title: Role of proteins in oil-in-water emulsions on the stability of lipid hydroperoxides.
abstract: The purpose of this research was to better understand the mechanisms by which proteins affect the rates of lipid oxidation in order to develop protein-stabilized emulsion delivery systems with maximal oxidative stability. This study evaluated the affect of pH and emulsifier concentration on the stability of cumene hydroperoxide in hexadecane-in-water emulsions stabilized by beta-lactoglobulin (beta-Lg). Emulsions prepared with 0.2 wt % beta-Lg (at pH 7.0) showed a 26.9% decrease in hydroperoxide concentrations 5 min after 0.25 mM ferrous ion was added to the emulsion. EDTA, but not continuous phase beta-Lg, could inhibit iron-promoted lipid hydroperoxide decomposition. Lipid hydroperoxides were more stable to iron-promoted degradation at pH values below the pI of beta-Lg, where the emulsion droplet would be cationic and thus able to repel iron away from the lipid hydroperoxides. Heating the beta-Lg-stabilized emulsions to produce a cohesive protein layer on the emulsion droplet surface did not alter the ability of iron to decompose lipid hydroperoxides. These results suggest that proteins at the interface of emulsion droplets primarily stabilize lipid hydroperoxides by electrostatically inhibiting iron-hydroperoxide interactions.
authors.affiliation: Department of Food Science, Chenoweth Laboratory, University of Massachusetts, Amherst, Massachusetts 01003, USA.
authors.forename: Sarah S
authors.initials: SS
authors.lastname: Kellerby
authors.forename: D Julian
authors.initials: DJ
authors.lastname: McClements
authors.forename: Eric A
authors.initials: EA
authors.lastname: Decker
mesh_terms: D000473:Alkanes; D001555:Benzene Derivatives / Q000737:chemistry; D004355:Drug Stability; D004655:Emulsions / Q000737:chemistry*; D005296:Ferrous Compounds / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007782:Lactoglobulins / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000737:chemistry*; D011506:Proteins / Q000494:pharmacology*; D055672:Static Electricity; D014867:Water
chemical_list: D000473:Alkanes; D001555:Benzene Derivatives; D004655:Emulsions; D005296:Ferrous Compounds; D007782:Lactoglobulins; D008054:Lipid Peroxides; D011506:Proteins; D014867:Water; C007932:n-hexadecane; C007164:cumene hydroperoxide