Cardenolide glycosides from Pergularia tomentosa and their proapoptotic activity in Kaposi's sarcoma cells.
Continuing our investigations on plants belonging to the Asclepiadaceae family, three new cardenolide glycosides, 3'-O-beta-D-glucopyranosylcalactin (1), 12-dehydroxyghalakinoside (2), and 6'-dehydroxyghalakinoside (3), along with the known ghalakinoside (4) and calactin (5), were isolated from the roots of Pergularia tomentosa. The structures of these compounds were elucidated by extensive spectroscopic methods including 1D- and 2D-NMR experiments as well as ESIMS analysis. The isolated cardenolides caused apoptotic cell death of Kaposi's sarcoma cells.
Hamed
Arafa I
AI
Faculty of Science, South Valley University, Aswan 81528, Egypt.
Plaza
Alberto
A
Balestrieri
Maria Luisa
ML
Mahalel
Usama A
UA
Springuel
Irina V
IV
Oleszek
Wieslaw
W
Pizza
Cosimo
C
Piacente
Sonia
S
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D029062:Apocynaceae / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects; D002298:Cardenolides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004534:Egypt; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012514:Sarcoma, Kaposi; D021241:Spectrometry, Mass, Electrospray Ionization; D014407:Tumor Cells, Cultured
C514397:12-dehydroxyghalakinoside; C514396:3'-O-beta-D-glucopyranosylcalactin; C514398:6'-dehydroxyghalakinoside; D000972:Antineoplastic Agents, Phytogenic; D002298:Cardenolides; D006027:Glycosides