title: Induced trefoil factor family 1 expression by trans-differentiating Clara cells in a murine asthma model.
abstract: Asthma is a chronic inflammatory disease of the airways that is accompanied by goblet cell metaplasia and mucus hypersecretion. Trefoil factor family (TFF) peptides represent major secretory products of the respiratory tract and are synthesized together with mucins. In the murine lung, TFF2 is mainly expressed, whereas TFF1 transcripts represent only a minor species. TFF peptides are well known for their motogenic and anti-apoptotic effects, and they modulate the inflammatory response of bronchial epithelial cells. Here, an established mouse model of asthma was investigated (i.e., exposure to Aspergillus fumigatus [AF] antigens). RT-PCR analysis of lung tissue showed elevated levels particularly of TFF1 transcripts in AF-sensitized/challenged animals. In contrast, transcripts encoding Clara cell secretory protein (CCSP/CC10) were strongly diminished in these animals. For comparison, the expression of the goblet cell secretory granule marker mCLCA3/Gob-5, the mucins Muc1-Muc6 and Muc19, and the secretoglobins ScgB3A1 and ScgB3A2, as well as the mammalian ependymin-related gene MERP2, were monitored. Immunohistochemistry localized TFF1 mainly in cells with a mixed phenotype (e.g., TFF1-positive cells stain with the lectin wheat germ agglutinin (WGA), which recognizes mucins characteristic of goblet cells). In addition, these cells express CCSP/CC10, a Clara cell marker. When compared with mucins or CCSP/CC10, TFF1 was stored in a different population of secretory granules localized at the more basolateral portion of these cells. Thus, the results presented indicate for the first time that allergen exposure leads to the trans-differentiation of Clara cells toward a TFF1-expressing mucous phenotype.
authors.affiliation: Institut für Molekularbiologie und Medizinische Chemie, Otto-von-Guericke-Universität, Magdeburg; and Fraunhofer-Institut für Toxikologie und Experimentelle Medizin, Hannover, Germany.
authors.forename: Irina
authors.initials: I
authors.lastname: Kouznetsova
authors.forename: Caroline E
authors.initials: CE
authors.lastname: Chwieralski
authors.forename: Ralf
authors.initials: R
authors.lastname: Bälder
authors.forename: Margitta
authors.initials: M
authors.lastname: Hinz
authors.forename: Armin
authors.initials: A
authors.lastname: Braun
authors.forename: Norbert
authors.initials: N
authors.lastname: Krug
authors.forename: Werner
authors.initials: W
authors.lastname: Hoffmann
mesh_terms: D000818:Animals; D001232:Aspergillus fumigatus; D001249:Asthma / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D001992:Bronchoalveolar Lavage Fluid; D002454:Cell Differentiation; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation; D008168:Lung / Q000166:cytology* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009077:Mucins / Q000378:metabolism; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071163:Trefoil Factor-1; D014598:Uteroglobin / Q000378:metabolism
chemical_list: D016207:Cytokines; D009077:Mucins; D010455:Peptides; D012333:RNA, Messenger; C472724:Tff1 protein, mouse; D000071163:Trefoil Factor-1; D007073:Immunoglobulin E; D014598:Uteroglobin