Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction.
Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.
Luce
Amy C
AC
Department of Plant Biology, University of Georgia, Georgia 30602, USA.
Sharma
Anupma
A
Mollere
Oliver S B
OS
Wolfgruber
Thomas K
TK
Nagaki
Kiyotaka
K
Jiang
Jiming
J
Presting
Gernot G
GG
Dawe
R Kelly
RK
D002503:Centromere / Q000235:genetics*; D002843:Chromatin / Q000235:genetics*; D002874:Chromosome Mapping; D005819:Genetic Markers; D047468:Immunoprecipitation; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D003313:Zea mays / Q000235:genetics
D002843:Chromatin; D005819:Genetic Markers