title: The vesicle transport protein Vac1p is required for virulence of Candida albicans.
abstract: The putative vesicle transport protein Vac1p of the human pathogenic yeast Candida albicans plays an important role in virulence. To determine the cellular functions of Vac1p, a null mutant was generated by sequential disruption of both alleles. The vac1 null mutant strain showed defective endosomal vesicle transport, demonstrating a role of Vac1p in protein transport to the vacuole. Vac1p also contributes to resistance to metal ions, as the null mutant strain was hypersensitive to Cu(2+), Zn(2+) and Ni(2+). In addition, the loss of Vac1p affected several virulence factors of C. albicans. In particular, the vac1 null mutant strain showed defective hyphal growth, even when hyphal formation was induced via different pathways. Furthermore, Vac1p affects chlamydospore formation, adherence to human vaginal epithelial cells, and the secretion of aspartyl proteinases (Saps). Avirulence in a mouse model of systemic infection of the vac1 null mutant strongly suggests that Vac1p of C. albicans is essential for pathogenicity. In summary, the Vac1p protein is required for several cellular pathways, in particular those that control virulence and pathogenicity. Consequently, Vac1p is a novel and interesting target for antifungal drugs.
authors.affiliation: University of Applied Sciences, Department of Medical Engineering, Carl-Zeiss-Promenade 2, D-07745 Jena, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Franke
authors.affiliation: Leibniz-Institute for Natural Products Research and Infection Biology/Hans-Knöll-Institute, Department of Infection Biology, Beutenbergstrasse 11, D-07745 Jena, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Nguyen
authors.affiliation: Leibniz-Institute for Natural Products Research and Infection Biology/Hans-Knöll-Institute, Department of Infection Biology, Beutenbergstrasse 11, D-07745 Jena, Germany.
authors.forename: Albert
authors.initials: A
authors.lastname: Härtl
authors.affiliation: Leibniz-Institute for Natural Products Research and Infection Biology/Hans-Knöll-Institute, Department of Infection Biology, Beutenbergstrasse 11, D-07745 Jena, Germany.
authors.forename: Hans-Martin
authors.initials: HM
authors.lastname: Dahse
authors.affiliation: Department of Hygiene, Microbiology and Social Medicine, Fritz-Pregl-Strasse 3, A-6020 Innsbruck, Austria.
authors.forename: Georgia
authors.initials: G
authors.lastname: Vogl
authors.affiliation: Department of Hygiene, Microbiology and Social Medicine, Fritz-Pregl-Strasse 3, A-6020 Innsbruck, Austria.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Würzner
authors.affiliation: Leibniz-Institute for Natural Products Research and Infection Biology/Hans-Knöll-Institute, Department of Infection Biology, Beutenbergstrasse 11, D-07745 Jena, Germany.
authors.forename: Peter F
authors.initials: PF
authors.lastname: Zipfel
authors.affiliation: University of Applied Sciences, Department of Medical Engineering, Carl-Zeiss-Promenade 2, D-07745 Jena, Germany.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Künkel
authors.affiliation: University of Applied Sciences, Department of Medical Engineering, Carl-Zeiss-Promenade 2, D-07745 Jena, Germany.
authors.forename: Raimund
authors.initials: R
authors.lastname: Eck
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity*; D002177:Candidiasis; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D003300:Copper / Q000494:pharmacology; D004195:Disease Models, Animal; D017353:Gene Deletion; D006801:Humans; D025301:Hyphae / Q000254:growth & development; D051379:Mice; D008853:Microscopy; D008856:Microscopy, Fluorescence; D009024:Morphogenesis / Q000235:genetics; D009532:Nickel / Q000494:pharmacology; D013172:Spores, Fungal; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000502:physiology*; D014774:Virulence / Q000235:genetics; D015032:Zinc / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D033921:Vesicular Transport Proteins; D003300:Copper; D009532:Nickel; D016282:Aspartic Acid Endopeptidases; D015032:Zinc