title: Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis.
abstract: The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.
authors.affiliation: University of Konstanz, Biochemical Pharmacology, 78457 Konstanz, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Mueller
authors.forename: Sebastian
authors.initials: S
authors.lastname: Bunk
authors.forename: Isabel
authors.initials: I
authors.lastname: Diterich
authors.forename: Michael
authors.initials: M
authors.lastname: Weichel
authors.forename: Carolin
authors.initials: C
authors.lastname: Rauter
authors.forename: Dieter
authors.initials: D
authors.lastname: Hassler
authors.forename: Corinna
authors.initials: C
authors.lastname: Hermann
authors.forename: Reto
authors.initials: R
authors.lastname: Crameri
authors.forename: Thomas
authors.initials: T
authors.lastname: Hartung
mesh_terms: D000328:Adult; D025065:Borrelia burgdorferi / Q000235:genetics*; D006801:Humans; D008193:Lyme Disease / Q000175:diagnosis*; D008875:Middle Aged; D019151:Peptide Library; D012269:Ribosomal Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D019151:Peptide Library; D012269:Ribosomal Proteins; C031127:ribosomal protein L25