[Excretion of (-)-clausenamide in rats].
OBJECTIVE
To study the excretion of (-)-clausenamide in rats.


METHODS
The urine, feces and bile were collected at predetermined time points after (-)-clausenamide was orally administrated to 6 rats (30 mg x kg(-1)). The concentrations of (-)-clausenamide and its metabolite 6-OH-(-)-clausnamide were determined by HPLC-MS/MS method using glipzide as the internal reference, and the accumulative excretion amount of (-)-clausenamide and 6-OH-(-)-clausenamide was calculated in the urine, feces and bile, separately.


RESULTS
(-)-Clausenamide was recovered mostly (44%) from feces in 112 hours, 7.1% was found from urine in 120 hours and 0.013% was detected from bile in 24 hours. The accumulative excretions of 6-OH-(-)-clausenamide were 0.92% , 0.46% and 0.0003% of the administered dose from feces, urine and bile, respectively.


CONCLUSIONS
The major amount of (-)-clausenamide was recovered from feces after (-)-clausenamide was orally administrated to rats (30 mg kg(-1)).
Qian
Wen
W
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.
Wang
Lei-na
LN
Song
Min
M
Zheng
Xiu-wen
XW
Hang
Tai-jun
TJ
Zhang
Zheng-xing
ZX
D000284:Administration, Oral; D000818:Animals; D001646:Bile / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D054700:Clausena / Q000737:chemistry*; D005243:Feces / Q000737:chemistry*; D005260:Female; D007769:Lactams / Q000737:chemistry / Q000493:pharmacokinetics* / Q000652:urine; D017705:Lignans / Q000737:chemistry / Q000493:pharmacokinetics* / Q000652:urine; D008297:Male; D013058:Mass Spectrometry; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism
D007769:Lactams; D017705:Lignans; D018696:Neuroprotective Agents; C053048:clausenamide