Tagging of avidin immobilized beads with biotinylated YAG:Ce3+ nanocrystal phosphor.
YAG:Ce3+ nanoparticles 9.5+/-1.2 nm in diameter have been synthesized from aluminium isopropoxide and acetates of yttrium and cerium in 1,4-butanediol (1,4-BD) by autoclave treatment at 300 degrees C for 2 h. After replacing 1,4-BD by ultrapure water, NH2 groups were introduced on the surface of YAG:Ce3+ nanoparticles by addition of 3-aminopropyltrimethoxysilane then biotinylation with sulfo-NHS-LC-biotin. We demonstrated that avidin immobilized beads are tagged by biotinylated YAG:Ce3+ nanoparticles by the selective avidin-biotin interaction, furnishing a green fluorescent image on excitation with blue light. This result indicates that YAG:Ce3+ nanoparticle phosphors have much potential in biological applications.
Asakura
Ryo
R
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, 223-8522, Japan.
Isobe
Tetsuhiko
T
Kurokawa
Kiyoshi
K
Aizawa
Hideki
H
Ohkubo
Michio
M
D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D002412:Cations / Q000737:chemistry; D002563:Cerium / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D012996:Solutions; D013057:Spectrum Analysis; D015019:Yttrium / Q000737:chemistry*
D002412:Cations; D003102:Colloids; D012996:Solutions; D001360:Avidin; D002563:Cerium; D015019:Yttrium; D001710:Biotin