title: Canola (Brassica napus L.).
abstract: High-frequency Agrobacterium tumefaciens-mediated transformation can be obtained in canola by optimizing the preconditioning time of the explant and cocultivation time with A. tumefaciens. A preconditioning time of 72 h and cocultivation of 48 h synergistically increase the transformation efficiency to 25%. In addition, the recovery of transgenic plants can be facilitated by overcoming hyperhydration, which increases the rooting frequency to 100%.
authors.affiliation: Department of Botany, University of Vermont, Burlington, VT, USA.
authors.forename: Vinitha
authors.initials: V
authors.lastname: Cardoza
authors.forename: C Neal
authors.initials: CN
authors.lastname: Stewart
mesh_terms: D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D029688:Brassica napus / Q000166:cytology / Q000235:genetics* / Q000382:microbiology; D018920:Coculture Techniques; D018014:Gene Transfer Techniques; D030821:Plants, Genetically Modified / Q000196:embryology / Q000235:genetics* / Q000382:microbiology; D013997:Time Factors; D014170:Transformation, Genetic