title: An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries.
abstract: The protein factor U2 snRNP Auxiliary Factor (U2AF) 65 is an essential component required for splicing and involved in the coupling of splicing and 3' end processing of vertebrate pre-mRNAs. Here we have addressed the mechanisms by which U2AF 65 stimulates pre-mRNA 3' end processing. We identify an arginine/serine-rich region of U2AF 65 that mediates an interaction with an RS-like alternating charge domain of the 59 kDa subunit of the human cleavage factor I (CF I(m)), an essential 3' processing factor that functions at an early step in the recognition of the 3' end processing signal. Tethered functional analysis shows that the U2AF 65/CF I(m) 59 interaction stimulates in vitro 3' end cleavage and polyadenylation. These results therefore uncover a direct role of the U2AF 65/CF I(m) 59 interaction in the functional coordination of splicing and 3' end processing.
authors.affiliation: INSERM U563, Toulouse, France.
authors.forename: Stefania
authors.initials: S
authors.lastname: Millevoi
authors.forename: Clarisse
authors.initials: C
authors.lastname: Loulergue
authors.forename: Sabine
authors.initials: S
authors.lastname: Dettwiler
authors.forename: Sarah Zeïneb
authors.initials: SZ
authors.lastname: Karaa
authors.forename: Walter
authors.initials: W
authors.lastname: Keller
authors.forename: Michael
authors.initials: M
authors.lastname: Antoniou
authors.forename: Stéphan
authors.initials: S
authors.lastname: Vagner
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics / Q000378:metabolism*; D002474:Cell-Free System / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D026723:Polyadenylation / Q000502:physiology*; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D012326:RNA Splicing / Q000502:physiology*; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D000072668:Splicing Factor U2AF; D020798:Two-Hybrid System Techniques; D039221:mRNA Cleavage and Polyadenylation Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D009687:Nuclear Proteins; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF; C000607494:U2AF2 protein, human; C450562:cleavage factor Im, human; D039221:mRNA Cleavage and Polyadenylation Factors