title: PIAS3 induction of PRB sumoylation represses PRB transactivation by destabilizing its retention in the nucleus.
abstract: Progesterone receptor (PR) plays a critical role in cell proliferation and differentiation, and its transcriptional activity is known to be modulated by cofactor proteins. In the present study, we demonstrated that in the presence of progesterone, protein inhibitor of activated STAT-3 (PIAS3) significantly inhibited the PR transcriptional activity and the expression of progesterone-responsive genes. Reduction of endogenous PIAS3 by PIAS3 small-interfering RNA enhanced PR transactivation in a ligand-dependent manner. PIAS3 interacted with PR both in vitro and in vivo and the interaction was enhanced by progesterone. Furthermore, our findings suggested that PIAS3 strongly induced PRB sumoylation at three sites, Lys-7, Lys-388 and Lys-531. In addition, novel roles in PRB nuclear retention and transactivation were identified for these sites. Our data also suggested that PIAS3 was recruited in a largely hormone-dependent manner in response to a progesterone-responsive promoter. Finally, we demonstrated that PIAS3 inhibited the DNA-binding activity of PR and influenced its nuclear export as well as PR transactivation. Taken together, these data strongly suggested that PIAS3 played an important physiological role in PR function.
authors.affiliation: National Center of Biomedical Analysis, Beijing 100850, China.
authors.forename: Jiang-Hong
authors.initials: JH
authors.lastname: Man
authors.forename: Hui-Yan
authors.initials: HY
authors.lastname: Li
authors.forename: Pei-Jing
authors.initials: PJ
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Kun
authors.initials: K
authors.lastname: He
authors.forename: Xin
authors.initials: X
authors.lastname: Pan
authors.forename: Bing
authors.initials: B
authors.lastname: Liang
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Wei-Li
authors.initials: WL
authors.lastname: Gong
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Jin
authors.forename: Qing
authors.initials: Q
authors.lastname: Xia
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.forename: Bei-Fen
authors.initials: BF
authors.lastname: Shen
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry*; D006801:Humans; D018832:Molecular Chaperones / Q000378:metabolism*; D011374:Progesterone / Q000037:antagonists & inhibitors; D011401:Promoter Regions, Genetic; D050828:Protein Inhibitors of Activated STAT / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011980:Receptors, Progesterone / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D018832:Molecular Chaperones; C484745:PIAS3 protein, human; D050828:Protein Inhibitors of Activated STAT; D011980:Receptors, Progesterone; D025841:Small Ubiquitin-Related Modifier Proteins; C097314:progesterone receptor B; D011374:Progesterone