title: Assessment of non-reperfused and reperfused myocardial infarction using diffusible or deposited radiolabelled perfusion imaging agents.
abstract: OBJECTIVE
  Incomplete microvascular reperfusion is often observed in patients undergoing thrombolytic therapy or angioplasty for acute myocardial infarction and has important prognostic implications. We compared the myocardial uptake of diffusible ((201)Tl) and deposited ((99m)TcN-NOET) perfusion imaging agents in the setting of experimental infarction.
  
  
  METHODS
  Rats were subjected to permanent coronary occlusion (OCC, n=10) or to 45-min occlusion and reperfusion (REP, n=17). Seven days later, the tracers were co-injected and the animals were euthanised 15 min (all ten rats in the OCC group and 12 rats in the REP group) or 120 min (five rats from the REP group, euthanised at this time point to evaluate any redistribution of the tracers: REP-RED group) afterwards. Infarct size determination and (99m)TcN-NOET/(201)Tl ex vivo imaging were performed. Regional flow and tissue oedema were quantified using radioactive microspheres and (99m)Tc-DTPA, respectively.
  
  
  RESULTS
  (99m)TcN-NOET and (201)Tl defect magnitudes were similar in OCC animals (0.11+/-0.01 vs 0.13+/-0.01). In REP animals, (201)Tl defect magnitude (0.25+/-0.02) was significantly lower than the magnitude of (99m)TcN-NOET and flow defects (0.14+/-0.03 and 0.17+/-0.01, respectively; p<0.05), despite the lack of (201)Tl redistribution (REP-RED animals). (99m)Tc-DTPA indicated the presence of oedema in the reperfused area. Blood distribution studies showed that, unlike (99m)TcN-NOET, (201)Tl plasma activity was mostly unbound to plasma proteins.
  
  
  CONCLUSIONS
  (99m)TcN-NOET and (201)Tl delineated the non-viable area in chronic non-reperfused and reperfused myocardial infarction. The significantly decreased (201)Tl defect in reperfused infarction was likely due to partial diffusion of the tracer from the plasma into the oedema present in the infarcted area. Deposited perfusion tracers might be better suited than diffusible agents for the assessment of regional flow following reperfusion of myocardial infarction.
authors.affiliation: INSERM, U340 Radiopharmaceutiques Biocliniques; Université de Grenoble, La Tronche, France. Laurent.Riou@ujf-grenoble.fr
authors.forename: L M
authors.initials: LM
authors.lastname: Riou
authors.forename: A
authors.initials: A
authors.lastname: Broisat
authors.forename: C
authors.initials: C
authors.lastname: Lartizien
authors.forename: M C
authors.initials: MC
authors.lastname: Toufektsian
authors.forename: S
authors.initials: S
authors.lastname: Maitrejean
authors.forename: M
authors.initials: M
authors.lastname: Janier
authors.forename: G
authors.initials: G
authors.lastname: Vanzetto
authors.forename: D
authors.initials: D
authors.lastname: Fagret
authors.forename: C
authors.initials: C
authors.lastname: Ghezzi
mesh_terms: D000818:Animals; D004058:Diffusion; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D008297:Male; D009203:Myocardial Infarction / Q000150:complications / Q000000981:diagnostic imaging*; D015428:Myocardial Reperfusion Injury / Q000150:complications / Q000000981:diagnostic imaging*; D015609:Organotechnetium Compounds; D010477:Perfusion / Q000379:methods; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D051381:Rats; D017208:Rats, Wistar; D013794:Thallium Radioisotopes; D013859:Thiocarbamates; D018487:Ventricular Dysfunction, Left / Q000000981:diagnostic imaging* / Q000209:etiology
chemical_list: D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D013794:Thallium Radioisotopes; D013859:Thiocarbamates; C093922:technetium Tc 99m, bis(N-ethoxy-N-ethyldithiocarbamato)nitrido-