[Anticancer therapeutic effect of SEA-linked and membrane-bound HSP70-expressed intestine-carcinoma vaccine].
OBJECTIVE
To develop a novel dual-modified vaccine, the superantigen-linked intestine-carcinoma cells expressing membrane-bound heat shock protein 70 (HSP70), and further examine its anticancer therapeutic effect.


METHODS
The pre-established intestine carcinoma CT26 line expressing membrane-bound heat shock protein 70 (HSP70) was amplified and incubated with superantigen fusion protein, staphylococcal enterotoxin A (SEA) fused with transmembrane sequence (SEA-TM), thereby the dual-modified vaccine was prepared after inactivation. The anticancer efficacy of the vaccine was examined.


RESULTS
The laser confocal microscopy and flow cytometry showed that there co-existed much HSP70 and SEA on the vaccine membrane surface. Both of the single-modified vaccines, the SEA-linked vaccine and membrane-bound-HSP70-expressing one, displayed marked tumor suppression, a prolonged survival period, augmented lymphocyte proliferation and higher NK and CTL activity in the vaccinated mice when compared with its counterpart. Furthermore, the dually modified vaccine induced lymphocyte proliferation most intensively, generated the highest NK and CTL activity as well as the strongest tumor rejection in the vaccinated mice. The survival period of the mice was further prolonged.


CONCLUSIONS
A new vaccine, SEA-linked and membrane-bound-HSP70-expressing intestine-carcinoma cells can induce more potent anticancer immunity and produce better therapeutic efficacy.
Huang
Chang-xin
CX
Department of Colorectal Surgery, The Third People's Hospital, Hangzhou 310009, China. hcx12@tom.com
Yu
Hai
H
Yang
Guan-gen
GG
Wang
Qing-qing
QQ
Li
Da
D
Ma
Wen-xue
WX
Shen
Fen-ping
FP
D000818:Animals; D019496:Cancer Vaccines / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D004768:Enterotoxins / Q000276:immunology*; D015870:Gene Expression; D005822:Genetic Vectors; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018089:Superantigens / Q000276:immunology; D014162:Transfection
D019496:Cancer Vaccines; D004768:Enterotoxins; D018840:HSP70 Heat-Shock Proteins; D018089:Superantigens; C026066:enterotoxin A, Staphylococcal