Estimation of hydroxyl radical generation by salicylate hydroxylation method in multiple organs of mice exposed to whole-body X-ray irradiation.
Appropriate experimental conditions for the estimation of hydroxyl radical generation by salicylate hydroxylation were determined for multiple organs of X-irradiated mice in vivo. The in vitro experiments showed that there were significant correlations between the salicylic acid (SA) concentration, the amount of 2,3-dihydroxy benzoic acid (2,3-DHBA) and the X-ray exposure dose, and we obtained two linear-regression equations to calculate the amounts of hydroxyl radicals generated by the X-irradiation. The optimum dosage of SA and the appropriate sampling time for in vivo experiments was determined, and significant increases in the ratio of 2,3-DHBA to SA were detected in several organs of mice after X-irradiation. The hydroxyl radical equivalents of the 2,3-DHBA increases were also calculated. Our results clearly demonstrated the usefulness of the salicylate hydroxylation method in estimating hydroxyl radical generation in multiple organs in vivo.
Ueno
Shunji
S
School of Veterinary Medicine and Animal Sciences, Kitasato University, Laboratory of Veterinary Public Health, Higashi 23-35-1, Towada, Japan. ueno@vmas.kitasato-u.ac.jp
Kashimoto
Takashige
T
Susa
Nobuyuki
N
Wada
Kohshin
K
Ito
Nobuhiko
N
Takeda-Homma
Shino
S
Nishimura
Yoshikazu
Y
Sugiyama
Masayasu
M
D000818:Animals; D002396:Catechols / Q000032:analysis / Q000378:metabolism / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D062385:Hydroxybenzoates; D017665:Hydroxyl Radical / Q000032:analysis / Q000378:metabolism* / Q000528:radiation effects; D006900:Hydroxylation; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011833:Radiation Injuries, Experimental; D012459:Salicylates / Q000032:analysis / Q000378:metabolism* / Q000528:radiation effects; D014018:Tissue Distribution; D014916:Whole-Body Irradiation / Q000009:adverse effects; D014965:X-Rays
D002396:Catechols; D062385:Hydroxybenzoates; D012459:Salicylates; D017665:Hydroxyl Radical; C009135:2,3-dihydroxybenzoic acid