[Example of examination of allergic substances (egg, milk) in commercial foods].
Allergic substances (eggs, milk) in commercial foods were measured by means of the notified ELISA methods using FASTKIT ELISA kit (N kit) and "Tokuteigenzairyo sokutei kit" (M kit). Some samples were also analyzed with the notified western blot method using "Tokuteigenzairyo western blot kit". In the methods for detection of eggs, proteins of fish egg and chicken muscle were examined using the ELISA and Western blotting methods to check for cross-reaction. Sujiko was false positive with the M kit. Difficulties were also encountered in the notified ELISA and Western blotting methods with raw chicken. In foods labeled as containing eggs, it was difficult to detected in the heart-processed food using both notified methods. In the method for detection of milk, foods containing thickening polysaccharides, showed cross-reaction with the N kit. However, this cross-reaction was eliminated when an improved N kit was used. Foods labeled as containing milk did not present any difficulty.
Shimoi
Toshiko
T
Tokyo Metropolitan Institute of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
Ushiyama
Hirofumi
H
Kan
Kimiko
K
Saito
Kazuo
K
Kamata
Kunihiro
K
D000485:Allergens / Q000032:analysis*; D015153:Blotting, Western / Q000379:methods; D004531:Eggs / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005189:False Positive Reactions; D005504:Food Analysis / Q000379:methods*; D007202:Indicators and Reagents; D008894:Milk Proteins / Q000032:analysis*
D000485:Allergens; D007202:Indicators and Reagents; D008894:Milk Proteins