gammaepsilon Sub-complex of thermophilic ATP synthase has the ability to bind ATP.
The isolated epsilon subunit of F(1)-ATPase from thermophilic Bacillus PS3 (TF(1)) binds ATP [Y. Kato-Yamada, M. Yoshida, J. Biol. Chem. 278 (2003) 36013]. The obvious question is whether the ATP binding concern with the regulation of ATP synthase activity or not. If so, the epsilon subunit even in the ATP synthase complex should have the ability to bind ATP. To check if the ATP binding to the epsilon subunit within the ATP synthase complex may occur, the gammaepsilon sub-complex of TF(1) was prepared and ATP binding was examined. The results clearly showed that the gammaepsilon sub-complex can bind ATP.
Iizuka
Satoshi
S
Department of Life Science, College of Science, Rikkyo University, 3-34-1, Nishi-Ikebukuro, Tokyo 171-8501, Japan.
Kato
Shigeyuki
S
Yoshida
Masasuke
M
Kato-Yamada
Yasuyuki
Y
D000255:Adenosine Triphosphate / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology*; D001665:Binding Sites; D004789:Enzyme Activation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D006180:Proton-Translocating ATPases / Q000737:chemistry*
D021122:Protein Subunits; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases