RNA polymerase II gene (RPB2) encoding the second largest protein subunit in Phaeosphaeria nodorum and P. avenaria.
A 5586 bp sequence (accession no. DQ278491), which includes the RNA polymerase II gene (RPB2) encoding the second largest protein subunit (RPB2), was obtained from the wheat biotype Phaeosphaeria nodorum (PN-w) by PCR amplification. The 3841 bp full length RPB2 gene contains two exons and a 52 bp intron, and encodes a complete 1262 amino acid protein. Similar to the C-terminals of the beta subunits of prokaryotes and yeast RNA polymerases, the deduced RPB2 protein contained many structural features needed for gene transcription. Based on the phylogenetic analysis with the deduced RPB2 polypeptide sequences, the PN-w was closely related to the maize pathogen Cochliobolus heterostrophus. Size differences were found in the full length RPB2 gene of cereal Phaeosphaeria species, mainly due to differences in intron size. No nucleotide substitutions were found in homothallic P. avenaria f.sp. triticea (Pat1) and barley biotype P. nodorum (PN-b) isolates used in this study. The nucleotide and deduced amino acid sequences of the RPB2 gene in Pat1 were closely related to that in PN-w.
Malkus
Arkadiusz
A
Department of Plant Pathology, Plant Breeding and Acclimatization Institute, Radzikow, Poland.
Chang
Pi-Fang Linda
PF
Zuzga
Sabina M
SM
Chung
Kuang-Ren
KR
Shao
Jonathan
J
Cunfer
Barry M
BM
Arseniuk
Edward
E
Ueng
Peter P
PP
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D005656:Fungal Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D021122:Protein Subunits / Q000235:genetics; D012319:RNA Polymerase II / Q000235:genetics*; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014908:Triticum / Q000382:microbiology
D005656:Fungal Proteins; D021122:Protein Subunits; D012331:RNA, Fungal; D012319:RNA Polymerase II