Generation and immunogenicity of a recombinant pseudorabies virus expressing cap protein of porcine circovirus type 2.
Porcine circovirus type 2 (PCV2) is associated with post-weaning multisystemic wasting syndrome (PMWS), which is an important economical disease affecting the pig industry worldwide. In order to develop an effective vaccine for PMWS, a recombinant pseudorabies virus (PRV) was generated and tested in piglets in this study. The PCV2 open reading frame 2 (ORF2) gene was inserted into pIECMV plasmid and co-transfected with PRV Tk-/gE-/LacZ+ genome into IBRS-2 cells to generate a recombinant Tk-/gE-/ORF2(+) virus. The expression of PCV2 ORF2 gene in the recombinant virus was confirmed by Western blotting and indirect immunofluorescence assay (IFA). Four-week-old piglets were immunized by the recombinant virus, and the immunogenicity of PRV Tk-/gE-/ORF2(+) was tested by PRV-enzyme-linked immunosorbent assay (ELISA), PRV neutralizing assay, ORF2-ELISA and ORF2 specific lymphocyte proliferation response. PRV Tk-/gE-/ORF2(+) elicited significant humoral immune responses to both PRV and PCV2, and the PCV2-specific lymphocyte proliferation response could be detected on day 49 of this experiment. These findings suggest that the recombinant PRV Tk-/gE-/ORF2(+) may be a potential vaccine against both PCV2 and PRV.
Song
Yunfeng
Y
Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
Jin
Meilin
M
Zhang
Songlin
S
Xu
Xiaojuan
X
Xiao
Shaobo
S
Cao
Shengbo
S
Chen
Huanchun
H
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D018173:Circoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D005822:Genetic Vectors; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology; D007114:Immunization / Q000662:veterinary; D008213:Lymphocyte Activation; D009500:Neutralization Tests / Q000662:veterinary; D016366:Open Reading Frames; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology*; D014614:Vaccines, Synthetic; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology; D019282:Wasting Syndrome / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology
D036022:Capsid Proteins; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines