A Semliki Forest virus replicon vectored DNA vaccine expressing the E2 glycoprotein of classical swine fever virus protects pigs from lethal challenge.
Classical swine fever virus (CSFV) causes significant losses in pig industry in many countries in Asia and Europe. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. Recently, the replicon of alphaviruses, such as Semliki Forest virus (SFV), has been developed as replicative expression vectors for gene delivery. In this study, we constructed a plasmid DNA based on SFV replicon encoding the E2 glycoprotein of CSFV and evaluated its efficacy in rabbits and pigs. The results showed that the animals immunized with the DNA vaccine developed CSFV-specific neutralizing antibodies and were protected from virulent or lethal challenge. This demonstrates that the SFV replicon-derived DNA vaccine can be a potential marker vaccine against CSFV infections.
Li
Na
N
Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Harbin 150001, Heilongjiang, China.
Qiu
Hua-Ji
HJ
Zhao
Jian-Jun
JJ
Li
Yan
Y
Wang
Ming-Jie
MJ
Lu
Bao-Wei
BW
Han
Cheng-Gang
CG
Hou
Qiang
Q
Wang
Zhong-Hua
ZH
Gao
Hui
H
Peng
Wu-Ping
WP
Li
Guo-Xin
GX
Zhu
Qing-Hu
QH
Tong
Guang-Zhi
GZ
D000818:Animals; D006691:Classical Swine Fever / Q000276:immunology / Q000517:prevention & control*; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics; D011817:Rabbits; D012093:Replicon / Q000235:genetics; D012672:Semliki forest virus / Q000235:genetics*; D013552:Swine; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use
D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C087086:glycoprotein E2, classical swine fever virus