title: DNA and kinetic heterogeneity during the clonal evolution of adrenocortical proliferative lesions.
abstract: Monoclonal adrenocortical lesions show inverse correlation between proliferation and apoptosis, with proliferation being the single most important criterion of malignancy in adrenal lesions. No study yet has evaluated the variability of proliferation regarding the clonal pattern and diagnosis in adrenocortical nodular hyperplasias (ACNHs), adrenocortical adenomas (ACAs), and adrenocortical carcinomas (ACCs). We studied 69 ACNHs, 64 ACAs, and 23 ACCs (World Health Organization criteria) from 156 females. Clonality HUMARA test (from microdissected DNA samples), DNA content and proliferation analysis (slide and flow cytometry), and mitotic figure (MF) counting/50 high-power fields (HPFs) were performed in the same areas. Heterogeneity was assessed by 5cER (percentage of nonoctaploid cells with DNA content exceeding 5c) and standard deviation of MF/HPF. Statistics included analysis of variance/Student t tests regarding the clonal patterns and diagnosis. Polyclonal patterns were observed in 48 of 62 informative ACNHs and 7 of 56 informative ACAs, and monoclonal in 14 of 62 ACNHs, 49 of 56 ACAs, and 21 of 21 ACCs, with all hyperdiploid lesions (14 ACCs and 13 ACAs) being monoclonal. The standard deviation of MF/HPF progressively increased in ACNH-ACA-ACC (0.048 +/- 0.076, 0.110 +/- 0.097, 0.506 +/- 0.291, respectively; P = .0023), but did not differentiate ACNH/ACA. Only tetraploid percentage (P = .0496) and 5cER (P = .0352) distinguished polyclonal (3.64 +/- 2.20 and 0.14 +/- 0.15) from monoclonal (7.25 +/- 7.52 and 1.00 +/- 1.74) benign lesions. Malignancy significantly correlated with a low diploid percentage and high tetraploid percentage. Cell kinetic heterogeneity is the hallmark of adrenocortical neoplasms: tetraploid/hypertetraploid cell accumulation characterizes monoclonal lesions (suggesting nondisjunctional mitoses), whereas heterogeneously distributed mitotic figures and decreased diploid percentage define ACCs.
authors.affiliation: Department of Pathology, University Hospital of Malaga, 29010 Malaga, Spain.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Blanes
authors.forename: Salvador J
authors.initials: SJ
authors.lastname: Diaz-Cano
mesh_terms: D000306:Adrenal Cortex Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018246:Adrenocortical Adenoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018268:Adrenocortical Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D017209:Apoptosis; D002467:Cell Nucleus / Q000235:genetics / Q000473:pathology; D049109:Cell Proliferation; D002999:Clone Cells; D004273:DNA, Neoplasm / Q000032:analysis* / Q000145:classification; D018450:Disease Progression; D005434:Flow Cytometry; D006801:Humans; D006965:Hyperplasia; D019044:Image Cytometry; D019394:Ki-67 Antigen / Q000378:metabolism; D007700:Kinetics; D008940:Mitotic Index; D011123:Polyploidy; D049951:X Chromosome Inactivation / Q000235:genetics
chemical_list: D004273:DNA, Neoplasm; D019394:Ki-67 Antigen