Phosphodiesterase 7A1 is expressed in human CD4+ naïve T cells at higher levels than in CD4+ memory cells and is not required during their CD3/CD28-dependent activation.
PDE7A1 is a cAMP-hydrolyzing phosphodiesterase expressed in lymphoid tissue, where its possible role during T cell activation remains unclear. We have characterized the functional relevance of PDE7A1 in the naïve (CD4+CD45RA+) and memory (CD4+CD45RO+) subsets of human peripheral CD4+ T cells during CD3/CD28-dependent stimulation. Our results indicate that PDE7A1 is expressed in resting naïve CD4+ T cells at higher levels than in the corresponding memory cells and that levels of PDE7A1 mRNA are not upregulated upon CD3/CD28 mediated stimulation of these T cell subsets. Treatment with a selective inhibitor of PDE7A1 does not impair CD3/CD28 induced activation of naïve or memory CD4+ T cells, nor does it increase intracellular cAMP in CD4+ T cells. We conclude that PDE7A1 is not required during CD3/CD28-dependent activation of naïve and memory CD4+ T cells, but cannot rule out other regulatory roles of PDE7A1 during maturation of CD4+ T cells.
Nueda
Arsenio
A
Departmento de Biología, Centro Investigación Sant Feliu Ll., Almirall, S.A., Ctra. Laureà Miró, 408-410, 08980 Sant Feliu de Llobregat, Barcelona, Spain. anueda@almirall.es
García-Roger
Nuria
N
Domenech
Teresa
T
Godessart
Nuria
N
Cárdenas
Alvaro
A
Santamaría-Babi
Luis F
LF
Beleta
Jorge
J
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000096:biosynthesis*; D015152:Blotting, Northern; D018106:CD28 Antigens / Q000276:immunology / Q000378:metabolism*; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D049109:Cell Proliferation; D054708:Cyclic Nucleotide Phosphodiesterases, Type 7; D005434:Flow Cytometry; D006801:Humans; D007156:Immunologic Memory; D008213:Lymphocyte Activation / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000201:enzymology* / Q000276:immunology
D018106:CD28 Antigens; D017252:CD3 Complex; D012333:RNA, Messenger; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054708:Cyclic Nucleotide Phosphodiesterases, Type 7