Role of the ptsN gene product in catabolite repression of the Pseudomonas putida TOL toluene degradation pathway in chemostat cultures.
The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIA(Ntr) participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influence expression when cells were growing in a chemostat with succinate at maximum rate.
Aranda-Olmedo
Isabel
I
Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Dept. of Environmental Protection, Granada E-18008, Spain.
Marín
Patricia
P
Ramos
Juan L
JL
Marqués
Silvia
S
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism*; D016958:Pseudomonas putida / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism; D014050:Toluene / Q000378:metabolism*
D001426:Bacterial Proteins; D003470:Culture Media; C423631:PtsN protein, Pseudomonas putida; D012097:Repressor Proteins; D013386:Succinates; C101896:crc protein, Pseudomonas; D014050:Toluene; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System