Experimental Coxiella burnetii infection in pregnant goats: a histopathological and immunohistochemical study.
Pregnant goats were inoculated subcutaneosly with Coxiella burnetii and the course of infection was studied. Abortion in the last third of pregnancy occurred in all infected animals. Tissues from the placenta and other organs were studied before and after abortion by immunohistochemistry and PCR analysis. After infection, mild lesions were observed in several maternal organs, mainly the mammary gland but also the lung and the liver. The trophoblast cells of the choriallantoic membrane were the first target cells of the placenta; there was, however, a substantial delay between initial infection and placental colonization. In the last weeks of pregnancy, just before abortion, massive bacterial multiplication was detected in the placenta. In this stage of infection a necrotic and suppurative placentitis separated the fetal trophoblast cells from maternal syncytial epithelium. Vasculitis was observed in the fetal mesenchyme. A strong maternal T-cell response was detected in the inter-placentomal areas but not in the placentomes, where only neutrophils and smaller numbers of macrophages were associated with the lesions. Neither lesions nor C. burnetii DNA were found in maternal organs in animals maintained until day 120 post-abortion.
Sánchez
J
J
Departamento de Anatomía y Anatomía Patológica Comparadas. Electronic address: jsanchez@um.es.
Souriau
A
A
Pathologie Infectieuse et Immunologie, INRA, Tours-Nouzilly, 37380 Nouzilly, France.
Buendía
A J
AJ
Departamento de Anatomía y Anatomía Patológica Comparadas.
Arricau-Bouvery
N
N
Pathologie Infectieuse et Immunologie, INRA, Tours-Nouzilly, 37380 Nouzilly, France.
Martínez
C M
CM
Departamento de Anatomía y Anatomía Patológica Comparadas.
Salinas
J
J
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Rodolakis
A
A
Pathologie Infectieuse et Immunologie, INRA, Tours-Nouzilly, 37380 Nouzilly, France.
Navarro
J A
JA
Departamento de Anatomía y Anatomía Patológica Comparadas.
D037881:Aborted Fetus / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D000028:Abortion, Induced / Q000662:veterinary; D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism; D049033:Chorioallantoic Membrane / Q000473:pathology; D016997:Coxiella burnetii / Q000472:pathogenicity*; D005260:Female; D015511:Goat Diseases / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D006041:Goats / Q000382:microbiology*; D018445:Infectious Disease Transmission, Vertical; D008099:Liver / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008321:Mammary Glands, Animal / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D010920:Placenta / Q000382:microbiology / Q000473:pathology; D011247:Pregnancy; D011778:Q Fever / Q000473:pathology / Q000662:veterinary*; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D014657:Vasculitis / Q000382:microbiology / Q000662:veterinary
D000942:Antigens, Bacterial