Prime-boost strategies in DNA vaccines.
Induction of HIV-specific T-cell responses by vaccines may facilitate efficient control of HIV replication. Plasmid DNA vaccines and recombinant fowlpox virus (rFPV) vaccines are promising HIV-1 vaccine candidates, although delivering either vaccine alone may be insufficient to induce sufficient T-cell responses. A consecutive immunization strategy, known as "prime-boost," involving priming with DNA and boosting with rFPV vaccines encoding multiple common HIV antigens, is used to induce broad and high-level T-cell immunity and ameliorate AIDS in macaques. This vaccine strategy is proceeding to clinical trials. This chapter describes the use of prime-boost vaccines to induce T-cell responses against HIV-1 and protective immunity against AIDS in macaques. Methods for the construction of the vaccines, the use of animal models, and the detection of immune responses are described.
Dale
C Jane
CJ
Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia.
Thomson
Scott
S
De Rose
Robert
R
Ranasinghe
Charani
C
Medveczky
C Jill
CJ
Pamungkas
Joko
J
Boyle
David B
DB
Ramshaw
Ian A
IA
Kent
Stephen J
SJ
D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000517:prevention & control; D000818:Animals; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D005587:Fowlpox virus; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D007117:Immunization, Secondary / Q000379:methods; D008251:Macaca; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D014779:Virus Replication / Q000187:drug effects / Q000276:immunology*
D016915:AIDS Vaccines; D015488:HIV Antigens; D019444:Vaccines, DNA