Antimicrobial activity of the methanolic extracts and compounds from Vismia laurentii De Wild (Guttiferae).
Methanolic extracts prepared from the leaves, twigs and the roots of Vismia laurentii De Wild as well as nine compounds isolated from these crude extracts, were tested for their antimicrobial activity against Gram-positive bacteria (six species), Gram-negative bacteria (12 species) and two Candida species using disc diffusion and well micro-dilution methods. The disc diffusion assay indicated that the crude extract was active against all the pathogens tested, whereas isolated compounds showed selective activities. The degree of sensitivity of the test organisms to purified compounds varied from 25 to 90%. Fridelin (8) was found to be the most active compound, while Bivismiaquinone (3) was the least active. The lowest minimum inhibition concentration (MIC) values as obtained by the micro-dilution assays were 19.53 and 1.22 microg/ml for the crude extracts and purified compounds, respectively. The lowest value for the purified compounds (1.22 microg/ml) was obtained with O(1)-demethyl-3',4'-deoxypsorospermin-3',4'-diol (6) on Candida gabrata and Bacillus subtilis; 1,8-dihydroxy-6-methoxy-3-methylanthraquinone (5) on Bacillus subtilis and 6-deoxyisojacareubin (7) on Bacillus stearothermophilus. These results provide promising baseline information for the potential use of these crude extracts as well as some of the isolated compounds in the treatment of bacterial and fungal infections.
Kuete
Victor
V
Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon. kuetevictor@yahoo.fr
Nguemeving
Jean Robert
JR
Beng
Véronique Penlap
VP
Azebaze
Anatole Guy Blaise
AG
Etoa
François-Xavier
FX
Meyer
Michèle
M
Bodo
Bernard
B
Nkengfack
Augustin Ephrem
AE
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D028084:Clusiaceae / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D000432:Methanol / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
D000890:Anti-Infective Agents; D010936:Plant Extracts; D000432:Methanol