Identification of the Mycobacterium tuberculosis GlnE promoter and its response to nitrogen availability.
Adenylyltransferase, GlnE, has a predicted role in controlling the enzymic activity of glutamine synthetase, the key enzyme in ammonia assimilation. It was previously demonstrated that glnE is an essential gene in Mycobacterium tuberculosis. glnE is located downstream of glnA2, one of four glutamine synthetases. The expression of GlnE under various conditions was determined. Although a co-transcript of glnA2 and glnE was detectable, the major transcript was monocistronic. A transcriptional start site immediately upstream of glnE was identified and it was shown by site-directed mutagenesis that the predicted -10 region is a functional promoter. It was demonstrated that in a Mycobacterium smegmatis background M. tuberculosis P(glnE) was up-regulated in ammonia- or glutamine-containing media.
Pashley
Carey A
CA
Centre for Infectious Disease, Institute for Cell and Molecular Science, Barts and the London, London E1 2AT, UK.
Brown
Amanda C
AC
Centre for Infectious Disease, Institute for Cell and Molecular Science, Barts and the London, London E1 2AT, UK.
Robertson
Dina
D
Centre for Infectious Disease, Institute for Cell and Molecular Science, Barts and the London, London E1 2AT, UK.
Parish
Tanya
T
Centre for Infectious Disease, Institute for Cell and Molecular Science, Barts and the London, London E1 2AT, UK.
D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D020043:Genes, Essential; D016297:Mutagenesis, Site-Directed; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics; D009584:Nitrogen / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015854:Up-Regulation
D009713:Nucleotidyltransferases; C020658:glutamine-synthetase adenylyltransferase; D009584:Nitrogen