title: High-performance liquid chromatographic method for the determination of mangiferin in rat plasma and urine.
abstract: A reversed-phase high-performance liquid chromatography assay for mangiferin in rat plasma and urine was developed. Rutin was employed as an internal standard. The mobile phase consisted of acetonitrile-water (16:84, v/v) containing 3% acetic acid at a flow rate of 1 mL/min. Detection was at 257 and 365 nm for mangiferin in plasma and urine, respectively. The limit of quantitation (LOQ) of mangiferin was 0.6 microg/mL in plasma, and 0.48 microg/mL in urine. The standard curve was linear from 0.6 to 24 microg/mL in plasma, and 0.48 to 24 microg/mL in urine, both intra- and inter-day precision of the mangiferin were determined and their RSD did not exceed 10%. The method provides a technique for rapid analysis of mangiferin in rat plasma and urine, which can be used in pharmacokinetic studies.
authors.affiliation: Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Guan
authors.initials: G
authors.lastname: Ye
authors.forename: Yi-Hong
authors.initials: YH
authors.lastname: Tang
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhu
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: Ma
authors.forename: Zhao-Ling
authors.initials: ZL
authors.lastname: Sun
authors.forename: Cheng-Gang
authors.initials: CG
authors.lastname: Huang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D044004:Xanthones / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*
chemical_list: D044004:Xanthones; C013592:mangiferin