Tpc1808 increases expression of NF-H in PC12 cells.
Tpc1808 is a candidate chemotropic factor induced by nerve injury, and we report here the role of rat Tpc1808 in the expression of high-molecular-weight neurofilament (NF-H) in PC12 cells. A full-length Tpc1808 cDNA encoding the 275-amino-acid protein was constructed into pcDNA-HA and transfected into PC12 cells. The increased expression of NF-H was detectable in PC12-Tpc1808 clones by semi-qRT-PCR, real-time PCR, Western blot, and immunofluorescence. Such an increase could also be observed in PC12 cells subjected to recombinant Tpc1808 protein by real-time PCR and immunohistochemistry. At a concentration of 0.1 microg/mL, Tpc1808 protein, similar to NGF, could promote the expression of NF-H in a time-dependent manner. Our findings suggest that Tpc1808 is the gene related to promotion of nerve growth, and both the Tpc1808 gene and the Tpc1808 recombinant protein up-regulate the expression of NF-H in PC12 cells.
Liu
Mei
M
The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Peoples Republic of China.
Gu
Xiaosong
X
Zhang
Qi
Q
Liu
Yan
Y
Lin
Sheyu
S
Gu
Yun
Y
Ding
Fei
F
D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000235:genetics / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats
D036341:Intercellular Signaling Peptides and Proteins; D016900:Neurofilament Proteins; C051774:neurofilament protein H; D000581:Amidohydrolases; C518395:Tpc1808 protein, rat