Medicago truncatula transformation using cotyledon explants.
Medicago truncatula has been developed into a model species for legumes. Large numbers of ESTs have been sequenced, and sequencing of the complete gene-rich space of the genotype Jemalong A17 is in progress. By using cotyledons as explants for Agrobacterium infection and direct shoot formation, this protocol allows for rapid production of transgenic plants from A17 and other genotypes. Transgenic plants can be regenerated and established in the greenhouse in only 3-4 mo after Agrobacterium-mediated transformation. Transformation frequency is in the range of 5-12%.
Wright
Elane
E
Forage Improvement Division, The Samuel Roberts Noble Foundation, Ardmore, OK, USA.
Dixon
Richard A
RA
Wang
Zeng-Yu
ZY
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000254:growth & development; D018548:Cotyledon / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology; D018014:Gene Transfer Techniques; D005838:Genotype; D046913:Medicago truncatula / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology; D014170:Transformation, Genetic