Carotid arterial stiffness, elastic fibre network and vasoreactivity in semicarbazide-sensitive amine-oxidase null mouse.
OBJECTIVE
We examined the arterial phenotype of semicarbazide-sensitive amine-oxidase null mouse (SSAO -/-) using various techniques including high resolution echotracking.


RESULTS
SSAO -/- mice showed no change in arterial pressure under anesthesia. The in vivo arterial diameter, only measured in the carotid artery (CA), was higher in SSAO -/- than in SSAO +/+ animals. Elastic modulus-wall stress curves and CA rupture pressure were similar between SSAO -/- and +/+ mice, indicating no change in arterial wall stiffness or mechanical strength. There was no significant difference in insoluble elastin, total collagen content and elastic lamellar morphology between the two genotypes. No alteration in vascular reactivity was observed in aortic rings and mesenteric arteries from SSAO -/- mice. Aortic lysyl oxidase (LO) activity remained unaltered, indicating that SSAO invalidation is not accompanied by a compensatory increase in LO activity.


CONCLUSIONS
This is the first functional study of arteries lacking SSAO. Our results indicate that SSAO -/- mice present an increased arterial diameter associated with normal arterial mechanical properties, suggesting that SSAO deficiency might contribute to arterial wall remodeling. However, these results argue against the hypothesis that SSAO intervenes in elastic fibre organization, elastin cross-linking processes and vasoreactivity.
Mercier
Nathalie
N
INSERM U684, Vandoeuvre-les-Nancy, France.
Osborne-Pellegrin
Mary
M
El Hadri
Khadija
K
Kakou
Augustine
A
Labat
Carlos
C
Loufrani
Laurent
L
Henrion
Daniel
D
Challande
Pascal
P
Jalkanen
Sirpa
S
Fève
Bruno
B
Lacolley
Patrick
P
D006631:Amine Oxidase (Copper-Containing) / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001011:Aorta; D015153:Blotting, Western / Q000379:methods; D017536:Carotid Artery, Common / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D003094:Collagen / Q000032:analysis; D004547:Elastic Tissue / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D004548:Elasticity; D004549:Elastin / Q000032:analysis; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008297:Male; D008638:Mesenteric Arteries / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D008249:Protein-Lysine 6-Oxidase / Q000032:analysis; D033081:Shear Strength; D014666:Vasomotor System / Q000502:physiology
D003094:Collagen; D004549:Elastin; D008249:Protein-Lysine 6-Oxidase; D006631:Amine Oxidase (Copper-Containing)