Determination of poly(3-hydroxybutyrate) using a combination of enzyme-based biosensor and alkaline hydrolysis.
The combination of an enzyme-based biosensor and alkaline hydrolysis was developed for the measurement of poly(3-hydroxybutyrate) (PHB). The principle of the determination is based on that the alkaline condition converts PHB to produce its monomer, 3-hydroxybutyrate (3-HB), which generates a detectable current signal by an amperometric biosensor through coupled two-enzyme reactions on an electrode. This method takes less than 40 min, and results in a linear detection range of 0.5-110 mg L-1 PHB with a detection limit of 0.3 mg L-1 by the saturated production of 3-HB; it can also take less than 15 min and result in a linear detection range of 1.0-160 mg L-1 PHB with a detection limit of 0.5 mg L-1 by a part production of 3-HB. The method also shows simple operation and high reproducibility.
Cui
Yue
Y
Department of Chemical Engineering, Hong Kong University of Science and Technology, China. kecuiyue@ust.hk
Barford
John P
JP
Renneberg
Reinhard
R
D015374:Biosensing Techniques; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004798:Enzymes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D006885:Hydroxybutyrates / Q000032:analysis*; D008956:Models, Chemical; D011091:Polyesters / Q000032:analysis*; D013696:Temperature; D013997:Time Factors; D000094122:Polyhydroxybutyrates
D004798:Enzymes; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates