title: Influence of resin composite shade and location of the gingival margin on the microleakage of posterior restorations.
abstract: OBJECTIVE
  The objective of this in vitro study was to evaluate the influence of resin composite shade and location of the gingival margin (enamel or dentin) on the microleakage of proximal restorations on posterior teeth.
  
  
  METHODS
  Sixty freshly extracted human third molars were prepared with standardized Class II box-shaped cavities with proportional size and shape, with distal gingival margins located on the enamel and mesial gingival margins on dentin. The teeth were randomly divided into 6 groups according to resin shade (n=10): G1-Incisal; G2-A1; G3-A2; G4-A3; G5-A3.5; G6-A4. The cavities were restored with a total-etch 1-bottle adhesive system and microhybrid resin composites inserted in 4 increments, light cured for 20 seconds through the occlusal surface, then an additional 60 seconds for each surface. After 1 week of immersion in distilled water, the specimens were thermocycled (500 cycles, 5 degrees -55 degrees C, 30 seconds dwell time), sealed with nail polish and immersed in 0.5% basic fuschin solution for 24 hours. The restorations were sectioned longitudinally, and microleakage was evaluated using a 0-3 score scale.
  
  
  RESULTS
  Data were subjected to Kruskal-Wallis and Wilcoxon tests at p<0.05. No statistically significant differences between groups were observed regarding the shade of resin composite (p=0.8570). When margins (enamel or dentin) were considered separately, statistically significant differences were observed between groups (p<0.0001), with enamel margins exhibiting lower degrees of microleakage.
  
  
  CONCLUSIONS
  The variation of resin composite shades utilized in this study did not influence the microleakage of Class II restorations. However, the location of the gingival margin influenced the microleakage.
authors.affiliation: Operative Dentistry, Federal University of Santa Catarina, Santa Catarina, Brazil. araujo.fabiano@ig.com.br
authors.forename: Fabiano de Oliveira
authors.initials: Fde O
authors.lastname: Araujo
authors.forename: Luiz Clovis Cardoso
authors.initials: LC
authors.lastname: Vieira
authors.forename: Sylvio
authors.initials: S
authors.lastname: Monteiro Junior
mesh_terms: D000134:Acid Etching, Dental; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D003116:Color; D004396:Coloring Agents; D003188:Composite Resins / Q000737:chemistry*; D001840:Dental Bonding; D003737:Dental Cavity Preparation / Q000145:classification; D003743:Dental Enamel / Q000648:ultrastructure*; D003763:Dental Leakage / Q000145:classification*; D003793:Dental Restoration, Permanent / Q000145:classification / Q000379:methods*; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008964:Molar, Third; D012394:Rosaniline Dyes; D013499:Surface Properties
chemical_list: D004396:Coloring Agents; D003188:Composite Resins; D017220:Dentin-Bonding Agents; C444701:Filtek Z250; D012394:Rosaniline Dyes; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; C025485:basic fuchsin