A time- and cost-efficient system for high-level protein production in mammalian cells.
Most proteins for structural biology studies are produced by high-level expression in Escherichia coli. However, prokaryotic based expression systems fail to generate correctly folded functional forms of many proteins and hence a variety of eukaryotic based expression systems have been developed. Of these, yeast and baculovirus-infected insect cells currently represent the expression systems of choice for structural biologists. Here, protocols for a simple, fast and affordable method for transient protein expression in mammalian cells are reported. The results demonstrate that it combines several features necessary for the production of suitable samples for structural biology, in particular protein crystallography, namely high protein yield, straightforward purification, selenomethionine incorporation and control of N-linked glycosylation. The system is suitable for use in conventional laboratories or can be implemented in a medium- or high-throughput pipeline.
Aricescu
A Radu
AR
Cancer Research UK Receptor Structure Research Group, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, England.
Lu
Weixian
W
Jones
E Yvonne
EY
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D003362:Cost-Benefit Analysis; D018360:Crystallography, X-Ray; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D005822:Genetic Vectors; D006031:Glycosylation; D006801:Humans; D007202:Indicators and Reagents; D008322:Mammals; D040901:Proteomics / Q000191:economics* / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000191:economics* / Q000302:isolation & purification; D012645:Selenomethionine / Q000378:metabolism; D014162:Transfection / Q000379:methods
D007202:Indicators and Reagents; D011994:Recombinant Proteins; D004247:DNA; D012645:Selenomethionine