title: Detection of human papillomavirus in cervical cell specimens by hybrid capture and PCR with different primers.
abstract: The purpose of this study was to compare hybrid capture assay with PCRs using different primers for the L1, E6-E7 regions for the detection of human papillomavirus (HPV) genome. One hundred twenty-five cervical smears with normal (n=42) and abnormal (n=83) cytology were investigated. Those at high-risk for HPV were studied by hybridization antibody capture assay and PCR with the pU-1M/pU-2R primers. Target DNA from the HPV L1 region was amplified by SPF10 primer set and home-PCR with MY09/MY11 primers. The presence of HPV DNA in cervical smears was detected by SPF10 (in 72% of cases), MY09/MY11 (58%), hybrid capture (55%) and pU-1M/pU-2R (39%). Results obtained with the SPF10 and MY09/MY11 consensus primer sets as well as hybrid capture and pU-1M/pU-2R specific for high-risk types differed significantly (chi2, P<0.0005). The correlation between assays with the use of SPF10 and MY09/MY11 was 86% and between hybrid capture and the pU-1M/pU2R technique--78%. In 49% of samples HPV DNA was detected by the four methods, whereas in 12% only by the SPF10 primers. The most sensitive technique was found to be PCR with the use of SPF10 primers, while the most specific--the MY09/11 PCR method. It seems that home-PCR with MY09/MY11 primers could be applied in screening tests.
authors.affiliation: Jagiellonian University Medical College, Chair of Microbiology, Department of Virology, Kraków, Poland. sszostek@cm-uj.krakow.pl
authors.forename: Slawa
authors.initials: S
authors.lastname: Szostek
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Klimek
authors.forename: Barbara
authors.initials: B
authors.lastname: Zawilinska
authors.forename: Janusz
authors.initials: J
authors.lastname: Rys
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kope
authors.forename: Ewa
authors.initials: E
authors.lastname: Daszkiewic
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear; D002584:Cervix Uteri / Q000166:cytology / Q000821:virology*; D017931:DNA Primers; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D015347:RNA Probes; D012680:Sensitivity and Specificity; D014626:Vaginal Smears
chemical_list: D000974:Antibodies, Antinuclear; D017931:DNA Primers; D015344:DNA Probes, HPV; D004279:DNA, Viral; D015347:RNA Probes