title: Age-dependent decreases in mitogen-stimulation level and RNA content in peripheral blood mononuclear cells of Down syndrome patients.
abstract: OBJECTIVE
  The aim of the present study was to determine whether or not the phytohemagglutinin (PHA)-activated proliferation and average RNA content in peripheral blood mononuclear cells (PBMC) of Down syndrome (DS) patients change with age.
  
  
  METHODS
  Stimulated portion of PBMC and total RNA levels in these cells after 72 h of PHA stimulation from 38 DS patients were compared with 28 age-matched healthy controls using flow cytometric measurement.
  
  
  RESULTS
  Decreased ratio of PBMC from DS patients undergoes mitogenic stimulation with age (r = -0.84, P = 0.000). This decrease is not observed in the cells of control individuals (r = 0.03, P = 0.869). Stimulated PBMC in infants with DS have higher level of RNA contents compared to controls (Z = 2.227, P = 0.026). While RNA content in mitogen-stimulated PBMC of DS decreased progressively and significantly with age (r = -0.70, P = 0.000), no significant age-related change in RNA content was found among the cells of healthy individuals in the range of 0-27 year old (r = 0.275, P = 0.157, P > 0.05).
  
  
  CONCLUSIONS
  Age-dependent decreases in mitogen-activated proliferation ratio and average RNA content of PBMC from DS patients appear as regular events. These results may contribute to the explanation of the immune deficiency seen in DS patients since the PHA-stimulated cells are principally T-lymphocytes. This is the first report on the decrease in PHA-stimulated proliferation ratio (stimulability) and RNA level in PBMC of DS patients in relation to age.
authors.affiliation: Department of Medical Biology, Medical Faculty, Erciyes University, 38039 Kayseri, Turkey. zhamurcu@erciyes.edu.tr
authors.forename: Zuhal
authors.initials: Z
authors.lastname: Hamurcu
authors.forename: Halil
authors.initials: H
authors.lastname: Demirtas
authors.forename: Turkan
authors.initials: T
authors.lastname: Patiroglu
authors.forename: Sefer
authors.initials: S
authors.lastname: Kumandas
authors.forename: Nalan
authors.initials: N
authors.lastname: Imamoglu
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Ozkul
authors.forename: Hamiyet
authors.initials: H
authors.lastname: Donmez-Altuntas
mesh_terms: D000293:Adolescent; D000328:Adult; D000375:Aging; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004314:Down Syndrome / Q000473:pathology*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008297:Male; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D012313:RNA / Q000032:analysis*
chemical_list: D008934:Mitogens; D010835:Phytohemagglutinins; D012313:RNA