Single nucleotide polymorphism in the cytolethal distending toxin B gene confers heterogeneity in the cytotoxicity of Actinobacillus actinomycetemcomitans.
Clinical Actinobacillus actinomycetemcomitans produces cytolethal distending toxin (CDT) with titers ranging from 10(2) to 10(8) U/mg. Single nucleotide polymorphism analysis of the cdt gene in clinical isolates identified a variation of a single amino acid at residue 281 of CdtB, which significantly affected CDT toxicity by modulating the chromatin-degrading activity of CdtB.
Nishikubo
Shuichi
S
Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8553, Japan.
Ohara
Masaru
M
Ikura
Masae
M
Katayanagi
Katsuo
K
Fujiwara
Tamaki
T
Komatsuzawa
Hitoshi
H
Kurihara
Hidemi
H
Sugai
Motoyuki
M
D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001427:Bacterial Toxins / Q000235:genetics* / Q000633:toxicity*; D002843:Chromatin / Q000187:drug effects; D005798:Genes, Bacterial; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D020641:Polymorphism, Single Nucleotide; D037521:Virulence Factors / Q000235:genetics* / Q000633:toxicity*
D001427:Bacterial Toxins; D002843:Chromatin; D037521:Virulence Factors; C067044:cytolethal distending toxin