SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects.
This paper reviews the developments in high-throughput and nanolitre-scale protein crystallography technologies within the remit of workpackage 4 of the Structural Proteomics In Europe (SPINE) project since the project's inception in October 2002. By surveying the uptake, use and experience of new technologies by SPINE partners across Europe, a picture emerges of highly successful adoption of novel working methods revolutionizing this area of structural biology. Finally, a forward view is taken of how crystallization methodologies may develop in the future.
Berry
Ian M
IM
Division of Structural Biology and Oxford Protein Production Facility (OPPF), Wellcome Trust Centre for Human Genetics, Oxford, England.
Dym
O
O
Esnouf
R M
RM
Harlos
K
K
Meged
R
R
Perrakis
A
A
Sussman
J L
JL
Walter
T S
TS
Wilson
J
J
Messerschmidt
Albrecht
A
D003461:Crystallography / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D007091:Image Processing, Computer-Assisted; D036103:Nanotechnology; D010969:Plastics; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics; D011786:Quality Control; D012371:Robotics
D010969:Plastics; D011506:Proteins