title: Contribution of FliC to epithelial cell invasion by enterohemorrhagic Escherichia coli O113:H21.
abstract: Enterohemorrhagic Escherichia coli (EHEC) O113:H21 can invade epithelial cells. In this study, we found that invasion but not adherence was inhibited by anti-FliC(H21) specific antibodies. In addition, deletion of fliC(H21) from EHEC O113:H21 resulted in an eightfold decrease in invasion that was restored upon transcomplementation with fliC(H21) but not with fliC(H6). These results suggested that FliC plays an important role in the pathogenesis of infections caused by EHEC O113:H21 by allowing bacteria to penetrate the intestinal epithelium.
authors.affiliation: Department of Microbiology, Monash University, Clayton 3800, Victoria, Australia.
authors.forename: Shelley N
authors.initials: SN
authors.lastname: Luck
authors.forename: Luminita
authors.initials: L
authors.lastname: Badea
authors.forename: Vicki
authors.initials: V
authors.lastname: Bennett-Wood
authors.forename: Roy
authors.initials: R
authors.lastname: Robins-Browne
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Hartland
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology*; D005408:Flagellin; D006801:Humans; D008969:Molecular Sequence Data; D017384:Sequence Deletion
chemical_list: D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; D005408:Flagellin