title: Tumor antigen occurs in N-glycan of royal jelly glycoproteins: honeybee cells synthesize T-antigen unit in N-glycan moiety.
abstract: In our previous paper (Kimura, Y., et al., Biosci. Biotechnol. Biochem., 67, 1852-1856, 2003), we found that a complex type N-glycans containing beta1-3 galactose residue occurs on royal jelly glycoproteins. During structural analysis of minor components of royal jelly N-glycans, we found complex type N-glycans bearing both galactose and N-acetylgalactosamine residues. Detailed structural analysis of pyridylaminated oligosaccharide revealed that the newly found N-glycan had a complex type structure harboring a tumor marker (T-antigen) unit: Galbeta1-3GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-6 (Galbeta1-3GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-3) Manbeta1-4GlcNAcbeta1-4GlcNAc. To our knowledge, this may be the first report of the presence of the T-antigen unit in the N-glycan moiety of eucaryotic glycoproteins.
authors.affiliation: Department of Biofunctional Chemistry, Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Japan. yosh8mar@cc.okayama-u.ac.jp
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kimura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ushijima
authors.forename: Megumi
authors.initials: M
authors.lastname: Maeda
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Hama
authors.forename: Mariko
authors.initials: M
authors.lastname: Kimura
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okihara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugimoto
authors.forename: Hideo
authors.initials: H
authors.lastname: Yamada
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000302:isolation & purification*; D000957:Antigens, Viral, Tumor / Q000096:biosynthesis*; D001516:Bees / Q000737:chemistry / Q000276:immunology*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000737:chemistry; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000096:biosynthesis; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D021241:Spectrometry, Mass, Electrospray Ionization; D000099219:Royal Jelly
chemical_list: D000951:Antigens, Neoplasm; D000957:Antigens, Viral, Tumor; D005227:Fatty Acids; D006023:Glycoproteins; D010446:Peptide Fragments; D011134:Polysaccharides; D000099219:Royal Jelly