title: The enhanced monocyte adhesiveness after UVB exposure requires ROS and NF-kappaB signaling in human keratinocyte.
abstract: The infiltration of both monocyte and activated T cells in the skin is one of critical steps in the development of UVB-induced inflammation. Upregulation of adhesion molecules such as intercellular adhesion molecule 1 (ICAM-1) on the surface of keratinocytes plays an important role in this process. In this study, we examined the molecular mechanism responsible for UVB-induced expression of ICAM-1 and subsequent monocyte adhesion by keratinocyte. We observed that (1) UVB induced protein and mRNA expression of ICAM-1 in a dose- and time-dependent manner in human keratinocyte cell HaCaT; (2) UVB induced the translocation of NF-kappaB and inhibition of NF-kappaB by NF-kappaB inhibitors suppressed UVB-induced mRNA and protein expression of ICAM-1; (3) UVB increased the intracellular level of reactive oxygen species (ROS) by HaCaT cells; (4) UVB-induced increase of intracellular ROS level was suppressed by pretreatment with diphenyl iodonium (DPI) and N-acetyl cysteine (NAC); and (5) inhibition of UVB-induced ROS production by DPI or NAC suppressed UVB-mediated translocation of NF-kappaB, expression of ICAM-1 and subsequent monocyte adhesion in HaCaT cells. These results suggest that UVB-induced ROS is involved in the translocation of NF-kappaB which is responsible for expression of ICAM-1 and subsequent increased monocyte adhesion in human keratinocyte.
authors.affiliation: Department of Biomedical Science, Hallym University, Kangwon 200-702, Republic of Korea.
authors.forename: Lee Jin
authors.initials: LJ
authors.lastname: Park
authors.forename: Sung Mi
authors.initials: SM
authors.lastname: Ju
authors.forename: Ha Yong
authors.initials: HY
authors.lastname: Song
authors.forename: Ji Ae
authors.initials: JA
authors.lastname: Lee
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Yang
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Kang
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Tae-Yoon
authors.initials: TY
authors.lastname: Kim
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
mesh_terms: D002448:Cell Adhesion / Q000528:radiation effects; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000528:radiation effects*; D006801:Humans; D007249:Inflammation; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D009000:Monocytes / Q000276:immunology / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D012867:Skin / Q000276:immunology; D013601:T-Lymphocytes / Q000502:physiology; D014466:Ultraviolet Rays
chemical_list: D016328:NF-kappa B; D017382:Reactive Oxygen Species; D018799:Intercellular Adhesion Molecule-1