Semax, an analog of ACTH(4-10) with cognitive effects, regulates BDNF and trkB expression in the rat hippocampus.
The heptapeptide Semax (Met-Glu-His-Phe-Pro-Gly-Pro) is an analog of the adrenocorticotropin fragment (4-10) which after intranasal application has profound effects on learning and exerts marked neuroprotective activities. Here, we found that a single application of Semax (50 microg/kg body weight) results in a maximal 1.4-fold increase of BDNF protein levels accompanying with 1.6-fold increase of trkB tyrosine phosporylation levels, and a 3-fold and a 2-fold increase of exon III BDNF and trkB mRNA levels, respectively, in the rat hippocampus. Semax-treated animals showed a distinct increase in the number of conditioned avoidance reactions. We suggest that Semax affects cognitive brain functions by modulating the expression and the activation of the hippocampal BDNF/trkB system.
Dolotov
Oleg V
OV
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia. oledg@img.ras.ru
Karpenko
Ekaterina A
EA
Inozemtseva
Lyudmila S
LS
Seredenina
Tamara S
TS
Levitskaya
Natalia G
NG
Rozyczka
Joanna
J
Dubynina
Elena V
EV
Novosadova
Ekaterina V
EV
Andreeva
Lyudmila A
LA
Alfeeva
Lyudmila Yu
LY
Kamensky
Andrey A
AA
Grivennikov
Igor A
IA
Myasoedov
Nikolay F
NF
Engele
Jürgen
J
D000281:Administration, Intranasal; D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D019208:Brain-Derived Neurotrophic Factor / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D003071:Cognition / Q000187:drug effects / Q000502:physiology; D003213:Conditioning, Psychological / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005091:Exons / Q000187:drug effects / Q000235:genetics; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D018697:Nootropic Agents / Q000494:pharmacology; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D020813:Receptor, trkB / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D019208:Brain-Derived Neurotrophic Factor; D018697:Nootropic Agents; D010446:Peptide Fragments; D012333:RNA, Messenger; C048487:ACTH (4-7), Pro-Gly-Pro-; D000324:Adrenocorticotropic Hormone; D020813:Receptor, trkB; C011394:ACTH (4-10)