Effect of sudden salinity change on Penaeus latisulcatus Kishinouye osmoregulation, ionoregulation and condition in inland saline water and potassium-fortified inland saline water.
Two trials were conducted to determine the effect of sudden decrease in salinity of raw and potassium-fortified inland saline water on western king prawn Penaeus latisulcatus osmoregulation, ionoregulation and condition. Prawns were subjected to salinity decrease over 1 h from 32 to 25 ppt in the first trial and from 27 to 20 ppt in the second trial in three water types: inland saline water with potassium fortified to 100% and 80% of the marine water concentration (IS100, IS80), and raw inland saline water (ISW). In the first trial condition and ingestion rate were monitored over 19 days following salinity change. In the second trial condition, haemolymph osmo- and iono-regulation were recorded over 48 h following salinity change. In the first trial, 100% mortality was observed in ISW by day 13, with final survival 94% in IS80 and 100% in IS100. Tail muscle moisture content increased significantly (P < 0.05) over time in both trials and in all water types, suggesting loss of energy reserves. In the second trial, serum osmolality, sodium concentration and osmoregulatory capacity decreased following salinity change, stabilising by 24 h in IS100 and IS80 but continuing to decrease till 48 h in ISW, suggesting partial breakdown of osmoregulatory function in the potassium-deficient medium. Prawns were stronger regulators of divalent than monovalent cations. These trials demonstrate that potassium-deficient inland saline water requires fortification with potassium to allow prawn survival and efficient osmoregulation.
Prangnell
David I
DI
Department of Applied Biosciences, Muresk Institute, Curtin University of Technology, Perth, Western Australia, Australia. dprangnell@hotmail.com
Fotedar
Ravi
R
D000818:Animals; D002118:Calcium / Q000097:blood; D005618:Fresh Water; D006458:Hemolymph / Q000502:physiology; D007477:Ions / Q000378:metabolism*; D008274:Magnesium / Q000097:blood; D009994:Osmolar Concentration; D033561:Penaeidae / Q000502:physiology*; D011188:Potassium / Q000008:administration & dosage / Q000097:blood; D012623:Seawater; D012965:Sodium Chloride / Q000008:administration & dosage*; D013455:Sulfur / Q000097:blood; D014882:Water-Electrolyte Balance / Q000187:drug effects*
D007477:Ions; D012965:Sodium Chloride; D013455:Sulfur; D008274:Magnesium; D011188:Potassium; D002118:Calcium