title: Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells.
abstract: To exclude bacteria- or animal-derived factors from cultured fabrication of transplantable epithelial cell sheets, primary human oral mucosal epithelial cells were seeded on temperature-responsive culture inserts having submicron-scale pores. Supplying culture medium containing human autologous serum to both apical and basal sides of human epithelial cells allows these cells to grow to confluence. These proliferating cells created stratified epithelial layers even when 3T3 feeder layers and fetal bovine serum were eliminated from culture. Normal keratin expression profiles were obtained with these cells, and basal and midlayer cells expressed p63, a putative stem/progenitor marker. These results suggest that temperature-responsive culture inserts can be useful in clinical settings that require the exclusion of xenogeneic factors.
authors.affiliation: Graduate School of Science and Engineering, Waseda University, Tokyo, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Murakami
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Kohji
authors.initials: K
authors.lastname: Nishida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohki
authors.forename: Ryo
authors.initials: R
authors.lastname: Takagi
authors.forename: Joseph
authors.initials: J
authors.lastname: Yang
authors.forename: Hideo
authors.initials: H
authors.lastname: Namiki
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media; D004285:Dogs; D004847:Epithelial Cells / Q000637:transplantation* / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009061:Mouth Mucosa / Q000166:cytology*; D033581:Stem Cell Transplantation / Q000379:methods; D013696:Temperature; D019172:Transplantation Conditioning / Q000379:methods*
chemical_list: D003470:Culture Media