Three methods for the introduction of foreign DNA into Agrobacterium.
The genetic manipulation of Agrobacterium tumefaciens is used to facilitate studies of bacterial gene functions or as a first step in introducing genetic material into transformable plant cells through the use of T-DNA binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done with either electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into Agrobacterium using the triparental mating method. Here we present three detailed protocols for Agrobacterium strain construction using electroporation, the freeze/thaw method of transformation, and triparental mating.
Wise
Arlene A
AA
Plant Science Institute, Department of Biology, University of Pennsylvania, PA, USA.
Liu
Zhenying
Z
Binns
Andrew N
AN
D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D010944:Plants / Q000235:genetics / Q000382:microbiology; D010957:Plasmids; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; C036483:T-DNA