title: Diet and gene expression: delta-5 and delta-6 desaturases in healthy Chinese and European subjects.
abstract: OBJECTIVE
  To compare the composition of fatty acids (FAs) in diet, and the expression of delta-6 desaturase (D6D) and delta-5 desaturase (D5D) genes in peripheral blood mononuclear cells (PBMCs) between Chinese and Europeans.
  
  
  METHODS
  Three-day dietary records from 20 subjects from Beijing, China (n = 10) and Kent, UK (n = 10) were analysed. Expression of PBMC D6D and D5D genes of the subjects was determined using RT-PCR.
  
  
  RESULTS
  The dietary intake of Chinese subjects contained less saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs), but more essential fatty acids (EFAs) than that of Europeans. Levels of expression of PBMC D6D and D5D genes of Chinese subjects were significantly lower than those of Europeans. A significant positive correlation was found between dietary intake of total SFAs and total MUFAs and expression of PBMC D6D and D5D genes, but a significant negative correlation between dietary intake of linoleic acid (LA) and alpha-linolenic acid (LNA) and the expression of PBMC D6D and D5D genes.
  
  
  CONCLUSIONS
  Intake of high SFAs and MUFAs appears to increase expression of PBMC D6D and D5D genes, whilst high EFAs intake appears to decrease expression of PBMC D6D and D5D genes. A follow-up study of the expression of D6D and D5D genes in Chinese who live in European countries with high SFA and MUFA diets would be of interest.
authors.affiliation: Centre for Biosciences Research, School of Science, University of Greenwich, Kent, UK.
authors.forename: M
authors.initials: M
authors.lastname: Xiang
authors.forename: M A
authors.initials: MA
authors.lastname: Rahman
authors.forename: H
authors.initials: H
authors.lastname: Ai
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: L S
authors.initials: LS
authors.lastname: Harbige
mesh_terms: D000328:Adult; D000886:Anthropometry; D044466:Asian People / Q000235:genetics*; D002681:China; D000090943:Delta-5 Fatty Acid Desaturase; D004032:Diet; D004040:Dietary Carbohydrates / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D004044:Dietary Proteins / Q000494:pharmacology; D005060:Europe; D044943:Fatty Acid Desaturases / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D005227:Fatty Acids / Q000494:pharmacology; D005228:Fatty Acids, Essential / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000201:enzymology*; D019787:Linoleic Acid / Q000494:pharmacology; D050602:Linoleoyl-CoA Desaturase / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044465:White People / Q000235:genetics*; D017962:alpha-Linolenic Acid / Q000494:pharmacology
chemical_list: D000090943:Delta-5 Fatty Acid Desaturase; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D004044:Dietary Proteins; D005227:Fatty Acids; D005228:Fatty Acids, Essential; D005231:Fatty Acids, Unsaturated; D012333:RNA, Messenger; D017962:alpha-Linolenic Acid; D019787:Linoleic Acid; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase