title: Adiponectin inhibits superoxide generation by human neutrophils.
abstract: Adiponectin (Ad), a member of the adipocytokine family, has been reported to possess antiinflammatory properties. We investigated the effects of full-length human Ad (hAd) on phorbol 12-myristate 13-acetate (PMA)-induced O2-* generation by human neutrophils. hAd, even at the lowest tested concentration of 0.001 microg/ml, after 30-min pretreatment of cells, significantly inhibited O2-* generation by neutrophils stimulated with PMA (100 nM). However, no relation between the dose of hAd and extent of inhibition of PMA-induced O2-* generation was observed with increasing the concentration of hAd up to 1 microg/ml. hAd also significantly inhibited neutrophil O2-* generation stimulated by N-formyl-methionyl-leucyl-phenylalanine (100 microM) and diacylglycerol (500 nM), as well as the PMA-induced neutrophil nitroblue tetrazolium reduction and H2O2 formation. Pretreatment of neutrophils with pronase-digested hAd failed to inhibit the PMA-induced O2-* generation. For the first time, this study revealed that Ad inhibited O2-* generation by neutrophils, possibly through regulation of NADPH oxidase.
authors.forename: Ulysses J
authors.initials: UJ
authors.lastname: Magalang
authors.forename: Ramya
authors.initials: R
authors.lastname: Rajappan
authors.forename: Melissa G
authors.initials: MG
authors.lastname: Hunter
authors.forename: Vijay K
authors.initials: VK
authors.lastname: Kutala
authors.forename: Periannan
authors.initials: P
authors.lastname: Kuppusamy
authors.forename: Mark D
authors.initials: MD
authors.lastname: Wewers
authors.forename: Clay B
authors.initials: CB
authors.lastname: Marsh
authors.forename: Narasimham L
authors.initials: NL
authors.lastname: Parinandi
mesh_terms: D052242:Adiponectin / Q000494:pharmacology; D004075:Diglycerides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D013481:Superoxides / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology; D013997:Time Factors
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D004075:Diglycerides; D011994:Recombinant Proteins; D013481:Superoxides; C016658:phorbolol myristate acetate; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate