title: SDS-CGE of proteins in microchannels made of SU-8 films.
abstract: This work describes the SDS-CGE of proteins carried out in microchannels made of the negative photoresist EPON SU-8. Embedded electrophoretic microchannels have been fabricated with a multilayer technology based on bonding and releasing steps of stacked SU-8 films. This technology allows the monolithic integration of the electrodes in the device. A high wafer fabrication yield and mass production compatibility guarantees low costs and high reliability. A poly(methyl methacrylate) (PMMA) packaging allows an easy setup and replacement of the device for electrophoresis experiments. In addition, the wire-bonding step is avoided. The electrophoretic mobilities of four proteins have been measured in microchannels filled with polyacrylamide. Different pore sizes have been tested obtaining their Ferguson plots. Finally, a separation of two proteins (20 and 36 kDa) has been carried out confirming that this novel device is suitable for protein separation. A resolution of 2.75 is obtained. This is the first time that this SU-8 microfluidic technology has been validated for SDS-CGE of proteins. This technology offers better separation performance than glass channels, at lower costs and with an easy packaging procedure.
authors.affiliation: MEMS/MST Department, IKERLAN-IK4 S. Coop., Mondragón, Spain. magirregabiria@ikerlan.es
authors.forename: Maria
authors.initials: M
authors.lastname: Agirregabiria
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Blanco
authors.forename: Javier
authors.initials: J
authors.lastname: Berganzo
authors.forename: Asier
authors.initials: A
authors.lastname: Fullaondo
authors.forename: Ana María
authors.initials: AM
authors.lastname: Zubiaga
authors.forename: Kepa
authors.initials: K
authors.lastname: Mayora
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Ruano-López
mesh_terms: D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008904:Miniaturization; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D012680:Sensitivity and Specificity
chemical_list: D011506:Proteins; D019904:Polymethyl Methacrylate