title: Toward continuous glucose monitoring with planar modified biosensors and microdialysis. Study of temperature, oxygen dependence and in vivo experiment.
abstract: Glucose biosensors based on the use of planar screen-printed electrodes modified with an electrochemical mediator and with glucose oxidase have been optimised for their application in the continuous glucose monitoring in diabetic patients. A full study of their operative stability and temperature dependence has been accomplished, thus giving useful information for in vivo applications. The effect of dissolved oxygen concentration in the working solution was also studied in order to evaluate its effect on the linearity of the sensors. Glucose monitoring performed with serum samples was performed to evaluate the effect of matrix components on operative stability and demonstrated an efficient behaviour for 72 h of continuous monitoring. Finally, these studies led to a sensor capable of detecting glucose at concentrations as low as 0.04 mM and with a good linearity up to 2.0 mM (at 37 degrees C) with an operative stability of ca. 72 h, thus demonstrating the possible application of these sensors for continuous glucose monitoring in conjunction with a microdialysis probe. Moreover, preliminary in vivo experiments for ca. 20 h have demonstrated the feasibility of this system.
authors.affiliation: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy. francesco.ricci@uniroma2.it
authors.forename: Francesco
authors.initials: F
authors.lastname: Ricci
authors.forename: Felice
authors.initials: F
authors.lastname: Caprio
authors.forename: Alessandro
authors.initials: A
authors.lastname: Poscia
authors.forename: Francesco
authors.initials: F
authors.lastname: Valgimigli
authors.forename: Dimitri
authors.initials: D
authors.lastname: Messeri
authors.forename: Elena
authors.initials: E
authors.lastname: Lepori
authors.forename: Giorgio
authors.initials: G
authors.lastname: Dall'Oglio
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Palleschi
authors.forename: Danila
authors.initials: D
authors.lastname: Moscone
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D015190:Blood Glucose Self-Monitoring; D004285:Dogs; D006801:Humans; D017551:Microdialysis; D010100:Oxygen; D013696:Temperature
chemical_list: D001786:Blood Glucose; D010100:Oxygen