title: Therapeutic efficacy of intra-cochlear administration of methylprednisolone after acoustic trauma caused by gunshot noise in guinea pigs.
abstract: The therapeutic efficacy of cochlear infusion of methylprednisolone (MP) after an impulse noise trauma (170dB SPL peak) was evaluated in guinea pigs. The compound action potential threshold shifts were measured over a 14 days recovery period after the gunshot exposure. For each animal, one of the cochlea was perfused directly into the scala tympani with MP during 7 days via a mini-osmotic pump, whereas the other cochlea was not pump-implanted. The functional study of hearing was supplemented by histological analysis. Forty eight hours after the trauma, significant differences between auditory threshold shifts in the implanted and non-implanted ears were observed for frequencies above 8kHz. At day 7, the difference was significant for only one frequency and no difference was observed after 14 days recovery. Cochleograms showed that the hair cell losses were significantly lower in the MP treated ears. This work indicates that direct infusion of MP into perilymphatic space accelerates hearing recovery, reduces hair cell losses after impulse noise trauma but does not limit permanent threshold shifts.
authors.affiliation: Centre de recherches du service de santé des armées (CRSSA), Département de radiobiologie, 24 avenue des Maquis du Grésivaudan, BP 87, 38702 La Tronche Cedex, France. isendowski@crssa.net
authors.forename: I
authors.initials: I
authors.lastname: Sendowski
authors.forename: L
authors.initials: L
authors.lastname: Abaamrane
authors.forename: F
authors.initials: F
authors.lastname: Raffin
authors.forename: A
authors.initials: A
authors.lastname: Cros
authors.forename: D
authors.initials: D
authors.lastname: Clarençon
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D003051:Cochlea / Q000187:drug effects*; D005389:Firearms; D006168:Guinea Pigs; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects / Q000473:pathology; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects / Q000473:pathology; D006317:Hearing Loss, Noise-Induced / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D008775:Methylprednisolone / Q000008:administration & dosage*; D016328:NF-kappa B / Q000502:physiology; D009569:Nitric Oxide / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D016328:NF-kappa B; D009569:Nitric Oxide; D008775:Methylprednisolone