Long L1 insertions in human gene introns specifically reduce the content of corresponding primary transcripts.
LINE-1 (L1) retrotransposons comprise about 17% of the human genome and include a recently transposed set of Ta-L1 elements that are polymorphic in humans. Although it is widely believed that L1s play an essential role in shaping and functioning of mammalian genomes, the understanding of the impact of L1 insertions on gene expression is far from being comprehensive. Here we compared hnRNA contents for allele pairs of genes heterozygous for Ta-L1 insertions in their introns in human cell lines of various origin. We demonstrated that some Ta-L1 insertions correlated with decreased content of the corresponding hnRNAs. This effect was characteristic of only nearly full-sized L1s and seemed to be tissue specific.
Ustyugova
Svetlana V
SV
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia.
Lebedev
Yuri B
YB
Sverdlov
Eugene D
ED
D000483:Alleles; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D019143:Evolution, Molecular; D015894:Genome, Human; D006367:HeLa Cells; D006579:Heterozygote; D006801:Humans; D007438:Introns; D019169:Jurkat Cells; D020084:Long Interspersed Nucleotide Elements; D012332:RNA, Heterogeneous Nuclear / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D017931:DNA Primers; D012332:RNA, Heterogeneous Nuclear