title: Sodium metavanadate affected control and streptozotocin-diabetic rat liver golgi complexes.
abstract: As we have observed previously, rat liver Golgi complexes are very useful cell organelle in investigating the effectiveness of various drugs with cytoprotective or normalizing activities in streptozotocin (STZ)-induced diabetes. The diabetes-associated biochemical and morphological alterations in the organelle were investigated in four groups of rats: control and STZ-diabetic (C and D groups) and compared with the same groups treated with 1.5 mM metavanadate administered as drinking solutions in 0.09 M NaCl over 7 days (C+V and D+V groups). Apart from the untreated group C, a decrease of body weight during the experiment was noted in the remaining three groups, reaching a statistically significant level in the diabetic groups (c. 15%). Fluid and food intake were statistically significantly (p<0.001) limited in both the vanadium treated groups. In the diabetic group treated with metavanadate, the free blood sugar level decreased, but euglycemia was not achieved. In groups C+V, D and D+V, the activity of Golgi marker enzyme, i.e. galactosyltransferase (GalT), was statistically lower as compared with group C (p<0.001). The treatment of diabetic rats with 1.5 mM NaVO3 [V(V)I in 0.09 M NaCl as a drinking solution during 7 days did not normalize the yield of Golgi membrane preparations or the Golgi marker enzyme activity. Electron microscopy revealed marked ultrastructural changes triggered by the employed vanadium compound. A striking change was seen in the presence of giant intracytoplasmic vacuoles. These alterations were seen in both experimental groups, i.e. C+V and D+V. Group C+V showed more advanced ultrastructural changes, what was expressed in a poorer state of mitochondrial membranes, a greater number of vesicular structures and less frequently seen Golgi structures. In spite of the fact that the animals were exposed to two compounds with a strong biological activity, the group of diabetic rats treated with metavanadate (D+V) showed no such advanced changes: more numerous Golgi structures were noted and their form was practically ring-like, i.e. characteristic for this group of organelles.
authors.affiliation: Department of Pathomorphology, Jagiellonian University Collegium Medicum, Kraków. mpdabros@cyf-kr.edu.pl
authors.forename: Wojciech
authors.initials: W
authors.lastname: Dabroś
authors.forename: Anna
authors.initials: A
authors.lastname: Goc
authors.forename: Bohdan
authors.initials: B
authors.lastname: Turyna
authors.forename: Anna M
authors.initials: AM
authors.lastname: Kordowiak
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy / Q000473:pathology*; D004326:Drinking / Q000187:drug effects; D004336:Drug Antagonism; D004435:Eating / Q000187:drug effects; D005260:Female; D005700:Galactosyltransferases / Q000378:metabolism; D006056:Golgi Apparatus / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D007004:Hypoglycemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D008099:Liver / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014638:Vanadates / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D014638:Vanadates; D005700:Galactosyltransferases