Pediococcus ethanolidurans sp. nov., isolated from the walls of a distilled-spirit-fermenting cellar.
Two novel Pediococcus strains, Z-9(T) and Z-17, were isolated from a distilled-spirit-fermenting cellar in Hebei Province, China. The cells were Gram-positive, non-spore-forming, non-motile cocci and occurred in pairs or tetrads. The strains were facultatively anaerobic and produced only lactic acid [d(-) and l(+) isomers in the ratio 50 : 50] from glucose fermentation. Catalase activity was not present. Both strains were able to grow in 6.5 % ethanol and at pH 3.5, but not in 4 % NaCl. The mean genomic G+C content of the two strains was 39.5+/-0.5 mol% (39 mol% for the type strain, Z-9(T)). The levels of 16S rRNA gene sequence similarity between the two novel strains and related species of the genus Pediococcus ranged from 98.3 to 98.7 %. The levels of DNA-DNA relatedness between strain Z-9(T) and the phylogenetically closely related pediococci Pediococcus damnosus LMG 11484(T), Pediococcus inopinatus LMG 11409(T), Pediococcus parvulus LMG 11486(T) and Pediococcus cellicola LMG 22956(T) were 14.6, 33, 28.7 and 16.8 %, respectively. On the basis of phenotypic, genotypic and phylogenetic analyses, a novel species, Pediococcus ethanolidurans sp. nov., is proposed, with strain Z-9(T) (=AS 1.3889(T)=LMG 23354(T)) as the type strain.
Liu
Limeng
L
College of Life Science, Hebei University, Baoding, Hebei 071002, P. R. China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P. R. China.
Zhang
Bin
B
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P. R. China.
Tong
Huichun
H
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P. R. China.
Dong
Xiuzhu
X
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P. R. China.
D000434:Alcoholic Beverages / Q000382:microbiology*; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D020459:Genes, rRNA; D005838:Genotype; D019344:Lactic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010376:Pediococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; D000431:Ethanol