Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins.
We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 A) than any previously obtained with biarsenical dyes.
Spagnuolo
Carla C
CC
Departamento de Química Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CIHIDECAR, CONICET, Ciudad Universitaria-Pabellón II, 1428, Buenos Aires, Argentina.
Vermeij
Rudolf J
RJ
Jares-Erijman
Elizabeth A
EA
D001152:Arsenicals / Q000737:chemistry*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis*; D004355:Drug Stability; D005452:Fluoresceins / Q000737:chemistry*; D005454:Fluorescence Polarization; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009842:Oligopeptides / Q000032:analysis; D010777:Photochemistry
D001152:Arsenicals; D005452:Fluoresceins; D006845:Hydrocarbons, Fluorinated; D009842:Oligopeptides; D003545:Cysteine