title: Development of an HPLC-PAD-MS assay for the identification and quantification of major phenolic edelweiss (Leontopodium alpium Cass.) constituents.
abstract: The analytical assessment of edelweiss (Leontopodium alpinum) herb extracts, used in traditional alpine medicine, has resulted in the development of a HPLC-PAD-MS method that allows baseline separation of almost all constituents. Peak assignment of 14 analytes was achieved by comparison of retention times, UV and mass spectra with those of reference compounds either commercially available (luteolin, apigenin and chlorogenic acid) or isolated from edelweiss plants by column chromatography. Ten of the isolated analytes were identified as the known natural products: quercetin-3-O-beta-D-glucoside, luteolin-7-O-beta-D-glucoside, luteolin-3'-O-beta-D-glucoside, luteolin-4'-O-beta-D-glucoside, apigenin-7-O-beta-D-glucoside, 6-hydroxy-luteolin-7-O-beta-D-glucoside, luteolin-7,4'-di-O-beta-D-glucoside, chrysoeriol-7-O-beta-D-glucoside, leontopodic acid and 3,5-dicaffeolyquinic acid. One analyte, 3,4,5-tri-(E)-caffeoly-D-glucaric acid proved to be a new natural product and was named leontopodic acid B. Structure elucidation was carried out by means of MS and NMR spectroscopy in all cases. The aerial plant parts of L. alpinum (capitula, inflorescence leaves, stems, stem leaves and leaves of the basal rosette) showed variable amounts of the above-mentioned constituents, although qualitative differences were not observable.
authors.affiliation: Institut für Pharmazie/Pharmakognosie, Leopold-Franzens-Universität Innsbruck, Innrain, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwaiger
authors.forename: Christoph
authors.initials: C
authors.lastname: Seger
authors.forename: Bettina
authors.initials: B
authors.lastname: Wiesbauer
authors.forename: Peter
authors.initials: P
authors.lastname: Schneider
authors.forename: Ernst P
authors.initials: EP
authors.lastname: Ellmerer
authors.forename: Sonja
authors.initials: S
authors.lastname: Sturm
authors.forename: Hermann
authors.initials: H
authors.lastname: Stuppner
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry
chemical_list: D010636:Phenols