[Comparison of different methods for examining the feces of suckling piglets for Isospora suis].
A highly sensitive diagnostic method is of great importance for detection of I. suis (Isospora suis). In the present study KSFV (combined sedimentation-flotation method) using different flotation solutions (NaCl, ZnSO4, NaCl-sucrose) was investigated regarding sensitivity, costs and expenditure of human labour in comparison to FV (flotation method) using NaCl-sucrose solution and to AM (autofluorescence microscopy). Ninety faecal samples were examined. Using AM, oocysts were detected in 83.3% of the samples. In cases of KSFV and FV, respectively, 41.1% to 53.3% of samples were tested positive. The quantity of detected oocysts was markedly higher in AM than in KSFV or FV. Therefore AM is the most sensitive of the comprised methods. The expenditure of labour is lowest in AM, time exposure is lower in FV than in KSFV. For performance of AM a fluorescence microscope is required contrary to KSFV and FV.
Kuhnert
Yvonne
Y
Institut für Parasitologie, Veterinärmedizinische Fakultät, Universität Leipzig. kuhnert@vetmed.uni-leipzig.de
Schmäschke
Ronald
R
Daugschies
Arwid
A
D000818:Animals; D000833:Animals, Suckling; D003937:Diagnosis, Differential; D005243:Feces / Q000469:parasitology*; D007549:Isospora / Q000302:isolation & purification*; D021865:Isosporiasis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D008856:Microscopy, Fluorescence / Q000662:veterinary; D010270:Parasite Egg Count / Q000662:veterinary; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000469:parasitology