G protein beta2 subunit interacts directly with neuropathy target esterase and regulates its activity.
Neuropathy target esterase (NTE) was identified as the primary target of organophosphate compounds that cause a delayed neuropathy with degeneration of nerve axons. NTE is a novel phospholipase B anchored to the cytoplasmic face of endoplasmic reticulum and essential for embryonic and nervous development. However, little is known about the regulation of NTE. A human fetal brain cDNA library was screened for proteins that interact with NTE, Gbeta2 and Gbeta2-like I subunits were found to be able to bind the C-terminal of NTE in yeast. The interaction of Gbeta2 and NTE was confirmed by in vivo co-immunoprecipitation analysis in COS7 cells. Furthermore, depletion of Gbeta2 by RNA interference down regulated the activity of NTE but not its expression level. In addition, the activity of NTE was down regulated by the G protein signal pathway influencing factor, pertussis toxin, treatment in vivo. These findings suggest that Gbeta2 may play a significant role in maintaining the activity of NTE.
Chen
Rui
R
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China.
Chang
Ping-An
PA
Long
Ding-Xin
DX
Liu
Cheng-Yun
CY
Yang
Lin
L
Wu
Yi-Jun
YJ
D000818:Animals; D019556:COS Cells; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D002522:Chlorocebus aethiops; D015536:Down-Regulation; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008245:Lysophospholipase / Q000096:biosynthesis* / Q000235:genetics; D009420:Nervous System / Q000196:embryology / Q000201:enzymology; D011485:Protein Binding; D034622:RNA Interference; D015398:Signal Transduction / Q000502:physiology*
D002265:Carboxylic Ester Hydrolases; C021859:neurotoxic esterase; D008245:Lysophospholipase; D019204:GTP-Binding Proteins