[Stress-protective effect of the synthetic ACTH-like peptide leucocorticotropin].
We found that the tritium-labeled synthetic ACTH-like octapeptide leucocorticotropin corresponding to the 81-88 sequence of the precursor of human interleukin-1alpha ([3H]GKVLKKRR) is bound by the ACTH receptor of rat adrenal cortex with a high affinity and specificity (Kd 2.2 +/- 0.1 nM). This peptide was shown to exert no effect on the adenylate cyclase activity of the membranes of rat adrenal cortex in the concentration range from 1 to 1000 nM. Leucocorticotropin administration three times at doses of 10-20 microg/animal did not change the level of hydroxycorticosteroids (11-HOCS) in the rat adrenal glands in the absence of temperature action. At the same time, the peptide abolishes (at a dose of 20 microg/animal, three times) or significantly decreases (at a dose of 10 microg/animal, three times) the dramatic increase in the 11-HOCS content in the adrenal glands occurring in the case of cold or heat shock. Thus, leucocorticotropin normalizes the 11-HOCS level in the rat adrenal cortex during stress. The stress-protective effect of the peptide is mediated through the ACTH receptor.
Vanina
V I
VI
Kovalitskaia
Iu A
IuA
Kolobov
A A
AA
Kampe-Nemm
E A
EA
Zolotarev
Iu A
IuA
Iurovskiĭ
V V
VV
Lipkin
V M
VM
Navolotskaia
E V
EV
D000281:Administration, Intranasal; D000302:Adrenal Cortex / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D000305:Adrenal Cortex Hormones / Q000032:analysis / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D053582:Interleukin-1alpha / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D018043:Receptors, Corticotropin / Q000819:agonists* / Q000378:metabolism; D013312:Stress, Physiological / Q000517:prevention & control*
D000305:Adrenal Cortex Hormones; D053582:Interleukin-1alpha; D010446:Peptide Fragments; D020011:Protective Agents; D018043:Receptors, Corticotropin; C515410:leucocorticotropin; D000324:Adrenocorticotropic Hormone