Improvement of carbonyl reductase production of Geotrichum candidum for the transformation of 1-acetonaphthone to S(-)-1-(1'-napthyl) ethanol.
The aim of this work was to study the influence of media components and physico-chemical parameters on the growth and carbonyl reductase production by Geotrichum candidum. Under optimized conditions, the conversion of the substrate increased to >93%, while the specific growth rate and enzyme activity were increased by 200% and 29%, respectively. The rate of conversion of the substrate was also very high in the cells grown in optimized medium. The volumetric productivity of the biotransformation process was much higher (0.27g/lh) with the cells grown in the optimized medium compared to that of grown in un-optimized medium (0.16g/lh). The cells were also highly stable in the operational condition, indicating the feasibility of their use in multiple batches of reaction.
Bhattacharyya
Mani Shankar
MS
Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S Nagar, Punjab 160 062, India.
Banerjee
U C
UC
D001711:Biotransformation; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D000431:Ethanol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D005848:Geotrichum / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009281:Naphthalenes / Q000737:chemistry / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D013696:Temperature
C521161:1-(1'-naphthyl)ethanol; D003470:Culture Media; D009281:Naphthalenes; D000431:Ethanol; D002244:Carbon; D009584:Nitrogen; C521160:1-acetonaphthone