Transcription modulation of Salmonella enterica serovar Typhimurium promoters by sub-MIC levels of rifampin.
Promoter-lux fusions that showed rifampin-modulated transcription were identified from a Salmonella enterica serovar Typhimurium 14028 reporter library. The transformation of a subset of fusions into mutants that lacked one of six global regulatory proteins or were rifampin resistant showed that transcription modulation was independent of the global regulators, promoter specific, and dependent on the interaction of rifampin with RNA polymerase.
Yim
Grace
G
Department of Microbiology and Immunology, University of British Columbia, Life Sciences Institute, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada.
de la Cruz
Fernando
F
Spiegelman
George B
GB
Davies
Julian
J
D000904:Antibiotics, Antitubercular / Q000378:metabolism / Q000494:pharmacology*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D012293:Rifampin / Q000378:metabolism / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
D000904:Antibiotics, Antitubercular; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin