[Evaluation of sperm mitochondrial function using Rh123/PI dual fluorescent staining].
OBJECTIVE
To investigate the feasibility and clinical significance of detecting sperm mitochondrial function by using Rh123/PI dual fluorescent staining and flow cytometry analysis, and to explore the relationship between the results of Rh123/PI dual fluorescent staining and seminal parameters.


METHODS
Sixty-three semen samples were classified as normal (n=31) and abnormal (n=32) according to the World Health Organization guidelines. Rh123/PI dual fluorescent staining was then carried out to evaluate sperm mitochondrial function by flow cytometry analysis.


RESULTS
Significant differences in Rh123+ PI-, Rh123- /PI+ and Rh123- /PI- were detected between the normal and abnormal semen samples (P < 0.05). There was a significant positive correlation between the Rh123+ PI- sperm and sperm motility and a significant inverse correlation between Rh123+ PI- and immotile sperm. But the Rh123- PI+ sperm showed a contrary relationship with Rh123+ PI-. A significant inverse correlation was also observed between the Rhl23- /PI- sperm and sperm concentration in the abnormal group.


CONCLUSIONS
Rh123/PI dual fluorescent staining and flow cytometry analysis can readily and quickly detect sperm mitochondrial function and be used to evaluate semen quality.
Wu
Yong-ming
YM
Laboratory of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, Nanjing, Jiangsu 210002, China. wuyongming8088@163.com
Xia
Xin-yi
XY
Pan
Lian-jun
LJ
Shao
Yong
Y
Jin
Bao-fang
BF
Huang
Yu-feng
YF
Wang
Xiu-lai
XL
D000328:Adult; D016022:Case-Control Studies; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000502:physiology*; D020112:Rhodamine 123; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa; D013194:Staining and Labeling / Q000379:methods*
D020112:Rhodamine 123