Identification of a novel, invasive, not-yet-cultivated Treponema sp. in the large intestine of pigs by PCR amplification of the 16S rRNA gene.
Laser capture microdissection in combination with fluorescent in situ hybridization was used to identify an unknown species of spirochetes from the pig colonic mucosa. The 16S rRNA gene was PCR amplified, and the closest related type strain was Treponema bryantiiT (90.1%). The spirochete, here named "Candidatus Treponema suis," was associated with colitis, including invasion of the surface epithelium as well as superficial parts of the mucosa.
Mølbak
Lars
L
Danish Institute for Food and Veterinary Research, Bülowsvej 27, DK-1790 Copenhagen V, Denmark. lam@dfvf.dk
Klitgaard
Kirstine
K
Jensen
Tim K
TK
Fossi
Marja
M
Boye
Mette
M
D000818:Animals; D003092:Colitis / Q000382:microbiology / Q000662:veterinary; D003106:Colon / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D020459:Genes, rRNA; D017404:In Situ Hybridization, Fluorescence; D007413:Intestinal Mucosa / Q000382:microbiology; D042282:Microdissection / Q000379:methods; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000382:microbiology*; D014208:Treponema / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014211:Treponemal Infections / Q000382:microbiology / Q000662:veterinary*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S