Plasma-sprayed carbon nanotube reinforced hydroxyapatite coatings and their interaction with human osteoblasts in vitro.
Carbon nanotubes (CNT) possess excellent mechanical properties to play the role as reinforcement for imparting strength and toughness to brittle hydroxyapatite (HA) bioceramic coating. However, lack of processing technique to uniformly distribute multiwalled CNTs in HA coating and limited studies and sparse knowledge evincing toxicity of CNTs has kept researchers in dispute for long. In the current work, we have addressed these issues by (i) successfully distributing multiwalled CNT reinforcement in HA coating using plasma spraying to improve the fracture toughness (by 56%) and enhance crystallinity (by 27%), and (ii) culturing human osteoblast hFOB 1.19 cells onto CNT reinforced HA coating to elicit its biocompatibility with living cells. Unrestricted growth of human osteoblast hFOB 1.19 cells has been observed near CNT regions claiming assistance by CNT surfaces to promote cell growth and proliferation.
Balani
Kantesh
K
Department of Mechanical and Materials Engineering, Florida International University, EC 3464, 10555 West Flagler Street, Miami, FL 33174, USA.
Anderson
Rebecca
R
Laha
Tapas
T
Andara
Melanie
M
Tercero
Jorge
J
Crumpler
Eric
E
Agarwal
Arvind
A
D002113:Calcification, Physiologic; D002460:Cell Line; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D013314:Stress, Mechanical; D014961:X-Ray Diffraction
D020099:Coated Materials, Biocompatible; D037742:Nanotubes, Carbon; D017886:Durapatite