title: The potential of comparative genomic hybridization as a tool in the differential diagnosis of matrix-producing bone lesions.
abstract: Matrix-producing bone lesions consist of a wide variety of benign and malignant conditions. With respect to morphology, an overlap exists between benign and malignant bone tumors that causes difficulties in the final determination of the tumor. This study was conducted to show the potential of comparative genomic hybridization as a tool in the differential diagnosis of matrix-producing bone lesions. Thirty benign bone tumors were evaluated by conventional comparative genomic hybridization. To test its diagnostic reliability, 5 additional cases were analyzed, all with differential diagnostic difficulties related to morphology and radiology. All were ultimately diagnosed as malignant sarcomas, and unbalanced alterations were detected. In contrast benign tumors or tumor-like lesions did not reveal any chromosomal alterations. Comparative genomic hybridization is a useful adjunct in the complicated differential diagnostic algorithms of matrix-producing bone tumors.
authors.affiliation: Department of Orthopaedic Surgery, Muenster University Hospital, Muenster, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Gebert
authors.forename: Christian
authors.initials: C
authors.lastname: Brinkschmidt
authors.forename: Stefan
authors.initials: S
authors.lastname: Bielack
authors.forename: Thomas
authors.initials: T
authors.lastname: Bernhardt
authors.forename: Heribert
authors.initials: H
authors.lastname: Jürgens
authors.forename: Werner
authors.initials: W
authors.lastname: Böcker
authors.forename: Winfried
authors.initials: W
authors.lastname: Winkelmann
authors.forename: Horst
authors.initials: H
authors.lastname: Bürger
authors.forename: Georg
authors.initials: G
authors.lastname: Gosheger
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001859:Bone Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D002648:Child; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000235:genetics*; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization; D012516:Osteosarcoma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D004273:DNA, Neoplasm