Polymerase chain reaction with lesion scrapping for the diagnosis of human American tegumentary leishmaniasis.
The objective of this work was to compare the polymerase chain reaction (PCR) using lesion scrapping with other conventional techniques for the diagnosis of the American tegumentary leishmaniasis (ATL). For this, patients with cutaneous lesions suspected to be ATL were studied. The DNA was amplified with the MP1L/MP3H primers. From the 156 studied patients, 79 (50.6%) presented positive parasite direct search (PD), 81 (51.9%) had positive Montenegro skin test (MST), and 90 (57.7%) presented PD and/or MST positive. The PCR was positive in all of the positive-PD patients (100% sensitivity), in 91.1% of the positive PD and/or MST patients, and in 27.3% of the patients that presented negative PD and positive MST. The PCR positivity was similar to the PD (P = 0.2482) and inferior to the MST (P = 0.0455), and to the PD/MST association (P = 0.0133). The high PCR sensitivity, and positivity in those cases where the PD was negative, highlights the importance of this technique as an auxiliary tool for the diagnosis of ATL.
Venazzi
Eneide Aparecida Sabaini
EA
Departamento de Análises Clínicas, Departamento de Bioquímica, Universidade Estadual de Maringá, Maringá, PR, Brasil.
Roberto
Andréa Claudia Bekner Silva
AC
Barbosa-Tessmann
Ione Parra
IP
Zanzarini
Paulo Donizeti
PD
Lonardoni
Maria Valdrinez Campana
MV
Silveira
Thaís Gomes Verzignassi
TG
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002648:Child; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000032:analysis*; D005260:Female; D006801:Humans; D007891:Leishmania / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012882:Skin Tests
D016054:DNA, Protozoan