title: Evaluation of Th1-like, Th2-like and immunomodulatory cytokine mRNA expression in the skin of dogs with immunomodulatory-responsive lymphocytic-plasmacytic pododermatitis.
abstract: The term immunomodulatory-responsive lymphocytic-plasmacytic pododermatitis (ImR-LPP) has previously been proposed to denote a subpopulation of dogs with idiopathic pododermatitis. The objective of this study was to quantify the expression of mRNA encoding Th(1)-like [interferon (IFN)-gamma, interleukin (IL)-2 and IL-12], Th(2)-like [IL-4 and IL-6] and immunomodulatory cytokines [IL-10 and transforming growth factor (TGF)-beta] in lesional ImR-LPP, nonlesional ImR-LPP and healthy control pedal skin. Gene transcripts were quantified using TaqMan real-time reverse transcriptase-polymerase chain reaction assays. The skin of dogs with ImR-LPP had significant overexpression of IL-6 mRNA (P < 0.05) and significant underexpression of IL-12 mRNA (P < 0.01) compared to healthy controls. In addition, lesional ImR-LPP skin had significantly higher levels of IL-10 transcripts compared to healthy control pedal skin (P < 0.05). Although not attaining significance (P = 0.07), a trend towards reduced TGF-beta mRNA expression in lesional ImR-LPP skin was also evident. There were no significant differences in the levels of IFN-gamma or IL-2 mRNA transcripts among the three skin sample sources. IL-4 mRNA was detected in only one lesional sample. These results suggest that the pathogenesis of ImR-LPP may be associated with a T-cell-mediated inflammatory response characterized by impaired Th(1)-like, but enhanced Th(2)-like cytokine expression.
authors.affiliation: Department of Small Animal Clinical Studies, Faculty of Veterinary Medicine, University College Dublin, Dublin 4, Ireland. rory.breathnach@ucd.ie
authors.forename: Rory M
authors.initials: RM
authors.lastname: Breathnach
authors.forename: Shay
authors.initials: S
authors.lastname: Fanning
authors.forename: Grace
authors.initials: G
authors.lastname: Mulcahy
authors.forename: Hugh F
authors.initials: HF
authors.lastname: Bassett
authors.forename: Boyd R
authors.initials: BR
authors.lastname: Jones
authors.forename: Paul
authors.initials: P
authors.lastname: Daly
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D003872:Dermatitis / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D004283:Dog Diseases / Q000276:immunology* / Q000378:metabolism; D004285:Dogs; D005260:Female; D005534:Foot Diseases / Q000276:immunology / Q000378:metabolism / Q000662:veterinary; D005786:Gene Expression Regulation; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000276:immunology / Q000378:metabolism*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D016207:Cytokines; D012333:RNA, Messenger