[Inhibition of serine/threonine kinase 15 gene expression induces apoptosis of gastric cancer cells].
OBJECTIVE
To observe the effect of inhibition of serine/threonine kinase15 (STK15) gene expression on apoptosis induction in gastric cancer cell line-MKN45 and discuss the role of STK15 in viability of gastric cancer cells.


METHODS
The STK15 expression was inhibited by chemically synthesized siRNA. The STK15 mRNA and protein level were respectively measured by real-time quantitative PCR and western blotting,the change of cell cycle distribution and apoptosis rate were detected by flow-cytometry, cell morphological change was observed by Hoechst staining,and pro-caspase 3 level was also detected by western blot.


RESULTS
After treatment by siRNA targeting STK15 after 48 h, STK15 mRNA and protein level decreased obviously. More MKN45 cells accumulated at G(2)/M phase (P< 0.05). The apoptosis rate of STK15 siRNA treated MKN45 cells was higher than that of control cells(P< 0.05) with the pro-caspase 3 level decreased.


CONCLUSIONS
Inhibition of STK15 gene expression may induce apoptosis in MKN45 cells through the pathway of caspase3. STK15 gene play a key role in proliferation and viability of MKN45 cells.
Lan
Bin
B
Department of Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai Institute of Digestive Surgery, Shanghai 200001, China.
Liu
Bing-ya
BY
Chen
Xue-hua
XH
Qu
Ying
Y
Zhang
Xiao-qing
XQ
Cai
Qu
Q
Dai
Qi-bao
QB
Zhang
Jian
J
Zhu
Zheng-Gang
ZG
D017209:Apoptosis; D064096:Aurora Kinase A; D064127:Aurora Kinases; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D034741:RNA, Small Interfering; D013274:Stomach Neoplasms
D034741:RNA, Small Interfering; C576917:AURKA protein, human; D064096:Aurora Kinase A; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases