Limited diagnostic capacities of two commercial assays for the detection of Leptospira immunoglobulin M antibodies in Laos.
The diagnostic utility of immunochromatographic (Leptotek) and enzyme-linked immunosorbent assay (ELISA; Panbio) tests for the detection of Leptospira immunoglobulin M antibodies was assessed in febrile adults admitted in Vientiane, Laos. Both tests demonstrated poor diagnostic accuracy using admission serum (Leptotek sensitivity of 47.3% and specificity of 75.5%: ELISA sensitivity of 60.9% and specificity of 65.6%) compared to the Leptospira "gold standard" microscopic agglutination test.
Blacksell
Stuart D
SD
Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR, and Centre for Clinical Vaccinology and Tropical Medicine, University of Oxford, UK. stuart@tropmedres.ac
Smythe
Lee
L
Phetsouvanh
Rattanaphone
R
Dohnt
Michael
M
Hartskeerl
Rudy
R
Symonds
Meegan
M
Slack
Andrew
A
Vongsouvath
Manivanh
M
Davong
Viengmone
V
Lattana
Olay
O
Phongmany
Simmaly
S
Keolouangkot
Valy
V
White
Nicholas J
NJ
Day
Nicholas P J
NP
Newton
Paul N
PN
D002845:Chromatography / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis* / Q000097:blood; D007812:Laos; D007919:Leptospira / Q000276:immunology*; D007922:Leptospirosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D012680:Sensitivity and Specificity
D007075:Immunoglobulin M