title: Evaluation of the acquired immune responses to Plasmodium vivax VIR variant antigens in individuals living in malaria-endemic areas of Brazil.
abstract: BACKGROUND
  The naturally-acquired immune response to Plasmodium vivax variant antigens (VIR) was evaluated in individuals exposed to malaria and living in different endemic areas for malaria in the north of Brazil.
  
  
  METHODS
  Seven recombinant proteins representing four vir subfamilies (A, B, C, and E) obtained from a single patient from the Amazon Region were expressed in Escherichia coli as soluble glutathione S-transferase fusion proteins. The different recombinant proteins were compared by ELISA with regard to the recognition by IgM, IgG, and IgG subclass of antibodies from 200 individuals with patent infection.
  
  
  RESULTS
  The frequency of individuals that presented antibodies anti-VIR (IgM plus IgG) during the infection was 49%. The frequencies of individuals that presented IgM or IgG antibodies anti-VIR were 29.6% or 26.0%, respectively. The prevalence of IgG antibodies against recombinant VIR proteins was significantly lower than the prevalence of antibodies against the recombinant proteins representing two surface antigens of merozoites of P. vivax: AMA-1 and MSP119 (57.0% and 90.5%, respectively). The cellular immune response to VIR antigens was evaluated by in vitro proliferative assays in mononuclear cells of the individuals recently exposed to P. vivax. No significant proliferative response to these antigens was observed when comparing malaria-exposed to non-exposed individuals.
  
  
  CONCLUSIONS
  This study provides evidence that there is a low frequency of individuals responding to each VIR antigens in endemic areas of Brazil. This fact may explain the host susceptibility to new episodes of the disease.
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av, Prof, Lineu Prestes, 580, 05508-900, São Paulo, SP, Brazil. trolivei@usp.br
authors.forename: Tatiane R
authors.initials: TR
authors.lastname: Oliveira
authors.forename: Carmen
authors.initials: C
authors.lastname: Fernandez-Becerra
authors.forename: Maria Carolina S
authors.initials: MC
authors.lastname: Jimenez
authors.forename: Hernando A
authors.initials: HA
authors.lastname: Del Portillo
authors.forename: Irene S
authors.initials: IS
authors.lastname: Soares
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology*; D001938:Brazil / Q000453:epidemiology; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D016780:Malaria, Vivax / Q000453:epidemiology* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010966:Plasmodium vivax / Q000235:genetics* / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M