Transcriptional regulation of the human TR2 orphan receptor gene by nuclear factor 1-A.
The human testicular receptor 2 (TR2), a member of the nuclear hormone receptor superfamily, has no identified ligand yet. Previous evidence demonstrated that a 63bp DNA fragment, named the promoter activating cis-element (PACE), has been identified as a positive regulatory region in the 5' promoter region of the human TR2 gene. In the present report, the human nuclear factor 1-A (NF1-A) was identified as a transcriptional activator to recognize the center of the PACE, called the PACE-C. NF1-A could bind to the 18bp PACE-C region, and enhance about 13- to 17-fold of the luciferase reporter gene activity via the PACE-C in dose-dependent and orientation-independent manners. This transcriptional activation was further confirmed by real-time RT-PCR assay. In conclusion, our results indicated that NF1-A transcription factor plays an important role in the transcriptional activation of the TR2 gene expression via the PACE-C in the minimal promoter region.
Lin
Yun-Lu
YL
Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
Wang
Ya-Hui
YH
Lee
Han-Jung
HJ
D001483:Base Sequence; D023281:Genomics; D006801:Humans; D008969:Molecular Sequence Data; D051839:NFI Transcription Factors / Q000032:analysis / Q000378:metabolism*; D057107:Nuclear Receptor Subfamily 2, Group C, Member 1; D011988:Receptors, Thyroid Hormone / Q000096:biosynthesis / Q000235:genetics*; D020218:Response Elements; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation
D051839:NFI Transcription Factors; C497592:NFIA protein, human; C539822:NR2C1 protein, human; D057107:Nuclear Receptor Subfamily 2, Group C, Member 1; D011988:Receptors, Thyroid Hormone