title: Transport and activity of Desulfitobacterium dichloroeliminans strain DCA1 during bioaugmentation of 1,2-DCA-contaminated groundwater.
abstract: The transport and activity of Desulfitobacterium dichloroeliminans strain DCA1 in 1,2-dichloroethane (1,2-DCA)-contaminated groundwater have been evaluated through an in situ bioaugmentation test at an industrial site (Belgium). The migration of strain DCA1 was monitored from an injection well toward a monitoring well, and the effect of the imposed groundwater flow on its distribution was assessed by means of transport model MOCDENS3D. The results of the real-time PCR (16S rRNA gene) quantification downstream from the injection point were used to evaluate the bacterial distribution pattern simulated by MOCDENS3D. In the injection well, the 1,2-DCA concentration in the groundwater decreased from 939.8 to 0.9 microM in a 35 day time interval and in the presence of a sodium lactate solution. Moreover, analyses from the monitoring well showed that the cells were still active after transport through the aquifer, although biodegradation occurred to a lesser extent. This study showed that strain DCA1 can be successfully applied for the removal of 1,2-DCA under field conditions and that its limited retardation offers perspectives for large-scale cleanup processes of industrial sites.
authors.affiliation: Laboratory of Microbial Ecology and Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
authors.forename: Ann
authors.initials: A
authors.lastname: Maes
authors.forename: Hilde
authors.initials: H
authors.lastname: Van Raemdonck
authors.forename: Katherine
authors.initials: K
authors.lastname: Smith
authors.forename: Wendy
authors.initials: W
authors.lastname: Ossieur
authors.forename: Luc
authors.initials: L
authors.lastname: Lebbe
authors.forename: Willy
authors.initials: W
authors.lastname: Verstraete
mesh_terms: D002849:Chromatography, Gas; D045858:Desulfitobacterium / Q000235:genetics / Q000378:metabolism*; D052918:Environmental Restoration and Remediation; D005025:Ethylene Dichlorides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D005025:Ethylene Dichlorides; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; C024565:ethylene dichloride