Bacterial artificial chromosome transgenic analysis of dynamic expression patterns of regulator of G-protein signaling 4 during development. II. Subcortical regions.
A large family of regulator of G protein signaling (RGS) proteins modulates signaling through G-protein-coupled receptors. Previous studies have implicated RGS4 as a vulnerability gene in schizophrenia. To begin to understand structure-function relationships, we have utilized bacterial artificial chromosome (BAC) methods to create transgenic mice that express green fluorescent protein (GFP) under the control of endogenous RGS4 enhancer elements, circumventing the lack of suitable antibodies for analysis of dynamic patterns of expression. This report follows from the accompanying mapping paper in cerebral cortex, with a focus on developmental and mature expression patterns in subcortical telencephalic, diencephalic and brainstem areas. Based on reporter distribution, the data suggest that alterations in RGS4 function will engender a complex phenotype of increased and decreased neuronal output, with developmental, regional, and cellular specificity.
Ebert
P J
PJ
Department of Pharmacology, Vanderbilt University School of Medicine, 465 21st Ave South, MRB III, Room 8114, Nashville, TN 37232, USA. Philip.Ebert@Vanderbilt.edu
Campbell
D B
DB
Levitt
P
P
D000375:Aging / Q000502:physiology; D000818:Animals; D001921:Brain / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008967:Molecular Biology / Q000379:methods*; D009434:Neural Pathways / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D020710:RGS Proteins / Q000235:genetics*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D019076:Transgenes / Q000235:genetics
D020710:RGS Proteins; D049452:Green Fluorescent Proteins; C098799:RGS4 protein