title: Up-regulation of NPY gene expression in hypothalamus of rats with experimental chronic renal failure.
abstract: Anorexia is possibly one of the most important causes of malnutrition in uremic patients. The cause of this abnormality is still unknown. Considering that: (a) NPY is one of the most important stimulants of food intake; (b) eating is a central nervous system regulated process and (c) NPY is expressed in hypothalamus, we hypothesized that the decrease of NPY gene expression in the hypothalamus could be an important factor contributing to anorexia associated with uremic state. In contrast to the prediction, the results presented in this paper indicate that the NPY gene expression in the hypothalamus of chronic renal failure (CRF) rats was significantly higher than in the hypothalamus of control (pair-fed) rats. Moreover, we found that serum NPY concentration in CRF rats was higher than in control (pair-fed) animals. The increase of plasma NPY concentration in CRF rats may be due to the greater synthesis of the neuropeptide in liver, since higher level of NPY mRNA was found in liver of CRF rats. The results obtained revealed that experimental chronic renal failure is associated with the increase of NPY gene expression in hypothalamus and liver of rats.
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine Medical University of Gdansk, 80-211 Gdansk, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Sucajtys-Szulc
authors.forename: Joanna
authors.initials: J
authors.lastname: Karbowska
authors.forename: Zdzislaw
authors.initials: Z
authors.lastname: Kochan
authors.forename: Wojciech
authors.initials: W
authors.lastname: Wolyniec
authors.forename: Michal
authors.initials: M
authors.lastname: Chmielewski
authors.forename: Boleslaw
authors.initials: B
authors.lastname: Rutkowski
authors.forename: Julian
authors.initials: J
authors.lastname: Swierczynski
mesh_terms: D000818:Animals; D000855:Anorexia / Q000503:physiopathology; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000737:chemistry / Q000378:metabolism*; D007676:Kidney Failure, Chronic / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D008099:Liver / Q000378:metabolism; D008297:Male; D009478:Neuropeptide Y / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation
chemical_list: D009478:Neuropeptide Y; D012333:RNA, Messenger