Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in sol-gel-derived ceramic-carbon nanotube nanocomposite film.
The sol-gel-derived ceramic-carbon nanotube (SGCCN) nanocomposite film fabricated by doping multiwall carbon nanotubes (MWNTs) into a silicate gel matrix was used to immobilize protein. The SGCCN film can provide a favorable microenvironment for horseradish peroxidase (HRP) to perform direct electron transfer (DET) at glassy carbon electrode. The HRP immobilized in the SGCCN film shows a pair of well-defined redox waves and retains its bioelectrocatalytic activity to the reduction of O2 and H2O2, which is superior to that immobilized in silica sol-gel film.
Chen
Hongjun
H
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Dong
Shaojun
S
D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D002516:Ceramics; D004563:Electrochemistry / Q000379:methods*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005782:Gels; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis*; D053761:Nanocomposites; D037742:Nanotubes, Carbon
D004800:Enzymes, Immobilized; D005782:Gels; D037742:Nanotubes, Carbon; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase