Characterization of 75:25 poly(l-lactide-co-epsilon-caprolactone) thin films for the endoluminal delivery of adipose-derived stem cells to abdominal aortic aneurysms.
Abdominal aortic aneurysms occur in 5-7% of men over the age of 60 and their incidence is rising. Current therapies remove the affected tissue or prevent blood flow through the aneurysm, but do not repair the underlying structural changes of the vascular wall. Adipose tissue derived stem cells (ADSCs) seeded on a biodegradable thin film and delivered endoluminally to the aneurysm site could potentially repair the vessel wall, preventing growth and rupture of the aneurysm. In this study, the mechanical and degradation properties of a novel 75:25 poly(l-lactide-co-epsilon-caprolactone) (PLCL) thin film, as well as, the effects of different surface structures on stem cell adherence and resistance to shear stress was investigated. It was possible to reproducibly create films of consistent physical properties. These films degraded approximately 50% in 6 month, which would be a sufficient time to allow cells to engraft in the aortic wall. Ethylene oxide treatment significantly increased the stiffness and yield stress of the films, which exhibit >700% elongation. Treatment of the films with NaOH and HCl induced the formation of surface texture on the films; however, this texture did not affect stem cell adherence or resistance to delamination by shear stress when compared to nontreated or fibronectin-coated films. These results indicate that PLCL thin films have a sufficient degradation time and mechanical strength to serve as a scaffold in vivo for ADSCs, and that ADSCs seeded on the thin film can withstand a range of physiologic shear stresses.
Burks
Chris A
CA
Section of Cardiology, Department of Medicine, Tulane University Health Science Center, New Orleans, Louisiana , USA.
Bundy
Kirk
K
Fotuhi
Parwis
P
Alt
Eckhard
E
D000273:Adipose Tissue / Q000166:cytology* / Q000637:transplantation; D017544:Aortic Aneurysm, Abdominal / Q000628:therapy*; D001672:Biocompatible Materials; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D008422:Materials Testing / Q000379:methods; D008875:Middle Aged; D011091:Polyesters; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000166:cytology*; D013718:Tensile Strength
D001672:Biocompatible Materials; D011091:Polyesters; C062968:lactide-caprolactone copolymer