[Analysis of volatile constituents from leaves of plants by gas chromatography/mass spectrometry with solid-phase microextraction].
A method for analyzing volatile constituents from plant leaves with gas chromatography/mass spectrometry (GC/MS) coupled with solid-phase microextraction (SPME) was developed. The volatile compounds from the plant leaves inside a sealed flask maintained at 45 degrees C in a water bath were efficiently extracted with Polyacrylate (85 microm) SPME fibers prior to perform GC/MS analysis. The GC/MS analysis indicated that the volatile compounds from the plant leaves which is easy to be damaged by Tetraychus vienneis include relative large amounts of cis-3-hexenyl ester acetic acid, cis-3-hexenyl ester butanoic acid and alpha-famesene. These compounds were preliminarily confirmed to be accountable for attracting Tetraychus vienneis. This finding may lead to identify biological species for preventing and treating Tetraychus vienneis.
Wang
Minglin
M
College of Food Science, Shandong Agricultural University, Taian 271018, China. mlwang@sdau.edu.cn
Qiao
Luqin
L
Zhang
Li
L
Wu
Liejun
L
Tian
Hongxiao
H
D019411:Clinical Laboratory Techniques; D035264:Flowers / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008872:Microwaves; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D010938:Plant Oils / Q000032:analysis / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry*; D010944:Plants / Q000737:chemistry; D012015:Reference Standards; D052617:Solid Phase Microextraction / Q000379:methods; D013696:Temperature; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000494:pharmacology; D014835:Volatilization
D010936:Plant Extracts; D010938:Plant Oils; D055549:Volatile Organic Compounds