title: Growth-inhibiting activity of active component isolated from Terminalia chebula fruits against intestinal bacteria.
abstract: The growth-inhibitory activity of materials derived from the fruit of Terminalia chebula was evaluated against six intestinal bacteria by means of an impregnated paper disk agar diffusion method. The butanol fraction of T. chebula extract had profound growth-inhibitory activity at a concentration of 5 mg per disk. The biologically active component isolated from the T. chebula fruits was identified with a variety of spectroscopic analyses as ethanedioic acid. The growth responses varied in accordance with the bacterial strain, chemical, and dosage tested. In a test with concentrations of 2 and 1 mg per disk, ethanedioic acid had strong and moderate inhibitory activity against Clostridium perfringens and Escherichia coli, respectively, with no associated adverse effects on the growth of the four tested lactic acid-producing bacteria. Ellagic acid derived from T. chebula fruits exerted a potent inhibitory effect against C. perfringens and E. coli, but little or no inhibition was observed with treatments of behenic acid, P-caryophyllene, eugenol, isoquercitrin, oleic acid, ca-phellandrene, 3-sitosterol, stearic acid, a-terpinene, terpinen-4-ol, terpinolene, or triacontanoic acid. These results may be an indication of at least one of the pharmacological properties of T. chebula fruits.
authors.affiliation: Faculty of Biotechnology and Center for Agricultural Science and Technology, College of Agriculture and Life Science, Chonbuk National University, Chonju 561-756, Korea.
authors.forename: H G
authors.initials: HG
authors.lastname: Kim
authors.forename: J H
authors.initials: JH
authors.lastname: Cho
authors.forename: E Y
authors.initials: EY
authors.lastname: Jeong
authors.forename: J H
authors.initials: JH
authors.lastname: Lim
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
mesh_terms: D003016:Clostridium perfringens / Q000187:drug effects* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D004610:Ellagic Acid / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D005638:Fruit / Q000737:chemistry; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D013058:Mass Spectrometry; D010070:Oxalates / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D029766:Terminalia / Q000737:chemistry*
chemical_list: D006131:Growth Inhibitors; D010070:Oxalates; D010936:Plant Extracts; D004610:Ellagic Acid