Isolation of respiratory syncytial virus from nasopharyngeal aspirates stored at 20 degrees C from one to fifteen months after collection.
Cell culture isolation is used for recovering respiratory syncytial virus (RSV) from respiratory specimens. As RSV is a thermolabile virus, specimens destined for inoculation into cell culture require special transport, handling, and storage. The isolation rate of RSV from nasopharyngeal aspirates (NPA) stored at 20 20 degrees C for one to 15 months after collection was investigated. A total of 126 samples considered positive for RSV by indirect fluorescence-antibody were tested by virus isolation in HEp-2 cell culture. RSV was isolated from 47/126 specimens (37.3%). These results show that RSV may be recovered from NPA stored at 20 20 degrees C by cell culture.
Nunes
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Laboratório de Virologia, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Fortaleza, CE, 60441-750, Brasil.
Moura
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D002460:Cell Line; D015925:Cryopreservation / Q000379:methods; D005455:Fluorescent Antibody Technique; D006801:Humans; D009305:Nasopharynx / Q000821:virology*; D018357:Respiratory Syncytial Virus Infections / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D013997:Time Factors