title: The use of siRNA to validate immunofluorescence studies.
abstract: Cellular immunofluorescence studies can be validated by using either specific small interfering RNA (siRNA) duplexes or expression plasmids that induce the expression of specific siRNAs. The usage of either siRNA tool reduces the expression of the specific protein being studied, thus reducing substantially or abolishing the immunofluorescence detected when using a fluorescent antibody that recognizes the protein.
authors.affiliation: Serologicals Corporation, Charlottesville, VA, USA.
authors.forename: K Gregory
authors.initials: KG
authors.lastname: Moore
authors.forename: Wayne
authors.initials: W
authors.lastname: Speckmann
authors.forename: Ronald P
authors.initials: RP
authors.lastname: Herzig
mesh_terms: D005455:Fluorescent Antibody Technique / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids / Q000235:genetics; D034741:RNA, Small Interfering / Q000378:metabolism*; D015203:Reproducibility of Results; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering