Inhibitory effects of sulfamic acid on three thiosulfate-oxidizing chemolithotrophs.
Sulfamate is an analogue of thiosulfate, and the sodium and potassium salts of sulfamic acid inhibited the chemolithoautotrophic growth on thiosulfate of Acidithiobacillus ferrooxidans and Halothiobacillus neapolitanus. The chemo-organotrophic growth of Paracoccus versutus on sucrose was similarly inhibited by sulfamate. Thiosulfate oxidation by suspensions of H. neapolitanus was, however, unaffected by sulfamate, showing that sulfamate did not directly affect thiosulfate uptake, activation or oxidation. Inhibition of P. versutus was not relieved by cysteine and methionine, indicating that sulfate uptake and sulfur amino acid biosynthesis were not directly affected by sulfamate. Sulfamate was not degraded by any of the bacteria, and so could not serve as an alternative to thiosulfate as an energy-yielding substrate. Sulfamate is also an analogue of ammonia and might act like hydrazine by inhibiting ammonium uptake or an essential enzyme activity.
Lusty
James R
JR
University of Wales, Newport, Caerleon Campus, Newport, South Wales, UK.
Hughes
Martin N
MN
Kelly
Donovan P
DP
D042763:Acidithiobacillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D052837:Chemoautotrophic Growth / Q000187:drug effects; D003470:Culture Media; D003545:Cysteine / Q000494:pharmacology; D044222:Halothiobacillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008715:Methionine / Q000494:pharmacology; D010084:Oxidation-Reduction; D010230:Paracoccus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D012492:Salts / Q000378:metabolism / Q000494:pharmacology; D013395:Sucrose / Q000378:metabolism; D013451:Sulfonic Acids / Q000378:metabolism / Q000494:pharmacology*; D013885:Thiosulfates / Q000378:metabolism
D000900:Anti-Bacterial Agents; D003470:Culture Media; D012492:Salts; D013451:Sulfonic Acids; D013885:Thiosulfates; D013395:Sucrose; C005741:sulfamic acid; D008715:Methionine; D003545:Cysteine