title: Antimicrobial activity of cathelicidins BMAP28, SMAP28, SMAP29, and PMAP23 against Pasteurella multocida is more broad-spectrum than host species specific.
abstract: The antimicrobial activity of linear, cationic alpha-helical peptides from cattle (BMAP28), sheep (SMAP28 and SMAP29), and pigs (PMAP23) were assessed to determine if activity was selective for Pasteurella multocida from a particular animal species or broad-spectrum against all P. multocida tested. The antimicrobial activities of synthetic peptides were determined for P. multocida isolated from cattle (10 isolates), sheep (10 isolates), and pigs (10 isolates) in a broth microdilution assay. All thirty isolates of P. multocida were susceptible to BMAP28 (MICs and MBCs, 1.0-1.9 microM); SMAP28 and SMAP29 (MICs and MBCs, 0.2-0.7 microM); and PMAP23 (MICs and MBCs, 4.3 to > or = 6.8 microM). Overall, the results of this study suggest that synthesized cathelicidins from cattle, sheep, and pigs had broad-spectrum antimicrobial activity against all P. multocida.
authors.affiliation: Department of Periodontics, Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA. kim-brogden@uiowa.edu
authors.forename: Kim A
authors.initials: KA
authors.lastname: Brogden
authors.forename: Gwen
authors.initials: G
authors.lastname: Nordholm
authors.forename: Mark
authors.initials: M
authors.lastname: Ackermann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D001798:Blood Proteins / Q000494:pharmacology; D002353:Carrier State / Q000382:microbiology / Q000662:veterinary*; D054804:Cathelicidins; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000187:drug effects*; D011506:Proteins / Q000494:pharmacology; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; C102360:BMAP-28; D001798:Blood Proteins; D054804:Cathelicidins; D011506:Proteins; C403305:SMAP29 protein, Ovis aries; C086301:porcine myeloid antibacterial peptide 23