The new approaches to preservation of graft cell integrity in preservation for transplantation.
Restoration of cell plasma membrane integrity after injury is essential for the survival of animal cells. In case of graft preservation or during chemotherapy in cancer, cell membrane integrity and the process of its repair are disrupted. Cytoprotective substances are important in such cases, as well as in other diseases, for example in myocardial infarction, acute insults and in chronic neurodegenerative diseases. Hyperosmolarity is a condition in which cell membrane stability may be damaged in vivo but preserved in the in vitro conditions. Hypertonicity causes water leaving from cells by osmosis, decreasing cell volume and increasing of intracellular ionic strength. High intracellular ionic strength perturbs cellular function by decreasing the rates of biochemical reaction. We review the new experimentally studied cytoprotective substances and their application in cell membrane protection. Moreover, we present our data on the effects of hyperosmolarity and its protective effect on cell internal structure.
Gewartowska
Magdalena
M
Department of Surgical Research and Transplantology, Medical Research Center, Polish Academy of Sciences, Warsaw, Poland.
Olszewski
Waldemar L
WL
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003566:Cytidine Diphosphate Choline / Q000494:pharmacology; D003681:Dehydration; D006035:Glycyrrhiza; D006360:Heat-Shock Proteins / Q000494:pharmacology; D006801:Humans; D006982:Hypertonic Solutions / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D020442:Poloxamer / Q000494:pharmacology; D011929:Razoxane / Q000494:pharmacology; D014021:Tissue Preservation / Q000379:methods*; D032070:Uncaria; D032341:Withania
D003451:Cryoprotective Agents; D006360:Heat-Shock Proteins; D006982:Hypertonic Solutions; D010936:Plant Extracts; D020442:Poloxamer; D003566:Cytidine Diphosphate Choline; D011929:Razoxane