title: Altered sirtuin expression is associated with node-positive breast cancer.
abstract: Sirtuins are genes implicated in cellular and organismal ageing. Consequently, they are speculated to be involved in diseases of ageing including cancer. Various cancers with widely differing prognosis have been shown to have differing and characteristic expression of these genes; however, the relationship between sirtuin expression and cancer progression is unclear. In order to correlate cancer progression and sirtuin expression, we have assessed sirtuin expression as a function of primary cell ageing and compared sirtuin expression in normal, 'nonmalignant' breast biopsies to breast cancer biopsies using real-time polymerase chain reaction (PCR). Levels of SIRT7 expression were significantly increased in breast cancer (P<0.0001). Increased levels of SIRT3 and SIRT7 transcription were also associated with node-positive breast cancer (P<0.05 and P<0.0001, respectively). This study has demonstrated differential sirtuin expression between nonmalignant and malignant breast tissue, with consequent diagnostic and therapeutic implications.
authors.affiliation: Division of Cancer Sciences and Molecular Pathology, Department of Surgery, University of Glasgow, Western Infirmary Glasgow, 44 Church Street, Glasgow G11 6NT, UK.
authors.forename: N
authors.initials: N
authors.lastname: Ashraf
authors.forename: S
authors.initials: S
authors.lastname: Zino
authors.forename: A
authors.initials: A
authors.lastname: Macintyre
authors.forename: D
authors.initials: D
authors.lastname: Kingsmore
authors.forename: A P
authors.initials: AP
authors.lastname: Payne
authors.forename: W D
authors.initials: WD
authors.lastname: George
authors.forename: P G
authors.initials: PG
authors.lastname: Shiels
mesh_terms: D000704:Analysis of Variance; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000473:pathology*; D002460:Cell Line; D045744:Cell Line, Tumor; D016922:Cellular Senescence / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D024101:Mitochondrial Proteins / Q000235:genetics*; D011379:Prognosis; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D056566:Sirtuin 3; D037761:Sirtuins / Q000235:genetics*
chemical_list: C493956:CDKN1A protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D050759:Cyclin-Dependent Kinase Inhibitor p21; D024101:Mitochondrial Proteins; D012334:RNA, Neoplasm; C510661:SIRT7 protein, human; C466093:SIRT3 protein, human; D056566:Sirtuin 3; D037761:Sirtuins