title: Evaluation of molecular-Beacon, TaqMan, and fluorescence resonance energy transfer probes for detection of antibiotic resistance-conferring single nucleotide polymorphisms in mixed Mycobacterium tuberculosis DNA extracts.
abstract: The ability of fluorescence resonance energy transfer, molecular-beacon, and TaqMan probes to detect single nucleotide polymorphism (SNP) in the presence of a wild-type allele was evaluated using drug resistance-conferring SNPs in mixed Mycobacterium tuberculosis DNA. It was found that both the absolute quantity and the ratio of alleles determine the detection sensitivity of the probe systems.
authors.affiliation: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, United Kingdom.
authors.forename: Hasan
authors.initials: H
authors.lastname: Yesilkaya
authors.forename: Francesca
authors.initials: F
authors.lastname: Meacci
authors.forename: Stefan
authors.initials: S
authors.lastname: Niemann
authors.forename: Doris
authors.initials: D
authors.lastname: Hillemann
authors.forename: Sabine
authors.initials: S
authors.lastname: Rüsch-Gerdes
authors.forename: Michael R
authors.initials: MR
authors.lastname: Barer
authors.forename: Peter W
authors.initials: PW
authors.lastname: Andrew
authors.forename: Marco R
authors.initials: MR
authors.lastname: Oggioni
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D015341:Nucleic Acid Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial; D015341:Nucleic Acid Probes