The chemical modification of liposome surfaces via a copper-mediated [3 + 2] azide-alkyne cycloaddition monitored by a colorimetric assay.
A generic method for the efficient in-situ modification of liposomes is described based on "click" chemistry, and a simple colorimetric assay is developed for monitoring the reaction.
Cavalli
Silvia
S
Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Tipton
Alicia R
AR
Overhand
Mark
M
Kros
Alexander
A
D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D003124:Colorimetry; D003300:Copper / Q000737:chemistry*; D003500:Cyclization; D031541:Fluorescence Resonance Energy Transfer; D008081:Liposomes / Q000737:chemistry; D015394:Molecular Structure; D012996:Solutions; D013499:Surface Properties
D000480:Alkynes; D001386:Azides; D008081:Liposomes; D012996:Solutions; D003300:Copper