[Determination of ginsenoside Rd and its metabolites in rat urine by LC-MS].
OBJECTIVE
To study the metabolic pathways of ginsenoside Rd in rats.


METHODS
Urine samples were collected before and after 24 h of single oral administration of 150 mg and intravenous administration of 60 mg of ginsenoside Rd to six rats, separately. The samples were purified by SPE column and then were analyzed by liquid chromatography-ESI-mass spectrometry for putative metabolites.


RESULTS
Parent drug and its seven metabolites were identified in rat urine based on comparing total ion chromatograms of the blank with the metalolic urine as well as mass spectra. Its main metabolic pathways and possible structures are elucidated.


CONCLUSIONS
Oxidation, combination and deglucosylation were found to be the major metabolic pathway of ginsenoside Rd in rats.
Yang
Liu
L
Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou University of Traditional Chinese Medicine pathways, Guangzhou 510120, China.
Xu
Shun-jun
SJ
Zeng
Xing
X
Liu
Yi-ming
YM
Deng
Shi-gui
SG
Wu
Zhi-feng
ZF
Ou
Run-mei
RM
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000008:administration & dosage / Q000378:metabolism* / Q000652:urine*; D007275:Injections, Intravenous; D008297:Male; D010084:Oxidation-Reduction; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D036145:Ginsenosides; C097367:ginsenoside Rg3; C442759:ginsenoside Rb1; C055305:ginsenoside Rh2; C049863:ginsenoside Rd