Glucosylation of flavonols by Escherichia coli expressing glucosyltransferase from rice (Oryza sativa).
A glucosyltransferase cDNA, RF5, was cloned from Oryza sativa using an RT-PCR strategy and expressed in Escherichia coli. Several flavonoids were tested for their ability to serve as substrates for RF5. RF5 effectively glucosylated kaempferol and quercetin to produce their 3-O-glucosides. Thus, RF5 could be defined as a flavonol 3-O-glucosyltransferase. E. coli cells expressing RF5 effectively converted 100 microM of kaempferol and quercetin into their corresponding glucosides.
Kim
Ji Hye
JH
Division of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea.
Shin
Kwang Hee
KH
Ko
Jae Hyoung
JH
Ahn
Joong-Hoon
JH
D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000378:metabolism*; D044948:Flavonols / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry; D005964:Glucosyltransferases / Q000378:metabolism; D006031:Glycosylation; D044949:Kaempferols / Q000737:chemistry; D008956:Models, Chemical; D012275:Oryza / Q000201:enzymology*; D011794:Quercetin / Q000737:chemistry; D013997:Time Factors
D044948:Flavonols; D005960:Glucosides; D044949:Kaempferols; D011794:Quercetin; D005964:Glucosyltransferases; D005947:Glucose