Selection of photosynthetic bacterium Rhodobacter sphaeroides 14F for polyhydroxyalkanoate production with two-stage aerobic dark cultivation.
Polyhydroxyalkanoate (PHA) production abilities in a two-stage aerobic dark culture of photosynthetic bacteria were investigated at relatively high temperatures (37-40 degrees C). A 14F strain, identified as Rhodobacter sphaeroides, showed the highest PHA production (3.5 g/l PHA with 60% PHA content). Its productivity was 2-3 times higher than those of other photosynthetic bacteria.
Lorrungruang
Chortip
C
Department of Microbiology, Faculty of Science, Kasetsart University, 50 Paholyothin Rd, Bangkok 10900, Thailand.
Martthong
Jeerapa
J
Sasaki
Ken
K
Noparatnaraporn
Napavarn
N
D000217:Acyltransferases / Q000737:chemistry*; D000421:Alcaligenes / Q000378:metabolism; D000473:Alkanes / Q000737:chemistry*; D001419:Bacteria / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D003624:Darkness; D005632:Fructose / Q000737:chemistry; D008027:Light; D010788:Photosynthesis; D010957:Plasmids / Q000378:metabolism; D012242:Rhodobacter sphaeroides / Q000201:enzymology*; D013696:Temperature; D013997:Time Factors
D000473:Alkanes; D005632:Fructose; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase