Utilising the left-helical conformation of L-DNA for analysing different marker types on a single universal microarray platform.
L-DNA is the perfect mirror-image form of the naturally occurring d-conformation of DNA. Therefore, L-DNA duplexes have the same physical characteristics in terms of solubility, duplex stability and selectivity as D-DNA but form a left-helical double-helix. Because of its chiral difference, L-DNA does not bind to its naturally occurring D-DNA counterpart, however. We analysed some of the properties that are typical for L-DNA. For all the differences, L-DNA is chemically compatible with the D-form of DNA, so that chimeric molecules can be synthesized. We take advantage of the characteristics of L-DNA toward the establishment of a universal microarray that permits the analysis of different kinds of molecular diagnostic information in a single experiment on a single platform, in various combinations. Typical results for the measurement of transcript level variations, genotypic differences and DNA-protein interactions are presented. However, on the basis of the characteristic features of L-DNA, also other applications of this molecule type are discussed.
Hauser
Nicole C
NC
Genomics-Proteomics-Systemsbiology, Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik Nobelstrasse 12, 70569 Stuttgart, Germany. nicole.hauser@igb.fraunhofer.de
Martinez
Rafael
R
Jacob
Anette
A
Rupp
Steffen
S
Hoheisel
Jörg D
JD
Matysiak
Stefan
S
D000818:Animals; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D005819:Genetic Markers; D025202:Molecular Diagnostic Techniques; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D020641:Polymorphism, Single Nucleotide; D013237:Stereoisomerism
D004268:DNA-Binding Proteins; D005819:Genetic Markers; D015345:Oligonucleotide Probes; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D003851:Deoxyribonucleases