Optimized candidal biofilm microtiter assay.
Microtiter based candidal biofilm formation is commonly being used. Here we describe the analysis of factors influencing the development of candidal biofilms such as the coating with serum, growth medium and pH. The data reported here show that optimal candidal biofilm formation is obtained when grown in unbuffered YNB at pH 7, in wells that have been coated with Fetal Calf Serum or Fetal Bovine Serum.
Krom
Bastiaan P
BP
Department of BioMedical Engineering, University Medical Center Groningen and the University of Groningen, Groningen, The Netherlands. b.p.krom@med.umcg.nl
Cohen
Jesse B
JB
McElhaney Feser
Gail E
GE
Cihlar
Ronald L
RL
D001422:Bacterial Adhesion / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D002176:Candida albicans / Q000502:physiology*; D002177:Candidiasis / Q000382:microbiology*; D005562:Formazans / Q000737:chemistry; D006801:Humans; D044967:Serum / Q000382:microbiology / Q000502:physiology; D013778:Tetrazolium Salts / Q000737:chemistry
D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan