Blood leukocyte mRNA expression for IL-10, IL-1Ra, and IL-8, but not IL-6, increases after exercise.
The primary purpose of this project was to study exercise-induced leukocyte cytokine mRNA expression. Changes in plasma cytokine levels and blood leukocyte mRNA expression for interleukin-6 (IL-6), IL-8, IL- 10, and IL-1 receptor antagonist (IL-1Ra) were measured in 12 athletes following 2 h of intensive cycling ( approximately 64% Watts(max)) while ingesting a carbohydrate or placebo beverage (randomized and double blinded). Blood samples were collected 30 min preexercise and immediately and 1 h postexercise. Carbohydate compared with placebo ingestion attenuated exercise-induced changes in plasma cortisol (8.8% vs. 62%, respectively), epinephrine (-9.2% vs. 138%), IL-6 (10-fold vs. 40-fold), IL-10 (8.9-fold vs. 26-fold, and IL-1Ra (2.1-fold vs. 5.6-fold). Significant time effects were measured for blood leukocyte IL-8 (2.4-fold increase 1 h postexercise), IL-10 (2.7-fold increase), IL-1Ra (2.2-fold increase), and IL-6 (0.8-fold decrease) mRNA content, with no significant differences between Cho and Pla test conditions. In summary, gene expression for IL-8, IL-10, and IL-1Ra, but not IL-6, is increased in blood leukocytes taken from athletes following 2 h of intensive cycling and is not influenced by carbohydrate compared with placebo ingestion. mRNA expression was high enough to indicate a substantial contribution of blood leukocytes to plasma levels of IL-8, IL-10, and IL-1Ra during prolonged exercise.
Nieman
David C
DC
Department of Health, Leisure, and Exercise Science, Biology, Family and Consumer Sciences, Fischer Hamilton/Nycom Biochemistry Laboratory, Appalachian State University, Boone, NC 28608, USA. niemande@appstate.edu
Henson
Dru A
DA
Davis
J Mark
JM
Dumke
Charles L
CL
Utter
Alan C
AC
Murphy
E Angela
EA
Pearce
Steven
S
Gojanovich
Greg
G
McAnulty
Steven R
SR
McAnulty
Lisa S
LS
D000328:Adult; D001642:Bicycling; D001786:Blood Glucose / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004837:Epinephrine / Q000097:blood; D015444:Exercise / Q000502:physiology*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007328:Insulin / Q000378:metabolism; D053590:Interleukin 1 Receptor Antagonist Protein / Q000097:blood / Q000235:genetics; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics; D016209:Interleukin-8 / Q000097:blood / Q000235:genetics; D007962:Leukocytes / Q000166:cytology / Q000502:physiology*; D008297:Male; D010919:Placebos; D012333:RNA, Messenger / Q000378:metabolism*; D014662:Vasoconstrictor Agents / Q000097:blood
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D007328:Insulin; D053590:Interleukin 1 Receptor Antagonist Protein; D015850:Interleukin-6; D016209:Interleukin-8; D010919:Placebos; D012333:RNA, Messenger; D014662:Vasoconstrictor Agents; D016753:Interleukin-10; D006854:Hydrocortisone; D004837:Epinephrine