[Determination of three nitroimidazole residues in royal jelly by high performance liquid chromatography-tandem mass spectrometry].
A method for analysis of trace metronidazole (MTZ), dimetridazole (DMZ) and ronidazole (RNZ) residues in royal jelly was developed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). After samples were dissolved in sodium hydroxide solution to disassociate target analytes from matrix, liquid-liquid extraction methods by ethyl acetate solvent were used. Matrix effects were minimized and good quantitation results were obtained by using highly-selective reaction monitoring (H-SRM) technology when deuterated dimetridazole (dimetridazole-D3) was selected as internal standard. Limits of detection (LODs) were 1.0 microg/kg for DMZ, 0.5 microg/kg for MTZ and RNZ (S/N > 5). Limits of quantitation (LOQs) were 2.0 microg/kg for DMZ, 1.0 microg/kg for MTZ and RNZ (S/N > 10). The linear ranges were 2.0 - 200 microg/L for all target analytes. Recoveries and relative standard deviations (RSDs) were in the ranges of 96.6% - 110.6% and 2.1% -7.4%, respectively. This method is suitable for statutory residue testing in the National Residue Surveillance Plan in China and meets the requirement for export.
Ding
Tao
T
National Honey Reference Laboratory, Animal, Plant and Food Inspection Center (APFIC) of Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, China. dingt@jsciq.gov.cn
Xu
Jinzhong
J
Shen
Chongyu
C
Jiang
Yuan
Y
Chen
Huilan
H
Wu
Bin
B
Zhao
Zengyun
Z
Li
Gonghai
G
Zhang
Jing
J
Liu
Fei
F
D002681:China; D004131:Dimetridazole / Q000032:analysis*; D005227:Fatty Acids / Q000737:chemistry*; D007491:Ipronidazole / Q000032:analysis; D057230:Limit of Detection; D008795:Metronidazole / Q000032:analysis*; D009593:Nitroimidazoles / Q000032:analysis*; D012015:Reference Standards; D012385:Ronidazole / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000099219:Royal Jelly
D004131:Dimetridazole; D005227:Fatty Acids; D007491:Ipronidazole; D008795:Metronidazole; D009593:Nitroimidazoles; D012385:Ronidazole; D000099219:Royal Jelly