[Effect of Pranlukast on tissue remodeling in experimental guinea pig allergic rhinitis model].
OBJECTIVE
To explore the impact of Pranlukast in nasal mucosal remodeling in experimental allergic rhinitis.


METHODS
Fourteen male guinea pigs were randomly divided into 3 groups: control group, ovalbumin (OVA) group and OVA + Pranlukast group. In the OVA group and OVA + Pranlukast group, OVA sensitized Hartley guinea pigs were exposured intranasally to OVA for a total of 12 weeks, the OVA + Pranlukast group received additional Pranlukast treatment from the second week to the 12th week. Paraffin embedded sections were stained with hematoxylin and eosin (HE), alcian blue-periodic acid-Schiff (AB-PAS), and Masson's Trichrome (MT). Infiltrating eosinophils, the number of goblet cells in the surface epithelium and gland cells in subepithelial nasal septal mucosa were counted. The damage of epithelium in nasal septum and extracellular matrix of nasal septal mucosa and conchae were determined.


RESULTS
Compared with the control, the prolonged OVA exposure protocol caused significant pathological changes in the nasal mucosa, which included eosinophils infiltration into epithelium and submucosa (106.90 +/- 13.66), significant goblet hyperplasia (22.05 +/- 5.81/mm), epithelial damage (intact epithelium: 47.25% +/- 7.67%) and deposition of extracellular matrix. These changes were significantly inhibited by Pranlukast, in which group, there were few eosinophils(8.95 +/- 2.32) , few goblet cells (5.73 +/- 1.07/mm), and relative intact epithelium (intact epithelium: 83.15% +/- 8.05%), and no significant ECM deposition.


CONCLUSIONS
Early Pranlukast intervention could inhibit nasal mucosal remodeling in allergic rhinitis.
She
Wen-Yu
WY
Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Union Hospital Jilin University, Changchun 130033, China.
Dong
Zhen
Z
D000818:Animals; D002867:Chromones / Q000494:pharmacology*; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D006168:Guinea Pigs; D008297:Male; D009297:Nasal Mucosa / Q000187:drug effects* / Q000473:pathology*; D012220:Rhinitis / Q000473:pathology*; D012221:Rhinitis, Allergic, Perennial / Q000473:pathology
D002867:Chromones; C047681:pranlukast