Characterization of cytosolic glutathione S-transferases in California Halibut (Paralichthys californicus).
Two cytosolic glutathione S-transferase (GST) classes were isolated and characterized from California halibut (Paralichthys californicus) liver. Nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to elucidate peptide sequences and the proteins were identified as theta and alpha by searching against the NCBI non-redundant database (nrDB). Catalytic activity of the cytosolic GSTs towards 1-chloro-2,4-dinitrobenzene (CDNB) was determined to be 0.23+/-0.003 U/mg cytosolic protein.
Donham
Rachel T
RT
Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California, 1 Shields Avenue, Davis, CA 95616-8588, USA.
Luna
Andrea D
AD
Chang
Sandra
S
Morin
Dexter
D
Jewell
William T
WT
Tjeerdema
Ronald S
RS
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004137:Dinitrochlorobenzene; D005432:Flounder / Q000378:metabolism*; D005982:Glutathione Transferase / Q000302:isolation & purification / Q000378:metabolism*; D022781:Hepatocytes / Q000201:enzymology
D004137:Dinitrochlorobenzene; D005982:Glutathione Transferase