Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot.
In order to study the multiple functions of fibrinogen and fibrin, we are investigating which proteins bind to the fibrin matrix of a plasma clot by using a proteomic approach. Extracts from washed plasma clots were analysed by 2-D gel electrophoresis. A relatively abundant spot was identified as hepatocyte-derived fibrinogen-related protein-1 (HFREP-1) by MALDI-TOF analysis, molecular mass (34 kDa), iso-electric point (pI 5.5) as well as by Western blot analysis. HFREP-1 in plasma almost completely bound to the fibrin matrix during clot formation. Several purified fibrinogen preparations proved to be contaminated with HFREP-1. It is concluded that HFREP-1 (also named hepassocin), a protein with liver cell growth regulatory properties, occurs in plasma and strongly associates with fibrin and possibly fibrinogen.
Rijken
D C
DC
Department of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands.
Dirkx
S P G
SP
Luider
T M
TM
Leebeek
F W G
FW
D000818:Animals; D002417:Cattle; D005337:Fibrin / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D010949:Plasma / Q000378:metabolism*; D011485:Protein Binding
C485894:FGL1 protein, human; D009363:Neoplasm Proteins; D005337:Fibrin; D005340:Fibrinogen