Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy.
We describe azimuthal light scattering spectroscopy (phi/LSS), a novel technique for assessing epithelial-cell nuclear morphology. The difference between the spectra measured at azimuthal angles phi = 0 degrees and phi = 90 degrees preferentially isolates the single backscattering contribution due to large (approximately 10 microm) structures such as epithelial cell nuclei by discriminating against scattering from smaller organelles and diffusive background. We demonstrate the feasibility of using phi/LSS for cancer detection by showing that spectra from cancerous colon tissue exhibit significantly greater azimuthal asymmetry than spectra from normal colonic tissues.
Yu
Chung-Chieh
CC
G R Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. chungyu@mit.edu
Lau
Condon
C
Tunnell
James W
JW
Hunter
Martin
M
Kalashnikov
Maxim
M
Fang-Yen
Christopher
C
Fulghum
Stephen F
SF
Badizadegan
Kamran
K
Dasari
Ramachandra R
RR
Feld
Michael S
MS
D002467:Cell Nucleus / Q000473:pathology*; D003110:Colonic Neoplasms / Q000473:pathology; D004847:Epithelial Cells / Q000473:pathology*; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology*; D008027:Light; D008863:Microspheres; D008928:Mitochondria / Q000473:pathology; D019047:Phantoms, Imaging; D012542:Scattering, Radiation