Proteins induced by cadmium in soybean cells.
The cadmium (Cd)-induced changes in protein pattern and identification of metal-stimulated polypeptides were analyzed in soybean cell suspension culture. The cell cultures were treated with various concentrations of Cd(2+) (3-10microM) for 24, 48 and 72h. The synthesis of [(35)S]-labeled proteins and their accumulation were analyzed by SDS-PAGE, whereas the identification of selected protein bands was performed by mass spectrometry. It is shown that Cd induced the appearance of the following proteins in soybean cells: superoxide dismutase, histone H2B, chalcone synthase and glutathione transferase.
Sobkowiak
Robert
R
Department of Cell Biology, Faculty of Biology, Adam Mickiewicz University, ul. Fredry 10, 61-701 Poznań, Poland. robsob@amu.edu.pl
Deckert
Joanna
J
D000217:Acyltransferases / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D040901:Proteomics; D013025:Glycine max / Q000737:chemistry / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
D006657:Histones; D010940:Plant Proteins; D002104:Cadmium; D013482:Superoxide Dismutase; D000217:Acyltransferases; C020141:flavanone synthetase; D005982:Glutathione Transferase