title: Genetic variation and population structure of oriental migratory locust, Locusta migratoria manilensis, in China by allozyme, SSRP-PCR, and AFLP markers.
abstract: Allozyme analysis, microsatellite primer PCR (SSRP-PCR), and amplified fragment length polymorphism (AFLP) techniques were used to assess genetic diversity and population structure of the Chinese oriental migratory locust, Locusta migratoria manilensis. A total of 299 PCR markers (67 SSRPs and 232 AFLPs) were detected in eight populations, of which 98.7% were polymorphic markers. The proportion of polymorphic loci (95.5-98.8%) by SSRP+AFLP markers indicated no significant differences between populations, and all populations exhibited a similar level of variability; results of the allozyme analysis demonstrated that 19 loci gave rise to a lower level of polymorphism (55.6-66.7%). The genetic distances between the populations were relatively low. Shannon's index and Nei's gene diversity showed low differentiation among the populations. Allozyme analysis, however, reflected greater similarity and smaller differentiation between the populations than those shown by SSRP and AFLP markers. Neighbor-joining dendrograms derived from both the allozyme and SSRP+AFLP markers showed that the genetic distances among Chinese oriental migratory locust populations were not greatly influenced by geographic distance and breeding habitats.
authors.affiliation: College of Life Science and Technology, Shanxi University, Taiyuan, Shanxi, PR China.
authors.forename: Xianyun
authors.initials: X
authors.lastname: Zheng
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhong
authors.forename: Yihao
authors.initials: Y
authors.lastname: Duan
authors.forename: Chunxuan
authors.initials: C
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Dang
authors.forename: Yaping
authors.initials: Y
authors.lastname: Guo
authors.forename: Enbo
authors.initials: E
authors.lastname: Ma
mesh_terms: D000818:Animals; D002681:China; D004798:Enzymes / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D048188:Locusta migratoria / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004798:Enzymes; D005819:Genetic Markers