Phytochemical and genetic analysis in selected chemotypes of Withania somnifera.
The main active components and genetic profile of 15 selected accessions of Withania somnifera Dunal. were analysed. Ethanolic extract of the dried roots/leaves of the plant was concentrated under pressure at 50+/-5 degrees C and was analysed for main compounds (withanolides and withaferin A) by HPLC. All the main components were found to be present in accessions (AGB 002, AGB 009, RSS 009, RSS 033). Correlation of these main components with their genetic factors, was undertaken using AFLP (amplified fragment length polymorphism) markers. Among 64 primers 7 yielded optimum polymorphism. A total of 913 polymorphic peaks were generated using these primers. Jaccard's similarity coefficient indicated that accessions having almost the same active compounds clustered together. The present study demonstrates that AFLP can be successfully used to resolve the correlation of AFLP data with the presence of secondary metabolites.
Dhar
Rekha S
RS
Regional Research Laboratory, RRL, Biotechnology Division, Canal Road, Jammu, Tawi 180001, India.
Verma
Vijeshwar
V
Suri
Krishan A
KA
Sangwan
Rajinder S
RS
Satti
Naresh K
NK
Kumar
Arun
A
Tuli
Rakesh
R
Qazi
Gulam N
GN
D002851:Chromatography, High Pressure Liquid; D016172:DNA Fingerprinting; D005819:Genetic Markers; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics; D011110:Polymorphism, Genetic; D032341:Withania / Q000737:chemistry* / Q000235:genetics
D005819:Genetic Markers; D010936:Plant Extracts