Application of Evidence Investigator for the simultaneous measurement of soluble adhesion molecules: L-, P-, E- selectins, VCAM-1, ICAM-1 in a biochip platform.
The semiautomated Evidence Investigator has been applied to the simultaneous specific measurement of soluble adhesion molecules: L-, P-, E- selectins, VCAM-1, ICAM-1 using a reduced volume of sample. The biochip is the solid support and vessel where the sandwich immunoassay takes place. Signal detection, imaging, data processing, and storage are fully automated. Calibration curves are generated simultaneously for each analyte, with automatic validation against supplied calibration data. These curves are used for the calculation of the concentrations in multi-analyte controls and human serum samples. Data from the evaluation parameters assessed indicate suitability of the Evidence Investigator system for the application.
McAleer
Damien
D
Randox Laboratories, 55 Diamond Rd., Crumlin, County Antrim BT29 4QY, United Kingdom.
McPhillips
Frances M
FM
FitzGerald
Stephen P
SP
McConnell
Robert I
RI
Rodriguez
Maria-Luz
ML
D015415:Biomarkers / Q000097:blood / Q000737:chemistry; D002138:Calibration; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood* / Q000737:chemistry; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D019087:Selectins / Q000097:blood* / Q000737:chemistry; D012680:Sensitivity and Specificity; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood* / Q000737:chemistry
D015415:Biomarkers; D019087:Selectins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1