Extending the scope of NMR spectroscopy with microcoil probes.
Capillary NMR (CapNMR) spectroscopy has emerged as a major breakthrough for increasing the mass-sensitivity of NMR spectroscopic analysis and enabling the combination of NMR spectroscopy with other analytical techniques. Not only is the acquisition of high-sensitivity spectra getting easier but the quality of CapNMR spectra obtained in many small-molecule applications exceeds what can be accomplished with conventional designs. This Minireview discusses current CapNMR technology and its applications for the characterization of mass-limited, small-molecule and protein samples, the rapid screening of small-molecule or protein libraries, as well as hyphenated techniques that combine CapNMR with other analytical methods.
Schroeder
Frank C
FC
Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA. frank_schroeder@hms.harvard.edu
Gronquist
Matthew
M
D001688:Biological Products / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D011506:Proteins / Q000737:chemistry; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013256:Steroids / Q000737:chemistry
D001688:Biological Products; D011506:Proteins; D013256:Steroids