Antioxidant and cancer cell proliferation inhibition effect of citrus pectin-oligosaccharide prepared by irradiation.
Pectin was dissolved in deionized distilled water (2%, vol/vol) and irradiated at 20 kGy using a Co-60 gamma ray irradiator. The resulting solution was dialyzed and lyophilized. The samples were separated into three groups to estimate their antioxidant and cancer cell proliferation effects: non-irradiated (0 kGy), irradiated (20 kGy), and dialyzed (20 kGy-F, mol wt <10,000) samples. Antioxidant properties of each treatment was tested by a beta-carotene-linoleic acid bleaching assay and electron donating ability and compared for antioxidant index, which indicated that the activity was higher in the order of 20 kGy-F > 20 kGy > 0 kGy. Spleen cell survival effect of the irradiated pectin (20 kGy) and dialyzed (20 kGy-F) samples was higher than the non-irradiated control (0 kGy). The pectins inhibited growth of the cancer cell in the order of 20 kGy- F > 20 kGy > 0 kGy. The Ames test revealed that none of the fractions was mutagenic, and there was no indication of a dose-dependent response for any of the samples. These results suggest that a functional pectin oligosaccharide can be produced by irradiation for the food industry without any chemical treatment.
Kang
Ho Jin
HJ
Department of Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, KAERI, Jeongeup, Republic of Korea.
Jo
Cheorun
C
Kwon
Joong Ho
JH
Son
Jun Ho
JH
An
Bong Jeon
BJ
Byun
Myung Woo
MW
D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D002957:Citrus / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D005720:Gamma Rays; D006801:Humans; D019787:Linoleic Acid / Q000737:chemistry / Q000494:pharmacology; D009152:Mutagenicity Tests; D009369:Neoplasms / Q000473:pathology*; D009844:Oligosaccharides / Q000494:pharmacology / Q000528:radiation effects; D010084:Oxidation-Reduction; D010368:Pectins / Q000494:pharmacology* / Q000528:radiation effects*; D010851:Picrates; D019207:beta Carotene / Q000737:chemistry / Q000494:pharmacology
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009844:Oligosaccharides; D010851:Picrates; D019207:beta Carotene; D010368:Pectins; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl