title: A potential molecular marker for selection against abdominal fatness in chickens.
abstract: The peroxisome proliferators-activated receptor-gamma coactivator-1alpha (PGC-1alpha) was investigated as a candidate gene for growth and fatness traits in chicken because of its prominent role in muscle fiber specialization and adipogenesis. A single nucleotide polymorphism (SNP) from G to A at position 646 of the open reading frame of chicken PGC-1alpha gene causing an Asp216Asn amino acid substitution was identified. The frequencies of alleles and genotypes were significantly different among 6 chicken breeds (P < 0.01). The White Plymouth Rock had the highest frequency (0.67) of allele G, whereas the White Leghorn had the lowest (0.18). The associations of the SNP with the growth and fatness traits were evaluated in 332 F(2) birds from an experimental cross of White Plymouth Rock x Silkies. No association was found between the SNP and growth-related traits. However, abdominal fat weight at 12 wk of age for birds with genotype GG was 34.26 and 28.71% higher than those with genotypes AA and AG, respectively (P < 0.01), indicating that the Asp216Asn polymorphism of the PGC-1alpha gene could be used as a novel potential molecular marker for selection against abdominal fatness without interfering in regular breeding for growth rate of chickens.
authors.affiliation: MOA Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100094.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Wu
authors.forename: X M
authors.initials: XM
authors.lastname: Deng
authors.forename: J Y
authors.initials: JY
authors.lastname: Li
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.forename: N
authors.initials: N
authors.lastname: Yang
mesh_terms: D050153:Abdominal Fat / Q000378:metabolism*; D019943:Amino Acid Substitution; D000818:Animals; D001823:Body Composition / Q000235:genetics*; D001947:Breeding; D002645:Chickens / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D005838:Genotype; D008297:Male; D016366:Open Reading Frames; D047495:PPAR gamma / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012641:Selection, Genetic
chemical_list: D005819:Genetic Markers; D047495:PPAR gamma