title: Early phase of reperfusion of human kidney allograft does not affect an erythrocyte anti-oxidative system.
abstract: BACKGROUND
  Generation of reactive oxygen specimens is the basic mechanism leading to ischaemia/reperfusion injury of the kidney graft. Oxygen burst is a trigger for sophisticated biochemical changes leading to generation of oxygenated lipids and changes in microcirculation, which recruit recipient's neutrophils and contribute to delayed graft function. It has been shown that the free radicals generation correlates with the activity of anti-oxidative system. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione (GSH) are involved in protection against free radicals.
  
  
  OBJECTIVE
  To examine the activity of erythrocyte anti-oxidative system during reperfusion of the transplanted kidney allograft.
  
  
  METHODS
  The study included 40 renal transplant recipients. Blood was taken from the iliac vein before transplantation and from the graft's renal vein immediately, as well as 2 and 4 min after total reperfusion. The authors assessed the process of reperfusion using ThermaCAM SC500 termovision camera. Spectrophotometric methods were used to measure superoxide dismutase, glutathione peroxidase and catalase activity as well as glutathione concentrations in erythrocytes.
  
  
  RESULTS
  There were no statistically significant differences in the activities of superoxide dismutase, catalase and glutathione peroxidase as well as glutathione concentrations during the first 4 min after total graft reperfusion. Nevertheless, there was a positive correlation between the activity of superoxide dismutase and glutathione peroxidase.
  
  
  CONCLUSIONS
  The results suggest that the erythrocyte anti-oxidative system is stable during the early phase after reperfusion. An association between some anti-oxidative enzymes was noted.
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczeci, Poland. domanle@sci.pam.szczecin.pl
authors.forename: Leszek
authors.initials: L
authors.lastname: Domański
authors.forename: Barbara
authors.initials: B
authors.lastname: Dołgowska
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Safranow
authors.forename: Marek
authors.initials: M
authors.lastname: Myślak
authors.forename: Jacek
authors.initials: J
authors.lastname: Rózański
authors.forename: Maciej
authors.initials: M
authors.lastname: Romanowski
authors.forename: Marek
authors.initials: M
authors.lastname: Ostrowski
authors.forename: Maciej
authors.initials: M
authors.lastname: Domański
authors.forename: Andrzej
authors.initials: A
authors.lastname: Pawlik
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Ciechanowski
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007084:Iliac Vein; D007668:Kidney / Q000378:metabolism / Q000601:surgery; D007676:Kidney Failure, Chronic / Q000378:metabolism / Q000601:surgery*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D015427:Reperfusion Injury / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014184:Transplantation, Homologous
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase