[The tk gene mutation analysis in WTK1 cell line by multiplex PCR].
OBJECTIVE
To explore the tk gene mutation spectrum in spontaneous and induced WTK1 mutants.


METHODS
After exposure to methyl methanesulfonate (MMS), mitomycin (MMC) or sodiam azide (NaN3), spontaneously-arising and induced mutants of WTK1 cell line were selected. The spectrum and hotspot of tk gene mutation were analyzed by multiplex PCR.


RESULTS
Three chemicals used in this study induced tk mutation frequency (MF) to increase significantly in a dose-dependent manner; most of the analyzed mutants had lost exon 4 and exon 5-7.


CONCLUSIONS
Three chemicals have mutagenic effect on WTK1 cell line, and obvious hotspot exists in tk gene mutation.
Wang
Yi-jing
YJ
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Shuai
Pei-qiang
PQ
Zhang
Li-shi
LS
D002478:Cells, Cultured; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000201:enzymology*; D008741:Methyl Methanesulfonate / Q000633:toxicity; D016685:Mitomycin / Q000633:toxicity; D009152:Mutagenicity Tests; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D019810:Sodium Azide / Q000633:toxicity; D013937:Thymidine Kinase / Q000235:genetics*
D016685:Mitomycin; D019810:Sodium Azide; D008741:Methyl Methanesulfonate; D013937:Thymidine Kinase