title: Ex vivo HR-MAS MRS of human meningiomas: a comparison with in vivo 1H MR spectra.
abstract: We report on the magnetic resonance spectroscopy (MRS) characterisation of different human meningiomas. Three histological subtypes of meningiomas (meningothelial, fibrous and oncocytic) were analysed both through in vivo and ex vivo MRS experiments. The ex vivo high-resolution magic angle spinning (HR-MAS) investigations, permitting an accurate description of the metabolic profile, are very helpful for the assignment of the resonances in vivo of human meningiomas and for the validation of the quantification procedure of in vivo MR spectra. By using one- and two-dimensional experiments, we were able to identify several metabolites in different histological subtypes of meningiomas. Our spectroscopic data confirmed the presence of the typical metabolites of these benign neoplasms and, at the same time, that meningomas with different morphological characteristics have different metabolic profiles, particularly regarding macromolecules and lipids. The ex vivo spectra allowed a better understanding and interpretation of the in vivo MR spectra, showing that the HR-MAS MRS technique could be a complementary method to strongly support the in vivo MR spectroscopy and increase its clinical potentiality.
authors.affiliation: Dipartimento di Biochimica G. Moruzzi, Università di Bologna, 40126 Bologna, Italy.
authors.forename: Vitaliano
authors.initials: V
authors.lastname: Tugnoli
authors.forename: Luisa
authors.initials: L
authors.lastname: Schenetti
authors.forename: Adele
authors.initials: A
authors.lastname: Mucci
authors.forename: Francesca
authors.initials: F
authors.lastname: Parenti
authors.forename: Rita
authors.initials: R
authors.lastname: Cagnoli
authors.forename: Valeria
authors.initials: V
authors.lastname: Righi
authors.forename: Andrea
authors.initials: A
authors.lastname: Trinchero
authors.forename: Luca
authors.initials: L
authors.lastname: Nocetti
authors.forename: Cristian
authors.initials: C
authors.lastname: Toraci
authors.forename: Luciano
authors.initials: L
authors.lastname: Mavilla
authors.forename: Gianpaolo
authors.initials: G
authors.lastname: Trentini
authors.forename: Elena
authors.initials: E
authors.lastname: Zunarelli
authors.forename: M Raffaella
authors.initials: MR
authors.lastname: Tosi
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008577:Meningeal Neoplasms / Q000175:diagnosis* / Q000473:pathology; D008579:Meningioma / Q000175:diagnosis* / Q000473:pathology; D008875:Middle Aged; D013057:Spectrum Analysis
chemical_list: D014408:Biomarkers, Tumor