title: Antifungal effect of amentoflavone derived from Selaginella tamariscina.
abstract: Amentoflavone is a plant bif avonoid that was isolated from an ethyl acetate extract of the whole plant of Selaginella tamariscina (Beauv.) spring. 1D and 2D NMR spectroscopy including DEPT, HMQC, and HMBC were used to determine its structure. Amentoflavone exhibited potent antifungal activity against several pathogenic fungal strains but had a very low hemolytic effect on human erythrocytes. In particular, amentoflavone induced the accumulation of intracellular trehalose on C. albicans as a stress response to the drug, and disrupted the dimorphic transition that forms pseudo-hyphae during pathogenesis. In conclusion, amentoflavone has great potential to be a lead compound for the development of antifungal agents.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Hyun Jun
authors.initials: HJ
authors.lastname: Jung
authors.forename: Woo Sang
authors.initials: WS
authors.lastname: Sung
authors.forename: Soo-Hwan
authors.initials: SH
authors.lastname: Yeo
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: In-Seon
authors.initials: IS
authors.lastname: Lee
authors.forename: Eun-Rhan
authors.initials: ER
authors.lastname: Woo
authors.forename: Dong Gun
authors.initials: DG
authors.lastname: Lee
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D044946:Biflavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D015169:Colony Count, Microbial; D004912:Erythrocytes / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D032503:Selaginellaceae / Q000737:chemistry*; D014199:Trehalose / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D044946:Biflavonoids; D000666:Amphotericin B; C011164:amentoflavone; D014199:Trehalose