Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis.
The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.
Mueller
Markus
M
University of Konstanz, Biochemical Pharmacology, 78457 Konstanz, Germany.
Bunk
Sebastian
S
Diterich
Isabel
I
Weichel
Michael
M
Rauter
Carolin
C
Hassler
Dieter
D
Hermann
Corinna
C
Crameri
Reto
R
Hartung
Thomas
T
D000328:Adult; D025065:Borrelia burgdorferi / Q000235:genetics*; D006801:Humans; D008193:Lyme Disease / Q000175:diagnosis*; D008875:Middle Aged; D019151:Peptide Library; D012269:Ribosomal Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D019151:Peptide Library; D012269:Ribosomal Proteins; C031127:ribosomal protein L25