Expression of aromatase and 5-alpha-reductase genes in endometrial adenocarcinoma.
The aim of this study was to investigate the expression of genes coding aromatase and 5-alpha-reductase type 1 and type 2 in endometrial adenocarcinoma, with parallel analysis of testosterone concentrations in serum isolated from blood of basilic and ovarian vein. A significant difference between the copy numbers of mRNA for 5-alpha-reductase type 1 and type 2 was disclosed. The content of the mRNA copies was higher for 5-alpha-reductase type 2 than for type 1. We noted a positive correlation between the mRNA copy number for aromatase and 5-alpha-reductase type 1 as well as between the mRNA copy number for aromatase and 5-alpha-reductase type 2. A positive correlation was disclosed between the mRNA copy number for 5-alpha-reductase type 1 and type 2. We concluded that in tissues of endometrial adenocarcinoma, there is expression of aromatase and 5-alpha-reductase type 1 and 2 genes, and that the expression of aromatase and 5-alpha-reductase genes in tissues of endometrial adenocarcinoma is not controlled by testosterone.
Kazmierczak
Wojciech
W
Department of Perinatology and Gynecology, Medical University of Silesia, Plac Traugutta 6, 41-800 Zabrze, Poland.
Skalba
Piotr
P
Dabkowska-Huc
Anna
A
Samojedny
Arkadiusz
A
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000235:genetics*; D000230:Adenocarcinoma / Q000097:blood* / Q000235:genetics*; D001141:Aromatase / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000097:blood* / Q000235:genetics*; D005260:Female; D018628:Gene Dosage; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D012333:RNA, Messenger; D013739:Testosterone / Q000097:blood
D007527:Isoenzymes; D012333:RNA, Messenger; D013739:Testosterone; D001141:Aromatase; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase