title: HIV-1 protease mutations and inhibitor modifications monitored on a series of complexes. Structural basis for the effect of the A71V mutation on the active site.
abstract: Two new X-ray structures of an HIV-1 protease mutant (A71V, V82T, I84V) in complex with inhibitors SE and SQ, pseudotetrapeptide inhibitors with an acyclic S-hydroxyethylamine isostere, were determined. Comparison of eight structures exploring the binding of four similar inhibitors--SE, SQ (S-hydroxyethylamine isostere), OE (ethyleneamine), and QF34 (hydroxyethylene)--to wild-type and A71V/V82T/I84V HIV-1 protease elucidates the principles of altered interaction with changing conditions. The A71V mutation, which is distant from the active site, causes changes in the structure of the enzyme detectable by the means of X-ray structure analysis, and a route of propagation of the effect toward the active site is proposed.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského nam. 2, 162 06 Praha 6, Czech Republic. skalova@imc.cas.cz
authors.forename: Tereza
authors.initials: T
authors.lastname: Skalova
authors.forename: Jan
authors.initials: J
authors.lastname: Dohnalek
authors.forename: Jarmila
authors.initials: J
authors.lastname: Duskova
authors.forename: Hana
authors.initials: H
authors.lastname: Petrokova
authors.forename: Martin
authors.initials: M
authors.lastname: Hradílek
authors.forename: Milan
authors.initials: M
authors.lastname: Soucek
authors.forename: Jan
authors.initials: J
authors.lastname: Konvalinka
authors.forename: Jindrich
authors.initials: J
authors.lastname: Hasek
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray; D004983:Ethanolamines / Q000737:chemistry*; D016333:HIV Protease / Q000737:chemistry* / Q000235:genetics*; D017320:HIV Protease Inhibitors / Q000737:chemistry*; D006860:Hydrogen Bonding; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D009154:Mutation; D009842:Oligopeptides / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D004983:Ethanolamines; D017320:HIV Protease Inhibitors; D008024:Ligands; D009842:Oligopeptides; D016333:HIV Protease