Isolation and characterization of a novel uronic acid-containing acidic glycosphingolipid from the ascidian Halocynthia roretzi.
A novel uronic acid-containing glycosphingolipid (UGL-1) was isolated from the ascidian Halocynthia roretzi. UGL-1 was prepared from chloroform-methanol extracts and purified by the use of successive column chromatography on DEAE-Sephadex, Florisil, and Iatrobeads. Chemical structural analysis was performed using methylation analysis, gas chromatography, gas chromatography-mass spectrometry, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry, and 1H-NMR spectra. The chemical structure of UGL-1 was determined to be a glucuronic acid-containing glycosphingolipid, Galbeta1-4(Fucalpha1-3)GlcAbeta1-1Cer. The ceramide component was composed of C16:0 and C18:0 acids and C16-, C17-, and C18-phytosphingosines as major components.
Ito
Masahiro
M
Department of Bioscience and Bioinformatics, College of Information Science and Engineering, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu, Shiga 525-8577, Japan. maito@is.ritsumei.ac.jp
Matsumuro
Yuki
Y
Yamada
So
S
Kitamura
Tomonori
T
Itonori
Saki
S
Sugita
Mutsumi
M
D000818:Animals; D002241:Carbohydrates / Q000032:analysis / Q000302:isolation & purification; D002255:Carbonic Acid / Q000032:analysis; D002518:Ceramides / Q000737:chemistry; D006028:Glycosphingolipids / Q000737:chemistry / Q000302:isolation & purification*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014561:Urochordata / Q000737:chemistry*; D014574:Uronic Acids / Q000737:chemistry
D002241:Carbohydrates; D002518:Ceramides; D006028:Glycosphingolipids; D014574:Uronic Acids; D002255:Carbonic Acid