A universal stress protein of Porphyromonas gingivalis is involved in stress responses and biofilm formation.
Porphyromonas gingivalis is recognized as one of the major periodontal pathogens in subgingival plaque, which is implicated in the progression of chronic periodontal disease. We analyzed the role of upsA in P. gingivalis 381 and its uspA-deficient mutant CW301 under various stress conditions. In general, the uspA mutant was less tolerant to a variety of environmental stresses relative to the parental strain. In addition, gene expression of uspA is upregulated during biofilm formation. Biofilm formation of the uspA mutant was also less than that of strain 381. In conclusion, the uspA gene affecting the stress responses of P. gingivalis is required for optimal biofilm formation.
Chen
Wen
W
Department of Oral Biology, School of Dental Medicine, University at Buffalo, State University of New York, Buffalo, New York, NY 14214-3092, USA.
Honma
Kiyonobu
K
Sharma
Ashu
A
Kuramitsu
Howard K
HK
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing; D006360:Heat-Shock Proteins / Q000235:genetics / Q000502:physiology*; D006358:Hot Temperature; D008826:Microbial Sensitivity Tests; D018384:Oxidative Stress; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013752:Tetracycline / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; C078564:universal stress protein A, Bacteria; D013752:Tetracycline