Rabbit embryonic stem cell lines derived from fertilized, parthenogenetic or somatic cell nuclear transfer embryos.
Embryonic stem cells were isolated from rabbit blastocysts derived from fertilization (conventional rbES cells), parthenogenesis (pES cells) and nuclear transfer (ntES cells), and propagated in a serum-free culture system. Rabbit ES (rbES) cells proliferated for a prolonged time in an undifferentiated state and maintained a normal karyotype. These cells grew in a monolayer with a high nuclear/cytoplasm ratio and contained a high level of alkaline phosphate activity. In addition, rbES cells expressed the pluripotent marker Oct-4, as well as EBAF2, FGF4, TDGF1, but not antigens recognized by antibodies against SSEA-1, SSEA-3, SSEA-4, TRA-1-10 and TRA-1-81. All 3 types of ES cells formed embryoid bodies and generated teratoma that contained tissue types of all three germ layers. rbES cells exhibited a high cloning efficiency, were genetically modified readily and were used as nuclear donors to generate a viable rabbit through somatic cell nuclear transfer. In combination with genetic engineering, the ES cell technology should facilitate the creation of new rabbit lines.
Fang
Zhen F
ZF
Center for Developmental Biology, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, 1665 Kong Jiang Road, Shanghai 200092, P.R. China.
Gai
Hui
H
Huang
You Z
YZ
Li
Shan G
SG
Chen
Xue J
XJ
Shi
Jian J
JJ
Wu
Li
L
Liu
Ailian
A
Xu
Ping
P
Sheng
Hui Z
HZ
D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000502:physiology; D002454:Cell Differentiation; D002460:Cell Line; D019976:Cloning, Organism / Q000379:methods*; D016895:Culture Media, Serum-Free; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D005306:Fertilization; D006801:Humans; D007621:Karyotyping; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D018895:Microsatellite Repeats; D053652:Nuclear Transfer Techniques; D010312:Parthenogenesis; D011817:Rabbits; D013724:Teratoma / Q000378:metabolism; D000097573:Red Fluorescent Protein
D016895:Culture Media, Serum-Free; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein