Antidepressant principles of the roots of Polygala tenuifolia.
[(125)I]RTI-55-membrane binding assay-guided fractionation and separation of a water-soluble extract of the roots of Polygala tenuifolia gave five new oligosaccharide derivatives, polygalatenosides A-E (1-5). The structures of these new oligosaccharides were established on the basis of spectroscopic evidence. Polygalatenosides A and B (1 and 2) showed significant inhibitory activity, with IC(50) values of 30.0 and 6.04 microM, respectively, in this membrane binding assay and acted as norepinephrine reuptake inhibitors through blocking norepinephrine transport.
Cheng
Mo-Chi
MC
Medical and Pharmaceutical Industry Technology and Development Center, Taipei County, Taiwan, Republic of China.
Li
Chia-Ying
CY
Ko
Han-Chieh
HC
Ko
Feng-Nien
FN
Lin
Yun-Lian
YL
Wu
Tian-Shung
TS
D000928:Antidepressive Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D003042:Cocaine / Q000031:analogs & derivatives / Q000494:pharmacology; D003891:Desipramine / Q000494:pharmacology; D015394:Molecular Structure; D050484:Norepinephrine Plasma Membrane Transport Proteins / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D031801:Polygala / Q000737:chemistry*; D013624:Taiwan
D000928:Antidepressive Agents; D050484:Norepinephrine Plasma Membrane Transport Proteins; D009844:Oligosaccharides; C069723:2beta-carbomethoxy-3beta-(4-iodophenyl)tropane; D003042:Cocaine; D003891:Desipramine