title: The RPS adeno detector for diagnosing adenoviral conjunctivitis.
abstract: OBJECTIVE
  To compare the sensitivity, specificity, and accuracy of the RPS Adeno Detector (Rapid Pathogen Screening Inc., South Williamsport, PA) against both viral cell culture with confirmatory immunofluorescence staining (CC-IFA) and the polymerase chain reaction (PCR) for diagnosing adenoviral conjunctivitis.
  
  
  METHODS
  Prospective, nonrandomized, masked, multicenter clinical trial.
  
  
  METHODS
  One hundred eighty-six consecutive patients from 5 clinical centers seeking treatment within 1 week of developing a red eye and thought to have acute conjunctivitis.
  
  
  METHODS
  The RPS Adeno Detector is a 10-minute in-office lateral flow immunoassay. Patients were tested with the RPS Adeno Detector, CC-IFA, and PCR to detect the presence of adenovirus.
  
  
  METHODS
  The sensitivity, specificity, and accuracy of the RPS Adeno Detector were assessed for identifying cases of adenoviral conjunctivitis.
  
  
  RESULTS
  Compared with CC-IFA, the RPS Adeno Detector was 88% sensitive and 91% specific at detecting adenoviral conjunctivitis. Using PCR as a reference method, the sensitivity of the RPS Adeno Detector increased to 89% and the specificity increased to 94%. Compared with PCR, CC-IFA was found to be 91% as sensitive and 100% as specific.
  
  
  CONCLUSIONS
  The RPS Adeno Detector demonstrated sufficient sensitivity and specificity to be used in the physician's office for the detection of adenoviral conjunctivitis.
authors.affiliation: Manatee Sarasota Eye Clinic & Laser Center, Bradenton, Florida, USA. robsambo@yahoo.com
authors.forename: Robert
authors.initials: R
authors.lastname: Sambursky
authors.forename: Shachar
authors.initials: S
authors.lastname: Tauber
authors.forename: Frank
authors.initials: F
authors.lastname: Schirra
authors.forename: Kristian
authors.initials: K
authors.lastname: Kozich
authors.forename: Richard
authors.initials: R
authors.lastname: Davidson
authors.forename: Elisabeth J
authors.initials: EJ
authors.lastname: Cohen
mesh_terms: D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000175:diagnosis* / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D003236:Conjunctivitis, Viral / Q000175:diagnosis* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D007223:Infant; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013666:Tears / Q000821:virology; D014776:Virus Cultivation
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic