title: Analysis of the interaction between IFN-gamma and IFN-gammaR in the effector phase of experimental murine allergic conjunctivitis.
abstract: IFN-gamma acts to promote the severity of experimental allergic conjunctivitis (EC) during the effector phase. To identify the cell source and cell target of this cytokine in the effector phase of EC, we established mice lacking both IFN-gamma and the IFN-gamma receptor (Dbl-KO). Reciprocal adoptive transfer experiments involving wild-type, Dbl-KO, IFN-gamma-lacking and IFN-gammaR-lacking mice were performed. EC was then induced by RW challenge in eye drops. Analysis of the resulting eosinophil infiltration in the six donor/recipient combinations revealed that IFN-gamma produced by both donor and recipient cells plays an important role in the EC effector phase, and that the targets for this cytokine are also both donor and recipient cells. That EC was attenuated when any of the IFN-gamma-IFN-gammaR interactions were disturbed confirms that IFN-gamma promotes the severity of EC during the effector phase. These observations indicate that Dbl-KO mice will be useful for investigating the role(s) IFN-gamma play in inflammatory diseases.
authors.affiliation: Department of Ophthalmology and Visual Science, Kochi Medical School, Japan. fukusima@med.koichi-u.ac.jp
authors.forename: Atsuki
authors.initials: A
authors.lastname: Fukushima
authors.forename: Tamaki
authors.initials: T
authors.lastname: Sumi
authors.forename: Ken
authors.initials: K
authors.lastname: Fukuda
authors.forename: Naoki
authors.initials: N
authors.lastname: Kumagai
authors.forename: Teruo
authors.initials: T
authors.lastname: Nishida
authors.forename: Emi
authors.initials: E
authors.lastname: Tsuru
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Ueno
mesh_terms: D019264:Adoptive Transfer; D031164:Ambrosia / Q000276:immunology; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000637:transplantation; D003233:Conjunctivitis, Allergic / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D017471:Receptors, Interferon / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D000096743:Interferon gamma Receptor
chemical_list: D007371:Interferon-gamma; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor