Attenuation of brain inflammatory response after focal cerebral ischemia/reperfusion with Xuesaitong injection in rats.
OBJECTIVE
To investigate the neuro-protective effect of Xuesaitong Injection (XST) on brain inflammatory response after transient focal cerebral ischemia/reperfusion in rats.


METHODS
Focal cerebral ischemia/reperfusion models of male rats were induced by transient occlusion for 2 h of middle cerebral artery (MCA) which was followed by 24 h reperfusion. XST was administered through intraperitoneal injection of 25 mg/kg or 50 mg/kg at 4 h after the onset of ischemia. After reperfusion for 24 h, the neurological function score was evaluated, the brain edema was detected with dry-wet weight method, the myeloperoxidase (MPO) activity and the expression of intercellular adhesion molecule-1 (ICAM-1) of ischemic cerebral cortex and caudate putamen was determined by spectrophotometry and immunohistochemistry respectively.


RESULTS
XST not only lowered neurological function score at the dose of 50 mg/kg, but reduced brain edema and inhibited MPO activity and ICAM-1 expression as compared with the ischemia/reperfusion model group (P < 0.01).


CONCLUSIONS
XST has a definite effect on inhibiting the expression of ICAM-1 and neutrophil infiltration in rats with cerebral ischemia/reperfusion when treatment started at 4 h after ischemia onset, and also attenuates inflammation in the infarcted cerebral area.
He
Wei
W
Department of Pharmacology, Gannan Medical College, Jiangxi, China. hewei8286291@yahoo.com.cn
Xu
Xiao-jun
XJ
D000818:Animals; D001929:Brain Edema / Q000188:drug therapy / Q000276:immunology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000276:immunology; D004365:Drugs, Chinese Herbal; D004660:Encephalitis / Q000188:drug therapy / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009504:Neutrophils / Q000276:immunology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000276:immunology; D012503:Saponins / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D012503:Saponins; C509799:xuesetong; D018799:Intercellular Adhesion Molecule-1; D009195:Peroxidase