Force measurements between emulsion droplets-ssDNA conjugates: a new tool for medical diagnostics.
We describe here a new system involving direct force measurements between biomolecules that could be used in biomedical diagnostics. The method consists in the use of magnetic emulsion droplets bearing immobilized single stranded DNA fragments (ssDNA, Deoxyribo Nucleic Acid). The immobilized ssDNA fragments are able to recognize complementary DNA molecules via specific hydrogen binding (hybridization process). The ssDNA used in this study are 32 bases oligonucleotides functionalized at their 5' extremity with biotin and then immobilized onto the magnetic nanodroplets via interactions with streptavidin previously chemically grafted onto the nanomagnetic support. The aim of this work is to evaluate the possible detection of captured nucleic acid targets via single force measurements as an alternative to classical ELOSA (Enzyme Linked Oligo Sorbent Assay). The obtained results are discussed mainly in terms of electrostatic interactions.
Montagne
F
F
CNRS - bioMérieux, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon 07, France.
Braconnot
S
S
Eläissari
A
A
Pichot
C
C
Daniel
J C
JC
Mandrand
B
B
Mondain-Monval
O
O
D000327:Adsorption; D001703:Biophysics / Q000379:methods; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D053938:DNA Fragmentation; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004655:Emulsions; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry; D009821:Oils; D019809:Streptavidin / Q000737:chemistry*; D014867:Water
D004277:DNA, Single-Stranded; D004655:Emulsions; D009696:Nucleic Acids; D009821:Oils; D014867:Water; D001710:Biotin; D019809:Streptavidin