Distribution of pancreatic endocrine cells including IAPP-expressing cells in non-diabetic and type 2 diabetic cases.
There is a lack of agreement on the distribution of islet amyloid polypeptide (IAPP) in the pancreases of healthy and diabetic subjects. Therefore, a detailed morphometrical and immunohistochemical study was performed to obtain information on the distribution of cells expressing insulin, glucagon, somatostatin, pancreatic polypeptide (PP), and IAPP in the pancreases of non-diabetic (n=4) and diabetic individuals (n=6). In the non-diabetic cases, beta-cells contributed to approximately 64%, alpha-cells to 26%, delta-cells to 8%, PP cells to 0.3%, and IAPP cells to 34% of the islet cell population. The ratio of IAPP/insulin was approximately 1:2. In diabetic cases, beta-cells were decreased by 24%, and IAPP was decreased by 57%. The alpha- and delta-cells were increased by 40% and 58%, respectively. IAPP/insulin ratio was decreased by 41%. Thus, only 50% of the beta-cells in non-diabetics and only 30% in diabetics coexpressed IAPP. In diabetics, more delta-cells coexpressed IAPP than in non-diabetics. The results seem to argue against the notion that the secretion of IAPP is increased in diabetics. It is possible that an increase in somatostatin and glucagon plays a greater role in diabetes than IAPP.
Iki
Katsumichi
K
UNMC Eppley Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198-6805, USA.
Pour
Parviz M
PM
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000682:Amyloid / Q000096:biosynthesis*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005934:Glucagon / Q000096:biosynthesis; D050416:Glucagon-Secreting Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007328:Insulin / Q000096:biosynthesis; D050417:Insulin-Secreting Cells / Q000378:metabolism / Q000473:pathology; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010191:Pancreatic Polypeptide / Q000096:biosynthesis; D050418:Pancreatic Polypeptide-Secreting Cells / Q000378:metabolism / Q000473:pathology; D012016:Reference Values; D013004:Somatostatin / Q000096:biosynthesis; D019864:Somatostatin-Secreting Cells / Q000378:metabolism / Q000473:pathology
D000682:Amyloid; D007328:Insulin; D058228:Islet Amyloid Polypeptide; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D005934:Glucagon