Feasibility of freeze-dried sera for serological and molecular biological detection of hepatitis B and C viruses.
We compared hepatitis B virus (HBV) surface antigen, anti-hepatitis C virus (HCV) antibody, and HCV RNA quantification in frozen and freeze-dried serum samples to assess the usefulness of freeze-dried sera for detection of HBV and HCV. The results indicated that freeze-dried sera as well as frozen sera can be useful for serological and molecular biological analyses of HBV and HCV.
Ohishi
Waka
W
Department of Clinical Studies, Radiation Effects Research Foundation, 5-2 Hijiyama Park, Minami-ku, Hiroshima, 732-0815, Japan. nwaka@rerf.or.jp
Fujiwara
Saeko
S
Suzuki
Gen
G
Kishi
Takeshi
T
Sora
Misae
M
Matsuura
Shinsuke
S
Hakoda
Masayuki
M
Tatsukawa
Yoshimi
Y
Yamada
Michiko
M
Chayama
Kazuaki
K
D005612:Freeze Drying; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006509:Hepatitis B / Q000175:diagnosis / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis / Q000821:virology; D018937:Hepatitis C Antibodies / Q000032:analysis; D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044967:Serum / Q000821:virology*; D013048:Specimen Handling / Q000379:methods*
D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies; D012367:RNA, Viral