Resistance mechanisms in an in vitro-selected amoxicillin-resistant strain of Helicobacter pylori.
We investigated the beta-lactam resistance mechanism(s) of an in vitro-selected amoxicillin-resistant Helicobacter pylori strain (AmoxR). Our results demonstrated that resistance is due to a combination of amino acid substitutions in penicillin binding protein 1 (PBP1), HopB, and HopC identified in AmoxR, resulting in decreased affinity of PBP1 for amoxicillin and decreased accumulation of penicillin.
Co
Edgie-Mark A
EM
Biomedical Sciences Division, University of California, Riverside, Riverside, CA 92521-0121, USA.
Schiller
Neal L
NL
D019943:Amino Acid Substitution / Q000235:genetics; D000658:Amoxicillin / Q000494:pharmacology*; D005798:Genes, Bacterial; D016480:Helicobacter pylori / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D046915:Penicillin-Binding Proteins / Q000235:genetics; D010406:Penicillins / Q000096:biosynthesis; D012641:Selection, Genetic; D014169:Transformation, Bacterial; D018440:beta-Lactam Resistance / Q000502:physiology
D046915:Penicillin-Binding Proteins; D010406:Penicillins; D000658:Amoxicillin