title: Effect of an enteral diet supplemented with a specific blend of amino acid on plasma and muscle protein synthesis in ICU patients.
abstract: OBJECTIVE
  Polytrauma patients are characterized by a negative nitrogen balance and muscle wasting. Standard nutrition is relatively inefficient to improve muscle protein turnover. The aim of this study was to investigate the effect of enteral nutrition (EN) supplemented with specific amino acids on protein metabolism in polytrauma patients.
  
  
  METHODS
  In a double blind study, 12 polytrauma patients were randomized to receive EN supplemented with either a mixture of cysteine, threonine, serine and aspartate (AA patients) or alanine at isonitrogenous levels (Ala patients). An intravenous infusion of l-[1-(13)C]-leucine was performed in the fed state between day 9 and 12 post-injury (Df) in patients and in a group of healthy volunteers (n=8) (EN+Ala) to measure whole body leucine kinetics, plasma and muscle protein synthesis rates. Nitrogen balance, 3-methyl histidine excretion were measured from day 3 to Df.
  
  
  RESULTS
  The contribution of total plasma proteins to whole body protein synthesis was greatly increased, from 11% in healthy volunteers to about 25% in polytrauma patients. AA supplementation had no effect on nitrogen balance, leucine kinetics or plasma protein synthesis in patients. In contrast, the urinary excretion of 3-methyl histidine tended to decrease along the study in the AA supplemented group compared to an increase in the Ala group. Muscle protein synthesis tended to be higher in the AA group than in the Ala group (46%, P=0.065).
  
  
  CONCLUSIONS
  During injury, an increased supply of cysteine, threonine, serine and aspartate could be able to better cover the specific amino requirements, thus resulting in improved muscle protein synthesis without impairment of acute phase protein synthesis.
authors.affiliation: RCO Département d'anesthésie-réanimation, CHU, 63000 Clermont-Ferrand, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Mansoor
authors.forename: Denis
authors.initials: D
authors.lastname: Breuillé
authors.forename: Fabienne
authors.initials: F
authors.lastname: Béchereau
authors.forename: Caroline
authors.initials: C
authors.lastname: Buffière
authors.forename: Corinne
authors.initials: C
authors.lastname: Pouyet
authors.forename: Bernard
authors.initials: B
authors.lastname: Beaufrère
authors.forename: Jacques
authors.initials: J
authors.lastname: Vuichoud
authors.forename: Martin
authors.initials: M
authors.lastname: Van't-Of
authors.forename: Christiane
authors.initials: C
authors.lastname: Obled
mesh_terms: D000328:Adult; D000368:Aged; D000596:Amino Acids / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism; D001798:Blood Proteins / Q000096:biosynthesis*; D002247:Carbon Isotopes; D003422:Critical Care / Q000379:methods*; D019587:Dietary Supplements; D004311:Double-Blind Method; D004750:Enteral Nutrition / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009124:Muscle Proteins / Q000096:biosynthesis*; D009747:Nutritional Physiological Phenomena; D009751:Nutritional Requirements; D016896:Treatment Outcome; D014947:Wounds and Injuries / Q000378:metabolism / Q000628:therapy*
chemical_list: D000596:Amino Acids; D001798:Blood Proteins; D002247:Carbon Isotopes; D009124:Muscle Proteins