Batch foam separation of a soluble protein.
Removal of protein dissolved in water by batch foam separation was conducted with using ovalbumin (OA) as a model protein in the light of wastewater treatment reducing organic loading. The removal efficiency had a maximum value near the i.e.p. of OA (pH 4.6); thus, most experiments were conducted at pH 4.6. Typical experimental conditions; superficial gas velocity, U(g): 1.97 x 10(-2)-5.37 x 10(-2)cm/s; initial bulk concentration of OA, C(i): ca. 0.05-0.25 g/L; liquid volume, V: 600 cm(3). A model estimating bulk concentration profile was proposed by taking into account a mass balance of the present system. The model predicted that OA could be removed perfectly, however, was not all removed experimentally. The residual OA concentration of the bulk liquid within the column reached plateau value, which correspond to ca. 18% of the initial OA concentration. The plateau value of the bulk concentration was attained for ca. 100-500 min with U(g)=1.97 x 10(-2)-5.37 x 10(-2)cm/s. Foaming ability test revealed that the foaming limit concentration of OA at pH 4.6 was 9.72 x 10(-3)g/L. These results suggested that OA molecules could be damaged by interaction of bubble surface in the dispersed phase, since there were the residual OA concentrations over the limit concentration. To take account of this phenomena and correct the model, average surface density, X(d), which should convert protein molecule into the denatured protein molecule, was introduced. The corrected model could explain well the time profile of OA bulk concentration.
Maruyama
Hideo
H
Division of Marine Biosciences, Graduate School of Fisheries Sciences, Hokkaido University, Minato 3-1-1, Hakodate 041-8611, Japan. maruyama@elsie.fish.hokudai.ac.jp
Seki
Hideshi
H
Suzuki
Akira
A
Inoue
Norio
N
D006863:Hydrogen-Ion Concentration; D010047:Ovalbumin / Q000737:chemistry / Q000302:isolation & purification; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012995:Solubility; D014865:Waste Disposal, Fluid / Q000295:instrumentation / Q000379:methods*
D011506:Proteins; D010047:Ovalbumin