title: Thin layer chromatographic analysis of glucose and maltose in estivated Biomphalaria glabrata snails and those infected with Schistosoma mansoni.
abstract: Thin layer chromatography was used to analyze the glucose and maltose concentrations of the digestive gland-gonad complex (DGG) of uninfected-estivated Biomphalaria glabrata snails and estivated B. glabrata patently infected with Schistosoma mansoni. All snails were estivated in a most chamber at a relative humidity of 98+/-1% and a temperature of 23+/-1 degrees C for 14 days. Carbohydrates were extracted from the DGG with 70% aqueous ethanol, and extracts were analyzed on silica gel preadsorbent plates using ethyl acetate-glacial acetic acid-methanol-water (60:15:15:10) mobile phase, alpha-naphthol-sulfuric acid detection reagent, and quantification by densitometry. The concentrations of glucose and maltose were significantly reduced in both uninfected-estivated snails and infected-estivated snails.
authors.affiliation: Department of Chemistry, Lafayette College, Easton, PA 18042, USA.
authors.forename: Jamie A
authors.initials: JA
authors.lastname: Jarusiewicz
authors.forename: Joseph
authors.initials: J
authors.lastname: Sherma
authors.forename: Bernard
authors.initials: B
authors.lastname: Fried
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000737:chemistry / Q000469:parasitology* / Q000502:physiology*; D002855:Chromatography, Thin Layer / Q000379:methods; D004032:Diet; D004064:Digestive System / Q000737:chemistry; D004956:Estivation; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D006790:Host-Parasite Interactions; D008320:Maltose / Q000032:analysis* / Q000378:metabolism; D012550:Schistosoma mansoni / Q000254:growth & development*; D012680:Sensitivity and Specificity
chemical_list: D008320:Maltose; D005947:Glucose