[Progress in research of chitosan as a non-viral gene delivery vector].
Efficiency of non-viral gene delivery based on chitosan and chitosan derivatives as DNA condensing carrier is dependent on a series of factors, such as complex size, the charge ratio of chitosan/DNA, molecular mass of chitosan, the degree of chitosan deacetylation, pH and serum concentration of the transfection medium. Through modifying the chitosan in a certain extent, we can change the efficiency of transfection. Studies on transfection condition, efficiency and mechanism using chitosan and chitosan derivatives as transfection agents are reviewed.
Su
Hui-Shuang
HS
Biomedicine R&D Center, Jinan University, Guangzhou 510632, China. shsh589@163.com
Wang
Yi-Fei
YF
D000818:Animals; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D004247:DNA; D048271:Chitosan