Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography.
An isocratic, reversed-phase HPLC assay has been developed for the separation of the enantiomers of four lipoxygenase metabolites, without the need for a derivatization step. Separation of the enantiomers was studied on a polysaccharide type chiral stationary phase column. Upon determination of suitable mobile phase composition, the assay was evaluated at various temperatures. In all cases the R enantiomer eluted before the S enantiomer. The best separations were observed at 0 degrees C.
Myrdal
Paul B
PB
University of Arizona, College of Pharmacy, 1703 East Mabel Street, Tucson, AZ 85721, USA. myrdal@pharmacy.arizona.edu <myrdal@pharmacy.arizona.edu>
Angersbach
B Steven
BS
Karlage
Kelly
K
Kuehl
Philip J
PJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008084:Lipoxygenase / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D015203:Reproducibility of Results; D013237:Stereoisomerism
D008084:Lipoxygenase