Determining chorionicity in twin gestations: three-dimensional (3D) multiplanar sonographic measurement of intra-amniotic membrane thickness.
OBJECTIVE
Measuring the thickness of the membranes to determine chorionicity in twins using two-dimensional (2D) ultrasound may provide uncertain results, given the possibility of measuring in an oblique view. We aimed to determine whether chorionicity can be assessed during the second and third trimesters by measuring the thickness of the membranes with three-dimensional (3D) multiplanar ultrasound, which ensures the measurement is perpendicular to the membranes, and to evaluate the reproducibility of the technique.


METHODS
This was a prospective study of 84 twin pregnancies (30 monochorionic and 54 dichorionic) between 20 and 35 weeks of gestation. The dividing membranes between the fetuses were visualized with 2D and 3D transabdominal sonography and the thickness of the membranes was measured by the multiplanar technique. Intraobserver variability was assessed by comparing the measurements obtained in sagittal and axial views by the same observer. We examined the frequency distributions to determine the optimal cut-off point of the thickness of the membranes to predict chorionicity. Kappa index, intraclass correlation coefficients and 95% limits of agreement were calculated to evaluate the inter- and intraoperator variability. We also compared membrane-thickness measurement, during the second and third trimesters, using 2D and 3D ultrasound.


RESULTS
In monochorionic and dichorionic pregnancies, respectively, the mean thickness was 1.42 (SD, 0.31) mm and 2.48 (SD, 0.47) mm. With 3D ultrasound, the intraclass correlation coefficient was 0.99 in all cases. The 95% limits of agreement were all within +/-0.2 mm. The best cut-off for membrane thickness for discriminating monochorionic from dichorionic twinning was 1.8 mm. Using this cut-off, there were one false-negative (sensitivity, 97%; 95% CI, 83-99%) and three false-positive (specificity, 94%; 95% CI, 85-99%) diagnoses of monochorionic twins. Kappa indices for intra- and interoperator variability were all above 90%, suggesting almost perfect agreement. Measurements with 2D sonography were less accurate (sensitivity and specificity, 83%).


CONCLUSIONS
Prenatal assessment of dividing membranes using 3D multiplanar ultrasound is reproducible and may be a useful alternative method for determining chorionicity in twin pregnancies during the second and third trimesters.
Senat
M-V
MV
Department of Obstetrics and Gynecology, Université Paris-XI, CHU Clamart, France. marie-victoire.senat@abc.aphp.fr
Quarello
E
E
Levaillant
J M
JM
Buonumano
A
A
Boulvain
M
M
Frydman
R
R
D000328:Adult; D000650:Amnion / Q000033:anatomy & histology / Q000000981:diagnostic imaging*; D002823:Chorion / Q000196:embryology*; D005260:Female; D005865:Gestational Age; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D011272:Pregnancy, Multiple; D011446:Prospective Studies; D015203:Reproducibility of Results; D016216:Ultrasonography, Prenatal / Q000379:methods*