title: A gene-specific requirement for FACT during transcription is related to the chromatin organization of the transcribed region.
abstract: The FACT complex stimulates transcription elongation on nucleosomal templates. In vivo experiments also involve FACT in the reassembly of nucleosomes traversed by RNA polymerase II. Since several features of chromatin organization vary throughout the genome, we wondered whether FACT is equally required for all genes. We show in this study that the in vivo depletion of Spt16, one of the subunits of Saccharomyces cerevisiae FACT, strongly affects transcription of three genes, GAL1, PHO5, and Kluyveromyces lactis LAC4, which exhibit positioned nucleosomes at their transcribed regions. In contrast, showing a random nucleosome structure, YAT1 and Escherichia coli lacZ are only mildly influenced by Spt16 depletion. We also show that the effect of Spt16 depletion on GAL1 expression is suppressed by a histone mutation and that the insertion of a GAL1 fragment, which allows the positioning of two nucleosomes, at the 5' end of YAT1 makes the resulting transcription unit sensitive to Spt16 depletion. These results indicate that FACT requirement for transcription depends on the chromatin organization of the 5' end of the transcribed region.
authors.affiliation: Departamento de Genética, Facultad de Biología, Avda. Reina Mercedes 6, 41012-Seville, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Jimeno-González
authors.forename: Fernando
authors.initials: F
authors.lastname: Gómez-Herreros
authors.forename: Paula M
authors.initials: PM
authors.lastname: Alepuz
authors.forename: Sebastián
authors.initials: S
authors.lastname: Chávez
mesh_terms: D002843:Chromatin / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D006609:High Mobility Group Proteins / Q000235:genetics / Q000502:physiology*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D014158:Transcription, Genetic; D035602:Transcriptional Elongation Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; C073537:SSRP1 protein, human; D029701:Saccharomyces cerevisiae Proteins; D035602:Transcriptional Elongation Factors