Distinct role of IL-3 promoter and enhancer region in murine mast cells.
Crosslinking of Fcvarepsilon receptor on mast cells induces IL-3 gene expression with the concentration dependent of intracellular calcium, but its regulatory mechanism remains unclear. Here, we found that phorbol 12-myristate 13-acetate (PMA) alone did not induce IL-3 gene expression, but potentiated A23187-induced IL-3 gene expression. Interestingly, the A23187-induced IL-3 promoter activity was suppressed by PMA, but it was enhanced when IL-3 promoter contained enhancer region, a DH site. While IL-3 mRNA expression was increased by A23187 and PMA in a dose-dependent manner, the promoter activity appeared all or none in all doses of A23187 and PMA. IL-3 promoter region between -293 and -150bp was responsible for A23187-induced gene expression and PMA- or cyclosporin A (CsA)-mediated suppression. Taken together, IL-3 gene expression was primarily regulated at the transcriptional level, which was differentially controlled by a restricted promoter and enhancer region.
Ko
Chang-Bo
CB
School of Biological Sciences and Technology, Hormone Research Institute, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
Lee
Bok-Soo
BS
Cha
Seok-Ho
SH
Sul
Donggeun
D
Paik
Sang-Gi
SG
Kang
Hyung-Sik
HS
D000818:Animals; D001483:Base Sequence; D000001:Calcimycin / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D003850:Deoxyribonuclease I / Q000737:chemistry; D004742:Enhancer Elements, Genetic / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D007377:Interleukin-3 / Q000235:genetics*; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
D007377:Interleukin-3; D012333:RNA, Messenger; D000001:Calcimycin; C016658:phorbolol myristate acetate; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D016572:Cyclosporine; D003850:Deoxyribonuclease I; D013755:Tetradecanoylphorbol Acetate