Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes.
The expression of heterologous proteins in microorganisms using genetic recombination is still the high point in the development and exploitation of modern biotechnology. People can produce bioactive proteins from relatively cheap culture medium instead of expensive extraction. Host cell systems for the expression of heterologous genes are generally prokaryotic or eukaryotic systems, both of which have inherent advantages and drawbacks. An optimal expression system can be selected only if the productivity, bioactivity, purpose, and physicochemical characteristics of the interest protein are taken into consideration, together with the cost, convenience and safety of the system itself. Here, we concisely review the most frequently used prokaryotic, yeast, insect and mammalian expression systems, as well as expression in eukaryote individuals. The merits and demerits of these systems are discussed.
Yin
Jiechao
J
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, 150030 Harbin, China.
Li
Guangxing
G
Ren
Xiaofeng
X
Herrler
Georg
G
D001709:Biotechnology / Q000379:methods / Q000639:trends; D003001:Cloning, Molecular; D005057:Eukaryotic Cells; D005822:Genetic Vectors; D011387:Prokaryotic Cells; D014176:Protein Biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
D011994:Recombinant Proteins