Mass spectrometric investigation of buspirone drug in comparison with thermal analyses and MO-calculations.
The buspirone drug is usually present as hydrochloride form of general formula C(21)H(31)N(5)O(2).HCl, and of molecular weight (MW)=421.96. It is an analgesic anxiolytic drug, which does not cause sedative or depression of central nervous system. In the present work it is investigated using electron impact mass spectral (EI-MS) fragmentation at 70 eV, in comparison with thermal analyses (TA) measurements (TG/DTG and DTA) and molecular orbital calculation (MOC). Semi-empirical MO calculation, PM3 procedure, has been carried out on buspirone both as neutral molecule (in TA) and the corresponding positively charged species (in MS). The calculated MOC parameters include bond length, bond order, particle charge distribution on different atoms and heats of formation. The fragmentation pathways of buspirone in EI-MS lead to the formation of important primary and secondary fragment ions. The mechanism of formation of some important daughter ions can be illuminated from comparing with that obtained using electrospray ESIMS/MS mode mass spectrometer through the accurate mass measurement determination. The losses of the intermediate aliphatic part (CH2)4 due to cleavage of N-C bond from both sides is the primary cleavage in both techniques (MS and TA). The PM3 provides a base for fine distinction among sites of initial bond cleavage and subsequent fragmentation of drug molecule in both TA and MS techniques; consequently the choice of the correct pathway of such fragmentation knowing this structural session of bonds can be used to decide the active sites of this drug responsible for its chemical, biological and medical reactivity.
Zayed
M A
MA
Chemistry Department, Faculty of Education of Girls, El-Qaseem, Boridah, Saudi Arabia. mazayed429@yahoo.com
Fahmey
M A
MA
Hawash
M A
MA
El-Habeeb
Abeer A
AA
D014151:Anti-Anxiety Agents / Q000737:chemistry*; D002065:Buspirone / Q000737:chemistry*; D006358:Hot Temperature; D008432:Mathematical Computing; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization
D014151:Anti-Anxiety Agents; D002065:Buspirone