title: A disposable, screen-printed electrode for the amperometric determination of azide based on the immobilization with catalase or tyrosinase.
abstract: A disposable, screen-printed electrode based on the immobilization of catalase or tyrosinase was developed to construct biosensors for the amperometric determination of azide. The determination principles for azide by these two methods are based on inhibiting the enzymatic consumption of an electrode-detectable substance (hydrogen peroxide or catechol) on an enzyme-immobilized electrode. Both of these methods show a sensitive detection range and a short measuring time.
authors.affiliation: Department of Chemical Engineering, Hong Kong University of Science and Technology, Kowloon, Hong Kong. kecuiyue@ust.hk
authors.forename: Yue
authors.initials: Y
authors.lastname: Cui
authors.forename: John P
authors.initials: JP
authors.lastname: Barford
authors.forename: Reinhard
authors.initials: R
authors.lastname: Renneberg
mesh_terms: D001386:Azides / Q000737:chemistry*; D015374:Biosensing Techniques; D002374:Catalase / Q000737:chemistry*; D004156:Catechol Oxidase / Q000737:chemistry; D002396:Catechols / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D004798:Enzymes / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D013997:Time Factors
chemical_list: D001386:Azides; D002396:Catechols; D004798:Enzymes; D004800:Enzymes, Immobilized; D006861:Hydrogen Peroxide; D004156:Catechol Oxidase; D002374:Catalase; D014442:Monophenol Monooxygenase; C034221:catechol