Conversion of capsular polysaccharide, involved in pellicle formation, to extracellular polysaccharide by galE deletion in Acetobacter tropicalis.
Acetobacter tropicalis SKU1100 produces a pellicle-forming capsular polysaccharide (CPS), consisting of galactose, glucose, and rhamnose. We cloned the galE gene, a UDP-galactose synthesis gene, from A. tropicalis SKU1100 by PCR. A galE-disruptant was prepared and found not to produce CPS and thus not to form a pellicle under the static condition. Instead, the DeltagalE mutant secreted an extracellular polysaccharide (EPS), which was purified and found to have a unique character, different from the original CPS.
Deeraksa
Arpaporn
A
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.
Moonmangmee
Somporn
S
Toyama
Hirohide
H
Adachi
Osao
O
Matsushita
Kazunobu
K
D000091:Acetobacter / Q000201:enzymology* / Q000378:metabolism; D016667:Bacterial Capsules / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D017384:Sequence Deletion; D014534:UDPglucose 4-Epimerase / Q000235:genetics* / Q000378:metabolism*
D011135:Polysaccharides, Bacterial; D014534:UDPglucose 4-Epimerase; C020551:galactose epimerase