Different expression patterns of TRP genes in murine B and T lymphocytes.
A prolonged increase in the intracellular calcium concentration ([Ca2+]i) is essential for lymphocyte activation that includes cell proliferation and differentiation. This increase in [Ca2+]i results from Ca2+ release from the intracellular store and the subsequent Ca2+ influx from the extracellular environment via calcium channels located on the plasma membrane. Although transient receptor potential (TRP) channels have been reported to play important roles in the [Ca2+]i increase in lymphocytes, the function of these channels in lymphocyte activation remains unknown. Here, we report the comprehensive expression profile of TRP channel gene families including TRPC, TRPV, and TRPM in the murine immune system. RT-PCR analysis revealed different expression patterns of the TRP channel genes in B and T lymphocytes isolated from the spleen. Therefore, our results provide an appropriate reference of TRP gene expression in murine lymphocytes.
Inada
Hitoshi
H
Section of Cell Signaling, Department of Bio-environmental Science, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Science, Higashiyama 5-1, Myodaiji, Okazaki, Aichi 444-8787, Japan.
Iida
Tohko
T
Tominaga
Makoto
M
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000378:metabolism*; D050051:Transient Receptor Potential Channels / Q000378:metabolism*
D050051:Transient Receptor Potential Channels