title: Analysis of the dermatophyte Trichophyton rubrum expressed sequence tags.
abstract: BACKGROUND
  Dermatophytes are the primary causative agent of dermatophytoses, a disease that affects billions of individuals worldwide. Trichophyton rubrum is the most common of the superficial fungi. Although T. rubrum is a recognized pathogen for humans, little is known about how its transcriptional pattern is related to development of the fungus and establishment of disease. It is therefore necessary to identify genes whose expression is relevant to growth, metabolism and virulence of T. rubrum.
  
  
  RESULTS
  We generated 10 cDNA libraries covering nearly the entire growth phase and used them to isolate 11,085 unique expressed sequence tags (ESTs), including 3,816 contigs and 7,269 singletons. Comparisons with the GenBank non-redundant (NR) protein database revealed putative functions or matched homologs from other organisms for 7,764 (70%) of the ESTs. The remaining 3,321 (30%) of ESTs were only weakly similar or not similar to known sequences, suggesting that these ESTs represent novel genes.
  
  
  CONCLUSIONS
  The present data provide a comprehensive view of fungal physiological processes including metabolism, sexual and asexual growth cycles, signal transduction and pathogenic mechanisms.
authors.affiliation: State Key Lab for Molecular Virology and Genetic Engineering, Beijing 100176, China. linda_wing@yahoo.com
authors.forename: Lingling
authors.initials: L
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Wenchuan
authors.initials: W
authors.lastname: Leng
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Lu
authors.initials: L
authors.lastname: Yu
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Wenliang
authors.initials: W
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.forename: Ying
authors.initials: Y
authors.lastname: Xue
authors.forename: Yafang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xingye
authors.initials: X
authors.lastname: Xu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wan
authors.forename: Guohui
authors.initials: G
authors.lastname: Ding
authors.forename: Fudong
authors.initials: F
authors.lastname: Yu
authors.forename: Kang
authors.initials: K
authors.lastname: Tu
authors.forename: Yixue
authors.initials: Y
authors.lastname: Li
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
mesh_terms: D003883:Arthrodermataceae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D002473:Cell Wall / Q000235:genetics; D030541:Databases, Genetic; D020224:Expressed Sequence Tags / Q000737:chemistry*; D005800:Genes, Fungal / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D014249:Trichophyton / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D010447:Peptide Hydrolases