Construction of fingerprinting and genetic diversity of mulberry cultivars in China by ISSR markers.
The ISSR fingerprintings of 24 mulberry cultivars were constructed. Totally 80 bands were produced using 17 primers selected from 20 primers. Of them, 40 bands showed polymorphism. From the bands amplified, there were three independent ways to identify the mulberry varieties, such as unique ISSR markers, unique band patterns and a combination of the band patterns provided by different primers. ISSRs were very effective in differentiating the mulberry varieties. The mean genetic similarity coefficient, the mean Nei's gene diversity (h), and the mean Shannon's Information index (I) of mulberry cultivars were 0.8731, 0.1210, and 0.1942, respectively. This suggests that the genetic diversity of mulberry cultivars was low and the genetic base was narrow. Both UPGMA cluster and PCA (Principal Coordinates Analysis) analysis showed clear genetic relationships among the 24 mulberry cultivars. The major clusters were related to known pedigree relationships.
Zhao
Wei-Guo
WG
Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
Miao
Xue-Xia
XX
Zang
Bo
B
Zhang
Lin
L
Pan
Yi-Le
YL
Huang
Yong-Ping
YP
D002681:China; D016172:DNA Fingerprinting; D017931:DNA Primers; D021901:DNA, Intergenic / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis*; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D031625:Morus / Q000235:genetics*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D017931:DNA Primers; D021901:DNA, Intergenic; D018744:DNA, Plant; D005819:Genetic Markers