title: The role of UvrD in RecET-mediated illegitimate recombination in Escherichia coli.
abstract: To study the mechanism of RecET-mediated illegitimate recombination, we examined the formation of lambdabio-transducing phage in Escherichia coli in the presence or absence of UV irradiation. We have previously reported that coexpression of RecE and RecT enhances the frequency of recA-independent illegitimate recombination. RecJOR proteins are required for this RecET-mediated illegitimate recombination, and RecQ suppresses it. Here, we showed that the frequencies of both spontaneous and UV-induced RecET-mediated illegitimate recombination events are reduced by a uvrD mutation. It should be noted that UvrD is required for illegitimate recombination only in the presence, but not in the absence, of RecET. In contrast, frequencies of RecET-mediated illegitimate recombination were not affected by ruvAB, ruvC, recG, and recN mutations. The frequency of spontaneous and UV-induced illegitimate recombination in the uvrD recR double mutant was comparable to that of the uvrD single mutant, suggesting that UvrD works at the same step as RecR in the RecET-mediated recombination pathway. Nucleotide sequence analyses of the recombination junctions showed that RecET-mediated illegitimate recombination detected in UvrD-deficient strain is short-homology-dependent. Based on these and previous results, we propose a model for the role of UvrD on RecET-mediated illegitimate recombination.
authors.affiliation: Institute of Medical Science, Medinet Inc., Tokyo, Japan.
authors.forename: Kouya
authors.initials: K
authors.lastname: Shiraishi
authors.forename: Yukiho
authors.initials: Y
authors.lastname: Imai
authors.forename: Shinji
authors.initials: S
authors.lastname: Yoshizaki
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Tadaki
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ogata
authors.forename: Hideo
authors.initials: H
authors.lastname: Ikeda
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D004265:DNA Helicases / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000502:physiology*; D005090:Exodeoxyribonucleases / Q000502:physiology*; D008969:Molecular Sequence Data; D011995:Recombination, Genetic / Q000235:genetics / Q000502:physiology*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C084497:RecT protein, E coli; D005090:Exodeoxyribonucleases; C038225:recE protein, E coli; C506746:UvrD protein, E coli; D004265:DNA Helicases