Various domains of the B-cell regulatory molecule CD72 has diverged at different rates in mammals: cloning, transcription and mapping of porcine CD72.
We report the cloning of the porcine B-cell co-receptor CD72, as well as genomic mapping and examination of transcription. The B-cell receptor (BCR) complex mediates signalling upon antigen recognition by the membrane bound BCR. Several co-receptors modulate this signal positively or negatively. CD72 has been shown to be a negatively regulating BCR co-receptor. We isolated and sequenced three porcine CD72 transcript variants. Using a pig radiation hybrid panel we found the porcine CD72 gene to be located on chromosome 1q21-28 in a region syntenic to human chromosome 9. The porcine CD72 gene is highly transcribed in lymph node, thymus and lung tissues as well as in pulmonary alveolar macrophages. The predicted porcine CD72 polypeptide shows conservation of immunoreceptor tyrosine-based inhibitory motifs and an extracellular C-type lectin domain. Compared to CD72 sequences from other mammals as well as from chicken, the polypeptide is highly conserved in the intracellular part and much less conserved in the extracellular part. We suggest that this difference might be due to the different nature of ligands and the constrains on these to co-evolve.
Bie Petersen
Cathrine
C
Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Denmark. cbp@kvl.dk
Nygård
Ann-Britt
AB
Fredholm
Merete
M
Aasted
Bent
B
Salomonsen
Jan
J
D017398:Alternative Splicing; D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D033481:Chromosomes, Mammalian / Q000235:genetics; D003001:Cloning, Molecular; D005260:Female; D020869:Gene Expression Profiling; D014644:Genetic Variation; D006801:Humans; D008168:Lung / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000378:metabolism; D008297:Male; D008322:Mammals / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics; D021101:Radiation Hybrid Mapping; D016415:Sequence Alignment; D034421:Sus scrofa / Q000235:genetics*; D026801:Synteny; D013950:Thymus Gland / Q000378:metabolism; D014158:Transcription, Genetic
D000944:Antigens, Differentiation, B-Lymphocyte; D020033:Protein Isoforms