title: Temporal and topographic profiles of tissue hypoxia following transient focal cerebral ischemia in rats.
abstract: Intravascular accumulation of blood cells after brain ischemia-reperfusion can cause obstruction of cerebral blood flow and tissue hypoxia/ischemia as a consequence. In the present study, we examined temporal and topographic changes of tissue hypoxia/ischemia after occlusion of the middle cerebral artery (MCA) for 60 min in rats with immunohistochemical staining for hypoxia (2-nitroimidazole hypoxia marker: hypoxyprobe-1 adducts). Our results showed that tissue hypoxia expressed as positive staining for hypoxyprobe-1 adducts preceded neuronal degeneration. Platelets and granulocytes were detected close to the hypoxyprobe-1 adducts positive area. These results suggested that the hypoxic environment could persist even after reperfusion of MCA, because of vascular obstruction with accumulation of platelets and granulocytes.
authors.affiliation: Medicinal Biology Research Laboratories, Astellas Pharmaceutical Inc, Osaka, Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Noto
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Furuichi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishiye
authors.forename: Nobuya
authors.initials: N
authors.lastname: Matsuoka
authors.forename: Ichiro
authors.initials: I
authors.lastname: Aramori
authors.forename: Seitaro
authors.initials: S
authors.lastname: Mutoh
authors.forename: Takehiko
authors.initials: T
authors.lastname: Yanagihara
authors.forename: Noboru
authors.initials: N
authors.lastname: Manabe
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D002545:Brain Ischemia / Q000503:physiopathology*; D015971:Gene Expression Regulation, Enzymologic; D039661:Integrin beta3 / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology*; D013997:Time Factors
chemical_list: D039661:Integrin beta3; D009195:Peroxidase