Comparative structural analysis of a novel glutathioneS-transferase (ATU5508) from Agrobacterium tumefaciens at 2.0 A resolution.
Glutathione S-transferases (GSTs) comprise a diverse superfamily of enzymes found in organisms from all kingdoms of life. GSTs are involved in diverse processes, notably small-molecule biosynthesis or detoxification, and are frequently also used in protein engineering studies or as biotechnology tools. Here, we report the high-resolution X-ray structure of Atu5508 from the pathogenic soil bacterium Agrobacterium tumefaciens (atGST1). Through use of comparative sequence and structural analysis of the GST superfamily, we identified local sequence and structural signatures, which allowed us to distinguish between different GST classes. This approach enables GST classification based on structure, without requiring additional biochemical or immunological data. Consequently, analysis of the atGST1 crystal structure suggests a new GST class, distinct from previously characterized GSTs, which would make it an attractive target for further biochemical studies.
Kosloff
Mickey
M
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York.
Han
Gye Won
GW
Krishna
S Sri
SS
Schwarzenbacher
Robert
R
Fasnacht
Marc
M
Elsliger
Marc-André
MA
Abdubek
Polat
P
Agarwalla
Sanjay
S
Ambing
Eileen
E
Astakhova
Tamara
T
Axelrod
Herbert L
HL
Canaves
Jaume M
JM
Carlton
Dennis
D
Chiu
Hsiu-Ju
HJ
Clayton
Thomas
T
DiDonato
Michael
M
Duan
Lian
L
Feuerhelm
Julie
J
Grittini
Carina
C
Grzechnik
Slawomir K
SK
Hale
Joanna
J
Hampton
Eric
E
Haugen
Justin
J
Jaroszewski
Lukasz
L
Jin
Kevin K
KK
Johnson
Hope
H
Klock
Heath E
HE
Knuth
Mark W
MW
Koesema
Eric
E
Kreusch
Andreas
A
Kuhn
Peter
P
Levin
Inna
I
McMullan
Daniel
D
Miller
Mitchell D
MD
Morse
Andrew T
AT
Moy
Kin
K
Nigoghossian
Edward
E
Okach
Linda
L
Oommachen
Silvya
S
Page
Rebecca
R
Paulsen
Jessica
J
Quijano
Kevin
K
Reyes
Ron
R
Rife
Christopher L
CL
Sims
Eric
E
Spraggon
Glen
G
Sridhar
Vandana
V
Stevens
Raymond C
RC
van den Bedem
Henry
H
Velasquez
Jeff
J
White
Aprilfawn
A
Wolf
Guenter
G
Xu
Qingping
Q
Hodgson
Keith O
KO
Wooley
John
J
Deacon
Ashley M
AM
Godzik
Adam
A
Lesley
Scott A
SA
Wilson
Ian A
IA
D016960:Agrobacterium tumefaciens / Q000737:chemistry / Q000166:cytology / Q000201:enzymology*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000145:classification; D018360:Crystallography, X-Ray; D019281:Dimerization; D005982:Glutathione Transferase / Q000737:chemistry* / Q000145:classification; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D017433:Protein Structure, Secondary
D001426:Bacterial Proteins; D005982:Glutathione Transferase