title: Adsorption of human IgG on to poly(N-isopropylacrylamide)-based polymer particles.
abstract: Thermosensitive poly(N-isopropylacrylamide)-based polymer particles were synthesised, and screened for the adsorption of human immunoglobulin G (hIgG). At pH 9 the adsorption on microgel particles was strongly affected by temperature, approximately 40 mg hIgG/g support (90% of initial hIgG) being adsorbed at 40 degrees C but only 10% of initial hIgG at 25 degrees C. At pH 5 the maximum adsorbed amount (20 mg hIgG/g support) was similar for both temperatures. The adsorption of hIgG on to charged poly(methyl methacrylate)/poly(N-isopropylacrylamide) core-shell latexes was negligible (5-10 mg hIgG/g support) at the same temperature and pH conditions. The lower adsorption of hIgG onto the core-shell particles is explained by steric interactions due to the small size of the shell.
authors.affiliation: Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av Rovisco Pais, 1049-001, Lisbon, Portugal.
authors.forename: Claudia S O
authors.initials: CS
authors.lastname: Silva
authors.forename: Ricardo P
authors.initials: RP
authors.lastname: Baptista
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Santos
authors.forename: José M G
authors.initials: JM
authors.lastname: Martinho
authors.forename: Joaquim M S
authors.initials: JM
authors.lastname: Cabral
authors.forename: M Angela
authors.initials: MA
authors.lastname: Taipa
mesh_terms: D000178:Acrylamides / Q000493:pharmacokinetics*; D000180:Acrylic Resins; D000327:Adsorption; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry* / Q000493:pharmacokinetics; D013696:Temperature; D013997:Time Factors
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D007074:Immunoglobulin G; D011108:Polymers; C052970:poly-N-isopropylacrylamide