Impact of 20:4n-6 supplementation on the fatty acid composition and hemocyte parameters of the Pacific oyster Crassostrea gigas.
Arachidonic acid (20:4n-6, ArA) and its eicosanoid metabolites have been demonstrated to be implicated in immune functions of vertebrates, fish, and insects. Thus, the aim of this study was to assess the impact of ArA supplementation on the FA composition and hemocyte parameters of oysters Crassostrea gigas. Oyster dietary conditioning consisted of direct addition of ArA solutions at a dose of 0, 0.25, or 0.41 microg ArA per mL of seawater into tanks in the presence or absence of T-Iso algae. Results showed significant incorporation of ArA into gill polar lipids when administered with algae (up to 19.7%) or without algae (up to 12.1%). ArA supplementation led to an increase in hemocyte numbers, phagocytosis, and production of reactive oxygen species by hemocytes from ArA-supplemented oysters. Moreover, the inhibitory effect of Vibrio aestuarianus extracellular products on the adhesive proprieties of hemocytes was lessened in oysters fed ArA-supplemented T-Iso. All changes in oyster hemocyte parameters reported in the present study suggest that ArA and/or eicosanoid metabolites affect oyster hemocyte functions.
Delaporte
Maryse
M
UMR 100 Physiologie and Ecophysiologie des Mollusques Marins, Centre IFREMER de Brest, BP70, 29280 Plouzané, France.
Soudant
Philippe
P
Moal
Jeanne
J
Giudicelli
Emilie
E
Lambert
Christophe
C
Séguineau
Catherine
C
Samain
Jean-François
JF
D000273:Adipose Tissue / Q000737:chemistry / Q000187:drug effects; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D002448:Cell Adhesion; D002470:Cell Survival; D049896:Crassostrea / Q000737:chemistry* / Q000187:drug effects*; D019587:Dietary Supplements; D056890:Eukaryota / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry*; D005880:Gills / Q000737:chemistry; D006434:Hemocytes / Q000737:chemistry / Q000502:physiology*; D010586:Phagocytes / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000097:blood / Q000378:metabolism
D005227:Fatty Acids; D017382:Reactive Oxygen Species; D016718:Arachidonic Acid