Fatty acids by high-performance liquid chromatography and evaporative light-scattering detector.
A high-performance liquid chromatographic (HPLC) separation method with an evaporative light-scattering detector (ELSD) has been developed for the separation and quantitative analysis of fatty acid methyl esters (FAME) in three different oils. Reverse-phased C18 HPLC separation of 13 FAME is achieved using a methanol/water eluent mixture. The retention times (RT) reflect the elution behavior of these compounds on C18 reversed-phase HPLC. The proposed method is tested on: soybean oil (Glycine max L.) as reference sample, rice bran oil (Oryza sativa L.), pumpkin seed oil (Cucurbita pepo L.) and algal oil (Arthrospira platensis Nordst.).
Bravi
Elisabetta
E
Department of Economic and Food Sciences, Section of Food Technology and Biotechnology, University of Perugia, Via S. Costanzo n.c.n., 06126 Perugia, Italy. e.bravi@unipg.it
Perretti
Giuseppe
G
Montanari
Luigi
L
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D028464:Cucurbita / Q000737:chemistry; D056890:Eukaryota / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis* / Q000302:isolation & purification; D008027:Light; D010938:Plant Oils / Q000737:chemistry; D000073879:Rice Bran Oil; D012542:Scattering, Radiation; D013024:Soybean Oil / Q000737:chemistry
D005227:Fatty Acids; D010938:Plant Oils; D013024:Soybean Oil; D000073879:Rice Bran Oil