Microarray detection of human parainfluenzavirus 4 infection associated with respiratory failure in an immunocompetent adult.
A pan-viral DNA microarray, the Virochip (University of California, San Francisco), was used to detect human parainfluenzavirus 4 (HPIV-4) infection in an immunocompetent adult presenting with a life-threatening acute respiratory illness. The virus was identified in an endotracheal aspirate specimen, and the microarray results were confirmed by specific polymerase chain reaction and serological analysis for HPIV-4. Conventional clinical laboratory testing using an extensive panel of microbiological tests failed to yield a diagnosis. This case suggests that the potential severity of disease caused by HPIV-4 in adults may be greater than previously appreciated and illustrates the clinical utility of a microarray for broad-based viral pathogen screening.
Chiu
Charles Y
CY
Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA.
Rouskin
Silvi
S
Koshy
Anita
A
Urisman
Anatoly
A
Fischer
Kael
K
Yagi
Shigeo
S
Schnurr
David
D
Eckburg
Paul B
PB
Tompkins
Lucy S
LS
Blackburn
Brian G
BG
Merker
Jason D
JD
Patterson
Bruce K
BK
Ganem
Don
D
DeRisi
Joseph L
JL
D000328:Adult; D001990:Bronchiolitis, Viral / Q000175:diagnosis; D005260:Female; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D029482:Parainfluenza Virus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D011024:Pneumonia, Viral / Q000175:diagnosis*; D019351:Rubulavirus Infections / Q000175:diagnosis* / Q000821:virology; D012698:Serologic Tests / Q000379:methods; D015898:Tomography Scanners, X-Ray Computed