title: Differences in the growth of paired Ugandan isolates of Mycobacterium tuberculosis within human mononuclear phagocytes correlate with epidemiological evidence of strain virulence.
abstract: Previous studies have suggested that isolates of Mycobacterium tuberculosis responsible for tuberculosis outbreaks grow more rapidly within human mononuclear phagocytes than do other isolates. Clinical scenarios suggesting virulence of specific M. tuberculosis isolates are readily identified. Determination of appropriate "control" isolates for these studies is more problematic, but equally important for validating these assays and, ultimately, for identifying biologic differences between M. tuberculosis strains that contribute to virulence. We utilized the database from a study of Ugandan tuberculosis patients and their household (HH) contacts to identify M. tuberculosis isolates transmitted within HH and nontransmitted control isolates. Isolate pairs were evaluated from matched HH in each of three clinical scenarios: (i) coprevalent disease and no disease, (ii) incident disease and no disease, and (iii) M. tuberculosis infection (purified protein derivative [PPD] positive) and no infection (PPD negative). Intracellular growth of paired organisms was determined in a blinded fashion using two models of intracellular infection in which we have previously demonstrated correlation between intracellular growth and strain virulence, primary human monocytes (MN) and THP-1 human macrophage-like cells. In both models, transmitted isolates from coprevalent disease HH displayed more rapid growth than nontransmitted control isolates. In the THP-1 model, this was also true of transmitted isolates from HH with incident disease and their controls. Differences in production of tumor necrosis factor alpha and interleukin-10 by matched isolates showed correlation with growth patterns in the THP-1 cells but not in MN. Paired isolates characterized in this manner may be of particular interest for further investigations of the virulence of M. tuberculosis.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Biomedical Research Building, Rm. 1030, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44122, USA.
authors.forename: Sue A
authors.initials: SA
authors.lastname: Theus
authors.forename: M Donald
authors.initials: MD
authors.lastname: Cave
authors.forename: Kathleen
authors.initials: K
authors.lastname: Eisenach
authors.forename: Jessica
authors.initials: J
authors.lastname: Walrath
authors.forename: Hung
authors.initials: H
authors.lastname: Lee
authors.forename: Wilma
authors.initials: W
authors.lastname: Mackay
authors.forename: Christopher
authors.initials: C
authors.lastname: Whalen
authors.forename: Richard F
authors.initials: RF
authors.lastname: Silver
mesh_terms: D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D005191:Family Characteristics; D006801:Humans; D009000:Monocytes / Q000276:immunology / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D010586:Phagocytes / Q000276:immunology* / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000453:epidemiology* / Q000276:immunology / Q000635:transmission; D014454:Uganda / Q000453:epidemiology; D014774:Virulence
chemical_list: D016207:Cytokines