Drosophila dCBP is involved in establishing the DNA replication checkpoint.
The CBP/p300 family of proteins comprises related acetyltransferases that coactivate signal-responsive transcription. Recent evidence suggests that p300/CBP may also interact directly with complexes that mediate different aspects of DNA metabolism such as replication and repair. In this report, we show that loss of dCBP in Drosophila cells and eye discs results in a defect in the cell cycle arrest induced by stalled DNA replication. We show that dCBP and the checkpoint kinase Mei-41 can be found together in a complex and, furthermore, that dCBP has a genetic interaction with mei-41 in the response to stalled DNA replication. These observations suggest a broader role for the p300/CBP acetyltransferases in the modulation of chromatin structure and function during DNA metabolic events as well as for transcription.
Smolik
Sarah
S
Oregon Health and Sciences University, NRC3, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA. smoliks@ohsu.edu
Jones
Kristen
K
D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry*; D004260:DNA Repair; D004261:DNA Replication; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster; D016678:Genome; D006579:Heterozygote; D006720:Homozygote; D008957:Models, Genetic; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D034741:RNA, Small Interfering; D016196:S Phase; D015398:Signal Transduction; D014158:Transcription, Genetic; D050880:p300-CBP Transcription Factors / Q000378:metabolism / Q000502:physiology*
D018797:Cell Cycle Proteins; D002843:Chromatin; D029721:Drosophila Proteins; D034741:RNA, Small Interfering; D050880:p300-CBP Transcription Factors; C444435:Mei-41 protein, Drosophila; D017346:Protein Serine-Threonine Kinases