Genotoxic and antioxidant activities of ethoxyquin salts evaluated by the comet assay.
Four newly synthesized salts of ethoxyquin (EQ: 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline), an antioxidant used in animal feeds, were evaluated with the use of the comet assay performed on human lymphocytes: ethoxyquin ascorbate, ethoxyquin hexanoate, ethoxyquin salicylate and ethoxyquin salt of Trolox C (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid). In the study the abilities of these compounds to cause DNA fragmentation and to protect against H2O2-induced DNA damage were analysed. The obtained results were compared with those noted earlier for EQ. After EQ salts treatments (1-25 microM) the genotoxic effects were observed, but the genotoxic potentials of the compounds studied were lower than that of EQ. On the other hand, EQ salts, similarly to EQ, effectively protected the cells from oxidative effect of H2O2. EQ hexanoate was the most effective and its antioxidant activity was even slightly higher than that of EQ. We suggest that it is worth further detailed studies to estimate its usefulness as a preservative.
Błaszczyk
Alina
A
Department of Cytogenetics and Plant Molecular Biology, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland. ablasz@biol.uni.lodz.pl
Skolimowski
Janusz
J
Materac
Alicja
A
D000975:Antioxidants / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics; D005015:Ethoxyquin / Q000494:pharmacology* / Q000633:toxicity*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity*; D012492:Salts / Q000494:pharmacology
D000975:Antioxidants; D009153:Mutagens; D012492:Salts; D005015:Ethoxyquin; D006861:Hydrogen Peroxide