Phenylalanine dehydrogenase mutants: efficient biocatalysts for synthesis of non-natural phenylalanine derivatives.
Wild-type phenylalanine dehydrogenase from Bacillus sphaericus, and three mutants N145A, N145V and N145L, are used with a coenzyme recycling system to synthesise L-phenylalanine and three non-natural amino acids (p-F-phenylalanine, p-MeO-phenylalanine and p-CF(3)-phenylalanine) on a millimole scale. A range of reaction conditions are investigated. The kinetic parameters of WT PheDH and N145A towards p-CF(3)-phenylpyruvate are compared, emphasising the value of protein engineering in creating improved biocatalysts.
Paradisi
Francesca
F
UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.
Collins
Stuart
S
Maguire
Anita R
AR
Engel
Paul C
PC
D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D001711:Biotransformation; D007700:Kinetics; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D010649:Phenylalanine / Q000096:biosynthesis*; D013237:Stereoisomerism
D010649:Phenylalanine; D000594:Amino Acid Oxidoreductases; C022342:phenylalanine oxidase