Evaluation of IGL-1 preservation solution using an orthotopic liver transplantation model.
OBJECTIVE
To compare, in a pig liver transplantation model, the protective effect of UW with that of IGL-1, a high-sodium preservation solution containing polyethylene glycol (PEG) as an oncotic supply.


METHODS
All livers were harvested and grafted orthotopically according to standard techniques. The livers were washed out and preserved for 7 h in IGL-1 (n = 6) or in UW solution (n = 7) at 4 degree centigrade. In a sham group (n = 4), the livers underwent a 60-min warm ischemia at 37 degree centigrade. The hepatocellular injury was assessed in organ preservation solution washed out from the graft at the end of ischemic storage (before revascularization), and in serum 2 h after reperfusion and daily for up to 6 d.


RESULTS
Livers preserved in IGL-1 solution released markedly less AST than that preserved in the UW solution before and after revascularization (P < 0.05). Besides, the activity of creatine kinase-BB, a marker of sinusoidal lining cells injury, was higher in the UW group than in the IGL-1 group (P < 0.05). Histological results showed less necrotic regions in livers preserved in IGL-1 solution; however, no difference was observed for inflammation.


CONCLUSIONS
IGL-1 liquid effectively protects parenchymal and non-parenchymal cells against preservation-reperfusion injuries.
Ben Abdennebi
Hassen
H
Department of Humane Physiology, Faculty of Pharmacy, Monastir 5000, Tunisia. hbenabdennebi@yahoo.fr
Elrassi
Ziad
Z
Scoazec
Jean-Yves
JY
Steghens
Jean-Paul
JP
Ramella-Virieux
Silvina
S
Boillot
Olivier
O
D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005260:Female; D007511:Ischemia; D016031:Liver Transplantation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry; D015427:Reperfusion Injury / Q000517:prevention & control; D013552:Swine; D013997:Time Factors
C504757:IGL-1 solution; D019188:Organ Preservation Solutions; D011092:Polyethylene Glycols; D003402:Creatine Kinase