title: Human islet isolation outcomes from pancreata preserved with Histidine-Tryptophan Ketoglutarate versus University of Wisconsin solution.
abstract: This study was designed to compare Histadine-Tryptophan-Ketogluterate (HTK) with University of Wisconsin (UW) solution. Pancreata from extended criteria donors were flushed and transported with HTK (n=41) or UW (n=45). Isolation outcomes were determined by islet yields, viability and in vitro and in vivo function. Final yields were similar between two groups (HTK: 383,085 vs. UW: 328,514 EIN, P=0.14). In the HTK group, 63.4% (26/41) of isolations resulted in a yield of over 300,000, and in the UW group this was achieved in 46.7% (21/45; P=0.12). Viability results were similar (HTK: 82.9 vs. UW: 82.7%, P=0.93). Stimulation index in the HTK and UW groups were comparable (5.28 vs. 4.91, P=0.62). Ten out of 41 islet preparations in HTK and 4 of 45 in UW group were suitable for clinical transplantation (P=0.05). Our study shows HTK is equivalent to UW solution in the preservation of pancreata for islet isolation.
authors.affiliation: Department of Surgery, Division of Transplantation/Islet Transplantation Program, University of Illinois Medical Center at Chicago, IL 60612, USA.
authors.forename: Payam
authors.initials: P
authors.lastname: Salehi
authors.forename: Michael A
authors.initials: MA
authors.lastname: Hansen
authors.forename: Jose G
authors.initials: JG
authors.lastname: Avila
authors.forename: Barbara
authors.initials: B
authors.lastname: Barbaro
authors.forename: Antonio
authors.initials: A
authors.lastname: Gangemi
authors.forename: Travis
authors.initials: T
authors.lastname: Romagnoli
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Meirigeng
authors.initials: M
authors.lastname: Qi
authors.forename: Philip
authors.initials: P
authors.lastname: Murdock
authors.forename: Enrico
authors.initials: E
authors.lastname: Benedetti
authors.forename: Jose
authors.initials: J
authors.lastname: Oberholzer
mesh_terms: D000241:Adenosine; D000328:Adult; D000493:Allopurinol; D005260:Female; D005947:Glucose; D005978:Glutathione; D006801:Humans; D007328:Insulin; D007515:Islets of Langerhans / Q000166:cytology*; D008297:Male; D008353:Mannitol; D008875:Middle Aged; D019188:Organ Preservation Solutions; D010179:Pancreas / Q000166:cytology*; D011189:Potassium Chloride; D011343:Procaine; D011887:Raffinose; D014019:Tissue Donors / Q000706:statistics & numerical data
chemical_list: C039984:Bretschneider cardioplegic solution; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D011343:Procaine; D000493:Allopurinol; D011189:Potassium Chloride; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose