title: [Number of sputum cultures by MGIT system needed to diagnose pulmonary tuberculosis].
abstract: OBJECTIVE
  To study the number of sputum cultures by MGIT system (Becton-Dickinson) needed to diagnose pulmonary tuberculosis.
  
  
  OBJECTIVE
  Prospective study of all patients who visited our hospital, and were strongly suspected of pulmonary tuberculosis during the period from Jan. 2002 to Sept. 2003. In these patients, 3 consecutive sputum cultures were done by both MGIT-system and egg-based Ogawa medium (1 slant).
  
  
  RESULTS
  Altogether 290 cases of sputum-culture positive pulmonary tuberculosis were available for analysis. In 210 first-sputum-smear positive cases, incremental yield of 3rd sputum culture in 3 consecutive MGIT cultures was equal to or less than 1.0% and 98.1% of culture positive cases were detected by 2 consecutive MGIT cultures. In 80 first-sputum-smear negative cases, incremental yield of 3rd sputum culture in 3 consecutive MGIT cultures was equal to or more than 5.0%, and 90.0% of culture positive cases were detected by 2 consecutive MGIT cultures. This detection rate was almost the same as the calculated detection rate (91.4%) by 3 consecutive Ogawa (2 slant) cultures (previous standard method).
  
  
  CONCLUSIONS
  It was suggested that 2 consecutive sputum cultures by MGIT were sufficient to detect M. tuberculosis in first-sputum-smear positive cases, but 3 consecutive sputum cultures by MGIT were relatively useful in first-sputum-smear negative cases.
authors.affiliation: Department of Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association (JATA), 3-1-24, Matsuyama, Kiyose-shi, Tokyo 204-8533, Japan. ito@jata.or.jp
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Ito
authors.forename: Akio
authors.initials: A
authors.lastname: Aono
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshiyama
authors.forename: Masako
authors.initials: M
authors.lastname: Wada
authors.forename: Hideo
authors.initials: H
authors.lastname: Ogata
mesh_terms: D003470:Culture Media; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification; D011446:Prospective Studies; D013183:Sputum / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
chemical_list: D003470:Culture Media