Magnetic resonance imaging of gadolinium-labeled pancreatic islets for experimental transplantation.
New imaging techniques that couple anatomical resolution to sensitivity may greatly contribute to improving islet transplantation. In the present work, a report is given of the direct detection of islets by magnetic resonance imaging (MRI) after ex vivo cell labeling with the MRI T(1) contrast agent GdHPDO3A. Experiments on mouse and human islets demonstrated well-tolerated uptake of GdHPDO3A, based on morphology, viability, glucose-dependent insulin response and apoptosis/toxicity gene array profile. GdHPDO3A loading was sufficient for in vitro MRI cell detection. In vivo isotransplanted mouse islets into the kidney capsule and xenotransplanted human islets within the mouse liver were detected. Imaging specificity was supported by the absence of signal in unlabeled islet transplants, its persistence upon using fat-suppression MRI protocols and the colocalization with the transplanted islets. In conclusion, direct islet imaging with high spatial and contrast resolution after labeling with GdHPDO3A is demonstrated, allowing visualization of kidney subcapsular mouse islet grafts and intrahepatic human islet xenografts.
Biancone
Luigi
L
Department of Internal Medicine and Research Center for Experimental Medicine (CeRMS), University of Turin, Corso Dogliotti 14, Turin 10126, Italy.
Crich
Simonetta Geninatti
SG
Cantaluppi
Vincenzo
V
Romanazzi
Giuseppe Mauriello
GM
Russo
Simona
S
Scalabrino
Elisa
E
Esposito
Giovanna
G
Figliolini
Federico
F
Beltramo
Silvia
S
Perin
Paolo Cavallo
PC
Segoloni
Giuseppe Paolo
GP
Aime
Silvio
S
Camussi
Giovanni
G
D000818:Animals; D003287:Contrast Media / Q000032:analysis* / Q000494:pharmacology; D005682:Gadolinium; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006571:Heterocyclic Compounds; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D007668:Kidney; D008099:Liver; D008279:Magnetic Resonance Imaging; D013058:Mass Spectrometry; D051379:Mice; D016513:Mice, SCID; D020411:Oligonucleotide Array Sequence Analysis; D009942:Organometallic Compounds / Q000032:analysis* / Q000494:pharmacology; D011169:Portal Vein; D014183:Transplantation, Heterologous; D016042:Transplantation, Heterotopic; D014185:Transplantation, Isogeneic
D003287:Contrast Media; D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; C062402:gadoteridol; D005682:Gadolinium