title: A novel competitive fluorescence immunoassay for the determination of dibutyl phthalate.
abstract: A novel, sensitive, and specific competitive fluorescence immunoassay has been developed for the quantitative determination of dibutyl phthalate (DBP) using an antibody-coated plate format. Hapten was synthesized in order to produce polyclonal antibodies against dibutyl phthalate. Polyclonal antisera to dibutyl phthalate were generated in rabbits and used to construct the fluorescence immunoassay for measurement of dibutylphthalate. The assay had a detection limit of about 0.02 microg L(-1), a dynamic range of approximately 0.1-300 microg L(-1). Other similar phthalate compounds do not interfere significantly in the analysis using this immunoassay technique, and the cross-reactivity rates were less than 10%. The study demonstrated that the developed antiserum and fluorescence immunoassay procedure can be used to detect dibutyl phthalate in environmental samples such as tap water, river water, drinking water, and leachate from plastic drinking water bottles.
authors.affiliation: College of Environmental Science and Engineering, Donghua University, Shanghai 201620, People's Republic of China.
authors.forename: Ming-Cui
authors.initials: MC
authors.lastname: Zhang
authors.forename: Qiong-E
authors.initials: QE
authors.lastname: Wang
authors.forename: Hui-Sheng
authors.initials: HS
authors.lastname: Zhuang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D000937:Antigen-Antibody Reactions; D003993:Dibutyl Phthalate / Q000032:analysis*; D005260:Female; D005453:Fluorescence; D006241:Haptens / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods*; D009994:Osmolar Concentration; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D006241:Haptens; D003993:Dibutyl Phthalate