Emergence of ofloxacin resistance in Mycobacterium tuberculosis clinical isolates from China as determined by gyrA mutation analysis using denaturing high-pressure liquid chromatography and DNA sequencing.
A high rate of double point mutations in gyrA (56% of 87 ofloxacin-resistant Mycobacterium tuberculosis clinical isolates) indicates the emergence of fluoroquinolone resistance. This is the first report to describe denaturing high-pressure liquid chromatography analysis of mutations in gyrA of M. tuberculosis in a large number of clinical isolates.
Shi
Ruiru
R
Mycobacterial Reference Center, The Research Institute of Tuberculosis, 3-1-24 Matsuyama, Kiyose, Tokyo 204-0022, Japan.
Zhang
Jianyuan
J
Li
Chuanyou
C
Kazumi
Yuko
Y
Sugawara
Isamu
I
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002681:China; D002851:Chromatography, High Pressure Liquid; D027081:DNA Gyrase / Q000235:genetics*; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D020125:Mutation, Missense; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D015242:Ofloxacin / Q000494:pharmacology*; D017354:Point Mutation; D017422:Sequence Analysis, DNA; D014376:Tuberculosis / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015242:Ofloxacin; D027081:DNA Gyrase