title: Neonatal administration of diethylstilbestrol has adverse effects on somatic cells rather than germ cells.
abstract: Neonatal administration of diethylstilbestrol (DES) to rodents has adverse effects on spermatogenesis. However, not many studies have been conducted to determine which type of cell - germ or somatic - is the major target of DES. In order to clarify this, we tried reciprocal germ cell transplantation--transplantation of germ cells from DES-treated mice into intact mice and germ cells from normal mice into DES-treated mice. The donor germ cells were tagged with the green fluorescent protein (GFP) gene in order to distinguish the exogenous germ cells from the endogenous cells. Moreover, to obtain a large number of spermatogonia from the testes of adult mice, we performed fractionation by centrifugation with Percoll. Consequently, we found that the germ cells collected from DES-treated mice have differentiated into normal sperms in normal seminiferous tubules. However, in the case of the transplantation of normal germ cells into the seminiferous tubules of DES-treated mice, defective spermatogenesis was observed. In conclusion, DES has adverse effects on the somatic cells that are involved in spermatogenesis rather than the germ cells.
authors.affiliation: Department of Bioenvironmental Medicine (A3), Graduate School of Medicine, Chiba University, Chiba, Japan.
authors.forename: Kyu-Bom
authors.initials: KB
authors.lastname: Koh
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Toyama
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Komiyama
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Adachi
authors.forename: Hideki
authors.initials: H
authors.lastname: Fukata
authors.forename: Chisato
authors.initials: C
authors.lastname: Mori
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity; D002066:Busulfan / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects; D004054:Diethylstilbestrol / Q000008:administration & dosage / Q000633:toxicity*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence / Q000379:methods; D012671:Seminiferous Tubules / Q000166:cytology / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013093:Spermatogonia / Q000166:cytology / Q000187:drug effects* / Q000637:transplantation; D033581:Stem Cell Transplantation / Q000379:methods; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000637:transplantation
chemical_list: D018906:Antineoplastic Agents, Alkylating; D049452:Green Fluorescent Proteins; D004054:Diethylstilbestrol; D002066:Busulfan