Specific primers for rapid detection of Microsporum audouinii by PCR in clinical samples.
This report describes application of PCR fingerprinting to identify common species of dermatophytes using the microsatellite primers M13, (GACA)4, and (GTG)5. The initial PCR analysis rendered a specific DNA fragment for Microsporum audouinii, which was cloned and sequenced. Based on the sequencing data of this fragment, forward (MA_1F) and reverse (MA_1R) primers were designed and verified by PCR to establish their reliability in the diagnosis of M. audouinii. These primers produced a singular PCR band of 431 bp specific only to strains and isolates of M. audouinii, based on a global test of 182 strains/isolates belonging to 11 species of dermatophytes. These findings indicate these primers are reliable for diagnostic purposes, and we recommend their use in laboratory analysis.
Roque
H D
HD
Lab. Micologia, Instituto de Higiene e Medicina Tropical/CREM, Universidade Nova de Lisboa, Portugal.
Vieira
R
R
Rato
S
S
Luz-Martins
M
M
D000293:Adolescent; D000328:Adult; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D003881:Dermatomycoses / Q000175:diagnosis / Q000382:microbiology*; D005260:Female; D006197:Hair / Q000382:microbiology; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008864:Microsporum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008875:Middle Aged; D009262:Nails / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology; D014006:Tinea Capitis / Q000175:diagnosis / Q000382:microbiology*
D017931:DNA Primers; D004271:DNA, Fungal