A pragmatic and readily implemented quality control strategy for HPLC-MS and GC-MS-based metabonomic analysis.
Metabonomic/metabolomic studies can involve the analysis of large numbers of samples for the detection of biomarkers and confidence in the analytical data, generated by methods such as GC and HPLC-MS, requires active measures on the part of the analyst. However, quality control for complex multi-component samples such as biofluids, where many of the components of interest in the sample are unknown prior to analysis, poses significant problems. Here the repeat analysis of a pooled sample throughout the run, thereby enabling the analysis to be monitored and controlled using targeted inspection of the data and pattern recognition, is advocated as a pragmatic solution to this problem.
Sangster
Timothy
T
Department of Drug Metabolism and Pharmacokinetics, AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire, UK SK10 4TG.
Major
Hilary
H
Plumb
Robert
R
Wilson
Amy J
AJ
Wilson
Ian D
ID
D015415:Biomarkers / Q000032:analysis; D001826:Body Fluids / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D011786:Quality Control; D014020:Tissue Extracts / Q000737:chemistry / Q000378:metabolism
D015415:Biomarkers; D014020:Tissue Extracts