title: Cloning of a heavy-metal-binding protein derived from activated-sludge microorganisms.
abstract: A gene of the heavy-metal-binding protein (HMBP) was newly isolated from a genetic DNA library of activated-sludge microorganisms. HMBP was produced by transformed Escherichia coli, and the copper-binding ability of HMBP was confirmed. HMBP derived from activated sludge could be available as heavy metal adsorbents in water and wastewater treatments.
authors.affiliation: Department of Civil Engineering, Graduate School of Engineering, Tohoku University, Aoba 06, Sendai 980-8579, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sano
authors.forename: Ken
authors.initials: K
authors.lastname: Myojo
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Omura
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D019216:Metals, Heavy / Q000378:metabolism*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D014170:Transformation, Genetic; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D019216:Metals, Heavy; D011994:Recombinant Proteins; D012722:Sewage; D014874:Water Pollutants, Chemical