title: The molecular phylogeny of the genus Rhizopus based on rDNA sequences.
abstract: In order to establish the molecular phylogeny of the genus Rhizopus, three molecules of the ribosomal RNA-encoding DNA (rDNA), complete 18S, internal transcribed spacer (ITS)1-5.8S-ITS2, and 28S D1/D2 regions of all the species of the genus were sequenced. Phylogenetic trees showed three major clusters corresponding to the three groups in the current morphological taxonomy, microsporus-group, stolonifer-group, and R. oryzae. R. stolonifer var. lyococcos was clustered independently from the major clusters. R. schipperae clustered differently in all trees. Strains of R. sexualis had multiple ITS sequences. A. rouxii clustered with R. oryzae. These results indicate the possibility of molecular identification of species groups using rDNA sequencing. Reclassification of the genus might be appropriate.
authors.affiliation: Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Abe
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oda
authors.forename: Kozo
authors.initials: K
authors.lastname: Asano
authors.forename: Teruo
authors.initials: T
authors.lastname: Sone
mesh_terms: D001483:Base Sequence; D002965:Classification; D016000:Cluster Analysis; D004275:DNA, Ribosomal / Q000235:genetics*; D019143:Evolution, Molecular; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S; D012233:Rhizopus / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S