title: Multiple myeloma associated with sialyl salivary-type amylase.
abstract: BACKGROUND
  There have been several reports describing a notable hyperamylasaemia in patients with multiple myeloma. Such amylase-producing myelomas have been mainly described in the context of concomitant salivary-type hyperamylasaemia, with sialyl salivary-type amylase identified in a portion of those cases. We investigated the incidence of the production of sialyl salivary-type amylase in serum of multiple myeloma patients.
  
  
  METHODS
  Eleven patients (6 male and 5 female) who had been diagnosed as having multiple myeloma were enrolled in this study. Sialyl salivary-type amylase was detected by isoamylase electrophoresis and HPLC analysis, and identified by detecting either abnormal neuraminidase-sensitive band through isoamylase electrophoresis or abnormal extra-elution peak of amylase by means of HPLC analysis.
  
  
  RESULTS
  Sialyl salivary-type amylase was detected in 7 out of 11 (63.6%) patients. Median total amylase activity was 154 U/l (range 109-43020). Isoamylase electrophoretic patterns of patients' serum were normal in 5 patients (71.4%) out of 7 patients and salivary-dominant in 2 (50.0%) out of 4 patients.
  
  
  CONCLUSIONS
  We consider that there is no significant relationship between total serum amylase level and amylase isoenzyme pattern in the incidence of production of sialyl salivary-type amylase with multiple myeloma.
authors.affiliation: Clinical Laboratory, Hokkaido University Hospital, N-14 W-5, Kitaku, Sapporo, 060-8648, Japan. mscoccus@med.hokudai.ac.jp
authors.forename: Masahiko
authors.initials: M
authors.lastname: Shigemura
authors.forename: Takanori
authors.initials: T
authors.lastname: Moriyama
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shibuya
authors.forename: Masato
authors.initials: M
authors.lastname: Obara
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Endo
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hashino
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yokouchi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Asaka
authors.forename: Chikara
authors.initials: C
authors.lastname: Shimizu
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Chiba
authors.forename: Masaharu
authors.initials: M
authors.lastname: Nishimura
mesh_terms: D000368:Aged; D000681:Amylases / Q000097:blood*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood* / Q000201:enzymology*; D012469:Salivary Glands / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D000681:Amylases