Kyotorphin suppresses proliferation and Ca2+ signaling in brown preadipocytes.
A positive correlation was revealed between stimulation of protein and DNA synthesis in preadipocytes by norepinephrine or neokyotorphin and intracellular Ca2+ concentration in these cells. Kyotorphin abolished the stimulatory effect of norepinephrine on proliferation of cultured cells and cold-induced [3H]-thymidine incorporation into DNA of mouse brown adipose tissue in vivo. These changes correlated with peptide-induced suppression of slow calcium signaling in preadipocytes.
Bronnikov
G E
GE
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino. gennady_bronnikov@rambler.ru
Kolaeva
S G
SG
Dolgacheva
L P
LP
Kramarova
L I
LI
D002001:Adipose Tissue, Brown / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003080:Cold Temperature; D004247:DNA / Q000096:biosynthesis; D004723:Endorphins / Q000008:administration & dosage / Q000494:pharmacology*; D066298:In Vitro Techniques; D051379:Mice; D009638:Norepinephrine / Q000008:administration & dosage / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D022722:Thermogenesis / Q000502:physiology
D004723:Endorphins; C019448:kyotorphin; C036450:neo-kyotorphin; D004247:DNA; D009638:Norepinephrine