Mathematical modeling and computer simulation of isoelectric focusing with electrochemically defined ampholytes.
A mathematical model of isoelectric focusing at the steady state has been developed for an M-component system of electrochemically defined ampholytes. The model is formulated from fundamental principles describing the components' chemical equilibria, mass transfer resulting from diffusion and electromigration, and electroneutrality. The model consists of ordinary differential equations coupled with a system of algebraic equations. The model is implemented on a digital computer using FORTRAN-based simulation software. Computer simulation data are presented for several two-component systems showing the effects of varying the isoelectric points and dissociation constants of the constituents.
Palusinski
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Biophysics Technology Laboratory, University of Arizona, Tucson, AZ 85721, USA.
Allgyer
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Mosher
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Bier
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Saville
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