title: Proteomics analysis reveals insight into the mechanism of H-Ras-mediated transformation.
abstract: We implemented a proteomics approach to the systematical analysis of the alterations in the proteome of NIH3T3 cells transformed by oncogenic H-RasV12. Forty-four proteins associated with Ras-mediated transformation have been identified, and 28 proteins were not previously reported. RT-PCR analysis showed that approximately 44% of target proteins identified showed concomitant changes in mRNA abundance. A principal finding was the up-regulation of gankyrin, which was the first evidence to show that gankyrin pathway was implicated in Ras-activated transformation.
authors.affiliation: Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing 100850, China.
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Jin
authors.forename: Kun
authors.initials: K
authors.lastname: He
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Bai
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Hui-Yan
authors.initials: HY
authors.lastname: Li
authors.forename: Jiang-Hong
authors.initials: JH
authors.lastname: Man
authors.forename: Bing-Yu
authors.initials: BY
authors.lastname: Liu
authors.forename: Wei-Li
authors.initials: WL
authors.lastname: Gong
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Li
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D016000:Cluster Analysis; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D015689:Oncogene Protein p21(ras) / Q000235:genetics*; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D040901:Proteomics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D020543:Proteome; D012333:RNA, Messenger; D015689:Oncogene Protein p21(ras)