Ribosomal DNA intergenic spacer sequence in foxtail millet, Setaria italica (L.) P. Beauv. and its characterization and application to typing of foxtail millet landraces.
We determined the sequence of ribosomal DNA (rDNA) intergenic spacer (IGS) of foxtail millet isolated in our previous study, and identified subrepeats in the polymorphic region. We also developed a PCR-based method for identifying rDNA types based on sequence information and assessed 153 accessions of foxtail millet. Results were congruent with our previous works. This study provides new findings regarding the geographical distribution of rDNA variants. This new method facilitates analyses of numerous foxtail millet accessions. It is helpful for typing of foxtail millet germplasms and elucidating the evolution of this millet.
Fukunaga
Kenji
K
Laboratory of Crop Evolution, Faculty of Agriculture, Kyoto University, Mukoh, Japan. kfukunag@nichibun.ac.jp
Ichitani
Katsuyuki
K
Taura
Satoru
S
Sato
Muneharu
M
Kawase
Makoto
M
D001483:Base Sequence; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods; D032983:Setaria Plant / Q000145:classification / Q000235:genetics*; D013045:Species Specificity
D021901:DNA, Intergenic; D018744:DNA, Plant; D004275:DNA, Ribosomal