[Assessment of malonyldialdehyde concentration as a product of lipid peroxidation and lipid metabolism in patients on chronic dialysis].
Chronic hemodialysis in patients with renal insufficiency is a factor significantly intensifying oxidative stress, resulting in increased lipid and lipoprotein peroxidation. It intensifies atherosclerotic activity. Malonyldialdehyde (MDA) concentration in serum, and isolated fraction of low density lipoprotein is indicators of lipid peroxidation intensification and therefore arteriosclerosis development.


OBJECTIVE
Assessment of MDA concentration as a product of lipid peroxidation and lipid metabolism in patients on chronic dialysis.


METHODS
The study included 48 dialyzed patients, age 22-68 undergoing chronic dialysis for, on average, 56 months. On account of dialysis period the patients were included into 3 subgroups: group 1 (n = 17) dialysis period 2 years, group 2 (n = 13) dialysis period 2-5 years and group 3 (n = 18) dialysis period longer than 5 years. Control group consisted of 30 healthy subjects. We assessed: malonyldialdehyde in serum and isolated fraction of low density lipids (LDL) and high density lipid (HDL) and cholesterol (CH) and triglycerides (TG), phospholipids (PL) in serum and isolated lipoprotein fractions.


RESULTS
Significantly decreased levels of HDL, LDL fractions and increased triglicerides and elevated levels of MDA in serum and isolated LDL fraction were found in patients on chronic dialysis in comparison to control group (p < 0.05).


CONCLUSIONS
Our results indicate apparent normalization of lipid metabolism in patients on chronic dialysis and suggest that MDA assessment is better marker for arteriosclerosis risk estimation in long-term dialyzed patients.
Miler
Marlena
M
Uniwersytet Medyczny w Lodzi, Zakład Diagnostyki Laboratoryjnej, Katedry Diagnostyki Laboratoryjnej. mmiler@plusnet.pl
Zamojska
Sylwia
S
Fijałkowski
Paweł
P
Lobos
Marek
M
Paradowski
Marek T
MT
D000328:Adult; D000368:Aged; D008078:Cholesterol, LDL / Q000097:blood*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy*; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood*; D006435:Renal Dialysis / Q000379:methods*; D014280:Triglycerides / Q000097:blood*
D008078:Cholesterol, LDL; D010743:Phospholipids; D014280:Triglycerides; D008315:Malondialdehyde