High shikimate production from quinate with two enzymatic systems of acetic acid bacteria.
3-Dehydroshikimate was formed with a yield of 57-77% from quinate via 3-dehydroquinate by two successive enzyme reactions, quinoprotein quinate dehydrogenase (QDH) and 3-dehydroquinate dehydratase, in the cytoplasmic membranes of acetic acid bacteria. 3-Dehydroshikimate was then reduced to shikimate (SKA) with NADP-dependent SKA dehydrogenase (SKDH) from the same organism. When SKDH was coupled with NADP-dependent D-glucose dehydrogenase (GDH) in the presence of excess D-glucose as an NADPH re-generating system, SKDH continued to produce SKA until 3-dehydroshikimate added initially in the reaction mixture was completely converted to SKA. Based on the data presented, a strategy for high SKA production was proposed.
Adachi
Osao
O
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan. osao@yamaguchi-u.ac.jp
Ano
Yoshitaka
Y
Toyama
Hirohide
H
Matsushita
Kazunobu
K
D016947:Acetobacteraceae / Q000201:enzymology; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D053898:Biosynthetic Pathways; D020600:Gluconobacter oxydans / Q000201:enzymology*; D005947:Glucose / Q000378:metabolism; D005948:Glucose Dehydrogenases / Q000378:metabolism; D006836:Hydro-Lyases / Q000378:metabolism; D011801:Quinic Acid / Q000378:metabolism*; D012765:Shikimic Acid / Q000031:analogs & derivatives / Q000378:metabolism*
D011801:Quinic Acid; D012765:Shikimic Acid; D000429:Alcohol Oxidoreductases; D005948:Glucose Dehydrogenases; C022641:Shikimate dehydrogenase; C033955:glucose dehydrogenase (pyrroloquinoline-quinone); D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase; D005947:Glucose