Langerhans cells in cervical intraepithelial neoplasia related to smoking habits.
OBJECTIVE
Compare the numerical densities of intraepithelial Langerhans cells of uterine cervix of women affected by cervical intraepithelial neoplasia grade 3 (CIN 3) with their smoking habits.


METHODS
A total of 71 conization specimens of women affected by CIN 3 were separated in 3 groups according to their smoking habits (smokers, nonsmokers, and former smokers). The identification of the Langerhans cells was performed by immunohistochemical analysis using antibodies to S100 protein. The number of intraepithelial Langerhans cells was counted at x400 magnification under a light microscope, and a 10-field count was performed in areas of CIN 3 of each section. Results were expressed as number of cells per square millimeter of epithelium.


RESULTS
There was no significant difference in the number of Langerhans cells per square millimeter of epithelium in areas affected by CIN 3 among the 3 groups (p = .5). There was also no significant difference in the number of cigarettes smoked per day (p = .09), duration of consumption (p = .34), total amount of cigarettes smoked during the whole life (p = .18), and duration of abstention (p = .2).


CONCLUSIONS
It was not shown that smoking reduces the number of intraepithelial Langerhans cells in the cervix of women affected by CIN 3.
Campaner
Adriana Bittencourt
AB
Department of Obstetrics and Gynecology, Medical Science College of Santa Casa of São Paulo, Brazil. abcampaner@terra.com.br
Piato
Sebastião
S
Galvão
Maria Antonieta L
MA
dos Santos
Roberto Euzébio
RE
Nadais
Ricardo F
RF
D000293:Adolescent; D000328:Adult; D000368:Aged; D002452:Cell Count; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000473:pathology*; D008875:Middle Aged; D009367:Neoplasm Staging; D012189:Retrospective Studies; D012307:Risk Factors; D009418:S100 Proteins / Q000276:immunology; D012907:Smoking; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000276:immunology / Q000473:pathology*; D002578:Uterine Cervical Dysplasia / Q000209:etiology / Q000276:immunology / Q000473:pathology*
D009418:S100 Proteins