Histological evaluation of the osteoinduction capability of human dentine.
Aim To assess whether human dentine has the potential to promote the development of calcified tissues when implanted in the muscle tissue of mice. Methodology Root canals in extracted human teeth were instrumented to produce dentine fragments. The dentine fragments produced were divided into two. In group 1, fragments were demineralized and sterilized. In group 2, the fragments were not submitted to any additional treatment. The dentine fragments were then implanted in the muscle of mice. In group 3, the muscles were implanted with rehydrated lyophilized human bone powder. Animals were killed following test periods of 7, 15, 30, 60, 120 and 180 days, the fragments were removed together with adjacent muscle and examined under light microscopy to assess calcification. Results Areas of calcification were observed in groups 1 and 3 after a period of 180 days. In group 2, the surrounding tissues displayed only chronic inflammatory infiltration. Conclusions On the basis of the experimental model adopted in this study, fibroblast-rich connective tissue formed in groups 1 and 3, which could reflect an osteoinductive process. Further studies are suggested to identify which dentinal factors are capable of inducing the formation of a calcified matrix.
Machado
M E L
ME
Discipline of Endodontics, School of Dentistry, Camilo Castelo Branco University, São Paulo, Brazil. professormachado@hotmail.com
Souza
A S
AS
Araújo
V C
VC
D000818:Animals; D016025:Bone Transplantation; D003238:Connective Tissue / Q000473:pathology; D003648:Decalcification Technique; D003804:Dentin / Q000502:physiology*; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008297:Male; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000473:pathology; D018482:Muscle, Skeletal / Q000473:pathology*; D009999:Ossification, Heterotopic / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology*; D013242:Sterilization; D013997:Time Factors; D016042:Transplantation, Heterotopic