title: [Isolation of bone marrow-derived rat osteoclast-like cells with the digestion of trypsin].
abstract: OBJECTIVE
  To obtain highly enriched osteoclasts in vitro.
  
  
  METHODS
  The bone marrow cells of 1-day, 12-day, 24-day and 36-day old Sprague-Dawley (SD) rats were separately cultured with the presence of 1,25-(OH)2D3 and dexamethasone, and the osteoclast-like cells were identified by Trap staining and scanning electron microscope observation and were purified by trypsin digestion. The count of Trap positive osteoclast-like cells was analyzed statistically.
  
  
  RESULTS
  The osteoclast-like cells of each group were Trap positive cells and could form the bone absorption lacunas in vitro. The amounts of osteoclast-like cells were different statistically between the groups (P < 0.05) except between the groups of 1-day old and 12-day old (P > 0.05). Highly enriched osteoclast-like cells were harvested by the digestion of 0.25% trypsin and 0.02% EDTA.
  
  
  CONCLUSIONS
  It's indicated that a large amount of highly enriched osteoclast-like cells could be obtained through the culture of bone marrow cells of 10-day and 12-day old SD rats with 1,25-(OH)2D3 and dexamethasone and the digestion of trypsin/EDTA.
authors.affiliation: Key Laboratory of Oral Biomedical Engineering, Ministry of Education, Sichuan University, Chengdu 610041, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Dong
authors.forename: Xing
authors.initials: X
authors.lastname: Liang
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.forename: Qing-Hong
authors.initials: QH
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Xia
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Fu
mesh_terms: D000818:Animals; D001853:Bone Marrow; D001854:Bone Marrow Cells; D001842:Bone and Bones; D002117:Calcitriol; D002478:Cells, Cultured; D003907:Dexamethasone; D010010:Osteoclasts; D051381:Rats; D017207:Rats, Sprague-Dawley; D014357:Trypsin
chemical_list: D003907:Dexamethasone; D014357:Trypsin; D002117:Calcitriol