title: Genetic variants of beta-lactoglobulin gene and its association with milk composition traits in riverine buffalo.
abstract: A study was carried out to determine genetic variants of beta-lactoglobulin gene and to explore associations between these and milk composition traits in riverine buffalo. Single strand conformation polymorphism was employed to detect the genetic variants of the gene. Two fragments of this gene i.e. 119 bp of exon I and 400 bp spanning exon IV and intron IV were included in the study. For 119 bp fragment, three alleles namely, A, B and C were observed in all the buffalo breeds whereas four alleles (A, B, C and D) were detected for 400 bp fragment. The frequency distribution of alleles was different in different breeds of buffaloes for both the fragments. For exon I fragment, the milk composition traits such as total SNF, protein, solid, fat and whey protein yield were found to be significantly (P<0.05) associated with genotypes in Murrah and Bhadawari buffalo whereas in Mehsana breed genotypes were significantly (P<0.05) co-related with total SNF, solid and fat yield. Genotypes of 400 bp fragment, only total fat yield in Mehsana buffalo was found to be significantly (P<0.05) associated with genotypes.
authors.affiliation: Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P.-243 122, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Vohra
authors.forename: Tarun
authors.initials: T
authors.lastname: Kumar Bhattacharya
authors.forename: Shanker
authors.initials: S
authors.lastname: Dayal
authors.forename: Pushpendra
authors.initials: P
authors.lastname: Kumar
authors.forename: Arjava
authors.initials: A
authors.lastname: Sharma
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes; D005091:Exons; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D007438:Introns; D007782:Lactoglobulins / Q000235:genetics*; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
chemical_list: D007782:Lactoglobulins