Biotemplated nanopatterning of planar surfaces with molecular motors.
We report on the generation of nanometer-wide, non-topographical patterns of proteins on planar surfaces. In particular, we used the regular lattice of reconstituted microtubules as template structures to specifically bind and transfer kinesin-1 and nonclaret disjunctional motor proteins. The generated tracks, which comprise dense and structurally oriented arrays of functional motor proteins, proved to be highly efficient for the guiding of microtubule transporters.
Reuther
Cordula
C
Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Hajdo
Lukasz
L
Tucker
Robert
R
Kasprzak
Andrzej A
AA
Diez
Stefan
S
D003460:Crystallization / Q000379:methods*; D016547:Kinesins / Q000737:chemistry* / Q000648:ultrastructure; D046911:Macromolecular Substances; D008422:Materials Testing; D008870:Microtubules / Q000737:chemistry* / Q000648:ultrastructure; D008968:Molecular Conformation; D020409:Molecular Motor Proteins / Q000737:chemistry*; D009038:Motion; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D010316:Particle Size; D013499:Surface Properties
D046911:Macromolecular Substances; D020409:Molecular Motor Proteins; D016547:Kinesins