Occurrence of A-kinase anchor protein and associated cAMP-dependent protein kinase in the inner compartment of mammalian mitochondria.
Evidence showing the existence in the inner compartment of rat-heart mitochondria of AKAP121 and associated PKA is presented. Immunoblotting analysis and trypsin digestion pattern show that 90% or more of mitochondrial C-PKA, R-PKA and AKAP121 is localized in the inner mitochondrial compartment, when prepared both from isolated mitochondria or cardiomyocyte cultures. This localization is verified by measurement of the specific catalytic activity of PKA, radiolabelling of R-PKA by (32)P-phosphorylated C-PKA and of AKAP by (32)P-phosphorylated R-PKA and electron microscopy of mitochondria exposed to gold-conjugated AKAP121 antibody.
Sardanelli
Anna Maria
AM
Department of Medical Biochemistry, Biology and Physics, University of Bari, Policlinico, Piazza Giulio Cesare 11, 70124 Bari, Italy.
Signorile
Anna
A
Nuzzi
Rosanna
R
Rasmo
Domenico De
DD
Technikova-Dobrova
Zuzana
Z
Drahota
Zdenek
Z
Occhiello
Antonella
A
Pica
Alessandra
A
Papa
Sergio
S
D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D008929:Mitochondria, Heart / Q000378:metabolism* / Q000648:ultrastructure; D051336:Mitochondrial Membranes / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding; D051381:Rats
D054758:A Kinase Anchor Proteins; D048868:Adaptor Proteins, Signal Transducing; C486332:Akap1 protein, rat; D017868:Cyclic AMP-Dependent Protein Kinases