Tyramine in the assessment of regional adrenergic function.
Regional adrenergic function is difficult to assess in humans. Tyramine given through a microdialysis probe may be a useful tool in this regard. However, tyramine data is hard to interpret given the drug's complex mode of action. We characterized the response to tyramine, isoproterenol, and dopamine in adipose tissue with microdialysis probes in normal subjects. We measured glycerol concentrations to follow changes in lipolysis and monitored tissue perfusion with ethanol dilution. During perfusion with tyramine, dialysate glycerol concentration increased dose-dependently from 83+/-8 microM at baseline to 181+/-18 microM at 3.5 mM tyramine (p<0.001) followed by a fall down to 121+/-9 microM at 35 mM tyramine (p<0.001). Propranolol almost completely blocked this response. A similar lipolytic response was not observed in isolated human adipocytes. Dopamine <35 microM did not replicate the tyramine-induced lipolysis; however, dopamine >35 microM potently inhibited lipolysis. We conclude that tyramine-induced lipolysis is explained by a pre-synaptic mechanism. Tyramine applied through a microdialysis probe in concentrations up to 3.5 mM can be used to assess pre- and post-synaptic mechanisms regulating lipid mobilization.
Adams
F
F
Universitary Medicine, Charité Campus Buch, Franz-Volhard Clinical Research Center and HELIOS Klinikum, Berlin, Germany. Frauke.Adams@charite.de
Boschmann
M
M
Schaller
K
K
Franke
G
G
Gorzelniak
K
K
Janke
J
J
Klaus
S
S
Luft
F C
FC
Heer
M
M
Jordan
J
J
D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D018759:Adrenergic Uptake Inhibitors / Q000494:pharmacology*; D000328:Adult; D004298:Dopamine / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005990:Glycerol / Q000378:metabolism*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects; D008297:Male; D017551:Microdialysis; D008875:Middle Aged; D010477:Perfusion; D012885:Skinfold Thickness; D050151:Subcutaneous Fat / Q000187:drug effects / Q000378:metabolism; D014439:Tyramine / Q000494:pharmacology*
D018759:Adrenergic Uptake Inhibitors; D007545:Isoproterenol; D005990:Glycerol; D004298:Dopamine; D014439:Tyramine