title: Roles of PriA protein and double-strand DNA break repair functions in UV-induced restriction alleviation in Escherichia coli.
abstract: It has been widely considered that DNA modification protects the chromosome of bacteria E. coli K-12 against their own restriction-modification systems. Chromosomal DNA is protected from degradation by methylation of target sequences. However, when unmethylated target sequences are generated in the host chromosome, the endonuclease activity of the EcoKI restriction-modification enzyme is inactivated by the ClpXP protease and DNA is protected. This process is known as restriction alleviation (RA) and it can be induced by UV irradiation (UV-induced RA). It has been proposed that chromosomal unmethylated target sequences, a signal for the cell to protect its own DNA, can be generated by homologous recombination during the repair of damaged DNA. In this study, we wanted to further investigate the genetic requirements for recombination proteins involved in the generation of unmethylated target sequences. For this purpose, we monitored the alleviation of EcoKI restriction by measuring the survival of unmodified lambda in UV-irradiated cells. Our genetic analysis showed that UV-induced RA is dependent on the excision repair protein UvrA, the RecA-loading activity of the RecBCD enzyme, and the primosome assembly activity of the PriA helicase and is partially dependent on RecFOR proteins. On the basis of our results, we propose that unmethylated target sequences are generated at the D-loop by the strand exchange of two hemi-methylated duplex DNAs and subsequent initiation of DNA replication.
authors.affiliation: Department of Molecular Biology, Faculty of Science, University of Zagreb, Croatia. ivanai@irb.hr
authors.forename: Ivana
authors.initials: I
authors.lastname: Ivancić-Bacće
authors.forename: Ignacija
authors.initials: I
authors.lastname: Vlasić
authors.forename: Gordana
authors.initials: G
authors.lastname: Cogelja-Cajo
authors.forename: Krunoslav
authors.initials: K
authors.lastname: Brcić-Kostić
authors.forename: Erika
authors.initials: E
authors.lastname: Salaj-Smic
mesh_terms: D002876:Chromosomes, Bacterial; D053903:DNA Breaks, Double-Stranded; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair; D004261:DNA Replication / Q000528:radiation effects; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D043211:Exodeoxyribonuclease V / Q000235:genetics / Q000378:metabolism; D011836:Radiation Tolerance; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C489340:recF protein, E coli; D011938:Rec A Recombinases; D043211:Exodeoxyribonuclease V; D004262:DNA Restriction Enzymes; C506743:priA protein, E coli; D004265:DNA Helicases