Rose (Rosa hybrida L.).
Although rose transformation is successful, it remains difficult to transform myriad rose species as well as different rose genotypes. In this protocol, a detailed description of rose transformation is presented. This protocol relied on Agrobacterium-mediated transfer of embryogenic callus cultures. There are many critical steps that must be followed to achieve successful transformation; however, it is important to keep in mind that these apply to a selected number of genotypes, and as a different genotype is subjected to transformation, modifications of this protocol must be made to achieve successful transformation.
Korban
Schuyler S
SS
Department of Natural Resources and Environmental Sciences, 310 ERML, University of Illinois, Urbana, IL, USA.
Gasic
Ksenija
K
Li
Xiangqian
X
D000064:Acclimatization; D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D018929:Cell Culture Techniques; D003470:Culture Media; D005838:Genotype; D012038:Regeneration; D027826:Rosa / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
D003470:Culture Media