A novel matrix protein p10 from the nacre of pearl oyster (Pinctada fucata) and its effects on both CaCO3 crystal formation and mineralogenic cells.
A novel matrix protein, designated as p10 because of its apparent molecular mass of 10 kDa, was isolated from the nacreous layer of pearl oyster (Pinctada fucata) by reverse-phase high-performance liquid chromatography. In vitro crystallization experiments showed that p10 could accelerate the nucleation of calcium carbonate crystals and induce aragonite formation, suggesting that it might play a key role in nacre biomineralization. As nacre is known to contain osteogenic factors, two mineralogenic cell lines, MRC-5 fibroblasts and MC3T3-E1 preosteoblasts, were used to investigate the biological activity of p10. The results showed that p10 could increase alkaline phosphatase activity, an early marker of osteoblast differentiation, while the viability of MRC-5 and MC3T3-E1 remained unchanged after treatment of p10. Taken together, the findings led to identification of a novel matrix protein from the nacre of P. fucata that plays a role in both the mineral phase and in the differentiation of the cells involved in biomineralization.
Zhang
Cen
C
Institute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, People's Republic of China.
Li
Shuo
S
Ma
Zhuojun
Z
Xie
Liping
L
Zhang
Rongqing
R
D000818:Animals; D002119:Calcium Carbonate / Q000737:chemistry / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects; D049911:Pinctada / Q000378:metabolism*
D016326:Extracellular Matrix Proteins; D002119:Calcium Carbonate