The role of a heat shock protein from V. cholerae 0139 in the gut immune response.
An immunodominant heat shock protein (Hsp 24) was purified from Vibrio cholerae O139 at 42 degrees C and used as an immunomodulator for studying the gut immune response. T cell clone and T cell line specific for the Hsp 24 were generated from the lymphocytes of lamina propria and intra-epithelial lymphocytes of mice orally infected with V. cholerae O139, respectively. The T cell clone was TCR alphabeta(+), CD4(+) and appeared to play an important role in the functioning of gut B-lymphocytes. The T cell line had heterogenous population of CD8+ and CD4+ cells, most of which were found to be TCR alphabeta(+) and a minor population was TCR gammadelta(+). The lymphokine profile of T cell line showed IFN-gamma to be the most abundant lymphokine followed by IL-2 and IL-4. The possible involvement of alternative pathway of activation for T cell clone was also addressed in this study. The splenocytes showed an up-regulation of their CD2 receptor expression on stimulation with the Hsp-24. The pattern of lymphokines released by splenocytes stimulated with the Hsp-24 showed no particular cell type to be responsible for mounting immune response. Thus, there is involvement of both, mucosal and peripheral arm of the immune system.
Mazumdar
Shibnath
S
Department of Experimental Medicine and Biotechnology, PGIMER, Chandigarh 160012, India. shalmoli@glide.net.in
Bhattacharyya
Shalmoli
S
Ghosh
Sujata
S
Majumdar
Siddhartha
S
Ganguly
Nirmal K
NK
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology; D001426:Bacterial Proteins / Q000276:immunology*; D049109:Cell Proliferation; D002771:Cholera; D002999:Clone Cells; D018920:Coculture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D041981:Gastrointestinal Tract / Q000276:immunology* / Q000382:microbiology*; D006360:Heat-Shock Proteins / Q000276:immunology*; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008222:Lymphokines / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology; D041242:Vibrio cholerae O139 / Q000276:immunology* / Q000378:metabolism
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D006360:Heat-Shock Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D008222:Lymphokines; D011948:Receptors, Antigen, T-Cell