[Expression of neuropilin-1 in human laryngeal carcinoma and cell lines].
OBJECTIVE
To study the expression of Neuropilin-1 in human carcinoma and papilloma of larynx specimens and carcinoma cell lines Hep-2.


METHODS
The expression of Neuropilin-1 in human laryngeal carcinoma, papilloma specimens were investigated by immunohistochemical staining; the expression of Neuropilin-1 mRNA in human laryngeal squamous cell carcinoma, papilloma specimens and cell lines were examined by using RT-PCR.


RESULTS
Immunohistochemical analysis revealed that NRP-1 was expressed in all human laryngeal squamous cell carcinoma specimens but was absent in nonmalignant laryngeal tissue. The mRNA of NRP land Hep-2 cell lines are positive in 24 of 28 human laryngeal carcinoma specimens but not negative in nonmalignant laryngeal specimens.


CONCLUSIONS
Neuropilin-1 was expressed in most of human laryngeal carcinoma specimens and cell lines but not expressed in nonmalignant tissue. It suggests that the expression of Neuropilin-1 may be related to the risk and development of laryngeal carcinoma and has high distinguishability.
Zhang
Sulin
S
Department of Otolaryngology Study, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China.
Kong
Weijia
W
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D039942:Neuropilin-1 / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D039942:Neuropilin-1