Synthesis of a molecularly imprinted polymer for the selective solid-phase extraction of chloramphenicol from honey.
Solid-phase extraction (SPE) with a molecularly imprinted polymer (MIP) as sorbent has been investigated for the clean-up of the broad-spectrum bacteriostatic antibiotic chloramphenicol (CAP) in honey samples. The MIP was prepared by using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EDMA) as cross-linker, chloroform as porogen and CAP as template molecule. The binding behaviour of the template CAP on the MIP was evaluated by high-performance liquid chromatography, and then the MIP was applied as a sorbent in SPE to selectively extract CAP from honey. It was shown that recoveries of nearly 100% of a CAP standard solution and up to 94% from spiked honey samples could be obtained after SPE.
Schirmer
Christina
C
Institute of Dairy Chemistry and Technology, Federal Research Centre for Nutrition and Food (BfEL) Location Kiel, Hermann-Weigmann-Strasse 1, D-24103 Kiel, Germany.
Meisel
Hans
H
D002701:Chloramphenicol / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D006722:Honey / Q000032:analysis*; D008689:Methacrylates / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry
D008689:Methacrylates; D011108:Polymers; D012997:Solvents; C008384:methacrylic acid; D002701:Chloramphenicol; C004919:ethylene dimethacrylate