Experimental approaches to identify genetic networks.
Systems biology offers the promise of a fully integrated view of cellular physiology. To realize this potential requires the analysis of diverse genome-wide datasets and the incorporation of these analyses into integrated networks. In the past decade, the budding yeast Saccharomyces cerevisiae has provided the benchmark for the design of such large-scale experiments. Many of these experimental approaches have been adopted and adapted to study other systems, including worm, fly, fish and mammalian cultured cells, using an ingenious set of molecular tools.
Costanzo
Michael
M
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.
Giaever
Guri
G
Nislow
Corey
C
Andrews
Brenda
B
D000818:Animals; D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D005821:Genetic Techniques; D016678:Genome / Q000235:genetics*; D006801:Humans; D008962:Models, Theoretical; D025941:Protein Interaction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism