Different transformation pathways of murine fibroblast NIH 3T3 cells by hepatitis C virus core and NS3 proteins.
The oncogenic potential of both Hepatitis C virus (HCV) core and HCV NS3 proteins has been demonstrated, but these proteins induce transformation of immortal murine fibroblasts NIH 3T3 via different pathways. As long-term expression (50-100 passages) of HCV core triggers neoplastic transformation of NIH 3T3 through crisis of growth, HCV NS3 induces transformation shortly after transfection. We explain this distinction by different effects of core and NS3 on p53-mediated transactivation: inhibition by NS3 and activation by core protein.
Smirnova
Irina S
IS
Institute of Cytology RAS, Tikhoretsky Avenue 4, 194064 St. Petersburg, Russian Federation. smir@mail.cytspb.rssi.ru <smir@mail.cytspb.rssi.ru>
Aksenov
Nikolai D
ND
Vonsky
Maksim S
MS
Isaguliants
Maria G
MG
D000818:Animals; D002461:Cell Line, Transformed; D002999:Clone Cells; D005347:Fibroblasts / Q000166:cytology*; D016193:G1 Phase; D016195:G2 Phase; D016174:Hepacivirus / Q000378:metabolism*; D051379:Mice; D008938:Mitosis; D041681:NIH 3T3 Cells; D016192:Resting Phase, Cell Cycle; D015533:Transcriptional Activation; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D014758:Viral Core Proteins / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D016159:Tumor Suppressor Protein p53; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases