Expression, purification and anti-tumor activity of curcin.
Curcin, purified from the seeds of Jatropha curcas, can be used as a cell-killing agent. Understanding the anti-tumor activity of the recombinant protein of curcin is important for its application in clinical medicine. The segment encoding the mature protein of curcin was inserted into Escherichia coli strain M15, and the recombinant strain was induced to express by isopropyl-beta-D-thiogalactopyranoside at a concentration of 0.5 mM. The recombinant protein was expressed in the form of inclusion bodies and purified by Ni-NTA affinity chromatography. The target protein was incubated with the tumor cells at different concentrations for different times and the results demonstrated that the target protein could inhibit the growth of tumor cells (NCL-H446, SGC-7901 and S180) at 5 microg/ml.
Luo
Meng-Jun
MJ
College of Life Sciences, Sichuan University, Chengdu 610041, China.
Yang
Xin-Yu
XY
Liu
Wei-Xin
WX
Xu
Ying
Y
Huang
Ping
P
Yan
Fang
F
Chen
Fang
F
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D031286:Jatropha / Q000235:genetics / Q000378:metabolism*; D009369:Neoplasms / Q000473:pathology*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry* / Q000235:genetics; D010940:Plant Proteins / Q000008:administration & dosage* / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D054789:Ribosome Inactivating Proteins, Type 1; D012639:Seeds / Q000235:genetics / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D010940:Plant Proteins; D011994:Recombinant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; C515388:curcin protein, Jatropha curcas