Global sperm deoxyribonucleic acid methylation is unaffected in protamine-deficient infertile males.
Sperm protamine-1 (P1) and protamine-2 (P2) concentrations were evaluated concomitantly with global DNA methylation patterns in a population of male infertility patients. Protamine quantification and immunofluorescence microscopy in conjunction with quantitative image analysis revealed no significant relationships between the P1/P2 ratio, P1 concentrations, or P2 concentrations and levels of global DNA methylation in mature spermatozoa.
Aoki
Vincent W
VW
Andrology and IVF Laboratories, University of Utah School of Medicine, Salt Lake City, Utah 84108, USA.
Emery
Benjamin R
BR
Carrell
Douglas T
DT
D000328:Adult; D019175:DNA Methylation; D006801:Humans; D007248:Infertility, Male / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D008297:Male; D011479:Protamines / Q000097:blood*; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology
C082073:PRM1 protein, human; D011479:Protamines; C068621:protamine 2