title: Optimisation of biochemical condition and substrates in vitro for tissue engineering of ligament.
abstract: In this work, we analysed the effect of growth factors on in vitro cell proliferation and collagens synthesis by fibroblasts cultured for 72 h on different substrates (silicon sheet with or without 1% gelatin, and glass as control surface) for ligament tissue engineering. A human fibroblast cell line (CRL-2703) was used. The synthesis of type I and type III collagens were evaluated qualitatively and quantitatively by RT-PCR and confocal microscopy, respectively. Cell proliferation was evaluated by two methods: (1) MTT assay (2) cell cycle analysis. It was found that PDGF-AB stimulate the proliferation of fibroblast cultured on gelatin coated silicon sheet in dose dependant manner with a maximum effect at 10 ng ml(-1). The exogenous TGF-beta1 induced the expression of type I and type III collagens in a dose and substrate-dependant manner. We deduce from this work that biochemical conditions and substrates have an important impact for optimisation of the tissue neo synthesis.
authors.affiliation: Cell and Tissue Engineering and Mechanics group, LEMTA-UMR CNRS 7563, IFR 111-Bioengineering, UHP Nancy, Vandoeuvre-lès-Nancy, 54500, France. shalaw.fawzi@medecine.uhp-nancy.fr
authors.forename: Shalaw
authors.initials: S
authors.lastname: Fawzi-Grancher
authors.forename: Natalia
authors.initials: N
authors.lastname: De Isla
authors.forename: Gilbert
authors.initials: G
authors.lastname: Faure
authors.forename: Jean François
authors.initials: JF
authors.lastname: Stoltz
authors.forename: Sylvaine
authors.initials: S
authors.lastname: Muller
mesh_terms: D001671:Biochemistry / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage*; D008022:Ligaments / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins