Detection of H. pylori infection by ELISA and Western blot techniques and evaluation of anti CagA seropositivity in adult Turkish dyspeptic patients.
OBJECTIVE
To detect H pylori infection and to evaluate the anti CagA seropositivity in adult Turkish dyspeptic patients.


METHODS
We evaluated anti-H pylori IgA, IgG and anti-CagA antibodies using commercial enzyme-linked immunoassay (ELISA) and Western blot in dyspeptic Turkish patients. H pylori status was determined by histology and rapid urease testing.


RESULTS
Fifty-six patients were entered. Forty-eight (85.7%) out of the 56 patients were positive for H pylori. H pylori IgG seropositivity was 82.1%, IgA seropositivity 48.2%. CagA ELISA showed that IgG was positive in 50% and IgA in 30.4% of those with H pylori infections. Western blot showed that IgG seropositivity was 80.4% and IgA seropositivity 33.9%. Western blot detected IgG antibodies with reactivity to CagA in 50%, VacA in 62.5%, UreB in 87.5%, UreA in 80.4%, and OMP in 57.1%. None of the tests had a sensitivity and specificity above 80%.


CONCLUSIONS
None of these commercial tests seems clinically useful for H pylori detection in adult dyspeptic patients, while Western blot can give seropositivity and determine anti-CagA, VacA virulence factor status of Turkish dyspeptic patients in the Izmir region.
Yilmaz
Ozlem
O
Department of Micro-biology and Clinical Microbiology, Faculty of Medicine, Dokuz Eylul University, Inciralti 35340, Izmir, Turkey. ozlem.yilmaz@deu.edu.tr
Sen
Nazime
N
Küpelioğlu
Ahmet Ali
AA
Simşek
Ilkay
I
D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000097:blood* / Q000235:genetics*; D001426:Bacterial Proteins / Q000097:blood* / Q000235:genetics*; D001706:Biopsy; D015153:Blotting, Western / Q000379:methods*; D004415:Dyspepsia / Q000097:blood* / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D016481:Helicobacter Infections / Q000097:blood* / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D014421:Turkey; D037521:Virulence Factors
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; D037521:Virulence Factors; C080866:cagA protein, Helicobacter pylori