title: Inhibitory effects of a specific phage-displayed peptide on high peritoneal metastasis of gastric cancer.
abstract: Peritoneal dissemination in gastric cancer is the most frequent cause of the noncurative resection and recurrence after curative resection. We, therefore, evaluated the feasibility of a peptide, which was obtained by screening a random phage display library, in the treatment of peritoneal metastases of gastric cancer. In this study, a novel cell line, GC9811-P, with a high potential peritoneal metastasis of gastric cancer derived from its parental cell line, GC9811, was established. Using a phage display library, we isolated a specific peptide that selectively bound to GC9811-P cells rather than its parental GC9811cells. The isolated phage-displaying peptide, SMSIASPYIALE (named peptide PIII), was obtained after four rounds of selection, showing a tendency to preferentially bind to GC9811-P cells compared with a panel of other gastric cancer cell lines, and preferentially accumulate in peritoneal metastasis tumor tissue in comparison with control organs, peritoneum, liver, pancreas, spleen, lung, and kidney. Further study showed that synthetic peptide PIII could significantly inhibit adhesive and invasional ability of GC9811-P cells and could effectively block the corresponding phage binding to the GC9811-P cells, whereas, exposure of the cells to various concentrations of peptide PIII showed no obvious cell growth inhibition. Furthermore, a highly reproducible animal experimental model of gastric cancer with peritoneal dissemination was established in nude mice by injecting a suspension of the cell line into the gastric wall of nude mice. Animals intraperitoneally treated with peptide PIII in this model or another animal model of gastric cancer with peritoneal dissemination established using MKN45 cells showed suppressed tumor metastasis to peritoneum and significantly prolonged survival. In conclusion, the selected peptide PIII was a biologically active peptide and could effectively inhibit peritoneal dissemination of gastric cancer.
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi Province 710032, China.
authors.forename: Feihu
authors.initials: F
authors.lastname: Bai
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Shi
authors.forename: Kedong
authors.initials: K
authors.lastname: Zhang
authors.forename: Shuhui
authors.initials: S
authors.lastname: Liang
authors.forename: Liu
authors.initials: L
authors.lastname: Hong
authors.forename: Huihong
authors.initials: H
authors.lastname: Zhai
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Lu
authors.forename: Yu
authors.initials: Y
authors.lastname: Han
authors.forename: Fang
authors.initials: F
authors.lastname: Yin
authors.forename: Kaichun
authors.initials: K
authors.lastname: Wu
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D009362:Neoplasm Metastasis / Q000188:drug therapy* / Q000517:prevention & control; D009368:Neoplasm Transplantation; D019151:Peptide Library; D010455:Peptides / Q000494:pharmacology / Q000627:therapeutic use*; D010534:Peritoneal Neoplasms / Q000188:drug therapy / Q000556:secondary*; D013274:Stomach Neoplasms / Q000473:pathology*; D015996:Survival Rate
chemical_list: D019151:Peptide Library; D010455:Peptides