Differential transcription profiles in Trypanosoma cruzi associated with clinical forms of Chagas disease: Maxicircle NADH dehydrogenase subunit 7 gene truncation in asymptomatic patient isolates.
The majority of individuals in the chronic phase of Chagas disease are asymptomatic (indeterminate form). Every year 2-3% of these individuals develop severe clinical manifestations (cardiac and digestive forms). In this study a Trypanosoma cruzi DNA microarray was used to compare the transcript profiles of six human isolates: three from asymptomatic and three from cardiac patients. Seven signals were expressed differentially between the two classes of isolates, including tryparedoxin, surface protease GP63, cyclophilin, some hypothetical proteins and the pre-edited maxicircle gene NADH dehydrogenase subunit 7 (ND7). The approximately 30-fold greater signal in cardiac strains for ND7 was the most pronounced of the group, and differential levels of pre-edited ND7 transcript confirmed the microarray analysis. The ND7 gene from asymptomatic isolates showed a deletion of 455bp from nt 222 to nt 677 relative to ND7 of the CL Brener reference strain. The ND7 gene structure correlated with disease manifestation for 20 isolates from clinically characterised, chronic phase patients. The ND7 lesion produces a truncated product that could impair the function of mitochondrial complex I. Possible links between the integrity of the electron transport chain and symptom presentation are discussed. We propose that ND7 and other genes of the pathway constitute valuable targets for PCR assays in the differential diagnosis of the infective T. cruzi strain. While this hypothesis requires validation by the examination of additional recent parasite isolates from patients with defined pathologies, the identification of specific molecular markers represents a promising advance in the association between parasite genetics and disease pathology.
Baptista
Cassio S
CS
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Avenida Professor Lineu Prestes, 748 CEP 05508-000, São Paulo, SP, Brazil.
Vêncio
Ricardo Z N
RZ
Abdala
Sarah
S
Carranza
Julio César
JC
Westenberger
Scott J
SJ
Silva
Marcelo N
MN
Pereira
Carlos A de B
CA
Galvão
Lúcia M C
LM
Gontijo
Eliane D
ED
Chiari
Egler
E
Sturm
Nancy R
NR
Zingales
Bianca
B
D000293:Adolescent; D000328:Adult; D000818:Animals; D002598:Chagas Cardiomyopathy / Q000469:parasitology*; D014355:Chagas Disease / Q000469:parasitology*; D018105:DNA, Kinetoplast / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D015800:Protozoan Proteins / Q000235:genetics*; D014349:Trypanosoma cruzi / Q000235:genetics*
D018105:DNA, Kinetoplast; D008565:Membrane Proteins; C107339:ND7 protein, Protozoa; D015800:Protozoan Proteins; D009245:NADH Dehydrogenase