title: Immobilization of biomaterials to nano-assembled films (self-assembled monolayers, Langmuir-Blodgett films, and layer-by-layer assemblies) and their related functions.
abstract: For utilization of highly sophisticated functions of biomaterials in nano-scale functional systems, immobilization of biomaterials on artificial devices such as electrodes via thin film technology is one of the most powerful strategies. In this review, we focus on three major organic ultrathin films, self-assembled monolayers (SAM), Langmuir-Blodgett (LB) films, and layer-by-layer (LBL) assemblies, and from the viewpoints of biomaterial immobilization, typical examples and recent progresses in these film technologies are described. The SAM method allows facile contact between biomaterials and man-made devices, and well used for bio-related sensors. In addition, recent micro-fabrication techniques such as micro-contact printing and dip-pen nanolithography were successfully applied to preparation of biomaterial patterning. A monolayer at the air-water interface, which is a unit structure of LB films, provides a unique environment for recognition of aqueous biomaterials. Recognition and immobilization of various biomaterials including nucleotides, nucleic acid bases, amino acids, sugars, and peptides were widely investigated. The LB film can be also used for immobilization of enzymes in an ultrathin film on an electrode, resulting in sensor application. The LBL assembling method is available for wide range of biomaterials and provides great freedom in designs of layered structures. These advantages are reflected in preparation of thin-film bio-reactors where multiple kinds of enzymes sequentially operate. LBL assemblies were also utilized for sensors and drug delivery systems. This kind of assembling structures can be prepared on micro-size particle and very useful for preparation of hollow capsules with biological functions.
authors.affiliation: Supermolecules Group, National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Ariga
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Michinobu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D040761:Biomimetic Materials; D015374:Biosensing Techniques; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008567:Membranes, Artificial; D008956:Models, Chemical; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011108:Polymers; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; D008055:Lipids; D008567:Membranes, Artificial; D011108:Polymers