title: Expression of aromatase and 5-alpha-reductase genes in endometrial adenocarcinoma.
abstract: The aim of this study was to investigate the expression of genes coding aromatase and 5-alpha-reductase type 1 and type 2 in endometrial adenocarcinoma, with parallel analysis of testosterone concentrations in serum isolated from blood of basilic and ovarian vein. A significant difference between the copy numbers of mRNA for 5-alpha-reductase type 1 and type 2 was disclosed. The content of the mRNA copies was higher for 5-alpha-reductase type 2 than for type 1. We noted a positive correlation between the mRNA copy number for aromatase and 5-alpha-reductase type 1 as well as between the mRNA copy number for aromatase and 5-alpha-reductase type 2. A positive correlation was disclosed between the mRNA copy number for 5-alpha-reductase type 1 and type 2. We concluded that in tissues of endometrial adenocarcinoma, there is expression of aromatase and 5-alpha-reductase type 1 and 2 genes, and that the expression of aromatase and 5-alpha-reductase genes in tissues of endometrial adenocarcinoma is not controlled by testosterone.
authors.affiliation: Department of Perinatology and Gynecology, Medical University of Silesia, Plac Traugutta 6, 41-800 Zabrze, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kazmierczak
authors.forename: Piotr
authors.initials: P
authors.lastname: Skalba
authors.forename: Anna
authors.initials: A
authors.lastname: Dabkowska-Huc
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Samojedny
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000235:genetics*; D000230:Adenocarcinoma / Q000097:blood* / Q000235:genetics*; D001141:Aromatase / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000097:blood* / Q000235:genetics*; D005260:Female; D018628:Gene Dosage; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D012333:RNA, Messenger; D013739:Testosterone / Q000097:blood
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D013739:Testosterone; D001141:Aromatase; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase