Increases in plasma carotenoid concentrations in response to a major dietary change in the women's healthy eating and living study.
BACKGROUND
Cohort studies suggest that higher circulating carotenoid concentrations through food sources may reduce breast cancer events. Other intervention studies have not achieved the level of change in circulating carotenoids required to properly test this hypothesis.


METHODS
In a randomized trial of 2,922 breast cancer survivors, we examined blood and self-reported diet at baseline and 1 year. Intensive telephone counseling encouraged a plant-based diet in the intervention group. Diet was measured via 24-hour recalls, and a panel of plasma carotenoid concentrations was assessed at both time points.


RESULTS
The study intervention was associated with a 51% increase in total carotenoid concentration, from 2.272 +/- 1.294 to 3.440 +/- 2.320 micromol/L, achieved mainly by marked increases in targeted carotenoids: alpha-carotene, beta-carotene, and lutein. For each of these targeted carotenoids, the proportion of the intervention sample remaining below the cutpoint for the lowest baseline quartile decreased by one third to one half. After 1 year of study, half of the intervention group was in the highest baseline quartile. No change in distribution was observed in comparison group. Intervention participants achieved this change by both dietary pattern and vegetable juice consumption. Participants who chose to change dietary pattern without consuming significant quantities of vegetable juice achieved 75% of the level of change observed in other intervention participants.


CONCLUSIONS
Innovative telephone counseling intervention and dietary targets in the Women's Healthy Eating and Living study were associated with the level of change in circulating carotenoid concentration necessary to test the diet and breast cancer hypothesis suggested by cohort studies.
Pierce
John P
JP
Cancer Prevention and Control Program, UCSD Moores Cancer Center, University of California, San Diego, 9500 Gilman Drive, Dept. 0901, La Jolla, CA 92093-0901, USA. jppierce@ucsd.edu
Natarajan
Loki
L
Sun
Shelly
S
Al-Delaimy
Wael
W
Flatt
Shirley W
SW
Kealey
Sheila
S
Rock
Cheryl L
CL
Thomson
Cynthia A
CA
Newman
Vicky A
VA
Ritenbaugh
Cheryl
C
Gold
Ellen B
EB
Caan
Bette J
BJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D001943:Breast Neoplasms / Q000097:blood; D002338:Carotenoids / Q000097:blood* / Q000378:metabolism; D003376:Counseling; D065366:Cryptoxanthins; D015930:Diet Records; D014676:Diet, Vegetarian; D005247:Feeding Behavior; D005260:Female; D005500:Follow-Up Studies; D005518:Food Preferences; D015438:Health Behavior; D006801:Humans; D008019:Life Style; D016014:Linear Models; D014975:Lutein / Q000097:blood; D000077276:Lycopene; D008875:Middle Aged; D010349:Patient Compliance; D017741:Survivors; D024341:Xanthophylls / Q000097:blood; D019207:beta Carotene / Q000097:blood
D015415:Biomarkers; D065366:Cryptoxanthins; D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; C041635:alpha-carotene; D000077276:Lycopene; D014975:Lutein