Magnetic resonance visualization of tumor angiogenesis by targeting neural cell adhesion molecules with the highly sensitive gadolinium-loaded apoferritin probe.
Tumor vessel imaging could be useful in identifying angiogenic blood vessels as well as being a potential predictive marker of antiangiogenic treatment response. We recently reported the expression of the neural cell adhesion molecule (NCAM) in the immature and tumor endothelial cell (TEC) lining vessels of human carcinomas. Exploiting an in vivo model of human tumor angiogenesis obtained by implantation of TEC in Matrigel in severe combined immunodeficiency mice, we aimed to image angiogenesis by detecting the expression of NCAM with magnetic resonance imaging. The imaging procedure consisted of (a) targeting NCAMs with a biotinylated derivative of C3d peptide that is known to have high affinity for these epitopes and (b) delivery of a streptavidin/gadolinium (Gd)-loaded apoferritin 1:1 adduct at the biotinylated target sites. The remarkable relaxation enhancement ability of the Gd-loaded apoferritin system allowed the visualization of TEC both in vitro and in vivo when organized in microvessels connected to the mouse vasculature. Gd-loaded apoferritin displayed good in vivo stability and tolerability. The procedure reported herein may be easily extended to the magnetic resonance visualization of other epitopes suitably targeted by proper biotinylated vectors.
Geninatti Crich
Simonetta
S
Department of Chemistry IFM and Center for Molecular Imaging, University of Torino.
Bussolati
Benedetta
B
Tei
Lorenzo
L
Grange
Cristina
C
Esposito
Giovanna
G
Lanzardo
Stefania
S
Camussi
Giovanni
G
Aime
Silvio
S
D000818:Animals; D001052:Apoferritins / Q000737:chemistry / Q000378:metabolism; D001710:Biotin / Q000737:chemistry; D002292:Carcinoma, Renal Cell / Q000098:blood supply*; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D005682:Gadolinium / Q000737:chemistry; D007680:Kidney Neoplasms / Q000098:blood supply*; D018810:Magnetic Resonance Angiography / Q000379:methods*; D051379:Mice; D016513:Mice, SCID; D009389:Neovascularization, Pathologic / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D019006:Neural Cell Adhesion Molecules / Q000096:biosynthesis / Q000378:metabolism*; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism; D019809:Streptavidin / Q000737:chemistry
D019006:Neural Cell Adhesion Molecules; D009942:Organometallic Compounds; D001710:Biotin; D019809:Streptavidin; D001052:Apoferritins; D005682:Gadolinium