The presumptive floor plate (notoplate) induces behaviors associated with convergent extension in medial but not lateral neural plate cells of Xenopus.
In previous work (Elul, T., Keller, R., 2000. Monopolar protrusive activity: a new morphogenic cell behavior in the neural plate dependent on vertical interactions with the mesoderm in Xenopus. Dev. Biol. 224, 3-19; Ezin, A.M., Skoglund, P. Keller, R. 2003. The midline (notochord and notoplate) patterns the cell motility underlying convergence and extension of the Xenopus neural plate. Dev. Biol. 256, 100-114), the midline tissues of notochord and overlying notoplate were found to induce the monopolar, medially directed protrusive activity of deep neural cells. This behavior is thought to drive the mediolateral intercalation and convergent extension of the neural plate in Xenopus. Here we address the issue of whether the notochord, the notoplate, or both is essential for this induction. Our strategy was to remove the notochord, leaving the overlying notoplate intact, and determine whether it alone can induce the monopolar, medially directed cell behavior. We first establish that the notoplate (presumptive floor plate), when separated from the underlying notochord in the early neurula (stages 13-14), will independently mature into a floor plate as assayed three criteria: (1) continued expression of an early marker, sonic hedgehog, and a later, marker, F-spondin; (2) the display of the notoplate/floor plate-specific randomly oriented protrusive activity; (3) the characteristic lack of mixing of cells between the notoplate and lateral neural plate. Under these conditions, in the presence of a mature notoplate/floor plate and in the absence of the notochord, the characteristic monopolar, medially directed behavior occurred, but only locally near the midline. These results show that the notoplate/floor plate capacity to induce the medially directed motility is limited in range, and they suggest that the notochord is necessary for the normally observed longer range induction in lateral neural plate cells. This work helps to further the understanding of molecular and tissue interactions required for convergent extension.
Ezin
Akouavi M
AM
Department of Biology, Gilmer Hall, University of Virginia, Charlottesville, VA 22903, USA. max@caltech.edu
Skoglund
Paul
P
Keller
Ray
R
D000818:Animals; D030801:Animals, Genetically Modified; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D002490:Central Nervous System / Q000196:embryology*; D004627:Embryonic Induction / Q000502:physiology*; D053823:Hedgehog Proteins / Q000096:biosynthesis / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides / Q000235:genetics; D029867:Xenopus Proteins / Q000096:biosynthesis / Q000235:genetics; D014982:Xenopus laevis
D053823:Hedgehog Proteins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D029867:Xenopus Proteins; C498958:spon1 protein, Xenopus