title: Phenolics and antimicrobial activity of traditional stoned table olives 'alcaparra'.
abstract: In the present work, we report the determination of phenolic compounds in 'alcaparra' table olives by reversed-phase HPLC/DAD, and the evaluation of their extract in vitro activity against several microorganisms that may be causal agents of human intestinal and respiratory tract infections, namely Gram positive (Bacillus cereus, Bacillus subtilis, and Staphylococcus aureus), Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae) and fungi (Candida albicans and Cryptococcus neoformans). Three flavonoidic compounds were identified and quantified: luteolin 7-O-glucoside, apigenin 7-O-glucoside, and luteolin. At low concentrations (0.05mg/mL) 'alcaparra' extract revealed significant inhibition of both Gram positive and Gram negative bacteria growth, with exception of P. aeruginosa. Nevertheless, no antifungal activity was observed at the tested concentrations.
authors.affiliation: CIMO/Escola Superior Agrária, Instituto Politécnico de Bragança, Campus Sta Apolónia, Apartado 1172, 5301-855 Bragança, Portugal.
authors.forename: Anabela
authors.initials: A
authors.lastname: Sousa
authors.forename: Isabel C F R
authors.initials: IC
authors.lastname: Ferreira
authors.forename: Ricardo
authors.initials: R
authors.lastname: Calhelha
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.forename: Rosa
authors.initials: R
authors.lastname: Seabra
authors.forename: Letícia
authors.initials: L
authors.lastname: Estevinho
authors.forename: Albino
authors.initials: A
authors.lastname: Bento
authors.forename: José Alberto
authors.initials: JA
authors.lastname: Pereira
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005658:Fungi / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D031658:Olea / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D011174:Portugal
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010636:Phenols; D010936:Plant Extracts