Modifying professional antigen-presenting cells to enhance DNA vaccine potency.
DNA vaccines have emerged as a potentially important form of vaccination in the control of infectious diseases and cancers. It is now clear that professional antigen-presenting cells (APCs), such as dendritic cells (DCs) play important roles in generating humoral and cell-mediated antigen-specific immune responses by DNA vaccination. Continuing progress in our understanding of how professional APCs uptake DNA, process and present the antigens encoded by DNA vaccines to activate B- and T-cell-mediated immune responses in vaccinated individuals provides a framework from which to design more effective DNA vaccines. Advances in molecular biology technology allow DNA to be easily manipulated and make the implementation of novel DNA vaccine strategies possible. This review discusses strategies employing molecular biology technology to improve DNA vaccine potency. These approaches include strategies to increase the numbers of antigen-expressing DCs, strategies to enhance MHC class I and/or II presentation of the encoded antigen and strategies to prolong the life of antigen-expressing DCs to enhance DNA vaccine potency. We will also discuss the methodology involved in DNA vaccine development targeting human papillomavirus oncogenic protein E7.
Hung
Chien-Fu
CF
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD, USA.
Yang
Mu
M
Wu
T C
TC
D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D052162:Human papillomavirus 16 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology*; D030361:Papillomavirus Infections / Q000276:immunology* / Q000517:prevention & control; D013997:Time Factors; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D009856:Oncogene Proteins, Viral; D019444:Vaccines, DNA; D014765:Viral Vaccines; C062495:oncogene protein E7, Human papillomavirus type 6