Inhibition of PC-PLC blocked the survival of mouse neural cells by up-regulating the expression of integrin beta4 and Rb.
In order to understand the survival signals of neural cells during the development of the central nervous system (CNS), we explored the role of phosphatidylcholine-specific phospholipase C (PC-PLC) in the survival of primary culture mouse neural cells (MNCs). We found that when the activity of PC-PLC in the neural cells was suppressed by its specific inhibitor, D609 (tricyclodecan-9-yl-xanthogenate), the cell survival was blocked, the viability of the cells remarkably declined. At the same time, the expressions of integrin beta4 and Rb protein were evidently elevated. We also examined the intracellular ROS (reactive oxygen species) levels after the cells were treated with D609. The result showed that the ROS level in neural cells was reduced. Our data suggested that PC-PLC had an important role in regulating neural cell survival, and PC-PLC might perform its function by upregulating the expressions of integrin beta4 and Rb protein. The changes of intracellular ROS level induced by D609 indicated that a modest level of ROS might be indispensable to neural cell survival.
Lv
Xin
X
Institute of Developmental Biology, School of Life Science, Shandong University, Jinan, China.
Wang
Nan
N
Su
Le
L
Zhang
Shangli
S
Miao
Junying
J
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D001952:Bridged-Ring Compounds / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D039663:Integrin beta4 / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009636:Norbornanes; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D016160:Retinoblastoma Protein / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism; D013859:Thiocarbamates; D013871:Thiones / Q000494:pharmacology; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D001952:Bridged-Ring Compounds; D004791:Enzyme Inhibitors; D039663:Integrin beta4; D009636:Norbornanes; D017382:Reactive Oxygen Species; D016160:Retinoblastoma Protein; D013859:Thiocarbamates; D013871:Thiones; C046498:tricyclodecane-9-yl-xanthogenate; D010738:Type C Phospholipases; C105598:phosphatidylcholine-specific phospholipase C