[Determination of monosaccharides in Sargassum hemiphyllum (Turner) C. Ag. polysaccharides by ion chromatography].
Sargassum hemiphyllum polysaccharides (SHP) was extracted from dry Sargassum hemiphyllum (Turner) C. Ag. powder using 60 - 80 degrees C purified water and then hydrolyzed with 4.0 g/L trifluoroacetic acid at 80 degrees C. Without any derivatization reaction, the determination of monosaccharides in SHP was developed by anion-exchange chromatography with pulsed amperometric detection with an Au working electrode and an Ag/AgCl reference electrode. Monosaccharides were separated on a CarboPac PA10 anion-column (2 mm i. d. x 250 mm) by using isocratic elution consisting of 14 mmol/L sodium hydroxide at a flow rate of 0.20 mL/min. Six monosaccharides, xylose, galactose, arabinose, glucose, rhamnose and fructose, contained in SHP were separated and determined. Their contents in SHP were 2 200, 820, 98, 4 560, 358 and 740 mg/kg, respectively. The recoveries of the six monosaccharides were in the range 86.0% - 108.0%. The detection limits for these monosaccharides ranged from 5.6 to 89.6 microg/kg. The experimental results showed that SHP mainly consisted of xylose and glucose with smaller quantities of galactose, arabinose, rhamnose and fructose. This method is suitable for the determination of monosaccharides without any derivatization reaction at the level of microg/kg in dry algae with high sensitivity and good precision.
Ou
Yunfu
Y
Center of Experimental Technology, Jinan University, Guangzhou 510632, China.
Yin
Pinghe
P
Zhao
Ling
L
D002845:Chromatography; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004566:Electrodes / Q000706:statistics & numerical data; D007477:Ions / Q000737:chemistry; D009005:Monosaccharides / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D044647:Sargassum / Q000737:chemistry*
D007477:Ions; D009005:Monosaccharides; D011134:Polysaccharides