Increased matrix metalloproteinase-3 mRNA expression in visceral fat in mice implanted with cultured preadipocytes.
Using preadipocyte implantation methods, we recently demonstrated that adipocytes in the visceral area change their function, as the expression of tumor necrosis factor-alpha (TNF-alpha) increases, thereby causing insulin resistance. In order to clarify the mechanism for changes in the function of adipocytes in visceral area, we examined the mRNA expression profiles in visceral fat tissue specimens. Four weeks after cell implantation, we performed a microarray analysis using the RNA of fat tissue specimens implanted either with 3T3-L1 cells or PBS alone. Sixty-three genes were thus isolated and the expression of matrix metalloproteinase-3 (MMP-3) mRNA was found to dramatically increase in the fat tissue specimens. The neutralization of MMP-3 protein inhibited adipogenesis and the free fatty acid-induced TNF-alpha secretion in 3T3-L1 adipocytes. These results suggest a potential role of MMP-3, which promotes the TNF-alpha secretion, thus contributing to the disturbance of the functions in the adipocytes which accumulate in the visceral area.
Unoki
Hiroyuki
H
Division of Applied Translational Research, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
Bujo
Hideaki
H
Shibasaki
Manabu
M
Saito
Yasushi
Y
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D004195:Disease Models, Animal; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D005786:Gene Expression Regulation; D005947:Glucose / Q000494:pharmacology; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis* / Q000235:genetics; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D008819:Mice, Nude; D009765:Obesity / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis; D033581:Stem Cell Transplantation; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D005230:Fatty Acids, Nonesterified; D061965:Matrix Metalloproteinase Inhibitors; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D019278:Matrix Metalloproteinase 3; D005947:Glucose