Construction and characterization of a novel maltose inducible expression vector in Bacillus subtilis.
A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two genes, bioA and vgb. This thus provided a potential expression system for cloned genes in B. subtilis.
Ming-Ming
Yang
Y
Institute of Biochemical Engineering, College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, P.R. China.
Wei-Wei
Zhang
Z
Xi-Feng
Zhang
Z
Pei-Lin
Cen
C
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D004790:Enzyme Induction; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005822:Genetic Vectors / Q000096:biosynthesis / Q000235:genetics*; D006420:Hemeproteins / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D009876:Operon / Q000235:genetics*; D011401:Promoter Regions, Genetic; D000637:Transaminases / Q000378:metabolism; D014169:Transformation, Bacterial; D001616:beta-Galactosidase / Q000378:metabolism
D001426:Bacterial Proteins; D006420:Hemeproteins; C100771:Vgb protein, Bacteria; D008320:Maltose; D000637:Transaminases; C102100:BioA protein, bacteria; D001616:beta-Galactosidase