Preparation of monoclonal antibody against celangulin V and immunolocalization of receptor in the oriental armyworm, Mythimna separata walker (Lepidoptera: Noctuidae).
The botanical insecticide celangulin V (CA-V) is an insect digestive poison acting on midgut tissue of the target insect larvae. With the aim of localizing the receptor enacted by CA-V, monoclonal antibodies (MAbs) specific to the compound were developed. A hapten was synthesized by introducing a succinoyl into the CA-V structure and conjugated with three carrier proteins. From mice immunized with one conjugate, three MAbs were obtained with a potential capacity of detecting protein-bound residue forms of CA-V in the biological tissues. The oriental armyworm larvae ingested CA-V were examined by the technique of immuno-electron-microscopy (IEM) using the anti-CA-V MAb as the primary antibody and goat anti-mouse/IgG labeled with colloidal gold as the secondary antibody. Electron micrographs of the armyworm midgut tissues showed that the CA-V was associated with the midgut epithelia of the insects. These results demonstrated the existence of a receptor enacted by CA-V on the midgut cells of the oriental armyworm larvae.
Qi
Zhijun
Z
Institute of Pesticide Science, Northwest Agriculture and Forestry University, Yangling 712100, China.
Xue
Xiaoping
X
Wu
Wenjun
W
Zhang
Jiwen
J
Yang
Runya
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D007306:Insecticides / Q000276:immunology*; D007915:Lepidoptera / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D011955:Receptors, Drug / Q000032:analysis*
D000911:Antibodies, Monoclonal; D006241:Haptens; D007306:Insecticides; D011955:Receptors, Drug; C514792:celangulin V