title: Kinetic study of cytokines production by human peripheral blood mononuclear cells in response to Brucella DNA.
abstract: In spite of reports on cytokines induction by the Brucella DNA in murine model, there is no comparison between pathogenic and appropriate vaccine strains in human. We investigated the cytokines profile of human peripheral blood mononuclear cells (PBMCs) induced by DNA extracted from pathogenic isolates of Brucella melitensis and B. abortus as well as Rev1 and S19; the appropriate vaccine strains. It was observed that despite differential induction of Interleukin(IL)-12 and IL-10 production, identical IL-12/IL-10 concentration ratio was obtained by all Brucella strains DNAs that was 2 after 24 h and 4 after 5 days of incubation. In addition, IL-2 and Interferon(IFN)-gamma production were profoundly increased compared to the medium at day 3 and 5 respectively but IFN-alpha was not induced. Therefore, Brucella strains DNAs are Th1 inducing component with similar pattern in human PBMCs.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Taghi
authors.initials: T
authors.lastname: Lashkarbolouki
authors.forename: Sussan K
authors.initials: SK
authors.lastname: Ardestani
authors.forename: Amina
authors.initials: A
authors.lastname: Kariminia
authors.forename: Abed-Ali
authors.initials: AA
authors.lastname: Ziaee
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Torkabadi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ebrahimi
mesh_terms: D001769:Blood / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D017347:Brucella melitensis / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004269:DNA, Bacterial / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology
chemical_list: D016207:Cytokines; D004269:DNA, Bacterial