title: [Effects of prenatal stress on kinetic properties of high-voltage-activated Ca2+ channel in freshly isolated offspring rat hippocampal CA3 pyramidal neurons].
abstract: OBJECTIVE
  To investigate the effects of prenatal stress (PNS) on kinetic properties of high-voltage-activated (HVA) Ca(2+) channel in freshly isolated offspring rat hippocampal CA3 pyramidal neurons.
  
  
  METHODS
  The pregnant rats were exposed to restraint stress, and the hippocampal CA3 pyramidal neurons were freshly isolated from the offspring rats to record voltage-gate Ca(2+) channel currents in these neurons using whole-cell configuration of patch-clamp technique.
  
  
  RESULTS
  PNS significantly increased HVA calcium current density and integral current but did not alter the steady-state activation and steady-state inactivation properties of HVA Ca(2+) channel in the offspring rat CA3 neurons. The maximal HVA calcium current density was -40.89-/+0.31 pA/pF in the control group (n=10) and -49.44-/+0.37 pA/pF in PNS group (n=8, P<0.01). The maximal integral current of the HVA Ca(2+) channel was 106.81-/+4.20 nA*ms in the control group (n=10) and 133.49-/+2.59 nA*ms in the PNS group (n=8, P<0.01).
  
  
  CONCLUSIONS
  Maternal exposure to stress during the critical phase of pregnancy may result in long-lasting effects on the ion channels of the hippocampal neurons in the offspring rats.
authors.affiliation: Department of Physiology and Pathophysiology, Key Laboratory of Environment and Gene Related to Diseases of Ministry of Education, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. caiqabc@163.com
authors.forename: Qing
authors.initials: Q
authors.lastname: Cai
authors.forename: Zhong-liang
authors.initials: ZL
authors.lastname: Zhu
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Fan
authors.forename: Ning
authors.initials: N
authors.lastname: Jia
authors.forename: Zhuan-li
authors.initials: ZL
authors.lastname: Bai
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Song
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015220:Calcium Channels / Q000502:physiology*; D005260:Female; D006624:Hippocampus / Q000166:cytology; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D018408:Patch-Clamp Techniques; D011247:Pregnancy; D011248:Pregnancy Complications / Q000523:psychology; D017966:Pyramidal Cells / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical / Q000523:psychology; D013315:Stress, Psychological / Q000503:physiopathology*
chemical_list: D015220:Calcium Channels