title: Supplementation with a complex of active nutrients improved dermal and epidermal characteristics in skin equivalents generated from fibroblasts from young or aged donors.
abstract: Cultured skin equivalent (SE, Mimeskin) was generated by co-culturing skin fibroblasts and keratinocytes on a collagen-glycosaminoglycan-chitosan dermal substrate. In order to examine donor age effect, fibroblasts from 19- (young) or 49- (aged) year-old females were used. Culture medium was supplemented with nutrients complex containing soy extract, tomato extract, grape seed extract, white tea extract, sodium ascorbate, tocopherol acetate, zinc gluconate and BioMarine complex. Epidermal and dermal structure and composition were examined after 42 and 60 days of culture. In untreated samples, SE generated from young fibroblasts was superior to SE from aged fibroblasts in all characteristics. Those include number and regularity of keratinocyte layers, number of keratinocytes expressing proliferation marker Ki67, content of collagen type I, fibrillin-1, elastin, and SE lifespan. Effects of nutritional supplementation were observed in SE from both young and aged fibroblasts, however, those effects were more pronounced in SE from aged fibroblasts. In epidermis, the treatment increased number of keratinocyte layers and delayed epidermal senescence. The number of cells expressing Ki67 was nine folds higher than those of controls, and was similar to that of young cell SE. In dermis, the treatment increased mRNA synthesis of collagen I, fibrillin-1 and elastin. In conclusion, skin cell donor age had major important effect on formation of reconstructed SE. Imperfections in epidermal and dermal structure and composition as well as life span in SE from aged cells can be improved by supplementation with active nutrients.
authors.affiliation: Banque de Tissus et Cellules, Hôpital Edouard Herriot, Hospices Civils de Lyon, Lyon, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Lacroix
authors.forename: Charbel
authors.initials: C
authors.lastname: Bouez
authors.forename: Sandrine
authors.initials: S
authors.lastname: Vidal
authors.forename: Valérie
authors.initials: V
authors.lastname: Cenizo
authors.forename: Corinne
authors.initials: C
authors.lastname: Reymermier
authors.forename: Virginie
authors.initials: V
authors.lastname: Justin
authors.forename: Jana
authors.initials: J
authors.lastname: Vicanová
authors.forename: Odile
authors.initials: O
authors.lastname: Damour
mesh_terms: D000328:Adult; D000375:Aging / Q000502:physiology*; D021522:Bioartificial Organs; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D020405:Dermis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004549:Elastin / Q000235:genetics / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D000071838:Fibrillin-1; D000071837:Fibrillins; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006025:Glycosaminoglycans / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects; D017768:Skin, Artificial; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D054392:Adipokines
chemical_list: D024042:Collagen Type I; D004549:Elastin; D000071838:Fibrillin-1; D000071837:Fibrillins; D006025:Glycosaminoglycans; D008840:Microfilament Proteins; D011506:Proteins; D012333:RNA, Messenger; C000605722:FBN1 protein, human; D054392:Adipokines; C070172:Imedeen