[Primary study on acid tolerance mechanism of a wild aciduric Rhizobium strain isolated from Pueraria lobata].
An aciduric Rhizobium strain, named as PR389, was isolated from the nodule of wild Pueraria lobata which grew in yellow soil (pH 4.6) on the Jin-yun Mountain in Bei-bei, Chong-qing city. The isolated strain, which could grow under pH 4.6 distinct from the optimal pH 6.5-7.5 for rhizobium, showed some typical features of aciduric rhizobium. This was also confirmed by the proton flux assay. Compared to the acid-sensitive Rhizobium strain PR21, the cell membrane of PR389 could hold back excessive H entering cell. This feature can protect PR389 from harm of acid. In the test of acid tolerance, the aciduric ability of strain PR389 under low acidic (pH 3.8) was restrained by antibiotic chloramphenicol. It was speculated that special proteins in the cells of PR389 could be induced and synthesized in acidic environment.
Gu
Jian-ping
JP
College of Resource and Environment, Southwest University, Chongqing 400716, China. gwping80@163.com
Zhang
Lei
L
Wei
Shi-qing
SQ
Zhang
Qin
Q
Fang
De-hua
DH
D000143:Acids / Q000494:pharmacology*; D004361:Drug Tolerance; D006863:Hydrogen-Ion Concentration; D029908:Pueraria / Q000382:microbiology*; D012231:Rhizobium / Q000187:drug effects* / Q000302:isolation & purification*; D053204:Root Nodules, Plant / Q000382:microbiology*
D000143:Acids