title: In vitro study of lysozyme in poly(lactide-co-glycolide) microspheres with sucrose acetate isobutyrate.
abstract: This study investigated the suitability of microsphere formulations for extended protein delivery and complete protein release. These microspheres were prepared by a multi-emulsion method and prepared using a mixture of poly(lactide-co-glycolide) (PLGA), RG 502H (lactide:glycolide=50:50, M(W) 9300) and sucrose acetate isobutyrate (SAIB). SAIB embedded into the microspheres and mixed with PLGA, improved the efficiency of enzyme encapsulation. The in vitro release rate of lysozyme (Lys) from the microspheres was reduced due to the high viscosity of the added SAIB and less degradation of PLGA by SAIB. These properties enabled prolonged release of Lys for up to 2 months, characterized by a minimal initial burst of Lys and nearly zero-order protein release kinetics result from co-administration of sorbitan monooleate 80. When it is considered that degradation products of SAIB are inactive for labile proteins, SAIB may be regarded as a promising candidate for long-acting protein delivery.
authors.affiliation: Pharmaceutical & Health Research Institute, Amore Pacific Corporation/R&D Center, 314-1, Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-729, Republic of Korea. hejulu@hanmail.net
authors.forename: Eun Seong
authors.initials: ES
authors.lastname: Lee
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kwon
authors.forename: Hyeok
authors.initials: H
authors.lastname: Lee
authors.forename: Kun
authors.initials: K
authors.lastname: Na
authors.forename: Jung Ju
authors.initials: JJ
authors.lastname: Kim
mesh_terms: D003692:Delayed-Action Preparations; D006016:Glycolates; D006820:Hyaluronic Acid; D019344:Lactic Acid; D008837:Micrococcus / Q000187:drug effects; D008863:Microspheres; D009113:Muramidase / Q000008:administration & dosage* / Q000494:pharmacology; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013213:Starch; D013395:Sucrose / Q000031:analogs & derivatives*; D013997:Time Factors
chemical_list: D003692:Delayed-Action Preparations; D006016:Glycolates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D013395:Sucrose; D006820:Hyaluronic Acid; D013213:Starch; D009113:Muramidase; C002885:sucrose acetate isobutyrate