title: Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances.
abstract: To simplify the molecular detection of micro-organisms, we evaluated the tolerance of loop-mediated isothermal amplification (LAMP) to a culture medium and some biological substances. The sensitivity of LAMP was less affected by the various components of the clinical samples than was polymerase chain reaction (PCR); therefore, DNA purification from samples could be omitted.
authors.affiliation: Department of Microbiology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima, 960-1295, Japan.
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Kaneko
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawana
authors.forename: Eiko
authors.initials: E
authors.lastname: Fukushima
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suzutani
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003470:Culture Media; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods* / Q000706:statistics & numerical data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods* / Q000706:statistics & numerical data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014709:Vero Cells
chemical_list: D003470:Culture Media; D004279:DNA, Viral