Detection of Helicobacter spp. DNA in the oral cavity of dogs.
The mode of acquisition of gastric Helicobacter spp. infection in dogs has not been determined. It is suspected that oral-oral and faecal-oral transmission may be involved. The present study sought to determine if Helicobacter spp. DNA is present in the oral cavity of healthy and vomiting dogs. Thirty-eight pet dogs (27 vomiting and 11 clinically healthy) were studied. The presence of Helicobacter spp. was determined by single and nested PCR evaluation of DNA extracted from saliva, dental plaque and gastric biopsy samples. Helicobacter spp. DNA was detected by nested PCR in 36 (94.7%) gastric biopsies, 17 (44.7%) dental plaque and 19 (50%) saliva samples out of the 38 dogs examined. Overall 27 (71.1%) dogs screened by nested PCR were found to harbour Helicobacter spp. DNA in the oral cavity (dental plaque and/or saliva). There was no significant difference in the prevalence of Helicobacter spp. DNA in the oral cavity of vomiting and healthy dogs, and the time from vomiting to oral sampling did not have significant impact. This study confirms the high prevalence of gastric Helicobacter spp. infection in dogs, and reveals that Helicobacter spp. DNA is detectable in the oral cavity of over 70% of dogs. These findings support the possibility of oral-oral transmission between dogs and that the canine oral cavity may act as source of non-pylori Helicobacter spp. infection for humans.
Recordati
Camilla
C
Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Sezione di Anatomia Patologica Veterinaria e Patologia Aviare, Facoltà di Medicina Veterinaria, Università degli Studi di Milano, Milano, Italy. camilla.recordati@unimi.it
Gualdi
Valentina
V
Tosi
Sabrina
S
Facchini
Roberto Vailati
RV
Pengo
Graziano
G
Luini
Mario
M
Simpson
Kenneth W
KW
Scanziani
Eugenio
E
D000818:Animals; D016022:Case-Control Studies; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology; D004197:Disease Reservoirs / Q000662:veterinary; D004283:Dog Diseases / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D004285:Dogs; D005260:Female; D016998:Helicobacter / Q000235:genetics / Q000302:isolation & purification*; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D006801:Humans; D008297:Male; D009055:Mouth / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D015995:Prevalence; D012463:Saliva / Q000382:microbiology; D014839:Vomiting / Q000382:microbiology / Q000662:veterinary; D015047:Zoonoses
D004269:DNA, Bacterial