Purification and characterization of a low molecular weight multifunctional cytotoxic phospholipase A2 from Russell's viper venom.
A basic toxin from Russell's viper venom of 7.2 kDa (RVV-7) has been purified to homogeneity after partial unfolding by 4 M urea followed by filtration through Centricon-30 membrane. Its N-terminal sequence showed strong homology with snake venom cytotoxins. Cytotoxic activity of RVV-7 has been demonstrated with B16F10 melanoma cells. PLA2 activity was observed in cytotoxin (CX3) from Naja kauthia bearing sequence homology with RVV-7. Phospholipase A2 and trypsin inhibitory activities were also observed with RVV-7. Chemical modification and inhibition studies suggested independent functional sites for these activities. A qualitative assessment of tumor growth inhibition by RVV-7 has been made.
Maity
Gargi
G
Division of Structural Biology and Bioinformatics, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Kolkata 700032, India.
Mandal
Somnath
S
Chatterjee
Amitabha
A
Bhattacharyya
Debasish
D
D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008297:Male; D008545:Melanoma; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009368:Neoplasm Transplantation; D010741:Phospholipases A / Q000302:isolation & purification* / Q000378:metabolism; D054467:Phospholipases A2; D017840:Daboia; D016415:Sequence Alignment; D021241:Spectrometry, Mass, Electrospray Ionization; D014361:Trypsin Inhibitors / Q000378:metabolism; D014757:Viper Venoms / Q000201:enzymology*
D000970:Antineoplastic Agents; D003603:Cytotoxins; D014361:Trypsin Inhibitors; D014757:Viper Venoms; D010741:Phospholipases A; D054467:Phospholipases A2