title: Characterisation of Pseudomonas aeruginosa isolated from freshwater culture systems.
abstract: Members of the genus Pseudomonas are important phytopathogens and agents of human infections, while other strains and species exhibit bioremediation and biocontrol activities. Species-specific detection of Pseudomonas species in the environment may help to gain a more complete understanding of the ecological significance of these microorganisms. The objective of present study was comparative analysis of biochemically and PCR based confirmed 10 isolates of Pseudomonas aeruginosa (6 from fish intestine and 4 from pond sediment). PCR-ribotyping and PAGE revealed that there was extensive heterogeneity at the genetic and protein levels. Both genetic and phenotypic heterogeneity were more in the sediment isolates compared to the fish isolates. SDS-PAGE clearly demonstrated the differences between fish and sediment isolates as evident from the higher range of protein profiling. In antibiotic sensitivity test no habitat specific antibiogram was obtained. Zinc adversely affected the DNA of all the isolates to be amplified by PCR as DNA banding pattern was different from normal DNA in stressed DNA. Thus stress, particularly, zinc may interfere monitoring of Pseudomonas by PCR.
authors.affiliation: Microbiology Section, Central Institute of Freshwater Aquaculture, PO-Kaushalyaganga, Bhubaneswar, Orissa 751002, India.
authors.forename: S
authors.initials: S
authors.lastname: Tripathy
authors.forename: N
authors.initials: N
authors.lastname: Kumar
authors.forename: S
authors.initials: S
authors.lastname: Mohanty
authors.forename: M
authors.initials: M
authors.lastname: Samanta
authors.forename: R N
authors.initials: RN
authors.lastname: Mandal
authors.forename: N K
authors.initials: NK
authors.lastname: Maiti
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes / Q000382:microbiology; D005618:Fresh Water / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology; D007422:Intestines / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D020543:Proteome / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D021521:Ribotyping; D015032:Zinc / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D020543:Proteome; D015032:Zinc