Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning.
We report a method for the identification and quantification of glycerophospholipid molecular species that is based on the simultaneous automated acquisition and processing of 41 precursor ion spectra, specific for acyl anions of common fatty acids moieties and several lipid class-specific fragment ions. Absolute quantification of identified species was linear within a concentration range of 10 nM-100 microM and was achieved by spiking into total lipid extracts a set of synthetic lipid standards with diheptadecanoyl (17:0/17:0) fatty acid moieties, representing six common classes of glycerophospholipids. The automated analysis of total lipid extracts was powered by a robotic nanoflow ion source and produced currently the most detailed description of the glycerophospholipidome.
Ejsing
Christer S
CS
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Duchoslav
Eva
E
Sampaio
Julio
J
Simons
Kai
K
Bonner
Ron
R
Thiele
Christoph
C
Ekroos
Kim
K
Shevchenko
Andrej
A
D005227:Fatty Acids; D020404:Glycerophospholipids / Q000032:analysis* / Q000737:chemistry*; D007477:Ions / Q000737:chemistry*; D007554:Isotopes; D012985:Software Design; D013997:Time Factors
D005227:Fatty Acids; D020404:Glycerophospholipids; D007477:Ions; D007554:Isotopes