title: Occurrence of iridoid glycosides in in vitro cultures and intact plants of Scrophularia nodosa L.
abstract: Shoot, root, and callus cultures of Scrophularia nodosa L. (Scrophulariaceae) were established and cultivated in vitro. Iridoid glycosides, such as harpagoside, aucubin, and catalpol were identified by LC-ESI-MS and their contents determined by HPLC. For comparison intact plants of S. nodosa were analysed. In shoot cultures slightly lower amounts of detectable iridoid glycosides (4.36% dry weight) were determined than in the field grown plants (4.88%). Concentration of harpagoside was highest in leaves of field plants (1.05%) and in flowers of in vitro plantlets (1.10%). For aucubin the highest amount was found in the leaves of in vitro plantlets (1.67%) whereas the levels of aucubin in the leaves of field plants were remarkably lower. Catalpol was produced as a trace compound in intact plants and shoot cultures. Callus and root cultures were apparently not able to synthesise iridoid glycosides.
authors.affiliation: Institut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Sesterhenn
authors.forename: Melanie
authors.initials: M
authors.lastname: Distl
authors.forename: Michael
authors.initials: M
authors.lastname: Wink
mesh_terms: D003470:Culture Media; D006027:Glycosides / Q000378:metabolism*; D039823:Iridoids / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D018520:Plant Shoots / Q000378:metabolism*; D032266:Scrophularia / Q000378:metabolism*; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media; D006027:Glycosides; D039823:Iridoids