A novel approach to oligonucleotide synthesis using an imidazolium ion tag as a soluble support.
The synthesis of oligonucleotides in solution using a soluble, ionic liquid based support is described. Short oligomers of varying base composition were synthesized using this method in high yields and high purity, requiring no chromatography for purification prior to cleavage from the support. The solution-phase-synthesized oligomers were compared to the same sequences prepared using standard gene machine techniques by LCMS. This methodology may provide a cheaper route for the large-scale synthesis of oligonucleotides.
Donga
Robert A
RA
Department of Chemistry, McGill University, Montreal, Quebec, Canada H3A 2K6.
Khaliq-Uz-Zaman
Syed M
SM
Chan
Tak-Hang
TH
Damha
Masad J
MJ
D001482:Base Composition; D048288:Imidazolines / Q000737:chemistry*; D008722:Methods; D009841:Oligonucleotides / Q000138:chemical synthesis*; D012995:Solubility; D012996:Solutions
D048288:Imidazolines; D009841:Oligonucleotides; D012996:Solutions