The cloning, crystallization and preliminary X-ray analysis of XC2113, a YaeQ protein from Xanthomonas campestris.
Xanthomonas campestris is a Gram-negative bacterium that is phytopathogenic to cruciferous plants and causes worldwide agricultural loss. It is therefore important to identify potential pathogenic factors involved in this plant disease. Here, the cloning, expression, crystallization and preliminary X-ray analysis of XC2113, a YaeQ protein possibly involved in the production of virulence factors in Xanthomonas campestris pathovar campestris, are reported. The XC2113 crystals diffracted well to a resolution of at least 1.28 A. They are orthorhombic and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 32.86, b = 62.69, c = 79.96 A.
Chio
Kuo-Cheng
KC
Institute of Biochemistry, National Chung-Hsing University, Taichung 40227, Taiwan.
Chin
Ko-Hsin
KH
Gao
Fei Philip
FP
Lyu
Ping-Chiang
PC
Shr
Hui-Lin
HL
Wang
Andrew H-J
AH
Chou
Shan-Ho
SH
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D016959:Xanthomonas campestris / Q000737:chemistry*
D001426:Bacterial Proteins