title: Linkage of avian and reproductive tract tropism with sequence divergence adjacent to the 5S ribosomal subunit rrfH of Salmonella enterica.
abstract: The 183 bp between the end of the 23S rrlH rRNA gene and the start of the 5S rrfH rRNA gene (ISR-1) and the 197 bp between the end of the rrfH rRNA gene and the start of the transfer RNA aspU (ISR-2) of Salmonella enterica ssp. enterica serotypes Enteritidis, Typhimurium, Pullorum, Heidelberg, Gallinarum, Typhi and Choleraesuis were compared. ISR-1s of D1 serotypes (Pullorum, Gallinarum and Enteritidis), B serotypes (Typhimurium and Heidelberg) and the C2 serotype Newport and the enteric fever pathogens serotype A Paratyphi and serotype D1 Typhi formed three clades, respectively. ISR-2 further differentiated the avian-adapted serotype Gallinarum from avian-adapted Pullorum and Salmonella bongori from S. enterica. The results suggest that serotypes Heidelberg and Choleraesuis share some evolutionary trends with egg-contaminating serotypes. In addition, ISR-1 and ISR-2 sequences that confirm serotype appear to be linked to clinically relevant host associations of the Salmonellae.
authors.affiliation: Egg Safety and Quality Research Unit, Agricultural Research Service, United States Department of Agriculture, Athens, GA 30605, USA.
authors.forename: Cesar A
authors.initials: CA
authors.lastname: Morales
authors.forename: Richard
authors.initials: R
authors.lastname: Gast
authors.forename: Jean
authors.initials: J
authors.lastname: Guard-Bouldin
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000382:microbiology*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry* / Q000145:classification; D019143:Evolution, Molecular; D005260:Female; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010063:Ovum / Q000382:microbiology; D010802:Phylogeny; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D016415:Sequence Alignment; D012703:Serotyping; D013045:Species Specificity
chemical_list: D021903:DNA, Ribosomal Spacer; D012341:RNA, Ribosomal, 5S