EDTA-induced urothelial cell shedding for the treatment of superficial bladder cancer in the mouse.
OBJECTIVE
The aim of this study was to determine the effect of intravesical EDTA instillation on the development of intravesically implanted tumor cells in normal mice.


METHODS
The mouse bladder tumor (MBT-2) model was used in female C3H/eb mice to evaluate the amount of normal urothelial cell shedding, and the degree of tumor growth inhibition following intravesical EDTA instillation in comparison with phosphate-buffered saline (PBS) instillation.


RESULTS
At 1 h after instillation, the number of urothelial cells aspirated was 500-1000 per PBS-treated mouse and 10,000-20,000 per EDTA-treated mouse (P < 0.00001). The bladder weight, which reflected the effect of the agent on the tumor, was similar in the untreated and PBS-treated mice (105.46 +/- 46 mg and 106.2 +/- 50 mg, respectively). It was significantly lower in the EDTA-treated mice (80.4 +/- 42 mg) (P = 0.0045).


CONCLUSIONS
Intravesical administration of EDTA results in significant normal and neoplastic urothelial cell shedding. Intravesical irrigation with EDTA may prevent adherence of the malignant cells to the bladder wall following tumor resection.
Nativ
Ofer
O
Department of Urology, Bnay-Zion Medical Center, Haifa, Israel.
Dalal
Eilata
E
Hidas
Guy
G
Aronson
Moshe
M
D000283:Administration, Intravesical; D000818:Animals; D002614:Chelating Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D018450:Disease Progression; D004492:Edetic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D016896:Treatment Outcome; D001743:Urinary Bladder / Q000187:drug effects / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D019459:Urothelium / Q000187:drug effects / Q000473:pathology
D002614:Chelating Agents; D004492:Edetic Acid