Molecular cloning and characterization of the mouse Na+ sulfate cotransporter gene (Slc13a4): Structure and expression.
Sulfate is an essential ion required for numerous functions in mammalian physiology. Due to its hydrophilic nature, cells require sulfate transporters on their plasma membranes to allow entry of sulfate into cells. In this study, we identified a new mouse Na(+)-sulfate cotransporter (mNaS2), characterized its tissue distribution and determined its cDNA and gene (Slc13a4) structures. mNaS2 mRNA was expressed in placenta, brain, lung, eye, heart, testis, thymus and liver. The mouse NaS2 cDNA spans 3384 nucleotides and its open frame encodes a protein of 624 amino acids. Slc13a4 maps to mouse chromosome 6B1 and contains 16 exons, spanning over 40 kb in length. Its 5'-flanking region contains CAAT- and GC-box motifs and a number of putative transcription factor binding sites, including GATA-1, MTF-1, STAT6 and HNF4 consensus sequences. This is the first study to define the tissue distribution of mNaS2 and resolve its cDNA and gene structures, which will allow us to investigate mNaS2 gene expression in vivo and determine its role in mammalian physiology.
Dawson
Paul A
PA
School of Biomedical Sciences, University of Queensland, Brisbane, Australia.
Pirlo
Katrina J
KJ
Steane
Sarah E
SE
Kunzelmann
Karl
K
Chien
Yu Ju
YJ
Markovich
Daniel
D
D024506:5' Flanking Region; D000595:Amino Acid Sequence; D000818:Animals; D027321:Anion Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D050436:Regulatory Elements, Transcriptional; D017386:Sequence Homology, Amino Acid; D000076988:Sulfate Transporters; D027981:Symporters / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
D027321:Anion Transport Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; C400428:SLC13A4 protein, human; C576994:Slc13a4 protein, mouse; D000076988:Sulfate Transporters; D027981:Symporters