title: [Study on extraction methods of activity components from Podophyllum emodi wall].
abstract: Supercritical fluid Extraction (SFE) with subsequent HPLC analysis was utilized to extract activity components such as podophyllotoxin, deoxypodophyllotoxin, kaempferol and quercetin from the roots and stems of Podophyllum emodi Wall. The extraction yields obtained by SFE were compared to those obtained by organic solvent extraction and were found to be comparable or lower. In addition, we used the orthogonal experiment which conducts with 3 factors and 4 levers in order to find the best dynamic extraction conditions.
authors.affiliation: Key State laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Guang-yuan
authors.initials: GY
authors.lastname: Shen
authors.forename: Xuan
authors.initials: X
authors.lastname: Tian
mesh_terms: D002245:Carbon Dioxide; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification*; D044949:Kaempferols / Q000302:isolation & purification; D000432:Methanol; D010946:Plants, Medicinal / Q000737:chemistry*; D011034:Podophyllotoxin / Q000302:isolation & purification*; D011035:Podophyllum / Q000737:chemistry*; D011312:Pressure; D011794:Quercetin / Q000302:isolation & purification; D027343:Rhizome / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature
chemical_list: D044948:Flavonols; D044949:Kaempferols; D002245:Carbon Dioxide; D011794:Quercetin; D011034:Podophyllotoxin; D000432:Methanol