title: mTOR signaling in the hippocampus is necessary for memory formation.
abstract: It is widely accepted that the formation of long-term memory (LTM) requires mRNA translation, but little is known about the cellular mechanisms in the brain that regulate this process. Mammalian target of rapamycin (mTOR) is a key regulator of translational efficacy and capacity. Here, we show that LTM formation of one-trial inhibitory avoidance (IA) in rats, a hippocampus-dependent fear-motivated learning task, requires mTOR activation. IA training is specifically associated with a rapid increase in the phosphorylation state of mTOR and its substrate ribosomal S6 kinase (p70S6K). Bilateral intra-CA1 infusion of rapamycin, a selective mTOR inhibitor, 15 min before, but not immediately after training completely hinders IA LTM without affecting short-term memory (STM) retention. Therefore, our findings indicate that the regulation of hippocampal mRNA translation is a major control step in memory consolidation.
authors.affiliation: Instituto de Biología Celular y Neurociencias, Facultad de Medicina, UBA, Buenos Aires, Argentina.
authors.forename: Pedro
authors.initials: P
authors.lastname: Bekinschtein
authors.forename: Cynthia
authors.initials: C
authors.lastname: Katche
authors.forename: Leandro N
authors.initials: LN
authors.lastname: Slipczuk
authors.forename: Lionel Müller
authors.initials: LM
authors.lastname: Igaz
authors.forename: Martín
authors.initials: M
authors.lastname: Cammarota
authors.forename: Iván
authors.initials: I
authors.lastname: Izquierdo
authors.forename: Jorge H
authors.initials: JH
authors.lastname: Medina
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology; D001522:Behavior, Animal; D015153:Blotting, Western; D006624:Hippocampus / Q000502:physiology*; D008297:Male; D008568:Memory / Q000502:physiology*; D010766:Phosphorylation / Q000187:drug effects; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000187:drug effects; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases
chemical_list: D011494:Protein Kinases; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus