Aberrant FHIT protein expression in classical Hodgkin's lymphoma: a potential marker.
OBJECTIVE
The fragile histidine triad (FHIT) gene is frequently inactivated in human cancers; however, the FHIT gene remains unexplored in Hodgkin's lymphoma. The aim of this study was to investigate the role of FHIT expression in classical Hodgkin's lymphoma.


METHODS
Classical Hodgkin's lymphomas were analysed for FHIT gene expression by two-step non-biotin immunohistochemical method and Western blotting.


RESULTS
Thirty of the 33 (91%) cases of Hodgkin's lymphoma tested were positive for FHIT protein by immuohistochemistry. The expression of FHIT was mainly located in cytoplasm of Reed-Sternberg (HRS) cells. The protein expression was also documented by Western blotting. The non-Hodgkin's lymphomas were negative for FHIT protein.


CONCLUSIONS
The results indicate that abnormal FHIT expression is noted frequently in classical Hodgkin's lymphoma and the expression can give insight into the pathogenesis of the disease. The protein may serve as a marker to localise HRS cells in classical Hodgkin's lymphoma.
Zhao
Po
P
Department of Pathology, General Hospital of Chinese People's Liberation Army, Beijing, People's Republic of China. zhaopo@plagh.com.cn
Lam
Alfred King Yin
AK
Lu
Ya-Li
YL
Zhong
Mei
M
Chen
Ling-Hong
LH
Pu
Xiao-Lu
XL
D017766:Acid Anhydride Hydrolases / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D005260:Female; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D006689:Hodgkin Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D016539:Reed-Sternberg Cells / Q000378:metabolism / Q000473:pathology; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase