title: [Relationship between MC4R gene polymorphyism and body weight trait in beagle dogs].
abstract: The relationship between MC4R gene polymorphism and body weight in beagle dogs was analyzed. Using gene-specific primers based on canine MC4R exonic sequences a gene fragment was PCR amplified, cloned and sequenced to identify potential polymorphisms. The relationship between a MC4R gene polymorphism detected by PCR-RFLP and canine body weight was analyzed. Three variants were found in beagle dog MC4R DNA sequence, of which two were deletions and one was a transversion which created a PshA I site that could be detected by PCR-RFLP. A statistically significant relationship between this polymorphism and body weight was found. MC4R gene could be a candidate modifier gene for canine body weight.
authors.affiliation: Department of Immunology and Pathogenic Biology, Jinzhou Medical College, Jinzhou 121001, China. jyzyb@yahoo.com.cn
authors.forename: Yi-Bo
authors.initials: YB
authors.lastname: Zhang
authors.forename: Cai-Feng
authors.initials: CF
authors.lastname: Ba
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Su
authors.forename: Rui-Xia
authors.initials: RX
authors.lastname: Zeng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics*; D004285:Dogs / Q000235:genetics* / Q000502:physiology*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D044105:Receptor, Melanocortin, Type 4 / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D044105:Receptor, Melanocortin, Type 4