Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichia coli.
The docosahexaenoic acid (DHA) biosynthesis gene cluster (pDHA3) from the DHA-producing Moritella marina strain MP-1 includes the genes pfaA, pfaB, pfaC, and pfaD, which are similar to the genes of polyketide biosynthesis. When this cluster was co-expressed in Escherichia coli with M. marina MP-1 pfaE, which encodes phosphopantetheinyl transferase, DHA was biosynthesized. The maximum production of DHA (5% of total fatty acids) was observed at 15 degrees C. This is the first report of the recombinant production of DHA in a polyketide biosynthesis mode.
Orikasa
Yoshitake
Y
Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, 060-0810, Japan.
Nishida
Takanori
T
Yamada
Akiko
A
Yu
Reiko
R
Watanabe
Kazuo
K
Hase
Akira
A
Morita
Naoki
N
Okuyama
Hidetoshi
H
D001709:Biotechnology / Q000379:methods*; D002849:Chromatography, Gas; D002853:Chromatography, Liquid / Q000379:methods; D004281:Docosahexaenoic Acids / Q000737:chemistry* / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology*; D005227:Fatty Acids / Q000096:biosynthesis / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D013058:Mass Spectrometry; D008956:Models, Chemical; D008957:Models, Genetic; D034221:Moritella / Q000378:metabolism; D005810:Multigene Family; D010957:Plasmids / Q000378:metabolism; D013696:Temperature
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid