New cell lines from Lymantria xylina (Lepidoptera: Lymantriidae): characterization and susceptibility to baculoviruses.
Four new cell lines, designated as NTU-LY-1 to -4, respectively, were established from the pupal tissues of Lymantria xylina Swinhoe (Lepidoptera: Lymantriidae). These cell lines have been cultured approximately 80 passages during 2 years in TNM-FH medium supplemented with 8% fetal bovine serum, at a constant temperature of 28 degrees C. Each line consists of three major morphological types: round cells, spindle-shaped cells, and giant cells. The characterization of these cell lines showed that they are different from previously established lines derived from related Lepidopteran species. All new lines were susceptible to the L. xylina multiple nucleopolyhedrovirus (LyxyMNPV) and appeared to have a good potential for studying this virus.
Wu
Chih-Yu
CY
Department of Entomology, National Taiwan University, Taipei, Taiwan, ROC.
Wang
Chung-Hsiung
CH
D000818:Animals; D002460:Cell Line / Q000166:cytology* / Q000821:virology*; D049109:Cell Proliferation; D009036:Moths / Q000145:classification / Q000166:cytology* / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000254:growth & development / Q000472:pathogenicity*; D011679:Pupa / Q000166:cytology / Q000254:growth & development; D014779:Virus Replication