Kinetics of reversible protein denaturation. A study on aplysia myoglobin.
The kinetics of denaturation and renaturation of Aplysia Myoglobin by temperature-jump and stopped-flow are reported. The time course of the unfolding and refolding reveals the presence of significant amounts of intermediates both in absence and in the presence of alcohols. A linear three states reaction mechanism is proposed and discussed.
Giacometti
G M
GM
CNR Center of Molecular Biology and Institute of Chemistry, Faculty of Medicine, University of Rome, Rome, Italy.
Antonini
E
E
Brunori
M
M