title: New screening method for basic compounds in urine by on-line extraction-high-performance liquid chromatography with photodiode-array detection.
abstract: A fully automated, qualitative screening HPLC method for the identification of basic compounds in urine has been developed. A 1-ml volume of urine was extracted by on-line extraction and separated on two coupled strong cation-exchange (SCX) columns (2 x LunaSCX, 150 mm x 4.6 mm, 5 microm) under isocratic conditions. The mobile phase consisted of a mixture of potassium dihydrogenphosphate buffer (pH 2.3) and acetonitrile. The use of photodiode-array detection (DAD, lambda = 190-800 nm) gave access to a library of approximately 2600 toxicologically relevant compounds. The validated method is reliable, simple and in addition successfully proven with the analysis of real biological specimen for the routine use.
authors.affiliation: Berliner Betrieb fuer Zentrale Gesundheitliche Aufgaben (BBGes), Institute of Clinical Toxicology-Clinical Toxicology and Poison Control Center, Oranienburgerstrasse 285, 13437 Berlin, Germany.
authors.forename: Lena
authors.initials: L
authors.lastname: Schönberg
authors.forename: Thomas
authors.initials: T
authors.lastname: Grobosch
authors.forename: Dagmar
authors.initials: D
authors.lastname: Lampe
authors.forename: Charlotte
authors.initials: C
authors.lastname: Kloft
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D000662:Amphetamines / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009862:Online Systems; D018847:Opioid Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D015203:Reproducibility of Results
chemical_list: D000470:Alkaloids; D000662:Amphetamines; D018847:Opioid Peptides