title: Rapid screening of topoisomerase gene mutations by a novel melting curve analysis method for early warning of fluoroquinolone-resistant Streptococcus pneumoniae emergence.
abstract: We developed a real-time PCR assay combined with melting curve analysis for rapidly genotyping quinolone resistance-determining regions (QRDR) of topoisomerase genes in Streptococcus pneumoniae. This assay was not only accurate for the screening of fluoroquinolone (FQ) resistance but also relevant as an early warning system for detecting preexisting single QRDR mutations.
authors.affiliation: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki City 852-8501, Japan.
authors.forename: Kazuko Y
authors.initials: KY
authors.lastname: Fukushima
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Hirakata
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Sugahara
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Akira
authors.initials: A
authors.lastname: Kondo
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
authors.forename: Shimeru
authors.initials: S
authors.lastname: Kamihira
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D026942:DNA Topoisomerases / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D024841:Fluoroquinolones / Q000494:pharmacology*; D005838:Genotype; D006801:Humans; D009154:Mutation; D011008:Pneumococcal Infections / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D044366:Transition Temperature
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D026942:DNA Topoisomerases