Oxidizing and reducing responses of granulocytes from type 2 diabetic patients.
Oxidizing/reducing response by granulocytes and a potential correlation between reactive oxygen species generation and triglyceride, total cholesterol, low-density lipoprotein, high-density lipoprotein, body mass index, fasting plasma glucose, glycemic control (hemoglobin A(1c)), or duration of diabetes were examined in type 2 diabetic patients and in healthy subjects. An increase in both oxidizing and reducing responses was observed in cells from diabetic patients relative to normoglycemic individuals. The increase in oxidizing response was nearly 2-fold higher, whereas the antioxidant response increased by 50%. Although reactive oxygen species generation from healthy subjects was correlated with levels of low-density lipoprotein (positive correlation), high-density lipoprotein (negative correlation), and body mass index (positive correlation), no such associations were observed in diabetic subjects, suggesting either an intrinsic perturbation in the oxidant/antioxidant response or possibly due to the effect of the various medications being taken by these patients. These issues need further study.
Calsolari
Maria Regina
MR
Hospital Santa Casa de Misericórdia de Belo Horizonte, MG, Brazil.
Oliveira Medina
Leonardo
L
Cunha
Elaine Patrícia
EP
Araújo Veloso
Clara
C
Martins Chaves
Miriam
M
Bosco
Adriana
A
Nogueira-Machado
José Augusto
JA
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D008076:Cholesterol, HDL / Q000378:metabolism; D008078:Cholesterol, LDL / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles
D000975:Antioxidants; D001786:Blood Glucose; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D006442:Glycated Hemoglobin; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue