title: Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH.
abstract: Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide.
authors.affiliation: Department of Marine Biology, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China.
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Zhong
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhang
authors.forename: Jixiang
authors.initials: J
authors.lastname: Chen
authors.forename: Zhenghao
authors.initials: Z
authors.lastname: Chi
authors.forename: Boguang
authors.initials: B
authors.lastname: Sun
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Brian
authors.initials: B
authors.lastname: Austin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005432:Flounder / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D008969:Molecular Sequence Data; D010740:Phospholipases / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D013696:Temperature; D014733:Vibrio / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; C073845:hemolysin, Vibrio; D010740:Phospholipases