Surveyor nuclease-based genotyping of SNPs.
Surveyor nuclease, a single-strand-specific endonuclease that cleaves DNA at the 3' side of a base-mismatch site in both DNA strands, can be used for genotyping of SNPs by preparing two different types of a heteroduplex: one from mutant DNA fragments and the other from a mixture of wild-type and mutant fragments. We show an example of this technique and propose that it can be used with some advantages instead of RFLP analysis.
Mitani
Noriaki
N
Central Clinical Laboratory, Nara Medical University Hospital, Shijo-Cho 840, Kashihara, Nara 634-8522, Japan.
Tanaka
Shinobu
S
Okamoto
Yasuyuki
Y
D004247:DNA / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000378:metabolism*; D005821:Genetic Techniques; D005838:Genotype; D009154:Mutation / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D004247:DNA; D004720:Endonucleases