Spleen-dependent turnover of CD11b peripheral blood B lymphocytes in bovine leukemia virus-infected sheep.
Lymphocyte homeostasis is determined by a critical balance between cell proliferation and death, an equilibrium which is deregulated in bovine leukemia virus (BLV)-infected sheep. We have previously shown that an excess of proliferation occurs in lymphoid tissues and that the peripheral blood population is prone to increased cell death. To further understand the mechanisms involved, we evaluated the physiological role of the spleen in this accelerated turnover. To this end, B lymphocytes were labeled in vivo using a fluorescent marker (carboxyfluorescein diacetate succinimidyl ester), and the cell kinetic parameters (proliferation and death rates) of animals before and after splenectomy were compared. We show that the enhanced cell death observed in BLV-infected sheep is abrogated after splenectomy, revealing a key role of the spleen in B-lymphocyte dynamics.
Florins
Arnaud
A
National Fund for Scientific Research, Molecular and Cellular Biology Laboratory, 13 avenue Maréchal Juin, 5030 Gembloux, Belgium.
Gillet
Nicolas
N
Asquith
Becca
B
Debacq
Christophe
C
Jean
Geneviève
G
Schwartz-Cornil
Isabelle
I
Bonneau
Michel
M
Burny
Arsène
A
Reichert
Michal
M
Kettmann
Richard
R
Willems
Luc
L
D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D039481:CD11b Antigen / Q000276:immunology*; D049109:Cell Proliferation; D017931:DNA Primers; D006800:Deltaretrovirus Infections / Q000276:immunology*; D005452:Fluoresceins; D006706:Homeostasis / Q000276:immunology*; D016130:Immunophenotyping; D007700:Kinetics; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D012756:Sheep; D013154:Spleen / Q000276:immunology*; D013156:Splenectomy; D013388:Succinimides
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D039481:CD11b Antigen; D017931:DNA Primers; D005452:Fluoresceins; D013388:Succinimides