title: [Determination of capsaicin in Pheretima aspergillum from different habitats by RP-HPLC].
abstract: OBJECTIVE
  To determine the content of hypoxanthine in Pheretima aspergillum from different habitats.
  
  
  METHODS
  A RP-HPLC method was established. The chromatographic column was Inertsil ODS-EP. The mobile phase was H2O-CH3OH-C4H8O(93:: 7: 0.05). The flow rate was 1.0 ml/min, and the detection wavelength was 254 nm.
  
  
  RESULTS
  The average recoveries for hypoxanthine was 98.6% , precision of the method was 0. 50% (RSD, n = 6).
  
  
  CONCLUSIONS
  The method can be used to determine the content of hypoxanthine in Pheretima aspergillum from diffrent habitats.
authors.affiliation: Department of Pharmaceutics, Guangdong College of Pharmacy, Guangzhou 510006, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Long
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Jie-qian
authors.initials: JQ
authors.lastname: Shu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019271:Hypoxanthine / Q000032:analysis*; D008419:Materia Medica / Q000032:analysis / Q000737:chemistry*; D009835:Oligochaeta / Q000737:chemistry*; D010598:Pharmacognosy; D011786:Quality Control
chemical_list: D008419:Materia Medica; D019271:Hypoxanthine