Crystallization and preliminary X-ray crystallographic studies of pig heart carbonyl reductase.
Pig heart carbonyl reductase (PHCR), which belongs to the short-chain dehydrogenase/reductase (SDR) family, has been crystallized by the hanging-drop vapour-diffusion method. Two crystal forms (I and II) have been obtained in the presence of NADPH. Form I crystals belong to the tetragonal space group P4(2), with unit-cell parameters a = b = 109.61, c = 94.31 A, and diffract to 1.5 A resolution. Form II crystals belong to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 120.10, c = 147.00 A, and diffract to 2.2 A resolution. Both crystal forms are suitable for X-ray structure analysis at high resolution.
Aoki
Ken-ichi
K
School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.
Tanaka
Nobutada
N
Ishikura
Shuhei
S
Araki
Naoko
N
Imamura
Yorishige
Y
Hara
Akira
A
Nakamura
Kazuo T
KT
D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D009249:NADP / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D034421:Sus scrofa
D011994:Recombinant Proteins; D009249:NADP; D000429:Alcohol Oxidoreductases