Monitoring of stool microbiota in subjects with diarrhea indicates distortions in composition.
We utilized denaturing gradient gel electrophoresis profiling to survey stool microbiota in 175 persons with diarrhea and 113 asymptomatic persons in a diarrhea surveillance study. Compared with healthy controls, the microbiota profiles in diarrhea cases more frequently exhibited decreased diversity and strong bands indicating the overgrowth of selected bacteria or bacterial groups.
Mai
Volker
V
Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Rm. 934-B MSTF, 10 S. Pine St., Baltimore, MD 21201, USA. vmai@epi.umaryland.edu
Braden
Christopher R
CR
Heckendorf
Jill
J
Pironis
Baiba
B
Hirshon
Jon Mark
JM
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S