title: Sequence and function of canine herpesvirus alpha-transinducing factor and its interaction with an immediate early promoter.
abstract: The sequence of the alpha-transinducing factor (alpha-TIF) of canine herpesvirus (CHV-l) was determined. Alignment of the predicted CHV-1 alpha-TIF amino acid sequence with other alpha-TIF homologues reveals a core region of similarity with divergent amino and carboxyl termini. Analysis of the CHV-1 infected cell protein 4 promoter region identified a region containing nine copies of a 52 bp repeat that showed significant up-regulation of transcription by alpha-TIF. This region contained an imperfect 'TAATGARAT' motif, the binding site for herpes simplex virus 1 alpha-TIF, with an imperfect Oct-1 binding site immediately following. The infectious laryngotracheitis virus alpha-TIF was also shown to up-regulate transcription through this region of the promoter. Transfection of CHV-1 genomic DNA failed to yield infectious virus in canine kidney cell lines. Co-transfection of genomic DNA and an alpha-TIF expression plasmid resulted in virus plaques, indicating a potential essential role for alpha-TIF in CHV-1 infection.
authors.affiliation: CSIRO Livestock Industries, Australian Animal Health Laboratory, Private Bag No. 24, Geelong, VIC., 3220, Australia. scott.tyack@csiro.au
authors.forename: Scott G
authors.initials: SG
authors.lastname: Tyack
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Studdert
authors.forename: Michael A
authors.initials: MA
authors.lastname: Johnson
mesh_terms: D000818:Animals; D004285:Dogs; D015967:Gene Expression Regulation, Viral; D017781:Genes, Immediate-Early / Q000502:physiology*; D005814:Genes, Viral; D002184:Herpesvirus 1, Canid / Q000737:chemistry*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D014157:Transcription Factors