title: Comparative proteomic analysis of mouse embryonic stem cells and neonatal-derived cardiomyocytes.
abstract: Pluripotent embryonic stem cells (ESCs) spontaneously differentiate via embryo-like aggregates into cardiomyocytes. A thorough understanding of the molecular conditions in ESCs is necessary before other potential applications of these cells such as cell therapy can be materialized. We applied two dimensional electrophoresis to analyze and compare the proteome profiling of spontaneous mouse ESC-derived cardiomyocytes (ESC-DCs), undifferentiated mouse ESCs, and neonatal-derived cardiomyocytes (N-DCs). Ninety-five percent of the proteins detected on the ESC-DCs and N-DCs could be precisely paired with one other, whereas only twenty percent of the ESC proteins could be reliably matched with those on the ESC-DCs and N-DCSs, suggesting a striking similarity between them. Having identified sixty proteins in the said three cell types, we sought to provide possible explanations for their differential expression patterns and discuss their relevance to cell biology. This study provides a new insight into the gene expression pattern of differentiated cardiomyocytes and is further evidence for a close relation between ESC-DCs and N-DCSs.
authors.affiliation: Department of Stem Cells, Royan Institute, Tehran, Iran. baharvand50@yahoo.com <baharvand50@yahoo.com>
authors.forename: Hossein
authors.initials: H
authors.lastname: Baharvand
authors.forename: Mohsen
authors.initials: M
authors.lastname: Hajheidari
authors.forename: Roseata
authors.initials: R
authors.lastname: Zonouzi
authors.forename: Saeid Kazemi
authors.initials: SK
authors.lastname: Ashtiani
authors.forename: Saman
authors.initials: S
authors.lastname: Hosseinkhani
authors.forename: Ghasem Hosseini
authors.initials: GH
authors.lastname: Salekdeh
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004622:Embryo, Mammalian / Q000737:chemistry / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D032383:Myocytes, Cardiac / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013234:Stem Cells / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020543:Proteome