Role of IgG(T) and IgGa isotypes obtained from arachnidic antivenom to neutralize toxic activities of Loxosceles gaucho, Phoneutria nigriventer and Tityus serrulatus venoms.
The ability of IgG(T) and IgGa subclasses--isolated by liquid chromatography from equine arachnidic antivenom (AAV)-to neutralize toxic activities of Loxosceles gaucho, Phoneutria nigriventer and Tityus serrulatus venoms as well as to remove venom toxins from circulation was investigated. These subclasses showed similar antibody titers against L. gaucho, P. nigriventer and T. serrulatus venoms, and by immunoblotting few differences were observed in the recognition pattern of venom antigens. IgG(T) and IgGa neutralized 100% lethality induced by L. gaucho and 50% of P. nigriventer venom, but IgGa failed to neutralize T. serrulatus venom, in contrast to IgG(T). Both subclasses neutralized local reactions and dermonecrosis induced by L. gaucho venom in rabbits. In mice, IgG(T) and IgGa partially neutralized the edematogenic activity induced by P. nigriventer and T. serrulatus venoms, but only IgG(T) neutralized (ca. 81%) the nociceptive activity induced by T. serrulatus venom. Both subclasses failed to neutralize nociceptive activity induced by P. nigriventer venom. IgG(T) reduced the serum venom levels of animals injected with L. gaucho, P. nigriventer or T. serrulatus venoms, while IgGa solely reduced L. gaucho and P. nigriventer venoms levels. Our results demostrate that IgG(T) and IgGa subclasses neutralize toxic activities induced by P. nigriventer, T. serrulatus and L. gaucho venoms with different efficacies, as well as depurate these venoms from circulation.
Toro
Ana Flávia
AF
Laboratory of Immunopathology, Butantan Institute, Av. Vital Brazil 1500, 05503-900 São Paulo, SP, Brazil.
Malta
Marília Brinati
MB
Soares
Sabrina Lucio
SL
Da Rocha
Guilherme Casoni
GC
da Silva Lira
Marcela
M
De Oliveira
Thais Abbate
TA
Takehara
Harumi Ando
HA
Lopes-Ferreira
Mônica
M
Santoro
Marcelo Larami
ML
Guidolin
Rosalvo
R
Gondo Higashi
Hisako
H
Fernandes
Irene
I
Barbaro
Katia Cristina
KC
D000818:Animals; D000997:Antivenins / Q000737:chemistry / Q000494:pharmacology*; D002853:Chromatography, Liquid; D006736:Horses / Q000276:immunology; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D010727:Phosphoric Diester Hydrolases / Q000276:immunology / Q000633:toxicity; D012604:Scorpion Venoms / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000633:toxicity; D013111:Spider Venoms / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000633:toxicity; D013112:Spiders / Q000737:chemistry* / Q000378:metabolism
D000997:Antivenins; D007074:Immunoglobulin G; D012604:Scorpion Venoms; D013111:Spider Venoms; C055161:loxosceles venom; D010727:Phosphoric Diester Hydrolases