Induction of G2 arrest and apoptosis of raji cells by continuous low-dose beta irradiation with 188Re-perrhenate.
Continuous irradiation with exponentially reducing beta-rays induces cell death, known as apoptosis. The aim of this study was to investigate the G2 arrest and apoptosis caused by the beta-ray emitted by the radioisotope (188)Re. Doses of 0.4 Gy (3.7 MBq), 4 Gy (37 MBq), and 40 Gy (370 MBq), were added to Blymphoma Raji cells, and cell viability, apoptosis, and DNA cell-cycle changes were assayed. (188)Re showed time- and dose-dependent effects on cell viability and on cell apoptosis and necrosis. At a (188)Re dose of 0.4 Gy, G(2) cell-cycle arrest was observed after 16 hours, and 4,6-diamidino-2-phenylindole (DAPI) staining indicated a slow, time-dependent increase in apoptotic bodies. At a (188)Re dose of 40 Gy, DNA fragmentation was observed at 2 hours, indicative of early damage in the nucleus. In summary, our results showed that continuous irradiation with low-dose beta-rays induced G(2) arrest and progressive apoptosis, which may be characteristic mechanisms of radionuclide therapy.
Lim
Soo Jeong
SJ
Laboratory of Cyclotron Application, Korea Institute of Radiological and Medical Science, Seoul, Korea.
Kim
Eun Hee
EH
Woo
Kwang Sun
KS
Chung
Wee Sup
WS
Choi
Chang Woon
CW
Lim
Sang Moo
SM
D017209:Apoptosis / Q000528:radiation effects*; D001610:Beta Particles / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D016195:G2 Phase / Q000528:radiation effects*; D006801:Humans; D007211:Indoles / Q000737:chemistry; D016393:Lymphoma, B-Cell / Q000473:pathology / Q000532:radiotherapy*; D011868:Radioisotopes / Q000494:pharmacology*; D019275:Radiopharmaceuticals / Q000494:pharmacology*; D012211:Rhenium / Q000494:pharmacology*; D013194:Staining and Labeling
D007211:Indoles; D011868:Radioisotopes; D019275:Radiopharmaceuticals; C007293:DAPI; D012211:Rhenium