title: Identification of cell lines permissive for human coronavirus NL63.
abstract: Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-B4, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was observed in human lung fibroblasts MRC-5s. In Rhabdomyosarcoma cells, Madin-Darby-Canine-kidney cells and in an undefined monkey kidney cell line some replication was observed but massive exponential rise in virus yield lacked The results will lead to an improved routine diagnostic algorithm for the detection of the human coronavirus NL63.
authors.affiliation: University of Bonn, Institute for Medical Microbiology, Immunology, and Parasitology, Department of Virology, and Children's Hospital, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany. schildgen@mibi03.meb.uni-bonn.de
authors.forename: Oliver
authors.initials: O
authors.lastname: Schildgen
authors.forename: Maarten F
authors.initials: MF
authors.lastname: Jebbink
authors.forename: Michel
authors.initials: M
authors.lastname: de Vries
authors.forename: Krzysztov
authors.initials: K
authors.lastname: Pyrc
authors.forename: Ronald
authors.initials: R
authors.lastname: Dijkman
authors.forename: Arne
authors.initials: A
authors.lastname: Simon
authors.forename: Andreas
authors.initials: A
authors.lastname: Müller
authors.forename: Bernd
authors.initials: B
authors.lastname: Kupfer
authors.forename: Lia
authors.initials: L
authors.lastname: van der Hoek
mesh_terms: D000818:Animals; D002460:Cell Line; D017934:Coronavirus / Q000254:growth & development*; D003588:Cytopathogenic Effect, Viral; D004285:Dogs; D000882:Haplorhini; D006801:Humans; D014776:Virus Cultivation; D014779:Virus Replication