Expression of the RERG gene is gender-dependent in hepatocellular carcinoma and regulated by histone deacetyltransferases.
Ras-related, estrogen-regulated, and growth-inhibitory gene (RERG) is a novel gene that was first reported in breast cancer. However, the functions of RERG are largely unknown in other tumor types. In this study, RERG expression was analyzed in hepatocellular carcinomas of human patients using reverse transcriptase PCR analysis. In addition, the possible regulation of RERG expression by histone deacetyltransferases (HDACs) was studied in several cell lines. Interestingly, the expression of RERG gene was increased in hepatocellular carcinoma (HCC) of male patients (57.9%) but decreased in HCC of females (87.5%) comparison with paired peri-tumoral tissues. Moreover, RERG gene expression was increased in murine hepatoma Hepa1-6 cells, human breast tumor MDA-MB-231 cells, and mouse normal fibroblast NIH3T3 cells after treated by HDAC inhibitor, trichostatin A. Our results suggest that RERG may function in a gender-dependent manner in hepatic tumorigenesis and that the expression of this gene may be regulated by an HDAC-related signaling pathway.
Wang
Ai-Guo
AG
Laboratory of Human Genomics, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.
Fang
Wan
W
Han
Ying-Hao
YH
Cho
Sang-Mi
SM
Choi
Jong Young
JY
Lee
Kee Ho
KH
Kim
Wook Hwan
WH
Kim
Jin Man
JM
Park
Moon Gi
MG
Yu
Dae-Yeul
DY
Kim
Nam-Soon
NS
Lee
Dong-Seok
DS
D000818:Animals; D049109:Cell Proliferation; D047628:Estrogen Receptor alpha / Q000032:analysis; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D006131:Growth Inhibitors / Q000235:genetics*; D022781:Hepatocytes / Q000378:metabolism; D006655:Histone Deacetylases / Q000502:physiology*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012737:Sex Factors; D015398:Signal Transduction
D047628:Estrogen Receptor alpha; D004967:Estrogens; D006131:Growth Inhibitors; D006655:Histone Deacetylases