title: Occurrence of A-kinase anchor protein and associated cAMP-dependent protein kinase in the inner compartment of mammalian mitochondria.
abstract: Evidence showing the existence in the inner compartment of rat-heart mitochondria of AKAP121 and associated PKA is presented. Immunoblotting analysis and trypsin digestion pattern show that 90% or more of mitochondrial C-PKA, R-PKA and AKAP121 is localized in the inner mitochondrial compartment, when prepared both from isolated mitochondria or cardiomyocyte cultures. This localization is verified by measurement of the specific catalytic activity of PKA, radiolabelling of R-PKA by (32)P-phosphorylated C-PKA and of AKAP by (32)P-phosphorylated R-PKA and electron microscopy of mitochondria exposed to gold-conjugated AKAP121 antibody.
authors.affiliation: Department of Medical Biochemistry, Biology and Physics, University of Bari, Policlinico, Piazza Giulio Cesare 11, 70124 Bari, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Sardanelli
authors.forename: Anna
authors.initials: A
authors.lastname: Signorile
authors.forename: Rosanna
authors.initials: R
authors.lastname: Nuzzi
authors.forename: Domenico De
authors.initials: DD
authors.lastname: Rasmo
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Technikova-Dobrova
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Drahota
authors.forename: Antonella
authors.initials: A
authors.lastname: Occhiello
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pica
authors.forename: Sergio
authors.initials: S
authors.lastname: Papa
mesh_terms: D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D008929:Mitochondria, Heart / Q000378:metabolism* / Q000648:ultrastructure; D051336:Mitochondrial Membranes / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding; D051381:Rats
chemical_list: D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing; C486332:Akap1 protein, rat; D017868:Cyclic AMP-Dependent Protein Kinases