title: Actinomycetes (Streptomyces lividans).
abstract: Agrobacterium tumefaciens is best known for its ability to transform plants by delivering the T-DNA that is processed and transferred from the resident Ti plasmid to the recipient plant cells. Less well known is the capacity of this Gram-negative bacterium to transfer its T-DNA into fungi and actinomycetes. Procedures are described on the use of the promiscuous T-DNA transfer system of A. tumefaciens to transform members of the actinomycetes. Integration of the T-DNA derivatives into the Streptomyces chromosome provides opportunities of obtaining valuable mutants of this antibiotic producing soil organism.
authors.affiliation: Davis Crown Gall Group, University of California, Davis, CA, USA.
authors.forename: Clarence I
authors.initials: CI
authors.lastname: Kado
authors.forename: Brian
authors.initials: B
authors.lastname: Kelly
mesh_terms: D039903:Actinobacteria / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D015139:Blotting, Southern; D018929:Cell Culture Techniques; D003470:Culture Media; D004269:DNA, Bacterial; D013171:Spores, Bacterial / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; C036483:T-DNA