Gene expression profiling of nasopharyngeal carcinoma reveals the abnormally regulated Wnt signaling pathway.
Nasopharyngeal carcinoma (NPC) is a particularly common malignant disease in areas of south China and Southeast Asia. To characterize the gene expression profiling of NPC, we detected the gene expression profiles in 22 NPC and 10 nontumor nasopharyngeal epithelial tissues by complementary DNA microarray. We identified 503 genes that were significantly (P < .001) differentially regulated between NPC and nontumor nasopharyngeal epithelial tissues. The differentially expressed genes are involved in many signaling pathways, such as the Wnt, transforming growth factor-beta, and mitogen-activated protein kinase signaling pathways. The aberrant expression of the Wnt signaling pathway components, such as wingless-type MMTV integration site family, member 5A, Frizzled homolog 7, casein kinase IIbeta, beta-catenin, CREB-binding protein, and Dishevelled-associated activator of morphogenesis 2 was validated on the NPC tissue microarrays. The data suggest that the Wnt signaling pathway may be abnormally regulated in NPC, which provides insight into the molecular mechanisms of NPC.
Zeng
Zhao-Yang
ZY
Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Changsha, Hunan 410078, China.
Zhou
Yan-Hong
YH
Zhang
Wen-Ling
WL
Xiong
Wei
W
Fan
Song-Qing
SQ
Li
Xiao-Ling
XL
Luo
Xiao-Min
XM
Wu
Ming-Hua
MH
Yang
Yi-Xin
YX
Huang
Chen
C
Cao
Li
L
Tang
Ke
K
Qian
Jun
J
Shen
Shou-Rong
SR
Li
Gui-Yuan
GY
D000293:Adolescent; D000328:Adult; D000368:Aged; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism; D047390:Casein Kinase II / Q000235:genetics / Q000378:metabolism; D016000:Cluster Analysis; D005260:Female; D051157:Frizzled Receptors / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics* / Q000502:physiology; D051153:Wnt Proteins / Q000235:genetics* / Q000378:metabolism; D000071818:Wnt-5a Protein; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
C115810:FZD7 protein, human; D051157:Frizzled Receptors; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; D051176:beta Catenin; D050882:CREB-Binding Protein; D047390:Casein Kinase II