An interdisciplinary approach to investigate the impact of cobalt in a human keratinocyte cell line.
Since in nuclear power plants, risks of skin contact contamination by radiocobalt are significant, we focused on the impact of cobalt on a human cutaneous cell line, i.e. HaCaT keratinocytes. The present paper reports an interdisciplinary approach aimed at clarifying the biochemical mechanisms of metabolism and toxicity of cobalt in HaCaT cells. Firstly, a brief overview of the used instrumental techniques is reported. The following parts present description and discussion of results concerning: (i) toxicological studies concerning cobalt impact towards HaCaT cells (ii) structural and speciation fundamental studies of cobalt-bioligand systems, through X-ray absorption spectroscopy (XAS), ab initio and thermodynamic modelling (iii) preliminary results regarding intracellular cobalt speciation in HaCaT cells using size exclusion chromatography/inductively coupled plasma-atomic emission spectroscopy (SEC/ICP-AES) and direct in situ analysis by ion beam micropobe analytical techniques.
Bresson
C
C
DEN/DANS/DPC/SECR/LSRM, CEA Saclay, Batiment 391, 91191 Gif-sur-Yvette, France.
Lamouroux
C
C
Sandre
C
C
Tabarant
M
M
Gault
N
N
Poncy
J L
JL
Lefaix
J L
JL
Den Auwer
C
C
Spezia
R
R
Gaigeot
M-P
MP
Ansoborlo
E
E
Mounicou
S
S
Fraysse
A
A
Deves
G
G
Bacquart
T
T
Seznec
H
H
Pouthier
T
T
Moretto
P
P
Ortega
R
R
Lobinski
R
R
Moulin
C
C
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003035:Cobalt / Q000493:pharmacokinetics / Q000633:toxicity*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
D009153:Mutagens; D003035:Cobalt