title: [Preparation and identification of rabbit antibody against LRR of Nogo-66 receptor (NgR)].
abstract: OBJECTIVE
  To prepare rabbit antibody against LRR of Nogo-66 receptor (NgR) and identify its properties.
  
  
  METHODS
  LRR protein was expressed in E.coli BL21. Rabbits were immunized with purified LRR protein to prepare anti-LRR antibody. The titer and specificity of prepared antibodies were identified by Western blot and immunohistochemical staining, respectively.
  
  
  RESULTS
  Purified rabbit anti-rat LRR antibody with high titer was obtained. The anti-LRR Ab showed good specificity and could be used to detect the NgR in rat brain and spinal cord tissues. Immunohistochemical staining indicated that NgR was expressed widely in spinal cord neurons.
  
  
  CONCLUSIONS
  Successful preparation of anti-rat LRR antibody provides a useful tool for identification and further functional study of NgR molecule.
authors.affiliation: Department of Neurobiology, Medical School of Shanghai Jiaotong University, Shanghai 200025, China.
authors.forename: Pan-pan
authors.initials: PP
authors.lastname: Yu
authors.forename: Hui-qin
authors.initials: HQ
authors.lastname: Zhu
authors.forename: Pei-hua
authors.initials: PH
authors.lastname: Lu
authors.forename: Li-dong
authors.initials: LD
authors.lastname: Huang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000276:immunology*; D000918:Antibody Specificity; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D058851:GPI-Linked Proteins; D015870:Gene Expression; D008297:Male; D009185:Myelin Proteins / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology*; D000070816:Nogo Receptor 1; D011817:Rabbits; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology*; D020449:Repetitive Sequences, Amino Acid; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism
chemical_list: D000906:Antibodies; D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D000070816:Nogo Receptor 1; D011956:Receptors, Cell Surface; C486581:Rtn4r protein, rat