title: Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L.
abstract: The defatted seed meal of common vetch (Vicia sativa L.) served as a source of (R)-oxynitrilase which catalyzed the enantioselective addition of HCN to aromatic, heteroaromatic, fluoro-substituted aromatic aldehydes to produce the corresponding enantiomeric pure cyanohydrins in yields of 52-100% and 88-99% ee at 12 degrees C under micro-aqueous medium (diisopropyl ether) without addition of buffer solution.
authors.affiliation: College of Pharmacy and Life Science, Nanjing University of Technology, Nanjing, China. hanshiqing@njut.edu.cn
authors.forename: Shi-Qing
authors.initials: SQ
authors.lastname: Han
authors.forename: Ping-Kai
authors.initials: PK
authors.lastname: Ouyang
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000446:Aldehyde-Lyases / Q000138:chemical synthesis / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods; D001711:Biotransformation; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009570:Nitriles / Q000138:chemical synthesis / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D010944:Plants / Q000378:metabolism; D013237:Stereoisomerism; D013696:Temperature; D033022:Vicia sativa / Q000201:enzymology*
chemical_list: D009570:Nitriles; D010936:Plant Extracts; C430593:cyanohydrin; D000446:Aldehyde-Lyases; C021541:mandelonitrile lyase