5-fluoro-orotic acid induces chromosome alterations in genetically manipulated strains of Candida albicans.
We previously reported the occurrence of chromosome alterations in a Candida albicans prototrophic strain 3153A treated with 5-fluoro-orotic acid (5-FOA). In this study we investigated the mutagenic properties of 5-FOA with two derivatives of C. albicans strain CAF4-2 (ura3/ura3), each containing an ectopic copy of URA3 gene (ura3/ ura3 URA3) on a different chromosome. As expected, after the ura3/ura3 URA3 constructs were applied to 5-FOA containing solid medium, the "pop-outs" that lost URA3 appeared. However most of the "pop-outs" acquired various chromosome alterations. Thus constructs exposed to 5-FOA should be examined for chromosome alterations or the use of 5-FOA should be avoided.
Wellington
Melanie
M
Department of Pediatrics, University of Rochester Medical Center, Rochester, NY 14642, USA.
Kabir
M Anaul
MA
Rustchenko
Elena
E
D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics; D043171:Chromosomal Instability; D002869:Chromosome Aberrations; D015825:Chromosomes, Fungal / Q000187:drug effects* / Q000235:genetics; D003470:Culture Media; D016521:Electrophoresis, Gel, Pulsed-Field; D005656:Fungal Proteins / Q000235:genetics; D009154:Mutation; D009963:Orotic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D014498:Uracil
D003470:Culture Media; D005656:Fungal Proteins; D014498:Uracil; D009963:Orotic Acid; C001242:5-fluoroorotic acid