title: Ubiquitin-binding motifs in REV1 protein are required for its role in the tolerance of DNA damage.
abstract: REV1 protein is a eukaryotic member of the Y family of DNA polymerases involved in the tolerance of DNA damage by replicative bypass. The precise role(s) of REV1 in this process is not known. Here we show, by using the yeast two-hybrid assay and the glutathione S-transferase pull-down assay, that mouse REV1 can physically interact with ubiquitin. The association of REV1 with ubiquitin requires the ubiquitin-binding motifs (UBMs) located at the C terminus of REV1. The UBMs also mediate the enhanced association between monoubiquitylated PCNA and REV1. In cells exposed to UV radiation, the association of REV1 with replication foci is dependent on functional UBMs. The UBMs of REV1 are shown to contribute to DNA damage tolerance and damage-induced mutagenesis in vivo.
authors.affiliation: Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9072, USA.
authors.forename: Caixia
authors.initials: C
authors.lastname: Guo
authors.forename: Tie-Shan
authors.initials: TS
authors.lastname: Tang
authors.forename: Marzena
authors.initials: M
authors.lastname: Bienko
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Parker
authors.forename: Aleksandra B
authors.initials: AB
authors.lastname: Bielen
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Sonoda
authors.forename: Shunichi
authors.initials: S
authors.lastname: Takeda
authors.forename: Helle D
authors.initials: HD
authors.lastname: Ulrich
authors.forename: Ivan
authors.initials: I
authors.lastname: Dikic
authors.forename: Errol C
authors.initials: EC
authors.lastname: Friedberg
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002645:Chickens; D002522:Chlorocebus aethiops; D004249:DNA Damage; D004259:DNA-Directed DNA Polymerase; D005982:Glutathione Transferase / Q000378:metabolism; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques; D025801:Ubiquitin / Q000378:metabolism; D014466:Ultraviolet Rays; D000099270:Y-Family DNA Polymerases
chemical_list: D004259:DNA-Directed DNA Polymerase; D005982:Glutathione Transferase; D009713:Nucleotidyltransferases; D011993:Recombinant Fusion Proteins; D025801:Ubiquitin; C496086:Rev1 protein, mouse; D000099270:Y-Family DNA Polymerases