High-throughput identification of clinical pathogenic fungi by hybridization to an oligonucleotide microarray.
Here we report the development of an oligonucleotide microarray method that can identify fungal pathogens in a single reaction. Specific oligonucleotide probes targeted to internal transcribed spacer 2 were designed and synthesized. Fungal DNA was amplified by universal primers, and the PCR product was hybridized with the oligonucleotide microarray. A series of specific hybridization profiles corresponding to species were obtained. The 122 strains of fungal pathogens, including standard and clinically isolated strains, used to test the specificity, stability, and sensitivity of the microarray system belonged to 20 species representing 8 genera. We found that the microarray system can successfully discriminate among the fungal pathogens to the species level, with high specificity and stability. The sensitivity was 15 pg/ml of DNA. This oligonucleotide microarray system represents a rapid, simple, and reliable alternative to conventional methods of identifying common clinical fungal isolates.
Huang
Aihua
A
Department of Environment and Health, Tianjin Institute of Environment and Health, 1 Dali Road, Tianjin 300050, People's Republic of China.
Li
Jun-Wen
JW
Shen
Zhi-Qiang
ZQ
Wang
Xin-Wei
XW
Jin
Min
M
D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016533:Mycological Typing Techniques; D009181:Mycoses / Q000382:microbiology*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013997:Time Factors
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D015345:Oligonucleotide Probes