Rice embryogenic calli express a unique set of microRNAs, suggesting regulatory roles of microRNAs in plant post-embryogenic development.
In vitro cultured embryogenic callus was employed as a model to investigate microRNAs (miRNAs) associated with embryogenesis and post-embryonic development. Thirty-one miRNAs including 16 novel species were identified from a large number of small RNAs which were cloned from both differentiated and undifferentiated rice embryogenic calli. Four target genes of the miRNAs were further validated. A set of the miRNAs, including miR397 and miR156, exhibited intriguing expression patterns during the transition from undifferentiated to differentiated calli. By exploiting the correlations between the differential expression patterns of these miRNAs and their targets, the regulatory roles of the miRNAs on meristem maintenance and embryogenesis were indicated.
Luo
Yu-Chun
YC
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Zhongshan University, Guangzhou 510275, PR China.
Zhou
Hui
H
Li
Yan
Y
Chen
Jun-Yu
JY
Yang
Jian-Hua
JH
Chen
Yue-Qin
YQ
Qu
Liang-Hu
LH
D001483:Base Sequence; D003001:Cloning, Molecular; D047108:Embryonic Development; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000196:embryology* / Q000235:genetics / Q000254:growth & development*; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D018626:Retroelements / Q000235:genetics
D035683:MicroRNAs; D012333:RNA, Messenger; D018626:Retroelements