ESE-3 transcription factor is involved in the expression of death receptor (DR)-5 through putative Ets sites.
The death receptor 5 (DR-5), a receptor for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), is critical for TRAIL-mediated apoptosis in various tumor cells. The ESE-3, a member of Ets transcription factors, regulates the expression of a variety of cellular genes by binding to purine-rich GGAA/T core sequence in cooperation with other transcription factors and co-factors. In this study, we demonstrate for the first time that ESE-3 regulates DR-5 expression through Ets binding sequences on the DR-5 promoter. Using a combination of the electrophoretic mobility shift assay and the luciferase reporter assay, we identified putative Ets sites responsible for ESE-3 transcriptional activity on the DR-5 promoter. In addition, we show the possible involvement of co-factors CBP and p300 in ESE-3-mediated DR-5 up-regulation.
Lim
Jun Hee
JH
Department of Immunology, School of Medicine, Keimyung University, 194 DongSan-Dong, Taegu 700-712, Republic of Korea.
Cho
Je-Yoel
JY
Park
Yong Bok
YB
Park
Jong-Wook
JW
Kwon
Taeg Kyu
TK
D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D050783:Proto-Oncogene Proteins c-ets / Q000235:genetics / Q000378:metabolism*; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
C403306:EHF protein, human; D050783:Proto-Oncogene Proteins c-ets; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D014157:Transcription Factors