title: Isolation and characterization of the potential receptor for wheat germ agglutinin from human neutrophils.
abstract: Neutrophils participate in host protection and central to this process is the regulation of oxidative mechanisms. We purified by affinity chromatography the receptor for the GlcNAc-specific WGA from CD14+ CD16+ cell lysates (WGAr). The receptor is a 141 kDa glycoprotein constituted by two subunits of 78 and 63 kDa. It is mainly composed of Ser, Asx, and Gly, and, in a minor proportion, His, Cys, and Pro. Its glycan portion contains GlcNAc, Gal, and Man; NeuAc and GalNAc were identified in a minor proportion. The amino acid sequence of the WGA receptor was predicted from tryptic peptides by MALDI-TOF, both subunits showed homology with cytokeratin type II (26 and 29% for the 78 and 63 kDa subunits, respectively); the 78 kDa subunit showed also homology with the human transferrin receptor (24%). Antibodies against WGAr induce higher oxidative burst than WGA, determined by NBT reduction; however, this effect was inhibited (p < 0.05) with GlcNAc suggesting that WGAr participates as mediator in signal transduction in neutrophils.
authors.affiliation: Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, Tlalpan, 14080, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Solórzano
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bouquelet
authors.forename: M Ali
authors.initials: MA
authors.lastname: Pereyra
authors.forename: Francisco
authors.initials: F
authors.lastname: Blanco-Favela
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Slomianny
authors.forename: Raúl
authors.initials: R
authors.lastname: Chavez
authors.forename: Ricardo
authors.initials: R
authors.lastname: Lascurain
authors.forename: Edgar
authors.initials: E
authors.lastname: Zenteno
authors.forename: Concepción
authors.initials: C
authors.lastname: Agundis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D010449:Peptide Mapping; D021122:Protein Subunits; D011975:Receptors, Mitogen / Q000097:blood* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D016897:Respiratory Burst; D014909:Wheat Germ Agglutinins / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D021122:Protein Subunits; D011975:Receptors, Mitogen; D014909:Wheat Germ Agglutinins; C027503:wheat germ agglutinin receptor