In vitro organogenesis and alkaloid accumulation in Datura innoxia.
The kinetics of tropane alkaloids accumulation in different organs such as roots, leaves, stems, flowers and seeds of Datura innoxia was investigated by GC-MS. Twenty-six tropane alkaloids were detected. The ester derivatives of tropine (3alpha-tigloyloxytropine and 3-tigloyloxy-6-hydroxytropine) are the major compounds. Undifferentiated callus were established from the stem explants of Datura innoxia using Murashige and Skoog (MS) medium supplied with 6-benzylaminopurine (BA, 1 mg l(-10) and indole-3-acetic acid (IAA, 0.5 mg l(-1)) in combination for 6 weeks. Callus differentiation was initiated by subculture onto solid MS medium, free from hormones, for more than 10 months. Initially, shoots were formed after four weeks from subculture. Further subculturing in basal MS medium without growth regulators initiated the rooting of a shooty callus after 6 weeks. Investigation of the alkaloid content of the unorganized and organized callus revealed that callus (either green or brown) yielded only trace amounts of alkaloids. On the other hand, re-differentiated shoots contained mainly scopolamine while re-differentiated roots biosynthesized hyoscyamine as the main alkaloid.
Zayed
Rawia
R
Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt. mrawiazayed@yahoo.com
Wink
Michael
M
El-Shamy
Hesham
H
D000470:Alkaloids / Q000737:chemistry / Q000378:metabolism*; D001593:Benzyl Compounds / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D032302:Datura / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D018525:Germination; D007210:Indoleacetic Acids / Q000737:chemistry / Q000378:metabolism; D018519:Meristem / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism; D018547:Plant Stems / Q000254:growth & development / Q000378:metabolism; D011687:Purines / Q000737:chemistry / Q000378:metabolism
D000470:Alkaloids; D001593:Benzyl Compounds; D007210:Indoleacetic Acids; D011687:Purines; C030737:indoleacetic acid; C480551:benzylaminopurine