FTMS and TOF/TOF mass spectrometry in concert: identifying peptides with high reliability using matrix prespotted MALDI target plates.
In this paper we describe a combination of the mass spectrometric techniques MALDI-TOF/TOF and MALDI-FTMS to identify proteins in complex samples using prespotted MALDI target plates. By this procedure accurate FTMS mass measurements and TOF/TOF data are obtained from the same spot. We have found that this combination of techniques leads to more reliable identification of peptides.
Dekker
Lennard J
LJ
Laboratory for Neuro-Oncology, Department of Neurology, Room AE312, Erasmus Medical Center, P.O. Box 1738, 3000DR Rotterdam, The Netherlands.
Burgers
Peter C
PC
Guzel
Coşkun
C
Luider
Theo M
TM
D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000097:blood* / Q000737:chemistry; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared
D001798:Blood Proteins; D010455:Peptides; D020543:Proteome