Cryptotrichosporon anacardii gen. nov., sp. nov., a new trichosporonoid capsulate basidiomycetous yeast from Nigeria that is able to form melanin on niger seed agar.
Five yeast isolates obtained from cashew tree flowers in Nigeria resembled Cryptococcus neoformans phenotypically by producing brown pigmented colonies on niger seed agar, expressing a capsule, and being able to grow at 37 degrees C. However, rRNA gene sequences, including the 18S rRNA gene, the D1/D2 domains of the 26S rRNA gene and the ITS1+2 regions, suggested that these yeasts form a basal lineage within the Trichosporonales (Tremellomycetidae, Hymenomycetes, Basidiomycota, Fungi). Since the isolates could not be identified with any known genus and species within the Trichosporonales, we describe them as Cryptotrichosporon anacardii gen. et sp. nov. with CBS 9551(T) (=NRRL Y-27671) as the type strain. The taxonomic conflict between phenetic and molecular classification schemes within this group of fungi is discussed, and is resolved in favor of the latter.
Okoli
Ikechukwu
I
Department of Applied Microbiology, Nnamdi Azikiwe University, Anambra State, Nigeria.
Oyeka
Christie A
CA
Kwon-Chung
Kyung J
KJ
Theelen
Bart
B
Robert
Vincent
V
Groenewald
Johannes Z
JZ
McFadden
Diane C
DC
Casadevall
Arturo
A
Boekhout
Teun
T
D000362:Agar; D031021:Anacardium / Q000382:microbiology*; D019659:Asteraceae; D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D035264:Flowers / Q000382:microbiology*; D008543:Melanins / Q000378:metabolism*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D009549:Nigeria; D012335:RNA, Ribosomal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012639:Seeds; D017422:Sequence Analysis, DNA
D003470:Culture Media; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D008543:Melanins; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; C065096:RNA, ribosomal, 26S; D000362:Agar