[A clinical study of intraocular malignant lymphoma].
OBJECTIVE
We investigated whether vitreous cytology and the measurement of intravitreous cytokine were useful for the diagnosis of intraocular malignant lymphoma.


METHODS
8 eyes of 5 patients with suspected intraocular malignant lymphoma during the past 15 months. 3 vitreous samples were collected from 3 patients at the time of pars plana vitrectomy. Polymerase chain reaction(PCR) amplification and flowcytometric analysis(FACS) of the vitreous samples were performed. Interleukin (IL)-10 and IL-6 concentrations were measured.


RESULTS
Vitreous cytology showed increased atypical B lymphocytes. The vitreous IL-10/IL-6 ratio was higher than 1 in all cases. Monoclonal rearrangement of the immunoglobulin heavy chain gene and the light chain restriction of immunoglobulin were detected.


CONCLUSIONS
The detection of the monoclonality of infiltrated cells into the vitreous by PCR amplification and FACS, and the measurements of IL-10 and IL-6 concentrations in the vitreous fluid may be useful in the diagnosis of intraocular malignant lymphoma.
Ohta
Aki
A
Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan.
Ebihara
Nobuyuki
N
Hiratsuka
Yoshimune
Y
Yokoyama
Toshiyuki
T
Murakami
Akira
A
Oshimi
Kazuo
K
D000328:Adult; D000368:Aged; D005134:Eye Neoplasms / Q000175:diagnosis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D008223:Lymphoma / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014821:Vitrectomy; D014822:Vitreous Body / Q000473:pathology
D015850:Interleukin-6; D016753:Interleukin-10