title: CD8alpha+ dendritic cells are required for efficient entry of Listeria monocytogenes into the spleen.
abstract: In addition to their bridging function between innate and adaptive immunity, dendritic cells (DCs) may also contribute to primary resistance against infection. Here we analyzed the role of DCs during infection with Listeria monocytogenes by performing systemic in vivo depletion of these cells. We showed that CD8alpha(+) DCs were crucial for L. monocytogenes spreading and proliferation in the spleen. Efficient and rapid uptake of L. monocytogenes by CD8alpha(+) DCs required the small GTPase Rac1 and is a general characteristic of this DC subpopulation in filtering particles out of the blood. Thus, CD8alpha(+) DCs appear to play an important role for efficient bacterial entry into the spleen, which is of relevance for subsequent immune responses.
authors.affiliation: Institute for Medical Microbiology, Immunology, and Hygiene, Technical University Munich, 81675 Munich, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Neuenhahn
authors.forename: Kristen M
authors.initials: KM
authors.lastname: Kerksiek
authors.forename: Magdalena
authors.initials: M
authors.lastname: Nauerth
authors.forename: Michael H
authors.initials: MH
authors.lastname: Suhre
authors.forename: Matthias
authors.initials: M
authors.lastname: Schiemann
authors.forename: Friedemann E
authors.initials: FE
authors.lastname: Gebhardt
authors.forename: Christian
authors.initials: C
authors.lastname: Stemberger
authors.forename: Klaus
authors.initials: K
authors.lastname: Panthel
authors.forename: Samira
authors.initials: S
authors.lastname: Schröder
authors.forename: Trinad
authors.initials: T
authors.lastname: Chakraborty
authors.forename: Steffen
authors.initials: S
authors.lastname: Jung
authors.forename: Hubertus
authors.initials: H
authors.lastname: Hochrein
authors.forename: Holger
authors.initials: H
authors.lastname: Rüssmann
authors.forename: Thomas
authors.initials: T
authors.lastname: Brocker
authors.forename: Dirk H
authors.initials: DH
authors.lastname: Busch
mesh_terms: D000818:Animals; D039521:CD11c Antigen / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis*; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology / Q000637:transplantation; D006098:Granulocytes / Q000276:immunology; D008089:Listeria monocytogenes; D008088:Listeriosis / Q000201:enzymology / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000382:microbiology*; D013601:T-Lymphocytes / Q000276:immunology; D020744:rac GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D020830:rac1 GTP-Binding Protein
chemical_list: D039521:CD11c Antigen; D016827:CD8 Antigens; C431698:CD8alpha antigen; D009479:Neuropeptides; C484602:Rac1 protein, mouse; D020744:rac GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein