[Induction of chromosome aberrations and micronuclei in human peripheral blood lymphocytes at low dose of radiation].
The chromosome damage induced by the doses of y-irradiation 6)Co in peripheral blood lymphocytes was studied using different cytogenetic assays. Isolated lymphocytes were exposed to 0.01-1.0 Gy, stimulated by PHA, and analysed for chromosome aberrations at 48 h postirradiation by metaphase method, at 49 h--by the anaphase method, at 58 h by micronucleus assay with cytochalasin B and, additionally, micronuclei were counted at 48 h on the slides prepared for the metaphase analysis without cytochalasin B. Despite of the quantitative differences in the amount of chromosome damage revealed by different methods all of them demonstrated complex nonlinear dose dependence of the frequency of aberrant cells and aberrations. At the dose range from 0.01 Gy to 0.05-0.07 Gy the cells had the highest radiosensitivity mainly due to chromatid-type aberration induction. With dose increasing the frequency of the aberrant cells and aberrations decreased significantly (in some cases to the control level). At the doses up to 0.5-0.7 Gy the dose-effect curves have become linear with the decreased slope compare to initial one (by factor of 5 to 10 for different criteria) reflecting the higher radioresistance of cells. These data confirm the idea that the direct linear extrapolation of high dose effect to low dose range--the procedure routinelly used to estimate genetic risk of low dose irradiation--cannot be effective and may lead to underestimation of chromosome damage produced by low radiation doses. Preferences and disadvantages of used cytogenetic assays and possible mechanisms of low ionising radiation doses action were discussed.
Shmakova
N L
NL
Nasonova
E A
EA
Krasavin
E A
EA
Komova
O V
OV
Mel'nikova
L A
LA
Fadeeva
T A
TA
D002478:Cells, Cultured; D002869:Chromosome Aberrations; D019457:Chromosome Breakage; D002877:Chromosomes, Human / Q000528:radiation effects*; D003037:Cobalt Radioisotopes / Q000633:toxicity; D003571:Cytochalasin B / Q000494:pharmacology; D020732:Cytogenetic Analysis; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests
D003037:Cobalt Radioisotopes; D003571:Cytochalasin B