title: alpha-Defensins from blood leukocytes of the monkey Papio hamadryas.
abstract: Three antimicrobial peptides named PHD1-3 (Papio hamadryas defensin) have been isolated from hamadryas baboon blood leukocytes using preparative electrophoresis and reverse-phase HPLC. The primary structures of these peptides have been determined by automated Edman degradation and mass-spectrometry. The results suggest that the peptides belong to the alpha-defensin family. Structural homology analysis reveals that among alpha-defensins from other animal species, PHD3 is the most closely related to RMAD5 (rhesus macaque alpha-defensin) (90% homology) from rhesus macaque leukocytes and also highly similar to human alpha-defensin HD5 (60% homology), which is produced by intestinal Paneth cells. The homology of PHD3 with human neutrophil alpha-defensin HNP1 (human natural peptide) was 30%. The primary structures of PHD1 and PHD2 are most similar to RED1 (rhesus enteral defensin), one of six enteral alpha-defensins of rhesus monkeys. PHD1-3 have been shown to be active against the Gram-positive bacteria Listeria monocytogenes and Staphylococcus aureus, the Gram-negative bacterium Escherichia coli, and the fungus Candida albicans, similarly to the human HNP1 defensin.
authors.affiliation: Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, 197376, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Tsvetkova
authors.forename: G M
authors.initials: GM
authors.lastname: Aleshina
authors.forename: O V
authors.initials: OV
authors.lastname: Shamova
authors.forename: L E
authors.initials: LE
authors.lastname: Leonova
authors.forename: R I
authors.initials: RI
authors.lastname: Lehrer
authors.forename: V N
authors.initials: VN
authors.lastname: Kokryakov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002176:Candida albicans / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000737:chemistry; D008089:Listeria monocytogenes / Q000187:drug effects; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D048529:Papio hamadryas / Q000097:blood* / Q000235:genetics; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000187:drug effects; D023084:alpha-Defensins / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D023084:alpha-Defensins