title: Insertion/deletion polymorphism on ACE gene is associated with endothelial dysfunction in young patients with hypertension.
abstract: Endothelial dysfunction, insulin resistance (IR) and genetic predispositions are important risk factors of hypertension. Aim of our study was to test the hypothesis, whether insertion/deletion (I/D) polymorphism on the angiotensin converting enzyme (ACE) gene and M235T polymorphism on angiotesinogen gene (AGT) correlates with parameters of insulin sensitivity and plasminogen activator inhibitor (PAI-1) levels in newly diagnosed hypertensive patients as compared with normotensive controls. Blood pressure (BP), fasting plasma glucose, insulin, epinephrine, norepinephrine and PAI-1 concentrations were determined in 30 male patients with hypertension grade 1 (HT) and in 31 matched healthy subjects (NT). Insulin resistance was estimated using IR HOMA formula. Patients with HT had increased levels of PAI-1, norepinephrine, fasting plasma insulin levels, IR HOMA (p<0.001) compared to controls. Subjects (HT and NT) with DD and ID genotype had a significantly higher systolic BP (p<0.05) and PAI-1 compared to those with II genotype. Homozygous subjects 235T had a higher systolic BP and higher levels of epinephrine and norepinephrine than heterozygous or homozygous M235 (p<0.05). In conclusion, no association was found between M235T polymorphism and insulin resistance or PAI-1 levels, but results indicate relationship between I/D polymorphism of the ACE gene and plasma PAI-1 levels in the early stage of hypertension.
authors.affiliation: Institute of Experimental Endocrinology Slovak Academy of Sciences, Bratislava, Slovakia. Adela.Penesova@savba.sk
authors.forename: A
authors.initials: A
authors.lastname: Penesova
authors.forename: E
authors.initials: E
authors.lastname: Cizmarova
authors.forename: R
authors.initials: R
authors.lastname: Kvetnansky
authors.forename: J
authors.initials: J
authors.lastname: Koska
authors.forename: B
authors.initials: B
authors.lastname: Sedlakova
authors.forename: O
authors.initials: O
authors.lastname: Krizanova
mesh_terms: D016022:Case-Control Studies; D004727:Endothelium / Q000201:enzymology / Q000503:physiopathology*; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology*; D008297:Male; D009154:Mutation / Q000235:genetics*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: D007703:Peptidyl-Dipeptidase A