title: RNA polymerase II gene (RPB2) encoding the second largest protein subunit in Phaeosphaeria nodorum and P. avenaria.
abstract: A 5586 bp sequence (accession no. DQ278491), which includes the RNA polymerase II gene (RPB2) encoding the second largest protein subunit (RPB2), was obtained from the wheat biotype Phaeosphaeria nodorum (PN-w) by PCR amplification. The 3841 bp full length RPB2 gene contains two exons and a 52 bp intron, and encodes a complete 1262 amino acid protein. Similar to the C-terminals of the beta subunits of prokaryotes and yeast RNA polymerases, the deduced RPB2 protein contained many structural features needed for gene transcription. Based on the phylogenetic analysis with the deduced RPB2 polypeptide sequences, the PN-w was closely related to the maize pathogen Cochliobolus heterostrophus. Size differences were found in the full length RPB2 gene of cereal Phaeosphaeria species, mainly due to differences in intron size. No nucleotide substitutions were found in homothallic P. avenaria f.sp. triticea (Pat1) and barley biotype P. nodorum (PN-b) isolates used in this study. The nucleotide and deduced amino acid sequences of the RPB2 gene in Pat1 were closely related to that in PN-w.
authors.affiliation: Department of Plant Pathology, Plant Breeding and Acclimatization Institute, Radzikow, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Malkus
authors.forename: Pi-Fang Linda
authors.initials: PF
authors.lastname: Chang
authors.forename: Sabina M
authors.initials: SM
authors.lastname: Zuzga
authors.forename: Kuang-Ren
authors.initials: KR
authors.lastname: Chung
authors.forename: Jonathan
authors.initials: J
authors.lastname: Shao
authors.forename: Barry M
authors.initials: BM
authors.lastname: Cunfer
authors.forename: Edward
authors.initials: E
authors.lastname: Arseniuk
authors.forename: Peter P
authors.initials: PP
authors.lastname: Ueng
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D005656:Fungal Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D021122:Protein Subunits / Q000235:genetics; D012319:RNA Polymerase II / Q000235:genetics*; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014908:Triticum / Q000382:microbiology
chemical_list: D005656:Fungal Proteins; D021122:Protein Subunits; D012331:RNA, Fungal; D012319:RNA Polymerase II