[Expression map of immune-related genes on PBMCs in familial clustering patients with chronic virus hepatitis B].
OBJECTIVE
To investigate peripheral blood mononuclear cells (PBMCs) immune-related gene expression profile in patients with chronic virus hepatitis B(CHB) by oligonucleotide gene array technique.


METHODS
PBMCs were collected from the members of a family of clustering hepatitis B virus (HBV) infection including 5 CHB patients and 4 healthy spouses and RNA prepared from PBMCs was hybridized to high-density oligonucleotide arrays(HG-U133A 2.0 Human Gene Chips, Affymetrix), covering the expression of 22 000 human ESTs. Primary scanned image was analyzed with DNT software package.


RESULTS
Out of the 22 000 ESTs, 24 different immune-related genes were identified. Among the 24 genes, 7 genes showed increased expression and 17 genes showed decreased expression in CHB compared with those in healthy spouses. The up-regulated genes were mainly associated with adaptive immunity, while the down-regulated genes were associated with innate immunity.


CONCLUSIONS
Our findings suggest that HBV infection alters a broad range of immunity genes expression and innate immunity-associated genes are important in the defense against HBV chronic infection.
He
Ying-li
YL
Infectious Department, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. heyingli2000@163.com
Zhao
Ying-ren
YR
Zhang
Shu-lin
SL
Yang
Yuan
Y
Liu
Min
M
Jin
Yan
Y
Liu
Jin-feng
JF
D015536:Down-Regulation; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D019694:Hepatitis B, Chronic / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D010375:Pedigree; D015854:Up-Regulation