Biotyping of enterotoxigenic Staphylococcus aureus by enterotoxin gene cluster (egc) polymorphism and spa typing analyses.
Thirty-five Staphylococcus aureus strains, including 10 reference strains and 25 strains recovered from clinical specimens and food samples, were analyzed by PCR REA (restriction endonucleases analysis) of the egc operon and spa typing. Nineteen spa types and seven different egc operons, including four putative new egc variants, were revealed. In 13 strains, allelic variants of sei and/or seg were found. By an analysis of their nucleotide sequence identities, a new homogeneous cluster of a sei variant, called the sei variant, was detected in six strains. In addition, the prototype sei was shown to be more polymorphic than assumed so far. Seven strains possessed the recently described seg variant, also exhibiting several nucleotide exchanges. spa typing was more effective than REA egc grouping as a typing technique. Since, in some cases, the REA typing method was able to discriminate strains showing the same spa type, it must be considered for PCR approaches involved in diagnostic procedures and may be useful for epidemiological studies. Hence, the polyphasic approach used in this study can be reliably and advantageously applied for typing egc-positive S. aureus strains.
Blaiotta
Giuseppe
G
Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, Via Università, 10080055 Portici (Naples), Italy. blaiotta@unina.it
Fusco
Vincenzina
V
von Eiff
Christof
C
Villani
Francesco
F
Becker
Karsten
K
D000483:Alleles; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D005516:Food Microbiology; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D000091402:Prohibitins; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity
D004269:DNA, Bacterial; D004768:Enterotoxins; C000717331:PHB2 protein, human; D000091402:Prohibitins