Preliminary studies on production of extracellular lipase by an isolate from soil : Aspergillus flavus oryzae.
In our routine screening programme, using agar diffusion assay method, lipolytic activity was detected around a colony of a fungus. The fungus was isolated from a soil sample which was brought from a location near oil-mill. This lipolytic fungus was then identified to belong to Aspergillus flavus oryzae. A medium was then formulated and optimized which would not only support good growth but also would yield good extracellular lipolytic activity. It was observed that a conventional carbohydrate-protein containing medium supported good growth of the fungus but moderate lipase activity, whereas, a hydrocarbon containing medium, although supported relatively less growth, yielded much more lipase activity.
Venkatesh
A G
AG
Research and Development, Hindustan Antibiotics Ltd., Pimpri, Pune 411 018, India.
Muley
R G
RG
Naik
S R
SR
D001236:Aspergillus oryzae / Q000201:enzymology*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis*; D008670:Metals / Q000494:pharmacology; D012988:Soil Microbiology
D003470:Culture Media; D008670:Metals; D008049:Lipase