title: Cork Oak Trees (Quercus suber L.).
abstract: A transformation system for selected mature Quercus suber L. trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries the nptII and uidA genes. Evidence of stable transgene integration is obtained by polymerase chain reaction for nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos are germinated and successfully transferred to soil.
authors.affiliation: Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, C/. Catedrático Rodrigo Uría Sn, Oviedo, Spain.
authors.forename: Rubén
authors.initials: R
authors.lastname: Alvarez
authors.forename: Mariano
authors.initials: M
authors.lastname: Toribio
authors.forename: Millán
authors.initials: M
authors.lastname: Cortizo
authors.forename: Ricardo-Javier
authors.initials: RJ
authors.lastname: Ordás Fernández
mesh_terms: D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D015139:Blotting, Southern; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D003470:Culture Media; D018525:Germination; D005966:Glucuronidase / Q000032:analysis; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000196:embryology / Q000254:growth & development; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D029963:Quercus / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D012987:Soil; D014170:Transformation, Genetic; D019076:Transgenes
chemical_list: D003470:Culture Media; D012987:Soil; D005966:Glucuronidase