Protection against nonylphenol-induced cell death by DJ-1 in cultured Japanese medaka (Oryzias latipes) cells.
The Japanese medaka (Oryzias latipes) has been used to investigate diverse aspects of toxicology, genetics and developmental biology and to monitor biological changes caused by endocrine disruptors. In this study, we analyzed a medaka homolog of human DJ-1 (meDJ-1) in cultured medaka cells into which nonylphenol (NP) was added. Like human DJ-1, meDJ-1 was found to be oxidized by treatment with H(2)O(2) and its pI was shifted to acidic points. NP was found to induce cell death with kinetics similar to that of H(2)O(2) in cultured medaka OLHE-13 cells. After OLHE-13 cells had been treated with sub-lethal concentrations of H(2)O(2) and NP, production of reactive oxygen species and oxidation of meDJ-1 were observed. meDJ-1 knockdown by short interfering RNA rendered OLHE-13 cells susceptible to H(2)O(2) and NP-induced cell death, suggesting a protective role of DJ-1 against oxidative stress-induced cell death in medaka cells. These results suggest that meDJ-1 is a suitable biomarker for oxidative stress reactions in medaka.
Li
Hong Mei
HM
Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Taira
Takahiro
T
Maita
Chinatsu
C
Ariga
Hiroyoshi
H
Iguchi-Ariga
Sanae M M
SM
D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects*; D002470:Cell Survival; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D005434:Flow Cytometry; D047908:Intracellular Signaling Peptides and Proteins / Q000494:pharmacology*; D007525:Isoelectric Focusing; D008156:Luciferases / Q000378:metabolism; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000494:pharmacology*; D009990:Oryzias / Q000502:physiology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D000071617:Protein Deglycase DJ-1; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D015415:Biomarkers; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D010636:Phenols; C025256:nonylphenol; D008156:Luciferases; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1