Detection of norovirus antigens from recombinant virus-like particles and stool samples by a commercial norovirus enzyme-linked immunosorbent assay kit.
The commercial norovirus enzyme-linked immunosorbent assay kit was evaluated for its reactivity to recombinant virus-like particles and the detection of natural viruses from stool samples of Japanese infants and children with sporadic acute gastroenteritis compared to reverse transcription-PCR. The kit had a sensitivity of 76.3% and a specificity of 94.9%. Our results clearly indicated that the kit allows the detection of the most prevalent genotype, GII/4. In order to increase the sensitivity of the kit, the reactivity with norovirus of GII/3 and GII/6 genotypes needs to be improved.
Okitsu-Negishi
Shoko
S
Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan. mshoko@mail.ecc.u-tokyo.ac.jp
Okame
Michio
M
Shimizu
Yuko
Y
Phan
Tung Gia
TG
Tomaru
Takeshi
T
Kamijo
Shigenori
S
Sato
Takashi
T
Yagyu
Fumihiro
F
Müller
Werner E G
WE
Ushijima
Hiroshi
H
D000956:Antigens, Viral / Q000302:isolation & purification*; D017250:Caliciviridae Infections / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D005243:Feces / Q000737:chemistry*; D005759:Gastroenteritis / Q000175:diagnosis; D005838:Genotype; D006801:Humans; D007223:Infant; D029322:Norovirus / Q000235:genetics / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic