High energy compound stability during experimental brain death.
The aim of this study was to examine the effect of sudden brain death (BD) on myocardial function and high energy phosphate (HEP) stores. BD was induced by cerebral vessel ligation in six swine (BD group) that were compared to six control swine. At the end of the BD period (3 hours), harvested hearts were stored at 4 degrees C. Myocardial tissue HEP were assessed by: (i) (31)P-NMR spectroscopy of left ventricle for phosphocreatine (PCr), adenosine triphosphate (ATP), inorganic phosphate (Pi) and intracellular pH (pHi), and by (ii) HPLC for ATP, ADP, and AMP levels in left ventricle biopsies. Brain death resulted in a instantaneous major increase in catecholamines (>50-fold, P < .001) and paradoxically a significant progressive decrease in the regional contractility of the left ventricle. After cardioplegia, no significant differences on HEP compounds (ATP/Pi, PCr/Pi, ATP, energetic index) or in pHi were observed between BD and control groups. These data suggest that early heart injury occurring during BD does not seem to be an ischemic phenomenon.
Ferrera
R
R
INSERM EMI-U 0226, Laboratoire de Physiologie, Faculté de médecine Lyon-Nord, 8 avenue Rockefeller, 69373 Lyon Cedex 08, France. ferrera@lyon.inserm.fr
Bopassa
J
J
Rodriguez
C
C
Baverel
G
G
Ovize
M
M
D000818:Animals; D001926:Brain Death; D003645:Death, Sudden; D004734:Energy Metabolism; D006321:Heart / Q000502:physiology*; D023421:Models, Animal; D010710:Phosphates / Q000378:metabolism; D013552:Swine; D020858:Tissue and Organ Harvesting
D010710:Phosphates