Coaggregation interactions between oral and endodontic Enterococcus faecalis and bacterial species isolated from persistent apical periodontitis.
Interactions between Enterococcus faecalis and other species found in root canal infections might be important for the development and persistence of periapical disease. The aim of this study was to investigate the coaggregation interactions between E. faecalis clinical isolates and species previously shown to survive and induce apical periodontitis in monkeys: Peptostreptococcus anaerobius, Prevotella oralis, Fusobacterium nucleatum, and Streptococcus anginosus. Intergeneric coaggregation assays were conducted in duplicate with observations scored immediately at 0 h, 1 h and 24 h after mixing of combinations of strains. All E. faecalis strains (n = 53) coaggregated with F. nucleatum; E. faecalis did not coaggregate with P. anaerobius or S. anginosus. One strain, E. faecalis E1, coaggregated with P. oralis, with aggregates visible at 1 h. Coaggregation interactions between E. faecalis and F. nucleatum observed in this study suggest a potential role for this combination in endodontic infections.
Johnson
Erika M
EM
Department of Cariology, Restorative Sciences and Endodontics, The University of Michigan, School of Dentistry, Ann Arbor, Michigan 48109-1078, USA.
Flannagan
Susan E
SE
Sedgley
Christine M
CM
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D001431:Bacteriological Techniques; D003786:Dental Pulp Cavity / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000145:classification / Q000502:physiology*; D016967:Fusobacterium nucleatum / Q000502:physiology; D000882:Haplorhini; D009055:Mouth / Q000382:microbiology*; D010462:Peptostreptococcus / Q000502:physiology; D010485:Periapical Periodontitis / Q000382:microbiology*; D018720:Prevotella / Q000502:physiology; D034366:Streptococcus anginosus / Q000502:physiology; D013997:Time Factors