title: Distinct expression of Kaposi's sarcoma-associated herpesvirus-encoded proteins in Kaposi's sarcoma and multicentric Castleman's disease.
abstract: The expression of Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8)-encoded proteins is herein demonstrated in Kaposi's sarcoma (KS) and multicentric Castleman's disease (MCD) in a single lymph node derived from a patient with acquired immunodeficiency syndrome. Immunohistochemistry revealed that both lytic and latent KSHV proteins were expressed in cells of the MCD lesion. KSHV-encoded viral interleukin-6 was also detected in follicular dendritic cells of the germinal center. Cytoplasmic localization of open reading frame 59 protein and latency-associated nuclear antigen suggested KSHV activation in the MCD lesion. Moreover, a high copy number of KSHV was detected in the blood. Clinically, pegylated-liposomal doxorubicin induced regression of not only KS, but also lymphadenopathy of the MCD lesion with a decrease in KSHV load and human interleukin-6 in the blood. To the best of the authors' knowledge this is the first case demonstrating differential expression of virus proteins in two KSHV-associated diseases, KS and MCD, in the same section. The case confirms lytic KSHV infection in MCD, and suggests that clinical symptoms of MCD might be closely linked with KSHV activation.
authors.affiliation: AIDS Clinical Center, Tokyo, Japan.
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Abe
authors.forename: Daisuke
authors.initials: D
authors.lastname: Matsubara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Gatanaga
authors.forename: Shinichi
authors.initials: S
authors.lastname: Oka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kimura
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sasao
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Saitoh
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fujii
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sato
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Sata
authors.forename: Harutaka
authors.initials: H
authors.lastname: Katano
mesh_terms: D000328:Adult; D000956:Antigens, Viral / Q000235:genetics; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D005871:Castleman Disease / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics; D012514:Sarcoma, Kaposi / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000956:Antigens, Viral; D004273:DNA, Neoplasm; D004279:DNA, Viral; D015850:Interleukin-6; D009687:Nuclear Proteins; C110348:ORF59 protein, Human herpesvirus 8; D014764:Viral Proteins; C109540:latency-associated nuclear antigen