title: Use of DNA arrays for the analysis of outbreak-related strains of Listeria monocytogenes.
abstract: We evaluated the discriminative power and usefulness of the DNA array technology as compared to DNA macrorestriction pattern analysis for monitoring epidemiologically related clusters of Listeria monocytogenes strains that differ slightly in DNA macrorestriction patterns. We show that this approach allows clarifying the genetic basis of the pattern variations. In the reported outbreak, the differences were due to phage excision, showing the power of this technique in epidemiological studies.
authors.affiliation: Laboratoire des Listeria, Centre National de Référence des Listeria, World Health Organisation Collaborating Center for Foodborne Listeriosis, Institut Pasteur, 25-28 rue du Dr Roux, F-75724 Paris Cedex 15, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Doumith
authors.forename: Christine
authors.initials: C
authors.lastname: Jacquet
authors.forename: Véronique
authors.initials: V
authors.lastname: Goulet
authors.forename: Chiara
authors.initials: C
authors.lastname: Oggioni
authors.forename: Frank
authors.initials: F
authors.lastname: Van Loock
authors.forename: Carmen
authors.initials: C
authors.lastname: Buchrieser
authors.forename: Paul
authors.initials: P
authors.lastname: Martin
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics; D008088:Listeriosis / Q000453:epidemiology* / Q000382:microbiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial