Helicobacter pylori infection can modulate the susceptibility of gastric mucosa cells to MNNG.
The pathogenesis of stomach cells can be associated with their susceptibility to exogenous dietary irritants, like nitrosamines such as dimethylnitrosamines (DMNA), and to the effects of non-dietary factors, including Helicobacter pylori infection. We used N-methyl-N'-nitro N-nitrosoguanidyne (MNNG) as a surrogate agent that induces a spectrum of DNA damage similar to DMNA. Using the alkaline comet assay, we showed that antioxidants--vitamins C and E, quercetin, and melatonin--reduced the genotoxic effect of MNNG in H. pylori-infected and non-infected human gastric mucosa cells (GMCs). To compare the sensitivity of the stomach and the blood, the experiment was also carried out in peripheral blood. We observed a higher level of DNA damage induced by MNNG in H. pylori-infected than in noninfected GMCs. We did not note any difference in the efficacy of the repair of the damage in either type of GMC. H. pylori infection may play an important role in the pathogenesis of GMCs, as it can modulate their susceptibility to dietary mutagens/carcinogens, thus contributing to gastric cancer.
Arabski
Michał
M
Department of Molecular Genetics, University of Łódź, Łódź, Poland.
Kazmierczak
Paweł
P
Wiśniewska-Jarosińska
Maria
M
Morawiec
Zbigniew
Z
Morawiec-Bajda
Alina
A
Klupińska
Grazyna
G
Drzewoski
Józef
J
Chojnacki
Jan
J
Błasiak
Janusz
J
D000328:Adult; D000368:Aged; D002273:Carcinogens / Q000494:pharmacology*; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology*; D020022:Genetic Predisposition to Disease; D016481:Helicobacter Infections / Q000235:genetics / Q000378:metabolism*; D016480:Helicobacter pylori / Q000187:drug effects; D006801:Humans; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D008875:Middle Aged; D009153:Mutagens / Q000494:pharmacology*
D002273:Carcinogens; D009153:Mutagens; D008769:Methylnitronitrosoguanidine