title: Establishment of an HPA-1- to -16-typed platelet donor registry in China.
abstract: In order to determine gene frequencies of human platelet antigen (HPA) and establish a panel of accredited HPA-1a, -2a, -4a, -5a and -6a-negative donors as well as an HPA-typed platelet donor registry, a total of 1000 Chinese donors of Han nationality (500 from north China and 500 from south China) were typed for HPA-1 through -16 using a DNA-based polymerase chain reaction with sequence-specific primers genotyping method. The gene frequencies of HPA-1b, -2b, -3b, -4b, -5b, -6bw, -10bw and -15b were 0.0060, 0.0485, 0.4055, 0.0045, 0.0140, 0.0135, 0.0005 and 0.4680, respectively. The HPA-7bw, -8bw, -9bw, -11bw, -12bw, -13bw, -14bw and -16bw alleles were not found. The HPA-2b and -5b homozygous donors were detected at low frequencies. The HPA mismatch probabilities potentially leading to alloimmunization in random platelet transfusion vary with a region from 0.1% to 37% depending on the distribution patterns of common and less common alleles in each system. This study provides a useful HPA-typed plateletpheresis donor registry in China and could improve platelet antibody detection and HPA-matched platelet transfusion in alloimmune thrombocytopenic patients.
authors.affiliation: Shanghai Blood Center, Shanghai, China. fengml@sh163.net
authors.forename: M L
authors.initials: ML
authors.lastname: Feng
authors.forename: D Z
authors.initials: DZ
authors.lastname: Liu
authors.forename: W
authors.initials: W
authors.lastname: Shen
authors.forename: J L
authors.initials: JL
authors.lastname: Wang
authors.forename: Z H
authors.initials: ZH
authors.lastname: Guo
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: K M
authors.initials: KM
authors.lastname: Du
authors.forename: K C
authors.initials: KC
authors.lastname: Qian
authors.forename: T M
authors.initials: TM
authors.lastname: Zhao
mesh_terms: D016824:Antigens, Human Platelet / Q000145:classification / Q000235:genetics*; D001782:Blood Donors / Q000600:supply & distribution; D002681:China / Q000208:ethnology; D005787:Gene Frequency / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D017713:Platelet Transfusion; D010983:Plateletpheresis; D012042:Registries
chemical_list: D016824:Antigens, Human Platelet