title: Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein.
abstract: The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.
authors.affiliation: RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Sarai
authors.forename: Wataru
authors.initials: W
authors.lastname: Kagawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Kinebuchi
authors.forename: Ako
authors.initials: A
authors.lastname: Kagawa
authors.forename: Kozo
authors.initials: K
authors.lastname: Tanaka
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Miyagawa
authors.forename: Shukuko
authors.initials: S
authors.lastname: Ikawa
authors.forename: Takehiko
authors.initials: T
authors.lastname: Shibata
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kurumizaka
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D004265:DNA Helicases / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D004277:DNA, Single-Stranded / Q000378:metabolism / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D051135:Rad51 Recombinase / Q000378:metabolism; D045522:Recombinases / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D011995:Recombination, Genetic
chemical_list: D018797:Cell Cycle Proteins; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C491221:RAD54B protein, human; D045522:Recombinases; D051135:Rad51 Recombinase; D000251:Adenosine Triphosphatases; C074556:DMC1 protein, human; D004265:DNA Helicases