title: Soybean (Glycine max) transformation using mature cotyledonary node explants.
abstract: Agrobacterium tumefaciens-mediated transformation of soybeans has been steadily improved since its development in 1988. Soybean transformation is now possible in a range of genotypes from different maturity groups using different explants as sources of regenerable cells, various selectable marker genes and selective agents, and different A. tumefaciens strains. The cotyledonary-node method has been extensively investigated and across a number of laboratories yields on average greater than 1% transformation efficiency (one Southern-positive, independent event per 100 cotyledonary-node explants). Continued improvements in the cotyledonary-node method concomitant with further increases in transformation efficiency will enhance broader adoption of this already productive transformation method for use in crop improvement and functional genomics research efforts.
authors.affiliation: BASF Plant Service, Research Triangle Park, NC, USA.
authors.forename: Paula M
authors.initials: PM
authors.lastname: Olhoft
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Donovan
authors.forename: David A
authors.initials: DA
authors.lastname: Somers
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000254:growth & development; D018548:Cotyledon / Q000235:genetics* / Q000382:microbiology; D004351:Drug Resistance / Q000235:genetics; D018014:Gene Transfer Techniques; D005819:Genetic Markers; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology; D013025:Glycine max / Q000235:genetics* / Q000382:microbiology; D014170:Transformation, Genetic
chemical_list: D005819:Genetic Markers