Interaction of tin(II) and arsenic(III) with DNA at the nanostructure film modified electrodes.
Biosensors based on DNA and DNA-carbon nanotubes film immobilized at the surface of a screen-printed carbon electrode were used for simple in vitro tests of chemical toxicity. The damage to DNA caused by tin(II) and arsenic(III) compounds as components of specific reaction media was evaluated by means of an electrochemical DNA marker, [Co(phen)3](3+), as the portion of original dsDNA which survives an incubation of the biosensor in the cleavage medium. The results were confirmed by the electrically heated electrode and by the measurement of the DNA guanine moiety signal.
Ferancová
Adriana
A
Department of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, FCHPT STU, Radlinského 9, 81237 Bratislava, Slovakia. adriana.ferancova@stuba.sk
Adamovski
Miriam
M
Gründler
Peter
P
Zima
Jirí
J
Barek
Jirí
J
Mattusch
Jürgen
J
Wennrich
Rainer
R
Labuda
Ján
J
D001151:Arsenic / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002412:Cations; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry; D013696:Temperature; D014001:Tin / Q000737:chemistry*; D018675:Toxicity Tests / Q000295:instrumentation / Q000379:methods*
D002412:Cations; D037742:Nanotubes, Carbon; D009942:Organometallic Compounds; D010618:Phenanthrolines; C414956:triphenanthrolinecobalt(II); D006147:Guanine; D014001:Tin; D004247:DNA; D001151:Arsenic