title: Effect of hyperthermia on the replication of white spot syndrome virus (WSSV) in Procambarus clarkii.
abstract: The effect of hyperthermia on the development of white spot syndrome virus (WSSV) in the crayfish Procambarus clarkii was studied by competitive PCR. Crayfish were exposed to different temperatures (24 +/- 1 and 32 +/- 1 degrees C) after WSSV injection. No mortality was observed when crayfish were held at 32 +/- 1 degrees C, but mortality reached 100% when crayfish were transferred to 24 +/- 1 degrees C. Competitive PCR showed that viral levels at 32 +/- 1 degrees C remained at 10(5) copies mg(-1) tissue, while at 24 +/- 1 degrees C levels were significantly higher, rising from 10(4) to 10(10) copies mg(-1) tissue. These results suggest that hyperthermia reduces viral replication, but does not eliminate viral particles from WSSV-infected crayfish.
authors.affiliation: Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Feed Science Institute, Zhejiang University, Hangzhou, PR China.
authors.forename: Hua-Hua
authors.initials: HH
authors.lastname: Du
authors.forename: Wei-Fen
authors.initials: WF
authors.lastname: Li
authors.forename: Zi-Rong
authors.initials: ZR
authors.lastname: Xu
authors.forename: Zong-Su
authors.initials: ZS
authors.lastname: Kil
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000821:virology*; D017931:DNA Primers / Q000737:chemistry; D005880:Gills / Q000821:virology; D006358:Hot Temperature; D016133:Polymerase Chain Reaction / Q000379:methods; D016019:Survival Analysis; D013997:Time Factors; D014779:Virus Replication / Q000502:physiology*; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D017931:DNA Primers