[Structures of leucogen and its related substances in solution state].
OBJECTIVE
To study the four diastereomers of leucogen and structure of the related substances.


METHODS
LC-DAD, LC-MS/MS and LC- 1H NMR were used. LC was carried out with a Phenomnex Luna C18 (250 mm x 4.60 mm ID, 5 microm) column and a mobile phase of water-acetonitrile-glacial acetic acid (58:42: 0.3).


RESULTS
The structures of leucogen and its related substances were identified. Leucogen and the related substances were found to have four diastereomers in solution state separately. The stability and transformation of the four diastereomers were analyzed and 3R4S5R was found to be more stable than the others according to quantum calculations.


CONCLUSIONS
Leucogen have four diastereomers in solution state and it can transform from one diastereomer to the others, and the 3R4S5R is more stable than the others.
Zheng
Xiu-wen
XW
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.
Qian
Wen
W
Hang
Tai-jun
TJ
Ma
Hai-bo
HB
Zhang
Zheng-xing
ZX
D002853:Chromatography, Liquid; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011789:Quantum Theory; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization; D013237:Stereoisomerism; D053778:Thiazolidines / Q000032:analysis / Q000737:chemistry*
D012996:Solutions; D053778:Thiazolidines; C001176:Leyk