Detection of S-nitrosothiols in biological fluids: a comparison among the most widely applied methodologies.
Many different methodologies have been applied for the detection of S-nitrosothiols (RSNOs) in human biological fluids. One unsatisfactory outcome of the last 14 years of research focused on this issue is that a general consensus on reference values for physiological RSNO concentration in human blood is still missing. Consequently, both RSNO physiological function and their role in disease have not yet been clarified. Here, a summary of the values measured for RSNOs in erythrocytes, plasma, and other biological fluids is provided, together with a critical review of the most widely used analytical methods. Furthermore, some possible methodological drawbacks, responsible for the highlighted discrepancies, are evidenced.
Giustarini
Daniela
D
Department of Neuroscience, Pharmacology Section, Via A. Moro 4, University of Siena, 53100 Siena, Italy.
Milzani
Aldo
A
Dalle-Donne
Isabella
I
Rossi
Ranieri
R
D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D019411:Clinical Laboratory Techniques; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism; D026403:S-Nitrosothiols / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
D026403:S-Nitrosothiols; D009569:Nitric Oxide