Expression of delta1-pyrroline-5-carboxylate synthetase gene during drought in rice (Oryza sativa L.).
The 45-days-old seedlings of drought resistant (N-22, CR143-2-2) and susceptible rice (Oryza sativa L.) genotypes (Panidhan, Pusa-169) were subjected to osmotic stress in PEG-6000 solution of -10 and -16 bar and the relative water content (RWC), proline content, and pyrroline-5-carboxylate synthetase (P5CS) activity and its P5CS expression were studied. A gradual decrease in RWC was observed in tolerant genotypes, whereas the decrease was drastic in susceptible ones. Proline content and P5CS activity increased both in susceptible and tolerant genotypes; the increase was higher in tolerant genotypes. Higher proline levels in tolerant genotypes were due to increased P5CS activity. The EcoRI, BamHI and XbaI restricted DNA of N-22 and Panidhan genotypes were hybridized with Arabidopsis P5CS sequence and a single band (approx 2.4 kb) was observed, however, P5CS expression was more in N-22 as compared to Panidhan.
Choudhary
N L
NL
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi.
Sairam
R K
RK
Tyagi
A
A
D050842:1-Pyrroline-5-Carboxylate Dehydrogenase / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D015152:Blotting, Northern; D015139:Blotting, Southern; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000201:enzymology / Q000235:genetics; D011392:Proline / Q000096:biosynthesis; D036226:Seedlings / Q000201:enzymology / Q000235:genetics; D014867:Water / Q000378:metabolism*
D014867:Water; D011392:Proline; D050842:1-Pyrroline-5-Carboxylate Dehydrogenase