A water extract of Artemisia capillaris prevents 2,2'-azobis(2-amidinopropane) dihydrochloride-induced liver damage in rats.
A water extract of Artemisia capillaris Thunberg (Compositae) was investigated for protective effects against oxidative stress induced by 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) in Sprague-Dawley male rats. Rats were orally administered A. capillaris water extract (ACWE; 7.5 g/kg) for 7 days before AAPH treatment (60 mg/kg). AAPH intoxication significantly elevated enzyme markers of liver injury (glutamic oxaloacetic transaminase and glutamic pyruvic transaminase). The pre-administration of ACWE significantly reduced the liver-damaging effects of AAPH as indicated by the low levels of these enzymes. Moreover, the ACWE administration significantly attenuated the accumulation of thiobarbituric acid-reactive substances in both plasma and liver tissues compared with those of rats administered AAPH alone. Furthermore, ACWE administration slightly improved the liver reduced glutathione levels and enhanced the production of antioxidant enzymes like catalase. A. capillaris contained 10.1 mg of catechin in 100 g of dried sample; the high-performance liquid chromatography results showed catechin composition in the ACWE to be 28% (-)-epigallocatechin gallate, 49% (-)- epigallocatechin, and 23% other catechins. These observations clearly indicate that ACWE contains antioxidant catechins capable of ameliorating the AAPH-induced hepatic injury by virtue of its antioxidant activity.
Han
Kyu-Ho
KH
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, Republic of Korea.
Jeon
You-Jin
YJ
Athukorala
Yasantha
Y
Choi
Kang-Duk
KD
Kim
Cheon-Jei
CJ
Cho
Jin-Kook
JK
Sekikawa
Mitsuo
M
Fukushima
Michiro
M
Lee
Chi-Ho
CH
D000410:Alanine Transaminase / Q000097:blood; D000578:Amidines / Q000633:toxicity*; D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D001219:Aspartate Aminotransferases / Q000097:blood; D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis; D056486:Chemical and Drug Induced Liver Injury; D002851:Chromatography, High Pressure Liquid; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D008099:Liver / Q000737:chemistry; D008107:Liver Diseases / Q000517:prevention & control*; D008297:Male; D016877:Oxidants; D018384:Oxidative Stress; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014867:Water
D000578:Amidines; D016877:Oxidants; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D014867:Water; C046728:2,2'-azobis(2-amidinopropane); D002392:Catechin; C045651:epigallocatechin gallate; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; C057580:gallocatechol