Analysis of 5-hydroxymethylfurfural in foods by gas chromatography-mass spectrometry.
A new, simple and selective method for the analysis of 5-hydroxymethylfurfural (HMF) in foods by gas chromatography coupled to mass spectrometry (GC-MS) is proposed. Several derivatising procedures based on the formation of an HMF silylated derivative using different reagents were studied. Among the derivatising reagents examined, N,O-bis-trimethylsilyltrifluoroacetamide (BSTFA) provided the best derivatisation yield. Sample clean-up was also optimised, using either liquid-liquid extraction with dichloromethane or solid-phase extraction (SPE) with several commercially available cartridges, and the best results were obtained using ENV+ cartridges. Quality parameters such as day-to-day and run-to-run precision (RSD<10%), linearity (between 25 and 700 ng g(-1)) and detection limit (6 ng g(-1)) were established. This method was successfully applied to the analysis of HMF content in several Spanish food samples from a local market, such as jam, honey, orange juice and bakery products.
Teixidó
E
E
Departament de Química Analítica, Facultat de Química, Universitat de Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain.
Santos
F J
FJ
Puignou
L
L
Galceran
M T
MT
D000081:Acetamides; D005463:Fluoroacetates; D005504:Food Analysis / Q000379:methods*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013030:Spain; D013997:Time Factors; D014269:Trifluoroacetic Acid / Q000737:chemistry; D014297:Trimethylsilyl Compounds / Q000737:chemistry*
D000081:Acetamides; D005463:Fluoroacetates; D014297:Trimethylsilyl Compounds; C086665:N-methyl-N-(trimethylsilyl)trifluoroacetamide; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde; D014269:Trifluoroacetic Acid