title: Evidence for intracellular spatial separation of hexokinases and fructokinases in tomato plants.
abstract: Four hexokinase (LeHXK1-4) and four fructokinase (LeFRK1-4) genes were identified in tomato plants. Previous GFP fusion studies indicate that the gene product of LeHXK3 is associated with the mitochondria while that of LeHXK4 is located within plastids. In this study we found that the enzyme encoded by the fructokinase gene LeFRK3 is also located within plastids. The presence of LeFrk3 enzyme in plastids raises the question of the origin of fructose in these organelles. The other three FRKs enzymes, LeFrk1&2&4, are located in the cytosol. Unlike LeFrk1&2&4, the two additional HXKs, LeHxk1&2, share a common membrane anchor domain and are associated with the mitochondria similar to LeHxk3. The difference in the locations of the cytoplasmic FRK and HXK isozymes suggests that glucose phosphorylation is confined to defined special intracellular localizations while fructose phosphorylation is less confined.
authors.affiliation: Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel.
authors.forename: Hila
authors.initials: H
authors.lastname: Damari-Weissler
authors.forename: Michal
authors.initials: M
authors.lastname: Kandel-Kfir
authors.forename: David
authors.initials: D
authors.lastname: Gidoni
authors.forename: Anahit
authors.initials: A
authors.lastname: Mett
authors.forename: Eddy
authors.initials: E
authors.lastname: Belausov
authors.forename: David
authors.initials: D
authors.lastname: Granot
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003600:Cytosol / Q000201:enzymology; D017931:DNA Primers; D018274:Electroporation; D005631:Fructokinases / Q000737:chemistry / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006593:Hexokinase / Q000737:chemistry / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000201:enzymology*; D008969:Molecular Sequence Data; D018087:Plastids / Q000201:enzymology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D005631:Fructokinases; D006593:Hexokinase