Early phase of reperfusion of human kidney allograft does not affect an erythrocyte anti-oxidative system.
BACKGROUND
Generation of reactive oxygen specimens is the basic mechanism leading to ischaemia/reperfusion injury of the kidney graft. Oxygen burst is a trigger for sophisticated biochemical changes leading to generation of oxygenated lipids and changes in microcirculation, which recruit recipient's neutrophils and contribute to delayed graft function. It has been shown that the free radicals generation correlates with the activity of anti-oxidative system. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione (GSH) are involved in protection against free radicals.


OBJECTIVE
To examine the activity of erythrocyte anti-oxidative system during reperfusion of the transplanted kidney allograft.


METHODS
The study included 40 renal transplant recipients. Blood was taken from the iliac vein before transplantation and from the graft's renal vein immediately, as well as 2 and 4 min after total reperfusion. The authors assessed the process of reperfusion using ThermaCAM SC500 termovision camera. Spectrophotometric methods were used to measure superoxide dismutase, glutathione peroxidase and catalase activity as well as glutathione concentrations in erythrocytes.


RESULTS
There were no statistically significant differences in the activities of superoxide dismutase, catalase and glutathione peroxidase as well as glutathione concentrations during the first 4 min after total graft reperfusion. Nevertheless, there was a positive correlation between the activity of superoxide dismutase and glutathione peroxidase.


CONCLUSIONS
The results suggest that the erythrocyte anti-oxidative system is stable during the early phase after reperfusion. An association between some anti-oxidative enzymes was noted.
Domański
Leszek
L
Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczeci, Poland. domanle@sci.pam.szczecin.pl
Dołgowska
Barbara
B
Safranow
Krzysztof
K
Myślak
Marek
M
Rózański
Jacek
J
Romanowski
Maciej
M
Ostrowski
Marek
M
Domański
Maciej
M
Pawlik
Andrzej
A
Ciechanowski
Kazimierz
K
D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007084:Iliac Vein; D007668:Kidney / Q000378:metabolism / Q000601:surgery; D007676:Kidney Failure, Chronic / Q000378:metabolism / Q000601:surgery*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D015427:Reperfusion Injury / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014184:Transplantation, Homologous
D000975:Antioxidants; D005609:Free Radicals; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase