title: Identification of Tctex2beta, a novel dynein light chain family member that interacts with different transforming growth factor-beta receptors.
abstract: Endoglin is a membrane-inserted protein that is preferentially synthesized in angiogenic vascular endothelial and smooth muscle cells. Endoglin associates with members of the transforming growth factor-beta (TGF-beta) receptor family and has been identified as the gene involved in hereditary hemorrhagic telangiectasia. Although endoglin is known to affect cell responses to TGF-beta, its mode of action is largely unknown. We performed yeast two-hybrid screening of a human placental cDNA library and isolated a new endoglin-binding partner, a novel 221-amino acid member of the Tctex1/2 family of cytoplasmic dynein light chains named Tctex2beta, as the founder of a new Tctex1/2 subfamily. The interaction was localized exclusively to the cytoplasmic domain of endoglin. Reverse transcription-PCR showed expression of Tctex2beta in a wide range of tissues, including vascular endothelial and smooth muscle cells, placenta, and testis, as well as in several tumor cell lines. High expression levels were found in human umbilical vein endothelial cells and the large cell lung cancer cell line. Forced expression of Tctex2beta had a profound inhibitory effect on TGF-beta signaling. Additional Tctex2beta-interacting receptors were identified to be the TGF-beta type II receptor and most likely beta-glycan, but not ALK5, ALK1, or the bone morphogenetic protein type II receptor. Upon fluorescence tagging, co-localization of Tctex2beta and endoglin, as well as Tctex2beta, endoglin, and the TGF-beta type II receptor, was observed by different microscopy techniques. Our findings link endoglin for the first time to microtubule-based minus end-directed transport machinery, suggesting that some endoglin functions might be regulated and directed by its interaction with the cytoplasmic dynein light chain Tctex2beta.
authors.affiliation: Cardiovascular Clinical Academic Group, University Department of Medicine, Manchester Royal Infirmary, Oxford Road, Manchester M13 9WL, United Kingdom.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Meng
authors.forename: Andreas
authors.initials: A
authors.lastname: Lux
authors.forename: Andreas
authors.initials: A
authors.lastname: Holloschi
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: John M X
authors.initials: JM
authors.lastname: Hughes
authors.forename: Tassilo
authors.initials: T
authors.lastname: Foerg
authors.forename: John E G
authors.initials: JE
authors.lastname: McCarthy
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Heagerty
authors.forename: Petra
authors.initials: P
authors.lastname: Kioschis
authors.forename: Mathias
authors.initials: M
authors.lastname: Hafner
authors.forename: John M
authors.initials: JM
authors.lastname: Garland
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000378:metabolism; D019556:COS Cells; D002352:Carrier Proteins / Q000737:chemistry*; D002522:Chlorocebus aethiops; D029721:Drosophila Proteins / Q000737:chemistry*; D004398:Dyneins; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D008907:Mink; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins / Q000502:physiology*; D010802:Phylogeny; D011485:Protein Binding; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D056427:t-Complex Genome Region
chemical_list: D002352:Carrier Proteins; D029721:Drosophila Proteins; C533386:Dynlt1b protein, mouse; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D018125:Receptors, Transforming Growth Factor beta; D052006:Bone Morphogenetic Protein Receptors, Type II; D004398:Dyneins