Expression of uncoupling protein 3 and GLUT4 gene in skeletal muscle of preterm newborns: possible control by AMP-activated protein kinase.
We seek to understand the mechanism for the delayed postnatal switch between glycolytic and oxidative metabolism in preterm newborns. Our previous study [Brauner et al. (Pediatr Res 53: 691-697, 2003)] suggested impaired postnatal recruitment of the gene for mitochondrial uncoupling protein 3 (UCP3) by nutritional lipids in skeletal muscle of neonates delivered before approximately 26 wk of gestation. UCP3 is linked to lipid oxidation and may be involved in the defective development of energy metabolism in skeletal muscles of very preterm newborns. In extension of our previous study, autopsy samples of musculus quadriceps femoris from 40 mostly preterm neonates and 5 fetuses were used for quantification of transcripts for UCP3, GLUT4, and their transcriptional regulator, AMP-activated protein kinase (AMPK). The new analysis confirmed the defect in the recruitment of the UCP3 gene expression by lipids in very preterm neonates. It also suggested involvement of AMPK in the control of expression of both metabolic genes, UCP3 and GLUT4, in the skeletal muscle of the newborns. Experiments on adult C57BL/6J mice confirmed the relationships between the transcripts and supported the involvement of AMPK in the control of UCP3 gene expression.
Brauner
Petr
P
Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic.
Kopecky
Pavel
P
Flachs
Pavel
P
Kuda
Ondrej
O
Vorlicek
Jaroslav
J
Planickova
Lenka
L
Vitkova
Ivana
I
Andreelli
Fabricio
F
Foretz
Marc
M
Viollet
Benoit
B
Kopecky
Jan
J
D000328:Adult; D000818:Animals; D002648:Child; D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism; D005260:Female; D005865:Gestational Age; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000502:physiology*; D011247:Pregnancy; D047928:Premature Birth; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012189:Retrospective Studies; D000071247:Uncoupling Protein 3
D051275:Glucose Transporter Type 4; D007473:Ion Channels; D024101:Mitochondrial Proteins; D021122:Protein Subunits; C495692:SLC2A4 protein, human; C000604392:UCP3 protein, human; C000604393:Ucp3 protein, mouse; D000071247:Uncoupling Protein 3; D017868:Cyclic AMP-Dependent Protein Kinases