title: The mode of bone conservation does not affect the architecture and the tensile properties of rat femurs.
abstract: The bone samples used in clinical and experimental trials must be the less damaged as possible to avoid alterations of their properties. However, the mode of storage might possibly alter the bone properties, particularly microarchitecture and strength. The aim of our study was to analyze the effects of deep-freezing and alcohol conservation techniques on the densitometric, microarchitectural and biomechanical parameters of rat femurs. The left femurs were elongated in uniaxial tension up to breakdown in order to calculate biomechanical parameters. The densitometric and microarchitectural properties of right femurs were evaluated using dual-energy X-ray absorptiometry and microcomputed tomography, respectively. Results showed no significant difference in the parameters investigated between deep-freezing, alcohol storage and fresh femurs when comparing each parameter separately. Therefore, one month storage in alcohol or deep-freezing seemed to induce no harmful effect on densitometric, microarchitectural and biomechanical parameters of rat femurs.
authors.affiliation: Laboratoire ATOSEP, Université d'Orléans, Rue de Vendôme, BP 6237, 45062 Orléans cedex 2, France. helene_beaupied@yahoo.fr
authors.forename: Hélène
authors.initials: H
authors.lastname: Beaupied
authors.forename: Aurélie
authors.initials: A
authors.lastname: Dupuis
authors.forename: Alexandre
authors.initials: A
authors.lastname: Arlettaz
authors.forename: Barbara
authors.initials: B
authors.lastname: Brunet-Imbault
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bonnet
authors.forename: Christelle
authors.initials: C
authors.lastname: Jaffré
authors.forename: Claude-Laurent
authors.initials: CL
authors.lastname: Benhamou
authors.forename: Daniel
authors.initials: D
authors.lastname: Courteix
mesh_terms: D000818:Animals; D015519:Bone Density / Q000502:physiology*; D000431:Ethanol; D005269:Femur / Q000166:cytology* / Q000502:physiology*; D005615:Freezing; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013718:Tensile Strength; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D000431:Ethanol