title: Revised model for Enterococcus faecalis fsr quorum-sensing system: the small open reading frame fsrD encodes the gelatinase biosynthesis-activating pheromone propeptide corresponding to staphylococcal agrd.
abstract: Gelatinase biosynthesis-activating pheromone (GBAP) is an autoinducing peptide involved in Enterococcus faecalis fsr quorum sensing, and its 11-amino-acid sequence has been identified in the C-terminal region of the 242-residue deduced fsrB product (J. Nakayama et al., Mol. Microbiol. 41:145-154, 2001). In this study, however, we demonstrated the existence of fsrD, encoding the GBAP propeptide, which is in frame with fsrB but is translated independently of fsrB. It was also demonstrated that FsrB', an FsrD segment-truncated FsrB, functions as a cysteine protease-like processing enzyme to generate GBAP from FsrD. This revised model is consistent with the staphylococcal agr system.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. nakayama@agr.kyushu-u.ac.jp
authors.forename: Jiro
authors.initials: J
authors.lastname: Nakayama
authors.forename: Shengmin
authors.initials: S
authors.lastname: Chen
authors.forename: Nozomi
authors.initials: N
authors.lastname: Oyama
authors.forename: Kenzo
authors.initials: K
authors.lastname: Nishiguchi
authors.forename: Essam A
authors.initials: EA
authors.lastname: Azab
authors.forename: Emi
authors.initials: E
authors.lastname: Tanaka
authors.forename: Reiko
authors.initials: R
authors.lastname: Kariyama
authors.forename: Kenji
authors.initials: K
authors.lastname: Sonomoto
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D003546:Cysteine Endopeptidases / Q000378:metabolism; D013293:Enterococcus faecalis / Q000502:physiology*; D007783:Lactones / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016366:Open Reading Frames / Q000235:genetics*; D010456:Peptides, Cyclic / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D053038:Quorum Sensing; D016415:Sequence Alignment
chemical_list: C414532:AgrD protein, Staphylococcus; D001426:Bacterial Proteins; D007783:Lactones; D010456:Peptides, Cyclic; C438382:gelatinase biosynthesis-activating pheromone; D003546:Cysteine Endopeptidases