title: Transverse relaxation in rat optic nerve.
abstract: In vitro investigations were performed to study the proton T(2) relaxation spectrum of rat optic nerve. Studies in which the nerve was incubated in a D(2)O-based solution revealed that >98% of the spectrum originated from the water protons. The spectrum was found to consist of three components having relaxation times and sizes similar to those reported in the literature for peripheral nerve. Procedures were taken to confirm the existence of a third optic nerve component and that it was not an artifact of the long-lived water protons of the in vitro incubation solution. Evidence using paramagnetic agents in the incubation solution, which removed the two longest-lived nerve components from the spectrum, revealed the existence of a small fourth component (<10% of total) having a T(2) relaxation time similar to that of the intermediate-lived nerve component. Bathing the nerves in a 10 mM glutamate solution, glutamate known to result in cellular swelling in mammalian central nervous system (CNS), was found to increase the component size of the longest-lived nerve component, suggestive that this component may result from cellular water.
authors.affiliation: Department of Biomedical Engineering, University of Alberta, Edmonton, Alberta T6G 2V2, Canada.
authors.forename: Isidro
authors.initials: I
authors.lastname: Bonilla
authors.forename: Richard E
authors.initials: RE
authors.lastname: Snyder
mesh_terms: D000818:Animals; D018698:Glutamic Acid / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008345:Manganese / Q000494:pharmacology; D009900:Optic Nerve / Q000166:cytology*; D026902:Potassium Channel Blockers / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D026941:Sodium Channel Blockers / Q000494:pharmacology
chemical_list: D026902:Potassium Channel Blockers; D026941:Sodium Channel Blockers; D018698:Glutamic Acid; D008345:Manganese