title: Expression profiling characterization of laminin alpha-2 positive MDC.
abstract: In the Caucasian population, patients affected by the most frequent forms of congenital muscular dystrophies (MDC) are commonly divided into two groups. The first is characterized by mutations of the gene for the laminin alpha-2 (LAMA2). The second is positive for this protein, highly heterogeneous, and has no specific genetic defect associated yet. We studied the skeletal muscle transcriptome of four LAMA2 deficient and six LAMA2 positive MDC patients by cDNA microarrays. The expression profiling defined two patients groups: one mild and one severe phenotype. This result was in agreement with histopathological features but only partially with the clinical classification. The mild phenotype is characterized by a delayed maturation from slow to fast muscle fibers. Other muscle transcripts, such as telethonin, myosin light-chains 3 and 1V, are underexpressed in this group. We suggest that expression profiling will provide important information to improve our understanding of the molecular basis of laminin alpha-2 positive MDC.
authors.affiliation: CRIBI Biotechnology Center and Dipartimento di Biologia, Università degli Studi di Padova, Padova, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Millino
authors.forename: Milena
authors.initials: M
authors.lastname: Bellin
authors.forename: Marina
authors.initials: M
authors.lastname: Fanin
authors.forename: Chiara
authors.initials: C
authors.lastname: Romualdi
authors.forename: Elena
authors.initials: E
authors.lastname: Pegoraro
authors.forename: Corrado
authors.initials: C
authors.lastname: Angelini
authors.forename: Gerolamo
authors.initials: G
authors.lastname: Lanfranchi
mesh_terms: D003402:Creatine Kinase / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007797:Laminin / Q000032:analysis* / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D009136:Muscular Dystrophies / Q000151:congenital* / Q000235:genetics* / Q000378:metabolism
chemical_list: D007797:Laminin; C102645:laminin alpha 2; D003402:Creatine Kinase