title: Allele frequency distributions of Apo B VNTR locus in Cukurova, Turkey.
abstract: The highly polymorphic minisatellites contain a variable number of tandemly repeated (VNTR) DNA sequences. They are extremely useful and informative markers to study genetic variation among human populations. We have analysed the allele frequency distribution at the highly polymorphic apolipoprotein B (Apo B) VNTR locus in order to obtain the population data for the Cukurova region in Turkey by using the polymerase chain reaction and polyacrylamide gel electrophoresis. We observed 10 different alleles and 21 genotypes in a sample of 100 unrelated individuals. The allele frequencies ranged from 0.01 to 0.4, with an expected heterozygosity of 0.69 for the Apo B locus. Alleles 37 (frequency = 0.4) and 35 (frequency = 0.17) were the most common in the Cukurova population. There was a significant deviation from the Hardy-Weinberg equilibrium (HWE) for genotype frequencies (chi2 = 29.12; df = 1; p = 0.000). This study possesses novelty as it is the first DNA polymorphism study conducted at the Cukurova population using an Apo B minisatellite locus.
authors.affiliation: Biochemistry Department, Medical Faculty, Cukurova University, Turkey. eyalin@mail.cu.edu.tr
authors.forename: Erdinc
authors.initials: E
authors.lastname: Yalin
authors.forename: Gulen
authors.initials: G
authors.lastname: Attila
authors.forename: Serap
authors.initials: S
authors.lastname: Yalin
authors.forename: Kiymet
authors.initials: K
authors.lastname: Aksoy
mesh_terms: D000328:Adult; D001055:Apolipoproteins B / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D014421:Turkey
chemical_list: D001055:Apolipoproteins B