Immunohistochemical detection of p27 and p21 proteins in canine hair follicle and epidermal neoplasms.
Trichoblastomas, trichoepitheliomas, and squamous cell carcinomas in the skin of dogs were analysed by immunohistochemistry for the nuclear expression of p27, p21 and proliferating cell nuclear antigen (PCNA). High levels of p27 were present in trichoepitheliomas and trichoblastomas compared with squamous cell carcinomas. Detectable p21 was found in trichoepitheliomas and squamous cell carcinomas, but trichoblastomas had low level of p21 nuclear reactivity. Low levels of PCNA were detected in trichoepitheliomas and trichoblastomas compared with squamous cell carcinomas. The results suggested that nuclear p27 acts as a cyclin-dependent kinase (CDK) inhibitor in trichoepitheliomas and trichoblastomas. Nuclear p21 expression is involved in the induction of epithelial differentiation and seems to be unrelated to CDK inhibition.
Inoue
Makoto
M
Department of Veterinary Pathology, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
Wu
Haiyan
H
Une
Satoshi
S
D000818:Animals; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism*; D004283:Dog Diseases / Q000378:metabolism; D004285:Dogs; D004817:Epidermis / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D018859:Hair Follicle / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology
D050759:Cyclin-Dependent Kinase Inhibitor p21; D018809:Proliferating Cell Nuclear Antigen; C413374:p27 antigen