Potato (Solanum tuberosum L.).
Potato (Solanum tuberosum L.) is a globally important crop plant producing high yields of nutritionally valuable food in the form of tubers. It has been the focus of substantial study because of its use both as a staple food crop and as a potentially significant source of compounds of interest. This has included the development and application of transgenic technology for introducing novel traits of fundamental and applied interest. This chapter describes a rapid, efficient, and cost-effective system for the routine transformation of this crop plant at rates above 40% efficiency, calculated as the mean number of Southern blot- confirmed independent transgenics per number of internodal explants originally plated. Internodal sections are co-cultivated with Agrobacterium tumefaciens and subjected to a two-stage callus induction/shoot outgrowth system under kanamycin selection. Shoot regeneration rates are high using the described method, and excised independent shoots rooting from the cut end of the stem after two further subcultures on kanamycin are 95% certain to be transformed. The transgenic status can be confirmed by molecular analysis and the plants grown on for tuber production enabling a wide spectrum of further studies.
Millam
Steve
S
Institute of Molecular Plant Science, University of Edinburgh, EH9 3JR, The United Kingdom.
D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D003470:Culture Media; D005822:Genetic Vectors; D030821:Plants, Genetically Modified / Q000033:anatomy & histology / Q000254:growth & development*; D011198:Solanum tuberosum / Q000033:anatomy & histology / Q000235:genetics*; D014170:Transformation, Genetic
D003470:Culture Media