title: [Changes of mitochondrial membrane potential (delta psi m) and calcium concentration in cultured glomerular mesangial cells of rats with obstructive jaundice].
abstract: OBJECTIVE
  To explore the effect of mitochondrial membrane potential (delta psi m) and calcium concentration on the kidney injury of rats with obstructive jaundice and to probe into the underlying mechanism.
  
  
  METHODS
  Sixty male Sprague-Dowley rats, weighing 200 g to 250 g were randomly divided into three groups: control group; sham operation group; experiment group. And the rats of experiment group were subdivided into two groups according to the time (15 d, 21 d) after the common bile duct ligation. The experiment groups were rendered jaundiced by doubly ligating the common bile duct. Glomeruli were isolated, the glomerular mesangial cells (GMCs) were cultured. The mitochondrial membrane potential (delta psi m) was measured by flow cytometry; the concentration of calcium was measured by confocal microscopes.
  
  
  RESULTS
  The amount of mitochondrial membrane potential (delta psi m) was decreased (P < 0.05) in the rats with obstructive jaundice, compared with the control; the concentration of calcium was increased (P < 0.05) in the rats with obstructive jaundice, compared with the control. Conclusion The calcium incurrent that leads to changes in amount of mitochondrial membrane potential (delta psi m) has significant effect on the kidney injury of the rats with obstructive jaundice.
authors.affiliation: Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610061, China.
authors.forename: An-yi
authors.initials: AY
authors.lastname: Qiao
authors.forename: Yi-de
authors.initials: YD
authors.lastname: Qian
authors.forename: Xing
authors.initials: X
authors.lastname: Yang
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.forename: Jie-shou
authors.initials: JS
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Peng
mesh_terms: D000818:Animals; D041781:Jaundice, Obstructive / Q000503:physiopathology*; D008297:Male; D008564:Membrane Potentials; D050527:Mesangial Cells / Q000502:physiology*; D051336:Mitochondrial Membranes / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley