title: Cumulative antioxidant defense against oxidative challenge in galactose-induced cataractogenesis in Wistar rats.
abstract: Natural dietary ingredients are known for their antioxidant activity. Of such, curcumin, the active principle of turmeric, at 0.01% in the diet proved as pro-oxidative in galactose-induced cataract in vivo. The purpose of this study was to investigate the effect of vitamin E (VE), a well-known antioxidant, in combination with curcumin on the onset and maturation of galactose induced cataract. Periodic slit-lamp microscope examination indicated that in combination with vitamin-E, 0.01% curcumin (G-IV) delayed the onset and maturation of galactose-induced cataract. Biochemical analyses revealed that combined treatment of 0.01% curcumin and vitamin-E diet exhibited an efficient antioxidant effect, as it inhibited lipid peroxidation and contributed to a distinct rise in reduced glutathione content. The results indicate that natural dietary ingredients are effective in combination rather than the individual administration as they are complementing each other in reducing the risk of galactose induced cataract.
authors.affiliation: Physiology Division, Department of Zoology, Osmania University, Hyderabad, India. rajutn57@yahoo.com
authors.forename: T N
authors.initials: TN
authors.lastname: Raju
authors.forename: C Sanat
authors.initials: CS
authors.lastname: Kumar
authors.forename: V Rajani
authors.initials: VR
authors.lastname: Kanth
authors.forename: B Venkata
authors.initials: BV
authors.lastname: Ramana
authors.forename: P Uma Maheswara
authors.initials: PU
authors.lastname: Reddy
authors.forename: P
authors.initials: P
authors.lastname: Suryanarayana
authors.forename: G Bhanuprakash
authors.initials: GB
authors.lastname: Reddy
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D003474:Curcumin / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005690:Galactose; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D008054:Lipid Peroxides; D014810:Vitamin E; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D003474:Curcumin; D005690:Galactose