[Application of p-aminothiophenol as a SERS reporter in immunoassay].
The adsorption of p-aminothiophenol (PATP) molecule on the gold electrode was investigated. The result suggests that PATP was adsorbed perpendicularly onto the substrate. By immobilizing goat anti-mouse IgG on a glass slide modified by special organic molecules, the antibody/antigen/reporter-labeled immunogold nanoparticle sandwich assay was build for surface-enhanced Raman spectroscopy (SERS) measurements. The p-aminothiophenol (PATP) was used as SERS-reporter molecule. Thus the authors can identify the single type biospecific species by SERS signal of the reporter molecules which are coimmobilized with antibody molecules in gold colloids.
Jiang
Yun
Y
Department of Chemistry and Chemical Engineering, Suzhou University, Suzhou 215006, China.
Cui
Yan
Y
Gu
Ren-Ao
RA
D000906:Antibodies / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000032:analysis*
D000906:Antibodies; D010636:Phenols; D013438:Sulfhydryl Compounds; D006046:Gold; C042983:thiophenol