title: [Construction of multifunctonal nano-delivery system crossing blood brain barrier].
abstract: OBJECTIVE
  To construct multifunctonal nano-delivery system crossing the blood brain barrier (BBB).
  
  
  METHODS
  The magnetic nanoparticles were preprared with O-carboxylmethylated chitosan (O-CMC) and conjugated with a peptide sequence from the human immunodeficiency virus 1-tat protein and transferrin (Tf), and anti-tumor drug methotrexate (MTX), and thus constructed a O-CMC magnetic nanoparticles carrier system conjugating with Tat and Tf (O-MNPs-Tat-Tf) that combines multiple functions including crossing BBB, magnetism, receptor-mediated dual targets and anti-tumor capabilities. The appearance, diameter, and magnetism of MTX-O-MNPs-Tat-Tf carrier system were characterized with transmission electronic microscopy, atomic force microscopy and vibrating samples magnetometer. The cytotoxicity of MTX-loaded O-MNPs-Tat-Tf was investigated with C6 glioma cells. The ability of O-MNPs-Tat-Tf crossing BBB was investigated in rats by single photon emission computed tomography.
  
  
  RESULTS
  The mean particle diameter was 75 nm, along with good anti-tumor property. The multi-functioned carrier system successfully crossed the BBB in rat.
  
  
  CONCLUSIONS
  The establishment of MTX-O-MNPs-Tat-Tf carrier model implies a promising future for its application in therapy of cerebral diseases.
authors.affiliation: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yao
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Kang
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Pu
authors.forename: Jin
authors.initials: J
authors.lastname: Chang
mesh_terms: D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism; D048271:Chitosan / Q000031:analogs & derivatives / Q000737:chemistry; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006801:Humans; D008280:Magnetics; D053758:Nanoparticles; D014168:Transferrin
chemical_list: D004337:Drug Carriers; C504164:O-carboxymethylchitosan; D014168:Transferrin; D048271:Chitosan