Cartilaginous tissue formation from bone marrow cells using rotating wall vessel (RWV) bioreactor.
This is the first successful report of the rapid regeneration of three-dimensional large and homogeneous cartilaginous tissue from rabbit bone marrow cells without a scaffold using a rotating wall vessel (RWV) bioreactor, which simulates a microgravity environment for cells. Bone marrow cells cultured for 3 weeks in DMEM were resuspended and cultured for 4 weeks in the chondrogenic medium within the vessel. Large cylindrical cartilaginous tissue with dimensions of (1.25 +/- 0.06) x (0.60 +/- 0.08) cm (height x diameter) formed. Their cartilage marker expression was confirmed by mRNA expressions of aggrecan, collagen type I and II, and glycosaminoglycan (GAG)/DNA ratio. Their cartilaginous properties were demonstrated by toluidine blue, safranin-O staining, and polarization.
Ohyabu
Yoshimi
Y
Nanotechnology Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba Central-6, Tsukuba, Ibaraki 305-8566, Japan.
Kida
Naoko
N
Kojima
Hiroko
H
Taguchi
Tetsushi
T
Tanaka
Junzo
J
Uemura
Toshimasa
T
D000818:Animals; D019149:Bioreactors; D001854:Bone Marrow Cells / Q000502:physiology*; D002356:Cartilage / Q000254:growth & development* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D020869:Gene Expression Profiling; D011817:Rabbits; D046509:Tissue Culture Techniques / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*; D014893:Weightlessness
D016326:Extracellular Matrix Proteins