YAC/BAC contig spanning the MHC class III region of cattle.
A contig of the class III region of the bovine major histocompatibility complex (MHC) was established from bacterial and yeast artificial chromosomes using PCR and BAC-end sequencing. The marker content of individual clones was determined by gene and BAC-end specific PCR, and the location of genes and BAC-ends was confirmed analyzing somatic hybrid cells. A comparative analysis indicated that the content and order of MHC class III genes is strongly conserved between cattle and other mammalian species. Fluorescence in situ hybridization localized the bovine class III region to BTA23q21-->q22. The results show that the collection of sequenced BAC-ends is a powerful resource for generating high-resolution comparative chromosome maps.
Gelhaus
A
A
Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Hess
M
M
Förster
B
B
Goldammer
T
T
Schwerin
M
M
Horstmann
R D
RD
D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D018244:Chromosomes, Artificial, Yeast; D020451:Contig Mapping; D006649:Histocompatibility Antigens / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008285:Major Histocompatibility Complex / Q000235:genetics*; D016133:Polymerase Chain Reaction
D006649:Histocompatibility Antigens