title: Two families of antimicrobial peptides with multiple functions from skin of rufous-spotted torrent frog, Amolops loloensis.
abstract: There are around 27 species of Amolops amphibian distributed in South-east of Asia. Seven antimicrobial peptides (AMPs) belonging to two different families were purified from skin of rufous-spotted torrent frog, Amolops loloensis, and designated brevinins-ALa, b, c, and d, and temporins-ALa, b, and c. The brevinins-AL family which is structurally related to brevinins-1 from skin secretions of the European frog, Rana brevipoda, is composed of 24 amino acids and has an intra-disulfide bridge at the C-terminus. The temporins-AL family, composed of 13 or 16 amino acid residues, is related with temporins from the skin secretions of R. temporaria. The findings of this study will facilitate the solutions to the taxonomic questions of the ranid genus Amolops and Staurois. In the work of this paper, both brevinins-ALb and temporin-Ma induced mast cell degranulation and histamine release, and had cytotoxic activity toward solid tumor cell line HepG(2). Brevinins-ALb also exerted strong hemolytic activity while temporin-Ma had no such activity.
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: Jianxu
authors.initials: J
authors.lastname: Li
authors.forename: Haining
authors.initials: H
authors.lastname: Yu
authors.forename: Xueqing
authors.initials: X
authors.lastname: Xu
authors.forename: Jianguo
authors.initials: J
authors.lastname: Liang
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Tian
authors.forename: Dongying
authors.initials: D
authors.lastname: Ma
authors.forename: Guoqing
authors.initials: G
authors.lastname: Lin
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Huang
authors.forename: Ren
authors.initials: R
authors.lastname: Lai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D011898:Ranidae; D012867:Skin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D023181:Antimicrobial Cationic Peptides