title: Real-time quantitative allele discrimination assay using 3' locked nucleic acid primers for detection of low-percentage mosaic mutations.
authors.affiliation: Center for Medical Genetics, Ghent University Hospital, B-9000 Ghent, Belgium.
authors.forename: Ophélia
authors.initials: O
authors.lastname: Maertens
authors.forename: Eric
authors.initials: E
authors.lastname: Legius
authors.forename: Frank
authors.initials: F
authors.lastname: Speleman
authors.forename: Ludwine
authors.initials: L
authors.lastname: Messiaen
authors.forename: Jo
authors.initials: J
authors.lastname: Vandesompele
mesh_terms: D000483:Alleles; D020137:Base Pair Mismatch / Q000235:genetics; D020029:Base Pairing; D001681:Biological Assay; D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000235:genetics / Q000379:methods; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; C477371:locked nucleic acid