Halobacterium jilantaiense sp. nov., a halophilic archaeon isolated from a saline lake in Inner Mongolia, China.
A novel halophilic archaeon, NG4(T), was isolated from Jilantai salt lake in Inner Mongolia, China. The taxonomy of strain NG4(T) was studied by polyphasic methods. Strain NG4(T) grew at pH 5.5-8.5 and at a temperature of 22-55 degrees C. It was chemo-organotrophic, aerobic and required concentrations of 2.7-5.2 M NaCl and 0.05-0.3 M Mg(2+) for growth. Cells were Gram-negative, slender rods. Colonies on agar plates containing 25 % (w/v) total salts were red, elevated and round. According to 16S rRNA gene sequence similarity, strain NG4(T) was phylogenetically related to Halobacterium salinarum DSM 3754(T) (98.2 %) and Halobacterium noricense A1(T) (97.3 %). The DNA G+C content was 64.2 mol%. DNA-DNA relatedness values with Hbt. salinarum DSM 3754(T) and Hbt. noricense A1(T) were 47 and 35 %, respectively. The polar lipids of strain NG4(T) consisted of phosphatidylglycerol, methylated phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether, sulfated triglycosyl diether and sulfated tetraglycosyl diether. It was concluded that strain NG4(T) represents a novel species of the genus Halobacterium, for which the name Halobacterium jilantaiense sp. nov. is proposed. The type strain is NG4(T) (=CGMCC 1.5337(T)=JCM 13558(T)).
Yang
Yong
Y
Graduate School of Chinese Academy of Sciences, Beijing 100049, China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, PR China.
Cui
Heng-Lin
HL
Graduate School of Chinese Academy of Sciences, Beijing 100049, China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, PR China.
Zhou
Pei-Jin
PJ
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, PR China.
Liu
Shuang-Jiang
SJ
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, PR China.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D019641:DNA, Archaeal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D020459:Genes, rRNA; D006217:Halobacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013045:Species Specificity
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride