title: Effect of simulated pulpal pressure on all-in-one adhesive bond strengths to dentine.
abstract: OBJECTIVE
  To evaluate the durability of all-in-one adhesive systems bonded to dentine with and without simulated hydrostatic pulpal pressure (PP).
  
  
  METHODS
  Flat dentine surfaces of extracted human molars were prepared. Two all-in-one adhesive systems, One-Up Bond F (OBF) (Tokuyama Corp., Tokyo, Japan), and Fluoro Bond Shake One (FBS) (Shofu Co., Kyoto, Japan) were applied to the dentine surfaces under either a PP of 0 or 15cm H(2)O. Then, resin composite build-ups were made. The specimens bonded under pressure were stored in 37 degrees C water for 24h, 1 and 3 months under 15cm H(2)O PP. Specimens not bonded under pressure were stored under zero PP. After storage, the specimens were sectioned into slabs that were trimmed to hourglass shapes and subjected to micro-tensile bond testing (muTBS). The data were analysed using two-way ANOVA and Holm-Sidak HSD multiple comparison tests (alpha=0.05).
  
  
  RESULTS
  The muTBS of OBF fell significantly (p<0.05) when PP was applied during bonding and storage, regardless of storage time. In contrast, although the muTBS of OBF specimens bonded and stored without hydrostatic pressure storage fell significantly over the 3 months period, the decrease was less than half as much as specimens stored under PP. In FBS bonded specimens, although there was no significant difference between the muTBS with and without hydrostatic pulpal pressure at 24h, by 1 and 3 months of storage under PP, significant reductions were seen compared with the control group without PP.
  
  
  CONCLUSIONS
  The muTBS of OBF bonded specimens was lowered more by simulated PP than by storage time; specimens bonded with FBS were not sensitive to storage time in the absence of PP, but showed lower bond strengths at 1 and 3 months in the presence of PP.
authors.affiliation: Cariology and Operative Dentistry, Department of Restorative Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. hosaka.ope@tmd.ac.jp
authors.forename: Keiichi
authors.initials: K
authors.lastname: Hosaka
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nakajima
authors.forename: Monica
authors.initials: M
authors.lastname: Yamauti
authors.forename: Juthatip
authors.initials: J
authors.lastname: Aksornmuang
authors.forename: Masaomi
authors.initials: M
authors.lastname: Ikeda
authors.forename: Richard M
authors.initials: RM
authors.lastname: Foxton
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.forename: Junji
authors.initials: J
authors.lastname: Tagami
mesh_terms: D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003782:Dental Pulp / Q000502:physiology*; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D006874:Hydrostatic Pressure; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D013314:Stress, Mechanical; D013499:Surface Properties; D013696:Temperature; D013718:Tensile Strength; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D003188:Composite Resins; D017220:Dentin-Bonding Agents; C503681:FL-BOND S-1; D008689:Methacrylates; C446260:One-Up-Bond F; D014867:Water