Direct determination of lycopene content in tomatoes (Lycopersicon esculentum) by attenuated total reflectance infrared spectroscopy and multivariate analysis.
Lycopene is a potent antioxidant that has been shown to play critical roles in disease prevention. Efficient assays for detection and quantification of lycopene are desirable as alternatives to time- and labor-intensive methods. Attenuated total reflectance infrared (ATR-IR) spectroscopy was used for quantification of lycopene in tomato varieties. Calibration models were developed by partial least-squares regression (PLSR) using quantitative measures of lycopene concentration from liquid chromatography as reference method. IR spectra showed a distinct marker band at 957 cm(-1) for trans Carbon-Hydrogen (CH) deformation vibration of lycopene. PLSR models predicted the lycopene content accurately and reproducibly with a correlation coefficient (sigma) of 0.96 and standard error of cross-validation <0.80 mg/100 g. ATR-IR spectroscopy allowed for rapid, simple, and accurate determination of lycopene in tomatoes with minimal sample preparation. Results suggest that the ATR-IR method is applicable for high-throughput quantitative analysis and screening for lycopene in tomatoes.
Halim
Yuwana
Y
The Ohio State University, Department of Food Science and Technology, 110 Parker Food Science and Technology Bldg, 2015 Fyffe Rd, Columbus, OH 43210, USA.
Schwartz
Steven J
SJ
Francis
David
D
Baldauf
Nathan A
NA
Rodriguez-Saona
Luis E
LE
D002338:Carotenoids / Q000032:analysis*; D002853:Chromatography, Liquid; D016018:Least-Squares Analysis; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*; D015999:Multivariate Analysis; D012016:Reference Values; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared / Q000379:methods* / Q000706:statistics & numerical data
D002338:Carotenoids; D000077276:Lycopene