Sensitive determination of carbohydrates labelled with p-nitroaniline by capillary electrophoresis with photometric detection using a 406 nm light-emitting diode.
p-Nitroaniline was explored as a derivatising reagent for UV absorbance detection of carbohydrates after separation by CE. This derivatising agent has three advantages: first, it has excellent water solubility; second, it has high molar absorptivity; and third, it is possible to obtain sensitive detection using a UV or blue light-emitting diode (LED) as the light source. The labelling reaction took less than 30 min to complete with high reaction yield. The separation process was modelled and optimised using an artificial neural network. Nine carbohydrates were separated by a CE system within 16 min using a 0.17 M boric acid buffer at pH 9.7. On-column LED detection at 406 nm allowed the detection of carbohydrates with good detection limits (<1.1 microM or 8.8 fmol) and reproducible quantification in the concentration range of 2.6-200 microM. This method was applied successfully to the determination of component carbohydrates in some food samples.
Momenbeik
Fariborz
F
Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Tasmania, Australia.
Johns
Cameron
C
Breadmore
Michael C
MC
Hilder
Emily F
EF
Macka
Miroslav
M
Haddad
Paul R
PR
D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D001888:Boric Acids; D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D002253:Carbonated Beverages / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D005947:Glucose / Q000032:analysis; D006801:Humans; D007223:Infant; D041943:Infant Formula / Q000737:chemistry; D007785:Lactose / Q000032:analysis; D008320:Maltose / Q000032:analysis; D008892:Milk / Q000737:chemistry; D012275:Oryza / Q000737:chemistry; D010783:Photometry; D013056:Spectrophotometry, Ultraviolet
D000814:Aniline Compounds; D001888:Boric Acids; D002241:Carbohydrates; C008625:nitroaniline; D008320:Maltose; D005947:Glucose; D007785:Lactose; C032688:boric acid