Mass spectrometric detection of tissue proteins in plasma.
It has long been thought that blood plasma could serve as a window into the state of one's organs in health and disease because tissue-derived proteins represent a significant fraction of the plasma proteome. Although substantial technical progress has been made toward the goal of comprehensively analyzing the blood plasma proteome, the basic assumption that proteins derived from a variety of tissues could indeed be detectable in plasma using current proteomics technologies has not been rigorously tested. Here we provide evidence that such tissue-derived proteins are both present and detectable in plasma via direct mass spectrometric analysis of captured glycopeptides and thus provide a conceptual basis for plasma protein biomarker discovery and analysis.
Zhang
Hui
H
Institute for Systems Biology, Seattle, Washington 98103, USA. hzhang23@jhmi.edu
Liu
Alvin Y
AY
Loriaux
Paul
P
Wollscheid
Bernd
B
Zhou
Yong
Y
Watts
Julian D
JD
Aebersold
Ruedi
R
D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D006020:Glycopeptides / Q000097:blood* / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000097:blood* / Q000737:chemistry
D015703:Antigens, CD; D006020:Glycopeptides; D009363:Neoplasm Proteins