title: [Determination of ginsenoside Rd and its metabolites in rat urine by LC-MS].
abstract: OBJECTIVE
  To study the metabolic pathways of ginsenoside Rd in rats.
  
  
  METHODS
  Urine samples were collected before and after 24 h of single oral administration of 150 mg and intravenous administration of 60 mg of ginsenoside Rd to six rats, separately. The samples were purified by SPE column and then were analyzed by liquid chromatography-ESI-mass spectrometry for putative metabolites.
  
  
  RESULTS
  Parent drug and its seven metabolites were identified in rat urine based on comparing total ion chromatograms of the blank with the metalolic urine as well as mass spectra. Its main metabolic pathways and possible structures are elucidated.
  
  
  CONCLUSIONS
  Oxidation, combination and deglucosylation were found to be the major metabolic pathway of ginsenoside Rd in rats.
authors.affiliation: Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine pathways, Guangzhou 510120, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Shun-jun
authors.initials: SJ
authors.lastname: Xu
authors.forename: Xing
authors.initials: X
authors.lastname: Zeng
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Shi-gui
authors.initials: SG
authors.lastname: Deng
authors.forename: Zhi-feng
authors.initials: ZF
authors.lastname: Wu
authors.forename: Run-mei
authors.initials: RM
authors.lastname: Ou
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000008:administration & dosage / Q000378:metabolism* / Q000652:urine*; D007275:Injections, Intravenous; D008297:Male; D010084:Oxidation-Reduction; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D036145:Ginsenosides; C097367:ginsenoside Rg3; C442759:ginsenoside Rb1; C055305:ginsenoside Rh2; C049863:ginsenoside Rd