Subcutaneous infusion and capillary "finger stick" sampling of stable isotope tracer in metabolic studies.
Metabolic studies utilizing stable isotope tracer in humans have typically used intravenous tracer infusions and venous blood sampling. These studies explore subcutaneous infusion of isotope and "finger stick" capillary blood sampling to measure glucose turnover. Five subjects received simultaneous 8-h infusions of glucose labeled with isotope: [1-(13)C]glucose subcutaneously and [6,6-(2)H(2)]glucose intravenously. At regular intervals, venous and finger stick blood specimens were obtained. Finger stick blood was applied to filter paper. Substrate and isotopic steady state was reached after 7.0 h with both routes of infusion. The isotopic enrichments of finger stick and venous specimens did not differ significantly for the subcutaneously infused [1-(13)C]glucose (p = 0.33 and p = 0.23, respectively) but the finger stick [6,6-(2)H(2)]glucose enrichment was slightly higher (p < 0.03) than that of the venous sample. Using [6,6-(2)H(2)]glucose infusion and venous plasma sampling as the reference method, the [1-(13)C]glucose gave estimates of glucose R(a) that were 13% (plasma) and 17% (finger stick) lower (p < 0.001 and p < 0.02, respectively). This difference could be attributed to recycling of (13)C label. In conclusion, subcutaneous infusion and finger stick specimen collection onto filter paper represent a potential method of conducting in vivo studies of substrate metabolism outside of a hospital-based research unit.
Hertel
Paula M
PM
Department of Pediatrics, Children's Nutrition Research Center, U.S. Department of Agriculture/Agricultural Research Service, Baylor College of Medicine, Houston, Texas 77030, USA.
Chacko
Shaji K
SK
Pal
Sunita
S
Sunehag
Agneta L
AL
Haymond
Morey W
MW
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D001800:Blood Specimen Collection / Q000379:methods*; D002196:Capillaries; D002247:Carbon Isotopes / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006801:Humans; D015918:Infusion Pumps, Implantable; D008297:Male; D011849:Radioactive Tracers; D015203:Reproducibility of Results
D001786:Blood Glucose; D002247:Carbon Isotopes; D011849:Radioactive Tracers