title: Adipophilin-enriched domains in the ER membrane are sites of lipid droplet biogenesis.
abstract: The prevailing hypothesis of lipid droplet biogenesis proposes that neutral lipids accumulate within the lipid bilayer of the ER membrane from where they are budded off, enclosed by a protein-bearing phospholipid monolayer originating from the cytoplasmic leaflet of the ER membrane. We have used a variety of methods to investigate the nature of the sites of ER-lipid-droplet association in order to gain new insights into the mechanism of lipid droplet formation and growth. The three-dimensional perspectives provided by freeze-fracture electron microscopy demonstrate unequivocally that at sites of close association, the lipid droplet is not situated within the ER membrane; rather, both ER membranes lie external to and follow the contour of the lipid droplet, enclosing it in a manner akin to an egg cup (the ER) holding an egg (the lipid droplet). Freeze-fracture cytochemistry demonstrates that the PAT family protein adipophilin is concentrated in prominent clusters in the cytoplasmic leaflet of the ER membrane closely apposed to the lipid droplet envelope. We identify these structures as sites at which lipids and adipophilin are transferred from ER membranes to lipid droplets. These findings call for a re-evaluation of the prevailing hypothesis of lipid droplet biogenesis.
authors.affiliation: Leibniz Institute for Arteriosclerosis Research, University of Münster, Domagkstr. 3, 48149 Münster, Germany. robenek@uni-muenster.de
authors.forename: Horst
authors.initials: H
authors.lastname: Robenek
authors.forename: Oliver
authors.initials: O
authors.lastname: Hofnagel
authors.forename: Insa
authors.initials: I
authors.lastname: Buers
authors.forename: Mirko J
authors.initials: MJ
authors.lastname: Robenek
authors.forename: David
authors.initials: D
authors.lastname: Troyer
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Severs
mesh_terms: D015153:Blotting, Western; D002478:Cells, Cultured; D020285:Cryoelectron Microscopy; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D005614:Freeze Fracturing / Q000379:methods; D006801:Humans; D008055:Lipids / Q000096:biosynthesis*; D008565:Membrane Proteins; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D010455:Peptides / Q000032:analysis*; D000070780:Perilipin-2
chemical_list: D008055:Lipids; D008565:Membrane Proteins; C000602982:PLIN2 protein, human; D010455:Peptides; D000070780:Perilipin-2