title: Crinamine from Crinum asiaticum var. japonicum inhibits hypoxia inducible factor-1 activity but not activity of hypoxia inducible factor-2.
abstract: In a search for natural product inhibitors of hypoxia inducible factor-1 (HIF-1) function, crinamine (1), a crinane type alkaloid, showed potent dose dependent inhibition (IC50 = 2.7 microM) of HIF-1alpha in a cell-based reporter gene assay. Crinamine (1) was isolated from the aerial parts of Crinum asiaticum var. japonicum together with lycorine (2), norgalanthamine (3) and epinorgalanthamine (4). The other components (2-4) showed no significant inhibition of HIF-1alpha induced transcriptional activity.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, Korea. yhk@cnu.ac.kr
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Park
authors.forename: Mi Hyun
authors.initials: MH
authors.lastname: Park
authors.forename: Uranchimeg
authors.initials: U
authors.lastname: Badarch
authors.forename: Girma Moges
authors.initials: GM
authors.lastname: Woldemichael
authors.forename: John Albert
authors.initials: JA
authors.lastname: Beutler
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000494:pharmacology*; D051792:Basic Helix-Loop-Helix Proteins; D045744:Cell Line, Tumor; D031407:Crinum / Q000737:chemistry*; D006801:Humans; D051793:Hypoxia-Inducible Factor 1 / Q000037:antagonists & inhibitors*; D007212:Indolizines / Q000494:pharmacology*; D052247:Nitric Oxide Synthase Type II / Q000502:physiology; D014157:Transcription Factors / Q000037:antagonists & inhibitors*; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D047151:Amaryllidaceae Alkaloids; D051792:Basic Helix-Loop-Helix Proteins; D051793:Hypoxia-Inducible Factor 1; D007212:Indolizines; D052247:Nitric Oxide Synthase Type II; D014157:Transcription Factors; D000099162:Endothelial PAS Domain-Containing Protein 1; C411129:crinamine