title: Aberrant FHIT protein expression in classical Hodgkin's lymphoma: a potential marker.
abstract: OBJECTIVE
  The fragile histidine triad (FHIT) gene is frequently inactivated in human cancers; however, the FHIT gene remains unexplored in Hodgkin's lymphoma. The aim of this study was to investigate the role of FHIT expression in classical Hodgkin's lymphoma.
  
  
  METHODS
  Classical Hodgkin's lymphomas were analysed for FHIT gene expression by two-step non-biotin immunohistochemical method and Western blotting.
  
  
  RESULTS
  Thirty of the 33 (91%) cases of Hodgkin's lymphoma tested were positive for FHIT protein by immuohistochemistry. The expression of FHIT was mainly located in cytoplasm of Reed-Sternberg (HRS) cells. The protein expression was also documented by Western blotting. The non-Hodgkin's lymphomas were negative for FHIT protein.
  
  
  CONCLUSIONS
  The results indicate that abnormal FHIT expression is noted frequently in classical Hodgkin's lymphoma and the expression can give insight into the pathogenesis of the disease. The protein may serve as a marker to localise HRS cells in classical Hodgkin's lymphoma.
authors.affiliation: Department of Pathology, General Hospital of Chinese People's Liberation Army, Beijing, People's Republic of China. zhaopo@plagh.com.cn
authors.forename: Po
authors.initials: P
authors.lastname: Zhao
authors.forename: Alfred King Yin
authors.initials: AK
authors.lastname: Lam
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Lu
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
authors.forename: Ling-Hong
authors.initials: LH
authors.lastname: Chen
authors.forename: Xiao-Lu
authors.initials: XL
authors.lastname: Pu
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D005260:Female; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D006689:Hodgkin Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D016539:Reed-Sternberg Cells / Q000378:metabolism / Q000473:pathology; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase