title: Stanniocalcin-1 regulates endothelial gene expression and modulates transendothelial migration of leukocytes.
abstract: The mammalian counterpart of the fish calcium-regulating hormone stanniocalcin-1 (STC1) inhibits monocyte chemotactic protein-1- and stromal-derived factor-1alpha (SDF-1alpha)-mediated chemotaxis and diminishes chemokinesis in macrophage-like RAW264.7 and U937 cells in a manner that may involve attenuation of the intracellular calcium signal. STC1 is strongly induced in the kidney following obstructive injury. We hypothesized that STC1 may serve to attenuate the influx of inflammatory cells to the site of tissue injury. In this study, we examined the effect of STC1 on the migration of freshly isolated human macrophages, neutrophils, and T and B lymphocytes through quiescent or IL-1beta-treated human umbilical vein endothelial cell (HUVEC) monolayers. STC1 inhibited transmigration of macrophages and T lymphocytes through quiescent or IL-1beta-activated HUVECs but did not attenuate the transmigration of neutrophils and B lymphocytes. STC1 regulates gene expression in cultured endothelial cells and is detected on the apical surface of endothelial cells in vivo. The data suggest that STC1 plays a critical role in transendothelial migration of inflammatory cells and is involved in the regulation of numerous aspects of endothelial function.
authors.affiliation: Renal Section, Dept. of Medicine, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Arup
authors.initials: A
authors.lastname: Chakraborty
authors.forename: Heddwen
authors.initials: H
authors.lastname: Brooks
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Wayne
authors.initials: W
authors.lastname: Smith
authors.forename: Matthew R
authors.initials: MR
authors.lastname: McReynolds
authors.forename: Jay B
authors.initials: JB
authors.lastname: Hoying
authors.forename: Roger
authors.initials: R
authors.lastname: Bick
authors.forename: Luan
authors.initials: L
authors.lastname: Truong
authors.forename: Brian
authors.initials: B
authors.lastname: Poindexter
authors.forename: Hui
authors.initials: H
authors.lastname: Lan
authors.forename: Wafa
authors.initials: W
authors.lastname: Elbjeirami
authors.forename: David
authors.initials: D
authors.lastname: Sheikh-Hamad
mesh_terms: D002118:Calcium / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D006023:Glycoproteins / Q000502:physiology*; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D007668:Kidney / Q000098:blood supply; D008264:Macrophages / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D013601:T-Lymphocytes / Q000502:physiology
chemical_list: D006023:Glycoproteins; D053583:Interleukin-1beta; C051795:teleocalcin; D002118:Calcium