Antiangiogenic activity of a concentrated effective microorganism fermentation extract.
We have previously demonstrated the possible growth inhibitory activity of both first generation of the effective microorganism fermentation extract (EM-X) as well as the second generation (EM-X2) on cancer cell lines in vitro. The possible anti-angiogenic potential of EM-X has not been reported. Herein we show that using the concentrated EM-X, the growth of human umbilical cord endothelial cells (HUCE) was significantly inhibited in vitro. Enzyme linked immunosorbent assay suggested that the concentrated EM-X is able to reduce the level of vascular endothelial growth factor (VEGF) from Hep3B hepatocellular carcinoma (HCC) cells. The conditioned culture medium obtained from the concentrated EM-X incubated Hep3B HCC cells possessed significant antiproliferative effect on the HUCE cells. Moreover, in vivo chick chorioallantoic membrane assay further demonstrated that the concentrated EM-X is able to greatly inhibit the basic fibroblast growth factor induced angiogenesis from chick embryo experiment. We speculate that the anti-cancer potential of this concentrated EM-X involved growth inhibition on cancer cell and antiangiogenic effect on HUCE cells.
Chui
Chung Hin
CH
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, PR China.
Gambari
Roberto
R
Lau
Fung Yi
FY
Hau
Desmond Kwok Po
DK
Wong
Raymond Siu Ming
RS
Cheng
Gregory Yin Ming
GY
Kok
Stanton Hon Lung
SH
Higa
Teruo
T
Ke
Bin
B
Chan
Albert Sun Chi
AS
Fong
David Wan Fun
DW
Tang
Johnny Cheuk On
JC
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002642:Chick Embryo; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned; D015536:Down-Regulation; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D014470:Umbilical Cord / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000037:antagonists & inhibitors*
D020533:Angiogenesis Inhibitors; D017077:Culture Media, Conditioned; D010936:Plant Extracts; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C508178:microorganism fermentation extract