title: Stomach-selective gene transfer following the administration of naked plasmid DNA onto the gastric serosal surface in mice.
abstract: The purpose of the present study was to achieve a stomach-selective gene transfer following the administration of naked plasmid DNA (pDNA) onto the gastric serosal surface in mice. Gene expression in the stomach and other tissues was evaluated by firefly luciferase activity. Six hours after gastric serosal surface instillation of naked pDNA, high gene expression in the stomach was observed. On the contrary, intravenous and intraperitoneal injection of naked pDNA exhibited no detectable gene expression. Following instillation of naked pDNA onto the gastric serosal surface, gene expression in the stomach was significantly higher than in other tissues. Gene expression in the stomach was highest 12 h after the instillation and thereafter decreased gradually. Utilizing a glass-made diffusion cell that is able to limit the contact dimension between the gastric serosal surface and the naked pDNA solution administered, site-specific gene expression in the stomach was achieved. This novel gene transfer method is expected to be a safe and effective treatment against serious stomach diseases.
authors.affiliation: Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan. junzo@nagasaki-u.ac.jp
authors.forename: Junzo
authors.initials: J
authors.lastname: Nakamura
authors.forename: Shintaro
authors.initials: S
authors.lastname: Fumoto
authors.forename: Keiko
authors.initials: K
authors.lastname: Shoji
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Kodama
authors.forename: Junya
authors.initials: J
authors.lastname: Nishi
authors.forename: Mikiro
authors.initials: M
authors.lastname: Nakashima
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sasaki
authors.forename: Koyo
authors.initials: K
authors.lastname: Nishida
mesh_terms: D000818:Animals; D004247:DNA / Q000008:administration & dosage*; D005753:Gastric Mucosa / Q000378:metabolism*; D015316:Genetic Therapy / Q000379:methods*; D008297:Male; D051379:Mice; D010957:Plasmids; D013272:Stomach Diseases / Q000628:therapy*
chemical_list: D004247:DNA