title: A simple DNA stretching method for fluorescence imaging of single DNA molecules.
abstract: Stretching or aligning DNA molecules onto a surface by means of molecular combing techniques is one of the critical steps in single DNA molecule analysis. However, many of the current studies have focused on lambda-DNA, or other large DNA molecules. There are very few studies on stretching methodologies for DNA molecules generated via PCR (typically smaller than 20 kb). Here we describe a simple method of stretching DNA molecules up to 18 kb in size on a modified glass surface. The very low background fluorescence allows efficient detection of single fluorescent dye labels incorporated into the stretched DNA molecules.
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco CA, USA.
authors.forename: Ting-Fung
authors.initials: TF
authors.lastname: Chan
authors.forename: Connie
authors.initials: C
authors.lastname: Ha
authors.forename: Angie
authors.initials: A
authors.lastname: Phong
authors.forename: Dongmei
authors.initials: D
authors.lastname: Cai
authors.forename: Eunice
authors.initials: E
authors.lastname: Wan
authors.forename: Lucinda
authors.initials: L
authors.lastname: Leung
authors.forename: Pui-Yan
authors.initials: PY
authors.lastname: Kwok
authors.forename: Ming
authors.initials: M
authors.lastname: Xiao
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes; D005898:Glass / Q000737:chemistry; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D004247:DNA