Medicago truncatula transformation using leaf explants.
Legumes have long been recalcitrant to efficient Agrobacterium tumefaciens-mediated transformation. The choice and use of model legume plants (Medicago truncatula and Lotus japonicus) for molecular studies has triggered extensive studies devoted to the development of efficient Agrobacterium-mediated transformation protocols for these two plants. In M. truncatula, transformation protocols rely on the use of highly regenerable lines obtained by recurrent in vitro culture selection. These protocols are based on Agrobacterium-mediated transformation of M. truncatula followed by somatic embryogenesis-mediated plant regeneration. We describe here the protocol developed for M. truncatula R108-1 (c3).
Cosson
Viviane
V
Institut des Sciences du Végétal, CNRS, Gif sur Yvette, Cedex, France.
Durand
Patricia
P
d'Erfurth
Isabelle
I
Kondorosi
Adam
A
Ratet
Pascal
P
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D047108:Embryonic Development / Q000235:genetics; D018014:Gene Transfer Techniques; D046913:Medicago truncatula / Q000196:embryology / Q000235:genetics* / Q000382:microbiology; D018515:Plant Leaves / Q000196:embryology / Q000235:genetics* / Q000382:microbiology; D012038:Regeneration / Q000235:genetics; D014170:Transformation, Genetic