[The theory and practice of constructing calibration curves in biodozimetry].
The methodical peculiarities of experimental construction of regression "dose-effect" relationships used for the dose reconstruction are discussed. The method of computer simulations is applied to study the efficiency of different statistical procedures for plotting regression curves as well as the dependence of errors in dose prediction on the volume of examined material and on the choice of doses for a calibration curve. The causes of essential variability of calibrations obtained by different teams of researchers are discussed. A number of methodical recommendations is given for statistical processing of cytogenetic data. The procedure of constructing calibration dose dependence of the frequency of dicentrics on the basis experiments with in vitro gamma-irradiation of lymphocytes from blood samples of 5 donors is considered in detail. The expressions for statistical errors occurring in the dose reconstruction made on the base of the frequency of aberrations were derived and checked by the computer experiment.
Rubanovich
A V
AV
Snegireva
G P
GP
Shevchenko
Akaeva
E A
EA
Elisova
T V
TV
Kuznetsova
G I
GI
Nilova
I N
IN
Iofa
E L
EL
D002138:Calibration; D003198:Computer Simulation; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects; D011874:Radiometry / Q000379:methods* / Q000592:standards*