Transfer of surface polymerase reaction products to a secondary platform with conservation of spatial registration.
Here, we describe a method for directly transferring very small amounts of reaction products from one surface to another. The approach is illustrated using a T4 DNA polymerase reaction to extend primers hybridized to a surface-confined DNA template. Following the extension reaction, the resulting oligonucleotide is transferred to a product surface. The important results are that (1) the spatial registration of the product is preserved after transfer; (2) the same reactant surface can be used to generate and transfer multiple iterations of products; and (3) the reaction products are biologically active after transfer.
Kim
Joohoon
J
Texas Materials Institute, Center for Nano- and Molecular Science and Technology, Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712-0165, USA.
Crooks
Richard M
RM
D001710:Biotin / Q000737:chemistry; D002232:Carbocyanines / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D003845:Deoxycytosine Nucleotides / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties; D014764:Viral Proteins / Q000737:chemistry*
D002232:Carbocyanines; D004277:DNA, Single-Stranded; D003845:Deoxycytosine Nucleotides; D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D014764:Viral Proteins; C089187:cyanine dye 3; C069983:gene 43 protein, Enterobacteria phage T4; C024107:2'-deoxycytidine 5'-triphosphate; D001710:Biotin; D019809:Streptavidin; D004259:DNA-Directed DNA Polymerase