Identification and characterization of a major Zn(II) resistance determinant of Mycobacterium smegmatis.
A zinc ion-sensitive mutant of Mycobacterium smegmatis was isolated. The transposon insertion was located in zitA (MSMEG0750), a gene coding for a cation diffusion facilitator family protein. Zinc ions specifically induced expression of zitA. In silico analysis revealed that environmental and opportunistic pathogenic species contain higher numbers of cation diffusion facilitator genes than do obligate pathogens.
Grover
Amit
A
Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi 110007, India.
Sharma
Rakesh
R
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004251:DNA Transposable Elements; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D016254:Mutagenesis, Insertional; D020102:Mycobacterium smegmatis / Q000187:drug effects* / Q000235:genetics*; D015032:Zinc / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D015032:Zinc