Full-length genome sequences of two SARS-like coronaviruses in horseshoe bats and genetic variation analysis.
Bats were recently identified as natural reservoirs of SARS-like coronavirus (SL-CoV) or SARS coronavirus-like virus. These viruses, together with SARS coronaviruses (SARS-CoV) isolated from human and palm civet, form a distinctive cluster within the group 2 coronaviruses of the genus Coronavirus, tentatively named group 2b (G2b). In this study, complete genome sequences of two additional group 2b coronaviruses (G2b-CoVs) were determined from horseshoe bat Rhinolophus ferrumequinum (G2b-CoV Rf1) and Rhinolophus macrotis (G2b-CoV Rm1). The bat G2b-CoV isolates have an identical genome organization and share an overall genome sequence identity of 88-92 % among themselves and between them and the human/civet isolates. The most variable regions are located in the genes encoding nsp3, ORF3a, spike protein and ORF8 when bat and human/civet G2b-CoV isolates are compared. Genetic analysis demonstrated that a diverse G2b-CoV population exists in the bat habitat and has evolved from a common ancestor of SARS-CoV.
Ren
Wuze
W
Graduate School of CAS, Beijing 100039, China.|State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences (CAS), Wuhan, Hubei 430071, China.
Li
Wendong
W
Institute of Zoology, CAS, Beijing 100080, China.|Graduate School of CAS, Beijing 100039, China.
Yu
Meng
M
CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, VIC 3220, Australia.
Hao
Pei
P
Shanghai Center for Bioinformation Technology, Shanghai 200235, China.
Zhang
Yuan
Y
School of Life Sciences, Fudan University, Shanghai 200433, China.
Zhou
Peng
P
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences (CAS), Wuhan, Hubei 430071, China.
Zhang
Shuyi
S
Institute of Zoology, CAS, Beijing 100080, China.
Zhao
Guoping
G
Shanghai Center for Bioinformation Technology, Shanghai 200235, China.
Zhong
Yang
Y
School of Life Sciences, Fudan University, Shanghai 200433, China.
Wang
Shengyue
S
School of Life Sciences, Fudan University, Shanghai 200433, China.|Shanghai Center for Bioinformation Technology, Shanghai 200235, China.
Wang
Lin-Fa
LF
CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, VIC 3220, Australia.
Shi
Zhengli
Z
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences (CAS), Wuhan, Hubei 430071, China.
D000818:Animals; D002681:China; D002685:Chiroptera / Q000821:virology*; D014644:Genetic Variation; D016679:Genome, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D045169:Severe Acute Respiratory Syndrome / Q000821:virology; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D045949:Viverridae / Q000821:virology
D006389:Hemagglutinins, Viral; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C488151:sars7a protein, SARS virus; C578557:spike glycoprotein, SARS-CoV; C510959:Nsp3 protein, SARS-CoV; D012324:RNA-Dependent RNA Polymerase