title: Immunogenicity of synthetic saccharide fragments of Vibrio cholerae O1 (Ogawa and Inaba) bound to Exotoxin A.
abstract: Recombinant exotoxin A (rEPA) from Pseudomonas aeruginosa conjugated to Vibrio cholerae O1 serotype-specific polysaccharides (mono-, di- and hexasaccharide) were immunogenic in mice. Monosaccharide conjugates boosted the humoral responses to the hexasaccharide conjugates. Prior exposure to purified Ogawa lipopolysaccharide (LPS) enabled contra-serotype hexasaccharide conjugates to boost the vibriocidal response, but Inaba LPS did not prime for an enhanced vibriocidal response by a contra-serotype conjugate. Prior exposure to the carrier, and priming B cells with the LPS of either serotype, resulted in enhanced vibriocidal titers if the Ogawa hexasaccharides were used, but a diminished response to the Inaba LPS. These studies demonstrate that the 'functional' B cell epitopes on the LPS differ from those of the neoglycoconjugates and that the order of immunization and the serotype of the boosting conjugate can influence the epitope specificity and function of the antisera.
authors.affiliation: Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, NH 03756, USA. william.wade@dartmouth.edu
authors.forename: Terri K
authors.initials: TK
authors.lastname: Wade
authors.forename: Rina
authors.initials: R
authors.lastname: Saksena
authors.forename: Joseph
authors.initials: J
authors.lastname: Shiloach
authors.forename: Pavol
authors.initials: P
authors.lastname: Kovác
authors.forename: William F
authors.initials: WF
authors.lastname: Wade
mesh_terms: D036002:ADP Ribose Transferases / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002771:Cholera / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control / Q000821:virology; D022121:Cholera Vaccines / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D005098:Exotoxins / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D005260:Female; D018796:Immunoconjugates / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D007075:Immunoglobulin M / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology*; D041241:Vibrio cholerae O1 / Q000737:chemistry / Q000276:immunology*; D037521:Virulence Factors / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D022121:Cholera Vaccines; D005098:Exotoxins; D018796:Immunoconjugates; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A