title: Postentry events are responsible for restriction of productive varicella-zoster virus infection in Chinese hamster ovary cells.
abstract: Productive infection of varicella-zoster virus (VZV) in vitro is restricted almost exclusively to cells derived from humans and other primates. We demonstrate that the restriction of productive VZV infection in CHO-K1 cells occurs downstream of virus entry. Entry of VZV into CHO-K1 cells was characterized by utilizing an ICP4/beta-galactosidase reporter gene that has been used previously to study herpes simplex virus type 1 entry. Entry of VZV into CHO-K1 cells involved cell surface interactions with heparan sulfate glycosaminoglycans and a cation-independent mannose-6-phosphate receptor. Lysosomotropic agents inhibited the entry of VZV into CHO-K1 cells, consistent with a low-pH-dependent endocytic mechanism of entry. Infection of CHO-K1 cells by VZV resulted in the production of both immediate early and late gene products, indicating that a block to progeny virus production occurs after the initiation of virus gene expression.
authors.affiliation: Department of Pediatrics, Infectious Diseases Section, Biomedical Research Building 851, C227, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262, USA. renee.finnen@uchsc.edu
authors.forename: Renée L
authors.initials: RL
authors.lastname: Finnen
authors.forename: Kara R
authors.initials: KR
authors.lastname: Mizokami
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Banfield
authors.forename: Guang-Yun
authors.initials: GY
authors.lastname: Cai
authors.forename: Scott A
authors.initials: SA
authors.lastname: Simpson
authors.forename: Lewis I
authors.initials: LI
authors.lastname: Pizer
authors.forename: Myron J
authors.initials: MJ
authors.lastname: Levin
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D004705:Endocytosis; D015870:Gene Expression; D005814:Genes, Viral; D006497:Heparan Sulfate / Q000378:metabolism; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D041681:NIH 3T3 Cells; D017527:Receptor, IGF Type 2 / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism; D013045:Species Specificity; D014779:Virus Replication
chemical_list: D006497:Heparan Sulfate; D017527:Receptor, IGF Type 2; D011991:Receptors, Virus