Enhancement of erythrocyte antioxidants by green and black tea polyphenols during 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis.
We evaluated the comparative chemopreventive efficacy of green tea polyphenols (polyphenon-E) and black tea polyphenols (polyphenon-B) on 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis. Lipid peroxidation, reduced and oxidized glutathione (GSH and GSSG, respectively), and the GSH-dependent enzymes glutathione peroxidase and glutathione S-transferase in the erythrocytes were used as biomarkers of chemoprevention. Enhanced lipid peroxidation in erythrocytes of DMBA-treated animals was accompanied by a significant decrease in the antioxidant status. Dietary administration of polyphenon-E and -B to DMBA-treated animals significantly decreased the extent of lipid peroxidation and enhanced the levels of GSH, GSH/GSSG ratio, and activities of GSH-dependent enzymes. Our study provides evidence that polyphenon-B is more effective in inhibiting HBP carcinogenesis than polyphenon-E by enhancing the antioxidant status, suggesting that polyphenon-B may have a major impact in the chemoprevention of oral cancer.
Chandra Mohan
K V P
KV
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India.
Subapriya
R
R
Hara
Y
Y
Nagini
S
S
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D002610:Cheek; D018890:Chemoprevention; D006224:Cricetinae; D004912:Erythrocytes / Q000737:chemistry*; D005419:Flavonoids / Q000008:administration & dosage*; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008647:Mesocricetus; D009062:Mouth Neoplasms / Q000097:blood* / Q000139:chemically induced; D010084:Oxidation-Reduction; D010636:Phenols / Q000008:administration & dosage*; D059808:Polyphenols; D013662:Tea / Q000737:chemistry*
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013662:Tea; D015127:9,10-Dimethyl-1,2-benzanthracene; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005978:Glutathione