Loss of HB-EGF in smooth muscle or endothelial cell lineages causes heart malformation.
Epidermal growth factor (EGF) and ErbB family molecules play a role in heart development and function. To investigate the role of EGF family member, heparin-binding EGF-like growth factor (HB-EGF) in heart development, smooth muscle and endothelial cell lineage-specific HB-EGF knockout mice were generated using the Cre/loxP system in combination with the SM22alpha or TIE2 promoter. HB-EGF knockout mice displayed enlarged heart valves, and over half of these mice died during the first postnatal week, while survivors showed cardiac hypertrophy. These results suggest that expression of HB-EGF in smooth muscle and/or endothelial cell lineages is essential for proper heart development and function in mice.
Nanba
Daisuke
D
Department of Biochemistry and Molecular Genetics, Ehime University Graduate School of Medicine, Shitsukawa, To-on, Ehime 791-0295, Japan.
Kinugasa
Yumi
Y
Morimoto
Chie
C
Koizumi
Michiko
M
Yamamura
Hisako
H
Takahashi
Katsuhito
K
Takakura
Nobuyuki
N
Mekada
Eisuke
E
Hashimoto
Koji
K
Higashiyama
Shigeki
S
D000818:Animals; D006332:Cardiomegaly / Q000209:etiology* / Q000473:pathology; D019070:Cell Lineage; D042783:Endothelial Cells / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005804:Genes, Lethal; D006321:Heart / Q000033:anatomy & histology / Q000254:growth & development; D006351:Heart Valves / Q000002:abnormalities* / Q000473:pathology; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D018345:Mice, Knockout; D009130:Muscle, Smooth / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism
C587884:Hbegf protein, mouse; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D004815:Epidermal Growth Factor