Association and insulin regulated translocation of hormone-sensitive lipase with PTRF.
Polymerase I and transcript release factor (PTRF) is in human adipocytes mainly localized at the plasma membrane. This localization was under control of insulin, which translocated PTRF to the cytosol and nucleus, indicating a novel role for PTRF in insulin transcriptional control. In the plasma membrane PTRF was specifically bound to a triacylglycerol-metabolizing subclass of caveolae containing hormone-sensitive lipase (HSL). In response to insulin PTRF was translocated to the cytosol in parallel with HSL. PTRF and HSL were quantitatively immunoprecipitated from the cytosol by antibodies against either PTRF or HSL. The findings indicate also a novel extranuclear function for PTRF in the control of lipolysis.
Aboulaich
Nabila
N
Department of Cell Biology and Diabetes Research Centre, Linköping University, SE58185 Linköping, Sweden.
Ortegren
Unn
U
Vener
Alexander V
AV
Strålfors
Peter
P
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007328:Insulin / Q000008:administration & dosage*; D008066:Lipolysis / Q000187:drug effects / Q000502:physiology*; D021381:Protein Transport / Q000187:drug effects / Q000502:physiology*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D002787:Sterol Esterase / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects*
C497835:CAVIN1 protein, human; D007328:Insulin; D016601:RNA-Binding Proteins; D002787:Sterol Esterase