Double-quantum filtered rotational-echo double resonance.
The homonuclear scalar coupling of a directly bonded 13C-13C pair has been used to create a double-quantum filter (DQF) to remove the natural-abundance 13C background in 13C{15N} rotational-echo double-resonance (REDOR) experiments. The DQF scalar and REDOR dipolar evolution periods are coincident which is important for sensitivity in the event of weak 13C-15N dipolar coupling. Calculated and observed 13C{15N} DQF-REDOR dephasings were in agreement for a test sample of mixed recrystallized labeled alanines. Glycine metabolism in a single uniform-15N soybean leaf labeled for 6 min by 13CO2 was measured quantitatively by 13C{15N} DQF-REDOR with no background interferences.
Matsuoka
Shigeru
S
Department of Chemistry, Washington University, St. Louis, MO 63130, USA.
Schaefer
Jacob
J
D000465:Algorithms; D003198:Computer Simulation; D005998:Glycine / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D018515:Plant Leaves / Q000378:metabolism*; D011789:Quantum Theory; D012399:Rotation; D013025:Glycine max / Q000378:metabolism*; D013113:Spin Labels
D013113:Spin Labels; D005998:Glycine