Effect of fungal polysaccharides on the modulation of plasma proteins in streptozotocin-induced diabetic rats.
To search out novel biomarkers for monitoring diabetes prognosis, we examined the effect of hypoglycemic fungal exopolysaccharides (EPS) on the differential levels of plasma proteins in streptozotocin-induced diabetic rats. The orally administrated EPS exhibited an excellent hypoglycemic effect, lowering the average plasma glucose level, and increasing insulin secretion in diabetic rats. The 2-DE analysis of rat plasma demonstrated that about 500 visualized spots were differentially regulated, of which 20 spots were identified as principal diabetes-associated proteins. The distinct effect of diabetes induction on the pattern of rat plasma proteins includes the down-regulation of albumin, apolipoprotein E (Apo E), alpha1-inhibitor-3, fetuin beta, Gc-globulin, hemopexin, vitronectin, and transthyretin (TTR) monomer, and the up-regulation of Apo A-I, Apo A-IV, ceruloplasmin, alpha1-antitrypsin, serine protease inhibitor III, and transferrin. Those protein levels were interestingly restored to those of healthy rats by EPS treatment, although the order of magnitude of the changes differed widely. Two proteins of interest showed distinct differential expression with opposite trends: TTR tetramer was significantly down-regulated and immunoglobulin (Ig) kappa light chain was significantly up-regulated upon diabetes induction, both of which were also normalized to those of healthy groups after EPS treatment.
Kim
Sang Woo
SW
Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, Korea.
Hwang
Hye Jin
HJ
Kim
Hyun Mi
HM
Lee
Myung Chul
MC
Shik Lee
Myung
M
Choi
Jang Won
JW
Yun
Jong Won
JW
D000595:Amino Acid Sequence; D000818:Animals; D001487:Basidiomycota / Q000737:chemistry / Q000502:physiology*; D001798:Blood Proteins / Q000096:biosynthesis*; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D015180:Electrophoresis, Gel, Two-Dimensional; D007003:Hypoglycemia / Q000097:blood; D008297:Male; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000502:physiology*; D020543:Proteome / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin
D001798:Blood Proteins; D011134:Polysaccharides; D020543:Proteome; D013311:Streptozocin