Monitoring DPA release from a single germinating Bacillus subtilis endospore via surface-enhanced Raman scattering microscopy.
A method for monitoring DPA release from a single germinating Bacillus subtilis endospore is reported. High S/N ratio SERS spectra were obtained with excitation power 3 mW at 647.1 nm and 1 min spectral collection times. The method is proof-of-principle for the SERS detection limit at the single spore level. This represents a 100- to 1000-fold improvement over previously reported detection limits for SERS-based measurements of DPA in endospores.
Evanoff
David D
DD
Department of Chemistry, Clemson University, Clemson, SC 29634, USA.
Heckel
John
J
Caldwell
Thomas P
TP
Christensen
Kenneth A
KA
Chumanov
George
G
D001412:Bacillus subtilis / Q000378:metabolism / Q000502:physiology*; D008853:Microscopy / Q000379:methods; D010848:Picolinic Acids / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013171:Spores, Bacterial
D010848:Picolinic Acids; C004860:dipicolinic acid