Comparative PCR-based restriction fragment length polymorphism analysis of the plasmid gene orf3 of Chlamydia trachomatis and Chlamydia psittaci.
The BfaI digestion of PCR-based restriction fragment length polymorphism analysis of the plasmid orf3 of Chlamydia trachomatis and Chlamydia psittaci provided evidence for two distinct restriction patterns, respectively. The nucleotide sequences of orf3 genes confirmed these differences. Serum antibodies against recombinant C. psittaci protein (pgp3) encoded by orf3 were detected both in pigeons with C. psittaci infection and in a human patient with psittacosis.
Storni
Elisa
E
Sezione di Microbiologia DMCSS, Policlinico S.Orsola, University of Bologna, Bologna, Italy.
Donati
Manuela
M
Marangoni
Antonella
A
Accardo
Silvia
S
Cevenini
Roberto
R
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000235:genetics*; D002691:Chlamydophila psittaci / Q000235:genetics*; D010856:Columbidae / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C082169:pgp3 protein, Chlamydia