title: Kv4 channels sensitive to BmTX3 in rat nervous system: autoradiographic analysis of their distribution during brain ontogenesis.
abstract: The binding site distribution of sBmTX3, a chemically synthesized toxin originally purified from the venom of the scorpion Buthus martensi, was investigated in adult and developing rat brain, using patch-clamp experiments and quantitative autoradiography. The molecular basis of these sBmTX3 sites was analysed by electrophysiology on transient Kv currents recorded in mammalian transfected cells. The rapidly activating and inactivating Kv4.1 current was inhibited by sBmTX3 (IC50, 105 nM). The inhibition was less effective on Kv4.2 and Kv4.3 channels and the toxin did not affect other transient currents such as Kv1.4 and Kv3.4. The distribution of the 125I-sBmTX3 binding sites was heterogeneous, with a 113-fold difference between the highest and the lowest densities in adult rat brain. The site density was particularly important in the caudate-putamen and accumbens nucleus, thalamus, hippocampal formation and cerebellum. The affinity of sBmTX3 remained constant during brain ontogenesis. The level of sBmTX3 binding sites was very low in prenatal and postnatal stages to postnatal day (P)12 but drastically increased from P15 in the major part of the studied structures except in the CA3 hippocampal field where the density was very high from P6. Thus, the distribution of sBmTX3 binding sites in rat brain and its electrophysiological characteristics suggest that sBmTX3 specifically binds to the Kv4 subfamily of K channels.
authors.affiliation: CNRS, UMR 6149, Neurobiologie des Processus Mnésiques, Université de Provence, Pôle 3C, Centre St Charles, 3 Place Victor Hugo, 13331 Marseille cedex 03, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Vacher
authors.forename: Sylvie
authors.initials: S
authors.lastname: Diochot
authors.forename: Pierre E
authors.initials: PE
authors.lastname: Bougis
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Martin-Eauclaire
authors.forename: Christiane
authors.initials: C
authors.lastname: Mourre
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D019556:COS Cells; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation / Q000379:methods; D004622:Embryo, Mammalian; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D018408:Patch-Clamp Techniques / Q000379:methods; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012604:Scorpion Venoms / Q000494:pharmacology*; D051672:Shal Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D014018:Tissue Distribution / Q000187:drug effects / Q000502:physiology; D014162:Transfection / Q000379:methods
chemical_list: D012604:Scorpion Venoms; D051672:Shal Potassium Channels; C418406:TX3 toxin, Buthus martensii