Palmitylation of cone opsins.
Palmitylation is a widespread modification in G-protein-coupled receptors and often a dynamic process. In rhodopsins, palmitylation is static on C322/C323. Red/green (M/LWS) cone opsins have no cysteines at corresponding positions and no palmitylation. Blue (SWS2) cone opsins have a single corresponding cysteine and mass spectrometric analysis showed partial palmitylation of salamander SWS2 cone opsin. Ultraviolet (SWS1) cone opsins have one corresponding cysteine, but only unpalmitylated opsin was observed for mouse and salamander. The results show that the static palmitylation found on rhodopsin is not found on cone opsins and suggest the possibility of an unidentified role for opsin palmitylation in cones.
Ablonczy
Zsolt
Z
Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA. ablonczy@musc.edu
Kono
Masahiro
M
Knapp
Daniel R
DR
Crouch
Rosalie K
RK
D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002417:Cattle; D002522:Chlorocebus aethiops; D002852:Chromatography, Ion Exchange; D008116:Lizards; D051379:Mice; D008969:Molecular Sequence Data; D019308:Palmitic Acid / Q000378:metabolism*; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism*; D012243:Rhodopsin / Q000235:genetics / Q000378:metabolism; D017299:Rod Opsins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013057:Spectrum Analysis; D014562:Caudata
D011994:Recombinant Proteins; D017299:Rod Opsins; D019308:Palmitic Acid; D012243:Rhodopsin