title: Three-dimensional collagen fiber remodeling by mesenchymal stem cells requires the integrin-matrix interaction.
abstract: Tissue engineering aiming to repair or regenerate damaged tissues necessitates fabricating three-dimensional biomaterial scaffolds with controlled porosity for delivering cells. To facilitate cell distribution, a strategy using stem cell-based fabrication of biomaterials was tested in type II collagen fibers. Human mesenchymal stem cells when delivered in type II collagen assembled and reorganized these matrices and differentiated into spherical chondrocytes with the synthesis of cartilage proteins. The cell-mediated assembly and reorganization of collagen fibers was not limitless and only restricted to an appropriate ratio of cell number and collagen amount. The blocking of alpha2 or beta1-integrin function with specific antibodies significantly impeded the collagen-assembly effects. In vitro chondrogenesis or in vivo cartilage formation of human mesenchymal stem cells was also dependent on the interactions between cells and surrounding matrices. This method for three-dimensional fabricating collagen fibers may generally be applied to other biomaterials, when combined with surface modification or ligand addition for cell adhesion.
authors.affiliation: Stem Cell Laboratory, Medical Research and Education, Veterans General Hospital-Taipei, 201, Sec. 2, Shih-Pai Road, 112 Taipei, Taiwan, Republic of China.
authors.forename: Ching-Fang
authors.initials: CF
authors.lastname: Chang
authors.forename: Ming-Wei
authors.initials: MW
authors.lastname: Lee
authors.forename: Pei-Yin
authors.initials: PY
authors.lastname: Kuo
authors.forename: Yng-Jiin
authors.initials: YJ
authors.lastname: Wang
authors.forename: Yi-Hsien
authors.initials: YH
authors.lastname: Tu
authors.forename: Shih-Chieh
authors.initials: SC
authors.lastname: Hung
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D019902:Chondrocytes / Q000166:cytology; D020219:Chondrogenesis; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D011485:Protein Binding; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D016023:Integrins; D003094:Collagen