title: Synthesis of RNA using 2'-O-DTM protection.
abstract: tert-Butyldithiomethyl (DTM), a novel hydroxyl protecting group, cleavable under reductive conditions, was developed and applied for the protection of 2'-OH during solid-phase RNA synthesis. This function is compatible with all standard protecting groups used in oligonucleotide synthesis, and allows for fast and high-yield synthesis of RNA. Oligonucleotides containing the 2'-O-DTM groups can be easily deprotected under the mildest possible aqueous and homogeneous conditions. The preserved 5'-O-DMTr function can be used for high-throughput cartridge RNA purification.
authors.affiliation: Department of Genetics and Pathology, Uppsala University, Sweden.
authors.forename: Andrey
authors.initials: A
authors.lastname: Semenyuk
authors.forename: Andras
authors.initials: A
authors.lastname: Földesi
authors.forename: Tommy
authors.initials: T
authors.lastname: Johansson
authors.forename: Camilla
authors.initials: C
authors.lastname: Estmer-Nilsson
authors.forename: Peter
authors.initials: P
authors.lastname: Blomgren
authors.forename: Mathias
authors.initials: M
authors.lastname: Brännvall
authors.forename: Leif A
authors.initials: LA
authors.lastname: Kirsebom
authors.forename: Marek
authors.initials: M
authors.lastname: Kwiatkowski
mesh_terms: D000473:Alkanes / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000138:chemical synthesis* / Q000302:isolation & purification; D013457:Sulfur Compounds / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D000473:Alkanes; D013457:Sulfur Compounds; D012313:RNA