title: Carotid arterial stiffness, elastic fibre network and vasoreactivity in semicarbazide-sensitive amine-oxidase null mouse.
abstract: OBJECTIVE
  We examined the arterial phenotype of semicarbazide-sensitive amine-oxidase null mouse (SSAO -/-) using various techniques including high resolution echotracking.
  
  
  RESULTS
  SSAO -/- mice showed no change in arterial pressure under anesthesia. The in vivo arterial diameter, only measured in the carotid artery (CA), was higher in SSAO -/- than in SSAO +/+ animals. Elastic modulus-wall stress curves and CA rupture pressure were similar between SSAO -/- and +/+ mice, indicating no change in arterial wall stiffness or mechanical strength. There was no significant difference in insoluble elastin, total collagen content and elastic lamellar morphology between the two genotypes. No alteration in vascular reactivity was observed in aortic rings and mesenteric arteries from SSAO -/- mice. Aortic lysyl oxidase (LO) activity remained unaltered, indicating that SSAO invalidation is not accompanied by a compensatory increase in LO activity.
  
  
  CONCLUSIONS
  This is the first functional study of arteries lacking SSAO. Our results indicate that SSAO -/- mice present an increased arterial diameter associated with normal arterial mechanical properties, suggesting that SSAO deficiency might contribute to arterial wall remodeling. However, these results argue against the hypothesis that SSAO intervenes in elastic fibre organization, elastin cross-linking processes and vasoreactivity.
authors.affiliation: INSERM U684, Vandoeuvre-les-Nancy, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Mercier
authors.forename: Mary
authors.initials: M
authors.lastname: Osborne-Pellegrin
authors.forename: Khadija
authors.initials: K
authors.lastname: El Hadri
authors.forename: Augustine
authors.initials: A
authors.lastname: Kakou
authors.forename: Carlos
authors.initials: C
authors.lastname: Labat
authors.forename: Laurent
authors.initials: L
authors.lastname: Loufrani
authors.forename: Daniel
authors.initials: D
authors.lastname: Henrion
authors.forename: Pascal
authors.initials: P
authors.lastname: Challande
authors.forename: Sirpa
authors.initials: S
authors.lastname: Jalkanen
authors.forename: Bruno
authors.initials: B
authors.lastname: Fève
authors.forename: Patrick
authors.initials: P
authors.lastname: Lacolley
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001011:Aorta; D015153:Blotting, Western / Q000379:methods; D017536:Carotid Artery, Common / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D003094:Collagen / Q000032:analysis; D004547:Elastic Tissue / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D004548:Elasticity; D004549:Elastin / Q000032:analysis; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008297:Male; D008638:Mesenteric Arteries / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D008249:Protein-Lysine 6-Oxidase / Q000032:analysis; D033081:Shear Strength; D014666:Vasomotor System / Q000502:physiology
chemical_list: D003094:Collagen; D004549:Elastin; D008249:Protein-Lysine 6-Oxidase; D006631:Amine Oxidase (Copper-Containing)