Molecular-beacon multiplex real-time PCR assay for detection of Vibrio cholerae.
A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity and sensitivity of this assay, when tested with pure culture and spiked environmental water samples, were high, surpassing those of currently published PCR assays for the detection of this organism.
Gubala
Aneta J
AJ
Human Protection and Performance Division, Defence Science and Technology Organisation, Melbourne, Australia. aneta.gubala@dsto.defence.gov.au
Proll
David F
DF
D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005809:Genes, Regulator; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics; D014871:Water Microbiology
D015342:DNA Probes; D004269:DNA, Bacterial