title: [Production and characterization of gold labeled monoclonal antibody against MC-LR].
abstract: OBJECTIVE
  Production of the gold labeled monoclonal antibody against MC-LR. Study the simple mechanism of the conjugation by the spectral analysis.
  
  
  METHODS
  Production and purification monoclonal antibodies against MC-LR by hybridoma technology. Production the 20nm colloidal gold and label the monoclonal antibody. Study the gold--McAb conjugate by the spectroanalysis.
  
  
  RESULTS
  Monoclonal antibody produced by the hybridoma cells were tested for subtypes and designated as IgG2a for 8C11. The titers of antibody in ascites was 108.The IC50 for MC-LR was around 3ng/ml. The IgG molecule weight was 1.5 x 10(5). The structure of the antibody was altered to more tighter and stabler.
  
  
  CONCLUSIONS
  Product the gold labeled monoclonalantibody against MC-LR conjugate successfully and keep the antige-binding determinant.
authors.affiliation: Southern Yangtz University, Wuxi 214036, China.
authors.forename: Xiao-lian
authors.initials: XL
authors.lastname: Zhao
authors.forename: Wei-rong
authors.initials: WR
authors.lastname: Sun
authors.forename: Xiu-lan
authors.initials: XL
authors.lastname: Sun
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D017979:Gold Colloid; D006825:Hybridomas; D007118:Immunoassay / Q000379:methods; D008387:Marine Toxins; D051379:Mice; D008807:Mice, Inbred BALB C; D052998:Microcystins / Q000032:analysis* / Q000276:immunology*; D011934:Reagent Strips
chemical_list: D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D008387:Marine Toxins; D052998:Microcystins; D011934:Reagent Strips; C057862:cyanoginosin LR