Lysozyme binding onto cat-anionic vesicles.
Mixing aqueous sodium dodecylsulfate with cetyltrimethylammonium bromide solutions in mole ratios close to (1.7/1.0) allows the formation of cat-anionic vesicles with an excess of negative charges on the outer surface. The vesicular dispersions are mixed with lysozyme, and interact electrostatically with the positive charges on the protein, forming lipo-plexes. Dielectric relaxation, zeta-potential, and light scattering indicate the occurrence of interactions between vesicles and the protein. According to CD, the vesicle-adsorbed protein retains its native conformation. Binding and surface saturation, inferred by dielectric relaxation and zeta-potential, fulfil a charge neutralisation stoichiometry. Adsorbed lysozyme promotes the vesicle clustering and is concomitant with the lipo-plexes flocculation. Above the charge neutralisation threshold, lysozyme in excess remains dispersed in molecular form. Attempts were made to determine in what conditions protein release from the vesicles occurs. Accordingly, the full neutralisation of sodium dodecylsulfate in excess by cetyltrimethylammonium bromide ensures the lipo-plexes break-up, the precipitation of the mixed surfactants and the protein release in native form.
Bonincontro
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CNISM-Department of Physics, University of Rome La Sapienza, P. le A. Moro 5, I-00185 Roma, Italy.
Spigone
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E
Ruiz Peña
M
M
Letizia
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La Mesa
C
C
D000818:Animals; D000838:Anions / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D002645:Chickens; D009113:Muramidase / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*
D000838:Anions; D002412:Cations; D002593:Cetrimonium Compounds; D012967:Sodium Dodecyl Sulfate; D009113:Muramidase; D000077286:Cetrimonium