title: Limited diagnostic capacities of two commercial assays for the detection of Leptospira immunoglobulin M antibodies in Laos.
abstract: The diagnostic utility of immunochromatographic (Leptotek) and enzyme-linked immunosorbent assay (ELISA; Panbio) tests for the detection of Leptospira immunoglobulin M antibodies was assessed in febrile adults admitted in Vientiane, Laos. Both tests demonstrated poor diagnostic accuracy using admission serum (Leptotek sensitivity of 47.3% and specificity of 75.5%: ELISA sensitivity of 60.9% and specificity of 65.6%) compared to the Leptospira "gold standard" microscopic agglutination test.
authors.affiliation: Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR, and Centre for Clinical Vaccinology and Tropical Medicine, University of Oxford, UK. stuart@tropmedres.ac
authors.forename: Stuart D
authors.initials: SD
authors.lastname: Blacksell
authors.forename: Lee
authors.initials: L
authors.lastname: Smythe
authors.forename: Rattanaphone
authors.initials: R
authors.lastname: Phetsouvanh
authors.forename: Michael
authors.initials: M
authors.lastname: Dohnt
authors.forename: Rudy
authors.initials: R
authors.lastname: Hartskeerl
authors.forename: Meegan
authors.initials: M
authors.lastname: Symonds
authors.forename: Andrew
authors.initials: A
authors.lastname: Slack
authors.forename: Manivanh
authors.initials: M
authors.lastname: Vongsouvath
authors.forename: Viengmone
authors.initials: V
authors.lastname: Davong
authors.forename: Olay
authors.initials: O
authors.lastname: Lattana
authors.forename: Simmaly
authors.initials: S
authors.lastname: Phongmany
authors.forename: Valy
authors.initials: V
authors.lastname: Keolouangkot
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: White
authors.forename: Nicholas P J
authors.initials: NP
authors.lastname: Day
authors.forename: Paul N
authors.initials: PN
authors.lastname: Newton
mesh_terms: D002845:Chromatography / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis* / Q000097:blood; D007812:Laos; D007919:Leptospira / Q000276:immunology*; D007922:Leptospirosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D012680:Sensitivity and Specificity
chemical_list: D007075:Immunoglobulin M