Isomer-specific effect of conjugated linoleic acid on inflammatory adipokines associated with fat accumulation in 3T3-L1 adipocytes.
The purpose of this study was to examine the isomer-specific effect of conjugated linoleic acid (CLA) on inflammatory markers associated with fat accumulation in cultures of differentiating 3T3-L1 adipocytes. trans-10,cis-12 CLA (t10c12 CLA) reduced leptin secretion and fat accumulation. Linoleic acid (LA) and cis-9,trans-11 CLA (c9t11 CLA) increased them, but not significantly. t10c12 CLA and LA showed similar effects on mRNA expression of inflammatory markers. t10c12 CLA and LA tended to up-regulate the mRNA levels of inflammatory cytokines such as interleukin (IL)-6 (not significantly), tumor necrosis factor (TNF)-alpha, and C-reactive protein (CRP) with no significant change in the secretion of adiponectin, an anti-inflammatory adipokine. However, c9t11 CLA induced no significant change in the mRNA expression of IL-6, TNF-alpha, or CRP, but significantly increased adiponectin secretion. In conclusion, CLA exerted isomer-specific effects on fat accumulation and mRNA expression of inflammatory markers in 3T3-L1 adipocytes. t10c12 CLA up-regulated inflammatory markers in spite of the decreased fat accumulation, and TNF-alpha might be one of the causal factors.
Ahn
In-Sook
IS
School of Life and Food Sciences, Handong Global University, Pohang, Gyeongbuk, Republic of Korea.
Choi
Bong-Hyuk
BH
Ha
Jung-Heun
JH
Byun
Jae-Min
JM
Shin
Hyun-Gil
HG
Park
Kun-Young
KY
Do
Myoung-Sool
MS
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D052242:Adiponectin / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002097:C-Reactive Protein / Q000235:genetics; D016207:Cytokines / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D015850:Interleukin-6 / Q000235:genetics; D020738:Leptin / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D052242:Adiponectin; D016207:Cytokines; D015850:Interleukin-6; D020738:Leptin; D044243:Linoleic Acids, Conjugated; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C503589:cis-9, trans-11-conjugated linoleic acid; C496197:trans-10,cis-12-conjugated linoleic acid; D002097:C-Reactive Protein