Overexpression of a member of the pentraxin family (PTX3) in human soft tissue liposarcoma.
A unique feature of human soft tissue liposarcoma is a stable (12;16)(q13;p11) translocation observed mainly in myxoid and roundcell liposarcomas. This translocation results in FUS/CHOP fusion transcripts with a corresponding oncogenic protein. We hypothesised that genes downstream of FUS/CHOP might serve as attractive candidates for novel tumour associated antigens. Among a panel of analysed genes, only pentraxin related gene (PTX3) demonstrated high expression in liposarcomas as compared to normal tissues. The analysis of RNA and protein expression demonstrated concordant results. However, the level of RNA and protein overexpression did not correlate in all cases. Finally, PTX3 expression was not related to presence of a FUS/CHOP fusion transcript within the liposarcoma tissues. PTX3 has been associated with adipocyte differentiation and now, additionally, is characterised by a markedly increased expression in human soft tissue liposarcoma. This finding mandates further research efforts to clarify the exact role of PTX3 in liposarcoma oncogenesis.
Willeke
F
F
Department of Surgery, Mannheim University Clinic, University of Heidelberg, Theodor Kutzer Ufer 1-3, 68135 Mannheim, Germany. frank.willeke@chir.ma.uni-heidelberg.de
Assad
A
A
Findeisen
P
P
Schromm
E
E
Grobholz
R
R
von Gerstenbergk
B
B
Mantovani
A
A
Peri
S
S
Friess
H H
HH
Post
S
S
von Knebel Doeberitz
M
M
Schwarzbach
M H M
MH
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002097:C-Reactive Protein / Q000378:metabolism*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008080:Liposarcoma / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D015514:Oncogene Proteins, Fusion / Q000378:metabolism; D034702:RNA-Binding Protein FUS / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000683:Serum Amyloid P-Component / Q000378:metabolism*; D051742:Transcription Factor CHOP / Q000378:metabolism; D014178:Translocation, Genetic; D000099153:Pentraxins
D002097:C-Reactive Protein; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D034702:RNA-Binding Protein FUS; D000683:Serum Amyloid P-Component; D051742:Transcription Factor CHOP; D000099153:Pentraxins; C106030:TLS-CHOP fusion protein, human