title: Preclinical model to test human papillomavirus virus (HPV) capsid vaccines in vivo using infectious HPV/cottontail rabbit papillomavirus chimeric papillomavirus particles.
abstract: A human papillomavirus (HPV) vaccine consisting of virus-like particles (VLPs) was recently approved for human use. It is generally assumed that VLP vaccines protect by inducing type-specific neutralizing antibodies. Preclinical animal models cannot be used to test for protection against HPV infections due to species restriction. We developed a model using chimeric HPV capsid/cottontail rabbit papillomavirus (CRPV) genome particles to permit the direct testing of HPV VLP vaccines in rabbits. Animals vaccinated with CRPV, HPV type 16 (HPV-16), or HPV-11 VLPs were challenged with both homologous (CRPV capsid) and chimeric (HPV-16 capsid) particles. Strong type-specific protection was observed, demonstrating the potential application of this approach.
authors.affiliation: Gittlen Cancer Research Foundation, Pennsylvania State University College of Medicine, 500 University Dr., Hershey, PA 17033-2390, USA.
authors.forename: Andres F
authors.initials: AF
authors.lastname: Mejia
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Culp
authors.forename: Nancy M
authors.initials: NM
authors.lastname: Cladel
authors.forename: Karla K
authors.initials: KK
authors.lastname: Balogh
authors.forename: Lynn R
authors.initials: LR
authors.lastname: Budgeon
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Buck
authors.forename: Neil D
authors.initials: ND
authors.lastname: Christensen
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000276:immunology*; D012777:Cottontail rabbit papillomavirus / Q000276:immunology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D052140:Human papillomavirus 11 / Q000276:immunology*; D052162:Human papillomavirus 16 / Q000276:immunology*; D030361:Papillomavirus Infections / Q000473:pathology / Q000517:prevention & control*; D053918:Papillomavirus Vaccines / Q000276:immunology*; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014771:Virion / Q000276:immunology*
chemical_list: D036022:Capsid Proteins; D053918:Papillomavirus Vaccines; D011993:Recombinant Fusion Proteins