title: [Effects of Shengqing Capsules on expression of nuclear factor-kappaB protein in liver cells of guinea pigs with pigment gallstone].
abstract: OBJECTIVE
  To study the mechanisms of Shengqing Capsules, a compound Chinese herbal medicine for dispersing stagnated liver qi and promoting bile flow, in prevention and treatment of pigment gallstone.
  
  
  METHODS
  Liver cells from guinea pigs with pigment gallstone were primarily cultured in vitro. The serums containing different concentrations of Shengqing Capsules were prepared by serum pharmacological method. The primary cultured hepatocytes with excessive expression of nuclear factor-kappaB (NF-kappaB) were stimulated with lipopolysaccharides (LPS) and the effects of the drug-containing serums on the NF-kappaB expression were detected by Western-blotting.
  
  
  RESULTS
  Shengqing Capsules down-regulated the NF-kappaB expression increased by LPS stimulation. The effect is dose-dependent.
  
  
  CONCLUSIONS
  Shengqing Capsules can down-regulate the NF-kappaB expression increased by LPS stimulation. It may be one of the important mechanisms of this Chinese herbal medicine in prevention and treatment for pigment gallstone formation and the inflammatory reaction in biliary tract.
authors.affiliation: Department of Hepatobiliary Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Gu
authors.forename: Jing-Zhe
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Xue-Lin
authors.initials: XL
authors.lastname: Zhang
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Liu
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Pei-Ting
authors.initials: PT
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001654:Bile Pigments / Q000378:metabolism; D015153:Blotting, Western; D002214:Capsules; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D042882:Gallstones / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D006168:Guinea Pigs; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D016328:NF-kappa B / Q000096:biosynthesis*; D044967:Serum
chemical_list: D001654:Bile Pigments; D002214:Capsules; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D016328:NF-kappa B; C527180:shengqing