The lack of inhibition of porcine endogenous retrovirus by small interference RNA designed from the long terminal regions.
Xenotransplantation from pigs may offer a potential solution to the organ shortage. However, there remains the risk of xenoinfection by porcine endogenous retroviruses (PERVs) that cannot be eliminated by breeding pigs under specified pathogen-free conditions. RNA interference is a new method to inhibit the expression of a specific gene. Here, we designed two siRNAs from the long terminal repeat of PERV. Our results showed that these siRNAs had no inhibitory effects. The possible reasons for this are an off-target effect or a problem with specific sequence of RNAi. Future work should focus on siRNAs from conserved regions of other PERV genes.
Li
Z
Z
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Guoxuexiang 37, Chengdu 610041, P.R. China.
Ping
Y
Y
Shengfu
L
L
Youping
L
L
Jingqiu
C
C
Hong
B
B
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D020077:Endogenous Retroviruses / Q000187:drug effects / Q000235:genetics*; D007668:Kidney; D034741:RNA, Small Interfering / Q000235:genetics / Q000494:pharmacology*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000821:virology*; D020079:Terminal Repeat Sequences; D014162:Transfection
D034741:RNA, Small Interfering; D012367:RNA, Viral