title: Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells.
abstract: Erythroblast enucleation is thought to be largely dependent on signals mediated by other cells, such as macrophages. In an attempt to improve the in vitro production of red blood cells (RBCs) from immature hematopoietic progenitor cells, we have developed a method to produce enucleated RBCs efficiently in the absence of feeder cells. Our method may represent an efficient way to produce transfusable RBCs on a large scale from hematopoietic progenitors.
authors.affiliation: Cell Engineering Division, RIKEN BioResource Center, Koyadai 3-1-1, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Miharada
authors.forename: Takashi
authors.initials: T
authors.lastname: Hiroyama
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sudo
authors.forename: Toshiro
authors.initials: T
authors.lastname: Nagasawa
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002467:Cell Nucleus; D004900:Erythroblasts / Q000166:cytology* / Q000502:physiology; D006021:Glycophorins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D011990:Receptors, Transferrin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; C068126:CD71 antigen; D006021:Glycophorins; D011990:Receptors, Transferrin