Synthesis and G-quadruplex binding studies of new 4-N-methylpyridinium porphyrins.
A series of cationic porphyrins carrying 1-3 meso-N-pyridinium groups has been synthesised, and their binding to G-quadruplex DNA has been explored by surface plasmon resonance (SPR) and circular dichroism spectroscopy. Two trans substituents appear to be sufficient for tight binding; preferential binding to the anti-parallel intramolecular human telomeric DNA was observed for the A2trans and A3 porphyrins. The A2trans is able to induce the formation of an anti-parallel G-quadruplex in a K+ free solution, mimicking the effect of a molecular chaperone.
Gonçalves
Diana P N
DP
University Chemical Laboratory, Lensfield Road, Cambridge, UKCB2 1EW.
Ladame
Sylvain
S
Balasubramanian
Shankar
S
Sanders
Jeremy K M
JK
D002942:Circular Dichroism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D011166:Porphyrins / Q000138:chemical synthesis* / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000138:chemical synthesis* / Q000737:chemistry*
D011166:Porphyrins; D011726:Pyridinium Compounds