title: The crystal structure of L-lactate oxidase from Aerococcus viridans at 2.1A resolution reveals the mechanism of strict substrate recognition.
abstract: L-Lactate oxidase (LOX) from Aerococcus viridans is a member of the alpha-hydroxyacid-oxidase flavoenzyme family. We have determined the three-dimensional structure of LOX and revealed the mechanism of substrate recognition. The LOX monomer structure has a typical alpha(8)/beta(8) motif commonly found in other flavin family proteins. A related enzyme, glycolate oxidase, catalyzes the oxidation of glycolate rather than lactate. Comparison of the two enzyme structures highlights the importance of five residues around the FMN prosthetic group of LOX, which act synergistically to discriminate between the l/d configurations of lactate. X-ray crystallography of LOX gave a space group I422 of unit-cell parameters a=b=191.096A, c=194.497A and alpha=beta=gamma=90 degrees with four monomers per asymmetric unit. The four independent monomers display slight structural differences around the active site. Diffraction data were collected, under cryogenic conditions to 2.1A resolution at the synchrotron facilities in Japan.
authors.affiliation: Research Reactor Institute, Kyoto University, Kumatori, Osaka 590-0494, Japan.
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Umena
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yorita
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsuoka
authors.forename: Akiko
authors.initials: A
authors.lastname: Kita
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Fukui
authors.forename: Yukio
authors.initials: Y
authors.lastname: Morimoto
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D017385:Sequence Homology; D013289:Streptococcaceae / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D006899:Mixed Function Oxygenases; D000429:Alcohol Oxidoreductases; C020821:glycollate oxidase; C021497:lactate 2-monooxygenase