Morphological analysis of three wound-cleaning processes on potentially contamined wounds in rats.
OBJECTIVE
To evaluate the inflammatory response of potentially infected wounds treated with isotonic saline solution, chlorhexidine and PVP-I, seven days after surgery.


METHODS
Thirty-two male rats were used, divided into 4 groups. All animals had their surgical wounds infected with a standard bacterial inoculum. Control group (A): animals had their surgical wounds sutured without any kind of cleaning. Saline solution group (B): animals had their wounds cleaned with saline solution. Chlorhexidine group (C): animals had their wounds cleaned with chlorhexidine. PVP-I group (D): animals had their wounds cleaned with PVP-I. Seven days after surgery, all the animals had their skin submitted to microscopic and macroscopic evaluation.


RESULTS
Edema was found on all histological slices analyzed, as well as vascular proliferation and congestion. Groups A and D showed presence of mild neutrophilic infiltrate, and moderate lymphocytic and macrophage infiltrate. Group B showed severe neutrophilic, macrophage, and lymphocytic infiltrate. Group C showed moderate neutrophilic, macrophage, and lymphocytic infiltrate.


CONCLUSIONS
Group D was the group which showed inflammatory infiltrate most similar to the group that was not submitted to treatment.
d'Acampora
Armando José
AJ
Surgical Technique and Experimental Surgery, Department of Surgery, University of Santa Catarina (UFSC), SC, Brazil. dacampora@gmail.com
Vieira
Daniella Serafin Couto
DS
Silva
Marcos Túlio
MT
Farias
Débora Cadore de
DC
Tramonte
Ricardo
R
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D002710:Chlorhexidine / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005355:Fibrosis / Q000473:pathology; D006097:Granulation Tissue / Q000473:pathology; D007552:Isotonic Solutions / Q000627:therapeutic use; D008297:Male; D011206:Povidone-Iodine / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000627:therapeutic use*; D013530:Surgical Wound Infection / Q000188:drug therapy* / Q000473:pathology; D016896:Treatment Outcome
D000891:Anti-Infective Agents, Local; D007552:Isotonic Solutions; D012965:Sodium Chloride; D011206:Povidone-Iodine; D002710:Chlorhexidine