Intelligent fluorescent nucleoside in sensing cytosine base: importance of hydrophobic nature of perylene fluorophore.
Fluorescence response upon hybridization of perylene labeled oligonucleotide probes depends on the microenvironment experienced by the perylene fluorophore. In mismatched duplex ((Per)U-C), enhanced fluorescence was observed while in matched duplex ((Per)U-A) fluorescence intensity decreased considerably. This observation will be a promising research effort in giving rise to a new powerful tool in detection of SNP.
Bag
Subhendu Sekhar
SS
NEWCAT Institute, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan.
Saito
Yoshio
Y
Hanawa
Kazuo
K
Kodate
Satoshi
S
Suzuka
Isamu
I
Saito
Isao
I
D001483:Base Sequence; D001665:Binding Sites; D003596:Cytosine / Q000032:analysis*; D005456:Fluorescent Dyes; D009711:Nucleotides; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010569:Perylene; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D009711:Nucleotides; D009838:Oligodeoxyribonucleotides; D010569:Perylene; D003596:Cytosine