Introduction of the anti-apoptotic baculovirus p35 gene in passion fruit induces herbicide tolerance, reduced bacterial lesions, but does not inhibits passion fruit woodiness disease progress induced by cowpea aphid-borne mosaic virus (CABMV).
The introduction of anti-apoptotic genes into plants leads to resistance to environmental stress and broad-spectrum disease resistance. The anti-apoptotic gene (p35) from a baculovirus was introduced into the genome of passion fruit plants by biobalistics. Eleven regenerated plants showed the presence of the p35 gene by PCR and/or dot blot hybridization. Transcriptional analysis of regenerated plants showed the presence of specific p35 transcripts in 9 of them. Regenerated plants containing the p35 gene were inoculated with the cowpea aphid-borne mosaic virus (CABMV), the bacterium Xanthomonas axonopodis pv passiflorae, and the herbicide, glufosinate, (Syngenta). None of the plants showed resistance to CABMV. Regenerated plants (p35+) showed less than half of local lesions showed by non-transgenic plants when inoculated with X. axonopodis and some p35+ plants showed increased tolerance to the glufosinate herbicide when compared to non-transgenic plants.
de Freitas
Daniele Scandiucci
DS
Departamento de Biologia Celular, Univesidade de Brasília, Brasília, Brasil.
Coelho
Marly C Felipe
MC
Souza
Manoel T
MT
Marques
Abi
A
Ribeiro
E Bergmann Morais
EB
D000818:Animals; D001042:Aphids / Q000821:virology; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D017798:Comovirus / Q000502:physiology*; D024861:Genetic Enhancement / Q000379:methods*; D053209:Herbicide Resistance / Q000502:physiology*; D029598:Passiflora / Q000382:microbiology / Q000502:physiology* / Q000821:virology*; D010935:Plant Diseases / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D051017:Apoptosis Regulatory Proteins; D014764:Viral Proteins; C492234:p35 protein, Baculovirus