Cork Oak Trees (Quercus suber L.).
A transformation system for selected mature Quercus suber L. trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries the nptII and uidA genes. Evidence of stable transgene integration is obtained by polymerase chain reaction for nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos are germinated and successfully transferred to soil.
Alvarez
Rubén
R
Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, C/. Catedrático Rodrigo Uría Sn, Oviedo, Spain.
Toribio
Mariano
M
Cortizo
Millán
M
Ordás Fernández
Ricardo-Javier
RJ
D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D015139:Blotting, Southern; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D003470:Culture Media; D018525:Germination; D005966:Glucuronidase / Q000032:analysis; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000196:embryology / Q000254:growth & development; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D029963:Quercus / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D012987:Soil; D014170:Transformation, Genetic; D019076:Transgenes
D003470:Culture Media; D012987:Soil; D005966:Glucuronidase