title: Genome-wide microRNA profiling in human fetal nervous tissues by oligonucleotide microarray.
abstract: OBJECTIVE
  Our objective was to develop an oligonucleotide DNA microarray (OMA) for genome-wide microRNA profiling and use this method to find miRNAs, which control organic development especially for nervous system.
  
  
  METHODS
  Eighteen organic samples included cerebrum and spinal cord samples from two aborted human fetuses. One was 12 gestational weeks old (G12w) and the other was 24 gestational weeks old (G24w). Global miRNA expression patterns of different organs were investigated using OMA and Northern blot.
  
  
  CONCLUSIONS
  The OMA revealed that 72-83% of miRNAs were expressed in human fetal organs. A series of microRNAs were found specifically and higher-expressed in the human fetal nervous system and confirmed consistently by Northern blot, which may play a critical role in nervous system development.
authors.affiliation: Department of Genetics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Zhao
authors.forename: You-Jia
authors.initials: YJ
authors.lastname: Hua
authors.forename: Da-Guang
authors.initials: DG
authors.lastname: Sun
authors.forename: Xian-Xin
authors.initials: XX
authors.lastname: Meng
authors.forename: Hua-Sheng
authors.initials: HS
authors.lastname: Xiao
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
mesh_terms: D000367:Age Factors; D005333:Fetus; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009417:Nerve Tissue / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013997:Time Factors
chemical_list: D035683:MicroRNAs