Synaptic pattern of AMPA receptor subtypes upon direction-selective retinal ganglion cells.
In the search for anisotropies that might contribute to a directional preference of direction-selective (DS) retinal ganglion cells (RGCs), we studied the distributions of AMPA receptor subtypes GluR1, GluR2/3, and GluR4 upon the dendritic arbors of DS RGCs of the rabbit with antibody immunocytochemistry. DS RGCs were injected with Lucifer yellow and the cells were identified by their characteristic morphology. The double-labeled images of dendrites and receptors were visualized by confocal microscopy and were reconstructed from high-resolution confocal images. We found no evidence of asymmetry in any of the AMPA receptor subunits examined upon the dendritic arbors of both On and Off layers of DS RGCs. The present results indicate that direction selectivity appears to lie in presynaptic pattern.
Jeong
Seong-Ah
SA
Neuroscience Lab, Department of Biology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-Dong, Daegu, South Korea.
Kwon
Oh-Ju
OJ
Lee
Jea-Young
JY
Kim
Tae-Jin
TJ
Jeon
Chang-Jin
CJ
D000818:Animals; D016764:Cell Polarity / Q000502:physiology*; D003627:Data Interpretation, Statistical; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007546:Isoquinolines; D016547:Kinesins / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009475:Neurons, Afferent / Q000502:physiology; D011817:Rabbits; D018091:Receptors, AMPA / Q000378:metabolism / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000502:physiology*; D013569:Synapses / Q000502:physiology*
D007546:Isoquinolines; D018091:Receptors, AMPA; C074152:kinesin-II; C017475:lucifer yellow; D016547:Kinesins