Characterization of DIDS-sensitive ATP accumulation in brain synaptic vesicles.
ATP is an excitatory neurotransmitter in the central and peripheral nervous system. We investigated ATP accumulation in highly purified brain synaptic vesicles (SVs). Based on the amount of ATP accumulated in SVs under the conditions used, ATP is not transported against a concentration gradient but rather appears to have a Delta mu H(+)-independent mechanism. ATP transport was inhibited by DIDS and NEM, but was not affected by Mg(2+) or by pre-incubation with nucleotides. These results suggest a unique transport mechanism that does not involve exchange with other nucleotides or protons, unlike other known neurotransmitter transport systems.
Zalk
Ran
R
Department of Life Sciences, National Institute for Biotechnology, Negev and Zlotowski Center for Neuroscience, Ben Gurion University, Life Sciences Building 40, 84105 Beer Sheva, Israel.
Shoshan-Barmatz
Varda
V
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology*; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D012756:Sheep; D013572:Synaptic Vesicles / Q000187:drug effects* / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; C033901:3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate; D000255:Adenosine Triphosphate; D005033:Ethylmaleimide; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid