Antiproliferative effect of millimeter radiation on human erythromyeloid leukemia cell line K562 in culture: ultrastructural- and metabolic-induced changes.
In the present study we compared the proliferation behavior, the ultrastructural morphology and the glycolitic metabolism of K562 cells irradiated by low-power wide-band millimeter waves, with those of sham-exposed K562 cells (control), maintained in the same culture conditions. The gigaHertz radiation treatments, performed between 53-78 10(9) Hz, induced a noticeable inhibition of the cell proliferation that could be related to relevant ultrastructural changes. Such effects brought the irradiated cell system to lose the homeostasis and to trigger defense/reparatory mechanisms in order to reestablish a new steady state. (13)C-Nuclear magnetic resonance data on the kinetic of glucose metabolism demonstrated that the irradiated cells enhanced the glycolitic aerobic pathway, indicating that such system need to produce an extra-bioenergy. Most of the ATP synthesized served probably to perform the above processes resulting in a significant decrease of the proliferation rate without significant cell death increment.
Beneduci
A
A
Department of Chemistry, University of Calabria, Via P. Bucci Cubo 17/D 87036 Arcavacata di Rende (CS), Italy. beneduci@unical.it <beneduci@unical.it>
Chidichimo
G
G
Tripepi
S
S
Perrotta
E
E
Cufone
F
F
D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis / Q000528:radiation effects*; D006801:Humans; D020014:K562 Cells; D008657:Metabolic Clearance Rate / Q000528:radiation effects; D008872:Microwaves; D011829:Radiation Dosage
D005947:Glucose