title: Atg26 is not involved in autophagy-related pathways in Saccharomyces cerevisiae.
abstract: Autophagy is a degradative pathway conserved among eukaryotes. It is a major route for degradation of long-lived proteins and entire organelles, such as peroxisomes. Atg26, a sterol glucosyltransferase, is specifically required for micro- and macropexophagy, but not for starvation-induced bulk autophagy in Pichia pastoris. Here we study the requirement of Saccharomyces cerevisiae Atg26 in the Cvt pathway, nonspecific autophagy and pexophagy. Our results show that the S. cerevisiae atg26Delta strain is not defective in prApe1 maturation, macroautophagy or peroxisome degradation, in contrast to the situation seen in Pichia pastoris. These studies highlight the importance of examining mutants in multiple organisms.
authors.affiliation: Life Sciences Institute, Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Klionsky
mesh_terms: D001343:Autophagy / Q000235:genetics / Q000502:physiology*; D000071183:Autophagy-Related Proteins; D005964:Glucosyltransferases / Q000235:genetics / Q000502:physiology*; D053858:Metabolic Networks and Pathways / Q000502:physiology*; D030781:Organisms, Genetically Modified; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D014617:Vacuoles / Q000378:metabolism
chemical_list: D000071183:Autophagy-Related Proteins; D029701:Saccharomyces cerevisiae Proteins; C518958:Atg26 protein, S cerevisiae; D005964:Glucosyltransferases