Rapid screening of topoisomerase gene mutations by a novel melting curve analysis method for early warning of fluoroquinolone-resistant Streptococcus pneumoniae emergence.
We developed a real-time PCR assay combined with melting curve analysis for rapidly genotyping quinolone resistance-determining regions (QRDR) of topoisomerase genes in Streptococcus pneumoniae. This assay was not only accurate for the screening of fluoroquinolone (FQ) resistance but also relevant as an early warning system for detecting preexisting single QRDR mutations.
Fukushima
Kazuko Y
KY
Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki City 852-8501, Japan.
Hirakata
Yoichi
Y
Sugahara
Kazuyuki
K
Yanagihara
Katsunori
K
Kondo
Akira
A
Kohno
Shigeru
S
Kamihira
Shimeru
S
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D026942:DNA Topoisomerases / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D024841:Fluoroquinolones / Q000494:pharmacology*; D005838:Genotype; D006801:Humans; D009154:Mutation; D011008:Pneumococcal Infections / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D044366:Transition Temperature
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D026942:DNA Topoisomerases