[Effect of lenti-mCCL20 on the growth of mouse tumor].
OBJECTIVE
We aim at inducing a potent antitumor immune response via CCL20 expressing in situ tumor.


METHODS
We constructed a recombinant lentivirus encoding mouse CCL20 cDNA and transduced the mouse mesenchymal stem cells (mMSCs) in vitro, and then selected the transfected cells to get a stable mixed mCCL20-expressing pool (named as lenti-mCCL20-mMSCs) with blasticidin. By the same means, we produced another pool named as lenti-null-mMSCs. The CT26 was mixed with lenti-mCCL20-mMSC and inoculated sc into the left hinder back of BALB/c mouse. And also the CT26 was alone, or mixed with lenti-null-mMSCs or parent mMSCs, and then inoculated sc into BALB/c mice serving as controls.


RESULTS
We got a lenti-mCCL20-mMSCs expression construct. It was shown that mCCL20 increased intratumoral lymphocytes infiltration and facilitated tumor growth in syngeneic murine tumor model.


CONCLUSIONS
CCL20 expressing in situ tumor enhances intratumoral lymphocytes infiltration but facilitates tumor growth. However, the mechanism involved remains to be further elucidated.
Li
Hong-xia
HX
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
Chen
Ping
P
Wang
Lian
L
Xu
Jian-rong
JR
Wang
Guo-qing
GQ
Jia
Yong-qian
YQ
Yang
Li
L
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D054418:Chemokine CCL20; D019742:Chemokines, CC / Q000096:biosynthesis* / Q000235:genetics; D003110:Colonic Neoplasms / Q000276:immunology / Q000473:pathology*; D005260:Female; D016086:Lentivirus / Q000235:genetics* / Q000378:metabolism; D008214:Lymphocytes / Q000473:pathology*; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis* / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D011995:Recombination, Genetic; D014162:Transfection
C108355:CCL20 protein, human; D054418:Chemokine CCL20; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins