title: High incidence of genotypic variance between sequential herpes simplex virus type 2 isolates from HIV-1-seropositive patients with recurrent genital herpes.
abstract: We developed a polymerase chain reaction (PCR) method, based on strain-to-strain variation of DNA repeats in the herpes simplex virus type 2 (HSV-2) genes US1 and US12, to genotype HSV-2 strains and determine the incidence and risk factors associated with HSV-2 superinfection in patients with recurrent genital herpes (RGH). Forty-seven (92%) of 51 unrelated HSV-2 isolates could be distinguished. Genotyping of sequential HSV-2 isolates showed a different genotype in all of the 11 human immunodeficiency virus type 1 (HIV-1)-seropositive patients with RGH, compared with 1 of the 8 HIV-1-seronegative patients with RGH. The PCR approach developed distinguishes unrelated HSV-2 strains efficiently and indicated a high incidence of genotype variance between sequential HSV-2 isolates of HIV-1-seropositive patients with RGH.
authors.affiliation: Department of Dermatology and Venereology, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: R Wim
authors.initials: RW
authors.lastname: Roest
authors.forename: Jeroen
authors.initials: J
authors.lastname: Maertzdorf
authors.forename: Marius
authors.initials: M
authors.lastname: Kant
authors.forename: Willem I
authors.initials: WI
authors.lastname: van der Meijden
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: Georges M G M
authors.initials: GM
authors.lastname: Verjans
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000150:complications*; D015497:HIV-1; D006558:Herpes Genitalis / Q000150:complications / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D018258:Herpesvirus 2, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D015994:Incidence; D008297:Male; D009426:Netherlands / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012008:Recurrence
chemical_list: D004279:DNA, Viral