Biochemical investigations of bowel inflammation in gastroschisis.
Neonates with gastroschisis have perivisceritis resulting from contact between the bowel and amniotic fluid. Here, we characterized the mediators involved in this inflammatory process in humans and ewes, to find a reliable marker of this process. We have diagnosed 41 cases of gastroschisis since 1995. Amniotic fluid sampled for karyotyping between 15 and 32 wk of gestation was also used to assay cytokines and inflammatory proteins. The findings were compared with those in 93 age-matched controls. Amniotic fluid cells were analyzed by means of cytology. Histologic examination of the bowel was performed when neonatal appendectomy was performed. The findings were compared with those obtained in a ewe model of gastroschisis. In gastroschisis, amniotic total protein, IL-6, IL-8, and ferritin levels were significantly higher than in controls. Gastroschisis was associated with significantly higher cell counts (mainly mononuclear cells) in amniotic fluid. At birth, macrophages were abundant in the fibrous peel covering the bowel. Similar results were obtained in the ewe model. Gastroschisis may be associated with a subchronic inflammatory process of variable intensity. This inflammation is restricted to the bowel wall and involves inflammatory cells such as macrophages, which may secrete ferritin, neopterin, and calprotectin.
Guibourdenche
Jean
J
Department of Biology, Hôpital Robert Debré APHP, 75235 Paris, France.
Berrebi
Dominique
D
Vuillard
Edith
E
de Lagausie
Pascal
P
Aigrain
Yves
Y
Oury
Jean-François
JF
Luton
Dominique
D
D000653:Amniotic Fluid / Q000737:chemistry / Q000166:cytology; D000818:Animals; D005260:Female; D020139:Gastroschisis / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005865:Gestational Age; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000276:immunology / Q000473:pathology; D011247:Pregnancy; D012756:Sheep