Over-expression of genes and proteins of ubiquitin specific peptidases (USPs) and proteasome subunits (PSs) in breast cancer tissue observed by the methods of RFDD-PCR and proteomics.
The ubiquitin-proteasome system facilitates the degradation of damaged proteins and regulators of growth and stress response. Alterations in this proteolytic system are associated with a variety of human pathologies. By restriction fragment differential display polymerase chain reaction (RFDD-PCR) and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS) based on two-dimensional polyacrylamide gel electrophoresis (2-DE), differentially expressed genes and proteins of ubiquitin specific proteases (USPs), proteasome subuinits (PSs) and ubiquitin protein ligase E3A (UBE3A) were analyzed between breast cancer and adjacent normal tissues. Some of them were further verified as over-expression by immunohistochemical stain. Five genes of proteasome subunits (PSs), including PSMB5, PSMD1, PSMD2, PSMD8 and PSMD11, four genes of USPs, including USP9X, USP9Y, USP10 and USP25, and ubiquitin protein ligase E3A (UBE3A) were over-expressed (>3-fold) in breast cancer tissue compared to adjacent normal tissue, and over-expression (>4-fold) of proteins of PSMA1 and SMT3A were observed in breast cancer tissue. PSMD8, PSMD11 and UBE3A were further verified as over-expression by immunohistochemical stain. The action of ubiquitin-proteasome system were obviously enhanced in breast cancer, and selectively intervention in action of ubiquitin-proteasome system may be a useful method of treating human breast cancer.
Deng
Shishan
S
Department of Anatomy, West China School of Preclinical and Forensic Medicine, Sichuan University, Renmin South Road No. 17, Chengdu, China.
Zhou
Hongying
H
Xiong
Ruohong
R
Lu
Youguang
Y
Yan
Dazhong
D
Xing
Tianyong
T
Dong
Lihua
L
Tang
Enjie
E
Yang
Huijun
H
D000328:Adult; D001940:Breast / Q000502:physiology; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D010450:Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D046988:Proteasome Endopeptidase Complex / Q000235:genetics*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D025801:Ubiquitin / Q000235:genetics*; D064570:Ubiquitin-Specific Proteases
D025801:Ubiquitin; D010450:Endopeptidases; D064570:Ubiquitin-Specific Proteases; D046988:Proteasome Endopeptidase Complex