title: Cryopreservation of cultivated and wild potato varieties by droplet vitrification: effect of subculture of mother-plants and of preculture of shoot tips.
abstract: In this paper, we studied the effect of subculture of mother-plants and of preculture of shoot tips of two potato varieties (Dejima, cultivated and STN13, wild) cryopreserved using the droplet-vitrification technique. The subculture conditions (light intensity, aeration and planting density) significantly affected survival of both non-cryopreserved and cryopreserved shoot-tips in both varieties. The subculture duration and the position of the shoot tips on the axis of the in vitro plantlets had a significant (P<0.0001) effect on survival of cryopreserved shoot tips. The optimal subculture duration was 7 and 5 weeks and the optimal size of shoot tips was 1.5-2.0 and 1.0-1.5 mm for var. Dejima and STN13, respectively. Survival of cryopreserved shoot tips was influenced by the sucrose concentration in the preculture medium and the preculture duration. The highest survival of cryopreserved shoot tips was observed after preculture with 0.3 M sucrose for 8 h followed by 0.7 M sucrose for 18 h. These results indicate that the parameters of the subculture of mother-plants and of preculture of shoot tips should be carefully optimized, especially in the case of wild species.
authors.affiliation: National Institute of Agricultural Biotechnology, RDA, Suwon, Korea.
authors.forename: Ju-Won
authors.initials: JW
authors.lastname: Yoon
authors.forename: Haeng-Hoon
authors.initials: HH
authors.lastname: Kim
authors.forename: Ho-Cheol
authors.initials: HC
authors.lastname: Ko
authors.forename: Hae-Sung
authors.initials: HS
authors.lastname: Hwang
authors.forename: Eun-Sun
authors.initials: ES
authors.lastname: Hong
authors.forename: Eun-Gi
authors.initials: EG
authors.lastname: Cho
authors.forename: Florent
authors.initials: F
authors.lastname: Engelmann
mesh_terms: D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods; D004305:Dose-Response Relationship, Drug; D018520:Plant Shoots / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D032322:Solanum / Q000166:cytology / Q000235:genetics* / Q000502:physiology*; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors
chemical_list: D003451:Cryoprotective Agents; D003470:Culture Media; D013395:Sucrose