title: Improved glucuronide hydrolysis in the determination of urinary 1-hydroxypyrene.
abstract: The efficiency of the glucuronide hydrolysis in the determination of urinary 1-hydroxypyrene was investigated as a function of the reaction conditions. A significant improvement could be obtained by increasing the enzyme concentration described in the literature.
authors.affiliation: Vrije Universiteit Amsterdam, Institute for Environmnetal Studies, De Boelelaan 1087, NL-1081HV Amsterdam, Netherlands. jan.willem.wegener@ivm.vu.nl
authors.forename: J W M
authors.initials: JW
authors.lastname: Wegener
authors.forename: G H J
authors.initials: GH
authors.lastname: Hopman-Ubbels
authors.forename: M
authors.initials: M
authors.lastname: Van Velzen
mesh_terms: D005966:Glucuronidase / Q000378:metabolism; D020719:Glucuronides / Q000378:metabolism* / Q000652:urine*; D006801:Humans; D006868:Hydrolysis; D011721:Pyrenes / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D020719:Glucuronides; D011721:Pyrenes; D005966:Glucuronidase; C033146:1-hydroxypyrene