title: Association of the methylenetetrahydrofolate reductase A1298C but not the C677T single nucleotide polymorphism with sickle cell disease in Bahrain.
abstract: The association of methylenetetrahydrofolate reductase (MTHFR) gene mutations, C677T and A1298C, together with changes in homocysteine (Hcy) levels was investigated in 106 sickle cell disease patients and 156 healthy controls from Bahrain. The mutation analysis was done by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR). While the frequencies of the mutant alleles C677T and A1298C were comparable between patients and controls, the frequency of the A1298C (C/C) (p = 0.03) but not C677T (T/T) (p = 0.67) genotype, and of the 677T/1298C haplotype were significantly higher in the patients (p = 0.05). Homocysteine levels were normal in all subjects. This suggests that the A1298C, but not C677T, mutation is associated with the genotype of sickle cell disease.
authors.affiliation: College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Bahrain.
authors.forename: Iman K
authors.initials: IK
authors.lastname: Al-Absi
authors.forename: Abeer M
authors.initials: AM
authors.lastname: Al-Subaie
authors.forename: Ghada
authors.initials: G
authors.lastname: Ameen
authors.forename: Najat
authors.initials: N
authors.lastname: Mahdi
authors.forename: Akbar Mohsin
authors.initials: AM
authors.lastname: Mohammad
authors.forename: Naglaa A
authors.initials: NA
authors.lastname: Fawaz
authors.forename: Wassim Y
authors.initials: WY
authors.lastname: Almawi
mesh_terms: D000293:Adolescent; D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood* / Q000235:genetics*; D001445:Bahrain; D016009:Chi-Square Distribution; D002648:Child; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D016013:Likelihood Functions; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D016017:Odds Ratio; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: D006710:Homocysteine; D042965:Methylenetetrahydrofolate Reductase (NADPH2)