title: Physiological indicators of cell function.
abstract: Successful high content screening (HCS) assays place large demands on the cell-based reagents used in their development and deployment. Fortunately, there is a wide range of fluorescent physiological indicators from which to choose that are continually increasing in size and variety. Ideal fluorescent reagents for cell-based assays exhibit optimal selectivity, signal intensity, and cell solubility, yet will be easily incorporated into assays across multiple detection platforms. The repertoire of existing fluorogenic and color changing dyes that indicate physiological changes in cells for live cell kinetic and fixed end-point assays are surveyed as well as newly developed reagents for the next generation of HCS assays.
authors.affiliation: Molecular Probes/Invitrogen, Eugene, OR, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Ignatius
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: Hung
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D015374:Biosensing Techniques; D002118:Calcium / Q000378:metabolism; D002477:Cells / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D015335:Molecular Probes / Q000032:analysis*; D017785:Photons; D011188:Potassium / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
chemical_list: D000906:Antibodies; D007202:Indicators and Reagents; D015335:Molecular Probes; D011506:Proteins; D012964:Sodium; D011188:Potassium; D002118:Calcium