title: Human gingival fibroblast response to electropolished NiTi surfaces.
abstract: In the present study the in vitro biocompatibility of electropolished NiTi sheets is investigated. The assessment of cytotoxic effects due to potential Ni leaching from metal sheets was performed in direct contact with primary human fibroblast cultures using the 5-bromo-2'-deoxyuridine cell proliferation assay and morphologic studies via light microscopy and scanning electron microscopy. To assess toxic effects related to Ni-ions release, cells cultured in the presence of increasing concentrations of Ni(2+) (NiSO(4).6H(2)O) served as positive controls. It is shown that while the addition of NiSO(4) caused severe proliferation decrease (approximately 80%) and morphologic damage at a concentration of 50 mg/L Ni(2+) no negative effects were observed in fibroblasts cultured in the presence of electropolished NiTi sheets. The results are discussed in terms of surface topography effects on the biocompatibility of NiTi shape memory alloys.
authors.affiliation: Department of Orthodontics, University Clinic Schleswig-Holstein (UKSH), Christian-Albrechts University of Kiel, Kiel, Germany. es-souni@kfo-zmk.uni-kiel.de
authors.forename: Martha
authors.initials: M
authors.lastname: Es-Souni
authors.forename: Helge
authors.initials: H
authors.lastname: Fischer-Brandies
authors.forename: Mohammed
authors.initials: M
authors.lastname: Es-Souni
mesh_terms: D000497:Alloys; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D007509:Irritants / Q000494:pharmacology; D008422:Materials Testing; D009532:Nickel / Q000494:pharmacology; D013499:Surface Properties; D014025:Titanium
chemical_list: D000497:Alloys; D007509:Irritants; C029938:nickel sulfate; D009532:Nickel; D014025:Titanium