title: The DNA-binding domain of CaNdt80p is required to activate CDR1 involved in drug resistance in Candida albicans.
abstract: CaNdt80p, the Candida albicans homologue of the Saccharomyces cerevisiae transcription factor ScNdt80p, has been identified as a positive regulator of CDR1, which encodes an efflux pump involved in drug resistance in C. albicans. To investigate the involvement of the putative DNA-binding domain of CaNdt80p in drug resistance, chimeras of CaNdt80p and ScNdt80p were constructed. Interestingly, the DNA-binding domain of ScNdt80p could functionally complement that of CaNdt80p to activate CDR1p-lacZ in S. cerevisiae. Consistently, CaNdt80p containing a mutation in the DNA-binding domain failed to activate CDR1p-lacZ in S. cerevisiae. Furthermore, a copy of CaNDT80 with the same mutation also failed to complement the drug-sensitive phenotype caused by a null mutation in C. albicans. Thus, the DNA-binding domain of CaNdt80p is critical for its function in drug resistance in C. albicans.
authors.affiliation: Division of Clinical Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, Republic of China.|Graduate Institute of Life Sciences, National Defence Medical Center, Taipei, Taiwan, Republic of China.
authors.forename: Jang-Shiun
authors.initials: JS
authors.lastname: Wang
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China.
authors.forename: Yun-Liang
authors.initials: YL
authors.lastname: Yang
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China.
authors.forename: Chin-Jung
authors.initials: CJ
authors.lastname: Wu
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China.
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Ouyang
authors.affiliation: Division of Clinical Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, Republic of China.
authors.forename: Kuo-Yun
authors.initials: KY
authors.lastname: Tseng
authors.affiliation: Division of Clinical Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, Republic of China.
authors.forename: Chia-Geun
authors.initials: CG
authors.lastname: Chen
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China.
authors.forename: Hsin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Division of Clinical Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, Republic of China.
authors.forename: Hsiu-Jung
authors.initials: HJ
authors.lastname: Lo
mesh_terms: D000595:Amino Acid Sequence; D002176:Candida albicans / Q000737:chemistry / Q000187:drug effects*; D004271:DNA, Fungal / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D005816:Genetic Complementation Test; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C094693:CDR1 protein, Candida albicans; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; C495215:Ndt80 protein, Candida albicans; D014157:Transcription Factors