Quantification and identification of polyomavirus DNA in blood and urine of renal transplant recipients.
A cohort of 201 kidney transplant recipients (KTR) including 7 patients with evidence of renal function deterioration (as defined by creatinine levels >20% over baseline values) was analyzed for polyomavirus DNA in blood and urine samples by a new quantitative polymerase chain reaction method. Of 201 patients, 14 (6.9%) were positive for polyomavirus DNA in blood (median level, 500 copies per milliliter of blood) including all 7 patients with renal function deterioration. Polyomavirus DNA detection in blood for diagnosis of renal function deterioration in KTR showed a sensitivity of 100% and a specificity of 96%, whereas positive and negative predictive values were 50% and 100%, respectively. Diagnostic value of decoy cells detection and polyomavirus DNA quantification in urine samples was negligible.
Baldanti
Fausto
F
Servizio di Virologia, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy.
Fogazzi
Gian Battista
GB
Furione
Milena
M
Saglimbeni
Lucia
L
Rovida
Francesca
F
Gatti
Marta
M
Paolucci
Stefania
S
Tarantino
Antonio
A
Gerna
Giuseppe
G
D000293:Adolescent; D000328:Adult; D000368:Aged; D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D015331:Cohort Studies; D004279:DNA, Viral / Q000097:blood* / Q000652:urine; D005260:Female; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification*; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D027601:Polyomavirus Infections / Q000097:blood* / Q000235:genetics* / Q000652:urine; D011237:Predictive Value of Tests
D004279:DNA, Viral