[Identification and structural analysis of a glycophospholipid component from the venom of ant Paraponera clavata].
The venom of South American ant Paraponera clavata and its low-molecular-mass fraction were shown to possess insectotoxic and pore-forming activities. A number of glycophospholipid components were isolated from this ant venom by means of gel filtration and reversed-phase chromatography. Some of the compounds cause conductivity fluctuations in lipid bilayer membranes within the ranges 3-25 pS and 200-400 pS at concentrations of 10(-6) to 10(-7) M. N-Acetylglucosamine, a fatty acid, and phosphoric acid residues were found in their structures. A full structure, 3-myristoyl-2-acetamido-2-deoxy-alpha-D-glucopyranosyl phosphate, was elucidated for one of the compounds by the use of 1H, 13C, and 31P NMR spectroscopy and mass spectrometry.
Pluzhnikov
K A
KA
Bocharov
D N
DN
Kononova
N V
NV
Sukhanov
S V
SV
Balashova
T A
TA
Arsen'ev
A S
AS
Grishin
E V
EV
D000818:Animals; D000862:Ant Venoms / Q000032:analysis*; D001000:Ants / Q000187:drug effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005958:Glucosephosphates / Q000737:chemistry*; D006017:Glycolipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity
C515414:3-myristoyl-2-acetamido-2-deoxyglucopyranosyl phosphate; D000862:Ant Venoms; D005958:Glucosephosphates; D006017:Glycolipids; D010743:Phospholipids