Effect of a conjugated quercetin metabolite, quercetin 3-glucuronide, on lipid hydroperoxide-dependent formation of reactive oxygen species in differentiated PC-12 cells.
To assess the efficacy of conjugated quercetin metabolites as attenuators for oxidative stress in the central nervous system, we measured the 13-hydroperoxyoctadecadienoic acid (13-HPODE)-dependent formation of reactive oxygen species (ROS) in pheochromocytoma PC-12 cells in the presence of quercetin 3-O-beta-glucuronide (Q3GA) and related compounds. A 2',7'-dichlorofluorescin (DCFH) assay showed that Q3GA significantly suppressed the formation of ROS, when it was coincubated with 13-HPODE (coincubation system). However, it was less effective than quercetin aglycon in the concentration range from 0.5 to 10 microM. In an experiment in which the cells were incubated with the test compounds for 24 h before being exposed to 13-HPODE, Q3GA was also effective in suppressing the formation of ROS in spite that little Q3GA was taken up into the cells. These results suggest that antioxidative metabolites of quercetin are capable of protecting nerve cells from attack of lipid hydroperoxides.
Shirai
Mutsuko
M
Department of Food Science, Institute for Health Biosciences, The University of Tokushima Graduate School, Kuramoto-cho 3, Tokushima 770-8503, Japan.
Kawai
Yoshichika
Y
Yamanishi
Rintaro
R
Kinoshita
Takashi
T
Chuman
Hiroshi
H
Terao
Junji
J
D000818:Animals; D002454:Cell Differentiation; D016166:Free Radical Scavengers / Q000378:metabolism / Q000494:pharmacology*; D008041:Linoleic Acids / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000633:toxicity; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000378:metabolism / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D011794:Quercetin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism
D016166:Free Radical Scavengers; D008041:Linoleic Acids; D008054:Lipid Peroxides; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C443401:quercetin 3-O-glucuronide; C038649:13-hydroperoxy-9,11-octadecadienoic acid; D011794:Quercetin