Effects of an enamel matrix derivative on human osteoblasts and PDL cells grown in organoid cultures.
OBJECTIVE
The objective of this study was to investigate cellular effects of enamel matrix derivative (EMD) in human derived, primary osteoblasts and periodontal ligament (PDL) cells grown in organoid cultures.


METHODS
Cell replication was assessed by BrdU-incorporation. [(3)H]-proline incorporation was measured to determine the synthesis of proline-containing proteins, such as collagen. In addition, calcium accumulation and alkaline-phosphatase-activity were quantified. Electron microscopy for morphological analysis was performed.


RESULTS
Our results showed that EMD enhances BrdU-incorporation in PDL cells and osteoblasts. Also, in osteoblast organoid cultures [3H]-proline incorporation was 3-fold increased (P < .01). Extensive matrix deposition was noted in osteoblast cultures by electron microscopy. In osteoblasts, high levels of calcium accumulation and alkaline-phosphatase-activity were found. However, EMD did not promote mineralization.


CONCLUSIONS
Our results indicate that under organoid culture conditions EMD is able to promote the synthesis of proline-containing proteins such as collagen but not matrix mineralization of primary human osteoblastic cells.
Pischon
Nicole
N
Department of Periodontology, School of Dental Medicine, Medical Faculty Charité, Humboldt-University, Berlin, Germany. npischon@charite.de
Zimmermann
Bernd
B
Bernimoulin
Jean-Pierre
JP
Hägewald
Stefan
S
D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D006801:Humans; D007223:Infant; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D055232:Proline-Rich Protein Domains; D014074:Tooth Calcification / Q000187:drug effects
D003746:Dental Enamel Proteins; D010455:Peptides; C109069:enamel matrix proteins; D000469:Alkaline Phosphatase; D001973:Bromodeoxyuridine