Combined use of polycationic peptide and biodegradable macromolecular polymer as a novel gene delivery system: a preliminary study.
Plasmid(p) DNA was condensed by polycationic peptide polylysine (PLL) to be a core and then encapsulated in biodegradable monomethoxy (polyethyleneglycol)-poly(lactide-co-glycolide)-monomethoxy (poly-ethylene glycol) (PELGE) to form core-shell nanoparticles as a novel gene delivery system PPD (PELGE-PLL-DNA). Nanoparticles formed from PPD had several complementary properties such as improved biocompatibility, decreased cytotoxicity, enhanced pDNA integrity, and the characteristic of lysosomal escape as PLGA nanoparticles. The results demonstrated the potential of this PPD as an efficient gene delivery system.
Nie
Yu
Y
Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, PR China.
Zhang
Zhi-Rong
ZR
Duan
You-Rong
YR
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D004355:Drug Stability; D004563:Electrochemistry; D018014:Gene Transfer Techniques; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid; D008247:Lysosomes / Q000737:chemistry; D053758:Nanoparticles; D010316:Particle Size; D014677:Pharmaceutical Vehicles; D010957:Plasmids; D011092:Polyethylene Glycols; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011107:Polylysine / Q000737:chemistry*; D011108:Polymers; D014465:Ultrasonics
D014677:Pharmaceutical Vehicles; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011107:Polylysine; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; D004247:DNA