Antioxidant redox sensors based on DNA modified carbon screen-printed electrodes.
Antioxidant redox sensors based on DNA modified carbon screen-printed electrodes were developed. The carbon ink was doped with TiO2 nanoparticles, onto which double-strand DNA was adsorbed. A redox mediator, namely, tris-2,2'-bipyridine ruthenium(II) [Ru(bpy)3(2+)] was electrooxidized on the electrode surface to subsequently oxidize both the adsorbed ds-DNA and the antioxidants in solution. The resulting oxidation damage of the adsorbed ds-DNA was then detected by square wave voltammetry in a second solution containing only Ru(bpy)3Cl2 at a low concentration (microM). A kinetic model was developed to study the protecting role of antioxidants in aqueous solutions. The electrochemical sensor has been applied to evaluate the redox antioxidant capacity of different molecules.
Liu
Jifeng
J
Laboratoire d'Electrochimie Physique et Analytique, Station 6, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
Su
Bin
B
Lagger
Grégoire
G
Tacchini
Philippe
P
Girault
Hubert H
HH
D000975:Antioxidants / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004566:Electrodes; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D014025:Titanium / Q000737:chemistry
D000975:Antioxidants; C009495:titanium dioxide; D002244:Carbon; D004247:DNA; D014025:Titanium