title: Assembly of multilayer films incorporating a viral protein cage architecture.
abstract: Protein cage architectures such as viral capsids, heat shock proteins, ferritins, and DNA-binding proteins are nanoscale modular subunits that can be used to expand the structural and functional range of composite materials. Here, layer-by-layer (LbL) assembly was used to incorporate cowpea chlorotic mottle virus (CCMV) into multilayer films. Three types of multilayer films were prepared. In the first type, ionic interactions were employed to assemble CCMV into triple layers. In the second type, complementary biological interactions (streptavidin/biotin) were used for this purpose. In a third variation of LbL assembly, complementary biological interactions were employed to produce nanotextured films that exhibit in-plane order over a micron scale without the need to adsorb onto a prepatterned template.
authors.affiliation: Department of Microbiology, Montana State University, Bozeman, Montana 59717, USA.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Suci
authors.forename: Michael T
authors.initials: MT
authors.lastname: Klem
authors.forename: Fernando T
authors.initials: FT
authors.lastname: Arce
authors.forename: Trevor
authors.initials: T
authors.lastname: Douglas
authors.forename: Mark
authors.initials: M
authors.lastname: Young
mesh_terms: D001710:Biotin / Q000737:chemistry; D017795:Bromovirus; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D017550:Spectroscopy, Fourier Transform Infrared; D014764:Viral Proteins / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D014764:Viral Proteins; D001710:Biotin