SBD.4 stimulates regenerative processes in vitro, and wound healing in genetically diabetic mice and in human skin/severe-combined immunodeficiency mouse chimera.
In search of novel angiostimulators, we performed a high-throughput screening of medicinal plants beneficial for blood circulation. From the panel of positive hits, Angelica sinensis was selected for further investigation. Purified down to a low-molecular-weight fraction and characterized by high-performance liquid chromatography-mass spectrometry, the material, named SBD.4A, revealed a particularly strong wound healing activity in the diabetic mouse wound-healing model, and in the human/severe combined immunodeficiency mouse chimera wound-healing model. In both models, SBD.4A compared favorably with the Food and Drug Administration-approved wound-healing drug becaplermin, suggesting that this botanical product could be a valuable treatment for difficult-to-heal wounds. Further high-performance liquid chromatography fractionation of SBD.4A yielded a hydrophilic fraction, which strongly stimulated endothelial cell proliferation, tridimensional endothelial cell network formation, as well as the proliferation of human dermal fibroblasts and type I collagen secretion. Because angiogenesis and fibroblast proliferation are essential for wound healing, we propose that this liquid chromatography-mass spectrometry-defined hydrophilic fraction is at least partially responsible for the wound-healing activity of SBD.4A.
Zhao
Hui
H
Sunny BioDiscovery, Santa Clara, California 95050, USA.
Mortezaei
Reza
R
Wang
Yanrong
Y
Sheng
Xinsheng
X
Aria
Fariba
F
Bojanowski
Krzysztof
K
D029971:Angelica sinensis; D000818:Animals; D000077214:Becaplermin; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002678:Chimera; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D016513:Mice, SCID; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D008517:Phytotherapy; D018517:Plant Roots; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D020574:Proto-Oncogene Proteins c-sis; D012867:Skin / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; C516837:SBD.4A herbal preparation; D000077214:Becaplermin; D003094:Collagen