title: [Species identification by multiplex amplifying mtDNA-HV I, HV II and cytb regions].
abstract: OBJECTIVE
  To evaluate the method for species identification by multiplex amplification of mtDNA-HV I, HV II and cytb regions.
  
  
  METHODS
  The bloodstains or muscular tissues of human and 16 kinds of animals were collected. DNA were quantified after extraction. Three pairs of primers were mixed in the same reaction and the PCR amplification products were analyzed by PAGE and silver staining.
  
  
  RESULTS
  The presence of three bands (278 bp, 358 bp, 425 bp) indicated the samples were from human, while only one band indicated nonhuman origin. The position of the animal's only band is different from the band (358 bp) of human.
  
  
  CONCLUSIONS
  The method of species identification by multiplex amplifying mtDNA-HV I, HV II and cytb regions is simple and sensitive. It is of use for forensic identification.
authors.affiliation: Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Wang
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Yang
authors.forename: Wei-bo
authors.initials: WB
authors.lastname: Liang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002415:Cats; D002417:Cattle; D045303:Cytochromes b / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D004285:Dogs; D005554:Forensic Medicine; D006801:Humans; D051379:Mice; D021141:Nucleic Acid Amplification Techniques; D011817:Rabbits; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D045303:Cytochromes b