title: Improvement strategies in ovine artificial insemination.
abstract: Artificial insemination in ram is scarcely widespread comparing with other domestic species. This has been due not only to fertility results being irregular and low but also because of the difficulty in the application of enhancements such as the use of frozen-thawed sperm. Although there is a lot of information on the use of different options to improve these AI results (such as transcervical application, the use of thawed sperm, etc.) commercial programmes can be classified on two general categories: those using refrigerated semen (15 degrees C) by superficial intracervical deposition (vaginal), and, more restricted, those using thawed sperm by intrauterine deposition (laparoscopy). In the present work, we have summarized our viewpoint on three general research lines for the improvement of AI results in sheep: semen preservation, AI procedures and semen assessment. Briefly, in ram it is necessary to develop a medium term methodology of sperm refrigeration (3-5 days), which would allow the distribution of sperm doses to a widespread area. Nevertheless, it is also necessary to develop an intrauterine transcervical AI technique, which allows thawed semen to be applied by vaginal insemination. Besides, the low predictive value of classic assessment techniques limits the ability to adjust the number of spermatozoa per dose according to its actual fertility.
authors.affiliation: Animal Reproduction and Obstetrics, University of León, Leon, Spain. dsalar@unileon.es
authors.forename: L
authors.initials: L
authors.lastname: Anel
authors.forename: M
authors.initials: M
authors.lastname: Alvarez
authors.forename: F
authors.initials: F
authors.lastname: Martinez-Pastor
authors.forename: V
authors.initials: V
authors.lastname: Garcia-Macias
authors.forename: E
authors.initials: E
authors.lastname: Anel
authors.forename: P
authors.initials: P
authors.lastname: de Paz
mesh_terms: D000818:Animals; D002470:Cell Survival; D005260:Female; D005298:Fertility / Q000502:physiology*; D007315:Insemination, Artificial / Q000662:veterinary*; D008297:Male; D012034:Refrigeration; D012662:Semen Preservation / Q000009:adverse effects / Q000379:methods / Q000662:veterinary*; D012756:Sheep / Q000502:physiology*; D013076:Sperm Count / Q000662:veterinary; D013094:Spermatozoa / Q000502:physiology*