title: Swt1, a novel yeast protein, functions in transcription.
abstract: The conserved TREX complex couples transcription to nuclear mRNA export. Here, we report that the uncharacterized open reading frame YOR166c genetically interacts with TREX complex components and encodes a novel protein named Swt1 for "synthetically lethal with TREX." Co-immunoprecipitation experiments show that Swt1 also interacts with the TREX complex biochemically. Consistent with a potential role in transcription as suggested by its interaction with TREX, Swt1 localizes mainly to the nucleus. Importantly, deletion of Swt1 leads to decreased transcription. Taken together, these data suggest that Swt1 functions in gene expression in conjunction with the TREX complex.
authors.affiliation: Gene Center, Ludwig-Maximilians-University of Munich,Department of Chemistry and Biochemistry, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Röther
authors.forename: Emanuel
authors.initials: E
authors.lastname: Clausing
authors.forename: Anja
authors.initials: A
authors.lastname: Kieser
authors.forename: Katja
authors.initials: K
authors.lastname: Strässer
mesh_terms: D000595:Amino Acid Sequence; D004722:Endoribonucleases; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D004722:Endoribonucleases; C516564:Swt1 protein, S cerevisiae; D001616:beta-Galactosidase