title: Evaluation of ex vivo human skin permeation of genistein and daidzein.
abstract: The percutaneous absorption of genistein (GEN) and daidzein (DAI), whose oestrogenic-like activity is well known, is scantily investigated. In this work the ability of GEN and DAI to reach therapeutic steady-state plasma concentrations following transdermal administration was studied. The skin permeation studies were conducted by using modified Franz diffusion cell and human epidermis as a membrane. PEG400 was the most effective vehicle for both molecules. On the basis of the ex vivo permeation results and estimating therapeutic plasma concentration, we assume that pure GEN can be efficaciously administered by the transdermal route.
authors.affiliation: Istituto di Chimica Farmaceutica e Tossicologica, Università degli Studi di Milano, Milano, Italy. paola.minghetti@unimi.it
authors.forename: Paola
authors.initials: P
authors.lastname: Minghetti
authors.forename: Francesco
authors.initials: F
authors.lastname: Cilurzo
authors.forename: Antonella
authors.initials: A
authors.lastname: Casiraghi
authors.forename: Luisa
authors.initials: L
authors.lastname: Montanari
mesh_terms: D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D015194:Diffusion Chambers, Culture; D004817:Epidermis / Q000378:metabolism; D004968:Estrogens, Non-Steroidal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D019833:Genistein / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D066298:In Vitro Techniques; D007529:Isoflavones / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D014677:Pharmaceutical Vehicles; D010936:Plant Extracts / Q000737:chemistry; D011092:Polyethylene Glycols; D012869:Skin Absorption / Q000502:physiology*; D012995:Solubility; D013025:Glycine max / Q000737:chemistry
chemical_list: D004968:Estrogens, Non-Steroidal; D007529:Isoflavones; D014677:Pharmaceutical Vehicles; D010936:Plant Extracts; D011092:Polyethylene Glycols; C004742:daidzein; D019833:Genistein