title: Identification of a 2-stage platelet aggregation process mediating shear-dependent thrombus formation.
abstract: Disturbances of blood flow at sites of atherosclerotic plaque rupture are one of the key pathogenic events promoting platelet activation and arterial thrombus formation. Shear effects of platelets have been extensively investigated in vitro; however, the mechanisms by which shear promotes platelet aggregation in vivo remain incompletely understood. By employing high-resolution imaging techniques to in vitro and in vivo thrombosis models, we demonstrate a unique mechanism initiating shear-dependent platelet aggregation involving aggregate formation between discoid platelets. These discoid platelet aggregates are initially unstable and result from the development of membrane tethers between coadhering platelets. Tether formation involves the adhesive function of GPIb/V/IX and integrin alphaIIbbeta3, and conversion of discoid platelet aggregates into stable aggregates requires released ADP. The efficiency of this process is regulated by 3 independent variables, including the reactivity of the adhesive substrate, the level of shear flow, and the platelet density at the adhesive surface. These studies identify a new mechanism initiating platelet aggregation that is critically influenced by shear, physical proximity between translocating platelets, and membrane tether formation. Moreover, they provide a model to explain how the discoid morphology of platelets facilitates the maintenance of adhesive interactions with thrombogenic surfaces under high shear stress conditions.
authors.affiliation: Australian Centre for Blood Diseases, Monash University, 89 Commercial Rd, Prahran, Australia, 3181.
authors.forename: Mhairi J
authors.initials: MJ
authors.lastname: Maxwell
authors.forename: Erik
authors.initials: E
authors.lastname: Westein
authors.forename: Warwick S
authors.initials: WS
authors.lastname: Nesbitt
authors.forename: Simon
authors.initials: S
authors.lastname: Giuliano
authors.forename: Sacha M
authors.initials: SM
authors.lastname: Dopheide
authors.forename: Shaun P
authors.initials: SP
authors.lastname: Jackson
mesh_terms: D000244:Adenosine Diphosphate / Q000502:physiology; D000818:Animals; D001792:Blood Platelets / Q000502:physiology / Q000648:ultrastructure; D002452:Cell Count; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D018056:Hemorheology / Q000379:methods; D006801:Humans; D008297:Male; D010974:Platelet Aggregation / Q000502:physiology*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D013499:Surface Properties; D013927:Thrombosis / Q000097:blood / Q000378:metabolism*
chemical_list: D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D000244:Adenosine Diphosphate