Inhibitory effects of tilianin on the expression of inducible nitric oxide synthase in low density lipoprotein receptor deficiency mice.
We investigated the effect of tilianin upon inducible nitric oxide synthesis in the plasma of low-density lipoprotein receptor knock-out (Ldlr-/-) mice fed with high cholesterol diet and in primary peritoneal macrophages of Ldlr-/- mice. High cholesterol diet induced nitric oxide production in the plasma of Ldlr-/- mice. Tilianin reduced the level of nitric oxide (NO) in plasma from Ldlr-/- mice induced by the high cholesterol diet. Tilianin also inhibited the NO production from the primary culture of peritoneal macrophages treated with lipopolysaccharide. The inhibition of NO production was caused by the suppression of inducible nitric oxide synthase (iNOS) gene expression in peritoneal macrophages isolated from Ldlr-/- mice. Moreover, tilianin inhibited the transcriptional activation of iNOS promoter that has NF-kappaB binding element. Thus, these results provide the first evidence that tilianin inhibit iNOS expression and production of NO and may act as a potential anti-inflammatory agent.
Nam
Ki-Hoan
KH
Division of Molecular Life Sciences and Center for Cell Signaling Research, Ewha Womans University, Seoul 120-750, Korea.
Choi
Jae-Hoon
JH
Seo
Yun-Jeong
YJ
Lee
Young-Mi
YM
Won
Yong-Sung
YS
Lee
Mi-Ran
MR
Lee
Mi-Ni
MN
Park
Jong-Gil
JG
Kim
Young-Myeong
YM
Kim
Hyoung-Chin
HC
Lee
Chul-Ho
CH
Lee
Hyeong-Kyu
HK
Oh
Sei-Ryang
SR
Oh
Goo Taeg
GT
D000818:Animals; D050197:Atherosclerosis / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology*; D007249:Inflammation / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis / Q000097:blood; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011973:Receptors, LDL / Q000235:genetics*; D012850:Sinus of Valsalva / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D014018:Tissue Distribution; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D005419:Flavonoids; D006027:Glycosides; D016328:NF-kappa B; D011973:Receptors, LDL; C426884:tilianin; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; D052247:Nitric Oxide Synthase Type II