title: Proteomics characterization of protein adsorption onto hemodialysis membranes.
abstract: Protein-adsorptive properties are a key feature of membranes used for hemodialysis treatment. Protein adsorption is vital to the biocompatibility of a membrane material and influences membrane's performance. The object of the present study is to investigate membrane biocompatibility by correlating the adsorbed proteome repertoire with structural feature of the membrane surfaces. Minidialyzers of identical structural characteristics composed of either cellulose diacetate or ethylenevinyl alcohol materials were employed to develop an ex vivo apparatus to investigate protein adsorption. Adsorbed proteins were eluted by a strong chaotropic buffer condition and investigated by 2-DE coupled to both MALDI-TOF mass spectrometry (MS) mass fingerprinting and fragmentation analysis on a nanoLC-MS/MS hybrid instrument. Membrane surface characterization included evaluation of roughness (atomic force microscopy), elemental chemical composition (X-ray-photoelectron-spectroscopy), and hydrophilicity (pulsed nuclear magnetic resonance). The present study identifies a number of different proteins as common or characteristic of filter material interaction, showing that proteomic techniques are a promising approach for the investigation of proteins surface-adsorbed onto hemodialysis membrane. Proteomic analysis enables the characterization of protein layers of unknown composition.
authors.affiliation: Istituto di Clinica Nefrologica, Dipartimento di Medicina, Università G. D'Annunzio di Chieti-Pescara, Centro Studi sull'Invecchiamento (Ce.S.I.), Chieti, Italy. m.bonomini@nephro.unich.it
authors.forename: Mario
authors.initials: M
authors.lastname: Bonomini
authors.forename: Barbara
authors.initials: B
authors.lastname: Pavone
authors.forename: Vittorio
authors.initials: V
authors.lastname: Sirolli
authors.forename: Francesca
authors.initials: F
authors.lastname: Del Buono
authors.forename: Moreno
authors.initials: M
authors.lastname: Di Cesare
authors.forename: Piero
authors.initials: P
authors.lastname: Del Boccio
authors.forename: Luigi
authors.initials: L
authors.lastname: Amoroso
authors.forename: Carmine
authors.initials: C
authors.lastname: Di Ilio
authors.forename: Paolo
authors.initials: P
authors.lastname: Sacchetta
authors.forename: Giorgio
authors.initials: G
authors.lastname: Federici
authors.forename: Andrea
authors.initials: A
authors.lastname: Urbani
mesh_terms: D000327:Adsorption; D001798:Blood Proteins / Q000032:analysis*; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D011145:Polyvinyls / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D006435:Renal Dialysis / Q000295:instrumentation*; D013499:Surface Properties
chemical_list: D001798:Blood Proteins; D008567:Membranes, Artificial; D011145:Polyvinyls; D020543:Proteome; C020320:ethylene-vinyl alcohol copolymer; D002482:Cellulose; C026170:cellulose diacetate