The incorporation of nucleoside analogs by human immunodeficiency virus type 1 reverse transcriptase decreases in the presence of polyamines.
Nucleoside analogs (NAs) are an important class of anti-retroviral compounds used against human immunodeficiency virus (HIV). We have analyzed the potential effect of polyamines on the incorporation of NAs during DNA synthesis by HIV type-1 (HIV-1) reverse transcriptase (RT). The polyamines exert the ability to decrease the incorporation of various dideoxynucleoside triphosphates (ddATP, ddTTP or ddCTP) with both RNA/DNA and DNA/DNA substrates in the following order: spermine>spermidine>putrescine. The reduction is a sequence-independent effect, taking place at different sequence context. The results suggest that polyamines might affect the inhibition of reverse transcription by nucleoside analogs HIV-1 RT directed.
Bakhanashvili
Mary
M
Infectious Diseases Unit, Chaim Sheba Medical Center, Tel Hashomer, 52621 Ramat-Gan, Israel. bakhanus@yahoo.com
Rahav
Galia
G
D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry*; D015224:Dideoxynucleosides / Q000737:chemistry* / Q000494:pharmacology; D054303:HIV Reverse Transcriptase / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry* / Q000494:pharmacology; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D048348:Reverse Transcription / Q000187:drug effects
D018076:DNA, Complementary; D015224:Dideoxynucleosides; D011073:Polyamines; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase