title: Isolation and characterization of heparan sulfate from various murine tissues.
abstract: Heparan sulfate (HS), is a proteoglycan (PG) found both in the extracellular matrix and on cell surface. It may represent one of the most biologically important glycoconjugates, playing an essential role in a variety of different events at molecular level. The publication of the mouse genome, and the intensive investigations aimed at understanding the proteome it encodes, has motivated us to initiate studies in mouse glycomics focused on HS. The current study is aimed at determining the quantitative and qualitative organ distribution of HS in mice. HS from brain, eyes, heart, lung, liver, kidney, spleen, intestine and skin was purified from 6-8 week old male and female mice. The recovered yield of HS from these organs is compared with the recovered whole body yield of HS. Structural characterization of the resulting HS relied on disaccharide analysis and (1)H-NMR spectroscopy. Different organs revealed a characteristic HS structure. These data begin to provide a structural understanding of the role of HS in cell-cell interactions, cell signaling and sub-cellular protein trafficking as well as a fundamental understanding of certain aspects of protein-carbohydrate interactions.
authors.affiliation: Department of Biochemistry- Faculty of Veterinary Medicine, Cairo University, Cairo.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Warda
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Toida
authors.forename: Fuming
authors.initials: F
authors.lastname: Zhang
authors.forename: Peilong
authors.initials: P
authors.lastname: Sun
authors.forename: Eva
authors.initials: E
authors.lastname: Munoz
authors.forename: Jin
authors.initials: J
authors.lastname: Xie
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D000818:Animals; D004187:Disaccharides / Q000032:analysis / Q000737:chemistry; D005260:Female; D019763:Heparin Lyase; D006497:Heparan Sulfate / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D014018:Tissue Distribution
chemical_list: D004187:Disaccharides; D019763:Heparin Lyase; D006497:Heparan Sulfate