Genetic characterization of the phenylacetyl-coenzyme A oxygenase from the aerobic phenylacetic acid degradation pathway of Escherichia coli.
We show here that the paaABCDE genes of the paa cluster responsible for phenylacetate degradation in Escherichia coli W encode a five-component oxygenase that hydroxylates phenylacetyl-coenzyme A (CoA), the first intermediate of the pathway. The primary structure of the subunits of bacterial phenylacetyl-CoA oxygenases revealed that these enzymes constitute the prototype of a new and distinct group of the large bacterial diiron multicomponent oxygenase family.
Fernández
Cristina
C
Department of Molecular Microbiology, Centro de Invetigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040 Madrid, Spain.
Ferrández
Abel
A
Miñambres
Baltasar
B
Díaz
Eduardo
E
García
José L
JL
D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000332:Aerobiosis; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D019143:Evolution, Molecular; D010648:Phenylacetates / Q000378:metabolism; D010802:Phylogeny; D010957:Plasmids
D010648:Phenylacetates; D000105:Acetyl Coenzyme A; C037816:phenylacetyl-coenzyme A; C025136:phenylacetic acid