Complex karyotype including chromosomal translocation (8;14) (q24;q32) in one case with B-cell prolymphocytic leukemia.
We report a case of a 64-year-old white female patient, who presented with symptomatic anemia (Hgb: 6.8g/dl), thrombocytopenia (platelets: 94,000/mcl) and leukocytosis (WBC: 156,000/mcl). Peripheral blood smear revealed markedly increased white blood cell count with predominance of atypical lymphoid cells of intermediate size, moderately dense chromatin, and prominent large single nucleoli. Bone marrow aspirate smear showed predominance (78%) of atypical lymphoid cells morphologically identical to those seen in the peripheral blood. The bone marrow core biopsy was hypercellular and packed with prominent infiltrate of prolymphocytes. Immunophenotypic analysis revealed a population of monoclonal cells (75% of all -erythroid cells) characterized by CD45+, CD19+, CD20+, CD5+, HLA-DR+, CD10-, CD23+/-, CD38+ and FMC7-. The abnormal cells were restricted to kappa light chain immunoglobulin with low intensity. Cytogenetic study showed an abnormal clone of eight cells with the following karyotype: 45,X,-X,add(8)(p11.2),t(8;14)(q24;q32),add(20)ql3[8]/46,XX[12]. The relative rarity of B-PLL and the heterogeneity of clinical and laboratory parameters make it difficult to define the natural history and prognosis in all cases. The optimal treatment for B-PLL is still unknown and to date there are no reports of chromosomal abnormalities as a prognostic factor. The patient was treated with six cycles of cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP). Complete remission was achieved according to the criteria defined by National Cancer Institute Working Group for CLL.
Crisostomo
Ricardo H
RH
San Juan City Hospital, Hematology-Oncology Section, Department of Medicine, San Juan, Puerto Rico 00936-8344.
Fernandez
Jose A
JA
Caceres
William
W
D000971:Antineoplastic Combined Chemotherapy Protocols; D002869:Chromosome Aberrations; D002883:Chromosomes, Human, Pair 14; D002898:Chromosomes, Human, Pair 8; D003520:Cyclophosphamide; D004317:Doxorubicin; D005260:Female; D006801:Humans; D007621:Karyotyping; D015448:Leukemia, B-Cell / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D015463:Leukemia, Prolymphocytic / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy; D008875:Middle Aged; D011241:Prednisone; D014178:Translocation, Genetic; D014750:Vincristine
D014750:Vincristine; D004317:Doxorubicin; D003520:Cyclophosphamide; D011241:Prednisone