Analysis of biochemical reactions in equilibrium: hydrolysis of penicillin and ampicillin.
A general equilibrium model for a class of biochemical reactions was formulated, using the formalism of binding polynomials for the evaluations of the various species. Thermodynamic equilibrium constant was obtained from the observed equilibrium total concentrations of reactants at specified pH and certain ligand concentrations. Results for the hydrolysis of penicillin and ampicillin were analyzed with this equilibrium model. Predicted fractions of hydrolysis products at various pH values were calculated.
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Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.