rpoB Gene mutations and molecular characterization of rifampin-resistant Mycobacterium tuberculosis isolates from Shandong Province, China.
Sixty rifampin (RIF)-resistant and 75 RIF-susceptible Mycobacterium tuberculosis isolates from Shandong Province, China, were analyzed for rpoB gene mutations and genotyped. Mycobacterial interspersed repetitive unit (MIRU) genotype 223325173533 was overrepresented among RIF-resistant isolates. MIRU combined with IS6110 restriction fragment length polymorphism analysis as the second-line genotyping method may reflect epidemiologic links more reliably than each method alone.
Ma
Xin
X
Kathearine Hsu Internation REsearch Center of Human Infectious Diseases, Shandong Provinical Tuberculosis Control Center, Jinan, Shandong, China. xma@bcm.edu
Wang
Haiying
H
Deng
Yunfeng
Y
Liu
Zhimin
Z
Xu
Yong
Y
Pan
Xi
X
Musser
James M
JM
Graviss
Edward A
EA
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D002681:China; D004251:DNA Transposable Elements; D012321:DNA-Directed RNA Polymerases; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans; D020071:Interspersed Repetitive Sequences / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D012293:Rifampin / Q000494:pharmacology*; D018571:Sentinel Surveillance; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
D000904:Antibiotics, Antitubercular; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C505805:rpoB protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin