title: Two new disposable bioreactors for plant cell culture: The wave and undertow bioreactor and the slug bubble bioreactor.
abstract: The present article describes two novel flexible plastic-based disposable bioreactors. The first one, the WU bioreactor, is based on the principle of a wave and undertow mechanism that provides agitation while offering convenient mixing and aeration to the plant cell culture contained within the bioreactor. The second one is a high aspect ratio bubble column bioreactor, where agitation and aeration are achieved through the intermittent generation of large diameter bubbles, "Taylor-like" or "slug bubbles" (SB bioreactor). It allows an easy volume increase from a few liters to larger volumes up to several hundred liters with the use of multiple units. The cultivation of tobacco and soya cells producing isoflavones is described up to 70 and 100 L working volume for the SB bioreactor and WU bioreactor, respectively. The bioreactors being disposable and pre-sterilized before use, cleaning, sterilization, and maintenance operations are strongly reduced or eliminated. Both bioreactors represent efficient and low cost cell culture systems, applicable to various cell cultures at small and medium scale, complementary to traditional stainless-steel bioreactors.
authors.affiliation: Centre de Recherche & Développement Nestlé-Tours, 101, Avenue Gustave Eiffel, BP 49716, 37097 Tours Cedex 2, France. benedicte.terrier@rdto.nestle.com
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Terrier
authors.forename: Didier
authors.initials: D
authors.lastname: Courtois
authors.forename: Nicolas
authors.initials: N
authors.lastname: Hénault
authors.forename: Arnaud
authors.initials: A
authors.lastname: Cuvier
authors.forename: Maryse
authors.initials: M
authors.lastname: Bastin
authors.forename: Aziz
authors.initials: A
authors.lastname: Aknin
authors.forename: Julien
authors.initials: J
authors.lastname: Dubreuil
authors.forename: Vincent
authors.initials: V
authors.lastname: Pétiard
mesh_terms: D019149:Bioreactors / Q000191:economics; D018929:Cell Culture Techniques; D004867:Equipment Design / Q000191:economics / Q000379:methods*; D059828:Plant Cells