title: Impact of coccidial infection on vaccine- and vvIBDV in lymphoid tissues of SPF chickens as detected by RT-PCR.
abstract: BACKGROUND
  This study aimed at investigating a potential effect caused by coccidia on the immune response to vaccine- and very virulent infectious bursal disease virus (vvIBDV) in SPF chickens.
  
  
  METHODS
  Two groups of three weeks old SPF chickens were vaccinated prior to inoculation with coccidia and challenge with virulent IBDV, all within a period of eight days. Two control groups were similarly treated, except that challenge with field virus was omitted in one group while inoculation with coccidia was omitted in the other group. Clinical signs, lesions in the intestines caused by coccidia, lesions in the bursa of Fabricius caused by IBDV, IBDV-antibody titres, and virus detection by reverse transcription polymerase chain reaction (RT-PCR) were compared among the groups. Lymphoid tissues and swab samples were analysed by general RT-PCR, and positive results were identified by strain specific duplex (DPX) RT-PCR.
  
  
  RESULTS
  In the triple-infected groups, vaccine strain IBDV was detected in spleen and thymus tissues, and no field virus was detected in bursa samples, contrary to the double-infected groups.
  
  
  CONCLUSIONS
  The results suggest an enhancing effect on the immune response caused by subclinical coccidiosis and vvIBDV acting in concert.
authors.affiliation: Danish Institute for Food and Veterinary Research, Hangøvej 2, DK-8200 Aarhus N, Denmark. ska@dfvf.dk
authors.forename: Susanne
authors.initials: S
authors.lastname: Kabell
authors.forename: Kurt J
authors.initials: KJ
authors.lastname: Handberg
authors.forename: Magne
authors.initials: M
authors.lastname: Bisgaard
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000150:complications / Q000276:immunology*; D002060:Bursa of Fabricius / Q000473:pathology; D003048:Coccidiosis / Q000150:complications / Q000276:immunology / Q000662:veterinary*; D016786:Eimeria tenella / Q000472:pathogenicity*; D007243:Infectious bursal disease virus / Q000276:immunology* / Q000472:pathogenicity; D007422:Intestines / Q000473:pathology; D008221:Lymphoid Tissue / Q000821:virology; D011200:Poultry; D011201:Poultry Diseases / Q000276:immunology*; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D014774:Virulence
chemical_list: D012367:RNA, Viral