[Effect of Ligustrazine on type I, III collagen synthesis in lung tissue of silica-treated rat].
OBJECTIVE
To study the effect of Ligustrazine on type I, III collagen synthesis in lung tissue of silica-treated rat.


METHODS
The 128 experimental rats were randomly divided into control, silica and Ligustrazine group. 1ml silica (50g/L) was injected intratracheally in silica group and Ligustrazine group, while Ligustrazine group were injected intraperitoneally 50mg/(kg x d) Ligustrazine. Samples were collected on the 1st, 3rd, 7th, 14th, 21st, 28th day after injected silica. Type I , III collagen on paraffin-embedded lung sections were stained with sirius red, detected by polarized light microscopy and quantified by Image-Pro Plus.


RESULTS
On the 3rd day after silica instillation, type III collagen began to appear, while type I collagen formed on the 7th day. Thereafter Type I, III collagen increased progressively. Compared with control group, type I, III collagen expression of silica group increased. At different time points type I, III collagen expression of Ligustrazine group decreased than silica group. There was significant difference of type I collagen area percentage on the 7th, 14th, 28th day, type III collagen area percentage on the 28th day between Ligustrazine group and silica group.


CONCLUSIONS
Silica dioxide could induce the increase of type I, III collagen production in lung tissue, and their stages are different. Ligustrazine could suppress type I, III collagen production in lung tissue of silica-treated rat, and it's mechanism would be studied.
Gao
Xiu-xia
XX
Affiliated Hospital of Medical College of Chinese People's Armed Polices Forces, Tianjin 300162,China.
Du
Hai-ke
HK
Wang
Yi-fei
YF
Li
Hong-wei
HW
D000818:Animals; D024042:Collagen Type I / Q000096:biosynthesis*; D024061:Collagen Type III / Q000096:biosynthesis*; D005260:Female; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000517:prevention & control; D011719:Pyrazines / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000633:toxicity*
D024042:Collagen Type I; D024061:Collagen Type III; D011719:Pyrazines; D012822:Silicon Dioxide; C017953:tetramethylpyrazine