title: Profile of histone lysine methylation across transcribed mammalian chromatin.
abstract: Complex patterns of histone lysine methylation encode distinct functions within chromatin. We previously reported that trimethylation of lysine 9 of histone H3 (H3K9) occurs at both silent heterochromatin and at the transcribed regions of active mammalian genes, suggesting that the extent of histone lysine methylation involved in mammalian gene activation is not completely defined. To identify additional sites of histone methylation that respond to mammalian gene activity, we describe here a comparative assessment of all six known positions of histone lysine methylation and relate them to gene transcription. Using several model loci, we observed high trimethylation of H3K4, H3K9, H3K36, and H3K79 in the transcribed region, consistent with previous findings. We identify H4K20 monomethylation, a modification previously linked with repression, as a mark of transcription elongation in mammalian cells. In contrast, H3K27 monomethylation, a modification enriched at pericentromeric heterochromatin, was observed broadly distributed throughout all euchromatic sites analyzed, with selective depletion in the vicinity of the transcription start sites at active genes. Together, these results underscore that similar to other described methyl-lysine modifications, H4K20 and H3K27 monomethylation are versatile and dynamic with respect to gene activity, suggesting the existence of novel site-specific methyltransferases and demethylases coupled to the transcription cycle.
authors.affiliation: The Children's Hospital of Philadelphia, Division of Hematology, Philadelphia, PA 19104, USA.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Vakoc
authors.forename: Mira M
authors.initials: MM
authors.lastname: Sachdeva
authors.forename: Hongxin
authors.initials: H
authors.lastname: Wang
authors.forename: Gerd A
authors.initials: GA
authors.lastname: Blobel
mesh_terms: D002843:Chromatin / Q000235:genetics* / Q000378:metabolism*; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000378:metabolism*; D008745:Methylation; D008780:Methyltransferases / Q000502:physiology; D039122:Poly(A)-Binding Protein I / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D006657:Histones; D039122:Poly(A)-Binding Protein I; D008780:Methyltransferases; D008239:Lysine