In vitro growth and maturation of caprine oocytes.
This study was conducted to culture in vitro caprine pre-antral follicles for determining the competence of growth and maturation of oocytes and establishing a suitable culture system for oocyte maturation from pre-antral follicles. Two different culture methods (microdrop and agar gel clot) were employed to culture caprine pre-antral follicles. The pre-antral follicles were isolated from prepubertal goat ovaries by treatment with collagenase and DNase. The isolated pre-antral follicles were cultured in basic culture medium for 9 days (for growth). And oocytes were cultured in maturation culture medium for another 2 days for maturation. The result demonstrated that the growth rate of oocytes cultured in microdrops was significantly (p < 0.05) higher than that in agar gel clots, whereas the viability of oocytes in microdrops was considerably (p < 0.05) lower than that in agar gel clots. The oocytes grew over 150 microm in diameter, and two of 151 oocytes cultured in microdrops yielded morphologically abnormal first polar bodies. However, the size of oocytes cultured in agar gel approached to 120 microm in diameter and no polar body was produced.
Zhou
H M
HM
Collage of Bioengineering, Inner Mongolia Agricultural University, Hohhot, China. huanminzhou@263.net
Zhang
Y
Y
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006041:Goats / Q000502:physiology*; D009865:Oocytes / Q000254:growth & development* / Q000502:physiology*; D046509:Tissue Culture Techniques / Q000662:veterinary*
D003470:Culture Media