title: Expression of tryptophan hydroxylase in developing mouse taste papillae.
abstract: Gustatory papillae and associated taste buds receive and process chemical information from the environment. In mammals, their development takes place during the late phase of embryogenesis. However, the cellular factors that regulate the differentiation of taste papillae remain largely unknown. Here, we show by quantitative real time RT-PCR that both isoforms of tryptophan hydroxylase (TPH1 and TPH2), the first and rate limiting enzyme of serotonin (5-HT) synthesis, are expressed in developing circumvallate papillae. Immuno-staining experiments further indicated that TPH is localized both in gustatory fibers and in differentiated taste receptor cells. These results point to the synthesis of 5-HT in gustatory papillae, and allow one to hypothesize that the development of taste buds might be modulated by serotonin.
authors.affiliation: UMR Physiologie des Adaptations Nutritionnelles, Institut National de la Recherche Agronomique-Université de Nantes, Rue de la Géraudière, B.P. 71627, 44316 Nantes, France.
authors.forename: Rafael
authors.initials: R
authors.lastname: Ortíz-Alvarado
authors.forename: Omar
authors.initials: O
authors.lastname: Guzmán-Quevedo
authors.forename: Rosalio
authors.initials: R
authors.lastname: Mercado-Camargo
authors.forename: Thomas
authors.initials: T
authors.lastname: Haertle
authors.forename: Caroline
authors.initials: C
authors.lastname: Vignes
authors.forename: Francisco
authors.initials: F
authors.lastname: Bolaños-Jiménez
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012701:Serotonin / Q000096:biosynthesis; D013650:Taste Buds / Q000166:cytology / Q000196:embryology* / Q000201:enzymology; D014365:Tryptophan Hydroxylase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D007527:Isoenzymes; D012701:Serotonin; C487801:Tph1 protein, mouse; C487802:Tph2 protein, mouse; D014365:Tryptophan Hydroxylase