title: Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography.
abstract: An isocratic, reversed-phase HPLC assay has been developed for the separation of the enantiomers of four lipoxygenase metabolites, without the need for a derivatization step. Separation of the enantiomers was studied on a polysaccharide type chiral stationary phase column. Upon determination of suitable mobile phase composition, the assay was evaluated at various temperatures. In all cases the R enantiomer eluted before the S enantiomer. The best separations were observed at 0 degrees C.
authors.affiliation: University of Arizona, College of Pharmacy, 1703 East Mabel Street, Tucson, AZ 85721, USA. myrdal@pharmacy.arizona.edu <myrdal@pharmacy.arizona.edu>
authors.forename: Paul B
authors.initials: PB
authors.lastname: Myrdal
authors.forename: B Steven
authors.initials: BS
authors.lastname: Angersbach
authors.forename: Kelly
authors.initials: K
authors.lastname: Karlage
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Kuehl
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008084:Lipoxygenase / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D015203:Reproducibility of Results; D013237:Stereoisomerism
chemical_list: D008084:Lipoxygenase