A plasmid-borne truncated luxI homolog controls quorum-sensing systems and extracellular carbohydrate production in Methylobacterium extorquens AM1.
A cryptic plasmid of Methylobacterium extorquens AM1 was found to encode tslI, a truncated luxI homolog. tslI was shown to be expressed and to control transcription of the acyl-homoserine lactone (HSL) synthase gene msaI and thus, indirectly, acyl-HSL production. In addition, tslI was found to positively regulate extracellular polysaccharide production.
Penalver
Carlos G Nieto
CG
Institute of Microbiology, Swiss Federal Institute of Technology Zurich, ETH Hönggerberg, Zurich, Switzerland.
Cantet
Franck
F
Morin
Danièle
D
Haras
Dominique
D
Vorholt
Julia A
JA
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis; D000222:Adaptation, Physiological / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002241:Carbohydrates / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial; D008025:Ligases / Q000096:biosynthesis* / Q000235:genetics; D020601:Methylobacterium extorquens / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002241:Carbohydrates; C085427:LuxI protein, Bacteria; D012329:RNA, Bacterial; D012333:RNA, Messenger; D014157:Transcription Factors; C088386:homoserine lactone; D008025:Ligases; C449816:N-acylhomoserine lactone synthase; D015107:4-Butyrolactone