title: Electroenzymatic polypyrrole-intercalator sensor for the determination of West Nile virus cDNA.
abstract: The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
authors.forename: Rodica E
authors.initials: RE
authors.lastname: Ionescu
authors.forename: Sebastien
authors.initials: S
authors.lastname: Herrmann
authors.forename: Laurent
authors.initials: L
authors.lastname: Bouffier
authors.forename: Martine
authors.initials: M
authors.lastname: Demeunynck
authors.forename: Robert S
authors.initials: RS
authors.lastname: Marks
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D014902:West Nile virus / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole