title: Ngoye virus: a novel evolutionary lineage within the genus Flavivirus.
abstract: By using degenerate primers deduced from conserved patterns in the flavivirus polymerase gene, a novel RNA virus was discovered in Rhipicephalus ticks sampled from members of the family Bovidae in Senegal. It was named Ngoye virus (NGOV) after the location from which it was isolated. Viral particles could be observed by electron microscopy, but isolation in vertebrate or invertebrate cell lines or by intracerebral infection of newborn mice remained unsuccessful. This is atypical of recognized arboviruses. The characterization of 4176 nt of the non-structural genes revealed that NGOV is a novel flavivirus species. It forms a distinct phylogenetic lineage related distantly to previously identified members of the genus Flavivirus. Analysis of genetic data suggested that the processing of the NGOV polyprotein and the organization of its replication complex are similar to those of flaviviruses. Together with other recent data, these findings suggest that a large number of viruses related distantly to 'classical' arthropod-borne flaviviruses remain to be discovered.
authors.affiliation: Unité des Virus Emergents (EA3292, IFR48, IRD UR0178), Faculté de Médecine La Timone, 27 boulevard Jean Moulin, 13005 Marseille, France.
authors.forename: Gilda
authors.initials: G
authors.lastname: Grard
authors.affiliation: IRD - UR0178, Conditions et Territoires d'Emergence des Maladies, BP 1386, 18524 Dakar, Senegal.|Unité des Virus Emergents (EA3292, IFR48, IRD UR0178), Faculté de Médecine La Timone, 27 boulevard Jean Moulin, 13005 Marseille, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Lemasson
authors.affiliation: IRD - UR0178, Conditions et Territoires d'Emergence des Maladies, BP 1386, 18524 Dakar, Senegal.
authors.forename: Massamba
authors.initials: M
authors.lastname: Sylla
authors.affiliation: IRD - UR0178, Mahidol University, Research Center for Emerging Viral Diseases/Center for Vaccine Development Institute of Sciences, Salaya, 25/25 Phutthamonthon 4, Nakhonpathom 73170, Thailand.
authors.forename: Audrey
authors.initials: A
authors.lastname: Dubot
authors.affiliation: Department of Zoology, University of Oxford, Oxford, UK.
authors.forename: Shelley
authors.initials: S
authors.lastname: Cook
authors.affiliation: IRD - UR0178, Conditions et Territoires d'Emergence des Maladies, BP 1386, 18524 Dakar, Senegal.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Molez
authors.affiliation: Centre International de Recherches Médicales de Franceville, BP 769 Franceville, Gabon.
authors.forename: Xavier
authors.initials: X
authors.lastname: Pourrut
authors.affiliation: Unité des Virus Emergents (EA3292, IFR48, IRD UR0178), Faculté de Médecine La Timone, 27 boulevard Jean Moulin, 13005 Marseille, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Charrel
authors.affiliation: IRD - UR0178, Mahidol University, Research Center for Emerging Viral Diseases/Center for Vaccine Development Institute of Sciences, Salaya, 25/25 Phutthamonthon 4, Nakhonpathom 73170, Thailand.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Gonzalez
authors.affiliation: Department of Entomology, University of Minnesota, St Paul, MN 55108, USA.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Munderloh
authors.affiliation: Department of Biology, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Edward C
authors.initials: EC
authors.lastname: Holmes
authors.affiliation: Unité des Virus Emergents (EA3292, IFR48, IRD UR0178), Faculté de Médecine La Timone, 27 boulevard Jean Moulin, 13005 Marseille, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: de Lamballerie
mesh_terms: D000818:Animals; D002417:Cattle / Q000469:parasitology; D012321:DNA-Directed RNA Polymerases; D005416:Flavivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005814:Genes, Viral; D006041:Goats / Q000469:parasitology; D026863:Ixodidae / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020815:Polyproteins / Q000235:genetics; D020365:RNA Helicases / Q000235:genetics; D012675:Senegal; D012697:Serine Endopeptidases / Q000235:genetics; D012756:Sheep / Q000469:parasitology; D013045:Species Specificity; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D012321:DNA-Directed RNA Polymerases; D020815:Polyproteins; D020365:RNA Helicases; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C096041:NS5 protein, flavivirus