[Preparation and identification of rabbit antibody against LRR of Nogo-66 receptor (NgR)].
OBJECTIVE
To prepare rabbit antibody against LRR of Nogo-66 receptor (NgR) and identify its properties.


METHODS
LRR protein was expressed in E.coli BL21. Rabbits were immunized with purified LRR protein to prepare anti-LRR antibody. The titer and specificity of prepared antibodies were identified by Western blot and immunohistochemical staining, respectively.


RESULTS
Purified rabbit anti-rat LRR antibody with high titer was obtained. The anti-LRR Ab showed good specificity and could be used to detect the NgR in rat brain and spinal cord tissues. Immunohistochemical staining indicated that NgR was expressed widely in spinal cord neurons.


CONCLUSIONS
Successful preparation of anti-rat LRR antibody provides a useful tool for identification and further functional study of NgR molecule.
Yu
Pan-pan
PP
Department of Neurobiology, Medical School of Shanghai Jiaotong University, Shanghai 200025, China.
Zhu
Hui-qin
HQ
Lu
Pei-hua
PH
Huang
Li-dong
LD
D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000276:immunology*; D000918:Antibody Specificity; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D058851:GPI-Linked Proteins; D015870:Gene Expression; D008297:Male; D009185:Myelin Proteins / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology*; D000070816:Nogo Receptor 1; D011817:Rabbits; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology*; D020449:Repetitive Sequences, Amino Acid; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism
D000906:Antibodies; D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D000070816:Nogo Receptor 1; D011956:Receptors, Cell Surface; C486581:Rtn4r protein, rat