[Study on bisphenol A induced primary cultured mesencephalic neuronal cell injury by oxidative stress].
OBJECTIVE
To investigate the effect and mechanism of bisphenol A, a common EDCs, on the primary cultured mesencephalic neuronal cell injury.


METHODS
mesencephalic neuronal cells were obtained from embryonic 14-15 day SD fetuses and were cultured in serum free medium for 4 or 7 days in vitro, 1,10,25,50 and 100 micromol/L bisphenol A were added to the medium and intracellular ROS, SOD, MDA, GSH were measured after 24 or 48hours, respectively. Flow cytometry was used to detect the neuronal cell apoptosis. Tyrosine hydroxylase immunohistochemistry was used to identity and count the ratio of dopaminergic neuronal cell.


RESULTS
The intracellular ROS was dose dependently increased by bisphenol A in all treated groups, and it appeared to be a nonselective effect when the concentration of bisphenol A were higher than 50 micromol/L, and concomitantly decreased SOD, GSH and increased MDA. The apoptotic neuronal cells were significantly increased at 501 micromol/L and 100 micromol/L. The ratio of Tyrosine hydroxylase positive neuronal cell was dose dependently decreased with the concentration of bisphenol A.


CONCLUSIONS
ROS induced by bisphenol A may play a major role in its cytotoxicity on dopaminergic neuronal cell.
Lin
Yong
Y
Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Zeng
Xiang-gui
XG
Wu
De-sheng
DS
Wang
Xia
X
D000818:Animals; D001559:Benzhydryl Compounds; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism; D004622:Embryo, Mammalian; D005260:Female; D008636:Mesencephalon / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004298:Dopamine; D010636:Phenols; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; C006780:bisphenol A; D000098826:Bisphenol A Compounds