Effects of hydroxysterols and atorvastatin on lipopolysaccharide-induced secretion of tumor necrosis factor and interleukin-10 by mouse macrophages.
Preincubation of macrophages with atorvastatin, cholesterol, 25-, 27-hydroxycholesterol, and 7-ketocholesterol reduced the level of TNF-alpha to 10, 61, 13, 64.5, and 82%, respectively. Addition of mevalonate to the preincubation medium canceled the effects atorvastatin, cholesterol, and 7-ketocholesterol, but not the effects of 25- and 27-hydroxycholesterols. Atorvastatin increased the level of IL-10 by 41%, while 7-ketocholesterol and 25-hydroxycholesterol inhibited its secretion by 48 and 55%.
Dushkin
M I
MI
Institute of Therapy, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk. midushkin@soramn.ru
Khoshchenko
O M
OM
Kudinova
E N
EN
Schwartz
Ya Sh
YSh
D000818:Animals; D000069059:Atorvastatin; D002784:Cholesterol / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D006538:Heptanoic Acids / Q000494:pharmacology*; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011758:Pyrroles / Q000494:pharmacology*; D013261:Sterols / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D006538:Heptanoic Acids; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D011758:Pyrroles; D013261:Sterols; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D002784:Cholesterol; D000069059:Atorvastatin