A simple method for extracting C-phycocyanin from Spirulina platensis using Klebsiella pneumoniae.
C-phycocyanin (C-PC) was extracted from fresh Spirulina platensis by deploying a species of non-pathogenic nitrogen-fixing bacteria, namely, Klebsiella pneumoniae. The algal slurry was neither washed nor centrifuged; the bacterial culture was poured into the slurry, the vessel sealed, and crude C-PC extracted after about 24 h. The extraction was clean and efficient, and the purity and concentration of C-PC proved to be of adequate quality.
Zhu
Y
Y
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Photosynthesis Research Center, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Chen
X B
XB
Wang
K B
KB
Li
Y X
YX
Bai
K Z
KZ
Kuang
T Y
TY
Ji
H B
HB
D001426:Bacterial Proteins / Q000737:chemistry*; D001433:Bacteriolysis; D003470:Culture Media; D005615:Freezing; D005898:Glass; D007218:Industrial Microbiology / Q000379:methods; D007711:Klebsiella pneumoniae / Q000254:growth & development* / Q000502:physiology; D008863:Microspheres; D009113:Muramidase / Q000378:metabolism; D009586:Nitrogen Fixation; D010798:Phycocyanin / Q000302:isolation & purification*; D013010:Sonication; D053545:Spirulina / Q000737:chemistry* / Q000254:growth & development / Q000502:physiology*
D001426:Bacterial Proteins; D003470:Culture Media; D010798:Phycocyanin; D009113:Muramidase