Immunoproteomic analysis of capsulate and non-capsulate strains of Lactococcus garvieae.
A comparative immunoproteomic study was carried out to investigate the immunogenicity of capsulate (KG9408) and non-capsulate (NSS9310) strains of Lactococcus garvieae. Immunoblot assays, following two-dimensional gel electrophoresis (2-DE) for L. garvieae strains, revealed a significant difference between anti-capsulate and anti-non-capsulate rabbit sera with respect to the number and antigenicity of antigenic spots. Anti-capsulate and anti-non-capsulate rabbit sera reacted with an average of 72 and 127 antigenic spots, respectively. The strong reaction of anti-non-capsulate sera with elongation factor (EF)-G and -Tu, and GMP synthase, of the L. garvieae strains identifies these as specific major antigens. This study clearly demonstrates the differences in 2-DE immunoblot profiles between the capsulate and non-capsulate strains of L. garvieae. These differences may be the reason for variations in immunogenicity between capsulate and non-capsulate strains. Glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, arginine deaminase and ornithine carbamoyltransferase were identified from the 2-DE immunoblot profiles of both strains. Therefore, these common antigens are potential markers for the development of vaccines against L. garvieae, irrespective of strain. Immunoproteomics, a powerful tool for studying antigens at the proteomic level, allowed a comparative investigation of the immunogenicity of capsulate and non-capsulate strains of L. garvieae for vaccine development.
Shin
G W
GW
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, South Korea.
Palaksha
K J
KJ
Kim
Y R
YR
Nho
S W
SW
Cho
J H
JH
Heo
N E
NE
Heo
G J
GJ
Park
S C
SC
Jung
T S
TS
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D016667:Bacterial Capsules / Q000737:chemistry / Q000145:classification / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D007106:Immune Sera; D015151:Immunoblotting / Q000662:veterinary; D017045:Lactococcus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D020543:Proteome / Q000235:genetics / Q000276:immunology; D040901:Proteomics; D011817:Rabbits
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera; D020543:Proteome