title: Impact of 20:4n-6 supplementation on the fatty acid composition and hemocyte parameters of the Pacific oyster Crassostrea gigas.
abstract: Arachidonic acid (20:4n-6, ArA) and its eicosanoid metabolites have been demonstrated to be implicated in immune functions of vertebrates, fish, and insects. Thus, the aim of this study was to assess the impact of ArA supplementation on the FA composition and hemocyte parameters of oysters Crassostrea gigas. Oyster dietary conditioning consisted of direct addition of ArA solutions at a dose of 0, 0.25, or 0.41 microg ArA per mL of seawater into tanks in the presence or absence of T-Iso algae. Results showed significant incorporation of ArA into gill polar lipids when administered with algae (up to 19.7%) or without algae (up to 12.1%). ArA supplementation led to an increase in hemocyte numbers, phagocytosis, and production of reactive oxygen species by hemocytes from ArA-supplemented oysters. Moreover, the inhibitory effect of Vibrio aestuarianus extracellular products on the adhesive proprieties of hemocytes was lessened in oysters fed ArA-supplemented T-Iso. All changes in oyster hemocyte parameters reported in the present study suggest that ArA and/or eicosanoid metabolites affect oyster hemocyte functions.
authors.affiliation: UMR 100 Physiologie and Ecophysiologie des Mollusques Marins, Centre IFREMER de Brest, BP70, 29280 Plouzané, France.
authors.forename: Maryse
authors.initials: M
authors.lastname: Delaporte
authors.forename: Philippe
authors.initials: P
authors.lastname: Soudant
authors.forename: Jeanne
authors.initials: J
authors.lastname: Moal
authors.forename: Emilie
authors.initials: E
authors.lastname: Giudicelli
authors.forename: Christophe
authors.initials: C
authors.lastname: Lambert
authors.forename: Catherine
authors.initials: C
authors.lastname: Séguineau
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Samain
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry / Q000187:drug effects; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D002448:Cell Adhesion; D002470:Cell Survival; D049896:Crassostrea / Q000737:chemistry* / Q000187:drug effects*; D019587:Dietary Supplements; D056890:Eukaryota / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry*; D005880:Gills / Q000737:chemistry; D006434:Hemocytes / Q000737:chemistry / Q000502:physiology*; D010586:Phagocytes / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000097:blood / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D017382:Reactive Oxygen Species; D016718:Arachidonic Acid