[Expression and localization of estrogen receptor ERalpha and the kinds of ERbeta mRNA on placenta].
OBJECTIVE
To investigate the kinds of ERa, ERbeta mRNA and the localization of ERa, ERbeta on normal human placenta.


METHODS
The expression and localization of ERa,ERbeta on normal human placenta were detected by reverse transcription-ploymerase chain reaction (RT-PCR) and immunhistochemistry (SP-assay).


RESULTS
There was mRNA expression of ERalpha and ERbeta on human term placenta, and ERbeta was identified with ERbeta5. The ERa immunoreactivity on human term placenta was in villous cytotrophoblastic nucleus, and that of ERbeta was in syncytiotrophoblastic cytoplasm.


CONCLUSIONS
The findings indicate that ERbeta5 is the kind of ERbeta on placenta. It is localizated in the cytoplasm of syncytiotrophoblast and is probably connected with the production of estrogen.
Luo
Dan
D
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Liu
Shu-yun
SY
Xing
Ai-yun
AY
D000328:Adult; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004967:Estrogens; D012333:RNA, Messenger; D011960:Receptors, Estrogen