[RP-HPLC fingerprint for quality assessment of Radix Angelicae dahuricae].
OBJECTIVE
To establish the RP-HPLC fingerprint analysis for the quality control of Radix Angelicae dahuricae.


METHODS
HPLC fingerprint analysis method of Radix Angelicae dahuricae was developed. Kromasil C18 column (250 mm x 4.6 mm ID, 5 microm) was used, with mixture of acetonitrile and water as mobile phase in a gradient mode. The flow rate was 1.0 mL x min(-1). The wavelength of measurement was 254 nm. Twenty-one batches of Radix Angelicae dahuricae were determined.


RESULTS
The 21 samples were classified as 4 clusters by cluster analysis and the 11 superior in producing area samples were confirmed to establish the mutual model. The samples' quality was assessed by Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004.


CONCLUSIONS
The method can be used to identify and evaluate the quality of Radix Angelicae dahuricae conveniently.
Wang
Ting-ting
TT
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Chen
Xiao-hui
XH
Hu
Qing-qing
QQ
Bi
Kai-shun
KS
D029969:Angelica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000592:standards; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D010936:Plant Extracts