An evaluation of the effect of a gastric ischemia-reperfusion model with laser Doppler blood perfusion imaging.
The objectives of this study are to establish a gastric ischemia-reperfusion model and test it using the laser Doppler blood perfusion imaging (LDPI) method and to evaluate the role of the LDPI technique in the measure of gastric blood circulation. The right gastric artery of the rat was clamped for 30 min and then reperfused. The LDPI was used to display the blood circulation of the whole gastric surface during this process. The blood flow perfusion image of the gastric surface was displayed clearly. After the right gastric artery was clamped, the blood flow perfusion on the gastric surface decreased very significantly until the clamp was loosened. Following reperfusion, the blood flow suddenly increased. Within the first 10 min, the blood-flow perfusion exceeded the level before the clamping and then gradually became smooth and steady. The right gastric artery is a main pathway for gastric blood supply. LDPI can display successfully the blood circulation state of the stomach and the course of ischemia-reperfusion of a large area with an image.
Zhang
Dong
D
Department of Biomedical Engineering, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, 100700, China. zhangdongprof@yahoo.com.cn
Li
Shunyue
S
Wang
Shuyou
S
Ma
Huimin
H
D000818:Animals; D001158:Arteries / Q000502:physiology; D005260:Female; D017078:Laser-Doppler Flowmetry / Q000379:methods; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology*; D013270:Stomach / Q000098:blood supply*