Effect of soluble fiber or fructooligosaccharide supplementation upon trinitrobenzenesulphonic acid induced colitis in rats.
OBJECTIVE
To evaluate the effect of soluble fiber or fructooligosaccharide (FOS) supplementation upon trinitrobenzene sulphonic acid (TNBS)-induced colitis in rats.


METHODS
64 Wistar rats were given water, soluble fiber or FOS intragastrically during 14 days prior to colitis induction with TNBS (n=48) or rectal enema with water (n=16; control group). On the 7th or 14th day following colitis induction the rats were weighed and euthanized in order to determine the colon weight/length ratio and macroscopic and microscopic scores.


RESULTS
On the 7th day following colitis induction the body weight had decreased significantly, the colon weight/length ratio had increased and macroscopic and microscopic colon lesions were observed. On the 14th day following colitis induction no difference in body weight was observed, in spite of the persistence of macroscopic and microscopic lesions and increased colon weight/length ratio. Supplementation with soluble fiber or FOS did not revert colon lesions or any of the study parameters. Supplementation with FOS, but not with fiber, was associated with increased colon weight/length ratio on the 14th day.


CONCLUSIONS
Supplementation with soluble fiber or FOS produced no significant impact on TNBS-induced colitis in rats.
Murad-Regadas
Sthela Maria
SM
Department of Surgery, Faculty of Medicine, Federal University of Ceará (UFC), Tocantins, Brazil. smregadas@hospitalsaocarlos.com.br
Souza
Marcellus Henrique Loiola Ponte de
MH
Brito
Gerly Anne de Castro
GA
Rodrigues
Lusmar Veras
LV
Regadas
Francisco Sérgio Pinheiro
FS
Vasconcelos
Paulo Roberto Leitão de
PR
D000284:Administration, Oral; D000285:Administration, Rectal; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D003106:Colon / Q000187:drug effects / Q000473:pathology; D004043:Dietary Fiber / Q000627:therapeutic use*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D008297:Male; D009844:Oligosaccharides / Q000627:therapeutic use*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D013997:Time Factors; D014302:Trinitrobenzenesulfonic Acid
D004043:Dietary Fiber; D009844:Oligosaccharides; C116580:fructooligosaccharide; D014302:Trinitrobenzenesulfonic Acid