Cryopreservation of cultivated and wild potato varieties by droplet vitrification: effect of subculture of mother-plants and of preculture of shoot tips.
In this paper, we studied the effect of subculture of mother-plants and of preculture of shoot tips of two potato varieties (Dejima, cultivated and STN13, wild) cryopreserved using the droplet-vitrification technique. The subculture conditions (light intensity, aeration and planting density) significantly affected survival of both non-cryopreserved and cryopreserved shoot-tips in both varieties. The subculture duration and the position of the shoot tips on the axis of the in vitro plantlets had a significant (P<0.0001) effect on survival of cryopreserved shoot tips. The optimal subculture duration was 7 and 5 weeks and the optimal size of shoot tips was 1.5-2.0 and 1.0-1.5 mm for var. Dejima and STN13, respectively. Survival of cryopreserved shoot tips was influenced by the sucrose concentration in the preculture medium and the preculture duration. The highest survival of cryopreserved shoot tips was observed after preculture with 0.3 M sucrose for 8 h followed by 0.7 M sucrose for 18 h. These results indicate that the parameters of the subculture of mother-plants and of preculture of shoot tips should be carefully optimized, especially in the case of wild species.
Yoon
Ju-Won
JW
National Institute of Agricultural Biotechnology, RDA, Suwon, Korea.
Kim
Haeng-Hoon
HH
Ko
Ho-Cheol
HC
Hwang
Hae-Sung
HS
Hong
Eun-Sun
ES
Cho
Eun-Gi
EG
Engelmann
Florent
F
D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods; D004305:Dose-Response Relationship, Drug; D018520:Plant Shoots / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D032322:Solanum / Q000166:cytology / Q000235:genetics* / Q000502:physiology*; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors
D003451:Cryoprotective Agents; D003470:Culture Media; D013395:Sucrose