title: Increase in terminal restriction fragments of Bacteroidetes-derived 16S rRNA genes after administration of short-chain fructooligosaccharides.
abstract: It is well known that short chain fructooligosaccharides (scFOS) modify intestinal microbiota in animals as well as in humans. Since most murine intestinal bacteria are still uncultured, it is difficult for a culturing method to detect changes in intestinal microbiota after scFOS administration in a mouse model. In this study, we sought markers of positive change in murine intestinal microbiota after scFOS administration using terminal restriction fragment length polymorphism (T-RFLP) analysis, which is a culture-independent method. The T-RFLP profiles showed that six terminal restriction fragments (T-RFs) were significantly increased after scFOS administration. Phylogenetic analysis of the 16S rRNA partial gene sequences of murine fecal bacteria suggested that four of six T-RFs that increased after scFOS administration were derived from the 16S rRNA genes of the class Bacteroidetes. Preliminary quantification of Bacteroidetes by real-time PCR suggests that the 16S rRNA genes derived from Bacteroidetes were increased by scFOS administration. Therefore, the T-RFs derived from Bacteroidetes are good markers of change of murine intestinal microbiota after scFOS administration.
authors.affiliation: Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd., 5-3-1 Chiyoda, Sakado-shi, Saitama 350-0289, Japan. koichiro_murashima@meiji.co.jp.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakanishi
authors.forename: Koichiro
authors.initials: K
authors.lastname: Murashima
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ohara
authors.forename: Takahisa
authors.initials: T
authors.lastname: Suzuki
authors.forename: Hidenori
authors.initials: H
authors.lastname: Hayashi
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fukasawa
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kubota
authors.forename: Akira
authors.initials: A
authors.lastname: Hosono
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kono
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaminogawa
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Benno
mesh_terms: D000818:Animals; D041963:Bacteroidetes / Q000187:drug effects* / Q000235:genetics*; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D017753:Ecosystem; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D007422:Intestines / Q000187:drug effects / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000008:administration & dosage*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D019936:Probiotics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004040:Dietary Carbohydrates; D009844:Oligosaccharides; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C116580:fructooligosaccharide; C043600:fungitetraose