Thioredoxin target proteins in chloroplast thylakoid membranes.
In recent years, impressive progress has been made in the identification of thioredoxin-linked proteins. However, due to technical difficulties inherent in working with hydrophobic proteins, identifications so far have been restricted to proteins in the soluble fraction. Thus, our knowledge of redox regulated membrane proteins is quite limited. To gain information in this area, the authors have applied an adaptation of the approach based on the fluorescent thiol probe monobromobimane (mBBr) to identify redox-linked proteins of chloroplast thylakoids. By application of this procedure, 14 potential membrane-bound thioredoxin target proteins were identified, including seven new candidates functional in processes associated with photosynthetic electron flow, ATP synthesis, and Photosystem II/Photosystem I state transitions.
Balmer
Yves
Y
Department of Plant and Microbial Biology, University of California, Berkeley, 94720, USA.
Vensel
William H
WH
Hurkman
William J
WJ
Buchanan
Bob B
BB
D001643:Bridged Bicyclo Compounds / Q000737:chemistry; D025222:Chloroplast Proton-Translocating ATPases / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005033:Ethylmaleimide / Q000737:chemistry; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D007460:Iodoacetamide / Q000737:chemistry; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D045331:Photosystem I Protein Complex / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D018724:Spinacia oleracea / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism*; D020524:Thylakoids / Q000378:metabolism*
D001643:Bridged Bicyclo Compounds; D045222:Electron Transport Chain Complex Proteins; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; C054765:rca protein, plant; D013879:Thioredoxins; D025222:Chloroplast Proton-Translocating ATPases; D012273:Ribulose-Bisphosphate Carboxylase; D005634:Fructose-Bisphosphate Aldolase; D005033:Ethylmaleimide; C028381:monobromobimane; D007460:Iodoacetamide