title: [Study of L-02 hepatocyte apoptosis induced by hexavalent chromium associated with mitochondria function damage].
abstract: OBJECTIVE
  To explore the effect of hexavalent chromium on apoptosis of L-02 hepatocytes and the functions of mitochondria. METHODS L-02 hepatocytes in all tests were incubated with 0,2,4,8,16,32 [see text] 64 micromol/L of Cr(VI) for 6h. Apoptosis of L-02 hepatocytes in the presence of Cr(VI) was quantified by flow cytometry (FCM). The permeability transition pore (FTP) of mitochondria and mitochondrial membrane potential as indicators of mitochondrial damage were measured by fluorescent spectrometer.
  
  
  RESULTS
  Concentration-dependent decrease in cell apoptosis rate of Cr(VI)-treated L-02 hepatocytes were observed. The results of permeability transition pore (PTP) of mitochondria, mitochondrial membrane potential in all concentrations of Cr(VI) had significant difference when compared to the control cells (P < 0.05).
  
  
  CONCLUSIONS
  The results demonstrated that L-02 hepatocytes apoptosis induced by Cr(VI) associated with mitochondrial damages.
authors.affiliation: Institute for Occupational Health and Poison Control, China CDC, Beijing 100050, China.
authors.forename: Jing-wei
authors.initials: JW
authors.lastname: Xiao
authors.forename: Cai-gao
authors.initials: CG
authors.lastname: Zhong
authors.forename: Bin
authors.initials: B
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002857:Chromium / Q000633:toxicity*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000502:physiology*; D033681:Mitochondrial Membrane Transport Proteins / Q000187:drug effects*; D000083162:Mitochondrial Permeability Transition Pore
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D002857:Chromium; C074702:chromium hexavalent ion