Electrical wiring of Pseudomonas putida and Pseudomonas fluorescens with osmium redox polymers.
Two different flexible osmium redox polymers; poly(1-vinylimidazole)12-[Os-(4,4'-dimethyl-2,2'-di'pyridyl)2Cl2](2+/+) (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N'-methylated-2,2'-biimidazole)3](2+/3+) (osmium redox polymer II) were investigated for their ability to efficiently "wire" Pseudomonas putida ATCC 126633 and Pseudomonas fluorescens (P. putida DSM 6521), which are well-known phenol degrading organisms, when entrapped onto cysteamine modified gold electrodes. The two Os-polymers differ in redox potential and the length of the side chains, where the Os(2+/3+)-functionalities are located. The bacterial cells were adapted to grow in the presence of phenol as the sole source of organic carbon. The performance of the redox polymers as mediators was investigated for making microbial sensors. The analytical characteristics of the microbial sensors were evaluated for determination of catechol, phenol and glucose as substrates in both batch analysis and flow analysis mode.
Timur
Suna
S
Department of Analytical Chemistry, Lund University, P.O. Box 124, SE-221 00, Lund, Sweden.
Haghighi
Behzad
B
Tkac
Jan
J
Pazarlioğlu
Nurdan
N
Telefoncu
Azmi
A
Gorton
Lo
L
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002396:Catechols / Q000032:analysis / Q000378:metabolism; D003543:Cysteamine / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry*; D008828:Microbiological Techniques / Q000295:instrumentation / Q000379:methods*; D009930:Organic Chemicals / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis / Q000378:metabolism; D011145:Polyvinyls / Q000737:chemistry*; D011551:Pseudomonas fluorescens / Q000254:growth & development / Q000302:isolation & purification*; D016958:Pseudomonas putida / Q000254:growth & development / Q000302:isolation & purification*; D013997:Time Factors
D002396:Catechols; D007093:Imidazoles; D009930:Organic Chemicals; D009942:Organometallic Compounds; D010636:Phenols; D011145:Polyvinyls; C072974:polyvinylimidazole; D015082:2,2'-Dipyridyl; D003543:Cysteamine; D002244:Carbon; D006046:Gold; C085573:osmium bis(2,2'-bipyridine)chloride; D005947:Glucose; C034221:catechol