The sarcomeric myosin heavy chain gene family in the dog: analysis of isoform diversity and comparison with other mammalian species.
Sarcomeric myosin heavy chains (MyHC) are the major contractile proteins of cardiac and skeletal muscles and belong to class II MyHC. In this study the sequences of nine sarcomeric MyHC isoforms were obtained by combining assembled contigs of the dog genome draft available in the NCBI database. With this information available the dog becomes the second species, after human, for which the sequences of all members of the sarcomeric MyHC gene family are identified. The newly determined sequences of canine MyHC isoforms were aligned with their orthologs in mammals, forming a set of 38 isoforms, to search for the molecular features that determine the structural and functional specificity of each type of isoform. In this way the structural motifs that allow identification of each isoform and are likely determinants of functional properties were identified in six specific regions (surface loop 1, loop 2, loop 3, converter, MLC binding region, and S2 proximal segment).
Maccatrozzo
Lisa
L
Department of Experimental Veterinary Sciences, University of Padova, 35131 Padua, Italy.
Caliaro
Francesca
F
Toniolo
Luana
L
Patruno
Marco
M
Reggiani
Carlo
C
Mascarello
Francesco
F
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002874:Chromosome Mapping; D004285:Dogs / Q000235:genetics*; D006801:Humans; D008322:Mammals / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D018995:Myosin Heavy Chains / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018994:Myosin Light Chains / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012518:Sarcomeres / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018994:Myosin Light Chains; D020033:Protein Isoforms; D018995:Myosin Heavy Chains