Modulation of cell adhesion and motility in the immune system by Myo1f.
Although class I myosins are known to play a wide range of roles, the physiological function of long-tailed class I myosins in vertebrates remains elusive. We demonstrated that one of these proteins, Myo1f, is expressed predominantly in the mammalian immune system. Cells from Myo1f-deficient mice exhibited abnormally increased adhesion and reduced motility, resulting from augmented exocytosis of beta2 integrin-containing granules. Also, the cortical actin that co-localizes with Myo1f was reduced in Myo1f-deficient cells. In vivo, Myo1f-deficient mice showed increased susceptibility to infection by Listeria monocytogenes and an impaired neutrophil response. Thus, Myo1f directs immune cell motility and innate host defense against infection.
Kim
Sangwon V
SV
Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA.
Mehal
Wajahat Z
WZ
Dong
Xuemei
X
Heinrich
Volkmar
V
Pypaert
Marc
M
Mellman
Ira
I
Dembo
Micah
M
Mooseker
Mark S
MS
Wu
Dianqing
D
Flavell
Richard A
RA
D000199:Actins / Q000378:metabolism; D000818:Animals; D018821:CD18 Antigens / Q000378:metabolism; D002448:Cell Adhesion; D015550:Cell Degranulation; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D015169:Colony Count, Microbial; D003594:Cytoplasmic Granules / Q000378:metabolism; D005089:Exocytosis; D007113:Immunity, Innate; D008024:Ligands; D008089:Listeria monocytogenes / Q000254:growth & development; D008088:Listeriosis / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D018345:Mice, Knockout; D024461:Myosin Type I / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D009240:N-Formylmethionine Leucyl-Phenylalanine; D018375:Neutrophil Activation; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*
D000199:Actins; D018821:CD18 Antigens; D008024:Ligands; C514128:Myo1f protein, mouse; D009240:N-Formylmethionine Leucyl-Phenylalanine; D024461:Myosin Type I