BRX mediates feedback between brassinosteroid levels and auxin signalling in root growth.
Brassinosteroid and auxin decisively influence plant development, and overlapping transcriptional responses to these phytohormones suggest an interaction between the two pathways. However, whether this reflects direct feedback or merely parallel inputs on common targets is unclear. Here we show that in Arabidopsis roots, this interaction is mediated by BREVIS RADIX (BRX), which is required for optimal root growth. We demonstrate that the brx phenotype results from a root-specific deficiency of brassinosteroid and is due to reduced, BRX-dependent expression of a rate-limiting enzyme in brassinosteroid biosynthesis. Unexpectedly, this deficiency affects the root expression level of approximately 15% of all Arabidopsis genes, but the transcriptome profile can be restored to wild type by brassinosteroid treatment. Thus, proper brassinosteroid levels are required for the correct expression of many more genes than previously suspected. Moreover, embryonic or post-embryonic brassinosteroid application fully or partially, respectively, rescues the brx phenotype. Further, auxin-responsive gene expression is globally impaired in brx, demonstrating that brassinosteroid levels are rate-limiting for auxin-responsive transcription. BRX expression is strongly induced by auxin and mildly repressed by brassinolide, which means that BRX acts at the nexus of a feedback loop that maintains threshold brassinosteroid levels to permit optimal auxin action.
Mouchel
Céline F
CF
Department of Plant Molecular Biology, Biophore Building, University of Lausanne, CH-1015 Lausanne, Switzerland.
Osmont
Karen S
KS
Hardtke
Christian S
CS
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D060406:Brassinosteroids; D002777:Cholestanols / Q000378:metabolism / Q000494:pharmacology; D025461:Feedback, Physiological; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005816:Genetic Complementation Test; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D010937:Plant Growth Regulators / Q000378:metabolism* / Q000494:pharmacology; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013260:Steroids, Heterocyclic / Q000378:metabolism / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
D029681:Arabidopsis Proteins; C483078:BREVIS RADIX protein, Arabidopsis; D060406:Brassinosteroids; D002777:Cholestanols; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D013260:Steroids, Heterocyclic; C023623:brassinolide