Excision and transposition of piggyBac transposable element in tobacco budworm embryos.
The TTAA-specific lepidopteran transposon piggyBac has already proved useful as a gene-transfer vector for efficient transformation of a wide variety of insects. Transposable element excision and transposition assays are useful indicators of an element's ability to be mobilized in vivo and, thus, potentially serve as a transforming vector. Here, we report that this transposon is capable of excision and transposition in tobacco budworm embryos with relatively low frequency.
Ren
Xiaoxia
X
Department of Entomology, University of California, Riverside, CA 92521, USA.
Han
Zhaojun
Z
Miller
Thomas A
TA
D000818:Animals; D004251:DNA Transposable Elements; D018014:Gene Transfer Techniques; D009036:Moths / Q000235:genetics*; D011679:Pupa / Q000235:genetics; D014170:Transformation, Genetic
D004251:DNA Transposable Elements