Induction of cell apoptosis in non-small cell lung cancer cells by cyclin A1 small interfering RNA.
Cyclin A1 and cyclin B1 are overexpressed in various tumors but are present at low levels in normal tissues. Cyclin A1 is restricted to germ cells undergoing meiosis. In order to explore the possibility of using cyclin A1 and cyclin B1 as anticancer targets, we knocked them down in two lung cancer cell lines, H157 and H596, using siRNA. As with cyclin A1 siRNA in lung cancer cell lines, cyclin B1, Cdc2 and CDK2 were all significantly downregulated. The S phase fraction increased significantly, and they eventually underwent apoptosis by way of downregulated intrinsic apoptotic pathways and modulators with upregulated extrinsic apoptotic pathways. Our study suggests that cyclin A1 might be a promising anticancer target specific to lung cancer.
Cho
Nam Hoon
NH
Department of Pathology, Yonsei University College of Medicine, Seoul, Korea. cho1988@yumc.yonsei.ac.kr
Choi
Yoon Pyo
YP
Moon
Dong Suk
DS
Kim
Haeryoung
H
Kang
Suki
S
Ding
Owen
O
Rha
Sun Young
SY
Yang
Yeon Ju
YJ
Cho
Sang Ho
SH
D017209:Apoptosis / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D019925:Cyclin A / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D056750:Cyclin A1; D020869:Gene Expression Profiling; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
C503745:CCNA1 protein, human; D019925:Cyclin A; D056750:Cyclin A1; D034741:RNA, Small Interfering