title: [Methylation status of FHIT gene in plasma and expression of FHIT gene in cancer tissue of cervical cancer patients].
abstract: OBJECTIVE
  To explore the methylation status of 5' CpG island of fragile histidine triad (FHIT) gene in plasma and the expression of FHIT protein in cancer tissue of cervical cancer patients.
  
  
  METHODS
  Methylation-specific PCR (MS-PCR) was employed to examine methylation of FHIT gene in 151 plasma samples before treatment. The immunohistochemistry was used to the expression of FHIT protein in cancer tissues.
  
  
  RESULTS
  CpG island methylation of FHIT was detected in 31.13% of plasma samples. The expression of FHIT protein was decreased or discarded in 59.60% of cervical cancer tissues. Among them 47.78% was included in methylation positive samples.
  
  
  CONCLUSIONS
  CpG island methylation of FHIT gene in plasma plays an important role on cervical cancer, which results in decreased expression of FHIT protein. It can be used to diagnose and evaluate the effect of treatment to cervical cancers.
authors.affiliation: Department of Genetics, the Central Hospital of Gynecology and Obstetric, Tianjin, 300052 PR China.
authors.forename: Chen-chun
authors.initials: CC
authors.lastname: Ren
authors.forename: Xu-hong
authors.initials: XH
authors.lastname: Miao
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Rui
authors.initials: R
authors.lastname: Sun
authors.forename: Wen-qin
authors.initials: WQ
authors.lastname: Song
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D000368:Aged; D019175:DNA Methylation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase