Total synthesis of the macrocyclic cysteine knot microprotein MCoTI-II.
The first total synthesis of MCoTI-II, a cysteine knot microprotein and potent trypsin inhibitor, is described; a synthetic strategy has been developed that combines efficient backbone construction via optimised solid phase peptide synthesis with one-pot 'thia-zip' native chemical ligation and refolding to yield the natural product.
Thongyoo
Panumart
P
Biological and Biophysical Chemistry Section, Department of Chemistry, Imperial College London, UK SW7 2AZ.
Tate
Edward W
EW
Leatherbarrow
Robin J
RJ
D000595:Amino Acid Sequence; D047169:Cyclotides / Q000138:chemical synthesis* / Q000737:chemistry; D008969:Molecular Sequence Data
D047169:Cyclotides; C409923:trypsin inhibitor MCoTI-II