title: Assessment of DNA damage and repair in Mycobacterium terrae after exposure to UV irradiation.
abstract: OBJECTIVE
  Ultraviolet (UV) irradiation for drinking water treatment was examined for inactivation and subsequent dark and photo-repair of Mycobacterium terrae.
  
  
  RESULTS
  UV sources tested were low pressure (monochromatic, 254 nm) and medium pressure (polychromatic UV output) Hg lamps. UV exposure resulted in inactivation, and was followed by dark or photo-repair experiments. Inactivation and repair were quantified utilizing a molecular-based endonuclease sensitive site (ESS) assay and conventional colony forming unit (CFU) viability assay. Mycobacterium terrae was more resistant to UV disinfection compared to many other bacteria, with approximately 2-log reduction at a UV fluence of 10 mJ cm(-2) ; similar to UV inactivation of M. tuberculosis. There was no difference in inactivation between monochromatic or polychromatic UV lamps. Mycobacterium terrae did not undergo detectable dark repair. Photo-repair resulted in recovery from inactivation by approximately 0.5-log in less than 30 min for both UV lamp systems.
  
  
  CONCLUSIONS
  Mycobacterium terrae is able to photo-repair DNA damage within a short timeframe. The number of pyrimidine dimers induced by UV light were similar for Escherichia coli and M. terrae, however, this similarity did not hold true for viability results.
  
  
  CONCLUSIONS
  There is no practical difference between UV sources for disinfection or prevention of DNA repair for M. terrae. The capability of M. terrae to photo-repair UV damage fairly quickly is important for wastewater treatment applications where disinfected effluent is exposed to sunlight. Finally, molecular based assay results should be evaluated with respect to differences in the nucleic acid content of the test micro-organism.
authors.affiliation: Department of Civil and Environmental Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Bohrerova
authors.forename: K G
authors.initials: KG
authors.lastname: Linden
mesh_terms: D015169:Colony Count, Microbial; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects*; D004203:Disinfection / Q000379:methods; D004307:Dose-Response Relationship, Radiation; D009170:Nontuberculous Mycobacteria / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial