title: Silencing of natural interferon producing cell activation by porcine circovirus type 2 DNA.
abstract: Porcine circovirus type 2 (PCV2) infection of natural interferon producing cells (NIPCs) impairs the induction of interferon (IFN)-alpha and tumour necrosis factor (TNF)-alpha by cytosine-phosphorothioate-guanine (CpG) oligodeoxynucleotides (ODNs), thereby preventing both their autocrine maturation and the paracrine maturation of myeloid dendritic cells (DCs). The present study shows that the PCV2-mediated inhibition of NIPCs was mediated by viral DNA, although it was independent of virus replication. The inhibitory effect of PCV2 DNA was more diversified than if it had simply targeted CpG-ODN-induced cytokines (IFN-alpha, TNF-alpha, interleukin-6, IL-12). A broad spectrum inhibition was noted, affecting responses induced by toll-like receptor (TLR)-7 and TLR9 agonists, as well as viruses including pseudorabies virus, transmissible gastroenteritis virus and classical swine fever virus. From these results, it would appear that PCV2 DNA can induce a dominant negative signal influencing independent pattern recognition receptor-induced activation cascades. Despite a concomitant internalization of PCV2 DNA and CpG-ODNs, no colocalization was observed, indicating that PCV2 DNA and CPG-ODNs may not target the same receptor. This study describes a novel modulation of the innate immune response, which would render the host more susceptible to secondary or concomitant microbial infections.
authors.affiliation: Institute of Virology and Immunoprophylaxis, Mittelhäusern, Switzerland.
authors.forename: Isabelle E
authors.initials: IE
authors.lastname: Vincent
authors.forename: Carole
authors.initials: C
authors.lastname: Balmelli
authors.forename: Brian
authors.initials: B
authors.lastname: Meehan
authors.forename: Gordon
authors.initials: G
authors.lastname: Allan
authors.forename: Artur
authors.initials: A
authors.lastname: Summerfield
authors.forename: Kenneth C
authors.initials: KC
authors.lastname: McCullough
mesh_terms: D000818:Animals; D002213:Capsid / Q000276:immunology; D017925:Circovirus / Q000235:genetics* / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis; D004279:DNA, Viral / Q000276:immunology*; D020868:Gene Silencing; D007108:Immune Tolerance; D016898:Interferon-alpha / Q000096:biosynthesis*; D009838:Oligodeoxyribonucleotides / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology; D013552:Swine; D051193:Toll-Like Receptors / Q000276:immunology; D014779:Virus Replication
chemical_list: C408982:CPG-oligonucleotide; D016207:Cytokines; D004279:DNA, Viral; D016898:Interferon-alpha; D009838:Oligodeoxyribonucleotides; D051193:Toll-Like Receptors