Innotrac Aio!: a point-of-care or a routine analyzer? Analytical performance and plasma/whole blood comparison.
METHODS
The aim of the present study was to evaluate the analytical performance of the Innotrac Aio! analyzer in measuring the cardiac markers troponin I (TnI), myoglobin (Myo) and creatine kinase-MB (CK-MB), both in lithium heparin plasma and in whole blood.


RESULTS
TnI analytical sensitivity was 0.012 microg/L and the concentration corresponding to CV=10% was 0.036 microg/L. In healthy subjects, the 99th percentile TnI values were 0.023 and 0.016 microg/L in whole blood and in plasma, respectively. One hundred samples were tested both in whole blood and in plasma: TnI-whole-blood=1.16-plasma+0.011, bias=+0.18 (95% CI from -0.22 to +0.59); Myo-whole-blood=1.04-plasma-1.93, bias=+1.08 (95% CI from -6.17 to +8.32); CK-MB-whole-blood=1.11-plasma-0.09, bias=+0.96 (95% CI from -0.53 to +2.45). The method comparison with the RxL Dimension analyzer for TnI and Myo gave the following results: TnI Aio!=0.30 TnI RxL+0.00; bias=-5.80 (95% CI from -7.81 to -3.78), Myo Aio!=1.33 Myo RxL+0.42, bias=+53.09 (95% CI from +38.44 to +67.74).


CONCLUSIONS
The analytical performance of the Innotrac Aio! analyzer was satisfactory for all three cardiac markers evaluated and, in particular, the TnI method provided sensitive and accurate results. The most important finding in this study is the possibility to perform the tests as either routine or point-of-care analysis, thus overcoming the variability of results obtained employing different methods.
Altinier
Sara
S
Department of Laboratory Medicine, University-Hospital of Padova, Padova, Italy. sara.altinier@sanita.padova.it
Zaninotto
Martina
M
Mion
Monica Maria
MM
Plebani
Mario
M
D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D052279:Creatine Kinase, MB Form / Q000097:blood; D005470:Fluorometry / Q000295:instrumentation*; D006331:Heart Diseases / Q000097:blood* / Q000175:diagnosis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D009211:Myoglobin / Q000097:blood; D010949:Plasma / Q000737:chemistry*; D019095:Point-of-Care Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019210:Troponin I / Q000097:blood
D015415:Biomarkers; D001798:Blood Proteins; D009211:Myoglobin; D019210:Troponin I; D052279:Creatine Kinase, MB Form