Expression of germination-related enzymes, CspA, CspB, CspC, SleC, and SleM, of Clostridium perfringens S40 in the mother cell compartment of sporulating cells.
In Clostridium perfringens S40, spore germination-specific enzymes are synthesized during sporulation. Previous reports have demonstrated that two cortex-lytic enzymes, SleC and SleM, and a component of germination-specific protease, CspC, are located outside the cortex as an integral part of the dormant spore. In the present study, we examined the time and compartment of these enzymes' gene expression using reverse transcription-PCR (RT-PCR) and fluorescence microscopy on green fluorescence protein (GFP)-fused proteins. These results suggested that CspABC, SleC, and SleM are synthesized in the mother cell compartment of sporulating cells, probably at stages II approximately III of sporulation, and that the expression of cspABC genes is tricistronic.
Masayama
Atsushi
A
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, Japan.
Hamasaki
Keishi
K
Urakami
Kenji
K
Shimamoto
Seiko
S
Kato
Shiro
S
Makino
Shio
S
Yoshimura
Tohru
T
Moriyama
Michie
M
Moriyama
Ryuichi
R
D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D003016:Clostridium perfringens / Q000201:enzymology* / Q000378:metabolism* / Q000502:physiology; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D006026:Glycoside Hydrolases / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013171:Spores, Bacterial / Q000378:metabolism / Q000502:physiology; D013997:Time Factors
D001426:Bacterial Proteins; D002352:Carrier Proteins; C092058:CspA protein, Clostridium; C102917:CspC protein, Clostridium; D011993:Recombinant Fusion Proteins; C077188:cold-shock protein CspB, Bacteria; D049452:Green Fluorescent Proteins; D006026:Glycoside Hydrolases; C026255:endo-N-acetylmuramidase; D009238:N-Acetylmuramoyl-L-alanine Amidase