Chemically selective sensing through layer-by-layer incorporation of biorecognition into thin film substrates for surface-enhanced resonance Raman scattering.
In this work, the fabrication, characterization, and application of avidin/Ag nanoparticle layer-by-layer (LbL) films as chemically selective substrates for surface-enhanced resonance Raman scattering (SERRS) is demonstrated. The biospecific interaction between avidin and the small molecule biotin, one of the strongest known to exist in nature, is exploited to preferentially capture biotinylated species from solution. This highly favored adsorption is shown to yield SERRS concentration enhancements and improved detection sensitivities of ca. 102 for commercially available and in situ prepared biotinylated species over their nontagged counterparts.
Pieczonka
Nicholas P W
NP
Materials and Surface Science Group, Department of Chemistry and Biochemistry, University of Windsor, Windsor, Ontario, Canada, N9B 3P4.
Goulet
Paul J G
PJ
Aroca
Ricardo F
RF
D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods
D003102:Colloids; D001360:Avidin; D012834:Silver; D001710:Biotin