[Antitumor effects of nobiletin on Heps and its mechanism].
OBJECTIVE
To study the inhibitory effect and mechanism of nobiletin on Heps tumor bearing mice.


METHODS
Models of Heps tumor bearing mice were established. The inhibitory rates of tumor growth were calculated, the apoptosis morphology of tumor tissue was observed. The T lymphocyte transformation capacity was tested by MTT assay, the TNFalpha and IL-2 production were measured by LDH kits.


RESULTS
Nobiletin could significantly inhibit Heps tumor growth. The inhibitory rates were 42.14% - 65.09% (P < 0.01). The morphology of tumor tissues in nobiletin group had typical characters of necrosis and apoptosis through transmission electron microscope. Nobiletin could stimulate T lymphocyte transformation and the production of TNFalpha and IL-2.


CONCLUSIONS
Nobiletin has obvious antitumor effect on Heps, the main mechanism is to enhance the cellular immune function and induce apoptosis of tumor tissue.
Zhang
Hong-quan
HQ
The Medical and Pharmaceutical Academy, Yangzhou University, Yangzhou 225001, China. lixin2001@sohu.com
Ge
Hui
H
Cheng
Mei-ying
MY
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002957:Citrus / Q000737:chemistry; D005260:Female; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000517:prevention & control* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010946:Plants, Medicinal / Q000737:chemistry; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000972:Antineoplastic Agents, Phytogenic; D047309:Flavones; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; C008661:nobiletin