[Experimental studies on ectopic osteogenesis by seeding human mesenchymal stem cell on human natural bone derived scaffold materials].
OBJECTIVE
To investigate the ectopic osteogenesis potential of human natural bone derived material combined with human bone marrow mesenchymal stem cells (MSCs).


METHODS
Cell-scaffold complexes were implanted subcutaneously into the left back of the nude mice, and human natural bone derived material were implanted into the right back as control group. The mice were killed respectively on the postoperative 2 weeks, 4 weeks and 8 weeks. The macroscopic, histopathological, alkaline phosphatase (ALP) activity assay methods were performed to assess the ectopic osteogenesis potential.


RESULTS
The cartilaginous osteogenesis were observed in both deproteinated bone and decalcified bone, and the more new bone tissue formed gradually as the time went by after implantation. ALP activity become stronger followed with the time (P < 0.05), and compared with the decalcified bone, deproteinated bone displayed stronger ALP activity (P < 0.05).


CONCLUSIONS
The MSCs and human natural bone derived material can be used as good seed cells and scaffold materials respectively to construct tissue-engineered bone, and as the scaffold material, deproteinated bone has better osteogenesis ability than decalcifed bone.
Kang
Fei-Wu
FW
Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Tongji University, Shanghai 200072, China.
Tang
Xiu-Fa
XF
Wen
Yu-Ming
YM
Wu
Zheng-Hua
ZH
Huang
Xin
X
D000818:Animals; D001842:Bone and Bones; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells; D051379:Mice; D008819:Mice, Nude; D010012:Osteogenesis; D023822:Tissue Engineering