Identification and characterization of a major cell wall-associated iron-regulated envelope protein (Irep-28) in Mycobacterium tuberculosis.
Iron limitation and the expression of mycobactin and carboxymycobactin by Mycobacterium tuberculosis are known. Here, we report how iron regulated the coordinate expression of these two siderophores and a 28-kDa cell wall-associated iron-regulated protein (Irep-28). Irep-28 is identified as the DNA-binding HU homologue HupB protein (hupB [Rv2986c]). Antibodies to this protein were detected in sera from tuberculosis patients. The location of the protein in the cell wall makes it a potential drug target.
Yeruva
Veena C
VC
School of Life Sciences, University of Hyderabad, Hyderabad-500 046, India.
Duggirala
Sridevi
S
Lakshmi
V
V
Kolarich
Daniel
D
Altmann
Friedrich
F
Sritharan
Manjula
M
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000502:physiology*; D033862:Iron-Binding Proteins; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D010080:Oxazoles / Q000378:metabolism; D033902:Periplasmic Binding Proteins
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D033862:Iron-Binding Proteins; D010080:Oxazoles; D033902:Periplasmic Binding Proteins; C018608:mycobactins; D007501:Iron