title: Identification of pentadecapeptide mimicking muramyl peptide.
abstract: We used monoclonal antibody, generated against N-acetylglucosaminyl-beta1-4-N-acetylmuramyl-alanyl-D-isoglutamine (GMDP), and phage display libraries of random peptides to select for oligopeptides, that mimic GMDP in their biological activity. Selected phage clones displayed a peptide RVPPRYHAKISPMVN (called RN-peptide) on their surface. This peptide was synthesized. RN-peptide was shown to augment the antibody response to ovalbumin in mice while the peptide was non-immunogenic and non-pyrogenic. We also characterized adjuvant activity of 14-, 10- and 7-mer analogs of RN-peptide truncated at the C-terminus and found them to be active. Because both carbohydrate and peptide fragments are critical for the biological activity of muramyl peptides, the results indicate that RN-peptide mimicks the spatial structure of intact GMDP.
authors.affiliation: Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospekt Nauki 6, Puschino, Moscow Region 142290, Russia.
authors.forename: Alexander Georgievich
authors.initials: AG
authors.lastname: Laman
authors.forename: Anna Olegovna
authors.initials: AO
authors.lastname: Shepelyakovskaya
authors.forename: Igor Anatolevich
authors.initials: IA
authors.lastname: Berezin
authors.forename: Khanafi Magometovich
authors.initials: KM
authors.lastname: Boziev
authors.forename: Igor Leonidovich
authors.initials: IL
authors.lastname: Rodionov
authors.forename: Irina Alexandrovna
authors.initials: IA
authors.lastname: Chulina
authors.forename: Galina Vladimirovna
authors.initials: GV
authors.lastname: Malakhova
authors.forename: Fedor Alexandrovich
authors.initials: FA
authors.lastname: Brovko
authors.forename: Arkadi Nikolaevich
authors.initials: AN
authors.lastname: Murashev
authors.forename: Timo Kalevi
authors.initials: TK
authors.lastname: Korpela
authors.forename: Vladimir Andreevich
authors.initials: VA
authors.lastname: Nesmeyanov
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology*; D019151:Peptide Library; D011749:Pyrogens / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D009842:Oligopeptides; D019151:Peptide Library; D011749:Pyrogens; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C023868:N-acetyl-beta-glucosaminyl-N-acetylmuramyl-alanylisoglutamine