title: Human neutrophil peptides 1, 2 and 3 are biochemical markers for metastatic colorectal cancer.
abstract: Colorectal cancer (CRC) patients have increased levels of human neutrophil peptides 1-3 (HNP1-3) in tumour tissue and plasma. The aim is to study whether the amount of HNP1-3 in tumour and plasma from CRC patients correlate with Dukes' stages A-D. The amount of HNP1-3 in tumour tissue, normal colonic mucosa, and plasma was determined with mass spectrometry. Plasma levels of HNP1-3 were determined with enzyme-linked immuno-sorbent assay (ELISA). The amount of HNP1-3 determined with mass spectrometry was increased in tumours compared to normal colonic tissue in CRC patients in Dukes' stage A-D, whereas HNP1-3 in plasma was only elevated in Dukes' stage D compared to healthy individuals. HNP1-3 plasma concentration determined with ELISA was increased in Dukes' stages C and D, but not in A and B. It is concluded that HNP1-3 is a potential marker for prognostic assessment, surveillance of patients, and monitoring chemotherapy in CRC patients with advanced disease.
authors.affiliation: Department of Clinical Biochemistry, Glostrup Hospital, 2600 Glostrup, Denmark. jakalb01@glostruphosp.kbhamt.dk <jakalb01@glostruphosp.kbhamt.dk>
authors.forename: Jakob
authors.initials: J
authors.lastname: Albrethsen
authors.forename: Christian H
authors.initials: CH
authors.lastname: Møller
authors.forename: Jesper
authors.initials: J
authors.lastname: Olsen
authors.forename: Hans
authors.initials: H
authors.lastname: Raskov
authors.forename: Steen
authors.initials: S
authors.lastname: Gammeltoft
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D023084:alpha-Defensins / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D023084:alpha-Defensins; C406413:human neutrophil peptide 1; C406416:human neutrophil peptide 2; C406427:human neutrophil peptide 3