Development of high-performance liquid chromatographic fingerprint for the quality control of Rheum tanguticum Maxim. ex Balf.
A novel, accurate and valid fingerprint method was developed by using high-performance liquid chromatography-photodiode array detection (HPLC-DAD) to control the quality of Rheum tanguticum Maxim. ex Balf. By comprehensively comparing chromatograms of all samples, our result revealed that the chromatographic fingerprint we developed combining similarity evaluation could efficiently identify and distinguish raw materials of R. tanguticum from different sources. Besides, 21 characteristic peaks in chromatogram of R. tanguticum were unambiguously confirmed by comparison of reference compounds based on their retention time (t(R)) and UV spectra.
Jin
Wei
W
School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, China.
Ge
Ri-Li
RL
Wei
Quan-Jia
QJ
Bao
Tian-You
TY
Shi
Hai-Ming
HM
Tu
Peng-Fei
PF
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D012250:Rheum / Q000737:chemistry*