A cDNA sequence coding for a glutamic acid-rich protein is differentially expressed in cassava storage roots.
We report the isolation and characterization of a cDNA sequence (Mec1) coding for a glutamic acid-rich protein (Pt2L4) from cassava storage roots. Comparative sequence analysis showed a high identity of Pt2L4 with cassava protein C54, which is expressed in vascular tissues of storage roots. Northern blot analysis showed that the Mec1 transcript expression pattern might be related to the maturation of the storage parenchyma cells.
de Souza
Cláudia Regina Batista
CR
EMBRAPA--Recursos Genéticos e Biotecnologia, Caixa Postal 02372, Brasília-DF, 70770-900, Brazil.
Carvalho
Luiz Joaquim Castelo Branco
LJ
de Almeida
Elionor Rita Pereira
ER
Gander
Eugen Silvano
ES
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D018698:Glutamic Acid / Q000235:genetics* / Q000378:metabolism; D002365:Manihot / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D010940:Plant Proteins; D018698:Glutamic Acid