Comparison of changes in circulating platelet-derived microparticles and platelet surface P-selectin expression after coronary stent implantation.
Platelet-derived microparticles (PDMPs) are released from activated platelets and may participate in the inflammatory process in response to vessel wall injury. This study was designed to compare the clinical significance of circulating PDMPs with that of P-selectin on the platelet membrane surface. In 20 patients with stable angina undergoing coronary stent implantation, circulating PDMPs were serially measured by enzyme-linked immunosorbent assay, and P-selectin expression on the surface of platelets was simultaneously analyzed by flow cytometry. PDMPs increased 24-48 h after coronary stenting in the coronary sinus (8.7 +/- 8.9 to 31.8 +/- 19.8 U/ml, P < 0.001) with a maximum at 48 h. In contrast, the mean channel fluorescence intensity for P-selectin increased 15 min after coronary stenting in the coronary sinus (19.5 +/- 5.6 to 25.2 +/- 7.5, P < 0.01) and remained elevated for 48 h; the changes were less striking in peripheral blood. The relative increase in PDMPs was not correlated with the increase in P-selectin expression at 15 min or 24 h after coronary stenting, but was correlated at 48 h (R = 0.48, P < 0.05). Both circulating PDMPs and P-selectin expression were enhanced in association with stent-induced platelet activation; however, the time course of changes in these two platelet activation markers was different. Therefore, the clinical relevance of circulating PDMPs may differ from that of P-selectin expression on the platelet membrane surface.
Inoue
Teruo
T
Faculty of Medicine, Department of Cardiovascular and Renal Medicine, Saga University, Saga, Japan. inouete@med.saga-u.ac.jp
Hikichi
Yutaka
Y
Morooka
Toshihumi
T
Yoshida
Kazuyo
K
Fujimatsu
Daisuke
D
Komoda
Hiroshi
H
Kameda
Miho
M
Nonaka
Masako
M
Sohma
Ryoichi
R
Hashimoto
Shigemasa
S
Node
Koichi
K
D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D000789:Angina, Unstable / Q000628:therapy; D001792:Blood Platelets / Q000378:metabolism*; D002462:Cell Membrane / Q000502:physiology*; D003331:Coronary Vessels / Q000502:physiology; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000378:metabolism*; D015539:Platelet Activation / Q000502:physiology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D015607:Stents; D013988:Ticlopidine / Q000494:pharmacology
D019007:P-Selectin; D010975:Platelet Aggregation Inhibitors; D013988:Ticlopidine