title: Acrylamide alters cytoskeletal protein level in rat sciatic nerves.
abstract: Occupational exposure and experimental intoxication with acrylamide (ACR) produce neuropathy characterized by nerve degeneration. To investigate the mechanism of ACR-induced neuropathy, male adult Wistar rats were given ACR (20, 40 mg/kg i.p. 3 days/week) for 8 weeks. Sciatic nerves were Triton-extracted and centrifuged at a high speed (100,000 x g) to yield pellet and supernatant fractions. The contents of six cytoskeletal proteins (NF-L, NF-M, NF-H, alpha-tubulin, beta-tubulin, and beta-actin) in both fractions were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Results showed that the three neurofilament (NF) subunits (NF-L, NF-M, NF-H) in both the pellet and the supernatant fraction decreased significantly (P < 0.01) in the high-dosing group, except for NF-M in the pellet. alpha-tubulin, beta-tubulin, and beta-actin increased significantly in the supernatant (P < 0.01), whereas both alpha-tubulin and beta-tubulin decreased significantly in the pellet (P < 0.01). However, beta-actin was not altered significantly in the sciatic nerves pellet. These findings suggest that ACR altered the cytoskeletal protein level in sciatic nerve, which may be one of the molecular mechanisms of ACR-induced peripheral neuropathy.
authors.affiliation: Institute of toxicology, Shandong University, 44 Wenhua Xi Road, Jinan, 250012, Shandong, P.R. China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Yu
authors.forename: Fuyong
authors.initials: F
authors.lastname: Son
authors.forename: Jinxia
authors.initials: J
authors.lastname: Yu
authors.forename: Xiulan
authors.initials: X
authors.lastname: Zhao
authors.forename: Lihua
authors.initials: L
authors.lastname: Yu
authors.forename: Guozhen
authors.initials: G
authors.lastname: Li
authors.forename: Keqin
authors.initials: K
authors.lastname: Xie
mesh_terms: D020106:Acrylamide / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects* / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D020106:Acrylamide