Reverse line probe assay for the rapid detection of rifampicin resistance in Mycobacterium leprae.
Mutations in the rpoB gene of 40 biopsy isolates of Mycobacterium leprae were analyzed by reverse hybridization-based line probe assay after PCR, and nine distinct single-nucleotide substitutions were found. Among them, a 3-nucleotide substitution was found in two, and 2-nucleotide substitutions were found in seven isolates. This is a new finding of multiple mutations in a single point of the rpoB gene for rifampicin resistance. This investigation demonstrates that the pattern of mutations in the rpoB gene for rifampicin resistance in Nepal involves more variety.
Sapkota
Bishwa Raj
BR
Leprosy Mission Nepal, Mycobacterial Research Laboratory, Anandaban Hospital, Kathmandu, Nepal.
Ranjit
Chaman
C
Macdonald
Murdo
M
D001681:Biological Assay / Q000379:methods; D001706:Biopsy; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000187:drug effects; D006801:Humans; D007917:Leprostatic Agents / Q000494:pharmacology*; D007918:Leprosy / Q000188:drug therapy* / Q000382:microbiology; D009166:Mycobacterium leprae / Q000187:drug effects* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012293:Rifampin / Q000494:pharmacology*
D007917:Leprostatic Agents; D012293:Rifampin