Development of an oxygen-rich biosensor using enzymatic reaction.
This work reports a novel strategy for the development of an O2-rich biosensor. The principle is based on an enzymatic reaction between catalase and H2O2 to release O2, thus to increase the O2 amount in the enzyme matrix. This method improves the determination reliability by alleviating the O2 dependence.
Cui
Yue
Y
Department of Chemical Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong. kecuiyue@ust.hk
Barford
John P
JP
Renneberg
Reinhard
R
D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D002374:Catalase / Q000737:chemistry; D002417:Cattle; D004563:Electrochemistry / Q000379:methods; D004798:Enzymes / Q000737:chemistry*; D005465:Fluorocarbon Polymers / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry*; D011138:Polytetrafluoroethylene / Q000737:chemistry
D004798:Enzymes; D005465:Fluorocarbon Polymers; C040402:perfluorosulfonic acid; D011138:Polytetrafluoroethylene; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D002374:Catalase; D009584:Nitrogen; D010100:Oxygen