Expression of the Saccharomyces cerevisiae MPR1 gene encoding N-acetyltransferase in Zygosaccharomyces rouxii confers resistance to L-azetidine-2-carboxylate.
The osmotolerant yeast Zygosaccharomyces rouxii is sensitive to the toxic L-proline analogue, L-azetidine-2-carboxylate (AZC). The possibility of use of the Saccharomyces cerevisiae MPR1 gene (ScMPR1) encoding the AZC-detoxifying enzyme as a dominant selection marker in Z. rouxii was examined. The heterologous expression of ScMPR1 in two Z. rouxii strains resulted in AZC-resistant colonies, but that of ScMPR1 as a dominant marker gene in vectors was affected by a high frequency of spontaneously resistant colonies. The same was found for an AZC-sensitive S. cerevisiae strain in which the ScMPR1 was expressed. In both yeasts, ScMPR1 can be used only as an auxiliary marker gene.
Pribylová
L
L
Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia.
Sychrová
H
H
D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D001383:Azetidinecarboxylic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D004352:Drug Resistance, Microbial / Q000235:genetics; D018014:Gene Transfer Techniques; D005818:Genetic Engineering; D005819:Genetic Markers; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012641:Selection, Genetic; D014169:Transformation, Bacterial; D020068:Zygosaccharomyces / Q000235:genetics* / Q000378:metabolism
D003470:Culture Media; D005819:Genetic Markers; D029701:Saccharomyces cerevisiae Proteins; D001383:Azetidinecarboxylic Acid; D000123:Acetyltransferases; C492447:Mpr1 protein, S cerevisiae