title: [Antitumor effects of nobiletin on Heps and its mechanism].
abstract: OBJECTIVE
  To study the inhibitory effect and mechanism of nobiletin on Heps tumor bearing mice.
  
  
  METHODS
  Models of Heps tumor bearing mice were established. The inhibitory rates of tumor growth were calculated, the apoptosis morphology of tumor tissue was observed. The T lymphocyte transformation capacity was tested by MTT assay, the TNFalpha and IL-2 production were measured by LDH kits.
  
  
  RESULTS
  Nobiletin could significantly inhibit Heps tumor growth. The inhibitory rates were 42.14% - 65.09% (P < 0.01). The morphology of tumor tissues in nobiletin group had typical characters of necrosis and apoptosis through transmission electron microscope. Nobiletin could stimulate T lymphocyte transformation and the production of TNFalpha and IL-2.
  
  
  CONCLUSIONS
  Nobiletin has obvious antitumor effect on Heps, the main mechanism is to enhance the cellular immune function and induce apoptosis of tumor tissue.
authors.affiliation: The Medical and Pharmaceutical Academy, Yangzhou University, Yangzhou 225001, China. lixin2001@sohu.com
authors.forename: Hong-quan
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Ge
authors.forename: Mei-ying
authors.initials: MY
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002957:Citrus / Q000737:chemistry; D005260:Female; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000517:prevention & control* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010946:Plants, Medicinal / Q000737:chemistry; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D047309:Flavones; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; C008661:nobiletin