Development of a real-time PCR assay for detection of gyrA mutations associated with reduced susceptibility to ciprofloxacin in Salmonella enterica serovar typhi and paratyphi A.
A real-time PCR assay with the cycling probe method was used to detect mutations at codons 83 and 87 in the DNA gyrase A subunit encoded by gyrA in Salmonella enterica serovar Typhi and Paratyphi A clinical isolates. The susceptibility estimated from the results of the gyrA mutation assay was consistent with that identified by the culture method using an E-test. This assay allows rapid screening of S. enterica serovar Typhi and Paratyphi A with reduced susceptibility to ciprofloxacin.
Morita
Masatomo
M
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan.
Ito
Kenitiro
K
Hirose
Kenji
K
Takahashi
Hideyuki
H
Shimuta
Ken
K
Terajima
Jun
J
Ohnishi
Makoto
M
Harada
Makoto
M
Matsuzaki
Mitsuhiro
M
Watanabe
Haruo
H
Izumiya
Hidemasa
H
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D006801:Humans; D009154:Mutation; D010284:Paratyphoid Fever / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012482:Salmonella paratyphi A / Q000187:drug effects* / Q000235:genetics; D012485:Salmonella typhi / Q000187:drug effects* / Q000235:genetics; D014435:Typhoid Fever / Q000382:microbiology
D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D027081:DNA Gyrase