[Effect of extract components from Plastrum testudinis and their combination component on proliferation of rat mesenchymal stem cell in vitro].
OBJECTIVE
To observe the effect of extract components from Plastrum Testudinis and their combination component on the proliferaiton of mesenchymal stem cell (MSC) to screen out the effective components.


METHODS
Mesenchymal stem cells were dissociated from bone marrow of rat and were marked by Brdu, and the expression of CD44, CD54 and double label of Brdu and CD44 extract components (A to approximately J) with different concentration (2 microl, 5 microl,10 microl,20 microl)added to cultured MSC. The cell viability was tested by MTT.


RESULTS
Cell viability in component A, B, H, I with 20 microl, C, F with 10 microl and J with 5 microl concentration group increased compared with that in control group (P < 0.05) and cell viability in compponent E with 2 microl to approximately 20 microl concentration groups decreased compared with that in control group (P < 0.05). Cell viability in component D and G with 2 microl to approximately 20 microl concentration groups was similar to that in control group. Cell viability in combination component with J component increased, but cell viability in combination component with E component decrased.


CONCLUSIONS
All compoents A, B,C,F, H, I and J can improve proliferation of MSC. Anong them, component J and its combination are the best. Component E and its combination can inhibit proliferation. But component D and G have no effect on proliferation of MSC.
Zhou
Jian-hong
JH
Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Zeng
He-ping
HP
Chen
Dong-feng
DF
Li
Hui
H
Li
Xi-can
XC
Li
Yi-wei
YW
Du
Shao-hui
SH
D000818:Animals; D001853:Bone Marrow; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D008297:Male; D008419:Materia Medica / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D008419:Materia Medica