Purification and properties of an unusual UDP-glucose dehydrogenase, NADPH-dependent, from Xanthomonas albilineans.
Xanthomonas albilineans produces a UDP-glucose dehydrogenase growing on sucrose. The enzyme oxidizes UDP-glucose to UDP-glucuronic acid by using molecular oxygen and NADPH. Kinetics of enzymatic oxydation of NADPH is linearly dependent on the amount of oxygen supplied. The enzyme has been purified at homogeneity. The value of pI of the purified enzyme is 8.98 and its molecular mass has been estimated as about 14 kDa. The enzyme shows a michaelian kinetics for UDP-glucose concentrations. The value of K(m) for UDP-glucose is 0.87 mM and 0.26 mM for NADPH, although the enzyme has three different sites to interact with NADPH. The enzyme is inhibited by UDP-glucose concentrations higher than 1.3 mM. N-Terminal sequence has been determined as IQPYNH.
Blanch
María
M
Laboratory of Plant Physiology, Faculty of Biology, Complutense University, Madrid, Spain.
Legaz
María-Estrella
ME
Vicente
C
C
D004791:Enzyme Inhibitors / Q000494:pharmacology; D007526:Isoelectric Point; D007700:Kinetics; D008970:Molecular Weight; D009249:NADP / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D014532:Uridine Diphosphate Glucose / Q000378:metabolism / Q000494:pharmacology; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D014974:Xanthomonas / Q000201:enzymology*
D004791:Enzyme Inhibitors; D009249:NADP; D014533:Uridine Diphosphate Glucose Dehydrogenase; D014532:Uridine Diphosphate Glucose