Effect of Epstein-Barr virus infection on global gene expression in nasopharyngeal carcinoma.
It was proposed that Epstein-Barr virus (EBV) is closely associated with nasopharyngeal carcinoma (NPC); however, the molecular mechanisms involved in the effect of EBV on NPC host genes have not yet been well defined. For this study, two sets of microarray experiments, NPC (EBV-free) vs normal epithelial cells and EBV(+) vs EBV(-) NPC arrays, were analyzed and the datasets were cross-compared to identify any correlation between gene clusters involved in EBV targeting and the NPC host gene expression profiles. Statistical analysis revealed that EBV seems to have a preference for targeting more genes from the differentially expressed group in NPC cells than those from the ubiquitously expressed group. Furthermore, this trend is also reflected in log ratios where the EBV target genes of the differentially expressed group origin showed greater log ratios than genes with an origin from the ubiquitously expressed NPC group. Taken together, the genome-wide comparative scanning of EBV and NPC transcriptomes has successfully demonstrated that EBV infection has an intensifying effect on the signals involved in NPC gene expression both in breadth (the majority of the genes) and in depth (greater log ratios).
Lee
Yuan-Chii Gladys
YC
Graduate Institute of Biomedical Informatics, Taipei Medical University, Taipei, Taiwan, Republic of China.
Hwang
Yu-Chyi
YC
Chen
Kuang-Chi
KC
Lin
Yong-Shiang
YS
Huang
Dah-Yeou
DY
Huang
Tao-Wei
TW
Kao
Cheng-Yan
CY
Wu
Han-Chung
HC
Lin
Chin-Tarng
CT
Huang
Chi-Ying F
CY
D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D002460:Cell Line; D002478:Cells, Cultured; D020031:Epstein-Barr Virus Infections / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000821:virology*