UbcH10 expression may be a useful tool in the prognosis of ovarian carcinomas.
The UbcH10 gene codes for a protein that belongs to the ubiquitin-conjugating enzyme family. Previous studies of our group suggest UbcH10 expression as a valid indicator of the proliferative and aggressive status of thyroid carcinomas. Therefore, to better understand the process of ovarian carcinogenesis, and to look for possible tools to be used as prognostic markers in these neoplasias, we decided to extend the analysis of the UbcH10 expression to the ovarian neoplastic disease. We found that the UbcH10 gene was upregulated in some ovarian carcinoma cell lines analysed. Then, immunohistochemical studies demonstrate that UbcH10 expression significantly correlates with the tumor grade and the undifferentiated histotype of the ovarian carcinomas. Furthermore, a significant relationship between UbcH10 expression and overall survival was observed. Finally, the block of UbcH10 protein synthesis by RNA interference inhibited the growth of ovarian carcinoma cell lines, suggesting a role of UbcH10 overexpression in ovarian carcinogenesis. Therefore, all these data taken together suggest the possibility to use UbcH10 detection as a marker for the diagnosis and prognosis of these neoplastic diseases and open the perspective of a therapy of some ovarian carcinomas based on the suppression of the UbcH10 synthesis and/or function.
Berlingieri
M T
MT
Dipartimento di Biologia e Patologia Cellulare e Molecolare c/o Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, Facoltà di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli 'Federico II', Naples, Italy.
Pallante
P
P
Guida
M
M
Nappi
C
C
Masciullo
V
V
Scambia
G
G
Ferraro
A
A
Leone
V
V
Sboner
A
A
Barbareschi
M
M
Ferro
A
A
Troncone
G
G
Fusco
A
A
D002277:Carcinoma / Q000175:diagnosis* / Q000401:mortality* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000401:mortality* / Q000473:pathology; D011379:Prognosis; D034622:RNA Interference; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D044763:Ubiquitin-Conjugating Enzymes / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics*; D015854:Up-Regulation
D012333:RNA, Messenger; D034741:RNA, Small Interfering; C105156:UBE2C protein, human; D044763:Ubiquitin-Conjugating Enzymes