title: Enzymatic activation of lysine 2,3-aminomutase from Porphyromonas gingivalis.
abstract: The development of lysine 2,3-aminomutase as a robust biocatalyst hinges on the development of an in vivo activation system to trigger catalysis. This is the first report to show that, in the absence of chemical reductants, lysine 2,3-aminomutase activity is dependent upon the presence of flavodoxin, ferredoxin, or flavodoxin-NADP(+) reductase.
authors.affiliation: Biotechnology Development Center-Eddyville, Cargill Incorporated, 1 Cargill Drive, Eddyville, IA 52553, USA. brian_brazeau@cargill.com
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Brazeau
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Gort
authors.forename: Holly J
authors.initials: HJ
authors.lastname: Jessen
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Andrew
authors.forename: Hans H
authors.initials: HH
authors.lastname: Liao
mesh_terms: D002384:Catalysis; D004789:Enzyme Activation; D004926:Escherichia coli / Q000235:genetics; D005288:Ferredoxins / Q000235:genetics / Q000378:metabolism; D005418:Flavodoxin / Q000235:genetics / Q000378:metabolism; D019751:Intramolecular Transferases / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D005288:Ferredoxins; D005418:Flavodoxin; D011994:Recombinant Proteins; D009247:NADH, NADPH Oxidoreductases; C116041:flavodoxin NADPH oxidoreductase; D019751:Intramolecular Transferases; C021518:lysine 2,3-aminomutase