title: Efficient and scalable method in isolation of polymethoxyflavones from orange peel extract by supercritical fluid chromatography.
abstract: Polymethoxyflavones (PMFs) from citrus genus are of particular interest because of their broad spectrum of biological activities, such as anti-inflammatory, anti-carcinogenic, and anti-atherogenic properties. Recently, the exploration into the beneficial health properties of PMFs in citrus fruits has dramatically increased. However, the supply of pure PMFs in the in vivo study is a limiting factor due to the difficulties in large-scale isolation of the interested PMFs. Therefore, the development of an efficient and a scalable separation method of PMFs is necessary and significant. In this paper, we report a newly developed method for efficient and relatively large-scale isolation of four PMFs from sweet orange (Citrus sinensis) peel by employing supercritical chromatography (SFC): nobiletin, tangeretin, 3,5,6,7,8,3',4'-heptamethoxyflavone and 5,6,7,4'-tetramethoxyflavone.
authors.affiliation: Department of Drug Discovery, Hoffmann-La Roche Inc., Nutley, NJ 07110, USA. shiming.li@roche.com
authors.forename: Shiming
authors.initials: S
authors.lastname: Li
authors.forename: Ted
authors.initials: T
authors.lastname: Lambros
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.forename: Robert
authors.initials: R
authors.lastname: Goodnow
authors.forename: Chi-Tang
authors.initials: CT
authors.lastname: Ho
mesh_terms: D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D032084:Citrus sinensis / Q000737:chemistry*; D047309:Flavones / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D047309:Flavones