[Production of gastrodin through biotransformation of p-hydroxybenzaldehyde by cell suspension cultures of Datura tatula L].
The conversion of exogenous p-hydroxybenzaldehyde to p-hydroxy-methyl-phenol-beta-D-glucoside (gastrodin) was studied by using cell suspension culture of Datura tatula L. The chemical structure of this synthesized gastrodin was identified based on the spectral analysis and chemical evidence. The conversion procedure of p-hydroxybenzaldehyde into gastrodin by D. tatula L. cell suspension cultures was established. The synthesized gastrodin (II) was isolated from the ferment liquor and identified by spectral analysis. At the same time, the p-hydroxybenzyl alcohol (I) converted through biotransformation of p-hydroxybenzaldehyde by cell suspension cultures of D. tatula L. was also isolated and identified. The efficiency of glucosylation of p-hydroxybenzaldehyde was remarkably enhanced by adding salicylic acid (0.1 mg/L) and keeping the lower pressure (0.001MPa) in 25L airlift loop bioreactor. The biotransformation of exogenous p-hydroxybenzaldehyde to gastrodin by cell suspension culture of D. tatula L. is a promising approach.
Gong
Jia-Shun
JS
Faculty of Food Science and Technology, Yunnan Agricultural University, Kunming, China. gong199@163.com
Ma
Wei-Peng
WP
Pu
Jun-Xue
JX
Xu
Shu-Guan
SG
Zheng
Shuang-Qing
SQ
Xiao
Chun-Jie
CJ
D001547:Benzaldehydes / Q000378:metabolism*; D001592:Benzyl Alcohols / Q000737:chemistry; D019149:Bioreactors; D001711:Biotransformation; D032302:Datura / Q000378:metabolism*; D005960:Glucosides / Q000096:biosynthesis* / Q000737:chemistry; D020156:Salicylic Acid / Q000494:pharmacology; D013535:Suspensions
D001547:Benzaldehydes; D001592:Benzyl Alcohols; D005960:Glucosides; D013535:Suspensions; C045345:gastrodin; C011483:4-hydroxybenzaldehyde; D020156:Salicylic Acid