Radical quenching by rosmarinic acid from Lavandula vera MM cell culture.
This study was conducted to evaluate the radical scavenging capacities of extracts and preparations from a Lavandula vera MM plant cell culture with different rosmarinic acid content and to compare them with pure rosmarinic and caffeic acids as well. The methods, which were used are superoxide anion and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radicals scavenging assays. Results showed that extracts and preparations from Lavandula vera MM possess strong radical scavengers, as the best both radical scavengers appeared to be the fractions with enriched rosmarinic acid content, obtained after ethylacetate fractioning (47.7% inhibition of superoxide radicals and 14.2 microM 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid equivalents, respectively). These data reveal the possibilities for application of these preparations as antioxidants.
Kovacheva
Elena
E
Department of Analytical Chemistry, University of Food Technologies, 26 "Maritza" Blvd., 4002 Plovdiv, Bulgaria. elkovap@yahoo.com
Georgiev
Milen
M
Pashova
Svetlana
S
Angelova
Maria
M
Ilieva
Mladenka
M
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018929:Cell Culture Techniques; D002934:Cinnamates / Q000302:isolation & purification* / Q000494:pharmacology*; D053630:Depsides; D016166:Free Radical Scavengers / Q000494:pharmacology; D027523:Lavandula / Q000737:chemistry* / Q000166:cytology; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D013481:Superoxides; D000097123:Rosmarinic Acid
D000975:Antioxidants; D002934:Cinnamates; D053630:Depsides; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D013481:Superoxides; D000097123:Rosmarinic Acid