title: CHD6 is a DNA-dependent ATPase and localizes at nuclear sites of mRNA synthesis.
abstract: The CHD family of proteins comprises ATP-dependent chromatin remodeling enzymes, which combine chromodomains, with SWI2/SNF2 ATPase/helicase motifs and DNA-binding capability. In the last few years, CHD proteins have drawn increased attention, because some of them were found to form large multi-subunit complexes, involved in transcription-related events like gene activation, suppression, or histone modification. We previously described the identification of CHD6, a protein of the CHD subfamily III. In the present study, we report that CHD6 is expressed in cells of human origin and in various mouse tissues. Subcellular distribution of CHD6 is restricted to the nucleoplasm. We further show that CHD6 colocalizes with both hypo- and hyper-phosphorlylated forms of RNA polymerase II. CHD6 was found to be present at sites of mRNA synthesis and to be part of a high molecular weight complex. Moreover, we demonstrate DNA-dependent ATPase activity of CHD6.
authors.affiliation: Centro Nacional de Biotecnología (CNB), Cantoblanco, 28049 Madrid, Spain. tlutz@cnb.uam.es
authors.forename: Thomas
authors.initials: T
authors.lastname: Lutz
authors.forename: Reinhard
authors.initials: R
authors.lastname: Stöger
authors.forename: Amelia
authors.initials: A
authors.lastname: Nieto
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D008970:Molecular Weight; D046912:Multiprotein Complexes; D010766:Phosphorylation; D012319:RNA Polymerase II / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D012333:RNA, Messenger; D004247:DNA; D012319:RNA Polymerase II; D000251:Adenosine Triphosphatases; D004265:DNA Helicases