Optimization of nutritional requirements and feeding strategies for clavulanic acid production by Streptomyces clavuligerus.
The present work reports the nutritional requirements and environmental conditions for submerged culture of Streptomyces clavuligerus for clavulanic acid production using orthogonal matrix method (Taguchi L(16) design) and also fed-batch fermentation for clavulanic acid production by feeding glycerol, arginine and threonoine to the fermentation medium intermittently. Clavulanic acid production was increased by 18% with the span of feeding glycerol and reached a maximum at 1.30mg/ml with 120h glycerol feeding as compared to 1.10mg/ml in the control. The production also increased with the span of feeding amino acids and reached a maximum of 1.31 and 1.86mg/ml with feeding arginine and threonine, respectively in 120h. There was an overall increase of 18% and 9% in clavulanic acid production with arginine and threonine feeding as compared to the respective controls (1.10 and 1.70mg/ml, respectively).
Saudagar
Parag S
PS
Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400 019, India.
Singhal
Rekha S
RS
D001120:Arginine / Q000378:metabolism; D001431:Bacteriological Techniques; D019818:Clavulanic Acid / Q000096:biosynthesis* / Q000737:chemistry; D003470:Culture Media; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism*; D013912:Threonine / Q000378:metabolism
D003470:Culture Media; D010710:Phosphates; D019818:Clavulanic Acid; D013912:Threonine; D001120:Arginine; D005990:Glycerol