Comparison of Salmonella enterica serovar Typhimurium LT2 and non-LT2 salmonella genomic sequences, and genotyping of salmonellae by using PCR.
Genes of Salmonella enterica serovar Typhimurium LT2 expected to be specifically present in Salmonella were selected using the Basic Local Alignment Search Tool (BLAST) program. The 152 selected genes were compared with 11 genomic sequences of Salmonella serovars, including Salmonella enterica subsp. I and IIIb and Salmonella bongori (V), and were clustered into 17 groups by their comparison patterns. A total of 38 primer pairs were constructed to represent each of the 17 groups, and PCR was performed with various Salmonella subspecies including Salmonella enterica subsp. I, II, IIIa, IIIb, IV, VI, and V to evaluate a comprehensive DNA-based scheme for identification of Salmonella subspecies and the major disease-causing Salmonella serovars. Analysis of PCR results showed that Salmonella enterica subsp. I was critically divided from other subspecies, and Salmonella strains belonging to S. enterica subsp. I were clustered based on their serovars. In addition, genotypic relationships within S. enterica subsp. I by PCR results were investigated. Also, Salmonella signature genes, Salmonella enterica serovar Typhimurium signature genes, and Salmonella enterica subsp. I signature genes were demonstrated based on their PCR results. The described PCR method suggests a rapid and convenient method for identification of Salmonella serovars that can be used by nonspecialized laboratories. Genome sequence comparison can be a useful tool in epidemiologic and taxonomic studies of Salmonella.
Kim
Hyun-Joong
HJ
Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Suwon 449-701, Korea. hykim@khu.ac.kr
Park
Si-Hong
SH
Kim
Hae-Yeong
HY
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D005838:Genotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D012486:Salmonella typhimurium / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial