[Analysis of the non-Hodgkin lymphoma's clinic and blood marrow cytogenetics].
OBJECTIVE
To analyze the relationship among cytogenetics, hematopathology, morphology, clinic tumor index and prognosis of the non-Hodgkin lymphoma(NHL) patients.


METHODS
Cytogenetics analysis of bone marrow cells was performed by direct method and 24 h culture method in 20 cases of NHL. Serous P53 protein was detected in all patients.


RESULTS
Out of the twenty NHL patients, eight had bone marrow infiltration, and nine were found with abnormal karyotype: three were chromosome number abnormalities, one was t(14,18)(q32;q21), one was 14q+, two were t(8;14)(q24;q32), two were t(8;14)(q24;q32) with additional changes of chromosome. The serous P53 protein of patients with abnormal karyotype was significantly higher than that with normal karyotype.


CONCLUSIONS
t(8;14) (q24;q32) is the most classic translocation in NHL, The patients with this karyotype have worse prognosis. The increase of mutant serous P53 relates to abnormal karyotype, and the patients with high mutant P53 level in serum also have worse prognosis.
Zhou
Min
M
Department of Hematology, the Affiliated Changzhou Second People's Hospital, Nanjing Medical University, Changzhou, Jiangsu, 213003 PR China. zhoumin@medmail.com.cn
Cen
Ling
L
Xue
Yong-quan
YQ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000378:metabolism*; D002648:Child; D002869:Chromosome Aberrations; D003582:Cytogenetics / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D014178:Translocation, Genetic; D016159:Tumor Suppressor Protein p53 / Q000097:blood
D016159:Tumor Suppressor Protein p53