title: Identification of structural and catalytic classes of highly conserved amino acid residues in lysine 2,3-aminomutase.
abstract: Lysine 2,3-aminomutase (LAM) from Clostridium subterminale SB4 catalyzes the interconversion of (S)-lysine and (S)-beta-lysine by a radical mechanism involving coenzymatic actions of S-adenosylmethionine (SAM), a [4Fe-4S] cluster, and pyridoxal 5'-phosphate (PLP). The enzyme contains a number of conserved acidic residues and a cysteine- and arginine-rich motif, which binds iron and sulfide in the [4Fe-4S] cluster. The results of activity and iron, sulfide, and PLP analysis of variants resulting from site-specific mutations of the conserved acidic residues and the arginine residues in the iron-sulfide binding motif indicate two classes of conserved residues of each type. Mutation of the conserved residues Arg134, Asp293, and Asp330 abolishes all enzymatic activity. On the basis of the X-ray crystal structure, these residues bind the epsilon-aminium and alpha-carboxylate groups of (S)-lysine. However, among these residues, only Asp293 appears to be important for stabilizing the [4Fe-4S] cluster. Members of a second group of conserved residues appear to stabilize the structure of LAM. Mutations of arginine 130, 135, and 136 and acidic residues Glu86, Asp165, Glu236, and Asp172 dramatically decrease iron and sulfide contents in the purified variants. Mutation of Asp96 significantly decreases iron and sulfide content. Arg130 or Asp172 variants display no detectable activity, whereas variants mutated at the other positions display low to very low activities. Structural roles are assigned to this latter class of conserved amino acids. In particular, a network of hydrogen bonded interactions of Arg130, Glu86, Arg135, and the main chain carbonyl groups of Cys132 and Leu55 appears to stabilize the [4Fe-4S] cluster.
authors.affiliation: Department of Biochemistry, University of Wisconsin, 1710 University Avenue, Madison, Wisconsin 53726, USA.
authors.forename: Dawei
authors.initials: D
authors.lastname: Chen
authors.forename: Perry A
authors.initials: PA
authors.lastname: Frey
authors.forename: Bryan W
authors.initials: BW
authors.lastname: Lepore
authors.forename: Dagmar
authors.initials: D
authors.lastname: Ringe
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Ruzicka
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D002384:Catalysis; D003013:Clostridium / Q000201:enzymology; D017124:Conserved Sequence; D017931:DNA Primers; D019751:Intramolecular Transferases / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism
chemical_list: D017931:DNA Primers; D021122:Protein Subunits; D019751:Intramolecular Transferases; C021518:lysine 2,3-aminomutase