Monopolar attachment of sister kinetochores at meiosis I requires casein kinase 1.
In meiosis, a single round of DNA replication is followed by two consecutive rounds of chromosome segregation, called meiosis I and II. Disjunction of maternal from paternal centromeres during meiosis I depends on the attachment of sister kinetochores to microtubules emanating from the same pole. In budding yeast, monopolar attachment requires recruitment to kinetochores of the monopolin complex. How monopolin promotes monopolar attachment was unclear, as its subunits are poorly conserved and lack similarities to proteins with known functions. We show here that the monopolin subunit Mam1 binds tightly to Hrr25, a highly conserved casein kinase 1 delta/epsilon (CK1delta/epsilon), and recruits it to meiosis I centromeres. Hrr25 kinase activity and Mam1 binding are both essential for monopolar attachment. Since CK1delta/epsilon activity is important for accurate chromosome segregation during meiosis I also in fission yeast, phosphorylation of kinetochore proteins by CK1delta/epsilon might be an evolutionary conserved process required for monopolar attachment.
Petronczki
Mark
M
Research Institute of Molecular Pathology, Dr. Bohrgasse 7, A-1030 Vienna, Austria.
Matos
Joao
J
Mori
Saori
S
Gregan
Juraj
J
Bogdanova
Aliona
A
Schwickart
Martin
M
Mechtler
Karl
K
Shirahige
Katsuhiko
K
Zachariae
Wolfgang
W
Nasmyth
Kim
K
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D047389:Casein Kinase I / Q000235:genetics / Q000378:metabolism*; D002503:Centromere / Q000235:genetics / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D020090:Chromosome Segregation / Q000502:physiology*; D004261:DNA Replication / Q000502:physiology; D018386:Kinetochores / Q000378:metabolism* / Q000648:ultrastructure; D008540:Meiosis / Q000502:physiology*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
D018528:ATP-Binding Cassette Transporters; D002868:Chromosomal Proteins, Non-Histone; D021122:Protein Subunits; C502898:REC8 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C107004:mam1 protein, S pombe; D047389:Casein Kinase I; C070775:HRR25 protein, S cerevisiae