[Comparison of the inhibitory activities of salvianolic acid B and Ginkgo biloba extract EGb 761 on neurotoxicity of beta-amyloid peptide].
OBJECTIVE
To compare the effects of salvianolic acid B (Sal B) and Ginkgo biloba extract EGb 761 on beta-amyloid peptide (beta-AP) fibril formation and cytotoxicity to PC12 cells.


METHODS
The inhibitory effects of Sal B and EGb 761 on beta-AP1-40 fibril formation were determined by using fluorescence analysis with Thioflavin T (ThT) and electron microscopic image. beta-AP25-35 was aged by incubating at 37 degrees C for 7 d, then the protein was incubated with PC12 cells. The protective effects of Sal B and EGb 761 against cytotoxicity induced by aged beta-AP25-35 in PC12 cells were evaluated by MTT reduction assay and flow cytometric analysis. beta-AP25-35-induced accumulation of intracellular reactive oxygen species (ROS) was determined by fluorescence analysis.


RESULTS
Both Sal B and EGb 761 inhibited the formation of amyloid fibrils, protected PC12 cells from beta-AP25-35-induced cytotoxicity, and decreased ROS accumulation caused by beta-AP25-35. The effective doses of Sal B were far lower than those of EGb 761.


CONCLUSIONS
Sal B was much more efficient than EGb 761 in inhibiting beta-AP aggregation and in protecting PC12 cells from beta-AP-induced cytotoxicity.
Liu
Chang-suo
CS
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Hu
Jin-feng
JF
Chen
Nai-hong
NH
Zhang
Jun-tian
JT
D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D005434:Flow Cytometry; D020441:Ginkgo biloba / Q000737:chemistry; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism; D008854:Microscopy, Electron; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000737:chemistry; D000096983:Ginkgo Extract; D053630:Depsides
D016229:Amyloid beta-Peptides; D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C074636:amyloid beta-protein (25-35); C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract; C076944:salvianolic acid B; D053630:Depsides