Sequence variability of retroviral particles derived from human melanoma cells melanoma-associated retrovirus.
We have shown that melanoma cells produce viral particles that contain sequences which are homologous to human endogenous retroviruses. In this study particles derived from different melanoma cell lines and from melanoma cells of a lymph node metastasis were characterized. We determined the density and the reverse transcriptase (RT) activity of viral particles. Furthermore, we analyzed the sequence variability of multiple clones of each particle preparation. The particles were found to package sequences, which vary for each of the analyzed cell lines. Moreover, even particles derived from the same cell line contain heterologous sequences.
Hirschl
Sonja
S
Department of Dermatology, Division of General Dermatology, University of Vienna Medical School, Währinger Gürtel 18-20, Vienna, Austria. sonja.hirschl@mci.edu
Schanab
Oliver
O
Seppele
Heide
H
Waltenberger
Andrea
A
Humer
Johannes
J
Wolff
Klaus
K
Pehamberger
Hubert
H
Muster
Thomas
T
D000368:Aged; D001483:Base Sequence; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D020077:Endogenous Retroviruses / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D008198:Lymph Nodes / Q000821:virology; D008207:Lymphatic Metastasis; D008545:Melanoma / Q000556:secondary / Q000821:virology*; D008969:Molecular Sequence Data; D016415:Sequence Alignment