Low-level laser therapy can reduce lipopolysaccharide-induced contractile force dysfunction and TNF-alpha levels in rat diaphragm muscle.
Our objective was to investigate if low-level laser therapy (LLLT) could improve respiratory function and inhibit tumor necrosis factor (TNF-alpha) release into the diaphragm muscle of rats after an intravenous injection of lipopolysaccharide (LPS) (5 mg/kg). We randomly divided Wistar rats in a control group without LPS injection, and LPS groups receiving either (a) no therapy, (b) four sessions in 24 h with diode Ga-AsI-Al laser of 650 nm and a total dose of 5.2 J/cm2, or (c) an intravenous injection (1.25 mg/kg) of the TNF-alpha inhibitor chlorpromazine (CPZ). LPS injection reduced maximal force by electrical stimulation of diaphragm muscle from 24.15+/-0.87 N in controls, but the addition of LLLT partly inhibited this reduction (LPS only: 15.01+/-1.1 N vs LPS+LLLT: 18.84+/-0.73 N, P<0.05). In addition, this dose of LLLT and CPZ significantly (P<0.05 and P<0.01, respectively) reduced TNF-alpha concentrations in diaphragm muscle when compared to the untreated control group.
Aimbire
F
F
Research Group of Animal Experimental, IP & D UNIVAP R. Shishima Hifumi, 2911, 12240-000, São José dos Campos, SP, Brazil.
Lopes-Martins
R A B
RA
Castro-Faria-Neto
H C
HC
Albertini
R
R
Chavantes
M C
MC
Pacheco
M T T
MT
Leonardo
P S L M
PS
Iversen
V V
VV
Bjordal
J M
JM
D000818:Animals; D002746:Chlorpromazine / Q000494:pharmacology; D003964:Diaphragm / Q000737:chemistry* / Q000187:drug effects; D008070:Lipopolysaccharides; D028022:Low-Level Light Therapy; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012131:Respiratory Insufficiency / Q000139:chemically induced* / Q000628:therapy*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000037:antagonists & inhibitors
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D002746:Chlorpromazine