Jingwu
J
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D008823:Micelles; D010449:Peptide Mapping / Q000379:methods*; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry
D008823:Micelles; D012710:Serum Albumin, Bovine; D014357:Trypsin