Soluble guanylate cyclase.
Soluble guanylate cyclase (sGC) is a mammalian nitric oxide (NO) sensor. When NO binds to the sGC heme, its GTP cyclase activity markedly increases, thus generating cyclic GMP, which serves to regulate several cell signaling functions. A good deal is known about the kinetics and equilibrium of binding of NO to sGC, leading to a proposed multistep mechanism of sGC activation that involves at least two NO-binding sites. The crystal structure of a member of a recently discovered family of prokaryotic sGC homologues has provided important insights into structure-function relationships within the sGC family of proteins.
Poulos
Thomas L
TL
Department of Molecular Biology & Biochemistry, University of California Irvine, Irvine, CA 92697-3900, USA. poulos@uci.edu
D000818:Animals; D004789:Enzyme Activation; D006162:Guanylate Cyclase / Q000737:chemistry* / Q000378:metabolism*; D006418:Heme / Q000737:chemistry; D066298:In Vitro Techniques; D007700:Kinetics; D008024:Ligands; D008958:Models, Molecular; D009569:Nitric Oxide / Q000378:metabolism; D011387:Prokaryotic Cells; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction; D012995:Solubility
D008024:Ligands; D011994:Recombinant Proteins; D009569:Nitric Oxide; D006418:Heme; D006162:Guanylate Cyclase