title: Idiopathic pulmonary arterial hypertension in Dutch Caucasian patients is not associated with human herpes virus-8 infection.
abstract: Samples of lung tissue, taken at time of lung transplant, from 13 Dutch Caucasian patients with idiopathic pulmonary arterial hypertension (iPAH) and 14 patients with non-idiopathic PAH were studied for the presence of human herpes virus-8 (HHV-8). By immunohistochemical staining, in none of patients expression of HHV-8 latency-associated nuclear antigen 1 (LANA-1) was demonstrated. Using two nested polymerase chain reactions (PCR) to amplify part of the open reading frame (ORF) 65 and ORF 73, we failed to detect HHV-8 DNA in all samples studied. These results argue strongly against a role for HHV-8 in the pathogenesis of iPAH.
authors.affiliation: Department of Pulmonology, F5-144 Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands. p.bresser@amc.uva.nl
authors.forename: Paul
authors.initials: P
authors.lastname: Bresser
authors.forename: Marion I
authors.initials: MI
authors.lastname: Cornelissen
authors.forename: Wim
authors.initials: W
authors.lastname: van der Bij
authors.forename: Carel J M
authors.initials: CJ
authors.lastname: van Noesel
authors.forename: Wim
authors.initials: W
authors.lastname: Timens
mesh_terms: D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D006566:Herpesviridae Infections / Q000150:complications* / Q000208:ethnology; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D006976:Hypertension, Pulmonary / Q000150:complications* / Q000208:ethnology / Q000821:virology; D007150:Immunohistochemistry / Q000379:methods; D008168:Lung / Q000821:virology; D009687:Nuclear Proteins / Q000032:analysis; D044465:White People
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D009687:Nuclear Proteins; C109540:latency-associated nuclear antigen