A high-throughput screening assay for hydroxynitrile lyase activity.
A high-throughput screening assay for hydroxynitrile lyase activity accepting a wide range of HNL-substrates is presented, which is useful either for enzyme fingerprinting or screening of huge variant libraries generated in metagenome or directed evolution approaches.
Andexer
Jennifer
J
Institute of Molecular Enzyme Technology, Heinrich-Heine University Düsseldorf, Forschungszentrum Jülich, 52426, Jülich, Germany.
Guterl
Jan-Karl
JK
Pohl
Martina
M
Eggert
Thorsten
T
D000446:Aldehyde-Lyases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002384:Catalysis; D003001:Cloning, Molecular; D019020:Directed Molecular Evolution / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D009570:Nitriles / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D013053:Spectrophotometry; D013379:Substrate Specificity
D009570:Nitriles; D010940:Plant Proteins; C430593:cyanohydrin; D000446:Aldehyde-Lyases