Experimental infection of Culex annulirostris, Culex gelidus, and Aedes vigilax with a yellow fever/Japanese encephalitis virus vaccine chimera (ChimeriVax-JE).
Australian mosquitoes from which Japanese encephalitis virus (JEV) has been recovered (Culex annulirostris, Culex gelidus, and Aedes vigilax) were assessed for their ability to be infected with the ChimeriVax-JE vaccine, with yellow fever vaccine virus 17D (YF 17D) from which the backbone of ChimeriVax-JE vaccine is derived and with JEV-Nakayama. None of the mosquitoes became infected after being fed orally with 6.1 log(10) plaque-forming units (PFU)/mL of ChimeriVax-JE vaccine, which is greater than the peak viremia in vaccinees (mean peak viremia = 4.8 PFU/mL, range = 0-30 PFU/mL of 0.9 days mean duration, range = 0-11 days). Some members of all three species of mosquito became infected when fed on JEV-Nakayama, but only Ae. vigilax was infected when fed on YF 17D. The results suggest that none of these three species of mosquito are likely to set up secondary cycles of transmission of ChimeriVax-JE in Australia after feeding on a viremic vaccinee.
Reid
Mark
M
Australian Army Malaria Institute, Brisbane, Queensland, Australia. markreid2@optusnet.com.au
Mackenzie
Donna
D
Baron
Andrew
A
Lehmann
Natalie
N
Lowry
Kym
K
Aaskov
John
J
Guirakhoo
Farshad
F
Monath
Thomas P
TP
D000330:Aedes / Q000276:immunology / Q000821:virology*; D000818:Animals; D001315:Australia; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003465:Culex / Q000276:immunology / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D012367:RNA, Viral / Q000032:analysis / Q000737:chemistry; D014709:Vero Cells; D014765:Viral Vaccines / Q000032:analysis; D053060:West Nile Virus Vaccines; D015005:Yellow fever virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*
C445780:ChimeriVax; D012367:RNA, Viral; D014765:Viral Vaccines; D053060:West Nile Virus Vaccines