[Pharmacokinetics of hylotelephin in Beagle dogs].
OBJECTIVE
To investigate the pharmacokinetics of hylotelephin in Beagle dogs and obtain the main pharmacokinetic parameters.


METHODS
An HPLC method with UV detection was developed to study the pharmacokinetics of hylotelephin in dogs by joining an internal standard (anthracene). Benzoyl chloride was used to the pre-column derivatization of hylotelephin and methanol-water (64:36) was used as the mobile phase. According to the 3P97 pharmacokinetic program, the main parameters were calculated.


RESULTS
The hylotelephin pharmacokinetics conforms to a two-compartment open model after a single iv dose of hylotelephin 10.6 or 21.3 mg x kg(-1) in Beagle dogs. The parameters of two groups were as follows: T(1/2) alpha were 2.3 and 2.1 min, T(1/2) beta were 1.9 and 2.0 h, K12 were 0. 12 and 0.11 min, K21 were 0.17 and 0.21 min, K10 were 0.011 and 0.0094 min, Vc were 0.54 and 0.54 L x kg(-1), AUC were 1.8 and 4.1 g x min x L(-1), CL were 0.0048 and 0.0056 L x kg(-1) x min(-1), MRT were 2.10 and 2.4 h, respectively.


CONCLUSIONS
The pharmacokinetics of hylotelephin after iv administration showed a rapid distribution and elimination process in Beagle dogs and was of first order kinetics.
Liu
Ying-ju
YJ
Department of Pharmacology, Chongqing University of Medical Sciences, Chongqing 400016, China. liuyingjbh@yahoo.com.cn
Shang
Jing-chuan
JC
Gao
Li-jia
LJ
He
Ying-na
YN
Zhou
Li-ping
LP
Mu
Zhao-de
ZD
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics*; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid; D029627:Crassulaceae / Q000737:chemistry; D004285:Dogs; D005260:Female; D006575:Heterocyclic Compounds, 3-Ring / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics*; D008297:Male; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000998:Antiviral Agents; D006575:Heterocyclic Compounds, 3-Ring; C526384:hylotelephin