Protein A chromatography for antibody purification.
Staphylococcal protein A (SPA) is one of the first discovered immunoglobulin binding molecules and has been extensively studied during the past decades. Due to its affinity to immunoglobulins, SPA has found widespread use as a tool in the detection and purification of antibodies and the molecule has been further developed to one of the most employed affinity purification systems. Interestingly, a minimized SPA derivative has been constructed and a domain originating from SPA has been improved to withstand the harsh environment employed in industrial purifications. This review will focus on the development of different affinity molecules and matrices for usage in antibody purification.
Hober
Sophia
S
Royal Institute of Technology, Stockholm, Sweden.
Nord
Karin
K
Linhult
Martin
M
D000906:Antibodies / Q000737:chemistry / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D015202:Protein Engineering / Q000379:methods; D013205:Staphylococcal Protein A / Q000737:chemistry*
D000906:Antibodies; D013205:Staphylococcal Protein A