A posteriori quasi-sectioning of the three-dimensional reconstructions of scanning holographic microscopy.
A method to obtain the axial locations of objects reconstructed by scanning holographic microscopy with submicrometer accuracy is presented and demonstrated. The method combines the holographic advantage of capturing three-dimensional (3D) information in a single two-dimensional scan, with the possibility of optically sectioning the 3D holographic reconstruction a posteriori. The method is demonstrated experimentally for pointlike features (fluorescent beads), and the limitation of the method for features of arbitrary size and shape is discussed.
Indebetouw
Guy
G
Department of Physics, Virginia Tech, Blacksburg, Virginia 24061-0435, USA. gindebet@vt.edu
D000465:Algorithms; D006696:Holography / Q000379:methods*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*