Differential role of MAP kinase isoforms in malachite green transformed Syrian hamster embryo fibroblasts in culture.
Malachite green (MG) induces DNA damage and malignant transformation of Syrian hamster embryo (SHE) cells in primary culture. In the present study, we have studied the role of all the three isoforms of mitogen activated protein (MAP) kinases i.e. ERK (extracellular regulated kinase), JNK (JUN- N- terminal kinase) and p38 kinase during transformation of SHE cells by MG. The results showed that transformed cells were associated with a decreased expression of phosphoactive ERK and JNK and increased expression of p38 kinase as evident from the Western blot, immunofluorescence and flow cytometry studies. Also, a persistent nuclear localization of p38 kinase was observed in the transformed cells. The present study indicated that p38 kinase was present at higher levels and seemed to be associated with transformation, which suggested that inhibitors of p38 kinase could serve in general as potential agents for selective cancer therapy.
Bose
Bipasha
B
Chemical Carcinogenesis Group, Khanolkar Shodhika, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, India.
Gour
Rekha R
RR
Motiwale
Leena
L
Rao
K V K
KV
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000187:drug effects; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology; D004396:Coloring Agents / Q000633:toxicity*; D006224:Cricetinae; D019938:Cyclin D1 / Q000235:genetics; D003593:Cytoplasm / Q000187:drug effects / Q000201:enzymology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000096:biosynthesis*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000187:drug effects; D007527:Isoenzymes; D048031:JNK Mitogen-Activated Protein Kinases / Q000096:biosynthesis*; D008647:Mesocricetus; D012394:Rosaniline Dyes / Q000633:toxicity*; D048051:p38 Mitogen-Activated Protein Kinases / Q000096:biosynthesis*
D004396:Coloring Agents; D007527:Isoenzymes; D012394:Rosaniline Dyes; C005095:malachite green; D019938:Cyclin D1; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases