Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L.
The defatted seed meal of common vetch (Vicia sativa L.) served as a source of (R)-oxynitrilase which catalyzed the enantioselective addition of HCN to aromatic, heteroaromatic, fluoro-substituted aromatic aldehydes to produce the corresponding enantiomeric pure cyanohydrins in yields of 52-100% and 88-99% ee at 12 degrees C under micro-aqueous medium (diisopropyl ether) without addition of buffer solution.
Han
Shi-Qing
SQ
College of Pharmacy and Life Science, Nanjing University of Technology, Nanjing, China. hanshiqing@njut.edu.cn
Ouyang
Ping-Kai
PK
Wei
Ping
P
Hu
Yi
Y
D000446:Aldehyde-Lyases / Q000138:chemical synthesis / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods; D001711:Biotransformation; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009570:Nitriles / Q000138:chemical synthesis / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D010944:Plants / Q000378:metabolism; D013237:Stereoisomerism; D013696:Temperature; D033022:Vicia sativa / Q000201:enzymology*
D009570:Nitriles; D010936:Plant Extracts; C430593:cyanohydrin; D000446:Aldehyde-Lyases; C021541:mandelonitrile lyase