title: Preparation and evaluation of C18-bonded 1-microm silica particles for pressurized capillary electrochromatography.
abstract: Nonporous silica spheres (1 microm) were synthesized and bonded with octadecylsilane functionality. These stationary phase particles were packed electrokinetically into fused-silica capillaries with 100 microm id for a length of 20 cm, which was evaluated by using pressurized CEC (pCEC). The efficiency of the C18 RP column was characterized through the theoretical plates of thiourea, benzyl alcohol, toluene, styrene, and naphthalene. The effects of experimental parameters such as the applied voltage, sample size, pump flow rate, pH value and the concentration of the buffer solution, and the content of methanol in the mobile phase, on-column efficiency were evaluated. Column efficiency as high as 200 000 theoretical plates per meter for naphthalene was obtained with the optimal condition of 70% v/v methanol and 30% v/v of 10 mmol/L phosphate buffer (pH 7.8) at an applied voltage of 10 kV and a supplementary pressure of 500 psi.
authors.affiliation: Jiangsu Key Laboratory of Environmental Materials and Environmental Engineering, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, PR China. quqishu@gmail.com
authors.forename: Qishu
authors.initials: Q
authors.lastname: Qu
authors.forename: Xiao
authors.initials: X
authors.lastname: Lu
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Huang
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Hu
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
mesh_terms: D019905:Benzyl Alcohol / Q000302:isolation & purification; D002021:Buffers; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009281:Naphthalenes / Q000302:isolation & purification; D015203:Reproducibility of Results; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000138:chemical synthesis*; D020058:Styrene / Q000302:isolation & purification; D013890:Thiourea / Q000302:isolation & purification; D014050:Toluene / Q000302:isolation & purification
chemical_list: D002021:Buffers; D009281:Naphthalenes; D012821:Silanes; C024779:octadecylsilane; C031721:naphthalene; D014050:Toluene; D020058:Styrene; D012822:Silicon Dioxide; D013890:Thiourea; D019905:Benzyl Alcohol