title: Feasibility of freeze-dried sera for serological and molecular biological detection of hepatitis B and C viruses.
abstract: We compared hepatitis B virus (HBV) surface antigen, anti-hepatitis C virus (HCV) antibody, and HCV RNA quantification in frozen and freeze-dried serum samples to assess the usefulness of freeze-dried sera for detection of HBV and HCV. The results indicated that freeze-dried sera as well as frozen sera can be useful for serological and molecular biological analyses of HBV and HCV.
authors.affiliation: Department of Clinical Studies, Radiation Effects Research Foundation, 5-2 Hijiyama Park, Minami-ku, Hiroshima, 732-0815, Japan. nwaka@rerf.or.jp
authors.forename: Waka
authors.initials: W
authors.lastname: Ohishi
authors.forename: Saeko
authors.initials: S
authors.lastname: Fujiwara
authors.forename: Gen
authors.initials: G
authors.lastname: Suzuki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kishi
authors.forename: Misae
authors.initials: M
authors.lastname: Sora
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Matsuura
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hakoda
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Tatsukawa
authors.forename: Michiko
authors.initials: M
authors.lastname: Yamada
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Chayama
mesh_terms: D005612:Freeze Drying; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006509:Hepatitis B / Q000175:diagnosis / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis / Q000821:virology; D018937:Hepatitis C Antibodies / Q000032:analysis; D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044967:Serum / Q000821:virology*; D013048:Specimen Handling / Q000379:methods*
chemical_list: D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies; D012367:RNA, Viral