title: Generation of a novel fluorescent product, monochlorofluorescein from dichlorofluorescin by photo-irradiationdagger.
abstract: Dichlorofluorescin (DCFH), a widely used fluorescent probe for reactive oxygen species (ROS) was decomposed completely and generated two distinct fluorescent products by photo-irradiation at 254 nm for 30 min. In the previous study, we had shown that one was dichlorofluorescein (DCF), a well known oxidized product of DCFH. In this study we investigated the other product and identified it as monochlorofluorescein (MCF) by 1H-NMR and fast atom bombardment/mass spectrum (FAB/MS) analyses. MCF was generated by photo-irradiation, but not by ROS. On the other hand, DCF was produced by both photo-irradiation and ROS. MCF showed similar fluorescent emission spectrum to DCF, however, its fluorescence intensity was more than that of DCF. The kinetic study suggested that MCF was not generated from DCF but from monochlorofluorescin, which might be generated from DCFH by photo-irradiation.
authors.affiliation: Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Division of Applied Biological Sciences, 4-3-11 Takeda, Kofu, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Matsugo
authors.forename: Masaaki
authors.initials: M
authors.lastname: Sasai
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Shinmori
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Yasui
authors.forename: Minoru
authors.initials: M
authors.lastname: Takeuchi
authors.forename: Toru
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005452:Fluoresceins / Q000737:chemistry* / Q000528:radiation effects*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods; D014466:Ultraviolet Rays
chemical_list: D005452:Fluoresceins; C037631:2',7'-dichlorofluorescein