Confirmation of sex-specific transcripts from Ancylostoma caninum adult worms by semi-quantitative RT-PCR.
In a previous work Ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific RT-PCR comparing male and female cDNA. In this work more fragments had been identified and a semi-quantitative RT-PCR carried out in order to confirm the sex-specific character of the transcripts obtained. Four fragments were confirmed as sex-specific or as being expressed more in one sex than the other.
Miranda
R R
RR
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
Clara e Silva
L
L
Santos
H A
HA
Rabelo
E M
EM
D000722:Ancylostoma / Q000235:genetics* / Q000302:isolation & purification; D000724:Ancylostomiasis / Q000469:parasitology / Q000662:veterinary*; D000818:Animals; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D005260:Female; D008297:Male; D018510:RNA, Helminth / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D020539:Sequence Analysis, Protein; D012727:Sex Characteristics
D018510:RNA, Helminth