title: Spatial and temporal expression patterns of Avr1b-1 and defense-related genes in soybean plants upon infection with Phytophthora sojae.
abstract: The Avr1b locus is required for avirulence of the oomycete pathogen Phytophthora sojae on soybeans carrying resistance gene Rps1b. One of the Avr genes of the locus (Avr1b-1) was shown to encode an elicitor. We have analyzed the spatial and temporal expression patterns of Avr1b-1 in comparison to defense-related genes induced in soybean. Avr1b-1 expression was detectable mainly in close proximity to the site of infection, in wound-inoculated hypocotyls as well as in roots infected with zoospores. Usually, in compatible interactions, higher expression levels of Avr1b-1 were observed in roots when compared with incompatible P. sojae-soybean interactions, whereas neither the timing nor the amount of transcript accumulation of defense-related genes showed cultivar-specific differences. In contrast, the PsojNIP gene encoding a proposed virulence factor was expressed only during the necrotrophic phase in the compatible interaction.
authors.affiliation: Department Biologie I-Botanik, Ludwig-Maximilians-Universität, Menzinger Strasse 67, D-80638 Munich, Germany.
authors.forename: Karina
authors.initials: K
authors.lastname: Valer
authors.forename: Judith
authors.initials: J
authors.lastname: Fliegmann
authors.forename: Andreas
authors.initials: A
authors.lastname: Fröhlich
authors.forename: Brett M
authors.initials: BM
authors.lastname: Tyler
authors.forename: Jürgen
authors.initials: J
authors.lastname: Ebel
mesh_terms: D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D018546:Hypocotyl / Q000254:growth & development; D025282:Mycelium / Q000254:growth & development; D010838:Phytophthora / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology; D014404:Tubulin / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000199:Actins; D012333:RNA, Messenger; D014404:Tubulin