title: [Investigation on bovine leukocyte adhesion deficiency].
abstract: Bovine leukocyte adhesion deficiency (BLAD) is autosomal recessive disease. The pathogeny of BLAD is genic mutation of CD18-integrins on the leukocyte. In order to know the carrier and occurrence of bovine leukocyte adhesion deficiency (BLAD) among cows age from one to six years old in China, 1,000 cows were investigated by means of amplifying a CD18 gene fragment via reverse transcriptase-PCR followed by restriction digestion with Taq I. Results showed that 19 cows were BLAD carriers, indicating that the BLAD carrier rate was 1.9 percent. In addition, one cow was found to have BLAD.
authors.affiliation: College of Life Science and Biotechnology, Heilongjiang August First Land Reclamation University, Daqing 163319, China. majinzhu1976@163.com
authors.forename: Jin-Zhu
authors.initials: JZ
authors.lastname: Ma
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Cui
authors.forename: Zhan-Bo
authors.initials: ZB
authors.lastname: Zhu
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Cao
authors.forename: Fan-Ze
authors.initials: FZ
authors.lastname: Piao
mesh_terms: D000818:Animals; D018821:CD18 Antigens / Q000235:genetics / Q000378:metabolism; D002417:Cattle / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000235:genetics* / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018821:CD18 Antigens; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases