title: A hypothesis for vulnerable plaque rupture due to stress-induced debonding around cellular microcalcifications in thin fibrous caps.
abstract: In this article, we advance a hypothesis for the rupture of thin fibrous cap atheroma, namely that minute (10-mum-diameter) cellular-level microcalcifications in the cap, which heretofore have gone undetected because they lie below the visibility of current in vivo imaging techniques, cause local stress concentrations that lead to interfacial debonding. New theoretical solutions are presented for the local stress concentration around these minute spherical inclusions that predict a nearly 2-fold increase in interfacial stress that is relatively insensitive to the location of the hypothesized microinclusions in the cap. To experimentally confirm the existence of the hypothesized cellular-level microcalcifications, we examined autopsy specimens of coronary atheromatous lesions using in vitro imaging techniques whose resolution far exceeds conventional magnetic resonance imaging, intravascular ultrasound, and optical coherence tomography approaches. These high-resolution imaging modalities, which include confocal microscopy with calcium-specific staining and micro-computed tomography imaging, provide images of cellular-level calcifications within the cap proper. As anticipated, the minute inclusions in the cap are very rare compared with the numerous calcified macrophages observed in the necrotic core. Our mathematical model predicts that inclusions located in an area of high circumferential stress (>300 kPa) in the cap can intensify this stress to nearly 600 kPa when the cap thickness is <65 microm. The most likely candidates for the inclusions are either calcified macrophages or smooth muscle cells that have undergone apoptosis.
authors.affiliation: Department of Biomedical Engineering, City College of New York, Convent Avenue and 138th Street, New York, NY 10031, USA.
authors.forename: Yuliya
authors.initials: Y
authors.lastname: Vengrenyuk
authors.forename: Stéphane
authors.initials: S
authors.lastname: Carlier
authors.forename: Savvas
authors.initials: S
authors.lastname: Xanthos
authors.forename: Luis
authors.initials: L
authors.lastname: Cardoso
authors.forename: Peter
authors.initials: P
authors.lastname: Ganatos
authors.forename: Renu
authors.initials: R
authors.lastname: Virmani
authors.forename: Shmuel
authors.initials: S
authors.lastname: Einav
authors.forename: Lane
authors.initials: L
authors.lastname: Gilchrist
authors.forename: Sheldon
authors.initials: S
authors.lastname: Weinbaum
mesh_terms: D050197:Atherosclerosis / Q000473:pathology*; D002114:Calcinosis / Q000473:pathology*; D009202:Cardiomyopathies / Q000473:pathology*; D003331:Coronary Vessels / Q000473:pathology; D006801:Humans; D002479:Inclusion Bodies / Q000473:pathology; D018613:Microscopy, Confocal; D008954:Models, Biological; D012421:Rupture; D014057:Tomography, X-Ray Computed