title: [Sentinel lymph nodes identification in vulvar cancer--methods and technique].
abstract: OBJECTIVE
  To evaluate detection of sentinel lymph nodes (SLN) in squamous vulvar cancer with blue dye and 99mTc. The study describes technique of application, timing, management, detection rate (DR), specific side detection rate (SSDR) and false negative rate.
  
  
  METHODS
  Prospective clinical study.
  
  
  METHODS
  Department of Obstetrics and Gynecology, Charles University Prague, 2nd Medical Faculty, Teaching Hospital Motol.
  
  
  METHODS
  46 women with squamous cell carcinoma tumors stage I or II, <4 cm with no clinical suspect lymph nodes were included. Blue dye alone was used in 16 women and the combination of 99mTc and blue dye was used in 30 women. Radiocoloid 99mTc was applied 3-5 hours and blue dye 3-5 minutes prior to ingvinal incision.
  
  
  RESULTS
  We detected 88 SLN in 61 inguinal spaces. The detection rate in the blue dye group was 68.8 % (11 cases). One false negative SLN (6.3 %) appeared in this group. In blue dye+ 99mTc group detection rate was 100 % with no false negative SLN.
  
  
  CONCLUSIONS
  Detection of SLN in squamous vulvar cancer with the combination of 99mTc and blue dye was statistically significantly more effective than using the blue dye alone.
authors.affiliation: Department of Obstetrics and Gynecology, Charles University Prague, 2nd Medical Faculty, Teaching Hospital Motol.
authors.forename: L
authors.initials: L
authors.lastname: Rob
authors.forename: H
authors.initials: H
authors.lastname: Robová
authors.forename: M
authors.initials: M
authors.lastname: Pluta
authors.forename: P
authors.initials: P
authors.lastname: Strnad
authors.forename: J
authors.initials: J
authors.lastname: Kacírek
authors.forename: R
authors.initials: R
authors.lastname: Chmel
authors.forename: L
authors.initials: L
authors.lastname: Teslík
authors.forename: P
authors.initials: P
authors.lastname: Skapa
authors.forename: K
authors.initials: K
authors.lastname: Táborská
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000556:secondary*; D004396:Coloring Agents; D005188:False Negative Reactions; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D019275:Radiopharmaceuticals; D012394:Rosaniline Dyes; D021701:Sentinel Lymph Node Biopsy / Q000379:methods; D013668:Technetium Tc 99m Aggregated Albumin; D014846:Vulvar Neoplasms / Q000473:pathology*
chemical_list: D004396:Coloring Agents; D019275:Radiopharmaceuticals; D012394:Rosaniline Dyes; D013668:Technetium Tc 99m Aggregated Albumin; C008769:sulfan blue