Temperature adaptation of Bacillus subtilis by chromosomal groEL replacement.
We investigated a temperature adaptation of Bacillus subtilis 168 in which chromosomal groEL was replaced with a psychrophilic groEL. This strain can grow at 50 degrees C but not at 51 degrees C, a temperature at which wild-type B. subtilis can grow. Using in vivo random mutagenesis by the B. subtilis mutator strain (mutS, mutM, mutY), two thermo-adaptants were isolated from the groEL substituted strain at 52 degrees C. They contained novel amino acid alterations in their ATP binding motif (T93I) and the inter-monomer contact (R285H) region of GroEL. These results suggest that GroEL participates in bacterial temperature adaptation.
Endo
Ayako
A
Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University, Japan.
Sasaki
Mayumi
M
Maruyama
Akihiko
A
Kurusu
Yasurou
Y
D000222:Adaptation, Physiological / Q000235:genetics*; D000255:Adenosine Triphosphate / Q000378:metabolism; D019943:Amino Acid Substitution; D001412:Bacillus subtilis / Q000235:genetics / Q000502:physiology*; D001665:Binding Sites / Q000235:genetics; D018834:Chaperonin 60 / Q000235:genetics / Q000502:physiology*; D016296:Mutagenesis; D012641:Selection, Genetic; D013696:Temperature
D018834:Chaperonin 60; D000255:Adenosine Triphosphate