Horizontal transfer of tet(M) and erm(B) resistance plasmids from food strains of Lactobacillus plantarum to Enterococcus faecalis JH2-2 in the gastrointestinal tract of gnotobiotic rats.
Two wild-type strains of Lactobacillus plantarum previously isolated from fermented dry sausages were analysed for their ability to transfer antibiotic resistance plasmids in the gastrointestinal tract. For this purpose, we used gnotobiotic rats as an in vivo model. Rats were initially inoculated with the recipient Enterococcus faecalis JH2-2 at a concentration of 10(10) CFU mL(-1). After a week, either of the two donors L. plantarum DG 522 (harbouring a tet(M)-containing plasmid of c. 40 kb) or L. plantarum DG 507 [harbouring a tet(M)-containing plasmid of c. 10 kb and an erm(B)-containing plasmid of c. 8.5 kb] was introduced at concentrations in the range of 10(8)-10(10) CFU mL(-1). Two days after donor introduction, the first transconjugants (TCs) were detected in faecal samples. The detected numbers of tet(M)-TCs were comparable for the two donors. In both cases, this number increased to c. 5 x 10(2) CFU g(-1) faeces towards the end of the experiment. For erm(B)-TCs, the number was significantly higher and increased to c. 10(3) CFU g(-1) faeces. To our knowledge, this is the first study showing in vivo transfer of wild-type antibiotic resistance plasmids from L. plantarum to E. faecalis.
Jacobsen
Louise
L
Department of Microbiology and Risk Assessment, Danish Institute for Food and Veterinary Research, Søborg, Denmark.
Wilcks
Andrea
A
Hammer
Karin
K
Huys
Geert
G
Gevers
Dirk
D
Andersen
Sigrid Rita
SR
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D015139:Blotting, Southern; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology; D005260:Female; D022761:Gene Transfer, Horizontal; D005856:Germ-Free Life; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000235:genetics*; D008297:Male; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D051381:Rats; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000235:genetics
D001426:Bacterial Proteins; C083138:Tet M resistance protein, Bacteria; D004917:Erythromycin; D013752:Tetracycline