Effect of herbal extract mixture on menopausal urinary incontinence in ovariectomized rats.
The decline of estrogen production after menopause is contributing factor to urinary incontinence (UI), and particularly stress urinary incontinence (SUI). We determined the preventive effects of herbal extract mixture (HEM) on UI in ovariectomized Sprague Dawley rats. Female 9-weeks old rats were ovariectomized and treated with HEM (2.2, 11, or 55 mg/kg/day) for 8 weeks. The index of urinary bladder weight to body weight in the HEM and non-ovariectomized and non-treated (SHAM) groups were slightly higher than the ovariectomized, non-treated group (OVX). The contraction index of acetylcholine to KCl on detrusor smooth muscle strips in the HEM groups showed a dose-dependent recovery. HEM treatment also significantly improved collagen levels, as shown by Masson trichrome staining, as well as hydroxyproline levels in the urinary bladder. Serum estradiol levels in the HEM groups were higher than the OVX group. In conclusion, HEM increased estradiol levels in serum and improved factors related to urinary incontinence. The improvements in estradiol levels were related to changes in urinary incontinence.
Lee
Yong Hyuk
YH
Department of Hygienic Chemistry, College of Pharmacy, Kyung Hee University, Seoul, 130-701 Korea.
Hyun
Sun Hee
SH
Choung
Se Young
SY
D000109:Acetylcholine / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight / Q000502:physiology; D003094:Collagen / Q000187:drug effects / Q000378:metabolism; D028464:Cucurbita; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000097:blood; D005260:Female; D006909:Hydroxyproline / Q000187:drug effects / Q000378:metabolism; D007529:Isoflavones / Q000008:administration & dosage; D008593:Menopause / Q000652:urine*; D009929:Organ Size / Q000502:physiology; D010052:Ovariectomy; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max; D014549:Urinary Incontinence / Q000517:prevention & control*
D007529:Isoflavones; D010936:Plant Extracts; D004958:Estradiol; D003094:Collagen; D000109:Acetylcholine; D006909:Hydroxyproline