Cloning and sequence analysis of the N gene of porcine epidemic diarrhea virus LJB/03.
The nucleocapsid (N) gene of the porcine epidemic diarrhea virus (PEDV) strain LJB/03 which was previously isolated in Heilongjiang province, China, was cloned, sequenced and compared with published sequences of other avian and mammalian coronavirus. The nucleotide sequence encoding the entire N gene open reading frame (ORF) of LJB/03 was 1326 bases long and encoded a protein of 441 amino acids with predicted Mr of 49 kDa. It consisted of 405 adenines (30.5%), 294 cytosines (22.1%), 329 guanines (24.8%) and 298 thymines (22.5%) residues. Sequence comparison with other PEDV strains selected from GenBank revealed that the LJB/03 N gene has a high sequence homology to those of other PEDV isolates, 97.4% with JS2004, 95.6% with chinju99, 96.6% with Br1/87, and 96.8% with CV777. The encoded protein shared 96.4% amino acid identities compared with CV777, 96.1% with Brl/87, 98% with JS2004, 96.90% with chinju99, respectively. The amino acid sequence contained seven potential protein kinase C phosphorylation sites, nine Casein kinase II phosphorylation sites, one Tyrosine kinase phosphorylation site, two cAMP- and cGMP-dependent protein kinase phosphorylation sites.
Junwei
Ge
G
Veterinary Department, Northeast Agriculture University, Harbin 150030, PR China.
Baoxian
Li
L
Lijie
Tang
T
Yijing
Li
L
D000818:Animals; D003001:Cloning, Molecular; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D000086462:Coronavirus Nucleocapsid Proteins; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D005243:Feces / Q000821:virology; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000235:genetics*; D010802:Phylogeny; D053485:Porcine epidemic diarrhea virus / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000821:virology*
D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins