A proteomic analysis during serial subculture and osteogenic differentiation of human mesenchymal stem cell.
Although previous studies have reported the effects of extensive subculturing on proliferation rates and osteogenic potential of human mesenchymal stem cells (hMSCs), the results remain controversial. The aim of our study was to characterize the proliferation and osteogenic potential of hMSCs during serial subculture, and also to identify proteins that are differentially regulated in hMSCs during serial subculture and osteogenic differentiation using proteome analysis. Here we show that the proliferation and osteogenic capacity of hMSCs decrease during serial subculturing. Several proteins were shown to be differentially regulated during serial subculture; among these the expression of T-complex protein 1 alpha subunit (TCP-1alpha), a protein known to be associated with cell proliferation, cell cycle, morphological changes, and apoptosis, gradually decreased during serial subculture. Among proteins that were differentially regulated during osteogenic differentiation, chloride intracellular channel 1 (CLIC1) was downregulated only during the early passages eukaryotic translation elongation factor, and acidic ribosomal phosphoprotein P0 was downregulated during the middle passages, while annexin V, LIM, and SH3 domain protein 1 (LASP-1), and 14-3-3 protein gamma (YWHAG) were upregulated during the later passage. These studies suggest that differentially regulated passage-specific proteins may play a role in the decrease of osteogenic differentiation potential under serial subculturing.
Sun
Hyun Jin
HJ
Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.
Bahk
Young Yil
YY
Choi
Yon Rak
YR
Shim
Jung Hye
JH
Han
Seung Hwan
SH
Lee
Jin Woo
JW
D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D020543:Proteome; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism
D015415:Biomarkers; D018118:Chloride Channels; C467578:Clic4 protein, rat; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D020543:Proteome; D012333:RNA, Messenger; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins