Histone H2A as a transfection agent in crayfish hematopoietic tissue cells.
We report a novel and highly efficient dsRNA transfection system based on one of the nuclear proteins, namely, histone H2A. RT-PCR semi-quantitative analysis of silencing target gene shows that the transfection efficiency of histone H2A is higher than Effectene or liposome-based transfection systems. Importantly, the high efficiency of histone H2A was associated with very low toxicity to the transfected crayfish hematopoietic tissue (Hpt) cells. The non-toxicity, effectiveness and specificity of histone H2A as a transfection agent provides a cheap, simple, highly efficient and reproducible gene delivery system, particularly for the sensitive cell cultures of crustacean animals such as crayfish and shrimp.
Liu
Haipeng
H
Department of Comparative Physiology, Uppsala University, Norbyvägen 18A, Uppsala 752 36, Sweden.
Söderhäll
Irene
I
D000818:Animals; D003400:Astacoidea / Q000166:cytology / Q000502:physiology*; D006410:Hematopoiesis / Q000235:genetics*; D006657:Histones / Q000235:genetics*; D034622:RNA Interference; D014162:Transfection
D006657:Histones