Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression.
Agrobacterium-mediated transient expression may be regarded as a promising method for inexpensive large-scale production of recombinant proteins. We optimized the protocol of transient expression in Nicotiana benthamiana and compared six Australian species of Nicotiana as hosts for transient expression. The transient expression of GFP under 35S CaMV promoter was observed in all species tested, although the GFP content in leaves of N. benthamiana, N. exigua, and N. excelsior was significantly higher (3.8, 3.7, and 2.0% TSP, respectively). Usage of viral-based expression system resulted in considerable increase of GFP accumulation in N. excelsior and N. benthamiana (63.5 and 16.2% TSP, respectively). We displayed that N. excelsior has the best characteristics in regard to biomass yield as well as GFP accumulation level for both types of the expression cassettes tested.
Sheludko
Y V
YV
Institute of Cell Biology and Genetic Engineering, Zabolotnogo Str. 148, Kyiv 03143, Ukraine. ysheludko@fromru.com
Sindarovska
Y R
YR
Gerasymenko
I M
IM
Bannikova
M A
MA
Kuchuk
N V
NV
D036022:Capsid Proteins / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017930:Genes, Reporter; D005822:Genetic Vectors; D018515:Plant Leaves / Q000235:genetics*; D010942:Plant Viruses / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012231:Rhizobium / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*; D019076:Transgenes
D036022:Capsid Proteins