title: A dynamic expression survey identifies transcription factors relevant in mouse digestive tract development.
abstract: Tissue-restricted transcription factors (TFs), which confer specialized cellular properties, are usually identified through sequence homology or cis-element analysis of lineage-specific genes; conventional modes of mRNA profiling often fail to report non-abundant TF transcripts. We evaluated the dynamic expression during mouse gut organogenesis of 1381 transcripts, covering nearly every known and predicted TF, and documented the expression of approximately 1000 TF genes in gastrointestinal development. Despite distinctive structures and functions, the stomach and intestine exhibit limited differences in TF genes. Among differentially expressed transcripts, a few are virtually restricted to the digestive tract, including Nr2e3, previously regarded as a photoreceptor-specific product. TFs that are enriched in digestive organs commonly serve essential tissue-specific functions, hence justifying a search for other tissue-restricted TFs. Computational data mining and experimental investigation focused interest on a novel homeobox TF, Isx, which appears selectively in gut epithelium and mirrors expression of the intestinal TF Cdx2. Isx-deficient mice carry a specific defect in intestinal gene expression: dysregulation of the high density lipoprotein (HDL) receptor and cholesterol transporter scavenger receptor class B, type I (Scarb1). Thus, integration of developmental gene expression with biological assessment, as described here for TFs, represents a powerful tool to investigate control of tissue differentiation.
authors.affiliation: Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA.
authors.forename: Michael Y
authors.initials: MY
authors.lastname: Choi
authors.forename: Anthony I
authors.initials: AI
authors.lastname: Romer
authors.forename: Michael
authors.initials: M
authors.lastname: Hu
authors.forename: Maina
authors.initials: M
authors.lastname: Lepourcelet
authors.forename: Ambili
authors.initials: A
authors.lastname: Mechoor
authors.forename: Ayce
authors.initials: A
authors.lastname: Yesilaltay
authors.forename: Monty
authors.initials: M
authors.lastname: Krieger
authors.forename: Paul A
authors.initials: PA
authors.lastname: Gray
authors.forename: Ramesh A
authors.initials: RA
authors.lastname: Shivdasani
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental; D007422:Intestines / Q000737:chemistry / Q000196:embryology*; D051379:Mice; D008817:Mice, Mutant Strains; D038081:Organogenesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D014157:Transcription Factors