High-performance liquid chromatographic enantioseparations on capillary columns containing crosslinked polysaccharide phenylcarbamate derivatives attached to monolithic silica.
Monolithic capillary columns containing native silica gel were covalently modified with 3,5-disubstituted phenylcarbamate derivatives of cellulose and amylose and applied for enantioseparations in capillary LC. The method previously used for covalent immobilization of polysaccharide phenylcarbamate derivatives onto the surface of microparticulate silica gel was successfully adapted for in situ modification of monolithic fused-silica capillary columns. The effects of the nature of polysaccharide and the substituents, as well as of multiple covalent immobilization of polysaccharide derivative on chromatographic performance of capillary columns were studied. The capillary columns obtained using this technique are stable in all solvents commonly used in LC and exhibit promising enantiomer resolving ability.
Chankvetadze
Bezhan
B
Molecular Recognition and Separation Science Laboratory, School of Chemistry, Tbilisi State University, Tbilisi, Georgia, USA.
Kubota
Takateru
T
Ikai
Tomoyuki
T
Yamamoto
Chiyo
C
Kamigaito
Masami
M
Tanaka
Nobuo
N
Nakanishi
Kazuki
K
Okamoto
Yoshio
Y
D000688:Amylose / Q000031:analogs & derivatives / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003432:Cross-Linking Reagents; D005782:Gels; D015394:Molecular Structure; D048448:Phenylcarbamates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism
D003432:Cross-Linking Reagents; D005782:Gels; D048448:Phenylcarbamates; D011134:Polysaccharides; D058428:Silica Gel; D012822:Silicon Dioxide; D002482:Cellulose; D000688:Amylose