SNPs in human beta-defensin 1 gene (DEFB1): frequencies in a Mexican population and new PCR-RFLPs assays.
Polymerase chain reaction-restriction fragment length polymorphism assays for two single nucleotide polymorphisms in the human beta-defensin 1 gene have been validated with real-time PCR in 101 healthy individuals from western Mexico. Allele frequencies were 52.5% (692-A) and 98.5% (1836-A). These assays can be confidently used as a cheaper alternative genotyping method for these sites.
Prado-Montes de Oca
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PhD Program in Human Genetics, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara, Jalisco, México.
Rangel-Villalobos
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Gallegos-Arreola
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MP
Sandoval
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Figuera
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D005006:Ethnicity / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008800:Mexico; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D023083:beta-Defensins / Q000235:genetics*
C406407:DEFB1 protein, human; D023083:beta-Defensins