title: Extracellular pH alkalinization by Cl-/HCO3- exchanger is crucial for TASK2 activation by hypotonic shock in proximal cell lines from mouse kidney.
abstract: We have previously shown that K(+)-selective TASK2 channels and swelling-activated Cl(-) currents are involved in a regulatory volume decrease (RVD; Barriere H, Belfodil R, Rubera I, Tauc M, Lesage F, Poujeol C, Guy N, Barhanin J, Poujeol P. J Gen Physiol 122: 177-190, 2003; Belfodil R, Barriere H, Rubera I, Tauc M, Poujeol C, Bidet M, Poujeol P. Am J Physiol Renal Physiol 284: F812-F828, 2003). The aim of this study was to determine the mechanism responsible for the activation of TASK2 channels during RVD in proximal cell lines from mouse kidney. For this purpose, the patch-clamp whole-cell technique was used to test the effect of pH and the buffering capacity of external bath on Cl(-) and K(+) currents during hypotonic shock. In the presence of a high buffer concentration (30 mM HEPES), the cells did not undergo RVD and did not develop outward K(+) currents (TASK2). Interestingly, the hypotonic shock reduced the cytosolic pH (pH(i)) and increased the external pH (pH(e)) in wild-type but not in cftr (-/-) cells. The inhibitory effect of DIDS suggests that the acidification of pH(i) and the alkalinization of pH(e) induced by hypotonicity in wild-type cells could be due to an exit of HCO(3)(-). In conclusion, these results indicate that Cl(-) influx will be the driving force for HCO(3)(-) exit through the activation of the Cl(-)/HCO(3)(-) exchanger. This efflux of HCO(3)(-) then alkalinizes pH(e), which in turn activates TASK2 channels.
authors.affiliation: UMR Centre National de la Recherche Scientifique 6548, Université de Nice-Sophia Antipolis, 06108 Nice Cedex 2, France.
authors.forename: S
authors.initials: S
authors.lastname: L'Hoste
authors.forename: H
authors.initials: H
authors.lastname: Barriere
authors.forename: R
authors.initials: R
authors.lastname: Belfodil
authors.forename: I
authors.initials: I
authors.lastname: Rubera
authors.forename: C
authors.initials: C
authors.lastname: Duranton
authors.forename: M
authors.initials: M
authors.lastname: Tauc
authors.forename: C
authors.initials: C
authors.lastname: Poujeol
authors.forename: J
authors.initials: J
authors.lastname: Barhanin
authors.forename: P
authors.initials: P
authors.lastname: Poujeol
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D002021:Buffers; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D048429:Cell Size / Q000187:drug effects; D018118:Chloride Channels / Q000502:physiology; D027963:Chloride-Bicarbonate Antiporters / Q000502:physiology*; D002712:Chlorides / Q000494:pharmacology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007038:Hypotonic Solutions / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D009579:Nitrobenzoates / Q000494:pharmacology; D015221:Potassium Channels / Q000502:physiology; D024683:Potassium Channels, Tandem Pore Domain / Q000502:physiology*; D012964:Sodium / Q000494:pharmacology
chemical_list: D002021:Buffers; D018118:Chloride Channels; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides; D007038:Hypotonic Solutions; C498739:Kcnk5 protein, mouse; D009579:Nitrobenzoates; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D012964:Sodium; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid