title: Thymidine kinase mutations conferring acyclovir resistance in herpes simplex type 1 recombinant viruses.
abstract: Contributions of thymidine kinase (TK) mutations to acyclovir (ACV) resistance were evaluated in herpes simplex virus type 1 recombinant viruses generated using a set of overlapping cosmids and plasmids. Alterations in both conserved and nonconserved regions of the TK gene were shown to confer high levels of resistance to ACV.
authors.affiliation: CHUQ-CHUL, room RC-709, 2705, blvd Laurier, Sainte-Foy, Québec, Canada.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sergerie
authors.forename: Guy
authors.initials: G
authors.lastname: Boivin
mesh_terms: D000212:Acyclovir / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D003360:Cosmids / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005838:Genotype; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D007700:Kinetics; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D013937:Thymidine Kinase; D004259:DNA-Directed DNA Polymerase; D000212:Acyclovir