Composition of the volatile fraction of Ocotea bofo Kunth (Lauraceae) calyces by GC-MS and NMR fingerprinting and its antimicrobial and antioxidant activity.
The chemical composition of the essential oil obtained by steam distillation of the floral calyces of Ocotea bofo Kunth (Lauraceae) was studied by means of GC, GC-MS, and 1H, 13C, and bidimensional NMR (COSY, HSQC, HMBC). Twenty-five constituents were identified, and estragole (48.7%), alpha-phellandrene (19.6%) and sabinene (10.4%) were found to be the major components. Antimicrobial activity against six aerobic bacteria and five yeasts and antioxidant activity performed by photochemiluminescence (PCL), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and beta-carotene bleaching assays are reported. The oil showed fair inhibiting properties against bacteria and a good inhibition against most yeasts. Its radical scavenging and chain-breaking antioxidant properties were comparable to or better than those provided by synthetic controls. Particular emphasis has been given to the use of NMR as a fast and reliable tool to discriminate O. bofo essential oil from other commercial anethole- and estragole-rich oils, namely, Illicium verum, Foeniculum vulgare, and Artemisia dracunculus.
Guerrini
Alessandra
A
Dipartimento delle Risorse Naturali e Culturali, Università degli Studi di Ferrara, Corso Ercole d'Este 32, 44100 Ferrara, Italy.
Sacchetti
Gianni
G
Muzzoli
Mariavittoria
M
Moreno Rueda
Gabriela
G
Medici
Alessandro
A
Besco
Elena
E
Bruni
Renato
R
D000081248:Allylbenzene Derivatives; D000840:Anisoles / Q000032:analysis / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D035264:Flowers / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D027444:Ocotea / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology; D010851:Picrates; D014835:Volatilization; D019207:beta Carotene / Q000737:chemistry
D000081248:Allylbenzene Derivatives; D000840:Anisoles; D000890:Anti-Infective Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D009822:Oils, Volatile; D010851:Picrates; D019207:beta Carotene; C007633:estragole; C004931:1,1-diphenyl-2-picrylhydrazyl