title: Enantioseparation of palonosetron hydrochloride by micellar electrokinetic chromatography with sodium cholate as chiral selector.
abstract: The enantioseparation of four stereoisomers of palonosetron hydrochloride by micellar electrokinetic chromatography using sodium cholate as chiral surfactant was described. Sodium cholate was shown to be effective in separating palonosetron hydrochloride stereoisomers. For method optimization, several parameters such as sodium cholate concentration, buffer pH and concentration, the types and concentration of organic modifiers and applied voltage, on the enantioseparation were evaluated and the optimum conditions were obtained as follows: 30 mM borate buffer (pH 9.40) containing 70 mM sodium cholate and 20% (v/v) methanol with an applied voltage of 20 kV. Under these conditions, baseline separation of palonosetron hydrochloride stereoisomers was achieved within 18 min.
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Kan
authors.initials: K
authors.lastname: Tian
authors.forename: Hongli
authors.initials: H
authors.lastname: Chen
authors.forename: Jianghong
authors.initials: J
authors.lastname: Tang
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
authors.forename: Zhide
authors.initials: Z
authors.lastname: Hu
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D007546:Isoquinolines / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D000077924:Palonosetron; D011812:Quinuclidines / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D020358:Sodium Cholate / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D007546:Isoquinolines; D011812:Quinuclidines; D000077924:Palonosetron; D020358:Sodium Cholate