title: Detection of heterozygous nonsense mutations in genes of interest using an Escherichia coli-based stop codon assay.
abstract: Since nonsense mutations are closely associated with severe conditions of genetic disorders, including familial cancers, rapid and precise detection of those mutations is very important for research purposes and molecular diagnosis. Currently, screening methods such as the FASAY (functional analysis of separated alleles in yeast) and the Y-SC (stop codon assay in yeast) are used for functional detection of nonsense mutations in genes of interest. But these yeast-based approaches are time-consuming, expensive and complicated. In order to circumvent these problems, we, in the present study, devised a novel Escherichia coli-based screening method, the E-SC (E. coli stop codon assay) for the detection of heterozygous nonsense mutations in genes of interest. Our strategy was based on the fact that the plasmid replicating with a low copy number in E. coli allows an effective separation of normal and mutant alleles. Moreover, it relies on the expression vector, resulting in the formation of white and blue colonies for mutant and normal alleles through the expression of PCR-amplified fragment/lacZ fusion protein respectively. To evaluate the applicability of the E-SC method for the detection of the heterozygous truncating mutation, PCR-amplified exon 7 of the StAR [steroidogenic acute regulatory protein; causative gene of the CAH (congenital lipoid adrenal hyperplasia)] and RT (reverse transcription)-PCR-amplified full-length cDNA of MeCP2 (methyl-CpG-binding protein 2; causative gene of Rett syndrome) were used. The E-SC showed an almost 1:1 ratio of blue/white colonies in all patients examined, whereas the control samples produced blue colonies only. These results demonstrate that the E-SC system is useful for rapid and precise detection of known and unknown heterozygous truncation mutations in genes that cause genetic disorders and familial cancers.
authors.affiliation: Department of Life Science, College of Natural Science, Chung-Ang University, Heuk suk dong, Dongjak gu, Seoul, Republic of Korea.
authors.forename: Young Joon
authors.initials: YJ
authors.lastname: Moon
authors.forename: Yoonsung
authors.initials: Y
authors.lastname: Kang
authors.forename: Jee-Hye
authors.initials: JH
authors.lastname: Choi
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000312:Adrenal Hyperplasia, Congenital / Q000235:genetics*; D001681:Biological Assay / Q000379:methods*; D018389:Codon, Nonsense / Q000235:genetics*; D018388:Codon, Terminator / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D010750:Phosphoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D018389:Codon, Nonsense; D018388:Codon, Terminator; D010750:Phosphoproteins; D000098682:Steroidogenic Acute Regulatory Protein