Armored RNA as positive control and standard for quantitative reverse transcription-polymerase chain reaction assay for rubella virus.
RV Armored RNA constructed in the laboratory was shown to be resistant to RNase and DNase digestion. It could remain stable at 4 degrees C for at least 60 days, and was much more stable than RV strain TR23 [corrected] in normal human plasma. Therefore it could be used as stable, well-characterized positive control and standard for quantitative RT-PCR assay.
Zhao
L
L
Department of Laboratory, Tai'an Central Hospital, Tai'an, China [corrected]
Ma
Y
Y
Zhao
S
S
Yang
N
N
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D020871:RNA Stability; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification / Q000592:standards; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012412:Rubella virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics
D017931:DNA Primers; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C061826:E1 envelope protein, Rubella virus