title: [Prevalence of celiac disease serological markers in Tunisian type 1 diabetic adults].
abstract: OBJECTIVE
  to evaluate the prevalence of celiac serological markers; anti-transglutaminase (ATGt), anti-endomysium (AE), anti-gliadin (AGD) and anti-reticulin (AR) antibodies; in type 1 diabetic Tunisian adults.
  
  
  METHODS
  261 type 1 diabetic patients aged from 16 to 60 years were enrolled in this prospective study. IgG and IgA transglutaminase and gliadin were measured with ELISA. IgA AE were tested by indirect immunofluorescence using 2 substrates; monkey oesophagus and human umbilical cord. AR were detected by indirect immunofluorescence on rat liver, kidney and stomach. Sera IgA level was measured by turbidimetry.
  
  
  RESULTS
  83/261 of diabetics were positive for at least one antibody, 5.7% had ATGt-A, 3.4% AE on monkey esophagus, 3.1% AE on umbilical cordon, 18% AGD-A, 19.5% AGD-G and 3.1% AR. There was an excellent concordance between AE and ATGt (r = 0.9). Out of the 261 diabetics, 5 had an IgA deficiency and one of them has IgG AE and ATGt.
  
  
  CONCLUSIONS
  serological markers of celiac disease seem to be frequent in diabetics. Nevertheless, diagnosis must be confirmed by histological studies which allow us to know the real prevalence of celiac disease in diabetic adults.
authors.affiliation: Laboratoire d'Immunologie, Hôpital La Rabta, Tunis.
authors.forename: L
authors.initials: L
authors.lastname: Laadhar
authors.forename: M
authors.initials: M
authors.lastname: Zitouni
authors.forename: M
authors.initials: M
authors.lastname: Kallel-Sellami
authors.forename: S
authors.initials: S
authors.lastname: Masmoudi
authors.forename: R
authors.initials: R
authors.lastname: Bouguerra
authors.forename: H
authors.initials: H
authors.lastname: Chaabouni
authors.forename: C
authors.initials: C
authors.lastname: Ben Slama
authors.forename: S
authors.initials: S
authors.lastname: Makni
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers; D002446:Celiac Disease / Q000175:diagnosis / Q000453:epidemiology* / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000150:complications*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005903:Gliadin / Q000276:immunology; D000882:Haplorhini; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D008297:Male; D008875:Middle Aged; D018485:Muscle Fibers, Skeletal / Q000276:immunology; D009391:Nephelometry and Turbidimetry; D015995:Prevalence; D011446:Prospective Studies; D051381:Rats; D012155:Reticulin / Q000276:immunology; D011503:Transglutaminases / Q000276:immunology; D014416:Tunisia
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D007070:Immunoglobulin A; D012155:Reticulin; D005903:Gliadin; D011503:Transglutaminases