title: Detection of norovirus antigens from recombinant virus-like particles and stool samples by a commercial norovirus enzyme-linked immunosorbent assay kit.
abstract: The commercial norovirus enzyme-linked immunosorbent assay kit was evaluated for its reactivity to recombinant virus-like particles and the detection of natural viruses from stool samples of Japanese infants and children with sporadic acute gastroenteritis compared to reverse transcription-PCR. The kit had a sensitivity of 76.3% and a specificity of 94.9%. Our results clearly indicated that the kit allows the detection of the most prevalent genotype, GII/4. In order to increase the sensitivity of the kit, the reactivity with norovirus of GII/3 and GII/6 genotypes needs to be improved.
authors.affiliation: Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan. mshoko@mail.ecc.u-tokyo.ac.jp
authors.forename: Shoko
authors.initials: S
authors.lastname: Okitsu-Negishi
authors.forename: Michio
authors.initials: M
authors.lastname: Okame
authors.forename: Yuko
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Tung Gia
authors.initials: TG
authors.lastname: Phan
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tomaru
authors.forename: Shigenori
authors.initials: S
authors.lastname: Kamijo
authors.forename: Takashi
authors.initials: T
authors.lastname: Sato
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Yagyu
authors.forename: Werner E G
authors.initials: WE
authors.lastname: Müller
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D000956:Antigens, Viral / Q000302:isolation & purification*; D017250:Caliciviridae Infections / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D005243:Feces / Q000737:chemistry*; D005759:Gastroenteritis / Q000175:diagnosis; D005838:Genotype; D006801:Humans; D007223:Infant; D029322:Norovirus / Q000235:genetics / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic