The effect of laser on remanescent liver tissue after 90% hepatectomy in rats.
OBJECTIVE
To evaluate the effect of laser beam on reminiscent liver after partial hepatectomy 90%.


METHODS
Wistar rats, (N=42), were divided into six groups with seven specimens each. The partial hepatectomy (HP) was performed in all animals through exeresis of approximately 90% of the liver parenchyma. The animals from groups HP and Laser application, HPL24, HPL48 and HPL72 undertook laser irradiation carried out through application (dose of 22.5 J/cm2) in five different sites in the reminiscent liver. The rats were then sacrificed 24, 48 and 72 hours after HP procedure, for the liver regeneration analysis,using the Proliferating Cell Nuclear Antigen (PCNA),and for dosage of serum aminotransferases.


RESULTS
Were demonstrated an increase of the serum levels of alanine aminotransferase for the group of 24 hours and a decrease for the group of 72 hours exposed to laser. The index of marked cells had a considerable more improvement for the group of 72 hours exposed in laser compared to other groups.


CONCLUSIONS
Laser did not cause hepatic injuries additional to the partial hepatectomy and perhaps led to a benefit by stimulating the proliferative activity.
Oliveira
Alexandre Ferreira
AF
Departament of Surgery and Anatomy, FMRP, USP, Brazil.
Silva
Tiago Castro e
TC
Sankarankutty
Ajith Kumar
AK
Pacheco
Eduardo Garcia
EG
Ferreira
Juliana
J
Bagnato
Vanderlei Salvador
VS
Zucoloto
Sergio
S
Silva
Orlando de Castro e
Ode C
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004195:Disease Models, Animal; D006498:Hepatectomy; D053685:Laser Therapy; D008099:Liver / Q000528:radiation effects*; D017114:Liver Failure, Acute / Q000532:radiotherapy / Q000601:surgery; D008115:Liver Regeneration / Q000528:radiation effects*; D028022:Low-Level Light Therapy / Q000592:standards*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D000637:Transaminases / Q000097:blood
D015415:Biomarkers; D018809:Proliferating Cell Nuclear Antigen; D000637:Transaminases