Mathematical modeling of living cell metabolism using the method of steady-state stoichiometric flux balance.
This approach uses a set of algebraic linear equations for reaction rates (the method of steady-state stoichiometric flux balance) to model the purposeful metabolism of the living self-reproducing biochemical system (i.e. cell), which persists in steady-state growth. Linear programming (SIMPLEX method) is used to derive the solution for the model equations set (determining reaction rates which provide flux balance at given conditions). Here, we demonstrate the approach through the mathematical modeling of steady-state metabolism in Saccharomyces cerevisiae mitochondria.
Drozdov-Tikhomirov
L N
LN
Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq 2, Moscow 123182, Russia. drozdov@img.ras.ru
Scurida
G I
GI
Davidov
A V
AV
Alexandrov
A A
AA
Zvyagilskaya
R A
RA
D000465:Algorithms; D003198:Computer Simulation; D004734:Energy Metabolism / Q000502:physiology*; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D008954:Models, Biological; D009097:Multienzyme Complexes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
D024101:Mitochondrial Proteins; D009097:Multienzyme Complexes; D029701:Saccharomyces cerevisiae Proteins