title: Crystallization and preliminary X-ray crystallographic studies of pig heart carbonyl reductase.
abstract: Pig heart carbonyl reductase (PHCR), which belongs to the short-chain dehydrogenase/reductase (SDR) family, has been crystallized by the hanging-drop vapour-diffusion method. Two crystal forms (I and II) have been obtained in the presence of NADPH. Form I crystals belong to the tetragonal space group P4(2), with unit-cell parameters a = b = 109.61, c = 94.31 A, and diffract to 1.5 A resolution. Form II crystals belong to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 120.10, c = 147.00 A, and diffract to 2.2 A resolution. Both crystal forms are suitable for X-ray structure analysis at high resolution.
authors.affiliation: School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Aoki
authors.forename: Nobutada
authors.initials: N
authors.lastname: Tanaka
authors.forename: Shuhei
authors.initials: S
authors.lastname: Ishikura
authors.forename: Naoko
authors.initials: N
authors.lastname: Araki
authors.forename: Yorishige
authors.initials: Y
authors.lastname: Imamura
authors.forename: Akira
authors.initials: A
authors.lastname: Hara
authors.forename: Kazuo T
authors.initials: KT
authors.lastname: Nakamura
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D009249:NADP / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D034421:Sus scrofa
chemical_list: D011994:Recombinant Proteins; D009249:NADP; D000429:Alcohol Oxidoreductases