[Screening of peafowl microsatellite primers and analysis of genetic diversity].
The applicability of chicken microsatellite primers to peafowl population was analyzed in the present paper, and the results showed 14 of 29 pairs of microsatellite primers from chicken could amplify peafowl DNA and produce specific allele patterns. A mean of 1.71 alleles was found for each locus. Seven pairs were highly polymorphic, and MCW0080 and MCW0098 were ideal markers for peafowl. Genetic diversity analysis within and between the green peafowl and the blue peafowl populations demonstrated that the expected heterozygosity of two peafowl populations were 0.2482 and 0.2744, respectively. The inbreeding index (FST), Reynolds' genetic distance and gene flow between the two populations were 0.078, 0.0603 and 3.896 respectively. These results indicate that the heterozygosity and the genetic diversity of these two peafowl populations were very low, and suggest a tendency towards intermixing.
Bao
Wen-Bin
WB
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. wbbao1974@163.com
Chen
Guo-Hong
GH
Shu
Jing-Ting
JT
Xu
Qi
Q
Li
Hui-Fang
HF
D000483:Alleles; D000818:Animals; D017931:DNA Primers / Q000235:genetics*; D046094:Galliformes / Q000235:genetics*; D005787:Gene Frequency; D014644:Genetic Variation; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction
D017931:DNA Primers