[Reversed phase high-performance liquid chromatography for determination of plasma olanzapine and pharmacokinetics of olanzapine in healthy Chinese volunteers].
OBJECTIVE
To establish a reversed phase high-performance liquid chromatography (HPLC) system for determining plasma concentration of olanzapine and analyze the pharmacokinetics of olanzapine in healthy Chinese volunteers.


METHODS
Ten healthy male subjects received a single oral dose of 20 mg olanzapine tablets. The plasma concentrations of olanzapine were determined by HPLC, and the data were analyzed using 3P97 program.


RESULTS
The plasma concentration curve of olanzapine following a single oral dose conformed to the two compartment open model. The main pharmacokinetics parameters of olanzapine were as follow: C(max) was 113.7-/+33.1 microg/L, T(max) 5.07-/+0.65 h, t(1/2) 35.44-/+4.21 h, AUC(0-144) 2,235-/+257 microg.h.L(-1), and AUC(0-inf) 2,516-/+301 microg.h.L(-1).


CONCLUSIONS
The system established in this study allows for highly sensitive, selective and accurate determination of plasma concentration of olanzapine, and provides valuable information for clinical trials.
Zhu
Jun
J
Department of Pharmacy, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China. dropwatercat@126.com
Yang
Hong
H
Liu
Wei-zhong
WZ
D000328:Adult; D014150:Antipsychotic Agents / Q000097:blood / Q000493:pharmacokinetics; D019540:Area Under Curve; D001569:Benzodiazepines / Q000097:blood* / Q000493:pharmacokinetics*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D000077152:Olanzapine; D015203:Reproducibility of Results
D014150:Antipsychotic Agents; D001569:Benzodiazepines; D000077152:Olanzapine