title: Reversal of diet-induced insulin resistance with a single bout of exercise in the rat: the role of PTP1B and IRS-1 serine phosphorylation.
abstract: Lifestyle interventions including exercise programmes are cornerstones in the prevention of obesity-related diabetes. In this study, we demonstrate that a single bout of exercise inhibits high-fat diet-induced insulin resistance. Diet-induced obesity (DIO) increased the expression and activity of the protein tyrosine phosphatase 1B (PTP1B) and attenuated insulin signalling in gastrocnemius muscle of rats, a phenomenon which was reversed by a single session of exercise. In addition, DIO was observed to lead to serine phosphorylation of insulin receptor substrate 1 (IRS-1), which was also reversed by exercise in muscle in parallel with a reduction in c-Jun N-terminal kinase (JNK) activity. Thus, acute exercise increased the insulin sensitivity during high-fat feeding in obese rats. Overall, these results provide new insights into the mechanism by which exercise restores insulin sensitivity.
authors.affiliation: Departamento de Clínica, Médica, FCM-UNICAMP, Cidade Universitária Zeferino Vaz, Campinas, São Paulo, Brazil, 13081-970.
authors.forename: Eduardo R
authors.initials: ER
authors.lastname: Ropelle
authors.forename: José R
authors.initials: JR
authors.lastname: Pauli
authors.forename: Patrícia O
authors.initials: PO
authors.lastname: Prada
authors.forename: Cláudio T
authors.initials: CT
authors.lastname: de Souza
authors.forename: Paty K
authors.initials: PK
authors.lastname: Picardi
authors.forename: Marcel C
authors.initials: MC
authors.lastname: Faria
authors.forename: Dennys E
authors.initials: DE
authors.lastname: Cintra
authors.forename: Maria Fernanda de A
authors.initials: MF
authors.lastname: Fernandes
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: Flores
authors.forename: Lício A
authors.initials: LA
authors.lastname: Velloso
authors.forename: Mario J A
authors.initials: MJ
authors.lastname: Saad
authors.forename: José B C
authors.initials: JB
authors.lastname: Carvalheira
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D020672:I-kappa B Proteins / Q000037:antagonists & inhibitors; D007328:Insulin / Q000378:metabolism; D055504:Insulin Receptor Substrate Proteins; D007333:Insulin Resistance; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D009765:Obesity / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism*; D010766:Phosphorylation; D010805:Physical Conditioning, Animal; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D012694:Serine / Q000378:metabolism; D015398:Signal Transduction; D013550:Swimming
chemical_list: D004041:Dietary Fats; D020672:I-kappa B Proteins; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D010750:Phosphoproteins; D012694:Serine; D048031:JNK Mitogen-Activated Protein Kinases; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D017027:Protein Tyrosine Phosphatases; C516610:Ptpn1 protein, rat