title: Rabbit antithymocyte globulin inhibits monocyte-derived dendritic cells maturation in vitro and polarizes monocyte-derived dendritic cells towards tolerogenic dendritic cells expressing indoleamine 2,3-dioxygenase.
abstract: BACKGROUND
  Rabbit antithymocyte globulin (rATG) is a polyclonal mixture of immunoglobulin (Ig) G. It is used to prevent graft rejection and also graft versus host disease after transplantation. Its effect on lymphocyte function has been widely studied. Dendritic cells are central actors of the immune system. As antigen presenting cells, they are able to initiate, stimulate, and modulate immune responses.
  
  
  METHODS
  In this study, we investigated rATG effects on in vitro differentiation and maturation of monocyte-derived dendritic cells (Mo-DCs).
  
  
  RESULTS
  rATG inhibited maturation of immature Mo-DCs and allowed the generation of dendritic cells expressing ILT-3, CD123, CCR6 but not CCR7 and producing Indoleamine 2,3-dioxygenase mRNA as well as interferon-alpha.
  
  
  CONCLUSIONS
  rATG polarizes in vitro Mo-DCs towards tolerogenic dendritic cells.
authors.affiliation: Etablissement Français du Sang, Laboratoire d'Immunologie, Lyon, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Gillet-Hladky
authors.forename: Camila Miranda
authors.initials: CM
authors.lastname: de Carvalho
authors.forename: Janine
authors.initials: J
authors.lastname: Bernaud
authors.forename: Christine
authors.initials: C
authors.lastname: Bendahou
authors.forename: Christian
authors.initials: C
authors.lastname: Bloy
authors.forename: Dominique
authors.initials: D
authors.lastname: Rigal
mesh_terms: D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D005434:Flow Cytometry; D006084:Graft Rejection / Q000517:prevention & control; D006086:Graft vs Host Disease / Q000517:prevention & control; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007108:Immune Tolerance; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D015847:Interleukin-4 / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D011817:Rabbits
chemical_list: D000961:Antilymphocyte Serum; D016207:Cytokines; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor