Change in decorin during aging of rat placenta.
By immunohistochemistry, with or without chondroitinases, decorin was found to be distributed in the extracellular matrix of chorionic villi and amnia. The strength of staining intensified with increasing gestational age. Decorin was isolated from the placenta of 13- to 20-day-old pregnant rats and identified by Western blotting, using an antidecorin core protein antibody. The molecular weight of decorin is approximately 100 kDa, whereas the respective figures for the core protein treated with chondroitinase (chase) ABC and with chase B are approximately 40 kDa and 43 kDa. The difference in the molecular weight between the core protein with chase ABC and B suggests that the glycosaminoglycan (GAG)- base structure on the core protein was chondroitin sulfate (CS) without dermatan sulfate (DS). The decorin content and the proportion of CS to DS in GAG increased with age. We concluded that the age-related changes in the GAG chain may be related to specific functional properties and may have a crucial role in placental tissue organization.
Batbayal
Tumurbaatar
T
Department of Applied Protein Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
Ishii
Yasuhiro
Y
Nomura
Yoshihiro
Y
Watanabe
Mutsuto
M
Yasuko
Toi
T
Nakamura
Shinji
S
D000367:Age Factors; D000375:Aging / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D058575:Decorin; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005865:Gestational Age; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D010920:Placenta / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D011509:Proteoglycans / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
C546880:Dcn protein, rat; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans