title: [Determination of three nitroimidazole residues in royal jelly by high performance liquid chromatography-tandem mass spectrometry].
abstract: A method for analysis of trace metronidazole (MTZ), dimetridazole (DMZ) and ronidazole (RNZ) residues in royal jelly was developed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). After samples were dissolved in sodium hydroxide solution to disassociate target analytes from matrix, liquid-liquid extraction methods by ethyl acetate solvent were used. Matrix effects were minimized and good quantitation results were obtained by using highly-selective reaction monitoring (H-SRM) technology when deuterated dimetridazole (dimetridazole-D3) was selected as internal standard. Limits of detection (LODs) were 1.0 microg/kg for DMZ, 0.5 microg/kg for MTZ and RNZ (S/N > 5). Limits of quantitation (LOQs) were 2.0 microg/kg for DMZ, 1.0 microg/kg for MTZ and RNZ (S/N > 10). The linear ranges were 2.0 - 200 microg/L for all target analytes. Recoveries and relative standard deviations (RSDs) were in the ranges of 96.6% - 110.6% and 2.1% -7.4%, respectively. This method is suitable for statutory residue testing in the National Residue Surveillance Plan in China and meets the requirement for export.
authors.affiliation: National Honey Reference Laboratory, Animal, Plant and Food Inspection Center (APFIC) of Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, China. dingt@jsciq.gov.cn
authors.forename: Tao
authors.initials: T
authors.lastname: Ding
authors.forename: Jinzhong
authors.initials: J
authors.lastname: Xu
authors.forename: Chongyu
authors.initials: C
authors.lastname: Shen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Huilan
authors.initials: H
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Zengyun
authors.initials: Z
authors.lastname: Zhao
authors.forename: Gonghai
authors.initials: G
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
mesh_terms: D002681:China; D004131:Dimetridazole / Q000032:analysis*; D005227:Fatty Acids / Q000737:chemistry*; D007491:Ipronidazole / Q000032:analysis; D057230:Limit of Detection; D008795:Metronidazole / Q000032:analysis*; D009593:Nitroimidazoles / Q000032:analysis*; D012015:Reference Standards; D012385:Ronidazole / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000099219:Royal Jelly
chemical_list: D004131:Dimetridazole; D005227:Fatty Acids; D007491:Ipronidazole; D008795:Metronidazole; D009593:Nitroimidazoles; D012385:Ronidazole; D000099219:Royal Jelly