Genotypic characterisation of Indian cattle, buffalo and sheep isolates of Echinococcus granulosus.
Twelve isolates of Echinococcus granulosus, collected from domestic animals, including cattle, buffalo and sheep were analysed for DNA nucleotide sequence variation within mitochondrial cytochrome c oxidase I (coxI), NADH dehydrogenase subunit I (nadI) and internal transcribed spacer gene I (ITS1). After analysis of sequence information this was found that the fragment size of ITS1 of buffalo isolate was more in comparison to cattle and sheep isolates. Based on the nadI genotype this was found that Indian cattle, buffalo and sheep isolates could be grouped into E. granulosus sensu stricto. Based on coxI genotype two sheep isolates and one buffalo isolate were homologous to G2 genotype. Rests of the isolates were microvariants of G2 genotype. Presence of G2 genotype in buffalo is the first report of this genotype from this host.
Bhattacharya
D
D
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata 700037, India. debasis63@rediffmail.com
Bera
A K
AK
Bera
B C
BC
Maity
A
A
Das
S K
SK
D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D003575:Cytochromes c1 / Q000235:genetics; D018509:DNA, Helminth / Q000032:analysis / Q000737:chemistry*; D021901:DNA, Intergenic / Q000235:genetics; D004443:Echinococcosis / Q000469:parasitology / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005819:Genetic Markers; D005838:Genotype; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D017385:Sequence Homology; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*
D018509:DNA, Helminth; D021901:DNA, Intergenic; D005819:Genetic Markers; D003575:Cytochromes c1; D009245:NADH Dehydrogenase