title: Recombinant protein Bd37 protected gerbils against heterologous challenges with isolates of Babesia divergens polymorphic for the bd37 gene.
abstract: The Bd37gene encoding for a glycosyl-phosphatidyl-inositol anchored protein of Babesia divergens displays genetic polymorphisms among isolates. Five major polymorphic groups (clades) were shown by PCR-RFLP among different B. divergens isolates. Each group has been characterized according to a reference Bd37 gene (Rouen87, W8843, Y5, 6303E and 1705B). Recombinant (GST fusion) protein (Bd37r) expressed from the Bd37 gene, was used as antigen in a saponin-based formulation and was able to protect gerbils, after 2 injections at low dose vaccine concentration (1 mug per dose), against a virulent challenge with the B. divergens Rouen87 isolate. In spite of polymorphism of Bd37 gene, Bd37r induced complete immunoprotection against challenges with each of the 5 reference isolate groups defined by PCR-RFLP.
authors.affiliation: Laboratoire Biologie Cellulaire and Moléculaire, ERT 1038 'Vaccination anti-parasitaire', UFR Pharmacie, Université Montpellier I, 15 Avenue Charles Flahault, B.P. 14491, 34093 Montpellier Cedex 5, France. kamel.hadj-kaddour@univ-montp1.fr
authors.forename: K
authors.initials: K
authors.lastname: Hadj-Kaddour
authors.forename: B
authors.initials: B
authors.lastname: Carcy
authors.forename: A
authors.initials: A
authors.lastname: Vallet
authors.forename: S
authors.initials: S
authors.lastname: Randazzo
authors.forename: S
authors.initials: S
authors.lastname: Delbecq
authors.forename: J
authors.initials: J
authors.lastname: Kleuskens
authors.forename: T
authors.initials: T
authors.lastname: Schetters
authors.forename: A
authors.initials: A
authors.lastname: Gorenflot
authors.forename: E
authors.initials: E
authors.lastname: Precigout
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology*; D001403:Babesia / Q000235:genetics*; D001483:Base Sequence; D004306:Dose-Response Relationship, Immunologic; D005849:Gerbillinae; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D016052:Protozoan Vaccines; D012376:Rodent Diseases / Q000469:parasitology* / Q000517:prevention & control; D016415:Sequence Alignment
chemical_list: D000953:Antigens, Protozoan; C488800:Bd37 exoantigen, Babesia divergens; D016052:Protozoan Vaccines