Maize (Zea mays L.).
Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the greenhouse.
Frame
Bronwyn R
BR
Center for Plant Transformation, Plant Science Institute, Iowa State University, Ames, IA, USA.
Paque
Tina
T
Wang
Kan
K
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000254:growth & development; D018920:Coculture Techniques; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology; D014170:Transformation, Genetic; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology