[Study on vitrification of porcine embryos by open pulled straw method].
291 embryos (Blastocyst/Morula) from 20 donor sows were vitrified by two step method with OPS (open pulled straw) in solution I (TCM199 + 20% FBS + 10% EG + 10% DMSO) for 3min, and solution II (TCM199 + 20% FBS + 20% EG + 20% DMSO + 0.4mol/L SUC) for 1min, stored in liquid nitrogen for 3 months, and transferred into 8 recipient sows after warming, one recipient sow was pregnant and 8 alive piglets were born. This is the first paper to report getting alive piglets by vitrification in China.
Zhang
De-Fu
DF
Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, China. zhangdefu10@yahoo.com.cn
Liu
Dong
D
Wu
Hua-Li
HL
Zheng
Xiao-Feng
XF
Wang
Zhao-Kai
ZK
Wang
Shao-Bing
SB
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary*; D004624:Embryo Transfer; D005260:Female; D011247:Pregnancy; D013552:Swine / Q000196:embryology*