title: Nanoparticle probes with surface enhanced Raman spectroscopic tags for cellular cancer targeting.
abstract: We have developed biocompatible, photostable, and multiplexing-compatible surface-enhanced Raman spectroscopic tagging material (SERS dots) composed of silver nanoparticle-embedded silica spheres and organic Raman labels for cellular cancer targeting in living cells. SERS dots showed linear dependency of Raman signatures on their different amounts, allowing their possibility for the quantification of targets. In addition, the antibody-conjugated SERS dots were successfully applied to the targeting of HER2 and CD10 on cellular membranes and exhibited good specificity. SERS dots demonstrate the potential for high-throughput screening of biomolecules using vibrational information.
authors.affiliation: School of Chemical and Biological Engineering, College of Veterinary Medicine, Department of Chemistry Education, Seoul National University, Seoul 151-747, Korea.
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Jun-Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: Heejeong
authors.initials: H
authors.lastname: Choi
authors.forename: Sang-Myung
authors.initials: SM
authors.lastname: Lee
authors.forename: Bong-Hyun
authors.initials: BH
authors.lastname: Jun
authors.forename: Kyeong-Nam
authors.initials: KN
authors.lastname: Yu
authors.forename: Eunye
authors.initials: E
authors.lastname: Kuk
authors.forename: Yong-Kweon
authors.initials: YK
authors.lastname: Kim
authors.forename: Dae Hong
authors.initials: DH
authors.lastname: Jeong
authors.forename: Myung-Haing
authors.initials: MH
authors.lastname: Cho
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
mesh_terms: D006801:Humans; D053768:Metal Nanoparticles; D009369:Neoplasms / Q000473:pathology*; D013059:Spectrum Analysis, Raman / Q000379:methods*