The translocated Salmonella effector proteins SseF and SseG interact and are required to establish an intracellular replication niche.
The facultative intracellular pathogen Salmonella enterica causes a variety of diseases, including gastroenteritis and typhoid fever. Inside epithelial cells, Salmonella replicates in vacuoles, which localize in the perinuclear area in close proximity to the Golgi apparatus. Among the effector proteins translocated by the Salmonella pathogenicity island 2-encoded type III secretion system, SifA and SseG have been shown necessary but not sufficient to ensure the intracellular positioning of Salmonella vacuoles. Hence, we have investigated the involvement of other secreted effector proteins in this process. Here we show that SseF interacts functionally and physically with SseG but not SifA and is also required for the perinuclear localization of Salmonella vacuoles. The observations show that the intracellular positioning of Salmonella vacuoles is a complex phenomenon resulting from the combined action of several effector proteins.
Deiwick
Jörg
J
Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany. deiwick@biochem.uni-luebeck.de
Salcedo
Suzana P
SP
Boucrot
Emmanuel
E
Gilliland
Sarah M
SM
Henry
Thomas
T
Petermann
Nele
N
Waterman
Scott R
SR
Gorvel
Jean-Pierre
JP
Holden
David W
DW
Méresse
Stéphane
S
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D019779:Salmonella enterica / Q000235:genetics / Q000502:physiology*; D014617:Vacuoles / Q000737:chemistry / Q000382:microbiology* / Q000648:ultrastructure
D001426:Bacterial Proteins