Phylogenetic analysis of human parvovirus B19, indicating two subgroups of genotype 1 in Vietnamese patients.
Recently, three distinct genotypes (1, 2 and 3) of human parvovirus B19 (B19) have been identified. However, the characteristics and distribution of B19 genotypes in Vietnam have not been investigated. Phylogenetic analysis using 49 subgenomic NS1/VP1u regions and two coding NS1-VP1/VP2 regions has been applied to investigate the prevalence of B19 genotypes in Vietnamese patients co-infected with Hepatitis B virus. Genetic analysis of the subgenomic NS1/VP1u region of B19 revealed that two genotypes of B19 were identified in these populations, with predominance of genotype 1 (47/49, 96 %) followed by genotype 2 (2/49, 4 %), but not genotype 3. Further, phylogenetic analysis of subgenomic B19 genomes revealed two major subgroups within genotype 1 (B19-1A and B19-1B) with an estimated nucleotide difference of >5 % between each subgroup, forming different branches. The mean percentage of amino acid variation between subgroup B19-1A and B19-1B was >2 % of the NS1, VP1 and VP2 proteins. Our results indicated that two of the three known genotypes of B19 were present in Vietnamese patients, with genotype 1 predominating, and that this genotype can be classified into at least two subgroups, B19-1A and B19-1B.
Toan
Nguyen L
NL
Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Germany.|Department of Molecular Pathology, Institute of Pathology, University Hospital of Tübingen, 72076 Tübingen, Germany.
Duechting
Anja
A
Department of Molecular Pathology, Institute of Pathology, University Hospital of Tübingen, 72076 Tübingen, Germany.
Kremsner
Peter G
PG
Medical Research Unit, Albert Schweitzer Hospital, Lambarene, Gabon.|Department of Parasitology, Institute of Tropical Medicine, University of Tübingen, Germany.
Song
Le H
LH
Tran Hung Dao Hospital, Hanoi, Vietnam.
Ebinger
Martin
M
Department of Pediatric Oncology, Children's Hospital, University of Tübingen, Germany.
Aberle
Susanne
S
Department of Molecular Pathology, Institute of Pathology, University Hospital of Tübingen, 72076 Tübingen, Germany.
Binh
Vu Q
VQ
354 Hospital, Hanoi, Vietnam.
Duy
Dinh Ng
DN
Tran Hung Dao Hospital, Hanoi, Vietnam.
Torresi
Joseph
J
Department of Medicine, Victorian Infectious Diseases Service, Centre for Clinical Research Excellence, Royal Melbourne Hospital, The University of Melbourne, Australia.
Kandolf
Reinhard
R
Department of Molecular Pathology, Institute of Pathology, University Hospital of Tübingen, 72076 Tübingen, Germany.
Bock
C-Thomas
CT
Department of Molecular Pathology, Institute of Pathology, University Hospital of Tübingen, 72076 Tübingen, Germany.
D000595:Amino Acid Sequence; D001483:Base Sequence; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000821:virology*; D016732:Parvovirus B19, Human / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D014744:Vietnam / Q000453:epidemiology