title: Supramolecular assembly of cyclodextrin-based nanoparticles on solid surfaces for gene delivery.
abstract: In this work, a new approach for surface-mediated gene delivery based on inclusion complex formation between the solid surface and delivery vehicles is presented. beta-Cyclodextrin (CD) molecules form high-affinity inclusion complexes with adamantane. This complexation ability was used to specifically immobilize beta-CD-modified poly(ethylenimine) (CD-PEI) nanoparticles on adamantane- (AD-) modified self-assembled monolayers. To investigate the nanoparticle/surface interaction, CD-PEI-based and PEI-based nanoparticles were passed through a surface plasmon resonance flow cell containing the monolayers. CD-PEI nanoparticles are specifically immobilized on the chip surface by cyclodextrin-adamantane inclusion complex formation. Minimal nanoparticle adsorption was detected with PEI-based nanoparticles or on control surfaces. Competition studies with free cyclodextrins reveal that the multivalent interactions between CD-PEI nanoparticles and the adamantane-modified surface results in significantly higher binding affinity than single cyclodextrin-adamantane complexes. Immobilized nanoparticles were characterized by atomic force microscopy and quantified by fluorescence assay. Thus, the ability of CD-PEI nanoparticles to form inclusion complexes can be exploited to attain specific, high-affinity loading of delivery vehicles onto solid surfaces.
authors.affiliation: Departments of Bioengineering and Chemical Engineering, University of Washington, Seattle, Washington 98195, USA.
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Park
authors.forename: Horst A
authors.initials: HA
authors.lastname: von Recum
authors.forename: Shaoyi
authors.initials: S
authors.lastname: Jiang
authors.forename: Suzie H
authors.initials: SH
authors.lastname: Pun
mesh_terms: D000218:Adamantane / Q000737:chemistry*; D018014:Gene Transfer Techniques; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D013499:Surface Properties; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; D000218:Adamantane