title: [Effect of nitric oxide and nitric oxide synthase on sensorineural hearing loss secondary to secretory otitis media].
abstract: OBJECTIVE
  To evaluate the effect of nitric oxide synthase (NOS) and nitric oxide(NO) on sensorineural hearing loss (SNHL) secondary to secretory otitis media (SOM).
  
  
  METHODS
  Samples of middle ear effusion were collected at the time of tympanocentesis from 16 cases of SOM with SNHL(group A) and 16 cases of SOM without SNHL(group B). And the concentration of NO and NOS activity in middle ear effusion were detected by colorimetric assay (Griess method).
  
  
  RESULTS
  The concentration of NO in group A (181.19 +/- 44.31) micromol/L was significantly higher than that in group B (137.00 +/- 40.67) micromol/L (P < 0.01). The NOS activity in group A (97.69 +/- 29.62)U was also significantly higher than that in group B (75.50 +/- 26.99)U (P < 0.05).
  
  
  CONCLUSIONS
  Excess generation of NO in middle ear effusion which synthesized by NOS may hurt inner ear directly by its cytotoxic action or enhance local inflammatory reaction with other inflammatory factors. This study suggests that NO play a significant role in the process and pathogenesis of SNHL secondary to SOM.
authors.affiliation: Department of Otolaryngology, Drum Tower Hospital, Affiliated to Nanjing University Medical College, Nanjing, 210008, China. kaisachen@163.com
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Wandong
authors.initials: W
authors.lastname: She
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Dai
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Shen
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Qian
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.forename: Xia
authors.initials: X
authors.lastname: Gao
mesh_terms: D000328:Adult; D001301:Audiometry, Pure-Tone; D005260:Female; D006319:Hearing Loss, Sensorineural / Q000209:etiology / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D010034:Otitis Media with Effusion / Q000150:complications / Q000378:metabolism*
chemical_list: D009569:Nitric Oxide; D019001:Nitric Oxide Synthase