Systems biology in cancer research: genomics to cellomics.
Cancers result from large-scale deregulation of genes that lead to cancer pathophysiologies such as increase proliferation, decreased apoptosis, increased motility, increased angiogenesis, and others. Genes that influence proliferation and apoptosis are particularly attractive as therapeutic targets. To identify genes that influence these phenotypes, we have developed simple and rapid methods to measure apoptosis and cell proliferation using high content screening with YO-PRO-1 and anti-BrdU staining of BrdU pulsed cells, respectively.
Stilwell
Jackie L
JL
Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Guan
Yinghui
Y
Neve
Richard M
RM
Gray
Joe W
JW
D001562:Benzimidazoles / Q000378:metabolism; D001973:Bromodeoxyuridine; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D023281:Genomics; D006801:Humans; D007091:Image Processing, Computer-Assisted; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D034741:RNA, Small Interfering / Q000378:metabolism; D013194:Staining and Labeling; D013223:Statistics as Topic; D049490:Systems Biology; D046888:Tissue Array Analysis
D001562:Benzimidazoles; D034741:RNA, Small Interfering; D001973:Bromodeoxyuridine