Power laws in microrheology experiments on living cells: Comparative analysis and modeling.
We compare and synthesize the results of two microrheological experiments on the cytoskeleton of single cells. In the first one, the creep function J(t) of a cell stretched between two glass plates is measured after applying a constant force step. In the second one, a microbead specifically bound to transmembrane receptors is driven by an oscillating optical trap, and the viscoelastic coefficient Ge(omega) is retrieved. Both J(t) and Ge(omega) exhibit power law behaviors: J(t) = A0(t/t0)alpha and absolute value (Ge(omega)) = G0(omega/omega0)alpha, with the same exponent alpha approximately 0.2. This power law behavior is very robust; alpha is distributed over a narrow range, and shows almost no dependence on the cell type, on the nature of the protein complex which transmits the mechanical stress, nor on the typical length scale of the experiment. On the contrary, the prefactors A0 and G0 appear very sensitive to these parameters. Whereas the exponents alpha are normally distributed over the cell population, the prefactors A0 and G0 follow a log-normal repartition. These results are compared with other data published in the literature. We propose a global interpretation, based on a semiphenomenological model, which involves a broad distribution of relaxation times in the system. The model predicts the power law behavior and the statistical repartition of the mechanical parameters, as experimentally observed for the cells. Moreover, it leads to an estimate of the largest response time in the cytoskeletal network: tau(m) approximately 1000 s.
Balland
Martial
M
Laboratoire Matière et Systèmes Complexes, UMR 7057 associée au CNRS et à l'Université Paris7-Denis Diderot, Case courrier 7056, 2, place Jussieu, 75251 Paris, Cedex 05, France.
Desprat
Nicolas
N
Icard
Delphine
D
Féréol
Sophie
S
Asnacios
Atef
A
Browaeys
Julien
J
Hénon
Sylvie
S
Gallet
François
F
D000818:Animals; D002468:Cell Physiological Phenomena; D048429:Cell Size; D003198:Computer Simulation; D003599:Cytoskeleton / Q000502:physiology*; D004548:Elasticity; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D051379:Mice; D044085:Microfluidics / Q000379:methods*; D008954:Models, Biological; D013314:Stress, Mechanical