title: Gene-gene interaction between IL-13 and IL-13Ralpha1 is associated with total IgE in Korean children with atopic asthma.
abstract: Interleukin (IL)-13, which is essential for IgE synthesis, mediates its effects by binding with a receptor composed of IL-4Ralpha and IL-13Ralpha1. We investigated the effects of IL-13 and IL-13Ralpha1 polymorphisms in Korean children with asthma, and whether these have been associated with IgE production. We enrolled 358 atopic asthmatic, 111 non-atopic asthmatic, and 146 non-atopic healthy children. IL-13 and IL-13Ralpha1 genotypes were identified using the PCR-RFLP method. There was an association between the asthma susceptibility and homozygosity for risk allele of IL-13 G+2044A. In children with atopic asthma, risk alleles in IL-13 (A-1512C and C-1112T) and IL-13Ralpha1 (A+1398G) showed increased total IgE (P=0.012, 0.015 and 0.017, respectively). Three-loci haplotype analysis for IL-13 showed that the haplotype composed of -1512C, -1112T and +2044A was associated with higher total IgE than other tested haplotypes in children with atopic asthma (P=0.003). The gene-gene interaction between risk alleles of each IL-13 promoter polymorphism and IL-13Ralpha1 polymorphism was associated with higher total IgE in children with atopic asthma (P=0.002, 0.010). These findings indicate that the IL-13 G+2044A is associated with asthma development and the IL-13 and IL-13Ralpha1 polymorphisms may interact to enhance IgE production.
authors.affiliation: Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, 388-1 Pungnap-dong Songpa-gu, Seoul, 138-736, Korea.
authors.forename: Hyo-Bin
authors.initials: HB
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine and Research Center for Allergic Immune Diseases, Chonbuk National University Medical School, Jeonju, Korea.
authors.forename: Yong-Chul
authors.initials: YC
authors.lastname: Lee
authors.affiliation: Department of Pediatrics, Hangang Sacred Heart Hospital, University of Hallym College of Medicine, Seoul, Korea.
authors.forename: So-Yeon
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Genomic Epidemiology and Bioinformatics Division, National Genome Research Institute, Korean National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Korea.
authors.forename: Jongsun
authors.initials: J
authors.lastname: Jung
authors.affiliation: Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, 388-1 Pungnap-dong Songpa-gu, Seoul, 138-736, Korea.
authors.forename: Hyun-Seung
authors.initials: HS
authors.lastname: Jin
authors.affiliation: Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, 388-1 Pungnap-dong Songpa-gu, Seoul, 138-736, Korea.
authors.forename: Ja-Hyeung
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, 388-1 Pungnap-dong Songpa-gu, Seoul, 138-736, Korea.
authors.forename: Bong-Seong
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Asan Institute for Life Science, Asan Medical Center, Seoul, Korea.
authors.forename: Mi-Jin
authors.initials: MJ
authors.lastname: Kang
authors.affiliation: Asan Institute for Life Science, Asan Medical Center, Seoul, Korea.
authors.forename: Seong-Ok
authors.initials: SO
authors.lastname: Jang
authors.affiliation: Genomic Epidemiology and Bioinformatics Division, National Genome Research Institute, Korean National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Korea.
authors.forename: Jihong
authors.initials: J
authors.lastname: Kim
authors.affiliation: Genomic Epidemiology and Bioinformatics Division, National Genome Research Institute, Korean National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Korea.
authors.forename: Kuchan
authors.initials: K
authors.lastname: Kimm
authors.affiliation: Statistical Research Institute, Dongguk University, Seoul, Korea.
authors.forename: Eun-Soon
authors.initials: ES
authors.lastname: Shin
authors.affiliation: Department of Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea.
authors.forename: Seong-Gene
authors.initials: SG
authors.lastname: Lee
authors.affiliation: Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, 388-1 Pungnap-dong Songpa-gu, Seoul, 138-736, Korea. sjhong@amc.seoul.kr.
authors.forename: Soo-Jong
authors.initials: SJ
authors.lastname: Hong
mesh_terms: D044466:Asian People; D001249:Asthma / Q000235:genetics* / Q000276:immunology; D002648:Child; D005260:Female; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D018793:Interleukin-13 / Q000235:genetics* / Q000378:metabolism; D053661:Interleukin-13 Receptor alpha1 Subunit / Q000235:genetics* / Q000378:metabolism; D008297:Male; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012130:Respiratory Hypersensitivity / Q000235:genetics / Q000276:immunology
chemical_list: D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; D007073:Immunoglobulin E