[Inhibition of HBV replication and antigen expression by RNA interference against different targets].
OBJECTIVE
To observe the inhibition of HBV replication and antigen expression by RNA interference aimed at different parts of the HBV genome.


METHODS
Following the rules of shRNA expression vector design and construction, we constructed seven kinds of sequence specific vectors and two kinds of mutant shRNA expression ones. We then cotransfected those shRNA and HBV expression vectors into HepG2 cells using lipofectamine2000. The level of HBV replication was investigated using Southern blot and the antigen expression using ELISA.


RESULTS
The replication of HBV DNA was inhibited by many shRNAs, especially the ones against P1, S2, C2, S1 and X. The inhibition rate against P1 was as high as 95%. Results obtained with ELISA showed that the shRNAs targeting C2, C1 and S2 had high rates of inhibition to HBsAg.


CONCLUSIONS
The replication and antigen expression of HBV could be inhibited by shRNAs aimed at four different open read frames, and higher inhibition rates of HBV replication and surface antigen expression could be obtained by P1 and C2, respectively.
Zhang
Bing-qiang
BQ
Institute for Viral Hepatitis, Key Laboratory of Molecular Biology of Infectious Diseases of the Ministry of Education, Chongqing University of Medical Sciences, Chongqing 400016, China.
Huang
Ying
Y
Tang
Ni
N
Wu
Ying
Y
Zhang
Jun
J
Chen
Wei-xian
WX
He
Tong-chuan
TC
Huang
Ai-long
AL
D015870:Gene Expression; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection; D014779:Virus Replication
D034741:RNA, Small Interfering