Identifying microRNA regulators of cell death in Drosophila.
Animal genomes contain on the order of at least hundreds of microRNAs (miRNAs). Although most remain uncharacterized, it is already clear that miRNAs regulate many biological processes. A number of Drosophila miRNAs have been identified as likely cell death regulators, but functions for most have simply not been explored. Here we describe a protocol for identifying miRNAs that can act as cell death regulators. We also describe a simple protocol for testing roles for mRNAs identified as candidate miRNA targets using computational or other approaches.
Chen
Chun-Hong
CH
Division of Biology, California Institute of Technology, Pasadena, CA, USA.
Guo
Ming
M
Hay
Bruce A
BA
D000818:Animals; D016923:Cell Death / Q000502:physiology; D019295:Computational Biology / Q000379:methods; D004330:Drosophila / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*
D035683:MicroRNAs