title: Surflan and oryzalin impair reproduction in the teleost medaka (Oryzias latipes).
abstract: We conducted studies to determine if the xenoestrogens Surflan and its active ingredient oryzalin, affect indices of reproductive fitness in medaka (Oryzias latipes). Oryzalin (0.5, 0.25mg/l) or Surflan (2.0 microl/l) and oryzalin (0.5mg/l) significantly increased the mean number of non-fertilized eggs produced by treated females paired with untreated males, or by untreated females paired with treated males. Oryzalin (1.0, 0.5, 0.25mg/l) and Surflan (3.8, 2.0, 1.0 microl/l) significantly affected the time to hatch of eggs from treated females paired with untreated males, and from untreated females paired with treated males. Surflan (3.8, 2.0, 1.0 microl/l) induced intersex lesions in 80-100% of males. Oryzalin-exposed males exhibited a significant increase in the incidence of necrotic spermatids and necrotic spermatogonia, while oryzalin-exposed females had significantly fewer immature oocytes and an increase in the occurrence of hyperplastic ovaries. Our results indicate that Surflan and oryzalin affect both reproduction and gonadal histology in male and female medaka.
authors.affiliation: Environmental Restoration Division, L-542, Lawrence Livermore National Laboratory, P.O. Box 808, Livermore, CA 94550, USA. hall13@llnl.gov
authors.forename: L C
authors.initials: LC
authors.lastname: Hall
authors.forename: M
authors.initials: M
authors.lastname: Okihiro
authors.forename: M L
authors.initials: ML
authors.lastname: Johnson
authors.forename: S J
authors.initials: SJ
authors.lastname: Teh
mesh_terms: D000818:Animals; D004136:Dinitrobenzenes / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006066:Gonads / Q000187:drug effects*; D006540:Herbicides / Q000633:toxicity*; D008297:Male; D009990:Oryzias; D012098:Reproduction / Q000187:drug effects*; D013424:Sulfanilamides / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D004136:Dinitrobenzenes; D006540:Herbicides; D013424:Sulfanilamides; D014874:Water Pollutants, Chemical; C012465:oryzalin