Biomarkers in Diagnosis of pancreatic carcinoma in fine-needle aspirates.
This study was undertaken to determine whether recently identified proteins could be translated to clinical practice as markers to distinguish pancreatic adenocarcinoma from chronic pancreatitis on fine-needle aspirate (FNA) samples. Resected pancreatic tissue sections (n = 40) and FNA samples (n = 65) were stained for clusterin-beta, MUC4, survivin, and mesothelin. For each biomarker, the staining patterns in adenocarcinoma and in reactive ductal epithelium were evaluated and compared. Clusterin-beta stained reactive ductal epithelium significantly more frequently than pancreatic adenocarcinoma (P < .001). In comparison, MUC4 and mesothelin were expressed more frequently in pancreatic adenocarcinoma on tissue sections. Positive staining for MUC4 (91% vs 0%; P < .001) and mesothelin (62% vs 0%; P = .01) and absence of staining for clusterin-beta (90% vs 7%; P < .001) were noted significantly more frequently in adenocarcinoma cells than in reactive cells in FNA samples. Clusterin-beta and MUC4 can help distinguish reactive ductal epithelial cells from the cells of pancreatic adenocarcinoma in FNA samples.
Jhala
Nirag
N
Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35249, USA.
Jhala
Darshana
D
Vickers
Selwyn M
SM
Eltoum
Isam
I
Batra
Surinder K
SK
Manne
Upender
U
Eloubeidi
Mohamad
M
Jones
Jennifer J
JJ
Grizzle
William E
WE
D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology* / Q000601:surgery; D014408:Biomarkers, Tumor / Q000032:analysis; D044963:Biopsy, Fine-Needle / Q000379:methods*; D051152:Clusterin / Q000032:analysis; D003937:Diagnosis, Differential; D004847:Epithelial Cells / Q000737:chemistry / Q000473:pathology; D058851:GPI-Linked Proteins; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008562:Membrane Glycoproteins / Q000032:analysis; D000090204:Mesothelin; D008869:Microtubule-Associated Proteins / Q000032:analysis; D055264:Mucin-4; D009077:Mucins / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis; D010183:Pancreatic Ducts / Q000737:chemistry / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000601:surgery; D010195:Pancreatitis / Q000473:pathology; D012107:Research Design; D000077022:Survivin
C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; C495242:CLU protein, human; D051152:Clusterin; D058851:GPI-Linked Proteins; D051034:Inhibitor of Apoptosis Proteins; C524658:MUC4 protein, human; D008562:Membrane Glycoproteins; D008869:Microtubule-Associated Proteins; D055264:Mucin-4; D009077:Mucins; D009363:Neoplasm Proteins; D000077022:Survivin; D000090204:Mesothelin