A chemical reporter strategy to probe glycoprotein fucosylation.
Fucosylated glycoproteins are involved in many cell-cell recognition events and are markers of embryonic and malignant tissue. Here we report a method for rapid profiling of fucosylated glycoproteins from human cells using 6-azido fucose as a metabolic label.
Rabuka
David
D
Department of Chemistry, University of California, Berkeley, California, USA.
Hubbard
Sarah C
SC
Laughlin
Scott T
ST
Argade
Sulabha P
SP
Bertozzi
Carolyn R
CR
D001386:Azides / Q000737:chemistry / Q000378:metabolism; D005434:Flow Cytometry; D005643:Fucose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D008958:Models, Molecular; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism
D001386:Azides; D006023:Glycoproteins; D011134:Polysaccharides; D005643:Fucose