title: Carcinoembryonic antigen (CEA) mimicry by an anti-idiotypic scFv isolated from anti-Id 6.C4 hybridoma.
abstract: Since carcinoembryonic antigen (CEA) is expressed during embryonic life, it is not immunogenic in humans. The use of anti-idiotypic (Id) antibodies as a surrogate of antigen in the immunization has been considered a promising strategy for breaking tolerance to some tumor associated antigens. We have described an anti-Id monoclonal antibody (MAb), designated 6.C4, which is able to mimic CEA functionally. The anti-Id MAb 6.C4 was shown to elicit antibodies that recognized CEA in vitro and in vivo. In the present study, we sought to verify whether a single chain (scFv) antibody obtained, the scFv 6.C4, would retain the ability to mimic CEA. Two scFv containing the variable heavy and light chain domains of 6.C4 were constructed with a 15-amino acid linker: one with and another without signal peptide. DNA immunization of mice with both forms of scFv individually elicited antibodies able to recognize CEA.
authors.affiliation: Biophysics Department, Federal University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Graciela Conceição
authors.initials: GC
authors.lastname: Pignatari
authors.forename: Daniela
authors.initials: D
authors.lastname: Takeshita
authors.forename: Carolina Bellini
authors.initials: CB
authors.lastname: Parise
authors.forename: Fernando Augusto
authors.initials: FA
authors.lastname: Soares
authors.forename: Jane Zveiter
authors.initials: JZ
authors.lastname: de Moraes
authors.forename: Sang Won
authors.initials: SW
authors.lastname: Han
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D000918:Antibody Specificity; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D003110:Colonic Neoplasms / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007128:Immunoglobulin Fragments / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D002272:Carcinoembryonic Antigen; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D019444:Vaccines, DNA