Adsorption of human IgG on to poly(N-isopropylacrylamide)-based polymer particles.
Thermosensitive poly(N-isopropylacrylamide)-based polymer particles were synthesised, and screened for the adsorption of human immunoglobulin G (hIgG). At pH 9 the adsorption on microgel particles was strongly affected by temperature, approximately 40 mg hIgG/g support (90% of initial hIgG) being adsorbed at 40 degrees C but only 10% of initial hIgG at 25 degrees C. At pH 5 the maximum adsorbed amount (20 mg hIgG/g support) was similar for both temperatures. The adsorption of hIgG on to charged poly(methyl methacrylate)/poly(N-isopropylacrylamide) core-shell latexes was negligible (5-10 mg hIgG/g support) at the same temperature and pH conditions. The lower adsorption of hIgG onto the core-shell particles is explained by steric interactions due to the small size of the shell.
Silva
Claudia S O
CS
Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av Rovisco Pais, 1049-001, Lisbon, Portugal.
Baptista
Ricardo P
RP
Santos
Andrea M
AM
Martinho
José M G
JM
Cabral
Joaquim M S
JM
Taipa
M Angela
MA
D000178:Acrylamides / Q000493:pharmacokinetics*; D000180:Acrylic Resins; D000327:Adsorption; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry* / Q000493:pharmacokinetics; D013696:Temperature; D013997:Time Factors
D000178:Acrylamides; D000180:Acrylic Resins; D007074:Immunoglobulin G; D011108:Polymers; C052970:poly-N-isopropylacrylamide