Characterization of monoclonal antibodies against fowl adenovirus serotype 1 (FAV1) isolated from gizzard erosion.
Six clones of monoclonal antibodies (Mabs) to fowl adenovirus (FAV) serotype 1 were produced. All Mabs reacted positively by enzyme-linked immunosorbent assay. Three Mabs recognized the putative 100-kD hexon protein and reacted to serotype 1 specifically by western blot analysis but did not react to other FAV serotypes (2, 3, 4, 5, 6, 7, and 8a). These Mabs will be useful for immunodiagnosis of FAV serotype 1 infection in chickens with gizzard erosion and in further research studies involving the genomes and proteins of FAV serotype 1.
Taharaguchi
Satoshi
S
Laboratory of Veterinary Microbiology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan.
Ito
Hiroko
H
Ohta
Hideyuki
H
Takase
Kozo
K
D000818:Animals; D000911:Antibodies, Monoclonal; D045744:Cell Line, Tumor; D002645:Chickens / Q000821:virology*; D025681:Fowl adenovirus A / Q000276:immunology* / Q000302:isolation & purification*; D005895:Gizzard, Avian / Q000821:virology*; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D011201:Poultry Diseases / Q000821:virology*
D000911:Antibodies, Monoclonal