A control over accessibility of immobilized enzymes through porous coating layer.
We report immobilization of an enzyme by layer-by-layer (LbL) film deposition technique. All the enzyme layers, including the inner ones, contributed to the activity. We put-forwarded additional coating layers to protect the enzymes. To control the accessibility of the enzymes beneath the coating layer, pores have been introduced. Our results show controlled accessibility of immobilized enzymes in solid-state matrices.
Mohanta
Kallol
K
Department of Solid State Physics, Indian Association for the Cultivation of Science, Kolkata 700032, India.
Pal
Amlan J
AJ
Roy
Sangita
S
Das
Prasanta Kumar
PK
D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D006735:Horseradish Peroxidase