Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity.
Lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The activity of the pretreated lipase immobilized covalently on a silica gel was 530 U/g-matrix, which is 16 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.
Lee
Dong Hwan
DH
Department of Chemical and Biological Engineering, Korea University, Anam-dong, Sungbuk-ku, Seoul, Korea.
Kim
Jung Mo
JM
Kang
Seong Woo
SW
Lee
Ji Won
JW
Kim
Seung Wook
SW
D001665:Binding Sites; D001671:Biochemistry / Q000379:methods; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005227:Fatty Acids / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry; D008049:Lipase / Q000737:chemistry*; D008957:Models, Genetic; D012822:Silicon Dioxide / Q000737:chemistry; D013024:Soybean Oil / Q000378:metabolism*; D013237:Stereoisomerism; D013997:Time Factors
D004800:Enzymes, Immobilized; D005227:Fatty Acids; D012822:Silicon Dioxide; D013024:Soybean Oil; D008049:Lipase; D005990:Glycerol