A comparison of the effectiveness of four biostains in enhancing visualization of the vitreous.
To compare the effectiveness of four biostains in vitreous staining as a means to facilitate clean anterior vitrectomy.



Four biostains (fluorescein, indocyanine green, trypan blue, and triamcinolone acetonide) were used to stain the transparent vitreous of human cadaver and monkey cadaver eyes. Results were compared and recorded by digital photography and videography.



Of the four biostains, triamcinolone acetonide best highlighted the vitreous. Although fluorescein, indocyanine green, and trypan blue also produced acceptable vitreous staining, the surrounding ocular structures were slightly stained as well.



All four biostains enhanced visualization of the vitreous. However, triamcinolone acetonide best highlighted the vitreous.
Guo
Suqin
S
Institute of Ophthalmology and Visual Science, New Jersey Medical School, Newark, New Jersey, USA.
Tutela
Arthur C
AC
Wagner
Rudolph
R
Caputo
Anthony R
AR
D000818:Animals; D004396:Coloring Agents; D019793:Fluorescein; D006801:Humans; D007208:Indocyanine Green; D008251:Macaca; D013194:Staining and Labeling; D014222:Triamcinolone Acetonide; D014343:Trypan Blue; D014822:Vitreous Body / Q000033:anatomy & histology*
D004396:Coloring Agents; D014222:Triamcinolone Acetonide; D014343:Trypan Blue; D007208:Indocyanine Green; D019793:Fluorescein