title: Genetic characterization of the phenylacetyl-coenzyme A oxygenase from the aerobic phenylacetic acid degradation pathway of Escherichia coli.
abstract: We show here that the paaABCDE genes of the paa cluster responsible for phenylacetate degradation in Escherichia coli W encode a five-component oxygenase that hydroxylates phenylacetyl-coenzyme A (CoA), the first intermediate of the pathway. The primary structure of the subunits of bacterial phenylacetyl-CoA oxygenases revealed that these enzymes constitute the prototype of a new and distinct group of the large bacterial diiron multicomponent oxygenase family.
authors.affiliation: Department of Molecular Microbiology, Centro de Invetigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Fernández
authors.forename: Abel
authors.initials: A
authors.lastname: Ferrández
authors.forename: Baltasar
authors.initials: B
authors.lastname: Miñambres
authors.forename: Eduardo
authors.initials: E
authors.lastname: Díaz
authors.forename: José L
authors.initials: JL
authors.lastname: García
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000332:Aerobiosis; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D019143:Evolution, Molecular; D010648:Phenylacetates / Q000378:metabolism; D010802:Phylogeny; D010957:Plasmids
chemical_list: D010648:Phenylacetates; D000105:Acetyl Coenzyme A; C037816:phenylacetyl-coenzyme A; C025136:phenylacetic acid