Genotyping of clinical herpes simplex virus type 1 isolates by use of restriction enzymes.
Recently, three distinct genotypes of clinical herpes simplex virus type 1 (HSV-1) isolates were identified based on DNA sequence information and phylogenetic analysis of clinical isolates and laboratory strains. We utilized single-nucleotide polymorphism within the genes coding for glycoproteins G and I for rapid genotype classification by PCR and restriction enzyme cleavage. The method is suitable for high-scale genotyping of clinical HSV-1 isolates and for the detection of recombinants.
Norberg
Peter
P
Department of Clinical Virology, Göteborg University, Guldhedsgatan 10 B, S-413 46 Göteborg, Sweden. peter.norberg@microbio.gu.se
Bergström
Tomas
T
Liljeqvist
Jan-Ake
JA
D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D005838:Genotype; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000145:classification* / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D014759:Viral Envelope Proteins / Q000235:genetics
D004279:DNA, Viral; D014759:Viral Envelope Proteins; C064959:glycoprotein I, herpes simplex virus type 1; C050591:glycoprotein gG-1, herpes simplex virus type 1; D004262:DNA Restriction Enzymes