A rapid assay of the sialidase activity in species of the Bacteroides fragilis group by using peanut lectin hemagglutination.
In this study, a novel, simple and rapid hemagglutination assay by using a peanut lectin to detect a neuraminidase activity in strains of the Bacteroides fragilis group was developed. One hundred and fourteen species of the B. fragilis group isolated from children with and without diarrhea and 15 reference strains were evaluated. Neuraminidase production was determined by using the method above described and its inhibition was observed by using galactose. The neuraminidase production was observed in 54 (84.37%) diarrhea and in 43 (86%) non-diarrhea strains. HA titers were ranged from 2 to 32. This neuraminidase assays based on PNA hemagglutination is highly sensitive, reproducible and could be used as a tool to detect the sialidase activity in anaerobic bacteria, particularly, in species of the B. fragilis group.
Nakano
Viviane
V
Laboratório de Anaeróbios, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
Fontes Piazza
Roxane Maria
RM
Avila-Campos
Mario Julio
MJ
D001441:Bacteroides fragilis / Q000145:classification / Q000201:enzymology* / Q000472:pathogenicity; D002648:Child; D003967:Diarrhea / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D009439:Neuraminidase / Q000378:metabolism*; D019887:Peanut Agglutinin / Q000494:pharmacology
D019887:Peanut Agglutinin; D009439:Neuraminidase