title: A Nd:YAG laser-microperforated poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-basal membrane matrix composite film as substrate for keratinocytes.
abstract: Epithelia cultured for the treatment of ulcers, burns and for gene therapy applications require a flexible biomaterial for growth and transplantation that is adaptable to body contours. We tested several materials and found that a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) polyester provided support for keratinocytes, although adhesion to this material proved to be suboptimal. Since epithelia adhere to the mesoderm through basal membranes, we engineered a basal membrane surrogate by preparing composites of PHBHV with basal membrane matrix (BMM). To allow cell migration into injuried areas the polyester film was micromachined to insert high-density micropores through a Nd:YAG laser ablation process. These flexible composites provided firm attachment for keratinocytes from the outer root sheath of human hair allowing keratinocyte migration through micropores. Films of microperforated PHBHV-BMM may be of use for the replacement of diseased or injured skin epithelia.
authors.affiliation: Fundación Hospital de Alcorcón, Avda Villaviciosa 1, Alcorcón E-28922, Spain. fserrano@fhalcorcon.es
authors.forename: Fernando
authors.initials: F
authors.lastname: Serrano
authors.forename: Laura
authors.initials: L
authors.lastname: López-G
authors.forename: Maria
authors.initials: M
authors.lastname: Jadraque
authors.forename: Mariëlle
authors.initials: M
authors.lastname: Koper
authors.forename: Gary
authors.initials: G
authors.lastname: Ellis
authors.forename: Pilar
authors.initials: P
authors.lastname: Cano
authors.forename: Margarita
authors.initials: M
authors.lastname: Martín
authors.forename: Leoncio
authors.initials: L
authors.lastname: Garrido
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism*; D002448:Cell Adhesion; D002478:Cells, Cultured; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates