Construction of plasmids for RNA interference and in vitro transcription of double-stranded RNA.
Double-stranded RNA (dsRNA)-induced gene silencing in Caenorhabditis elegans involves the manufacture and delivery of defined sequences of dsRNA to the organism, followed by a careful monitoring for loss-of-function phenocopies in treated animals. In this chapter, we describe how to generate DNAs that can be used as templates for transcription of dsRNA.
Timmons
Lisa
L
Department of Molecular Biosciences, University of Kansas, Lawrence, USA.
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D002474:Cell-Free System; D017238:Genes, Helminth / Q000235:genetics*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000494:pharmacology; D014158:Transcription, Genetic
D012330:RNA, Double-Stranded