Elevated temperature and temperature programming in conventional liquid chromatography--fundamentals and applications.
Temperature, as a powerful variable in conventional LC is discussed from a fundamental point of view and illustrated with applications from the author's laboratory. Emphasis is given to the influence of temperature on speed, selectivity, efficiency, detectability, and mobile phase composition (green chromatography). The problems accompanying the use of elevated temperature and temperature programming in LC are reviewed and solutions are described. The available stationary phases for high temperature operation are summarized and a brief overview of recent applications reported in the literature is given.
Vanhoenacker
Gerd
G
Research Institute for Chromatography, Kortrijk, Belgium.
Sandra
Pat
P
D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013696:Temperature