title: Detection, characterization and identification of crucifer phytoalexins using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry.
abstract: We have analyzed 23 crucifer phytoalexins (e.g. brassinin, dioxibrassinin, cyclobrassinin, brassicanals A and C) by HPLC with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) using both negative and positive ion modes. Positive ion mode ESI-MS appeared more sensitive than negative ion mode ESI-MS in detecting this group of compounds. A new HPLC separation method, new LC-MS and LC-MS(2) data and proposed fragmentation pathways, LC retention times, and UV spectra for selected compounds are reported.
authors.affiliation: Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, SK S7N 5C9, Canada. s.pedras@usask.ca
authors.forename: M Soledade C
authors.initials: MS
authors.lastname: Pedras
authors.forename: Adewale M
authors.initials: AM
authors.lastname: Adio
authors.forename: Mojmir
authors.initials: M
authors.lastname: Suchy
authors.forename: Denis P O
authors.initials: DP
authors.lastname: Okinyo
authors.forename: Qing-An
authors.initials: QA
authors.lastname: Zheng
authors.forename: Mukund
authors.initials: M
authors.lastname: Jha
authors.forename: Mohammed G
authors.initials: MG
authors.lastname: Sarwar
mesh_terms: D019607:Brassicaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012717:Sesquiterpenes; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013729:Terpenes / Q000032:analysis* / Q000737:chemistry; D000094162:Phytoalexins
chemical_list: D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins