title: [Effects of Guben Hutan Tongmai Recipe on expressions of macrophages and cell adhesion molecules of aortic endothelia in rats with syndrome of phlegm blocking blood vessel].
abstract: OBJECTIVE
  To observe the effects of Guben Huatan Tongmai Recipe (GBHTTMR), a compound Chinese herbal recipe, on expressions of macrophages and cell adhesion molecules (CAMs) of aortic endothelia in rats with syndrome of phlegm blocking blood vessel, and to explore the pathogenesis of the phlegm-pathogen.
  
  
  METHODS
  Fifty normal male Wistar rats, 7-week in age, were randomly divided into five groups: normal control group, untreated group, high-dose GBHTTMR-treated group, low-dose GBHTTMR-treated group and simvastatin-treated group, with 10 rats in each group. Syndrome of phlegm blocking blood vessel was induced in rats of the latter 4 groups by feeding the rats with high lipid diet. Levels of blood lipid were compared among the 5 groups. The expressions of macrophages and CAMs in aortic endothelia were tested by immunohistochemical staining method.
  
  
  RESULTS
  The level of blood lipid, and the expressions of macrophages and CAMs showed statistical differences between the normal control group and the untreated group (P<0.01), and between the untreated group and the low-, high-dose GBHTTMR-treated and simvastatin-treated groups as well (P<0.05).
  
  
  CONCLUSIONS
  GBHTTMR can decrease the level of serum cholesterol and triglycerides, and increase the level of high density lipoprotein. It also can inhibit the expressions of macrophages, intercellular adhesion molecule-1, vascular cell adhesion molecule-1, P-selectin, and E-selectin.
authors.affiliation: Institute of Geratology, Hubei College of Traditional Chinese Medicine, Wuhan, Hubei Province 430061, China. pwang54@yahoo.com.cn
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Guo
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Liu
authors.forename: Dai-Zhi
authors.initials: DZ
authors.lastname: Tian
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Zou
authors.forename: Fang-Ping
authors.initials: FP
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002784:Cholesterol / Q000097:blood; D003937:Diagnosis, Differential; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D006937:Hypercholesterolemia / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D000960:Hypolipidemic Agents / Q000494:pharmacology; D007150:Immunohistochemistry; D008055:Lipids / Q000008:administration & dosage / Q000097:blood / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008516:Medicine, Chinese Traditional; D008517:Phytotherapy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D019821:Simvastatin / Q000494:pharmacology; D013577:Syndrome; D014280:Triglycerides / Q000097:blood
chemical_list: D015815:Cell Adhesion Molecules; D004365:Drugs, Chinese Herbal; D000960:Hypolipidemic Agents; D008055:Lipids; D014280:Triglycerides; C527181:guben hutan tongmai; D002784:Cholesterol; D019821:Simvastatin