[Differential expression of immune-associated genes in two subcloned cell lines from a same human bladder cancer].
OBJECTIVE
To screen and identify differentially expressed genes in subcloned lines from a same human bladder transitional cell carcinoma (TCC).


METHODS
Two bladder TCC cell lines (BLX and BLS-211) with different phenotypes but same origin were used to screen for differentially expressed genes by suppression subtractive hybridization (SSH).


RESULTS
9 over-expressed genes in BLX and 15 in BLS-211 cells were obtained, respectively. Some Bacillus Galmette-Guerin(BCG)- associated genes, such as BCG induced integral membrane protein(BIGM103), fibronectin(FN), complement factor B(BF), were over-expressed in BLX cells. And 8 new ESTs(Expressed Sequence Tag) in BLS-211 cells were collected by GenBank dbEST database with the accession number of DY505708-13, DY230447-8.


CONCLUSIONS
SSH is a powerful method for the identification of differentially expressed genes in different cell lines or clones. Some BCG-associated genes, which are differentially expressed in different cells may contribute to the different response to clinical BCG therapy. The identified new ESTs can be cloned for full length to further study their functions.
Yang
Yu-cong
YC
Medical Experimental Centre, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. yangyucong@eyou.com
Li
Xu
X
Chen
Wei
W
D002295:Carcinoma, Transitional Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D045744:Cell Line, Tumor; D002999:Clone Cells / Q000378:metabolism; D019295:Computational Biology; D004587:Electrophoresis, Agar Gel; D020224:Expressed Sequence Tags; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*