Complete NMR characterization of lychnose from Stellaria media (L.) Vill.
Lychnose (alpha-D-Gal-(1-->6)-alpha-D-Glc-(1-->2)-beta-D-Fru-(1-->1)-alpha-D-Gal) was isolated from Stellaria media, a representative member of the Caryophyllaceae plant family. Weak acid hydrolysis, enzymatic hydrolysis and complete NMR characterization were performed to confirm the identity of the tetrasaccharide. All (1)H and (13)C resonances were unambiguously assigned and the conformation of the sugars was determined using one and two dimensional NMR techniques. Anomeric characterizations in lychnose were confirmed from HMBC and NOESY spectra.
Vanhaecke
Mieke
M
Laboratory of Molecular Plant Physiology, Institute of Botany and Microbiology, K.U. Leuven, Kasteelpark Arenberg 31, B-3001 Heverlee, Belgium.
Van den Ende
Wim
W
Van Laere
André
A
Herdewijn
Piet
P
Lescrinier
Eveline
E
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry; D029752:Stellaria / Q000737:chemistry*
D009844:Oligosaccharides; C515781:galactopyranoyl-1-6-glucopyranosyl-1-2-fructofuranosyl-1-1-galactopyranoside