title: MultiCode-RTx real-time PCR system for detection of subpopulations of K65R human immunodeficiency virus type 1 reverse transcriptase mutant viruses in clinical samples.
abstract: We report a real-time PCR assay capable of detecting drug-resistant human immunodeficiency virus type 1 reverse transcriptase K65R mutant virus at a level of 0.5% in polymorphic patient plasma specimens. Fifty-three treatment-naïve and 20 treatment-experienced specimens were successfully genotyped with the new method. Results were in agreement with population sequencing and the labor-intensive single-genome sequencing method.
authors.affiliation: Gilead Sciences, Inc., 4 University Place, 4611 University Drive, Durham, NC 27707, USA. jenny.svarovskaia@gilead.com
authors.forename: Evguenia S
authors.initials: ES
authors.lastname: Svarovskaia
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Moser
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Bae
authors.forename: James R
authors.initials: JR
authors.lastname: Prudent
authors.forename: Michael D
authors.initials: MD
authors.lastname: Miller
authors.forename: Katyna
authors.initials: K
authors.lastname: Borroto-Esoda
mesh_terms: D001483:Base Sequence; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood
chemical_list: D012367:RNA, Viral; D054303:HIV Reverse Transcriptase