Characterization and application of aptamers for Taq DNA polymerase selected using an evolution-mimicking algorithm.
Using an evolution-mimicking algorithm (EMA), we have recently identified DNA aptamers that inhibit Taq DNA polymerase. In the present study, we have attempted to improve further the inhibitory activities of aptamers, as well as to characterize those aptamers with the most potent inhibitory activities. To characterize the most potent aptamer and demonstrate its applicability, the abilities to inhibit Tth DNA polymerase and to modulate specific amplification in PCR were investigated. This aptamer inhibited both Tth DNA polymerase and Taq DNA polymerase and improved the specificity of detection of a low-copy-number target gene in PCR using these DNA polymerases.
Noma
Takahisa
T
Department of Life Science and Biotechnology, Tokyo University of Agriculture and Technology, Naka-cho, Koganei, Tokyo, Japan.
Sode
Koji
K
Ikebukuro
Kazunori
K
D000465:Algorithms; D052157:Aptamers, Nucleotide; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D004279:DNA, Viral / Q000235:genetics; D019143:Evolution, Molecular; D015497:HIV-1 / Q000235:genetics; D008024:Ligands; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase / Q000737:chemistry* / Q000235:genetics*
D052157:Aptamers, Nucleotide; D004279:DNA, Viral; D008024:Ligands; D009841:Oligonucleotides; D019914:Taq Polymerase