title: Failure to detect human herpes simplex virus, cytomegalovirus, and Epstein-Barr virus viral genomes in giant cell arteritis biopsy specimens by real-time quantitative polymerase chain reaction.
abstract: A study provided evidence of human herpes simplex virus (HSV) DNA in giant cell arteritis (GCA) biopsy specimens. This prompted us to study our own GCA biopsy specimens using real-time quantitative polymerase chain reaction for the detection of HSV1, cytomegalovirus, and Epstein-Barr virus DNAs. Our study failed to confirm an association between HSV1 and GCA, revealing no viral genome in 35 biopsy specimens of histologically positive temporal arteries.
authors.affiliation: Department of Pathology, Henry Ford Hospital, Detroit, MI 48202, USA. Electronic address: mcankov1@hfhs.org.
authors.forename: Milena
authors.initials: M
authors.lastname: Cankovic
authors.affiliation: Department of Pathology, Henry Ford Hospital, Detroit, MI 48202, USA.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Zarbo
mesh_terms: D001706:Biopsy; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis; D004279:DNA, Viral / Q000302:isolation & purification*; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis; D013700:Giant Cell Arteritis / Q000821:virology*; D006561:Herpes Simplex / Q000175:diagnosis; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018139:Simplexvirus / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral