title: Analysis of 2-aminoacetophenone in wine using a stable isotope dilution assay and multidimensional gas chromatography-mass spectrometry.
abstract: The key compound in the wine off-flavor phenomenon "Untypische Alterungsnote" (UTA) is 2-aminoacetophenone (AAP). A new method for its quantitative analysis in wine is described. The analysis is based on solid phase extraction with LiChrolut EN as sorbent material followed by multidimensional gas chromatography coupled to mass spectrometry (MDGC-MS). Matrix effects due to the complex wine aroma composition could be overcome by an alkaline washing step of the extract and the use of selected ion monitoring as detection mode. Quantification is based on a stable isotope dilution assay using deuterated 2-aminoacetophenone as internal standard.
authors.affiliation: Dienstleistungszentrum Ländlicher Raum Rheinpfalz, Breitenweg 71, D-67435 Neustadt an der Weinstrasse, Germany. hans-georg.schmarr@dlr.rlp.de
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Schmarr
authors.forename: Sebastian
authors.initials: S
authors.lastname: Ganss
authors.forename: Wei
authors.initials: W
authors.lastname: Sang
authors.forename: Theodoros
authors.initials: T
authors.lastname: Potouridis
mesh_terms: D000098:Acetophenones / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D007201:Indicator Dilution Techniques; D007554:Isotopes; D015394:Molecular Structure; D015203:Reproducibility of Results; D014920:Wine / Q000032:analysis*
chemical_list: D000098:Acetophenones; D007554:Isotopes; C055495:2-aminoacetophenone