Application of paramagnetic beads for purifying Bacillus anthracis protective antigen.
Paramagnetic beads coated with Protein G and Tosylactivated-280 dynabeads have been used to purify Bacillus anthracis protective antigen from a liquid culture. The obtained protein was used in the enzyme-linked immunosorbent assay test to detect B. anthracis protective antigen antibodies in human sera collected from immunized individuals. The purification method using paramagnetic beads is very effective. It is fast, easy and may be carried out practically in any laboratory.
Zarzecka
A
A
Military Institute of Hygiene and Epidemiology, Biological Threats Identification and Countering Center, 24-100 Pulawy, Lubelska 2, Poland. azarzecka@man.pulawy.pl
Bartoszcze
M
M
D000818:Animals; D022122:Anthrax Vaccines / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001408:Bacillus anthracis / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods / Q000662:veterinary*
D022122:Anthrax Vaccines; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial