Limitations of DNA sequencing for diagnosis of a mixed infection by two fungi, Phaeoacremonium venezuelense and a Plectophomella sp., in a transplant recipient.
We describe a rare case of a subcutaneous infection by both Phaeoacremonium venezuelense and Plectophomella sp. in a Brazilian male. Sequencing of a beta-tubulin gene fragment allowed us to confirm the identification of the former. However, a similar procedure of sequencing rRNA gene fragments was not useful for the identification of the latter fungus.
Guarro
Josep
J
Unitat de Micologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, 43201 Reus, Spain. josep.guarro@urv.net
Silvestre
Agenor Messias
AM
Verkley
Gerard
G
Cano
Josep
J
Gompertz
Olga Fischman
OF
Gené
Josepa
J
Ogawa
Marília Marufuji
MM
Tomimori-Yamashita
Jane
J
Teixeira
Solange Pistori
SP
de Almeida
Fernando Augusto
FA
D000328:Adult; D001203:Ascomycota / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry*; D003881:Dermatomycoses / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D006801:Humans; D008297:Male; D003904:Mitosporic Fungi / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal