Characterization of bovine seminal plasma by proteomics.
Previous investigations of bovine seminal plasma (BSP) have revealed the identities of the three major proteins, BSP-PDC109, BSP-A3 and BSP-30 kDa, which together constitute about half of the total protein, as well as about 30 of the minor proteins. Analyses of BSP by 2-DE have revealed about 250 protein spots, suggesting that much of the BSP proteome remains undescribed. In this study, BSP has been analyzed by 2-D LC-based and SDS-PAGE-based proteomic methods. Ninety-nine proteins were identified, including 49 minor proteins that have not previously been described in seminal plasma of any species.
Kelly
Van C
VC
School of Biological Sciences, University of Auckland, Auckland, New Zealand.
Kuy
Sulee
S
Palmer
David J
DJ
Xu
Zhenzhong
Z
Davis
Stephen R
SR
Cooper
Garth J
GJ
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D016208:Databases, Factual; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008970:Molecular Weight; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000737:chemistry* / Q000378:metabolism; D013194:Staining and Labeling
D020543:Proteome; D029589:Seminal Plasma Proteins