title: Genomic cloning of feline Fas ligand gene and characterization of the transcription regulatory region.
abstract: The feline Fas ligand gene was molecularly cloned from a feline genomic library and its genomic organization was determined. The feline Fas ligand gene contained four exons and spanned approximately 10 kb in the genome, and thus had the same structure as the human Fas ligand gene. The promoter region of the feline Fas ligand gene was further characterized by deletion mapping. The region between nucleotides -459 and -172 relative to the ATG codon was essential for the promoter activity when transfected into human and feline lymphoid cells. The characterization of the feline Fas ligand gene in the present study will be useful for further investigations of the regulatory mechanism of feline Fas ligand expression.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan. mizutaku@yamaguchi-u.ac.jp
authors.forename: Takuya
authors.initials: T
authors.lastname: Mizuno
authors.forename: Kenji
authors.initials: K
authors.lastname: Baba
authors.forename: Yuko
authors.initials: Y
authors.lastname: Goto
authors.forename: Kenichi
authors.initials: K
authors.lastname: Masuda
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular; D053222:Fas Ligand Protein / Q000235:genetics* / Q000276:immunology; D015723:Gene Library; D006801:Humans; D019169:Jurkat Cells; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D053222:Fas Ligand Protein