Protein composition of the vaccinia virus mature virion.
The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugations and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass spectrometry to detect tryptic peptides separated by high-resolution liquid chromatography. Eighty vaccinia virus-encoded proteins representing 37% of the 218 genes annotated in the complete genome sequence were detected in at least three analyses. Ten proteins accounted for approximately 80% of the virion mass. Thirteen identified proteins were not previously reported as components of virions. On the other hand, 8 previously described virion proteins were not detected here, presumably due to technical reasons including small size and hydrophobicity. In addition to vaccinia virus-encoded proteins, 24 host proteins omitting isoforms were detected. The most abundant of these were cytoskeletal proteins, heat shock proteins and proteins involved in translation.
Resch
Wolfgang
W
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4 Center Drive, MSC 0445, Bethesda, MD 20892-0445, USA.
Hixson
Kim K
KK
Moore
Ronald J
RJ
Lipton
Mary S
MS
Moss
Bernard
B
D002500:Centrifugation, Isopycnic; D002853:Chromatography, Liquid; D017550:Spectroscopy, Fourier Transform Infrared; D053719:Tandem Mass Spectrometry; D014616:Vaccinia virus / Q000737:chemistry* / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis*; D014771:Virion / Q000737:chemistry* / Q000302:isolation & purification
D014764:Viral Proteins