Targeted deletion of the uvrBA operon and biological containment in the industrially important Bacillus licheniformis.
From a Bacillus licheniformis wild type as well as a defined asporogenous derivative, stable UV hypersensitive mutants were generated by targeted deletion of the uvrBA operon, encoding highly conserved key components of the nucleotide excision repair. Comparative studies, which included the respective parental strains, revealed no negative side effects of the deletion, neither on enzyme secretion nor on vegetative propagation. Thus, the uvrBA locus proved to be a useful deletion target for achieving biological containment in this industrially exploited bacterium. In contrast to recA mutants, which also display UV hypersensitivity, further strain development via homologous recombination techniques will be still possible in such uvr mutants.
Waldeck
Jens
J
Westfälische Wilhelms-Universität Münster, Institut für Molekulare Mikrobiologie und Biotechnologie, Corrensstrasse 3, 48149, Münster, Germany.
Meyer-Rammes
Heike
H
Nahrstedt
Hannes
H
Eichstädt
Renée
R
Wieland
Susanne
S
Meinhardt
Friedhelm
F
D001407:Bacillus / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D015139:Blotting, Southern; D017353:Gene Deletion; D005798:Genes, Bacterial / Q000235:genetics*; D007218:Industrial Microbiology / Q000379:methods; D008957:Models, Genetic; D009154:Mutation; D009876:Operon / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013014:SOS Response, Genetics; D014466:Ultraviolet Rays