Compositional assessment of event DAS-59122-7 maize using substantial equivalence.
Event DAS-59122-7 (Herculex RW) maize (Zea mays L.) plants were transformed to express the Cry34Ab1 and Cry35Ab1 binary insecticidal crystal proteins originally isolated from Bacillus thuringiensis Berliner (Bt) strain PS149B1. These proteins protect maize roots from attack by corn rootworms, Diabrotica spp. DAS-59122-7 maize also contains the pat gene, originally isolated from Streptomyces viridochromogenes, which confers tolerance to glufosinate-ammonium herbicides (e.g. Liberty). We assessed the composition of these transgenic plants (with and without Liberty herbicide treatment), grown at a total of eight fields sites over 2 years, by applying the principle of substantial equivalence. Forage and grain samples were analyzed for proximates, fiber and minerals, and grain was further analyzed for amino acids, fatty acids, vitamins, secondary metabolites and anti-nutrients. Data plots were prepared that allow for efficient investigation of equivalency between event DAS-59122-7 maize and a non-transgenic near-isogenic maize line grown contemporaneously. Results demonstrated that DAS-59122-7 maize is equivalent to non-transgenic maize with respect to these important constituents.
Herman
Rod A
RA
Dow AgroSciences LLC, Indianapolis, IN 46268, USA. raherman@dow.com
Storer
Nicholas P
NP
Phillips
Amy M
AM
Prochaska
Lee M
LM
Windels
Pieter
P
D000596:Amino Acids / Q000032:analysis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001427:Bacterial Toxins / Q000737:chemistry* / Q000235:genetics; D004731:Endotoxins / Q000737:chemistry* / Q000235:genetics; D030841:Food, Genetically Modified; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000235:genetics; D008903:Minerals / Q000032:analysis; D010572:Pest Control, Biological / Q000379:methods; D030821:Plants, Genetically Modified / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry* / Q000235:genetics
D000596:Amino Acids; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D008903:Minerals; C030956:insecticidal crystal protein, Bacillus Thuringiensis