title: In vitro evaluation of the prebiotic activity of a pectic oligosaccharide-rich extract enzymatically derived from bergamot peel.
abstract: The prebiotic effect of a pectic oligosaccharide-rich extract enzymatically derived from bergamot peel was studied using pure and mixed cultures of human faecal bacteria. This was compared to the prebiotic effect of fructo-oligosaccharides (FOS). Individual species of bifidobacteria and lactobacilli responded positively to the addition of the bergamot extract, which contained oligosaccharides in the range of three to seven. Fermentation studies were also carried out in controlled pH batch mixed human faecal cultures and changes in gut bacterial groups were monitored over 24 h by fluorescent in situ hybridisation, a culture-independent microbial assessment. Addition of the bergamot oligosaccharides (BOS) resulted in a high increase in the number of bifidobacteria and lactobacilli, whereas the clostridial population decreased. A prebiotic index (PI) was calculated for both FOS and BOS after 10 and 24 h incubation. Generally, higher PI scores were obtained after 10 h incubation, with BOS showing a greater value (6.90) than FOS (6.12).
authors.affiliation: Sustainability of the Food Chain Exploitation Platform, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK.
authors.forename: G
authors.initials: G
authors.lastname: Mandalari
authors.forename: C
authors.initials: C
authors.lastname: Nueno Palop
authors.forename: K
authors.initials: K
authors.lastname: Tuohy
authors.forename: G R
authors.initials: GR
authors.lastname: Gibson
authors.forename: R N
authors.initials: RN
authors.lastname: Bennett
authors.forename: K W
authors.initials: KW
authors.lastname: Waldron
authors.forename: G
authors.initials: G
authors.lastname: Bisignano
authors.forename: A
authors.initials: A
authors.lastname: Narbad
authors.forename: C B
authors.initials: CB
authors.lastname: Faulds
mesh_terms: D001419:Bacteria / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017404:In Situ Hybridization, Fluorescence; D009844:Oligosaccharides / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D010936:Plant Extracts; C116580:fructooligosaccharide