title: [Study of highly pathogenic H5N1 influenza virus isolated from sick and dead birds in Western Siberia].
abstract: The mass destruction of domestic birds has been registered in July, 2005 in Novosibirsk region. Influenza virus H5N1 have been isolated from bodies of the lost birds on developing chicken embryos and identified by serological and molecular biological methods. M-gene and genes coding hemagglutinin and neurominidase were in part sequening. The phylogenetic analysis of hemagglutinin gene has shown, that the isolated viruses are forming a claster with strains, isolated from birds during outbreak of the bird's flu on lake Tsinghai (Qinghai) in China in 2005, in Japan in 2004, and also with H5N1 strains, isolated from the person and birds in the countries of Southeast Asia during ipizootia in 2003-2004. The site of the restriction which associated with pathogenicity of isolated avian influenza viruses H5 serogroup, corresponds to sequence of high pathogenic strains, circulating in the countries of Southeast Asia. The test for pathogenicity with use of chickens has confirmed, that researched strains were high pathogenic for birds.
authors.forename: G G
authors.initials: GG
authors.lastname: Onishchenko
authors.forename: A M
authors.initials: AM
authors.lastname: Shestopalov
authors.forename: V A
authors.initials: VA
authors.lastname: Ternovoĭ
authors.forename: V A
authors.initials: VA
authors.lastname: Evseenko
authors.forename: A G
authors.initials: AG
authors.lastname: Durymanov
authors.forename: Iu N
authors.initials: IuN
authors.lastname: Rassadkin
authors.forename: A V
authors.initials: AV
authors.lastname: Zaĭkovskaia
authors.forename: S I
authors.initials: SI
authors.lastname: Zolotykh
authors.forename: A K
authors.initials: AK
authors.lastname: Iurlov
authors.forename: V N
authors.initials: VN
authors.lastname: Mikheev
authors.forename: S V
authors.initials: SV
authors.lastname: Netesov
authors.forename: I G
authors.initials: IG
authors.lastname: Drozdov
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D002681:China; D005814:Genes, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D005585:Influenza in Birds / Q000453:epidemiology / Q000821:virology*; D007564:Japan; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics; D010802:Phylogeny; D015183:Restriction Mapping; D012299:Rimantadine / Q000494:pharmacology; D012800:Siberia / Q000453:epidemiology; D014763:Viral Matrix Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence / Q000235:genetics; D015047:Zoonoses
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C031540:M-protein, influenza virus; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C109963:hemagglutinin, avian influenza A virus; D012299:Rimantadine; D009439:Neuraminidase