The rolC gene induces expression of a pathogenesis-related beta-1,3-glucanase in transformed ginseng cells.
The Agrobacterium rhizogenes rolC oncogene is capable of stimulating production of secondary metabolites in transformed plant cells that suggest its possible involvement in plant defense reactions. We tested whether the gene could also affect production of pathogenesis-related proteins. Using a well-known group of PR-proteins, such as beta-1,3-glucanases, we observed a 10-fold increase in total beta-1,3-glucanase activity in rolC-transformed Panax ginseng cells compared with normal cells. The increase was due to the production of a salicylic acid-activated beta-1,3-glucanase isoform. We isolated cDNA of the corresponding beta-1,3-glucanase gene (Pg-glu1), which shared 38-60% sequence identity with previously reported sequences of plant beta-1,3-glucanases at the protein level. Levels of Pg-glu1 mRNA transcripts were tightly correlated with expression of the rolC gene. Our data, together with previously reported information, indicate that A. rhizogenes can activate plant defense reactions via expression of T-DNA oncogenes.
Kiselev
Konstantin V
KV
Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, 159 Stoletija Strasse, Vladivostok 690022, Russia.
Kusaykin
Mikhail I
MI
Dubrovina
Alexandra S
AS
Bezverbny
Denis A
DA
Zvyagintseva
Tatiana N
TN
Bulgakov
Victor P
VP
D000085:Acetates / Q000494:pharmacology; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D043326:Glucan 1,3-beta-Glucosidase / Q000235:genetics* / Q000378:metabolism; D009857:Oncogenes; D054883:Oxylipins; D005894:Panax / Q000166:cytology / Q000201:enzymology / Q000235:genetics*; D010937:Plant Growth Regulators / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics*; D020156:Salicylic Acid / Q000494:pharmacology; D014170:Transformation, Genetic
D000085:Acetates; D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D010937:Plant Growth Regulators; D020033:Protein Isoforms; D012333:RNA, Messenger; C072239:methyl jasmonate; D043326:Glucan 1,3-beta-Glucosidase; D020156:Salicylic Acid