Development of a fingerprint of Salvia miltiorrhiza Bunge by high-performance liquid chromatography with a coulometric electrode array system.
To standardize and control herbal medicines, a feasible approach and control system is necessary. In this paper, a high-performance liquid chromatography with a coulometric electrode array detector (HPLC-CEAD) system was applied to fingerprint Salvia miltiorrhiza Bunge (S. miltiorrhiza Bunge), a popular herbal medicine, for the first time. pH of mobile phase, working potentials and sample preparation were included in our research. Twenty-five common peaks were obtained from extracts of S. miltiorrhiza Bunge (Shandong province), more than that obtained in previous report. Fingerprints of S. miltiorrhiza Bunge from different locations were also studied. The content of main components varied in different samples. Overlapping ratio of peaks (ORP) in 10 batches of S. miltiorrhiza Bunge (Shandong province) was not less than 72.46%. In method validation, relative standard deviation (RSD) of relative retention times and relative peak areas were of not more than 3%. It was concluded that HPLC-CEAD system can be applied in fingerprinting herbal medicines.
Lijuan
Ma
M
Department of Biochemical Engineering School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Xuezhu
Zhang
Z
Haiping
Zhang
Z
Yiru
Gan
G
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry*