title: Scanning molecular sieve chromatography of interacting protein systems. III. Effect of kinetic parameters on the large zone boundary profiles for local equilibration between mobile and stationary phases.
abstract: Large zone reaction boundary profiles for molecular sieve chromatography as affected by kinetic parameters have been simulated for local equilibration between the mobile and stationary phases. Our studies of monomer-dimer and monomer-tetramer systems indicate that in a slowly equilibrating system, the kinetic controls operating between the mobile and stationary phases contribute most significantly to the overall boundary profile. In a rapidly equilibrating system, however, the kinetic parameters k(ij) and k(ji) operating in the mobile phase are the principal determinants of the reaction boundary, while the kinetic effects of k(ii) and k-(ii) between the mobile and stationary phases are minimal.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA.
authors.forename: P W
authors.initials: PW
authors.lastname: Chun
authors.forename: M C
authors.initials: MC
authors.lastname: Yang