Adaptive potential of human biceps femoris muscle demonstrated by histochemical, immunohistochemical and mechanomyographical methods.
The goal of this study was to estimate the ability of biceps femoris (BF) muscle, a hamstring muscle crucial for biarticulate movement, to adapt to changed functional demands. For this purpose and due to ethical reasons, in a group of healthy sedentary men and of 15 sprinters, a non-invasive mechanomyography (MMG) method was used to measure the muscle twitch contraction times (Tc). These correlate with the proportions of slow and fast fibres in the muscle. To further elucidate the data obtained by MMG method and to obtain reference data for the muscle, the fiber type proportions in autoptic samples of BF in sedentary young men were determined according to histochemical reaction for myofibrillar adenosine triphosphatase (mATPase). In one BF sample also myosin heavy chain (MyHC) isoform expression was demonstrated immunohistochemically. With MMG we indirectly demonstrated that biceps femoris muscle has a strong potential to transform into faster contracting muscle after sprint training, since the average Tc in sprinters was much lower (19.5 +/- 2.3 ms) than in the sedentary group (30.25 +/- 3.5 ms). The results of the histochemical and immunohistochemical analysis of BF muscle also imply a high adapting potential of this muscle. Beside type 1, 2a and 2x (2b) fibres a relatively high proportion of intermediate type 2c fibres (5.7% +/- 0.7), which co-expressed MyHC-1 and -2a, was found. Therefore, type 2c might represent a potential pool of fibres, capable of transformation either to slow type 1 or to fast type 2a in order to tune the functional response of BF muscle according to the actual functional demands of the organism.
Dahmane
Raja
R
Institute of Anatomy, Medical Faculty, University of Ljubljana, Korytkova 2, Ljubljana, Slovenia. raja.dahmane@guest.arnes.si
Djordjevic
Srdjan
S
Smerdu
Vika
V
D000222:Adaptation, Physiological; D000251:Adenosine Triphosphatases / Q000032:analysis; D000328:Adult; D016022:Case-Control Studies; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009119:Muscle Contraction; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology; D018482:Muscle, Skeletal / Q000737:chemistry / Q000502:physiology*; D009213:Myography; D018995:Myosin Heavy Chains / Q000032:analysis; D010806:Physical Education and Training; D012420:Running
D000251:Adenosine Triphosphatases; D018995:Myosin Heavy Chains