title: Succinic acid production from Bacteroides fragilis: process optimization and scale up in a bioreactor.
abstract: We report the effect of different physiological and nutritional parameters on succinic acid production from Bacteroides fragilis. This strain initially produced 0.70gL(-1) of succinic acid in 60h. However, when process optimization was employed, 5.4gL(-1) of succinic acid was produced in medium consisting of glucose (1.5%); tryptone (2.5%); Na(2)CO(3) (1.5%), at pH 7.0, when inoculated with 4% inoculum and incubated at 37 degrees C, 100rpm for 48h. A marked enhancement in succinic acid production was observed when the optimized conditions were employed in a 10L bioreactor. A total of 12.5gL(-1) of succinic acid was produced in 30h. This is approximately 12-fold increase in succinic acid production when compared to the initial un-optimized medium production. This enhancement in succinic acid production may be due to the control of CO(2) supply and the impeller speed. This is also resulted in the reduction of the production time. The present study provides useful information to the industrialists seeking environmentally benign technology for the production of bulk biomolecules through manipulation of various chemical parameters.
authors.affiliation: Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Isar
authors.forename: Lata
authors.initials: L
authors.lastname: Agarwal
authors.forename: Saurabh
authors.initials: S
authors.lastname: Saran
authors.forename: Rajendra Kumar
authors.initials: RK
authors.lastname: Saxena
mesh_terms: D000693:Anaerobiosis / Q000502:physiology; D001441:Bacteroides fragilis / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation / Q000502:physiology; D007218:Industrial Microbiology / Q000379:methods*; D019802:Succinic Acid / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D003470:Culture Media; D019802:Succinic Acid