title: Characterization of cytosolic glutathione S-transferases in California Halibut (Paralichthys californicus).
abstract: Two cytosolic glutathione S-transferase (GST) classes were isolated and characterized from California halibut (Paralichthys californicus) liver. Nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to elucidate peptide sequences and the proteins were identified as theta and alpha by searching against the NCBI non-redundant database (nrDB). Catalytic activity of the cytosolic GSTs towards 1-chloro-2,4-dinitrobenzene (CDNB) was determined to be 0.23+/-0.003 U/mg cytosolic protein.
authors.affiliation: Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California, 1 Shields Avenue, Davis, CA 95616-8588, USA.
authors.forename: Rachel T
authors.initials: RT
authors.lastname: Donham
authors.forename: Andrea D
authors.initials: AD
authors.lastname: Luna
authors.forename: Sandra
authors.initials: S
authors.lastname: Chang
authors.forename: Dexter
authors.initials: D
authors.lastname: Morin
authors.forename: William T
authors.initials: WT
authors.lastname: Jewell
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Tjeerdema
mesh_terms: D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004137:Dinitrochlorobenzene; D005432:Flounder / Q000378:metabolism*; D005982:Glutathione Transferase / Q000302:isolation & purification / Q000378:metabolism*; D022781:Hepatocytes / Q000201:enzymology
chemical_list: D004137:Dinitrochlorobenzene; D005982:Glutathione Transferase