title: The influence of cholesterol, progesterone, 4-androstenedione and testosterone on the growth of Treponema denticola ATCC 33520 in batch cultures.
abstract: Previously, we have shown that reference and freshly isolated Treponema denticola cultures are capable of metabolising cholesterol, progesterone, 4-androstenedione and testosterone by means of 5alpha-reductase, 3beta-and 17beta-hydroxysteroid dehydrogenase activity [Clark DT, Soory M. The metabolism of cholesterol and certain hormonal steroids by Treponema denticola. Steroids. 2006;71:352-63. ]. The aim of the work presented in this paper was to investigate the modulation of T. denticola growth in batch cultures by these steroids, using T. denticola ATCC 33520 as a model system. Growth curves were summarised using statistics based on optical density and protein yield. Cholesterol was found to stimulate growth at concentrations of 10 and 25microg/mL. Certain hormonal steroids inhibited the maximum achievable optical density at concentrations of 1 and 10microg/mL while the minimum concentration shown to inhibit protein yield was 0.001microg/mL of progesterone. The potential of the hormonal steroids to inhibit growth was in the order of progesterone, 4-androstenedione and testosterone.
authors.affiliation: Department of Microbiology, King's College London Dental Institute at Guy's, King's College and St Thomas' Hospitals, Floor 17 Guy's Tower, London SE1 9RS, UK. douglas.2.clark@kcl.ac.uk
authors.forename: Douglas T
authors.initials: DT
authors.lastname: Clark
authors.forename: Mena
authors.initials: M
authors.lastname: Soory
mesh_terms: D002784:Cholesterol / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D006801:Humans; D010510:Periodontal Diseases / Q000382:microbiology; D048373:Treponema denticola / Q000187:drug effects* / Q000254:growth & development
chemical_list: D003470:Culture Media; D012739:Gonadal Steroid Hormones; D002784:Cholesterol