A modified process for preparing cationic polylactide-co-glycolide microparticles with adsorbed DNA.
We have previously shown that cationic polylactide-co-glycolide (PLG) microparticles can be effectively used to adsorb DNA and generate potent immune responses in vivo. We now describe a modified and easier process containing a single lyophilization step to prepare these cationic PLG microparticles with adsorbed DNA. Cationic PLG microparticle formulations with adsorbed DNA were prepared using a modified solvent evaporation technique. Formulations with a fixed CTAB content and DNA load were prepared. The loading efficiency and 24h DNA release was evaluated for each formulation and compared to the earlier method of preparation. Select formulations were tested in vivo. The modified cationic PLG microparticle preparation method with a single lyophilization step, showed comparable physico-chemical behaviour to the two lyophilization steps process and induced comparable immune. The modified process with a single lyophilization step is a more practical process and can be utlized to prepare cationic PLG microparticles with adsorbed DNA on a large scale.
Singh
Manmohan
M
Novartis Vaccines, 4560 Horton Street, Emeryville, CA 94608, USA. manmohan_singh@chiron.com
Fang
Jia-Hwa
JH
Kazzaz
Jina
J
Ugozzoli
Mildred
M
Chesko
James
J
Malyala
Padma
P
Dhaliwal
Raj
R
Wei
Rebecca
R
Hora
Maninder
M
O'Hagan
Derek
D
D000327:Adsorption; D000818:Animals; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000008:administration & dosage / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry*; D004339:Drug Compounding; D005260:Female; D005612:Freeze Drying; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D011098:Polyglactin 910 / Q000008:administration & dosage / Q000737:chemistry*
D002593:Cetrimonium Compounds; D007074:Immunoglobulin G; D011098:Polyglactin 910; D004247:DNA; D000077286:Cetrimonium