[Expression of mucosal addressin cell adhesion molecule-1 during small bowel graft rejection in rat].
OBJECTIVE
To investigate the expression of mucosal addressin cell adhesion molecule-1(MAdCAM-1) during small bowel graft rejection and the effects of MAdCAM-1 on the development of acute rejection.


METHODS
Rat heterotopic small bowel transplantation (SBT) was performed in F344/N rats with syngeneic and allogeneic (BN-F344/N) grafts. Bowel and gut-associated lymphoid tissue(GALT) samples were collected from small bowel transplants on postoperative day(POD) 1, 3, 5 and 7. Histopathology assessment of the grafts was conducted to identify the rejection. MAdCAM-1 was detected by immunohistochemistry and Western blot.


RESULTS
During acute rejection, MAdCAM-1 was highly-expressed on gut lamina propia and GALTs, particularly on vascular endothelial cells in the gut lamina propia. There were no change of MAdCAM-1 expression in syngeneic grafts from POD1 to POD7. In allogeneic grafts, MAdCAM-1 expression in mesenteric lymph nodes was down-regulated, while up-regulated on the vascular endothelial cells in the lamina propria during acute rejection.


CONCLUSIONS
Alteration of MAdCAM-1 expression may be associated with the development of SBT graft rejection.
Liu
Ji
J
Department of Gastrointestinal Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710033, China.
Wang
Wei-zhong
WZ
Guan
Wen-xian
WX
Chen
Dong-li
DL
Li
Ji-peng
JP
D000818:Animals; D006084:Graft Rejection / Q000276:immunology / Q000378:metabolism*; D006085:Graft Survival; D007136:Immunoglobulins / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology; D007421:Intestine, Small / Q000637:transplantation*; D008198:Lymph Nodes / Q000378:metabolism*; D008221:Lymphoid Tissue; D008297:Male; D009088:Mucoproteins / Q000378:metabolism*; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344
D007136:Immunoglobulins; C504268:Madcam1 protein, rat; D009088:Mucoproteins