title: [Detection and typing of dengue virus using polymerase chain reaction and microwell plate hybridization].
abstract: OBJECTIVE
  To establish a specific, sensitive and practicable method for detection and typing of dengue virus.
  
  
  METHODS
  Based on the genomic sequence analysis of dengue virus types 1-4, 4 pairs of primers were designed. The specific capture probes of dengue virus types 1-4 were amplified using RT-PCR, cloned and sequenced before using them for precoating the microwell plate. The samples were amplified using biotin-labeled forward primer and reverse primer, and microwell plate hybridization was carried out for detection and typing of dengue virus types 1-4.
  
  
  RESULTS
  The absorbance of the positive samples were higher than 0.5, while the average absorbance of the negative samples was lower than 0.1, with the S/N higher than 10.
  
  
  CONCLUSIONS
  The method of PCR-ELISA we established for early detection and typing of all 4 dengue viruses seretypes.
authors.affiliation: Disease Control and Prevention Center of Guangzhou Command, Guangzhou 510507, China. renruiwen@hotmail.com
authors.forename: Rui-wen
authors.initials: RW
authors.lastname: Ren
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Xu
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Li
authors.forename: Mei-yu
authors.initials: MY
authors.lastname: Fang
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Liu
authors.forename: An-de
authors.initials: AD
authors.lastname: Ma
mesh_terms: D003716:Dengue Virus / Q000145:classification / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012703:Serotyping / Q000379:methods