title: Unusual cyclin D1 positive marginal zone lymphoma of mediastinum.
abstract: We report a case of 43-yr-old Caucasian female with an unusual, cyclin D1 positive marginal zone lymphoma (MZL) of mucosa-associated lymphoid tissue (MALT) type of the mediastinum. To date, only about 30 cases of this entity have been published. They occur mainly in Asian females with a history of coexisting autoimmune disease. To our knowledge, this is the first case of mediastinal MZL with cyclin D1 expression. In the span of 6 yr this patient's tumor recurred three times, was surgically treated, and initially diagnosed as paraganglioma. The diagnosis was based on histopathological examination only. Our final diagnosis of MZL was made by combined evaluation of histopathology (HP), immunohistochemistry (IH), flow cytometry (FCM), fluorescence in situ hybridization (FISH), and molecular biology studies. We found a positive cyclin D1 reaction by IH and cyclin D1 mRNA (CCND1) overexpression by reverse transcription polymerase chain reaction (RT-PCR). Very high cyclin D1 to beta-actin mRNA ratio in this case was comparable with the ratio, characteristic for mantle cell lymphoma (MCL). However, there was no translocation t(11;14) found by FISH and an immunophenotype by IH and FCM was consistent with MZL ruling out MCL diagnosis. In addition, our case differs from other, previously reported thymic MZL lymphoma cases by no autoimmune disease association, Caucasian origin, and the absence of the plasmacytic differentiation on both HP/IH.
authors.affiliation: Department of Pathology, Centre of Oncology, Maria Skłodowska-Curie Memorial Institute, Warsaw, Poland. grym@coi.waw.pl
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Rymkiewicz
authors.forename: Konrad
authors.initials: K
authors.lastname: Ptaszyński
authors.forename: Jan
authors.initials: J
authors.lastname: Walewski
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Błachnio
authors.forename: Pawel
authors.initials: P
authors.lastname: Swoboda
authors.forename: Monika
authors.initials: M
authors.lastname: Gos
authors.forename: Ewa
authors.initials: E
authors.lastname: Paszkiewicz-Kozik
authors.forename: Renata
authors.initials: R
authors.lastname: Woroniecka
authors.forename: Barbara
authors.initials: B
authors.lastname: Pieńkowska-Grela
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Czarnocka
authors.forename: Przemysław
authors.initials: P
authors.lastname: Janik
mesh_terms: D000328:Adult; D019938:Cyclin D1 / Q000096:biosynthesis*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D018442:Lymphoma, B-Cell, Marginal Zone / Q000378:metabolism*; D020522:Lymphoma, Mantle-Cell / Q000235:genetics / Q000473:pathology; D008479:Mediastinal Neoplasms / Q000378:metabolism*; D008954:Models, Biological; D014178:Translocation, Genetic
chemical_list: D019938:Cyclin D1