[Research into the human genome driven by improved methods].
The enormous progress made by research of the human genome is mainly driven by newly established or improved methods for the analysis of nucleic acids and proteins. Among the methods that have gained a wide-spread use within a comparably short time are fluorescence in situ hybridization (FISH), polymerase chain reaction (PCR) including methods for quantitative PCR, and the use of short interfering RNA (siRNA) molecules aimed at gene silencing. The increasing significance of the analysis of secondary modifications of nucleic acids and proteins (genomic imprinting by DNA methylation, posttranslational protein modification) is reflected by an increasing use of mass spectrometry for the analysis and characterization of these biomolecules. Overall, in the future the research into the human genome and the interpretation of data will further benefit from these and other refined tools.
Bullerdiek
J
J
Zentrum für Humangenetik, Universität Bremen, Leobener Strasse ZHG, 28359 Bremen, BRD. bullerd@uni-bremen.de
D000818:Animals; D002478:Cells, Cultured; D019175:DNA Methylation; D004195:Disease Models, Animal; D005544:Forecasting; D020868:Gene Silencing; D036281:Genetic Research; D015894:Genome, Human; D018392:Genomic Imprinting; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D013058:Mass Spectrometry; D008495:Medical Oncology / Q000379:methods*; D051379:Mice; D018345:Mice, Knockout; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction