Effect of hyperthermia on the replication of white spot syndrome virus (WSSV) in Procambarus clarkii.
The effect of hyperthermia on the development of white spot syndrome virus (WSSV) in the crayfish Procambarus clarkii was studied by competitive PCR. Crayfish were exposed to different temperatures (24 +/- 1 and 32 +/- 1 degrees C) after WSSV injection. No mortality was observed when crayfish were held at 32 +/- 1 degrees C, but mortality reached 100% when crayfish were transferred to 24 +/- 1 degrees C. Competitive PCR showed that viral levels at 32 +/- 1 degrees C remained at 10(5) copies mg(-1) tissue, while at 24 +/- 1 degrees C levels were significantly higher, rising from 10(4) to 10(10) copies mg(-1) tissue. These results suggest that hyperthermia reduces viral replication, but does not eliminate viral particles from WSSV-infected crayfish.
Du
Hua-Hua
HH
Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Feed Science Institute, Zhejiang University, Hangzhou, PR China.
Li
Wei-Fen
WF
Xu
Zi-Rong
ZR
Kil
Zong-Su
ZS
D000818:Animals; D003400:Astacoidea / Q000821:virology*; D017931:DNA Primers / Q000737:chemistry; D005880:Gills / Q000821:virology; D006358:Hot Temperature; D016133:Polymerase Chain Reaction / Q000379:methods; D016019:Survival Analysis; D013997:Time Factors; D014779:Virus Replication / Q000502:physiology*; D046848:White spot syndrome virus 1 / Q000502:physiology*
D017931:DNA Primers