Expression of a carbonic anhydrase gene is induced by environmental stresses in rice (Oryza sativa L.).
Expression of the gene (OsCA1) coding for carbonic anhydrase (CA) in leaves and roots of rice was induced by environmental stresses from salts (NaCl, NaHCO(3) and Na(2)CO(3)), and osmotic stress (10%, w/v, PEG 6000). CA activity of rice seedlings more than doubled under some of these stresses. Transgenic Arabidopsis over-expressing OsCA1 had a greater salt tolerance at the seedling stage than wild-type plants in 1/2 MS medium with 5 mM NaHCO(3), 50 mM NaCl, on 100 mM NaCl. Thus CA expression responds to environmental stresses and is related to stress tolerance in rice.
Yu
Song
S
Alkali Soil Natural Environmental Science Center, Stress Molecular Biology Laboratory, Northeast Forestry University, Harbin, PR China.
Zhang
Xinxin
X
Guan
Qingjie
Q
Takano
Tetsuo
T
Liu
Shenkui
S
D000595:Amino Acid Sequence; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000378:metabolism*; D004777:Environment; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D012492:Salts / Q000494:pharmacology*
D012492:Salts; D002256:Carbonic Anhydrases