Identification of the FtsK sequence-recognition domain.
FtsK is a prokaryotic multidomain DNA translocase that coordinates chromosome segregation and cell division. FtsK is membrane anchored at the division septum and, guided by highly skewed DNA sequences, translocates the chromosome to bring the terminus of replication to the septum. Here, we use in vitro single-molecule and ensemble methods to unveil a mechanism of action in which the translocation and sequence-recognition activities are performed by different domains in FtsK.
Ptacin
Jerod L
JL
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA.
Nöllmann
Marcelo
M
Bustamante
Carlos
C
Cozzarelli
Nicholas R
NR
D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary
D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C096452:FtsK protein, E coli; D008565:Membrane Proteins