Kinetic study of paracetamol on prolidase activity in erythrocytes by capillary electrophoresis with Ru(bpy)(3) (2+) electrochemiluminescence detection.
We explored the CE with (bpy) (2+) (3) electrochemiluminescence detection for the kinetic study of drug-enzyme interaction. Effects of four nonsteroidal anti-inflammatory drugs including aspirin, paracetamol, sodium salicylate and phenacetin on prolidase (PLD) activity in erythrocytes were investigated. Aspirin enhanced PLD activity whereas the other three had inhibiting effects. This may reveal their different effects on the collagen biosynthesis and catabolism that influence tumor invasiveness. Kinetic study of paracetamol on PLD showed that the value of Michaelis constant K(m) for PLD was 1.23 mM. The mechanism of PLD inhibition by paracetamol is noncompetitive inhibition, and the inhibitor constant K(i) value obtained in our research was 9.73 x 10(3) microg/L.
Yuan
Jipei
J
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, China.
Wei
Hui
H
Jin
Wenrui
W
Yang
Xiurong
X
Wang
Erkang
E
D000082:Acetaminophen / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D004150:Dipeptidases / Q000037:antagonists & inhibitors* / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods; D009942:Organometallic Compounds / Q000737:chemistry*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D009942:Organometallic Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II); D000082:Acetaminophen; D004150:Dipeptidases; C022449:proline dipeptidase