Effect of inhibitor of tumor necrosis factor-alpha and oxatomide on immune mediated otitis media.
OBJECTIVE
Inflammatory mediators (IMs) play a major role in the production of middle ear effusion (MEE). Tumor necrosis factor (TNF)-alpha and leukotrienes (LTs) appear to be important in the pathogenesis of otitis media with effusion (OME). The purpose of this study is to determine the effect of TNF-alpha and LT antagonist on the outcome of experimental immune-mediated OME.


METHODS
Prospective.


METHODS
Otitis media was induced in rats by injecting keyhole limpet hemocyanin (KLH) transtympanically 7 days after systemic immunization. Experimental groups were treated with soluble TNF receptor type I (sTNF RI) or oxatomide simultaneously. Seventy-two hours after transtympanic injection, MEE was aspirated, and temporal bone was taken. Vascular permeability (VP) of the middle ear mucosa was measured using the Evans blue dye technique. Hematoxylin-eosin stain and immunohistochemical stain for leukocyte common antigen was performed.


RESULTS
In KLH, sTNF RI, and oxatomide groups, MEE was developed in 83%, 0%, and 66% of the ears, respectively. The sTNF RI group showed significant decrease in effusion production, inflammation, mucosal thickening, and VP compared with the KLH group. These parameters were less significant in the oxatomide group than in the sTNF RI group.


CONCLUSIONS
Transtympanic administration of sTNF RI and oxatomide appears to suppress the development of immune-mediated MEE.
Park
Yong-Soo
YS
Department of Otolaryngology-HNS, College of Medicine, The Catholic University of Korea, Incheon, Korea.
Auo
Hyeon-Jin
HJ
Jeon
Eun-Ju
EJ
Chang
Ki-Hong
KH
Lee
Seung Kyun
SK
Lee
Dong-Hee
DH
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D004432:Ear, Middle / Q000098:blood supply / Q000473:pathology; D005070:Evans Blue; D006433:Hemocyanins; D007124:Immunoenzyme Techniques; D010034:Otitis Media with Effusion / Q000188:drug therapy*; D010784:Photomicrography; D010879:Piperazines / Q000494:pharmacology*; D011446:Prospective Studies; D051381:Rats; D017207:Rats, Sprague-Dawley; D018124:Receptors, Tumor Necrosis Factor / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
D010879:Piperazines; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D005070:Evans Blue; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; C015408:oxatomide