Characterization of cellular retinoid-binding proteins in human myometrium during pregnancy.
Many complementary or competing signalling pathways bear an influence on the myometrium at any one time, and because the retinoic acid signalling pathway influences differentiation of a wide array of human tissues, this may be one of the determinants of myometrial differentiation during pregnancy. We have explored the novel hypothesis that the retinoids may act as important regulators in controlling the differentiated state of the human myometrium during pregnancy by characterizing the expression profiles for cellular retinoid-binding proteins CRBPI, CRABPI and CRABPII in non-pregnant, pregnant (non-labouring) and labouring human myometrium taken from the functionally distinct upper and lower uterine segments. In addition, we have investigated the effect of all-trans retinoic acid (ATRA) on the expression of several retinoic acid response genes including cyclooxygenase-2 (COX-2) and connexin-43 (Cx-43). Different spatial and temporal patterns of expression were observed for CRBPI, CRABPI and CRABPII within the upper and lower uterine segments through the three trimesters of pregnancy and in labour. Furthermore, the expression of COX-2, Cx-43, CRABPI, the transcription factor c-Jun and the retinoic acid receptor RARbeta altered in response to different concentrations of ATRA, suggesting that the differential expression of cellular retinoid-binding proteins may lead to different levels of retinoic acid being delivered to its nuclear targets, leading to the differential expression of specific target genes within the myometrium during pregnancy.
Tyson-Capper
Alison J
AJ
School of Surgical and Reproductive Sciences, The Medical School, University of Newcastle upon Tyne, UK. a.j.tyson-capper@ncl.ac.uk
Cork
David M W
DM
Wesley
Emily
E
Shiells
Elizabeth A
EA
Loughney
Andrew D
AD
D000328:Adult; D018031:Connexin 43 / Q000032:analysis; D051546:Cyclooxygenase 2 / Q000032:analysis; D005260:Female; D006801:Humans; D007743:Labor, Obstetric / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis; D009215:Myometrium / Q000737:chemistry* / Q000187:drug effects; D011247:Pregnancy / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis; D018168:Receptors, Retinoic Acid / Q000032:analysis*; D012176:Retinoids / Q000502:physiology*; D012177:Retinol-Binding Proteins / Q000032:analysis; D054840:Retinol-Binding Proteins, Cellular; D014212:Tretinoin / Q000494:pharmacology*
D018031:Connexin 43; C500216:GJA1 protein, human; D008565:Membrane Proteins; D016755:Proto-Oncogene Proteins c-jun; C518456:RBP1 protein, human; D018168:Receptors, Retinoic Acid; D012176:Retinoids; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; C082927:retinoic acid binding protein I, cellular; C082928:retinoic acid binding protein II, cellular; C082925:retinoic acid receptor beta; D014212:Tretinoin; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human