Determination of protein association constants by electrophoresis.
An electrophoresis cell with scanning UV-absorption optics is presented. It allows the measurement of moving reaction boundaries of dilute protein solutions with a high-resolution. The protein profiles in the boundaries can be extrapolated to infinite time after an appropriate transformation of space and time coordinates and then evaluated with respect to association constants. This is demonstrated for the dimer-tetramer equilibrium of haemoglobin.
Matejec
R
R
Institut für Physikalische Chemie, Rheinisch - Westfälische Technische Hochschule Aachen, D-52062 Aachen, Germany.
Schönert
H
H