An oxygen imaging system for medical applications: preliminary results.
The examination of functional processes in tissue is gaining importance in medical research. As a result the imaging and monitoring of biochemical parameters in vivo is the goal of many imaging methods. One key parameter in photodynamic therapy (PDT) is the molecular oxygen concentration. Two-dimensional monitoring of oxygen is demanded for PDT but has not yet been achieved. The use of optical methods provides a possible means of measuring molecular oxygen. The basis of this method is the measurement of the luminescence lifetime of a dye that is quenched by molecular oxygen. The molecular oxygen concentration can be monitored two-dimensionally by pixel-wise determination of the luminescence lifetime with a CCD-camera. A new O(2)-imaging system based on this principle is presented in this article. The dye Ru(bpy)(3)(2+) is quenched by molecular oxygen and was used in the first experiments with the system.
Lochmann
Cornelia
C
Charité-Universitätsmedizin Berlin, Institut für Medizinische Physik und Lasermedizin, Fabeckstr. 60-62, 14195 Berlin, Germany. cornelia.lochmann@charite.de
Hansel
Thomas
T
Häupl
Tilmann
T
Beuthan
Jürgen
J
D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D010092:Oximetry / Q000295:instrumentation* / Q000379:methods; D010865:Pilot Projects; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods