Comparison of MS/MS methods for protein identification from 2D-PAGE.
We have compared the use of a low resolution MALDI-Ion Trap MS/MS and a high-resolution ESI-TOF-MS/MS for the analysis of spots from 2D gels. The main criteria were speed and accuracy of protein identification. The results obtained using the MALDI-MS/MS system are comparable to those from the LC-MS/MS system in terms of accuracy, but less low-level proteins are identified while the time required for the analysis is dramatically reduced.
Arrigoni
Giorgio
G
Department of Protein Technology, Lund University, Sweden.
Fernandez
Celine
C
Holm
Cecilia
C
Scigelova
Michaela
M
James
Peter
P
D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D011506:Proteins