title: The association of increased p14ARF/p16INK4a and p15INK4a gene expression with proliferative activity and the clinical course of multiple myeloma.
abstract: p14/p16 and p15 gene expression was assessed by quantitative polymerase chain reaction in purified plasma cells (PC) from 52 patients with symptomatic multiple myeloma (MM) and seven with smoldering MM in order to clarify the impact of these genes on the proliferative activity of tumor cells and patients' outcome. p15 expression was lower in symptomatic MM than in smoldering SMM (-1.80 vs.1.51,p=0.026); similar results were observed for p14/p16. MM patients whose PC displayed high p15 and/or p14/p16 expression had a lower percentage of S-phase PC than the remaining cases (1.79%+/-1.35 vs. 3.04%+/-1.42, p=0.028), favorable prognostic factors and longer survival (100% vs. 49%at 2.5 years; p=0.007).
authors.affiliation: Hematology Department, University Hospital of Salamanca, Center for Cancer Research (CIC), Salamanca, Spain.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Sarasquete
authors.forename: Ramón
authors.initials: R
authors.lastname: García-Sanz
authors.forename: Adriana
authors.initials: A
authors.lastname: Armellini
authors.forename: Marta
authors.initials: M
authors.lastname: Fuertes
authors.forename: Patricia
authors.initials: P
authors.lastname: Martín-Jiménez
authors.forename: Magdalena
authors.initials: M
authors.lastname: Sierra
authors.forename: María
authors.initials: M
authors.lastname: Del Carmen Chillón
authors.forename: Miguel
authors.initials: M
authors.lastname: Alcoceba
authors.forename: Ana
authors.initials: A
authors.lastname: Balanzategui
authors.forename: Fernando
authors.initials: F
authors.lastname: Ortega
authors.forename: José Mariano
authors.initials: JM
authors.lastname: Hernández
authors.forename: Anna
authors.initials: A
authors.lastname: Sureda
authors.forename: Luis
authors.initials: L
authors.lastname: Palomera
authors.forename: Marcos
authors.initials: M
authors.lastname: González
authors.forename: Jesús Fernando
authors.initials: JF
authors.lastname: San Miguel
mesh_terms: D049109:Cell Proliferation; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000096:biosynthesis / Q000235:genetics*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D011379:Prognosis; D025803:Tumor Suppressor Protein p14ARF / Q000096:biosynthesis / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D025803:Tumor Suppressor Protein p14ARF