Automated cell plating and sample treatments for fixed cells in high content assays.
Robust and reliable methods for the manipulation of neural cell lines, by passaging, plating, dye labeling, imaging, fixation, and immunocytochemistry, are required to enable consistent, reproducible screens to be performed. We describe herein procedures and processes we have established to maximize the level of consistency of cell plating, fixation, and dye or antibody labeling, to ensure that assays which we are running on a routine basis remain consistent across long periods of time. These procedures involve a variety of fully or semiautomated steps, using high-quality commercially available liquid handling and dispensing technology.
Richards
Gillian R
GR
The Neuroscience Research Centre, Merck Sharp and Dohme Research Laboratories, Essex, UK.
Kerby
Julie E
JE
Chan
Grace K Y
GK
Simpson
Peter B
PB
D001322:Autoanalysis / Q000379:methods; D006801:Humans; D046888:Tissue Array Analysis / Q000379:methods*; D016707:Tissue Fixation