title: Usefulness of the luciferase reporter system to test the efficacy of siRNA.
abstract: Small interefence RNA (siRNA) has revamped the technology of gene silencing in cultured mammalian cells after its first demonstration by Tushl et al. 4 yr ago. To circumvent the cost and the inconvenience in identifying a unique siRNA duplex that can quench target gene expression, we devised a reporter-based system to test knockdown efficiency of selected siRNAs. We demonstrated that this luciferase-based siRNA testing system can be used to evaluate the knockdown efficiency of a directly transfected siRNA duplex or an siRNA expressed from a lentiviral vector.
authors.affiliation: Center for Craniofacial Molecular Biology and Graduate Program in Cranifacial Biology, School of Dentistry, University of Southern California, Los Angeles, CA, USA.
authors.forename: Fengfeng
authors.initials: F
authors.lastname: Zhuang
authors.forename: Yi-Hsin
authors.initials: YH
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D020868:Gene Silencing; D017930:Genes, Reporter; D005822:Genetic Vectors; D016086:Lentivirus; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D032446:Myoblasts / Q000378:metabolism; D010957:Plasmids; D034741:RNA, Small Interfering / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015203:Reproducibility of Results; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D008156:Luciferases