Ratiometric fluorescence detection of a tag fused protein using the dual-emission artificial molecular probe.
We have successfully developed a ratiometric detection system for protein of interest using the complementary recognition pair of the tetra-aspartate peptide tag and the SNARF-appended Zn(ii)-DpaTyr probe.
Honda
Kei
K
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, 615-8510, Japan.
Nakata
Eiji
E
Ojida
Akio
A
Hamachi
Itaru
I
D000595:Amino Acid Sequence; D001578:Benzopyrans / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009942:Organometallic Compounds / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D012260:Ribonucleases / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods
D001578:Benzopyrans; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D011506:Proteins; C063917:SNARF dye; C521584:Zn(II)-DpaTyr; D012260:Ribonucleases