title: Coxsackievirus B3 in human milk.
abstract: Coxsackievirus B3 can cause severe neonatal disease with high mortality. We present the first report of detection of coxsackievirus B3 in the mothers' milk of 2 severely infected neonates by culture and reverse transcriptase-polymerase chain reaction. Reverse transcriptase-polymerase chain reaction is a rapid and sensitive tool to detect coxsackievirus B3 in breast milk within few hours. By 12 to 14 days after onset of symptoms, coxsackievirus B3 could not be detected in the breast milk of the symptomatic mothers.
authors.affiliation: Division of Neonatology, Department of Pediatrics, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan, Tiawan.
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Chang
authors.forename: Kuo-Chien
authors.initials: KC
authors.lastname: Tsao
authors.forename: Chi-Chia
authors.initials: CC
authors.lastname: Huang
authors.forename: Meng-Hsiu
authors.initials: MH
authors.lastname: Yen
authors.forename: Chung-Guei
authors.initials: CG
authors.lastname: Huang
authors.forename: Tzou-Yien
authors.initials: TY
authors.lastname: Lin
mesh_terms: D000328:Adult; D003384:Coxsackievirus Infections / Q000821:virology*; D029822:Enterovirus B, Human / Q000254:growth & development / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008895:Milk, Human / Q000821:virology*; D009035:Mothers; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D019562:Viral Load
chemical_list: D012367:RNA, Viral