The Pax2/5/8 gene egl-38 coordinates organogenesis of the C. elegans egg-laying system.
Organogenesis requires coordinated development between different tissues and cells. The Pax family of transcription factors coordinates multiple developmental events in organs including the kidney, thyroid and the eye. Studying Pax factors in different organisms should identify unifying characteristics of organ development with implications to both development and disease. Here we investigate the function of the Pax2/5/8 transcription factor EGL-38 in coordinating development of the C. elegans egg-laying system. A functional egg-laying system requires cell fate specification events in the epithelial cells of the vulva as well as the mesodermal cells in the uterus of the somatic gonad. Using gene expression studies, genetic mutant analysis and genetic mosaics, we show that egl-38 has functions in both tissues of the organ to promote its development. We incorporate these results together with previous results to propose that EGL-38 plays multiple roles in the development of the egg-laying system, acting to both promote cell fate and to coordinate the development between different cell types. As the Pax2 gene performs similar roles in the development of the mammalian kidney, we show that coordinating organogenesis is a conserved function for Pax2/5/8 transcription factors.
Rajakumar
Vandana
V
Department of Molecular Genetics, Ohio State University, 938 Biological Sciences Building, Columbus, OH 43210, USA.
Chamberlin
Helen M
HM
D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics; D005260:Female; D008969:Molecular Sequence Data; D038081:Organogenesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014599:Uterus / Q000378:metabolism; D014844:Vulva / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; D014157:Transcription Factors; C497470:egl-38 protein, C elegans; D066246:ErbB Receptors; C085873:let-23 protein, C elegans