title: Effect of heparin on blood vascular endothelial growth factor levels in patients with ST-elevation acute myocardial infarction undergoing primary percutaneous coronary intervention.
abstract: It has been demonstrated that high blood vascular endothelial growth factor (VEGF) levels in patients with myocardial infarction decrease rapidly after reperfusion, possibly in response to heparin administration. We measured serum VEGF concentration before and after heparin infusion in 105 patients with ST-elevation acute myocardial infarction (STEMI) who underwent primary percutaneous coronary intervention (PCI). Serum VEGF concentration in patients with STEMI was significantly higher than in healthy controls. After PCI, the concentration of VEGF decreased by approximately 70%, with the greatest decrease seen in patients with the highest initial VEGF levels. To determine whether heparin could decrease VEGF concentration by sequestering VEGF in the endothelium, a fixed dose of recombinant VEGF was incubated for 40 minutes with EA.hy926 endothelial cells in vitro. Recovery of VEGF from medium after culture was decreased by up to 15% with increasing doses of heparin. Concentration of VEGF did not change in the absence of heparin and/or endothelial cells. In conclusion, these results suggest that a rapid decrease in blood VEGF after PCI may be related to the administration of heparin, which binds simultaneously to VEGF and endothelial cells.
authors.affiliation: 1st Department of Cardiology, University Medical School, Poznań, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pyda
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Korybalska
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Ksiazek
authors.forename: Stefan
authors.initials: S
authors.lastname: Grajek
authors.forename: Magdalena
authors.initials: M
authors.lastname: Lanocha
authors.forename: Maciej
authors.initials: M
authors.lastname: Lesiak
authors.forename: Justyna
authors.initials: J
authors.lastname: Wiśniewska-Elnur
authors.forename: Anna
authors.initials: A
authors.lastname: Olasińska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Breborowicz
authors.forename: Andrzej
authors.initials: A
authors.lastname: Cieśliński
authors.forename: Janusz
authors.initials: J
authors.lastname: Witowski
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015906:Angioplasty, Balloon, Coronary; D000925:Anticoagulants / Q000494:pharmacology*; D016022:Case-Control Studies; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000628:therapy*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood* / Q000187:drug effects
chemical_list: D000925:Anticoagulants; D042461:Vascular Endothelial Growth Factor A; D006493:Heparin