Characterization of Nocardia plasmid pXT107.
Nocardia, Rhodococcus and Streptomyces, all members of the actinomycetes family, are Gram-positive eubacteria with high G+C content and able to form mycelium. We report here a newly identified plasmid pXT107 of Nocardia sp. 107, one of the smallest circular plasmids found in Nocardia. The complete nucleotide sequence of pXT107 consisted of 4335 bp with 65% G+C content, and encoded one replication extragenic palindromic (Rep) and six hypothetical proteins. The Rep, double-strand origin and single-strand origin of pXT107 resembled those of typical rolling-circle-replication plasmids, such as pNI100 of Nocardia, pRE8424 of Rhodococcus and pIJ101 of Streptomyces. The Escherichia coli-Nocardia shuttle plasmid pHAQ22, containing the rep gene of pXT107, is able to propagate in Nocardia but not in Streptomyces.
Xia
Hai-Yang
HY
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Tian
Yong-Qiang
YQ
Zhang
Ran
R
Lin
Kai-Chun
KC
Qin
Zhong-Jun
ZJ
D001483:Base Sequence; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D009615:Nocardia / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000737:chemistry* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial