Efficient reactivation of latent herpes simplex virus from mouse central nervous system tissues.
For decades, numerous ex vivo studies have documented that latent herpes simplex virus (HSV) reactivates efficiently from ganglia, but rarely from the central nervous systems (CNS), of mice when assayed by mincing tissues before explant culture, despite the presence of viral genomes in both sites. Here we show that 88% of mouse brain stems reactivated latent virus when they were dissociated into cell suspensions before ex vivo explant culture. The efficient reactivation of HSV from the mouse CNS was demonstrated with more than one viral strain, viral serotype, and mouse strain, further indicating that the CNS can be an authentic latency site for HSV with the potential to cause recurrent disease.
Chen
Shih-Heng
SH
Department of Microbiology and Immunology, Medical College, National Cheng Kung University, Tainan, Taiwan 701, Republic of China.
Yao
Hui-Wen
HW
Huang
Wen-Yen
WY
Hsu
Kuei-Sen
KS
Lei
Huan-Yao
HY
Shiau
Ai-Li
AL
Chen
Shun-Hua
SH
D000818:Animals; D018929:Cell Culture Techniques; D002490:Central Nervous System / Q000502:physiology / Q000821:virology*; D051379:Mice; D018139:Simplexvirus / Q000502:physiology*; D014775:Virus Activation / Q000502:physiology*; D017735:Virus Latency / Q000502:physiology*