title: Chromatin immunoprecipitation reveals a novel role for the Drosophila SoxNeuro transcription factor in axonal patterning.
abstract: In all metazoans, the expression of group B HMG domain Sox transcription factors is associated with the earliest stages of CNS development. In Drosophila, SoxNeuro (SoxN) is involved in dorso-ventral patterning of the neuroectoderm, and in the formation and segregation of neuroblasts. In this report, we show that SoxN expression persists in a subset of neurons and glial cells of the ventral nerve cord at embryonic stages 15/16. In an attempt to address SoxN function in late stages of CNS development, we have used a chromatin immunoprecipitation approach to isolate genomic regions bound in vivo by SoxN. We identified several genes involved in the regulation of axon scaffolding as potential direct target genes of SoxN, including beat1a, semaphorin2a, fasciclin2, longitudinal lacking and tailup/islet. We present genetic evidence for a direct involvement of SoxN in axonal patterning. Indeed, overexpressing a transcriptionally hyperactive mutated SoxN protein in neurons results in specific defects in axon scaffolding, which are also observed in transheterozygous combinations of SoxN null mutation and mutations in its target genes.
authors.affiliation: Institut de Génétique Humaine, Centre National de la Recherche Scientifique UPR1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France. fgirard@igh.cnrs.fr
authors.forename: Franck
authors.initials: F
authors.lastname: Girard
authors.forename: Willy
authors.initials: W
authors.lastname: Joly
authors.forename: Jean
authors.initials: J
authors.lastname: Savare
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bonneaud
authors.forename: Conchita
authors.initials: C
authors.lastname: Ferraz
authors.forename: Florence
authors.initials: F
authors.lastname: Maschat
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D019521:Body Patterning; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D004330:Drosophila / Q000196:embryology* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006609:High Mobility Group Proteins / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D009457:Neuroglia / Q000502:physiology; D009474:Neurons / Q000502:physiology; D055747:SOX Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D029721:Drosophila Proteins; D006609:High Mobility Group Proteins; D055747:SOX Transcription Factors; C412366:SoxN protein, Drosophila; D014157:Transcription Factors