title: [Genetic environment of CTX-M-2 in Klebsiella pneumoniae isolates from hospitalized patients in Uruguay].
abstract: We studied two CTX-M-2-producing Klebsiella pneumoniae clinical strains, K96005 and K13, isolated from hospitalized patients in Uruguay, during 1996 and 2003, respectively. The genomic surroundings of bla(CTX-M-2) were characterized by PCR-mapping and DNA sequencing. Our results show that blaCTX-M-2 is included in a complex class-1 integron (InK13), associated with an orf513 in both isolates. The genetic array of the integron, aac(6')-lb, bla(OxA,2), orfD (gene cassette region), associated with an orf513-bla(CTX-M-2), seems to be widely disseminated over the Rio de la Plata region.
authors.affiliation: Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Alfredo Navarro 3051, Montevideo, Uruguay.
authors.forename: R
authors.initials: R
authors.lastname: Vignoli
authors.forename: N
authors.initials: N
authors.lastname: Cordeiro
authors.forename: V
authors.initials: V
authors.lastname: Seija
authors.forename: F
authors.initials: F
authors.lastname: Schelotto
authors.forename: M
authors.initials: M
authors.lastname: Radice
authors.forename: J
authors.initials: J
authors.lastname: Ayala
authors.forename: P
authors.initials: P
authors.lastname: Power
authors.forename: G
authors.initials: G
authors.lastname: Gutkind
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D032023:Integrons; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014583:Uruguay; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; C071167:beta-lactamase CTX-2; C515384:beta-lactamase CTX-M-2, Klebsiella pneumoniae; D001618:beta-Lactamases