[Expression of Ezrin in gastric carcinoma and its significance].
OBJECTIVE
To investigate the expression of Ezrin in gastric cancer, its role in tumor metastasis.


METHODS
Ezrin expression in tumor tissues from 90 gastric cancer cases and in normal gastric mucosa from 12 cases with benign disease was examined by immunohistochemical staining. Ezrin expression in gastric cancer cell lines was also detected by Western blot, and in vitro invasion assay was used to examine the invasive ability of the cell lines.


RESULTS
The expression rate of Ezrin was significantly higher in gastric cancer tissues than that in normal tissues (P< 0.05), and significantly correlated with lymph node metastasis (P< 0.05). Western blot showed that MKN-45 cell line had the highest expression of Ezrin among 5 gastric cancer cells. MKN-45 possessed highest invasion ability.


CONCLUSIONS
Ezrin expression is up-regulated, and may be associated with lymph node metastasis in gastric cancer.
Shi
Rong-liang
RL
Department of Surgery, Ruijin Hospital, School of Medical, Shanghai Jiaotong University, Shanghai Institute of Digestive Surgery, Shanghai 200025, China.
Li
Jian-fang
JF
Qu
Ying
Y
Chen
Xue-hua
XH
Gu
Qin-long
QL
Zhu
Zheng-gang
ZG
Liu
Bing-ya
BY
D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D040081:Protein Array Analysis; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*; D000099149:Ezrin
D003598:Cytoskeletal Proteins; D000099149:Ezrin