Sensitization of surfactants on the chemiluminescence reaction of fluorescein isothiocyanate labeled proteins.
The effect of surfactants on fluorescein isothiocyanate (FITC)-bovine serum albumin (BSA)-hypochlorite (ClO(-)), FITC-human serum albumin (HSA)-ClO(-), FITC-ovoconalbumin (OVA)-ClO(-), FITC-hemoglobin (Hb)-ClO(-) systems were investigated with chemiluminescence method by the reversed phase flow injection. It was found that the chemiluminescence (CL) intensity of each system was increased greatly in the presence of cationic surfactants. Cethyltrimethylammonium bromide (CTAB) is the optimal surfactant of these systems. The optimal conditions of the CL reaction and the optimal concentration of CTAB were examined and the function of cationic surfactant CTAB on the CL reaction was also discussed.
Huang
Chunbao
C
Department of Chemistry, Huanggang Normal University, Huangzhou 438000, China.
Zhang
Kai
K
Ci
Yunxiang
Y
D000818:Animals; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D017022:Flow Injection Analysis; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D005456:Fluorescent Dyes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D008163:Luminescent Measurements; D010047:Ovalbumin; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D013501:Surface-Active Agents
D002593:Cetrimonium Compounds; D005456:Fluorescent Dyes; D011506:Proteins; D012709:Serum Albumin; D013501:Surface-Active Agents; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D016650:Fluorescein-5-isothiocyanate; D000077286:Cetrimonium