[Analysis on the genetic characteristics of the wide-type measles virus circulating in Zhejiang province in 2005].
OBJECTIVE
To study the genetic characteristics of measles viruses circulating in Zhejiang province in 2005.


METHODS
4 groups of measles viruses isolated in outbreaks and the H and N gene were amplified by reverse transcription polymerase chain reaction (RT-PCR). PCR products were purified, sequenced and data was analyzed.


RESULTS
All of the 4 measles isolates belonged to genotype H1 which had been a main genotype containing all of the isolates in China. The isolates shared 99.2% -99.7% identity of amino acid sequence on H and 99.8% identity of amino acid sequence on N gene. When comparing to the China vaccine strain (Shanghai 191), there were 95.2%-95.5% homogeneties and 95.5% homogeneties on H and N gene respectively.


CONCLUSIONS
Data from phylogenic trees of H and N gene revealed that the wide-type measles viruses circulating in Zhejiang province in 2005 all belonged to genotype H1. There were obvious differences on genetic characteristics between the isolates and the genotype A (Shanghai 191).
Feng
Yan
Y
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310009, China.
Yan
Ju-ying
JY
Li
Yi-yu
YY
Shi
Wen
W
Mao
Hai-yan
HY
Zhou
Min
M
Yu
Bei-bei
BB
D000595:Amino Acid Sequence; D002681:China / Q000453:epidemiology; D005814:Genes, Viral; D005838:Genotype; D008457:Measles / Q000453:epidemiology; D008459:Measles virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny