Spin-labelling study of interactions of ovalbumin with multilamellar liposomes and specific anti-ovalbumin antibodies.
Ovalbumin (OVA) has been used continuously as the model antigen in numerous studies of immune reactions and antigen processing, very often encapsulated into liposomes. The purpose of this work was to study the possible interactions of spin-labelled OVA and lipids in liposomal membranes using electron spin resonance (ESR) spectroscopy. OVA was covalently spin-labelled with 4-maleimido-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO-maleimide), characterized and encapsulated into multilamellar, negatively charged liposomes. ESR spectra of this liposomal preparation gave evidence for the interaction of OVA with the lipid bilayers. Such an interaction was also evidenced by the ESR spectra of liposomal preparation containing OVA, where liposomes were spin-labelled with n-doxyl stearic acids. The spin-labelled OVA retains its property to bind specific anti-OVA antibodies, as shown by ESR spectroscopy, but also in ELISA for specific anti-OVA IgG.
Brgles
Marija
M
Institute of Immunology Inc., PO Box 266, 10001 Zagreb, Croatia.
Mirosavljević
Krunoslav
K
Noethig-Laslo
Vesna
V
Frkanec
Ruza
R
Tomasić
Jelka
J
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002645:Chickens; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism*; D010047:Ovalbumin / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*; D010457:Peptidoglycan / Q000378:metabolism; D013113:Spin Labels; D013552:Swine; D013696:Temperature
D000906:Antibodies; D008081:Liposomes; D010457:Peptidoglycan; D013113:Spin Labels; D010047:Ovalbumin