title: Microbial transformation of androst-4-ene-3,17-dione by Beauveria bassiana.
abstract: The microbial transformation of androst-4-ene-3,17-dione (I) by the fungus Beauveria bassiana CCTCC AF206001 has been investigated using pH 6.0 and 7.0 media. Two hydroxylated metabolites were obtained with the pH 6.0 medium. The major product was 11alpha-hydroxyandrost-4-ene-3,17-dione (II) whereas the minor product was 6beta,11alpha-dihydroxyandrost-4-ene-3,17-dione (III). On the other hand, four hydroxylated and/or reduced metabolites were obtained with the pH 7.0 medium. The major product was 11alpha,17beta-dihydroxyandrost-ene-3-one (V) and the minor products were 17beta-hydroxyandrost-ene-3-one (IV), 6beta,11alpha,17beta-trihydroxyandrost-ene-3-one (VI) and 3alpha,11alpha,17beta-trihydroxy-5alpha-androstane (VII). The products were purified by chromatographic methods, and were identified on the basis of spectroscopic methods. This fungus strain is clearly an efficient biocatalyst for 11alpha-hydroxylation and reduction of the 17-carbonyl group.
authors.affiliation: College of Pharmacy, Wuhan University, Wuhan 430072, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Xiong
authors.forename: Qi
authors.initials: Q
authors.lastname: Wei
authors.forename: Hanmei
authors.initials: H
authors.lastname: Chen
authors.forename: Shouwen
authors.initials: S
authors.lastname: Chen
authors.forename: Wenjin
authors.initials: W
authors.lastname: Xu
authors.forename: Guofu
authors.initials: G
authors.lastname: Qiu
authors.forename: Shucai
authors.initials: S
authors.lastname: Liang
authors.forename: Xianming
authors.initials: X
authors.lastname: Hu
mesh_terms: D000735:Androstenedione / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D052982:Beauveria / Q000378:metabolism*; D015394:Molecular Structure
chemical_list: D000735:Androstenedione