title: Isolation and expansion of allogeneic myeloma-specific interferon-gamma producing T cells for adoptive immunotherapy.
abstract: Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4(+) and CD8(+) T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 x 106 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)-based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications.
authors.affiliation: Laboratory of Experimental Hematology and Cell Immunotherapy, Department of Clinical Hematology, University Hospital, Brno, Czech Republic. docadlikova@fnbrno.cz
authors.forename: Darina
authors.initials: D
authors.lastname: Ocadlikova
authors.forename: L
authors.initials: L
authors.lastname: Zahradova
authors.forename: L
authors.initials: L
authors.lastname: Kovarova
authors.forename: M
authors.initials: M
authors.lastname: Penka
authors.forename: J
authors.initials: J
authors.lastname: Zaloudik
authors.forename: T
authors.initials: T
authors.lastname: Buchler
authors.forename: R
authors.initials: R
authors.lastname: Hajek
authors.forename: J
authors.initials: J
authors.lastname: Michalek
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D005452:Fluoresceins / Q000737:chemistry; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007371:Interferon-gamma / Q000378:metabolism*; D008280:Magnetics; D008495:Medical Oncology / Q000379:methods; D009101:Multiple Myeloma / Q000276:immunology*; D013388:Succinimides / Q000737:chemistry; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D013388:Succinimides; D007371:Interferon-gamma