title: The use of the DR CALUX bioassay and indicator polychlorinated biphenyls for screening of elevated levels of dioxins and dioxin-like polychlorinated biphenyls in eel.
abstract: The DR CALUX bioassay is a very suitable screening method for dioxins and dioxin-like-PCBs in feed and food. This was, e. g. demonstrated in a survey in the Netherlands to control the dioxin levels in eel. The DR CALUX assay, but also indicator polychlorinated biphenyls (PCB) were evaluated as a screening method. Based on the limit for polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/F) [at that time 8 pg toxic equivalents (TEQ)/g eel], and the relation between PCDD/F and dioxin-like-PCB, a decision limit of 30 pg TEQ/g eel was used for screening of 153 field samples. Suspected samples (21) and part of the higher contaminated negative samples (35) were analyzed by GC/MS for dioxins, non-ortho, mono-ortho and indicator PCB, revealing 13 samples exceeding the action limit of 30 pg TEQ/g eel. Only one sample slightly exceeded the dioxin level of 8 pg TEQ/g eel. The relatively low sensitivity for mono-ortho PCB was overcome by the use of reference samples, as shown by the correlation of 0.93 between GC/MS and CALUX determined total TEQ levels. The present data show that the DR CALUX assay can be used for screening of total TEQ levels in eel. The use for dioxins only requires a safe, and therefore relatively low, decision limit. The indicator PCB also showed a good correlation with total TEQ levels, mainly due to the large contribution of the mono-ortho PCB at higher concentrations. The relation with dioxins was very poor and as such indicator PCB seem less suitable than the DR CALUX assay for screening for dioxins only. The present study clearly shows that part of the wild eel samples contains high total TEQ levels and will exceed the future European Union limit of 12 pg TEQ/g eel for dioxins and dioxin-like PCB. Especially at high TEQ levels, dioxin-like PCB contribute most to the total TEQ. In practice, wild eel presents only a minor part of the eel consumed.
authors.affiliation: RIKILT, Institute of Food Safety, Wageningen, The Netherlands. ron.hoogenboom@wur.nl
authors.forename: Ron
authors.initials: R
authors.lastname: Hoogenboom
authors.forename: Toine
authors.initials: T
authors.lastname: Bovee
authors.forename: Win
authors.initials: W
authors.lastname: Traag
authors.forename: Ronald
authors.initials: R
authors.lastname: Hoogerbrugge
authors.forename: Bert
authors.initials: B
authors.lastname: Baumann
authors.forename: Liza
authors.initials: L
authors.lastname: Portier
authors.forename: Guido
authors.initials: G
authors.lastname: van de Weg
authors.forename: Jaap
authors.initials: J
authors.lastname: de Vries
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D001681:Biological Assay / Q000379:methods; D045744:Cell Line, Tumor; D004147:Dioxins / Q000032:analysis* / Q000494:pharmacology; D004524:Eels; D005506:Food Contamination / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D015870:Gene Expression / Q000187:drug effects; D008156:Luciferases / Q000235:genetics; D051379:Mice; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000494:pharmacology; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014162:Transfection
chemical_list: D004147:Dioxins; D011078:Polychlorinated Biphenyls; D008156:Luciferases