Electrochemical impedance spectroscopy for study of amyloid beta-peptide interactions with (-) nicotine ditartrate and (-) cotinine.
Immobilization of amyloid beta (Abeta) (1-40) peptide on Au-colloid modified gold electrodes has been studied. Colloidal Au was self-assembled onto gold electrodes through the thiol groups of 1,6-hexanedithiol monolayer. Next, buffered aqueous solution of Abeta (1-40) peptide existing in the beta-sheet structure in the acidic media was dropped on the electrode surface. Each step of electrode modification has been confirmed with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The changes of the resistance of the layer with deposited Abeta (1-40) peptide, occurred under stimulation by different concentration of (-) nicotine ditartrate and (-) cotinine were measured with EIS and were used for the calculation of association constants. The gentle measuring conditions applied in electrochemical impedance spectroscopy, together with suitable environment for biomolecules immobilization created by Au-colloid, might be recommended as the analytical tool for assessing the effectiveness of potential drugs used in Alzheimer's disease (AD) therapy.
Szymańska
Iwona
I
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Division of Food Sciences, Tuwima 10, 10-747 Olsztyn, Poland.
Radecka
Hanna
H
Radecki
Jerzy
J
Kaliszan
Roman
R
D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism*; D003367:Cotinine / Q000737:chemistry / Q000378:metabolism*; D017097:Electric Impedance; D004566:Electrodes; D017979:Gold Colloid; D009538:Nicotine / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D013057:Spectrum Analysis
D016229:Amyloid beta-Peptides; D017979:Gold Colloid; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); D009538:Nicotine; D003367:Cotinine