title: Antigenicity of borrelial protein BBK32 fragments in early Lyme borreliosis.
abstract: Recombinantly produced borrelial BBK32 protein fragments originating from Borrelia burgdorferi sensu stricto, Borrelia garinii and Borrelia afzelii were evaluated as antigens in the serology of Lyme borreliosis (LB). In ELISA, a mid-portion hydrophilic fragment reacted with LB patient sera. Of the 23 patients with culture- or PCR-positive erythema migrans (EM), 43 % at diagnosis and 52 % at convalescence were positive for at least one Borrelia species-specific variant BBK32 fragment antigen. In parallel ELISAs with BBK32 whole proteins from the three borrelial subspecies as antigens, 17 % at diagnosis and 26 % at convalescence were positive. These results suggest that BBK32 protein fragments may improve the early IgG serology of LB compared to the BBK32 whole protein.
authors.affiliation: Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, FIN-00290 Helsinki, Finland.
authors.forename: Pekka
authors.initials: P
authors.lastname: Lahdenne
authors.affiliation: HUSLAB Laboratory Diagnostics, Helsinki University Central Hospital, Haartmaninkatu 3, FIN-00290 Helsinki, Finland.|Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, FIN-00014 Helsinki, Finland.
authors.forename: Heikki
authors.initials: H
authors.lastname: Sarvas
authors.affiliation: Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, FIN-00014 Helsinki, Finland.
authors.forename: Riikka
authors.initials: R
authors.lastname: Kajanus
authors.affiliation: Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, FIN-00014 Helsinki, Finland.
authors.forename: Miia
authors.initials: M
authors.lastname: Eholuoto
authors.affiliation: Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, FIN-00014 Helsinki, Finland.
authors.forename: Heidi
authors.initials: H
authors.lastname: Sillanpää
authors.affiliation: HUSLAB Laboratory Diagnostics, Helsinki University Central Hospital, Haartmaninkatu 3, FIN-00290 Helsinki, Finland.|Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, FIN-00014 Helsinki, Finland.
authors.forename: Ilkka
authors.initials: I
authors.lastname: Seppälä
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001898:Borrelia / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008193:Lyme Disease / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007074:Immunoglobulin G; D010446:Peptide Fragments; D011994:Recombinant Proteins; C106209:p35 antigen, Borrelia