title: Wall teichoic acid polymers are dispensable for cell viability in Bacillus subtilis.
abstract: An extensive literature has established that the synthesis of wall teichoic acid in Bacillus subtilis is essential for cell viability. Paradoxically, we have recently shown that wall teichoic acid biogenesis is dispensable in Staphylococcus aureus (M. A. D'Elia, M. P. Pereira, Y. S. Chung, W. Zhao, A. Chau, T. J. Kenney, M. C. Sulavik, T. A. Black, and E. D. Brown, J. Bacteriol. 188:4183-4189, 2006). A complex pattern of teichoic acid gene dispensability was seen in S. aureus where the first gene (tarO) was dispensable and later acting genes showed an indispensable phenotype. Here we show, for the first time, that wall teichoic acid synthesis is also dispensable in B. subtilis and that a similar gene dispensability pattern is seen where later acting enzymes display an essential phenotype, while the gene tagO, whose product catalyzes the first step in the pathway, could be deleted to yield viable mutants devoid of teichoic acid in the cell wall.
authors.affiliation: Antimicrobial Research Centre and Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, L8N 3Z5, Canada.
authors.forename: Michael A
authors.initials: MA
authors.lastname: D'Elia
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Millar
authors.forename: Terry J
authors.initials: TJ
authors.lastname: Beveridge
authors.forename: Eric D
authors.initials: ED
authors.lastname: Brown
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D002473:Cell Wall / Q000737:chemistry*; D017353:Gene Deletion; D005798:Genes, Bacterial; D011108:Polymers / Q000378:metabolism*; D013682:Teichoic Acids / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011108:Polymers; D013682:Teichoic Acids