title: Germ-line mutations in p27Kip1 cause a multiple endocrine neoplasia syndrome in rats and humans.
abstract: MENX is a recessive multiple endocrine neoplasia-like syndrome in the rat. The tumor spectrum in MENX overlaps those of human multiple endocrine neoplasia (MEN) types 1 and 2. We mapped the MenX locus to the distal part of rat chromosome 4, excluding the homologs of the genes responsible for the MEN syndromes (RET and MEN1) and syndromes with an endocrine tumor component (VHL and NF1). We report the fine mapping of the disease locus and the identification of a homozygous frameshift mutation in Cdkn1b, encoding the cyclin-dependent kinase inhibitor p27(Kip1). As a consequence of the mutation, MENX-affected rats show dramatic reduction in p27(Kip1) protein. We have identified a germ-line nonsense mutation in the human CDKN1B gene in a MEN1 mutation-negative patient presenting with pituitary and parathyroid tumors. Expanded pedigree analysis shows that the mutation is associated with the development of an MEN1-like phenotype in multiple generations. Our findings demonstrate that germ-line mutations in p27(Kip1) can predispose to the development of multiple endocrine tumors in both rats and humans.
authors.affiliation: Institutes of Pathology, GSF-National Research Center for Environment and Health, 85764 Neuherberg, Germany. natalia.pellegata@gsf.de
authors.forename: Natalia S
authors.initials: NS
authors.lastname: Pellegata
authors.forename: Leticia
authors.initials: L
authors.lastname: Quintanilla-Martinez
authors.forename: Heide
authors.initials: H
authors.lastname: Siggelkow
authors.forename: Elenore
authors.initials: E
authors.lastname: Samson
authors.forename: Karin
authors.initials: K
authors.lastname: Bink
authors.forename: Heinz
authors.initials: H
authors.lastname: Höfler
authors.forename: Falko
authors.initials: F
authors.lastname: Fend
authors.forename: Jochen
authors.initials: J
authors.lastname: Graw
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Atkinson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics* / Q000378:metabolism; D004252:DNA Mutational Analysis; D016368:Frameshift Mutation; D020022:Genetic Predisposition to Disease; D018095:Germ-Line Mutation; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009377:Multiple Endocrine Neoplasia / Q000235:genetics* / Q000378:metabolism; D010282:Parathyroid Neoplasms / Q000235:genetics; D010641:Phenotype; D010911:Pituitary Neoplasms / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016415:Sequence Alignment
chemical_list: C436291:CDKN1B protein, human; D047908:Intracellular Signaling Peptides and Proteins; C105373:MEN1 protein, human; D011518:Proto-Oncogene Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27