New screening method for basic compounds in urine by on-line extraction-high-performance liquid chromatography with photodiode-array detection.
A fully automated, qualitative screening HPLC method for the identification of basic compounds in urine has been developed. A 1-ml volume of urine was extracted by on-line extraction and separated on two coupled strong cation-exchange (SCX) columns (2 x LunaSCX, 150 mm x 4.6 mm, 5 microm) under isocratic conditions. The mobile phase consisted of a mixture of potassium dihydrogenphosphate buffer (pH 2.3) and acetonitrile. The use of photodiode-array detection (DAD, lambda = 190-800 nm) gave access to a library of approximately 2600 toxicologically relevant compounds. The validated method is reliable, simple and in addition successfully proven with the analysis of real biological specimen for the routine use.
Schönberg
Lena
L
Berliner Betrieb fuer Zentrale Gesundheitliche Aufgaben (BBGes), Institute of Clinical Toxicology-Clinical Toxicology and Poison Control Center, Oranienburgerstrasse 285, 13437 Berlin, Germany.
Grobosch
Thomas
T
Lampe
Dagmar
D
Kloft
Charlotte
C
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D000662:Amphetamines / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009862:Online Systems; D018847:Opioid Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D015203:Reproducibility of Results
D000470:Alkaloids; D000662:Amphetamines; D018847:Opioid Peptides