title: Signature-tagged transposon mutagenesis identifies novel Mycobacterium tuberculosis genes involved in the parasitism of human macrophages.
abstract: Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.
authors.affiliation: Unit of Mycobacterial Genetics, Institut Pasteur, Paris, France.
authors.forename: Vania
authors.initials: V
authors.lastname: Rosas-Magallanes
authors.forename: Gustavo
authors.initials: G
authors.lastname: Stadthagen-Gomez
authors.forename: Jean
authors.initials: J
authors.lastname: Rauzier
authors.forename: Luis B
authors.initials: LB
authors.lastname: Barreiro
authors.forename: Ludovic
authors.initials: L
authors.lastname: Tailleux
authors.forename: Frédéric
authors.initials: F
authors.lastname: Boudou
authors.forename: Ruth
authors.initials: R
authors.lastname: Griffin
authors.forename: Jérome
authors.initials: J
authors.lastname: Nigou
authors.forename: Mary
authors.initials: M
authors.lastname: Jackson
authors.forename: Brigitte
authors.initials: B
authors.lastname: Gicquel
authors.forename: Olivier
authors.initials: O
authors.lastname: Neyrolles
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics*; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D008264:Macrophages / Q000469:parasitology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016296:Mutagenesis; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000472:pathogenicity*
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements