Effects of insufficient air injection on methanogenic Archaea in landfill bioreactor.
In this study, methanogenic Archaea diversity in an aerated landfill bioreactor filled with co-disposed incineration bottom ashes and shredded incombustible wastes was monitored and analyzed as a function of time using molecular techniques. Besides, the effects of insufficient air injection on the bioreactor performance and methanogenic diversity were evaluated thoroughly. Results indicated that rapid bio-stabilization of solid waste are possible with aerated landfill bioreactor at various oxygen and oxidation reduction potential levels. Slot-blot hybridization results of leachate samples collected from aerated landfill bioreactor showed that archaeal and bacterial activities increased as stabilization accelerated and bacterial populations constituted almost 95% of all microorganisms. The results of slot-blot hybridization and phylogenetic analysis based on 16S rRNA gene revealed that Methanobacteriales and Methanomicrobiales were dominant species at the beginning while substituted by Methanosarcina-related methanogens close to the end of the operation of bioreactor.
Mertoglu
Bulent
B
Department of Environmental Engineering, Marmara University, 34722 Goztepe, Istanbul, Turkey. bmertoglu@eng.marmara.edu.tr
Calli
Baris
B
Guler
Nuray
N
Inanc
Bulent
B
Inoue
Yuzo
Y
D000388:Air; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D019149:Bioreactors; D015169:Colony Count, Microbial; D017931:DNA Primers; D008697:Methane / Q000096:biosynthesis*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D008697:Methane