[Diversity of actinomycetes associated with root-knot nematode and their potential for nematode control].
Twenty actinomycetes were isolated from root-knot nematode eggs and females collected from 11 plant root samples infested by Meloidogyne spp.. The isolates were assigned to the genera Streptomyces, Nocardia and Pseudonocardia respectively, based on analysis of morphological characteristics, cell-wall DAPs and 16S rRNA gene sequences. 80% of them were streptomycetes. Biocontrol potential of the isolates against Meloidogyne hapla was evaluated in liquid culture in vitro. The average percentages of egg parasitism, egg hatching, and juvenile mortality were 54.1, 40.4 and 26.2, respectively. Three Streptomyces strains and one Nocardia strain with high pathogenicity in vitro were selected to determine their ability to reduce tomato root galls in greenhouse. The results demonstrated good biocontrol efficacy (31.4%-56.4%) of the strains.
Luo
Hong-li
HL
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. luohl2005@eyou.com
Sun
Man-hong
MH
Xie
Jian-ping
JP
Liu
Zhi-heng
ZH
Huang
Ying
Y
D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D000818:Animals; D005260:Female; D018551:Solanum lycopersicum / Q000469:parasitology; D009348:Nematoda / Q000382:microbiology*; D009615:Nocardia / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D010063:Ovum / Q000382:microbiology; D010572:Pest Control, Biological / Q000379:methods*; D018517:Plant Roots / Q000469:parasitology*; D012336:RNA, Ribosomal, 16S; D013302:Streptomyces / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D014431:Tylenchoidea / Q000382:microbiology
D012336:RNA, Ribosomal, 16S