Comparison of mice born after intracytoplasmic sperm injection with in vitro fertilization and natural mating.
The procedures of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are routinely used in modern medicine to overcome infertility and, in animal husbandry, to propagate lines with compromised fertility. However, there remains concern that manual selection and injection of whole sperm into oocytes could contribute to pre- and postnatal developmental defects. To address this, we have used gene expression profiling and immunophenotyping to characterize offspring generated by these procedures. We used gametes from glutathione peroxidase 1 knockout (Gpx1-/-) mice as a sensitized screen responsive to oxidative stress from artificial reproduction technologies (ART). There were no differences between IVF and ICSI derived offspring in gene expression patterns, and minor differences in hematopoietic parameters. Furthermore there were only minor differences between these IVF and ICSI pups and those derived from natural mating. These data demonstrate for the first time in that there is no significant phenotypic affects of ICSI when compared to IVF and we identified a relatively minor influence of the artificial fertilization methods on phenotype of offspring compared with natural mating. These observations would support the use of ICSI for derivation of mutant mouse lines and may be of some importance for the use of this technique in human ART.
Wilson
Trevor J
TJ
Centre for Functional Genomics and Human Disease, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia.
Lacham-Kaplan
Orly
O
Gould
Jodee
J
Holloway
Andrew
A
Bertoncello
Ivan
I
Hertzog
Paul J
PJ
Trounson
Alan
A
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology / Q000378:metabolism; D005260:Female; D005306:Fertilization / Q000502:physiology*; D005307:Fertilization in Vitro / Q000662:veterinary; D020869:Gene Expression Profiling; D005979:Glutathione Peroxidase / Q000235:genetics; D016130:Immunophenotyping; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D011247:Pregnancy; D011270:Pregnancy, Animal; D020554:Sperm Injections, Intracytoplasmic / Q000662:veterinary*; D000094765:Glutathione Peroxidase GPX1
D005979:Glutathione Peroxidase; D000094765:Glutathione Peroxidase GPX1; C000722068:Gpx1 protein, mouse