title: Distinct gene expression profiles in gastric epithelial cells induced by different clinical isolates of Helicobacter pylori--implication of bacteria and host interaction in gastric carcinogenesis.
abstract: OBJECTIVE
  Helicobacter pylori (H. pylori) infection is a major risk factor of peptic ulcer, gastric cancer, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma. The interplay between H. pylori and host is an important issue for elucidation of pathogenesis of H. pylori-related diseases. We aimed to examine simultaneously dynamic changes of multiple molecular pathways of infection affected by different H. pylori strains by cDNA microarrays.
  
  
  METHODS
  To elucidate the cross-talk between H. pylori and gastric epithelial cells, we isolated three different H. pylori strains from patients with gastric cancer (GC), duodenal ulcer (DU), and gastric MALT lymphoma (MA). The bacteria were co-cultured with gastric epithelial cells (AGS) and total RNAs were extracted from AGS cells and used for detection of genes represented in the microarray.
  
  
  RESULTS
  Of the 12,814 clones on the microarray, there were 522 genes expressed differently in the three groups. Of the 522 genes, there were 4 genes, 4 genes and 13 genes, either up- or down-regulated more than twofold change, in AGS cells induced specifically by GC, MA, and DU strain, respectively. The GC and DU strains induced more genes involving in carcinogenesis, such as pim-1, jun B, and VEGF.
  
  
  CONCLUSIONS
  Our data by cDNA microarray suggest bacterial factors may determine the outcomes of H. pylori infection. The expression profiles of cDNA microarray provide clues for diagnosis, treatment, and prevention of H. pylori-related gastroduodenal diseases.
authors.affiliation: Department of Internal Medicine, National Taiwan University Hospital College of Medicine, National Taiwan University, Taipei.
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Chang
authors.forename: Ming-Shiang
authors.initials: MS
authors.lastname: Wu
authors.forename: Ya-Jen
authors.initials: YJ
authors.lastname: Chang
authors.forename: Ching-Chow
authors.initials: CC
authors.lastname: Chen
authors.forename: Yi-Shing
authors.initials: YS
authors.lastname: Lin
authors.forename: Tracy
authors.initials: T
authors.lastname: Hsieh
authors.forename: Pan-Chyr
authors.initials: PC
authors.lastname: Yang
authors.forename: Jaw-Town
authors.initials: JT
authors.lastname: Lin
mesh_terms: D045744:Cell Line, Tumor; D004381:Duodenal Ulcer / Q000382:microbiology; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology; D005753:Gastric Mucosa / Q000166:cytology* / Q000382:microbiology; D015870:Gene Expression; D020869:Gene Expression Profiling; D016481:Helicobacter Infections / Q000235:genetics / Q000378:metabolism; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D018442:Lymphoma, B-Cell, Marginal Zone / Q000382:microbiology; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000382:microbiology
chemical_list: D011506:Proteins