Single nanoparticle photothermal tracking (SNaPT) of 5-nm gold beads in live cells.
Tracking individual nano-objects in live cells during arbitrary long times is a ubiquitous need in modern biology. We present here a method for tracking individual 5-nm gold nanoparticles on live cells. It relies on the photothermal effect and the detection of the Laser Induced Scattering around a NanoAbsorber (LISNA). The key point for recording trajectories at video rate is the use of a triangulation procedure. The effectiveness of the method is tested against single fluorescent molecule tracking in live COS7 cells on subsecond timescales. We further demonstrate recordings for several minutes of AMPA receptors trajectories on the plasma membrane of live neurons. Single Nanoparticle Photothermal Tracking has the unique potential to record arbitrary long trajectory of membrane proteins using nonfluorescent nanometer-sized labels.
Lasne
David
D
Centre de Physique Moléculaire Optique et Hertzienne, CNRS (UMR 5798) and Université Bordeaux 1, Talence Cedex, France.
Blab
Gerhard A
GA
Berciaud
Stéphane
S
Heine
Martin
M
Groc
Laurent
L
Choquet
Daniel
D
Cognet
Laurent
L
Lounis
Brahim
B
D000818:Animals; D001692:Biological Transport; D019556:COS Cells; D002232:Carbocyanines; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005456:Fluorescent Dyes; D006046:Gold / Q000737:chemistry*; D007834:Lasers; D008027:Light; D053768:Metal Nanoparticles; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000378:metabolism; D017470:Receptors, Glutamate / Q000378:metabolism; D012542:Scattering, Radiation; D013696:Temperature
D002232:Carbocyanines; D005456:Fluorescent Dyes; D017470:Receptors, Glutamate; C085321:cyanine dye 5; D006046:Gold