Opium Poppy (Papaver somniferum).
The genetic transformation of opium poppy, Papaver somniferum, offers the opportunity to study the mechanisms involved in the regulation of benzylisoquinoline and morphinan alkaloid biosynthesis. The development of an efficient transformation protocol for opium poppy has allowed us to transform a range of genotypes from all around the world, including previously recalcitrant high-yielding commercial Australian cultivars. The method involves Agrobacterium tumefaciens infection of hypocotyl explants, followed by the production of antibiotic or herbicide resistant embryogenic callus, the subsequent induction of somatic embryos and development into normal plants. The use of different selective agents, binary vectors, and poppy genotypes has demonstrated the robustness and reliability of this protocol in the production of many hundreds of confirmed transgenic poppies.
Chitty
Julie A
JA
CSIRO, Division of Plant Industry, GPO Box 1600, Canberra ACT 2601, Australia.
Allen
Robert S
RS
Larkin
Philip J
PJ
D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D005822:Genetic Vectors; D005838:Genotype; D018525:Germination; D006540:Herbicides / Q000494:pharmacology; D018546:Hypocotyl / Q000235:genetics / Q000502:physiology; D010207:Papaver / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D010303:Paromomycin / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000254:growth & development; D012038:Regeneration; D012639:Seeds / Q000235:genetics / Q000502:physiology; D012987:Soil; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D006540:Herbicides; D012987:Soil; D010303:Paromomycin