SCA: symmetry-based center assignment of 2D projections of symmetric 3D objects.
A method for finding the center of cryo-EM images which correspond to the projections of a symmetric 3D structure, based on mathematical properties of symmetry adapted functions and the Fourier-Bessel transform, is presented. It is a model independent one-step procedure with no parameters to be chosen by the user. The proposed method is tested in one synthetic tetrahedral case with different noise levels and in two real cases with D7 and icosahedral symmetries.
Estrozi
Leandro Farias
LF
Institut de Biologie Structurale, UMR 5057 CNRS, CEA, UJF, 41 rue Jules, Horowitz, 38027 Grenoble, France. estrozi@ibs.fr
Trapani
Stefano
S
Navaza
Jorge
J
D018834:Chaperonin 60 / Q000737:chemistry; D020285:Cryoelectron Microscopy; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007243:Infectious bursal disease virus / Q000737:chemistry; D010363:Pattern Recognition, Automated / Q000379:methods*
D018834:Chaperonin 60