Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells.
Erythroblast enucleation is thought to be largely dependent on signals mediated by other cells, such as macrophages. In an attempt to improve the in vitro production of red blood cells (RBCs) from immature hematopoietic progenitor cells, we have developed a method to produce enucleated RBCs efficiently in the absence of feeder cells. Our method may represent an efficient way to produce transfusable RBCs on a large scale from hematopoietic progenitors.
Miharada
Kenichi
K
Cell Engineering Division, RIKEN BioResource Center, Koyadai 3-1-1, Tsukuba, Ibaraki 305-0074, Japan.
Hiroyama
Takashi
T
Sudo
Kazuhiro
K
Nagasawa
Toshiro
T
Nakamura
Yukio
Y
D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002467:Cell Nucleus; D004900:Erythroblasts / Q000166:cytology* / Q000502:physiology; D006021:Glycophorins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D011990:Receptors, Transferrin / Q000378:metabolism
D015703:Antigens, CD; D018952:Antigens, CD34; C068126:CD71 antigen; D006021:Glycophorins; D011990:Receptors, Transferrin