Genetic analysis of Thailand hantavirus in Bandicota indica trapped in Thailand.
Sixty one tissue samples from several rodent species trapped in five provinces of Thailand were examined for the presence of hantaviral markers by enzyme-immunoassay and immunoblotting. Four samples, all from the great bandicoot rat Bandicota indica, were confirmed positive for the hantaviral N-antigen. Two of them were trapped in Nakhon Pathom province, the other two in Nakhon Ratchasima province, approximately 250 km from the other trapping site. When analysed by RT-nested PCR, all four rodents were found positive for the hantaviral S- and M-segment nucleotide sequences. Genetic analysis revealed that the four newly described wild-type strains belong to Thailand hantavirus. On the phylogenetic trees they formed a well-supported cluster within the group of Murinae-associated hantaviruses and shared a recent common ancestor with Seoul virus.
Hugot
Jean-Pierre
JP
OSEB, UMR 5202 du CNRS, Muséum National d'Histoire naturelle, Paris, France. hugot@mnhn.fr
Plyusnina
Angelina
A
Herbreteau
Vincent
V
Nemirov
Kirill
K
Laakkonen
Juha
J
Lundkvist
Ake
A
Supputamongkol
Yupin
Y
Henttonen
Heikki
H
Plyusnin
Alexander
A
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D016679:Genome, Viral; D006476:Orthohantavirus / Q000235:genetics* / Q000302:isolation & purification; D015151:Immunoblotting / Q000379:methods; D008969:Molecular Sequence Data; D051189:Murinae / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013785:Thailand
D000956:Antigens, Viral