title: Acidic extracellular proteases from microrganisms of fairly acidic niche.
abstract: The protein population secreted from three wine-contaminant microorganisms was studied by two-dimensional electrophoresis and screened for proteases. Proteolytic enzymes were electrophoretically purified and their activity, optimum pH and temperature determined. The protease of Acetobacter aceti maintained its activity over the range of pHs 3.0-5.0, thus being of potential biotechnological interest.
authors.affiliation: Department of Science and Technology, University of Verona, Strada le Grazie 15, 37134 Verona, Italy. alessandra.bossi@univr.it
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bossi
authors.forename: Luca
authors.initials: L
authors.lastname: Bonizzato
authors.forename: Giacomo
authors.initials: G
authors.lastname: Zapparoli
mesh_terms: D000091:Acetobacter / Q000201:enzymology*; D001203:Ascomycota / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D010447:Peptide Hydrolases