title: Structure of the O-polysaccharide of Escherichia coli O150 containing 2-acetamido-4-O-[(S)-1-carboxyethyl]-2-deoxy-d-glucose.
abstract: An acidic O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Escherichia coli O150 and studied by sugar and methylation analyses, triflic acid solvolysis, Smith degradation, (1)H and (13)C NMR spectroscopy, including 2D ROESY, (1)H,(13)C HSQC, HMQC-TOCSY, and HMBC experiments. The polysaccharide was found to contain a regioisomer of N-acetylisomuramic acid, 2-acetamido-4-O-[(S)-1-carboxyethyl]-2-deoxy-d-glucose [d-GlcNAc4(Slac)]. The structure of its hexasaccharide repeating unit was established.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.forename: Weiqing
authors.initials: W
authors.lastname: Han
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: Sergei D
authors.initials: SD
authors.lastname: Shevelev
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Lu
authors.initials: L
authors.lastname: Feng
authors.forename: Yanqun
authors.initials: Y
authors.lastname: Liu
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry*; D008969:Molecular Sequence Data; D009112:Muramic Acids / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D009112:Muramic Acids; D019081:O Antigens; C031651:N-acetylmuramic acid