title: Silica-guanidinium thiocyanate-based nucleic acid isolation protocol does not improve sensitivity of two commercial tests for detection of Mycobacterium tuberculosis in respiratory samples.
authors.affiliation: Department of Medical Microbiology, Room L1-245, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100DE, Amsterdam, The Netherlands. h.r.vandoorn@amc.uva.nl
authors.forename: H R
authors.initials: HR
authors.lastname: van Doorn
authors.forename: E S
authors.initials: ES
authors.lastname: Bruijnesteijn van Coppenraet
authors.forename: B
authors.initials: B
authors.lastname: Duim
authors.forename: C M J E
authors.initials: CM
authors.lastname: Vandenbroucke-Grauls
authors.forename: J F
authors.initials: JF
authors.lastname: Weel
authors.forename: J
authors.initials: J
authors.lastname: Dankert
authors.forename: E J
authors.initials: EJ
authors.lastname: Kuijper
authors.forename: M D
authors.initials: MD
authors.lastname: de Jong
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D006146:Guanidines / Q000737:chemistry*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D009696:Nucleic Acids / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013183:Sputum / Q000382:microbiology; D013861:Thiocyanates / Q000737:chemistry*; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D006146:Guanidines; D009696:Nucleic Acids; D013861:Thiocyanates; C054436:guanidine thiocyanate; D012822:Silicon Dioxide