title: mBET3 is required for the organization of the TRAPP complexes.
abstract: Large tethering complexes mediate the initial interaction of a transport vesicle with its target membrane. There are two forms of the multi-subunit tethering complex called TRAPP (TRAPPI and TRAPPII) that tether transport vesicles in different trafficking steps. Understanding TRAPP complex assembly and the protein-protein interactions among the subunits is an important step in elucidating the function of this tether. Here we have used several different approaches to study the protein-protein interactions among the subunits of the TRAPP complexes in both mammalian cells and yeast. Our studies have revealed that the low molecular weight subunits of TRAPP form two subcomplexes in vitro. One subcomplex contains mammalian BET3 (mBET3), mTRS31 and mTRS20, while mBET5 and mTRS23 form a second subcomplex. Furthermore, mBET3 directly interacts with mBET5 in vitro. Our findings also suggest that the TRAPPII-specific subunit, yTrs120p (yeast Trs120p), binds to the periphery of the TRAPPII complex. Although the non-essential TRAPP subunit yTrs33p interacts with yBet3p, yTrs33p is not required for TRAPP complex assembly. Together our findings indicate that BET3 plays an important role in the organization of the TRAPP complexes in both mammalian cells and yeast.
authors.affiliation: Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06519, USA.
authors.forename: Shekar
authors.initials: S
authors.lastname: Menon
authors.forename: Huaqing
authors.initials: H
authors.lastname: Cai
authors.forename: Hongyan
authors.initials: H
authors.lastname: Lu
authors.forename: Gang
authors.initials: G
authors.lastname: Dong
authors.forename: Yiying
authors.initials: Y
authors.lastname: Cai
authors.forename: Karin
authors.initials: K
authors.lastname: Reinisch
authors.forename: Susan
authors.initials: S
authors.lastname: Ferro-Novick
mesh_terms: D000818:Animals; D001665:Binding Sites; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D033921:Vesicular Transport Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; C496933:Trappc3 protein, mouse; D033921:Vesicular Transport Proteins; C406327:transport protein particle, TRAPP