[Study on the genotyping and microevolution of Yersinia pestis in the Qinghai-Tibet Plateau].
OBJECTIVE
To study the distribution of genomovars and microevolution of Yersinia pestis in the Qinghai-Tibet Plateau.


METHODS
Primer pairs targeting the twenty-two different regions(DFRs) were designed for detecting the presence or deletion of each DFR in 297 strains isolated from the Qinghai-Tibet Plateau.


RESULTS
9 genomovars, i. e. Genomovar 1, 5, 6, 7, 8, 10, 11, new type and Ype-ancestor were identified in the Marmota himalayana plague focus of the Qinghai-Tibet Plateau. Among these genomovars, genomovar 5,8 and 10 were dominant types. The total rate of the three genomovars was 80.6% (204/253) and the genomovars in different regions were different. All of 44 strains of Y. pestis in the Microtus fuscus plague focus of the Qinghai-Tibet Plateau belonged to genomovar 14.


CONCLUSIONS
The distribution of genomovars of Y. pestis in the Qinghai-Tibet plateau had remarkable characteristics geographically. Based on the distribution of genomovars of Y. pestis, the routes of transmission and microevolution of Y. pestis were proposed.
Li
Min
M
Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China.
Dai
Er-hei
EH
Dai
Rui-xia
RX
Zhou
Dong-sheng
DS
Yang
Xiao-yan
XY
Cui
Bai-zhong
BZ
Jin
Li-xia
LX
Zhao
Hai-hong
HH
Li
Cun-xiang
CX
Qi
Mei-ying
MY
Ci Ren
Dun-zhu
DZ
Dai
Xiang
X
Tang
Yong-jiao
YJ
Yang
Rui-fu
RF
D005075:Biological Evolution; D002681:China; D005843:Geography; D006801:Humans; D010930:Plague / Q000635:transmission*; D015010:Yersinia pestis / Q000235:genetics*