title: Effects of histologic type on levels of macrophage colony-stimulating factor in liquid contents of benign ovarian tumors.
abstract: BACKGROUND
  Normal ovarian tissue is rich in cytokines. Cytokines are important in the physiology of ovarian function. Most of the same cytokines that are found in normal ovarian tissue are also found in association with benign and malignant tumors in contrast to their functions in normal tissues. Thus, we measured macrophage colony-stimulating factor (M-CSF) levels in the liquid contents of benign ovarian tumors--serous cystadenoma, mucinous cystadenoma, and mature cystic teratoma--and investigated whether M-CSF levels were associated with the histologic type of the ovarian tumors.
  
  
  METHODS
  We enrolled 65 patients, 52 with benign ovarian tumor and 13 in the early postmenopausal period with symptoms of a menopausal disorder. Among the 52 patients with benign ovarian tumor, 16 had serous cystadenoma, 21 had mucinous cystadenoma, and 15 had mature cystic teratoma. Immediately after surgery, the liquid content was drawn from the ovarian tumor, then centrifuged, and the separated supernatant was stored at -30 degrees C. The M-CSF level was determined by the sandwich enzyme-linked immunosorbent assay method with use of three antibodies.
  
  
  RESULTS
  The level of M-CSF was 12,513 U/mL (median) (range, 0-169,000 U/mL) in serous cystadenoma, 915 U/mL (0-82,500 U/mL) in mucinous cystadenoma, and 149 U/mL (0-6,230 U/mL) in mature cystic teratoma. The M-CSF levels increased significantly from mature cystic teratoma to mucinous cystadenoma to serous cystadenoma. The serum M-CSF levels were 308 to 499 U/mL in patients with benign ovarian tumor. The M-CSF levels did not differ significantly among the three groups. The serum M-CSF levels were 162 U/mL (0-473 U/mL) in menopausal patients.
  
  
  CONCLUSIONS
  Elevation of levels of M-CSF varies according to histologic type in benign ovarian tumors. This implies that the antitumor activities of M-CSF for serous cystadenoma, mucinous cystadenoma, and mature cystic teratoma differ by histologic type.
authors.affiliation: Department of Obstetrics and Gynecology, International University of Health and Welfare Atami Hospital, Shizuoka, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Hayashi
authors.forename: Mitsuei
authors.initials: M
authors.lastname: Shibazaki
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Sohma
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Inaba
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D045604:Extracellular Fluid / Q000378:metabolism*; D005260:Female; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D009928:Organ Specificity; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: D009363:Neoplasm Proteins; D016173:Macrophage Colony-Stimulating Factor