Role of the nitric oxide pathway in ischemia-reperfusion injury in isolated perfused guinea pig lungs.
We examined the role of the nitric oxide (NO) pathway on ischemia-reperfusion injury via the use of isolated perfused guinea pig lungs. We administered both L-Arginine and N-nitro-L-arginine methyl ester (L-NAME) to the lungs in or after 3 h of ischemia. We observed pulmonary artery pressures as well as tissue and perfusate malondialdehyde (MDA) and glutathione (GSH) levels. We observed that L-NAME significantly increased both tissue and perfusate GSH levels and pulmonary artery pressures, but it decreased both tissue and perfusate MDA levels. On the other hand, L-arginine significantly decreased pulmonary artery pressure and both tissue and perfusate glutathione levels, but it increased both tissue and perfusate MDA levels. Electron microscopic evaluation supported our findings by indicating the preservation of lamellar bodies of type II pneumocytes. We concluded that L-NAME administration during reperfusion improves lung recovery from ischemic injury.
Altunkaya
Aykut
A
Department of Thoracic Surgery, Karaelmas University, Faculty of Medicine, Zonguldak, Turkey.
Oz
Eser
E
Sivrikoz
M Cumhur
MC
Halit
Velit
V
Yener
Nuran
N
Erdoĝan
Deniz
D
Ozoĝul
Candan
C
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006168:Guinea Pigs; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D015427:Reperfusion Injury / Q000188:drug therapy*
D004791:Enzyme Inhibitors; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester