Role of N-acetylglucosamine within core lipopolysaccharide of several species of gram-negative bacteria in targeting the DC-SIGN (CD209).
Our recent studies have shown that the dendritic cell-specific ICAM nonintegrin CD209 (DC-SIGN) specifically binds to the core LPS of Escherichia coli K12 (E. coli), promoting bacterial adherence and phagocytosis. In this current study, we attempted to map the sites within the core LPS that are directly involved in LPS-DC-SIGN interaction. We took advantage of four sets of well-defined core LPS mutants, which are derived from E. coli, Salmonella enterica serovar Typhimurium, Neisseria gonorrhoeae, and Haemophilus ducreyi and determined interaction of each of these four sets with DC-SIGN. Our results demonstrated that N-acetylglucosamine (GlcNAc) sugar residues within the core LPS in these bacteria play an essential role in targeting the DC-SIGN receptor. Our results also imply that DC-SIGN is an innate immune receptor and the interaction of bacterial core LPS and DC-SIGN may represent a primeval interaction between Gram-negative bacteria and host phagocytic cells.
Zhang
Pei
P
Department of Biomedical Sciences, College of Medicine, University of Illinois at Chicago, Rockford, IL 61107, USA.
Snyder
Scott
S
Feng
Peter
P
Azadi
Parastoo
P
Zhang
Shusheng
S
Bulgheresi
Silvia
S
Sanderson
Kenneth E
KE
He
Johnny
J
Klena
John
J
Chen
Tie
T
D000117:Acetylglucosamine / Q000502:physiology*; D001665:Binding Sites / Q000276:immunology; D002240:Carbohydrate Sequence; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004926:Escherichia coli; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006191:Haemophilus ducreyi; D006193:Haemophilus influenzae; D006367:HeLa Cells; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012486:Salmonella typhimurium; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D000117:Acetylglucosamine; D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin