title: [Deterimination of schisandrin in Kadsura heteroclita (Roxb.) Craib by RP-HPLC].
abstract: OBJECTIVE
  To develop a method of RP-HPLC for determination of schisandrin content in Kadsura heteroclita (Roxb.) Craib.
  
  
  METHODS
  RP-HPLC analysis was carried out by using Kromasil C18 column (5 microm, 250 mm x 4.6 mm) and methanol-water (65:35) as the mobile, and the detection wavelength was 250 nm.
  
  
  RESULTS
  The method was linear in the range of 0.08-0.06 nicrog (r = 0.9998), and the average recovery was 100.24%, with the RSD of 2.09% (n=5).
  
  
  CONCLUSIONS
  The method was simple, accurate, highly sensitive and reproducible. It may be used for the quantitative determination of schisandrin in extract of Kadsura heteroclita (Roxb.) Craib.
authors.affiliation: Jinan University, Guangzhou 510632, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Weng
authors.forename: Qin
authors.initials: Q
authors.lastname: Gao
authors.forename: Xiao-guang
authors.initials: XG
authors.lastname: Li
authors.forename: Huan-min
authors.initials: HM
authors.lastname: Luo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D034242:Cyclooctanes / Q000032:analysis* / Q000302:isolation & purification; D032135:Kadsura / Q000737:chemistry* / Q000145:classification; D017705:Lignans / Q000032:analysis* / Q000302:isolation & purification; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011083:Polycyclic Compounds / Q000032:analysis* / Q000302:isolation & purification; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; C011105:schizandrin