title: Erythrovirus B19 infection in acquired immunodeficiency syndrome: screening by histopathology, immunohistochemistry, and in situ hybridization.
abstract: Erythrovirus B19 infects erythrocytic progenitors, transiently interrupting erythropoiesis. In AIDS patients it causes chronic anemia amenable to treatment. We looked for evidences of B19 infection in stored bone marrow material from patients with acquired immunodeficiency syndrome. Histological sections were made from stored paraffin blocks from 33 autopsies (39 blocks) and 35 biopsies (45 blocks, 30 patients) performed from 1988 to 2002. They were examined after hematoxylin-eosin (HE) staining, immunohistochemical (IHC), and in situ hybridization. HE revealed intra-nuclear inclusion bodies ("lantern cells") suggesting B19 infection in 19 sections corresponding to 19 of 63 patients examined with this test. Seven of 78 sections subjected to immunohistochemistry were positive, corresponding to 7 of 58 patients examined with this test. Fourteen sections corresponding to 13 of the 20 HE and/or IHC positive patients were subjected to in situ hybridization, with six positives results. Among the 13 patients subjected to the three techniques, only one gave unequivocal positive results in all and was considered a true positive. The frequency of B19 infection (1/63 patients) in the material examined can be deemed low.
authors.affiliation: Departamento de Patologia, Universidade Federal Fluminense, Niteroi, RJ, Brasil.
authors.forename: Sérgio
authors.initials: S
authors.lastname: Setúbal
authors.forename: Solange Artimos
authors.initials: SA
authors.lastname: de Oliveira
authors.forename: Andréia Rodrigues Cordovil
authors.initials: AR
authors.lastname: Pires
authors.forename: Eliene Carvalho
authors.initials: EC
authors.lastname: da Fonseca
authors.forename: Luiz Antônio Bastos
authors.initials: LA
authors.lastname: Camacho
authors.forename: Ana Cristina Freire
authors.initials: AC
authors.lastname: Seródio
authors.forename: Jussara Pereira
authors.initials: JP
authors.lastname: do Nascimento
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology / Q000821:virology*; D001856:Bone Marrow Examination / Q000379:methods; D004801:Eosine Yellowish-(YS); D005260:Female; D006416:Hematoxylin; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D016612:Paraffin Embedding; D010322:Parvoviridae Infections / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D016732:Parvovirus B19, Human / Q000302:isolation & purification*
chemical_list: D004801:Eosine Yellowish-(YS); D006416:Hematoxylin