How to map ses, a mutant of Arabidopsis thaliana affecting pollen development.
In Arabidopsis, map-based cloning has been developed to an effective method in mutant genetic analysis because high-density markers are available, candidate genes or genomic sequences can be amplified by PCR and transgenic techniques are simplified. Mutant ses named from shortened early-stage siliques was used as an example to show how to map a mutant in this day. By the process of bulked segregants analysis, linkage testing, large-scale and fine scale mapping, mutant ses was narrowed into a 67 kb interval from CER448792 (2000541 bp) to CER464544 (2067844 bp) crossing over the right of BAC F12K11 to the left of the BAC F4H5 including at most 22 putative genes on the top of chromosome l. In sequence-based map of Arabidopsis genes with Mutant phenotype (SMAGMP) mutant ses was between ATlg06150 (EMB1444) and ATlg08060 (MOM). The SES mapping also showed that developed markers on polymorphism site of CAPC not only were simplified and but worked well. 24 markers from CAPC used in the mapping maybe help Arabidopsis researches with others and the methods related to SES mapping also gave an example of positional cloning.
Liu
T L
TL
State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, China.
Kuai
B K
BK
Liu
Z X
ZX
Zhao
D L
DL
Shen
D L
DL
D017360:Arabidopsis / Q000235:genetics*; D032461:Chromosomes, Plant / Q000235:genetics*; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis / Q000379:methods*; D020161:Physical Chromosome Mapping / Q000379:methods*; D011058:Pollen / Q000235:genetics / Q000254:growth & development*