title: Positively coded photoaffinity label for altering isoelectric points of proteins.
abstract: Novel diazirinyl photoaffinity ligand, which contains (3-trifluoromethyl) phenyldiazirine and penta(epsilon-Boc-Lys) as a photoreactive code, allows the introduction of a positive cascade to alter the pI value of labeled components, facilitating the isolation of photolabeled biocomponents with isoelectric focusing techniques.
authors.affiliation: Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. hasimoto@obihiro.ac.jp <hasimoto@obihiro.ac.jp>
authors.forename: Makoto
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Yasumaru
authors.initials: Y
authors.lastname: Hatanaka
mesh_terms: D007526:Isoelectric Point; D049449:Luminescence; D015394:Molecular Structure; D019920:Photoaffinity Labels / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D019920:Photoaffinity Labels; D011506:Proteins