title: Denaturation of proteins in methanol/water mixtures.
abstract: The solvophobic theory developed earlier by Sinanoglu introducing the use of molecular surface areas and microthermodynamic surface and interfacial tensions at molecular dimensions is applied to the interpretation of calorimetric data on denaturation of lysozyme in a wide range of methanol/water mixtures. The experimental values of standard unitary free energies of denaturation correlate well with our predictions. The molecular surface area change of the protein upon denaturation is evaluated using the solvophobic theory. The maximum in the stability of the native form of the protein is predicted to occur at 8% (v/v) methanol. This is found to be in agreement with the experimental results.
authors.affiliation: Departments of Chemistry and of Molecular Biochemistry/Biophysics, Yale University, New Haven, CT 06520, USA.
authors.forename: A
authors.initials: A
authors.lastname: Fernández
authors.forename: O
authors.initials: O
authors.lastname: Sinanoglu