Giant panda (Ailuropoda melanoleuca) spermatozoon decondensation in vitro is not compromised by cryopreservation.
Natural breeding of giant pandas in captivity is compromised, making artificial insemination and spermatozoa cryopreservation essential for genetic management. This study examined the influence of freeze-thawing on traditional parameters such as motility and spermatozoon functionality, specifically decondensation in vitro. Giant panda spermatozoa were assessed before and after rapid cryopreservation (4 degrees C to -130 degrees C over 2 min) in liquid nitrogen vapour. Spermatozoa pre-incubated in medium for 6 h were co-incubated with cat zonae (2 zonae microL(-1)) for 30 min to effect capacitation and an acrosome reaction. Spermatozoa were then mixed with mature cat oocyte cytoplasm (2 cytoplasm microL(-1)) for 4 h and evaluated for decondensation. Frozen spermatozoa were less motile (P < 0.05) than fresh counterparts immediately post-thawing, but not after 6 h incubation. There were more (P < 0.05) spermatozoa with completely diffused chromatin post-thaw (10.4 +/- 1.3%; mean +/- s.e.m.) compared to fresh counterparts (5.1 +/- 1.0%). However, there was no overall difference (P > 0.05) in the incidence of decondensation between fresh (4 h, 69.8 +/- 5.9%) and thawed (4 h, 71.5 +/- 4.9%) spermatozoa after exposure to cat oocyte cytoplasm. It is concluded that the 'rapid' method now used to cryopreserve giant panda spermatozoa has little impact on spermatozoon decondensation.
Spindler
Rebecca E
RE
Conservation and Research Center, Smithsonian's National Zoological Park, Front Royal, VA 22630, USA. spindler@uoguelph.ca
Huang
Yan
Y
Howard
Jo Gayle
JG
Wang
Peng Yan
PY
Zhang
Hemin
H
Zhang
Guiquan
G
Wildt
David E
DE
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D008297:Male; D012662:Semen Preservation / Q000379:methods; D013094:Spermatozoa / Q000166:cytology*; D001503:Ursidae