Cell wall composition of smooth bromegrass plants selected for divergent fiber concentration.
Neutral detergent fiber (NDF) is considered the single best laboratory predictor of voluntary intake by ruminant livestock, creating interest in using NDF as a selection criterion in forage breeding programs. Because genetic reductions in NDF lead to increases in dry matter digestibility but not to changes in digestibility of the NDF fraction, we postulated that low-NDF plants do not have altered compositions of their cell walls. We tested this hypothesis using clones of smooth bromegrass (Bromus inermis Leyss.) with divergent NDF concentrations. High-NDF and low-NDF plants did not differ in cell wall concentrations or in the concentrations of any cell wall component (fucose, arabinose, rhamnose, galactose, glucose, xylose, mannose, uronic acids, and lignin). Instead, low-NDF plants had a cell wall that was more susceptible to solubilization in neutral detergent solution, suggesting that their cell walls were less well-developed as compared to high-NDF plants. NDF should not be used as a substitute for cell wall concentration in forage plants.
Casler
M D
MD
U.S. Dairy Forage Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1925 Linden Drive West, Madison, WI 53706-1108, USA. mdcasler@wisc.edu
Hatfield
R D
RD
D031724:Bromus / Q000737:chemistry / Q000648:ultrastructure*; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry*; D004043:Dietary Fiber / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry / Q000648:ultrastructure; D018547:Plant Stems / Q000737:chemistry / Q000648:ultrastructure
D002241:Carbohydrates; D004043:Dietary Fiber