Stably transformed insect cell lines: tools for expression of secreted and membrane-anchored proteins and high-throughput screening platforms for drug and insecticide discovery.
Insect cell-based expression systems are prominent amongst current expression platforms for their ability to express virtually all types of heterologous recombinant proteins. Stably transformed insect cell lines represent an attractive alternative to the baculovirus expression system, particularly for the production of secreted and membrane-anchored proteins. For this reason, transformed insect cell systems are receiving increased attention from the research community and the biotechnology industry. In this article, we review recent developments in the field of insect cell-based expression from two main perspectives, the production of secreted and membrane-anchored proteins and the establishment of novel methodological tools for the identification of bioactive compounds that can be used as research reagents and leads for new pharmaceuticals and insecticides.
Douris
Vassilis
V
Insect Molecular Genetics and Biotechnology Group, Institute of Biology National Centre for Scientific Research Demokritos, GR 153 10 Aghia Paraskevi Attikis (Athens), Greece.
Swevers
Luc
L
Labropoulou
Vassiliki
V
Andronopoulou
Evi
E
Georgoussi
Zafiroula
Z
Iatrou
Kostas
K
D000818:Animals; D002461:Cell Line, Transformed; D015195:Drug Design; D004742:Enhancer Elements, Genetic; D007313:Insecta / Q000235:genetics*; D007306:Insecticides; D008565:Membrane Proteins / Q000096:biosynthesis*; D026723:Polyadenylation; D011401:Promoter Regions, Genetic; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis*; D015534:Trans-Activators / Q000502:physiology
D007306:Insecticides; D008565:Membrane Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D043562:Receptors, G-Protein-Coupled; D011994:Recombinant Proteins; D015534:Trans-Activators