G-protein coupled receptor kinase-like immunoreactivity in the snail, Helix pomatia, neurons.
GPRCs are regulated via phosphorylation by different protein kinases including GRKs and PKA and PKC. The purpose of this study was to determine the presence and physiological role of GRKs in the tissues of the snail, Helix pomatia. Here we report that immunoblotting of brain homogenate with anti-GRK2/3 antibody prepared from mammalian tissue can be detected in snail GRK-like immunoreactivity. The GRK2/3 immunoreactivity was found at approximately 80 kDa in a variety of cells, including salivary duct, salivary gland and eye. Intracellular injection of the anti-GRK2/3 prevented the neuron from desensitization and agonist-induced activation augmented the phosphorylated GRKs in the membrane fraction suggesting that GRKs may have a functional role in the neuropeptide receptor desensitization in snail.
Pirger
Zsolt
Z
Department of Experimental Zoology, Balaton Limnological Research Institute, Hungarian Academy of Sciences, Tihany, Hungary.
László
Zita
Z
Kiss
Tibor
T
D000818:Animals; D005123:Eye / Q000378:metabolism; D006372:Helix, Snails / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D018010:Receptors, Invertebrate Peptide / Q000378:metabolism; D018987:Salivary Ducts / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D051552:beta-Adrenergic Receptor Kinases / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D043562:Receptors, G-Protein-Coupled; D018010:Receptors, Invertebrate Peptide; D017346:Protein Serine-Threonine Kinases; D051552:beta-Adrenergic Receptor Kinases