A modified Christensen's urea and CLSI broth microdilution method for testing susceptibilities of six Malassezia species to voriconazole, itraconazole, and ketoconazole.
Two supplemented broths (Christensen's urea with 0.1% Tween 80 and 0.5% Tween 40 and RPMI 1640 with 1% glycerol, 1% peptone, 1.8% glucose, and 0.05% Tween 80) were evaluated to determine voriconazole, itraconazole, and ketoconazole MICs for 200 Malassezia sp. isolates. Malassezia globosa and M. restricta were the least susceptible species (MICs at which 90% of the isolates tested were inhibited, 1 to >or=8 microg/ml versus 0.25 to 1 microg/ml).
Rincón
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Centro De Investigactiones Microbiológicas CIMIC, Universidad de los Andes, Bogotá, Columbia.
Cepero de García
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MC
Espinel-Ingroff
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A
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D000935:Antifungal Agents / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D003881:Dermatomycoses / Q000382:microbiology; D015658:HIV Infections / Q000150:complications; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology*; D007654:Ketoconazole / Q000494:pharmacology*; D008290:Malassezia / Q000145:classification / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D011136:Polysorbates; D011743:Pyrimidines / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*; D014508:Urea; D065819:Voriconazole
D000935:Antifungal Agents; D003470:Culture Media; D011136:Polysorbates; D011743:Pyrimidines; D014230:Triazoles; D017964:Itraconazole; D014508:Urea; D065819:Voriconazole; D007654:Ketoconazole