title: Antioxidant and cancer cell proliferation inhibition effect of citrus pectin-oligosaccharide prepared by irradiation.
abstract: Pectin was dissolved in deionized distilled water (2%, vol/vol) and irradiated at 20 kGy using a Co-60 gamma ray irradiator. The resulting solution was dialyzed and lyophilized. The samples were separated into three groups to estimate their antioxidant and cancer cell proliferation effects: non-irradiated (0 kGy), irradiated (20 kGy), and dialyzed (20 kGy-F, mol wt <10,000) samples. Antioxidant properties of each treatment was tested by a beta-carotene-linoleic acid bleaching assay and electron donating ability and compared for antioxidant index, which indicated that the activity was higher in the order of 20 kGy-F > 20 kGy > 0 kGy. Spleen cell survival effect of the irradiated pectin (20 kGy) and dialyzed (20 kGy-F) samples was higher than the non-irradiated control (0 kGy). The pectins inhibited growth of the cancer cell in the order of 20 kGy- F > 20 kGy > 0 kGy. The Ames test revealed that none of the fractions was mutagenic, and there was no indication of a dose-dependent response for any of the samples. These results suggest that a functional pectin oligosaccharide can be produced by irradiation for the food industry without any chemical treatment.
authors.affiliation: Department of Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, KAERI, Jeongeup, Republic of Korea.
authors.forename: Ho Jin
authors.initials: HJ
authors.lastname: Kang
authors.forename: Cheorun
authors.initials: C
authors.lastname: Jo
authors.forename: Joong Ho
authors.initials: JH
authors.lastname: Kwon
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Son
authors.forename: Bong Jeon
authors.initials: BJ
authors.lastname: An
authors.forename: Myung Woo
authors.initials: MW
authors.lastname: Byun
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D002957:Citrus / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D005720:Gamma Rays; D006801:Humans; D019787:Linoleic Acid / Q000737:chemistry / Q000494:pharmacology; D009152:Mutagenicity Tests; D009369:Neoplasms / Q000473:pathology*; D009844:Oligosaccharides / Q000494:pharmacology / Q000528:radiation effects; D010084:Oxidation-Reduction; D010368:Pectins / Q000494:pharmacology* / Q000528:radiation effects*; D010851:Picrates; D019207:beta Carotene / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009844:Oligosaccharides; D010851:Picrates; D019207:beta Carotene; D010368:Pectins; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl