Direct evidence for a critical role of CD30 in the development of allergic asthma.
BACKGROUND
CD30 is a costimulatory molecule belonging to the TNF receptor superfamily that is expressed on activated T and B cells. Several studies have demonstrated a positive correlation between expression of CD30 or increased levels of soluble CD30 and the development and severity of allergic diseases. However, thus far, the evidence for a role of CD30 in allergic diseases, such as asthma, is only indirect.


OBJECTIVE
The aim of the study was to directly investigate the role of CD30 in a murine asthma model.


METHODS
CD30-deficient (B6.129P2-Tnfrsf8(tm1Mak)/J) and wild-type (WT) mice were immunized to ovalbumin (OVA) to induce an asthma-like phenotype and compared in our murine asthma model. Moreover, CD30/CD30 ligand signaling was blocked in OVA-immunized WT animals by using mAbs against CD30 receptor and its ligand, CD153.


RESULTS
The absence of CD30 in OVA-immunized CD30-deficient mice resulted in significantly reduced airway inflammation, serum IgE levels, and TH2 cytokine levels. The same effect was observed when CD30/CD153 signaling was blocked in OVA-immunized WT animals with mAbs against CD30 or CD30 ligand.


CONCLUSIONS
Our results directly demonstrate that CD30/CD153 interaction plays an important role in the induction of TH2 cell-mediated allergic asthma.


CONCLUSIONS
These findings provide evidence for a role of the costimulatory molecule CD30 in allergic asthma.
Polte
Tobias
T
Department of Pediatrics, Division of Pediatric Pulmonology and Neonatology, Medizinische Hochschule Hannover, Germany.
Behrendt
Ann-Kathrin
AK
Hansen
Gesine
G
D000818:Animals; D001249:Asthma / Q000276:immunology* / Q000473:pathology; D053285:CD30 Ligand / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D005434:Flow Cytometry; D007073:Immunoglobulin E / Q000097:blood; D017730:Ki-1 Antigen / Q000276:immunology* / Q000378:metabolism*; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008817:Mice, Mutant Strains; D010047:Ovalbumin / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
D053285:CD30 Ligand; D016207:Cytokines; D017730:Ki-1 Antigen; D007073:Immunoglobulin E; D010047:Ovalbumin