title: Hepatocyte transplantation: State of the art.
abstract: Advances in biotechnology have allowed hepatocyte transplantation as a relevant proposition to treat liver disease. This procedure may change the crescent mortality in liver transplantation waiting lists due global organ shortage. Recent clinical trials have described promising results of hepatocyte transplantation for acute, acute-on-chronic and metabolic liver disease. In this report, we discuss progresses regarding hepatocyte culture, cryopreservation systems, hepatocyte immortalization, suitable recipient site for hepatocyte engraftment, cell differentiation and fusion into hepatocytes, current clinical trials, and summarize the bioartificial liver systems. These progressions motivate expectation concerning hepatocyte transplantation as a consistent therapy for liver disease.
authors.affiliation: Laboratório de Transplante Experimental da Disciplina de Transplante e Cirurgia do Fígado (LIM 37), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Flávio Henrique Ferreira
authors.initials: FH
authors.lastname: Galvão
authors.forename: Dahir Ramos
authors.initials: DR
authors.lastname: de Andrade Júnior
authors.forename: Dahir Ramos
authors.initials: DR
authors.lastname: de Andrade
authors.forename: Bruno Costa
authors.initials: BC
authors.lastname: Martins
authors.forename: Allan Garms
authors.initials: AG
authors.lastname: Marson
authors.forename: Christiano Vinicius
authors.initials: CV
authors.lastname: Bernard
authors.forename: Sânia Alves
authors.initials: SA
authors.lastname: Dos Santos
authors.forename: Telesforo
authors.initials: T
authors.lastname: Bacchella
authors.forename: Marcel Cerqueira César
authors.initials: MC
authors.lastname: Machado