title: Catalytic core of alphavirus nonstructural protein nsP4 possesses terminal adenylyltransferase activity.
abstract: The RNA-dependent RNA polymerase nsP4 is an integral part of the alphavirus replication complex. To define the role of nsP4 in viral RNA replication and for a structure-function analysis, we expressed Sindbis virus nsP4 in Escherichia coli. The core catalytic domain of nsP4 (Delta97nsP4, a deletion of the N-terminal 97 amino acids), which consists of the predicted polymerase domain containing the GDD amino acid motif required for viral RNA synthesis, was stable against proteolytic degradation during expression. Therefore, the recombinant core domain and selected mutants were expressed and purified to homogeneity. We determined that Delta97nsP4 possesses terminal adenylyltransferase (TATase) activity, as it specifically catalyzed the addition of adenine to the 3' end of an acceptor RNA in the presence of divalent cations. Furthermore, Delta97nsP4 is unable to transfer other nucleotides (UTP, CTP, GTP, and dATP) to the acceptor RNA in the absence or presence of other nucleotides. Delta97nsP4 possessing a GDD-to-GAA mutation completely inactivates the enzymatic activity. However, a GDD-to-SNN mutation did not inactivate the enzyme but reduced its activity to approximately 45% of that of the wild type in the presence of Mg(2+). Investigation of the TATase of the GDD-to-SNN mutant revealed that it had TATase equivalent to that of the wild type in the presence of Mn(2+). Identification of Delta97nsP4 TATase activity suggests a novel function of the alphavirus RNA-dependent RNA polymerase in the maintenance and repair of the poly(A) tail, an element required for replication of the viral genome.
authors.affiliation: Department of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907-2054, USA.
authors.forename: Shailly
authors.initials: S
authors.lastname: Tomar
authors.forename: Richard W
authors.initials: RW
authors.lastname: Hardy
authors.forename: Janet L
authors.initials: JL
authors.lastname: Smith
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Kuhn
mesh_terms: D000225:Adenine / Q000378:metabolism; D019943:Amino Acid Substitution; D020134:Catalytic Domain / Q000235:genetics / Q000502:physiology*; D002413:Cations, Divalent / Q000494:pharmacology; D003001:Cloning, Molecular; D003067:Coenzymes / Q000494:pharmacology; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D020125:Mutation, Missense; D009713:Nucleotidyltransferases / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D012313:RNA / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017384:Sequence Deletion; D012845:Sindbis Virus; D013379:Substrate Specificity; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D002413:Cations, Divalent; D003067:Coenzymes; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; C499947:nsP4 protein, Sindbis virus; D012313:RNA; D009713:Nucleotidyltransferases; C020658:glutamine-synthetase adenylyltransferase; D000225:Adenine