Application of high throughput cell array technology to FISH: investigation of the role of deletion of p16 gene in leukemias.
Bio-cell chip is a chip that has hundreds of types of cells arrayed and immobilized on a small slide. To elucidate the role of deletion of the p16 gene in hematologic malignancies, the bio-cell chip technique was applied to fluorescent in situ hybridization (FISH) study. We made a bio-cell chip with bone marrow specimen from 109 patients with acute lymphoblastic leukemia (ALL), 102 patients with acute myelogenous leukemia (AML), 47 patients with chronic myelogenous leukemia (CML), and 25 patients with multiple myeloma (MM). A glass slide with 96 separated areas was fabricated, onto which was added methanol/acetic acid fixed cell suspensions for high-throughput FISH for p16. With the successful application of bio-cell chip technique, we found that the deletion of p16 contributed to the oncogenesis in acute leukemia, but not in chronic leukemia. In conclusion, the bio-cell chip, a cell version of ultrahigh-throughput technology, was successfully applied to the FISH study, which can be utilized efficiently in the molecular cytogenetic investigation of hematologic malignancies.
Lee
Dong Soon
DS
Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
Lee
Jee Hyung
JH
Min
Hyun Chung
HC
Kim
Tae Young
TY
Oh
Bo Ra
BR
Kim
Ho Young
HY
Lee
Jong Yun
JY
Lee
Chung Kee
CK
Chun
Hong Gu
HG
Kim
Hee Chan
HC
D000208:Acute Disease; D002908:Chronic Disease; D020732:Cytogenetic Analysis / Q000379:methods; D005260:Female; D017353:Gene Deletion; D019942:Genes, p16; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007938:Leukemia / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D046228:Microarray Analysis; D009101:Multiple Myeloma / Q000175:diagnosis / Q000235:genetics*; D011237:Predictive Value of Tests