Coexpression of an unusual form of the EWS-WT1 fusion transcript and interleukin 2/15 receptor betamRNA in a desmoplastic small round cell tumour.
BACKGROUND
The beta chain of the interleukin 2/15 receptor (IL-2/15Rbeta) is induced by the expression of the EWS-WT1. A case of desmoplastic small round cell tumour (DSRCT) expressing only an unusual EWS-WT1 treated by us is reported here.


OBJECTIVE
To characterise an unusual form of EWS-WT1.


METHODS
Frozen tissue sections of the axillary tumour were examined using a laser-assisted microdissection technique and reverse transcriptase polymerase chain reaction.


RESULTS
The novel fusion of exon 8 of EWS and the defective exon 10 of WT1 (-KTS) was detected. Although it was an unusual form, the coexpression of the present EWS-WT1, IL-2/15Rbeta and Janus kinase (JAK1) mRNA was detected in the tumour cells. IL-2 and signal transducers and activators of transcription (STAT5) mRNA were detected in both tumour and stromal cells.


CONCLUSIONS
The induction of the IL-2/15 receptor signalling pathway may contribute to tumorigenesis in DSRCT through a paracrine or an autocrine system, even though the EWS-WT1 was an unusual form.
Nakanishi
Y
Y
Department of Pathology, Nihon University School of Medicine, Tokyo, Japan.
Oinuma
T
T
Sano
M
M
Fuchinoue
F
F
Komatsu
K
K
Seki
T
T
Obana
Y
Y
Tabata
M
M
Kikuchi
K
K
Shimamura
M
M
Ohmori
K
K
Nemoto
N
N
D000328:Adult; D001483:Base Sequence; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D017809:Fatal Outcome; D006801:Humans; D053646:Interleukin-2 Receptor beta Subunit / Q000096:biosynthesis* / Q000235:genetics; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000378:metabolism; D015514:Oncogene Proteins, Fusion / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
C106032:EWS1-WT1 fusion protein, human; D053646:Interleukin-2 Receptor beta Subunit; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D012333:RNA, Messenger; D012334:RNA, Neoplasm