Nature of DNA-bound fatty acids in Pseudomonas aurantiaca.
The existence of Pseudomonas aurantiaca DNA-bound fatty acids and lipids is presented in this work. The isolation of DNA was carried out by two different procedures, namely, phenol and detergent-based phenol isolation in order to prove the presence of DNA-bound lipids. The lipid content of DNA is expressed in terms of fatty acid profile. A high level of 16:0, 18:0 and 18:1 is characteristic for tightly bound DNA lipids. On the other hand, the fatty acids such as 14:1, iso14:0 and iso16:0 are found in trace amounts only in DNA lipid fraction, but these fatty acids are not found in the whole-cell lipids. Absolutely no 3-hydroxy fatty acids were found in DNA lipids. However, both C16 and C18 species represent the main fatty acids of whole-cell and DNA-bound lipids. The presence of DNA-bound lipids even under tough treatment of DNA allows to conclude that these lipids represent a special pool among cellular lipids.
Zhdanov
Renat I
RI
Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russian Federation. zrenat@hotmail.com
Shmyrina
Anastasia S
AS
Zarubina
Tatyana V
TV
Mulyukin
Andrey L
AL
El-Registan
Galina I
GI
Haupt
Nicole
N
Kraus
Angelika
A
Lorenz
Wilhelm
W
D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D011549:Pseudomonas / Q000737:chemistry / Q000235:genetics*
D005227:Fatty Acids; D004247:DNA