title: Differentiated cells are more efficient than adult stem cells for cloning by somatic cell nuclear transfer.
abstract: Since the creation of Dolly via somatic cell nuclear transfer (SCNT), more than a dozen species of mammals have been cloned using this technology. One hypothesis for the limited success of cloning via SCNT (1%-5%) is that the clones are likely to be derived from adult stem cells. Support for this hypothesis comes from the findings that the reproductive cloning efficiency for embryonic stem cells is five to ten times higher than that for somatic cells as donors and that cloned pups cannot be produced directly from cloned embryos derived from differentiated B and T cells or neuronal cells. The question remains as to whether SCNT-derived animal clones can be derived from truly differentiated somatic cells. We tested this hypothesis with mouse hematopoietic cells at different differentiation stages: hematopoietic stem cells, progenitor cells and granulocytes. We found that cloning efficiency increases over the differentiation hierarchy, and terminally differentiated postmitotic granulocytes yield cloned pups with the greatest cloning efficiency.
authors.affiliation: Center for Regenerative Biology and Department of Animal Science, University of Connecticut, Storrs, Connecticut 06269, USA.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Sung
authors.forename: Shaorong
authors.initials: S
authors.lastname: Gao
authors.forename: Hongmei
authors.initials: H
authors.lastname: Shen
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.forename: Yifang
authors.initials: Y
authors.lastname: Song
authors.forename: Sadie L
authors.initials: SL
authors.lastname: Smith
authors.forename: Ching-Chien
authors.initials: CC
authors.lastname: Chang
authors.forename: Kimiko
authors.initials: K
authors.lastname: Inoue
authors.forename: Lynn
authors.initials: L
authors.lastname: Kuo
authors.forename: Jin
authors.initials: J
authors.lastname: Lian
authors.forename: Ao
authors.initials: A
authors.lastname: Li
authors.forename: X Cindy
authors.initials: XC
authors.lastname: Tian
authors.forename: David P
authors.initials: DP
authors.lastname: Tuck
authors.forename: Sherman M
authors.initials: SM
authors.lastname: Weissman
authors.forename: Xiangzhong
authors.initials: X
authors.lastname: Yang
authors.forename: Tao
authors.initials: T
authors.lastname: Cheng
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D019976:Cloning, Organism / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D020869:Gene Expression Profiling; D006098:Granulocytes / Q000166:cytology / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008954:Models, Biological; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology / Q000502:physiology