[Effect of hyaluronic acid chitosan-based microemulsion on the permeability of blood brain barrier in mice].
OBJECTIVE
To study the effect of hyaluronic acid chitosan-based microemulsion (HAC-ME) on the permeability of blood brain barrier( BBB) by using Evans blue (EB) as the indicator.


METHODS
A formamide extraction-ultraviolet spectrophotometry method was employed to determine the concentrations of EB in each of the tissues. The in vivo distribution of HAC-ME groups containing EB in mice and the fluorescence intensity and diffusion domain of brain slices were all studied.


RESULTS
Contrasting to the common microemulsion (ME), HAC-ME at the lower concentration of HAC (<5 mg x mL(-1)) could further improve the transporting of EB across the BBB while EB concentration in other tissues decreased, and Tmax was delayed about 30 min.


CONCLUSIONS
HAC-ME could facilitate the transporting of EB across the BBB and it was concentration dependent. While the brain targeting absorptive capability of HAC-ME was enhanced.
Yao
Jing
J
Department of Pharmaceutics, China Pharmaceutical University, Nanjing 210009, China.
Zhou
Jian-ping
JP
Ping
Qi-neng
QN
Lu
Yun
Y
Yu
Li
L
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D005070:Evans Blue / Q000737:chemistry / Q000493:pharmacokinetics; D005260:Female; D006820:Hyaluronic Acid / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010316:Particle Size; D014018:Tissue Distribution
D004655:Emulsions; D005070:Evans Blue; D006820:Hyaluronic Acid; D048271:Chitosan