Cerebrospinal fluid of Alzheimer's disease and dementia with Lewy bodies patients enhances alpha-synuclein fibril formation in vitro.
Deposition of alpha-synuclein (alphaS) aggregates inside brain cells is a pathological hallmark of several neurodegenerative diseases, including Parkinson's disease (PD), and dementia with Lewy bodies (DLB). Recently, extracellular alphaS was detected in cerebrospinal fluid (CSF) and plasma of humans. We investigated whether CSF influences alphaS aggregation in vitro using fluorescence spectroscopy with thioflavin S and electron microscopy. We found that CSF obtained from Alzheimer's disease (AD) and DLB patients enhanced the alphaS fibril formation compared with tauopathy and non-central nervous system disease. Thus, CSF of AD and DLB characterized by aggregation of Abeta or alphaS might promote falphaS formation.
Ono
Kenjiro
K
Department of Neurology and Neurobiology of Aging, Kanazawa University Graduate School of Medical Science, Kanazawa 920-8640, Japan.
Noguchi-Shinohara
Moeko
M
Yoshita
Mitsuhiro
M
Naiki
Hironobu
H
Yamada
Masahito
M
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid*; D016229:Amyloid beta-Peptides / Q000378:metabolism; D001057:Apolipoproteins E / Q000134:cerebrospinal fluid; D052160:Benzothiazoles; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D020961:Lewy Body Disease / Q000134:cerebrospinal fluid*; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D011569:Psychiatric Status Rating Scales; D013050:Spectrometry, Fluorescence; D013844:Thiazoles / Q000378:metabolism; D051844:alpha-Synuclein / Q000502:physiology*; D016875:tau Proteins / Q000378:metabolism
D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E; D052160:Benzothiazoles; D007074:Immunoglobulin G; D013844:Thiazoles; D051844:alpha-Synuclein; D016875:tau Proteins; C009462:thioflavin T