title: Rapid and efficient enzyme encapsulation in a dendrimer silica nanocomposite.
abstract: We report the entrapment of horseradish peroxidase and quantitative encapsulation of glucose oxidase within silica nanoparticles by utilizing an amine-terminated dendritic template. Our improved strategy employs a water-soluble biomimetic template which is able to catalyze the condensation of Si(OH)(4) to silica nanoparticles while trapping an enzyme inside the mesoporous material. Kinetic analysis shows enzyme functionality to be mostly unchanged. Also, the role of pI and ionic strength within the encapsulation environment was found to strongly influence encapsulation. These results suggest that the electrostatic manipulation of a strong supramolecular silica-precipitating complex of enzyme and dendrimer has the potential of adding a vast array of chemical and biological activity to hybrid materials. [image: see text] Enzyme immobilization within a silica nanocomposite.
authors.affiliation: Department of Chemistry, Vanderbilt University, Station B 351822, Nashville, Tennessee 37235-1822, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Miller
authors.forename: Edmund D
authors.initials: ED
authors.lastname: Hong
authors.forename: David
authors.initials: D
authors.lastname: Wright
mesh_terms: D001672:Biocompatible Materials; D050091:Dendrimers / Q000737:chemistry; D004339:Drug Compounding / Q000379:methods*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000138:chemical synthesis / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D050091:Dendrimers; D004800:Enzymes, Immobilized; C104700:PAMAM Starburst; D011073:Polyamines; D012822:Silicon Dioxide