[Study on the distribution of lactase in the small intestine of SD rats].
OBJECTIVE
The article deals with the study on the distribution of lactase and its mRNA in the SD rat intestine.


METHODS
15 of the 3-4 wk old SD rats were selected for this research project. From SD rat duodenum, we scissored the small intestine into 3 segments of each 10 cm length to determine the lactase activities and mRNA levels.


RESULTS
The lactase activities of all 3 part intestines were declined from the duodenum stage by stage (the upper, middle and lower segment was 0.179, 0.160, or 0.151 U/ml homogenate respectively, P < 0.05), but there were no significant difference of mRNA levels happening among the 3 groups.


CONCLUSIONS
The results suggest that the lactase activity mainly distribute over the upper intestine, although there were lactase along whole intestine.
He
Wei
W
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu.
Lü
Bin
B
Huang
Cheng-yu
CY
D000818:Animals; D005260:Female; D007421:Intestine, Small / Q000201:enzymology*; D043322:Lactase / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D012333:RNA, Messenger; D043322:Lactase