The enhanced monocyte adhesiveness after UVB exposure requires ROS and NF-kappaB signaling in human keratinocyte.
The infiltration of both monocyte and activated T cells in the skin is one of critical steps in the development of UVB-induced inflammation. Upregulation of adhesion molecules such as intercellular adhesion molecule 1 (ICAM-1) on the surface of keratinocytes plays an important role in this process. In this study, we examined the molecular mechanism responsible for UVB-induced expression of ICAM-1 and subsequent monocyte adhesion by keratinocyte. We observed that (1) UVB induced protein and mRNA expression of ICAM-1 in a dose- and time-dependent manner in human keratinocyte cell HaCaT; (2) UVB induced the translocation of NF-kappaB and inhibition of NF-kappaB by NF-kappaB inhibitors suppressed UVB-induced mRNA and protein expression of ICAM-1; (3) UVB increased the intracellular level of reactive oxygen species (ROS) by HaCaT cells; (4) UVB-induced increase of intracellular ROS level was suppressed by pretreatment with diphenyl iodonium (DPI) and N-acetyl cysteine (NAC); and (5) inhibition of UVB-induced ROS production by DPI or NAC suppressed UVB-mediated translocation of NF-kappaB, expression of ICAM-1 and subsequent monocyte adhesion in HaCaT cells. These results suggest that UVB-induced ROS is involved in the translocation of NF-kappaB which is responsible for expression of ICAM-1 and subsequent increased monocyte adhesion in human keratinocyte.
Park
Lee Jin
LJ
Department of Biomedical Science, Hallym University, Kangwon 200-702, Republic of Korea.
Ju
Sung Mi
SM
Song
Ha Yong
HY
Lee
Ji Ae
JA
Yang
Mi Young
MY
Kang
Young Hee
YH
Kwon
Hyung Joo
HJ
Kim
Tae-Yoon
TY
Choi
Soo Young
SY
Park
Jinseu
J
D002448:Cell Adhesion / Q000528:radiation effects; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000528:radiation effects*; D006801:Humans; D007249:Inflammation; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D009000:Monocytes / Q000276:immunology / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D012867:Skin / Q000276:immunology; D013601:T-Lymphocytes / Q000502:physiology; D014466:Ultraviolet Rays
D016328:NF-kappa B; D017382:Reactive Oxygen Species; D018799:Intercellular Adhesion Molecule-1