title: Antiangiogenic activity of a concentrated effective microorganism fermentation extract.
abstract: We have previously demonstrated the possible growth inhibitory activity of both first generation of the effective microorganism fermentation extract (EM-X) as well as the second generation (EM-X2) on cancer cell lines in vitro. The possible anti-angiogenic potential of EM-X has not been reported. Herein we show that using the concentrated EM-X, the growth of human umbilical cord endothelial cells (HUCE) was significantly inhibited in vitro. Enzyme linked immunosorbent assay suggested that the concentrated EM-X is able to reduce the level of vascular endothelial growth factor (VEGF) from Hep3B hepatocellular carcinoma (HCC) cells. The conditioned culture medium obtained from the concentrated EM-X incubated Hep3B HCC cells possessed significant antiproliferative effect on the HUCE cells. Moreover, in vivo chick chorioallantoic membrane assay further demonstrated that the concentrated EM-X is able to greatly inhibit the basic fibroblast growth factor induced angiogenesis from chick embryo experiment. We speculate that the anti-cancer potential of this concentrated EM-X involved growth inhibition on cancer cell and antiangiogenic effect on HUCE cells.
authors.affiliation: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, PR China.
authors.forename: Chung Hin
authors.initials: CH
authors.lastname: Chui
authors.forename: Roberto
authors.initials: R
authors.lastname: Gambari
authors.forename: Fung Yi
authors.initials: FY
authors.lastname: Lau
authors.forename: Desmond Kwok Po
authors.initials: DK
authors.lastname: Hau
authors.forename: Raymond Siu Ming
authors.initials: RS
authors.lastname: Wong
authors.forename: Gregory Yin Ming
authors.initials: GY
authors.lastname: Cheng
authors.forename: Stanton Hon Lung
authors.initials: SH
authors.lastname: Kok
authors.forename: Teruo
authors.initials: T
authors.lastname: Higa
authors.forename: Bin
authors.initials: B
authors.lastname: Ke
authors.forename: Albert Sun Chi
authors.initials: AS
authors.lastname: Chan
authors.forename: David Wan Fun
authors.initials: DW
authors.lastname: Fong
authors.forename: Johnny Cheuk On
authors.initials: JC
authors.lastname: Tang
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002642:Chick Embryo; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned; D015536:Down-Regulation; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D014470:Umbilical Cord / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000037:antagonists & inhibitors*
chemical_list: D020533:Angiogenesis Inhibitors; D017077:Culture Media, Conditioned; D010936:Plant Extracts; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C508178:microorganism fermentation extract