Swimming stress in DN 14-3-3 mice triggers maladaptive cardiac remodeling: role of p38 MAPK.
It is generally believed that a mechanical signal initiates a cascade of biological events leading to coordinated cardiac remodeling. 14-3-3 family members are dimeric phosphoserine-binding proteins that regulate signal transduction, apoptotic, and checkpoint control pathways. To evaluate the molecular mechanism underlying swimming stress-induced cardiac remodeling, we examined the role of 14-3-3 protein and MAPK pathway by pharmacological and genetic means using transgenic mice with cardiac-specific expression of dominant-negative (DN) mutants of 14-3-3 (DN 14-3-3/TG) and p38alpha/beta MAPK (DNp38alpha and DNp38beta) mice. p38 MAPK activation was earlier, more marked, and longer in the myocardium of the TG group compared with that of the nontransgenic (NTG) group after swimming stress, whereas JNK activation was detected on day 5 and decreased afterward. In contrast, ERK1/2 was not activated after swimming stress in either group. Cardiomyocyte apoptosis, cardiac hypertrophy, and fibrosis were greatly increased in the TG group compared with those in the NTG group. Moreover, we found a significant correlation between p38 MAPK activation and apoptosis in the TG group. Furthermore, DN 14-3-3 hearts showed enhanced atrial natriuretic peptide expression. In contrast, DNp38alpha and DNp38beta mice exhibited reduced mortality and increased resistance to cardiac remodeling after 28 days of swimming stress compared with TG and NTG mice. Besides, treatment with a p38 MAPK inhibitor, FR-167653, resulted in regression of cardiac hypertrophy and fibrosis and improvement in the survival rate in the TG group. These results indicate for the first time that 14-3-3 protein along with p38 MAPK plays a crucial role in left ventricular remodeling associated with swimming stress.
Watanabe
Ken-ichi
K
Dept of Clinical Pharmacology, Niigata Univ of Pharmacy and Applied Life Sciences, Higashijima, Niigata City, Japan. watanabe@niigata-pharm.ac.jp
Ma
Meilei
M
Hirabayashi
Ken-ichi
K
Gurusamy
Narasimman
N
Veeraveedu
Punniyakoti T
PT
Prakash
Paras
P
Zhang
Shaosong
S
Muslin
Anthony J
AJ
Kodama
Makoto
M
Aizawa
Yoshifusa
Y
D048948:14-3-3 Proteins / Q000235:genetics / Q000502:physiology*; D000818:Animals; D006331:Heart Diseases / Q000503:physiopathology*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009206:Myocardium / Q000201:enzymology*; D013312:Stress, Physiological / Q000503:physiopathology*; D016019:Survival Analysis; D013550:Swimming / Q000502:physiology*; D018487:Ventricular Dysfunction, Left / Q000503:physiopathology*; D016277:Ventricular Function, Left; D020257:Ventricular Remodeling; D048051:p38 Mitogen-Activated Protein Kinases / Q000502:physiology*
D048948:14-3-3 Proteins; D048051:p38 Mitogen-Activated Protein Kinases