Inhibition of influenza virus infection by a novel antiviral peptide that targets viral attachment to cells.
Influenza A viruses continue to cause widespread morbidity and mortality. There is an added concern that the highly pathogenic H5N1 influenza A viruses, currently found throughout many parts of the world, represent a serious public health threat and may result in a pandemic. Intervention strategies to halt an influenza epidemic or pandemic are a high priority, with an emphasis on vaccines and antiviral drugs. In these studies, we demonstrate that a 20-amino-acid peptide (EB, for entry blocker) derived from the signal sequence of fibroblast growth factor 4 exhibits broad-spectrum antiviral activity against influenza viruses including the H5N1 subtype in vitro. The EB peptide was protective in vivo, even when administered postinfection. Mechanistically, the EB peptide inhibits the attachment to the cellular receptor, preventing infection. Further studies demonstrated that the EB peptide specifically binds to the viral hemagglutinin protein. This novel peptide has potential value as a reagent to study virus attachment and as a future therapeutic.
Jones
Jeremy C
JC
Department of Medical Microbiology and Immunology, 1300 University Avenue, Room 417 SMI, Madison, WI 53706, USA.
Turpin
Elizabeth A
EA
Bultmann
Hermann
H
Brandt
Curtis R
CR
Schultz-Cherry
Stacey
S
D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002460:Cell Line; D004285:Dogs; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D009980:Influenza A virus / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000188:drug therapy / Q000517:prevention & control*; D010455:Peptides / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D019444:Vaccines, DNA; D053585:Virus Attachment / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D010455:Peptides; D019444:Vaccines, DNA