title: Regulation of the transcription and replication cycle of human cytomegalovirus is insensitive to genetic elimination of the cognate NF-kappaB binding sites in the enhancer.
abstract: The role of NF-kappaB in regulating human cytomegalovirus (HCMV) replication and gene transcription remains controversial. Multiple, functional NF-kappaB response elements exist in the major immediate-early promoter (MIEP) enhancer of HCMV, suggesting a possible requirement for this transcription factor in lytic viral replication. Here we demonstrate by generating and analyzing HCMVs with alterations in the MIEP-enhancer that, although this region is essential for HCMV growth, none of the four NF-kappaB response elements contained within the enhancer are required for MIE gene expression or HCMV replication in multiple cell types. These data reveal the robustness of the regulatory network controlling the MIEP enhancer.
authors.affiliation: Institut d'Investigacions Biomèdiques August Pi i Sunyer, C/ Villarroel 170, Barcelona 08036, Spain.
authors.forename: Montse
authors.initials: M
authors.lastname: Gustems
authors.forename: Eva
authors.initials: E
authors.lastname: Borst
authors.forename: Chris A
authors.initials: CA
authors.lastname: Benedict
authors.forename: Carmen
authors.initials: C
authors.lastname: Pérez
authors.forename: Martin
authors.initials: M
authors.lastname: Messerle
authors.forename: Peter
authors.initials: P
authors.lastname: Ghazal
authors.forename: Ana
authors.initials: A
authors.lastname: Angulo
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics* / Q000502:physiology*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D017353:Gene Deletion; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016328:NF-kappa B / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*; D014779:Virus Replication
chemical_list: D016328:NF-kappa B