title: Identification of the FtsK sequence-recognition domain.
abstract: FtsK is a prokaryotic multidomain DNA translocase that coordinates chromosome segregation and cell division. FtsK is membrane anchored at the division septum and, guided by highly skewed DNA sequences, translocates the chromosome to bring the terminus of replication to the septum. Here, we use in vitro single-molecule and ensemble methods to unveil a mechanism of action in which the translocation and sequence-recognition activities are performed by different domains in FtsK.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA.
authors.forename: Jerod L
authors.initials: JL
authors.lastname: Ptacin
authors.forename: Marcelo
authors.initials: M
authors.lastname: Nöllmann
authors.forename: Carlos
authors.initials: C
authors.lastname: Bustamante
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Cozzarelli
mesh_terms: D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C096452:FtsK protein, E coli; D008565:Membrane Proteins