Screening antimicrobial activity of various extracts of Artemisia dracunculus L.
The antimicrobial activities of chloroform, acetone and two different concentrations of methanol extracts of Artemisia dracunculus L. were studied. These extracts were tested against nine bacteria and four yeasts strains by the disc diffusion method. The results indicated that the methanol extract of A. dracunculus is more effective against tested microorganisms than chloroform or acetone extracts. The chloroform and acetone extracts were inhibitory only towards Pseudomonas aeruginosa (ATCC 27853). While the methanol extract that was diluted with 10 ml distilled water showed inhibition zones against Shigella (RSHI), Listeria monocytogenes ATCC 7644, P. aeruginosa (ATCC 27853), the methanol extract that was diluted with 5 ml distilled water showed inhibition zones against two different strains of Escherichia coli (RSHI, ATCC 25922), Shigella (RSHI), L. monocytogenes (ATCC 7644), and P. aeruginosa ATCC 27853. The cells of microorganisms treated with plant extracts and normal microorganism cells were observed by scanning electron microscope. It was apparent that cells are damaged after treatment with A. dracunculus.
Benli
Mehlika
M
Department of Biology, Faculty of Science, University of Ankara, Ankara, Turkey. benli@science.ankara.edu.tr
Kaya
Ilhan
I
Yigit
Nazife
N
D000096:Acetone / Q000737:chemistry; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry*; D002175:Candida / Q000187:drug effects; D002725:Chloroform / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects; D008089:Listeria monocytogenes / Q000187:drug effects; D000432:Methanol / Q000737:chemistry; D008826:Microbial Sensitivity Tests / Q000379:methods; D008855:Microscopy, Electron, Scanning; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D012760:Shigella / Q000187:drug effects; D013045:Species Specificity
D000890:Anti-Infective Agents; D010936:Plant Extracts; D000096:Acetone; D002725:Chloroform; D000432:Methanol