Sensitive and rapid electrochemical bioassay of glycosidase activity.
Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.
Tangkuaram
Tanin
T
The Biodesign Institute and Fulton School of Engineering, Arizona State University, Tempe, AZ 85287-5801, USA.
Gerlach
Jared Q
JQ
Xiang
Yun
Y
Kawde
Abdel-Nasser
AN
Dai
Zong
Z
Bhavanandan
Veer P
VP
La Belle
Jeffrey T
JT
Veerasai
Waret
W
Joshi
Lokesh
L
Wang
Joseph
J
D001419:Bacteria / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008362:Mannosides / Q000378:metabolism; D009596:Nitrophenols / Q000032:analysis / Q000378:metabolism
D008362:Mannosides; D009596:Nitrophenols; C036119:4-nitrophenyl-beta-D-mannopyranoside; D006026:Glycoside Hydrolases