Evaluation of four different fecal tests for determination of cure after Helicobacter pylori treatment.
BACKGROUND
Data evaluating the monoclonal tests for determination of cure after Helicobacter pylori treatment are scarce.


OBJECTIVE
This study was aimed to evaluate the usefulness of 4 stool tests-2 new RAPID monoclonal immunochromatographic tests (RAPID Hp StAR, DakoCytomation, Cambridge, UK and ImmunoCard STAT! HpSA, Meridian Diagnostics, Cincinnati, OH) a monoclonal EIA test (Amplified IDEIA Hp StAR, DakoCytomation, Cambridge, UK), and a polyclonal EIA test (Premier Platinum HpSA, Meridian Diagnostics, Cincinnati, OH)-to confirm cure of H. pylori infection after eradication treatment.


METHODS
Ninety-seven patients who underwent eradication treatment were included. Cure of H. pylori infection was determined using 2 consecutive reference tests. Fecal tests were performed according to the specifications of the manufacturer. Sensitivity, specificity, and positive and negative predictive values were calculated.


RESULTS
After H. pylori eradication, the RAPID Hp StAR test has a sensitivity of 73% for detecting persistent infection, a specificity of 96% to 98%, a positive predictive value of 73% to 80% and a negative predictive value of 96%. For ImmunoCard STAT! HpSA the corresponding values were 91%, 97%, 77%, and 99%, for Amplified IDEIA Hp StAR 73%, 97%, 73%, and 97%, and for Premier Platinum HpSA 91%, 79%, 35%, and 98%.


CONCLUSIONS
All tests except Premier Platinum HpSA were highly accurate confirming eradication after treatment.
Quesada
Mariela
M
Unitat de Malalties Digestives, Hospital de Sabadell, Institut Universitari Parc Taulí, Barcelona, Spain.
Calvet
Xavier
X
Dosal
Angelina
A
Calvet
Victoria
V
Sanfeliu
Isabel
I
Ribera
Laura
L
Choat
Theresa
T
Fallowfield
Barbara
B
Montserrat
Antònia
A
Puig
Valentí
V
Segura
Ferran
F
D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology*; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000188:drug therapy*; D016480:Helicobacter pylori; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D016896:Treatment Outcome
D011933:Reagent Kits, Diagnostic