Distinct expression of Kaposi's sarcoma-associated herpesvirus-encoded proteins in Kaposi's sarcoma and multicentric Castleman's disease.
The expression of Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8)-encoded proteins is herein demonstrated in Kaposi's sarcoma (KS) and multicentric Castleman's disease (MCD) in a single lymph node derived from a patient with acquired immunodeficiency syndrome. Immunohistochemistry revealed that both lytic and latent KSHV proteins were expressed in cells of the MCD lesion. KSHV-encoded viral interleukin-6 was also detected in follicular dendritic cells of the germinal center. Cytoplasmic localization of open reading frame 59 protein and latency-associated nuclear antigen suggested KSHV activation in the MCD lesion. Moreover, a high copy number of KSHV was detected in the blood. Clinically, pegylated-liposomal doxorubicin induced regression of not only KS, but also lymphadenopathy of the MCD lesion with a decrease in KSHV load and human interleukin-6 in the blood. To the best of the authors' knowledge this is the first case demonstrating differential expression of virus proteins in two KSHV-associated diseases, KS and MCD, in the same section. The case confirms lytic KSHV infection in MCD, and suggests that clinical symptoms of MCD might be closely linked with KSHV activation.
Abe
Yasuhisa
Y
AIDS Clinical Center, Tokyo, Japan.
Matsubara
Daisuke
D
Gatanaga
Hiroyuki
H
Oka
Shinichi
S
Kimura
Satoshi
S
Sasao
Yuki
Y
Saitoh
Kiyoshi
K
Fujii
Takeshi
T
Sato
Yuko
Y
Sata
Tetsutaro
T
Katano
Harutaka
H
D000328:Adult; D000956:Antigens, Viral / Q000235:genetics; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D005871:Castleman Disease / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics; D012514:Sarcoma, Kaposi / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D000956:Antigens, Viral; D004273:DNA, Neoplasm; D004279:DNA, Viral; D015850:Interleukin-6; D009687:Nuclear Proteins; C110348:ORF59 protein, Human herpesvirus 8; D014764:Viral Proteins; C109540:latency-associated nuclear antigen