title: Effect of lactobacilli on E. coli adhesion to Caco-2 cells in vitro.
abstract: Inhibitory effect of various lactobacilli against pathogenic strains of E. coli in model system Caco2 cells was determined by enumerating the number of adhering E. coli after pre-incubation (exclusion), post-incubation (displacement) or co-incubation (competition) with lactobacilli. Porcine E. coli strain F107 (F18ab, Stx2v) in the competition assay with porcine lactobacillus strain P10 gave bacterial counts 7.25 (log CFU per well); in the exclusion test it was only 7.05 while in displacement test it reached 7.29. The lowest E. coli counts adhering to Caco-2 cells were in exclusion assay (pre-incubation, Lactobacillus inoculated as the first). Pre-treatment of E. coli with our lactobacilli strains reduced the cultivable E. coli numbers.
authors.affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia. horosova@saske.sk
authors.forename: K
authors.initials: K
authors.lastname: Horosová
authors.forename: D
authors.initials: D
authors.lastname: Bujnáková
authors.forename: V
authors.initials: V
authors.lastname: Kmet
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D018938:Caco-2 Cells; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000502:physiology*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D007778:Lactobacillus / Q000378:metabolism*; D019936:Probiotics / Q000494:pharmacology*; D013552:Swine / Q000382:microbiology