Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells.
To exclude bacteria- or animal-derived factors from cultured fabrication of transplantable epithelial cell sheets, primary human oral mucosal epithelial cells were seeded on temperature-responsive culture inserts having submicron-scale pores. Supplying culture medium containing human autologous serum to both apical and basal sides of human epithelial cells allows these cells to grow to confluence. These proliferating cells created stratified epithelial layers even when 3T3 feeder layers and fetal bovine serum were eliminated from culture. Normal keratin expression profiles were obtained with these cells, and basal and midlayer cells expressed p63, a putative stem/progenitor marker. These results suggest that temperature-responsive culture inserts can be useful in clinical settings that require the exclusion of xenogeneic factors.
Murakami
Daisuke
D
Graduate School of Science and Engineering, Waseda University, Tokyo, Japan.
Yamato
Masayuki
M
Nishida
Kohji
K
Ohki
Takeshi
T
Takagi
Ryo
R
Yang
Joseph
J
Namiki
Hideo
H
Okano
Teruo
T
D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media; D004285:Dogs; D004847:Epithelial Cells / Q000637:transplantation* / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009061:Mouth Mucosa / Q000166:cytology*; D033581:Stem Cell Transplantation / Q000379:methods; D013696:Temperature; D019172:Transplantation Conditioning / Q000379:methods*
D003470:Culture Media