piggyBac is a flexible and highly active transposon as compared to sleeping beauty, Tol2, and Mos1 in mammalian cells.
A nonviral vector for highly efficient site-specific integration would be desirable for many applications in transgenesis, including gene therapy. In this study we directly compared the genomic integration efficiencies of piggyBac, hyperactive Sleeping Beauty (SB11), Tol2, and Mos1 in four mammalian cell lines. piggyBac demonstrated significantly higher transposition activity in all cell lines whereas Mos1 had no activity. Furthermore, piggyBac transposase coupled to the GAL4 DNA-binding domain retains transposition activity whereas similarly manipulated gene products of Tol2 and SB11 were inactive. The high transposition activity of piggyBac and the flexibility for molecular modification of its transposase suggest the possibility of using it routinely for mammalian transgenesis.
Wu
Sareina Chiung-Yuan
SC
Cancer Research Center, Medical College of Georgia, Augusta, GA 30912, USA.
Meir
Yaa-Jyuhn James
YJ
Coates
Craig J
CJ
Handler
Alfred M
AM
Pelczar
Pawel
P
Moisyadi
Stefan
S
Kaminski
Joseph M
JM
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D004251:DNA Transposable Elements / Q000235:genetics* / Q000502:physiology; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D019476:Insect Proteins / Q000235:genetics* / Q000502:physiology; D009036:Moths / Q000821:virology*; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed; D019895:Transposases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D019476:Insect Proteins; C111652:mariner transposases; D019895:Transposases; C494471:sleeping beauty transposase, human