Study on effects of extracts from Salvia Miltiorrhiza and Curcuma Longa in inhibiting phosphorylated extracellular signal regulated kinase expression in rat's hepatic stellate cells.
OBJECTIVE
To study the effect of salvianolic acid B (SAB) and curcumin, the extracts of Salvia Miltiorrhiza and Curcuma Longa, on the proliferation and activation of hepatic stellate cell (HSC), and the extracellular signal regulated kinase (ERK) expression in it.


METHODS
Rat's HSC-T6 were cultured and treated by SAB or curcumin. The inhibitory effect on cell proliferation was determined by 3-(4, 5-dimthyl-2-2thiazoly)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) colorimetry, and the expression levels of alpha smooth actin (alpha-SMA), collagen type I, and ERK were determined by Western blot.


RESULTS
SAB and curcumin inhibited the proliferation and activation of rat's HSC-T6 in dose-dependent fashion and significantly reduced the expression level of alpha-SMA (P < 0.01). Curcumin significantly reduced the expression of collagen type I (P < 0.05). Both SAB and curcumin showed insignificant effect on the ERK expression level, but they could significantly reduce the level of phosphorylated-ERK expression, showing significant difference as compared with that in the control group (P < 0.01 and P < 0.05 respectively).


CONCLUSIONS
SAB and curcumin could significantly inhibit the proliferation, activation of HSC, and the production of type I collagen in HSC, the mechanism may be associated with their inhibition on ERK phosphorylation.
Cheng
Yang
Y
Department of Hepatocirrhosis, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. chengyang@smmail.com
Ping
Jian
J
Liu
Cheng
C
Tan
Ying-zi
YZ
Chen
Gao-feng
GF
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D030024:Curcuma; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology; D008103:Liver Cirrhosis / Q000188:drug therapy / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts; D051381:Rats; D027561:Salvia miltiorrhiza; D014665:Vasodilator Agents / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D014665:Vasodilator Agents; D048049:Extracellular Signal-Regulated MAP Kinases