Efficacy of clone fingerprinting methodologies.
With the development of new high-information content fingerprinting techniques for constructing BAC-based physical maps, physical map construction is accelerating and it is important to determine which methodologies work best. In a recent publication (Z. Xu et al., 2004, Genomics 84:941-951), Xu et al. evaluated five different techniques (one agarose-based and four using multiple enzymes) and concluded that a two-enzyme technique was superior. In addition, they found that no benefit was gained from fingerprinting more than 10x coverage. In this paper we report our own extensive simulation results, which lead to contrasting conclusions. Our data indicate that the five-enzyme method known as SNaPshot is the most effective and that the assembly can in fact be significantly improved with greater than 10x coverage.
Nelson
William M
WM
Arizona Genomics Computational Laboratory, BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA.
Dvorak
Jan
J
Luo
Ming-Cheng
MC
Messing
Joachim
J
Wing
Rod A
RA
Soderlund
Carol
C
D017360:Arabidopsis / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics; D003001:Cloning, Molecular; D016172:DNA Fingerprinting / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics; D006801:Humans
D018744:DNA, Plant