title: Initial in vitro biological response to contemporary endodontic sealers.
abstract: The objective of this study was to evaluate the cytotoxicity of three endodontic sealers (AH Plus/Maillefer-Dentsply, Epiphany/Pentron, GuttaFlow, Coltene-Whaledent). Materials were mixed according to the manufacturer instructions and packed into Teflon molds (10 x 1 mm). For cytotoxicity testing (MTT method), the specimens were placed in contact with cultured cells, then evaluated at two subsequent time points (24 or 72 h). In addition to testing the mixed materials, 5 microl of primer liquid (GuttaFlow and Epiphany) and resin solvents (HEMA, ethanol, sterile water, or acetone) were added directly in culture for 24 and 72 h. The results showed that most materials pose significant cytotoxic risks and that cytotoxicity generally increased with time. At 72 h, GuttaFlow became significantly less toxic than AH Plus, Epiphany sealer, and Resilon. The current results support the need to continue to develop better endodontic sealers that combine the excellent sealing and bonding properties of resins with acceptable biological properties for endodontic applications.
authors.affiliation: Department of Cariology and Endodontology, University of Geneva, Geneva, Switzerland. serge.bouillaguet@medecine.unige.ch
authors.forename: Serge
authors.initials: S
authors.lastname: Bouillaguet
authors.forename: John C
authors.initials: JC
authors.lastname: Wataha
authors.forename: Franklin R
authors.initials: FR
authors.lastname: Tay
authors.forename: Martha G
authors.initials: MG
authors.lastname: Brackett
authors.forename: Petra E
authors.initials: PE
authors.lastname: Lockwood
mesh_terms: D000096:Acetone / Q000737:chemistry / Q000633:toxicity; D000818:Animals; D041702:BALB 3T3 Cells; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity*; D004396:Coloring Agents; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000633:toxicity*; D004338:Drug Combinations; D004853:Epoxy Resins / Q000737:chemistry / Q000633:toxicity; D000431:Ethanol / Q000737:chemistry / Q000633:toxicity; D006171:Gutta-Percha / Q000737:chemistry / Q000633:toxicity*; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry / Q000633:toxicity; D051379:Mice; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000633:toxicity*; D012997:Solvents / Q000737:chemistry / Q000633:toxicity; D013385:Succinate Dehydrogenase / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D014867:Water
chemical_list: D001672:Biocompatible Materials; D004396:Coloring Agents; D004129:Dimethylpolysiloxanes; D004338:Drug Combinations; D004853:Epoxy Resins; C515718:GuttaFlow; D008689:Methacrylates; D012387:Root Canal Filling Materials; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; C486893:epiphany sealer; C534916:epoxy resin-based root canal sealer; D014867:Water; D000096:Acetone; D000431:Ethanol; C005044:hydroxyethyl methacrylate; D006171:Gutta-Percha; D013385:Succinate Dehydrogenase; C022616:thiazolyl blue