title: Phenylalanine dehydrogenase mutants: efficient biocatalysts for synthesis of non-natural phenylalanine derivatives.
abstract: Wild-type phenylalanine dehydrogenase from Bacillus sphaericus, and three mutants N145A, N145V and N145L, are used with a coenzyme recycling system to synthesise L-phenylalanine and three non-natural amino acids (p-F-phenylalanine, p-MeO-phenylalanine and p-CF(3)-phenylalanine) on a millimole scale. A range of reaction conditions are investigated. The kinetic parameters of WT PheDH and N145A towards p-CF(3)-phenylpyruvate are compared, emphasising the value of protein engineering in creating improved biocatalysts.
authors.affiliation: UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Francesca
authors.initials: F
authors.lastname: Paradisi
authors.forename: Stuart
authors.initials: S
authors.lastname: Collins
authors.forename: Anita R
authors.initials: AR
authors.lastname: Maguire
authors.forename: Paul C
authors.initials: PC
authors.lastname: Engel
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D001711:Biotransformation; D007700:Kinetics; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D010649:Phenylalanine / Q000096:biosynthesis*; D013237:Stereoisomerism
chemical_list: D010649:Phenylalanine; D000594:Amino Acid Oxidoreductases; C022342:phenylalanine oxidase