title: Co-culture of bone marrow fibroblasts and endothelial cells on modified polycaprolactone substrates for enhanced potentials in bone tissue engineering.
abstract: The creation of a vascularized bed makes the survival of seeded cells on 3-dimensional scaffolds much more likely. However, relying purely on random capillary ingrowth into the porous scaffolds from the host may compromise vascularization of a scaffold. One solution is to transplant cells capable of differentiating into new blood vessels into the scaffolds to accelerate the creation of a vascularized scaffold. Because endothelial cells are the key cells involved in blood vessel formation, the present study was designed to investigate the development of a biomaterial surface that supports endothelial cell attachment and proliferation. The subsequent effects of the material surface modifications on the differentiation and proliferation of human bone marrow-derived fibroblasts (HBMFs) when grown in co-culture with a human bone marrow endothelial cell line (HBMEC-60) were studied. Endothelialization studies showed that the gelatin-coated and hydroxyapatite-coated substrates were superior for HBMEC-60 attachment and proliferation to hydrolyzed-only or untreated polycaprolactone substrates. Co-culture studies showed that the presence of the HBMEC-60 specifically enhanced HBMF cell proliferation and differentiation and that this effect was not observed with co-culture with skin fibroblasts. It is concluded that the co-culture of endothelial cells with HBMFs could be a promising culture system for bone tissue- engineering applications.
authors.affiliation: Botnar Research Centre, Institute of Musculoskeletal Sciences, University of Oxford, United Kingdom. cleo.choong@gmail.com
authors.forename: Cleo S N
authors.initials: CS
authors.lastname: Choong
authors.forename: Dietmar W
authors.initials: DW
authors.lastname: Hutmacher
authors.forename: James T
authors.initials: JT
authors.lastname: Triffitt
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D018786:Bone Substitutes; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D020099:Coated Materials, Biocompatible; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D006801:Humans; D018919:Neovascularization, Physiologic; D009928:Organ Specificity; D011091:Polyesters; D012867:Skin / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods
chemical_list: D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone