Establishment of a gene expression system in Ochrobactrum anthropi.
Genetic studies of Ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. We constructed a set of vectors containing several promoters and a His tag fusion in the N terminus to facilitate protein detection and purification. The new vectors should significantly enhance the genetic manipulation and characterization of O. anthropi.
Seleem
Mohamed N
MN
Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic and State University, Blacksburg, VA 24060, USA.
Ali
Mohammed
M
Boyle
Stephen M
SM
Mukhopadhyay
Biswarup
B
Witonsky
Sharon G
SG
Schurig
Gerhardt G
GG
Sriranganathan
Nammalwar
N
D015870:Gene Expression / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005798:Genes, Bacterial / Q000502:physiology; D008969:Molecular Sequence Data; D020621:Ochrobactrum anthropi / Q000235:genetics* / Q000378:metabolism