Affinity liquid chromatography method for the quantification of immunoglobulin G in bovine colostrum powders.
An affinity liquid chromatography (LC) method for the determination of bovine immunoglobulin G (IgG), using protein G coupled to an agarose support, was modified to permit the quantification of IgG in colostrum-based powders. Sample preparation included pH adjustment to 4.6 to precipitate casein and denatured whey protein. The method was applied to a range of colostrum powders and was compared with the alternative independent methods of surface plasmon resonance immunoassay, radial immunodiffusion, and reversed-phase LC. The method was rapid, and performance parameters included a working range of 10-150 microg IgG and precision relative standard deviation values of <10%.
Copestake
David E J
DE
Fonterra-Palmerston North, Dairy Farm Rd, Private Bag 11029, Palmerston North, New Zealand.
Indyk
Harvey E
HE
Otter
Don E
DE
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods* / Q000706:statistics & numerical data; D003126:Colostrum / Q000737:chemistry* / Q000276:immunology*; D005260:Female; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis*; D009419:Nerve Tissue Proteins; D011208:Powders; D011247:Pregnancy; D020349:Surface Plasmon Resonance
C029152:G-substrate; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D011208:Powders