Optimization of cutaneous electrically mediated plasmid DNA delivery using novel electrode.
The easy accessibility of skin makes it an excellent target for gene transfer protocols. To take advantage of skin as a target for gene transfer, it is important to establish an efficient and reproducible delivery system. Electroporation is an established technique for enhancing plasmid delivery to many tissues in vivo. A critical component of this technique is the electrode configuration. Electroporation parameters were optimized for transgene expression with minimal tissue damage with a novel electrode. The highest transgene expression and efficiency of individual cell transformation with minimal damage was produced with eight 150 ms pulses at field strength of 100 V/cm. This electrode design offers the potential for easier and more reproducible electrically mediated cutaneous plasmid delivery than the simple electrodes currently commercially available. This electrode can be a valuable tool in determining the applicability of electrically mediated cutaneous gene transfer.
Heller
L C
LC
Center for Molecular Delivery, University of South Florida, Tampa, FL 33612, USA. rheller@hsc.usf.edu
Jaroszeski
M J
MJ
Coppola
D
D
McCray
A N
AN
Hickey
J
J
Heller
R
R
D000818:Animals; D004566:Electrodes; D018274:Electroporation / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005260:Female; D015870:Gene Expression; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009336:Necrosis; D010957:Plasmids / Q000008:administration & dosage*; D012867:Skin / Q000201:enzymology* / Q000473:pathology
D008156:Luciferases