title: Limitations of DNA sequencing for diagnosis of a mixed infection by two fungi, Phaeoacremonium venezuelense and a Plectophomella sp., in a transplant recipient.
abstract: We describe a rare case of a subcutaneous infection by both Phaeoacremonium venezuelense and Plectophomella sp. in a Brazilian male. Sequencing of a beta-tubulin gene fragment allowed us to confirm the identification of the former. However, a similar procedure of sequencing rRNA gene fragments was not useful for the identification of the latter fungus.
authors.affiliation: Unitat de Micologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, 43201 Reus, Spain. josep.guarro@urv.net
authors.forename: Josep
authors.initials: J
authors.lastname: Guarro
authors.forename: Agenor Messias
authors.initials: AM
authors.lastname: Silvestre
authors.forename: Gerard
authors.initials: G
authors.lastname: Verkley
authors.forename: Josep
authors.initials: J
authors.lastname: Cano
authors.forename: Olga Fischman
authors.initials: OF
authors.lastname: Gompertz
authors.forename: Josepa
authors.initials: J
authors.lastname: Gené
authors.forename: Marília Marufuji
authors.initials: MM
authors.lastname: Ogawa
authors.forename: Jane
authors.initials: J
authors.lastname: Tomimori-Yamashita
authors.forename: Solange Pistori
authors.initials: SP
authors.lastname: Teixeira
authors.forename: Fernando Augusto
authors.initials: FA
authors.lastname: de Almeida
mesh_terms: D000328:Adult; D001203:Ascomycota / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry*; D003881:Dermatomycoses / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D006801:Humans; D008297:Male; D003904:Mitosporic Fungi / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal