title: Single nucleotide polymorphism in the cytolethal distending toxin B gene confers heterogeneity in the cytotoxicity of Actinobacillus actinomycetemcomitans.
abstract: Clinical Actinobacillus actinomycetemcomitans produces cytolethal distending toxin (CDT) with titers ranging from 10(2) to 10(8) U/mg. Single nucleotide polymorphism analysis of the cdt gene in clinical isolates identified a variation of a single amino acid at residue 281 of CdtB, which significantly affected CDT toxicity by modulating the chromatin-degrading activity of CdtB.
authors.affiliation: Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8553, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nishikubo
authors.forename: Masaru
authors.initials: M
authors.lastname: Ohara
authors.forename: Masae
authors.initials: M
authors.lastname: Ikura
authors.forename: Katsuo
authors.initials: K
authors.lastname: Katayanagi
authors.forename: Tamaki
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Komatsuzawa
authors.forename: Hidemi
authors.initials: H
authors.lastname: Kurihara
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Sugai
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001427:Bacterial Toxins / Q000235:genetics* / Q000633:toxicity*; D002843:Chromatin / Q000187:drug effects; D005798:Genes, Bacterial; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D020641:Polymorphism, Single Nucleotide; D037521:Virulence Factors / Q000235:genetics* / Q000633:toxicity*
chemical_list: D001427:Bacterial Toxins; D002843:Chromatin; D037521:Virulence Factors; C067044:cytolethal distending toxin