Synthesis and characterization of immunogens based on calix[4]arene-crown-6 for the generation of antibodies directed towards cesium ions.
With the aim of generating antibodies directed towards cesium, a calix[4]arene-crown-6 in the 1,3-alternate conformation bearing an N-hydroxysuccinimide function was synthesized. The calixarene was coupled to bovine serum albumin using different calixarene/protein ratios, and cesium was loaded. Conjugates were characterized using trinitrobenzenesulfonic acid derivatization, matrix assisted laser desorption ionisation-mass spectrometry (MALDI-TOF-MS), and inductively coupled plasma-mass spectrometry (ICP-MS). Targeted lysines were determined after enzymatic cleavage followed by MALDI-TOF-MS, and a progressive targeting related to lysines accessibility was observed.
Safi
Samir
S
Laboratoire de Chimie Analytique et Minérale, UMR 7178 ULP/CNRS/IN2P3, LC4, ECPM, 25 rue Becquerel, F-67087 Strasbourg Cedex, France.
Asfari
Zouhair
Z
Ehret-Sabatier
Laurence
L
Leroy
Maurice
M
Hagège
Agnès
A
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D047250:Calixarenes / Q000737:chemistry*; D002417:Cattle; D002586:Cesium / Q000276:immunology*; D047248:Crown Compounds; D043844:Crown Ethers / Q000737:chemistry*; D008239:Lysine / Q000378:metabolism; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D014614:Vaccines, Synthetic / Q000737:chemistry* / Q000276:immunology
D000906:Antibodies; D047248:Crown Compounds; D043844:Crown Ethers; D010636:Phenols; D014614:Vaccines, Synthetic; C121325:calix(4)arene; D047250:Calixarenes; D002586:Cesium; D012710:Serum Albumin, Bovine; D008239:Lysine