title: Establishment of a gene expression system in Ochrobactrum anthropi.
abstract: Genetic studies of Ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. We constructed a set of vectors containing several promoters and a His tag fusion in the N terminus to facilitate protein detection and purification. The new vectors should significantly enhance the genetic manipulation and characterization of O. anthropi.
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic and State University, Blacksburg, VA 24060, USA.
authors.forename: Mohamed N
authors.initials: MN
authors.lastname: Seleem
authors.forename: Mohammed
authors.initials: M
authors.lastname: Ali
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Boyle
authors.forename: Biswarup
authors.initials: B
authors.lastname: Mukhopadhyay
authors.forename: Sharon G
authors.initials: SG
authors.lastname: Witonsky
authors.forename: Gerhardt G
authors.initials: GG
authors.lastname: Schurig
authors.forename: Nammalwar
authors.initials: N
authors.lastname: Sriranganathan
mesh_terms: D015870:Gene Expression / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005798:Genes, Bacterial / Q000502:physiology; D008969:Molecular Sequence Data; D020621:Ochrobactrum anthropi / Q000235:genetics* / Q000378:metabolism