In vitro antibacterial activity of bovine dialyzable leukocyte extract.
The rapidly developing resistance of many infectious pathogenic organisms to modern drugs has spurred scientists to search for new sources of antibacterial compounds. One potential candidate, bDLE (dialysis at 10 to 12 kDa cut-off) and its fractions ("S" and "L" by 3.5 kDa cut-off and I, II, III, and IV by molecular exclusion chromatography), was evaluated for antibacterial activity against pathogenic bacterial strains (Staphylococcus aureus, Streptococcus pyogenes, Lysteria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) using standard antimicrobial assays. A minimum inhibitory concentration (MIC) of bDLE and its fractions was determined by agar and broth dilutions methods. Only bDLE and its "S" fraction had an effect upon all bacteria evaluated (MIC ranging from 0.29 to 0.62 U/ml), and the bactericidal and bacteriostatic effects (evaluated by MTT assay) were bacterial species-dependent. These results showed a remarkable in vitro antibacterial property of bDLE against several pathogenic bacteria.
Armides Franco-Molina
Moisés
M
Departamento de Microbiología e Inmunología, Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Nuevo León, México.
Mendoza-Gamboa
Edgar
E
Castillo-Tello
Paloma
P
Tamez-Guerra
Reyes S
RS
Villarreal-Treviño
Licet
L
Tijerina-Menchaca
Rolando
R
Castillo-León
Leonardo
L
Zapata-Benavides
Pablo
P
Rodríguez-Padilla
Cristina
C
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D015169:Colony Count, Microbial; D015314:Dialysis Solutions / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006090:Gram-Negative Bacteria / Q000145:classification / Q000187:drug effects / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000145:classification / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D014165:Transfer Factor / Q000032:analysis / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D015314:Dialysis Solutions; D014165:Transfer Factor