title: SEM analyses of bacterial contamination of e-PTFE membranes and GTR clinical results.
abstract: The aim of the present study was to determine whether bacterial contamination of expanded polytetrafluoroethylene (e-PTFE) membranes and the degree of membrane exposure could affect the clinical results of guided tissue regeneration (GTR). Seven patients (aged 42 to 58 years) presented with twenty intrabony defects that were treated by GTR + DFDBA (decalcified freeze-dried bone allograft). Gingival recession, probing depths, and clinical attachment levels were recorded before surgery and 10 months subsequently. Exposure of the membrane was recorded at the moment of removal. The membranes were analyzed in three distinct regions by scanning electron microscopy (SEM): collar, medial and apical regions. The presence of host cells adhering to the e-PTFE membrane was determined and bacterial contamination was quantified as per Simion et al. (1994). Statistical analysis did not show significant differences for bacterial contamination or for the presence of host cells on the membrane surface at different levels of exposure (up to 30%, from 31% to 60%, and from 61% to 75%). Results were considered statistically significant at p < 0.05, according to the Kruskal-Wallis rank sum test. Exposure of the membrane and bacterial contamination did not modify the levels recorded. Bacterial contamination had an adverse effect on bone defect filling.
authors.affiliation: Department of Periodontics, College of Dentistry, University of São Paulo, São Paulo, Brazil.
authors.forename: Maria Letícia
authors.initials: ML
authors.lastname: Rossa
authors.forename: Luiz A
authors.initials: LA
authors.lastname: Lima
authors.forename: Francisco Emílio
authors.initials: FE
authors.lastname: Pustiglioni
authors.forename: André Micheletti
authors.initials: AM
authors.lastname: Hespanhol
authors.forename: Simão
authors.initials: S
authors.lastname: Kon
authors.forename: José
authors.initials: J
authors.lastname: Grigolli Filho
authors.forename: Neil Ferreira
authors.initials: NF
authors.lastname: Novo
mesh_terms: D000328:Adult; D016025:Bone Transplantation / Q000379:methods; D016009:Chi-Square Distribution; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D010510:Periodontal Diseases / Q000601:surgery*; D011138:Polytetrafluoroethylene; D018709:Statistics, Nonparametric
chemical_list: D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene