title: Hybrid transcription-mediated transgene regulation in plastids.
abstract: Plastids are semi-autonomous plant organelles with their own transcription and translation machinery that are suitable for producing high levels of transgenic proteins with a bona fide structure. However, the transcription of endogenous plastid genes is largely dependent on nuclear-encoded transcription factors, and the identification of these factors has implications for transgene regulation. A novel transcription system has recently been reported that works independently of the plastid transcription machinery and is potentially useful for transgene containment and to express genes with the assurance of high-level protein accumulation.
authors.affiliation: National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Postcode 38000 Jhang Road, Faisalabad, Pakistan. sarwarkhan_40@hotmail.com
authors.forename: Muhammad Sarwar
authors.initials: MS
authors.lastname: Khan
mesh_terms: D018506:Gene Expression Regulation, Plant; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D018087:Plastids / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D050436:Regulatory Elements, Transcriptional; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D019076:Transgenes
chemical_list: D011993:Recombinant Fusion Proteins; D014157:Transcription Factors