Signature-tagged transposon mutagenesis identifies novel Mycobacterium tuberculosis genes involved in the parasitism of human macrophages.
Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.
Rosas-Magallanes
Vania
V
Unit of Mycobacterial Genetics, Institut Pasteur, Paris, France.
Stadthagen-Gomez
Gustavo
G
Rauzier
Jean
J
Barreiro
Luis B
LB
Tailleux
Ludovic
L
Boudou
Frédéric
F
Griffin
Ruth
R
Nigou
Jérome
J
Jackson
Mary
M
Gicquel
Brigitte
B
Neyrolles
Olivier
O
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics*; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D008264:Macrophages / Q000469:parasitology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016296:Mutagenesis; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000472:pathogenicity*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements