[Deterimination of schisandrin in Kadsura heteroclita (Roxb.) Craib by RP-HPLC].
OBJECTIVE
To develop a method of RP-HPLC for determination of schisandrin content in Kadsura heteroclita (Roxb.) Craib.


METHODS
RP-HPLC analysis was carried out by using Kromasil C18 column (5 microm, 250 mm x 4.6 mm) and methanol-water (65:35) as the mobile, and the detection wavelength was 250 nm.


RESULTS
The method was linear in the range of 0.08-0.06 nicrog (r = 0.9998), and the average recovery was 100.24%, with the RSD of 2.09% (n=5).


CONCLUSIONS
The method was simple, accurate, highly sensitive and reproducible. It may be used for the quantitative determination of schisandrin in extract of Kadsura heteroclita (Roxb.) Craib.
Weng
Wen
W
Jinan University, Guangzhou 510632, China.
Gao
Qin
Q
Li
Xiao-guang
XG
Luo
Huan-min
HM
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D034242:Cyclooctanes / Q000032:analysis* / Q000302:isolation & purification; D032135:Kadsura / Q000737:chemistry* / Q000145:classification; D017705:Lignans / Q000032:analysis* / Q000302:isolation & purification; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011083:Polycyclic Compounds / Q000032:analysis* / Q000302:isolation & purification; D011786:Quality Control; D015203:Reproducibility of Results
D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; C011105:schizandrin