Crystallization and preliminary X-ray crystallographic studies of Mycobacterium tuberculosis CRP/FNR family transcription regulator.
CRP/FNR family members are transcription factors that regulate the transcription of many genes in Escherichia coli and other organisms. Mycobacterium tuberculosis H37Rv contains a probable CRP/FNR homologue encoded by the open reading frame Rv3676. The deletion of this gene is known to cause growth defects in cell culture, in bone marrow-derived macrophages and in a mouse model of tuberculosis. The mycobacterial gene Rv3676 shares approximately 32% sequence identity with prototype E. coli CRP. The structure of the protein might provide insight into transcriptional regulation in the pathogen by this protein. The M. tuberculosis CRP/FNR transcription regulator was crystallized in space group P2(1)2(1)2(1), with unit-cell parameters a = 54.1, b = 84.6, c = 101.2 A. The crystal diffracted to a resolution of 2.9 A. Matthews coefficient and self-rotation function calculations reveal the presence of two monomers in the asymmetric unit.
Akif
Mohd
M
Centre for DNA Fingerprinting and Diagnostics, ECIL Road, Nacharam, Hyderabad 500076, India.
Akhter
Yusuf
Y
Hasnain
Seyed E
SE
Mande
Shekhar C
SC
D001426:Bacterial Proteins / Q000737:chemistry* / Q000502:physiology; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D002373:Cyclic AMP Receptor Protein; D029968:Escherichia coli Proteins / Q000737:chemistry; D007506:Iron-Sulfur Proteins / Q000737:chemistry; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000472:pathogenicity; D011956:Receptors, Cell Surface / Q000737:chemistry; D014157:Transcription Factors / Q000737:chemistry* / Q000502:physiology
D001426:Bacterial Proteins; C513697:CRP protein, Mycobacterium tuberculosis; D002373:Cyclic AMP Receptor Protein; D029968:Escherichia coli Proteins; C060704:FNR protein, E coli; D007506:Iron-Sulfur Proteins; D011956:Receptors, Cell Surface; D014157:Transcription Factors; C481662:crp protein, E coli