[Protective effect of tanshinones against liver injury in mice loaded with restraint stress].
OBJECTIVE
To observe the protective effects of tanshinones (tanshinone IIA, tanshinone I, cryptotanshinone and dihydrotanshinone) against liver injury in mice loaded with restraint stress.


METHODS
The liver injury model was established under 12 h restraint stress in mice 5 days after tanshinones treatment. The hepatoprotective effects were evaluated by assessing alanine aminotransferase (ALT) levels in plasma. The contents of vitamin C, GSH and malondialdehyde (MDA) in liver were performed by HPLC and TBARS methods, respectively. Oxygen radical absorbance capacity (ORAC) assay was used to measure the antioxidant capacity.


RESULTS
Tanshinones decreased ALT and MDA levels, and increased ORAC, vitamin C and GSH levels in liver tissues as compared with restraint stress control. Tanshinones also significantly inhibited oxidation in vitro. Among four tanshinones, dihydrotanshinone was more effective than others both in vivo and in vitro test.


CONCLUSIONS
Tanshinones possesses potent antioxidant activity in vitro and in vivo, and protected against liver injury induced by restraint stress. The active mechanisms may be related to their antioxidant capability.
Xu
Jie-kun
JK
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Hiroshi
Kurihara
K
Zheng
Jie-jing
JJ
Jiang
Tao
T
Yao
Xin-sheng
XS
D045784:Abietanes; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000293:injuries / Q000378:metabolism; D008107:Liver Diseases / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012149:Restraint, Physical / Q000009:adverse effects; D027561:Salvia miltiorrhiza / Q000737:chemistry; D013312:Stress, Physiological / Q000150:complications*
D045784:Abietanes; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; D020011:Protective Agents; D017382:Reactive Oxygen Species; C021751:tanshinone; D008315:Malondialdehyde; C037886:cryptotanshinone; D000410:Alanine Transaminase; D005978:Glutathione; D001205:Ascorbic Acid