Measurement of the adhesive force between a single Klebsiella pneumoniae type 3 fimbria and collagen IV using optical tweezers.
Type 3 fimbriae are important adhesive filaments that assist Klebsiella pneumoniae to establish an infection. Different MrkD adhesin variants on the fimbriae are known to display distinct adherence capability for the bacteria to bind extracellular matrix proteins, although the difference has not been determined physically. For this reason, the adhesive force between type 3 fimbriae and collagen IV were measured using optical tweezers. The measured force data displayed a periodic histogram thus Fourier analysis was applied to group it to extract the adhesive force of a single molecular pair. Specifically, we showed that grouping should begin with an offset at the first half of the period. Finally, we first present the adhesive force between each mrkD(V2)-, mrkD(V3)-, and mrkD(V4)-expressed fimbriae and collagen IV is 2.03, 3.79, and 2.87 pN, respectively. This result can be referred to further research on mrkD allelic effect on bacteria infection.
Chang
Bo-Jui
BJ
Department of Photonics and Institute of Electro-Optical Engineering, National Chiao Tung University, Hsinchu City 300, Taiwan.
Huang
Ying-Jung
YJ
Chan
Chia-Han
CH
Hsu
Long
L
Peng
Hwei-Ling
HL
Chang
Hwan-You
HY
Yew
Tri-Rung
TR
Liu
Cheng-Hsien
CH
Chi
Sien
S
D000268:Adhesiveness; D001422:Bacterial Adhesion; D024141:Collagen Type IV / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000502:physiology*
D024141:Collagen Type IV