Structure of the O-polysaccharide of Escherichia coli O150 containing 2-acetamido-4-O-[(S)-1-carboxyethyl]-2-deoxy-d-glucose.
An acidic O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Escherichia coli O150 and studied by sugar and methylation analyses, triflic acid solvolysis, Smith degradation, (1)H and (13)C NMR spectroscopy, including 2D ROESY, (1)H,(13)C HSQC, HMQC-TOCSY, and HMBC experiments. The polysaccharide was found to contain a regioisomer of N-acetylisomuramic acid, 2-acetamido-4-O-[(S)-1-carboxyethyl]-2-deoxy-d-glucose [d-GlcNAc4(Slac)]. The structure of its hexasaccharide repeating unit was established.
Perepelov
Andrei V
AV
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru
Han
Weiqing
W
Senchenkova
Sof'ya N
SN
Shevelev
Sergei D
SD
Shashkov
Alexander S
AS
Feng
Lu
L
Liu
Yanqun
Y
Knirel
Yuriy A
YA
Wang
Lei
L
D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry*; D008969:Molecular Sequence Data; D009112:Muramic Acids / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D009112:Muramic Acids; D019081:O Antigens; C031651:N-acetylmuramic acid