[Effect of overdose fluoride on the expression of enamelin in rat mandibular incisor].
OBJECTIVE
To observe the effect of overdose fluoride on the expression of enamelin in rat mandibular incisor.


METHODS
Twenty Wistar rats were divided randomly into two groups. Animals were maintained in standard environment with free access to food and distilled water (control group) or water added with 100 mg/L F-(experimental group). The rats were killed in the eighth week. HE staining was used to observe the morphology of ameloblasts. Immunohistochemical staining was adopted to study the expressions of enamelin in rat incisor.


RESULTS
The ameloblasts of the treated rat were arranged in multi-layer. The ameloblasts in group II were thinner than those in group I. The structure of enamel matrix was in disorder. The expressions of enamelin in ameloblasts and odontoblasts were obviously inhibited in group II (P < 0.01).


CONCLUSIONS
The overdose fluoride inhibits the secretion of enamelin and leads to the abnormal development of enamel matrix.
Liu
Tian-Lin
TL
Dept. of Oral Medicine, Hospital of Stomatology, Tongji University, Shanghai 200072, China.
Wang
Qiang
Q
Xu
Zhi-Ling
ZL
Guo
Li-Ying
LY
Jing
Feng-Qiu
FQ
Wu
Gang
G
D000565:Ameloblasts; D000818:Animals; D003743:Dental Enamel; D003746:Dental Enamel Proteins; D005459:Fluorides; D007180:Incisor; D008334:Mandible; D009804:Odontoblasts; D010710:Phosphates; D051381:Rats; D017208:Rats, Wistar
D003746:Dental Enamel Proteins; D010710:Phosphates; C038456:tuftelin; C012980:fluorophosphate; D005459:Fluorides