[Quantification for biofilm formation of pseudomonas aeruginosa isolates and their genotypic analysis].
OBJECTIVE
To probe the correlation between the ability of biofilm formation of Pseudomonas aeruginosa isolates and their genotypes.


METHODS
Forty-eight Pseudomonas aeruginosa isolates were tested for their biofilm formation with a modified microtiter test and were analyzed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The percent similarity between their genetic fingerprints and cluster analysis was performed and worked out using computer software.


RESULTS
Forty-eight Pseudomonas aeruginosa isolates demonstrated different abilities of biofilm formation in vitro. And the 48 isolates with different abilities of biofilm formation showed different genotypes in fingerprints by ERIC-PCR. Among the 48 isolates of Pseudomonas aeruginosa tested, five clusters (A, B, C, D, E), were identified at the 17% genetic similarity level. The isolates with strong ability of biofilm formation were in D cluster at the 42% genetic similarity level.


CONCLUSIONS
Most of the 48 PA isolates tested formed strong biofilm, and their genotypes were of correlation.
Cheng
Xi
X
Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Yang
Wei-qing
WQ
Shi
Lei
L
Yin
Xiao-lin
XL
Xie
Yi
Y
Zeng
Wei
W
Li
Xin-hui
XH
Su
Jian-yu
JY
Kou
Ya-li
YL
Jia
Wen-xiang
WX
D018441:Biofilms; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000502:physiology*; D019105:Random Amplified Polymorphic DNA Technique
D004269:DNA, Bacterial