[Relationship between MC4R gene polymorphyism and body weight trait in beagle dogs].
The relationship between MC4R gene polymorphism and body weight in beagle dogs was analyzed. Using gene-specific primers based on canine MC4R exonic sequences a gene fragment was PCR amplified, cloned and sequenced to identify potential polymorphisms. The relationship between a MC4R gene polymorphism detected by PCR-RFLP and canine body weight was analyzed. Three variants were found in beagle dog MC4R DNA sequence, of which two were deletions and one was a transversion which created a PshA I site that could be detected by PCR-RFLP. A statistically significant relationship between this polymorphism and body weight was found. MC4R gene could be a candidate modifier gene for canine body weight.
Zhang
Yi-Bo
YB
Department of Immunology and Pathogenic Biology, Jinzhou Medical College, Jinzhou 121001, China. jyzyb@yahoo.com.cn
Ba
Cai-Feng
CF
Su
Yu-Hong
YH
Zeng
Rui-Xia
RX
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics*; D004285:Dogs / Q000235:genetics* / Q000502:physiology*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D044105:Receptor, Melanocortin, Type 4 / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
D044105:Receptor, Melanocortin, Type 4