[Screening of microorganism producing polyunsaturated fatty acids with sugarcane molasses].
A strain name LB1 producing polyunsaturated fatty acids with sugarcane molasses was screened form soil samples by the methods of Sudan Black B stain and the determination of PUFAs' iodine value. Based on the grass morphological and physiological characteristics and the sequence similarity of ribosomal DNA-ITS, the strain was identified as Mucor recurvus sp.. The results of L64 (4(3)) orthogonal experiments indicated when Mucor recurvus was cultivated at 28 degrees C for 5 days with 160 r/min, the yields of PUFAs with 10(0) BX sugarcane molasses (pH 6.0) were up to 80.49% content of total fatty acids. The total lipids content was 57.08% of fermentation biomass, which were composed of 15.42% Oleic acid, 14.38% Linolenic, 23.55% gamma-Linolenic, 3.06% alpha-Linolenic, 9.87% Arachidonic acid, 8.14% Eicosapentaenoic acid, 6.07% Docosahexaenoic acid, etc.
Li
Nan
N
College of Life Science and Technology, Guangxi University, Nanning 530004, China. linan97690612@163.com
Deng
Zhi-nian
ZN
Qin
Yong-ling
YL
Ling
Min
M
Liang
Zhi-qun
ZQ
D002849:Chromatography, Gas; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D008965:Molasses; D009090:Mucorales / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D031786:Saccharum / Q000378:metabolism*; D013696:Temperature
D005231:Fatty Acids, Unsaturated