Role of free nitrogen and oxygen radicals in the pathogenesis of lipopolysaccharide-induced endotoxemia.
We studied molecular mechanisms of changes in oxidative metabolism under conditions of experimental lipopolysaccharide-induced endotoxemia. Generation of reactive nitrogen and oxygen species in mice increased 18 h after treatment with lipopolysaccharide. These changes contributed to inactivation of enzymes and enzyme complexes (ribonucleotide reductase, NADH-ubiquinone oxidoreductase, and cytochrome c oxidase), dysfunction of the mitochondrial electron transport chain, and development of oxidative stress. Plaferon LB protected mice from the toxic effect of lipopolysaccharide.
Sanikidze
T V
TV
Tbilisi State Medical University. tsanikidze@tsmu.edu
Tkhilava
N G
NG
Papava
M B
MB
Datunashvili
I V
IV
Gongadze
M T
MT
Gamrekelashvili
D D
DD
Bakhutashvili
V I
VI
D000818:Animals; D002374:Catalase / Q000097:blood; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport / Q000187:drug effects; D019446:Endotoxemia / Q000209:etiology* / Q000378:metabolism* / Q000517:prevention & control; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009479:Neuropeptides / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012264:Ribonucleotide Reductases / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood
D008070:Lipopolysaccharides; D009479:Neuropeptides; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; C091912:plaferon; D002374:Catalase; D013482:Superoxide Dismutase; D012264:Ribonucleotide Reductases