Elevated serum 8-oxo-dG in hemodialysis patients: a marker of systemic inflammation?
Does inflammation, as assessed by high sensitivity C-reactive protein (hs-CRP), in patients with end-stage renal disease (ESRD) tightly associate with increased serum levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8- oxo-dG)? Increased oxidative stress and inflammation have both been highlighted among several nontraditional risk factors for cardiovascular disease, which is the main cause of mortality in ESRD patients. In contrast to oxidative stress effects on proteins and lipids, DNA base damage has not been well demonstrated in ESRD. Two groups of hemodialysis patients were studied, one group with persistent inflammation (n = 13, with constant elevation of CRP > 10 mg/L for 6 months) and one group of noninflamed patients (n = 19, with constant CRP < 10 mg/L for 6 months). Serum 8-oxo-dG was significantly elevated in persistent inflammation in comparison to noninflamed patients. At an individual level, a significant correlation was found between serum 8-oxo-dG and hsCRP. Extracellular 8-oxo-dG leads to intracellular oxidative damage on the nucleotide pool, thus providing a sensitive marker for inflammatory response. Serum levels of 8-oxo-dG, in combination with other inflammatory markers, serve as useful diagnostic tools for identification of patients in risk for inflammatory complications.
Haghdoost
Siamak
S
Department of Genetics, Microbiology and Toxicology, Stockholm University, Stockholm, Sweden.
Maruyama
Yukio
Y
Pecoits-Filho
Roberto
R
Heimburger
Olof
O
Seeberger
Astrid
A
Anderstam
Björn
B
Suliman
Mohamed E
ME
Czene
Stefan
S
Lindholm
Bengt
B
Stenvinkel
Peter
P
Harms-Ringdahl
Mats
M
D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000032:analysis; D004247:DNA / Q000276:immunology; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000150:complications / Q000175:diagnosis*; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000150:complications / Q000276:immunology; D009711:Nucleotides / Q000276:immunology; D018384:Oxidative Stress / Q000276:immunology; D011237:Predictive Value of Tests; D006435:Renal Dialysis
D015415:Biomarkers; D009711:Nucleotides; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002097:C-Reactive Protein; D004247:DNA; D003849:Deoxyguanosine