The RPS adeno detector for diagnosing adenoviral conjunctivitis.
OBJECTIVE
To compare the sensitivity, specificity, and accuracy of the RPS Adeno Detector (Rapid Pathogen Screening Inc., South Williamsport, PA) against both viral cell culture with confirmatory immunofluorescence staining (CC-IFA) and the polymerase chain reaction (PCR) for diagnosing adenoviral conjunctivitis.


METHODS
Prospective, nonrandomized, masked, multicenter clinical trial.


METHODS
One hundred eighty-six consecutive patients from 5 clinical centers seeking treatment within 1 week of developing a red eye and thought to have acute conjunctivitis.


METHODS
The RPS Adeno Detector is a 10-minute in-office lateral flow immunoassay. Patients were tested with the RPS Adeno Detector, CC-IFA, and PCR to detect the presence of adenovirus.


METHODS
The sensitivity, specificity, and accuracy of the RPS Adeno Detector were assessed for identifying cases of adenoviral conjunctivitis.


RESULTS
Compared with CC-IFA, the RPS Adeno Detector was 88% sensitive and 91% specific at detecting adenoviral conjunctivitis. Using PCR as a reference method, the sensitivity of the RPS Adeno Detector increased to 89% and the specificity increased to 94%. Compared with PCR, CC-IFA was found to be 91% as sensitive and 100% as specific.


CONCLUSIONS
The RPS Adeno Detector demonstrated sufficient sensitivity and specificity to be used in the physician's office for the detection of adenoviral conjunctivitis.
Sambursky
Robert
R
Manatee Sarasota Eye Clinic & Laser Center, Bradenton, Florida, USA. robsambo@yahoo.com
Tauber
Shachar
S
Schirra
Frank
F
Kozich
Kristian
K
Davidson
Richard
R
Cohen
Elisabeth J
EJ
D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000175:diagnosis* / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D003236:Conjunctivitis, Viral / Q000175:diagnosis* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D007223:Infant; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013666:Tears / Q000821:virology; D014776:Virus Cultivation
D000956:Antigens, Viral; D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic