title: Post-meiotic shifts in HSPA2/HSP70.2 chaperone activity during mouse spermatogenesis.
abstract: HSPA2 (formerly HSP70.2) is a testis-specific member of the HSP70 family known to play a critical role in the completion of meiosis during male germ cell differentiation. Although abundantly present in post-meiotic cells, its function during spermiogenesis remained obscure. Here, using a global proteomic approach to identify genome-organizing proteins in condensing spermatids, we discovered an unexpected role for HSPA2, which acquires new functions and becomes tightly associated with major spermatid DNA-packaging proteins, transition proteins 1 and 2. Hence, HSPA2 is identified here as the first transition protein chaperone, and these data shed a new light on the yet totally unknown process of genome-condensing structure assembly in spermatids.
authors.affiliation: INSERM, U309, Institut Albert Bonniot, F-38700 Grenoble, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Govin
authors.forename: Cécile
authors.initials: C
authors.lastname: Caron
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Escoffier
authors.forename: Myriam
authors.initials: M
authors.lastname: Ferro
authors.forename: Lauriane
authors.initials: L
authors.lastname: Kuhn
authors.forename: Sophie
authors.initials: S
authors.lastname: Rousseaux
authors.forename: Edward M
authors.initials: EM
authors.lastname: Eddy
authors.forename: Jérôme
authors.initials: J
authors.lastname: Garin
authors.forename: Saadi
authors.initials: S
authors.lastname: Khochbin
mesh_terms: D000818:Animals; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D042003:DNA Packaging; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008540:Meiosis; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding; D040901:Proteomics; D013087:Spermatids / Q000166:cytology / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; C490434:Hspa2 protein, mouse; D009687:Nuclear Proteins; C489505:Tnp2 protein, mouse; C053886:spermatid transition proteins