Cassava (Manihot esculenta Crantz).
During the last three years the generation of stably transformed cassava plants having value-added traits has become a reality. Currently, two Agrobacterium-mediated transformation systems are routinely used to engineer cassava. These systems use either somatic embryos or friable embryogenic calli. This paper presents detailed protocols for the transformation of cassava using primary somatic embryos. The effects of explant types, tissue culture conditions, and bacterial and plasmid related factors on transformation efficiency are discussed.
Msikita
Weston
W
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA.
Ihemere
Uzoma
U
Siritunga
Dimuth
D
Sayre
Richard T
RT
D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D003470:Culture Media; D005818:Genetic Engineering; D018525:Germination; D002365:Manihot / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D040901:Proteomics; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
D003470:Culture Media; D010940:Plant Proteins