title: An interdisciplinary approach to investigate the impact of cobalt in a human keratinocyte cell line.
abstract: Since in nuclear power plants, risks of skin contact contamination by radiocobalt are significant, we focused on the impact of cobalt on a human cutaneous cell line, i.e. HaCaT keratinocytes. The present paper reports an interdisciplinary approach aimed at clarifying the biochemical mechanisms of metabolism and toxicity of cobalt in HaCaT cells. Firstly, a brief overview of the used instrumental techniques is reported. The following parts present description and discussion of results concerning: (i) toxicological studies concerning cobalt impact towards HaCaT cells (ii) structural and speciation fundamental studies of cobalt-bioligand systems, through X-ray absorption spectroscopy (XAS), ab initio and thermodynamic modelling (iii) preliminary results regarding intracellular cobalt speciation in HaCaT cells using size exclusion chromatography/inductively coupled plasma-atomic emission spectroscopy (SEC/ICP-AES) and direct in situ analysis by ion beam micropobe analytical techniques.
authors.affiliation: DEN/DANS/DPC/SECR/LSRM, CEA Saclay, Batiment 391, 91191 Gif-sur-Yvette, France.
authors.forename: C
authors.initials: C
authors.lastname: Bresson
authors.forename: C
authors.initials: C
authors.lastname: Lamouroux
authors.forename: C
authors.initials: C
authors.lastname: Sandre
authors.forename: M
authors.initials: M
authors.lastname: Tabarant
authors.forename: N
authors.initials: N
authors.lastname: Gault
authors.forename: J L
authors.initials: JL
authors.lastname: Poncy
authors.forename: J L
authors.initials: JL
authors.lastname: Lefaix
authors.forename: C
authors.initials: C
authors.lastname: Den Auwer
authors.forename: R
authors.initials: R
authors.lastname: Spezia
authors.forename: M-P
authors.initials: MP
authors.lastname: Gaigeot
authors.forename: E
authors.initials: E
authors.lastname: Ansoborlo
authors.forename: S
authors.initials: S
authors.lastname: Mounicou
authors.forename: A
authors.initials: A
authors.lastname: Fraysse
authors.forename: G
authors.initials: G
authors.lastname: Deves
authors.forename: T
authors.initials: T
authors.lastname: Bacquart
authors.forename: H
authors.initials: H
authors.lastname: Seznec
authors.forename: T
authors.initials: T
authors.lastname: Pouthier
authors.forename: P
authors.initials: P
authors.lastname: Moretto
authors.forename: R
authors.initials: R
authors.lastname: Ortega
authors.forename: R
authors.initials: R
authors.lastname: Lobinski
authors.forename: C
authors.initials: C
authors.lastname: Moulin
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003035:Cobalt / Q000493:pharmacokinetics / Q000633:toxicity*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D009153:Mutagens; D003035:Cobalt