Effects of nucleotides on the interaction of renin with GlcNAc 2-epimerase (renin binding protein, RnBP).
Renin binding protein (RnBP), a cellular renin inhibitor, was identified as an enzyme, GlcNAc 2-epimerase. Recombinant RnBP inhibited porcine renin activity in a dose dependent manner. However, the inhibition was neutralized by nucleotides, such as ATP, dATP, dGTP, dCTP or dTTP. Moreover, ATP inhibited the formation of hetero-complex of renin with RnBP, called high molecular weight (HMW) renin. On the other hand, N-ethylmaleimide (NEM), a SH-alkylating reagent inhibited the GlcNAc 2-epimerase activity concomitant with the decaying of the dimer to the monomer of the enzyme. The inhibition was modulated in the presence of ATP. These results indicate that nucleotides stabilize the dimeric form RnBP (GlcNAc 2-epimerase) and inhibited the formation of the renin-RnBP hetero complex, HMW renin.
Takahashi
Saori
S
Akita Research Institute for Food and Brewing, 4-26, Sanuki, Arayamachi, Akita 010-1623.
Hori
Kazuyuki
K
Ogasawara
Hironobu
H
Hiwatashi
Kazuyuki
K
Sugiyama
Toshihiro
T
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D019281:Dimerization; D005033:Ethylmaleimide / Q000494:pharmacology; D008958:Models, Molecular; D009711:Nucleotides / Q000494:pharmacology*; D020836:Protein Structure, Quaternary / Q000187:drug effects; D013552:Swine; D013820:Thermolysin / Q000378:metabolism; D000092502:Renin Inhibitors
D000255:Adenosine Triphosphate; D002238:Carbohydrate Epimerases; D002352:Carrier Proteins; D005033:Ethylmaleimide; D009711:Nucleotides; D013820:Thermolysin; D000092502:Renin Inhibitors; C498259:RENBP protein, human