title: Construction of a reporter vector for the analysis of Bifidobacterium longum promoters.
abstract: In order to initiate studies on promoter activities in Bifidobacterium longum and to independently confirm transcriptional data generated by microarray experiments, we have constructed a versatile reporter plasmid based on a B. longum cryptic plasmid and the Escherichia coli gusA gene. The resulting plasmid, pMDY23, has been tested using three B. longum promoters.
authors.affiliation: Department of Nutrition and Health, Nestlé Research Center, P.O. Box 44, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Klijn
authors.forename: D
authors.initials: D
authors.lastname: Moine
authors.forename: M
authors.initials: M
authors.lastname: Delley
authors.forename: A
authors.initials: A
authors.lastname: Mercenier
authors.forename: F
authors.initials: F
authors.lastname: Arigoni
authors.forename: R D
authors.initials: RD
authors.lastname: Pridmore
mesh_terms: D001483:Base Sequence; D001644:Bifidobacterium / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D005966:Glucuronidase