Failure to detect human herpes simplex virus, cytomegalovirus, and Epstein-Barr virus viral genomes in giant cell arteritis biopsy specimens by real-time quantitative polymerase chain reaction.
A study provided evidence of human herpes simplex virus (HSV) DNA in giant cell arteritis (GCA) biopsy specimens. This prompted us to study our own GCA biopsy specimens using real-time quantitative polymerase chain reaction for the detection of HSV1, cytomegalovirus, and Epstein-Barr virus DNAs. Our study failed to confirm an association between HSV1 and GCA, revealing no viral genome in 35 biopsy specimens of histologically positive temporal arteries.
Cankovic
Milena
M
Department of Pathology, Henry Ford Hospital, Detroit, MI 48202, USA. Electronic address: mcankov1@hfhs.org.
Zarbo
Richard J
RJ
Department of Pathology, Henry Ford Hospital, Detroit, MI 48202, USA.
D001706:Biopsy; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis; D004279:DNA, Viral / Q000302:isolation & purification*; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis; D013700:Giant Cell Arteritis / Q000821:virology*; D006561:Herpes Simplex / Q000175:diagnosis; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018139:Simplexvirus / Q000302:isolation & purification*
D004279:DNA, Viral