Tetrabutylammonium-induced coacervation in vesicular solutions of alkyl carboxylic acids for the extraction of organic compounds.
The potential of the tetrabutylammonium-induced liquid-liquid-phase separation in alkyl carboxylic acid vesicular solutions for the extraction of organic compounds prior to liquid chromatography was examined for the first time. The behavior of the coacervates yielded from octanoic to oleic acids as a function of the pH and salts was investigated. The time required for phase separation depended on the length of the carboxylic acid alkyl chain and the experimental procedure (i.e., standing, sonication, centrifugation, stirring, etc.). Theoretical preconcentration factors were a function of both surfactant concentration and the length of the alkyl chain, and they greatly surpassed those obtained with other surfactant-mediated separations (e.g., surfactant-rich phases from dodecanesulfonic acid or Triton X-114). Parameters affecting the extraction efficiency were assessed. Analytes in a wide polarity/charge range, (e.g., PAHs, surfactants, chlorophenols, bisphenols, phthalates, herbicides, amines, dyes, and photographic developers) were extracted with high efficiencies on the basis of the different types of interactions that the vesicular coacervates can establish (i.e., hydrophobic and ionic interactions, hydrogen bonds, and formation of mixed aggregates). The coacervates were compatible with the chromatographic determination of analytes following UV or MS detection. Their suitability for working under real conditions was checked by applying them to the extraction of nonionic surfactants [alkylphenol ethoxylates (octyl and nonyl) and alcohol ethoxylates (C12-C16)] from raw and treated sewage and to river water samples. Analytes in the coacervate were separated and quantified by liquid chromatography-ion trap mass spectrometry. No cleanup steps were necessary. Recoveries of the target compounds in the environmental water samples ranged from 89 to 103%.
Ruiz
Francisco-Javier
FJ
Department of Analytical Chemistry, Facultad de Ciencias, Edificio Anexo Marie Curie, Campus de Rabanales, 14071-Córdoba, Spain.
Rubio
Soledad
S
Pérez-Bendito
Dolores
D