title: MHC class II molecules control murine B cell responsiveness to lipopolysaccharide stimulation.
abstract: LPS is a strong stimulator of the innate immune system and inducer of B lymphocyte activation. Two TLRs, TLR4 and RP105 (CD180), have been identified as mediators of LPS signaling in murine B cells, but little is known about genetic factors that are able to control LPS-induced cell activation. We performed a mouse genome-wide screen that aside from identifying a controlling locus mapping in the TLR4 region (logarithm of odds score, 2.77), also revealed that a locus closely linked to the MHC region (logarithm of odds score, 3.4) governed B cell responsiveness to LPS stimulation. Using purified B cells obtained from MHC congenic strains, we demonstrated that the MHC(b) haplotype is accountable for higher cell activation, cell proliferation, and IgM secretion, after LPS stimulation, when compared with the MHC(d) haplotype. Furthermore, B cells from MHC class II(-/-) mice displayed enhanced activation and proliferation in response to LPS. In addition, we showed that the MHC haplotype partially controls expression of RP105 (a LPS receptor molecule), following a pattern that resembles the LPS responsiveness phenotype. Together, our results strongly suggest that murine MHC class II molecules play a role in constraining the B cell response to LPS and that genetic variation at the MHC locus is an important component in controlling B cell responsiveness to LPS stimulation. This work raises the possibility that constraining of B cell responsiveness by MHC class II molecules may represent a functional interaction between adaptive and innate immune systems.
authors.affiliation: Instituto Gulbenkian de Ciência, P-7281-901 Oeiras, Portugal.
authors.forename: Joana
authors.initials: J
authors.lastname: Rodo
authors.forename: Lígia A
authors.initials: LA
authors.lastname: Gonçalves
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Demengeot
authors.forename: António
authors.initials: A
authors.lastname: Coutinho
authors.forename: Carlos
authors.initials: C
authors.lastname: Penha-Gonçalves
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology*; D005802:Genes, MHC Class II; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008070:Lipopolysaccharides / Q000276:immunology*; D008126:Lod Score; D008213:Lymphocyte Activation / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci
chemical_list: D015703:Antigens, CD; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; C490500:Ly78 protein, mouse