title: Automated cell plating and sample treatments for fixed cells in high content assays.
abstract: Robust and reliable methods for the manipulation of neural cell lines, by passaging, plating, dye labeling, imaging, fixation, and immunocytochemistry, are required to enable consistent, reproducible screens to be performed. We describe herein procedures and processes we have established to maximize the level of consistency of cell plating, fixation, and dye or antibody labeling, to ensure that assays which we are running on a routine basis remain consistent across long periods of time. These procedures involve a variety of fully or semiautomated steps, using high-quality commercially available liquid handling and dispensing technology.
authors.affiliation: The Neuroscience Research Centre, Merck Sharp and Dohme Research Laboratories, Essex, UK.
authors.forename: Gillian R
authors.initials: GR
authors.lastname: Richards
authors.forename: Julie E
authors.initials: JE
authors.lastname: Kerby
authors.forename: Grace K Y
authors.initials: GK
authors.lastname: Chan
authors.forename: Peter B
authors.initials: PB
authors.lastname: Simpson
mesh_terms: D001322:Autoanalysis / Q000379:methods; D006801:Humans; D046888:Tissue Array Analysis / Q000379:methods*; D016707:Tissue Fixation