Synthesis of RNA using 2'-O-DTM protection.
tert-Butyldithiomethyl (DTM), a novel hydroxyl protecting group, cleavable under reductive conditions, was developed and applied for the protection of 2'-OH during solid-phase RNA synthesis. This function is compatible with all standard protecting groups used in oligonucleotide synthesis, and allows for fast and high-yield synthesis of RNA. Oligonucleotides containing the 2'-O-DTM groups can be easily deprotected under the mildest possible aqueous and homogeneous conditions. The preserved 5'-O-DMTr function can be used for high-throughput cartridge RNA purification.
Semenyuk
Andrey
A
Department of Genetics and Pathology, Uppsala University, Sweden.
Földesi
Andras
A
Johansson
Tommy
T
Estmer-Nilsson
Camilla
C
Blomgren
Peter
P
Brännvall
Mathias
M
Kirsebom
Leif A
LA
Kwiatkowski
Marek
M
D000473:Alkanes / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000138:chemical synthesis* / Q000302:isolation & purification; D013457:Sulfur Compounds / Q000138:chemical synthesis / Q000737:chemistry*
D000473:Alkanes; D013457:Sulfur Compounds; D012313:RNA