title: Demonstration of G . U wobble base pairs by Raman and IR spectroscopy.
abstract: When guanine and uracil form hydrogen bonds in the pairing scheme first proposed by Crick one would expect that poly(A,G) will form an unperturbed double helix with poly U at room temperature in a dilute electrolyte solution (0.1 M NaCl). We have demonstrated by Raman- and IR-spectroscopy that the secondary structure of poly(A.G) . poly U is very similar to the structure of poly A . poly U; only the thermal stability of the double helix seems slightly lower than the stability of poly A . poly U, whereas the average helix length is unaffected by the dispersed G . U base pairs. From our input ratio of guanine and adenine we estimate that about every fourth base pair is a wobble pair.
authors.affiliation: Inst. Physik. Chemie, Universität Freiburg, W. Germany.
authors.forename: T
authors.initials: T
authors.lastname: Ackermann
authors.forename: V
authors.initials: V
authors.lastname: Gramlich
authors.forename: H
authors.initials: H
authors.lastname: Klump
authors.forename: T
authors.initials: T
authors.lastname: Knäble
authors.forename: E D
authors.initials: ED
authors.lastname: Schmid
authors.forename: H
authors.initials: H
authors.lastname: Seliger
authors.forename: J
authors.initials: J
authors.lastname: Stulz