Typing of bfpA genes of enteropathogenic Escherichia coli isolated in Thailand and Japan by heteroduplex mobility assay.
We developed a rapid genetic approach for screening bfpA variants of enteropathogenic E. coli(EPEC) using a heteroduplex mobility assay (HMA). A total of 204 human EPEC strains were isolated in Thailand and Japan. Of 34 bfpA-positive EPEC strains, bfpA variants were classified into 5 HMA-types. Different HMA-types were found in EPEC of the same serotypes. The results suggest that HMA is a simple and easy method to analyze polymorphism of bfpA gene, and can be used in laboratories without large apparatus such as sequencers.
Iida
Mariko
M
Infectious Diseases Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan. miida@nih.go.jp
Yamazaki
Mitsugu
M
Yatsuyanagi
Jun
J
Ratchtrachenchai
Orn-Anong
OA
Subpasu
Sarayoot
S
Okamura
Noboru
N
Ito
Kenitiro
K
D001483:Base Sequence; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D036781:Fimbriae Proteins / Q000235:genetics*; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D013785:Thailand
C104901:BfpA protein, E coli; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins