title: Simultaneous determination of carbohydrates, carboxylic acids, alcohols, and metals in foods by high-performance liquid chromatography inductively coupled plasma atomic emission spectrometry.
abstract: The applicability of the HPLC-ICP-AES coupling for the simultaneous determination of carbohydrates, carboxylic acids, alcohols, and metals in a single chromatographic run has been demonstrated in the present work. Five saccharides, glucose, fructose, sucrose, sorbitol, and lactose; five carboxylic acids, citric, tartaric, malic, lactic, and acetic; and three alcohols, glycerol, ethanol, and methanol, have been determined. A H+ cation exchange column has been used to separate these compounds. The chromatograms have been obtained by monitoring the carbon emission signal at 193.09 nm. The results obtained by HPLC-ICP-AES have been compared against those found with conventional detection systems (i.e., refractive index, UV, and photodyode array detectors). The HPLC-ICP-AES method has shown the following features: (i) organic compounds and metals can be simultaneously determined; (ii) the detection method is universal; (iii) for nonvolatile organic compounds, a complete calibration line can be obtained from a single injection; and (iv) it provides absolute limits of detection similar to or lower than those found with conventional detection systems (i.e., on the order of several tens of nanograms of organic compound). The methodology has been validated through the analysis of food samples such as juices, isotonic beverages, wines, and a certified nonfat milk powder sample.
authors.affiliation: Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Alicante, 03080 Alicante, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Paredes
authors.forename: Salvador E
authors.initials: SE
authors.lastname: Maestre
authors.forename: Soledad
authors.initials: S
authors.lastname: Prats
authors.forename: José L
authors.initials: JL
authors.lastname: Todolí
mesh_terms: D000438:Alcohols / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis; D008670:Metals / Q000032:analysis*; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D000438:Alcohols; D002241:Carbohydrates; D002264:Carboxylic Acids; D008670:Metals