title: Primary testicular diffuse large B-cell lymphoma belongs to the nongerminal center B-cell-like subgroup: A study of 18 cases.
abstract: The most common type of primary testicular lymphoma is diffuse large B-cell type, which has the potential for aggressive clinical behavior. Diffuse large B-cell lymphoma can be further subclassified into two major prognostic categories: germinal center B-cell-like and nongerminal center B-cell-like. Such distinction is made possible using the immunohistochemical expression of CD10, Bcl-6 and MUM1. The aim of this study was to stratify primary testicular lymphoma of the diffuse large B-cell type according to this scheme. Immunohistochemical stains for CD10, Bcl-6 and MUM1 were performed on 18 cases of primary testicular lymphoma of diffuse large B-cell type. Subclassification was carried out as previously described where CD10 and/or Bcl-6 positivity and negativity for MUM1 were considered indicative of germinal center B-cell-like type and the opposite expression as nongerminal center B-cell-like type. The proliferative activity was determined using immunostaining with the Ki-67 antibody. Of 18 cases, 16 (89%) were found to belong to the nongerminal center B-cell-like type. Two cases (11%) were classified as germinal center B-cell-like type; one had a CD10-positive, Bcl-6-positive and MUM1-negative profile, and the other was CD10 negative, Bcl-6 positive and MUM1 negative. The former occurred in a 38-year-old patient who was human immunodeficiency virus positive. All the cases expressed high proliferative activity (> or =50% Ki-67 labeling). We conclude that most (89%) primary testicular lymphomas of the diffuse large B-cell type belong to the nongerminal center B-cell-like subgroup and have high proliferative activity.
authors.affiliation: Department of Pathology, Wayne State University, Detroit, MI, USA. alabbadi@dmc.org
authors.forename: Mousa A
authors.initials: MA
authors.lastname: Al-Abbadi
authors.forename: Eyas M
authors.initials: EM
authors.lastname: Hattab
authors.forename: Musleh S
authors.initials: MS
authors.lastname: Tarawneh
authors.forename: Samir S
authors.initials: SS
authors.lastname: Amr
authors.forename: Attilio
authors.initials: A
authors.lastname: Orazi
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Ulbright
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D050835:Interferon Regulatory Factors / Q000032:analysis; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000401:mortality / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000737:chemistry / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D015260:Neprilysin / Q000032:analysis; D011379:Prognosis; D051560:Proto-Oncogene Proteins c-bcl-6; D015996:Survival Rate; D013736:Testicular Neoplasms / Q000737:chemistry / Q000401:mortality / Q000473:pathology*; D013737:Testis / Q000737:chemistry / Q000473:pathology; D000099218:Interferon Regulatory Factor-4
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D050835:Interferon Regulatory Factors; D015260:Neprilysin; D051560:Proto-Oncogene Proteins c-bcl-6; D000099218:Interferon Regulatory Factor-4; C084103:BCL6 protein, human