title: Hippocampus protein profiling reveals aberration of malate dehydrogenase in chlorpromazine/clozapine treated rats.
abstract: Chlorpromazine and clozapine are antipsychotic agents used to relieve symptoms of early-diagnosed schizophrenia. In this study, we used proteomics technology to screen the protein aberration in Sprague-Dawley rats treated with these two antipsychotic agents. Our goal was to identify the effects of antipsychotics on hippocampal proteins in rats. Protein expression profiles were compared in each experimental group using two-dimensional gel electrophoresis and identified using matrix-assisted laser desorption/ionisation time of flight mass spectrometry. Malate dehydrogenase, peroxiredoxin 3, vacuolar ATP synthase subunit beta and mitogen-activated protein kinase kinase 1 were found to have altered expression levels in the groups treated with antipsychotics compared with the matched controls. These findings should contribute to identifying new targets for disease preventing pharmacological agents and be beneficial for the development of more effective agents.
authors.affiliation: Bio-X Center, Shanghai Jiao Tong University, Haoran Building, 1954 Huashan Road, Shanghai 200030, PR China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: La
authors.forename: Chunling
authors.initials: C
authors.lastname: Wan
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Yang
authors.forename: Yongshuo
authors.initials: Y
authors.lastname: Chen
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Pan
authors.forename: Baohu
authors.initials: B
authors.lastname: Ji
authors.forename: Guoyin
authors.initials: G
authors.lastname: Feng
authors.forename: Lin
authors.initials: L
authors.lastname: He
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology*; D002746:Chlorpromazine / Q000494:pharmacology*; D003024:Clozapine / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology; D008291:Malate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D040081:Protein Array Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D014150:Antipsychotic Agents; D008291:Malate Dehydrogenase; D003024:Clozapine; D002746:Chlorpromazine