Site-specific transition of cytosine to uracil via reversible DNA photoligation.
We report that deamination coupled with 5-carboxyvinyldeoxyuridine-mediated photobranching causes the heat-induced transition of cytosine to uracil with high efficiency without any side reaction.
Fujimoto
Kenzo
K
School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa 923-1292, Japan. kenzo@jaist.ac.jp
Matsuda
Shigeo
S
Yoshimura
Yoshinaga
Y
Matsumura
Takashi
T
Hayashi
Masayuki
M
Saito
Isao
I
D001483:Base Sequence; D003596:Cytosine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010777:Photochemistry; D014498:Uracil / Q000737:chemistry*
D014498:Uracil; D003596:Cytosine; D004247:DNA