title: DNA sequence-based detection of group B Streptococcus directly from heart valve tissue in a patient with culture-negative endocarditis.
abstract: A human case of culture-negative bacterial endocarditis is presented where conventional methods failed to determine a microbial aetiology. DNA sequencing performed directly on autopsy heart valve tissue revealed Streptococcus agalactiae (group B streptococcus). To our knowledge, this is the first report of DNA sequence-based detection of this organism directly from a heart valve.
authors.affiliation: Department of Pathology and Laboratory Medicine, Calgary, Alberta, Canada.
authors.forename: Kelly
authors.initials: K
authors.lastname: Guggisberg
authors.forename: Robin
authors.initials: R
authors.lastname: Parker
authors.forename: David
authors.initials: D
authors.lastname: Megran
authors.forename: Kunyan
authors.initials: K
authors.lastname: Zhang
authors.forename: Wanda
authors.initials: W
authors.lastname: Lester
authors.forename: Sameer
authors.initials: S
authors.lastname: Elsayed
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004697:Endocarditis, Bacterial / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D017809:Fatal Outcome; D006801:Humans; D008297:Male; D008875:Middle Aged; D013290:Streptococcal Infections / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D013292:Streptococcus agalactiae / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial