title: Vaccination of bovines with recombinant Boophilus Yolk pro-Cathepsin.
abstract: Boophilus Yolk pro-Cathepsin (BYC) is an aspartic proteinase found in Boophilus microplus eggs that is involved in the embryogenesis and has been tested as antigen to compose an anti-tick vaccine. The vaccine potential of a recombinant BYC expressed in Escherichia coli (rBYC) was investigated. rBYC was purified and used to immunize Hereford cattle. The sera of bovines immunized with rBYC recognized the native BYC with a titer ranging from 125 to 4000. Furthermore, immunized bovines challenged with 20,000 larvae presented an overall protection of 25.24%. The partial protection obtained against B. microplus infestation with the recombinant protein immunization was similar to the already described for native BYC immunization.
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500, Prédio 43421, Porto Alegre RS 91501-970, Brazil.
authors.forename: Alexandre T
authors.initials: AT
authors.lastname: Leal
authors.forename: Adriana
authors.initials: A
authors.lastname: Seixas
authors.forename: Paula C
authors.initials: PC
authors.lastname: Pohl
authors.forename: Carlos A S
authors.initials: CA
authors.lastname: Ferreira
authors.forename: Carlos
authors.initials: C
authors.lastname: Logullo
authors.forename: Pedro L
authors.initials: PL
authors.lastname: Oliveira
authors.forename: Sandra E
authors.initials: SE
authors.lastname: Farias
authors.forename: Carlos
authors.initials: C
authors.lastname: Termignoni
authors.forename: Itabajara
authors.initials: I
authors.lastname: da Silva Vaz
authors.forename: Aoi
authors.initials: A
authors.lastname: Masuda
mesh_terms: D000818:Animals; D000917:Antibody Formation; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control*; D004792:Enzyme Precursors / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D026863:Ixodidae / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013984:Tick Infestations / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control* / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary*
chemical_list: D004792:Enzyme Precursors; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D016282:Aspartic Acid Endopeptidases; C113293:Boophilus yolk pro-cathepsin