S16, a novel S-RNase allele in the diploid species Solanum chacoense.
Wild potato species have a gametophytic self-incompatibility system controlled by a single multiallelic S locus. In the style, the S-RNase gene codes for an allele-specific ribonuclease that is involved in the rejection of pollen that carries the same S haplotype. This gene has 5 conserved regions (C1-C5) and highly variable regions outside of these areas that play a role in S-RNase allele specificity. In this work, PCR-mediated amplification of genomic DNA from 2 Solanum chacoense accessions was performed using primers designed on the basis of the C1 and C4 conserved regions. By sequencing the PCR products, a new S-RNase allele (S16) was identified in 1 plant of the QBCM argentinian accession. Comparison of the partial sequence (from C2 to C3) of S16 RNase with those of 11 S-RNase genes of other Solanaceae species showed the highest and the lowest similarity scores within the same plant species (respectively, 71% with the S11 and S13 RNase and 35% with the S2 RNase). Differences at the nucleotide level between S16 and S11 RNase alleles are discussed.
Marcellán
Olga N
ON
Facultad de Ciencias Agrarias, UNMdP-EEA Balcarce INTA CC, Balcarce, Argentina. omarcellan@balcarce.inta.gov.ar
Acevedo
Alberto
A
Camadro
Elsa L
EL
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D004171:Diploidy; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D032322:Solanum / Q000201:enzymology* / Q000235:genetics*
D018744:DNA, Plant; D012260:Ribonucleases