title: Calcium-sensing receptor stimulation induces nonselective cation channel activation in breast cancer cells.
abstract: The calcium-sensing receptor (CaR) is expressed in epithelial ducts of both normal human breast and breast cancer tissue, as well as in the MCF-7 cell line as assessed by immunohistochemistry and Western blot analysis. However, to date, there are no data regarding the transduction pathways of CaR in breast cancer cells. In this study, we show that a CaR agonist, spermine, and increased extracellular Ca(2+) ([Ca(2+)](o)) sequentially activate two inward currents at -80 mV. The first was highly permeable to Ca(2+) and inhibited by 2-aminophenyl borate (2-APB). In contrast, the second was more sensitive to Na(+) and Li(+) than to Ca(2+) and insensitive to 2-APB. Furthermore, intracellular dialysis with high Mg(2+), flufenamic acid or amiloride perfusion was without any effect on the second current. Both currents were inhibited by La(3+). Calcium imaging recordings showed that both [Ca(2+)](o) and spermine induced an increase in intracellular calcium ([Ca(2+)](i)) and that removal of extracellular Ca(2+) or perfusion of 2-APB caused a decline in [Ca(2+)](i). It is well known that stimulation of CaR by an increase in [Ca(2+)](o) or with spermine is associated with activation of phospholipase C (PLC). Inhibition of PLC reduced the [Ca(2+)](o)-stimulated [Ca(2+)](i) increase. Lastly, reverse-transcriptase polymerase chain reaction showed that MCF-7 cells expressed canonical transient receptor potential (TRPCs) channels. Our results suggest that, in MCF-7 cells, CaR is functionally coupled to Ca(2+)-permeable cationic TRPCs, for which TRPC1 and TRPC6 are the most likely candidates for the highly selective Ca(2+) current. Moreover, the pharmacology of the second Na(+) current excludes the involvement of the more selective Na(+) transient receptor potential melastatin (TRPM4 and TRPM5) and the classical epithelial Na(+ )channels.
authors.affiliation: Laboratoire de Physiologie Cellulaire et Moléculaire, EA 2086, Faculté des Sciences, Université de Picardie Jules Verne, 33 rue Saint-leu, 80039, Amiens, France.
authors.forename: Yassine
authors.initials: Y
authors.lastname: El Hiani
authors.forename: Ahmed
authors.initials: A
authors.lastname: Ahidouch
authors.forename: Morad
authors.initials: M
authors.lastname: Roudbaraki
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Guenin
authors.forename: Gérard
authors.initials: G
authors.lastname: Brûlé
authors.forename: Halima
authors.initials: H
authors.lastname: Ouadid-Ahidouch
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000494:pharmacology; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D045744:Cell Line, Tumor; D005439:Flufenamic Acid / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D020033:Protein Isoforms / Q000235:genetics / Q000502:physiology; D044169:Receptors, Calcium-Sensing / Q000819:agonists / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013096:Spermine / Q000494:pharmacology; D050052:TRPC Cation Channels / Q000235:genetics / Q000502:physiology*; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D001896:Boron Compounds; D020033:Protein Isoforms; D044169:Receptors, Calcium-Sensing; D050052:TRPC Cation Channels; D013096:Spermine; D005439:Flufenamic Acid; C109986:2-aminoethoxydiphenyl borate; D010738:Type C Phospholipases; D008274:Magnesium; D002118:Calcium