Indian mustard [Brassica juncea (L.) Czern.].
All economically important Brassica species have been successfully transformed using Agrobacterium tumefaciens. Although different tissues have been used as explants, hypocotyls remain the most desirable explants for Brassica tissue culture owing to their amenability to regeneration. Young explants excised from 3- to 4-d-old seedlings have exhibited optimal regeneration potential; the addition of adjuvants such as silver nitrate to the selection medium is necessary to achieve high efficiency of transformation. This chapter describes an Agrobacterium-mediated transformation protocol for Indian mustard based on inoculation of hypocotyls. The selectable marker gene used encodes for neomycin phosphotransferase II (nptII), and the selection agent is kanamycin.
Gasic
Ksenija
K
Department of Natural Resources & Environment Sciences, University of Illinois, Urbana, IL, USA.
Korban
Schuyler S
SS
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000254:growth & development; D004351:Drug Resistance / Q000187:drug effects / Q000235:genetics; D018014:Gene Transfer Techniques; D005819:Genetic Markers; D018546:Hypocotyl / Q000166:cytology / Q000235:genetics* / Q000382:microbiology; D009149:Mustard Plant / Q000166:cytology / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics* / Q000382:microbiology; D012835:Silver Nitrate / Q000494:pharmacology; D014170:Transformation, Genetic
D005819:Genetic Markers; D012835:Silver Nitrate