title: Detection of human-derived fecal pollution in environmental waters by use of a PCR-based human polyomavirus assay.
abstract: Regulatory agencies mandate the use of fecal coliforms, Escherichia coli or Enterococcus spp., as microbial indicators of recreational water quality. These indicators of fecal pollution do not identify the specific sources of pollution and at times underestimate health risks associated with recreational water use. This study proposes the use of human polyomaviruses (HPyVs), which are widespread among human populations, as indicators of human fecal pollution. A method was developed to concentrate and extract HPyV DNA from environmental water samples and then to amplify it by nested PCR. HPyVs were detected in as little as 1 microl of sewage and were not amplified from dairy cow or pig wastes. Environmental water samples were screened for the presence of HPyVs and two additional markers of human fecal pollution: the Enterococcus faecium esp gene and the 16S rRNA gene of human-associated Bacteroides. The presence of human-specific indicators of fecal pollution was compared to fecal coliform and Enterococcus concentrations. HPyVs were detected in 19 of 20 (95%) samples containing the E. faecium esp gene and Bacteroides human markers. Weak or no correlation was observed between the presence/absence of human-associated indicators and counts of indicator bacteria. The sensitivity, specificity, and correlation with other human-associated markers suggest that the HPyV assay could be a useful predictor of human fecal pollution in environmental waters and an important component of the microbial-source-tracking "toolbox."
authors.affiliation: Department of Microbiology and Cell Science, University of Florida, Gainesville 33611, USA.
authors.forename: Shannon M
authors.initials: SM
authors.lastname: McQuaig
authors.forename: Troy M
authors.initials: TM
authors.lastname: Scott
authors.forename: Valerie J
authors.initials: VJ
authors.lastname: Harwood
authors.forename: Samuel R
authors.initials: SR
authors.lastname: Farrah
authors.forename: Jerzy O
authors.initials: JO
authors.lastname: Lukasik
mesh_terms: D000818:Animals; D001439:Bacteroides / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000235:genetics / Q000302:isolation & purification; D016983:Enterococcus / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology / Q000821:virology*; D005618:Fresh Water / Q000382:microbiology / Q000821:virology; D006801:Humans; D007202:Indicators and Reagents; D016015:Logistic Models; D016133:Polymerase Chain Reaction / Q000379:methods*; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012722:Sewage / Q000382:microbiology / Q000821:virology; D013552:Swine; D014876:Water Pollution / Q000032:analysis*
chemical_list: D004279:DNA, Viral; D007202:Indicators and Reagents; D012722:Sewage