Viability and function of 8-day-stored apheresis platelets.
BACKGROUND
Methods of bacterial detection and pathogen inactivation of platelets (PLTs) may allow extended storage of PLTs as long as PLT quality is maintained.


METHODS
Twenty normal volunteers had their PLTs collected with an apheresis machine (Haemonetics Corp.). A variety of in vitro PLT function and metabolic assays were performed both on Day 0 and after 8 days of storage. On Day 8, a small blood sample was drawn from each donor to obtain fresh PLTs. The fresh and stored autologous PLTs were labeled with either (51)Cr or (111)In, and the radiolabeled PLTs were transfused. Posttransfusion serial blood samples were drawn to determine the relative posttransfusion recoveries and survivals of the fresh versus the stored PLTs.


RESULTS
Although the in vitro assays showed some differences between the two trial sites, the results were generally within the ranges expected for fresh and stored PLTs. Overall, PLT recoveries averaged 66 +/- 16 percent versus 53 +/- 20 percent and survivals averaged 8.5 +/- 1.6 days versus 5.6 +/- 1.6 days, respectively, for fresh compared to 8-day-stored PLTs. There were no significant differences in the in vivo PLT data between the trial sites or based on the radiolabel used for the measurements.


CONCLUSIONS
After 8 days of storage, the in vivo posttransfusion recovery and survival of autologous Haemonetics apheresis PLTs meet the proposed standards for poststorage PLT quality.
Slichter
Sherrill J
SJ
Puget Sound Blood Center, Seattle, Washington 98104-1256, USA. sjslichter@psbc.org
Bolgiano
Doug
D
Jones
Mary Kay
MK
Christoffel
Todd
T
Corson
Jill
J
Rose
Leslie
L
Foley
Jim
J
Popovsky
Mark
M
Baril
Laurene L
LL
Corda
Tammy
T
Dincecco
Dorothy M
DM
Snyder
Edward L
EL
D001792:Blood Platelets / Q000166:cytology; D001793:Blood Preservation / Q000379:methods / Q000592:standards; D001804:Blood Transfusion, Autologous / Q000379:methods / Q000592:standards; D005260:Female; D006801:Humans; D008297:Male; D017713:Platelet Transfusion / Q000379:methods / Q000592:standards; D010983:Plateletpheresis / Q000379:methods / Q000592:standards; D013997:Time Factors