title: [The laboratory diagnosis of influenza viruses].
abstract: The methods of the pathogen diagnosis for confirm the infection of the influenza virus, there are the viral isolation and the method of detected the viral antigen. In the each method of inspection, the time of sample extraction, the method of extraction and preservation must be suited to fully improve the sensitivity and specificity. The viral isolation is necessary and indispensable to analyze the antigen of the epidemic viruses and it is the high level of sensitivity. The several methods of detecting the antigen of the gene amplification include the reverse transcription polymerase chain reaction (RT-PCR) method, the loop-mediated isothermal amplification (LAMP) method, the real-time PCR method, and the nucleic acid sequence-based amplification (NASBA) method, and these methods are excellent promptly. As for these detection methods, being chosen according to the purpose is desirable.
authors.affiliation: Department of Microbiology, Nagoya City Public Health Research Institute.
authors.forename: Noriko
authors.initials: N
authors.lastname: Goto
mesh_terms: D000956:Antigens, Viral / Q000302:isolation & purification; D019411:Clinical Laboratory Techniques; D005784:Gene Amplification; D006801:Humans; D007251:Influenza, Human / Q000175:diagnosis; D009975:Orthomyxoviridae / Q000302:isolation & purification*
chemical_list: D000956:Antigens, Viral