title: Analysis of oxygen binding by hemoglobin on the basis of mean intrinsic thermodynamic quantities.
abstract: The binding data for oxygenation of human hemoglobin, Hb, at various temperatures and in the absence and presence of 2,3-diphosphoglycerate, DPG, and inositol hexakis phosphate, IHP, were analyzed for extraction of mean intrinsic Gibbs free energy, DeltaGo, enthalpy, DeltaHo, and entropy, DeltaSo, of binding at various partial oxygen pressures. This method of analysis considers all the protein species present such as dimer and tetramer forms which were not considered by Imai et al. (Imai K et al., 1970, Biochim Biophys Acta 200: 189-196), in their analysis which was based on Adair equation. In this regard, the values of Hill equation parameters were estimated with high precision at all points of the binding curve and used for calculation of DeltaGo, DeltaHo and DeltaSo were also calculated by analysis of DeltaGo values at various temperatures using van't Hoff equation. The results represent the enthalpic nature of the cooperativity in Hb oxygenation and the compensation effect of intrinsic entropy. The interpretation of results also to be, into account the decrease of the binding affinity of sites for oxygen in the presence of DPG and IHP without any considerable changes in the site-site interaction (extent of cooperativity). In other words, the interactions between bound ligands, organic phosphates and oxygen, are more due to a decreasing binding affinity and not to the reduction of the cooperative interaction between sites. The results also document the more heterotropic effect of IHP compared to DPG.
authors.affiliation: Laboratory of Biophysical Chemistry, Department of Chemistry, Isfahan University, Isfahan, Iran. bordbar@chem.ui.ac.ir
authors.forename: Abdol-Khalegh
authors.initials: AK
authors.lastname: Bordbar
authors.forename: Sayed Habib-Allah
authors.initials: SH
authors.lastname: Mousavi
authors.forename: Hamid
authors.initials: H
authors.lastname: Dazhampanah
mesh_terms: D019794:2,3-Diphosphoglycerate / Q000737:chemistry; D000494:Allosteric Regulation; D002151:Calorimetry; D019277:Entropy; D006441:Hemoglobin A / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008433:Mathematics; D008954:Models, Biological; D008956:Models, Chemical; D010100:Oxygen / Q000097:blood* / Q000737:chemistry; D010108:Oxyhemoglobins / Q000737:chemistry / Q000378:metabolism*; D010313:Partial Pressure; D010833:Phytic Acid; D011485:Protein Binding / Q000502:physiology; D012107:Research Design; D013816:Thermodynamics; D014894:Weights and Measures
chemical_list: D010108:Oxyhemoglobins; D019794:2,3-Diphosphoglycerate; D010833:Phytic Acid; D006441:Hemoglobin A; D010100:Oxygen