[Determination of capsaicin in Pheretima aspergillum from different habitats by RP-HPLC].
OBJECTIVE
To determine the content of hypoxanthine in Pheretima aspergillum from different habitats.


METHODS
A RP-HPLC method was established. The chromatographic column was Inertsil ODS-EP. The mobile phase was H2O-CH3OH-C4H8O(93:: 7: 0.05). The flow rate was 1.0 ml/min, and the detection wavelength was 254 nm.


RESULTS
The average recoveries for hypoxanthine was 98.6% , precision of the method was 0. 50% (RSD, n = 6).


CONCLUSIONS
The method can be used to determine the content of hypoxanthine in Pheretima aspergillum from diffrent habitats.
Li
Jian
J
Department of Pharmaceutics, Guangdong College of Pharmacy, Guangzhou 510006, China.
Long
Xiao-ying
XY
He
Lin
L
Liu
Fang
F
Shu
Jie-qian
JQ
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019271:Hypoxanthine / Q000032:analysis*; D008419:Materia Medica / Q000032:analysis / Q000737:chemistry*; D009835:Oligochaeta / Q000737:chemistry*; D010598:Pharmacognosy; D011786:Quality Control
D008419:Materia Medica; D019271:Hypoxanthine