Imaging diacylglycerol dynamics at organelle membranes.
Fluorescence imaging is a powerful technique to visualize spatiotemporal dynamics of biomolecules in living cells. We describe fluorescent indicators for a lipid second messenger, diacylglycerol (DAG), which allow the localized analysis of DAG dynamics at subcellular membranes. We have thus pinpointed that DAG concentrations increase and/or decrease at not only the plasma membrane but also organelle membranes such as endomembranes and mitochondrial outer membranes.
Sato
Moritoshi
M
Department of Chemistry, School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Ueda
Yoshibumi
Y
Umezawa
Yoshio
Y
D000818:Animals; D002460:Cell Line; D004075:Diglycerides / Q000737:chemistry*; D004285:Dogs; D005456:Fluorescent Dyes / Q000737:chemistry*; D007425:Intracellular Membranes / Q000502:physiology*; D008856:Microscopy, Fluorescence / Q000379:methods; D051336:Mitochondrial Membranes / Q000502:physiology*
D004075:Diglycerides; D005456:Fluorescent Dyes