Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b.
Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump.
Kumar
Ayush
A
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA.
Chua
Kim-Lee
KL
Schweizer
Herbert P
HP
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016957:Burkholderia pseudomallei / Q000187:drug effects* / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002701:Chloramphenicol / Q000494:pharmacology; D004251:DNA Transposable Elements; D024901:Drug Resistance, Multiple, Bacterial; D015964:Gene Expression Regulation, Bacterial; D005821:Genetic Techniques; D005822:Genetic Vectors; D016680:Genome, Bacterial; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic; D014295:Trimethoprim / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D002701:Chloramphenicol; D014295:Trimethoprim