Activity of matrix metalloproteinase-2 (MMP-2) in canine oronasal tumors.
Activity of matrix metalloprotease-2 (MMP-2) and the expression of its related molecules were examined in spontaneous canine oronasal tumors. Tissue samples from melanoma and squamous cell carcinoma possessed higher MMP-2 activity, as shown in gelatin zymography, in comparison with acanthomatous epulis and nasal adenocarcinoma. Regional lymph node invasion and distant metastases were more frequently observed in the MMP-2 positive cases. There were no significant differences by RT-PCR examination in the expression of the genes encoding MMP-2, MT1-MMP and TIMP-2 among the tumor histological types. However, the MMP-2/TIMP-2 ratio showed a significantly higher level of the genes in the malignant oral melanoma and squamous cell carcinoma. The MMP-2/TIMP-2 ratio was also positively correlated with MMP-2 activity in gelatin zymography. These results indicate that the MMP-2/TIMP-2 ratio may be of value in evaluating the prognosis in canine oronasal cavity tumors.
Nakaichi
Munekazu
M
Department of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University, 1677-1 Ooaza Yoshida, Yamaguchi 753-8515, Japan. nakaichi@yamaguchi-u.ac.jp
Yunuki
Toshi
T
Okuda
Masaru
M
Une
Satoshi
S
Taura
Yasuho
Y
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000201:enzymology* / Q000473:pathology; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D008207:Lymphatic Metastasis; D008297:Male; D053511:Matrix Metalloproteinase 14 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008545:Melanoma / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D009062:Mouth Neoplasms / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D009669:Nose Neoplasms / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D018709:Statistics, Nonparametric; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D053511:Matrix Metalloproteinase 14