Serodiagnosis of Neospora caninum infection in cattle using a recombinant tNcSRS2 protein-based ELISA.
The truncated NcSRS2 gene (tNcSRS2) by removal of the N-terminal hydrophobic sequence was cloned into the pGEX-6p-1 plasmid and subsequently expressed as a glutathione-S-transferase (GST) fusion protein. The purified recombinant tNcSRS2 protein was specific to Neospora caninum and was used in an ELISA for the diagnosis of neosporosis. There was a good agreement between tNctSRS2-based ELISA and three commercially available diagnostic kits (IDEXX ELISA, HIPRA ELISA and VMRD IFAT). Three hundred dairy cattle serum samples from 9 regions were tested by our ELISA. The herd prevalence was 100% and the overall prevalence was 20.3%. There was a statistically significant difference in seroprevalence between regions (P<0.01) and an association between abortion history and N. caninum seropositivity. Our study showed that neosporosis in dairy cattle is widespread in China.
Liu
Jing
J
Parasitology Laboratory, Department of Veterinary Preventive Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Yu
Jinshu
J
Wang
Ming
M
Liu
Qun
Q
Zhang
Wei
W
Deng
Chong
C
Ding
Jun
J
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000453:epidemiology*; D002681:China / Q000453:epidemiology; D003048:Coccidiosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D018688:Neospora / Q000276:immunology*; D011993:Recombinant Fusion Proteins; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D011993:Recombinant Fusion Proteins