title: Genetic variation of populations of Citrus psorosis virus.
abstract: Citrus psorosis virus (CPsV), the type species of genus Ophiovirus, has a segmented, negative-stranded RNA genome. We examined the population structure and genetic variation of CPsV in three coding regions located in RNAs 1, 2 and 3, analysing 22 isolates from Argentina, California, Florida, Italy and Spain. Most isolates contained a predominant sequence and some minor variants. Estimations of the genetic diversity and phylogenetic clustering of isolates disclosed two populations, one comprising isolates from Spain, Italy, Florida and California and the other including the Argentinean isolates. Isolate CPV-4 (from Texas) included for comparison was distant from both groups, suggesting that it belongs to a third group. The low ratio between non-synonymous and synonymous nucleotide substitutions indicated strong selection for amino acid sequence conservation, particularly in the coat protein gene. Incongruent phylogenetic relationships in different genomic regions suggested that exchange of genomic segments may have contributed to CPsV evolution.
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias, Cra Moncada-Náquera Km 4.5, Moncada, 46113 Valencia, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Martín
authors.affiliation: CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), Argentina.|Instituto de Bioquímica y Biología Molecular (IBBM), Universidad Nacional de La Plata (UNLP), La Plata, Argentina.
authors.forename: María Laura
authors.initials: ML
authors.lastname: García
authors.affiliation: Dipartimento di Arboricoltura, Botanica e Patología Vegetale, Università degli Studi di Napoli Federico II, Portici, Napoli, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Troisi
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias, Cra Moncada-Náquera Km 4.5, Moncada, 46113 Valencia, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Rubio
authors.affiliation: Instituto de Bioquímica y Biología Molecular (IBBM), Universidad Nacional de La Plata (UNLP), La Plata, Argentina.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Legarreta
authors.affiliation: Comisión de Investigaciones Científicas de Buenos Aires, Buenos Aires, Argentina.|Instituto de Bioquímica y Biología Molecular (IBBM), Universidad Nacional de La Plata (UNLP), La Plata, Argentina.
authors.forename: Oscar
authors.initials: O
authors.lastname: Grau
authors.affiliation: Dipartimento di Arboricoltura, Botanica e Patología Vegetale, Università degli Studi di Napoli Federico II, Portici, Napoli, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Alioto
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias, Cra Moncada-Náquera Km 4.5, Moncada, 46113 Valencia, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Moreno
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias, Cra Moncada-Náquera Km 4.5, Moncada, 46113 Valencia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Guerri
mesh_terms: D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D010942:Plant Viruses / Q000235:genetics*; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D012367:RNA, Viral