title: Protective immunity induced by phage displayed mitochondrial related peptides of Schistosoma japonicum.
abstract: New antigens and strategies are necessary for vaccine development against schistosomiasis japonica. Using a pool of 43 high titred anti-SWA sera from individuals residing in an Schistosoma japonicum endemic area of China, we have cloned a S. japonicum gene by cDNA library screening. The recombinant Sj338 protein has 44-46% identity to a mitochondrial precursor receptor protein of humans and rats. Immunization of mice with the recombinant Sj338 conferred 27-32% (p<0.01) reduction in worm burdens following cercarial challenge. In an effort to identify protective epitopes in Sj338 and increase the level of protection, we screened a random 12-mer peptide library constructed in M13 using a polyspecific anti-Sj338 rabbit serum. After five rounds of biopanning, we identified 30 reactive clones consisting of 11 distinct peptide sequences. These clones shared limited primary sequence homology with the recombinant Sj338 protein. Anti-sera raised against these phage clones recognized recombinant Sj338 and SWAP by Western blot. In murine vaccination experiments using whole recombinant phage without adjuvant, four of these clones demonstrated worm reductions of 11.6-25.1% (p=ns - 0.05) compared to M13 vaccinated animals. Animals vaccinated with all four of these phage demonstrated 34.2% (p<0.01) worm reduction compared to controls vaccinated with M13 clone. These data suggest that mimotope peptides are potential vaccine candidates for S. japonicum.
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Rd, Nanjing, Jiangsu 210029, PR China. haiwei@njmu.edu.cn
authors.forename: Hai-Wei
authors.initials: HW
authors.lastname: Wu
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Hu
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: J D
authors.initials: JD
authors.lastname: Kurtis
authors.forename: Fan-Jie
authors.initials: FJ
authors.lastname: Zeng
authors.forename: S T
authors.initials: ST
authors.lastname: McGarvey
authors.forename: Guan-Ling
authors.initials: GL
authors.lastname: Wu
authors.forename: Zhao-Song
authors.initials: ZS
authors.lastname: Zhang
authors.forename: Zi-Chun
authors.initials: ZC
authors.lastname: Hua
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005260:Female; D007114:Immunization / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D024101:Mitochondrial Proteins / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D019151:Peptide Library; D018510:RNA, Helminth; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000517:prevention & control*; D017422:Sequence Analysis, DNA
chemical_list: D000947:Antigens, Helminth; D000939:Epitopes; D024101:Mitochondrial Proteins; D019151:Peptide Library; D018510:RNA, Helminth