Starkeya koreensis sp. nov., isolated from rice straw.
A facultatively chemolithoautotrophic, Gram-negative, aerobic, highly curved rod-shaped, non-motile, non-spore-forming bacterium, strain Jip08(T), was isolated from rice straw in Daejeon, South Korea. Strain Jip08(T) produced colourless colonies (1.0-1.5 mm) on R2A medium after 3 days. On the basis of 16S rRNA gene sequence similarity, strain Jip08(T) was shown to belong to the alpha-2 subclass of the Proteobacteria, and was most closely related to Starkeya novella IAM 12100(T) (98.6 %), Angulomicrobium tetraedale DSM 5895(T) (98.1 %), Angulomicrobium amanitiforme NCIMB 1785(T) (98.0 %), Ancylobacter aquaticus ATCC 25396(T) (96.6 %) and Methylorhabdus multivorans DM13(T) (95.8 %), all of which belong to the family Hyphomicrobiaceae. The genomic DNA G+C content of strain Jip08(T) was 69 mol%. Chemotaxonomic data [major ubiquinone, Q-10; major fatty acids, C(18 : 1) isomer (summed feature of C(18 : 1)omega7c/omega9t/omega12t), C(19 : 0) cyclo omega8c and C(16 : 0)], 16S rRNA gene sequence analyses and some morphological and physiological features supported the affiliation of strain Jip08(T) to the genus Starkeya. However, DNA-DNA hybridization data and some phenotypic properties showed that strain Jip08(T) could be distinguished from the single known Starkeya species and represented a novel species, for which the name Starkeya koreensis sp. nov. is proposed. The type strain is Jip08(T) (=KCTC 12212(T)=NBRC 100963(T)=IAM 15215(T)).
Im
Wan-Taek
WT
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Aslam
Zubair
Z
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Lee
Myungjin
M
NeoPharm Co., Ltd, BVC-307, KRIBB, 52 Oeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
Ten
Leonid N
LN
National University of Uzbekistan, Students town, Tashkent 700-174, Uzbekistan.|Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Yang
Deok-Chun
DC
Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 449-701, Republic of Korea.
Lee
Sung-Taik
ST
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S