Nanoparticle probes with surface enhanced Raman spectroscopic tags for cellular cancer targeting.
We have developed biocompatible, photostable, and multiplexing-compatible surface-enhanced Raman spectroscopic tagging material (SERS dots) composed of silver nanoparticle-embedded silica spheres and organic Raman labels for cellular cancer targeting in living cells. SERS dots showed linear dependency of Raman signatures on their different amounts, allowing their possibility for the quantification of targets. In addition, the antibody-conjugated SERS dots were successfully applied to the targeting of HER2 and CD10 on cellular membranes and exhibited good specificity. SERS dots demonstrate the potential for high-throughput screening of biomolecules using vibrational information.
Kim
Jong-Ho
JH
School of Chemical and Biological Engineering, College of Veterinary Medicine, Department of Chemistry Education, Seoul National University, Seoul 151-747, Korea.
Kim
Jun-Sung
JS
Choi
Heejeong
H
Lee
Sang-Myung
SM
Jun
Bong-Hyun
BH
Yu
Kyeong-Nam
KN
Kuk
Eunye
E
Kim
Yong-Kweon
YK
Jeong
Dae Hong
DH
Cho
Myung-Haing
MH
Lee
Yoon-Sik
YS
D006801:Humans; D053768:Metal Nanoparticles; D009369:Neoplasms / Q000473:pathology*; D013059:Spectrum Analysis, Raman / Q000379:methods*