title: The Connectivity Map: using gene-expression signatures to connect small molecules, genes, and disease.
abstract: To pursue a systematic approach to the discovery of functional connections among diseases, genetic perturbation, and drug action, we have created the first installment of a reference collection of gene-expression profiles from cultured human cells treated with bioactive small molecules, together with pattern-matching software to mine these data. We demonstrate that this "Connectivity Map" resource can be used to find connections among small molecules sharing a mechanism of action, chemicals and physiological processes, and diseases and drugs. These results indicate the feasibility of the approach and suggest the value of a large-scale community Connectivity Map project.
authors.affiliation: Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge, MA 02142, USA. justin@broad.mit.edu
authors.forename: Justin
authors.initials: J
authors.lastname: Lamb
authors.forename: Emily D
authors.initials: ED
authors.lastname: Crawford
authors.forename: David
authors.initials: D
authors.lastname: Peck
authors.forename: Joshua W
authors.initials: JW
authors.lastname: Modell
authors.forename: Irene C
authors.initials: IC
authors.lastname: Blat
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Wrobel
authors.forename: Jim
authors.initials: J
authors.lastname: Lerner
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Brunet
authors.forename: Aravind
authors.initials: A
authors.lastname: Subramanian
authors.forename: Kenneth N
authors.initials: KN
authors.lastname: Ross
authors.forename: Michael
authors.initials: M
authors.lastname: Reich
authors.forename: Haley
authors.initials: H
authors.lastname: Hieronymus
authors.forename: Guo
authors.initials: G
authors.lastname: Wei
authors.forename: Scott A
authors.initials: SA
authors.lastname: Armstrong
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Haggarty
authors.forename: Paul A
authors.initials: PA
authors.lastname: Clemons
authors.forename: Ru
authors.initials: R
authors.lastname: Wei
authors.forename: Steven A
authors.initials: SA
authors.lastname: Carr
authors.forename: Eric S
authors.initials: ES
authors.lastname: Lander
authors.forename: Todd R
authors.initials: TR
authors.lastname: Golub
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy / Q000235:genetics; D002460:Cell Line; D045744:Cell Line, Tumor; D016208:Databases, Factual; D003907:Dexamethasone / Q000494:pharmacology / Q000627:therapeutic use; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D019008:Drug Resistance, Neoplasm; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors; D056572:Histone Deacetylase Inhibitors; D006801:Humans; D036701:Limonins / Q000494:pharmacology; D009765:Obesity / Q000235:genetics / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis; D010640:Phenothiazines / Q000494:pharmacology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy / Q000235:genetics / Q000503:physiopathology; D020123:Sirolimus / Q000494:pharmacology / Q000627:therapeutic use; D012984:Software
chemical_list: D004791:Enzyme Inhibitors; D004967:Estrogens; D018841:HSP90 Heat-Shock Proteins; D056572:Histone Deacetylase Inhibitors; D036701:Limonins; D010640:Phenothiazines; C106014:gedunin; D003907:Dexamethasone; D020123:Sirolimus