[Antigen-antibody reaction model of solid-phase surface and active biochip system].
To overcome the present limitations of passive biochip, based on the basic principle of antigen-antibody reaction, we develop an antigen-antibody reaction model of solid-phase surface and design a novel active biochip system according to this model, which introduces the negative pressure and controlling devices to control the immunoreactions on the nitrocellulose (NC) membrane. From the computer simulation results, this is a rapid, stable, robust and practicable system, which can be used to increase the efficiency of immunoreactions and improve the reproducibility and accuracy of biochip analysis.
Zhu
Wenwei
W
Institute of Genetic Diagnosis, The Fourth Military Medical University, Xi'an 710032, China. zhuwenwei428@hotmail.com
Zhang
Wenhong
W
Zhu
Wenbiao
W
Han
Fengchan
F
Dong
Xiuzhen
X
Yan
Xiaojun
X
D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D008954:Models, Biological