title: Effects of perillyl alcohol and heat shock treatment in gene expression of human lung adenocarcinoma cell line A549.
abstract: The K-Ras protein is found mutated in 42.4% of lung adenocarcinoma cases, evidencing its importance as a chemotherapeutic target. The Ras protein becomes functional after farnesylation, a post-transduction modification, allowing its attachment to the cellular membrane permitting signal transduction. Perillyl alcohol (POH) has been shown to inhibit the farnesylation of small G-proteins such as Ras. HSP70, a protein known to appear after heat shock (HS), is found over expressed in lung cancer and modifies chemotherapeutic effects. In this work, the effect of POH and HS in the gene expression of human adenocarcinoma lung cells (A549) is studied. Cells incubated with POH followed by 42 degrees C HS presented a 20.7% cellular viability decrease compared to the ones kept at 37 degrees C. A different pattern synthesis was observed for each sequences of cell treatment. Independent of the heat treatment, the amount of HSP70 was decrease by POH without modification in the amount of p53. Here it is shown that HS modified the POH effects in the ERK activation pathway by altering the phosphorylation of p44/42 in human adenocarcinoma lung cells.
authors.affiliation: Molecular Oncology Laboratory University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro (HUCFF-UFRJ), Brazil.
authors.forename: Juliana de Saldanha da Gama
authors.initials: Jde S
authors.lastname: Fischer
authors.forename: Paulo Costa
authors.initials: PC
authors.lastname: Carvalho
authors.forename: Cerli Rocha
authors.initials: CR
authors.lastname: Gattass
authors.forename: Eduardo Marcos Machado
authors.initials: EM
authors.lastname: Paschoal
authors.forename: Marcelo Soares
authors.initials: MS
authors.lastname: da Mota e Silva
authors.forename: Maria da Gloria da Costa
authors.initials: Mda G
authors.lastname: Carvalho
mesh_terms: D000230:Adenocarcinoma / Q000628:therapy*; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D018869:Heat-Shock Response; D006358:Hot Temperature; D006801:Humans; D008175:Lung Neoplasms / Q000628:therapy*; D039821:Monoterpenes / Q000494:pharmacology*; D010766:Phosphorylation; D015398:Signal Transduction
chemical_list: D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D039821:Monoterpenes; C032208:perillyl alcohol