Diet-dependent shifts in ruminal butyrate-producing bacteria.
The influence of a host's diet on Butyrivibrio and Pseudobutyrivibrio populations was investigated by competitive PCR. Specific primers were designed and competitive PCRs developed for both groups. Results (from 4 cows with different diets) suggested that high-fiber intake essentially increases the Butyrivibrio amounts in the rumen, whereas high-energy food additives lead to its suppression. The Pseudobutyrivibrio concentration also changed during the experiment but without any significant relation to the host's diet.
Mrázek
J
J
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Prague, Czechia.
Tepsic
K
K
Avgustin
G
G
Kopecný
J
J
D000821:Animal Feed; D000818:Animals; D002087:Butyrates / Q000378:metabolism*; D045856:Butyrivibrio / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D002417:Cattle; D003013:Clostridium / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis; D004043:Dietary Fiber; D016946:Gram-Negative Aerobic Rods and Cocci / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000145:classification / Q000235:genetics; D012417:Rumen / Q000382:microbiology*
D002087:Butyrates; D004269:DNA, Bacterial; D004043:Dietary Fiber; D012336:RNA, Ribosomal, 16S