[The study on angiogenesis activity of danggui, chuanxiong and danshen].
OBJECTIVE
To observe the effect of danggui (Radix angelicae sinensis), chuanxiong (Rhizoma chuanxiong) and danshen (Radix salvae miltionrrhizae) on cardiac microvascular endothelial cells (CMECs) obtained from rat and quantitation of vessels on chick embryo chorioallantoic membrane (CAM) model.


METHODS
Normal rat cardiac microvascular endothelial cells (CMECs) were cultured by collagenase and trypsin and the influences of the herbs on the CMECs were observed by cell count and MTT colorimetry. The activity of blood vessels was determined by quantitation of vessels on chick embryo chorioallantoic membrane (CAM) model.


RESULTS
Compared with the normal group, after treatment with chuanxiong of high dosage, danggui of high and middle dosages, danshen of high and middle and low dosages, they enhanced proliferation significantly (P < 0.05). The two later could be in dependent dose. And the herbs might increase quantitation of vessels on CAM.


CONCLUSIONS
These Chinese herbs may promote angiogenesis by stimulating proliferation of CMEC and incresasing blood vessels.
Meng
Hua
H
Department of Physiology, Xi Jing Hospital, China.
Zhu
Miao-zhang
MZ
Guo
Jun
J
Sun
Ji-yuan
JY
Pei
Jian-ming
JM
Huang
Chen
C
D000482:Allantois / Q000098:blood supply / Q000502:physiology; D029971:Angelica sinensis / Q000737:chemistry; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005434:Flow Cytometry; D008297:Male; D009206:Myocardium / Q000166:cytology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry
D004365:Drugs, Chinese Herbal