[The protective effect of newly synthesized compounds against the action of UV-C irradiation on human lymphocytes in vitro].
In this work we present our data about the protective effect of the newly synthesized compound 1-(4-fluorphenyltioureido)-4-methyl-piperazyne (FTMP) against high doses UV-C irradiation using human lymphocytes in vitro as a model system. The endpoint used was chromosome aberrations. The genotoxic effect of different UV-C doses in the range from 10 J/m2 to 200 J/m2 was evaluated. Studying the protective effect of FTMP, it was obtained that a low, adaptive concentration (10(-6) mol/l) applied before harmful doses of UV-C irradiation (100 J/M2 and 150 J/M2) induces the yield of chromosome aberrations lower than theoretical, estimated as a sum of single effects of both agents. A tendency for reducing the damage effect of UV-C irradiation was established. The effect is the most clear when a 4-hour interval between the treatments was used. The mitotic index was not affected. These results pointed out the ability of FTMP to decrease the damaging effect of UV-C irradiation in this type of cells and possess the potential to induce the adaptive response against the damaging effect of UV-C irradiation.
Gateva
S P
SP
Ionova
P A
PA
Iovchev
G
G
Stergios
M L
ML
Kulekova
S S
SS
D000222:Adaptation, Physiological / Q000187:drug effects; D002869:Chromosome Aberrations; D004249:DNA Damage / Q000187:drug effects*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects; D008940:Mitotic Index; D010879:Piperazines / Q000737:chemistry / Q000494:pharmacology; D013473:Sunscreening Agents / Q000737:chemistry / Q000494:pharmacology*; D014466:Ultraviolet Rays
C514808:1-(4-fluorphenyltioureido)-4-methyl-piperazine; D010879:Piperazines; D013473:Sunscreening Agents