[Strategies for antibody immobilization during the fabrication of antibody microarrays].
As one of the proteomic technologies, antibody microarrays can provide a powerful tool for the high-throughput parallel analysis of thousands of proteins in a single experiment. Because antibodies are chemically and structurally complex; immobilizing antibody on solid phase surface of substrates has been a key step during the development of antibody microarrays. This review will focus on the current strategies for antibody immobilization and discuss the advantages and disadvantages of these methods.
Zhang
Lu
L
Institute of Nephrology, Zhongda Hospital, Southeast University, Nanjing 210009, China.
Liu
Bicheng
B
D000327:Adsorption; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding
D000911:Antibodies, Monoclonal