title: Specific primers for rapid detection of Microsporum audouinii by PCR in clinical samples.
abstract: This report describes application of PCR fingerprinting to identify common species of dermatophytes using the microsatellite primers M13, (GACA)4, and (GTG)5. The initial PCR analysis rendered a specific DNA fragment for Microsporum audouinii, which was cloned and sequenced. Based on the sequencing data of this fragment, forward (MA_1F) and reverse (MA_1R) primers were designed and verified by PCR to establish their reliability in the diagnosis of M. audouinii. These primers produced a singular PCR band of 431 bp specific only to strains and isolates of M. audouinii, based on a global test of 182 strains/isolates belonging to 11 species of dermatophytes. These findings indicate these primers are reliable for diagnostic purposes, and we recommend their use in laboratory analysis.
authors.affiliation: Lab. Micologia, Instituto de Higiene e Medicina Tropical/CREM, Universidade Nova de Lisboa, Portugal.
authors.forename: H D
authors.initials: HD
authors.lastname: Roque
authors.forename: R
authors.initials: R
authors.lastname: Vieira
authors.forename: S
authors.initials: S
authors.lastname: Rato
authors.forename: M
authors.initials: M
authors.lastname: Luz-Martins
mesh_terms: D000293:Adolescent; D000328:Adult; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D003881:Dermatomycoses / Q000175:diagnosis / Q000382:microbiology*; D005260:Female; D006197:Hair / Q000382:microbiology; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008864:Microsporum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008875:Middle Aged; D009262:Nails / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology; D014006:Tinea Capitis / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal