title: Monitoring of stool microbiota in subjects with diarrhea indicates distortions in composition.
abstract: We utilized denaturing gradient gel electrophoresis profiling to survey stool microbiota in 175 persons with diarrhea and 113 asymptomatic persons in a diarrhea surveillance study. Compared with healthy controls, the microbiota profiles in diarrhea cases more frequently exhibited decreased diversity and strong bands indicating the overgrowth of selected bacteria or bacterial groups.
authors.affiliation: Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Rm. 934-B MSTF, 10 S. Pine St., Baltimore, MD 21201, USA. vmai@epi.umaryland.edu
authors.forename: Volker
authors.initials: V
authors.lastname: Mai
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Braden
authors.forename: Jill
authors.initials: J
authors.lastname: Heckendorf
authors.forename: Baiba
authors.initials: B
authors.lastname: Pironis
authors.forename: Jon Mark
authors.initials: JM
authors.lastname: Hirshon
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S