[Experimental study on treatment of allergic asthma guinea pigs with different fractions of Modified Zhisousan].
OBJECTIVE
To study the effects of different fractions of Modified Zhisousan (MZ, MZC, MZS) on the contents of nitric oxide (NO), endothelin-1 (ET-1) and eosinophilia (EOS) in the allergic asthma guinea pigs and observe the pathology changes of lung tissue.


METHODS
The number of EOS in the blood and bronchialveolar lavage fluid (BALF) was counted by Wright staining. The contents of ET-1 and No in serum and BALF were analyzed by RIA and nitric acid reductase method. The guinea pig lungs were observed under the optical and electron microscope.


RESULTS
The number of EOS and the contents of ET-1 and NO in model group were higher than those in control group (P < 0.01). The pathological changes of lung were obvious. The number of EOS and the contents of ET-1 and NO were descended remarkably (P < 0.05 or P < 0.01) and the pathological changes in the lung tissue were lightened obviously in MZ and MZC groups, but MZS group had no such effects.


CONCLUSIONS
MZC is the effective part of MZ and the anti-asthmatic mechanisms ware related to its significant reduction in contents of ET-1, NO, EOS and the damage of lung tissue possibly.
Xu
Nai-yu
NY
Department of Pharmacy , Suzhou University, Suzhou 215007, China. Xunaiyu@suda.edu.cn
Gu
Zhen-lun
ZL
Xie
Mei-lin
ML
Zhou
Wen-xuan
WX
Guo
Ci-yi
CY
D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D001249:Asthma / Q000188:drug therapy / Q000473:pathology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D019332:Endothelin-1 / Q000097:blood; D004804:Eosinophils / Q000737:chemistry; D006168:Guinea Pigs; D007958:Leukocyte Count; D008168:Lung / Q000473:pathology*; D051379:Mice; D009569:Nitric Oxide / Q000097:blood; D011208:Powders
D018927:Anti-Asthmatic Agents; D004365:Drugs, Chinese Herbal; D019332:Endothelin-1; D011208:Powders; D009569:Nitric Oxide