[Production and characterization of gold labeled monoclonal antibody against MC-LR].
OBJECTIVE
Production of the gold labeled monoclonal antibody against MC-LR. Study the simple mechanism of the conjugation by the spectral analysis.


METHODS
Production and purification monoclonal antibodies against MC-LR by hybridoma technology. Production the 20nm colloidal gold and label the monoclonal antibody. Study the gold--McAb conjugate by the spectroanalysis.


RESULTS
Monoclonal antibody produced by the hybridoma cells were tested for subtypes and designated as IgG2a for 8C11. The titers of antibody in ascites was 108.The IC50 for MC-LR was around 3ng/ml. The IgG molecule weight was 1.5 x 10(5). The structure of the antibody was altered to more tighter and stabler.


CONCLUSIONS
Product the gold labeled monoclonalantibody against MC-LR conjugate successfully and keep the antige-binding determinant.
Zhao
Xiao-lian
XL
Southern Yangtz University, Wuxi 214036, China.
Sun
Wei-rong
WR
Sun
Xiu-lan
XL
Jiang
Tao
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D017979:Gold Colloid; D006825:Hybridomas; D007118:Immunoassay / Q000379:methods; D008387:Marine Toxins; D051379:Mice; D008807:Mice, Inbred BALB C; D052998:Microcystins / Q000032:analysis* / Q000276:immunology*; D011934:Reagent Strips
D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D008387:Marine Toxins; D052998:Microcystins; D011934:Reagent Strips; C057862:cyanoginosin LR