Establishment of an HPA-1- to -16-typed platelet donor registry in China.
In order to determine gene frequencies of human platelet antigen (HPA) and establish a panel of accredited HPA-1a, -2a, -4a, -5a and -6a-negative donors as well as an HPA-typed platelet donor registry, a total of 1000 Chinese donors of Han nationality (500 from north China and 500 from south China) were typed for HPA-1 through -16 using a DNA-based polymerase chain reaction with sequence-specific primers genotyping method. The gene frequencies of HPA-1b, -2b, -3b, -4b, -5b, -6bw, -10bw and -15b were 0.0060, 0.0485, 0.4055, 0.0045, 0.0140, 0.0135, 0.0005 and 0.4680, respectively. The HPA-7bw, -8bw, -9bw, -11bw, -12bw, -13bw, -14bw and -16bw alleles were not found. The HPA-2b and -5b homozygous donors were detected at low frequencies. The HPA mismatch probabilities potentially leading to alloimmunization in random platelet transfusion vary with a region from 0.1% to 37% depending on the distribution patterns of common and less common alleles in each system. This study provides a useful HPA-typed plateletpheresis donor registry in China and could improve platelet antibody detection and HPA-matched platelet transfusion in alloimmune thrombocytopenic patients.
Feng
M L
ML
Shanghai Blood Center, Shanghai, China. fengml@sh163.net
Liu
D Z
DZ
Shen
W
W
Wang
J L
JL
Guo
Z H
ZH
Zhang
X
X
Du
K M
KM
Qian
K C
KC
Zhao
T M
TM
D016824:Antigens, Human Platelet / Q000145:classification / Q000235:genetics*; D001782:Blood Donors / Q000600:supply & distribution; D002681:China / Q000208:ethnology; D005787:Gene Frequency / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D017713:Platelet Transfusion; D010983:Plateletpheresis; D012042:Registries
D016824:Antigens, Human Platelet