Expression of the mRNA and protein of serine racemase in primary cultures of rat neurons.
Real-time quantitative PCR, Western blot and in situ hybridization techniques were employed to clarify the presence of serine racemase in the primary cultures of rat neurons. We have detected both serine racemase mRNA and protein in the cultured neurons. Both the mRNA and the protein levels in the neurons are higher than those in the astrocytes. Sequential detection of serine racemase mRNA and MAP2 immunoreactivity also revealed that serine racemase and MAP2 are co-localized in the cultured neurons. These data are the first to demonstrate that a substantial amount of serine racemase exists in the cultured neurons.
Yoshikawa
Masanobu
M
Department of Clinical Pharmacology, Tokai University School of Medicine, Isehara, Kanagawa 259-1143, Japan.
Nakajima
Kazuyuki
K
Takayasu
Naoko
N
Noda
Setsuko
S
Sato
Yuichi
Y
Kawaguchi
Mitsuru
M
Oka
Tetsuo
T
Kobayashi
Hiroyuki
H
Hashimoto
Atsushi
A
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002478:Cells, Cultured; D015870:Gene Expression; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D019998:Racemases and Epimerases / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D000099300:Serine Racemase
D012333:RNA, Messenger; D019998:Racemases and Epimerases; D000099300:Serine Racemase