title: Non-radioactive and colorimetric quantification of monocyte adhesion to endothelial cells in early atherogenesis.
abstract: Monocyte adhesion to vascular endothelium is an initial step in atherogenesis. To quantify this, we incubated monocytes with cultured endothelial cells, and quantified the adhered live monocytes using a colorimetric assay. Endothelium activated with lipopolysaccharide attracted monocytes in a dose-dependent manner and the adhesion was attenuated with post-treatments with L-ascorbic acid (53%), alpha- (40%) and gamma-tocopherol (39%), resveratrol (39%), and Lithospermum erythrorhizon root extract (45%). This non-radioactive, colorimetric assay may be useful for screening anti-atherogenic compounds in early atherogenesis.
authors.affiliation: Division of Food Science, College of Life Science and Biotechnology, Institute of Biomedical Sciences and Safety, Korea University, Seoul, 136-713, Korea.
authors.forename: Hee Jin
authors.initials: HJ
authors.lastname: Jun
authors.forename: Mi Ja
authors.initials: MJ
authors.lastname: Chung
authors.forename: Sang Yeon
authors.initials: SY
authors.lastname: Kim
authors.forename: Ho Joung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Sung Joon
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D050197:Atherosclerosis; D002448:Cell Adhesion; D002478:Cells, Cultured; D018920:Coculture Techniques; D003124:Colorimetry / Q000295:instrumentation* / Q000379:methods; D019295:Computational Biology / Q000295:instrumentation* / Q000379:methods; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism; D029684:Lithospermum / Q000378:metabolism; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D018517:Plant Roots; D024502:alpha-Tocopherol / Q000737:chemistry; D024504:gamma-Tocopherol / Q000737:chemistry
chemical_list: D008070:Lipopolysaccharides; D024504:gamma-Tocopherol; D024502:alpha-Tocopherol