title: Minimizing the risk of reporting false positives in large-scale RNAi screens.
abstract: Large-scale RNA interference (RNAi)-based analyses, very much as other 'omic' approaches, have inherent rates of false positives and negatives. The variability in the standards of care applied to validate results from these studies, if left unchecked, could eventually begin to undermine the credibility of RNAi as a powerful functional approach. This Commentary is an invitation to an open discussion started among various users of RNAi to set forth accepted standards that would insure the quality and accuracy of information in the large datasets coming out of genome-scale screens.
authors.affiliation: Cenix BioScience GmbH, Tatzberg 47, Dresden, 10307, Germany. echeverri@cenix-bioscience.com
authors.forename: Christophe J
authors.initials: CJ
authors.lastname: Echeverri
authors.forename: Philip A
authors.initials: PA
authors.lastname: Beachy
authors.forename: Buzz
authors.initials: B
authors.lastname: Baum
authors.forename: Michael
authors.initials: M
authors.lastname: Boutros
authors.forename: Frank
authors.initials: F
authors.lastname: Buchholz
authors.forename: Sumit K
authors.initials: SK
authors.lastname: Chanda
authors.forename: Julian
authors.initials: J
authors.lastname: Downward
authors.forename: Jan
authors.initials: J
authors.lastname: Ellenberg
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Fraser
authors.forename: Nir
authors.initials: N
authors.lastname: Hacohen
authors.forename: William C
authors.initials: WC
authors.lastname: Hahn
authors.forename: Aimee L
authors.initials: AL
authors.lastname: Jackson
authors.forename: Amy
authors.initials: A
authors.lastname: Kiger
authors.forename: Peter S
authors.initials: PS
authors.lastname: Linsley
authors.forename: Lawrence
authors.initials: L
authors.lastname: Lum
authors.forename: Yong
authors.initials: Y
authors.lastname: Ma
authors.forename: Bernard
authors.initials: B
authors.lastname: Mathey-Prévôt
authors.forename: David E
authors.initials: DE
authors.lastname: Root
authors.forename: David M
authors.initials: DM
authors.lastname: Sabatini
authors.forename: Jussi
authors.initials: J
authors.lastname: Taipale
authors.forename: Norbert
authors.initials: N
authors.lastname: Perrimon
authors.forename: René
authors.initials: R
authors.lastname: Bernards
mesh_terms: D030541:Databases, Genetic / Q000592:standards; D005189:False Positive Reactions; D005820:Genetic Testing / Q000592:standards*; D010641:Phenotype; D034622:RNA Interference; D012306:Risk; D012680:Sensitivity and Specificity