Microarray analysis of the developing cortex.
Abnormal development of the prefrontal cortex (PFC) is associated with a number of neuropsychiatric disorders that have an onset in childhood or adolescence. Although the basic laminar structure of the PFC is established in utero, extensive remodeling continues into adolescence. To map the overall pattern of changes in cortical gene transcripts during postnatal development, we made serial measurements of mRNA levels in mouse PFC using oligonucleotide microarrays. We observed changes in mRNA transcripts consistent with known postnatal morphological and biochemical events. Overall, most transcripts that changed significantly showed a progressive decrease in abundance after birth, with the majority of change between postnatal weeks 2 and 4. Genes with cell proliferative, cytoskeletal, extracellular matrix, plasma membrane lipid/transport, protein folding, and regulatory functions had decreases in mRNA levels. Quantitative PCR verified the microarray results for six selected genes: DNA methyltransferase 3A (Dnmt3a), procollagen, type III, alpha 1 (Col3a1), solute carrier family 16 (monocarboxylic acid transporters), member 1 (Slc16a1), MARCKS-like 1 (Marcksl1), nidogen 1 (Nid1) and 3-hydroxybutyrate dehydrogenase (heart, mitochondrial) (Bdh).
Semeralul
Mawahib O
MO
Department of Pharmacology, Faculty of Medicine, University of Toronto, Ontario, Canada.
Boutros
Paul C
PC
Likhodi
Olga
O
Okey
Allan B
AB
Van Tol
Hubert H M
HH
Wong
Albert H C
AH
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D016000:Cluster Analysis; D000090205:DNA Methyltransferase 3A; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D046228:Microarray Analysis / Q000379:methods*; D017397:Prefrontal Cortex / Q000254:growth & development* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
C000715535:Dnmt3a protein, mouse; D012333:RNA, Messenger; D000090205:DNA Methyltransferase 3A