title: Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichia coli.
abstract: The docosahexaenoic acid (DHA) biosynthesis gene cluster (pDHA3) from the DHA-producing Moritella marina strain MP-1 includes the genes pfaA, pfaB, pfaC, and pfaD, which are similar to the genes of polyketide biosynthesis. When this cluster was co-expressed in Escherichia coli with M. marina MP-1 pfaE, which encodes phosphopantetheinyl transferase, DHA was biosynthesized. The maximum production of DHA (5% of total fatty acids) was observed at 15 degrees C. This is the first report of the recombinant production of DHA in a polyketide biosynthesis mode.
authors.affiliation: Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, 060-0810, Japan.
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Orikasa
authors.forename: Takanori
authors.initials: T
authors.lastname: Nishida
authors.forename: Akiko
authors.initials: A
authors.lastname: Yamada
authors.forename: Reiko
authors.initials: R
authors.lastname: Yu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Watanabe
authors.forename: Akira
authors.initials: A
authors.lastname: Hase
authors.forename: Naoki
authors.initials: N
authors.lastname: Morita
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Okuyama
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002849:Chromatography, Gas; D002853:Chromatography, Liquid / Q000379:methods; D004281:Docosahexaenoic Acids / Q000737:chemistry* / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology*; D005227:Fatty Acids / Q000096:biosynthesis / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D013058:Mass Spectrometry; D008956:Models, Chemical; D008957:Models, Genetic; D034221:Moritella / Q000378:metabolism; D005810:Multigene Family; D010957:Plasmids / Q000378:metabolism; D013696:Temperature
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid