title: Characterisation of the main clones of Streptococcus pyogenes carrying the ermA (subclass TR) gene in Spain.
abstract: Seventy-four Streptococcus pyogenes isolates showing the macrolide-lincosamide-streptogramin B (MLS(B)) resistance phenotype carrying the ermA gene (72 of which showed the inducible resistance phenotype) were obtained between 1999 and 2004. Seven different sequence types (STs) and emm types were detected: emm22/ST46 (n=33); emm77/ST63 (n=22); emm73/ST331 (n=10); emm94/ST89 (n=6); and one isolate each of emm28/ST52, emm11/ST403 and emm4/ST38. All ST46 isolates were susceptible to tetracycline and almost all reacted against the T12 type (all agglutinated into the T-pattern 3/12/13/B3264). Resistance to tetracycline was observed in all ST63 (tetO+) and ST89 (tetM+) isolates. Most of the ST63 isolates reacted against the T28 type (all agglutinated into the T-pattern 9/13/28). The 74 isolates were grouped into eight pulsed-field gel electrophoresis pulsotypes (one cluster for each emm/ST type, except for emm77/ST63).
authors.affiliation: Servicio de Microbiología, Hospital Donostia, Paseo Dr. Beguiristain s/n, 20014 San Sebastián, Spain.
authors.forename: Milagrosa
authors.initials: M
authors.lastname: Montes
authors.forename: Beatriz
authors.initials: B
authors.lastname: Orden
authors.forename: Esther
authors.initials: E
authors.lastname: Tamayo
authors.forename: Juan-Ignacio
authors.initials: JI
authors.lastname: Alos
authors.forename: Emilio
authors.initials: E
authors.lastname: Pérez-Trallero
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004917:Erythromycin / Q000494:pharmacology; D006801:Humans; D008780:Methyltransferases / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D013030:Spain; D013297:Streptococcus pyogenes / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D013753:Tetracycline Resistance
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; C083138:Tet M resistance protein, Bacteria; C083139:Tet O resistance protein, Bacteria; D004917:Erythromycin; D008780:Methyltransferases; C431709:ErmA protein, Bacteria