Geranylgeranyl-pyrophosphate (GGPP) synthase is down-regulated during differentiation of osteoblastic cell line MC3T3-E1.
Isoprenylation of geranylgeranyl-pyrophosphate (GGPP) is critical for activation of small GTPases. We examined the roles of GGPP synthase (GGPPS) during the differentiation induced by the cell-to-cell contact in osteoblastic cell line MC3T3-E1 cells. We found that (1) both mRNA and protein expression of GGPPS was reduced with decrement of its activity during the differentiation, (2) GGOH, which is converted to GGPP in the cells, inhibited differentiation. These results suggest that the decrement of GGPP is critical for the cell-to-cell contact-induced differentiation, in which the down-regulation of GGPPS might be involved.
Yoshida
Tomohiko
T
Department of Clinical Cell Biology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chiba-city, Chiba 260-8670, Japan.
Asanuma
Mie
M
Grossmann
Laura
L
Fuse
Masami
M
Shibata
Takahisa
T
Yonekawa
Taeko
T
Tanaka
Tomoaki
T
Ueno
Koichi
K
Yasuda
Tatsuji
T
Saito
Yasushi
Y
Tatsuno
Ichiro
I
D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D015536:Down-Regulation / Q000502:physiology*; D004789:Enzyme Activation / Q000502:physiology; D051231:Farnesyltranstransferase / Q000096:biosynthesis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D051379:Mice; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
C495428:Ggps1 protein, mouse; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D051231:Farnesyltranstransferase; D020559:Monomeric GTP-Binding Proteins