title: Identification of active transposon dTok, a member of the hAT family, in rice.
abstract: Recent completion of the sequencing of the rice genome has revealed that it contains >40% repetitive sequences, most of which are related to inactive transposable elements. During the molecular analysis of the floral organ number 1/multiple pistil 2 (fon1/mp2) mutant, we identified an active transposable element dTok0 that was inserted at the kinase domain of FON1, a homolog of CLAVATA1. Insertion of the element into FON1 generated an 8 bp duplication of its target sites, which is one of the major characteristics of the hAT family of transposons. The dTok0 element was actively transposed out of the FON1 gene, leaving 5-8 bp footprints. Reinsertion into a new location was observed at a low frequency. Analysis of the genome sequence showed that the rice cultivar 'Nipponbare' contains 25 copies of dTok elements; similar numbers were present in all the Oryza species examined. Because dTok0 does not encode a transposase, enzyme activity should be provided in trans. We identified a putative autonomous transposon, Tok1 that contains an intact open reading frame of the Ac-like transposase.
authors.affiliation: National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea.
authors.forename: Sunok
authors.initials: S
authors.lastname: Moon
authors.forename: Ki-Hong
authors.initials: KH
authors.lastname: Jung
authors.forename: Do-Eun
authors.initials: DE
authors.lastname: Lee
authors.forename: Wen-Zhu
authors.initials: WZ
authors.lastname: Jiang
authors.forename: Hee Jong
authors.initials: HJ
authors.lastname: Koh
authors.forename: Mun-Hue
authors.initials: MH
authors.lastname: Heu
authors.forename: Dong Sun
authors.initials: DS
authors.lastname: Lee
authors.forename: Hak Soo
authors.initials: HS
authors.lastname: Suh
authors.forename: Gynheung
authors.initials: G
authors.lastname: An
mesh_terms: D000483:Alleles; D001483:Base Sequence; D032461:Chromosomes, Plant / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D016254:Mutagenesis, Insertional / Q000235:genetics; D009154:Mutation / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements