A rapid HPLC/ESI-MS method for the quantitative determination of oridonin in rat plasma.
A rapid and accurate method using liquid chromatography with electrospray ionization mass spectrometric detection (HPLC/ESI-MS) was developed and validated for the determination of oridonin in rat plasma. The analytes were extracted with ethyl acetate-n-butyl alcohol (100:2, v/v) after spiking the samples with ethyl hydroxybenzoate (internal standard). The separation was carried out on a Diamon-sil C18 column with an isocratic mobile phase consisting of methanol-water (80:20, v/v) at a flow rate of 1.0 ml/min. The lower limit of quantification (LLOQ) of the method was 10 ng/ml and the linear range was 10-4000 ng/ml. The intra-day and inter-day accuracy and precision of the assay were less than 9%. This method has been applied successfully to a preliminary pharmacokinetic study involving the intravenous administration of oridonin to rats.
Xu
Wen
W
Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, PR China.
Sun
Jin
J
Zhang
Tingting
T
Tang
Jingling
J
Li
Haiyan
H
Fang
Jinling
J
Chen
Dawei
D
He
Zhonggui
Z
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000097:blood* / Q000493:pharmacokinetics; D045786:Diterpenes, Kaurane; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D019999:Pharmaceutical Solutions; D011786:Quality Control; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D045786:Diterpenes, Kaurane; D019999:Pharmaceutical Solutions; C011959:oridonin