Kribbia dieselivorans gen. nov., sp. nov., a novel member of the family Intrasporangiaceae.
Two Gram-positive, catalase-positive, irregular short rod- or coccoid-shaped bacterial strains, N113(T) and R33, were isolated from an enrichment culture with diesel oil-degradation activity and their taxonomic positions were investigated using a polyphasic approach. Phenotypic, phylogenetic and genetic similarities indicated that strains N113(T) and R33 were representatives of the same species. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains N113(T) and R33 form a lineage independent from those of members of the family Intrasporangiaceae. The novel isolates had cell-wall peptidoglycan based on meso-diaminopimelic acid, MK-8(H(4)) as the predominant menaquinone and 10-methyl-C(18 : 0), iso-C(16 : 0), C(18 : 1)omega9c, C(16 : 0) and C(18 : 0) as the major cellular fatty acids. The DNA G+C contents were 69.6-69.9 mol%. These chemotaxonomic properties, together with phylogenetic distinctiveness, distinguish the two novel strains from recognized members of the family Intrasporangiaceae. On the basis of phenotypic, phylogenetic and genetic data, strains N113(T) (=KCTC 19143(T)=JCM 13585(T)) and R33 are classified as representatives of a novel genus and species, Kribbia dieselivorans gen. nov., sp. nov., within the family Intrasporangiaceae.
Jung
Seo-Youn
SY
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, South Korea.
Kim
Hee-Sik
HS
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, South Korea.
Song
Jae Jun
JJ
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, South Korea.
Lee
Seung-Goo
SG
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, South Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, South Korea.
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, South Korea.
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005742:Gasoline; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology*; D007723:Korea; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D005227:Fatty Acids; D005742:Gasoline; D012336:RNA, Ribosomal, 16S