Tall Fescue (Festuca arundinacea Schreb.).
Tall fescue (Festuca arundinacea Schreb.) is the predominant cool-season perennial grass in the United States. It is widely used for both forage and turf purposes. This chapter describes a protocol that allows for the generation of large number of transgenic tall fescue plants by Agrobacterium-mediated transformation. Embryogenic calli induced from caryopsis are used as explants for inoculation with A. tumefaciens. The Agrobacterium strain used is EHA105. Hygromycin phosphotransferase gene (hph) is used as the selectable marker and hygromycin is used as the selection agent. Calli resistant to hygromycin are obtained after 4-6 wk of selection. Soil-grown tall fescue plants can be regenerated 4-5 mo after Agrobacterium-mediated transformation.
Ge
Yaxin
Y
Forage Improvement Division, The Samuel Roberts Noble Foundation, Ardmore, OK, USA.
Wang
Zeng-Yu
ZY
D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D018920:Coculture Techniques; D003470:Culture Media; D031751:Festuca / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D005819:Genetic Markers; D030821:Plants, Genetically Modified / Q000033:anatomy & histology / Q000254:growth & development; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D012987:Soil; D013242:Sterilization; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
D003470:Culture Media; D005819:Genetic Markers; D012987:Soil