title: On the influence of vector design on antibody phage display.
abstract: Phage display technology is an established technology particularly useful for the generation of monoclonal antibodies (mAbs). The isolation of phagemid-encoded mAb fragments depends on several features of a phage preparation. The aims of this study were to optimize phage display vectors, and to ascertain if different virion features can be optimized independently of each other. Comparisons were made between phagemid virions assembled by g3p-deficient helper phage, Hyperphage, Ex-phage or Phaberge, or corresponding g3p-sufficient helper phage, M13K07. All g3p-deficient helper phage provided a similar level of antibody display, significantly higher than that of M13K07. Hyperphage packaged virions at least 100-fold more efficiently than did Ex-phage or Phaberge. Phaberge's packaging efficiency improved by using a SupE strain. Different phagemids were also compared. Removal of a 56 base pair fragment from the promoter region resulted in increased display level and increased virion production. This critical fragment encodes a lacZ'-like peptide and is also present in other commonly used phagemids. Increasing display level did not show statistical correlation with phage production, phage infectivity or bacterial growth rate. However, phage production was positively correlated to phage infectivity. In summary, this study demonstrates simultaneously optimization of multiple and independent features of importance for phage selection.
authors.affiliation: Cangene Corporation, 3403 American Drive, Mississauga, Ontario L4V 1T4, Canada.
authors.forename: Glenn
authors.initials: G
authors.lastname: Soltes
authors.forename: Michael
authors.initials: M
authors.lastname: Hust
authors.forename: Kitty K Y
authors.initials: KK
authors.lastname: Ng
authors.forename: Aasthaa
authors.initials: A
authors.lastname: Bansal
authors.forename: Johnathan
authors.initials: J
authors.lastname: Field
authors.forename: Donald I H
authors.initials: DI
authors.lastname: Stewart
authors.forename: Stefan
authors.initials: S
authors.lastname: Dübel
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Cha
authors.forename: Erik J
authors.initials: EJ
authors.lastname: Wiersma
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D005822:Genetic Vectors; D007140:Immunoglobulin Fab Fragments / Q000235:genetics*; D008969:Molecular Sequence Data; D019151:Peptide Library; D014771:Virion / Q000235:genetics
chemical_list: D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library