Rapid analytical detection of microcystins using gold colloidal immunochromatographic strip.
Routine monitoring of microcystin in natural waters is difficult because the concentration of the toxin is low and the detection method is usually complicated. We developed a rapid analytical detection method of microcystins gold colloidal immunochromatogeraphic strip. The sensitivity of the strip is about 1 ng/mL for microcystin LR; it is able to distinguish visually among different concentrations of microcystin solutions. The developed gold colloidal strip can detect microcystins within 15 min and does not require either a complicated extraction system, or trained or qualified experts.
Pyo
Dongjin
D
Department of Chemistry, Kangwon National University, Chunchon 200-701, South Korea.
Choi
Jongchon
J
Hong
Jonguk
J
Oo
Hlaing Hlaing
HH
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis; D000937:Antigen-Antibody Reactions; D001427:Bacterial Toxins / Q000032:analysis*; D002845:Chromatography / Q000379:methods; D003102:Colloids / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006046:Gold / Q000737:chemistry*; D052998:Microcystins; D046931:Microcystis / Q000737:chemistry; D008968:Molecular Conformation; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000032:analysis*; D011934:Reagent Strips; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013997:Time Factors; D014873:Water Pollutants / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001427:Bacterial Toxins; D003102:Colloids; D052998:Microcystins; D010456:Peptides, Cyclic; D011934:Reagent Strips; D014873:Water Pollutants; D006046:Gold; C078588:microcystin