[Preparation and evaluation of microbubble ultrasound contrast agent with N-carboxymethyl chitosan].
OBJECTIVE
To prepare microbubble, made of N-carboxymethyl chitosan, as ultrasound contrast agent and evaluate its characteristics and acoustic effects in vivo.


METHODS
Oil-Water-Oil multiple emulsion/solvent evaporation method was used to prepare the microbubble contrast agent. Both optical micrography and scanning electron micrography were performed to determine the bubble size and morphology. The acoustic effect of the N-carboxymethyl chitosan echo contrast agent was evaluated in vivo in rabbit. Liver echo images were recorded with ultrasound machine before and after intravenous bolus injecting 0.5 ml of the agent.


RESULTS
The novel N-carboxymethyl chitosan echo contrast agent was formulated as lyophilized product, with a mean diameter of 2-3 microm and a shell thickness of 250-300 nm. Its size is relatively uniform. The imaging effect was remarkably enhanced with the ultrasonic contrast agent when applied in rabbit livers.


CONCLUSIONS
It is feasible to prepare excellent microbubble ultrasound contrast agent with N-carboxymethyl chitosan as membrane components.
Lü
Feng
F
Tianjin Biomedical Materials Key Laboratory, Institute of Biomedical Engineering, CAMS and PUMC, Tianjin 300192, China.
Chen
Su-Xia
SX
Liu
Tian-Jun
TJ
Sun
Hong-Fan
HF
D000818:Animals; D002686:Chitin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D003287:Contrast Media; D008099:Liver / Q000000981:diagnostic imaging; D045423:Microbubbles; D011817:Rabbits; D014465:Ultrasonics; D014463:Ultrasonography
D003287:Contrast Media; C067123:N-carboxymethylchitosan-N-O-sulfate; D002686:Chitin