Cyclosporine-induced apoptosis in osteosarcoma cells.
BACKGROUND
Posttransplant bone disease is one of the complications of cyclosporine (CsA), which is widely used as an immunosuppressive agent in the field of kidney transplantation. Cyclosporine treatment causes osteopenia as a result of altered bone turnover, but the pathogenic mechanisms of this process remain unclear. This study examined the ability of CsA to induce apoptosis in a rat osteoblast cell line.


RESULTS
We induced apoptosis in rat osteoblastic ROS 17/2.8 cells by exposure to CsA. MTT assay showed that CsA exhibited significant cytotoxic effects on ROS 17/2.8 cells in a dose-dependent manner. Western blot analysis showed enhanced processing of caspase-8, Bax, and p53 after CsA treatment. Expression of cleaved poly (ADP-ribose) polymerase (PARP) was elevated by CsA treatment. Pro-caspase-3 and Bcl-2 proteins were decreased by CsA.


CONCLUSIONS
These results suggested that CsA induced apoptosis of osteoblasts.
Oh
Y L
YL
Department of Obstetrics and Gynecology, Kosin University Medical College, Pusan, Korea.
Han
S Y
SY
Mun
K H
KH
Choi
H J
HJ
Kim
H Y
HY
Hwang
E A
EA
Park
S B
SB
Kim
H C
HC
Chang
E J
EJ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D012516:Osteosarcoma; D051381:Rats
D007166:Immunosuppressive Agents; D016572:Cyclosporine