Stimulative effects of Ulmus davidiana Planch (Ulmaceae) on osteoblastic MC3T3-E1 cells.
Ulmus davidiana Planch (Ulmaceae) has long been known to have anti-inflammatory and protective effects on damaged tissue, inflammation and bone among other functions. To treat rheumatoid arthritis (RA), a herbal medicine, Ulmus davidiana Planch (Ulmaceae) extract (UD) is being used in traditional oriental medicine. The effect of UD on the proliferation and osteoblastic differentiation in non-transformed osteoblastic cells (MC3T3-E1) was studied. UD dose-dependently increased DNA synthesis (significant at 5-20 microg/ml). UD increased alkaline phosphatase (ALP) activity and prolyl hydroxylase activity of MC3T3-E1 cells (5-20 microg/ml). Antiestrogen tamoxifen eliminated the stimulation of proliferation and ALP activity of MC3T3-E1, which was induced by UD. UD at concentrations ranged from 30 to 100 microg/ml inhibited prostaglandin E2 production in MC3T3-E1. These results indicate that UD directly stimulates cell proliferation and differentiation of osteoblasts. These results also suggest and UD is effective for bone anti-resorptive action in bone cells.
Suh
Seok-Jong
SJ
Molecular and Cellular Glycobiology Unit, Department of Biological Science, Sungkyunkwan University, Suwon City, Kyunggi-Do 440-764, Republic of Korea.
Yun
Woo-Sik
WS
Kim
Kap-Sung
KS
Jin
Un-Ho
UH
Kim
June-Ki
JK
Kim
Myung-Sunny
MS
Kwon
Dae Young
DY
Kim
Cheorl-Ho
CH
D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015232:Dinoprostone / Q000378:metabolism; D004965:Estrogen Antagonists / Q000494:pharmacology; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology; D034403:Ulmus / Q000737:chemistry*
D004965:Estrogen Antagonists; D010936:Plant Extracts; D013629:Tamoxifen; D011393:Procollagen-Proline Dioxygenase; D000469:Alkaline Phosphatase; D015232:Dinoprostone