Bioassay-guided isolation of antiatherosclerotic phytochemicals from Artocarpus altilis.
The cytoprotective effects of various solvent extracts of Artocarpus altilis (Parkinson) Fosberg were evaluated. The cytoprotective effects were determined in human U937 cells incubated with oxidized LDL (OxLDL) using the 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1, 3-benzene disulfonate (WST-1) assay. The results demonstrated that the ethyl acetate extract showed cytoprotective activities. To identify the main cytoprotective components, a bioassay guided isolation of the ethyl acetate extract afforded b-sitosterol (1) and six flavonoids (2-7). Their chemical structures were established on the basis of spectroscopic evidence and comparison with literature data. Of these compounds, compound 6 was obtained from A. altilis for the first time. The cytoprotective effect offers good prospects for the medicinal applications of A. altilis.
Wang
Yu
Y
Department of Biomedical Engineering, Key Laboratory for Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, PR China.
Deng
Tongle
T
Lin
Lin
L
Pan
Yuanjiang
Y
Zheng
Xiaoxiang
X
D031622:Artocarpus / Q000737:chemistry*; D050197:Atherosclerosis / Q000188:drug therapy / Q000517:prevention & control; D001681:Biological Assay / Q000379:methods; D002470:Cell Survival / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D012855:Sitosterols / Q000737:chemistry* / Q000302:isolation & purification; D020298:U937 Cells
D005419:Flavonoids; D008077:Lipoproteins, LDL; D010936:Plant Extracts; D012855:Sitosterols; C099252:oxidized low density lipoprotein; C025473:gamma-sitosterol