title: New phage type among methicillin-resistant Staphylococcus aureus associated with a local outbreak in Belgium during 2002.
abstract: In total, 150 methicillin-resistant Staphylococcus aureus (MRSA) isolates collected during 2002 from a general Belgian hospital were phage-typed at routine test dilution x 100. The majority (45%) belonged to phage group (J)*, while 10% were classified as a new phage type 29/(42E)/54/(D11)*. The isolates belonging to this new type carried the aac(6')-aph(2'') and the aph(3') aminoglycoside resistance genes and showed high-level resistance to oxacillin. Molecular typing revealed that they belonged to the multiresistant clonal pulsed-field gel electrophoresis (PFGE) type D8. PFGE group D, characterised as genotype ST228-MRSA-I, has been present in Belgian hospitals since 1999.
authors.affiliation: National Phage Typing Reference Centre, Pasteur Institute, Brussels, Belgium. cwildemauwe@pasteur.be
authors.forename: C
authors.initials: C
authors.lastname: Wildemauwe
authors.forename: C
authors.initials: C
authors.lastname: Godard
authors.forename: R
authors.initials: R
authors.lastname: Joseph
authors.forename: R
authors.initials: R
authors.lastname: De Ryck
authors.forename: A
authors.initials: A
authors.lastname: Deplano
authors.forename: S
authors.initials: S
authors.lastname: Damée
authors.forename: E
authors.initials: E
authors.lastname: Van Bossuyt
authors.forename: R
authors.initials: R
authors.lastname: Vanhoof
authors.forename: M
authors.initials: M
authors.lastname: Struelens
mesh_terms: D001434:Bacteriophage Typing; D001530:Belgium / Q000453:epidemiology; D004196:Disease Outbreaks; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification / Q000821:virology*