title: Sensitive and rapid electrochemical bioassay of glycosidase activity.
abstract: Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.
authors.affiliation: The Biodesign Institute and Fulton School of Engineering, Arizona State University, Tempe, AZ 85287-5801, USA.
authors.forename: Tanin
authors.initials: T
authors.lastname: Tangkuaram
authors.forename: Jared Q
authors.initials: JQ
authors.lastname: Gerlach
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.forename: Abdel-Nasser
authors.initials: AN
authors.lastname: Kawde
authors.forename: Zong
authors.initials: Z
authors.lastname: Dai
authors.forename: Veer P
authors.initials: VP
authors.lastname: Bhavanandan
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: La Belle
authors.forename: Waret
authors.initials: W
authors.lastname: Veerasai
authors.forename: Lokesh
authors.initials: L
authors.lastname: Joshi
authors.forename: Joseph
authors.initials: J
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008362:Mannosides / Q000378:metabolism; D009596:Nitrophenols / Q000032:analysis / Q000378:metabolism
chemical_list: D008362:Mannosides; D009596:Nitrophenols; C036119:4-nitrophenyl-beta-D-mannopyranoside; D006026:Glycoside Hydrolases