Ultrastructure of the sperm of blue sprat, Spratelloides gracilis; Teleostei, Clupeiformes, Clupeidae.
The general sperm structure of the investigated clupeoids possess an oliviform head with a distinct deep nuclear fossa, a midpiece with one mitochondrion and a posterior flagellum. They are characterized by two apomorphies: an annular or C-shaped mitochondrion and ITDs (intratubular differentiation) in the A tubules of the axonemal doublets. The fine structure of the spermatozoa of blue sprat, Spratelloides gracilis, was investigated to see if resulting data conformed to the current hypotheses of the taxonomy of the Clupeidae. The mature sperm is characterized by the following features: (1) the nucleus is oliviform; its prominent deep nuclear fossa encloses the initial portion of flagellum, (2) a proximal centriole has not been identified, (3) a single spherical mitochondrion is located laterally in relation to the flagellum and (4) no cytoplasmic canal is present. Our blue sprat-spermatozoan morphology data suggest that the blue sprat has Clupeomorpha affinities, while indicating no close affinity with Elopomorpha. Clupeoid sperm exhibit morphological variations with the nucleus and mitochondrion being particularly variable. This study provides useful systematic characters to the existing knowledge of comparative spermatology and may provide additional clue to Euteleost phylogeny.
Gwo
J-C
JC
Department of Aquaculture, Taiwan National Ocean University, #2, Pei-Nin Road, Keelung 20224, Taiwan. gwonet@ms16.hinet.net
Lin
C-Y
CY
Yang
W-L
WL
Chou
Y-C
YC
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D048430:Cell Shape / Q000502:physiology; D003593:Cytoplasm / Q000502:physiology / Q000648:ultrastructure; D005399:Fishes / Q000033:anatomy & histology* / Q000502:physiology; D005407:Flagella / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000502:physiology / Q000648:ultrastructure; D012098:Reproduction / Q000502:physiology; D013045:Species Specificity; D013094:Spermatozoa / Q000502:physiology / Q000648:ultrastructure*; D013737:Testis / Q000502:physiology / Q000648:ultrastructure*