A novel fluorescent sensitive assay for detection of differential T cell mediated lysis of multiple adherent target cells.
There are few studies that have investigated T cell mediated lysis of adherent cells. We have developed a novel, rapid and sensitive fluorescent dye-swap assay that allows efficient detection of adherent target cell lysis. The assay allows simultaneous use of multiple differentially sensitised targets and facilitates concomitant surface or intracellular effector cell phenotypic analysis.
Black
Antony P
AP
Cutaneous Immunology Group, MRC Human Immunology Unit, University of Oxford, Weatherall Institute of Molecular Medicine, Oxford OX3 9DS, UK. ablack@hammer.imm.ox.ac.uk
Jones
Louise
L
Ardern-Jones
Michael
M
Ogg
Graham S
GS
D002448:Cell Adhesion / Q000276:immunology; D002460:Cell Line; D005434:Flow Cytometry / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D015603:Keratinocytes / Q000276:immunology*; D012235:Rhodamines / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
C116572:(((4-chloromethyl)benzoyl)amino)-tetramethylrhodamine; C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D012235:Rhodamines; D013388:Succinimides