High-molecular weight adiponectin isoforms increase after biliopancreatic diversion in obese subjects.
OBJECTIVE
Our objective was to test the effect of biliopancreatic diversion (BDP) in adiponectin multimerization. Adiponectin, the major protein secreted by adipose tissue, circulates in plasma in different isoforms. The most clinically relevant oligomers are high-molecular weight (HMW) multimers and low-molecular weight (LMW) trimers. Contrasting data on the effect of weight loss on adiponectin isoforms have been reported.


METHODS
We measured total plasma adiponectin and HMW and LMW adiponectin oligomers (by Western blot analysis) before and 1 month after BPD, in 18 severely obese subjects.


RESULTS
One month after BPD, body weight decreased approximately 11%. Total adiponectin showed significant increase after BPD. In addition, we found a significant increase in HMW (percentage) adiponectin oligomers. We found a significant inverse correlation between HMW (percentage) and BMI before and after BPD. Homeostasis model of assessment-insulin resistance decreased significantly after the BPD, without any significant correlation with total serum adiponectin and adiponectin oligomers.


CONCLUSIONS
A moderate weight loss after BPD increases total and HMW adiponectin oligomers. The significant correlation between BMI and HMW (percentage) adiponectin oligomers but not between BMI and total adiponectin might indicate a role of body fat mass in regulation of adiponectin multimerization. These data suggest that HMW oligomers represent a very sensitive parameter to short-term BMI changes after BPD.
Salani
Barbara
B
Department of Endocrinology, University of Genova, Genova, Italy.
Briatore
Lucia
L
Andraghetti
Gabriella
G
Adami
Gian Franco
GF
Maggi
Davide
D
Cordera
Renzo
R
D052242:Adiponectin / Q000097:blood / Q000737:chemistry*; D015904:Biliopancreatic Diversion; D015153:Blotting, Western; D015992:Body Mass Index; D005260:Female; D006801:Humans; D008297:Male; D008970:Molecular Weight; D009767:Obesity, Morbid / Q000097:blood* / Q000601:surgery*; D020033:Protein Isoforms; D012727:Sex Characteristics; D015431:Weight Loss / Q000502:physiology*
D052242:Adiponectin; D020033:Protein Isoforms