title: In vivo insertional mutagenesis in Corynebacterium pseudotuberculosis: an efficient means to identify DNA sequences encoding exported proteins.
abstract: The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport subunits and for hypothetical and unknown-function proteins.
authors.affiliation: Laboratório de Genética Celular e Molecular, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Fernanda A
authors.initials: FA
authors.lastname: Dorella
authors.forename: Estela M
authors.initials: EM
authors.lastname: Estevam
authors.forename: Luis G C
authors.initials: LG
authors.lastname: Pacheco
authors.forename: Cláudia T
authors.initials: CT
authors.lastname: Guimarães
authors.forename: Ubiraci G P
authors.initials: UG
authors.lastname: Lana
authors.forename: Eliane A
authors.initials: EA
authors.lastname: Gomes
authors.forename: Michele M
authors.initials: MM
authors.lastname: Barsante
authors.forename: Sérgio C
authors.initials: SC
authors.lastname: Oliveira
authors.forename: Roberto
authors.initials: R
authors.lastname: Meyer
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport; D016925:Corynebacterium pseudotuberculosis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004251:DNA Transposable Elements; D013293:Enterococcus faecalis / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D000469:Alkaline Phosphatase