Soybean (Glycine max) transformation using mature cotyledonary node explants.
Agrobacterium tumefaciens-mediated transformation of soybeans has been steadily improved since its development in 1988. Soybean transformation is now possible in a range of genotypes from different maturity groups using different explants as sources of regenerable cells, various selectable marker genes and selective agents, and different A. tumefaciens strains. The cotyledonary-node method has been extensively investigated and across a number of laboratories yields on average greater than 1% transformation efficiency (one Southern-positive, independent event per 100 cotyledonary-node explants). Continued improvements in the cotyledonary-node method concomitant with further increases in transformation efficiency will enhance broader adoption of this already productive transformation method for use in crop improvement and functional genomics research efforts.
Olhoft
Paula M
PM
BASF Plant Service, Research Triangle Park, NC, USA.
Donovan
Christopher M
CM
Somers
David A
DA
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000254:growth & development; D018548:Cotyledon / Q000235:genetics* / Q000382:microbiology; D004351:Drug Resistance / Q000235:genetics; D018014:Gene Transfer Techniques; D005819:Genetic Markers; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology; D013025:Glycine max / Q000235:genetics* / Q000382:microbiology; D014170:Transformation, Genetic
D005819:Genetic Markers