title: Absence of Gup1p in Saccharomyces cerevisiae results in defective cell wall composition, assembly, stability and morphology.
abstract: Saccharomyces cerevisiae Gup1p and its homologue Gup2p, members of the superfamily of membrane-bound O-acyl transferases, were previously associated with glycerol-mediated salt-stress recovery and glycerol symporter activity. Several other phenotypes suggested Gup1p involvement in processes connected with cell structure organization and biogenesis. The gup1Delta mutant is also thermosensitive and exhibits an altered plasma membrane lipid composition. The present work shows that the thermosensitivity is independent of glycerol production and retention. Furthermore, the mutant grows poorly on salt, ethanol and weak carboxylic acids, suggestive of a malfunctioning membrane potential. Additionally, gup1Delta is sensitive to cell wall-perturbing agents, such as Calcofluor white, Zymolyase, lyticase and sodium dodecyl sulphate and exhibits a sedimentation/aggregation phenotype. Quantitative analysis of cell wall components yielded increased contents of chitin and beta-1,3-glucans and lower amounts of mannoproteins. Consistently, scanning electron microscopy showed a strikingly rough surface morphology of the mutant cells. These results suggest that the gup1Delta is affected in cell wall assembly and stability, although the Slt2p/MAP kinase from the PKC pathway was phosphorylated during hypo-osmotic shock to a normal extent. Results emphasize the pleiotropic nature of gup1Delta, and are consistent with a role of Gulp1p in connection with several pathways for cell maintenance and construction/remodelling.
authors.affiliation: Carlsberg Laboratory, Copenhagen Valby, Denmark.
authors.forename: Célia
authors.initials: C
authors.lastname: Ferreira
authors.forename: Sónia
authors.initials: S
authors.lastname: Silva
authors.forename: Frank
authors.initials: F
authors.lastname: van Voorst
authors.forename: Cristina
authors.initials: C
authors.lastname: Aguiar
authors.forename: Morten C
authors.initials: MC
authors.lastname: Kielland-Brandt
authors.forename: Anders
authors.initials: A
authors.lastname: Brandt
authors.forename: Cândida
authors.initials: C
authors.lastname: Lucas
mesh_terms: D002110:Caffeine / Q000494:pharmacology; D002473:Cell Wall / Q000737:chemistry* / Q000648:ultrastructure; D008562:Membrane Glycoproteins / Q000032:analysis; D026901:Membrane Transport Proteins / Q000502:physiology*; D009154:Mutation; D011493:Protein Kinase C / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D047071:beta-Glucans / Q000032:analysis
chemical_list: C414103:Gup1 protein, S cerevisiae; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D047071:beta-Glucans; C025363:mannoproteins; D002110:Caffeine; D011493:Protein Kinase C