Preparation of platelet-rich plasma as a tissue adhesive for experimental transplantation in rabbits.
OBJECTIVE
Platelet-rich plasma (PRP) is an autologous substance with adhesive properties. We aimed at developing and testing the efficacy of a method for PRP preparation in rabbits.


METHODS
An in vitro study was carried out to obtain PRP from forty rabbits and to analyze the number of platelets and type of substance needed to trigger platelet activation. To induce platelet activation, 5%, 10%, 25% and 50% CaCl solutions were used. Then, an in vivo study was performed in twelve rabbits to test PRP adhesiveness in lamellar corneal graft. A control group made up of six rabbits underwent corneal transplantation without using PRP.


RESULTS
5% CaCl was the most effective concentration in activating PRP, with a mean time of 19 minutes. An attached corneal flap was seen 3 months after surgery. A detached corneal button was seen in all controls.


CONCLUSIONS
Our method was able to produce rabbit-derived PRP with suitable properties for soft tissue adhesion. These results could be useful for researchers of the growing fields of tissue repair and experimental transplantation.
Luengo Gimeno
Federico
F
Department of Ophthalmology, Facultad de Ciencias Biomédicas, Universidad Austral, Av. Perón 1500, Pilar, B1629AHJ, Argentina. fluengogimeno@fibertel.com.ar
Gatto
Silvia
S
Ferro
José
J
Croxatto
Juan Oscar
JO
Gallo
Juan Eduardo
JE