title: High-performance liquid chromatographic enantioseparations on capillary columns containing crosslinked polysaccharide phenylcarbamate derivatives attached to monolithic silica.
abstract: Monolithic capillary columns containing native silica gel were covalently modified with 3,5-disubstituted phenylcarbamate derivatives of cellulose and amylose and applied for enantioseparations in capillary LC. The method previously used for covalent immobilization of polysaccharide phenylcarbamate derivatives onto the surface of microparticulate silica gel was successfully adapted for in situ modification of monolithic fused-silica capillary columns. The effects of the nature of polysaccharide and the substituents, as well as of multiple covalent immobilization of polysaccharide derivative on chromatographic performance of capillary columns were studied. The capillary columns obtained using this technique are stable in all solvents commonly used in LC and exhibit promising enantiomer resolving ability.
authors.affiliation: Molecular Recognition and Separation Science Laboratory, School of Chemistry, Tbilisi State University, Tbilisi, Georgia, USA.
authors.forename: Bezhan
authors.initials: B
authors.lastname: Chankvetadze
authors.forename: Takateru
authors.initials: T
authors.lastname: Kubota
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Ikai
authors.forename: Chiyo
authors.initials: C
authors.lastname: Yamamoto
authors.forename: Masami
authors.initials: M
authors.lastname: Kamigaito
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tanaka
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Okamoto
mesh_terms: D000688:Amylose / Q000031:analogs & derivatives / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003432:Cross-Linking Reagents; D005782:Gels; D015394:Molecular Structure; D048448:Phenylcarbamates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D003432:Cross-Linking Reagents; D005782:Gels; D048448:Phenylcarbamates; D011134:Polysaccharides; D058428:Silica Gel; D012822:Silicon Dioxide; D002482:Cellulose; D000688:Amylose