title: Crystallization and preliminary X-ray diffraction analysis of protein 14 from Sulfolobus islandicus filamentous virus (SIFV).
abstract: A large-scale programme has been embarked upon aiming towards the structural determination of conserved proteins from viruses infecting hyperthermophilic archaea. Here, the crystallization of protein 14 from the archaeal virus SIFV is reported. This protein, which contains 111 residues (MW 13 465 Da), was cloned and expressed in Escherichia coli with an N-terminal His(6) tag and purified to homogeneity. The tag was subsequently cleaved and the protein was crystallized using PEG 1000 or PEG 4000 as a precipitant. Large crystals were obtained of the native and the selenomethionine-labelled protein using sitting drops of 100-300 nl. Crystals belong to space group P6(2)22 or P6(4)22, with unit-cell parameters a = b = 68.1, c = 132.4 A. Diffraction data were collected to a maximum acceptable resolution of 2.95 and 3.20 A for the SeMet-labelled and native protein, respectively.
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques, CNRS and Universités d'Aix-Marseille I et II, UMR 6098, Case 932, 163 Avenue de Luminy, 13288 Marseille CEDEX 9, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Goulet
authors.forename: Silvia
authors.initials: S
authors.lastname: Spinelli
authors.forename: Valérie
authors.initials: V
authors.lastname: Campanacci
authors.forename: Sophie
authors.initials: S
authors.lastname: Porciero
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Blangy
authors.forename: Roger A
authors.initials: RA
authors.lastname: Garrett
authors.forename: Herman
authors.initials: H
authors.lastname: van Tilbeurgh
authors.forename: Nicolas
authors.initials: N
authors.lastname: Leulliot
authors.forename: Tamara
authors.initials: T
authors.lastname: Basta
authors.forename: David
authors.initials: D
authors.lastname: Prangishvili
authors.forename: Christian
authors.initials: C
authors.lastname: Cambillau
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D023643:Lipothrixviridae / Q000737:chemistry*; D016944:Sulfolobus / Q000235:genetics / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D014764:Viral Proteins