title: Angiogenic potential of CD44+ CD90+ multipotent CNS stem cells in vitro.
abstract: Although it is now clear that several subpopulations of neural stem cells (NSCs) exist during early development and adulthood, the angiogenic potential of NSCs remains a subject of debate. Here, we report that CD44(+) CD90(+) cells isolated from primary neurospheres can form vascular-tube structures in vitro. NSCs isolated from the mouse embryonic cortex formed neurospheres when cultured in serum-free medium containing 20ng/ml basic fibroblast growth factor (bFGF). CD44(+) CD90(+) cells were enriched from the neurospheres using an EPICS ALTRA flow cytometer, and antibodies against CD44 and CD90. The purified CD44(+) CD90(+) cells generated neurospheres, and differentiated into neurons and astrocytes. When the cells were inoculated into collagen gels and cultured with 20% fetal bovine serum plus bFGF for 7 days, vascular tube-like structures were formed. These results indicate that CD44(+) CD90(+) cells have the ability to generate neurospheres and to form vascular tubes.
authors.affiliation: Department of Pharmacology, Meiji Pharmaceutical University, Tokyo 204-8588, Japan. oishikz@my-pharm.ac.jp <oishikz@my-pharm.ac.jp>
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Oishi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ito-Dufros
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D002490:Central Nervous System / Q000098:blood supply* / Q000166:cytology / Q000378:metabolism*; D003094:Collagen; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D005782:Gels; D018960:Hyaluronan Receptors / Q000378:metabolism*; D051379:Mice; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D018800:Thy-1 Antigens / Q000378:metabolism*
chemical_list: D005782:Gels; D018960:Hyaluronan Receptors; D018800:Thy-1 Antigens; D003094:Collagen