Accelerated apoptosis in SLE neutrophils cultured with anti-dsDNA antibody isolated from SLE patient serum: a pilot study.
Increased numbers of apoptotic neutrophils are found in SLE, related to disease activity and levels of anti-dsDNA antibody. The mechanism of increased apoptosis is not clear, but anti-dsDNA antibody has been shown to induce apoptosis in neutrophils from normal subjects and in certain cell lines. In this study, polyclonal anti-dsDNA antibody was isolated from the serum of a patient with active SLE, and was shown to substantially accelerate apoptosis in neutrophils from SLE patients as compared with neutrophils from healthy control or rheumatoid arthritis subjects.
Armstrong
David J
DJ
Department of Rheumatology, Musgrave Park Hospital, Stockman's Lane, Belfast, BT9 7JB, UK. oswald17727@hotmail.com
Crockard
Alistair D
AD
Wisdom
Brian G
BG
Whitehead
Esme M
EM
Bell
Aubrey L
AL
D000974:Antibodies, Antinuclear / Q000009:adverse effects / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology*; D002478:Cells, Cultured; D004247:DNA / Q000276:immunology; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D010865:Pilot Projects
D000974:Antibodies, Antinuclear; D004247:DNA