[Expression of mineral-associated factors in the mineral stage tooth germ of mice].
OBJECTIVE
To study the distribution and expression of core binding factor alpha 1 (Cbf alpha1), bone sialoprotein (BSP), osteopontin (OPN), osteocalcin (OC) and alkaline phosphatase (ALP) in mineral stage tooth germ of mice, so as to understand their roles in the development of the mineral stage tooth germ.


METHODS
Thirty BALB/c mice in different days were killed, and their bilateral mandibular first molar germs with surrounding alveolar bone were taken out, then the tissues were fixed with 4% paraformaldehyde at 4 degrees C overnight, dehydrated, embedded in paraffin and serially sectioned at 5 microm. Immunohistochemical assay was adopted to determine the tissue distribution and cellular localization of Cbf alpha1, OPN, BSP, ALP and OC in the mineral stage tooth germ by these sections of BALB/c mice.


RESULTS
There were Cbf alpha1, BSP and OPN expressing in the alveolar bone, and Cbf alpha1, ALP, OC in predentin, while in other tissues such as dental follicle, pulp cells, stratum intermedium, stellate reticulum, only ALP and OC were detected, but none of them were observed in the dentin.


CONCLUSIONS
The formation mechanisms of hard tooth tissues are different. They are controlled by different factors. Cbf alpha1, ALP and OC are involved in the early formation of dentin, while Cbf alpha1, OPN and BSP involved in the early formation of alveolar bone, ALP involved in the stratum intermedium. They all play an important role in the start of mineralization.
Pan
Ke-qing
KQ
Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, Shandong Province, China.
Yang
Pi-shan
PS
Li
Shu
S
D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis*; D003804:Dentin; D058574:Integrin-Binding Sialoprotein / Q000032:analysis*; D051379:Mice; D008903:Minerals / Q000737:chemistry; D008963:Molar; D015675:Osteocalcin / Q000032:analysis*; D053495:Osteopontin / Q000032:analysis*; D014083:Tooth Germ / Q000737:chemistry* / Q000254:growth & development*
D050659:Core Binding Factor Alpha 1 Subunit; D058574:Integrin-Binding Sialoprotein; D008903:Minerals; C493716:Runx2 protein, mouse; D015675:Osteocalcin; D053495:Osteopontin; D000469:Alkaline Phosphatase