title: Optimization of cutaneous electrically mediated plasmid DNA delivery using novel electrode.
abstract: The easy accessibility of skin makes it an excellent target for gene transfer protocols. To take advantage of skin as a target for gene transfer, it is important to establish an efficient and reproducible delivery system. Electroporation is an established technique for enhancing plasmid delivery to many tissues in vivo. A critical component of this technique is the electrode configuration. Electroporation parameters were optimized for transgene expression with minimal tissue damage with a novel electrode. The highest transgene expression and efficiency of individual cell transformation with minimal damage was produced with eight 150 ms pulses at field strength of 100 V/cm. This electrode design offers the potential for easier and more reproducible electrically mediated cutaneous plasmid delivery than the simple electrodes currently commercially available. This electrode can be a valuable tool in determining the applicability of electrically mediated cutaneous gene transfer.
authors.affiliation: Center for Molecular Delivery, University of South Florida, Tampa, FL 33612, USA. rheller@hsc.usf.edu
authors.forename: L C
authors.initials: LC
authors.lastname: Heller
authors.forename: M J
authors.initials: MJ
authors.lastname: Jaroszeski
authors.forename: D
authors.initials: D
authors.lastname: Coppola
authors.forename: A N
authors.initials: AN
authors.lastname: McCray
authors.forename: J
authors.initials: J
authors.lastname: Hickey
authors.forename: R
authors.initials: R
authors.lastname: Heller
mesh_terms: D000818:Animals; D004566:Electrodes; D018274:Electroporation / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005260:Female; D015870:Gene Expression; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009336:Necrosis; D010957:Plasmids / Q000008:administration & dosage*; D012867:Skin / Q000201:enzymology* / Q000473:pathology
chemical_list: D008156:Luciferases