title: Morphological analysis of three wound-cleaning processes on potentially contamined wounds in rats.
abstract: OBJECTIVE
  To evaluate the inflammatory response of potentially infected wounds treated with isotonic saline solution, chlorhexidine and PVP-I, seven days after surgery.
  
  
  METHODS
  Thirty-two male rats were used, divided into 4 groups. All animals had their surgical wounds infected with a standard bacterial inoculum. Control group (A): animals had their surgical wounds sutured without any kind of cleaning. Saline solution group (B): animals had their wounds cleaned with saline solution. Chlorhexidine group (C): animals had their wounds cleaned with chlorhexidine. PVP-I group (D): animals had their wounds cleaned with PVP-I. Seven days after surgery, all the animals had their skin submitted to microscopic and macroscopic evaluation.
  
  
  RESULTS
  Edema was found on all histological slices analyzed, as well as vascular proliferation and congestion. Groups A and D showed presence of mild neutrophilic infiltrate, and moderate lymphocytic and macrophage infiltrate. Group B showed severe neutrophilic, macrophage, and lymphocytic infiltrate. Group C showed moderate neutrophilic, macrophage, and lymphocytic infiltrate.
  
  
  CONCLUSIONS
  Group D was the group which showed inflammatory infiltrate most similar to the group that was not submitted to treatment.
authors.affiliation: Surgical Technique and Experimental Surgery, Department of Surgery, University of Santa Catarina (UFSC), SC, Brazil. dacampora@gmail.com
authors.forename: Armando José
authors.initials: AJ
authors.lastname: d'Acampora
authors.forename: Daniella Serafin Couto
authors.initials: DS
authors.lastname: Vieira
authors.forename: Marcos Túlio
authors.initials: MT
authors.lastname: Silva
authors.forename: Débora Cadore de
authors.initials: DC
authors.lastname: Farias
authors.forename: Ricardo
authors.initials: R
authors.lastname: Tramonte
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D002710:Chlorhexidine / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005355:Fibrosis / Q000473:pathology; D006097:Granulation Tissue / Q000473:pathology; D007552:Isotonic Solutions / Q000627:therapeutic use; D008297:Male; D011206:Povidone-Iodine / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000627:therapeutic use*; D013530:Surgical Wound Infection / Q000188:drug therapy* / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D000891:Anti-Infective Agents, Local; D007552:Isotonic Solutions; D012965:Sodium Chloride; D011206:Povidone-Iodine; D002710:Chlorhexidine