Genomic relations among 31 species of Mammillaria haworth (Cactaceae) using random amplified polymorphic DNA.
Thirty-one species of Mammillaria were selected to study the molecular phylogeny using random amplified polymorphic DNA (RAPD) markers. High amount of mucilage (gelling polysaccharides) present in Mammillaria was a major obstacle in isolating good quality genomic DNA. The CTAB (cetyl trimethyl ammonium bromide) method was modified to obtain good quality genomic DNA. Twenty-two random decamer primers resulted in 621 bands, all of which were polymorphic. The similarity matrix value varied from 0.109 to 0.622 indicating wide variability among the studied species. The dendrogram obtained from the unweighted pair group method using arithmetic averages (UPGMA) analysis revealed that some of the species did not follow the conventional classification. The present work shows the usefulness of RAPD markers for genetic characterization to establish phylogenetic relations among Mammillaria species.
Mattagajasingh
Ilwola
I
DNA Fingerprinting Laboratory, Division of Plant Biotechnology, Regional Plant Resource Centre (R.P.R.C), IRC Village, Bhubaneswar 751 015, Orissa, India.
Mukherjee
Arup Kumar
AK
Das
Premananda
P
D029421:Cactaceae / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D018745:Genome, Plant; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
D017931:DNA Primers; D018744:DNA, Plant