Simple and environmental friendly procedure for the gas chromatographic-mass spectrometric determination of caffeine in beverages.
A simple, quick and organic solvent saving procedure has been developed for the GC/MS determination of caffeine in beverages. The procedure involves the mixing of 25 microL sample with 1 mL ethyl acetate, and a following simple desiccation procedure in a 1.5 mL autosampler vial. A linear calibration curve was generated with caffeine concentration ranging from 0.005 mg/L to 30.0mg/L. The procedure developed provides a 0.001 mg/L detection limit of caffeine in the final solution by injection of 1 microL solution and the relative standard deviation (RSD) was less than 2% for independent measurement. The total amount of organic solvent used for individual detection is 1 mL of nontoxic ethyl acetate. The developed method was repeatable and could be applied to determine trace amounts of caffeine in popular commercial beverages.
Zou
Jiankai
J
Department of Chemistry, Zhejiang University, Zheda Road 38#, Hangzhou 310027, China. zoujiankai@yahoo.com.cn
Li
Na
N
D001628:Beverages / Q000032:analysis*; D002110:Caffeine / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results
D002110:Caffeine