Analysis of antibody response in humans to the type A OspC loop 5 domain and assessment of the potential utility of the loop 5 epitope in Lyme disease vaccine development.
The OspC protein of Borrelia burgdorferi is an immunodominant antigen. Here we demonstrate that the loop 5 domain of type A OspC is surface exposed, elicits bactericidal antibody in mice, and is antigenic in humans. The data suggest that loop 5 may be suitable for inclusion in a polyvalent, chimeric OspC vaccinogen.
Buckles
Eric L
EL
Department of Microbiology and Immunology, Medical College of Virginia at Virginia Commonwealth University, 1112 E. Clay St., McGuire Hall, Richmond, VA 23298-0678, USA.
Earnhart
Christopher G
CG
Marconi
Richard T
RT
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D008193:Lyme Disease / Q000276:immunology* / Q000382:microbiology; D022123:Lyme Disease Vaccines / Q000138:chemical synthesis / Q000276:immunology*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D022123:Lyme Disease Vaccines; C079616:OspC protein