title: Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity.
abstract: Lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The activity of the pretreated lipase immobilized covalently on a silica gel was 530 U/g-matrix, which is 16 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Anam-dong, Sungbuk-ku, Seoul, Korea.
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Lee
authors.forename: Jung Mo
authors.initials: JM
authors.lastname: Kim
authors.forename: Seong Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Seung Wook
authors.initials: SW
authors.lastname: Kim
mesh_terms: D001665:Binding Sites; D001671:Biochemistry / Q000379:methods; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005227:Fatty Acids / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry; D008049:Lipase / Q000737:chemistry*; D008957:Models, Genetic; D012822:Silicon Dioxide / Q000737:chemistry; D013024:Soybean Oil / Q000378:metabolism*; D013237:Stereoisomerism; D013997:Time Factors
chemical_list: D004800:Enzymes, Immobilized; D005227:Fatty Acids; D012822:Silicon Dioxide; D013024:Soybean Oil; D008049:Lipase; D005990:Glycerol