[Harmine induces apoptosis in human SGC-7901 cells].
OBJECTIVE
[corrected] To investigate the effect of apoptosis induced in human SGC-7901 cells by Harmine.


METHODS
The effect of Harmine on human SGC-7901 cell survival and apoptosis was determined by MTT assay, light microscopy and flow cytometry. Cell genomic DNA was detected by agarose electrophoresis.


RESULTS
The survival of human SGC-7901 cells decreased; Apoptotic cells were observed by fluorescent microscope; FCM analysis showed that the peak of apoptosis increased. Typical DNA Ladder were detected in DNA agarose gel electrophoresis.


CONCLUSIONS
HM can induce apoptosis in human SGC-7901 cells.
Song
Zhen-ya
ZY
Department of Gastroenterology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.
Liu
Ji-ren
JR
Lu
Xin-liang
XL
Wang
Liang-jing
LJ
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival; D005434:Flow Cytometry; D006247:Harmine / Q000494:pharmacology*; D006801:Humans; D032443:Peganum / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D013274:Stomach Neoplasms / Q000473:pathology
D000972:Antineoplastic Agents, Phytogenic; D006247:Harmine