title: Selection of novel nickel-binding peptides from flagella displayed secondary peptide library.
abstract: Nickel (Ni) performs its biological or toxic functions in nickel-protein coordination form. Novel Ni-binding peptides were isolated from a random dodecapeptide library displayed on the flagella of Escherichia coli against immobilized ions. On the basis of isolated sequences rich in histidine residues, two secondary libraries were constructed respectively. By consequent selection, more Ni-chelating peptides were identified and the consensus motif RHXHR (where X was always H) was deduced. The result suggested that not only histidine, but also arginine, play an important role in Ni-binding. Furthermore, two selected clones (1035 and 2022) were chosen for further identification. They exhibited similar relative binding affinity, which was about nine times that of the original library derived clones and statistically much more significant than the positive control with polyhistidine insert. Free nickel ions could almost completely inhibit the binding of the clones 1035 and 2022 to immobilized nickel, implicating that the peptides were able to chelate nickel ions. These studies reveal that bacterial surface displayed peptide libraries may have promising future potential for the development of metal bioadsorbents. Furthermore, novel Ni-binding peptides may provide lead molecules for Ni-chelation and applications thereof.
authors.affiliation: Department of Biochemistry, Beijing Institute of Basic Medical Sciences, P.O. Box 130(3) Beijing 100850, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.forename: Chuan
authors.initials: C
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhong-Tao
authors.initials: ZT
authors.lastname: Xin
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.forename: Can-Quan
authors.initials: CQ
authors.lastname: Mao
authors.forename: Nong-Le
authors.initials: NL
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Ning-Sheng
authors.initials: NS
authors.lastname: Shao
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
authors.forename: Yan-Ning
authors.initials: YN
authors.lastname: Xue
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001667:Binding, Competitive; D017931:DNA Primers; D004926:Escherichia coli / Q000737:chemistry; D005407:Flagella / Q000737:chemistry*; D009532:Nickel / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D019151:Peptide Library; D010455:Peptides; D009532:Nickel