title: Transcriptional and metabolic profiles of stress-induced, embryogenic tobacco microspores.
abstract: Higher plant microspores, when subjected to various stress treatments in vitro, are able to reprogram their regular gametophytic development towards the sporophytic pathway to form haploid embryos and plants. Suppression subtractive hybridization (SSH) and metabolic profiling were used to characterize this developmental switch. Following differential reverse Northern hybridizations 90 distinct up-regulated sequences were identified in stressed, embryogenic microspores (accessible at www.univie.ac.at/ntsm). Sequence analyses allowed the classification of these genes into functional clusters such as metabolism, chromosome remodeling, signaling, transcription and translation, while the putative functions of half of the sequences remained unknown. A comparison of metabolic profiles of non-stressed and stressed microspores using gas chromatography/mass spectrometry (GC/MS) identified 70 compounds, partly displaying significant changes in metabolite levels, e.g., highly elevated levels of isocitrate and isomaltose in stressed microspores compared to non-stressed microspores. The formation of embryogenic microspores is discussed on the basis of the identified transcriptional and metabolic profiles.
authors.affiliation: Max F. Perutz Laboratories, University Departments at the Campus Vienna Biocenter, Department of Plant Molecular Biology, Vienna University, A-1030, Vienna, Austria, Alisher.Touraev@univie.ac.at.
authors.forename: Julia
authors.initials: J
authors.lastname: Hosp
authors.forename: Alisher
authors.initials: A
authors.lastname: Tashpulatov
authors.forename: Ute
authors.initials: U
authors.lastname: Roessner
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Barsova
authors.forename: Heidrun
authors.initials: H
authors.lastname: Katholnigg
authors.forename: Ralf
authors.initials: R
authors.lastname: Steinborn
authors.forename: Balázs
authors.initials: B
authors.lastname: Melikant
authors.forename: Sergey
authors.initials: S
authors.lastname: Lukyanov
authors.forename: Erwin
authors.initials: E
authors.lastname: Heberle-Bors
authors.forename: Alisher
authors.initials: A
authors.lastname: Touraev
mesh_terms: D015152:Blotting, Northern; D008401:Gas Chromatography-Mass Spectrometry; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013170:Spores / Q000235:genetics* / Q000378:metabolism*; D014026:Nicotiana / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*