[Jingjielianqiao Decoction promotes leg ulcer healing in rabbits].
OBJECTIVE
To study the effect of Jingjielianqiao Decoction in promoting leg ulcer in rabbits.


METHODS
Nine adult male New Zealand albino rabbits with chronic leg ulcers were randomized into 3 groups, namely group A treated with Jingjielianqiao Decoction, group B with Shengjiyuhong Decoction, and group C with normal saline. Gross observation of the wounds was carried out regularly for evaluating the changes in the ulcerous area, depth and wound surface excretion. After 3 weeks of treatment, the tissues on the edge of the ulcer were sampled and prepared for routine pathological examination, electron microscopy and immunohistochemistry for vascular endothelial growth factor (VEGF) and CD34. The number of blood vessels and their areas were also recorded.


RESULTS
The wounds showed no significant differences between the 3 groups by gross observation during the treatment, but after completion of the 3-week treatment, routine pathological examination and electron microscopy revealed significant differences between the groups. Immunohistochemistry for VEGF and CD34 yielded comparable results between groups A and B (positive control), but showed significant differences between group C and the other two groups (P<0.01).


CONCLUSIONS
Jingjielianqiao Decoction and Shengjiyuhong Decoction can obviously promote the healing of leg ulcer in rabbits.
Lan
Hai-mei
HM
Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Liu
Xue-qing
XQ
Chen
Bao-tian
BT
Yang
Liu
L
Qng
Jing
J
Lai
Mei-sheng
MS
D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D007150:Immunohistochemistry; D007871:Leg Ulcer / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D008517:Phytotherapy; D011817:Rabbits; D011897:Random Allocation; D012867:Skin / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis; D014945:Wound Healing / Q000187:drug effects*
D018952:Antigens, CD34; D004365:Drugs, Chinese Herbal; D042461:Vascular Endothelial Growth Factor A; C529137:jingjielianqiao