title: Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses.
abstract: A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) method was developed for simultaneous detection and typing/subtyping of influenza viruses A/H1, A/H3 or B, and respiratory syncytial viruses A or B, followed by DNA semiquantitation using the Agilent 2100 Bioanalyzer. Such method provides a rapid, specific and sensitive diagnostic tool for detection and semiquantification of respiratory illness specimens.
authors.affiliation: Department of Clinical Immunology and Virology, Applied Immunology and Microbiology Division, Wyeth Vaccines Research, Bldg 180, 401N. Middletown Rd., Pearl River, NY 10965, USA.
authors.forename: X Sherry
authors.initials: XS
authors.lastname: Chi
authors.forename: Fenglan
authors.initials: F
authors.lastname: Li
authors.forename: John S
authors.initials: JS
authors.lastname: Tam
authors.forename: Ruth
authors.initials: R
authors.lastname: Rappaport
authors.forename: Sheau-Mei
authors.initials: SM
authors.lastname: Cheng
mesh_terms: D002675:Child, Preschool; D006801:Humans; D007223:Infant; D009980:Influenza A virus / Q000302:isolation & purification*; D009981:Influenza B virus / Q000302:isolation & purification*; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity