Transgenic rice plants expressing trichothecene 3-O-acetyltransferase show resistance to the Fusarium phytotoxin deoxynivalenol.
Fusarium head blight (FHB) is a devastating disease of small grain cereal crops caused by the necrotrophic pathogen Fusarium graminearum and Fusarium culmorum. These fungi produce the trichothecene mycotoxin deoxynivalenol (DON) and its derivatives, which enhance the disease development during their interactions with host plants. For the self-protection, the trichothecene producer Fusarium species have Tri101 encoding trichothecene 3-O-acetyltransferase. Although transgenic expression of Tri101 significantly reduced inhibitory action of DON on tobacco plants, there are several conflicting observations regarding the phytotoxicity of 3-acetyldeoxynivalenol (3-ADON) to cereal plants; 3-ADON was reported to be highly phytotoxic to wheat at low concentrations. To examine whether cereal plants show sufficient resistance to 3-ADON, we generated transgenic rice plants with stable expression and inheritance of Tri101. While root growth of wild-type rice plants was severely inhibited by DON in the medium, this fungal toxin was not phytotoxic to the transgenic lines that showed trichothecene 3-O-acetylation activity. This is the first report demonstrating the DON acetylase activity and DON-resistant phenotype of cereal plants expressing the fungal gene.
Ohsato
Shuichi
S
Plant & Microbial Metabolic Engineering Research Unit and Laboratory for Remediation Research, Discovery Research Institute and Plant Science Center, RIKEN, 2-1 Hirosawa, Wako, Saitama, Japan.
Ochiai-Fukuda
Tetsuko
T
Nishiuchi
Takumi
T
Takahashi-Ando
Naoko
N
Koizumi
Shinzo
S
Hamamoto
Hiroshi
H
Kudo
Toshiaki
T
Yamaguchi
Isamu
I
Kimura
Makoto
M
D000107:Acetylation; D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D004351:Drug Resistance / Q000235:genetics*; D005670:Fusarium / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D005822:Genetic Vectors / Q000235:genetics; D015394:Molecular Structure; D009183:Mycotoxins / Q000378:metabolism; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic; D014255:Trichothecenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
D009183:Mycotoxins; D014255:Trichothecenes; C038972:acetyldeoxynivalenol; D000123:Acetyltransferases; C110205:trichothecene 3-O-acetyltransferase