Immune modulation by probiotic strains: quantification of phagocytosis of Aeromonas salmonicida by leukocytes isolated from gut of rainbow trout (Oncorhynchus mykiss) using a radiolabelling assay.
A method is described for the rapid and sensitive assay of phagocytosis that utilizes radioactively labelled bacteria. With this method, we observed that phagocytosis of heat-inactivated Aeromonas salmonicida by leukocytes isolated from gut of rainbow trout fed with different viable probiotics (Lactococcus lactis subsp. lactis, Lactobacillus sakei, and Leuconostoc mesenteroides) was significantly higher (P<0.05) after 2 weeks of probiotic-feeding than the control group. However, only phagocytosis of live A. salmonicida by the leukocytes isolated from gut of rainbow trout fed with L. lactis subsp. lactis was significantly higher (P<0.05) than the control group.
Balcázar
José Luis
JL
Laboratory of Fish Pathology, University of Zaragoza, Zaragoza 50013, Spain. balcazar@unizar.es
Vendrell
Daniel
D
de Blas
Ignacio
I
Ruiz-Zarzuela
Imanol
I
Gironés
Olivia
O
Muzquiz
José Luis
JL
D048409:Aeromonas salmonicida / Q000502:physiology; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D007778:Lactobacillus / Q000502:physiology; D013294:Lactococcus lactis / Q000502:physiology; D007934:Leuconostoc / Q000502:physiology; D007962:Leukocytes / Q000276:immunology; D017686:Oncorhynchus mykiss / Q000276:immunology*; D010587:Phagocytosis / Q000502:physiology*; D019936:Probiotics / Q000494:pharmacology*; D013270:Stomach / Q000166:cytology; D014316:Tritium
D014316:Tritium