Plant proteome analysis: a 2004-2006 update.
Since the appearance of the review entitled "Plant Proteome Analysis" in Proteomics in February 2004 (Cánovas, F. M., Dumas-Gaudot, E., Recorbert, G., Jorrín, J. et al., Proteomics 2004, 4, 285-298), about 200 original articles focusing on plant proteomics have been published. Although this represents less than 1% of the global proteomics output during this period, it nevertheless reflects an increase in activity over the period 1999-2004. These papers concern the proteome of at least 35 plant species but have concentrated mainly on thale cress (Arabidopsis thaliana) and rice (Oryza sativa). The scientific objectives have ranged from a proteomic analysis of organs, tissues, cell suspensions, or subcellular fractions to the study of plant development and response to various stresses. A number of contributions have covered PTMs and protein interactions. The dominant analytical platform has been 2-DE coupled to MS, but "second generation" techniques such as DIGE, multidimensional protein identification technology, isotope-coded affinity tags, and stable isotope labeling by amino acids in cell culture have begun to make an impact. This review aims to provide an update of the contribution of proteomics to plant biology during the period 2004-2006, and is divided into six sections: introduction, subcellular proteomes, plant development, responses to biotic and abiotic stresses, PTMs, and protein interactions. The conclusions summarize a view of the major pitfalls and challenges of plant proteomics.
Rossignol
Michel
M
UR 1199, Protéomique, INRA, Montpellier, France.
Peltier
Jean-Benoît
JB
Mock
Hans-Peter
HP
Matros
Andrea
A
Maldonado
Ana M
AM
Jorrín
Jesús V
JV
D017360:Arabidopsis / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D012275:Oryza / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry*; D025941:Protein Interaction Mapping; D011499:Protein Processing, Post-Translational; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D013347:Subcellular Fractions / Q000378:metabolism
D010940:Plant Proteins; D020543:Proteome