title: SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects.
abstract: This paper reviews the developments in high-throughput and nanolitre-scale protein crystallography technologies within the remit of workpackage 4 of the Structural Proteomics In Europe (SPINE) project since the project's inception in October 2002. By surveying the uptake, use and experience of new technologies by SPINE partners across Europe, a picture emerges of highly successful adoption of novel working methods revolutionizing this area of structural biology. Finally, a forward view is taken of how crystallization methodologies may develop in the future.
authors.affiliation: Division of Structural Biology and Oxford Protein Production Facility (OPPF), Wellcome Trust Centre for Human Genetics, Oxford, England.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Berry
authors.forename: O
authors.initials: O
authors.lastname: Dym
authors.forename: R M
authors.initials: RM
authors.lastname: Esnouf
authors.forename: K
authors.initials: K
authors.lastname: Harlos
authors.forename: R
authors.initials: R
authors.lastname: Meged
authors.forename: A
authors.initials: A
authors.lastname: Perrakis
authors.forename: J L
authors.initials: JL
authors.lastname: Sussman
authors.forename: T S
authors.initials: TS
authors.lastname: Walter
authors.forename: J
authors.initials: J
authors.lastname: Wilson
authors.forename: Albrecht
authors.initials: A
authors.lastname: Messerschmidt
mesh_terms: D003461:Crystallography / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D007091:Image Processing, Computer-Assisted; D036103:Nanotechnology; D010969:Plastics; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics; D011786:Quality Control; D012371:Robotics
chemical_list: D010969:Plastics; D011506:Proteins