[Effects of acupuncture on expressions of transcription factors NF-E2, YB-1, LRG47 in the SAMP10 mouse].
OBJECTIVE
To explore the mechanism of acupuncture in delaying aging.


METHODS
Using SAMP10 mice and normal control SAMR1 as model and applying RT-PCR and DIG probed Northern blot techniques to observe expression of NF-E2, YB-1, LRG47 genes in whole brain, cortex and hippocampus in the 8-month SAMR1 control group, 8-month SAMP10 control group, 8-month SAMP10 acupuncture group and 8-month SAMP10 non-point acupuncture group.


RESULTS
In the SAMP10 control group, the expression of NF-E2, YB-1 and LRG47 were down-regulated in the whole brain, cortex and hippocampus, and after acupuncture they were up-regulated and tended to normal.


CONCLUSIONS
Aging of the SAMP10 mouse brain is related with expression of NF-E2, YB-1 and LRG47 genes, and acupuncture can regulate the expression of NF-E2, YB-1 and LRG47 genes, improving the functions of erythrocyte series, increasing proliferation of cells and immune function of cells in anti-bacteria, hence anti-aging.
Fu
Yu
Y
First Teaching Hospital of Tianjin TCM College, Tianjin 300193, China.
Yu
Jian-chun
JC
Ding
Xiao-rong
XR
Han
Jing-xian
JX
D015670:Acupuncture Therapy; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D050984:NF-E2 Transcription Factor, p45 Subunit / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051840:Y-Box-Binding Protein 1 / Q000235:genetics*
C096000:Ifi1 protein, mouse; D050984:NF-E2 Transcription Factor, p45 Subunit; C494693:Nfe2 protein, mouse; D012333:RNA, Messenger; D051840:Y-Box-Binding Protein 1; D019204:GTP-Binding Proteins