title: Antioxidant redox sensors based on DNA modified carbon screen-printed electrodes.
abstract: Antioxidant redox sensors based on DNA modified carbon screen-printed electrodes were developed. The carbon ink was doped with TiO2 nanoparticles, onto which double-strand DNA was adsorbed. A redox mediator, namely, tris-2,2'-bipyridine ruthenium(II) [Ru(bpy)3(2+)] was electrooxidized on the electrode surface to subsequently oxidize both the adsorbed ds-DNA and the antioxidants in solution. The resulting oxidation damage of the adsorbed ds-DNA was then detected by square wave voltammetry in a second solution containing only Ru(bpy)3Cl2 at a low concentration (microM). A kinetic model was developed to study the protecting role of antioxidants in aqueous solutions. The electrochemical sensor has been applied to evaluate the redox antioxidant capacity of different molecules.
authors.affiliation: Laboratoire d'Electrochimie Physique et Analytique, Station 6, Ecole Polytechnique Fédérale de Lausanne, CH-1015, Lausanne, Switzerland.
authors.forename: Jifeng
authors.initials: J
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Su
authors.forename: Grégoire
authors.initials: G
authors.lastname: Lagger
authors.forename: Philippe
authors.initials: P
authors.lastname: Tacchini
authors.forename: Hubert H
authors.initials: HH
authors.lastname: Girault
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004566:Electrodes; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D014025:Titanium / Q000737:chemistry
chemical_list: D000975:Antioxidants; C009495:titanium dioxide; D002244:Carbon; D004247:DNA; D014025:Titanium