[Effects of peritoneal dialysis solution on proliferation and apoptosis of rat peritoneal mesothelial cells].
OBJECTIVE
To investigate the effects of peritoneal dialysis solution (PDS) on proliferation and apoptosis of rat peritoneal mesothelial cells (RPMCs).


METHODS
The proliferation of RPMCs treated with PDS containing glucose of different concentrations for different times in vitro were examined by MTT colorimeric assay. The cell cycles and cell apoptosis rate were determined by flow cytometry.


RESULTS
After PDS treatment for 1 h, the cell proliferation inhibition rate, percentage of cells at G(0)/G(1) stage and early cell apoptosis rate increased, and such increment was more manifest with higher glucose concentration in PDS and longer treatment time of the cells. After treatment with PDS containing 4.25% glucose for 3 h, the proliferation inhibition rate of the RPMCs reached 44.12%, the percentage of cells at the G(0)/G(1) stage amounted to 71.95% and the early apoptosis rate reached 23.59%, which was several times higher than that of the negative control and 1.5% glucose/PDS groups, and also higher than that of 4.25% mannitol/PDS groups.


CONCLUSIONS
PDS containing high-concentration glucose can induce significant apoptosis and inhibit the proliferation of RPMCs in vitro.
Chen
Sheng-xiao
SX
Department of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. csx@fimmu.com
Ren
Hao
H
Liu
Zheng-rong
ZR
Liu
Hong-fa
HF
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D015314:Dialysis Solutions / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D008297:Male; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley
D015314:Dialysis Solutions