title: Proteomic identification of palmitoylated proteins.
abstract: A proteomic method that purifies and identifies palmitoylated proteins from complex protein extracts is described. Using the fatty acid exchange labeling chemistry (described in the preceding report), palmitoyl modifications are exchanged for biotinylated compounds, allowing the subset of palmitoyl-proteins to be affinity-purified and then identified by mass spectroscopic protein identification technologies. The advantages and pitfalls of this new technology are discussed within the context of the recent application of this method in the yeast Saccharomyces cerevisiae.
authors.affiliation: Department of Pharmacology, Wayne State University School of Medicine, Elliman Building, Room 1224, 421 E. Canfield, Detroit, MI 48201, USA.
authors.forename: Amy F
authors.initials: AF
authors.lastname: Roth
authors.forename: Junmei
authors.initials: J
authors.lastname: Wan
authors.forename: William N
authors.initials: WN
authors.lastname: Green
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Nicholas G
authors.initials: NG
authors.lastname: Davis
mesh_terms: D000478:Alkylation; D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D019308:Palmitic Acid / Q000032:analysis* / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012441:Saccharomyces cerevisiae; D014166:Transferases / Q000502:physiology
chemical_list: D011506:Proteins; D020543:Proteome; D019308:Palmitic Acid; D014166:Transferases