[Sequence variation of chloroplast gene infA-rpl36 region occurred in some Triticeae species].
Based on the sequenced wheat chloroplast genome (cpDNA), a pair of primers in infA-rp136 region was designed and used to amplify DNA from 12 diploid or polyploid Triticeae species. The 12 PCR products were cloned and sequenced. The resulting sequences ranged from 584 to 601 bp. DNA sequence analysis revealed that variation was higher in their intergenic regions than in their coding regions. Among these 12 species, the DNA sequence of coding region of infA gene showed homology as high as 97 percent, indicating that the infA gene is highly conserved among species. However, substantial deletions and insertions were found in 5 out of 12 deduced amino acid sequences, confirming that infA is one of the most evolutionally active cpDNA genes. Whereas the low variation was observed in rp136 gene, implying that the different genes has different evolutionary speed. The constructed phylogenetic trees demonstrate that the polyploidy species Thinopyrum intermedium might have different origin of cytoplasm, and their cytoplasm origins are as complex as their nuclear genome origins.
Liu
Chang
C
School of Life Science and Technology, University of Electronic Science and Technology of China. liuchang639@163.com
Yang
Zu-Jun
ZJ
Li
Guang-Rong
GR
Feng
Juan
J
Deng
Ke-Jun
KJ
Huang
Jian
J
Ren
Zheng-Long
ZL
D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts / Q000235:genetics*; D003001:Cloning, Molecular; D015977:Eukaryotic Initiation Factor-1 / Q000737:chemistry / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D006109:Poaceae / Q000166:cytology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*
D015977:Eukaryotic Initiation Factor-1; D010940:Plant Proteins; D012269:Ribosomal Proteins