title: Expression of the RERG gene is gender-dependent in hepatocellular carcinoma and regulated by histone deacetyltransferases.
abstract: Ras-related, estrogen-regulated, and growth-inhibitory gene (RERG) is a novel gene that was first reported in breast cancer. However, the functions of RERG are largely unknown in other tumor types. In this study, RERG expression was analyzed in hepatocellular carcinomas of human patients using reverse transcriptase PCR analysis. In addition, the possible regulation of RERG expression by histone deacetyltransferases (HDACs) was studied in several cell lines. Interestingly, the expression of RERG gene was increased in hepatocellular carcinoma (HCC) of male patients (57.9%) but decreased in HCC of females (87.5%) comparison with paired peri-tumoral tissues. Moreover, RERG gene expression was increased in murine hepatoma Hepa1-6 cells, human breast tumor MDA-MB-231 cells, and mouse normal fibroblast NIH3T3 cells after treated by HDAC inhibitor, trichostatin A. Our results suggest that RERG may function in a gender-dependent manner in hepatic tumorigenesis and that the expression of this gene may be regulated by an HDAC-related signaling pathway.
authors.affiliation: Laboratory of Human Genomics, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Wang
authors.forename: Wan
authors.initials: W
authors.lastname: Fang
authors.forename: Ying-Hao
authors.initials: YH
authors.lastname: Han
authors.forename: Sang-Mi
authors.initials: SM
authors.lastname: Cho
authors.forename: Jong Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Kee Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Wook Hwan
authors.initials: WH
authors.lastname: Kim
authors.forename: Jin Man
authors.initials: JM
authors.lastname: Kim
authors.forename: Moon Gi
authors.initials: MG
authors.lastname: Park
authors.forename: Dae-Yeul
authors.initials: DY
authors.lastname: Yu
authors.forename: Nam-Soon
authors.initials: NS
authors.lastname: Kim
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Lee
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D047628:Estrogen Receptor alpha / Q000032:analysis; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D006131:Growth Inhibitors / Q000235:genetics*; D022781:Hepatocytes / Q000378:metabolism; D006655:Histone Deacetylases / Q000502:physiology*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012737:Sex Factors; D015398:Signal Transduction
chemical_list: D047628:Estrogen Receptor alpha; D004967:Estrogens; D006131:Growth Inhibitors; D006655:Histone Deacetylases