Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis.
Monocyte adhesion to vascular endothelium is an initial step in atherogenesis. To quantify this, we incubated monocytes with cultured endothelial cells, and quantified the adhered live monocytes using a colorimetric assay. Endothelium activated with lipopolysaccharide attracted monocytes in a dose-dependent manner and the adhesion was attenuated with post-treatments with L-ascorbic acid (53%), alpha- (40%) and gamma-tocopherol (39%), resveratrol (39%), and Lithospermum erythrorhizon root extract (45%). This non-radioactive, colorimetric assay may be useful for screening anti-atherogenic compounds in early atherogenesis.
Jun
Hee Jin
HJ
Division of Food Science, College of Life Science and Biotechnology, Institute of Biomedical Sciences and Safety, Korea University, Seoul, 136-713, Korea.
Chung
Mi Ja
MJ
Kim
Sang Yeon
SY
Lee
Ho Joung
HJ
Lee
Sung Joon
SJ
D050197:Atherosclerosis; D002448:Cell Adhesion; D002478:Cells, Cultured; D018920:Coculture Techniques; D003124:Colorimetry / Q000295:instrumentation* / Q000379:methods; D019295:Computational Biology / Q000295:instrumentation* / Q000379:methods; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism; D029684:Lithospermum / Q000378:metabolism; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D018517:Plant Roots; D024502:alpha-Tocopherol / Q000737:chemistry; D024504:gamma-Tocopherol / Q000737:chemistry
D008070:Lipopolysaccharides; D024504:gamma-Tocopherol; D024502:alpha-Tocopherol