title: Protein composition of the vaccinia virus mature virion.
abstract: The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugations and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass spectrometry to detect tryptic peptides separated by high-resolution liquid chromatography. Eighty vaccinia virus-encoded proteins representing 37% of the 218 genes annotated in the complete genome sequence were detected in at least three analyses. Ten proteins accounted for approximately 80% of the virion mass. Thirteen identified proteins were not previously reported as components of virions. On the other hand, 8 previously described virion proteins were not detected here, presumably due to technical reasons including small size and hydrophobicity. In addition to vaccinia virus-encoded proteins, 24 host proteins omitting isoforms were detected. The most abundant of these were cytoskeletal proteins, heat shock proteins and proteins involved in translation.
authors.affiliation: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4 Center Drive, MSC 0445, Bethesda, MD 20892-0445, USA.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Resch
authors.forename: Kim K
authors.initials: KK
authors.lastname: Hixson
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Moore
authors.forename: Mary S
authors.initials: MS
authors.lastname: Lipton
authors.forename: Bernard
authors.initials: B
authors.lastname: Moss
mesh_terms: D002500:Centrifugation, Isopycnic; D002853:Chromatography, Liquid; D017550:Spectroscopy, Fourier Transform Infrared; D053719:Tandem Mass Spectrometry; D014616:Vaccinia virus / Q000737:chemistry* / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis*; D014771:Virion / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D014764:Viral Proteins