Electroenzymatic polypyrrole-intercalator sensor for the determination of West Nile virus cDNA.
The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.
Cosnier
Serge
S
Ionescu
Rodica E
RE
Herrmann
Sebastien
S
Bouffier
Laurent
L
Demeunynck
Martine
M
Marks
Robert S
RS
D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D014902:West Nile virus / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole