The intracellular domain of the human protocadherin hFat1 interacts with Homer signalling scaffolding proteins.
The cadherin superfamily protein Fat1 is known to interact with the EVH1 domain of mammalian Ena/VASP. Here we demonstrate that: (i) the scaffolding proteins Homer-3 and Homer-1 also interact with the EVH1 binding site of hFat1 in vitro, and (ii) binding of Homer-3 and Mena to hFat1 is mutually competitive. Endogenous Fat1 binds to immobilised Homer-3 and endogenous Homer-3 binds to immobilised Fat1. Both, endogenous and over-expressed Fat1 exhibit co-localisation with Homer-3 in cellular protrusions and at the plasma membrane of HeLa cells. As Homer proteins and Fat1 have been both linked to psychic disorders, their interaction may be of patho-physiological importance.
Schreiner
Dietmar
D
Department of Biology, University of Konstanz, AG Hofer, P.O. Box M648, D-78457 Konstanz, Germany. dietmar.schreiner@uni-konstanz.de
Müller
Kathrin
K
Hofer
H Werner
HW
D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008040:Genetic Linkage; D006367:HeLa Cells; D000072233:Homer Scaffolding Proteins; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D021381:Protein Transport / Q000235:genetics; D011618:Psychotic Disorders / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D000098662:Vasodilator-Stimulated Phosphoprotein
D015820:Cadherins; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; C502403:FAT1 protein, human; D000072233:Homer Scaffolding Proteins; D008840:Microfilament Proteins; D010750:Phosphoproteins; D025521:Tumor Suppressor Proteins; D000098662:Vasodilator-Stimulated Phosphoprotein