Effects of probiotic bacteria on gastrointestinal motility in guinea-pig isolated tissue.
OBJECTIVE
To evaluate the intestinal motility changes evoked by 8 bacterial strains belonging to Bifidobacterium, Lactobacillus and Streptococcus genera within the probiotic preparation VSL#3.


METHODS
Ileum and proximal colon segments isolated from guinea-pigs were used as a study model. Entire cells and cell fractions (cell debris, cell wall fraction, cytoplasmatic fraction, proteinaceous and non-proteinaceous cytoplasmatic components) of VSL#3 strains and, as controls, Escherichia coli, Salmonella aboni and Bacillus licheniformis were tested in this in vitro model.


RESULTS
Among the bacterial cell fractions tested, only the cytoplasmatic fraction modified intestinal motility. Lactobacillus strains stimulated the contraction of ileum segment, whereas all probiotic strains tested induced proximal colon relaxation response. The non-proteinaceous cytoplasmatic components were responsible for the colon relaxation.


CONCLUSIONS
The results obtained in this study suggest that the proximal colon relaxation activity showed by the probiotic bacteria could be one of the possible mechanisms of action by which probiotics exert their positive effects in regulating intestinal motility.
Massi
Matteo
M
Department of Pharmaceutical Sciences, University of Bologna, Italy.
Ioan
Pierfranco
P
Budriesi
Roberta
R
Chiarini
Alberto
A
Vitali
Beatrice
B
Lammers
Karen M
KM
Gionchetti
Paolo
P
Campieri
Massimo
M
Lembo
Anthony
A
Brigidi
Patrizia
P
D000818:Animals; D001644:Bifidobacterium / Q000502:physiology*; D003106:Colon / Q000382:microbiology / Q000502:physiology; D005260:Female; D005769:Gastrointestinal Motility / Q000187:drug effects* / Q000502:physiology; D006168:Guinea Pigs; D007082:Ileum / Q000382:microbiology / Q000502:physiology; D007778:Lactobacillus / Q000502:physiology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology; D019936:Probiotics / Q000494:pharmacology*; D013291:Streptococcus / Q000502:physiology*