title: Thrombin induces rapid PAR1-mediated non-classical FGF1 release.
abstract: Thrombin induces cell proliferation and migration during vascular injury. We report that thrombin rapidly stimulated expression and release of the pro-angiogenic polypeptide fibroblast growth factor 1 (FGF1). Thrombin failed to induce FGF1 release from protease-activated receptor 1 (PAR1) null fibroblasts, indicating that this effect was dependent on PAR1. Similarly to thrombin, FGF1 expression and release were induced by TRAP, a specific oligopeptide agonist of PAR1. These results identify a novel aspect of the crosstalk between FGF and thrombin signaling pathways which both play important roles in tissue repair and angiogenesis.
authors.affiliation: Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Duarte
authors.forename: Vihren
authors.initials: V
authors.lastname: Kolev
authors.forename: Raffaella
authors.initials: R
authors.lastname: Soldi
authors.forename: Alexander
authors.initials: A
authors.lastname: Kirov
authors.forename: Irene
authors.initials: I
authors.lastname: Graziani
authors.forename: Silvia Marta
authors.initials: SM
authors.lastname: Oliveira
authors.forename: Doreen
authors.initials: D
authors.lastname: Kacer
authors.forename: Robert
authors.initials: R
authors.lastname: Friesel
authors.forename: Thomas
authors.initials: T
authors.lastname: Maciag
authors.forename: Igor
authors.initials: I
authors.lastname: Prudovsky
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D044463:Receptor, PAR-1 / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013917:Thrombin / Q000008:administration & dosage*
chemical_list: D044463:Receptor, PAR-1; D016220:Fibroblast Growth Factor 1; D013917:Thrombin