title: Composition of the volatile fraction of Ocotea bofo Kunth (Lauraceae) calyces by GC-MS and NMR fingerprinting and its antimicrobial and antioxidant activity.
abstract: The chemical composition of the essential oil obtained by steam distillation of the floral calyces of Ocotea bofo Kunth (Lauraceae) was studied by means of GC, GC-MS, and 1H, 13C, and bidimensional NMR (COSY, HSQC, HMBC). Twenty-five constituents were identified, and estragole (48.7%), alpha-phellandrene (19.6%) and sabinene (10.4%) were found to be the major components. Antimicrobial activity against six aerobic bacteria and five yeasts and antioxidant activity performed by photochemiluminescence (PCL), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and beta-carotene bleaching assays are reported. The oil showed fair inhibiting properties against bacteria and a good inhibition against most yeasts. Its radical scavenging and chain-breaking antioxidant properties were comparable to or better than those provided by synthetic controls. Particular emphasis has been given to the use of NMR as a fast and reliable tool to discriminate O. bofo essential oil from other commercial anethole- and estragole-rich oils, namely, Illicium verum, Foeniculum vulgare, and Artemisia dracunculus.
authors.affiliation: Dipartimento delle Risorse Naturali e Culturali, Università degli Studi di Ferrara, Corso Ercole d'Este 32, 44100 Ferrara, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Guerrini
authors.forename: Gianni
authors.initials: G
authors.lastname: Sacchetti
authors.forename: Mariavittoria
authors.initials: M
authors.lastname: Muzzoli
authors.forename: Gabriela
authors.initials: G
authors.lastname: Moreno Rueda
authors.forename: Alessandro
authors.initials: A
authors.lastname: Medici
authors.forename: Elena
authors.initials: E
authors.lastname: Besco
authors.forename: Renato
authors.initials: R
authors.lastname: Bruni
mesh_terms: D000081248:Allylbenzene Derivatives; D000840:Anisoles / Q000032:analysis / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D035264:Flowers / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D027444:Ocotea / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology; D010851:Picrates; D014835:Volatilization; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000081248:Allylbenzene Derivatives; D000840:Anisoles; D000890:Anti-Infective Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D009822:Oils, Volatile; D010851:Picrates; D019207:beta Carotene; C007633:estragole; C004931:1,1-diphenyl-2-picrylhydrazyl