title: Analysis of type I and IV collagens by FT-IR spectroscopy and imaging for a molecular investigation of skeletal muscle connective tissue.
abstract: Many muscular diseases result from abnormal organization of connective tissue and/or collagen network formation. Only a few molecular imaging techniques are able to analyze this collagen network by differentiating collagen types. In this study, FT-IR spectroscopy was used to analyze type I and IV collagens, the most important compounds of which are perimysium and endomysium, respectively. Secondary structure of collagen types was determined by curve-fitting the 1,700-1,480 cm(-1) spectral interval. Type I collagen could be differentiated from type IV by its higher amounts of triple helix and alpha-helix, but lower amounts of beta-sheets (P < 0.01). FT-IR imaging was then used to determine structural features of perimysium and endomysium collagen network in bovine Flexor carpi radialis muscle. Secondary structure of proteins contained in perimysium and endomysium was found to be very close to type I and IV collagens, respectively. FT-IR spectroscopy and imaging are thus analytical tools that might be used for investigating biodistribution and assembly of collagen types in connective tissues.
authors.affiliation: CNRS UMR 5084, CNAB, Bio-Organic Chemistry Group, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France. cyril.petibois@u-bordeaux2.fr
authors.forename: Cyril
authors.initials: C
authors.lastname: Petibois
authors.forename: Gilles
authors.initials: G
authors.lastname: Gouspillou
authors.forename: Katia
authors.initials: K
authors.lastname: Wehbe
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Delage
authors.forename: Gérard
authors.initials: G
authors.lastname: Déléris
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D002417:Cattle; D024042:Collagen Type I / Q000032:analysis* / Q000737:chemistry; D024141:Collagen Type IV / Q000032:analysis* / Q000737:chemistry; D003238:Connective Tissue / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry*; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D024042:Collagen Type I; D024141:Collagen Type IV