title: Heart slice NMR.
abstract: Nuclear magnetic resonance (NMR) spectroscopy of the heart is normally carried out using whole heart preparations under coronary perfusion. In such preparations, either radical changes in ionic composition of the perfusate or applications of numerous drugs would affect coronary microcirculation. This report communicates the first (31)P NMR spectroscopy study using a heart slice preparation (left ventricular slices) superfused with extracellular medium. The ratio of phosphocreatine concentration to ATP concentration was approximately 2.1. Also, intracellular pH and Mg(2+) concentration ([Mg(2+)](i)), estimated from the chemical shifts of inorganic phosphate and ATP, were comparable with those under retrograde perfusion. [Mg(2+)](i) was significantly increased by the removal of extracellular Na(+), supporting the essential role of Na(+)-coupled Mg(2+) transport in Mg(2+) homeostasis of the heart. Heart slice preparation could also be used to evaluate the potency of cardiac drugs, regardless of their possible effects on coronary microcirculation.
authors.affiliation: Department of Cell Physiology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Uetani
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamashita
authors.forename: Juichiro
authors.initials: J
authors.lastname: Shimizu
authors.forename: Hiromi
authors.initials: H
authors.lastname: Misawa
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tatematsu
authors.forename: Yukihisa
authors.initials: Y
authors.lastname: Hamaguchi
authors.forename: Takehiro
authors.initials: T
authors.lastname: Miyasaka
authors.forename: Yuki
authors.initials: Y
authors.lastname: Katanosaka
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kato
authors.forename: Tatsuaki
authors.initials: T
authors.lastname: Matsubara
authors.forename: Koichi
authors.initials: K
authors.lastname: Furukawa
authors.forename: Toyoaki
authors.initials: T
authors.lastname: Murohara
authors.forename: Miyako
authors.initials: M
authors.lastname: Takaki
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Nakayama
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017920:Antiporters / Q000378:metabolism*; D006352:Heart Ventricles / Q000378:metabolism; D016591:Histocytological Preparation Techniques; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007552:Isotonic Solutions; D008274:Magnesium / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D010725:Phosphocreatine / Q000378:metabolism; D017553:Phosphorus Compounds / Q000378:metabolism; D010759:Phosphorus Isotopes; D051381:Rats; D017208:Rats, Wistar
chemical_list: D017920:Antiporters; D007552:Isotonic Solutions; D017553:Phosphorus Compounds; D010759:Phosphorus Isotopes; C037692:Tyrode's solution; C046250:sodium-magnesium antiporter; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D008274:Magnesium