Increased luminal mucin does not disturb glucose or ovalbumin absorption in rats fed insoluble dietary fiber.
We tested whether increased mucin secretion due to ingestion of insoluble dietary fiber (IDF) affects small intestinal nutrient absorption in rats. Polystyrene foam (PSF) with a true expansion ratio of 54.9 was used as a model for IDF with high bulk-forming properties. In Expt. 1, rats were fed a control diet or diet containing 50 g PSF/kg for 1, 3, 5, or 7 d. Small intestinal mucin fractions were isolated, and O-linked oligosaccharide chains were measured. The luminal mucin content reached a maximum within 5 d after PSF ingestion. In Expt. 2, rats were fed a control diet or diet containing 50 g PSF/kg for 7 d, and then all rats were switched to the control diet for 1, 3, or 5 d. The increased capacity for luminal mucin secretion disappeared within 5 d after ceasing PSF ingestion. In Expt. 3, rats were fed a control diet or diet containing 70 g PSF/kg for 7 d. Glucose (1g/kg) was administered orally after 12 h of food deprivation. The blood glucose concentrations did not differ between the groups. In Expt. 4, rats were fed a control diet or diet containing 90 g PSF/kg for 14 d. At d 7, portal cannulae were installed. A mixed solution of glucose (1g/kg) and ovalbumin (OVA, 250 mg/kg) was orally administered after 12 h of food deprivation, and responses of portal glucose and OVA concentrations were monitored for 120 min. Although luminal mucin contents were almost doubled in the 9% PSF group compared with the control group, neither portal glucose nor OVA concentration differed at any time point. The results suggest that the short-term ingestion of IDF significantly increases the luminal mucin content, but that this does not disturb nutrient absorption.
Morita
Tatsuya
T
Faculty of Nutritional Sciences, University of Shizuoka, Yada 52-1, Shizuoka 422-8526, Japan. actmori@agr.shizuoka.ac.jp
Tanabe
Hiroki
H
Ito
Hiroyuki
H
Yuto
Shunsuke
S
Matsubara
Takeshi
T
Matsuda
Tsukasa
T
Sugiyama
Kimio
K
Kiriyama
Shuhachi
S
D000818:Animals; D002236:Carbohydrate Conformation; D004043:Dietary Fiber / Q000008:administration & dosage*; D004591:Electrophoresis, Polyacrylamide Gel; D005508:Food Deprivation; D005947:Glucose / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005951:Glucose Tolerance Test; D007408:Intestinal Absorption / Q000502:physiology*; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D009077:Mucins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry; D010047:Ovalbumin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D011137:Polystyrenes; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012995:Solubility
D004043:Dietary Fiber; D009077:Mucins; D009844:Oligosaccharides; D011137:Polystyrenes; D010047:Ovalbumin; D005947:Glucose