title: 1,4-Benzoquinone-based electrophoretic assay for glucose oxidase.
abstract: The communication demonstrates feasibility of an enzyme microassay for glucose oxidase with 1,4-benzoquinone as an acceptor of electrons. The protocol uses the plug-plug mode of electrophoretically mediated microanalysis, with nanolitre injected volumes of enzyme and reactant solutions. The reactant and product, 1,4-benzoquinone and hydroquinone, are separated during the assay by differential binding to sulfated-beta-cyclodextrin used as additive to the phosphate buffer (pH 7) and monitored at selected wavelengths in their UV spectra. The assay covers glucose oxidase concentration from 0.01 to 0.1mgml(-1). Due to the strong UV absorbance of the both reactant and product, there is no need for use of a second enzyme (peroxidase) in the present assay.
authors.affiliation: Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK.
authors.forename: Pawel L
authors.initials: PL
authors.lastname: Urban
authors.forename: David M
authors.initials: DM
authors.lastname: Goodall
authors.forename: Edmund T
authors.initials: ET
authors.lastname: Bergström
authors.forename: Neil C
authors.initials: NC
authors.lastname: Bruce
mesh_terms: D016227:Benzoquinones / Q000737:chemistry / Q000378:metabolism*; D019411:Clinical Laboratory Techniques; D019075:Electrophoresis, Capillary / Q000379:methods*; D005947:Glucose / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006873:Hydroquinones / Q000737:chemistry / Q000378:metabolism; D007202:Indicators and Reagents; D007700:Kinetics; D008832:Microchemistry; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D016227:Benzoquinones; D006873:Hydroquinones; D007202:Indicators and Reagents; C004532:quinone; D005949:Glucose Oxidase; D005947:Glucose; C031927:hydroquinone