title: Differential regulation of L-arginine transporters (cationic amino acid transporter-1 and -2) by peroxynitrite in rat mesangial cells.
abstract: BACKGROUND
  It has become evident that increased nitric oxide (NO) generation may be associated with production of reactive oxygen species, such as peroxynitrite (ONOO-). Peroxynitrite has been postulated to be responsible for several of the cytotoxic effects previously ascribed to NO. Since cellular arginine uptake has been shown to modulate nitric oxide synthase activity, we were intrigued to study the effect of ONOO- on arginine traffic in renal mesangial cells.
  
  
  METHODS
  Arginine uptake, CAT-1 and CAT-2 mRNA expression by northern blotting analysis, and CAT-1 protein content using western blotting were determined in mesangial cells pre-treated with peroxynitrite (0.1 and 0.5 mM) for 2 h.
  
  
  RESULTS
  Peroxynitrite induced a significant increase in arginine uptake and CAT-2 mRNA expression compared with untreated cells. In contrast, CAT-1 mRNA expression and protein abundance were diminished.
  
  
  CONCLUSIONS
  In rat mesangial cells, peroxynitrite augments arginine uptake via augmentation of CAT-2 while decreasing CAT-1 expression.
authors.affiliation: Department of Nephrology, Tel Aviv Sourasky Medical Center, 6 Weizman Street, Tel Aviv, Israel. nmdri@netvision.net.il
authors.forename: Idit Feenberg
authors.initials: IF
authors.lastname: Schwartz
authors.forename: Tamara
authors.initials: T
authors.lastname: Chernichovsky
authors.forename: David
authors.initials: D
authors.lastname: Hagin
authors.forename: Meirav
authors.initials: M
authors.lastname: Ingbir
authors.forename: Ran
authors.initials: R
authors.lastname: Reshef
authors.forename: Gil
authors.initials: G
authors.lastname: Chernin
authors.forename: Yoram
authors.initials: Y
authors.lastname: Levo
authors.forename: Doron
authors.initials: D
authors.lastname: Schwartz
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism*; D027201:Cationic Amino Acid Transporter 1 / Q000235:genetics / Q000502:physiology*; D027202:Cationic Amino Acid Transporter 2 / Q000235:genetics / Q000502:physiology*; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism*; D030421:Peroxynitrous Acid / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D027201:Cationic Amino Acid Transporter 1; D027202:Cationic Amino Acid Transporter 2; D012333:RNA, Messenger; D030421:Peroxynitrous Acid; D001120:Arginine