title: Effect of the antibiotic azithromycin on thermotropic behavior of DOPC or DPPC bilayers.
abstract: Azithromycin is a macrolide antibiotic known to bind to lipids and to affect endocytosis probably by interacting with lipid membranes [Tyteca, D., Schanck, A., Dufrene, Y.F., Deleu, M., Courtoy, P.J., Tulkens, P.M., Mingeot-Leclercq, M.P., 2003. The macrolide antibiotic azithromycin interacts with lipids and affects membrane organization and fluidity: studies on Langmuir-Blodgett monolayers, liposomes and J774 macrophages. J. Membr. Biol. 192, 203-215]. In this work, we investigate the effect of azithromycin on lipid model membranes made of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) or 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Thermal transitions of both lipids in contact with azithromycin are studied by (31)P NMR and DSC on multilamellar vesicles. Concerning the DPPC, azithromycin induces a suppression of the pretransition whereas a phase separation between the DOPC and the antibiotic is observed. For both lipids, the enthalpy associated with the phase transition is strongly decreased with azithromycin. Such effects may be due to an increase of the available space between hydrophobic chains after insertion of azithromycin in lipids. The findings provide a molecular insight of the phase merging of DPPC gel in DOPC fluid matrix induced by azithromycin [Berquand, A., Mingeot-Leclercq, M.P., Dufrene, Y.F., 2004. Real-time imaging of drug-membrane interactions by atomic force microscopy. Biochim. Biophys. Acta 1664, 198-205] and could help to a better understanding of azithromycin-cell interaction.
authors.affiliation: Unité de Pharmacologie Cellulaire et Moléculaire, Université Catholique de Louvain, 73.70 Avenue E. Mounier 73, B-1200 Brussels, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: Fa
authors.forename: S
authors.initials: S
authors.lastname: Ronkart
authors.forename: A
authors.initials: A
authors.lastname: Schanck
authors.forename: M
authors.initials: M
authors.lastname: Deleu
authors.forename: A
authors.initials: A
authors.lastname: Gaigneaux
authors.forename: E
authors.initials: E
authors.lastname: Goormaghtigh
authors.forename: M-P
authors.initials: MP
authors.lastname: Mingeot-Leclercq
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D017963:Azithromycin / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D044367:Phase Transition; D010713:Phosphatidylcholines / Q000737:chemistry*; D010761:Phosphorus Radioisotopes / Q000737:chemistry; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010761:Phosphorus Radioisotopes; D010767:Phosphorylcholine; D017963:Azithromycin; C017251:1,2-oleoylphosphatidylcholine