title: Palmitylation of cone opsins.
abstract: Palmitylation is a widespread modification in G-protein-coupled receptors and often a dynamic process. In rhodopsins, palmitylation is static on C322/C323. Red/green (M/LWS) cone opsins have no cysteines at corresponding positions and no palmitylation. Blue (SWS2) cone opsins have a single corresponding cysteine and mass spectrometric analysis showed partial palmitylation of salamander SWS2 cone opsin. Ultraviolet (SWS1) cone opsins have one corresponding cysteine, but only unpalmitylated opsin was observed for mouse and salamander. The results show that the static palmitylation found on rhodopsin is not found on cone opsins and suggest the possibility of an unidentified role for opsin palmitylation in cones.
authors.affiliation: Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA. ablonczy@musc.edu
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Ablonczy
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kono
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Knapp
authors.forename: Rosalie K
authors.initials: RK
authors.lastname: Crouch
mesh_terms: D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002417:Cattle; D002522:Chlorocebus aethiops; D002852:Chromatography, Ion Exchange; D008116:Lizards; D051379:Mice; D008969:Molecular Sequence Data; D019308:Palmitic Acid / Q000378:metabolism*; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism*; D012243:Rhodopsin / Q000235:genetics / Q000378:metabolism; D017299:Rod Opsins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013057:Spectrum Analysis; D014562:Caudata
chemical_list: D011994:Recombinant Proteins; D017299:Rod Opsins; D019308:Palmitic Acid; D012243:Rhodopsin