Single-particle evanescent light scattering simulations for total internal reflection microscopy.
We simulate and measure light scattering of a micrometer-sized spherical particle suspended in solution close to a glass substrate. The model, based on the discrete sources method, is developed to describe the experimental situation of total internal reflection microscopy experiments; i.e., the particle is illuminated by an evanescent light field originating from the glass-solvent interface. In contrast to the well-established assumption of a simple exponential decay of the scattering intensity with distance, we demonstrate significant deviations for a certain range of penetration depths and polarization states of the incident light.
Helden
Laurent
L
Physikalisches Institut, Universität Stuttgart, Stuttgart, Germany. L.Helden@physik.uni-stuttgart.de
Eremina
Elena
E
Riefler
Norbert
N
Hertlein
Christopher
C
Bechinger
Clemens
C
Eremin
Yuri
Y
Wriedt
Thomas
T
D000465:Algorithms; D003102:Colloids / Q000737:chemistry*; D003198:Computer Simulation; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008027:Light; D008853:Microscopy / Q000379:methods*; D008956:Models, Chemical; D010316:Particle Size; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity
D003102:Colloids