Recovery of temperate Desulfovibrio vulgaris bacteriophage using a novel host strain.
A novel sulfate-reducing bacterium (strain DePue) closely related to Desulfovibrio vulgaris ssp. vulgaris strain Hildenborough was isolated from the sediment of a heavy-metal impacted lake using established techniques. Although few physiological differences between strains DePue and Hildenborough were observed, pulse-field gel electrophoresis (PFGE) revealed a significant genome reduction in strain DePue. Comparative whole-genome microarray and polymerase chain reaction analyses demonstrated that the absence of genes annotated in the Hildenborough genome as phage or phage-related contributed to the significant genome reduction in strain DePue. Two morphotypically distinct temperate bacteriophage from strain Hildenborough were recovered using strain DePue as a host for plaque isolation.
Walker
Christopher B
CB
Department of Civil & Environmental Engineering, University of Washington, Seattle, WA, USA.
Stolyar
Sergey S
SS
Pinel
Nic
N
Yen
Huei-Che B
HC
He
Zhili
Z
Zhou
Jizhong
J
Wall
Judy D
JD
Stahl
David A
DA
D001435:Bacteriophages / Q000254:growth & development / Q000302:isolation & purification*; D016969:Desulfovibrio vulgaris / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology / Q000821:virology*; D016521:Electrophoresis, Gel, Pulsed-Field; D019015:Geologic Sediments / Q000382:microbiology; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D010948:Viral Plaque Assay; D014871:Water Microbiology