title: Expression of basic fibroblast growth factor results in the decrease of myostatin mRNA in murine C2C12 myoblasts.
abstract: During the development and regeneration of skeletal muscle, many growth factors, such as basic fibroblast growth factor (bFGF, FGF-2) and myostatin, have been shown to play regulating roles. bFGF contributes to promote proliferation and to inhibit differentiation of skeletal muscle, whereas myostatin plays a series of contrasting roles. In order to elucidate whether the expression of bFGF has any relationship with the expression of myostatin in skeletal muscle cells, we constructed a eukaryotic expression vector for the expression of exogenous bFGF in murine C2C12 myoblasts. Quantitative RT-PCR assays indicated that with the increase of the expression of exogenous bFGF gene, the expression of endogenous myostatin gene was suppressed at mRNA level and protein level.
authors.affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100094, China.
authors.forename: Hua-Zhong
authors.initials: HZ
authors.lastname: Liu
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Xing-Yuan
authors.initials: XY
authors.lastname: Yang
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: An
authors.forename: Yong-Fu
authors.initials: YF
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D015536:Down-Regulation / Q000502:physiology*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D051379:Mice; D032446:Myoblasts / Q000378:metabolism*; D055435:Myostatin; D012333:RNA, Messenger / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C525911:MSTN protein, human; C525912:Mstn protein, mouse; D055435:Myostatin; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2