Automatic internal calibration in liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry of protein digests.
Accurately measured peptide masses can be used for large-scale protein identification from bacterial whole-cell digests as an alternative to tandem mass spectrometry (MS/MS) provided mass measurement errors of a few parts-per-million (ppm) are obtained. Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) routinely achieves such mass accuracy either with internal calibration or by regulating the charge in the analyzer cell. We have developed a novel and automated method for internal calibration of liquid chromatography (LC)/FTICR data from whole-cell digests using peptides in the sample identified by concurrent MS/MS together with ambient polydimethylcyclosiloxanes as internal calibrants in the mass spectra. The method reduced mass measurement error from 4.3 +/- 3.7 ppm to 0.3 +/- 2.3 ppm in an E. coli LC/FTICR dataset of 1000 MS and MS/MS spectra and is applicable to all analyses of complex protein digests by FTICRMS.
Palmblad
Magnus
M
The BioCentre, The University of Reading, Reading, UK. n.m.palmblad@reading.ac.uk
Bindschedler
Laurence V
LV
Gibson
Trevor M
TM
Cramer
Rainer
R
D000465:Algorithms; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods / Q000592:standards*; D017357:Cyclotrons / Q000295:instrumentation / Q000592:standards; D029968:Escherichia coli Proteins / Q000032:analysis*; D012371:Robotics / Q000295:instrumentation* / Q000379:methods / Q000592:standards*; D012984:Software; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods / Q000592:standards*; D006113:United Kingdom
D029968:Escherichia coli Proteins