title: Differential effects of water deprivation and rehydration on Fos and FosB/DeltaFosB staining in the rat brainstem.
abstract: This study examined the effects of dehydration and rehydration with water on Fos and FosB staining in the brainstem of rats. Male rats were water deprived for 48 h (Dehyd, n=7) or 46 h followed by 2 h access to water (Rehyd, n=7). Controls had ad libitum access to water (Con, n=9). Brainstems were stained for Fos and FosB/DeltaFosB using commercially available antibodies. In the nucleus of the solitary tract (NTS), the number of Fos stained neurons was significantly increased by dehydration and increased further following rehydration (Con 5+/-1; Dehyd 22+/-1; Rehyd 48+/-5). The average number of Fos-positive cells in the parabrachial nucleus (PBN) was significantly increased only by rehydration (Con 12+/-2; Dehyd 6+/-2; Rehyd 51+/-4). The area postrema (AP) showed significant increases in Fos staining after dehydration and rehydration (Fos: Con 4+/-1; Dehyd 28+/-3; Rehyd 24+/-3). In the rostral ventrolateral medulla (RVL), Fos staining significantly increased after dehydration and this effect was reduced by rehydration (Con 3+/-1; Dehyd 21+/-2; Rehyd 12+/-1). In contrast, Fos staining in the caudal ventrolateral medulla (CVL) was not significantly influenced following either dehydration or rehydration with water (Con 4+/-1; Dehyd 4+/-1; Rehyd 5+/-1). FosB/DeltaFosB staining in the NTS, AP, and RVL was comparably increased by dehydration and rehydration. In the PBN and CVL, FosB/DeltaFosB staining was not affected by the treatments. Dehydration and rehydration have regionally specific effects on Fos and FosB/DeltaFosB staining in the brainstem.
authors.affiliation: Department of Pharmacology, and the Center for Biomedical Neuroscience, UTHSCSA, USA.
authors.forename: Lisa L
authors.initials: LL
authors.lastname: Ji
authors.forename: Helmut B
authors.initials: HB
authors.lastname: Gottlieb
authors.forename: Maurice L
authors.initials: ML
authors.lastname: Penny
authors.forename: Tiffany
authors.initials: T
authors.lastname: Fleming
authors.forename: Glenn M
authors.initials: GM
authors.lastname: Toney
authors.forename: J Thomas
authors.initials: JT
authors.lastname: Cunningham
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D001933:Brain Stem / Q000378:metabolism* / Q000502:physiology*; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D005440:Fluid Therapy; D016762:Genes, fos / Q000502:physiology*; D006400:Hematocrit; D007150:Immunohistochemistry; D008297:Male; D009994:Osmolar Concentration; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014868:Water Deprivation / Q000502:physiology*
chemical_list: D001798:Blood Proteins; C483820:Fosb protein, rat; D016760:Proto-Oncogene Proteins c-fos; D004299:Dopamine beta-Hydroxylase