title: [Effect of hyaluronic acid chitosan-based microemulsion on the permeability of blood brain barrier in mice].
abstract: OBJECTIVE
  To study the effect of hyaluronic acid chitosan-based microemulsion (HAC-ME) on the permeability of blood brain barrier( BBB) by using Evans blue (EB) as the indicator.
  
  
  METHODS
  A formamide extraction-ultraviolet spectrophotometry method was employed to determine the concentrations of EB in each of the tissues. The in vivo distribution of HAC-ME groups containing EB in mice and the fluorescence intensity and diffusion domain of brain slices were all studied.
  
  
  RESULTS
  Contrasting to the common microemulsion (ME), HAC-ME at the lower concentration of HAC (<5 mg x mL(-1)) could further improve the transporting of EB across the BBB while EB concentration in other tissues decreased, and Tmax was delayed about 30 min.
  
  
  CONCLUSIONS
  HAC-ME could facilitate the transporting of EB across the BBB and it was concentration dependent. While the brain targeting absorptive capability of HAC-ME was enhanced.
authors.affiliation: Department of Pharmaceutics, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yao
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Zhou
authors.forename: Qi-neng
authors.initials: QN
authors.lastname: Ping
authors.forename: Yun
authors.initials: Y
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D005070:Evans Blue / Q000737:chemistry / Q000493:pharmacokinetics; D005260:Female; D006820:Hyaluronic Acid / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010316:Particle Size; D014018:Tissue Distribution
chemical_list: D004655:Emulsions; D005070:Evans Blue; D006820:Hyaluronic Acid; D048271:Chitosan