Antifungal effect of amentoflavone derived from Selaginella tamariscina.
Amentoflavone is a plant bif avonoid that was isolated from an ethyl acetate extract of the whole plant of Selaginella tamariscina (Beauv.) spring. 1D and 2D NMR spectroscopy including DEPT, HMQC, and HMBC were used to determine its structure. Amentoflavone exhibited potent antifungal activity against several pathogenic fungal strains but had a very low hemolytic effect on human erythrocytes. In particular, amentoflavone induced the accumulation of intracellular trehalose on C. albicans as a stress response to the drug, and disrupted the dimorphic transition that forms pseudo-hyphae during pathogenesis. In conclusion, amentoflavone has great potential to be a lead compound for the development of antifungal agents.
Jung
Hyun Jun
HJ
Department of Microbiology, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Korea.
Sung
Woo Sang
WS
Yeo
Soo-Hwan
SH
Kim
Hyun Soo
HS
Lee
In-Seon
IS
Woo
Eun-Rhan
ER
Lee
Dong Gun
DG
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D044946:Biflavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D015169:Colony Count, Microbial; D004912:Erythrocytes / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D032503:Selaginellaceae / Q000737:chemistry*; D014199:Trehalose / Q000378:metabolism
D000935:Antifungal Agents; D044946:Biflavonoids; D000666:Amphotericin B; C011164:amentoflavone; D014199:Trehalose