title: Reduction in antimicrobial substantivity of MTAD after initial sodium hypochlorite irrigation.
abstract: Potential intrinsic tetracycline staining of intraradicular dentin has been observed when BioPure MTAD was employed as the final irrigant after initial rinsing with NaOCl. This study examined the effect of NaOCl-MTAD interaction on the antimicrobial substantivity of MTAD in dentin. Dentin cores previously irrigated with either MTAD, or in conjunction with 1.3% NaOCl as an initial irrigant were placed on blood agar plates inoculated with Escherichia faecalis at 10(5) cfu/ml. Dentin cores irrigated with 1.3% NaOCl only, and autoclaved dentin disks were used as the respective positive and negative controls. After anaerobic incubation, the mean diameter of bacterial inhibition zones formed around the MTAD group was significantly larger than the NaOCl/MTAD group, which, in turn, was not significantly different from the NaOCl positive control. Oxidation of MTAD by NaOCl resulted in the partial loss of antimicrobial substantivity in a manner similar to the peroxidation of tetracycline by reactive oxygen species.
authors.affiliation: Department of Oral Biology and Maxillofacial Pathology, Medical College of Georgia, Augusta, Georgia 30912-1129, USA. franklintay@gmail.com
authors.forename: Franklin R
authors.initials: FR
authors.lastname: Tay
authors.forename: Noriko
authors.initials: N
authors.lastname: Hiraishi
authors.forename: George S
authors.initials: GS
authors.lastname: Schuster
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Loushine
authors.forename: Hani F
authors.initials: HF
authors.lastname: Ounsi
authors.forename: Simone
authors.initials: S
authors.lastname: Grandini
authors.forename: Joyce Y Y
authors.initials: JY
authors.lastname: Yau
authors.forename: Annalisa
authors.initials: A
authors.lastname: Mazzoni
authors.forename: Adam
authors.initials: A
authors.lastname: Donnelly
authors.forename: Nigel M
authors.initials: NM
authors.lastname: King
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D019343:Citric Acid / Q000494:pharmacology*; D003094:Collagen / Q000648:ultrastructure; D015169:Colony Count, Microbial; D003804:Dentin / Q000187:drug effects / Q000382:microbiology; D004318:Doxycycline / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D004336:Drug Antagonism; D004338:Drug Combinations; D004925:Escherichia / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D011136:Polysorbates / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000008:administration & dosage / Q000494:pharmacology*; D016369:Smear Layer; D012973:Sodium Hypochlorite / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D004338:Drug Combinations; C475043:MTAD (intracanal irrigant); D011136:Polysorbates; D012388:Root Canal Irrigants; D019343:Citric Acid; D003094:Collagen; D012973:Sodium Hypochlorite; D004318:Doxycycline