[Histoplasma capsulatum with aberrant morphology isolated in Argentina].
For the first time in Argentina, we describe a strain of Histoplasma capsulatum var. capsulatum with an aberrant morphology that was isolated from a single patient with AIDS. Mycelial phase cultures on agar Sabouraud at 25-28 degrees C showed white, glabrous, umbilicated and centrally radiated colonies. Unusual microscopic findings were the absence of typical conidia, the presence of terminal/intercalary chlamydoconidia with a diameter of 4 pm and of thickened hyphae. Fungal identification was confirmed by the detection of bands H and M species specific antigens in mycelial culture supernatants and reversion to the typical yeast phase on agar brain-heart-cysteine at 37 degrees C. The genomic DNA profile obtained by RAPD-PCR with primers 1281-1283 coincided with the predominant profile of H. capsulatum among isolates from Argentine patients.
Zuiani
M F
MF
Departamento Micología, Instituto Nacional de Enfermedades Infecciosas, ANLIS Dr. Carlos G. Malbrán, Vélez Sarsfield 563 1281, Ciudad Autónoma de Buenos Aires, Argentina.
Rivas
M C
MC
Lee
W
W
Guelfand
L
L
Davel
G
G
Canteros
C E
CE
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology*; D001118:Argentina; D004271:DNA, Fungal / Q000032:analysis; D006658:Histoplasma / Q000166:cytology / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000382:microbiology*; D006801:Humans; D019105:Random Amplified Polymorphic DNA Technique
D004271:DNA, Fungal