Quantification and stability of human adenoviruses and polyomavirus JCPyV in wastewater matrices.
Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.
Bofill-Mas
Silvia
S
Department of Microbiology, Faculty of Biology, University of Barcelona, Av. Diagonal 645, Barcelona 08028, Spain, and Department of Clinical Virology, Umeå University Hospital, Sweden.
Albinana-Gimenez
Nestor
N
Clemente-Casares
Pilar
P
Hundesa
Ayalkibet
A
Rodriguez-Manzano
Jesus
J
Allard
Annika
A
Calvo
Miquel
M
Girones
Rosina
R
D000260:Adenoviruses, Human / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000821:virology; D005618:Fresh Water / Q000821:virology; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012722:Sewage / Q000821:virology; D014865:Waste Disposal, Fluid / Q000379:methods*; D014876:Water Pollution / Q000032:analysis*
D012722:Sewage