title: [Protective effect of reduced glutathione on cytotoxicity induced by hexavalent chromium [Cr(VI)] in L-02 hepatocyte].
abstract: OBJECTIVE
  To explore the effect of reduced glutathione (GSH) on cytotoxicity induced by hexavalent chromium (Cr(VI)) in L-02 hepatocyte.
  
  
  METHODS
  The test has three groups:the groups of (Cr(VI), the groups of GSH, the groups of Cr (VI) and GSH. The survival rate of L-02 hepatocyte is assessed on the reductions of tetrazolium dye (MTT).
  
  
  RESULTS
  Significant cytotoxicities of L-02 hepatocyte were observed at the concenations of 2,4,8,16,32 and 64 micromol/L Cr (VI). Concentration-dependent decrease in cell survival rate of Cr (VI)-treated L-02 hepatocytes were observed (r = -0.910) Protective effect on all concentrations of Cr(VI) (2 - 64 micromol/L) at the dose of 20 micromol/LGSH were found.
  
  
  CONCLUSIONS
  The results demonstrated that proper concentrations of GSH could have protective effect on cytoxicity induced by Cr(VI) in L-02 hepatocyte. GSH of too low or too high concentrations don't has this effect.
authors.affiliation: Institute for Occupational Health and Poison Control, China CDC, Beijing 100050, China.
authors.forename: Hai-hua
authors.initials: HH
authors.lastname: Wang
authors.forename: Jing-wei
authors.initials: JW
authors.lastname: Xiao
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Cai-gao
authors.initials: CG
authors.lastname: Zhong
mesh_terms: D002460:Cell Line; D002857:Chromium / Q000633:toxicity*; D005978:Glutathione / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D020011:Protective Agents / Q000494:pharmacology*
chemical_list: D020011:Protective Agents; D002857:Chromium; C074702:chromium hexavalent ion; D005978:Glutathione