title: A 1.5-Mb-resolution radiation hybrid map of the cat genome and comparative analysis with the canine and human genomes.
abstract: We report the construction of a 1.5-Mb-resolution radiation hybrid map of the domestic cat genome. This new map includes novel microsatellite loci and markers derived from the 2X genome sequence that target previous gaps in the feline-human comparative map. Ninety-six percent of the 1793 cat markers we mapped have identifiable orthologues in the canine and human genome sequences. The updated autosomal and X-chromosome comparative maps identify 152 cat-human and 134 cat-dog homologous synteny blocks. Comparative analysis shows the marked change in chromosomal evolution in the canid lineage relative to the felid lineage since divergence from their carnivoran ancestor. The canid lineage has a 30-fold difference in the number of interchromosomal rearrangements relative to felids, while the felid lineage has primarily undergone intrachromosomal rearrangements. We have also refined the pseudoautosomal region and boundary in the cat and show that it is markedly longer than those of human or mouse. This improved RH comparative map provides a useful tool to facilitate positional cloning studies in the feline model.
authors.affiliation: Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A & M University, Mail Stop 4458, College Station, TX 77843, USA. wmurphy@cvm.tamu.edu
authors.forename: William J
authors.initials: WJ
authors.lastname: Murphy
authors.forename: Brian
authors.initials: B
authors.lastname: Davis
authors.forename: Victor A
authors.initials: VA
authors.lastname: David
authors.forename: Richa
authors.initials: R
authors.lastname: Agarwala
authors.forename: Alejandro A
authors.initials: AA
authors.lastname: Schäffer
authors.forename: Alison J
authors.initials: AJ
authors.lastname: Pearks Wilkerson
authors.forename: Beena
authors.initials: B
authors.lastname: Neelam
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: O'Brien
authors.forename: Marilyn
authors.initials: M
authors.lastname: Menotti-Raymond
mesh_terms: D000818:Animals; D005075:Biological Evolution; D002415:Cats / Q000235:genetics*; D041321:Chromosomes, Human, X / Q000235:genetics; D004285:Dogs / Q000235:genetics; D005819:Genetic Markers; D016678:Genome; D015894:Genome, Human; D023281:Genomics; D006801:Humans; D018895:Microsatellite Repeats; D021101:Radiation Hybrid Mapping; D013045:Species Specificity; D014960:X Chromosome / Q000235:genetics
chemical_list: D005819:Genetic Markers