The replication clamp-loading machine at work in the three domains of life.
Sliding clamps are ring-shaped proteins that tether DNA polymerases to DNA, which enables the rapid and processive synthesis of both leading and lagging strands at the replication fork. The clamp-loading machinery must repeatedly load sliding-clamp factors onto primed sites at the replication fork. Recent structural and biochemical analyses provide unique insights into how these clamp-loading ATPase machines function to load clamps onto the DNA. Moreover, these studies highlight the evolutionary conservation of the clamp-loading process in the three domains of life.
Indiani
Chiara
C
Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, New York 10021, USA.
O'Donnell
Mike
M
D000251:Adenosine Triphosphatases / Q000378:metabolism; D017122:Bacteriophage T4 / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004261:DNA Replication / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D008954:Models, Biological; D008958:Models, Molecular; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D051818:Replication Protein C / Q000737:chemistry / Q000378:metabolism
D004269:DNA, Bacterial; D018809:Proliferating Cell Nuclear Antigen; D011506:Proteins; D004259:DNA-Directed DNA Polymerase; D000251:Adenosine Triphosphatases; D051818:Replication Protein C