title: Repeat motions and backbone flexibility in designed proteins with different numbers of identical consensus tetratricopeptide repeats.
abstract: The tetratricopeptide repeat (TPR) is a 34-residue helix-turn-helix motif that occurs as three or more tandem repeats in a wide variety of proteins. We have determined the repeat motions and backbone fluctuations of proteins containing two or three consensus TPR repeats (CTPR2 and CPTR3, respectively) using 15N NMR relaxation measurements. Rotational diffusion tensors calculated from these data for each repeat within each TPR protein indicate that there is a high degree of motional correlation between different repeats in the same protein. This is consistent with the prevailing view that repeat proteins, such as CTPR2 and CTPR3, behave as single cooperatively folded domains. The internal motions of backbone NH groups were determined using the Lipari-Szabo model-free formalism. For most residues, there was a clear separation between the influence of internal motion and the influence of global rotational tumbling on the observed magnetic relaxation. The local internal motions are highly restricted in most of the helical elements, with slightly greater flexibility in the linker elements. Comparisons between CTPR2 and CTPR3 indicate that an addition of a TPR repeat to the C-terminus (before the solvation helix) of CTPR2 slightly reduces the flexibility of the preceding helix.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, Indiana 47405-0001, USA.
authors.forename: Cecilia Y
authors.initials: CY
authors.lastname: Cheng
authors.forename: Virginia A
authors.initials: VA
authors.lastname: Jarymowycz
authors.forename: Aitziber L
authors.initials: AL
authors.lastname: Cortajarena
authors.forename: Lynne
authors.initials: L
authors.lastname: Regan
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Stone
mesh_terms: D018257:Helix-Loop-Helix Motifs; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins