Conservative inheritance of newly synthesized DNA in double-strand break-induced gene conversion.
To distinguish among possible mechanisms of repair of a double-strand break (DSB) by gene conversion in budding yeast, Saccharomyces cerevisiae, we employed isotope density transfer to analyze budding yeast mating type (MAT) gene switching in G2/M-arrested cells. Both of the newly synthesized DNA strands created during gene conversion are found at the repaired locus, leaving the donor unchanged. These results support suggestions that mitotic DSBs are primarily repaired by a synthesis-dependent strand-annealing mechanism. We also show that the proportion of crossing-over associated with DSB-induced ectopic recombination is not affected by the presence of nonhomologous sequences at one or both ends of the DSB or the presence of additional sequences that must be copied from the donor.
Ira
Grzegorz
G
Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02453-2728, USA.
Satory
Dominik
D
Haber
James E
JE
D017124:Conserved Sequence / Q000235:genetics*; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage / Q000235:genetics*; D004271:DNA, Fungal / Q000096:biosynthesis* / Q000235:genetics*; D005785:Gene Conversion / Q000235:genetics*; D049770:Genes, Mating Type, Fungal / Q000235:genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
D004271:DNA, Fungal