Transcription inhibition using oligonucleotide-modified gold nanoparticles.
The capture of T7 RNA polymerase using double-stranded promoter DNA on the surface of gold nanoparticles has been demonstrated. The competitive binding and inhibition of T7 RNA polymerase due to specific interactions on the nanoparticle surface represents a transcription factor decoy approach in a model system. The efficiency of inhibition was determined for various nanoparticle sizes, surface coverage, and linker length for double-stranded promoter DNA on gold nanoparticles. The experiments provide a basis for determining the accessibility of binding sites on nanoparticle surfaces for applications involving cell targeting or the use of nanoparticles as binding agents in solution.
Agbasi-Porter
Chiamaka
C
Department of Chemistry, North Carolina State University, Raleigh, 27695, USA.
Ryman-Rasmussen
Jessica
J
Franzen
Stefan
S
Feldheim
Daniel
D
D002460:Cell Line; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D009841:Oligonucleotides; D014764:Viral Proteins; D006046:Gold; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases