Separation of tricomponent protein mixtures with triblock nanorods.
Two-component triblock magnetic nanorods with gold end blocks and nickel interior blocks have been synthesized and used as affinity templates for the simultaneous and efficient separation of a three-component protein mixture. The gold blocks were selectively functionalized with 11-amino-1-undecanethiol, and then glutaraldehyde was used to covalently attach nitrostreptavidin to them. His-tagged proteins bind to the nickel block and biotin-tagged proteins bind to the functionalized gold ends, allowing one to separate a mixture of three proteins with a single material. Each surface-bound protein can be released selectively using imidazole for the His-tagged protein and biotin for the biotinylated protein.
Oh
Byung-Keun
BK
Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, Illinois 60208-3113, USA.
Park
Sungho
S
Millstone
Jill E
JE
Lee
Seung Woo
SW
Lee
Ki-Bum
KB
Mirkin
Chad A
CA
D000085:Acetates / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D002867:Chromones / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D006834:Hydrazines / Q000737:chemistry; D008280:Magnetics; D043942:Nanotubes / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D013205:Staphylococcal Protein A / Q000302:isolation & purification; D025801:Ubiquitin / Q000302:isolation & purification
D000085:Acetates; C400304:Alexa 350; C417561:Alexa 488 hydrazide; C417664:Alexa594; D002867:Chromones; D005456:Fluorescent Dyes; D006834:Hydrazines; D009930:Organic Chemicals; D011506:Proteins; D013205:Staphylococcal Protein A; D025801:Ubiquitin; D012710:Serum Albumin, Bovine; D006639:Histidine; D001710:Biotin; D006046:Gold; D009532:Nickel