Comparison of two capillary electrophoresis online stacking modes by analysis of polycyclic aromatic hydrocarbons in airborne particulates.
Naphthalene, fluorene, pyrene, anthracene, phenanthrene, and chrysene were successfully separated by CD-modified MEKC (CD-MEKC) using 20 mM borate (pH 9.0) containing 90 mM SDS and 75 mM beta-CD. Two online stacking methods, i.e., sweeping and field-enhanced sample injection (FESI), were explored to enhance the detection sensitivity. The influences of some crucial parameters in sweeping and FESI procedures were investigated. For FESI method, a plug of water and low-conductivity sample matrix was used to increase the stacking efficiency. Compared with the sweeping method, FESI can increase the sensitivity in the range of 10-20-fold. The proposed method was used for the analysis of polycyclic aromatic hydrocarbons in airborne particulates.
Song
Guan-Qun
GQ
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, P. R. China.
Peng
Zhen-Lei
ZL
Lin
Jin-Ming
JM
D000393:Air Pollutants / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods* / Q000706:statistics & numerical data; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D009862:Online Systems; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D012680:Sensitivity and Specificity; D047392:beta-Cyclodextrins
D000393:Air Pollutants; D011084:Polycyclic Aromatic Hydrocarbons; D047392:beta-Cyclodextrins; C031215:betadex