[Tension-force induced cyclooxygenase-2 expression mediated by microfilament in human periodontal ligament fibroblast].
OBJECTIVE
To study the role of microfilament polymerization in menchanotransduction by human periodontal ligament fibroblast (hPDLFs).


METHODS
In tension-force group, hPDLFs were treated by tension-force values of 18% for 8 h, 16 h, 24 h. In tension-force and inhibitor group, the sample was treated with 5 microg/mL cytochalasin B before using tension-forece. Each sample was collected and the expression of cyclooxygenase-2 was measured by using immunohistoche staining.


RESULTS
In tension-force group, the expression of cyclooxygenase-2 enhanced with the extension of loading time. In tension-force and inhibitor group, cyclooxygenase-2 expression was depressed and had no relation with loading time.


CONCLUSIONS
Tension-force induced cyclooxygenase-2 expression is mediated by microfilament, disruption of the microfilament polymerization will destroy mechanotransduction in hPDLFs.
Xiong
Pei-Ying
PY
Dept. of Stomatology, The Second Xiangya Hospital, Central South University, Changsha 410011, China.
Huang
Sheng-Gao
SG
Zhang
Jian-Xing
JX
D008841:Actin Cytoskeleton; D002478:Cells, Cultured; D051546:Cyclooxygenase 2; D005347:Fibroblasts; D006801:Humans; D040542:Mechanotransduction, Cellular; D010513:Periodontal Ligament; D013314:Stress, Mechanical
D051546:Cyclooxygenase 2