title: [Study on vitrification of porcine embryos by open pulled straw method].
abstract: 291 embryos (Blastocyst/Morula) from 20 donor sows were vitrified by two step method with OPS (open pulled straw) in solution I (TCM199 + 20% FBS + 10% EG + 10% DMSO) for 3min, and solution II (TCM199 + 20% FBS + 20% EG + 20% DMSO + 0.4mol/L SUC) for 1min, stored in liquid nitrogen for 3 months, and transferred into 8 recipient sows after warming, one recipient sow was pregnant and 8 alive piglets were born. This is the first paper to report getting alive piglets by vitrification in China.
authors.affiliation: Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, China. zhangdefu10@yahoo.com.cn
authors.forename: De-Fu
authors.initials: DF
authors.lastname: Zhang
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Hua-Li
authors.initials: HL
authors.lastname: Wu
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Zheng
authors.forename: Zhao-Kai
authors.initials: ZK
authors.lastname: Wang
authors.forename: Shao-Bing
authors.initials: SB
authors.lastname: Wang
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary*; D004624:Embryo Transfer; D005260:Female; D011247:Pregnancy; D013552:Swine / Q000196:embryology*