The sigma(E) cell envelope stress response of Streptomyces coelicolor is influenced by a novel lipoprotein, CseA.
We have investigated the role of CseA in the sigma(E) cell envelope stress response of the gram-positive bacterium Streptomyces coelicolor. sigma(E) is an extracytoplasmic function RNA polymerase sigma factor required for normal cell envelope integrity in S. coelicolor. sigma(E) is encoded within a four-gene operon that also encodes CseA, a protein of unknown function, CseB, a response regulator and CseC, a transmembrane sensor histidine kinase (Cse represents control of sigma E). Previous work has shown that transcription of the sigE gene is completely dependent on the CseBC two-component system and that the CseBC-sigma(E) signal transduction system is induced by a wide variety of cell-wall-damaging agents. Here we address the role of CseA, a protein with no homologues outside the streptomycetes. We show that CseA is a novel lipoprotein localized to the extracytoplasmic face of the cell membrane and that loss of CseA results in upregulation of the sigE promoter.
Hutchings
Matthew I
MI
Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom. m.hutchings@uea.ac.uk
Hong
Hee-Jeon
HJ
Leibovitz
Emmanuelle
E
Sutcliffe
Iain C
IC
Buttner
Mark J
MJ
D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D015151:Immunoblotting; D008074:Lipoproteins / Q000235:genetics / Q000502:physiology*; D008958:Models, Molecular; D009876:Operon / Q000235:genetics; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology*; D048372:Streptomyces coelicolor / Q000235:genetics / Q000502:physiology*; D013347:Subcellular Fractions / Q000737:chemistry; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins; C120882:CseB protein, Streptomyces coelicolor; D008074:Lipoproteins; D012808:Sigma Factor; D014157:Transcription Factors; C063202:sporulation-specific sigma factors