Delivery and mechanistic considerations for the production of knock-in mice by single-stranded oligonucleotide gene targeting.
Single-stranded oligodeoxynucleotide (ssODN) gene targeting may facilitate animal model creation and gene repair therapy. Lipofection of ssODN can introduce point mutations into target genes. However, typical efficiencies in mouse embryonic stem cells (ESC) are <10(-4), leaving corrections too rare to effectively identify. We developed ESC lines with an integrated mutant neomycin resistance gene (Tyr22Ter). After targeting with ssODN, repaired cells survive selection in G418. Correction efficiencies varied with different lipofection procedures, clonal lines, and ssODN designs, ranging from 1 to 100 corrections per million cells plated. Uptake studies using cell sorting of Cy5-labelled ssODN showed 40% of the corrections concentrated in the best transfected 22% of cells. Four different basepair mismatches were tested and results show that the base-specificity of the mismatch is critical. Dual mismatch ssODN also showed mismatch preferences. These ESC lines may facilitate development of improved ssODN targeting technologies for either animal production or ex vivo gene therapy.
Murphy
B R
BR
Department of Chemical and Biomolecular Engineering, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, USA.
Moayedpardazi
H S
HS
Gewirtz
A M
AM
Diamond
S L
SL
Pierce
E A
EA
D000818:Animals; D001483:Base Sequence; D002678:Chimera; D004260:DNA Repair; D004277:DNA, Single-Stranded; D004351:Drug Resistance / Q000235:genetics; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005260:Female; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering; D008081:Liposomes; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D008969:Molecular Sequence Data; D009355:Neomycin; D014162:Transfection / Q000379:methods
D004277:DNA, Single-Stranded; D008081:Liposomes; D009355:Neomycin