In situ studies on the distribution patterns and dynamics of microcystins in a biomanipulation fish--bighead carp (Aristichthys nobilis).
The distribution and dynamics of microcystins in various organs of the phytoplanktivorous bighead carp were studied monthly in Lake Taihu, which is dominated by toxic cyanobacteria. There was a good agreement between LC-MS and HPLC-UV determinations. Average recoveries of spiked fish samples were 63% for MC-RR and 71% for MC-LR. The highest MC contents in intestine, liver, kidney and spleen were 85.67, 2.83, 1.70 and 1.57 microg g-1 DW, respectively. MCs were much higher in mid-gut walls (1.22 microg g-1 DW) than in hind- and fore-gut walls (0.31 and 0.18 microg g-1 DW, respectively), suggesting the importance of mid-gut wall as major site for MC absorption. A cysteine conjugate of MC-LR was detected frequently in kidney. Among the muscle samples analyzed, 25% were above the provisional tolerable daily intake level by WHO. Bighead is strongly resistant to microcystins and can be used as biomanipulation fish to counteract cyanotoxin contamination in eutrophic waters.
Chen
Jun
J
Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Donghu South Road 7, Wuhan, Hubei 430072, PR China.
Xie
Ping
P
Zhang
Dawen
D
Lei
Hehua
H
D000818:Animals; D001673:Biodegradation, Environmental; D001822:Body Burden; D002347:Carps / Q000378:metabolism*; D002681:China; D000458:Cyanobacteria; D004784:Environmental Monitoring / Q000379:methods; D005068:Eutrophication; D020387:Food Chain; D007422:Intestines / Q000737:chemistry; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D052998:Microcystins / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D010839:Phytoplankton; D012621:Seasons; D013154:Spleen / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
D052998:Microcystins; D014874:Water Pollutants, Chemical