Interactive effect of genetic polymorphism of glutathione S-transferase M1 and smoking on squamous cell lung cancer risk in Korea.
To evaluate the role of the genetic polymorphisms of CYP2E1, GSTM1 and GSTT1, and their interaction with smoking in lung cancer development in Korean males, a hospital-based case-control study was conducted. Histologically confirmed male lung cancer patients (n=171) and male patients with no present or previous history of systemic illness who visited the urology department (n=196) were recruited from Seoul National University Hospital, Korea (1998-1999). CYP2E1 genotypes were determined by PCR-RFLP using RsaI digestion and GSTM1 and T1 genotypes were determined by multiplex PCR. Risks were estimated as odds ratios (ORs) and 95% confidence intervals (CIs) using a logistic regression model adjusting for age and pack-year. Smoking was a significant risk factor for lung cancer (P<0.001). Although genetic polymorphisms of CYP2E1, GSTM1 and T1 were not associated with the overall risk of lung cancer, the GSTM1 null genotype significantly increased the risk of squamous cell lung cancer (OR=1.9, 95% CI=1.04-3.60). An interactive effect between the GSTM1 null genotype and smoking was observed (P=0.04). These results suggest that the GSTM1 null genotype is associated with squamous cell lung cancer and modifies the effect of smoking on squamous cell lung cancer development in Korean males.
Lee
Kyoung-Mu
KM
Department of Preventive Medicine and Institute of Environmental Medicine, Seoul National University College of Medicine, Cancer Research Institute, Seoul 110-799, Korea.
Kang
Daehee
D
Lee
Seung-Joon
SJ
Park
Sue Kyung
SK
Lee
Kwan-Hee
KH
Choi
Ji-Yeob
JY
Kim
Soo-Ung
SU
Choi
Hwang
H
Choi
Seung-Ho
SH
Kim
Young-Whan
YW
Hong
Yun-Chul
YC
Cho
Soo-Hun
SH
D000328:Adult; D000368:Aged; D003043:Cocarcinogenesis; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007723:Korea; D008175:Lung Neoplasms / Q000201:enzymology / Q000209:etiology* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D018307:Neoplasms, Squamous Cell / Q000201:enzymology / Q000209:etiology* / Q000235:genetics; D011110:Polymorphism, Genetic; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects*
D019392:Cytochrome P-450 CYP2E1; D015252:Deoxyribonucleases, Type II Site-Specific; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1; C040158:GTAC-specific type II deoxyribonucleases