Free radical scavenging effect of Pu-erh tea extracts and their protective effect on oxidative damage in human fibroblast cells.
In the present study, we successively extracted the Pu-erh tea with acetone, water, chloroform, ethyl acetate, and n-butanol, and the extracts were then isolated by column chromatography. Our study demonstrates that the Pu-erh tea ethyl acetate extract, n-butanol extract, and their fractions had superoxide anion and hydroxyl radical scavenging activity: fractions 2 and 8 from the ethyl acetate extract and fractions 2, 4, and 5 from the n-butanol extract showed protective effects against hydrogen peroxide-induced damage in human fibroblast HPF-1 cells and increased the cells' viability under normal cell culture conditions. In addition, it is found that these fractions, except fraction 5 from the n-butanol extract, decreased the accumulation of intracellular reactive oxygen species in hydrogen peroxide-induced HPF-1 cells. Interestingly, the antioxidant effect of fraction 8 from the ethyl acetate extract on the above four systems was much stronger than that of the typical green tea catechin (-)-epigallocatechin-3-gallate, but there were almost no monomeric polyphenols, theaflavins, and gallic acid in fraction 8.
Jie
Guoliang
G
Zhejiang University Tea Research Institute, 268 Kaixuan Road, Hangzhou 310029, People's Republic of China.
Lin
Zhi
Z
Zhang
Longze
L
Lv
Haipeng
H
He
Puming
P
Zhao
Baolu
B
D002460:Cell Line; D004622:Embryo, Mammalian; D005285:Fermentation; D005347:Fibroblasts / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D013481:Superoxides / Q000037:antagonists & inhibitors; D013662:Tea / Q000737:chemistry*
D016166:Free Radical Scavengers; D010936:Plant Extracts; D013662:Tea; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide