Environmental temperature changes uptake rate and bioconcentration factors of bisphenol a in tadpoles of Rana temporaria.
Toxicokinetics of radiolabeled (14C) bisphenol A was studied in the common frog (Rana temporaria) at two experimental temperatures (7 and 19 degrees C). The growth rate of the tadpoles during the 96-h experiment was very slow at 7 degrees C, but the weight of tadpoles almost tripled at 19 degrees C. At all tested exposure concentrations (0.2, 1.5, 10, and 100 microg/L), conditional uptake rate constants (ku) were 69 to 82%, and elimination rates (ke) 79 to 90% lower, at 7 degrees C than at 19 degrees C. On the contrary, bioconcentration factors (BCFs) were higher at 7 degrees C than at 19 degrees C. Total accumulated bisphenol A per individual was higher at 19 degrees C, which is in agreement with higher k, at 19 degrees C. Exposure concentrations did not have any constant effect on BCFs at the two temperatures. The results of the current experiment suggest that higher temperature increases uptake and total amount of chemical in frog tadpoles but does not necessarily lead to higher BCFs. High temperature may have increased the growth rate more than the uptake rate, resulting in a net dilution of bisphenol A in tadpole tissues. The observed difference in BCFs also could be a result of temperature-induced changes in allometric relationships (increased surface area to volume ratio) and/or more effective elimination in more developed tadpoles at high temperature.
Honkanen
Jani O
JO
Laboratory of Aquatic Ecology and Ecotoxicology, Department of Biology, University of Joensuu, P.O. Box 111, FI-80101 Joensuu, Finland. jani.honkanen@joensuu.fi
Kukkonen
Jussi V K
JV
D000818:Animals; D001559:Benzhydryl Compounds; D007814:Larva / Q000254:growth & development / Q000378:metabolism*; D010636:Phenols / Q000493:pharmacokinetics*; D011896:Rana temporaria / Q000254:growth & development*; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds