title: First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value.
abstract: The EC 'Structural Proteomics In Europe' contract is aimed specifically at the atomic resolution structure determination of human protein targets closely linked to health, with a focus on cancer (kinesins, kinases, proteins from the ubiquitin pathway), neurological development and neurodegenerative diseases and immune recognition. Despite the challenging nature of the analysis of such targets, approximately 170 structures have been determined to date. Here, the impact of high-throughput technologies, such as parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens or the use of mass spectrometry to assist sample preparation, on the structural biology of mammalian protein targets is illustrated through selected examples.
authors.affiliation: CIRMMP, CERM, Via Sacconi 6, Sesto Fiorentino, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Banci
authors.forename: I
authors.initials: I
authors.lastname: Bertini
authors.forename: S
authors.initials: S
authors.lastname: Cusack
authors.forename: R N
authors.initials: RN
authors.lastname: de Jong
authors.forename: U
authors.initials: U
authors.lastname: Heinemann
authors.forename: E Y
authors.initials: EY
authors.lastname: Jones
authors.forename: F
authors.initials: F
authors.lastname: Kozielski
authors.forename: K
authors.initials: K
authors.lastname: Maskos
authors.forename: A
authors.initials: A
authors.lastname: Messerschmidt
authors.forename: R
authors.initials: R
authors.lastname: Owens
authors.forename: A
authors.initials: A
authors.lastname: Perrakis
authors.forename: A
authors.initials: A
authors.lastname: Poterszman
authors.forename: G
authors.initials: G
authors.lastname: Schneider
authors.forename: C
authors.initials: C
authors.lastname: Siebold
authors.forename: I
authors.initials: I
authors.lastname: Silman
authors.forename: T
authors.initials: T
authors.lastname: Sixma
authors.forename: G
authors.initials: G
authors.lastname: Stewart-Jones
authors.forename: J L
authors.initials: JL
authors.lastname: Sussman
authors.forename: J C
authors.initials: JC
authors.lastname: Thierry
authors.forename: Dino
authors.initials: D
authors.lastname: Moras
mesh_terms: D000818:Animals; D005057:Eukaryotic Cells; D015870:Gene Expression; D036281:Genetic Research; D006801:Humans; D007107:Immune System / Q000502:physiology; D013058:Mass Spectrometry; D009369:Neoplasms / Q000235:genetics; D009422:Nervous System Diseases / Q000235:genetics; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000639:trends*
chemical_list: D011506:Proteins