title: [Effect of overdose fluoride on the expression of enamelin in rat mandibular incisor].
abstract: OBJECTIVE
  To observe the effect of overdose fluoride on the expression of enamelin in rat mandibular incisor.
  
  
  METHODS
  Twenty Wistar rats were divided randomly into two groups. Animals were maintained in standard environment with free access to food and distilled water (control group) or water added with 100 mg/L F-(experimental group). The rats were killed in the eighth week. HE staining was used to observe the morphology of ameloblasts. Immunohistochemical staining was adopted to study the expressions of enamelin in rat incisor.
  
  
  RESULTS
  The ameloblasts of the treated rat were arranged in multi-layer. The ameloblasts in group II were thinner than those in group I. The structure of enamel matrix was in disorder. The expressions of enamelin in ameloblasts and odontoblasts were obviously inhibited in group II (P < 0.01).
  
  
  CONCLUSIONS
  The overdose fluoride inhibits the secretion of enamelin and leads to the abnormal development of enamel matrix.
authors.affiliation: Dept. of Oral Medicine, Hospital of Stomatology, Tongji University, Shanghai 200072, China.
authors.forename: Tian-Lin
authors.initials: TL
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Xu
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Guo
authors.forename: Feng-Qiu
authors.initials: FQ
authors.lastname: Jing
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
mesh_terms: D000565:Ameloblasts; D000818:Animals; D003743:Dental Enamel; D003746:Dental Enamel Proteins; D005459:Fluorides; D007180:Incisor; D008334:Mandible; D009804:Odontoblasts; D010710:Phosphates; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003746:Dental Enamel Proteins; D010710:Phosphates; C038456:tuftelin; C012980:fluorophosphate; D005459:Fluorides