Experimental model in the qualitative and quantitative assessment of non-Helicobacter gastric microflora under proton pump inhibitors action.
OBJECTIVE
To evaluate models of gastric material collection from Wistar rats with and without using proton pump inhibitors(PPIs).


METHODS
Twenty-four rats underwent intraperitoneal omeprazol treatment, and other 12 received similar treatment with 0.9% saline. All animals underwent collection of gastric material samples, after stomach removal, by either biopsies, or aspirates, or swabs. Samples were bacteriologically processed in order to identify species and strains. Values are described as natural logarithm of colony former units per mL [Ln(CFU/mL)]. Kruskal-Wallis and Mann-Whitney non-parametric tests were used, and p<0.05 was set as statistically significant.


RESULTS
Significant difference was not seen for Ln (UFC/mL) values among the three methods of collection irrespective of using or not omeprazol. Also, significant difference was not seen in Ln (UFC/mL) values when comparing a method with each others, either using omeprazol or placebo. A significant increase of bacteria strains occurred when PPI was used, and this was seen on the three ways of collection, mainly in biopsy and swab.


CONCLUSIONS
No difference occurred among the three methods of collecting bacteria samples from stomachs of rats, either when using placebo or omeprazol. A remarkable change is seen on animals bacterial microflora when PPIs are used, and bacteria are better identified when swab and biopsy are used.
Diogo Filho
Augusto
A
Experimental Laboratory, Division of Surgical Techniques, Surgery Department, Federal University-Uberlândia Medical School (FAMED-UFU), Av. Levino de Souza 1775, 38405-322 Uberlândia, Minas Gerais, Brazil. diogofilho@netsite.com.br
Santos
Pablo Silva
PS
Duque
Anderson Silveira
AS
Cezário
Renata Cristina
RC
Gontijo Filho
Paulo Pinto
PP
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D001707:Biopsy, Needle; D015169:Colony Count, Microbial; D005753:Gastric Mucosa / Q000382:microbiology*; D008297:Male; D009853:Omeprazole / Q000494:pharmacology; D054328:Proton Pump Inhibitors; D017494:Proton Pumps / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013048:Specimen Handling / Q000379:methods* / Q000592:standards; D018709:Statistics, Nonparametric
D000897:Anti-Ulcer Agents; D054328:Proton Pump Inhibitors; D017494:Proton Pumps; D009853:Omeprazole