title: Transcription inhibition using oligonucleotide-modified gold nanoparticles.
abstract: The capture of T7 RNA polymerase using double-stranded promoter DNA on the surface of gold nanoparticles has been demonstrated. The competitive binding and inhibition of T7 RNA polymerase due to specific interactions on the nanoparticle surface represents a transcription factor decoy approach in a model system. The efficiency of inhibition was determined for various nanoparticle sizes, surface coverage, and linker length for double-stranded promoter DNA on gold nanoparticles. The experiments provide a basis for determining the accessibility of binding sites on nanoparticle surfaces for applications involving cell targeting or the use of nanoparticles as binding agents in solution.
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, 27695, USA.
authors.forename: Chiamaka
authors.initials: C
authors.lastname: Agbasi-Porter
authors.forename: Jessica
authors.initials: J
authors.lastname: Ryman-Rasmussen
authors.forename: Stefan
authors.initials: S
authors.lastname: Franzen
authors.forename: Daniel
authors.initials: D
authors.lastname: Feldheim
mesh_terms: D002460:Cell Line; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D009841:Oligonucleotides; D014764:Viral Proteins; D006046:Gold; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases