title: Glycated polyelectrolyte multilayer films: differential adhesion of primary versus tumor cells.
abstract: Glycated polymers have already been widely employed for cell transfection studies, as cells possess specific lectins. However, up to now, these glycated polymers have barely been investigated for their cell adhesive properties, save macrophages. In this work, we use polyelectrolyte multilayer films made of poly(L-lysine) and poly(L-glutamic) acid as polymeric substrates to investigate the role of sugar molecules (e.g., mannose and lactose) on the adhesion of primary cells as compared to that of a tumor cell line. The glycated polymeric films were compared to ungrafted and chemically cross-linked films, which are known to present opposite adhesive properties. A differential adhesion could be evidenced on mannose grafted films: primary chondrocytes adhere and proliferate well on these films, whereas chondrosarcoma cells do not grow well. Although present, the effect of lactose on cell adhesion was much less important. This adhesion, mediated by glycated polymers, appears to be specific. These results show that it is possible to use glycated polyelectrolytes not only as nonviral vectors but also as cell adhesive substrates.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 595, Faculté de Chirurgie Dentaire, Université Louis Pasteur, 11 rue Humann, 67085 Strasbourg Cedex, France.
authors.forename: Aurore
authors.initials: A
authors.lastname: Schneider
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Bolcato-Bellemin
authors.forename: Gregory
authors.initials: G
authors.lastname: Francius
authors.forename: Justyna
authors.initials: J
authors.lastname: Jedrzejwska
authors.forename: Pierre
authors.initials: P
authors.lastname: Schaaf
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Voegel
authors.forename: Benoit
authors.initials: B
authors.lastname: Frisch
authors.forename: Catherine
authors.initials: C
authors.lastname: Picart
mesh_terms: D000268:Adhesiveness; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D045744:Cell Line, Tumor; D019902:Chondrocytes / Q000378:metabolism; D004573:Electrolytes / Q000737:chemistry*; D006801:Humans; D008358:Mannose / Q000737:chemistry; D018625:Microscopy, Atomic Force; D009369:Neoplasms / Q000378:metabolism*; D011099:Polyglutamic Acid / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001672:Biocompatible Materials; D004573:Electrolytes; D011107:Polylysine; D011099:Polyglutamic Acid; D008358:Mannose