Protein P200 is dispensable for Mycoplasma pneumoniae hemadsorption but not gliding motility or colonization of differentiated bronchial epithelium.
Mycoplasma pneumoniae protein P200 was localized to the terminal organelle, which functions in cytadherence and gliding motility. The loss of P200 had no impact on binding to erythrocytes and A549 cells but resulted in impaired gliding motility and colonization of differentiated bronchial epithelium. Thus, gliding may be necessary to overcome mucociliary clearance.
Jordan
Jarrat L
JL
Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
Chang
How-Yi
HY
Balish
Mitchell F
MF
Holt
Lynley S
LS
Bose
Stephanie R
SR
Hasselbring
Benjamin M
BM
Waldo
Robert H
RH
Krunkosky
Thomas M
TM
Krause
Duncan C
DC
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D015153:Blotting, Western; D001980:Bronchi / Q000276:immunology / Q000382:microbiology; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D006382:Hemadsorption / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009079:Mucociliary Clearance / Q000276:immunology; D009177:Mycoplasma pneumoniae / Q000472:pathogenicity* / Q000502:physiology / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D020545:Respiratory Mucosa / Q000276:immunology / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D008565:Membrane Proteins; C099985:P200 protein, Mycoplasma pneumoniae