Application of the EMIT 2000 Tacrolimus assay on the Abbott Architect c8000 high volume clinical chemistry analyzer.
OBJECTIVE
Evaluation of the performance of the EMIT 2000 Tacrolimus assay on the Abbott Architect c8000 analyzer.


METHODS
Imprecision studies were performed and patient samples were assayed by EMIT assay and by LC-MS/MS.


RESULTS
Limit of quantification was established at 2.8 microg/L. A positive bias of 17.5% between results measured on EMIT and on LC-MS/MS was detected.


CONCLUSIONS
EMIT 2000 Tacrolimus assay is suitable for automated analyses of Tacrolimus on the Architect c8000.
Boer
Klas
K
Institut fur Klinische Chemie und Labordiagnostik FSU Jena, Germany. kboeer@med.uni-jena.de
Deufel
Thomas
T
Schmidt
Dirk
D
Streck
Sibylle
S
Kiehntopf
Michael
M
D002853:Chromatography, Liquid; D019963:Clinical Chemistry Tests / Q000295:instrumentation*; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood*; D013058:Mass Spectrometry; D012015:Reference Standards; D015203:Reproducibility of Results; D016559:Tacrolimus / Q000097:blood*
D007166:Immunosuppressive Agents; D016559:Tacrolimus