title: Detection and modelling of DNA hybridization by EIS measurements. Mention of a polythiophene matrix suitable for electrochemically controlled gene delivery.
abstract: A conducting polymer sensor for direct label-free DNA detection based on a polythiophene bearing an electroactive linker group is investigated. DNA hybridization is studied by electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance (QCM) techniques. Modelling of DNA hybridization by EIS measurements exhibits the contribution of nucleic acid to a superficial p-doping process. A 675-mer single-stranded DNA is produced using asymmetric PCR from a DNA sequence of a transposable element mariner and hybridized to the previously immobilized probe. Electrochemical stimulus leads to the release "on demand" of DNA fragments and the amount delivery permits to do PCR amplification.
authors.affiliation: L.C.O.M. Chimie des Polymères (UCO2M, UMR CNRS 6011), Université du Maine, Avenue O. Messiaen, 72085 Le Mans Cedex 9, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Gautier
authors.forename: Charles
authors.initials: C
authors.lastname: Cougnon
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Pilard
authors.forename: Nathalie
authors.initials: N
authors.lastname: Casse
authors.forename: Benoît
authors.initials: B
authors.lastname: Chénais
authors.forename: Marc
authors.initials: M
authors.lastname: Laulier
mesh_terms: D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D018014:Gene Transfer Techniques; D009693:Nucleic Acid Hybridization; D011108:Polymers; D013057:Spectrum Analysis; D013876:Thiophenes
chemical_list: D011108:Polymers; D013876:Thiophenes; C066730:polythiophene; D004247:DNA