title: Effect of gypsum on proliferation and differentiation of MC3T3-E1 mouse osteoblastic cells.
abstract: Recently, calcium sulfate dihydrate has been demonstrated as safe biodegradable osteoconductive bone void filler. However, its exact mechanism of action on bone cells is yet unknown. In this study, the influence of gypsum on gene expression and proliferation of MC3T3-E1 mouse pre-osteoblastic cells was investigated. Cells were cultured on gypsum disc, slice, polymethylmethacrylate (PMMA), or plastic culture plate for 15 days. Cell viability, alkaline phosphatase (ALP) activity and expression profile of 15 genes involved in bone metabolism were measured in cultures. Cell proliferation on gypsum was increased by almost 2-fold, while an inhibitory effect of PMMA on proliferation rate of osteoblasts was noted. Cells cultured on gypsum disc surface exhibited an increased ALP activity and markedly different gene expression profile. Quantitative real-time PCR data indicated the expression of genes that might provide a basis for an osteoinductive potential. MC3T3-E1 cells expressed genes typical of bone fracture healing like type II collagen and fibronectin 1. These effects might be related to the calcium content of gypsum and mediated likely via SMAD3. Our results suggest that gypsum can support new bone formation by its calcium content and modulatory effect on gene expression profile of bone cells.
authors.affiliation: 1st Department of Medicine, Semmelweis University, Korányi S. u. 2/a, Budapest H-1083, Hungary. lazaron@bell.sote.hu
authors.forename: Aron
authors.initials: A
authors.lastname: Lazáry
authors.forename: Bernadett
authors.initials: B
authors.lastname: Balla
authors.forename: János P
authors.initials: JP
authors.lastname: Kósa
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bácsi
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Nagy
authors.forename: István
authors.initials: I
authors.lastname: Takács
authors.forename: Péter P
authors.initials: PP
authors.lastname: Varga
authors.forename: Gábor
authors.initials: G
authors.lastname: Speer
authors.forename: Péter
authors.initials: P
authors.lastname: Lakatos
mesh_terms: D016475:3T3 Cells; D000818:Animals; D016025:Bone Transplantation; D001842:Bone and Bones / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002133:Calcium Sulfate / Q000494:pharmacology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D003764:Dental Materials / Q000494:pharmacology; D005786:Gene Expression Regulation; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D051900:Smad3 Protein / Q000378:metabolism
chemical_list: D003764:Dental Materials; D051900:Smad3 Protein; D002118:Calcium; D002133:Calcium Sulfate