Effects of oleic acid on the high threshold barium current in seabass Dicentrarchus labrax ventricular myocytes.
The present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (OA), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. Acute application of free OA caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mV from a holding potential of -80 mV. The derived 50% inhibitory concentration (IC50) was 12.49+/-0.27 micromol l(-1). At a concentration of 30 micromol l(-1), OA significantly reduced the current density to about 45% of control values, but did not modify either the shape of the current-density voltage relationship or the apparent reversal potential. In addition, OA did not modify the voltage dependence of either steady state inactivation or activation curves. Taken together, these results indicate that physiological concentrations of free OA decrease the conductance of the L-type inward current, without altering its properties of selectivity and its voltage dependence. The inhibitory effect of OA upon the L-type calcium channel may translate, in vivo, into a protective effect against arrhythmias induced by Ca2+ overload.
Chatelier
A
A
Université de La Rochelle, Laboratoire de Biologie et Environnement Marin, Avenue Michel Crépeau, 17042, La Rochelle cedex, France. aurelien.chatelier@crhl.ulaval.ca
Imbert
N
N
Infante
J L Zambonino
JL
McKenzie
D J
DJ
Bois
P
P
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D001464:Barium / Q000378:metabolism*; D001492:Bass / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D019301:Oleic Acid / Q000494:pharmacology*; D018408:Patch-Clamp Techniques
D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D001464:Barium; D019301:Oleic Acid