title: Autofluorescence endoscopy in surveillance of Barrett's esophagus: a multicenter randomized trial on diagnostic efficacy.
abstract: OBJECTIVE
  The reference surveillance method in patients with Barrett's esophagus is careful endoscopic observation, with targeted as well as random four-quadrant biopsies. Autofluorescence endoscopy (AFE) may make it easier to locate neoplasia. The aim of this study was to elucidate the diagnostic accuracy of surveillance with AFE-guided plus four-quadrant biopsies in comparison with the conventional approach.
  
  
  METHODS
  A total of 187 of 200 consecutive Barrett's esophagus patients who were initially enrolled (73 % male, mean age 67 years, mean Barrett's segment length 4.6 cm), who underwent endoscopy for Barrett's esophagus in four study centers, were randomly assigned to undergo either AFE-targeted biopsy followed by four-quadrant biopsies or conventional endoscopic surveillance, also including four-quadrant biopsies (study phase 1). After exclusion of patients with early cancer or high-grade dysplasia, who underwent endoscopic or surgical treatment, as well as those who declined to participate in phase 2 of the study, 130 patients remained. These patients were examined again with the alternative method after a mean of 10 weeks, using the same methods described. The main study parameter was the detection of early cancer/adenocarcinoma or high-grade dysplasia (HGD), comparing both approaches in study phase 1; the secondary study aim in phase 2 was to assess the additional value of the AFE-guided approach after conventional surveillance, and vice versa. Test accuracy measures were derived from study phase 1.
  
  
  RESULTS
  In study phase 1, the AFE and conventional approaches yielded adenocarcinoma/HGD rates of 12 % and 5.3 %, respectively, on a per-patient basis. With AFE, four previously unrecognized adenocarcinoma/HGD lesions were identified (4.3 % of the patients); with the conventional approach, one new lesion (1.1 %) was identified. Of the 19 adenocarcinoma/HGD lesions detected during AFE endoscopy in study phase 1, eight were visualized, while 11 were only detected using untargeted four-quadrant biopsies (sensitivity 42 %). Of the 766 biopsies classified at histology as being nonneoplastic, 58 appeared suspicious (specificity 92 %, positive predictive value 12 %, negative predictive value 98.5 %). In study phase 2, AFE detected two further lesions in addition to the initial alternative approach in 3.2 % of cases, in comparison with one lesion with conventional endoscopy (1.7 %).
  
  
  CONCLUSIONS
  In this referral Barrett's esophagus population with a higher prevalence of neoplastic lesions, the AFE-guided approach improved the diagnostic yield for neoplasia in comparison with the conventional approach using four-quadrant biopsies. However, AFE alone was not suitable for replacing the standard four-quadrant biopsy protocol.
authors.affiliation: Division of Gastroenterology/Hepatology, Dept. of Internal Medicine, Cantonal Hospital, St. Gallen, Switzerland. jan.borovicka@kssg.ch
authors.forename: J
authors.initials: J
authors.lastname: Borovicka
authors.forename: J
authors.initials: J
authors.lastname: Fischer
authors.forename: J
authors.initials: J
authors.lastname: Neuweiler
authors.forename: P
authors.initials: P
authors.lastname: Netzer
authors.forename: J
authors.initials: J
authors.lastname: Gschossmann
authors.forename: T
authors.initials: T
authors.lastname: Ehmann
authors.forename: P
authors.initials: P
authors.lastname: Bauerfeind
authors.forename: G
authors.initials: G
authors.lastname: Dorta
authors.forename: U
authors.initials: U
authors.lastname: Zürcher
authors.forename: J
authors.initials: J
authors.lastname: Binek
authors.forename: C
authors.initials: C
authors.lastname: Meyenberger
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis*; D000368:Aged; D001471:Barrett Esophagus / Q000175:diagnosis*; D001706:Biopsy / Q000379:methods; D016099:Endoscopy, Gastrointestinal / Q000379:methods*; D004938:Esophageal Neoplasms / Q000175:diagnosis*; D005453:Fluorescence; D006801:Humans; D008875:Middle Aged