title: Chiral purity test of bevantolol by capillaryelectrophoresis and high performance liquid chromatography.
abstract: Two methods for the chiral purity determination of bevantolol were developed, namely capillary electrophoresis (CE) using carboxymethyl-beta-cyclodextrin (CM-beta-CD) as a chiral selector and high-performance liquid chromatography (HPLC) using a chiral stationary phase. In the HPLC method, the separation of bevantolol enantiomers was performed on a Chiralpak AD-H column by isocratic elution with n-hexane-ethanol-diethylamine (10:90:0.1, v/v/v) as mobile phase. In the CE method, bevantolol enantiomers were separated on an uncoated fused silica capillary with 50 mM amonium phosphate dibasic adjusted to a pH 6.5 with phosphoric acid containing 15 mM CM-beta-CD as running buffer. Validation data such as linearity, recovery, detection limit, and precision of the two methods are presented. The detection limits of S-(-)-bevantolol were 0.1% and 0.05% for CE and HPLC method, respectively and R-(+)-bevantolol were 0.15% and 0.05% for CE and HPLC method, respectively. There was generally good agreement between the HPLC and CE results.
authors.affiliation: College of Pharmacy, Kangwon National University, Chunchon 200-701, Korea.
authors.forename: Pham Hai
authors.initials: PH
authors.lastname: Long
authors.forename: Tran Quoc
authors.initials: TQ
authors.lastname: Trung
authors.forename: Joung Won
authors.initials: JW
authors.lastname: Oh
authors.forename: Kyeong Ho
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000737:chemistry*; D000465:Algorithms; D002851:Chromatography, High Pressure Liquid; D004340:Drug Contamination; D019075:Electrophoresis, Capillary; D011412:Propanolamines / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D013237:Stereoisomerism
chemical_list: D000319:Adrenergic beta-Antagonists; D011412:Propanolamines; C021148:bevantolol