Glutamine gluconeogenesis in the small intestine of 72 h-fasted adult rats is undetectable.
Recent reports have indicated that 48-72 h of fasting, Type 1 diabetes and high-protein feeding induce gluconeogenesis in the small intestine of adult rats in vivo. Since this would (i) represent a dramatic revision of the prevailing view that only the liver and the kidneys are gluconeogenic and (ii) have major consequences in the metabolism, nutrition and diabetes fields, we have thoroughly re-examined this question in the situation reported to induce the highest rate of gluconeogenesis. For this, metabolically viable small intestinal segments from 72 h-fasted adult rats were incubated with [3-13C]glutamine as substrate. After incubation, substrate utilization and product accumulation were measured by enzymatic and NMR spectroscopic methods. Although the segments utilized [13C]glutamine at high rates and accumulated 13C-labelled products linearly for 30 min in vitro, no substantial glucose synthesis could be detected. This was not due to the re-utilization of [13C]glucose initially synthesized from [13C]glutamine. Arteriovenous metabolite concentration difference measurements across the portal vein-drained viscera of 72 h-fasted Wistar and Sprague-Dawley rats clearly indicated that glutamine, the main if not the only gluconeogenic precursor taken up, could not give rise to detectable glucose production in vivo. Therefore we challenge the view that the small intestine of the adult rat is a gluconeogenic organ.
Martin
Guy
G
Institut National de la Santé et de la Recherche Médicale, UMR 499, Animet, Faculté de Médecine RTH Laennec, Université Lyon 1, Rue G. Paradin, 69372 Lyon Cedex 08, France.
Ferrier
Bernard
B
Conjard
Agnès
A
Martin
Mireille
M
Nazaret
Rémi
R
Boghossian
Michelle
M
Saadé
Fadi
F
Mancuso
Claire
C
Durozard
Daniel
D
Baverel
Gabriel
G
D000409:Alanine / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D005215:Fasting / Q000378:metabolism*; D005943:Gluconeogenesis / Q000502:physiology*; D005944:Glucosamine / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D018698:Glutamic Acid / Q000097:blood; D005973:Glutamine / Q000097:blood / Q000378:metabolism*; D006593:Hexokinase / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism*; D008297:Male; D019906:Nuclear Magnetic Resonance, Biomolecular; D011169:Portal Vein; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
D001786:Blood Glucose; D005973:Glutamine; D018698:Glutamic Acid; D006593:Hexokinase; D005947:Glucose; D005944:Glucosamine; D000409:Alanine