title: [The effect of Sairei-to on oxygen-induced retinal neovascularization in the neonatal rat].
abstract: OBJECTIVE
  To study the effect of Sairei-to (ST), a Japanese traditional medicine, on oxygen-induced retinopathy (OIR) in rats.
  
  
  METHODS
  OIR was induced by maintaining Sprague-Dawley neonatal rats in 80% oxygen for 12 days. The rats were treated once daily with oral administration of 0.75 g/kg (n = 9), 1.5 g/kg (n = 13) of ST in water, or water alone (WA, n = 13) at 5 mL/ kg body weight from day 6 to day 17. On day 18, retinal samples were collected. Retinal neovascularization (NV) was assessed by the NV score, and by the percentage of avascular area (% AVA), using a method previously reported. The number of severe retinal NV cases (NV > or = 9) was compared. The retinal vascular endothelial growth factor (VEGF) concentrations were measured with an immunoassay kit, at 0, 12, 24, 72 and 144 hours after oxygenation.
  
  
  RESULTS
  NV score and % AVA decreased in the ST treated group compared to the WA group. However, severe NV was seen in five cases of WA and in one case of the ST treated group. Thus severe NV was inhibited significantly by ST treatment (p = 0.0185). Retinal VEGF did not differ between groups at any time points.
  
  
  CONCLUSIONS
  These data suggest that severe NV in OIR is inhibited by ST treatment.
authors.affiliation: Department of Ophthalmology, Showa University School of Medicine, Tokyo, Japan. kosuge@med.showa-u.ac.jp
authors.forename: Shotaro
authors.initials: S
authors.lastname: Kosuge
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Onda
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Takenaka
authors.forename: Takako
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ueda
authors.forename: Maki
authors.initials: M
authors.lastname: Kamegawa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Tsukahara
authors.forename: Hajime
authors.initials: H
authors.lastname: Yasuhara
authors.forename: Ryohei
authors.initials: R
authors.lastname: Koide
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D010100:Oxygen / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000737:chemistry; D015861:Retinal Neovascularization / Q000188:drug therapy* / Q000209:etiology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D004365:Drugs, Chinese Herbal; D042461:Vascular Endothelial Growth Factor A; C080903:sairei-to; D010100:Oxygen