[Study on homogeneity of enzymatic degradation of chitosan as biomaterials by gel permeation chromatography].
Chitosan is an important biomedical material, and its degree of deacetylation is a main parameter of its biodegradation. Gel permeation chromatography was used to investigate the lysozymic degradation of two types of chitosan samples (A and B) with similar degree of deacetylation and relative molecular mass but with different distributions of two units of N-acetyl-D-glucosamine and D-glucosamine. Weight average relative molecular mass, polydispersity and gel permeation chromatograms during the degradation process were obtained. It was found that chitosan sample A with random distribution of the two units underwent a homogeneous degradation process while chitosan sample B with block distribution underwent a heterogeneous degradation process. The results suggest that the homogeneity of the degradation of chitosan materials by lysozyme depends on the distribution type of the two units, which can help to design chitosan-based biomedical devices.
Ren
Dongwen
D
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. rendw@dicp.ac.cn
Yi
Hongfu
H
Xie
Weiyang
W
Ma
Xiaojun
X
D000117:Acetylglucosamine / Q000737:chemistry; D000118:Acetylglucosaminidase / Q000378:metabolism*; D001672:Biocompatible Materials / Q000378:metabolism; D001673:Biodegradation, Environmental; D018929:Cell Culture Techniques; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D002850:Chromatography, Gel / Q000379:methods*; D019411:Clinical Laboratory Techniques; D007700:Kinetics; D008422:Materials Testing / Q000592:standards; D008970:Molecular Weight; D009113:Muramidase / Q000378:metabolism*; D013696:Temperature
D001672:Biocompatible Materials; D048271:Chitosan; D009113:Muramidase; D000118:Acetylglucosaminidase; D000117:Acetylglucosamine