Sensitive, quantitative, and fast modifications for Coomassie Blue staining of polyacrylamide gels.
In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons: Low price, Visible with the eye, Desk top scanners can be employed for image acquisition, Better for quantitative analysis than silver staining, Possible modifications for fast or highly sensitive staining, Mass spectrometry compatible.
Westermeier
Reiner
R
GE Healthcare Life Sciences, Oskar-Schlemmer-Strasse 11, D-80807 Munich, Germany. reiner.westermeier@ge.com
D003102:Colloids; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012394:Rosaniline Dyes; D012680:Sensitivity and Specificity; D016622:Silver Staining / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*
D003102:Colloids; D011506:Proteins; D012394:Rosaniline Dyes; C024757:Coomassie brilliant blue R; C004692:coomassie Brilliant Blue