Are there any normal clones?
An exhaustive study of the fidelity of a clone to its parent is prohibitive because of cost and the necessary scope of experimental design. Therefore, these data must be gathered from existing observational evidence. This in itself cannot provide a definitive accounting of the abnormalities and variation found among clones or between clones and parents because there is no standardization in the data points collected between one study and another. This literature survey shows that clone developmental abnormalities, variation among clones, and variation between clone and parent are prevalent at most stages of development (cleavage, placental, fetal, neonatal, maturity), and that occasionally the observed variation greatly exceeds that which might be expected. Some variation can be explained by differences in protocols and procedures between studies. The choice of nuclear donor cell is particularly influential of variation observed between a clone and its parent. In general, however, it appears that there is an inherent stochastic response to nuclear transfer that results in clone infidelity and variation. The survey of characteristics of clone infidelity to parent and documentation of abnormalities provided here should not be viewed as exhaustive or limiting in the recording of such data from future studies. Because controlled hypothesis testing of clone fidelity or clone health may not be possible, meticulous documentation of such observational evidence is a valuable contribution to the field.
Wilmut
Ian
I
Department of Gene Function and Development, Roslin Institute, Roslin, Midlothian, UK.
D000818:Animals; D001757:Blastomeres / Q000502:physiology* / Q000637:transplantation; D002467:Cell Nucleus / Q000502:physiology*; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000009:adverse effects* / Q000379:methods / Q000592:standards; D000013:Congenital Abnormalities; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000002:abnormalities*; D004625:Embryo, Nonmammalian; D005333:Fetus / Q000002:abnormalities; D006262:Health; D053652:Nuclear Transfer Techniques; D010920:Placenta / Q000002:abnormalities