title: Toward intelligent nanosize bioreactors: a pH-switchable, channel-equipped, functional polymer nanocontainer.
abstract: To develop an intelligent sensor-effector functionality on the nanoscale, a pH-switchable, controlled nanoreactor based on amphiphilic copolymer membranes was built. The nanovesicles were equipped with bacterial transmembrane ompF pore proteins and the pH-sensitive enzyme acid phosphatase, resulting in a switchable substrate processing at pH 4-6.5. Ideal pH and substrate concentrations for the reaction were determined experimentally. In future, the reactor might be used for self-regulating targeted diagnostic and therapeutic applications in medicine.
authors.affiliation: Medical Intensive Care Unit, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland.
authors.forename: Pavel
authors.initials: P
authors.lastname: Broz
authors.forename: Sergey
authors.initials: S
authors.lastname: Driamov
authors.forename: Joerg
authors.initials: J
authors.lastname: Ziegler
authors.forename: Nadav
authors.initials: N
authors.lastname: Ben-Haim
authors.forename: Stephan
authors.initials: S
authors.lastname: Marsch
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Meier
authors.forename: Patrick
authors.initials: P
authors.lastname: Hunziker
mesh_terms: D019149:Bioreactors; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006863:Hydrogen-Ion Concentration; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D011108:Polymers / Q000737:chemistry*; D018272:Porins / Q000737:chemistry*
chemical_list: C109237:OmpF protein; D011108:Polymers; D018272:Porins