The use of siRNA to validate immunofluorescence studies.
Cellular immunofluorescence studies can be validated by using either specific small interfering RNA (siRNA) duplexes or expression plasmids that induce the expression of specific siRNAs. The usage of either siRNA tool reduces the expression of the specific protein being studied, thus reducing substantially or abolishing the immunofluorescence detected when using a fluorescent antibody that recognizes the protein.
Moore
K Gregory
KG
Serologicals Corporation, Charlottesville, VA, USA.
Speckmann
Wayne
W
Herzig
Ronald P
RP
D005455:Fluorescent Antibody Technique / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids / Q000235:genetics; D034741:RNA, Small Interfering / Q000378:metabolism*; D015203:Reproducibility of Results; D014162:Transfection
D034741:RNA, Small Interfering