title: Quantitative and functional differences between peripheral blood myeloid dendritic cells from patients with pleural and parenchymal lung tuberculosis.
abstract: Dendritic cells (DCs) play a pivotal role in generating protective host immunity to Mycobacterium tuberculosis. Few studies have addressed DC function in the context of active tuberculosis (TB), largely due to technical constraints in obtaining adequate numbers of DC from sick patients. We quantitated peripheral blood myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) in the whole blood of patients with active TB and show that blood from patients with pleural TB was characterized by high circulating levels of mDCs. We also developed and optimized a novel whole-blood assay to study mDC production of the Th1-promoting cytokine interleukin 12 (IL-12) and upregulation of the maturation marker CCR7 after incubation with mycobacteria. We found that pleural TB was associated with increased IL-12 production and CCR7 expression compared to lung parenchymal disease. Our findings suggest important differences in innate immunity between patients with different forms of active TB, and this may contribute to the differences in natural history observed between the two groups.
authors.affiliation: Laboratory of Mycobcterial Immunity and Pathogenesis, Public Health Research Institute, International Center for Public Health, Newark, New Jersey 07103-3535, USA. mmendels@curie.uct.ac.za
authors.forename: Marc
authors.initials: M
authors.lastname: Mendelson
authors.forename: Willem A
authors.initials: WA
authors.lastname: Hanekom
authors.forename: Siyabulela
authors.initials: S
authors.lastname: Ntutela
authors.forename: Monica
authors.initials: M
authors.lastname: Vogt
authors.forename: Lafras
authors.initials: L
authors.lastname: Steyn
authors.forename: Gary
authors.initials: G
authors.lastname: Maartens
authors.forename: Gilla
authors.initials: G
authors.lastname: Kaplan
mesh_terms: D000328:Adult; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D018664:Interleukin-12 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000166:cytology / Q000276:immunology*; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000378:metabolism; D014396:Tuberculosis, Pleural / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
chemical_list: C515370:CCR7 protein, human; D054400:Receptors, CCR7; D019707:Receptors, Chemokine; D018664:Interleukin-12