title: Analysis of TGF-beta protein expression in aggressive fibromatosis (desmoid tumor).
abstract: Aggressive fibromatosis, usually termed desmoid tumor, develops from muscle connective tissue, fasciae and aponeuroses. Aggressive fibromatosis located in various parts of the body demonstrates differentiated biological behavior. Abnormalities in TGF-beta expression are very common in many disease processes, including neoplasms. Immunohistochemical analysis employing a monoclonal antibody against TGF-beta was performed on archival material, consisting of 38 cases of aggressive fibromatosis, among which 23 represented abdominal, 11 extra-abdominal and 4 intra-abdominal localizations. The sections for immunohistochemical study were stained using the streptavidin-biotin (ABC) method. The average percentage of cells positively stained for TGF-beta protein was 40.2% in the group of extra-abdominal, 58.5% in the group of abdominal and 72.8% in the group of intra-abdominal localizations. There were significant differences observed between the analyzed groups of desmoid tumor (p<0.05). A positive cytoplasmic reaction for TGF-beta was noted in 65.8% (25/38) of the aggressive fibromatoses. Overexpression of TGF-beta protein was noted in 39.5% (15/38) of the aggressive fibromatoses. High expression noticed in desmoid fibroblasts might indicate that this protein plays a crucial role in the development of aggressive fibromatosis.
authors.affiliation: Department of Biology and Genetics, Medical University, Lódź.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Ferenc
authors.forename: Liliana
authors.initials: L
authors.lastname: Stalińska
authors.forename: Maria
authors.initials: M
authors.lastname: Turant
authors.forename: Jacek
authors.initials: J
authors.lastname: Sygut
authors.forename: Dariusz
authors.initials: D
authors.lastname: Tosik
authors.forename: Adam
authors.initials: A
authors.lastname: Dziki
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kulig
mesh_terms: D000008:Abdominal Neoplasms / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism; D002452:Cell Count; D003593:Cytoplasm / Q000378:metabolism / Q000473:pathology; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D018222:Desmoid Tumors / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D016212:Transforming Growth Factor beta