title: Human beta-defensin-3 induction in H. pylori-infected gastric mucosal tissues.
abstract: OBJECTIVE
  To examine human beta-defensin-3 (hBD-3) expression in inflamed gastric mucosal tissues or MKN45 gastric cancer cells with or without H. pylori infection for better understanding the innate immune response to H. pylori.
  
  
  METHODS
  We used reverse transcription-polymerase chain reactions and immunohistochemistry to examine hBD-3 expression in inflamed gastric mucosal tissues or MKN45 gastric cancer cells with or without H. pylori. Effects of hBD-3 against H. pylori were also evaluated.
  
  
  RESULTS
  The mean mRNA expression of hBD-3 in H. pylori-positive specimens was significantly higher than that in H pylori-negative specimens (P = 0.0002, Mann-Whitney). In addition, unlike uninfected samples, 8 of 15 (53.33%) infected mucosal samples expressed hBD-3 protein. H. pylori dose-dependently induced mRNA expression of hBD-3 in MKN45 cells, an effect inhibited by adding anti-toll-like receptor (TLR)-4 antibody. HBD-3 protein completely inhibited H. pylori growth.
  
  
  CONCLUSIONS
  Our results suggest that like hBD-2, hBD-3 may be involved in the pathophysiology of H. pylori-induced gastritis.
authors.affiliation: Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kawauchi
authors.forename: A
authors.initials: A
authors.lastname: Yagihashi
authors.forename: N
authors.initials: N
authors.lastname: Tsuji
authors.forename: N
authors.initials: N
authors.lastname: Uehara
authors.forename: D
authors.initials: D
authors.lastname: Furuya
authors.forename: D
authors.initials: D
authors.lastname: Kobayashi
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000494:pharmacology; D045744:Cell Line, Tumor; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000503:physiopathology; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D016481:Helicobacter Infections / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D016480:Helicobacter pylori / Q000187:drug effects / Q000472:pathogenicity; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000276:immunology; D023083:beta-Defensins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000888:Antibodies, Anti-Idiotypic; C422950:DEFB103A protein, human; C106641:DEFB4A protein, human; D012333:RNA, Messenger; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D023083:beta-Defensins