Moderate overexpression of AIB1 triggers pre-neoplastic changes in mammary epithelium.
Here we report a new model of pre-clinical breast cancer which has been generated by overexpressing the steroid receptor coactivator AIB1 at moderate levels in breast epithelium. Transgenic female mice display mammary hyperplasia at the onset of puberty, consistent with enhanced proliferation of primary mammary epithelial cultures and augmented levels of cyclin D1 and E-cadherin. Studies of BrdU incorporation revealed that AIB1 localizes to the nucleus during or after S phase, implicating a new role for AIB1 in cell-cycle progression subsequent to G1. Our findings suggest that moderate overexpression of AIB1 may represent one of the pre-neoplastic changes in breast tissue.
Avivar
Alvaro
A
Laboratory of Cellular and Molecular Biology, Centro de Investigacion Principe Felipe, CIPF, Autopista del Saler, 16, 46013 Valencia, Spain.
García-Macias
María Carmen
MC
Ascaso
Emma
E
Herrera
Guadalupe
G
O'Connor
José-Enrique
JE
Font de Mora
Jaime
J
D021581:Active Transport, Cell Nucleus / Q000235:genetics; D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D016193:G1 Phase / Q000235:genetics; D015870:Gene Expression; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006965:Hyperplasia / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D056921:Nuclear Receptor Coactivator 3; D016196:S Phase / Q000235:genetics; D012741:Sexual Maturation / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D015820:Cadherins; D015534:Trans-Activators; D019938:Cyclin D1; D051548:Histone Acetyltransferases; C538867:Ncoa3 protein, mouse; D056921:Nuclear Receptor Coactivator 3