title: Diagnosis of Paracoccidioidomycosis by detection of antigen and antibody in bronchoalveolar lavage fluids.
abstract: Paracoccidioidomycosis (PCM) is a systemic infection caused by the fungus Paracoccidioides brasiliensis and is believed to be the leading cause of fungal pulmonary infection. In this study, we used an inhibition enzyme-linked immunosorbent assay to diagnose pulmonary PCM based on the detection of 43-kDa and 70-kDa molecules in bronchoalveolar lavage fluids. The results were compared with results obtained by classical methods for antibody detection.
authors.affiliation: Department of Microbiology, Immunology, and Parasitology, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.
authors.forename: Silvia Helena
authors.initials: SH
authors.lastname: Marques-da-Silva
authors.forename: Arnaldo Lopes
authors.initials: AL
authors.lastname: Colombo
authors.forename: Maria Heloisa Souza Lima
authors.initials: MH
authors.lastname: Blotta
authors.forename: Flávio
authors.initials: F
authors.lastname: Queiroz-Telles
authors.forename: Alípio Barbosa
authors.initials: AB
authors.lastname: Balthazar
authors.forename: José Daniel
authors.initials: JD
authors.lastname: Lopes
authors.forename: Zoilo Pires
authors.initials: ZP
authors.lastname: de Camargo
mesh_terms: D000908:Antibodies, Fungal / Q000032:analysis*; D000946:Antigens, Fungal / Q000032:analysis*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology* / Q000382:microbiology; D005656:Fungal Proteins / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008172:Lung Diseases, Fungal / Q000276:immunology / Q000382:microbiology; D010228:Paracoccidioides / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000175:diagnosis* / Q000382:microbiology
chemical_list: C103180:43 kDa protein, Paracoccidioides; D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D005656:Fungal Proteins; D006023:Glycoproteins