title: Role of IgG(T) and IgGa isotypes obtained from arachnidic antivenom to neutralize toxic activities of Loxosceles gaucho, Phoneutria nigriventer and Tityus serrulatus venoms.
abstract: The ability of IgG(T) and IgGa subclasses--isolated by liquid chromatography from equine arachnidic antivenom (AAV)-to neutralize toxic activities of Loxosceles gaucho, Phoneutria nigriventer and Tityus serrulatus venoms as well as to remove venom toxins from circulation was investigated. These subclasses showed similar antibody titers against L. gaucho, P. nigriventer and T. serrulatus venoms, and by immunoblotting few differences were observed in the recognition pattern of venom antigens. IgG(T) and IgGa neutralized 100% lethality induced by L. gaucho and 50% of P. nigriventer venom, but IgGa failed to neutralize T. serrulatus venom, in contrast to IgG(T). Both subclasses neutralized local reactions and dermonecrosis induced by L. gaucho venom in rabbits. In mice, IgG(T) and IgGa partially neutralized the edematogenic activity induced by P. nigriventer and T. serrulatus venoms, but only IgG(T) neutralized (ca. 81%) the nociceptive activity induced by T. serrulatus venom. Both subclasses failed to neutralize nociceptive activity induced by P. nigriventer venom. IgG(T) reduced the serum venom levels of animals injected with L. gaucho, P. nigriventer or T. serrulatus venoms, while IgGa solely reduced L. gaucho and P. nigriventer venoms levels. Our results demostrate that IgG(T) and IgGa subclasses neutralize toxic activities induced by P. nigriventer, T. serrulatus and L. gaucho venoms with different efficacies, as well as depurate these venoms from circulation.
authors.affiliation: Laboratory of Immunopathology, Butantan Institute, Av. Vital Brazil 1500, 05503-900 São Paulo, SP, Brazil.
authors.forename: Ana Flávia
authors.initials: AF
authors.lastname: Toro
authors.forename: Marília Brinati
authors.initials: MB
authors.lastname: Malta
authors.forename: Sabrina Lucio
authors.initials: SL
authors.lastname: Soares
authors.forename: Guilherme Casoni
authors.initials: GC
authors.lastname: Da Rocha
authors.forename: Marcela
authors.initials: M
authors.lastname: da Silva Lira
authors.forename: Thais Abbate
authors.initials: TA
authors.lastname: De Oliveira
authors.forename: Harumi Ando
authors.initials: HA
authors.lastname: Takehara
authors.forename: Mônica
authors.initials: M
authors.lastname: Lopes-Ferreira
authors.forename: Marcelo Larami
authors.initials: ML
authors.lastname: Santoro
authors.forename: Rosalvo
authors.initials: R
authors.lastname: Guidolin
authors.forename: Hisako
authors.initials: H
authors.lastname: Gondo Higashi
authors.forename: Irene
authors.initials: I
authors.lastname: Fernandes
authors.forename: Katia Cristina
authors.initials: KC
authors.lastname: Barbaro
mesh_terms: D000818:Animals; D000997:Antivenins / Q000737:chemistry / Q000494:pharmacology*; D002853:Chromatography, Liquid; D006736:Horses / Q000276:immunology; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D010727:Phosphoric Diester Hydrolases / Q000276:immunology / Q000633:toxicity; D012604:Scorpion Venoms / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000633:toxicity; D013111:Spider Venoms / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000633:toxicity; D013112:Spiders / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000997:Antivenins; D007074:Immunoglobulin G; D012604:Scorpion Venoms; D013111:Spider Venoms; C055161:loxosceles venom; D010727:Phosphoric Diester Hydrolases