title: Effects of metals and detergents on biotransformation and detoxification enzymes of leaping mullet (Liza saliens).
abstract: In this study, feral leaping mullet (Liza saliens) liver microsomal 7-ethoxyresorufin O-deethylase (EROD), and cytosolic glutathione S-transferases (GSTs) activities were investigated using 7-ethoxyresorufin, 1-chloro-2,4-dinitrobenzene (CDNB), and ethacrynic acid (EA) as substrates, respectively. The average EROD activity was found as 1139+/-175 pmol resorufin/min/mg protein. The average GST activities towards CDNB and EA were found as 1364+/-41 and 140+/-19 nmol/min/mg protein, respectively. We have, then, investigated the in vitro effects of some metals and detergents on CYP1A and GST activities in leaping mullet liver. Leaping mullet liver microsomal EROD activity was significantly inhibited by Hg (0.1 mM), Ni (0.1 mM), Cd (0.1 mM), Cu (0.1 mM), Zn (0.1 mM), Sb (0.1 mM), Fe2+ (1 mM), Co (1 mM), Al (1 mM), and Fe3+ (1 mM), with the percent inhibition of 80, 80, 77, 75, 70, 69, 56, 53, 46, and 44, respectively. Similarly, conjugation of CDNB catalyzed by GST was inhibited significantly to lesser extend by Hg (0.1mM), Sb (0.1 mM), Cd (0.1 mM), Cu (0.1 mM), Zn (0.1 mM), Fe3+ (1 mM), Co (1 mM), and Fe2+ (1 mM), with the percent inhibition of 70, 69, 65, 61, 54, 51, 47, and 43, respectively. The degrees of inhibition observed on GST catalyzed EA conjugation by Hg (0.1 mM), Cd (0.1 mM), Sb (0.1 mM), Cu (0.1 mM), and Zn (0.1 mM) were 86, 78, 69, 51, and 42, respectively. In addition to metals, the effect of various detergents on leaping mullet liver EROD, GST-CDNB, and GST-EA activities were studied. It was found that ionic detergents strongly inhibited the EROD activity, whereas much less inhibitions were observed with GST catalyzed activities. Therefore, the CYP1A inhibition potencies of metals and detergents suggest that their contribution to the overall CYP1A induction in polycyclic aromatic hydrocarbons contaminated environmental samples has to be taken into account for better interpretation of environmental studies.
authors.affiliation: Department of Biology, Pamukkale University, Kinikli-Denizli 20017, Turkey. sena@pamukkale.edu.tr
authors.forename: Alaattin
authors.initials: A
authors.lastname: Sen
authors.forename: Asli
authors.initials: A
authors.lastname: Semiz
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001711:Biotransformation; D019363:Cytochrome P-450 CYP1A1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D003902:Detergents / Q000633:toxicity*; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D004791:Enzyme Inhibitors / Q000633:toxicity*; D004976:Ethacrynic Acid / Q000378:metabolism; D029941:Fish Proteins / Q000378:metabolism*; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008658:Inactivation, Metabolic; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008670:Metals / Q000633:toxicity*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D010078:Oxazines / Q000378:metabolism; D023701:Smegmamorpha / Q000378:metabolism; D013379:Substrate Specificity; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D003902:Detergents; D004137:Dinitrochlorobenzene; D004791:Enzyme Inhibitors; D029941:Fish Proteins; D008670:Metals; D010078:Oxazines; D014874:Water Pollutants, Chemical; C007649:ethoxyresorufin; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase; D004976:Ethacrynic Acid