Involvement of insect-derived growth factor (IDGF) in the cell growth of an embryonic cell line of flesh fly.
Insect-derived growth factor (IDGF) is the first adenosine deaminase-related growth factor (ADGF) purified from the conditioned medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina (flesh fly). Here we show the requirement of IDGF for the growth of NIH-Sape-4 cells. Growth factor activity was abolished by adsorption of IDGF from the conditioned medium of NIH-Sape-4 cells. In addition, knockdown of IDGF gene expression by RNA interference (RNAi) significantly reduced IDGF secretion from the cells following cell growth inhibition. The IDGF gene was strongly expressed in the hemocytes, and IDGF increased the viability of the larval hemocytes. These data provide evidence that IDGF is required for the growth of NIH-Sape-4 cells and possibly for hemocyte viability.
Tanaka
Yasuhiro
Y
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Yamaguchi
Shinji
S
Fujii-Taira
Ikuko
I
Iijima
Ryoko
R
Natori
Shunji
S
Homma
Koichi J
KJ
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004175:Diptera / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000166:cytology; D015870:Gene Expression; D006133:Growth Substances / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D006434:Hemocytes / Q000166:cytology / Q000187:drug effects; D019476:Insect Proteins / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D007814:Larva / Q000166:cytology / Q000187:drug effects; D034622:RNA Interference
D006133:Growth Substances; D019476:Insect Proteins