title: NMR in the SPINE Structural Proteomics project.
abstract: This paper describes the developments, role and contributions of the NMR spectroscopy groups in the Structural Proteomics In Europe (SPINE) consortium. Focusing on the development of high-throughput (HTP) pipelines for NMR structure determinations of proteins, all aspects from sample preparation, data acquisition, data processing, data analysis to structure determination have been improved with respect to sensitivity, automation, speed, robustness and validation. Specific highlights are protonless (13)C-direct detection methods and inferential structure determinations (ISD). In addition to technological improvements, these methods have been applied to deliver over 60 NMR structures of proteins, among which are five that failed to crystallize. The inclusion of NMR spectroscopy in structural proteomics pipelines improves the success rate for protein structure determinations.
authors.affiliation: Bijvoet Center for Biomolecular Research, NMR Spectroscopy, Utrecht University, Padualaan 8, CH Utrecht, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Ab
authors.forename: A R
authors.initials: AR
authors.lastname: Atkinson
authors.forename: L
authors.initials: L
authors.lastname: Banci
authors.forename: I
authors.initials: I
authors.lastname: Bertini
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authors.initials: S
authors.lastname: Ciofi-Baffoni
authors.forename: K
authors.initials: K
authors.lastname: Brunner
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authors.lastname: Diercks
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authors.lastname: Dötsch
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authors.initials: F
authors.lastname: Engelke
authors.forename: G E
authors.initials: GE
authors.lastname: Folkers
authors.forename: C
authors.initials: C
authors.lastname: Griesinger
authors.forename: W
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authors.lastname: Gronwald
authors.forename: U
authors.initials: U
authors.lastname: Günther
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authors.lastname: Habeck
authors.forename: R N
authors.initials: RN
authors.lastname: de Jong
authors.forename: H R
authors.initials: HR
authors.lastname: Kalbitzer
authors.forename: B
authors.initials: B
authors.lastname: Kieffer
authors.forename: B R
authors.initials: BR
authors.lastname: Leeflang
authors.forename: S
authors.initials: S
authors.lastname: Loss
authors.forename: C
authors.initials: C
authors.lastname: Luchinat
authors.forename: T
authors.initials: T
authors.lastname: Marquardsen
authors.forename: D
authors.initials: D
authors.lastname: Moskau
authors.forename: K P
authors.initials: KP
authors.lastname: Neidig
authors.forename: M
authors.initials: M
authors.lastname: Nilges
authors.forename: M
authors.initials: M
authors.lastname: Piccioli
authors.forename: R
authors.initials: R
authors.lastname: Pierattelli
authors.forename: W
authors.initials: W
authors.lastname: Rieping
authors.forename: T
authors.initials: T
authors.lastname: Schippmann
authors.forename: H
authors.initials: H
authors.lastname: Schwalbe
authors.forename: G
authors.initials: G
authors.lastname: Travé
authors.forename: J
authors.initials: J
authors.lastname: Trenner
authors.forename: J
authors.initials: J
authors.lastname: Wöhnert
authors.forename: M
authors.initials: M
authors.lastname: Zweckstetter
authors.forename: R
authors.initials: R
authors.lastname: Kaptein
mesh_terms: D000465:Algorithms; D003627:Data Interpretation, Statistical; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins