Cell density- and ComE-dependent expression of a group of mutacin and mutacin-like genes in Streptococcus mutans.
Streptococcus mutans is a major cariogenic inhabitant of the high cell density oral biofilm (dental plaque). In previous studies, we showed that production of one of its virulence factors, the bacteriocin mutacin IV, was regulated by high cell density as well as the competence regulatory system ComED. In this study, we utilized luciferase fusions and real-time reverse transcriptase polymerase chain reaction (RT-PCR), to demonstrate that high cell density and ComED also regulate an uncharacterized group of mutacin and mutacin-like genes. Under high cell density or in the presence of externally added competence-stimulating peptide (CSP), gene expression increased 10- to 30-fold. Interestingly, high cell density was able to bypass the requirement for CSP addition. However, both cell density and CSP-dependent gene expression had a strict requirement for the ComE response regulator.
Kreth
Jens
J
Department of Oral Biology and Medicine, UCLA School of Dentistry, University of California-Los Angeles, Los Angeles, CA 90095, USA.
Merritt
Justin
J
Zhu
Lin
L
Shi
Wenyuan
W
Qi
Fengxia
F
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003731:Dental Caries / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D013295:Streptococcus mutans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D001426:Bacterial Proteins; D001430:Bacteriocins; C103179:ComE protein, Streptococcus pneumoniae; C423628:competence factor, Streptococcus; C425769:mutacin IV