Kinesin-13s form rings around microtubules.
Kinesin is a superfamily of motor proteins that uses the energy of adenosine triphosphate hydrolysis to move and generate force along microtubules. A notable exception to this general description is found in the kinesin-13 family that actively depolymerizes microtubules rather than actively moving along them. This depolymerization activity is important in mitosis during chromosome segregation. It is still not fully clear by which mechanism kinesin-13s depolymerize microtubules. To address this issue, we used electron microscopy to investigate the interaction of kinesin-13s with microtubules. Surprisingly, we found that proteins of the kinesin-13 family form rings and spirals around microtubules. This is the first report of this type of oligomeric structure for any kinesin protein. These rings may allow kinesin-13s to stay at the ends of microtubules during depolymerization.
Tan
Dongyan
D
Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Asenjo
Ana B
AB
Mennella
Vito
V
Sharp
David J
DJ
Sosa
Hernando
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D006224:Cricetinae; D004330:Drosophila / Q000378:metabolism; D029721:Drosophila Proteins / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D016547:Kinesins / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure*; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D008958:Models, Molecular; D008968:Molecular Conformation; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure
D029721:Drosophila Proteins; D014404:Tubulin; C490417:mitotic centromere-associated kinesin, hamster; D000255:Adenosine Triphosphate; C481339:KLP10A protein, Drosophila; C481338:Klp59C protein, Drosophila; D016547:Kinesins