title: Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae.
abstract: A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohkubo
authors.forename: Hideaki
authors.initials: H
authors.lastname: Miyashita
authors.forename: Akio
authors.initials: A
authors.lastname: Murakami
authors.forename: Haruko
authors.initials: H
authors.lastname: Takeyama
authors.forename: Tohru
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Mamoru
authors.initials: M
authors.lastname: Mimuro
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D000458:Cyanobacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D056890:Eukaryota / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002734:Chlorophyll; C107509:chlorophyll d