Fast differentiation of meats from fifteen animal species by liquid chromatography with electrochemical detection using copper nanoparticle plated electrodes.
A simple, rapid and reliable method based on high-performance liquid chromatography with electrochemical detection was developed to routinely differentiate among meat products from fifteen food animal species. Samples from cattle, pigs, goats, deer, horses, chickens, ducks, ostriches, salmon, cod, shrimp, crabs, scallops, bullfrogs and alligators each exhibited unique electrochemical profiles. Species-specific markers exhibited reproducible peak retention times with coefficients of variation less then 6% across different runs, body regions and subjects. The method requires no derivatization or extraction steps and may be applicable to fresh or cooked meats. Incubation of fresh beef, pork or chicken at room temperature for 24h or repeated freezing and thawing changed the intensity but not the pattern of species-specific peaks. In conclusion, this method appears suitable for rapid differentiation of meats from various food animal species and demonstrates the utility of electrochemical detection to supplement existing immunochemical and molecular biological methods. The possibility of using this method to detect adulteration and degradative changes of meat proteins is discussed.
Chou
Chi-Chung
CC
Department of Veterinary Medicine and Veterinary Medicine Teaching Hospital, College of Veterinary Medicine, National Chung-Hsing University, Taichung 402, Taiwan. ccchou@nchu.edu.tw
Lin
Show-Ping
SP
Lee
Kuo-Ming
KM
Hsu
Cheng-Teng
CT
Vickroy
Thomas W
TW
Zen
Jyh-Myng
JM
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D008460:Meat; D053768:Metal Nanoparticles; D013045:Species Specificity
D003300:Copper