Transgenesis using nuclear transfer in goats.
Nuclear transfer (NT) using transgenic donor cells is an efficient means for generation of transgenic founder goats, especially in regard to the number of animals required to produce a transgenic founder expressing the protein of interest. Vectors can be designed for organ-specific expression and secretion of recombinant proteins within the target tissue. Furthermore, donor cells can be selected for gender, genetically modified to introduce the transgene of interest and screened for incorporation of the transgene into the genome before use in NT. This chapter describes methods for production of transgenic donor cells, subsequent NT embryo production, and transfer into recipients.
Lazaris
Anthoula
A
Quebec Transgenic Research Network, McGill University, Montreal, Quebec, Canada.
Keyston
Rebecca
R
Karatzas
Costas N
CN
Keefer
Carol L
CL
D000818:Animals; D030801:Animals, Genetically Modified; D018929:Cell Culture Techniques / Q000662:veterinary; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000662:veterinary*; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D005333:Fetus / Q000166:cytology; D005347:Fibroblasts; D018014:Gene Transfer Techniques; D006041:Goats / Q000235:genetics*; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D014019:Tissue Donors; D014162:Transfection