title: Rabbit embryonic stem cell lines derived from fertilized, parthenogenetic or somatic cell nuclear transfer embryos.
abstract: Embryonic stem cells were isolated from rabbit blastocysts derived from fertilization (conventional rbES cells), parthenogenesis (pES cells) and nuclear transfer (ntES cells), and propagated in a serum-free culture system. Rabbit ES (rbES) cells proliferated for a prolonged time in an undifferentiated state and maintained a normal karyotype. These cells grew in a monolayer with a high nuclear/cytoplasm ratio and contained a high level of alkaline phosphate activity. In addition, rbES cells expressed the pluripotent marker Oct-4, as well as EBAF2, FGF4, TDGF1, but not antigens recognized by antibodies against SSEA-1, SSEA-3, SSEA-4, TRA-1-10 and TRA-1-81. All 3 types of ES cells formed embryoid bodies and generated teratoma that contained tissue types of all three germ layers. rbES cells exhibited a high cloning efficiency, were genetically modified readily and were used as nuclear donors to generate a viable rabbit through somatic cell nuclear transfer. In combination with genetic engineering, the ES cell technology should facilitate the creation of new rabbit lines.
authors.affiliation: Center for Developmental Biology, Xinhua Hospital, Shanghai Jiao Tong University, School of Medicine, 1665 Kong Jiang Road, Shanghai 200092, P.R. China.
authors.forename: Zhen F
authors.initials: ZF
authors.lastname: Fang
authors.forename: Hui
authors.initials: H
authors.lastname: Gai
authors.forename: You Z
authors.initials: YZ
authors.lastname: Huang
authors.forename: Shan G
authors.initials: SG
authors.lastname: Li
authors.forename: Xue J
authors.initials: XJ
authors.lastname: Chen
authors.forename: Jian J
authors.initials: JJ
authors.lastname: Shi
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Ailian
authors.initials: A
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.forename: Hui Z
authors.initials: HZ
authors.lastname: Sheng
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000502:physiology; D002454:Cell Differentiation; D002460:Cell Line; D019976:Cloning, Organism / Q000379:methods*; D016895:Culture Media, Serum-Free; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D005306:Fertilization; D006801:Humans; D007621:Karyotyping; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D018895:Microsatellite Repeats; D053652:Nuclear Transfer Techniques; D010312:Parthenogenesis; D011817:Rabbits; D013724:Teratoma / Q000378:metabolism; D000097573:Red Fluorescent Protein
chemical_list: D016895:Culture Media, Serum-Free; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein