The Vibrio motor proteins, MotX and MotY, are associated with the basal body of Na-driven flagella and required for stator formation.
The four motor proteins PomA, PomB, MotX and MotY, which are believed to be stator proteins, are essential for motility by the Na(+)-driven flagella of Vibrio alginolyticus. When we purified the flagellar basal bodies, MotX and MotY were detected in the basal body, which is the supramolecular complex comprised of the rotor and the bushing, but PomA and PomB were not. By antibody labelling, MotX and MotY were detected around the LP ring. These results indicate that MotX and MotY associate with the basal body. The basal body had a new ring structure beneath the LP ring, which was named the T ring. This structure was changed or lost in the basal body from a DeltamotX or DeltamotY strain. The T ring probably comprises MotX and MotY. In the absence of MotX or MotY, we demonstrated that PomA and PomB were not localized to a cell pole. From the above results, we suggest that MotX and MotY of the T ring are involved in the incorporation and/or stabilization of the PomA/PomB complex in the motor.
Terashima
Hiroyuki
H
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-Ku, Nagoya 464-8602, Japan.
Fukuoka
Hajime
H
Yakushi
Toshiharu
T
Kojima
Seiji
S
Homma
Michio
M
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000502:physiology; D001426:Bacterial Proteins / Q000032:analysis* / Q000502:physiology; D005407:Flagella / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis* / Q000502:physiology; D020409:Molecular Motor Proteins / Q000032:analysis* / Q000502:physiology; D012964:Sodium / Q000378:metabolism; D015222:Sodium Channels / Q000378:metabolism / Q000502:physiology; D044149:Vibrio alginolyticus / Q000737:chemistry* / Q000166:cytology / Q000502:physiology
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008565:Membrane Proteins; D020409:Molecular Motor Proteins; C089633:MotX protein, bacteria; C088135:MotY protein, Vibrio; C107540:PomA protein, Vibrio; C107541:PomB protein, Vibrio; D015222:Sodium Channels; D012964:Sodium