Crystallization and preliminary X-ray analysis of Atg3.
Atg3 is an E2-like enzyme that catalyzes the conjugation reaction between Atg8 and phosphatidylethanolamine (PE). The Atg8-PE conjugate is essential for autophagy, the bulk degradation process of cytoplasmic components by the vacuolar/lysosomal system. Crystals of Saccharomyces cerevisiae Atg3 have been obtained by the sitting-drop vapour-diffusion method using ammonium sulfate and lithium sulfate as precipitants. A native data set was collected from a single crystal to 2.5 A resolution. The crystals belong to space group P4(1) or P4(3), with unit-cell parameters a = 59.33, c = 115.22 A, and are expected to contain one protein molecule per asymmetric unit.
Yamada
Yuya
Y
Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, N-12, W-6, Kita-ku, Sapporo 060-0812, Japan.
Suzuki
Nobuo N
NN
Fujioka
Yuko
Y
Ichimura
Yoshinobu
Y
Ohsumi
Yoshinori
Y
Inagaki
Fuyuhiko
F
D000645:Ammonium Sulfate / Q000737:chemistry; D000071183:Autophagy-Related Proteins; D003460:Crystallization; D018360:Crystallography, X-Ray; D018020:Lithium Compounds / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D013431:Sulfates / Q000737:chemistry; D044763:Ubiquitin-Conjugating Enzymes
D000071183:Autophagy-Related Proteins; D018020:Lithium Compounds; D029701:Saccharomyces cerevisiae Proteins; D013431:Sulfates; C054097:lithium sulfate; D044763:Ubiquitin-Conjugating Enzymes; C104235:ATG3 protein, S cerevisiae; D000645:Ammonium Sulfate