title: Adhesion of Yersinia enterocolitica to non-cultured epithelial cells from pig and rabbit ilea.
abstract: A simple and reliable method was developed to isolate intact epithelial cells from pig and rabbit ilea and these were used to investigate the adhesion of Yersinia enterocolitica. Hydrophobic interaction was eliminated by treating the bacterial culture with 0.8 M tetramethyl urea (TMU). Virulent strains of Y. enterocolitica had significantly greater attachment than avirulent strains but both attached in a linear dose-dependant fashion. Epithelial cells prepared from pig ilea were attached to more readily than those prepared from rabbit ilea.
authors.affiliation: Institute of Veterinary, Animal and Biomedical Sciences, College of Science, Massey University, Palmerston North, New Zealand. Hassan.Hussein@agresearch.co.nz
authors.forename: Hassan M
authors.initials: HM
authors.lastname: Hussein
authors.forename: Stan G
authors.initials: SG
authors.lastname: Fenwick
authors.forename: John S
authors.initials: JS
authors.lastname: Lumsden
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005767:Gastrointestinal Diseases / Q000382:microbiology* / Q000473:pathology; D007082:Ileum / Q000382:microbiology / Q000473:pathology; D011817:Rabbits; D013552:Swine; D014774:Virulence; D015009:Yersinia Infections / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000502:physiology*