title: Comparative study on a homemade rapid urease test with gastric biopsy for diagnosis of Helicobacter pylori infection.
abstract: The main objective of this study was to determine whether a homemade rapid urease test was reliable when compared to histology in the diagnosis of Helicobacter pylori infection. Endoscopic antral biopsies from 15th August 2004 to 15th July 2005 were studied prospectively. Rapid urease test and histological examination were done on all specimens. Among 160 biopsies, 56 cases showed H. pylori in histological section and 59 cases were rapid urease test positive. Among 56 H. pylori infected patients, the sensitivity, specificity, positive and negative predictive values, percentage of false positive, and percentage of false negative values of rapid urease test were 96.4%, 95.2%, 91.5%, 98.0%, 4.8% and 3.5% respectively. Rapid urease test is positive in significantly higher number (p < 0.001) in histological positive cases. In conclusion our homemade rapid urease test is a test with good sensitivity and specificity for detection of H. pylori infection.
authors.affiliation: Department of Pathology, Kathmandu University Medical School, Kathmandu University Teaching Hospital Dhulikhel, Kavre, Nepal. makajuram@yahoo.com
authors.forename: Ramesh Kumar
authors.initials: RK
authors.lastname: Makaju
authors.forename: Migma Dorji
authors.initials: MD
authors.lastname: Tamang
authors.forename: Yubaraj
authors.initials: Y
authors.lastname: Sharma
authors.forename: Nastu
authors.initials: N
authors.lastname: Sharma
authors.forename: Rajendra
authors.initials: R
authors.lastname: Koju
authors.forename: Tikka Ramesh Singh
authors.initials: TR
authors.lastname: Bedi
authors.forename: Sharad
authors.initials: S
authors.lastname: Khanal
mesh_terms: D000328:Adult; D001706:Biopsy; D004724:Endoscopy; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000201:enzymology; D006801:Humans; D008297:Male; D011446:Prospective Studies; D013997:Time Factors; D014510:Urease / Q000032:analysis* / Q000378:metabolism
chemical_list: D014510:Urease