The changes of oxidative stress and human 8-hydroxyguanine glycosylase1 gene expression in depressive patients with acute leukemia.
The results of several recent studies indicated that free radicals are involved in the biochemical mechanisms that underlie neuropsychiatric disorders. In the present study, we evaluated changes in oxidative stress and human 8-hydroxyguanine glycosylase1 gene (hOGG1) expression in depressive patients with acute leukemia. Ninety two cases were assessed using the Zung self-rating depression scale (SDS) and multiple-item questionnaires. We measured total antioxidant capacity (T-AOC) and the concentrations of reactive oxygen species (ROS), superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO) during a pre-treatment period. The steady-state expression of hOGG1 mRNA transcripts was monitored. The incidence of depression was 47.83%. There was a significant decrease in serum T-AOC and SOD concentrations in depressive patients compared to the control subjects, whereas the opposite was the case for serum concentrations of ROS, NO and MDA. Real-time polymerase chain reaction (PCR) revealed that hOGG1 mRNA expression was greater in depressive patients than in the controls. Person correlation analysis revealed that depression was correlated positively with sex, the course of the disease and hOGG1 mRNA expression; depression was correlated negatively with T-AOC. Based on these results, we conclude that the antioxidant system is impaired in leukemic patients with affective disorders. Therefore, oxidative stress may play an important role in the pathophysiology of depression.
Zhou
Fuling
F
The Department of Clinical Hematology, The Affiliated No. 2 Hospital, Xi'an JiaoTong University, The West Five Road, No. 157, Xi'an 710004, PR China. zhoufuling@163.com
Zhang
Wanggang
W
Wei
Yongchang
Y
Zhou
Dangxia
D
Su
Zhixing
Z
Meng
Xin
X
Hui
Lingyun
L
Tian
Wei
W
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D045647:DNA Glycosylases / Q000235:genetics*; D003866:Depressive Disorder / Q000209:etiology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007938:Leukemia / Q000150:complications / Q000235:genetics* / Q000523:psychology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D015999:Multivariate Analysis; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013315:Stress, Psychological / Q000209:etiology*; D013482:Superoxide Dismutase / Q000097:blood
D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human