SECRET AGENT, an Arabidopsis thaliana O-GlcNAc transferase, modifies the Plum pox virus capsid protein.
The capsid protein of Plum pox virus (PPV-CP) is modified with O-linked GlcNAc (O-GlcNAc). While Arabidopsis has two O-GlcNAc transferases, SECRET AGENT (SEC) and SPINDLY (SPY), previous work suggests that SEC modifies PPV-CP and that the modification plays a role in the infection process. Here, we show that when co-expressed in Escherichia coli SEC modifies PPV-CP. Deletion mapping and site-directed mutagenesis identified three threonine and a serine located near the N-terminus of PPV-CP that are modified by SEC. Two of these threonines have recently been shown to be modified in virus from plants suggesting that SEC has the same specificity in plants and E. coli.
Scott
Cheryl L
CL
Department of Plant Biology, University of Minnesota, 1445 Gortner Avenue, 250 Biological Sciences Center, St. Paul, MN 55108, USA.
Hartweck
Lynn M
LM
de Jesús Pérez
José
J
Chen
Dinghu
D
García
Juan Antonio
JA
Olszewski
Neil E
NE
D019943:Amino Acid Substitution; D017360:Arabidopsis / Q000201:enzymology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics; D006031:Glycosylation; D016297:Mutagenesis, Site-Directed; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D017801:Plum Pox Virus / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D012694:Serine / Q000737:chemistry; D013912:Threonine / Q000737:chemistry
D029681:Arabidopsis Proteins; D036022:Capsid Proteins; D004279:DNA, Viral; D011994:Recombinant Proteins; D013912:Threonine; D012694:Serine; D017351:N-Acetylglucosaminyltransferases; C510668:SECRET AGENT protein, Arabidopsis