title: Possible involvement of ATP-purinoceptor signalling in the intercellular synchronization of intracellular Ca2+ oscillation in cultured cardiac myocytes.
abstract: Isolated and cultured neonatal cardiac myocytes contract spontaneously and cyclically. The contraction rhythms of two isolated cardiac myocytes, each of which beats at different frequencies at first, become synchronized after the establishment of mutual contacts, suggesting that mutual entrainment occurs due to electrical and/or mechanical interactions between two myocytes. The intracellular concentration of free Ca(2+) also changes rhythmically in association with the rhythmic contraction of myocytes (Ca(2+) oscillation), and such a Ca(2+) oscillation was also synchronized among cultured cardiac myocytes. In this study, we investigated whether intercellular communication other than via gap junctions was involved in the intercellular synchronization of intracellular Ca(2+) oscillation in spontaneously beating cultured cardiac myocytes. Treatment with either blockers of gap junction channels or an un-coupler of E-C coupling did not affect the intercellular synchronization of Ca(2+) oscillation. In contrast, treatment with a blocker of P2 purinoceptors resulted in the asynchronization of Ca(2+) oscillatory rhythms among cardiac myocytes. The present study suggested that the extracellular ATP-purinoceptor system was responsible for the intercellular synchronization of Ca(2+) oscillation among cardiac myocytes.
authors.affiliation: Laboratory of Cellular Cybernetics, Graduate School of Information Science and Technology, Hokkaido University, Sapporo 060-0814, Japan.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Nakayama
authors.forename: Koichi
authors.initials: K
authors.lastname: Kawahara
authors.forename: Takeru
authors.initials: T
authors.lastname: Hachiro
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Yamauchi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Yoneyama
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002450:Cell Communication; D017629:Gap Junctions; D007091:Image Processing, Computer-Assisted; D008954:Models, Biological; D032383:Myocytes, Cardiac / Q000166:cytology*; D009991:Oscillometry; D051381:Rats; D017208:Rats, Wistar; D011983:Receptors, Purinergic / Q000378:metabolism*; D015398:Signal Transduction; D049490:Systems Biology
chemical_list: D011983:Receptors, Purinergic; D000255:Adenosine Triphosphate; D002118:Calcium