Technical note: assay of cell quantity in the fibroblast-populated collagen matrix with a tetrazolium reagent.
Determination of cell quantity in three-dimensional culture systems, such as the fibroblast-populated collagen matrix, can be difficult secondary to the abundance of extracellular collagen. The WST-1 assay, which can quantitate cellular processes in monolayer culture, was tested as a measure of relative cell quantity in a three dimensional culture system, the fibroblast-populated collagen matrix. The assay had acceptable accuracy and precision, and was not affected by the extracellular matrix. It was concluded that the assay was a reasonable alternative to other measures of cell quantity in the fibroblast-populated collagen matrix.
Carlson
Mark A
MA
Department of Surgery, University of Nebraska Medical Center and the Omaha VA Medical Center, Omaha, NE 68105, USA. macarlso@unmc.edu
D002452:Cell Count / Q000379:methods*; D018929:Cell Culture Techniques; D003094:Collagen; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007202:Indicators and Reagents / Q000032:analysis; D013778:Tetrazolium Salts / Q000032:analysis*
C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; D003094:Collagen