title: Proposal of Roseburia faecis sp. nov., Roseburia hominis sp. nov. and Roseburia inulinivorans sp. nov., based on isolates from human faeces.
abstract: Seven recently cultured bacterial isolates, although similar in their 16S rRNA gene sequences to Roseburia intestinalis L1-82(T) (DSM 14610(T)), were not sufficiently related for inclusion within existing species, forming three separate clusters in a 16S rRNA gene phylogenetic tree. The isolates, which were obtained from human stools, were Gram-variable or Gram-negative, strictly anaerobic, slightly curved rods; cells from all strains measured approximately 0.5x1.5-5.0 mum and were motile. Two strains belonging to one cluster (A2-181 and A2-183(T)) were the only strains that were able to grow on glycerol and that failed to grow on any of the complex substrates tested (inulin, xylan and amylopectin). Strains belonging to a second cluster (represented by M6/1 and M72/1(T)) differed from the other isolates in their ability to grow on sorbitol. Isolates belonging to a third cluster (L1-83 and A2-194(T)) were the only strains that failed to grow on xylose and that gave good growth on inulin (strains M6/1 and M72/1(T) gave weak growth). All strains were net acetate utilizers. The DNA G+C contents of representative Roseburia strains A2-183(T), A2-194(T), M72/1(T) and R. intestinalis L1-82(T) were 47.4, 41.4, 42.0 and 42.6 mol%, respectively. Based on 16S rRNA gene sequence similarity, three novel Roseburia species are proposed, with the names Roseburia hominis sp. nov. (type strain A2-183(T)=DSM 16839(T)=NCIMB 14029(T)), Roseburia inulinivorans sp. nov. (type strain A2-194(T)=DSM 16841(T)=NCIMB 14030(T)) and Roseburia faecis sp. nov. (type strain M72/1(T)=DSM 16840(T)=NCIMB 14031(T)).
authors.affiliation: Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Sylvia H
authors.initials: SH
authors.lastname: Duncan
authors.affiliation: Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Rustam I
authors.initials: RI
authors.lastname: Aminov
authors.affiliation: Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Karen P
authors.initials: KP
authors.lastname: Scott
authors.affiliation: Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Petra
authors.initials: P
authors.lastname: Louis
authors.affiliation: National Animal Disease Center, USDA ARS, PO Box 70, 2300 Dayton Road, Ames, IA 50010, USA.
authors.forename: Thaddeus B
authors.initials: TB
authors.lastname: Stanton
authors.affiliation: Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Flint
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D001482:Base Composition; D050260:Carbohydrate Metabolism; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S