Practical aspects of uniform stable isotope labeling of higher plants for heteronuclear NMR-based metabolomics.
Analytical methods for probing plant metabolism are taking on new significance in the era of functional genomics, metabolomics, and systems biology. Nuclear magnetic resonance (NMR) is becoming a key technology in plant metabolomics. Stable isotope labeling of cultured cells and higher organisms has been especially promising in that it allows the use of advanced heteronuclear NMR methodologies through a combination of in vivo and in vitro measurements. This new approach provides much better resolution of the metabolite mixture signals in the multidimensional NMR spectra than does the conventional one-dimensional 1H-NMR previously used in plant metabolomics. In this chapter, we describe the practical aspects of two key NMR technologies: uniform stable labeling of plants and in vitro heteronuclear NMR.
Kikuchi
Jun
J
Advanced NMR Metabolomics Research Unit, RIKEN Plant Science Center, Yokohama, Japan.
Hirayama
Takashi
T
D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism*; D002460:Cell Line; D003470:Culture Media; D009587:Nitrogen Isotopes / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D031343:Perilla / Q000254:growth & development / Q000378:metabolism*; D010788:Photosynthesis; D018517:Plant Roots / Q000378:metabolism; D012987:Soil / Q000032:analysis
D002247:Carbon Isotopes; D003470:Culture Media; D009587:Nitrogen Isotopes; D012987:Soil