title: Pyrazolidine-3,5-diones and 5-hydroxy-1H-pyrazol-3(2H)-ones, inhibitors of UDP-N-acetylenolpyruvyl glucosamine reductase.
abstract: A series of pyrazolidine-3,5-dione and 5-hydroxy-1H-pyrazol-3(2H)-one inhibitors of Escherichia coli UDP-N-acetylenolpyruvyl glucosamine reductase (MurB) has been prepared. The 5-hydroxy-1H-pyrazol-3(2H)-ones show low micromolar IC(50) values versus E. coli MurB and submicromolar minimal inhibitory concentrations (MIC) against Staphylococcus aureus GC 1131, Enterococcus faecalis GC 2242, Streptococcus pneumoniae GC 1894, and E. coli GC 4560 imp, a strain with increased outer membrane permeability. None of these compounds show antimicrobial activity against Candida albicans, a marker of eukaryotic toxicity. Moreover, these compounds inhibit peptidoglycan biosynthesis, as assessed by measuring the amount of soluble peptidoglycan produced by Streptococcus epidermidis upon incubation with compounds. A partial least squares projection to latent structures analysis shows that improving MurB potency and MIC values correlate with increasing lipophilicity of the C-4 substituent of the 5-hydroxy-1H-pyrazol-3(2H)-one core. Docking studies using FLO and PharmDock produced several binding orientations for these molecules in the MurB active site.
authors.affiliation: Chemical and Screening Sciences and Infectious Diseases Research, Wyeth, 401 North Middletown Road, Pearl River, New York 10965, USA. gilbera@wyeth.com
authors.forename: Adam M
authors.initials: AM
authors.lastname: Gilbert
authors.forename: Amedeo
authors.initials: A
authors.lastname: Failli
authors.forename: Jay
authors.initials: J
authors.lastname: Shumsky
authors.forename: Youjun
authors.initials: Y
authors.lastname: Yang
authors.forename: Anatoly
authors.initials: A
authors.lastname: Severin
authors.forename: Guy
authors.initials: G
authors.lastname: Singh
authors.forename: William
authors.initials: W
authors.lastname: Hu
authors.forename: David
authors.initials: D
authors.lastname: Keeney
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Petersen
authors.forename: Alan H
authors.initials: AH
authors.lastname: Katz
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D002237:Carbohydrate Dehydrogenases / Q000037:antagonists & inhibitors*; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D010457:Peptidoglycan / Q000096:biosynthesis; D011720:Pyrazoles / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D010457:Peptidoglycan; D011720:Pyrazoles; D002237:Carbohydrate Dehydrogenases; C023215:UDP-N-acetylmuramate dehydrogenase