title: Relationship of androgens to insulin resistance and chronic inflammation in morbidly obese premenopausal women: studies before and after vertical banded gastroplasty.
abstract: BACKGROUND
  Morbid obesity is associated with insulin resistance (IR), type 2 diabetes, lipid abnormalities, and hypertension. The association of obesity with increased androgen production and low concentrations of sex hormone-binding globulin (SHBG) in women has been demonstrated as well as a strong association of androgens with markers of inflammation such as high-sensitive C-reactive protein (hsCRP). Because weight loss results in a significant decrease in cardiovascular risk factors, IR and inflammation, we questioned a possible interrelationship between androgens, IR and inflammation in a prospective study with 43 morbidly obese female patients undergoing bariatric surgery.
  
  
  METHODS
  SHBG, dehydroepiandrosterone-sulfate (DHEA-S), and insulin were measured by ELISA, cortisol by fluorescence polarization immunoassay, androstendione by RIA, and testosterone by electrochemiluminescence immunoassay. The free androgen index (FAI) was calculated as the total testosterone/SHBG ratio. High sensitivity assays were used to obtain concentrations of fasting hsCRP, Interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha).
  
  
  RESULTS
  Weight loss resulted in a significant increase in SHBG (17+/-12 vs 70+/-30 nmol/l, P<0.0001). Serum concentrations of androstendione, total testosterone, DHEA-S and the FAI decreased significantly (2.05+/-0.78 vs 1.40+/-0.54 ng/ml, P<0.0001; 1.88+/-0.84 vs 1.16+/-0.57 nmol/L, P<0.0001; 1.72+/-0.86 vs 1.49+/-0.78 microg/ml, P<0.01; 0.15+/-0.10 vs 0.02+/-0.01, P<0.0001; respectively). Preoperatively and postoperatively, no association between androgens and IR, chronic subclinical inflammation and lipid parameters could be observed.
  
  
  CONCLUSIONS
  Massive weight loss induced by bariatric surgery is associated with a significant reduction of androgen concentrations but not related to the concomitant decline of chronic inflammation, IR, glucose and lipid abnormalities in premenopausal morbidly obese women.
authors.affiliation: Department of Medicine I, Rudolfstiftung Hospital, Vienna, Austria. Hans-Peter.Kopp@wienkav.at
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Kopp
authors.forename: Katharina
authors.initials: K
authors.lastname: Krzyzanowska
authors.forename: Gerit-Holger
authors.initials: GH
authors.lastname: Schernthaner
authors.forename: Stefan
authors.initials: S
authors.lastname: Kriwanek
authors.forename: Guntram
authors.initials: G
authors.lastname: Schernthaner
mesh_terms: D000328:Adult; D002097:C-Reactive Protein / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D015391:Gastroplasty; D006801:Humans; D007333:Insulin Resistance / Q000502:physiology*; D015850:Interleukin-6 / Q000097:blood; D008875:Middle Aged; D009767:Obesity, Morbid / Q000097:blood* / Q000601:surgery*; D017697:Premenopause / Q000097:blood; D011446:Prospective Studies; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism; D045165:Testosterone Congeners / Q000097:blood*; D015431:Weight Loss / Q000502:physiology
chemical_list: D015850:Interleukin-6; D012738:Sex Hormone-Binding Globulin; D045165:Testosterone Congeners; D002097:C-Reactive Protein