The role of bovine preserved peritoneum in rats ventral hernia: a histological evaluation.
OBJECTIVE
To assess the role of preserved bovine parietal peritoneum as a material for hernia repair in a rat ventral hernia model.


METHODS
An abdominal wall defect (15mm x 25mm) was created in Wistar male rats (n=40). Control animals (n=20) had the polypropylene (PP) mesh sutured into the defect, whereas bovine preserved peritoneum (BPP) was used in experimental group (n=20). After 7 and 28 days, the abdominal wall was taken off and histological studies of the amount of collagen by Sirius Red stain and morphometric evaluation consisted in quantitative analysis of the collagen by using specific software (Imagelab). The Mann-Whitney, Kruskal-Wallis and ANOVA tests were applied for statistical analysis (pd"0.05).


RESULTS
Histological examination revealed no difference between the BPP and PP groups (p = 0.55 NS).


CONCLUSIONS
BPP is suitable for the closure of ventral hernias in rat model as shown by its morphological properties.
Bastos
Eduardo Lemos de Souza
EL
Postgraduate Program in Surgery and Experimentation, Federal University of São Paulo (UNIFESP), Rua Carlos Botelho 246, 17515-240 Marília (SP), Brazil. edbastos@unimedmarilia.com.br
Fagundes
Djalma José
DJ
Taha
Murched Omar
MO
Novo
Neil Ferreira
NF
Juliano
Yara
Y
Simões
Manuel de Jesus
Mde J
Silvado
Rubens Augusto Brazil
RA
D034861:Abdominal Wall / Q000473:pathology* / Q000601:surgery; D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000592:standards*; D001835:Body Weight; D002417:Cattle; D003094:Collagen / Q000032:analysis; D004195:Disease Models, Animal; D006084:Graft Rejection; D006555:Hernia, Ventral / Q000473:pathology / Q000601:surgery*; D008297:Male; D008422:Materials Testing; D010537:Peritoneum / Q000473:pathology* / Q000637:transplantation; D011126:Polypropylenes; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D013526:Surgical Mesh
D001672:Biocompatible Materials; D011126:Polypropylenes; D003094:Collagen