Influence of porcine genotype on the abundance of thyroid hormones and leptin in sow milk and its impact on growth, metabolism and expression of key adipose tissue genes in offspring.
Neonatal mortality is greater in commercial porcine genotypes, compared with the ancient Meishan breed that rapidly lay down adipose tissue; this may be related to hormones, such as triiodothyronine (T(3)) or leptin. Leptin is present in maternal milk; however, the extent to which this supply provides the neonate with leptin is unknown, but may play a role in growth and development. We investigated whether thyroid hormones and leptin concentrations in maternal milk differed between genotypes; and whether this influenced piglet concentrations or expression of genes involved in adipose tissue regulation. Eight Meishan and six commercial sows were entered into the study and milk samples from the day of parturition to day 4 postpartum was taken daily. The median birth weight piglet in each litter had a daily venous blood sample taken and was euthanised on day 4. Gene expressions of IGF-I, IGF-binding protein 3 (IGFBP-3), peroxisome proliferators activated receptor (PPAR)gamma and glucocorticoid receptor (GR) were measured in adipose tissue using real-time PCR. T(3) was increased in Meishan milk, but not in piglet plasma. Milk thyroxine was similar between breeds but commercial piglet levels were significantly higher. Leptin was higher in commercial sow milk throughout the study. Milk leptin was strongly correlated to plasma leptin during the first postnatal days and also to organ and body weight in Meishan piglets that also had significantly higher expression of GR, but not IGF-I, IGFBP-3 or PPARgamma. In conclusion, we have found a significant disparity in the provision of thyroid hormones in Meishan and commercial sow's milk. These changes are not always translated to plasma concentrations of hormone in the piglet. Leptin appears to have a stronger role in growth and development in the Meishan genotype compared with commercial; along with the increased GR expression, this may also represent a potential mechanism behind the rapid accumulation of adipose tissue in Meishan piglets.
Mostyn
Alison
A
Institute of Clinical Research, Centre for Reproduction and Early Life, University Hospital, Nottingham NG7 2UH, UK. alison.mostyn@nottingham.ac.uk
Sebert
Sylvain
S
Litten
Jennie C
JC
Perkins
Katharine S
KS
Laws
John
J
Symonds
Michael E
ME
Clarke
Lynne
L
D000273:Adipose Tissue / Q000254:growth & development* / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight; D005260:Female; D015870:Gene Expression; D005838:Genotype; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000235:genetics; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D020738:Leptin / Q000032:analysis* / Q000097:blood; D008892:Milk / Q000737:chemistry*; D009929:Organ Size; D047495:PPAR gamma / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D034421:Sus scrofa; D013552:Swine / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D013963:Thyroid Hormones / Q000032:analysis* / Q000097:blood; D014284:Triiodothyronine / Q000032:analysis / Q000097:blood
D018972:Insulin-Like Growth Factor Binding Protein 3; D020738:Leptin; D047495:PPAR gamma; D011965:Receptors, Glucocorticoid; D013963:Thyroid Hormones; D014284:Triiodothyronine; D007334:Insulin-Like Growth Factor I