title: Clonal analysis of Inquilinus limosus isolates from six cystic fibrosis patients and specific serum antibody response.
abstract: Inquilinus limosus is a novel Gram-negative bacterium of the subdivision alpha-Proteobacteria recently found in the airways of patients with cystic fibrosis (CF). Here, the authors report on the clinical courses of six CF patients colonized with I. limosus. Five patients suffered from either an acute respiratory exacerbation or a progressive loss of pulmonary function, whereas one patient was in a stable clinical situation. This study focused on two aims: (i) the clonal analysis of I. limosus isolates by random amplified polymorphic DNA (RAPD)-PCR, and (ii) the clarification of whether the presence of I. limosus in the respiratory tract is associated with a specific serum antibody response. Serum IgG was detected by immunoblotting using I. limosus whole-cell-lysate proteins as antigens. Sera from healthy blood donors (n=10) and from CF patients colonized with Pseudomonas aeruginosa (n=10) were found to be immunoblot negative. All six Inquilinus-positive patients raised serum IgG antibodies against various I. limosus antigens. Surprisingly, in one patient, a specific I. limosus serum antibody response was already detected 1 year prior to Inquilinus-positive sputum cultures. Two prominent antigens were characterized by MALDI-MS: a 23 kDa protein revealed homology to the outer membrane lipoprotein OmlA of Actinobacillus pleuropneumoniae, and an 18 kDa protein to a protein-tyrosine phosphatase of Burkholderia cepacia. In conclusion, detection of I. limosus is accompanied by a specific serum antibody response and may reflect the infectious/pathogenic potential of I. limosus. Moreover, IgG immunoblotting may be useful to detect early infection with I. limosus and may support the selective cultivation of this novel emerging pathogen.
authors.affiliation: Max von Pettenkofer-Institute for Medical Microbiology, Ludwig-Maximilians-University, Pettenkoferstrasse 9a, 80336 Munich, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Schmoldt
authors.affiliation: Christiane-Herzog-Ambulanz, Dr. von Haunersches Kinderspital, Ludwig-Maximilians-University, Lindwurmstrasse 4, 80337 Munich, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Latzin
authors.affiliation: Max von Pettenkofer-Institute for Medical Microbiology, Ludwig-Maximilians-University, Pettenkoferstrasse 9a, 80336 Munich, Germany.
authors.forename: Juergen
authors.initials: J
authors.lastname: Heesemann
authors.affiliation: Christiane-Herzog-Ambulanz, Dr. von Haunersches Kinderspital, Ludwig-Maximilians-University, Lindwurmstrasse 4, 80337 Munich, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Griese
authors.affiliation: Protein Analysis Unit, Adolf-Butenandt-Institut, Ludwig-Maximilians-University, Schillerstrasse 44, 80336 Munich, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Imhof
authors.affiliation: Max von Pettenkofer-Institute for Medical Microbiology, Ludwig-Maximilians-University, Pettenkoferstrasse 9a, 80336 Munich, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hogardt
mesh_terms: D000293:Adolescent; D000328:Adult; D020561:Alphaproteobacteria / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000737:chemistry / Q000145:classification; D003550:Cystic Fibrosis / Q000097:blood* / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000097:blood* / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D008297:Male; D008970:Molecular Weight; D019105:Random Amplified Polymorphic DNA Technique; D012137:Respiratory System / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007074:Immunoglobulin G