Enzyme-responsive release of encapsulated proteins from biodegradable hollow capsules.
Biodegradable hollow capsules encapsulating proteins were prepared via layer-by-layer assembly of chitosan and dextran sulfate on protein-entrapping mesoporous silica particles and the subsequent removal of the silica. The enzymatic degradation of the capsules in the presence of chitosanase was explored by scanning electron microscopy (SEM). With increasing time, the chitosan component was degraded by chitosanase, and the capsules began to deform and were finally destroyed. Sustained release of the encapsulated proteins was attained by using the enzymatic degradation of the hollow capsules. The release behavior was successfully manipulated by altering the charge of capsule surface.
Itoh
Yuki
Y
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Matsusaki
Michiya
M
Kida
Toshiyuki
T
Akashi
Mitsuru
M
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002214:Capsules / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations; D016264:Dextran Sulfate / Q000737:chemistry*; D004337:Drug Carriers; D004339:Drug Compounding; D016503:Drug Delivery Systems; D004798:Enzymes / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012822:Silicon Dioxide / Q000737:chemistry*
D001672:Biocompatible Materials; D002214:Capsules; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004798:Enzymes; D012822:Silicon Dioxide; D048271:Chitosan; D016264:Dextran Sulfate; D006026:Glycoside Hydrolases; C019868:chitosanase