From array to array: confirmation of genomic gains and losses discovered by array-based comparative genomic hybridization utilizing fluorescence in situ hybridization on tissue microarrays.
The combination of array-based comparative genomic hybridization (CGH) with fluorescence in situ hybridization utilizing custom-designed bacterial artificial chromosome (BAC) probes applied to tissue microarrays represents a powerful compendium of techniques-greatly enhancing the throughput of genomic analysis and subsequent target validation. Such approach can be automated at various levels and allows managing large volume of targets and samples in a few experiments. As such, this approach facilitates discovery, validation and implementation of findings in the process of identification of new diagnostic, prognostic and potentially therapeutic molecular markers.
Skacel
Marek
M
Department of Anatomic Pathology, The Cleveland Clinic Foundation, L-25, Cleveland, OH 44191, USA. skacelm@ccf.org
Siva
Ana
A
Xu
Bo
B
Tubbs
Raymond R
RR
D004247:DNA / Q000235:genetics; D023281:Genomics / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D046888:Tissue Array Analysis / Q000379:methods*
D004247:DNA