title: Cardenolide glycosides from Pergularia tomentosa and their proapoptotic activity in Kaposi's sarcoma cells.
abstract: Continuing our investigations on plants belonging to the Asclepiadaceae family, three new cardenolide glycosides, 3'-O-beta-D-glucopyranosylcalactin (1), 12-dehydroxyghalakinoside (2), and 6'-dehydroxyghalakinoside (3), along with the known ghalakinoside (4) and calactin (5), were isolated from the roots of Pergularia tomentosa. The structures of these compounds were elucidated by extensive spectroscopic methods including 1D- and 2D-NMR experiments as well as ESIMS analysis. The isolated cardenolides caused apoptotic cell death of Kaposi's sarcoma cells.
authors.affiliation: Faculty of Science, South Valley University, Aswan 81528, Egypt.
authors.forename: Arafa I
authors.initials: AI
authors.lastname: Hamed
authors.forename: Alberto
authors.initials: A
authors.lastname: Plaza
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Balestrieri
authors.forename: Usama A
authors.initials: UA
authors.lastname: Mahalel
authors.forename: Irina V
authors.initials: IV
authors.lastname: Springuel
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Oleszek
authors.forename: Cosimo
authors.initials: C
authors.lastname: Pizza
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D029062:Apocynaceae / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects; D002298:Cardenolides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004534:Egypt; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012514:Sarcoma, Kaposi; D021241:Spectrometry, Mass, Electrospray Ionization; D014407:Tumor Cells, Cultured
chemical_list: C514397:12-dehydroxyghalakinoside; C514396:3'-O-beta-D-glucopyranosylcalactin; C514398:6'-dehydroxyghalakinoside; D000972:Antineoplastic Agents, Phytogenic; D002298:Cardenolides; D006027:Glycosides