title: Optimization of nutritional requirements and feeding strategies for clavulanic acid production by Streptomyces clavuligerus.
abstract: The present work reports the nutritional requirements and environmental conditions for submerged culture of Streptomyces clavuligerus for clavulanic acid production using orthogonal matrix method (Taguchi L(16) design) and also fed-batch fermentation for clavulanic acid production by feeding glycerol, arginine and threonoine to the fermentation medium intermittently. Clavulanic acid production was increased by 18% with the span of feeding glycerol and reached a maximum at 1.30mg/ml with 120h glycerol feeding as compared to 1.10mg/ml in the control. The production also increased with the span of feeding amino acids and reached a maximum of 1.31 and 1.86mg/ml with feeding arginine and threonine, respectively in 120h. There was an overall increase of 18% and 9% in clavulanic acid production with arginine and threonine feeding as compared to the respective controls (1.10 and 1.70mg/ml, respectively).
authors.affiliation: Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400 019, India.
authors.forename: Parag S
authors.initials: PS
authors.lastname: Saudagar
authors.forename: Rekha S
authors.initials: RS
authors.lastname: Singhal
mesh_terms: D001120:Arginine / Q000378:metabolism; D001431:Bacteriological Techniques; D019818:Clavulanic Acid / Q000096:biosynthesis* / Q000737:chemistry; D003470:Culture Media; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism*; D013912:Threonine / Q000378:metabolism
chemical_list: D003470:Culture Media; D010710:Phosphates; D019818:Clavulanic Acid; D013912:Threonine; D001120:Arginine; D005990:Glycerol