title: A rapid HPLC/ESI-MS method for the quantitative determination of oridonin in rat plasma.
abstract: A rapid and accurate method using liquid chromatography with electrospray ionization mass spectrometric detection (HPLC/ESI-MS) was developed and validated for the determination of oridonin in rat plasma. The analytes were extracted with ethyl acetate-n-butyl alcohol (100:2, v/v) after spiking the samples with ethyl hydroxybenzoate (internal standard). The separation was carried out on a Diamon-sil C18 column with an isocratic mobile phase consisting of methanol-water (80:20, v/v) at a flow rate of 1.0 ml/min. The lower limit of quantification (LLOQ) of the method was 10 ng/ml and the linear range was 10-4000 ng/ml. The intra-day and inter-day accuracy and precision of the assay were less than 9%. This method has been applied successfully to a preliminary pharmacokinetic study involving the intravenous administration of oridonin to rats.
authors.affiliation: Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
authors.forename: Jin
authors.initials: J
authors.lastname: Sun
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.forename: Jingling
authors.initials: J
authors.lastname: Tang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.forename: Jinling
authors.initials: J
authors.lastname: Fang
authors.forename: Dawei
authors.initials: D
authors.lastname: Chen
authors.forename: Zhonggui
authors.initials: Z
authors.lastname: He
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000097:blood* / Q000493:pharmacokinetics; D045786:Diterpenes, Kaurane; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D019999:Pharmaceutical Solutions; D011786:Quality Control; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D045786:Diterpenes, Kaurane; D019999:Pharmaceutical Solutions; C011959:oridonin