title: Efficient reactivation of latent herpes simplex virus from mouse central nervous system tissues.
abstract: For decades, numerous ex vivo studies have documented that latent herpes simplex virus (HSV) reactivates efficiently from ganglia, but rarely from the central nervous systems (CNS), of mice when assayed by mincing tissues before explant culture, despite the presence of viral genomes in both sites. Here we show that 88% of mouse brain stems reactivated latent virus when they were dissociated into cell suspensions before ex vivo explant culture. The efficient reactivation of HSV from the mouse CNS was demonstrated with more than one viral strain, viral serotype, and mouse strain, further indicating that the CNS can be an authentic latency site for HSV with the potential to cause recurrent disease.
authors.affiliation: Department of Microbiology and Immunology, Medical College, National Cheng Kung University, Tainan, Taiwan 701, Republic of China.
authors.forename: Shih-Heng
authors.initials: SH
authors.lastname: Chen
authors.forename: Hui-Wen
authors.initials: HW
authors.lastname: Yao
authors.forename: Wen-Yen
authors.initials: WY
authors.lastname: Huang
authors.forename: Kuei-Sen
authors.initials: KS
authors.lastname: Hsu
authors.forename: Huan-Yao
authors.initials: HY
authors.lastname: Lei
authors.forename: Ai-Li
authors.initials: AL
authors.lastname: Shiau
authors.forename: Shun-Hua
authors.initials: SH
authors.lastname: Chen
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002490:Central Nervous System / Q000502:physiology / Q000821:virology*; D051379:Mice; D018139:Simplexvirus / Q000502:physiology*; D014775:Virus Activation / Q000502:physiology*; D017735:Virus Latency / Q000502:physiology*