[Genetic environment of CTX-M-2 in Klebsiella pneumoniae isolates from hospitalized patients in Uruguay].
We studied two CTX-M-2-producing Klebsiella pneumoniae clinical strains, K96005 and K13, isolated from hospitalized patients in Uruguay, during 1996 and 2003, respectively. The genomic surroundings of bla(CTX-M-2) were characterized by PCR-mapping and DNA sequencing. Our results show that blaCTX-M-2 is included in a complex class-1 integron (InK13), associated with an orf513 in both isolates. The genetic array of the integron, aac(6')-lb, bla(OxA,2), orfD (gene cassette region), associated with an orf513-bla(CTX-M-2), seems to be widely disseminated over the Rio de la Plata region.
Vignoli
R
R
Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Alfredo Navarro 3051, Montevideo, Uruguay.
Cordeiro
N
N
Seija
V
V
Schelotto
F
F
Radice
M
M
Ayala
J
J
Power
P
P
Gutkind
G
G
D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D032023:Integrons; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014583:Uruguay; D001618:beta-Lactamases / Q000235:genetics*
D004269:DNA, Bacterial; C071167:beta-lactamase CTX-2; C515384:beta-lactamase CTX-M-2, Klebsiella pneumoniae; D001618:beta-Lactamases