Hairy roots induced by Agrobacterium rhizogenes and production of regenerative plants in hairy root cultures in maize.
Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA. The PCR-Southern hybridization demonstrated that T-DNA had been integrated into the chromosome of regenerated plants.
Xu
Hongwei
H
School of Life Sciences, Northeast Normal University, Changchun 130024, China.
Zhou
Xiaofu
X
Lu
Jingmei
J
Wang
Junjie
J
Wang
Xingzhi
X
D015139:Blotting, Southern; D018517:Plant Roots / Q000502:physiology*; D036741:Plant Tumor-Inducing Plasmids; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012038:Regeneration / Q000502:physiology; D012231:Rhizobium; D003313:Zea mays / Q000235:genetics*