Protective effect of taurine on respiratory burst activity of polymorphonuclear leukocytes in endotoxemia.
The aim of this study was to evaluate the effect of endotoxin on PMN leukocyte respiratory burst activity by measuring G6PD, NADPH oxidase and XO activities in guinea pig. In addition, the possible protective role of taurine against endotoxin-mediated PMN leukocyte function was examined. All experiments were performed with four groups (control, taurine, endotoxemia, taurine plus endotoxin) of ten guinea pigs. After the endotoxin was administrated (4 mg/kg) both G6PD and NADPH oxidase activities were significantly reduced compared with the control group. NADPH oxidase activity returned to the control value and G6PD activity also increased but it did not reach the control value. However when taurine was administrated (300 mg/kg) the activity of NADPH oxidase reached the control value; furthermore, G6PD activity also increased but it could not reach to the control value. When taurine was administrated alone, no effect on these enzymes was observed. Following the endotoxin administration, the activity of XO considerably increased. When taurine was administrated together with endotoxine and alone, this activity decreased compared to control value in both conditions. These results indicate that the O2*- formation in PMN leukocytes after the endotoxin administration is ensured by the catalysis of XO due to the inhibited NADPH oxidase activity. It was observed that taurine has considerable anti-inflammatory and antioxidant effects. However, conflicting results were obtained when taurine was administrated alone or together with an oxidant agent.
Ekremoğlu
M
M
Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey.
Türközkan
N
N
Erdamar
H
H
Kurt
Y
Y
Yaman
H
H
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D019446:Endotoxemia / Q000139:chemically induced / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D004731:Endotoxins / Q000633:toxicity; D006168:Guinea Pigs; D008297:Male; D009504:Neutrophils / Q000201:enzymology* / Q000473:pathology; D016877:Oxidants / Q000633:toxicity; D010088:Oxidoreductases / Q000378:metabolism*; D011897:Random Allocation; D016897:Respiratory Burst / Q000187:drug effects*; D013481:Superoxides / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology*
D000975:Antioxidants; D004731:Endotoxins; D016877:Oxidants; D013481:Superoxides; D013654:Taurine; D010088:Oxidoreductases