[A study on expression of HIF-1alpha and EPO in the hippocampus of rats with vascular dementia].
OBJECTIVE
To observe the expression rule of hypoxia inducible factor-1 (HIF-1alpha) and erythropoietion (EPO) in the formation of vascular dementia (VD) and investigate the possible pathogenesis of VD.


METHODS
Rats of experimental group were treated with a permanent bilateral common carotid arteries (CCA) occlusion (2-VO) for establishing vascular dementia model. Rats were evaluated on learning-memory ability by Y-type water maze test. The dynamic expression of HIF-1alpha and EPO in hippocampal CA1 region were measured by immunohistochemical assay method.


RESULTS
(1) The learning-memory ability of rats in VD groups was progressively decreased as the ischemic duration prolonged (P < 0.05); (2) In VD group, the expression of HIF-1alpha and EPO in hippocampal CA1 region were most obvious at 1 w, and then declined progressively but still above the normal level (P < 0.01); (3) In VD group, the expression of HIF-1alpha and EPO at each ischemic point and their corresponding learning-memory ability were in significant correlation at the 0.01 level.


CONCLUSIONS
Both HIF-1alpha and EPO contribute to the formation of VD, and HIF-1/EPO anoxic signal transduction may play a protecting role in this process.
Zhang
Bei
B
Department of Neurology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
Wu
Hai-qin
HQ
Zhang
Hai-xiong
HX
Zhang
Gui-lian
GL
Zhan
Shu-qin
SQ
D000818:Animals; D015140:Dementia, Vascular / Q000378:metabolism* / Q000503:physiopathology; D004921:Erythropoietin / Q000096:biosynthesis* / Q000235:genetics; D006624:Hippocampus / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D018782:Maze Learning; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D004921:Erythropoietin