Establishment of immortalized dental follicle cells for generating periodontal ligament in vivo.
The dental follicle is a mesenchymal tissue that surrounds the developing tooth germ. During tooth root formation, periodontal components, viz., cementum, periodontal ligament (PDL), and alveolar bone, are created by dental follicle progenitors. Here, we report the presence of PDL progenitors in mouse dental follicle (MDF) cells. MDF cells were obtained from mouse incisor tooth germs and immortalized by the expression of a mutant human papilloma virus type 16 E6 gene lacking the PDZ-domain-binding motif. MDF cells expressing the mutant E6 gene (MDF( E6-EGFP ) cells) had an extended life span, beyond 150 population doublings (PD). In contrast, normal MDF cells failed to proliferate beyond 10 PD. MDF( E6-EGFP ) cells expressed tendon/ligament phenotype-related genes such as Scleraxis (Scx), growth and differentiation factor-5, EphA4, Six-1, and type I collagen. In addition, the expression of periostin was observed. To elucidate the differentiation capacity of MDF( E6-EGFP ) cells in vivo, the cells were transplanted into severe combined immunodeficiency mice. At 4 weeks, MDF( E6-EGFP ) cell transplants had the capacity to generate a PDL-like tissue that expressed periostin, Scx, and type XII collagen and the fibrillar assembly of type I collagen. Our findings suggest that MDF( E6-EGFP ) cells can act as PDL progenitors, and that these cells may be a useful research tool for studying PDL formation and for developing regeneration therapies.
Yokoi
T
T
Department of Medicine, Division of Operative Dentistry and Endodontics, Kanagawa Dental College, Yokosuka, Kanagawa, Japan.
Saito
M
M
Kiyono
T
T
Iseki
S
S
Kosaka
K
K
Nishida
E
E
Tsubakimoto
T
T
Harada
H
H
Eto
K
K
Noguchi
T
T
Teranaka
T
T
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002461:Cell Line, Transformed; D024042:Collagen Type I / Q000235:genetics; D024082:Collagen Type XII / Q000235:genetics; D003795:Dental Sac / Q000166:cytology* / Q000378:metabolism; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D055428:Growth Differentiation Factor 5; D007180:Incisor / Q000166:cytology / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D016513:Mice, SCID; D009856:Oncogene Proteins, Viral / Q000235:genetics; D015675:Osteocalcin / Q000235:genetics; D053495:Osteopontin / Q000235:genetics; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics
D000469:Alkaline Phosphatase; D051792:Basic Helix-Loop-Helix Proteins; D019485:Bone Morphogenetic Proteins; D015815:Cell Adhesion Molecules; D024042:Collagen Type I; D024082:Collagen Type XII; D049452:Green Fluorescent Proteins; D055428:Growth Differentiation Factor 5; D009856:Oncogene Proteins, Viral; D015675:Osteocalcin; D053495:Osteopontin; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C068651:E6 protein, Human papillomavirus type 16; C525893:GDF5 protein, human; C525894:Gdf5 protein, mouse; C082838:Postn protein, mouse; C503815:Scx protein, mouse; C485184:enhanced green fluorescent protein