Single-chain antibodies against DNA aptamers for use as adapter molecules on DNA tile arrays in nanoscale materials organization.
Complex DNA nanostructures have been developed as structural components for the construction of nanoscale objects. Recent advances have enabled self-assembly of organized DNA nanolattices and their use in patterning functional bio-macromolecules and other nanomaterials. Adapter molecules that bind specifically to both DNA lattices and nanomaterials would be useful components in a molecular construction kit for patterned nanodevices. Herein we describe the selection from phage display libraries of single-chain antibodies (scFv) for binding to a specific DNA aptamer and their development as adapter molecules for nanoscale construction. We demonstrate the decoration of various DNA tile structures with aptamers and show binding of the selected single-chain antibody as well as the self-assembly of mixed DNA-protein biomolecular lattices.
Li
Hanying
H
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA.
LaBean
Thomas H
TH
Kenan
Daniel J
DJ
D000906:Antibodies / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001435:Bacteriophages; D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D007135:Immunoglobulin Variable Region / Q000276:immunology; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods*; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry*; D019151:Peptide Library; D052156:SELEX Aptamer Technique
D000906:Antibodies; D052157:Aptamers, Nucleotide; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D004247:DNA