Protein chip fabrication by capture of nascent polypeptides.
The most challenging step in protein microarray fabrication is high-throughput production of proteins. Here we report two similar strategies to fabricate protein chips through capture onto a solid surface of the nascent polypeptides during translation of synthetic or in vitro-transcribed RNAs. Using these approaches, we efficiently fabricated both peptide and protein microarrays at relatively high density. We further demonstrated that such protein chips can be used to analyze protein activity.
Tao
Sheng-Ce
SC
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Zhu
Heng
H
D002474:Cell-Free System; D000939:Epitopes; D009842:Oligopeptides; D010455:Peptides / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D040081:Protein Array Analysis / Q000379:methods*; D014176:Protein Biosynthesis; D012313:RNA / Q000138:chemical synthesis
D000939:Epitopes; D009842:Oligopeptides; D010455:Peptides; D012313:RNA; C085706:FLAG peptide