Immunohistochemical expression of fibronectin and tenascin after direct pulp capping with calcium hydroxide.
The aim of this study was to investigate the expression of 2 extracellullar matrix glycoproteins, fibronectin (FNC) and tenascin (TNC), following direct pulp capping with calcium hydroxide (CH). Third molars scheduled for extraction were used. Standardized class I cavities with pulp exposures were prepared. After control of bleeding, CH powder was applied in the exposure sites, which were covered with CH cement (Dycal; Dentsply) and the cavities were filled with zinc oxide-eugenol cement. Three teeth were extracted at each post-treatment period (1, 7, 14, and 30 days). Demineralized and paraffin-embedded specimens were stained for histologic technique (hematoxylin-eosin) and for immunohistochemical analysis. Anti-TNC and anti-FNC monoclonal antibodies were used with the streptavidin-biotin complex method. Generally, similar patterns of immunohistochemical expression were observed for TNC and FNC in the pulp tissue as a whole. In the exposure site, TNC immunostaining increased over time, exhibiting a thicker immunostaining pattern within 30 days. The imunohistochemical technique showed expression of both glycoproteins during pulp healing process.
Piva
Evandro
E
Department of Operative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil. piva@ufpel.edu.br
Tarquínio
Sandra B C
SB
Demarco
Flávio F
FF
Silva
Adriana F
AF
de Araújo
Vera C
VC
D002126:Calcium Hydroxide / Q000627:therapeutic use; D003782:Dental Pulp / Q000378:metabolism*; D003785:Dental Pulp Capping; D003789:Dental Pulp Exposure / Q000378:metabolism* / Q000628:therapy; D003809:Dentin, Secondary / Q000254:growth & development; D005353:Fibronectins / Q000096:biosynthesis*; D006801:Humans; D007124:Immunoenzyme Techniques; D012387:Root Canal Filling Materials / Q000627:therapeutic use; D019063:Tenascin / Q000096:biosynthesis*; D014945:Wound Healing / Q000502:physiology
D005353:Fibronectins; D012387:Root Canal Filling Materials; D019063:Tenascin; D002126:Calcium Hydroxide