Insertion of two pyrene moieties into oligodeoxyribonucleotides for the efficient detection of deletion polymorphisms.
For the detection of deletion polymorphisms, two pyrene moieties are tethered to an oligodeoxyribonucleotide (ODN) on both sides of the intervening base; one- and two-base deletions can be selectively detected by the strength of the excimer emission.
Kashida
Hiromu
H
Research Center for Advanced Science and Technology, University of Tokyo, Komaba, Meguro-ku, Tokyo, 153-8904, Japan.
Asanuma
Hiroyuki
H
Komiyama
Makoto
M
D001483:Base Sequence; D004252:DNA Mutational Analysis / Q000379:methods*; D017353:Gene Deletion; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D011721:Pyrenes / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D011721:Pyrenes; C030984:pyrene