title: Effect of a conjugated quercetin metabolite, quercetin 3-glucuronide, on lipid hydroperoxide-dependent formation of reactive oxygen species in differentiated PC-12 cells.
abstract: To assess the efficacy of conjugated quercetin metabolites as attenuators for oxidative stress in the central nervous system, we measured the 13-hydroperoxyoctadecadienoic acid (13-HPODE)-dependent formation of reactive oxygen species (ROS) in pheochromocytoma PC-12 cells in the presence of quercetin 3-O-beta-glucuronide (Q3GA) and related compounds. A 2',7'-dichlorofluorescin (DCFH) assay showed that Q3GA significantly suppressed the formation of ROS, when it was coincubated with 13-HPODE (coincubation system). However, it was less effective than quercetin aglycon in the concentration range from 0.5 to 10 microM. In an experiment in which the cells were incubated with the test compounds for 24 h before being exposed to 13-HPODE, Q3GA was also effective in suppressing the formation of ROS in spite that little Q3GA was taken up into the cells. These results suggest that antioxidative metabolites of quercetin are capable of protecting nerve cells from attack of lipid hydroperoxides.
authors.affiliation: Department of Food Science, Institute for Health Biosciences, The University of Tokushima Graduate School, Kuramoto-cho 3, Tokushima 770-8503, Japan.
authors.forename: Mutsuko
authors.initials: M
authors.lastname: Shirai
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Kawai
authors.forename: Rintaro
authors.initials: R
authors.lastname: Yamanishi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kinoshita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Chuman
authors.forename: Junji
authors.initials: J
authors.lastname: Terao
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D016166:Free Radical Scavengers / Q000378:metabolism / Q000494:pharmacology*; D008041:Linoleic Acids / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000633:toxicity; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000378:metabolism / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D011794:Quercetin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D008041:Linoleic Acids; D008054:Lipid Peroxides; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C443401:quercetin 3-O-glucuronide; C038649:13-hydroperoxy-9,11-octadecadienoic acid; D011794:Quercetin