Fluorescence lifetime imaging microscope consisting of a compact picosecond dye laser and a gated charge-coupled device camera for applications to living cells.
An inverted microscope was combined with a compact dye laser with a pulse width of <190 ps and an intensified charge-coupled device (ICCD) camera with a minimum gate width of 200 ps. The resulting fluorescence lifetime imaging microscope, which has a temporal resolution of 340 ps, was used to measure the fluorescence lifetime of polymer microspherers. The results indicated a fluorescence lifetime of 0.9 ns. The present analytical instrument was also employed in an evaluation of biological cells after labeling them with SYTO 13, a fluorescent dye.
Uchimura
Tomohiro
T
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
Kawanabe
Satoshi
S
Maeda
Yuki
Y
Imasaka
Totaro
T
D045744:Cell Line, Tumor; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D007834:Lasers; D008027:Light; D008175:Lung Neoplasms / Q000473:pathology; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D018715:Microscopy, Video; D008863:Microspheres; D055095:Optics and Photonics; D009991:Oscillometry; D011137:Polystyrenes / Q000737:chemistry; D013997:Time Factors
D005456:Fluorescent Dyes; D011137:Polystyrenes