title: Adjuvant and immunogenic activities of the 73kDa N-terminal alpha-domain of BrkA autotransporter and Cpn60/60kDa chaperonin of Bordetella pertussis.
abstract: A soluble fraction obtained from Bordetella pertussis was evaluated as adjuvant for the pertussis component of the Diphtheria-Pertussis-Tetanus (DPT) vaccine. High levels of antibodies were induced, and a 78% protection rate of mice challenged with live B. pertussis was observed. Two proteins were identified as the 73 kDa N-terminal alpha-domain of BrkA autotransporter protein and the Cpn60/60 kDa chaperonin. Both stimulated antibodies against pertussis and induced a 42% protection rate against the challenge. IgG1 and IgG2a were stimulated suggesting that the immune response could be modulated to produce Th1 or Th2.
authors.affiliation: Centro de Biotecnologia, Instituto Butantan, Av. Dr. Vital Brazil 1500, 05503-900 São Paulo, SP, Brazil. vcainelligebara@butantan.gov.br
authors.forename: Vera C B
authors.initials: VC
authors.lastname: Cainelli Gebara
authors.forename: Luciana
authors.initials: L
authors.lastname: Risoléo
authors.forename: Alexandre P Y
authors.initials: AP
authors.lastname: Lopes
authors.forename: Vera R F
authors.initials: VR
authors.lastname: Ferreira
authors.forename: Wagner
authors.initials: W
authors.lastname: Quintilio
authors.forename: François
authors.initials: F
authors.lastname: Lépine
authors.forename: Wilmar D
authors.initials: WD
authors.lastname: Silva
authors.forename: Isaías
authors.initials: I
authors.lastname: Raw
mesh_terms: D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology*; D018834:Chaperonin 60 / Q000276:immunology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010567:Pertussis Vaccine / Q000276:immunology*; D017434:Protein Structure, Tertiary; D014917:Whooping Cough / Q000276:immunology* / Q000382:microbiology
chemical_list: D000276:Adjuvants, Immunologic; D001425:Bacterial Outer Membrane Proteins; C090007:BrkA protein, Bordetella pertussis; D018834:Chaperonin 60; D010567:Pertussis Vaccine