Optimization of nutrient parameters for lovastatin production by Monascus purpureus MTCC 369 under submerged fermentation using response surface methodology.
Lovastatin, an inhibitor of HMG-CoA reductase, was produced by submerged fermentation using Monascus purpureus MTCC 369. Five nutritional parameters screened using Plackett-Burman experimental design were optimized by Box-Behnken factorial design of response surface methodology for lovastatin production in shake flask cultures. Maximum lovastatin production of 351 mg/l were predicted in medium containing 29.59 g/l dextrose, 3.86 g/l NH4Cl, 1.73 g/l KH2PO4, 0.86 g/l MgSO4 x 7H2O, and 0.19 g/l MnSO4 x H2O using response surface plots and point prediction tool of DESIGN EXPERT 7.0 (Statease, USA) software.
Sayyad
Sadik Ali
SA
Pharmaceutical Biotechnology Laboratory, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi, 110062, India.
Panda
Bibhu Prasad
BP
Javed
Saleem
S
Ali
Mohd
M
D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D008148:Lovastatin / Q000096:biosynthesis*; D032741:Monascus / Q000378:metabolism*
D003470:Culture Media; D008148:Lovastatin