M13 bacteriophage coat proteins engineered for improved phage display.
This chapter describes a method for increasing levels of protein fusions displayed on the surfaces of M13 bacteriophage particles. By introducing mutations into the anchoring M13 coat protein, protein display levels can be increased by up to two orders of magnitude. Experimental methods are presented for the design, construction, and screening of phage-displayed libraries for improved protein display.
Sidhu
Sachdev S
SS
Department of Protein Engineering, Genentech Inc., South San Francisco, CA, USA.
Feld
Birte K
BK
Weiss
Gregory A
GA
D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D019151:Peptide Library; D015202:Protein Engineering
D036022:Capsid Proteins; D019151:Peptide Library; C025668:coat protein, Bacteriophage M13