MultiCode-RTx real-time PCR system for detection of subpopulations of K65R human immunodeficiency virus type 1 reverse transcriptase mutant viruses in clinical samples.
We report a real-time PCR assay capable of detecting drug-resistant human immunodeficiency virus type 1 reverse transcriptase K65R mutant virus at a level of 0.5% in polymorphic patient plasma specimens. Fifty-three treatment-naïve and 20 treatment-experienced specimens were successfully genotyped with the new method. Results were in agreement with population sequencing and the labor-intensive single-genome sequencing method.
Svarovskaia
Evguenia S
ES
Gilead Sciences, Inc., 4 University Place, 4611 University Drive, Durham, NC 27707, USA. jenny.svarovskaia@gilead.com
Moser
Michael J
MJ
Bae
Andrew S
AS
Prudent
James R
JR
Miller
Michael D
MD
Borroto-Esoda
Katyna
K
D001483:Base Sequence; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood
D012367:RNA, Viral; D054303:HIV Reverse Transcriptase