title: The ntp operon encoding the Na+ V-ATPase of the thermophile Caloramator fervidus.
abstract: The V-type ATPase of the thermophile Caloramator fervidus is an ATP-driven Na+ pump. The nucleotide sequence of the ntpFIKECGABD operon containing the structural genes coding for the nine subunits of the enzyme complex was determined. The identity of the proteins in two pairs of subunits (D, E and F, G) that have very similar mobilities on SDS-PAGE of the purified complex (24.3 and 22.7 kDa, and 12.3 and 11.6 kDa) was established by tryptic digestion of the protein bands followed by mass spectrometric analysis of the peptides.
authors.affiliation: Molecular Microbiology, Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN, Haren, Groningen, The Netherlands.
authors.forename: Trees
authors.initials: T
authors.lastname: Ubbink-Kok
authors.forename: Jeroen
authors.initials: J
authors.lastname: Nijland
authors.forename: Dirk-Jan
authors.initials: DJ
authors.lastname: Slotboom
authors.forename: Juke S
authors.initials: JS
authors.lastname: Lolkema
mesh_terms: D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D016937:Gram-Positive Endospore-Forming Rods / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D009876:Operon / Q000235:genetics*; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics* / Q000302:isolation & purification; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D046912:Multiprotein Complexes; D025262:Vacuolar Proton-Translocating ATPases; D000254:Sodium-Potassium-Exchanging ATPase