title: Oriented loading of FtsK on KOPS.
abstract: In Escherichia coli, the ATP-dependent DNA translocase FtsK transports DNA across the site of cell division and activates recombination by the XerCD recombinases at a specific site on the chromosome, dif, to ensure the last stages of chromosome segregation. DNA transport by FtsK is oriented by 8-base-pair asymmetric sequences ('KOPS'). Here we provide evidence that KOPS promote FtsK loading on DNA and that translocation is oriented at this step.
authors.affiliation: Laboratoire de Microbiologie et de Génétique Moléculaire, CNRS UMR5100, Toulouse, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Bigot
authors.forename: Omar A
authors.initials: OA
authors.lastname: Saleh
authors.forename: François
authors.initials: F
authors.lastname: Cornet
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Allemand
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Barre
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism*; D019426:Integrases / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D017434:Protein Structure, Tertiary
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C096452:FtsK protein, E coli; D008565:Membrane Proteins; C473360:XerC protein, E coli; D000255:Adenosine Triphosphate; D019426:Integrases; C473361:XerD protein, E coli