Chemical synthesis of picornaviral protein primers of RNA replication.
Naturally occurring nucleopeptidic replication primers (VPg-pUpU) of poliovirus and coxsackie virus were chemically synthesized. The synthesis was accomplished via block-coupling of two minimally protected fragments of the target structures: a short RNA-nucleopeptide and a longer peptide segment containing diverse side-chain functionalities. The synthetic VPg-pUpU of coxsackie virus was characterized by NMR spectroscopy.
Kriek
Nicole M A J
NM
Leiden Institute of Chemistry, Leiden University, PO Box 9502, 2300, RA Leiden, The Netherlands.
Meeuwenoord
Nico J
NJ
van den Elst
Hans
H
Heus
Hans A
HA
van der Marel
Gijsbert A
GA
Filippov
Dmitri V
DV
D001483:Base Sequence; D004770:Enterovirus / Q000737:chemistry* / Q000502:physiology; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D017955:Poliovirus / Q000737:chemistry* / Q000502:physiology; D011522:Protons; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D014764:Viral Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D014779:Virus Replication
D010455:Peptides; D011522:Protons; C034477:RNA primers; D012367:RNA, Viral; D014764:Viral Proteins; D012313:RNA