Use of DNA arrays for the analysis of outbreak-related strains of Listeria monocytogenes.
We evaluated the discriminative power and usefulness of the DNA array technology as compared to DNA macrorestriction pattern analysis for monitoring epidemiologically related clusters of Listeria monocytogenes strains that differ slightly in DNA macrorestriction patterns. We show that this approach allows clarifying the genetic basis of the pattern variations. In the reported outbreak, the differences were due to phage excision, showing the power of this technique in epidemiological studies.
Doumith
Michel
M
Laboratoire des Listeria, Centre National de Référence des Listeria, World Health Organisation Collaborating Center for Foodborne Listeriosis, Institut Pasteur, 25-28 rue du Dr Roux, F-75724 Paris Cedex 15, France.
Jacquet
Christine
C
Goulet
Véronique
V
Oggioni
Chiara
C
Van Loock
Frank
F
Buchrieser
Carmen
C
Martin
Paul
P
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics; D008088:Listeriosis / Q000453:epidemiology* / Q000382:microbiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015183:Restriction Mapping
D004269:DNA, Bacterial