title: Moxifloxacin but not ciprofloxacin or azithromycin selectively inhibits IL-8, IL-6, ERK1/2, JNK, and NF-kappaB activation in a cystic fibrosis epithelial cell line.
abstract: Cystic fibrosis (CF) is associated with severe neutrophilic airway inflammation. We showed that moxifloxacin (MXF) inhibits IL-8 and MAPK activation in monocytic and respiratory epithelial cells. Azithromycin (AZM) and ciprofloxacin (CIP) are used clinically in CF. Thus we now examined effects of MXF, CIP, and AZM directly on CF cells. IB3, a CF bronchial cell line, and corrected C38 cells were treated with TNF-alpha, IL-1beta, or LPS with or without 5-50 microg/ml MXF, CIP, or AZM. IL-6 and IL-8 secretion (ELISA), MAPKs ERK1/2, JNK, p38, and p65 NF-kappaB (Western blot) activation were measured. Baseline IL-6 was sixfold higher in IB3 than C38 cells but IL-8 was similar. TNF-alpha and IL-1beta increased IL-6 and IL-8 12- to 67-fold with higher levels in IB3 than C38 cells post-TNF-alpha (P < 0.05). Levels were unchanged following LPS. Baseline phosphorylated form of ERK1/2 (p-ERK1/2), JNK, and NF-kappaB p65 were higher in IB3 than C38 cells (5-, 1.4-, and 1.4-fold), and following TNF-alpha increased, as did the p-p38, by 1.6- to 2-fold. MXF (5-50 microg/ml) and CIP (50 microg/ml), but not AZM, suppressed IL-6 and IL-8 secretion by up to 69%. MXF inhibited TNF-alpha-stimulated MAPKs ERK1/2, 46-kDa JNK, and NF-kappaB up to 60%, 40%, and 40%, respectively. In contrast, MXF did not inhibit p38 activation, implying a highly selective pretranslational effect. In conclusion, TNF-alpha and IL-1beta induce an exaggerated inflammatory response in CF airway cells, inhibited by MXF more than by CIP or AZM. Clinical trials are recommended to assess efficacy in CF and other chronic lung diseases.
authors.affiliation: Schneider Children's Medical Center of Israel, 14 Kaplan Street, Petah Tikva, Israel 49202. hblau@post.tau.ac.il
authors.forename: Hannah
authors.initials: H
authors.lastname: Blau
authors.forename: Keren
authors.initials: K
authors.lastname: Klein
authors.forename: Itamar
authors.initials: I
authors.lastname: Shalit
authors.forename: Drora
authors.initials: D
authors.lastname: Halperin
authors.forename: Ina
authors.initials: I
authors.lastname: Fabian
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D001372:Aza Compounds / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology; D001980:Bronchi / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D002939:Ciprofloxacin / Q000494:pharmacology; D003550:Cystic Fibrosis / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors*; D024841:Fluoroquinolones; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D016209:Interleukin-8 / Q000037:antagonists & inhibitors*; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*; D008070:Lipopolysaccharides / Q000494:pharmacology; D000077266:Moxifloxacin; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D010766:Phosphorylation; D011804:Quinolines / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D000093242:Interleukin-6 Inhibitors; D015850:Interleukin-6
chemical_list: D000890:Anti-Infective Agents; D001372:Aza Compounds; D017963:Azithromycin; D002939:Ciprofloxacin; D048049:Extracellular Signal-Regulated MAP Kinases; D024841:Fluoroquinolones; D053583:Interleukin-1beta; D016209:Interleukin-8; D048031:JNK Mitogen-Activated Protein Kinases; D008070:Lipopolysaccharides; D000077266:Moxifloxacin; D016328:NF-kappa B; D011804:Quinolines; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors; C508600:IL6 protein, human; D015850:Interleukin-6