Cullin 5 gene expression in the rat cerebral cortex and hippocampus following traumatic brain injury (TBI).
Cullin-5 (Cul-5), a member of the cullin gene family of scaffold proteins of E3 ubiquitin-ligase complexes, has a role in proteolysis and cell cycle regulation. We recently demonstrated that cul-5 mRNA is ubiquitously expressed in the central nervous system. The present study used quantitative real time polymerase chain reaction and western blotting to measure changes in cul-5 mRNA and Cul-5 protein expression, respectively, in the injured CNS in response to traumatic brain injury (TBI). cul-5 mRNA levels were significantly decreased in the ipsilateral rat cerebral cortex on Days 1 and 7, but not on Day 3 following TBI. In the ipsilateral hippocampus, cul-5 mRNA was significantly reduced on Day 1 after TBI. Cul-5 protein levels were significantly decreased in the ipsilateral rat cerebral cortex on Days 1-7 post-TBI while levels were significantly lower in the ipsilateral hippocampus on Days 3-7 post-TBI. Since Cul-5 is ubiquitously expressed in eukaryotic cells and is linked to proteasome-mediated protein degradation, it may have a role in CNS cell fate determination under conditions of traumatic stress.
Yao
Xiang-Lan
XL
Graduate Program in Neuroscience, F. Edmund Hérbert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814-4799, USA. XYao@usuhs.mil
Liu
Jiong
J
Lee
Eleanor
E
Ling
Geoffrey S F
GS
McCabe
Joseph T
JT
D000818:Animals; D015153:Blotting, Western; D001930:Brain Injuries / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D044842:Cullin Proteins / Q000096:biosynthesis / Q000235:genetics*; D017931:DNA Primers; D015536:Down-Regulation / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017483:Receptors, Vasopressin / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
C094547:Cul5 protein, rat; D044842:Cullin Proteins; D017931:DNA Primers; D012333:RNA, Messenger; D017483:Receptors, Vasopressin; D044767:Ubiquitin-Protein Ligases