title: Trimerisation is important for the function of clathrin at the mitotic spindle.
abstract: Clathrin is a triskelion consisting of three heavy chains each with an associated light chain. During mitosis, clathrin contributes to kinetochore fibre stability. As the N-terminal domain at the foot of each leg can bind to the mitotic spindle, we proposed previously a ;bridge hypothesis' wherein clathrin acts as a brace between two or three microtubules within a kinetochore fibre to increase fibre stability. Here, we have tested this hypothesis by replacing endogenous clathrin heavy chain in human cells with a panel of clathrin constructs. Mutants designed to abolish trimerisation were unable to rescue the mitotic defects caused by depletion of endogenous clathrin. By contrast, stunted triskelia with contracted legs could partially rescue normal mitosis. These results indicate that the key structural features of clathrin that are necessary for its function in mitosis are a trimeric molecule with a spindle interaction domain at each end, supporting the bridge hypothesis for clathrin function in mitosis.
authors.affiliation: MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK. S.J.Royle@liverpool.ac.uk
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Royle
authors.forename: Leon
authors.initials: L
authors.lastname: Lagnado
mesh_terms: D002478:Cells, Cultured; D002966:Clathrin / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D033922:Clathrin Heavy Chains / Q000378:metabolism; D033941:Clathrin Light Chains / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology; D006801:Humans; D008938:Mitosis / Q000502:physiology; D008958:Models, Molecular; D011108:Polymers / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D034622:RNA Interference / Q000502:physiology; D034741:RNA, Small Interfering / Q000009:adverse effects / Q000378:metabolism; D008941:Spindle Apparatus / Q000502:physiology*
chemical_list: D002966:Clathrin; D033941:Clathrin Light Chains; D011108:Polymers; D034741:RNA, Small Interfering; D033922:Clathrin Heavy Chains