title: Regulation of the beta-hydroxyacyl ACP dehydratase gene of Picea mariana by alternative splicing.
abstract: The gene for beta-hydroxyacyl ACP dehydratase, a de novo fatty acid biosynthetic enzyme, was cloned from Picea mariana (black spruce) and consists of five exons and four introns. The first intron of the beta-hydroxyacyl ACP dehydratase mRNA is alternatively spliced. Retention of intron 1 in splice variants results in truncation of the beta-hydroxyacyl ACP dehydratase ORF at a premature termination codon. In addition, splicing of intron 1 was found to be associated with cold temperature. mRNAs retaining intron 1 increase with seed imbibition at 22 degrees C but not 4 degrees C, whereas, splicing of intron 1 increases in winter weeks with temperatures below freezing. These results provide evidence that alternative splicing may also contribute to regulation of lipid biosynthesis in Picea mariana.
authors.affiliation: Canadian Forest Service, Natural Resources Canada, P.O. Box 4000, Fredericton, NB, E3B 5P7, Canada. htai@nrcan.gc.ca
authors.forename: Helen H
authors.initials: HH
authors.lastname: Tai
authors.forename: Martin
authors.initials: M
authors.lastname: Williams
authors.forename: Abhinav
authors.initials: A
authors.lastname: Iyengar
authors.forename: Jessica
authors.initials: J
authors.lastname: Yeates
authors.forename: Tannis
authors.initials: T
authors.lastname: Beardmore
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D004746:Enoyl-CoA Hydratase / Q000235:genetics*; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D028222:Picea / Q000201:enzymology / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D022821:RNA Splice Sites / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012621:Seasons; D012639:Seeds / Q000201:enzymology / Q000235:genetics; D016415:Sequence Alignment; D013696:Temperature
chemical_list: D010940:Plant Proteins; D022821:RNA Splice Sites; D012333:RNA, Messenger; D004746:Enoyl-CoA Hydratase