Probing the stability of biocompatible sodium hyaluronate/chitosan nanocoatings against changes in salinity and pH.
The stability of polyelectrolyte multilayer assemblies was investigated with emphasis on the effects of solution ionic strength, pH, and polymer molecular weight on the film thickness and surface topography. The multilayers consisting of two polysaccharides, the polyanion sodium hyaluronate (HA) and the polycation chitosan (CH) were studied using surface plasmon resonance (SPR) spectroscopy, impedance quartz crystal microbalance (QCM), and atomic force microscopy (AFM). SPR/QCM experiments show that coatings consisting of four HA/CH bilayers assembled at pH 4.5 in the presence of 0.15 M NaCl are stable in NaCl solutions of concentration less than 0.8 M. These multilayers are stable when placed in contact with aqueous solutions ranging in pH from 3.5 to 9. The molecular weight of the polysaccharides has only a marginal effect on the stability of the films in the range explored here (HA: Mn = 360,000 or 31,000 g/mol; CH: Mn = 160,000 or 30,000 g/mol). AFM imaging reveals that different mechanisms may account for the multilayers stability versus salt and pH treatments. While increasing the ionic strength induces reorganization of the surface topography from isolated spherical islets to elongated worm-like features, changes in pH have no appreciable effects on the coating topography prior to complete disintegration.
Kujawa
Piotr
P
Faculté de Pharmacie, Université de Montréal, C. P. 6128, Succursale Centre-Ville, Montréal QC, Canada.
Sanchez
Jacqueline
J
Badia
Antonella
A
Winnik
Françoise M
FM
D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004355:Drug Stability; D017097:Electric Impedance; D006820:Hyaluronic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*; D009994:Osmolar Concentration; D012965:Sodium Chloride; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D012965:Sodium Chloride; D006820:Hyaluronic Acid; D048271:Chitosan