Use of the CellCard System for analyzing multiple cell types in parallel.
The CellCard system enables the analysis of multiple cell types within a single microtiter well. In doing so, the CellCard system not only determines the effect of an experimental condition on a cell type of interest, but also the relative selectivity of that response across nine other cell types. In addition, this approach of cellular multiplexing is a means of miniaturization without the necessity of microfluidic devices. The standard 96-well plate generates ten 96-well plates of data (or, the equivalent of a 960-well plate). Taken together, the CellCard technology enables multiple cell types to be assayed within a single microtiter well allowing for the simultaneous determination of cellular activity and compound selectivity. This chapter will describe a method by which multiple cell types can be simultaneously assayed for biological parameters of interest.
Beske
Oren
O
Vitra Bioscience, Inc., Mountain View, CA, USA.
Bassoni
Daniel
D
Goldbard
Simon
S
D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D007091:Image Processing, Computer-Assisted; D013194:Staining and Labeling; D046888:Tissue Array Analysis / Q000295:instrumentation / Q000379:methods*