title: HIV type 1 zidovudine (ZDV) resistance in blood and uterine cervical secretions of pregnant women.
abstract: To determine if HIV-1 resistance testing of the blood predicts mutants in the genital secretions of ZDV-treated pregnant women, ZDV resistance mutations were assessed by an oligonucleotide ligation assay. ZDV resistance was detected in viral sequences from blood (16/48; 33%) and cervical secretions (6/24; 25%) collected during 57 pregnancies. The genotype of 11/69 (16%) resistance codons was discordant between blood and cervical virus of pregnant women near term. However, in only 1 (1.8%) of 57 pregnancies evaluated was resistant virus limited to the cervical secretions. ZDV-resistant virus is rarely limited to the female genital tract.
authors.affiliation: University of Washington, Seattle, Washington 98109., Children's Hospital and Medical Center of Seattle, Seattle, Washington 98105, USA. lfrenkel@u.washington.edu
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Frenkel
authors.forename: Jennifer
authors.initials: J
authors.lastname: McKernan
authors.forename: Phil V
authors.initials: PV
authors.lastname: Dinh
authors.forename: Deborah
authors.initials: D
authors.lastname: Goldman
authors.forename: Jane
authors.initials: J
authors.lastname: Hitti
authors.forename: D Heather
authors.initials: DH
authors.lastname: Watts
authors.forename: Ellen R
authors.initials: ER
authors.lastname: Cooper
authors.forename: Joan
authors.initials: J
authors.lastname: Dragavon
authors.forename: Robert W
authors.initials: RW
authors.lastname: Coombs
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D002582:Cervix Mucus / Q000821:virology; D024882:Drug Resistance, Viral / Q000235:genetics* / Q000502:physiology; D005260:Female; D005836:Genitalia, Female / Q000821:virology; D015658:HIV Infections / Q000188:drug therapy / Q000517:prevention & control*; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control; D010949:Plasma / Q000821:virology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D015215:Zidovudine / Q000494:pharmacology*
chemical_list: D019380:Anti-HIV Agents; D015415:Biomarkers; D015215:Zidovudine; D012194:RNA-Directed DNA Polymerase