Production of galacto-manno-oligosaccharides from guar gum by beta-mannanase from Penicillium oxalicum SO.
Beta-mannanase from Penicillium oxalicum SO efficiently hydrolyzed guar galactomannan to galacto-manno-oligosaccharides. Gel filtration estimated the molecular weight of the beta-mannanase as 35 000 and SDS-PAGE as 29 000. The optimum pH was around 5 while a stable pH was reached in the range of 3-6. Optimum temperature was around 60 degrees C at pH 5, while under 60 degrees C activity was stable. HPLC analysis detected oligosaccharides with degrees of polymerization (DP) of 2 to 7 and 2 to 6 released on hydrolysis of guar and locust bean gums, respectively; about 92% of the released sugars were oligosaccharides. In analysis of the sugar distribution on MALDI-TOF-MS, major products of DP 6 and 7 and DP 5 and 6 were confirmed in hydrolysates of guar gum and locust bean gum, respectively. One of the main oligosaccharides released from guar gum, with DP 7, had a high galactose content (Gal/Man = 0.76) and corresponded to a blockwise galactose-substituted mannan type in galactomannan.
Kurakake
Masahiro
M
Department of Applied Biological Science, Fukuyama University, Sanzou, Gakuenchou 1 banchi, Fukuyama, Hiroshima 729-0292, Japan. kurakake@fubac.fukuyama-u.ac.jp
Sumida
Takuya
T
Masuda
Daisuke
D
Oonishi
Saori
S
Komaki
Toshiaki
T
D002851:Chromatography, High Pressure Liquid; D005685:Galactans / Q000737:chemistry / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism* / Q000031:analogs & derivatives; D006863:Hydrogen-Ion Concentration; D008351:Mannans / Q000737:chemistry / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology*; D053149:Plant Gums / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D044902:beta-Mannosidase / Q000378:metabolism*
D005685:Galactans; D008351:Mannans; D009844:Oligosaccharides; D053149:Plant Gums; C012990:galactomannan; C007894:guar gum; D044902:beta-Mannosidase; D008358:Mannose; D005690:Galactose