Royal jelly stimulates bone formation: physiologic and nutrigenomic studies with mice and cell lines.
Royal jelly (RJ) has diverse physiological and pharmacological functions. We observed its weak estrogenic activity in the previous study. RJ stimulated the proliferation of mouse osteoblast-like MC3T3-E1 cells at 0.1 mg/ml, and the effect was blocked by the specific estrogen receptor antagonist ICI 182,780. The addition of 0.1-1.0 mg/ml RJ enhanced collagen production in culture medium. Oral administration of RJ to normal female mice for 9 weeks increased the ash content of their tibiae. DNA microarray analysis revealed significant changes in gene expression related to extracellular matrix formation when the femurs of mice fed RJ were analyzed. Quantitative reverse transcriptase-PCR (RT-PCR) confirmed up-regulation of procollagen I alpha1 gene expression. These data suggest that RJ as a whole or some of its individual components stimulates production of type I collagen and other activities for bone formation through action on osteoblasts.
Narita
Yukio
Y
Nagaragawa Research Center, API Co., Ltd, Japan.
Nomura
Johji
J
Ohta
Shozo
S
Inoh
Yoshikazu
Y
Suzuki
Kazu-Michi
KM
Araki
Yoko
Y
Okada
Shinji
S
Matsumoto
Ichiro
I
Isohama
Yoichiro
Y
Abe
Keiko
K
Miyata
Takeshi
T
Mishima
Satoshi
S
D000818:Animals; D002460:Cell Line; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics; D005109:Extracellular Matrix / Q000235:genetics; D005227:Fatty Acids / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D020869:Gene Expression Profiling; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D016133:Polymerase Chain Reaction; D013977:Tibia; D015854:Up-Regulation / Q000187:drug effects; D000099219:Royal Jelly
D024042:Collagen Type I; D005227:Fatty Acids; D000099219:Royal Jelly