title: Heme biosynthesis in Methanosarcina barkeri via a pathway involving two methylation reactions.
abstract: The methanogenic archaeon Methanosarcina barkeri synthesizes protoheme via precorrin-2, which is formed from uroporphyrinogen III in two consecutive methylation reactions utilizing S-adenosyl-L-methionine. The existence of this pathway, previously exclusively found in the sulfate-reducing delta-proteobacterium Desulfovibrio vulgaris, was demonstrated for M. barkeri via the incorporation of two methyl groups from methionine into protoheme.
authors.affiliation: Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, D-35043 Marburg, Germany.
authors.forename: Bärbel
authors.initials: B
authors.lastname: Buchenau
authors.forename: Jörg
authors.initials: J
authors.lastname: Kahnt
authors.forename: Ilka U
authors.initials: IU
authors.lastname: Heinemann
authors.forename: Dieter
authors.initials: D
authors.lastname: Jahn
authors.forename: Rudolf K
authors.initials: RK
authors.lastname: Thauer
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D006418:Heme / Q000096:biosynthesis*; D017021:Methanosarcina barkeri / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism; D008745:Methylation; D014577:Uroporphyrinogens / Q000378:metabolism; D014578:Uroporphyrins / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D003470:Culture Media; D014577:Uroporphyrinogens; D014578:Uroporphyrins; D006418:Heme; C063209:15,23-dihydrosirohydrochlorin; D008715:Methionine; C038344:methionine methyl ester