title: Improvement of carbonyl reductase production of Geotrichum candidum for the transformation of 1-acetonaphthone to S(-)-1-(1'-napthyl) ethanol.
abstract: The aim of this work was to study the influence of media components and physico-chemical parameters on the growth and carbonyl reductase production by Geotrichum candidum. Under optimized conditions, the conversion of the substrate increased to >93%, while the specific growth rate and enzyme activity were increased by 200% and 29%, respectively. The rate of conversion of the substrate was also very high in the cells grown in optimized medium. The volumetric productivity of the biotransformation process was much higher (0.27g/lh) with the cells grown in the optimized medium compared to that of grown in un-optimized medium (0.16g/lh). The cells were also highly stable in the operational condition, indicating the feasibility of their use in multiple batches of reaction.
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S Nagar, Punjab 160 062, India.
authors.forename: Mani Shankar
authors.initials: MS
authors.lastname: Bhattacharyya
authors.forename: U C
authors.initials: UC
authors.lastname: Banerjee
mesh_terms: D001711:Biotransformation; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D000431:Ethanol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D005848:Geotrichum / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009281:Naphthalenes / Q000737:chemistry / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D013696:Temperature
chemical_list: C521161:1-(1'-naphthyl)ethanol; D003470:Culture Media; D009281:Naphthalenes; D000431:Ethanol; D002244:Carbon; D009584:Nitrogen; C521160:1-acetonaphthone