title: [Evaluation of sperm mitochondrial function using Rh123/PI dual fluorescent staining].
abstract: OBJECTIVE
  To investigate the feasibility and clinical significance of detecting sperm mitochondrial function by using Rh123/PI dual fluorescent staining and flow cytometry analysis, and to explore the relationship between the results of Rh123/PI dual fluorescent staining and seminal parameters.
  
  
  METHODS
  Sixty-three semen samples were classified as normal (n=31) and abnormal (n=32) according to the World Health Organization guidelines. Rh123/PI dual fluorescent staining was then carried out to evaluate sperm mitochondrial function by flow cytometry analysis.
  
  
  RESULTS
  Significant differences in Rh123+ PI-, Rh123- /PI+ and Rh123- /PI- were detected between the normal and abnormal semen samples (P < 0.05). There was a significant positive correlation between the Rh123+ PI- sperm and sperm motility and a significant inverse correlation between Rh123+ PI- and immotile sperm. But the Rh123- PI+ sperm showed a contrary relationship with Rh123+ PI-. A significant inverse correlation was also observed between the Rhl23- /PI- sperm and sperm concentration in the abnormal group.
  
  
  CONCLUSIONS
  Rh123/PI dual fluorescent staining and flow cytometry analysis can readily and quickly detect sperm mitochondrial function and be used to evaluate semen quality.
authors.affiliation: Laboratory of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, Nanjing, Jiangsu 210002, China. wuyongming8088@163.com
authors.forename: Yong-ming
authors.initials: YM
authors.lastname: Wu
authors.forename: Xin-yi
authors.initials: XY
authors.lastname: Xia
authors.forename: Lian-jun
authors.initials: LJ
authors.lastname: Pan
authors.forename: Yong
authors.initials: Y
authors.lastname: Shao
authors.forename: Bao-fang
authors.initials: BF
authors.lastname: Jin
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Huang
authors.forename: Xiu-lai
authors.initials: XL
authors.lastname: Wang
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000502:physiology*; D020112:Rhodamine 123; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D020112:Rhodamine 123