title: Subpollen particles: carriers of allergenic proteins and oxidases.
abstract: BACKGROUND
  Pollen is known to induce allergic asthma in atopic individuals, although only a few inhaled pollen grains penetrate into the lower respiratory tract.
  
  
  OBJECTIVE
  We sought to provide evidence that subpollen particles (SPPs) of respirable size, possessing both antigenic and redox properties, are released from weed pollen grains and to test their role in allergic airway inflammation.
  
  
  METHODS
  The release of SPPs was analyzed by means of microscopic imaging and flow cytometry. The redox properties of SPPs and the SPP-mediated oxidative effect on epithelial cells were determined by using redox-sensitive probes and specific inhibitors. Western blotting and amino acid sequence analysis were used to examine the protein components of the SPP. The allergenic properties of the SPP were determined in a murine model of experimental asthma.
  
  
  RESULTS
  Ragweed pollen grains released 0.5 to 4.5 microm of SPPs on hydration. These contained Amb a 1, along with other allergenic proteins of ragweed pollen, and possessed nicotinamide adenine dinucleotide (reduced) or nicotinamide adenine dinucleotide phosphate (reduced) [NAD(P)H] oxidase activity. The SPPs significantly increased the levels of reactive oxygen species (ROS) in cultured cells and induced allergic airway inflammation in the experimental animals. Pretreatment of the SPPs with NAD(P)H oxidase inhibitors attenuated their capacity to increase ROS levels in the airway epithelial cells and subsequent airway inflammation.
  
  
  CONCLUSIONS
  The allergenic potency of SPPs released from ragweed pollen grains is mediated in tandem by ROS generated by intrinsic NAD(P)H oxidases and antigenic proteins.
  
  
  CONCLUSIONS
  Severe clinical symptoms associated with seasonal asthma might be explained by immune responses to inhaled SPPs carrying allergenic proteins and ROS-producing NAD(P)H oxidases.
authors.affiliation: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX77555, USA.
authors.forename: Attila
authors.initials: A
authors.lastname: Bacsi
authors.forename: Barun K
authors.initials: BK
authors.lastname: Choudhury
authors.forename: Nilesh
authors.initials: N
authors.lastname: Dharajiya
authors.forename: Sanjiv
authors.initials: S
authors.lastname: Sur
authors.forename: Istvan
authors.initials: I
authors.lastname: Boldogh
mesh_terms: D000485:Allergens / Q000276:immunology*; D027721:Amaranthus / Q000276:immunology / Q000648:ultrastructure*; D031164:Ambrosia / Q000276:immunology / Q000648:ultrastructure*; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000276:immunology; D010105:Oxygenases / Q000378:metabolism*; D010316:Particle Size; D011058:Pollen / Q000235:genetics / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000276:immunology*; D020545:Respiratory Mucosa / Q000276:immunology
chemical_list: D000485:Allergens; D017382:Reactive Oxygen Species; D010105:Oxygenases