title: The effect of taurine on renal ischemia/reperfusion injury.
abstract: Ischemia-reperfusion (I/R) injury is one of the most common causes of renal dysfunction. Taurine is an endogenous antioxidant and a membrane-stabilizing, intracellular, free beta-amino acid. It has been demonstrated to have protective effects against I/R injuries to tissues other than kidney. The aim of this study was to determine whether taurine has a beneficial role in renal I/R injury. Forty Wistar-Albino rats were allocated into four groups as follows: sham, taurine, I/R, and I/R+taurine. Taurine 7.5 mg/kg was given intra-peritoneally to rats in the groups taurine and I/R+taurine. Renal I/R was achieved by occluding the renal arteries bilaterally for 40 min, followed by 6 h of reperfusion. Immediately thereafter, blood was drawn and tissue samples were harvested to measure 1) serum levels of BUN and creatinine; 2) serum and/or tissue levels of malondialdehyde (MDA), glutathione (GSH), glucose 6-phosphate dehydrogenase (G-6PD), 6-phosphogluconate dehydrogenase (6-PGD) and glutathione reductase (GSH-red); 3) renal morphology; and 4) immunohistochemical staining for P-selectin. Taurine administration reduced I/R-induced increases in serum BUN and creatinine, and serum and tissue MDA levels (p<0.05). Additionally, taurine lessened the reductions in serum and tissue glutathione levels secondary to I/R (p<0.05). Taurine also attenuated histopathologic evidence of renal injury, and reduced I/R-induced P-selectin immunoreactivity (p<0.05). Overall, then, taurine administration appears to reduce the injurious effects of I/R on kidney.
authors.affiliation: Department of Nephrology, Gazi University Faculty of Medicine, Ankara, Turkey. galip_guz@hotmail.com
authors.forename: G
authors.initials: G
authors.lastname: Guz
authors.forename: E
authors.initials: E
authors.lastname: Oz
authors.forename: N
authors.initials: N
authors.lastname: Lortlar
authors.forename: N N
authors.initials: NN
authors.lastname: Ulusu
authors.forename: N
authors.initials: N
authors.lastname: Nurlu
authors.forename: B
authors.initials: B
authors.lastname: Demirogullari
authors.forename: S
authors.initials: S
authors.lastname: Omeroglu
authors.forename: S
authors.initials: S
authors.lastname: Sert
authors.forename: C
authors.initials: C
authors.lastname: Karasu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D015415:Biomarkers / Q000097:blood; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D013654:Taurine / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D013654:Taurine