Evaluation of a multiplex PCR-based reverse line blot-hybridization assay for identification of serotype and surface protein antigens of Streptococcus agalactiae.
A 33-primer multiplex PCR-based reverse line blot (mPCR/RLB) assay was developed to identify Streptococcus agalactiae serotypes and surface protein antigen genes simultaneously. It was evaluated by using 551 clinical isolates. The mPCR/RLB assay was more sensitive than conventional serotyping, especially for protein antigen typing, but otherwise the results correlated well.
Zeng
Xianyu
X
Centre for Infectious Diseases and Microbiology (CIDM), Institute of Clinical Pathology and Medical Research (ICPMR), Westmead Hospital, Darcy Road, Westmead, New South Wales 2145, Australia.
Kong
Fanrong
F
Morgan
Julie
J
Gilbert
Gwendolyn L
GL
D000942:Antigens, Bacterial / Q000145:classification* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000379:methods*; D008565:Membrane Proteins / Q000145:classification* / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013292:Streptococcus agalactiae / Q000145:classification*
D000942:Antigens, Bacterial; D008565:Membrane Proteins