Neuropeptide Y (NPY) modulates oxidative burst and nitric oxide production in carrageenan-elicited granulocytes from rat air pouch.
We studied the effects of neuropeptide Y (NPY) and NPY-related receptor specific peptides on functions of carrageenan-elicited granulocytes in vitro and ability of NPY to modulate carrageenan-induced air pouch inflammation in rats in vivo. Anti-inflammatory effect of NPY comprises reduced granulocyte accumulation into the air pouch, to some extent attenuation of phagocytosis, attained via Y1 receptor, and considerable decrease in peroxide production, albeit mediated via Y2 and Y5 receptors activation. Conversely, NPY increases nitric oxide production and this potentiation is mediated via Y1 receptor. It is concluded that NPY Y1 and Y2/Y5 receptors' interaction participates in NPY-induced modulation of granulocyte functions related to inflammation.
Dimitrijević
Mirjana
M
Immunology Research Center "Branislav Janković", Institute of Immunology and Virology "Torlak", Vojvode Stepe 458, 11152 Belgrade, Serbia. ilijadim@sezampro.yu
Stanojević
Stanislava
S
Mićić
Stana
S
Vujić
Vesna
V
Kovacević-Jovanović
Vesna
V
Mitić
Katarina
K
von Hörsten
Stephan
S
Kosec
Dusko
D
D000818:Animals; D002351:Carrageenan / Q000008:administration & dosage*; D006098:Granulocytes / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D018836:Inflammation Mediators / Q000502:physiology*; D009478:Neuropeptide Y / Q000502:physiology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D051381:Rats; D016897:Respiratory Burst / Q000502:physiology*; D012867:Skin / Q000378:metabolism / Q000473:pathology
D018836:Inflammation Mediators; D009478:Neuropeptide Y; D009569:Nitric Oxide; D002351:Carrageenan