Blood cell separation using amphiphilic copolymers containing N,N-dimethylacrylamide.
To develop leukocyte removal filters effective for whole blood, amphiphilic copolymers based on N,N-dimethylacrylamide were synthesized and evaluated as coating materials for poly(ethylene terephthalate) filters. The copolymers with methyl methacrylate or 2-hydroxypropyl methacrylate as a comonomer showed higher platelet permeation ratios (more than 90%) than that of the copolymer with n-butyl methacrylate, though the logarithmic reductions of leukocytes by these copolymers were less than four. An increase in the platelet permeation for whole blood tended to increase the leukocyte permeation. The permeation of both platelets and leukocytes increased with the amount of copolymer coated on the filters because of the change in the physical properties such as the average pore size, total surface area, and total pore volume of coated filters. These results confirm that both the chemical and physical properties of the filters play important roles to control the permeation behavior.
Natori
Shizue Hayashi
SH
Department of Materials and Life Science, Faculty of Science and Technology, Seikei University, 3-3-1 Kichijoji Kitamachi, Musashino, Tokyo 180-8633, Japan. shizue_natori@yahoo.co.jp
Kurita
Keisuke
K
D000178:Acrylamides; D001773:Blood Cells / Q000166:cytology*; D001792:Blood Platelets / Q000166:cytology; D002469:Cell Separation / Q000379:methods*; D020099:Coated Materials, Biocompatible; D005374:Filtration; D006801:Humans; D066298:In Vitro Techniques; D047589:Leukocyte Reduction Procedures; D008422:Materials Testing; D013501:Surface-Active Agents
D000178:Acrylamides; D020099:Coated Materials, Biocompatible; D013501:Surface-Active Agents; C099046:N,N-dimethylacrylamide