title: Thiamin nutrition and catalysis-induced instability of thiamin diphosphate.
abstract: Thiamin (vitamin B1) is required in animal diets because it is the precursor of the enzyme cofactor, thiamin diphosphate. Unlike other B vitamins, the dietary thiamin requirement is proportional to non-fat energy intake but there is no obvious biochemical reason for this relationship. In the present communication we show for two enzymes that the cofactor undergoes a slow destruction during catalysis, which may explain the interdependence of thiamin and energy intakes.
authors.affiliation: School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: McCourt
authors.forename: Peter F
authors.initials: PF
authors.lastname: Nixon
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Duggleby
mesh_terms: D000095:Acetolactate Synthase / Q000737:chemistry; D000818:Animals; D002384:Catalysis; D003067:Coenzymes / Q000737:chemistry; D004734:Energy Metabolism; D004795:Enzyme Stability; D009751:Nutritional Requirements; D011767:Pyruvate Decarboxylase / Q000737:chemistry; D013831:Thiamine / Q000737:chemistry*; D013835:Thiamine Pyrophosphate / Q000737:chemistry
chemical_list: D003067:Coenzymes; D000095:Acetolactate Synthase; D011767:Pyruvate Decarboxylase; D013835:Thiamine Pyrophosphate; D013831:Thiamine