title: Transplantation of an autologous mesothelial cell sheet prepared from tunica vaginalis prevents post-operative adhesions in a canine model.
abstract: Post-operative adhesions often cause severe complications such as bowel obstruction and abdominopelvic pain. Previously, we reported that transplantation of a mesothelial cell sheet is effective for preventing adhesion in rat model. We also proposed a new technique for harvesting autologous mesothelial cells from tunica vaginalis without intra-abdominal maneuvers. In this study, we examined whether an autologous mesothelial cell sheet can prevent post-operative peritoneal adhesions in a canine adhesion model. Mesothelial cells were isolated from the tunica vaginalis of male beagles. Isolated cells were cultured on fibrin gel. We named this construct the "mesothelial cell sheet." Animals underwent surgery to induce peritoneal adhesion formation and were then transplanted with the mesothelial cell sheets (sheet group, n = 4), fibrin gel (fibrin group, n = 4), or no materials (sham group, n = 4). Four weeks after the transplantation, we evaluated adhesion formation and scored adhesion levels. The abdominal wall transplanted with the mesothelial cell sheet was covered with mesothelium. The total adhesion score of the sheet group was significantly lower than that of the fibrin group and the sham group. These results indicated that transplantation of an autologous mesothelial cell sheet is effective for preventing post-operative adhesion formation in the canine adhesion model. Our mesothelial cell sheet has the potential to be a powerful adhesion prophylactic material in surgery.
authors.affiliation: Department of Urology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Touko
authors.initials: T
authors.lastname: Asano
authors.forename: Ryoji
authors.initials: R
authors.lastname: Takazawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Ryo
authors.initials: R
authors.lastname: Takagi
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Iimura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Masuda
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kihara
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D034861:Abdominal Wall / Q000473:pathology; D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004285:Dogs; D004847:Epithelial Cells / Q000637:transplantation* / Q000648:ultrastructure; D004848:Epithelium / Q000637:transplantation / Q000648:ultrastructure; D005260:Female; D008297:Male; D051381:Rats; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control*; D014182:Transplantation, Autologous; D014621:Vagina / Q000648:ultrastructure