Post-meiotic shifts in HSPA2/HSP70.2 chaperone activity during mouse spermatogenesis.
HSPA2 (formerly HSP70.2) is a testis-specific member of the HSP70 family known to play a critical role in the completion of meiosis during male germ cell differentiation. Although abundantly present in post-meiotic cells, its function during spermiogenesis remained obscure. Here, using a global proteomic approach to identify genome-organizing proteins in condensing spermatids, we discovered an unexpected role for HSPA2, which acquires new functions and becomes tightly associated with major spermatid DNA-packaging proteins, transition proteins 1 and 2. Hence, HSPA2 is identified here as the first transition protein chaperone, and these data shed a new light on the yet totally unknown process of genome-condensing structure assembly in spermatids.
Govin
Jérôme
J
INSERM, U309, Institut Albert Bonniot, F-38700 Grenoble, France.
Caron
Cécile
C
Escoffier
Emmanuelle
E
Ferro
Myriam
M
Kuhn
Lauriane
L
Rousseaux
Sophie
S
Eddy
Edward M
EM
Garin
Jérôme
J
Khochbin
Saadi
S
D000818:Animals; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D042003:DNA Packaging; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008540:Meiosis; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding; D040901:Proteomics; D013087:Spermatids / Q000166:cytology / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*
D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; C490434:Hspa2 protein, mouse; D009687:Nuclear Proteins; C489505:Tnp2 protein, mouse; C053886:spermatid transition proteins