Cytokine-induced killer T cells kill immature dendritic cells by TCR-independent and perforin-dependent mechanisms.
Cytokine-induced killer (CIK) cells are ex vivo, expanded T cells with proven anticancer activity in vitro and in vivo. However, their functional properties with the exception of their cancer cell-killing activity are largely unclear. Here, we show that CIK T cells recognize dendritic cells (DC), and although mature DC (mDC) induce CIK T cells to produce IFN-gamma, immature DC (iDC) are killed selectively by them. Moreover, CIK T cell activation by mDC and their destruction of iDC are independent of the TCR. The cytotoxicity of CIK T cells to iDC is perforin-dependent. Our data have revealed an important regulatory role of CIK cells.
Joshi
Pramod S
PS
Department of Pathology and Comprehensive Cancer Center, The Ohio State University Medical Center, 129 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210, USA.
Liu
Jin-Qing
JQ
Wang
Yin
Y
Chang
Xing
X
Richards
John
J
Assarsson
Erika
E
Shi
Fu-Dong
FD
Ljunggren
Hans-Gustaf
HG
Bai
Xue-Feng
XF
D000818:Animals; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells; D007371:Interferon-gamma / Q000276:immunology*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D009369:Neoplasms / Q000276:immunology; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D011948:Receptors, Antigen, T-Cell; D054353:Perforin; D007371:Interferon-gamma