title: AopP, a type III effector protein of Aeromonas salmonicida, inhibits the NF-kappaB signalling pathway.
abstract: Aeromonas salmonicida subsp. salmonicida contains a functional type III secretion system that is responsible for the secretion of the ADP-ribosylating toxin AexT. In this study, the authors identified AopP as a second effector protein secreted by this system. The aopP gene was detected in both typical and atypical A. salmonicida isolates and was found to be encoded on a small plasmid of approximately 6.4 kb. Sequence analysis indicates that AopP is a member of the YopJ family of effector proteins, a group of proteins that interfere with mitogen-activated protein kinase (MAPK) and/or nuclear factor kappa B (NF-kappaB) signalling pathways. AopP inhibits the NF-kappaB pathway downstream of IkappaB kinase (IKK) activation, while a catalytically inactivated mutant, AopPC177A, does not possess this inhibitory effect. Unlike other effectors of the YopJ family, such as YopJ and VopA, AopP does not inhibit the MAPK signalling pathway.
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
authors.forename: Désirée
authors.initials: D
authors.lastname: Fehr
authors.affiliation: Division of Molecular Pathology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
authors.forename: Carlo
authors.initials: C
authors.lastname: Casanova
authors.affiliation: Department of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Liverman
authors.affiliation: Division of Molecular Pathology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
authors.forename: Hana
authors.initials: H
authors.lastname: Blazkova
authors.affiliation: Department of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, USA.
authors.forename: Kim
authors.initials: K
authors.lastname: Orth
authors.affiliation: Division of Molecular Pathology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
authors.forename: Dirk
authors.initials: D
authors.lastname: Dobbelaere
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
authors.affiliation: Institute of Veterinary Bacteriology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Burr
mesh_terms: D048409:Aeromonas salmonicida / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016328:NF-kappa B / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D016328:NF-kappa B