Distribution of DJ-1, Parkinson's disease-related protein PARK7, and its alteration in 6-hydroxydopamine-treated hemiparkinsonian rat brain.
DJ-1 has multiple functions and its dysfunction may be linked to the onset of familial Parkinson's disease PARK7. However, the function and distribution of DJ-1 is unclear. In this study, we determined DJ-1 distribution and change after intranigral injection of 6-hydroxydopamine (6-OHDA). Although distribution of DJ-1 immunoreactivity was not changed in cerebral cortex and striatum, 6-OHDA caused increase of DJ-1 in the particulate fraction and decrease in the cytosolic fraction in substantia nigra. At that time, DJ-1 shifted to acid forms. These results suggest that distributional changes, translocation, and acidic shift of DJ-1 may be compensatory responses to protect against 6-OHDA-induced oxidative stress.
Yanagida
Takashi
T
Department of Neurobiology and 21st Century COE Program, Kyoto Pharmaceutical University, Japan.
Takata
Kazuyuki
K
Inden
Masatoshi
M
Kitamura
Yoshihisa
Y
Taniguchi
Takashi
T
Yoshimoto
Kanji
K
Taira
Takahiro
T
Ariga
Hiroyoshi
H
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D008636:Mesencephalon / Q000166:cytology; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D016627:Oxidopamine; D010300:Parkinson Disease / Q000378:metabolism*; D000071617:Protein Deglycase DJ-1; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D008869:Microtubule-Associated Proteins; D016627:Oxidopamine; C404267:PARK7 protein, rat; D000071617:Protein Deglycase DJ-1