Maternal IgG suppresses NMDA-induced spasms in infant rats and inhibits NMDA-mediated neurotoxicity in hippocampal neurons.
Maternal immunoglobulin G (IgG) was derived from Wistar rats that just delivered the new offsprings. We examined the effect of this maternal IgG on infantile spasms induced by N-methyl-d-aspartate (NMDA) in immature rats. Pup animals were treated subcutaneously with 10 mg/kg/day maternal IgG from day 11 to day 15 after birth followed by a single intraperitoneal dose of NMDA (15 mg/kg). Administration of maternal IgG decreased the severity and increased the number of ACTH immunoreactive cells in the cortex of rats with NMDA-induced spasms. Furthermore, maternal IgG inhibited NMDA-induced intracellular LDH activity in cultured hippocampal neurons in a dose-dependent manner. The results indicate that maternal IgG can attenuate NMDA-induced seizures. In infantile spasms, some factors may during pregnancy negatively affect the transfer of maternal IgG from mother to fetus thereby causing a decrease in the amount of protective maternal IgG.
Zou
Li-Ping
LP
Department of Neurology, Beijing Children's Hospital, The Capital University of Medical Sciences, Beijing 100045, China. zouliping21@hotmail.com
Zhang
Wei-Hua
WH
Wang
Hong-Mei
HM
Zen
Min
M
Chen
Kesi
K
Mix
Eilhard
E
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D000833:Animals, Suckling; D002478:Cells, Cultured; D004195:Disease Models, Animal; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity; D005260:Female; D006624:Hippocampus / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism* / Q000494:pharmacology; D007167:Immunotherapy / Q000379:methods; D007231:Infant, Newborn; D008297:Male; D008892:Milk / Q000276:immunology* / Q000378:metabolism; D016202:N-Methylaspartate / Q000633:toxicity; D009474:Neurons / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013036:Spasms, Infantile / Q000139:chemically induced / Q000276:immunology* / Q000628:therapy*
D018690:Excitatory Amino Acid Agonists; D007074:Immunoglobulin G; D016202:N-Methylaspartate; D000324:Adrenocorticotropic Hormone