Long-term room temperature storage of high-quality embryonic stem cell genomic DNA extracted with a simple and rapid procedure.
A very simple procedure for the isolation of high-quality, high-molecular-weight genomic DNA from embryonic stem cells is described. The DNA is very stable once dried and can be stored for long periods of time without refrigeration. Living cells are lysed in a sodium dodecyl sulfate and EDTA buffer containing proteinase K and then air-dried. Samples can be processed in bulk, and an individual can easily process thousands of samples for extraction and shipment on a daily basis using only common laboratory materials such as plastic ware and a multichannel pipetteman.
Tribioli
Carla
C
Institute of Molecular Genetics, C.N.R., Pavia, Italy.
Lufkin
Thomas
T
D000483:Alleles; D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D053595:Embryonic Stem Cells / Q000737:chemistry* / Q000166:cytology*; D017930:Genes, Reporter; D016678:Genome; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009154:Mutation; D016133:Polymerase Chain Reaction; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D049452:Green Fluorescent Proteins; D004247:DNA