[The effect of the degree of deacetylation of chitosan on the biocompatibility of chitosan membrane with corneal stromal cells].
Some different membranes were prepared by Chitosan with the degree of deacetylation (DD) of 63.7%, 73.7%, 83% and 97% respectively. To study the biocompatibility of Chitosan membrane toward corneal stromal cells, the rabbit cells were cultured on the surface of different DD chitosan membranes. The morphological characteristics, the cell-adhesion, the cell proliferation and the activity of LDH in the medium were investigated. The results of experiment shows that the DD of Chitosan has very significant effect on the biocompatibility of Chitosan membrane toward corneal stromal cells. The more DD of Chitosan, the less injury was made to corneal stromal cells by the chitosan membrane, which is favor of the growing and adhesion of corneal stromal cells. The biocompatibility of the membrane made with low DD Chitosan with corneal stromal cells became worse.
Yao
Ziang
Z
College of Biomengineer, Dalian University, Dalian 116622, China.
Wu
Haige
H
Han
Baoqin
B
Liu
Wanshun
W
D000107:Acetylation; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D003315:Cornea / Q000166:cytology; D008422:Materials Testing; D008567:Membranes, Artificial; D011817:Rabbits; D017154:Stromal Cells / Q000187:drug effects*
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D048271:Chitosan