N-linked neutral oligosaccharides in the stratum corneum of normal and ichthyotic skin.
N-Glycan oligosaccharides are thought to play multiple, important roles in a variety of biological events. However, N-glycan profiles in the stratum corneum of human skin have not yet been studied in detail. To clarify the N-glycan profiles in the stratum corneum of normal and ichthyotic epidermis, N-glycan profiles were studied by high-performance liquid chromatography using normal human epidermal samples and scales from hyperkeratotic skin of ichthyosis patients. Chromatograms of patient scale samples showed unique alterations in three peaks eluted at 15.8, 18.8 and 26.9 min. The N-glycan profiles were significantly altered in ichthyotic hyperkeratotic skin compared with normal non-hyperkeratotic controls. These findings indicate the reduction of N-acetylglucosaminyltransferase II and fucosyltransferase 8 activities. Alteration of N-glycan structures in hyperkeratotic skin suggests the biological role of N-glycans in keratinization.
Ito
Hiroko
H
Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, 060-8638, Japan.
Akiyama
Masashi
M
Nakagawa
Hiroaki
H
Uematsu
Rie
R
Deguchi
Kisaburo
K
McMillan
James R
JR
Nishimura
Shin-Ichiro
S
Shimizu
Hiroshi
H
D002851:Chromatography, High Pressure Liquid; D004817:Epidermis / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D007057:Ichthyosis / Q000473:pathology*; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry
D009844:Oligosaccharides; D011134:Polysaccharides