title: Paradoxically enhanced heart tolerance to ischaemia in type 1 diabetes and role of increased osmolarity.
abstract: There is considerable controversy regarding the tolerance of diabetic hearts to ischaemia and the underlying mechanisms responsible for the increased heart tolerance to ischamia remain uncertain. In the present study, we observed, in vitro, type 1 diabetic heart responses to ischaemia and reperfusion at different degrees of hyperglycaemia. In addition, the possible role of increased osmolarity in cardioprotection due to hyperglycaemia was evaluated. Hearts from 3 week streptozocin-induced diabetic rats were isolated and perfused in a Langendorff apparatus and subjected to 30 min ischaemia and 30 min reperfusion. Cardiac function and the electrocardiogram were recorded. Myocardial content of osmolarity associated heat shock protein (hsp) 90, heme oxygenase (HO)-1 and anti-oxidant enzymes were determined in diabetic or hyperosmotic solution-perfused hearts using western blot. The hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin (17-AAG; 2 x 10(-7) mol/L) or the nitric oxide synthase (NOS) inhibitor Nomega-nitro-L-arginine methyl ester (1 x 10(-5) mol/L) was added to the perfusate to observe the effects of hsp90 inhibition and hsp90-associated endothelial NOS on ischaemic responses of diabetic hearts. Compared with normal control rats, diabetic hearts with severe hyperglycaemia (blood glucose > 20 mmol/L) showed markedly improved postischaemic heart function with fewer reperfusion arrhythmias. Mild hyperglycaemia (< 12 mmol/L) exhibited no significant cardioprotection. Elevated expression of hsp90 accompanied the enhanced resistance to ischaemia in diabetic hearts, which was abrogated by 17-AAG. In the presence of the NOS inhibitor, heart function was preserved, whereas reperfusion arrhythmias were increased in diabetes. Diabetic hearts also had markedly elevated HO-1 and catalase, with no significant change in superoxide dismutase. Hyperosmotic perfusion with glucose or mannitol also increased myocardial hsp90 and catalase. The present findings reveal that heart resistance to ischaemia is increased in short-term type 1 diabetes with severe hyperglycaemia. Elevated osmolarity caused by significant hyperglycaemia may contribute to the enhanced myocardial activity against oxidative injury during ischaemia and reperfusion.
authors.affiliation: Department of Pharmacology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. hchen100@citiz.net
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Wei-Li
authors.initials: WL
authors.lastname: Shen
authors.forename: Xu-Hui
authors.initials: XH
authors.lastname: Wang
authors.forename: Hong-Zhuan
authors.initials: HZ
authors.lastname: Chen
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Gu
authors.forename: Jie
authors.initials: J
authors.lastname: Fu
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Ni
authors.forename: Ping-Jin
authors.initials: PJ
authors.lastname: Gao
authors.forename: Ding-Liang
authors.initials: DL
authors.lastname: Zhu
authors.forename: Hideaki
authors.initials: H
authors.lastname: Higashino
mesh_terms: D000818:Animals; D001786:Blood Glucose; D003921:Diabetes Mellitus, Experimental / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000139:chemically induced / Q000473:pathology*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D006943:Hyperglycemia / Q000473:pathology; D066298:In Vitro Techniques; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods; D008297:Male; D017202:Myocardial Ischemia / Q000097:blood / Q000473:pathology*; D009994:Osmolar Concentration; D018384:Oxidative Stress; D051381:Rats; D015424:Reperfusion / Q000379:methods
chemical_list: D001786:Blood Glucose; D018841:HSP90 Heat-Shock Proteins