Performance of expression vector, pTD1, in insect cell-free translation system.
We constructed a pTD1 vector for an insect cell-free translation system containing a 5' untranslated region (UTR) of a polyhedrin gene as a translational enhancer sequence. Its translational efficiency was about 50-fold higher than those of mRNAs without an enhancer sequence. Moreover, the pTD1 vector functioned as an effective expression vector not only in the insect cell-free translation system but also in wheat germ extract and rabbit reticulocyte lysate systems.
Suzuki
Takashi
T
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation, Kyoto 604-8511, Japan.
Ito
Masaaki
M
Ezure
Toru
T
Kobayashi
Shinichiro
S
Shikata
Masamitsu
M
Tanimizu
Koji
K
Nishimura
Osamu
O
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002474:Cell-Free System; D005786:Gene Expression Regulation; D024861:Genetic Enhancement / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D000077183:Occlusion Body Matrix Proteins; D014176:Protein Biosynthesis / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D018411:Spodoptera / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection / Q000379:methods; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*; D015678:Viral Structural Proteins
D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus