Interference of Salmonella enteritidis and Lactobacillus spp. with IL-8 levels and transepithelial electrical resistance of enterocyte-like Caco-2 cells.
Caco-2 cells (exhibiting characteristics of mature villus enterocytes) were used to determine bacteria (Salmonella enteritidis causing human gastroenteritis)-intestinal cell interactions. The interference of bacteria with the transepithelial electrical resistance (TEER) of filter-grown Caco-2 cells and the production of IL-8 after exposure of the cells to S. enteritidis 857 and/or Lactobacillus strains (L. gasseri LF221 and L. rhamnosus BGT10) was evaluated. The strain 857 decreased TEER of filter-grown Caco-2 cells; in contrast, lactobacilli had a little or no effect. The effect of S. enteritidis on the TEER decreased if Caco-2 cells were pre-incubated with lactobacilli. This strain induced high levels of IL-8 (which can lead to cell damage). Compared to the IL-8 synthesis after exposure of Caco-2 cells to S. enteritidis 857, simultaneous exposure of Caco-2 cells to S. enteritidis and lactobacilli inhibited the IL-8 synthesis after short recovery periods.
Fajdiga
S
S
Zootechnical Department, Biotechnical Faculty, University of Ljubljana, Slovenia.
Koninkx
J F J G
JF
Tooten
P C J
PC
Marinsek-Logar
R
R
D018938:Caco-2 Cells / Q000378:metabolism / Q000382:microbiology; D017097:Electric Impedance; D006801:Humans; D018928:Immunity, Mucosal; D016209:Interleukin-8 / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology*; D007778:Lactobacillus / Q000502:physiology*; D012480:Salmonella Infections / Q000276:immunology*; D012477:Salmonella enteritidis / Q000472:pathogenicity*
D016209:Interleukin-8