Toward intelligent nanosize bioreactors: a pH-switchable, channel-equipped, functional polymer nanocontainer.
To develop an intelligent sensor-effector functionality on the nanoscale, a pH-switchable, controlled nanoreactor based on amphiphilic copolymer membranes was built. The nanovesicles were equipped with bacterial transmembrane ompF pore proteins and the pH-sensitive enzyme acid phosphatase, resulting in a switchable substrate processing at pH 4-6.5. Ideal pH and substrate concentrations for the reaction were determined experimentally. In future, the reactor might be used for self-regulating targeted diagnostic and therapeutic applications in medicine.
Broz
Pavel
P
Medical Intensive Care Unit, University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland.
Driamov
Sergey
S
Ziegler
Joerg
J
Ben-Haim
Nadav
N
Marsch
Stephan
S
Meier
Wolfgang
W
Hunziker
Patrick
P
D019149:Bioreactors; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006863:Hydrogen-Ion Concentration; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D011108:Polymers / Q000737:chemistry*; D018272:Porins / Q000737:chemistry*
C109237:OmpF protein; D011108:Polymers; D018272:Porins