A simple and reliable method to assess heart viability after hypothermic procurement.
Hearts from brain dead pigs (n = 18) were submitted to 0 (group I), 10 (group II), or 20 (group III) minutes of in situ warm ischemia (animal exsanguination). After harvesting, cold cardioplegia solution was perfused in retrograde fashion and initial coronary flow (ICF) measured. After left ventricular energetic indices were measured using NMR spectroscopy, the hearts were transplanted orthotopically. Follow-up was performed over 120 minutes after cardiopulmonary bypass. We observed a progressive decrease in ICF with increased warm ischemia times: 50 +/- 3.4 mL/min per 100 g of tissue in the group I, 36 +/- 7 and 30 +/- 3.5 in groups II and III, respectively (P < .05 and P < .01 versus group I). The ICF strongly correlated with the energetic index (r = 0.76, P < .001) and with posttransplant function of the transplanted heart. These data showed that measurement of initial coronary flow after cardioplegia was a reliable test to evaluate cardiac graft viability before transplantation.
Ferrera
R
R
INSERM EMI-U 0226, Laboratoire de Physiologie, Faculté de médecine Lyon-Nord, 8 avenue Rockefeller, 69373 Lyon Cedex 08, France. ferrera@lyon.inserm.fr
Bopassa
J-C
JC
Rodriguez
C
C
Baverel
G
G
Ovize
M
M
D000818:Animals; D001926:Brain Death; D002314:Cardioplegic Solutions; D003326:Coronary Circulation; D004487:Edema; D006321:Heart; D009926:Organ Preservation / Q000379:methods*; D013552:Swine; D016277:Ventricular Function, Left
D002314:Cardioplegic Solutions