AtGRP7 is involved in the regulation of abscisic acid and stress responses in Arabidopsis.
The Arabidopsis AtGRP7 gene, encoding a glycine-rich RNA-binding protein, has been shown to be involved in the regulation of a circadian-regulated negative feedback loop. However, little is known about the role of AtGRP7 in mediating abscisic acid (ABA) and stress responses. Here, we show that AtGRP7 plays a role in both. AtGRP7 was repressed by ABA, high salt and mannitol. Disruption of AtGRP7 by T-DNA insertion led to hypersensitive responses to ABA in both seed germination and root growth assays. The atgrp7-1 mutant was also hypersensitive to osmotic stress conditions, such as high salt and high concentrations of mannitol. In addition, the atgrp7-1 mutant plants accumulated significantly higher transcript levels of two ABA-and stress-inducible genes, RD29A and RAB18, compared with the wild-type plants. Taken together, these results suggest that AtGRP7 is involved in the regulation of ABA and stress responses.
Cao
Shuqing
S
School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui, China. shuqing.cao@163.com
Jiang
Li
L
Song
Shiyong
S
Jing
Ran
R
Xu
Guosheng
G
D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D008353:Mannitol / Q000378:metabolism; D009997:Osmotic Pressure; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D012965:Sodium Chloride / Q000378:metabolism
D000040:Abscisic Acid; D029681:Arabidopsis Proteins; D008353:Mannitol; D016601:RNA-Binding Proteins; D012965:Sodium Chloride; C118496:GRP7 protein, Arabidopsis