Increased gene expression of scavenger receptors and proinflammatory markers in peripheral blood mononuclear cells of hyperlipidemic males.
Interactions between peripheral blood mononuclear cells (PBMCs) and those within plaques are suggested to be pathophysiologically relevant to lipid-induced arteriosclerosis. In this study, gene expressions of scavenger receptors (CD36, CD68), LPS receptor (CD14), proinflammatory (tumor necrosis factor alpha [TNFalpha], CD40, interleukin-1 beta [IL-1beta]) and oxidative stress-related (manganese superoxide dismutase [MnSOD]) markers were analyzed in PBMCs of clinically asymptomatic males with classical proatherogenic risk factors such as smoking and/or hyperlipidemia. PBMCs were isolated from venous blood of normolipidemic non-smokers (n = 10) and smokers (n = 8), and hyperlipidemic non-smokers (n = 9) and smokers (n = 8). RNA from PBMCs was used for PCR analyses. Plasma concentrations of oxidized low-density lipoproteins (oxLDL) were measured by ELISA. The gene expressions of CD36, CD68, CD40, TNFalpha, and MnSOD were significantly higher in PBMCs of hyperlipidemics than in normolipidemics, irrespective of whether they were smoking or not. The individual expression of these genes showed significant positive correlations with each other but also with serum cholesterol or plasma oxLDL concentrations. The higher expressions of scavenger receptors, proinflammatory and oxidative stress-related genes of PBMCs are suggested to result mainly from hyperlipidemia and the accompanied increase of oxLDL concentrations.
Bonaterra
Gabriel A
GA
Department of Anatomy and Cell Biology III, University of Heidelberg, Im Neuenheimer Feld 307, 69120, Heidelberg, Germany.
Hildebrandt
Wulf
W
Bodens
Anne
A
Sauer
Roland
R
Dugi
Klaus A
KA
Deigner
Hans-Peter
HP
Turcanu
Dan
D
Heinle
Helmut
H
Dröge
Wulf
W
Metz
Jürgen
J
Kinscherf
Ralf
R
D000328:Adult; D001161:Arteriosclerosis; D015415:Biomarkers / Q000032:analysis; D001773:Blood Cells; D015870:Gene Expression; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood* / Q000235:genetics; D007249:Inflammation / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D018384:Oxidative Stress / Q000235:genetics; D051116:Receptors, Scavenger / Q000235:genetics*; D012307:Risk Factors; D015854:Up-Regulation / Q000235:genetics*
D015415:Biomarkers; D008077:Lipoproteins, LDL; D051116:Receptors, Scavenger; C099252:oxidized low density lipoprotein