title: Genetic relatedness of six North-Indian butterfly species (Lepidoptera :Pieridae) based on 16S rRNA sequence analysis.
abstract: The present work involves the assessment of level of genetic relatedness or divergence amongst the six North-Indian species of Lepidoptera belonging to family Pieridae and sub family Pierinae on the basis of sequence variation of 16S ribosomal RNA. The PCR amplified products of these species were directly sequenced using ABI Prism BigDye Terminator Sequencing Kits (Applied Biosystems). The multiple nucleotide sequence alignment analysis has revealed several differences across these species. Significantly high percentage of A + T base composition content ranging between 73.13% (Ixias pyrene ) and 79.20 % (Pieris brassica) was observed in studied species. The percentage divergence in the investigated species of Pieridae family varied from 5.5% to 21.7%. The two species of Catopsilia revealed minimum sequence divergence of only 5.5%, whereas the other two groups of Ixias and Pieris revealed 15.5% and 8.6% sequence divergence, respectively. Pieris canidia and Ixias pyrene are genetically most divergent (21.7%) amongst the studied lepidopteran species. Phylogenetic analysis based on 16S rRNA nucleotide sequence revealed grouping of six species of Lepidoptera in the form of two different clusters, each cluster being represented by two species from the same genera. The separate taxonomic grouping of these Indian species has been observed when compared with several species of Piernae and Coliadinae subfamilies from other country isolates.
authors.affiliation: Department of Biotechnology, Panjab University, Chandigarh, 160014, India. resobti@pu.ac.in
authors.forename: Ranbir Chander
authors.initials: RC
authors.lastname: Sobti
authors.forename: Vijay Lakshmi
authors.initials: VL
authors.lastname: Sharma
authors.forename: Mamtesh
authors.initials: M
authors.lastname: Kumari
authors.forename: Tajinder Kaur
authors.initials: TK
authors.lastname: Gill
authors.forename: Jagmohan
authors.initials: J
authors.lastname: Singh
authors.forename: Monika
authors.initials: M
authors.lastname: Sodhi
authors.forename: Manishi
authors.initials: M
authors.lastname: Mukesh
authors.forename: Sugandha
authors.initials: S
authors.lastname: Bansal
authors.forename: Swati
authors.initials: S
authors.lastname: Arya
authors.forename: Savita
authors.initials: S
authors.lastname: Bisnoi
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002080:Butterflies / Q000235:genetics*; D016000:Cluster Analysis; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D012984:Software
chemical_list: D012336:RNA, Ribosomal, 16S