Localization and diurnal variations of carbonic anhydrase mRNA expression in the inner ear of the rainbow trout Oncorhynchus mykiss.
Physiological studies have suggested that carbonic anhydrase (CA) plays a central role in otolith biomineralization via ion transport. However, the presence and exact function of CA in the inner ear have not been determined. In the present study, to investigate the localization of CA and its involvement in otolith calcification, we cloned two cDNAs encoding CAs from the rainbow trout sacculus. These two cDNAs, designated rainbow trout CAa (rtCAa) and rtCAb, both had an open reading frame encoding 260 amino acids with a sequence identity of 78%. Remarkably, rtCAb has a high degree of homology (82%) with "high activity CA" in the zebrafish, and its mRNA expression showed variation in the range 1.9-11.4 x 10(4) copies/ng total RNA in the sacculus. In contrast, rtCAa mRNA was constantly expressed at approximately 3 x 10(4) copies/ng total RNA. In situ hybridization revealed that rtCAb mRNA was strongly expressed in the distal squamous epithelial cells and transitional epithelial cells, except the mitochondria-rich cells, whereas, rtCAa was localized in extrasaccular tissue. These results suggest that the rtCAb isozyme is involved in the daily increment formation and calcification of otoliths via phase and spatial differences of the bicarbonate supply to the endolymph.
Tohse
Hidekazu
H
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan. tohse@fish.hokudai.ac.jp
Murayama
Emi
E
Ohira
Tsuyoshi
T
Takagi
Yasuaki
Y
Nagasawa
Hiromichi
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002256:Carbonic Anhydrases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D002940:Circadian Rhythm; D018076:DNA, Complementary; D007758:Ear, Inner / Q000033:anatomy & histology / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism; D010037:Otolithic Membrane / Q000201:enzymology*
D018076:DNA, Complementary; D007527:Isoenzymes; D002256:Carbonic Anhydrases