Use of a centrifugation-based, point-of-care device for production of canine autologous bone marrow and platelet concentrates.
OBJECTIVE
To analyze a centrifugation-based, point-of-care device that concentrates canine platelets and bone marrow-derived cells.


METHODS
19 adult sexually intact dogs.


METHODS
Anticoagulated peripheral blood (60 mL) and 60 mL of anticoagulated bone marrow aspirate (BMA) were concentrated by centrifugation with the centrifugation-based, point-of-care device to form a platelet and a bone marrow concentrate (BMC) from 11 dogs. Blood samples were analyzed on the basis of hemograms, platelet count, and PCV. The BMA and BMC were analyzed to determine PCV, total nucleated cell count, RBC count, and differential cell counts. The BMC stromal cells were cultured in an osteoinductive medium. Eight additional dogs were used to compare the BMC yield with that in which heparin was infused into the bone marrow before aspiration.


RESULTS
The centrifugation-based, point-of-care device concentrated platelets by 6-fold over baseline (median recovery, 63.1%) with a median of 1,336 x 10(3) platelets/microL in the 7-mL concentrate. The nucleated cells in BMCs increased 7-fold (median recovery, 42.9%) with a median of 720 x 10(3) cells/microL in the 4-mL concentrate. The myeloid nucleated cells and mononuclear cells increased significantly in BMCs with a significant decrease in PCV, compared with that of BMAs. Stromal cell cultures expressed an osteoblastic phenotype in culture. Infusion of heparin into the bone marrow eliminated clot formation and created less variation in the yield (median recovery, 61.9%).


CONCLUSIONS
Bone marrow-derived cell and platelet-rich concentrates may form bone if delivered in an engineered graft, thus decreasing the need for cancellous bone grafts.
Thoesen
Michael S
MS
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Berg-Foels
Wendy S Vanden
WS
Stokol
Tracy
T
Rassnick
Kenneth M
KM
Jacobson
May S
MS
Kevy
Sherwin V
SV
Todhunter
Rory J
RJ
D000818:Animals; D001781:Blood Component Removal / Q000662:veterinary; D001792:Blood Platelets / Q000502:physiology*; D001854:Bone Marrow Cells / Q000502:physiology*; D002469:Cell Separation / Q000295:instrumentation* / Q000662:veterinary*; D002478:Cells, Cultured; D002498:Centrifugation / Q000295:instrumentation / Q000662:veterinary*; D004285:Dogs; D005260:Female; D008297:Male; D019095:Point-of-Care Systems; D017154:Stromal Cells