title: Plasmid-mediated quinolone resistance determinant qnrS in Enterobacter cloacae.
abstract: PCR was used to investigate the occurrence of the plasmid-encoded quinolone resistance determinants qnrA and qnrS among diarrhoeagenic enterobacterial isolates recovered from Hanoi, Vietnam, during the period March 2001 to April 2002. In total, 162 Escherichia coli isolates, 28 Shigella isolates and three Enterobacter cloacae isolates were negative for qnrA, while a single Ent. cloacae isolate harboured a 50-kb qnrS-positive conjugative plasmid. Cloning and sequencing identified a qnrS gene bracketed by open reading frames identical to those surrounding the qnrS gene of a Shigella flexneri isolate from Japan, thereby suggesting a common mechanism of acquisition.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Faculté de Médecine Paris-Sud, Université Paris XI, Le Kremlin-Bicêtre, France.
authors.forename: L
authors.initials: L
authors.lastname: Poirel
authors.forename: T Vu
authors.initials: TV
authors.lastname: Nguyen
authors.forename: A
authors.initials: A
authors.lastname: Weintraub
authors.forename: C
authors.initials: C
authors.lastname: Leviandier
authors.forename: P
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D016972:Enterobacter cloacae / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000378:metabolism*; D015363:Quinolones / Q000494:pharmacology*; D014744:Vietnam / Q000453:epidemiology
chemical_list: D001426:Bacterial Proteins; D015363:Quinolones