title: Effect of nutritional counselling on hepatic, muscle and adipose tissue fat content and distribution in non-alcoholic fatty liver disease.
abstract: OBJECTIVE
  To assess the effectiveness of the current UK clinical practice in reducing hepatic fat (IHCL).
  
  
  METHODS
  Whole body MRI and (1)H MRS were obtained, before and after 6 mo nutritional counselling, from liver, soleus and tibialis muscles in 10 subjects with non-alcoholic fatty liver disease (NAFLD).
  
  
  RESULTS
  A 500 Kcal-restricted diet resulted in an average weight loss of 4% (-3.4 kg,) accompanied by significant reductions in most adipose tissue (AT) depots, including subcutaneous (-9.9%), abdominal subcutaneous (-10.2%) and intra-abdominal-AT (-11.4%). Intramyocellular lipids (IMCL) were significantly reduced in the tibialis muscle (-28.2%). Decreases in both IHCL (-39.9%) and soleus IMCL (-12.2%) content were also observed, although these were not significant. Several individuals showed dramatic decreases in IHCL, while others paradoxically showed increases in IHCL content. Changes in body composition were accompanied by improvements in certain liver function tests: serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Significant correlations were found between decreases in IHCL and reductions in both intra-abdominal and abdominal subcutaneous AT. Improvements in liver function tests were associated with reductions in intra-abdominal AT, but not with changes in IHCL.
  
  
  CONCLUSIONS
  This study shows that even a very modest reduction in body weight achieved through lifestyle modification can result in changes in body fat depots and improvements in LFTs.
authors.affiliation: Robert Steiner MR Unit, MRC Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, Du Cane Rd, London W12 0HS, United Kingdom. louise.thomas@csc.mrc.ac.uk
authors.forename: E Louise
authors.initials: EL
authors.lastname: Thomas
authors.forename: Audrey E
authors.initials: AE
authors.lastname: Brynes
authors.forename: Gavin
authors.initials: G
authors.lastname: Hamilton
authors.forename: Nayna
authors.initials: N
authors.lastname: Patel
authors.forename: Adam
authors.initials: A
authors.lastname: Spong
authors.forename: Robert D
authors.initials: RD
authors.lastname: Goldin
authors.forename: Gary
authors.initials: G
authors.lastname: Frost
authors.forename: Jimmy D
authors.initials: JD
authors.lastname: Bell
authors.forename: Simon D
authors.initials: SD
authors.lastname: Taylor-Robinson
mesh_terms: D052436:Adipose Tissue, White / Q000737:chemistry / Q000378:metabolism* / Q000503:physiopathology; D001823:Body Composition / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D003376:Counseling / Q000379:methods*; D005234:Fatty Liver / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D008019:Life Style; D050356:Lipid Metabolism / Q000502:physiology*; D008099:Liver / Q000737:chemistry / Q000378:metabolism* / Q000503:physiopathology; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism* / Q000503:physiopathology; D044623:Nutrition Therapy / Q000379:methods*; D017410:Practice Guidelines as Topic; D006113:United Kingdom; D015431:Weight Loss / Q000502:physiology