Molecular identification of Trichuris vulpis and Trichuris suis isolated from different hosts.
Trichuris suis was isolated from the cecum of two different hosts (Sus scrofa domestica -- swine and Sus scrofa scrofa -- wild boar) and Trichuris vulpis from dogs in Sevilla, Spain. Genomic DNA was isolated and internal transcribed spacers (ITS)1-5.8S-ITS2 segment from the ribosomal DNA (rDNA) was amplified and sequenced using polymerase chain reaction techniques. The sequence of T. suis from both hosts was 1,396 bp in length while that of T. vulpis was 1,044 bp. ITS1 of both populations isolated of T. suis was 661 nucleotides in length, while the ITS2 was 534 nucleotides in length. Furthermore, the ITS1 of T. vulpis was 410 nucleotides in length, while the ITS2 was 433 nucleotides in length. One hundred fifty-four nucleotides were observed along the 5.8S gene of T. suis and T. vulpis. Intraindividual and intraspecific variations were detected in the rDNA of both species. The presence of microsatellites was observed in all the individuals assayed. Sequence analysis of the ITSs and the 5.8S gene has demonstrated no sequence differences between T. suis isolated from both hosts (S. scrofa domestica -- swine and S. scrofa scrofa -- wild boar). Nevertheless, clear differences were detected between the ITS1 and ITS2 of T. suis and T. vulpis. Furthermore, a comparative molecular analysis between both species and the previously published ITS1-5.8S-ITS2 sequence data of Trichuris ovis, Trichuris leporis, Trichuris muris, Trichuris arvicolae, and Trichuris skrjabini was carried out. A common homology zone was detected in the ITS1 sequence of all species of trichurids.
Cutillas
Cristina
C
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Profesor García González s/n., Sevilla, Spain. cutillas@us.es
de Rojas
Manuel
M
Ariza
Concepción
C
Ubeda
José Manuel
JM
Guevara
Diego
D
D000818:Animals; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D034421:Sus scrofa / Q000469:parasitology; D013552:Swine / Q000469:parasitology; D013553:Swine Diseases / Q000469:parasitology; D014257:Trichuriasis / Q000469:parasitology / Q000662:veterinary; D014258:Trichuris / Q000145:classification* / Q000235:genetics*
D021903:DNA, Ribosomal Spacer