Evaluation of sperm recovery following annexin V magnetic-activated cell sorting separation.
Magnetic-activated cell sorting (MACS) using paramagnetic annexin V-conjugated microbeads eliminates spermatozoa with externalized phosphatidylserine, which is considered one of the features of apoptosis. The objective of this study was to evaluate sperm recovery following the use of MACS as a sperm preparation technique. Mature spermatozoa were separated and divided into two fractions: the first was prepared by density gradient centrifugation (DGC) and MACS, while the second was prepared by DGC only. Following MACS, the percentage of cells collected in the annexin-negative fraction was significantly higher than the annexin-positive fraction and the sperm recovery rate was 73.8 +/- 12.1%. In conclusion, the integration of MACS with DGC can be considered as an effective sperm preparation technique that does not lead to significant cell loss. Separating a distinctive population of non-apoptotic spermatozoa with intact membranes may optimize the outcome of assisted reproduction.
Said
Tamer M
TM
Centre for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics-Gynecology, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA. tamersaid@repromedltd.com
Agarwal
Ashok
A
Grunewald
Sonja
S
Rasch
Manja
M
Glander
Hans-Juergen
HJ
Paasch
Uwe
U
D017304:Annexin A5 / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D008280:Magnetics; D008297:Male; D008863:Microspheres; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism
D017304:Annexin A5