Effects of ethylene and 1-methylcyclopropene (1-MCP) on gene expression and activity profile of alpha-1,4-glucan-phosphorylase during banana ripening.
Starch phosphorylases are enzymes that can use starch as substrate, and they are supposed to act in both in starch synthesis and degradation. This paper reports the effects of ethylene and 1-methylcyclopropene (1-MCP) on the degradation of starch and phosphorylase activity and gene expression. The results indicate that phosphorylase activity is induced during ripening and that it is associated with the onset of starch degradation. The regulation of banana phosphorylase activity is mainly dependent on gene expression, and the absence of ethylene perception by 1-MCP had a positive effect. However, this effect can be precluded by increased levels of ethylene, both autocatalytic and exogenous.
Mainardi
Janaina Aparecida
JA
Faculdade de Ciências Farmacêuticas, Departamento de Alimentos e Nutrição Experimental, Universidade de São Paulo, 580 Butantã São Paulo, SP, Brazil CEP 05508-900.
Purgatto
Eduardo
E
Vieira
Adair
A
Bastos
Walter Arato
WA
Cordenunsi
Beatriz Rosana
BR
Oliveira do Nascimento
João Roberto
JR
Lajolo
Franco Maria
FM
D003521:Cyclopropanes / Q000494:pharmacology*; D005030:Ethylenes / Q000494:pharmacology*; D005638:Fruit / Q000201:enzymology* / Q000254:growth & development; D015870:Gene Expression / Q000187:drug effects; D028521:Musa / Q000201:enzymology* / Q000254:growth & development; D006005:Phosphorylases / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis
D003521:Cyclopropanes; D005030:Ethylenes; D012333:RNA, Messenger; C036216:ethylene; D006005:Phosphorylases; C412563:1-methylcyclopropene