title: Crystallization and preliminary crystallographic analysis of p40phox, a regulatory subunit of NADPH oxidase.
abstract: p40(phox) is a cytosolic component of the phagocyte NADPH oxidase, which is responsible for production of the superoxide that kills invasive microorganisms. Full-length p40(phox) was expressed in Escherichia coli, purified and crystallized by the sitting-drop vapour-diffusion method at 293 K using polyethylene glycol 20,000 as a precipitant. Diffraction data were collected to 3.0 A resolution at 100 K using synchrotron radiation. The crystal belongs to space group C222(1), with unit-cell parameters a = 146.27, b = 189.81, c = 79.88 A. This crystal was estimated to contain two or three protein molecules per asymmetric unit from the acceptable range of volume-to-weight ratio values.
authors.affiliation: Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, N-12, W-6, Kita-ku, Sapporo 060-0812, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Honbou
authors.forename: Satoru
authors.initials: S
authors.lastname: Yuzawa
authors.forename: Nobuo N
authors.initials: NN
authors.lastname: Suzuki
authors.forename: Yuko
authors.initials: Y
authors.lastname: Fujioka
authors.forename: Hideki
authors.initials: H
authors.lastname: Sumimoto
authors.forename: Fuyuhiko
authors.initials: F
authors.lastname: Inagaki
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D019255:NADPH Oxidases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D021122:Protein Subunits / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D021122:Protein Subunits; D019255:NADPH Oxidases; C113333:NCF4 protein, human