Simultaneous determination of 11 characteristic components in three species of Curcuma rhizomes using pressurized liquid extraction and high-performance liquid chromatography.
A high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) and pressurized liquid extraction was developed for simultaneous quantitative determination of 11 characteristic compounds, including curcumenone, curcumenol, neocurdione, curdione, isocurcumenol, furanodienone, curcumol, germacrone, curzerene, furanodiene and beta-elemene, in rhizomes of three species of Curcuma. The analysis was performed on an ODS C18 column. The mobile phase consisted of (A) water and (B) acetonitrile using a gradient elution. The peaks were monitored at both 214 nm and 256 nm. All calibration curves showed good linearity (r2 > 0.9996) within test ranges. This method showed good repeatability for the quantification of these eleven components in three species Curcuma rhizomes with intra- and inter-day variations of less than 1.57% and 1.98%, respectively. The validated method was successfully applied to quantify 11 investigated components in eighteen samples of three species of Curcuma, which is helpful to control their quality.
Yang
F Q
FQ
Institute of Chinese Medical Sciences, University of Macau, Macau, China.
Wang
Y T
YT
Li
S P
SP
D000097:Acetonitriles / Q000378:metabolism; D002138:Calibration; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D030024:Curcuma / Q000737:chemistry* / Q000145:classification*; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D012044:Regression Analysis; D027343:Rhizome / Q000737:chemistry*
D000097:Acetonitriles; D010936:Plant Extracts