Evaluation of inactivation methods for severe acute respiratory syndrome coronavirus in noncellular blood products.
BACKGROUND
Severe acute respiratory syndrome coronavirus (SARS-CoV) has been detected in the blood of infected individuals, which may have the potential to contaminate donated blood and plasma-derived products in the event of a future outbreak. Effective methods for inactivating the SARS-CoV in protein solutions are described in this report.


METHODS
Heat, ultraviolet (UV) irradiation, octanoic acid, and solvent/detergent (S/D) methods were tested individually for their ability to inactivate SARS-CoV in protein solutions appropriately mimicking blood-derived products. Treated samples were tested for inactivation in a tissue culture growth assay.


RESULTS
Viral inactivation by heat treatment at 60 degrees C required 15 to 30 minutes to inactivate the SARS-CoV. UVC efficiently inactivated SARS-CoV in 40 minutes, whereas UVA required the addition of psoralen to enhance inactivation of the virus. The presence of bovine serum albumin limited the ability of UVC and UVA to inactivate SARS-CoV and octanoic acid treatment does not reduce the infectivity of SARS-CoV-spiked protein solutions. S/D treatment required 2, 4, and up to 24 hours for Triton X-100, Tween 80, and sodium cholate inactivation, respectively.


CONCLUSIONS
Heat, UVC irradiation, and S/D treatments effectively inactivate SARS-CoV, whereas octanoic acid treatment is insufficient for inactivation of the virus.
Darnell
Miriam E R
ME
Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Bethesda, Maryland, USA.
Taylor
Deborah R
DR
D000818:Animals; D001782:Blood Donors; D002210:Caprylates / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D006358:Hot Temperature; D006801:Humans; D010949:Plasma / Q000821:virology*; D045473:Severe acute respiratory syndrome-related coronavirus; D045169:Severe Acute Respiratory Syndrome / Q000517:prevention & control; D013997:Time Factors; D014466:Ultraviolet Rays; D014709:Vero Cells; D038301:Virus Inactivation / Q000187:drug effects* / Q000528:radiation effects*
D002210:Caprylates; C031492:octanoic acid