One-electron reduction of S-nitrosothiols in aqueous medium.
One-electron reduction of S-nitrosothiols (RSNO) has been studied using radiolytically produced reducing entity, the hydrated electron (e(aq)(-)), in aqueous medium. Both kinetics of the reaction and the mechanistic aspects of the decomposition of S-nitroso derivatives of glutathione, L-cysteine, N-acetyl-L-cysteine, N-acetyl-D,L-penicillamine, N-acetylcysteamine, L-cysteine methyl ester, and D,L-penicillamine have been investigated at neutral and acidic pH. The second-order rate constants of the reaction of e(aq)(-) with RSNOs were determined using a pulse radiolysis technique and were found to be diffusion controlled (10(10) dm(3) mol(-1) s(-1)) at neutral pH. The product analysis using HPLC, fluorimetry, and MS revealed the formation of thiol and nitric oxide as the major end products. It is therefore proposed that one-electron reduction of RSNO leads to the liberation of NO. There is no intermediacy of a thiyl radical as in the case of oxidation reactions of RSNOs. The radical anion of RSNO (RSN(*)O(-)) is proposed as a possible intermediate. The overall reaction could be written as RSNO + e(aq)(-) --H+--> RSH + (*)NO.
Manoj
V M
VM
School of Chemical Sciences, Mahatma Gandhi University, Kottayam 686560, Kerala, India.
Mohan
H
H
Aravind
U K
UK
Aravindakumar
C T
CT
D004583:Electrons; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D026403:S-Nitrosothiols / Q000737:chemistry / Q000378:metabolism*; D014867:Water
D005609:Free Radicals; D026403:S-Nitrosothiols; D014867:Water; D009569:Nitric Oxide