title: How to assess and study cell death in Dictyostelium discoideum.
abstract: In this chapter, we describe how to conveniently demonstrate, assess, and study cell death in Dictyostelium through simple cell culture, clonogenic tests, and photonic (with the help of staining techniques) and electronic microscopy. Cell death can be convniently generated using minor modifications of the monolayer technique of Rob Kay et al., and either wild-type HMX44A Dictyostelium cells or the corresponding atg1- autophagy gene mutant cells. Methods to follow cell death qualitatively and quantitatively facilitate detailed studies of vacuolar death in wild-type cells and of nonvacuolar, "condensed" death in atg1- mutant cells.
authors.affiliation: Centre d'Immunologie INSERM-CNRS-Univ. Medit. de Marseille-Luminy, France.
authors.forename: Artemis
authors.initials: A
authors.lastname: Kosta
authors.forename: Catherine
authors.initials: C
authors.lastname: Laporte
authors.forename: David
authors.initials: D
authors.lastname: Lam
authors.forename: Emilie
authors.initials: E
authors.lastname: Tresse
authors.forename: Marie-Françoise
authors.initials: MF
authors.lastname: Luciani
authors.forename: Pierre
authors.initials: P
authors.lastname: Golstein
mesh_terms: D000818:Animals; D001343:Autophagy; D016923:Cell Death; D003114:Colony-Forming Units Assay; D004023:Dictyostelium / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D005434:Flow Cytometry