Release of LL-37 by activated human Vgamma9Vdelta2 T cells: a microbicidal weapon against Brucella suis.
Human Vgamma9Vdelta2 T cells play a crucial role in early immune response to intracellular pathogens. Moreover, in brucellosis, these cells are drastically increased in the peripheral blood of patients during the acute phase of infection. In vitro, Vgamma9Vdelta2 T cells are capable of inhibiting Brucella growth and development through a combination of mechanisms: 1) cytotoxicity, 2) macrophage activation and bactericidal activity through cytokine and chemokine secretion, and 3) antibacterial effects. We previously described that antibacterial factors were found in supernatants from activated Vgamma9Vdelta2 T cells. In this study, we show that Vgamma9Vdelta2 T cells express the human cathelicidin hCAP18 and its mature form, known as LL-37, is released upon activation of Vgamma9Vdelta2 T cells. We also show that LL-37 has an antibacterial effect on Brucella suis. Overall, our results demonstrate that LL-37 is a soluble factor responsible for a part of the bactericidal activity of Vgamma9Vdelta2 T cells.
Dudal
Sherri
S
Institut National de la Santé et de la Recherche Médicale, Unité 431, Université Montpellier 2, Montpellier, France.
Turriere
Chrystell
C
Bessoles
Stephanie
S
Fontes
Pascaline
P
Sanchez
Françoise
F
Liautard
Janny
J
Liautard
Jean-Pierre
JP
Lafont
Virginie
V
D023181:Antimicrobial Cationic Peptides / Q000276:immunology*; D001770:Blood Bactericidal Activity; D041021:Brucella suis / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D007113:Immunity, Innate; D008213:Lymphocyte Activation; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D054804:Cathelicidins
D023181:Antimicrobial Cationic Peptides; D016692:Receptors, Antigen, T-Cell, gamma-delta; D054804:Cathelicidins