title: Identification of chromosome aberrations in esophageal cancer cell line KYSE180 by multicolor fluorescence in situ hybridization.
abstract: Analysis of chromosomal changes in esophageal squamous cell carcinoma (ESCC) can illuminate the molecular mechanisms underlying the development and progression of this cancer, which is among the 10 most common malignant tumors. Cell lines are better suited than surgical samples for chromosome analysis in this cancer. This study used multicolor fluorescence in situ hybridization (M-FISH) to characterize the molecular cytogenetics of ESCC in cell line KYSE180. Two pools of 12-color whole-chromosome painting probes were designed, and two rounds of FISH were performed on the same metaphase spreads. Loss of DNA copy number was observed at 4p, 5q, 6q, 9, 10p, 12p, 13, 14p, 15p, 18p, 18q, 20, 22, and Y. Chromosomal gains and translocations occurred at the entire or part of 1, 2p, 3, 4p, 5p, 5q, 6p, 7, 8, 10q, 11, 12q, 14q, 16, 17q, 19, and Xp. Seven derivative chromosomes (5, 8, 12, 14, 14, 14, and 17) presented complex translocations, each involving three or four chromosomes. No chromosomes 9, 13, or Y were detected. These results add significant information to the existing karyotype description of KYSE180 and provide detailed cytogenetic background data for appropriate use of the cell line.
authors.affiliation: National Laboratory of Molecular Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences and Peking Union Medical College, Chaoyang District, Beijing, China.
authors.forename: Yu-Peng
authors.initials: YP
authors.lastname: Wu
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Yang
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Yang
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Man-Li
authors.initials: ML
authors.lastname: Luo
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Feng
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Han
authors.forename: Yan
authors.initials: Y
authors.lastname: Cai
authors.forename: Qi-Min
authors.initials: QM
authors.lastname: Zhan
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Jin-Tang
authors.initials: JT
authors.lastname: Dong
authors.forename: Ming-Rong
authors.initials: MR
authors.lastname: Wang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D004938:Esophageal Neoplasms / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping