title: Detection of pemphigus desmoglein 1 and desmoglein 3 autoantibodies and pemphigoid BP180 autoantibodies in saliva and comparison with serum values.
abstract: Although there is much literature on the detection of pemphigus and pemphigoid autoantibodies by enzyme-linked immunosorbent assay (ELISA) in serum, nothing is known about their presence in saliva. The aim of this study was to evaluate the salivary levels of these autoantibodies in pemphigus and pemphigoid patients. Autoantibodies against desmoglein3, desmoglein1, and BP180 were assayed, by ELISA, in serum and saliva samples of patients and healthy controls. The titres of autoantibodies against Dsg1/3 found in both serum and saliva of pemphigus patients showed a statistically significant correlation, suggesting that saliva may be a useful biological material for diagnostic purposes, in monitoring disease activity, as well as for the early detection of relapses. By contrast, the titres of autoantibodies against BP180 in the serum and saliva of bullous pemphigoid patients were not statistically related, and further study of the usefulness of the BP180 ELISA for saliva in this disease is needed. In addition, based on our results, the BP180 ELISA with a recombinant NC16a epitope failed to detect the autoantibodies against BP180 in the serum and saliva of mucous membrane pemphigoid patients.
authors.affiliation: Department of Oral Medicine and Maxillofacial Pathology, School of Dentistry, Aristotle University of Thessaloniki, Greece. lorenzinig@unisi.it
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Andreadis
authors.forename: Guido
authors.initials: G
authors.lastname: Lorenzini
authors.forename: Drakoulis
authors.initials: D
authors.lastname: Drakoulakos
authors.forename: Maria
authors.initials: M
authors.lastname: Belazi
authors.forename: Euaggelia
authors.initials: E
authors.lastname: Mihailidou
authors.forename: Georgios
authors.initials: G
authors.lastname: Velkos
authors.forename: Olga
authors.initials: O
authors.lastname: Mourellou-Tsatsou
authors.forename: Demetrios
authors.initials: D
authors.lastname: Antoniades
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000032:analysis; D001324:Autoantigens / Q000032:analysis*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D051183:Desmoglein 1 / Q000276:immunology*; D051184:Desmoglein 3 / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D024041:Non-Fibrillar Collagens; D010391:Pemphigoid, Bullous / Q000276:immunology*; D010392:Pemphigus / Q000276:immunology*; D012463:Saliva / Q000276:immunology*; D044967:Serum / Q000737:chemistry / Q000276:immunology; D000097805:Collagen Type XVII
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D051183:Desmoglein 1; D051184:Desmoglein 3; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII