title: Molecular weights from approach-to-sedimentation equilibrium data using nonlinear regression analysis.
abstract: Data obtained from early times during the transient period of sedimentation equilibrium experiments are analyzed using an approximate solution to the Lamm equation to estimate s/D. The Cr versus r data obtained at several times during approach-to-equilibrium are analyzed using a nonlinear least squares algorithm and Fujita's approximate solution. This procedure was tested using D-Ser13-somatostatin, ribonuclease, and ovalbumin. The results obtained demonstrate that for monodisperse samples s/D may be rapidly and reliably estimated using this method.
authors.affiliation: Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Holladay