Aerobic growth of Escherichia coli with 2,4,6-trinitrotoluene (TNT) as the sole nitrogen source and evidence of TNT denitration by whole cells and cell-free extracts.
Escherichia coli grew aerobically with 2,4,6-trinitrotoluene (TNT) as sole nitrogen source and caused TNT's partial denitration. This reaction was enhanced in nongrowing cell suspensions with 0.516 mol nitrite released per mol TNT. Cell extracts denitrated TNT in the presence of NAD(P)H. Isomers of amino-dimethyl-tetranitrobiphenyl were detected and confirmed with U-15N-labeled TNT.
Stenuit
Ben
B
Unit of Bioengineering, Department of Applied Chemistry and Bioindustries, Catholic University of Lovain, Place Croix du Sud 2/19, 1348 Louvain-la-Neuve, Belgium.
Eyers
Laurent
L
Rozenberg
Raoul
R
Habib-Jiwan
Jean-Louis
JL
Agathos
Spiros N
SN
D000332:Aerobiosis; D001673:Biodegradation, Environmental; D003470:Culture Media; D004926:Escherichia coli / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D007700:Kinetics; D009573:Nitrites / Q000378:metabolism; D009584:Nitrogen; D014303:Trinitrotoluene / Q000378:metabolism*
D003470:Culture Media; D009573:Nitrites; D014303:Trinitrotoluene; D009584:Nitrogen