Protein analysis of honeys by fast protein liquid chromatography: application to differentiate floral and honeydew honeys.
Fast protein liquid chromatography on a Superdex 75 HR column has been applied to analyze the proteins of 29 honeys, 12 of floral origin and 17 from honeydew. The molecular masses were comprised between 13100 and 94000 Da. Seven peaks have been separated; four of them were present in all of the honeys, and three were only present in some honeys. Direct observation of the chromatograms of the floral and honeydew honeys did not reveal any information about their botanical origins. However, both types of honeys can be distinguished with the percentages of the areas of four of the seven chromatographic peaks obtained.
Iglesias
M Teresa
MT
Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario, IMIDRA, Finca El Encín, Apartado 127, 28800 Alcala de Henares, Madrid, Spain, and Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
Martín-Alvarez
Pedro J
PJ
Polo
M Carmen
MC
de Lorenzo
Cristina
C
Pueyo
Encarnación
E
D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D035264:Flowers; D006722:Honey / Q000032:analysis* / Q000145:classification*; D011058:Pollen / Q000737:chemistry; D011506:Proteins / Q000032:analysis*
D000596:Amino Acids; D011506:Proteins