Effect of cytoplast on the development of inter-subspecies nuclear transfer reconstructed goat embryo.
The aim of this study was to investigate effect of cytoplast on the development competence of reconstructed embryos derived from inter-subspecies somatic cell nucleus transfer (SCNT). First, the development potency of reconstructed embryos produced by transferring Boer goat fibroblast cell nucleus of different ages into enucleated Sannen goat ova was evaluated in order to determine which age of nuclear donor is favorable for the reconstructed embryos development. Secondly, the another component of reconstructed embryos, "cytoplast," was evaluated by comparing the effect of ovum cytoplast derived from Sannen male symbol x Boer female symbol descendant on the reconstructed embryos development to that of Sannen goat ovum cytoplast. The results revealed that the development rate of the reconstructed embryos derived from 2 months old Boer goat somatic cells was the highest, their gestation rate was up to 50%, and one viable male offspring was obtained. The cytoplast derived from the crossbreeding goats improves the development competence of reconstructed embryos, which birth rate was 5.5%. The genetic identification of offspring by using PCR-SSCP analysis confirmed that these cloned kids were derived from the donor. The results above reveal that the cytoplast of Sannen goat ovum could induce the dedifferentiation of somatic cell nuclei derived from Boer goat, but the reprogramming process of these reconstructed embryos seems incomplete, probably due to some incorrect processes happened after implantation. Relatedness components of nucleus donor in cytoplast of the crossbreeding goat may be helpful to induce the dedifferentiation of somatic cell nuclei completely and improve the development competence of the reconstructed embryos.
Jian-Quan
Chen
C
School of Life Science and Technology, Tongji University, Shanghai, China.
Juan
Chen
C
Xu-Jun
Xu
X
Guo-Hui
Liu
L
Si-Guo
Liu
L
Hong-Ying
Sha
S
You-Bing
Wu
W
Guo-Xiang
Cheng
C
D000818:Animals; D002454:Cell Differentiation; D002467:Cell Nucleus; D019976:Cloning, Organism; D003593:Cytoplasm / Q000502:physiology*; D047108:Embryonic Development; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006041:Goats / Q000196:embryology* / Q000235:genetics; D006824:Hybridization, Genetic; D066298:In Vitro Techniques; D008297:Male; D053652:Nuclear Transfer Techniques; D010063:Ovum / Q000166:cytology* / Q000502:physiology; D011110:Polymorphism, Genetic