title: [Analysis on the genetic characteristics of the wide-type measles virus circulating in Zhejiang province in 2005].
abstract: OBJECTIVE
  To study the genetic characteristics of measles viruses circulating in Zhejiang province in 2005.
  
  
  METHODS
  4 groups of measles viruses isolated in outbreaks and the H and N gene were amplified by reverse transcription polymerase chain reaction (RT-PCR). PCR products were purified, sequenced and data was analyzed.
  
  
  RESULTS
  All of the 4 measles isolates belonged to genotype H1 which had been a main genotype containing all of the isolates in China. The isolates shared 99.2% -99.7% identity of amino acid sequence on H and 99.8% identity of amino acid sequence on N gene. When comparing to the China vaccine strain (Shanghai 191), there were 95.2%-95.5% homogeneties and 95.5% homogeneties on H and N gene respectively.
  
  
  CONCLUSIONS
  Data from phylogenic trees of H and N gene revealed that the wide-type measles viruses circulating in Zhejiang province in 2005 all belonged to genotype H1. There were obvious differences on genetic characteristics between the isolates and the genotype A (Shanghai 191).
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310009, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Feng
authors.forename: Ju-ying
authors.initials: JY
authors.lastname: Yan
authors.forename: Yi-yu
authors.initials: YY
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Shi
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Mao
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.forename: Bei-bei
authors.initials: BB
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D002681:China / Q000453:epidemiology; D005814:Genes, Viral; D005838:Genotype; D008457:Measles / Q000453:epidemiology; D008459:Measles virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny