title: [Quantification for biofilm formation of pseudomonas aeruginosa isolates and their genotypic analysis].
abstract: OBJECTIVE
  To probe the correlation between the ability of biofilm formation of Pseudomonas aeruginosa isolates and their genotypes.
  
  
  METHODS
  Forty-eight Pseudomonas aeruginosa isolates were tested for their biofilm formation with a modified microtiter test and were analyzed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The percent similarity between their genetic fingerprints and cluster analysis was performed and worked out using computer software.
  
  
  RESULTS
  Forty-eight Pseudomonas aeruginosa isolates demonstrated different abilities of biofilm formation in vitro. And the 48 isolates with different abilities of biofilm formation showed different genotypes in fingerprints by ERIC-PCR. Among the 48 isolates of Pseudomonas aeruginosa tested, five clusters (A, B, C, D, E), were identified at the 17% genetic similarity level. The isolates with strong ability of biofilm formation were in D cluster at the 42% genetic similarity level.
  
  
  CONCLUSIONS
  Most of the 48 PA isolates tested formed strong biofilm, and their genotypes were of correlation.
authors.affiliation: Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Cheng
authors.forename: Wei-qing
authors.initials: WQ
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Yin
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Wei
authors.initials: W
authors.lastname: Zeng
authors.forename: Xin-hui
authors.initials: XH
authors.lastname: Li
authors.forename: Jian-yu
authors.initials: JY
authors.lastname: Su
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Kou
authors.forename: Wen-xiang
authors.initials: WX
authors.lastname: Jia
mesh_terms: D018441:Biofilms; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000502:physiology*; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D004269:DNA, Bacterial