title: Crystallization and preliminary X-ray analysis of Salmonella FliI, the ATPase component of the type III flagellar protein-export apparatus.
abstract: Most of the structural components making up the bacterial flagellum are translocated through the central channel of the growing flagellar structure by the type III flagellar protein-export apparatus in an ATPase-driven manner and are assembled at the growing end. FliI is the ATPase that drives flagellar protein export using the energy of ATP hydrolysis. FliI forms an oligomeric ring structure in order to attain maximum ATPase activity. In this study, FliI(Delta1-18), an N-terminally truncated variant of FliI lacking the first 18 residues, was purified and crystallized. Crystals were obtained using the hanging-drop vapour-diffusion technique with PEG 8000 as a precipitant. FliI(Delta1-18) crystals grew in the monoclinic space group P2(1), with unit-cell parameters a = 48, b = 73, c = 126 A, beta = 94 degrees, and diffracted to 2.4 A resolution. Anomalous difference Patterson maps of Os-derivative and Pt-derivative crystals showed significant peaks in their Harker sections, indicating that both derivatives are suitable for structure determination.
authors.affiliation: Dynamic NanoMachine Project, ICORP, JST, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Minamino
authors.forename: Katsumi
authors.initials: K
authors.lastname: Imada
authors.forename: Aiko
authors.initials: A
authors.lastname: Tahara
authors.forename: May
authors.initials: M
authors.lastname: Kihara
authors.forename: Robert M
authors.initials: RM
authors.lastname: Macnab
authors.forename: Keiichi
authors.initials: K
authors.lastname: Namba
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D021381:Protein Transport; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000235:genetics; D012486:Salmonella typhimurium / Q000737:chemistry / Q000201:enzymology*; D012995:Solubility
chemical_list: D001426:Bacterial Proteins; C069251:fliI protein, bacteria; D006180:Proton-Translocating ATPases