title: Dependence of lung injury on surface tension during low-volume ventilation in normal open-chest rabbits.
abstract: To evaluate the role of pulmonary surfactant in the prevention of lung injury caused by mechanical ventilation (MV) at low end-expiratory volumes, lung mechanics and morphometry were assessed in three groups of eight normal, open-chest rabbits ventilated for 3-4 h at zero end-expiratory pressure (ZEEP) with physiological tidal volumes (Vt = 10 ml/kg). One group was left untreated (group A); the other two received surfactant intratracheally (group B) or aerosolized dioctylsodiumsulfosuccinate (group C) before MV on ZEEP. Relative to initial MV on positive end-expiratory pressure (PEEP; 2.3 cmH(2)O), quasi-static elastance (Est) and airway (Rint) and viscoelastic resistance (Rvisc) increased on ZEEP in all groups. After restoration of PEEP, only Rint (124%) remained elevated in group A, only Est (36%) was significantly increased in group B, whereas in group C, Est, Rint, and Rvisc were all markedly augmented (274, 253, and 343%). In contrast, prolonged MV on PEEP had no effect on lung mechanics of eight open-chest rabbits (group D). Lung edema developed in group C (wet-to-dry ratio = 7.1), but not in the other groups. Relative to group D, both groups A and C, but not B, showed histological indexes of bronchiolar injury, whereas all groups exhibited an increased number of polymorphonuclear leukocytes in alveolar septa, which was significantly greater in group C. In conclusion, administration of exogenous surfactant largely prevents the histological and functional damage of prolonged MV at low lung volumes, whereas surfactant dysfunction worsens the functional alterations, also because of edema formation and, possibly, increased inflammatory response.
authors.affiliation: Istituto di Fisiologia Umana I, Università di Milano, Milan, Italy. edgardo.dangelo@unimi.it
authors.forename: Edgardo
authors.initials: E
authors.lastname: D'Angelo
authors.forename: Matteo
authors.initials: M
authors.lastname: Pecchiari
authors.forename: Guendalina
authors.initials: G
authors.lastname: Gentile
mesh_terms: D000818:Animals; D004143:Dioctyl Sulfosuccinic Acid / Q000494:pharmacology; D004548:Elasticity; D008168:Lung / Q000473:pathology / Q000503:physiopathology*; D055370:Lung Injury; D008176:Lung Volume Measurements; D008433:Mathematics; D011175:Positive-Pressure Respiration; D011817:Rabbits; D012121:Respiration, Artificial / Q000009:adverse effects*; D015656:Respiratory Mechanics / Q000187:drug effects / Q000502:physiology*; D013500:Surface Tension / Q000187:drug effects; D013501:Surface-Active Agents / Q000494:pharmacology; D013990:Tidal Volume / Q000187:drug effects / Q000502:physiology
chemical_list: D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid