Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH.
Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide.
Zhong
Yingbin
Y
Department of Marine Biology, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China.
Zhang
Xiao-Hua
XH
Chen
Jixiang
J
Chi
Zhenghao
Z
Sun
Boguang
B
Li
Yun
Y
Austin
Brian
B
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005432:Flounder / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D008969:Molecular Sequence Data; D010740:Phospholipases / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D013696:Temperature; D014733:Vibrio / Q000201:enzymology*
D001426:Bacterial Proteins; D006460:Hemolysin Proteins; C073845:hemolysin, Vibrio; D010740:Phospholipases