Expression profiling characterization of laminin alpha-2 positive MDC.
In the Caucasian population, patients affected by the most frequent forms of congenital muscular dystrophies (MDC) are commonly divided into two groups. The first is characterized by mutations of the gene for the laminin alpha-2 (LAMA2). The second is positive for this protein, highly heterogeneous, and has no specific genetic defect associated yet. We studied the skeletal muscle transcriptome of four LAMA2 deficient and six LAMA2 positive MDC patients by cDNA microarrays. The expression profiling defined two patients groups: one mild and one severe phenotype. This result was in agreement with histopathological features but only partially with the clinical classification. The mild phenotype is characterized by a delayed maturation from slow to fast muscle fibers. Other muscle transcripts, such as telethonin, myosin light-chains 3 and 1V, are underexpressed in this group. We suggest that expression profiling will provide important information to improve our understanding of the molecular basis of laminin alpha-2 positive MDC.
Millino
Caterina
C
CRIBI Biotechnology Center and Dipartimento di Biologia, Università degli Studi di Padova, Padova, Italy.
Bellin
Milena
M
Fanin
Marina
M
Romualdi
Chiara
C
Pegoraro
Elena
E
Angelini
Corrado
C
Lanfranchi
Gerolamo
G
D003402:Creatine Kinase / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007797:Laminin / Q000032:analysis* / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D009136:Muscular Dystrophies / Q000151:congenital* / Q000235:genetics* / Q000378:metabolism
D007797:Laminin; C102645:laminin alpha 2; D003402:Creatine Kinase