Recombinant haemagglutinin neuraminidase antigen-based single serum dilution ELISA for rapid serological profiling of Newcastle disease virus.
A recombinant haemagglutinin neuraminidase (HN) antigen-based single serum dilution enzyme linked immuno-sorbent assay (ELISA) was developed to measure the specific antibody in sera of chickens against Newcastle disease virus. A linear relationship was found between the predicted antibody titres at a single working dilution of 1:1000 and the corresponding observed serum titres as determined by the standard serial dilution method. Regression analysis was used to determine a standard curve from which an equation was derived that allowed the demonstration of this correlation. The equation was then used to convert the corrected absorbance readings of the single working dilution directly into the predicted ELISA antibody titres. The assay proved to be sensitive, specific and accurate as compared to the standard haemagglutination inhibition (HI) test.
Mohan
C Madhan
CM
Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Bareilly 243122, Uttar Pradesh, India. sohinimadhan@yahoo.com
Dey
Sohini
S
Rai
Anant
A
Kataria
J M
JM
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015707:HN Protein / Q000235:genetics / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D009521:Newcastle Disease / Q000175:diagnosis* / Q000276:immunology; D009522:Newcastle disease virus / Q000145:classification / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D013223:Statistics as Topic
D000914:Antibodies, Viral; D015707:HN Protein; D011994:Recombinant Proteins