Can Vero cell co-culture improve in-vitro maturation of bovine oocytes?
This study was carried out to evaluate the effect of Vero cell co-culture on developmental competence of immature oocytes. Bovine cumulus-oocyte complexes (COC) were matured in presence or absence of Vero cells. Matured oocytes were inseminated and cultured for up to 9 days. Cleavage percentages were recorded on day 2 after insemination and embryos were evaluated on a daily basis. Expanding/expanded and hatching/hatched blastocysts were used for cell number assay. Results indicated a significantly greater cleavage percentage in oocytes matured in presence of Vero cells than control (86% versus 76%, P < or = 0.05). The percentages of advanced embryos appear to be greater on a daily basis in COC matured in presence of Vero cells compared with control. However, these differences were not significant. Blastocysts derived from COC matured in the presence of Vero cells had a significantly higher (P < or = 0.05) number of inner cell mass, trophectoderm and total cell number in expanding/expanded (65.25, 224.5 and 289.7 respectively) and hatching/hatched (67.75, 289.75 and 357.5) embryos in comparison to the control (42, 203.5, 245.5 and 51.3, 265, 316.3 respectively). Results confirm that co-culture of bovine COC during in-vitro maturation, enhances their ability for cleavage and for producing blastocysts with higher quality.
Moulavi
Fariba
F
Royan Institute, Tehran, Iran.
Hosseini
Sayyed Mortaza
SM
Ashtiani
Saeid Kazemi
SK
Shahverdi
Abdolhossein
A
Nasr-Esfahani
Mohammad Hossein
MH
D000818:Animals; D002417:Cattle; D002522:Chlorocebus aethiops; D018920:Coculture Techniques / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D014709:Vero Cells / Q000166:cytology*