Transcription profile in mouse four-cell, morula, and blastocyst: Genes implicated in compaction and blastocoel formation.
To gain insight into early embryo development, we utilized microarray technology to compare gene expression profiles in four-cell (4C), morula (MO), and blastocyst (BL) stage embryos. Differences in spot intensities were normalized, and grouped by using Avadis Prophetic software platform (version 3.3, Strand Genomics Ltd.) and categories were based on the PANTHER and gene ontology (GO) classification system. This technique identified 622 of 7,927 genes as being more highly expressed in MO when compared to 4C (P < 0.05); similarly, we identified 654 of 9,299 genes as being more highly expressed in BL than in MO (P < 0.05). Upregulation of genes for cytoskeletal, cell adhesion, and cell junction proteins were identified in the MO as compared to the 4C stage embryos, this means they could be involved in the cell compaction necessary for the development to the MO. Genes thought to be involved in ion channels, membrane traffic, transfer/carrier proteins, and lipid metabolism were also identified as being expressed at a higher level in the BL stage embryos than in the MO. Real-time RT-PCR was performed to confirm differential expression of selected genes. The identification of the genes being expressed in here will provide insight into the complex gene regulatory networks effecting compaction and blastocoel formation.
Cui
Xiang-Shun
XS
Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea.
Li
Xing-Yu
XY
Shen
Xing-Hui
XH
Bae
Yong-Ju
YJ
Kang
Jason-Jongho
JJ
Kim
Nam-Hyung
NH
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D017931:DNA Primers; D047108:Embryonic Development / Q000235:genetics; D005784:Gene Amplification; D020869:Gene Expression Profiling; D051379:Mice; D009028:Morula / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D017931:DNA Primers; D012313:RNA