title: Natural presence of Campylobacter spp. in various internal organs of commercial broiler breeder hens.
abstract: Campylobacter are known to cause acute bacterial gastroenteritis in humans. Poultry products have been implicated as a significant source of these infections. Six experiments were performed to determine whether Campylobacter could be isolated naturally from the primary and secondary lymphoid organs, liver/gallbladder, and ceca of commercial broiler breeder hens. Broiler breeder hens were acquired from different commercial sources during the early, middle, and late lay cycles. The birds were euthanatized, defeathered, and aseptically opened. To reduce the possibility of cross-contamination between samples, the thymus, spleen, and liver/gallbladder were aseptically removed prior to removal of the ceca. Individual samples were placed in sterile bags, packed on ice, and transported to the laboratory for evaluation. In this study Campylobacter were found in 11 of 43 thymii, eight of 43 spleens, four of 43 liver/gallbladders, and 30 of 43 ceca. Overall, 28 of 53 isolates from the above samples were Campylobacter coli and 25 of 53 isolates were found to be Campylobacter jejuni.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Poultry Microbiological Safety Research Unit, Russell Research Center, Athens, GA 30605, USA.
authors.forename: N A
authors.initials: NA
authors.lastname: Cox
authors.forename: L J
authors.initials: LJ
authors.lastname: Richardson
authors.forename: R J
authors.initials: RJ
authors.lastname: Buhr
authors.forename: P J
authors.initials: PJ
authors.lastname: Fedorka-Cray
authors.forename: J S
authors.initials: JS
authors.lastname: Bailey
authors.forename: J L
authors.initials: JL
authors.lastname: Wilson
authors.forename: K L
authors.initials: KL
authors.lastname: Hiett
mesh_terms: D000818:Animals; D002167:Campylobacter / Q000302:isolation & purification*; D002432:Cecum / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D005260:Female; D005704:Gallbladder / Q000382:microbiology*; D008099:Liver / Q000382:microbiology*; D010058:Oviposition; D013154:Spleen / Q000382:microbiology*; D013950:Thymus Gland / Q000382:microbiology*