Effects of chlorpromazine on plasma membrane permeability and fluidity in the rat brain: a dynamic positron autoradiography and fluorescence polarization study.
Antipsychotic drugs have been widely used in psychiatry for the treatment of various mental disorders, but the underlying biochemical mechanisms of their actions still remain unclear. Although phenothiazine antipsychotic drugs have been reported to directly interact with the peripheral plasma membrane, it is not known whether these drugs actually affect plasma membrane integrity in the central nervous system. To clarify these issues, we investigated the effect of chlorpromazine (CPZ), a typical phenothiazine antipsychotic drug, on plasma membrane permeability in fresh rat brain slices using a dynamic positron autoradiography technique and [(18)F]2-fluoro-2-deoxy-D-glucose ([(18)F]FDG) as a tracer. Treatment with CPZ (> or =100 microM) resulted in the leakage of [(18)F]FDG-6-phosphate, but not [(18)F]FDG, suggesting that the [(18)F]FDG-6-phosphate efflux was not mediated by glucose transporters, but rather by plasma membrane permeabilization. The leakage of [(18)F]FDG-6-phosphate was followed by slower leakage of cytoplasmic lactate dehydrogenase, suggesting that CPZ could initially induce small membrane holes that enlarged with time. Furthermore, the addition of CPZ (> or =100 microM) caused a decrease in 1,6-diphenyl-1,3,5-hexatriene fluorescence anisotropy, which implies an increase in membrane fluidity. CPZ loading dose-dependently increased both membrane permeability and membrane fluidity, which suggested the involvement of a perturbation of membrane order in the mechanisms of membrane destabilization induced by antipsychotic drugs.
Maruoka
Nobuyuki
N
Department of Neuropsychiatry, University of Fukui, Fukui 910-1193, Japan.
Murata
Tetsuhito
T
Omata
Naoto
N
Takashima
Yasuhiro
Y
Tanii
Hideji
H
Yonekura
Yoshiharu
Y
Fujibayashi
Yasuhisa
Y
Wada
Yuji
Y
D000818:Animals; D016880:Anisotropy; D014150:Antipsychotic Agents / Q000494:pharmacology*; D001345:Autoradiography; D001921:Brain / Q000187:drug effects*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002746:Chlorpromazine / Q000494:pharmacology*; D004583:Electrons; D005454:Fluorescence Polarization; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar
D014150:Antipsychotic Agents; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D002746:Chlorpromazine