TOL plasmid transfer during bacterial conjugation in vitro and rhizoremediation of oil compounds in vivo.
Molecular profiling methods for horizontal transfer of aromatics-degrading plasmids were developed and applied during rhizoremediation in vivo and conjugations in vitro. pWW0 was conjugated from Pseudomonas to Rhizobium. The xylE gene was detected both in Rhizobium galegae bv. officinalis and bv. orientalis, but it was neither stably maintained in orientalis nor functional in officinalis. TOL plasmids were a major group of catabolic plasmids among the bacterial strains isolated from the oil-contaminated rhizosphere of Galega orientalis. A new finding was that some Pseudomonas migulae and Pseudomonas oryzihabitans strains harbored a TOL plasmid with both pWW0- and pDK1-type xylE gene. P. oryzihabitans 29 had received the archetypal TOL plasmid pWW0 from Pseudomonas putida PaW85. As an application for environmental biotechnology, the biodegradation potential of oil-polluted soil and the success of bioremediation could be estimated by monitoring changes not only in the type and amount but also in transfer of degradation plasmids.
Jussila
Minna M
MM
Department of Applied Chemistry and Microbiology, Viikki Biocenter, P.O. Box 56 (Viikinkaari 9), FI-00014 University of Helsinki, Helsinki, Finland. minna.m.jussila@helsinki.fi
Zhao
Ji
J
Suominen
Leena
L
Lindström
Kristina
K
D001673:Biodegradation, Environmental; D044822:Biodiversity; D050557:Catechol 2,3-Dioxygenase / Q000737:chemistry; D003227:Conjugation, Genetic / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics; D052918:Environmental Restoration and Remediation / Q000379:methods*; D029968:Escherichia coli Proteins / Q000235:genetics; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial / Q000235:genetics; D006841:Hydrocarbons, Aromatic / Q000737:chemistry; D007219:Industrial Oils; D010105:Oxygenases / Q000737:chemistry; D010957:Plasmids / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D011549:Pseudomonas / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000235:genetics*; D012989:Soil Pollutants / Q000737:chemistry*; D027981:Symporters / Q000235:genetics
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D006841:Hydrocarbons, Aromatic; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D027981:Symporters; C033903:xylE protein, E coli; D010105:Oxygenases; D050557:Catechol 2,3-Dioxygenase; C439587:extradiol dioxygenase; D004262:DNA Restriction Enzymes