title: Antidepressant principles of the roots of Polygala tenuifolia.
abstract: [(125)I]RTI-55-membrane binding assay-guided fractionation and separation of a water-soluble extract of the roots of Polygala tenuifolia gave five new oligosaccharide derivatives, polygalatenosides A-E (1-5). The structures of these new oligosaccharides were established on the basis of spectroscopic evidence. Polygalatenosides A and B (1 and 2) showed significant inhibitory activity, with IC(50) values of 30.0 and 6.04 microM, respectively, in this membrane binding assay and acted as norepinephrine reuptake inhibitors through blocking norepinephrine transport.
authors.affiliation: Medical and Pharmaceutical Industry Technology and Development Center, Taipei County, Taiwan, Republic of China.
authors.forename: Mo-Chi
authors.initials: MC
authors.lastname: Cheng
authors.forename: Chia-Ying
authors.initials: CY
authors.lastname: Li
authors.forename: Han-Chieh
authors.initials: HC
authors.lastname: Ko
authors.forename: Feng-Nien
authors.initials: FN
authors.lastname: Ko
authors.forename: Yun-Lian
authors.initials: YL
authors.lastname: Lin
authors.forename: Tian-Shung
authors.initials: TS
authors.lastname: Wu
mesh_terms: D000928:Antidepressive Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D003042:Cocaine / Q000031:analogs & derivatives / Q000494:pharmacology; D003891:Desipramine / Q000494:pharmacology; D015394:Molecular Structure; D050484:Norepinephrine Plasma Membrane Transport Proteins / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D031801:Polygala / Q000737:chemistry*; D013624:Taiwan
chemical_list: D000928:Antidepressive Agents; D050484:Norepinephrine Plasma Membrane Transport Proteins; D009844:Oligosaccharides; C069723:2beta-carbomethoxy-3beta-(4-iodophenyl)tropane; D003042:Cocaine; D003891:Desipramine