title: Sustained Notch signaling in progenitors is required for sequential emergence of distinct cell lineages during organogenesis.
abstract: Mammalian organogenesis results from the concerted actions of signaling pathways in progenitor cells that induce a hierarchy of regulated transcription factors critical for organ and cell type determination. Here we demonstrate that sustained Notch activity is required for the temporal maintenance of specific cohorts of proliferating progenitors, which underlies the ability to specify late-arising cell lineages during pituitary organogenesis. Conditional deletion of Rbp-J, which encodes the major mediator of the Notch pathway, leads to premature differentiation of progenitor cells, a phenotype recapitulated by loss of the basic helix-loop-helix (bHLH) factor Hes1, as well as a conversion of the late (Pit1) lineage into the early (corticotrope) lineage. Notch signaling is required for maintaining expression of the tissue-specific paired-like homeodomain transcription factor, Prop1, which is required for generation of the Pit1 lineage. Attenuation of Notch signaling is necessary for terminal differentiation in post-mitotic Pit1+ cells, and the Notch-repressed Pit1 target gene, Math3, is specifically required for maturation and proliferation of the GH-producing somatotrope. Thus, sustained Notch signaling in progenitor cells is required to prevent conversion of the late-arising cell lineages to early-born cell lineages, permitting specification of diverse cell types, a strategy likely to be widely used in mammalian organogenesis.
authors.affiliation: Howard Hughes Medical Institute, Department and School of Medicine, University of California at San Diego, La Jolla, California 92093, USA.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Jessica
authors.initials: J
authors.lastname: Tollkuhn
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Ohsawa
authors.forename: Emery H
authors.initials: EH
authors.lastname: Bresnick
authors.forename: François
authors.initials: F
authors.lastname: Guillemot
authors.forename: Ryoichiro
authors.initials: R
authors.lastname: Kageyama
authors.forename: Michael G
authors.initials: MG
authors.lastname: Rosenfeld
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D019070:Cell Lineage / Q000235:genetics / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D038081:Organogenesis / Q000235:genetics / Q000502:physiology*; D010902:Pituitary Gland / Q000196:embryology / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D050817:Transcription Factor Pit-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D051880:Receptors, Notch; D050817:Transcription Factor Pit-1; C104516:Neurod4 protein, mouse; C102134:Prophet of Pit-1 protein