[Protective effect of reduced glutathione on cytotoxicity induced by hexavalent chromium [Cr(VI)] in L-02 hepatocyte].
OBJECTIVE
To explore the effect of reduced glutathione (GSH) on cytotoxicity induced by hexavalent chromium (Cr(VI)) in L-02 hepatocyte.


METHODS
The test has three groups:the groups of (Cr(VI), the groups of GSH, the groups of Cr (VI) and GSH. The survival rate of L-02 hepatocyte is assessed on the reductions of tetrazolium dye (MTT).


RESULTS
Significant cytotoxicities of L-02 hepatocyte were observed at the concenations of 2,4,8,16,32 and 64 micromol/L Cr (VI). Concentration-dependent decrease in cell survival rate of Cr (VI)-treated L-02 hepatocytes were observed (r = -0.910) Protective effect on all concentrations of Cr(VI) (2 - 64 micromol/L) at the dose of 20 micromol/LGSH were found.


CONCLUSIONS
The results demonstrated that proper concentrations of GSH could have protective effect on cytoxicity induced by Cr(VI) in L-02 hepatocyte. GSH of too low or too high concentrations don't has this effect.
Wang
Hai-hua
HH
Institute for Occupational Health and Poison Control, China CDC, Beijing 100050, China.
Xiao
Jing-wei
JW
Li
Bin
B
Zhong
Cai-gao
CG
D002460:Cell Line; D002857:Chromium / Q000633:toxicity*; D005978:Glutathione / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D020011:Protective Agents / Q000494:pharmacology*
D020011:Protective Agents; D002857:Chromium; C074702:chromium hexavalent ion; D005978:Glutathione