Design, construction and performance of the most efficient biomass producing E. coli bacterium.
Inverse metabolic engineering based on elementary mode analysis was applied to maximize the biomass yield of Escherchia coli MG1655. Elementary mode analysis was previously employed to identify among 1691 possible pathways for cell growth the most efficient pathway with maximum biomass yield. The metabolic network analysis predicted that deletion of only 6 genes reduces the number of possible elementary modes to the most efficient pathway. We have constructed a strain containing these gene deletions and we evaluated its properties in batch and in chemostat growth experiments. The results show that the theoretical predictions are closely matched by the properties of the designed strain.
Trinh
Cong T
CT
Department of Chemical Engineering and Materials Science, BioTechnology Institute, University of Minnesota, St. Paul, MN 55108, USA.
Carlson
Ross
R
Wlaschin
Aaron
A
Srienc
Friedrich
F
D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D017353:Gene Deletion; D005818:Genetic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D016133:Polymerase Chain Reaction
D017931:DNA Primers