Determination of the neuropeptides arginine vasotocin and isotocin in brains of three-spined sticklebacks (Gasterosteus aculeatus) by off-line solid phase extraction-liquid chromatography-electrospray tandem mass spectrometry.
A method based on solid phase extraction (SPE) followed by liquid chromatography-electrospray ionisation tandem mass spectrometry for the determination of the nonapeptides arginine vasotocin (AVT) and isotocin (IT) in brains of three-spined sticklebacks (Gasterosteus aculeatus) is described. Separation and detection were optimized using synthetic standards. Limits of detection (LOD) for standard solutions were 160 pg mL(-1) for AVT and 250 pg mL(-1) for IT. The SPE procedure hardly affected the LODs for standard solutions. Mainly because of ion suppression, LODs for AVT and IT in brains were approximately 5 and 25 pg mg(-1), respectively. The concentrations determined in the brain of several fishes ranged from 10 to 500 pg mg(-1) for AVT and from 400 to 4000 pg mg(-1) for IT.
Kleszczyńska
Agnieszka
A
Department of Genetics and Marine Biotechnology, Institute of Oceanology of Polish Academy of Sciences, Powstańców Warszawy 55 Str, 81-712 Sopot, Poland.
Dumont
Emmie
E
Cornelis
Rita
R
Gozdowska
Magdalena
M
Kulczykowska
Ewa
E
Sandra
Pat
P
D000818:Animals; D001923:Brain Chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D023701:Smegmamorpha / Q000378:metabolism*; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014668:Vasotocin / Q000032:analysis* / Q000737:chemistry
D010121:Oxytocin; C008215:isotocin; D014668:Vasotocin