title: The YCR079w gene confers a rapamycin-resistant function and encodes the sixth type 2C protein phosphatase in Saccharomyces cerevisiae.
abstract: Type 2C protein phosphatase (PP2C) is a monomeric enzyme and requires Mg(2+) or Mn(2+) for its activity. Up to now, seven PP2C-like genes have been identified in the genome of Saccharomyces cerevisiae. However, the protein encoded by the sixth PP2C-like gene, YCR079w, has not been demonstrated to have PP2C activity. In this study, we show that YCR079w confers a rapamycin-resistant function in yeast cells, and we also demonstrate that the YCR079w-encoded protein exhibits characteristics of a typical PP2C. Therefore, YCR079w encodes the sixth PP2C, PTC6, in budding yeast.
authors.affiliation: Laboratory of Molecular Microbiology, Department of Molecular and Cellular Pharmacology, College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin, China.
authors.forename: Haihua
authors.initials: H
authors.lastname: Ruan
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Yan
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.forename: Linghuo
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology*; D025141:Drug Resistance, Fungal / Q000235:genetics*; D017353:Gene Deletion; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020123:Sirolimus / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D029701:Saccharomyces cerevisiae Proteins; D010749:Phosphoprotein Phosphatases; D020123:Sirolimus