Detection and modelling of DNA hybridization by EIS measurements. Mention of a polythiophene matrix suitable for electrochemically controlled gene delivery.
A conducting polymer sensor for direct label-free DNA detection based on a polythiophene bearing an electroactive linker group is investigated. DNA hybridization is studied by electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance (QCM) techniques. Modelling of DNA hybridization by EIS measurements exhibits the contribution of nucleic acid to a superficial p-doping process. A 675-mer single-stranded DNA is produced using asymmetric PCR from a DNA sequence of a transposable element mariner and hybridized to the previously immobilized probe. Electrochemical stimulus leads to the release "on demand" of DNA fragments and the amount delivery permits to do PCR amplification.
Gautier
Christelle
C
L.C.O.M. Chimie des Polymères (UCO2M, UMR CNRS 6011), Université du Maine, Avenue O. Messiaen, 72085 Le Mans Cedex 9, France.
Cougnon
Charles
C
Pilard
Jean-François
JF
Casse
Nathalie
N
Chénais
Benoît
B
Laulier
Marc
M
D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D018014:Gene Transfer Techniques; D009693:Nucleic Acid Hybridization; D011108:Polymers; D013057:Spectrum Analysis; D013876:Thiophenes
D011108:Polymers; D013876:Thiophenes; C066730:polythiophene; D004247:DNA