Plasma antioxidants in subjects before hematopoietic stem cell transplantation.
Chemo-irradiation induced oxidative damage to vascular endothelium may contribute to pulmonary complications of hematopoietic stem cell transplantation (HSCT). We measured antioxidants, markers of oxidative stress and plasma antioxidant capacity in plasma or serum from 24 subjects at day 7 before HSCT and 20 control subjects. The plasma concentration of extracellular glutathione peroxidase (GPX-3) was significantly reduced in the HSCT subjects compared with controls (HSCT: 98+/-42 microg/ml, control: 169+/-56 microg/ml, P<0.0001). The concentration of gamma-tocopherol was significantly higher in the HSCT subjects compared with controls (HSCT: 207+/-103 microg/dl;


METHODS
98+/-52 microg/dl; P=0.0002). The plasma concentrations of protein carbonyl, nitrotyrosine, malondialdehyde, alpha-tocopherol, vitamin A, homocysteine, cysteine and cysteinylglycine did not differ between HSCT and control subjects. Plasma from HSCT subjects was as effective as control plasma in quenching menadione-induced intracellular reactive oxygen species production in human microvascular endothelial cells. In summary, subjects before HSCT have significantly reduced plasma concentrations of GPX-3, elevated plasma gamma-tocopherol yet retains the ability to quench an acute oxidative stress. These changes may play a role in chronic oxidative stress in the HSCT population.
White
A C
AC
Pulmonary, Critical Care and Sleep Division, Department of Medicine, Tufts-New England Medical Center, Tupper Research Institute, Tufts University School of Medicine, Boston, MA, USA. AWhite@NESinai.org
Sousa
A M
AM
Blumberg
J
J
Ryan
H F
HF
Fanburg
B L
BL
Kayyali
U S
US
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000009:adverse effects / Q000627:therapeutic use; D000975:Antioxidants / Q000032:analysis*; D015415:Biomarkers; D016009:Chi-Square Distribution; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D011832:Radiation Injuries; D017382:Reactive Oxygen Species / Q000378:metabolism; D012307:Risk Factors; D019172:Transplantation Conditioning / Q000009:adverse effects
D000975:Antioxidants; D015415:Biomarkers; D017382:Reactive Oxygen Species; C486484:GPX3 protein, human; D005979:Glutathione Peroxidase