[Immune response induced by combinant immunization of different HBsAg vaccines in mice].
OBJECTIVE
To investigate the specific humoral and cellular immune response induced by prime-boost immunization of HBsAg protein vaccine (P), recombinant vaccinia virus vaccine (V) and DNA vaccine (D) in mice.


METHODS
Groups of BALB/c mice were primed by one of the three vaccines P, V or D and boosted by another vaccine at 2, 5, 8 and 11 week later, thus 9 immune combinations were made: PP, PV, PD, VP, VV, VD, DP, DV and DD. Serum samples were collected at week 2, 5, 8 and 11 and levels of anti-HBsAg IgG antibodies and their sub-isotypes were determined. Seven days after every boost, spleen cells of vaccinated mice were separated and the specific CTL lysis ratio of P815S cells were determined.


RESULTS
Among the three HBsAg vaccines P, V and D, V could induce the quickest humoral immune response. The memory humoral immune response induced by P was the strongest. D induced the weakest antibody titer. The ratio of specific IgG1/IgG2a indicated that antibody induced by PP was more polarized to Th2. The other groups induced balanced Th1/Th2 immune response. Among all the groups, VD and DV induced the strongest CTL response, and the specific lysis ratio of P815S cells was 71% and 64%, respectively.


CONCLUSIONS
The experimental results suggested that among all the immune combinations, PV, PD, VP and VD can induce better humoral immune response while DV and VD can induce stronger CTL response in BALB/c mice.
Wu
Ting
T
Research Center for Medical Molecular Virology of Fujian Province, Xiamen University, Xiamen 361005, China. wuting@xmu.edu.cn
Chen
Min
M
Ou
Shan-hai
SH
Yan
Yuan-qing
YQ
Wu
Xiao-lu
XL
Zhang
Jun
J
Xia
Ning-shao
NS
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D002460:Cell Line; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D007114:Immunization / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology; D050440:Immunoglobulin Subunits / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D017778:Vaccines, Combined / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D006514:Hepatitis B Surface Antigens; D007074:Immunoglobulin G; D050440:Immunoglobulin Subunits; D017778:Vaccines, Combined; D019444:Vaccines, DNA; D014765:Viral Vaccines