Candida albicans binds to saliva proteins selectively adsorbed to silicone.
Explanted voice prostheses obtained from 5 patients at the time of prosthesis replacement were consistently colonized by yeast, in particular Candida albicans. A simple, reproducible, in vitro model of C. albicans adherence to saliva-coated voice prosthesis silicone was developed. Whole saliva promoted adherence of C. albicans to silicone in a dose-dependent manner. Saliva rinses from voice prosthesis patients also promoted binding of C. albicans to silicone in vitro (mean adherence 14.9% +/- 2.8% of input C. albicans cells). This was significantly higher than C. albicans adherence to silicone in the absence of saliva (P < .001) or adherence promoted by saliva rinses from healthy volunteers (P < .005). Polyacrylamide gel electrophoresis analysis and a blot overlay adherence assay revealed that certain salivary proteins were selectively adsorbed to silicone and that C. albicans yeast cells adhered specifically to the adsorbed salivary proteins.
Holmes
Ann R
AR
Department of Oral Sciences, School of Dentistry, University of Otago, Dunedin, New Zealand.
van der Wielen
Pauline
P
Cannon
Richard D
RD
Ruske
Dean
D
Dawes
Patrick
P
D000327:Adsorption; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018441:Biofilms; D002176:Candida albicans / Q000302:isolation & purification / Q000502:physiology*; D016022:Case-Control Studies; D002448:Cell Adhesion; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007831:Larynx, Artificial / Q000382:microbiology*; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism; D055232:Proline-Rich Protein Domains; D011475:Prosthesis Failure; D011485:Protein Binding; D012463:Saliva / Q000502:physiology*; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D012826:Silicone Elastomers
D010455:Peptides; D012471:Salivary Proteins and Peptides; D012826:Silicone Elastomers