[Identification of the gene correlated with salt stress in the Saccharomyces cerevisiae 263-H9 mutant].
The mutant 263-H9 with hypersensitivity to several stress conditions (1.5 mol/L Sorbitol, 0.65 mol/L NaCl and 15 degrees C) was obtained by using transposon mutagenesis in the Saccharomyces cerevisiae strain W303-1A. Unlike other mutants the transposon in 263-H9 was intergenic between GIP2 and YER053C-A. Using gene knockout, a yeast genomic library and other methods, the gene correlated with the salt stress response was identified. The data indicated that the phenotype of 263-H9 was not directly caused by the insertion of the transposon. On the other hand, the hypersensitivity to salt and other stress conditions was due to the deletion of 5 base pairs close to position 936 bp in the PBS2 gene essential for HOG signal pathway regulation under salt stress.
Yu
Dian-Ke
DK
State Key Laboratory of Microbial Technology, Shandong University, Ji'nan 250100, China. yudianking@163.com
Zhang
Xiao-Hua
XH
Liu
Xiang-Yong
XY
Bao
Xiao-Ming
XM
Gao
Dong
D
D004251:DNA Transposable Elements / Q000235:genetics; D015723:Gene Library; D005800:Genes, Fungal; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D017384:Sequence Deletion; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*
D004251:DNA Transposable Elements; D029701:Saccharomyces cerevisiae Proteins; D012965:Sodium Chloride; D020929:Mitogen-Activated Protein Kinase Kinases; C089356:PBS2 protein, S cerevisiae