title: Development of novel immunoglobulin G (IgG), IgA, and IgM enzyme immunoassays based on recombinant Puumala and Dobrava hantavirus nucleocapsid proteins.
abstract: Human infections with Asian and European hantaviruses can result in hemorrhagic fever with renal syndromes of differing severities characterized by renal dysfunction and sometimes by pulmonary symptoms. For the serological detection of human infections by hantaviruses relevant for Europe, we developed monoclonal antibody capture immunoglobulin G (IgG) and IgA enzyme-linked immunosorbent assays (ELISAs) based on yeast-expressed nucleocapsid proteins of Puumala and Dobrava hantaviruses. Moreover, for diagnosis of acute infections, mu-capture IgM ELISAs were established with nucleocapsid proteins expressed in Drosophila melanogaster Schneider S2 cells. The cutoff values of the ELISAs were determined by investigation of up to 500 human anti-hantavirus-negative serum samples. The specificities of the Puumala and Dobrava virus-specific IgM, IgA, and IgG ELISAs were found to be 100%. The sensitivities of these ELISAs were determined to be 100% with panels of characterized anti-Puumala or anti-Dobrava virus-positive human serum samples. In most cases, Puumala and Dobrava virus infections could be differentiated by ELISA reactivity alone, i.e., endpoint titration with homologous and heterologous antigens.
authors.affiliation: Institute of Virology, Helmut-Ruska-Haus, Charité-Universitätsmedizin Berlin, Campus Charité Mitte, Charitéplatz 1, 10117 Berlin, Germany. helga.meisel@charite.de
authors.forename: Helga
authors.initials: H
authors.lastname: Meisel
authors.forename: Anne
authors.initials: A
authors.lastname: Wolbert
authors.forename: Ausra
authors.initials: A
authors.lastname: Razanskiene
authors.forename: Andreas
authors.initials: A
authors.lastname: Marg
authors.forename: Andris
authors.initials: A
authors.lastname: Kazaks
authors.forename: Kestutis
authors.initials: K
authors.lastname: Sasnauskas
authors.forename: Georg
authors.initials: G
authors.lastname: Pauli
authors.forename: Rainer
authors.initials: R
authors.lastname: Ulrich
authors.forename: Detlev H
authors.initials: DH
authors.lastname: Krüger
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006476:Orthohantavirus / Q000235:genetics / Q000276:immunology*; D018778:Hantavirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D029262:Puumala virus / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins