title: Single-chain antibodies against DNA aptamers for use as adapter molecules on DNA tile arrays in nanoscale materials organization.
abstract: Complex DNA nanostructures have been developed as structural components for the construction of nanoscale objects. Recent advances have enabled self-assembly of organized DNA nanolattices and their use in patterning functional bio-macromolecules and other nanomaterials. Adapter molecules that bind specifically to both DNA lattices and nanomaterials would be useful components in a molecular construction kit for patterned nanodevices. Herein we describe the selection from phage display libraries of single-chain antibodies (scFv) for binding to a specific DNA aptamer and their development as adapter molecules for nanoscale construction. We demonstrate the decoration of various DNA tile structures with aptamers and show binding of the selected single-chain antibody as well as the self-assembly of mixed DNA-protein biomolecular lattices.
authors.affiliation: Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: Hanying
authors.initials: H
authors.lastname: Li
authors.forename: Thomas H
authors.initials: TH
authors.lastname: LaBean
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Kenan
mesh_terms: D000906:Antibodies / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001435:Bacteriophages; D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D007135:Immunoglobulin Variable Region / Q000276:immunology; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods*; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry*; D019151:Peptide Library; D052156:SELEX Aptamer Technique
chemical_list: D000906:Antibodies; D052157:Aptamers, Nucleotide; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D004247:DNA