Gene expression profiling of human synovial sarcoma cell line (Hs701.T) in response to IL-1beta stimulation.
OBJECTIVE
Synovial sarcoma (SS) is a malignant mesenchymal tumor that accounts for 5-10% of all soft tissue sarcoma. IL-1beta, a pleiotrophic cytokine, has been found in the tumor microenvironment which plays crucial roles in the pathogenesis of tumors.


METHODS
In this study, we used Hs701.T as a cellular model to study the short-term (4-h) and long-term (48-h) stimulatory effect of IL-1beta on cell proliferation and differential gene expression.


RESULTS
The results showed that IL-1beta can stimulate cell proliferation through activation of NF-kappaB and AP-1 transcription factors; sequentially triggers the expression of genes related to tumor progression. The microarray data indicated that most of the up-regulated genes were related to tumor progression. Five candidate genes which are involved in the mediation of proliferation (IL-6), apoptosis (Hsp27 and Daxx), and angiogenesis (PlGF and SPARC) were further validated by RT-PCR.


CONCLUSIONS
These findings may be useful for understanding the pathogenesis of synovial sarcoma.
Ha
W Y
WY
Hung Lai Ching Laboratory of Biomedical Science, Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong.
Li
X J
XJ
Yue
P Y K
PY
Wong
D Y L
DY
Yue
K K M
KK
Chung
W S
WS
Zhao
L
L
Leung
P Y
PY
Liu
L
L
Wong
R N S
RN
D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D018450:Disease Progression; D005347:Fibroblasts / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism*; D009369:Neoplasms / Q000098:blood supply; D009389:Neovascularization, Pathologic; D020411:Oligonucleotide Array Sequence Analysis; D013584:Sarcoma, Synovial / Q000378:metabolism*; D013583:Synovial Membrane / Q000473:pathology*; D013997:Time Factors
D053583:Interleukin-1beta