title: [The influence of laser irradiation of low-power density on an experimental cartilage damage in rabbit knee-joints: an in vivo investigation considering macroscopic, histological and immunohistochemical changes].
abstract: In a total of 45 rabbits, knee-joint arthrosis was induced according to the Hulth & Telhag model. Depending on the post-operative survival time, the cartilage was investigated macroscopically, histologically and immunohistochemically (within a period of 10 days to 8 months). Thereafter, the influence of laser irradiation at a wavelength of 692.6 nm and energy densities of 1 and 4 J/cm2 on the cartilage morphology seven days following the exposure was examined. After joint instability surgery it was found out that the cartilage changes in the main stress area (MSA) and in regions outside the main stress area (ROMSA) progressed differently. Various qualitative and semi-quantitative changes were found for collagens I, II, IV and V, and for the glycoproteins fibronectin and tenascin. Immunohistochemically, there was a growing expression of collagen I in the apical layers, collagen II showed a stronger pericellular expression, and collagen IV showed, after an initial growth of the pericellular expression, a reduced territorial expression and a stronger apical-interterritorial expression in the osteoarthrotic cartilage. For fibronectin, the cellular expression turned out to grow in the ROMSA. In the MSA it decreased, but at the same time the interterritorial expression grew. For Tanascin, there was a decrease of the interterritorial expression in the radial zone while the pericellular and interterritorial expression of the apical layers of the osteoarthrotic cartilage grew. Lasing proved to significantly influence the osteoarthrotically changed cartilage when applied at an energy density of 1 J/cm2, i.e., the morphological changes had not yet progressed to the extent the control group had. Both the chondrocyte density and the glucosaminoglycan content turned out to be higher. When lasing was applied at higher energy densities, no significant difference among the control groups was found. Thus, it could be demonstrated in vivo that an arthrotic process decelerates through the influence of laser light of low-energy densities.
authors.affiliation: Orthopädische Klinik, Friederich-Alexander-Universität Erlangen-Nürnberg, Rathsberger Strasse 57, 91054 Erlangen, Deutschland.
authors.forename: David
authors.initials: D
authors.lastname: Pfander
authors.forename: Björn
authors.initials: B
authors.lastname: Jörgensen
authors.forename: Ewa
authors.initials: E
authors.lastname: Rohde
authors.forename: Uwe
authors.initials: U
authors.lastname: Bindig
authors.forename: Gerhard
authors.initials: G
authors.lastname: Müller
authors.forename: Eike
authors.initials: E
authors.lastname: Eric Scheller
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000276:immunology / Q000473:pathology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D007593:Joint Instability / Q000175:diagnosis / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D028022:Low-Level Light Therapy / Q000009:adverse effects / Q000379:methods*; D020370:Osteoarthritis, Knee / Q000150:complications / Q000276:immunology / Q000473:pathology* / Q000532:radiotherapy*; D011817:Rabbits; D011879:Radiotherapy Dosage; D016896:Treatment Outcome