Selective 1H-NMR relaxation investigations of membrane-bound drugs in vitro. 1. Colchicine.
Binding of colchicine to dipalmitoylphosphatidylcholine bilayer vesicles was detected by measuring the 1H-NMR selective spin-lattice relaxation rates of the low-field protons of colchicine. From the temperature dependence of the selective rates, preferential binding was observed above the temperature of transition. In the same way, binding of colchicine to red blood cells was detected and the equilibrium constant determined. Binding to the lipid matrix of red blood cells accounted only partially for the binding of colchicine to whole cells.
Valensin
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Department of Chemistry, University of Siena Pian dei Mantellini 44, 53100 Siena, Italy.
Lepri
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Gaggelli
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