Cyanobacterial ecotypes in the microbial mat community of Mushroom Spring (Yellowstone National Park, Wyoming) as species-like units linking microbial community composition, structure and function.
We have investigated microbial mats of alkaline siliceous hot springs in Yellowstone National Park as natural model communities to learn how microbial populations group into species-like fundamental units. Here, we bring together empirical patterns of the distribution of molecular variation in predominant mat cyanobacterial populations, theory-based modelling of how to demarcate phylogenetic clusters that correspond to ecological species and the dynamic patterns of the physical and chemical microenvironments these populations inhabit and towards which they have evolved adaptations. We show that putative ecotypes predicted by the theory-based model correspond well with distribution patterns, suggesting populations with distinct ecologies, as expected of ecological species. Further, we show that increased molecular resolution enhances our ability to detect ecotypes in this way, though yet higher molecular resolution is probably needed to detect all ecotypes in this microbial community.
Ward
David M
DM
Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT 59717, USA. umbdw@montana.edu
Bateson
Mary M
MM
Ferris
Michael J
MJ
Kühl
Michael
M
Wieland
Andrea
A
Koeppel
Alex
A
Cohan
Frederick M
FM
D000220:Adaptation, Biological / Q000235:genetics*; D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics* / Q000502:physiology*; D017753:Ecosystem; D014644:Genetic Variation; D008954:Models, Biological; D008969:Molecular Sequence Data; D010100:Oxygen / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013057:Spectrum Analysis; D013696:Temperature; D014959:Wyoming
D012336:RNA, Ribosomal, 16S; D010100:Oxygen