title: Effect of soluble fiber or fructooligosaccharide supplementation upon trinitrobenzenesulphonic acid induced colitis in rats.
abstract: OBJECTIVE
  To evaluate the effect of soluble fiber or fructooligosaccharide (FOS) supplementation upon trinitrobenzene sulphonic acid (TNBS)-induced colitis in rats.
  
  
  METHODS
  64 Wistar rats were given water, soluble fiber or FOS intragastrically during 14 days prior to colitis induction with TNBS (n=48) or rectal enema with water (n=16; control group). On the 7th or 14th day following colitis induction the rats were weighed and euthanized in order to determine the colon weight/length ratio and macroscopic and microscopic scores.
  
  
  RESULTS
  On the 7th day following colitis induction the body weight had decreased significantly, the colon weight/length ratio had increased and macroscopic and microscopic colon lesions were observed. On the 14th day following colitis induction no difference in body weight was observed, in spite of the persistence of macroscopic and microscopic lesions and increased colon weight/length ratio. Supplementation with soluble fiber or FOS did not revert colon lesions or any of the study parameters. Supplementation with FOS, but not with fiber, was associated with increased colon weight/length ratio on the 14th day.
  
  
  CONCLUSIONS
  Supplementation with soluble fiber or FOS produced no significant impact on TNBS-induced colitis in rats.
authors.affiliation: Department of Surgery, Faculty of Medicine, Federal University of Ceará (UFC), Tocantins, Brazil. smregadas@hospitalsaocarlos.com.br
authors.forename: Sthela Maria
authors.initials: SM
authors.lastname: Murad-Regadas
authors.forename: Marcellus Henrique Loiola Ponte de
authors.initials: MH
authors.lastname: Souza
authors.forename: Gerly Anne de Castro
authors.initials: GA
authors.lastname: Brito
authors.forename: Lusmar Veras
authors.initials: LV
authors.lastname: Rodrigues
authors.forename: Francisco Sérgio Pinheiro
authors.initials: FS
authors.lastname: Regadas
authors.forename: Paulo Roberto Leitão de
authors.initials: PR
authors.lastname: Vasconcelos
mesh_terms: D000284:Administration, Oral; D000285:Administration, Rectal; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D003106:Colon / Q000187:drug effects / Q000473:pathology; D004043:Dietary Fiber / Q000627:therapeutic use*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D008297:Male; D009844:Oligosaccharides / Q000627:therapeutic use*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D013997:Time Factors; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D004043:Dietary Fiber; D009844:Oligosaccharides; C116580:fructooligosaccharide; D014302:Trinitrobenzenesulfonic Acid