A comparison of reproductive characteristics of boars generated by somatic cell nuclear transfer to highly related conventionally produced boars.
This study compares the reproductive performance of boars produced by somatic cell nuclear transfer versus conventional breeding. Two different genotypes were selected for comparison: terminal cross line 1 (TX1) and terminal cross line 2 (TX2). The boars selected for comparison from TX1 were three cloned boars, produced by somatic cell nuclear transfer and the conventionally produced progenitor of the clones. The boars selected for comparison from TX2 were a cloned boar produced by somatic cell nuclear transfer and two conventionally produced half sibling boars that were offspring of the progenitor of the clone. Semen from each boar was collected, extended, evaluated and shipped offsite. Upon arrival, the semen was reevaluated and utilized for artificial insemination of 89 commercial gilts, at least 12 gilts per boar, producing 625 piglets. Pregnancy rates were determined at day 30 and 110 of gestation; and farrowing rate and gestation length were recorded. Differences were observed in some of the semen characteristics analyzed with the clones usually possessing superior semen quality to the control, this likely being a result of age differences amongst the clones and controls. Additionally no differences were noted between the clones and controls (progenitor) or between individual boars within genetic line for pregnancy rates, gestation length or any of the litter parameters examined between the clones and controls. These data further support previous reports with limited numbers that the reproductive capabilities of cloned boars are equal to that of conventionally produced boars.
Williams
N E
NE
ViaGen, Inc., Austin, Texas 78727, USA.
Walker
S C
SC
Reeves
D E
DE
Sherrer
E
E
Galvin
J M
JM
Polejaeva
I
I
Rampacek
G
G
Benyshek
L
L
Christenson
R K
RK
Graves
W M
WM
Pratt
S L
SL
D000818:Animals; D001947:Breeding; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D005260:Female; D005298:Fertility / Q000502:physiology; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D012098:Reproduction / Q000502:physiology; D034421:Sus scrofa / Q000502:physiology*