[Preliminary approach towards construction of peptide libraries as potential tools for diagnosis of malignant thyroid tumors].
BACKGROUND
Cancer of thyroid gland is the most common malignancy of the endocrine system. The treatment improvement could be achieved by early diagnosis. The aim of the study was to identify cancer specific antigenes with use of peptide libraries.


METHODS
The material from 6 patients with thyroid cancer (4 with papillary cancer, 1 with follicular cancer and 1 with oxyphilic tumor) were analyzed. It was performed with use of lipophylic peptide libraries by direct comparison of staining of specimens prepared from normal and malignant tissue.


RESULTS
Preliminary results confirm practical value of peptide libraries in early diagnostics of thyroid cancer.


CONCLUSIONS
It is important to optimize construction of peptide libraries by using different staining agents hydrolyzed by proteases.
Balcerzak
Waldemar
W
1st Department of General, Gastroenterological and Endocrinological Surgery, Medical University, Wroclaw. wbalcerzak@wp.pl
Bednarz
Wiktor
W
Domosławski
Paweł
P
Olewiński
Robert
R
Kolesińska
Justyna
J
Kaminski
Zbigniew
Z
Dziarkowska
Katarzyna
K
Wieczorek
Piotr
P
D018263:Adenocarcinoma, Follicular / Q000737:chemistry / Q000473:pathology*; D018249:Adenoma, Oxyphilic / Q000737:chemistry / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D018265:Carcinoma, Papillary, Follicular / Q000737:chemistry / Q000473:pathology*; D005240:Feasibility Studies; D005260:Female; D037502:Galectin 3 / Q000032:analysis; D006801:Humans; D007453:Iodide Peroxidase / Q000032:analysis; D008297:Male; D009361:Neoplasm Invasiveness; D019151:Peptide Library; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D013964:Thyroid Neoplasms / Q000737:chemistry / Q000473:pathology*
D014408:Biomarkers, Tumor; D037502:Galectin 3; D019151:Peptide Library; D007453:Iodide Peroxidase