Agrobacterium transformation of Arabidopsis thaliana roots: a quantitative assay.
Arabidopsis thaliana has become a major model system for investigating plant molecular, genetic, and biochemical processes. Arabidopsis is highly susceptible to Agrobacterium-mediated transformation using "flower dip" and "vacuum infiltration" protocols. However, Arabidopsis has also become a major system to investigate the mechanism of Agrobacterium-mediated transformation of somatic tissue. Such investigations require a reproducible, quantitative assay system to determine transformation frequency. We describe here an Arabidopsis root transformation protocol that can be used to determine transformation frequencies for wild-type and mutant Agrobacterium strains and for various Arabidopsis wild-type and mutant lines.
Gelvin
Stanton B
SB
Department of Biological Sciences, Purdue University, West Lafayette, IN, USA.
D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology; D001681:Biological Assay / Q000379:methods; D018014:Gene Transfer Techniques; D009154:Mutation; D018517:Plant Roots / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology; D013045:Species Specificity; D014170:Transformation, Genetic