Replisome localization in vegetative and aerial hyphae of Streptomyces coelicolor.
Using a functional fusion of DnaN to enhanced green fluorescent protein, we examined the subcellular localization of the replisome machinery in the vegetative mycelium and aerial mycelium of the multinucleoid organism Streptomyces coelicolor. Chromosome replication took place in many compartments of both types of hypha, with the apical compartments of the aerial mycelium exhibiting the highest replication activity. Within a single compartment, the number of "current" ongoing DNA replications was lower than the expected chromosome number, and the appearance of fluorescent foci was often heterogeneous, indicating that this process is asynchronous within compartments and that only selected chromosomes undergo replication.
Ruban-Ośmiałowska
Beata
B
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Weigla 12, 53-114, Wrocław, Poland. zakrzew@iitd.pan.wroc.pl
Jakimowicz
Dagmara
D
Smulczyk-Krawczyszyn
Aleksandra
A
Chater
Keith F
KF
Zakrzewska-Czerwińska
Jolanta
J
D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000032:analysis / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D046911:Macromolecular Substances / Q000032:analysis*; D008856:Microscopy, Fluorescence; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D048372:Streptomyces coelicolor / Q000166:cytology / Q000502:physiology*
D001426:Bacterial Proteins; D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D004259:DNA-Directed DNA Polymerase; C083980:dnaN protein, Bacteria