title: Simultaneous production of sphingolipids and ethanol by Kluyveromyces thermotolerans.
abstract: Kluyveromyces thermotolerans strain NBRC 1674 was selected for the simultaneous production of sphingolipids and ethanol from beet molasses. The strain gradually synthesized ethanol with fermentation periods and attained a level slightly higher than that of the strain of Saccharomyces cerevisiae usually used for ethanol production. The sphingolipids accumulated in the cells were composed of almost equal amounts of free ceramides and glucosylceramides. The sphingoid bases and fatty acids of the two sphingolipids differed from each other and changed under aerobic and anaerobic growth conditions. Oxygen limitation may cause accumulation of sphinganine by inhibiting sphingolipid desaturases and enhance its proportion in both the sphingolipids.
authors.affiliation: Shimizu Bio-Plants, Nippon Beet Sugar Manufacturing Co. Ltd., Shimizu, Kamikawa, Hokkaido 089-0103, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tamura
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Yunoki
authors.forename: O
authors.initials: O
authors.lastname: Matsumoto
authors.forename: N
authors.initials: N
authors.lastname: Takakuwa
authors.forename: Y
authors.initials: Y
authors.lastname: Oda
authors.forename: M
authors.initials: M
authors.lastname: Ohnishi
mesh_terms: D000693:Anaerobiosis / Q000502:physiology; D002518:Ceramides / Q000032:analysis / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D007716:Kluyveromyces / Q000378:metabolism*; D013107:Sphingolipids / Q000378:metabolism*
chemical_list: D002518:Ceramides; D013107:Sphingolipids; D000431:Ethanol