title: High frequency early in vitro flowering of Dendrobium Madame Thong-In (Orchidaceae).
abstract: We have successfully developed a method to induce early in vitro flowering of the self-pollinated seedlings of a tropical orchid hybrid, Dendrobium Madame Thong-In. Transition of vegetative shoot apical meristem to inflorescence meristem was observed when young protocorms were cultured in modified KC liquid medium. In contrast, protocorms cultured on Gelrite-solidified medium only produced axillary shoots and roots. CW was required to trigger the transitional shoot apical meristem and BA enhanced inflorescence stalk initiation and flower bud formation. However, normal flower development was deformed in liquid medium but developed fully upon transferring to two-layered (liquid over Gelrite-solidified) medium. Under optimal condition, in vitro flowering was observed about 5 months after seed sowing. Segregation of flower colours was observed in these seedlings and seedpods formed upon artificial pollination of the in vitro flowers.
authors.affiliation: Department of Biological Sciences, National University of Singapore, Science Drive 4, 117543, Singapore, Singapore.
authors.forename: Guek Eng
authors.initials: GE
authors.lastname: Sim
authors.forename: Chiang Shiong
authors.initials: CS
authors.lastname: Loh
authors.forename: Chong Jin
authors.initials: CJ
authors.lastname: Goh
mesh_terms: D001593:Benzyl Compounds; D003059:Cocos / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D031667:Dendrobium / Q000187:drug effects / Q000254:growth & development*; D035264:Flowers / Q000187:drug effects / Q000254:growth & development*; D007701:Kinetin / Q000494:pharmacology; D018519:Meristem / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D011687:Purines; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D046509:Tissue Culture Techniques
chemical_list: D001593:Benzyl Compounds; D003470:Culture Media; D011687:Purines; C480551:benzylaminopurine; D007701:Kinetin