title: Proline induces alterations in nucleotide hydrolysis in rat blood serum.
abstract: The main objective of the present study was to evaluate the in vivo (acute and chronic) and in vitro effects of proline on serum nucleotide hydrolysis. For acute administration, 29-day-old rats received one subcutaneous injection of proline (18.2 (micromol/g body weight) or an equivalent volume of 0.9% saline solution (control) and were sacrificed 1 h, 3 h or 12 h later. Results showed that acute proline administration provoked a decrease in ATP (42%) and ADP (49%) hydrolysis when rats were sacrificed 1 h after the injection. Furthermore, in rats killed 3 h and 12 h after acute injection, no change in nucleotide hydrolysis were observed. For chronic treatment, buffered proline was injected subcutaneously twice a day at 10 h intervals from the 6(th) to the 28(th) day of age. Rats were sacrificed 3 h or 12 h after the last injection. Chronic administration of proline did not alter the nucleotide hydrolysis when the rats were killed 12 h after the last injection, but decreased ATP (15%) and ADP (32%) hydrolysis when rats were sacrificed 3 h after the last injection. The in vitro effect of proline (3.0 microM - 1.0 mM) on serum nucleotide hydrolysis was also investigated; results showed that 1.0 mM of proline significantly increased ATP (45%), ADP (55%) and AMP (49%) hydrolysis. The data indicate that proline in vivo and in vitro alters nucleotide hydrolysis, which may be involved in the pathogeny of hyperprolinemic patients.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, Porto Alegre, RS, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Delwing
authors.forename: Débora
authors.initials: D
authors.lastname: Delwing
authors.forename: João J F
authors.initials: JJ
authors.lastname: Sarkis
authors.forename: Angela T S
authors.initials: AT
authors.lastname: Wyse
mesh_terms: D000818:Animals; D006868:Hydrolysis / Q000187:drug effects; D008297:Male; D009711:Nucleotides / Q000097:blood* / Q000378:metabolism*; D011392:Proline / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009711:Nucleotides; D011392:Proline