Transport and activity of Desulfitobacterium dichloroeliminans strain DCA1 during bioaugmentation of 1,2-DCA-contaminated groundwater.
The transport and activity of Desulfitobacterium dichloroeliminans strain DCA1 in 1,2-dichloroethane (1,2-DCA)-contaminated groundwater have been evaluated through an in situ bioaugmentation test at an industrial site (Belgium). The migration of strain DCA1 was monitored from an injection well toward a monitoring well, and the effect of the imposed groundwater flow on its distribution was assessed by means of transport model MOCDENS3D. The results of the real-time PCR (16S rRNA gene) quantification downstream from the injection point were used to evaluate the bacterial distribution pattern simulated by MOCDENS3D. In the injection well, the 1,2-DCA concentration in the groundwater decreased from 939.8 to 0.9 microM in a 35 day time interval and in the presence of a sodium lactate solution. Moreover, analyses from the monitoring well showed that the cells were still active after transport through the aquifer, although biodegradation occurred to a lesser extent. This study showed that strain DCA1 can be successfully applied for the removal of 1,2-DCA under field conditions and that its limited retardation offers perspectives for large-scale cleanup processes of industrial sites.
Maes
Ann
A
Laboratory of Microbial Ecology and Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
Van Raemdonck
Hilde
H
Smith
Katherine
K
Ossieur
Wendy
W
Lebbe
Luc
L
Verstraete
Willy
W
D002849:Chromatography, Gas; D045858:Desulfitobacterium / Q000235:genetics / Q000378:metabolism*; D052918:Environmental Restoration and Remediation; D005025:Ethylene Dichlorides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D005025:Ethylene Dichlorides; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; C024565:ethylene dichloride