title: Agesamides A and B, bromopyrrole alkaloids from sponge Agelas species: application of DOSY for chemical screening of new metabolites.
abstract: Diffusion-ordered NMR spectroscopy (DOSY) is a versatile and powerful NMR technique and a noninvasive analytical method for mixture analysis that does not require prior physical separation of the analytes. In our search for new metabolites from natural resources, DOSY was applied for constituent analysis of crude bromopyrrole fractions separated from an Okinawan marine sponge Agelas sp. so that two new bromopyrrole alkaloids, agesamides A (1) and B (2), have been isolated. The structures and relative stereochemistry of 1 and 2 were elucidated from spectroscopic data.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Tsuda
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yasuda
authors.forename: Eri
authors.initials: E
authors.lastname: Fukushi
authors.forename: Jun
authors.initials: J
authors.lastname: Kawabata
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Sekiguchi
authors.forename: Jane
authors.initials: J
authors.lastname: Fromont
authors.forename: Jun'ichi
authors.initials: J
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010879:Piperazines / Q000737:chemistry*; D011161:Porifera / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D010879:Piperazines; D011758:Pyrroles; C520896:agesamide A; C520897:agesamide B