title: Short report: Isolation of two vaccinia virus strains from a single bovine vaccinia outbreak in rural area from Brazil: Implications on the emergence of zoonotic orthopoxviruses.
abstract: Outbreaks of bovine vaccinia disease caused by circulation of Vaccinia virus (VACV) strains have been a common occurrence in Brazil in the recent years, being an important emergent zoonosis. During a single outbreak that took place in 2001, two genetically different VACV strains were isolated and named Guarani P1 virus (GP1V) and Guarani P2 virus (GP2V). Molecular diagnosis was done through restriction fragment length polymorphism (RFLP) of ati gene (A26L) and by sequence analysis of a group of five VACV genes including the C11R, J2R, A56R, B18R, and E3L genes. These findings confirmed the co-circulation of two different Vaccinia virus strains during the same outbreak, raising important questions about the origin, emergence, and circulation of VACV strains in Brazil.
authors.affiliation: Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. gitrindade@yahoo.com.br
authors.forename: Giliane S
authors.initials: GS
authors.lastname: Trindade
authors.forename: Zélia I P
authors.initials: ZI
authors.lastname: Lobato
authors.forename: Betânia P
authors.initials: BP
authors.lastname: Drumond
authors.forename: Juliana A
authors.initials: JA
authors.lastname: Leite
authors.forename: Ricardo C
authors.initials: RC
authors.lastname: Trigueiro
authors.forename: Maria I M C
authors.initials: MI
authors.lastname: Guedes
authors.forename: Flávio G
authors.initials: FG
authors.lastname: da Fonseca
authors.forename: João R
authors.initials: JR
authors.lastname: dos Santos
authors.forename: Cláudio A
authors.initials: CA
authors.lastname: Bonjardim
authors.forename: Paulo C P
authors.initials: PC
authors.lastname: Ferreira
authors.forename: Erna G
authors.initials: EG
authors.lastname: Kroon
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D004196:Disease Outbreaks; D010802:Phylogeny; D013045:Species Specificity; D014615:Vaccinia / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014616:Vaccinia virus / Q000145:classification / Q000302:isolation & purification*