Dual colour, microarray-based, analysis of 10,000 protease substrates.
A 10,000 member PNA-encoded library of FRET based peptides was synthesised for global analysis of protease cleavage specificity; analysis was achieved using a DNA microarray and consumed minimal quantities of enzyme (60 pmole) and library (3.5 nmole).
Díaz-Mochón
Juan J
JJ
University of Edinburgh, School of Chemistry, Joseph Black Buildings, Midlothian, UKEH9 3JJ.
Bialy
Laurent
L
Bradley
Mark
M
D000595:Amino Acid Sequence; D002917:Chymopapain / Q000378:metabolism*; D003116:Color; D031541:Fluorescence Resonance Energy Transfer; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010447:Peptide Hydrolases / Q000378:metabolism*; D020135:Peptide Nucleic Acids / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D013379:Substrate Specificity
D020135:Peptide Nucleic Acids; D010447:Peptide Hydrolases; D002917:Chymopapain