Human mesenchymal stem cells favour healing of the cutaneous radiation syndrome in a xenogenic transplant model.
It has been suggested that human mesenchymal stem cells (hMSC) could be used to repair numerous injured tissues. We have studied the potential use of hMSC to limit radiation-induced skin lesions. Immunodeficient NOD/SCID mice were locally irradiated to the leg (30 Gy, dose rate 2.7 Gy/min) using a (60)Co source to induce a severe skin lesion. Cultured bone marrow hMSC were delivered intravenously to the mice. The irradiated skin samples were studied for the presence of the human cells, the severity of the lesions and the healing process. Macroscopic analysis and histology results showed that the lesions were evolving to a less severe degree of radiation dermatitis after hMSC transplant when compared to irradiated non-transplanted controls. Clinical scores for the studied skin parameters of treated mice were significantly improved. A faster healing was observed when compared to untreated mouse. Immunohistology and polymerase chain reaction analysis provided evidence that the human cells were found in the irradiated area. These results suggest a possible use of hMSC for the treatment of the early phase of the cutaneous radiation syndrome. A successful transplant of stem cells and subsequent reduction in radiation-induced complication may open the road to completely new strategies in cutaneous radiation syndrome therapy.
François
Sabine
S
Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, Fontenay aux Roses CEDEX, France. sabine.francois@irsn.fr
Mouiseddine
Moubarak
M
Mathieu
Noëlle
N
Semont
Alexandra
A
Monti
Pascale
P
Dudoignon
Nicolas
N
Saché
Amandine
A
Boutarfa
Asma
A
Thierry
Dominique
D
Gourmelon
Patrick
P
Chapel
Alain
A
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D011855:Radiodermatitis / Q000473:pathology / Q000628:therapy*; D013577:Syndrome; D014183:Transplantation, Heterologous; D014945:Wound Healing