title: The use of bone marrow-chimeric mice in elucidating immune mechanisms.
abstract: DNA vaccines hold promise for generating protective immunity against a wide variety of pathogens. Understanding the mechanism by which vaccine-encoded antigens are processed and presented to naïve lymphocytes in the host is critical for rational design of efficacious vaccines. This chapter provides practical guide in making irradiation-induced bone marrow chimeric mice, which can be used to dissect a variety of aspects of antigen presentation by the hematopoietic vs nonhematopoietic compartments.
authors.affiliation: Section of Immunobiology, Yale University, New Haven, CT, USA.
authors.forename: Akiko
authors.initials: A
authors.lastname: Iwasaki
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology* / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D006410:Hematopoiesis / Q000276:immunology* / Q000528:radiation effects; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D018183:Transplantation Chimera / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*; D014916:Whole-Body Irradiation
chemical_list: D019444:Vaccines, DNA