Analysis of detergent-resistant membranes associated with apical and basolateral GPI-anchored proteins in polarized epithelial cells.
Detergent-resistant membranes (DRMs) represent specialized membrane domains resistant to detergent extraction, which may serve to segregate proteins in a specific environment in order to improve their function. Segregation of glycosylphosphatidylinositol-anchored proteins (GPI-APs) in DRMs has been shown to be involved in their sorting to the apical membrane in polarized epithelial cells. Nonetheless, we have shown that both apical and basolateral GPI-APs associate with DRMs. In this report we investigated the lipid composition of DRMs associated with an apical and a basolateral GPI-AP. We found that apical and basolateral DRMs contain the same lipid species although in different ratios. This specific lipid ratio is maintained after mixing the cells before lysis indicating that DRMs maintain their identity after Triton extraction.
Tivodar
Simona
S
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università degli Studi di Napoli Federico II, via S. Pansini 5, 80131 Napoli, Italy.
Paladino
Simona
S
Pillich
Rudolf
R
Prinetti
Alessandro
A
Chigorno
Vanna
V
van Meer
Gerrit
G
Sonnino
Sandro
S
Zurzolo
Chiara
C
D000818:Animals; D002457:Cell Extracts; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D016764:Cell Polarity; D003902:Detergents / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects; D008565:Membrane Proteins / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats
D002457:Cell Extracts; D003902:Detergents; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins