[Excitotoxic effects of glutamate on the in vitro spiral ganglion cells].
OBJECTIVE
To explore the excitotoxic effects of glutamate on the in vitro primarily cultured spiral ganglion cell (SGC) of rat.


METHODS
Spiral ganglion cells (SGCs) were cultured in vitro for 4 days, and exposed to 1 mmol/L glutamate for 24 hours. Damaged cells double-labeling with Hoechest33258 and PI were observed by fluorescence microscope. Ffluo-3 and CLSM for measurement of intracellular calcium levels were also utilized.


RESULTS
Most cells were damaged and the intracellular calcium increased and after exposure to 1 mmol/L glutamate (P < 0.05).


CONCLUSIONS
Glutamate excitotoxicity was associated with free intracellular calcium ion concentration elevation in SGC.
Yan
Kaisheng
K
Department of Otolaryngology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.
Xue
Qiuhong
Q
Luo
Linghui
L
Gong
Shusheng
S
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000032:analysis; D002478:Cells, Cultured; D018698:Glutamic Acid / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013136:Spiral Ganglion / Q000166:cytology / Q000187:drug effects*
D018698:Glutamic Acid; D002118:Calcium