[Expression of PPAR-gamma in human hilar bile duct carcinoma cell line QBC939 and the effects of PPAR-Y activated by its ligand on the growth of QBC939].
OBJECTIVE
To explore the expression of Peroxisome Proliferator-activated Receptor-gamma (PPAR-gamma) in QBC939 and the effects of PPAR-gamma activated by its ligand pioglitazone on the growth of human bile duct carcinoma cell line.


METHODS
QBC939 cells were cultured and treated with different concentration of pioglitazone; the expression of PPAR-gamma mRNA was detected by RT-PCR; the effects of PPAR-gamma activated by its ligand on cell proliferation were examined by cell count under light microscope; the influences of activated PPAR-gamma on cell cycle were examined by flow cytometry, and the apoptosis of cancer cells induced by PPAR-gamma ligand pioglitazone was detected by flow cytometry and TUNEL methods.


RESULTS
PPAR-gamma was expressed in human hilar bile duct carcinoma cell line QBC939. And after PPAR-gamma was activated by its ligand pioglitazone, it significantly inhibited cell proliferation, produced G2/M phase arrest and induced apoptosis of QBC939.


CONCLUSIONS
PPAR-gamma, after being activated by its ligand pioglitazone, can inhibit the cell growth of QBC939 remarkably through suppression of cell proliferation, increase in proportion of G2/M phase cells and induction of apoptosis, so PPAR-gamma may be a molecular therapeutical target against the human bile duct carcinoma.
Cheng
Wen
W
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Cheng
Nan-sheng
NS
Xiong
Xian-ze
XZ
Xia
Qing-jie
QJ
Chen
Oing-ying
OY
Yan
Nai-hong
NH
Jing
Jing
J
D017209:Apoptosis / Q000502:physiology; D001650:Bile Duct Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008024:Ligands; D047495:PPAR gamma / Q000096:biosynthesis* / Q000235:genetics; D000077205:Pioglitazone; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D045162:Thiazolidinediones / Q000494:pharmacology*
D008024:Ligands; D047495:PPAR gamma; D012333:RNA, Messenger; D045162:Thiazolidinediones; D000077205:Pioglitazone