[Construction of multifunctonal nano-delivery system crossing blood brain barrier].
OBJECTIVE
To construct multifunctonal nano-delivery system crossing the blood brain barrier (BBB).


METHODS
The magnetic nanoparticles were preprared with O-carboxylmethylated chitosan (O-CMC) and conjugated with a peptide sequence from the human immunodeficiency virus 1-tat protein and transferrin (Tf), and anti-tumor drug methotrexate (MTX), and thus constructed a O-CMC magnetic nanoparticles carrier system conjugating with Tat and Tf (O-MNPs-Tat-Tf) that combines multiple functions including crossing BBB, magnetism, receptor-mediated dual targets and anti-tumor capabilities. The appearance, diameter, and magnetism of MTX-O-MNPs-Tat-Tf carrier system were characterized with transmission electronic microscopy, atomic force microscopy and vibrating samples magnetometer. The cytotoxicity of MTX-loaded O-MNPs-Tat-Tf was investigated with C6 glioma cells. The ability of O-MNPs-Tat-Tf crossing BBB was investigated in rats by single photon emission computed tomography.


RESULTS
The mean particle diameter was 75 nm, along with good anti-tumor property. The multi-functioned carrier system successfully crossed the BBB in rat.


CONCLUSIONS
The establishment of MTX-O-MNPs-Tat-Tf carrier model implies a promising future for its application in therapy of cerebral diseases.
Yao
Peng
P
Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China.
Huang
Jie
J
Kang
Chun-Sheng
CS
Pu
Pei-Yu
PY
Chang
Jin
J
D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism; D048271:Chitosan / Q000031:analogs & derivatives / Q000737:chemistry; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006801:Humans; D008280:Magnetics; D053758:Nanoparticles; D014168:Transferrin
D004337:Drug Carriers; C504164:O-carboxymethylchitosan; D014168:Transferrin; D048271:Chitosan