title: The RCK domain of the KtrAB K+ transporter: multiple conformations of an octameric ring.
abstract: The KtrAB ion transporter is a complex of the KtrB membrane protein and KtrA, an RCK domain. RCK domains regulate eukaryotic and prokaryotic membrane proteins involved in K(+) transport. Conflicting functional models have proposed two different oligomeric arrangements for RCK domains, tetramer versus octamer. Our results for the KtrAB RCK domain clearly show an octamer in solution and in the crystal. We determined the structure of this protein in three different octameric ring conformations that resemble the RCK-domain octamer observed in the MthK potassium channel but show striking differences in size and symmetry. We present experimental evidence for the association between one RCK octameric ring and two KtrB membrane proteins. These results provide insights into the quaternary organization of the KtrAB transporter and its mechanism of activation and show that the RCK-domain octameric ring model is generally applicable to other ion-transport systems.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06520, USA.
authors.forename: Ronald A
authors.initials: RA
authors.lastname: Albright
authors.forename: José-Luís Vazquez
authors.initials: JL
authors.lastname: Ibar
authors.forename: Chae Un
authors.initials: CU
authors.lastname: Kim
authors.forename: Sol M
authors.initials: SM
authors.lastname: Gruner
authors.forename: João Henrique
authors.initials: JH
authors.lastname: Morais-Cabral
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry*; D027682:Cation Transport Proteins / Q000737:chemistry*; D002462:Cell Membrane / Q000378:metabolism*; D018360:Crystallography, X-Ray; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D011188:Potassium / Q000378:metabolism*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D027682:Cation Transport Proteins; C113206:KtrB protein, Bacteria; D008565:Membrane Proteins; D011188:Potassium