title: Cassava (Manihot esculenta Crantz).
abstract: During the last three years the generation of stably transformed cassava plants having value-added traits has become a reality. Currently, two Agrobacterium-mediated transformation systems are routinely used to engineer cassava. These systems use either somatic embryos or friable embryogenic calli. This paper presents detailed protocols for the transformation of cassava using primary somatic embryos. The effects of explant types, tissue culture conditions, and bacterial and plasmid related factors on transformation efficiency are discussed.
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA.
authors.forename: Weston
authors.initials: W
authors.lastname: Msikita
authors.forename: Uzoma
authors.initials: U
authors.lastname: Ihemere
authors.forename: Dimuth
authors.initials: D
authors.lastname: Siritunga
authors.forename: Richard T
authors.initials: RT
authors.lastname: Sayre
mesh_terms: D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D003470:Culture Media; D005818:Genetic Engineering; D018525:Germination; D002365:Manihot / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D040901:Proteomics; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
chemical_list: D003470:Culture Media; D010940:Plant Proteins