title: In vitro percutaneous absorption and in vivo stratum corneum distribution of an organic and a mineral sunscreen.
abstract: Sunscreens, whose main function is to protect the skin against the harmful effects of solar irradiation, should remain at the skin surface or impregnate the first layers of the stratum corneum only and not penetrate into the underlying living tissue. The goal of this work was to assess the penetration of titanium dioxide (TiO(2)) and methylene bis-benzotriazoyl tetramethylbutylphenol (MBBT), included in a broad-spectrum sunscreen formulation, into human skin in vivo, using the tape stripping method, and in vitro, using a compartmental approach. An additional objective was to propose an easy and minimally invasive approach to determining the percutaneous uptake of sunscreens following topical application. TiO(2) and MBBT were quantified using colorimetric assay and HPLC analysis, respectively. The transmission electron microscopy and particle-induced X-ray emission techniques were used to localize the TiO(2) in skin sections. More than 90% of both sunscreens was recovered in the first 15 tape strippings. In addition we have shown that the remaining 10% did not penetrate the viable tissue, but was localized in the furrows and in the opened infundibulum. Less than 0.1% of MBBT was detected in the receptor medium, and no TiO(2) was detected in the follicle, viable epidermis or dermis. Thus, this in vivo and in vitro penetration study showed an absence of TiO(2) penetration into the viable skin layers through either transcorneal or transfollicular pathways and negligible transcutaneous absorption of MBBT. However, differences in distribution within the stratum corneum reinforced the need for a complementary approach, using minimally invasive in vivo methodology and in vitro compartmental analysis. This combination represents a well-adapted method for testing the safety of topically applied sunscreen formulations in real-life conditions.
authors.affiliation: Laboratoire de Pharmacocinétique Cutanée, Institut de Recherche Pierre Fabre, Castanet-Tolosan, France. alain.mavon@pierre-fabre.com
authors.forename: A
authors.initials: A
authors.lastname: Mavon
authors.forename: C
authors.initials: C
authors.lastname: Miquel
authors.forename: O
authors.initials: O
authors.lastname: Lejeune
authors.forename: B
authors.initials: B
authors.lastname: Payre
authors.forename: P
authors.initials: P
authors.lastname: Moretto
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D005260:Female; D006801:Humans; D046529:Microscopy, Electron, Transmission / Q000379:methods; D012869:Skin Absorption / Q000528:radiation effects*; D013052:Spectrometry, X-Ray Emission; D013473:Sunscreening Agents / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014025:Titanium / Q000493:pharmacokinetics*; D014230:Triazoles / Q000493:pharmacokinetics*
chemical_list: C510891:2,2'-methylene-bis-(6-(2H-benzotriazole-2-yl))-4-(1,1,3,3-tetramethylbutyl)phenol; D013473:Sunscreening Agents; D014230:Triazoles; C009495:titanium dioxide; D014025:Titanium