Unraveling the spatial distribution of immunoglobulins, enzymes, and polyelectrolytes within layer-by-layer self-assembled multilayers. Ellipsometric studies.
The ellipsometric characterization of a layer-by-layer electrostatically self-assembled multilayer of polyphenol oxidase and alkaline phosphatase with the polycation poly(dimethyldiallylammonium chloride) built on an immunologic layer formed by immunoglobulin G (IgG) and glucose oxidase-conjugated anti-IgG (IgG-GOD) on glassy carbon is reported. The step-by-step evolution of the psi-Delta ellipsometric angles was followed during film growth. Two optical models, named the three-layer film model and reorganization film model, were employed and found suitable for ellipsometric data interpretation. A comparative analysis of film optical properties, film thickness, and ellipsometric mass assessed from both models is also presented.
Ferreyra
N F
NF
INFIQC-Departamento de Físicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, Argentina. ferreyra@mail.fcq.unc.edu.ar
Forzani
E S
ES
López Teijelo
M
M
Coche-Guérente
L
L
Labbé
P
P
D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000378:metabolism; D003198:Computer Simulation; D004573:Electrolytes / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology; D008954:Models, Biological
D004573:Electrolytes; D007074:Immunoglobulin G; D005949:Glucose Oxidase; D000469:Alkaline Phosphatase