Alginate/carbon composite beads for laccase and glucose oxidase encapsulation: application in biofuel cell technology.
Alginate-carbon beads were prepared in order to develop a biocompatible matrix for laccase and glucose oxidase immobilization for application in biofuel cell technology. The enzyme loading capacity was high (91%) in pure alginate beads for glucose oxidase. For laccase, the loading capacity was enhanced from 75% to 83% by introducing carbon. Desorption out of the matrix was controlled by the enzymes' diffusion and reached a plateau after 40 h for laccase and 70 h for glucose oxidase. Two-thirds of both enzymes was irreversibly retained inside the alginate beads. This proportion increased to 80% for laccase in combined alginate/carbon beads. Half-life of the adsorbed enzyme was enhanced to 74 days for laccase in carbon/alginate beads and 45 days for glucose oxidase in pure alginate as compared to 38 days and 23 days for free enzymes, respectively.
Khani
Zoreh
Z
Institut Européen des Membranes, UMR 5635, Place Eugène Bataillon, CC 047, 34293, Montpellier Cedex 5, France.
Jolivalt
Claude
C
Cretin
Marc
M
Tingry
Sophie
S
Innocent
Christophe
C
D000327:Adsorption; D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D002384:Catalysis; D004058:Diffusion; D004563:Electrochemistry / Q000379:methods; D004798:Enzymes / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D007700:Kinetics; D042845:Laccase / Q000737:chemistry*; D013997:Time Factors
D000464:Alginates; D001672:Biocompatible Materials; D004798:Enzymes; D002244:Carbon; D005949:Glucose Oxidase; D042845:Laccase