Determination of ligand-binding constants by isoelectric focusing. Generalization and extension of theory.
The theory of a proposed isoelectric focusing procedure for the determination of ligand binding constants has been generalized (via numerical calculations) to non-linear, electrophoretic velocity profiles and systems for which the change in velocity of the protein accompanying ligand binding depends upon pH. The theory has also been extended to include binding of ligand to heterogeneous sites. Determination of accurate values of the binding parameters depends upon extrapolation of apparent parameters to infinite dilution of protein.
Cann
J R
JR
Department of Biophysics and Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA.