title: The effect of reuterin on the lag time of single cells of Listeria innocua grown on a solid agar surface at different pH and NaCl concentrations.
abstract: The lag time of single cells of Listeria innocua grown on the surface of Brain Heart Infusion Agar was studied by microscopy and image analysis. An experimental set-up that enabled relocation of the cells on the agar surface was developed and used to collect data from 50 to 100 individual cells at a time. Reuterin was added at different concentrations (0-10 AU/ml) and it was observed that it increased both the lag time of the cells and its variance. Furthermore, for a large proportion of cells, reuterin completely prevented the cell division within the time of observation. Reuterin in combination with low pH inhibited the cell division even more efficiently. A similar effect was observed for the combination of reuterin and sodium chloride. Our experimental set-up provides a good model system for generating data on the lag time of single cells on solid surfaces, which can improve the predictions of microbial growth on solid food matrices.
authors.affiliation: Department of Food Science, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark.
authors.forename: Maria
authors.initials: M
authors.lastname: Rasch
authors.forename: Aline
authors.initials: A
authors.lastname: Métris
authors.forename: József
authors.initials: J
authors.lastname: Baranyi
authors.forename: Birgitte
authors.initials: B
authors.lastname: Bjørn Budde
mesh_terms: D000362:Agar / Q000737:chemistry; D000447:Aldehydes / Q000494:pharmacology*; D018914:Cells, Immobilized; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D005985:Glyceraldehyde / Q000031:analogs & derivatives* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008087:Listeria / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D011407:Propane / Q000494:pharmacology*; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D000447:Aldehydes; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; D012965:Sodium Chloride; D000362:Agar; D011407:Propane