title: Practical aspects of uniform stable isotope labeling of higher plants for heteronuclear NMR-based metabolomics.
abstract: Analytical methods for probing plant metabolism are taking on new significance in the era of functional genomics, metabolomics, and systems biology. Nuclear magnetic resonance (NMR) is becoming a key technology in plant metabolomics. Stable isotope labeling of cultured cells and higher organisms has been especially promising in that it allows the use of advanced heteronuclear NMR methodologies through a combination of in vivo and in vitro measurements. This new approach provides much better resolution of the metabolite mixture signals in the multidimensional NMR spectra than does the conventional one-dimensional 1H-NMR previously used in plant metabolomics. In this chapter, we describe the practical aspects of two key NMR technologies: uniform stable labeling of plants and in vitro heteronuclear NMR.
authors.affiliation: Advanced NMR Metabolomics Research Unit, RIKEN Plant Science Center, Yokohama, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Kikuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirayama
mesh_terms: D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism*; D002460:Cell Line; D003470:Culture Media; D009587:Nitrogen Isotopes / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D031343:Perilla / Q000254:growth & development / Q000378:metabolism*; D010788:Photosynthesis; D018517:Plant Roots / Q000378:metabolism; D012987:Soil / Q000032:analysis
chemical_list: D002247:Carbon Isotopes; D003470:Culture Media; D009587:Nitrogen Isotopes; D012987:Soil