[Early marker of myocardial injury of the ischemia-reperfused heart in dogs and during operations with artificial circulation in humans].
The model of ischemia/reperfusion was reproduced on 9 unconscious dogs. Simultaneously with registration of indexes heart work, hemodynamic and mitochondrial factor (MF) in venous blood from right atrium was defined. Measures were done by spectrophotometria. We have also performed spectrophotometric determination of MF in 11 patients in course of operation with blood cardioplegia. Samples of mixed venous blood from the right atrium were taken on different stages of artificial blood circulation: ischemia and reperfusion. Besides that, patients' level of enzymes was defined: creatine kinase (CK), mv-creatine kinase (mv-CK), lactatedehydrogenase (LDG), aspartataminotransferase (AST), within first day of postoperative period, and also ECG-recording within pre- and after operative period were done. Maximal MF level correlates with frequency and severity of cardiac rhythm disturbance, severity of myocardial hypoxia (r = 0.81). Maximum MF level also demonstrated correlation with KK (r = 0.97), mvKK (r = 0.92), LDG(r = 0.81), AST (r = 0.85). Thus, in experimental and clinic conditions myocardial ischemia was accompanied by mPTP activation, which led to reperfusion myocardial injuries and to release of MF. Method of MF determination gives opportunity to propose its usage as early marker of ischemic injuries and also as marker of mPTP opening in vivo.
Sahach
V F
VF
Maksymenko
V B
VB
Dmytriieva
A V
AV
Bubnova
Iu O
IuO
Bohuslavs'kyĭ
A Iu
AIu
Knyshev
H V
HV
D000328:Adult; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D003326:Coronary Circulation / Q000502:physiology*; D003402:Creatine Kinase / Q000097:blood; D004285:Dogs; D004562:Electrocardiography; D005112:Extracorporeal Circulation; D006324:Heart Arrest, Induced / Q000009:adverse effects*; D006325:Heart Atria / Q000378:metabolism / Q000503:physiopathology; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008875:Middle Aged; D008929:Mitochondria, Heart / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000097:blood / Q000209:etiology / Q000503:physiopathology; D010101:Oxygen Consumption / Q000502:physiology
D015415:Biomarkers; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase