Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients.
Malassezia furfur is lypophilic yeast commonly associate with dermatological disorders. In the present work, we described the isolation of 47 M. furfur strains from three groups of patients: pityriasis versicolor (21 isolates), seborrhoeic dermatitis (15 isolates) and seborrhoeic dermatitis of the HIV positive patients (11 isolates). To investigate the identity of the strains at molecular level, DNA genomic of M. furfur strains were prepared and used to RAPD-PCR analyses. RAPD assay were carried out using two decamer primers and bands pattern generated were analyzed by an Unweighted Pair-Group Method (UPGMA). Dendrogram established a distinct differentiation between M. furfur isolates from pityriasis versicolor and seborrhoeic dermatitis patients with or without AIDS. We concluded that RAPD typing presented a high discriminatory power between strains studied in this work and can be applied in epidemiological investigation of skin disease causing by M. furfur.
Gandra
Rinaldo F
RF
Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069, Cascavel, 85814-110, PR, Brazil. rfgandra@unioeste.br
Simão
Rita C G
RC
Matsumoto
Flávia E
FE
da Silva
Bosco C M
BC
Ruiz
Luciana S
LS
da Silva
Eriques G
EG
Gambale
Walderez
W
Paula
Claudete R
CR
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D004271:DNA, Fungal / Q000235:genetics; D012628:Dermatitis, Seborrheic / Q000382:microbiology* / Q000821:virology; D005838:Genotype; D006678:HIV; D006801:Humans; D008290:Malassezia / Q000235:genetics* / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D014010:Tinea Versicolor / Q000382:microbiology* / Q000821:virology
D004271:DNA, Fungal