title: Introduction of the anti-apoptotic baculovirus p35 gene in passion fruit induces herbicide tolerance, reduced bacterial lesions, but does not inhibits passion fruit woodiness disease progress induced by cowpea aphid-borne mosaic virus (CABMV).
abstract: The introduction of anti-apoptotic genes into plants leads to resistance to environmental stress and broad-spectrum disease resistance. The anti-apoptotic gene (p35) from a baculovirus was introduced into the genome of passion fruit plants by biobalistics. Eleven regenerated plants showed the presence of the p35 gene by PCR and/or dot blot hybridization. Transcriptional analysis of regenerated plants showed the presence of specific p35 transcripts in 9 of them. Regenerated plants containing the p35 gene were inoculated with the cowpea aphid-borne mosaic virus (CABMV), the bacterium Xanthomonas axonopodis pv passiflorae, and the herbicide, glufosinate, (Syngenta). None of the plants showed resistance to CABMV. Regenerated plants (p35+) showed less than half of local lesions showed by non-transgenic plants when inoculated with X. axonopodis and some p35+ plants showed increased tolerance to the glufosinate herbicide when compared to non-transgenic plants.
authors.affiliation: Departamento de Biologia Celular, Univesidade de Brasília, Brasília, Brasil.
authors.forename: Daniele Scandiucci
authors.initials: DS
authors.lastname: de Freitas
authors.forename: Marly C Felipe
authors.initials: MC
authors.lastname: Coelho
authors.forename: Manoel T
authors.initials: MT
authors.lastname: Souza
authors.forename: Abi
authors.initials: A
authors.lastname: Marques
authors.forename: E Bergmann Morais
authors.initials: EB
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D017798:Comovirus / Q000502:physiology*; D024861:Genetic Enhancement / Q000379:methods*; D053209:Herbicide Resistance / Q000502:physiology*; D029598:Passiflora / Q000382:microbiology / Q000502:physiology* / Q000821:virology*; D010935:Plant Diseases / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D014764:Viral Proteins; C492234:p35 protein, Baculovirus