title: Activation of the lectin pathway by natural IgM in a model of ischemia/reperfusion injury.
abstract: Reperfusion of ischemic tissues elicits an acute inflammatory response involving serum complement, which is activated by circulating natural IgM specific to self-Ags exposed by ischemia. Recent reports demonstrating a role for the lectin pathway raise a question regarding the initial events in complement activation. To dissect the individual roles of natural IgM and lectin in activation of complement, mice bearing genetic deficiency in early complement, IgM, or mannan-binding lectin were characterized in a mesenteric model of ischemia reperfusion injury. The results reveal that IgM binds initially to ischemic Ag providing a binding site for mannan-binding lectin which subsequently leads to activation of complement and injury.
authors.affiliation: CBR Institute of Biomedical Research Inc., Harvard Medical School, 800 Huntington Avenue, Boston, MA 02115, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Kazue
authors.initials: K
authors.lastname: Takahashi
authors.forename: Elisabeth M
authors.initials: EM
authors.lastname: Alicot
authors.forename: Thomas
authors.initials: T
authors.lastname: Vorup-Jensen
authors.forename: Benedikt
authors.initials: B
authors.lastname: Kessler
authors.forename: Steffen
authors.initials: S
authors.lastname: Thiel
authors.forename: Jens Christian
authors.initials: JC
authors.lastname: Jensenius
authors.forename: R Alan B
authors.initials: RA
authors.lastname: Ezekowitz
authors.forename: Francis D
authors.initials: FD
authors.lastname: Moore
authors.forename: Michael C
authors.initials: MC
authors.lastname: Carroll
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000936:Antigen-Antibody Complex; D001324:Autoantigens / Q000235:genetics / Q000276:immunology; D037582:Complement Pathway, Mannose-Binding Lectin / Q000276:immunology*; D004195:Disease Models, Animal; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D037601:Mannose-Binding Lectin / Q000276:immunology / Q000378:metabolism*; D013058:Mass Spectrometry; D008643:Mesentery / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D018995:Myosin Heavy Chains / Q000235:genetics / Q000276:immunology; D024462:Myosin Type II / Q000235:genetics / Q000276:immunology; D015427:Reperfusion Injury / Q000276:immunology* / Q000378:metabolism / Q000473:pathology
chemical_list: D000936:Antigen-Antibody Complex; D001324:Autoantigens; D007075:Immunoglobulin M; D037601:Mannose-Binding Lectin; C483905:Myh14 protein, mouse; D024462:Myosin Type II; D018995:Myosin Heavy Chains