title: S100P is an early developmental marker of pancreatic carcinogenesis.
abstract: OBJECTIVE
  Our goal was to clarify the involvement and clinical significance of S100P in pancreatic carcinogenesis.
  
  
  METHODS
  We examined S100P expression in 45 bulk pancreatic tissues; in microdissected cells, including invasive ductal carcinoma (IDC) cells (20 sections), pancreatic intraepithelial neoplasia (PanIN) cells (12 sections), intraductal papillary mucinous neoplasm (IPMN) cells (19 sections), and normal epithelial cells (11 sections); and in pancreatic juice samples from 99 patients with pancreatic diseases (32 cancer, 35 IPMN, and 32 chronic pancreatitis samples). We used quantitative real-time reverse transcription-PCR with gene-specific priming to measure S100P in these various types of samples.
  
  
  RESULTS
  In bulk tissue analyses, pancreatic cancer and IPMN expressed significantly higher levels of S100P than did nonneoplastic pancreas (P<0.017 and P=0.0013, respectively). Microdissection analyses revealed that IPMN expressed significantly higher levels of S100P than did IDC (P<0.0001) and PanIN (P=0.0031), although S100P expression did not differ between IDC and PanIN (P=0.077). In pancreatic juice analyses, cancer and IPMN juice expressed significantly higher levels of S100P than did pancreatitis juice (both P<0.0001). Receiver operating characteristic curve analyses revealed that measurement of S100P in pancreatic juice was useful for discriminating neoplastic disease from chronic pancreatitis (area under the curve=0.837; 95% confidence interval, 0.749-0.903).
  
  
  CONCLUSIONS
  S100P may be an early developmental marker of pancreatic carcinogenesis, and measurement of S100P in pancreatic juice may be useful for early detection of pancreatic cancer or screening of early pancreatic carcinogenesis.
authors.affiliation: Department of Surgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Kenoki
authors.initials: K
authors.lastname: Ohuchida
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Mizumoto
authors.forename: Takuya
authors.initials: T
authors.lastname: Egami
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Kei
authors.initials: K
authors.lastname: Fujii
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Konomi
authors.forename: Eishi
authors.initials: E
authors.lastname: Nagai
authors.forename: Koji
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Masazumi
authors.initials: M
authors.lastname: Tsuneyoshi
authors.forename: Masao
authors.initials: M
authors.lastname: Tanaka
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism*; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism; D002291:Carcinoma, Papillary / Q000378:metabolism; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D018450:Disease Progression; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism; D010189:Pancreatic Juice / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D015415:Biomarkers; D002135:Calcium-Binding Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C103003:S100P protein, human