title: Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum.
abstract: Corynebacterium glutamicum forms inorganic polyphosphate (poly P) that may occur as soluble (cytosolic) poly P and/or as volutin granules. A suitable method for monitoring soluble and granular poly P in C. glutamicum was developed and applied to C. glutamicum cells cultivated under different growth conditions. Under phosphate-limiting conditions, C. glutamicum did not accumulate poly P, but it rebuilt its poly P storages when phosphate became available. The poly P content of C. glutamicum growing on glucose minimal medium with sufficient phosphate varied considerably during growth. While the poly P content was minimal in the midexponential growth phase, two maxima were observed in the early exponential growth phase and at entry into the stationary growth phase. Cells in the early exponential growth phase primarily contained granular poly P, while cells entering the stationary growth phase contained soluble, cytosolic poly P. These results and those obtained for C. glutamicum cells cultivated under hypo- or hyperosmotic conditions or during glutamate production revealed that the poly P content of C. glutamicum and the partitioning between cytosolic and granular forms of poly P are dynamics and depend on the growth conditions.
authors.affiliation: Institute of Agrosphere (ICG-IV), Research Center Juelich, Juelich, Germany. p.klauth@fz-juelich.de
authors.forename: Peter
authors.initials: P
authors.lastname: Klauth
authors.forename: Srinivas Reddy
authors.initials: SR
authors.lastname: Pallerla
authors.forename: Dolores
authors.initials: D
authors.lastname: Vidaurre
authors.forename: Carla
authors.initials: C
authors.lastname: Ralfs
authors.forename: Volker F
authors.initials: VF
authors.lastname: Wendisch
authors.forename: Siegfried M
authors.initials: SM
authors.lastname: Schoberth
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D048230:Corynebacterium glutamicum / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D004977:Ethambutol / Q000494:pharmacology; D009997:Osmotic Pressure; D011122:Polyphosphates / Q000032:analysis* / Q000378:metabolism; D011136:Polysorbates; D012995:Solubility
chemical_list: D000900:Anti-Bacterial Agents; D011122:Polyphosphates; D011136:Polysorbates; D004977:Ethambutol; C068430:polysorbate 40