Study of biochemical pathways and enzymes involved in pyrene degradation by Mycobacterium sp. strain KMS.
Pyrene degradation is known in bacteria. In this study, Mycobacterium sp. strain KMS was used to study the metabolites produced during, and enzymes involved in, pyrene degradation. Several key metabolites, including pyrene-4,5-dione, cis-4,5-pyrene-dihydrodiol, phenanthrene-4,5-dicarboxylic acid, and 4-phenanthroic acid, were identified during pyrene degradation. Pyrene-4,5-dione, which accumulates as an end product in some gram-negative bacterial cultures, was further utilized and degraded by Mycobacterium sp. strain KMS. Enzymes involved in pyrene degradation by Mycobacterium sp. strain KMS were studied, using 2-D gel electrophoresis. The first protein in the catabolic pathway, aromatic-ring-hydroxylating dioxygenase, which oxidizes pyrene to cis-4,5-pyrene-dihydrodiol, was induced with the addition of pyrene and pyrene-4,5-dione to the cultures. The subcomponents of dioxygenase, including the alpha and beta subunits, 4Fe-4S ferredoxin, and the Rieske (2Fe-2S) region, were all induced. Other proteins responsible for further pyrene degradation, such as dihydrodiol dehydrogenase, oxidoreductase, and epoxide hydrolase, were also found to be significantly induced by the presence of pyrene and pyrene-4,5-dione. Several nonpathway-related proteins, including sterol-binding protein and cytochrome P450, were induced. A pyrene degradation pathway for Mycobacterium sp. strain KMS was proposed and confirmed by proteomic study by identifying almost all the enzymes required during the initial steps of pyrene degradation.
Liang
Yanna
Y
Department of Civil and Environmental Engineering, 4105 Old Main Hill, Utah State University, Logan, UT 84322-4105, USA.
Gardner
Dale R
DR
Miller
Charles D
CD
Chen
Dong
D
Anderson
Anne J
AJ
Weimer
Bart C
BC
Sims
Ronald C
RC
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D013058:Mass Spectrometry; D009161:Mycobacterium / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000494:pharmacology; D020543:Proteome; D011721:Pyrenes / Q000378:metabolism*
D001426:Bacterial Proteins; D011084:Polycyclic Aromatic Hydrocarbons; D020543:Proteome; D011721:Pyrenes; C030984:pyrene