Molecular analysis of clinical isolates of Mycobacterium bovis recovered from humans in Italy.
In order to achieve a better knowledge of Mycobacterium bovis epidemiology in Italy, 42 clinical isolates from humans were genotyped. Predominant molecular patterns were found in one cluster of 15 isolates sharing spoligotype (ST482), variable-number tandem repeat (VNTR), and IS6110-based restriction fragment length polymorphism (one 1.9-kb band) profiles and in two clusters of 6 and 3 Mycobacterium bovis BCG isolates differing by one VNTR character. The remaining 18 isolates yielded unique profiles. Our results confirm the potential utility of spoligotyping and VNTR typing as a major typing system of M. bovis isolates.
Lari
Nicoletta
N
Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Via San Zeno, 35/39 - I-56127 Pisa, Italy.
Rindi
Laura
L
Bonanni
Daniela
D
Tortoli
Enrico
E
Garzelli
Carlo
C
D002851:Chromatography, High Pressure Liquid; D004251:DNA Transposable Elements; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length
D004251:DNA Transposable Elements