Assessing the genetic diversity of the aldolase genes of Plasmodium falciparum and Plasmodium vivax and its potential effect on performance of aldolase-detecting rapid diagnostic tests.
Malaria-specific rapid diagnostic tests (RDTs) targeting aldolase show highly variable sensitivities. We assessed diversity in Plasmodium falciparum and P. vivax aldolases by sequencing the coding genes from parasites of various origins. The results show that aldolases are highly conserved, indicating that antigenic diversity is not a cause of variable RDT sensitivity.
Lee
Nelson
N
The Australian Centre for International and Tropical Health, Brisbane, Australia.
Baker
Joanne
J
Bell
David
D
McCarthy
James
J
Cheng
Qin
Q
D000446:Aldehyde-Lyases / Q000235:genetics*; D000818:Animals; D017124:Conserved Sequence / Q000235:genetics*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D008288:Malaria / Q000175:diagnosis* / Q000469:parasitology; D016778:Malaria, Falciparum / Q000175:diagnosis / Q000469:parasitology; D016780:Malaria, Vivax / Q000175:diagnosis / Q000469:parasitology; D025202:Molecular Diagnostic Techniques; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D010966:Plasmodium vivax / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D015800:Protozoan Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D015800:Protozoan Proteins; D000446:Aldehyde-Lyases