Study of the ITS region in an atypical isolate and comparison with six species of Microsporum.
Microsporum species are a frequent cause of cutaneous mycoses in humans. Atypical strains of Microsporum can sometimes be difficult to identify with conventional methods. Recently, we have obtained a Microsporum isolate with atypical morphology and special nutritional requirements (Microsporum CHUS-126-02). As several molecular techniques have been developed for the identification of fungi, we analysed six Microsporum species (M. canis, M. gypseum, M. gallinae, M. nanum, M. ferrugineum and M. persicolor) in order to compare them with our isolate, by using polymerase chain reaction-restriction enzyme analysis (PCR-REA). We studied the nucleotide sequence of the internal transcribed spacer regions from the nuclear DNA encoding for the ribosomal domain. Digestion with MvaI and EcoRI endonucleases obtained specific patterns for M. gypseum, M. gallinae, M. nanum and M. persicolor. Microsporum canis, M. ferrugineum and Microsporum CHUS-126-02 yielded the same patterns. Based on these results and phenotypic criteria, we classified our atypical isolate as M. canis.
Leon-Mateos
A
A
Department of Dermatology, Faculty of Medicine, Santiago de Compostela, Spain. manuelpe@usc.es
Paredes-Suárez
C
C
Pereiro
M
M
Toribio
J
J
D000293:Adolescent; D000328:Adult; D004262:DNA Restriction Enzymes / Q000378:metabolism; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D003881:Dermatomycoses / Q000382:microbiology; D005260:Female; D006801:Humans; D008864:Microsporum / Q000235:genetics*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D000091402:Prohibitins
D021903:DNA, Ribosomal Spacer; C000717331:PHB2 protein, human; D000091402:Prohibitins; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI