title: FISH-mapping of LEP and SLC26A2 genes in sheep, goat and cattle R-banded chromosomes: comparison between bovine, ovine and caprine chromosome 4 (BTA4/OAR4/CHI4) and human chromosome 7 (HSA7).
abstract: Sheep (OAR), goat (CHI) and cattle (BTA) R-banded chromosome preparations, obtained from synchronized cell cultures, were used to FISH-map leptin (LEP) and solute carrier family 26 member 2 (SLC26A2) genes on single chromosome bands. LEP maps on OAR4q32 and CHI4q32, being the first assignment of this gene to these two species. SLC26A2 maps on BTA7q24, OAR5q24 and CHI7q24. This gene, too, was assigned for the fist time to both sheep and goat chromosomes, while it was more precisely localized on a single chromosome band in cattle. Improved cytogenetic maps of BTA4/OAR4/CHI4 were constructed and compared with HSA7 revealing five main conserved segments and complex chromosome rearrangements, including a centromere repositioning, differentiating HSA7 and BTA4/OAR4/CHI4.
authors.affiliation: Laboratory of Animal Cytogenetics and Gene Mapping, National Research Council (CNR), ISPAAM, Naples, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Perucatti
authors.forename: G P
authors.initials: GP
authors.lastname: Di Meo
authors.forename: M
authors.initials: M
authors.lastname: Vallinoto
authors.forename: G
authors.initials: G
authors.lastname: Kierstein
authors.forename: M P C
authors.initials: MP
authors.lastname: Schneider
authors.forename: D
authors.initials: D
authors.lastname: Incarnato
authors.forename: A
authors.initials: A
authors.lastname: Caputi Jambrenghi
authors.forename: G
authors.initials: G
authors.lastname: Mohammadi
authors.forename: G
authors.initials: G
authors.lastname: Vonghia
authors.forename: A
authors.initials: A
authors.lastname: Silva
authors.forename: B
authors.initials: B
authors.lastname: Brenig
authors.forename: L
authors.initials: L
authors.lastname: Iannuzzi
mesh_terms: D000818:Animals; D027321:Anion Transport Proteins; D002417:Cattle; D002478:Cells, Cultured; D002871:Chromosome Banding; D002874:Chromosome Mapping / Q000379:methods*; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D006041:Goats; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020738:Leptin / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics; D012756:Sheep; D000076988:Sulfate Transporters
chemical_list: D027321:Anion Transport Proteins; D020738:Leptin; D026901:Membrane Transport Proteins; C089651:SLC26A2 protein, human; D000076988:Sulfate Transporters