Mild hydrolysis of nitriles by Fusarium solani strain O1.
High levels of an aromatic nitrilase (about 37 microkat/L culture) were induced in Fusarium solani O1 after transfer of the mycelium from a rich medium into a medium with 20 mmol/L picolinonitrile. The mycelium was entrapped in lense-shaped particles consisting of a polyvinyl alcohol/polyethylene glycol copolymer (LentiKats). The cell-free extract was immobilized by hydrophobic binding onto a Butyl Sepharose column. The enzyme was useful for the mild hydrolysis of nicotinonitrile, isonicotinonitrile and benzonitrile.
Vejvoda
V
V
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czechia.
Kaplan
O
O
Klozová
J
J
Masák
J
J
Cejková
A
A
Jirků
V
V
Stloukal
R
R
Martínková
L
L
D000619:Aminohydrolases / Q000096:biosynthesis*; D018914:Cells, Immobilized / Q000378:metabolism / Q000382:microbiology; D005670:Fusarium / Q000201:enzymology* / Q000254:growth & development; D006868:Hydrolysis; D009570:Nitriles / Q000378:metabolism*
D009570:Nitriles; D000619:Aminohydrolases; C021867:nitrilase