PET of human prostate cancer xenografts in mice with increased uptake of 64CuCl2.
Our objective was to determine whether human prostate cancer xenografts in mice can be localized by PET using 64CuCl2 as a probe (64Cu PET).


METHODS
Athymic mice bearing human prostate cancer xenografts were subjected to 64Cu PET, followed by quantitative analysis of the tracer concentrations and immunohistochemistry study of human copper transporter 1 expression in the tumor tissues.


RESULTS
Human prostate cancer xenografts expressing high levels of human copper transporter 1 were well visualized on the PET images obtained 24 h after injection but not on the images obtained 1 h after injection. PET quantitative analysis demonstrated a high concentration of 64CuCl2 in the tumors in comparison to that in the left shoulder regions (percentage injected dose per gram of tissue: 3.6 +/- 1.3 and 0.6 +/- 0.3, respectively; P = 0.004), at 24 h after injection.


CONCLUSIONS
The data from this study suggested that locally recurrent prostate cancer might be localized with 64Cu PET using 64CuCl2 as a probe.
Peng
Fangyu
F
Department of Pediatrics, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA. fpeng@pet.wayne.edu
Lu
Xin
X
Janisse
James
J
Muzik
Otto
O
Shields
Anthony F
AF
D000818:Animals; D027682:Cation Transport Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D003301:Copper Radioisotopes / Q000493:pharmacokinetics; D000079923:Copper Transporter 1; D006801:Humans; D008099:Liver / Q000000981:diagnostic imaging / Q000378:metabolism; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D049268:Positron-Emission Tomography; D011467:Prostate / Q000000981:diagnostic imaging / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
D027682:Cation Transport Proteins; D003301:Copper Radioisotopes; D000079923:Copper Transporter 1; D019275:Radiopharmaceuticals; C490079:SLC31A1 protein, human