Cbfa-1 (Runx-2) and osteocalcin expression by human osteoblasts in heparin osteoporosis in vitro.
Heparin may cause adverse effects on bone formation following long-term application. The exact pathomechanism is unclear, but in vitro data suggest an impaired osteoblast function. The transcription axis of Cbfa-1 (Runx-2) and osteocalcin is crucial in maintaining an equilibrium of bone formation and resorption in vivo. We used a human osteoblast cell culture model to further investigate the effect of heparin (low-molecular-weight heparin, dalteparin) on the expression of these two regulators of osteoblast differentiation. At high doses, dalteparin caused a significant inhibition of both osteocalcin and Cbfa-1 expression in vitro. Our data support the hypothesis of a direct inhibition of osteoblast function underlying heparin osteoporosis.
Handschin
Alexander E
AE
Department of Surgery, Research Division, University Hospital of Zurich, Switzerland. alexander.handschin@usz.ch
Egermann
Marcus
M
Trentz
Otmar
O
Wanner
Guido A
GA
Kock
Hans-Jürgen
HJ
Zünd
Gregor
G
Trentz
Omana Anna
OA
D002113:Calcification, Physiologic; D002455:Cell Division; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics*; D017931:DNA Primers; D006493:Heparin / Q000009:adverse effects*; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D015675:Osteocalcin / Q000235:genetics*; D010024:Osteoporosis / Q000139:chemically induced* / Q000235:genetics / Q000503:physiopathology; D016133:Polymerase Chain Reaction
D050659:Core Binding Factor Alpha 1 Subunit; D017931:DNA Primers; D015675:Osteocalcin; D006493:Heparin