title: Use of RNA fingerprinting to identify fungal genes specifically expressed during ectomycorrhizal interaction.
abstract: The ecosystem soil is characterized by interactions between microorganisms and plants including mycorrhiza--mutualistic interactions between fungi and plant roots. Species of the basidiomycete genus Tricholoma form ectomycorrhiza with tree roots which is characterized by morphological and metabolic changes of both partners, yet molecular mechanisms of the interaction are poorly understood. We performed differential display with arbitrarily primed RT-PCR using ectomycorrhiza between the basidiomycete Tricholoma vaccinum and its compatible host spruce (Picea abies) to isolate mycorrhiza-specific fungal gene fragments. 76 differentially expressed PCR fragments were verified and checked for plant or fungal origin and expression pattern. Of 20 fungal fragments with mycorrhiza-specific expression, sequence analyses were performed to identify homologs with known function of the encoded protein. Among the genes identified were orthologs to an aldehyde dehydrogenase, an alcohol dehydrogenase and a protein of the MATE transporter family, all with possible function in plant pathogen response. A phospholipase B, a beta-glucosidase and a binding protein of basic amino acids might play a role in nutrient exchange and growth in planta. A protein similar to inactive E2 compounds of ubiquitin-conjugating enzymes like CROC-1 and MMS2, a Ras protein and an APS kinase were placed in signal transduction and two retrotransposons of the Ty3-gypsy and the Ty1-copia family are expressed most likely due to stress.
authors.affiliation: University of Jena, Dept. Microbiology, Microbial Phytopathology, Neugasse 25, D-07743 Jena, Germany. Katrin.Krause@uni-jena.de
authors.forename: Katrin
authors.initials: K
authors.lastname: Krause
authors.forename: Erika
authors.initials: E
authors.lastname: Kothe
mesh_terms: D000363:Agaricales / Q000235:genetics*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D038821:Mycorrhizae / Q000235:genetics*; D028222:Picea / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D013559:Symbiosis