Dissecting drug and vehicle metabolic effects in rats by a metabonomic approach.
A combined application of high resolution (1)H NMR spectroscopy and multivariate statistical techniques focused on establishing a consistent statistical approach to metabonomic studies was tested. The data reduction, which is preliminary to the application of multivariate analysis to NMR spectra, was carried out by means of two complementary methods: pure Pattern Recognition (PR) and Assigned Signal Analysis (ASA). The simultaneous use of both approaches allowed us to obtain additional information in the analysis of metabonomic data, compared to the use of PR alone. This additional information consists in the possibility of a biochemical interpretation of the effects induced by treatment with xenobiotics, such as drugs or drug vehicles, on the metabolic networks of the systems under investigation. This approach allowed us to ascertain that a single-dose treatment with ST1959 vehicled by Sesame oil affects the production of hepatic glucose associated to an increment of the amino acid ketogenic process.
Colafranceschi
Mauro
M
Department of Chemistry, University of Rome La Sapienza, 00185 Rome, Italy.
Capuani
Giorgio
G
Miccheli
Alfredo
A
Campo
Silvia
S
Valerio
Mariacristina
M
Tomassini
Alberta
A
Giuliani
Alessandro
A
Arseni
Brunilde
B
Rossi
Stefania
S
De Santis
Rita
R
Carminati
Paolo
P
Ruggiero
Vito
V
Conti
Filippo
F
D000465:Algorithms; D000818:Animals; D005947:Glucose / Q000096:biosynthesis; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D015999:Multivariate Analysis; D010363:Pattern Recognition, Automated; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D014677:Pharmaceutical Vehicles / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D012715:Sesame Oil / Q000008:administration & dosage / Q000378:metabolism; D014230:Triazoles / Q000008:administration & dosage / Q000378:metabolism
D004364:Pharmaceutical Preparations; D014677:Pharmaceutical Vehicles; D014230:Triazoles; C030266:3-(2-ethylphenyl)-5-(3-methoxyphenyl)-1H-1,2,4-triazole; D012715:Sesame Oil; D005947:Glucose