title: [The construction and primary screening of a phage display library of HCV C and E1 genes evolved with an artificial pattern].
abstract: OBJECTIVE
  To construct and screen a primarily phage display library of HCV C and E1 genes evolved with an artificial pattern.
  
  
  METHODS
  Two genes of about 1 kb with different genotypes were evolved by DNA shuffling. The re-assembled HCV C and E1 genes were cloned into a phage vector. After being rescued with helper phage M13KO7, a phage display library was constructed. Then the library was screened with anti-C and E1 McAb. Double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was carried out on twenty individual phage clones selected randomly to detect their binding and reactive activity with high-titer HCV-positive sera. Normal sera were used as controls.
  
  
  RESULTS
  The phage display library of HCV C and E1 genes which evolved with an artificial pattern was constructed. Their capacity amounted to 1.64 x 10(6), and 86 percent of the clones contained C and E1 genes. After four rounds of panning, the phage library was specifically enriched. Twelve positive clones were successfully screened.
  
  
  CONCLUSIONS
  The capacity and diversity of the constructed library are enough for screening. The results demonstrate the superiority of the specific binding and reactive activity and affinity of the 12 phage clones from the HCV positive sera.
authors.affiliation: PLA Center of Diagnosis and Treatment for Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Fu-tao
authors.initials: FT
authors.lastname: Zhao
authors.forename: Zhan-sheng
authors.initials: ZS
authors.lastname: Jia
authors.forename: Jin-ge
authors.initials: JG
authors.lastname: Li
authors.forename: Chun-yu
authors.initials: CY
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Wei
authors.forename: Guang-yu
authors.initials: GY
authors.lastname: Li
authors.forename: Xue-fan
authors.initials: XF
authors.lastname: Bai
mesh_terms: D004279:DNA, Viral / Q000235:genetics*; D015723:Gene Library; D016174:Hepacivirus / Q000235:genetics*; D019151:Peptide Library; D014758:Viral Core Proteins / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; C087035:E1 protein, Hepatitis C virus; D019151:Peptide Library; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins