title: Transcriptional analysis between two wheat near-isogenic lines contrasting in aluminum tolerance under aluminum stress.
abstract: To understand the mechanisms of aluminum (Al) tolerance in wheat (Triticum aestivum L.), suppression subtractive hybridization (SSH) libraries were constructed from Al-stressed roots of two near-isogenic lines (NILs). A total of 1,065 putative genes from the SSH libraries was printed in a cDNA array. Relative expression levels of those genes were compared between two NILs at seven time points of Al stress from 15 min to 7 days. Fifty-seven genes were differentially expressed for at least one time point of Al treatment. Among them, 28 genes including genes for aluminum-activated malate transporter-1, ent-kaurenoic acid oxidase-1, beta-glucosidase, lectin, histidine kinase, and phospoenolpyruvate carboxylase showed more abundant transcripts in Chisholm-T and therefore may facilitate Al tolerance. In addition, a set of genes related to senescence and starvation of nitrogen, iron, and sulfur, such as copper chaperone homolog, nitrogen regulatory gene-2, yellow stripe-1, and methylthioribose kinase, was highly expressed in Chisholm-S under Al stress. The results suggest that Al tolerance may be co-regulated by multiple genes with diverse functions, and those genes abundantly expressed in Chisholm-T may play important roles in enhancing Al tolerance. The down-regulated genes in Chisholm-S may repress root growth and restrict uptake of essential nutrient elements, and lead to root senescence.
authors.affiliation: College of Life Science, Guangzhou University, Guangzhou, 510006, China.
authors.forename: Peiguo
authors.initials: P
authors.lastname: Guo
authors.forename: Guihua
authors.initials: G
authors.lastname: Bai
authors.forename: Brett
authors.initials: B
authors.lastname: Carver
authors.forename: Ronghua
authors.initials: R
authors.lastname: Li
authors.forename: Amy
authors.initials: A
authors.lastname: Bernardo
authors.forename: Michael
authors.initials: M
authors.lastname: Baum
mesh_terms: D000535:Aluminum / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects; D018076:DNA, Complementary; D004351:Drug Resistance / Q000187:drug effects / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development*; D013045:Species Specificity; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D000535:Aluminum