title: Prevalence of diatom frustules in non-vegetarian foodstuffs and its implications in interpreting identification of diatom frustules in drowning cases.
abstract: Detection of diatom frustules in bone marrow (diatom test) is used for diagnosing ante-mortem drowning where the usual signs of drowning are not present in dead bodies recovered from water. However, controversies over the reliability of diatom test results are continuing. There have been indications on the possibilities of diatoms entering into systemic circulation from atmospheric air, food and drink. While diatoms have been demonstrated in the gut content of edible marine forms such as shrimps and clams, the present study, for the first time, provides empirical evidence on the prevalence as well as abundance of diatom frustules in the samples of cooked non-vegetarian foodstuffs that impend human consumption in Kelantan, Malaysia. It is found that 50 g each of cleaned and cooked prawns and of clams impending human consumption contain about 8360 and 29,054 diatom frustules, respectively. A person accustomed to prawn and clam food would be ingesting an estimated 2 million diatoms in a single year. Considering the suggestion that detection of five diatom frustules in 10 g of bone marrow would suffice for concluding drowning as mode of death, and the fact that there is yet no proof that diatom frustules do not enter into the human systemic circulation through the digestive tract, the estimated number of diatom frustules routinely ingested acquires significance since entry of a few of such ingested frustules into the systemic circulation can lead to false positive test results. The findings of this research raise two important issues: first, population based routine food related diatom ingestion requires to be estimated, and, second, studies have to be initiated to categorically prove or disprove the possibility of entry of diatom frustules into the systemic circulation via the digestive tract.
authors.affiliation: Forensic Division, Department of Chemistry, Malaysia, Jalan Sultan, 46661 Petaling Jaya, Selangor Darul Ehsan, Malaysia.
authors.forename: Law Yen
authors.initials: LY
authors.lastname: Yen
authors.forename: P T
authors.initials: PT
authors.lastname: Jayaprakash
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000382:microbiology; D002423:Cause of Death; D033364:Decapoda / Q000382:microbiology; D017377:Diatoms / Q000302:isolation & purification* / Q000648:ultrastructure; D004332:Drowning / Q000175:diagnosis*; D020387:Food Chain; D049429:Forensic Pathology / Q000379:methods; D005766:Gastrointestinal Contents / Q000382:microbiology; D006801:Humans; D008296:Malaysia; D012758:Shellfish / Q000382:microbiology*