Improved glucuronide hydrolysis in the determination of urinary 1-hydroxypyrene.
The efficiency of the glucuronide hydrolysis in the determination of urinary 1-hydroxypyrene was investigated as a function of the reaction conditions. A significant improvement could be obtained by increasing the enzyme concentration described in the literature.
Wegener
J W M
JW
Vrije Universiteit Amsterdam, Institute for Environmnetal Studies, De Boelelaan 1087, NL-1081HV Amsterdam, Netherlands. jan.willem.wegener@ivm.vu.nl
Hopman-Ubbels
G H J
GH
Van Velzen
M
M
D005966:Glucuronidase / Q000378:metabolism; D020719:Glucuronides / Q000378:metabolism* / Q000652:urine*; D006801:Humans; D006868:Hydrolysis; D011721:Pyrenes / Q000032:analysis* / Q000302:isolation & purification
D020719:Glucuronides; D011721:Pyrenes; D005966:Glucuronidase; C033146:1-hydroxypyrene