title: SNPs in human beta-defensin 1 gene (DEFB1): frequencies in a Mexican population and new PCR-RFLPs assays.
abstract: Polymerase chain reaction-restriction fragment length polymorphism assays for two single nucleotide polymorphisms in the human beta-defensin 1 gene have been validated with real-time PCR in 101 healthy individuals from western Mexico. Allele frequencies were 52.5% (692-A) and 98.5% (1836-A). These assays can be confidently used as a cheaper alternative genotyping method for these sites.
authors.affiliation: PhD Program in Human Genetics, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara, Jalisco, México.
authors.forename: E
authors.initials: E
authors.lastname: Prado-Montes de Oca
authors.forename: H
authors.initials: H
authors.lastname: Rangel-Villalobos
authors.forename: M P
authors.initials: MP
authors.lastname: Gallegos-Arreola
authors.forename: L
authors.initials: L
authors.lastname: Sandoval
authors.forename: L E
authors.initials: LE
authors.lastname: Figuera
mesh_terms: D005006:Ethnicity / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008800:Mexico; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D023083:beta-Defensins / Q000235:genetics*
chemical_list: C406407:DEFB1 protein, human; D023083:beta-Defensins