title: Crystallization and preliminary X-ray analysis of Atg3.
abstract: Atg3 is an E2-like enzyme that catalyzes the conjugation reaction between Atg8 and phosphatidylethanolamine (PE). The Atg8-PE conjugate is essential for autophagy, the bulk degradation process of cytoplasmic components by the vacuolar/lysosomal system. Crystals of Saccharomyces cerevisiae Atg3 have been obtained by the sitting-drop vapour-diffusion method using ammonium sulfate and lithium sulfate as precipitants. A native data set was collected from a single crystal to 2.5 A resolution. The crystals belong to space group P4(1) or P4(3), with unit-cell parameters a = 59.33, c = 115.22 A, and are expected to contain one protein molecule per asymmetric unit.
authors.affiliation: Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, N-12, W-6, Kita-ku, Sapporo 060-0812, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Yamada
authors.forename: Nobuo N
authors.initials: NN
authors.lastname: Suzuki
authors.forename: Yuko
authors.initials: Y
authors.lastname: Fujioka
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ichimura
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ohsumi
authors.forename: Fuyuhiko
authors.initials: F
authors.lastname: Inagaki
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D000071183:Autophagy-Related Proteins; D003460:Crystallization; D018360:Crystallography, X-Ray; D018020:Lithium Compounds / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D013431:Sulfates / Q000737:chemistry; D044763:Ubiquitin-Conjugating Enzymes
chemical_list: D000071183:Autophagy-Related Proteins; D018020:Lithium Compounds; D029701:Saccharomyces cerevisiae Proteins; D013431:Sulfates; C054097:lithium sulfate; D044763:Ubiquitin-Conjugating Enzymes; C104235:ATG3 protein, S cerevisiae; D000645:Ammonium Sulfate