Gene therapy and transplantation in CNS repair: the visual system.
Normal visual function in humans is compromised by a range of inherited and acquired degenerative conditions, many of which affect photoreceptors and/or retinal pigment epithelium. As a consequence the majority of experimental gene- and cell-based therapies are aimed at rescuing or replacing these cells. We provide a brief overview of these studies, but the major focus of this review is on the inner retina, in particular how gene therapy and transplantation can improve the viability and regenerative capacity of retinal ganglion cells (RGCs). Such studies are relevant to the development of new treatments for ocular conditions that cause RGC loss or dysfunction, for example glaucoma, diabetes, ischaemia, and various inflammatory and neurodegenerative diseases. However, RGCs and associated central visual pathways also serve as an excellent experimental model of the adult central nervous system (CNS) in which it is possible to study the molecular and cellular mechanisms associated with neuroprotection and axonal regeneration after neurotrauma. In this review we present the current state of knowledge pertaining to RGC responses to injury, neurotrophic and gene therapy strategies aimed at promoting RGC survival, and how best to promote the regeneration of RGC axons after optic nerve or optic tract injury. We also describe transplantation methods being used in attempts to replace lost RGCs or encourage the regrowth of RGC axons back into visual centres in the brain via peripheral nerve bridges. Cooperative approaches including novel combinations of transplantation, gene therapy and pharmacotherapy are discussed. Finally, we consider a number of caveats and future directions, such as problems associated with compensatory sprouting and the reformation of visuotopic maps, the need to develop efficient, regulatable viral vectors, and the need to develop different but sequential strategies that target the cell body and/or the growth cone at appropriate times during the repair process.
Harvey
Alan R
AR
School of Anatomy and Human Biology, The University of Western Australia, Crawley, WA 6009, Australia.
Hu
Ying
Y
Leaver
Simone G
SG
Mellough
Carla B
CB
Park
Kevin
K
Verhaagen
Joost
J
Plant
Giles W
GW
Cui
Qi
Q
D000818:Animals; D017690:Cell Transplantation / Q000379:methods*; D002493:Central Nervous System Diseases / Q000150:complications / Q000628:therapy*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D009901:Optic Nerve Diseases / Q000209:etiology / Q000628:therapy*; D012165:Retinal Ganglion Cells / Q000637:transplantation; D016896:Treatment Outcome