Human beta-defensin-3 induction in H. pylori-infected gastric mucosal tissues.
OBJECTIVE
To examine human beta-defensin-3 (hBD-3) expression in inflamed gastric mucosal tissues or MKN45 gastric cancer cells with or without H. pylori infection for better understanding the innate immune response to H. pylori.


METHODS
We used reverse transcription-polymerase chain reactions and immunohistochemistry to examine hBD-3 expression in inflamed gastric mucosal tissues or MKN45 gastric cancer cells with or without H. pylori. Effects of hBD-3 against H. pylori were also evaluated.


RESULTS
The mean mRNA expression of hBD-3 in H. pylori-positive specimens was significantly higher than that in H pylori-negative specimens (P = 0.0002, Mann-Whitney). In addition, unlike uninfected samples, 8 of 15 (53.33%) infected mucosal samples expressed hBD-3 protein. H. pylori dose-dependently induced mRNA expression of hBD-3 in MKN45 cells, an effect inhibited by adding anti-toll-like receptor (TLR)-4 antibody. HBD-3 protein completely inhibited H. pylori growth.


CONCLUSIONS
Our results suggest that like hBD-2, hBD-3 may be involved in the pathophysiology of H. pylori-induced gastritis.
Kawauchi
K
K
Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan.
Yagihashi
A
A
Tsuji
N
N
Uehara
N
N
Furuya
D
D
Kobayashi
D
D
Watanabe
N
N
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000494:pharmacology; D045744:Cell Line, Tumor; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000503:physiopathology; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D016481:Helicobacter Infections / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D016480:Helicobacter pylori / Q000187:drug effects / Q000472:pathogenicity; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000276:immunology; D023083:beta-Defensins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; C422950:DEFB103A protein, human; C106641:DEFB4A protein, human; D012333:RNA, Messenger; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D023083:beta-Defensins