title: Development of a real-time PCR assay for detection of gyrA mutations associated with reduced susceptibility to ciprofloxacin in Salmonella enterica serovar typhi and paratyphi A.
abstract: A real-time PCR assay with the cycling probe method was used to detect mutations at codons 83 and 87 in the DNA gyrase A subunit encoded by gyrA in Salmonella enterica serovar Typhi and Paratyphi A clinical isolates. The susceptibility estimated from the results of the gyrA mutation assay was consistent with that identified by the culture method using an E-test. This assay allows rapid screening of S. enterica serovar Typhi and Paratyphi A with reduced susceptibility to ciprofloxacin.
authors.affiliation: Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Masatomo
authors.initials: M
authors.lastname: Morita
authors.forename: Kenitiro
authors.initials: K
authors.lastname: Ito
authors.forename: Kenji
authors.initials: K
authors.lastname: Hirose
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Ken
authors.initials: K
authors.lastname: Shimuta
authors.forename: Jun
authors.initials: J
authors.lastname: Terajima
authors.forename: Makoto
authors.initials: M
authors.lastname: Ohnishi
authors.forename: Makoto
authors.initials: M
authors.lastname: Harada
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Matsuzaki
authors.forename: Haruo
authors.initials: H
authors.lastname: Watanabe
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Izumiya
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D006801:Humans; D009154:Mutation; D010284:Paratyphoid Fever / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012482:Salmonella paratyphi A / Q000187:drug effects* / Q000235:genetics; D012485:Salmonella typhi / Q000187:drug effects* / Q000235:genetics; D014435:Typhoid Fever / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D027081:DNA Gyrase