title: Role of 1-hydroxybenzotriazole in oxidation by laccase from Trametes versicolor. Kinetic analysis of the laccase-1-hydroxybenzotriazole couple.
abstract: In the current studies, we used Lineweaver-Burke analysis to examine the role of 1-hydroxybenzotriazole (HBT) in the oxidation of various compounds by laccase from Trametes versicolor. At low concentrations, HBT was a competitive inhibitor of the oxidation, but at high concentrations, it was a noncompetitive inhibitor. Analysis of the oxidation of ferrocytochrome c by the laccase-HBT couple showed that increasing the concentration of ferrocytochrome c did not affect the V(max) but reduced the apparent K(m). In addition, in the manganese peroxidase-Mn(II) reaction, which is a typical oxidation system by mediator, the apparent K(m) and V(max) increased as the concentration of the substrate 2,6-dimethoxyphenol was increased. These results indicate that HBT is involved in the binding of laccase and substrates that laccase cannot oxidize alone.
authors.affiliation: Department of Forest Resources Science, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan. afhhirai@agr.shizuoka.ac.jp
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hirai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shibata
authors.forename: Shingo
authors.initials: S
authors.lastname: Kawai
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Nishida
mesh_terms: D001487:Basidiomycota / Q000201:enzymology* / Q000378:metabolism; D001667:Binding, Competitive; D045304:Cytochromes c / Q000737:chemistry / Q000378:metabolism; D006139:Guaiacol / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D042845:Laccase / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D014230:Triazoles / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014230:Triazoles; D006139:Guaiacol; D045304:Cytochromes c; C011852:1-hydroxybenzotriazole; D042845:Laccase