Determination of chloramphenicol in royal jelly by liquid chromatography/tandem mass spectrometry.
A liquid chromatographic/tandem mass spectrometric method was developed and validated for the determination of chloramphenicol (CAP) in royal jelly. Royal jelly samples were first denatured with lead acetate solution, and the CAP was extracted with solid-phase extraction before separation by liquid chromatography. A triple-quadrupole mass spectrometer operated in the negative electrospray ionization and selected-reaction monitoring mode was used for the detection of CAP. For method validation, royal jelly samples were fortified at CAP levels between 0.1 and 10.0 microg/kg; at these levels, recovery values (internal standard-corrected) ranged from 93.3 to 105.0%, and the within-laboratory reproducibility (relative standard deviation) was < or = 9.1%. The decision limit was 0.07 microg/kg, and the detection capability was 0.1 microg/kg.
Jiang
Yuan
Y
Jiangsu Entry-Exit Inspection and Quarantine Bureau, APFIC, Food Laboratory, 99 Zhonghua Rd, Nanjing, People's Republic of China.
Xu
Jin-Zhong
JZ
Shen
Chong-Yu
CY
Ding
Tao
T
Chen
Hui-Lan
HL
Wu
Bin
B
Shen
Wei-Jian
WJ
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000592:standards; D002701:Chloramphenicol / Q000032:analysis* / Q000592:standards; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005227:Fatty Acids / Q000032:analysis* / Q000592:standards; D005504:Food Analysis / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005506:Food Contamination / Q000032:analysis* / Q000706:statistics & numerical data; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D000099219:Royal Jelly
D000900:Anti-Bacterial Agents; D002701:Chloramphenicol; D005227:Fatty Acids; D000099219:Royal Jelly