Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances.
To simplify the molecular detection of micro-organisms, we evaluated the tolerance of loop-mediated isothermal amplification (LAMP) to a culture medium and some biological substances. The sensitivity of LAMP was less affected by the various components of the clinical samples than was polymerase chain reaction (PCR); therefore, DNA purification from samples could be omitted.
Kaneko
Hisatoshi
H
Department of Microbiology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima, 960-1295, Japan.
Kawana
Takashi
T
Fukushima
Eiko
E
Suzutani
Tatsuo
T
D000818:Animals; D002522:Chlorocebus aethiops; D003470:Culture Media; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods* / Q000706:statistics & numerical data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods* / Q000706:statistics & numerical data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014709:Vero Cells
D003470:Culture Media; D004279:DNA, Viral