title: Isolation and expression analysis of multiple isoforms of putative farnesoic acid O-methyltransferase in several crustacean species.
abstract: Farnesoic acid O-methyltransferase (FaMeT) is the enzyme responsible for the conversion of farnesoic acid (FA) to methyl farnesoate (MF) in the final step of MF synthesis. Multiple isoforms of putative FaMeT were isolated from six crustacean species belonging to the families Portunidae, Penaeidae, Scyllaridae and Parastacidae. The portunid crabs Portunus pelagicus and Scylla serrata code for three forms: short, intermediate and long. Two isoforms (short and long) were isolated from the penaeid prawns Penaeus monodon and Fenneropenaeus merguiensis. Two isoforms were also identified in the scyllarid Thenus orientalis and parastacid Cherax quadricarinatus. Putative FaMeT sequences were also amplified from the genomic DNA of P. pelagicus and compared to the putative FaMeT transcripts expressed. Each putative FaMeT cDNA isoform was represented in the genomic DNA, indicative of a multi-gene family. Various tissues from P. pelagicus were individually screened for putative FaMeT expression using PCR and fragment analysis. Each tissue type expressed all three isoforms of putative FaMeT irrespective of sex or moult stage. Protein domain analysis revealed the presence of a deduced casein kinase II phosphorylation site present only in the long isoform of putative FaMeT.
authors.affiliation: Department of Primary Industries and Fisheries (DPI and F), Animal Science, Bribie Island, Qld 4507, Australia.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Kuballa
authors.forename: Kimberley
authors.initials: K
authors.lastname: Guyatt
authors.forename: Bryony
authors.initials: B
authors.lastname: Dixon
authors.forename: Hazra
authors.initials: H
authors.lastname: Thaggard
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Ashton
authors.forename: Brian
authors.initials: B
authors.lastname: Paterson
authors.forename: David J
authors.initials: DJ
authors.lastname: Merritt
authors.forename: Abigail
authors.initials: A
authors.lastname: Elizur
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003445:Crustacea / Q000201:enzymology* / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008780:Methyltransferases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational / Q000502:physiology; D017385:Sequence Homology; D013045:Species Specificity; D013329:Structure-Activity Relationship
chemical_list: D007527:Isoenzymes; D008780:Methyltransferases; C032433:farnesoic acid O-methyltransferase