Sca-1 and Thy-1 accelerate neuron-like differentiation in bone marrow stromal cells.
Bone marrow stromal cells taken from EGFP transgenic mice were sorted by magnetic beads with surface markers for Sca-1 and Thy-1. The cells were then co-cultured on organotypic hippocampal slice or with neuronal cell feeder in dish. On hippocampus, both Sca-1 and Thy-1 positive cells showed 4- 8 folds higher potential to show neuron-like morphology than negative cells. In dish, negative cells fewly survived but each positive cells survived and showed neuron-like differentiation. In both culture condition, retinoic acid supplement accelerate differentiation. Differentiated Sca-1 and Thy-1 positive cells were immunohistochemically GFAP- and NeuN-negative but nestin-, neurofilament- and NSE-positive. Neuron-like differentiation of bone marrow cells can be enhanced by selection using cell surface proteins.
Mizobe
Takashi
T
Department of Neurosurgery, Kobe University Graduate School of Medicine.
Kidoguchi
Keiji
K
Tamaki
Masahiro
M
Sasayama
Takashi
T
Kondoh
Takeshi
T
Kohmura
Eiji
E
D000818:Animals; D000950:Antigens, Ly / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D017930:Genes, Reporter; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D018800:Thy-1 Antigens / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D018800:Thy-1 Antigens