title: Analytical strategies to investigate plant N-glycan profiles in the context of plant-made pharmaceuticals.
abstract: Plants are attractive hosts for the production of recombinant proteins. However, their inability to process authentic human N-glycan structures imposes a major limitation on their use as expression systems for therapeutic products. Several strategies have emerged to engineer plant N-glycans into human-compatible molecules. In this context, fast and reliable analytical strategies for the identification of plant N-glycan profiles have been developed to define the N-glycosylation pathways of crops, to monitor the production of plant-made pharmaceuticals and to assess in planta remodelling strategies.
authors.affiliation: CNRS-UMR 6037, GDR G3, IFRMP 23, Université de Rouen, 76821 Mont Saint Aignan Cédex, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Bardor
authors.forename: Gleysin
authors.initials: G
authors.lastname: Cabrera
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
authors.forename: Raymond A
authors.initials: RA
authors.lastname: Dwek
authors.forename: José A
authors.initials: JA
authors.lastname: Cremata
authors.forename: Patrice
authors.initials: P
authors.lastname: Lerouge
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002240:Carbohydrate Sequence; D006801:Humans; D008969:Molecular Sequence Data; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000378:metabolism*; D010944:Plants / Q000737:chemistry* / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D004364:Pharmaceutical Preparations; D011134:Polysaccharides