Blueberry (Vaccinium corymbosum L.).
Recent advances in plant biotechnology have led to a reliable and reproductive method for genetic transformation of blueberry. These efforts built on previous attempts at transient and stable transformation of blueberry that demonstrated the potential of Agrobacterium tumefaciens-mediated transformation, and as well, the difficulties of selecting and regenerating transgenic plants. As a prerequisite for successful stable transformation, efficient regeneration systems were required despite many reports on factors controlling shoot regeneration from leaf explants. The A. tumefaciens-mediated transformation protocol described in this chapter is based on combining efficient regeneration methods and the results of A. tumefaciens-mediated transient transformation studies to optimize selected parameters for gene transfer. The protocol has led to successful regeneration of transgenic plants of four commercially important highbush blueberry cultivars.
Song
Guo-Qing
GQ
Plant Transformation Center, Department of Horticulture, Michigan State University, East Lansing, MI, USA.
Sink
Kenneth C
KC
D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D029798:Blueberry Plants / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D003470:Culture Media; D005966:Glucuronidase / Q000032:analysis; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000254:growth & development; D016133:Polymerase Chain Reaction; D012038:Regeneration; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
D003470:Culture Media; D005966:Glucuronidase