Prothymosin alpha interacts with free core histones in the nucleus of dividing cells.
The acidic protein prothymosin alpha (ProTalpha), with a broad presence in mammalian cells, has been widely considered to have a role in cell division, through an unrevealed mechanism in which histones may be involved in view of their ability to interact with ProTalpha in vitro. Results of co-immunoprecipitation experiments presented here demonstrate that ProTalpha interacts in vivo with core histones in proliferating B-lymphocytes (NC-37 cells). This interaction occurs with histones H3, H2A, H2B and H4 located free in the nucleoplasm, whereas no interaction was detected with histone H1, mono-nucleosome particles or chromatin. Moreover, the core histones form part of a nuclear multiprotein complex of about 700 kDa separated by ProTalpha-Sepharose affinity, with components including H3 and H4 acetyltranferases, H3 methyltransferases, hnRNP isotypes A3, A2/B1 and R, ATP-dependent and independent DNA helicases II, beta-actin and vimentin, all co-purifying by gel filtration. This indicates that the interaction of ProTalpha with core histones in the nucleus may be related to the structural modification of histones H3 and H4, and hence to chromatin activity, raising the possibility that the other proteins in the nuclear complex may play a role in this process.
Covelo
Guillermo
G
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Sarandeses
Concepción S
CS
Díaz-Jullien
Cristina
C
Freire
Manuel
M
D001402:B-Lymphocytes / Q000166:cytology; D002455:Cell Division / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology*; D002846:Chromatography, Affinity; D003600:Cytosol / Q000378:metabolism; D051548:Histone Acetyltransferases / Q000378:metabolism; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism; D006657:Histones / Q000502:physiology*; D006801:Humans; D047468:Immunoprecipitation; D009687:Nuclear Proteins / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism; D011496:Protein Methyltransferases; D011498:Protein Precursors / Q000502:physiology*; D013947:Thymosin / Q000031:analogs & derivatives* / Q000502:physiology
D006657:Histones; D009687:Nuclear Proteins; D009707:Nucleosomes; D011498:Protein Precursors; C040782:prothymosin alpha; D013947:Thymosin; D000076983:Histone Methyltransferases; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; D051548:Histone Acetyltransferases