Expressions of polypeptide: N-acetylgalactosaminyltransferase in leukemia cell lines during 1,25-dihydroxyvitamin D3 induced differentiation.
The effect of 1,25-dihydroxyvitamin D3 [1,25(OH)(2)D3] on two leukemia cell lines, K562 and SHI-1, and its relation to the expression of different subtypes of polypeptide: N-acetylgalactosaminyltransferase (pp-GalNAc-T) was studied. With morphological and cell flow-cytometric method, it was found that 1,25(OH)(2)D3 induced the differentiation of both leukemia cell lines toward monocytic lineage, but not affected the cell growth and apoptosis. The expressions of different subtypes of pp-GalNAc-T, the initial glycosyltransferase in O-glycan synthesis, were studied with RT-PCR before and after the treatment of different concentrations of 1,25(OH)(2)D3. Among fourteen subtypes of pp-GalNAc-T (T1 approximately T14), K562 cells obviously expressed pp-GalNAc-T2, T4, T5, T7 (T2 was the highest) and SHI-1 cells apparently expressed pp-GalNAcT1, T2, T3 and T4 (T4 was the highest) only. After K562 cells were treated 1, 25(OH)(2)D3 for 72 h, pp-GalNAc-T2, T4, T5, T7 were increased in a dose dependent manner. In contrast, pp-GalNAc-T1 and T2, especially T1, were up-regulated in SHI-1 cells by 1,25(OH)(2)D3, but T3 was unchanged and T4 was down-regulated. The different alterations of pp-GalNAc-Ts in these two cell lines were probably related to the different structural changes of O-glycans during 1,25(OH)(2)D3 induced differentiation.
Qiu
Hao
H
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Institute of Bioengineering, Suzhou University, Suzhou, China.
Guo
Xiang-Hong
XH
Mo
Jian-Hua
JH
Jin
Mei-Fang
MF
Wu
Shi-Liang
SL
Chen
Hui-Li
HL
D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D002117:Calcitriol / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D020014:K562 Cells; D007938:Leukemia / Q000188:drug therapy* / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics* / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097763:Polypeptide N-acetylgalactosaminyltransferase
D002117:Calcitriol; D017931:DNA Primers; D007527:Isoenzymes; D017350:N-Acetylgalactosaminyltransferases; D011134:Polysaccharides; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D018167:Receptors, Calcitriol; D000097763:Polypeptide N-acetylgalactosaminyltransferase