title: Mild hydrolysis of nitriles by Fusarium solani strain O1.
abstract: High levels of an aromatic nitrilase (about 37 microkat/L culture) were induced in Fusarium solani O1 after transfer of the mycelium from a rich medium into a medium with 20 mmol/L picolinonitrile. The mycelium was entrapped in lense-shaped particles consisting of a polyvinyl alcohol/polyethylene glycol copolymer (LentiKats). The cell-free extract was immobilized by hydrophobic binding onto a Butyl Sepharose column. The enzyme was useful for the mild hydrolysis of nicotinonitrile, isonicotinonitrile and benzonitrile.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czechia.
authors.forename: V
authors.initials: V
authors.lastname: Vejvoda
authors.forename: O
authors.initials: O
authors.lastname: Kaplan
authors.forename: J
authors.initials: J
authors.lastname: Klozová
authors.forename: J
authors.initials: J
authors.lastname: Masák
authors.forename: A
authors.initials: A
authors.lastname: Cejková
authors.forename: V
authors.initials: V
authors.lastname: Jirků
authors.forename: R
authors.initials: R
authors.lastname: Stloukal
authors.forename: L
authors.initials: L
authors.lastname: Martínková
mesh_terms: D000619:Aminohydrolases / Q000096:biosynthesis*; D018914:Cells, Immobilized / Q000378:metabolism / Q000382:microbiology; D005670:Fusarium / Q000201:enzymology* / Q000254:growth & development; D006868:Hydrolysis; D009570:Nitriles / Q000378:metabolism*
chemical_list: D009570:Nitriles; D000619:Aminohydrolases; C021867:nitrilase