title: Liquid chromatographic/tandem mass spectrometric method for the quantitation of tranilast in human plasma.
abstract: An analytical method for the determination of tranilast in human plasma using tramadol as the internal standard has been developed based on liquid chromatography/tandem mass spectrometry. Sample preparation involved protein precipitation with methanol. Separation by reversed-phase high-performance liquid chromatography using methanol/10 mM ammonium acetate (70: 30, v/v) as mobile phase was complete in a run time of 2.4 min. Detection on a Q TRAP system used multiple reaction monitoring. The method was linear in the range 0.06-20 microg/mL with intra- and inter-day precisions (as relative standard deviation) of 2.2-2.6% and 2.3-2.9%, respectively. Accuracy (as relative error) was <-2.5%. The method was applied in a pharmacokinetic study in healthy volunteers treated with a single 80 mg oral dose of tranilast.
authors.affiliation: Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130021, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lan
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.forename: Yingwu
authors.initials: Y
authors.lastname: Wang
authors.forename: J Paul
authors.initials: JP
authors.lastname: Fawcett
authors.forename: Jingkai
authors.initials: J
authors.lastname: Gu
mesh_terms: D000284:Administration, Oral; D000328:Adult; D018926:Anti-Allergic Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D062367:ortho-Aminobenzoates / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D018926:Anti-Allergic Agents; D062367:ortho-Aminobenzoates; C012293:tranilast