title: Alternative editing of cytochrome c oxidase III mRNA in trypanosome mitochondria generates protein diversity.
abstract: Trypanosomes use RNA editing to produce most functional mitochondrial messenger RNA. Precise insertion and deletion of hundreds of uridines is necessary to make full-length cytochrome c oxidase III (COXIII) mRNA. We show that COXIII mRNA can be alternatively edited by a mechanism using an alternative guide RNA to make a stable mRNA. This alternatively edited mRNA is translated to produce a unique protein that fractionates with mitochondrial membranes and colocalizes with mitochondrial proteins in situ. Alternative RNA editing represents a previously unknown mechanism generating protein diversity and, as such, represents an important function for RNA editing.
authors.affiliation: Program in Global Infectious Diseases, Josephine Bay Paul Center, Marine Biological Laboratory, 7 MBL Street, Woods Hole, Massachusetts 02543, USA.
authors.forename: Torsten
authors.initials: T
authors.lastname: Ochsenreiter
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Hajduk
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003576:Electron Transport Complex IV / Q000097:blood* / Q000235:genetics; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D017393:RNA Editing; D017394:RNA, Guide, Kinetoplastida / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D000077278:RNA, Mitochondrial; D016053:RNA, Protozoan / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D016053:RNA, Protozoan; C029305:mitochondrial messenger RNA; D003576:Electron Transport Complex IV; D017394:RNA, Guide, Kinetoplastida