Determination of ganciclovir in different matrices from solid organ transplanted patients treated with a wide range of concomitant drugs.
The aim of the present study was to develop a time-efficient chromatographic method for the analysis of therapeutic concentrations of ganciclovir (GCV) in plasma, urine as well as dialysate (from continuous renal replacement therapy) from solid organ transplant recipient treated with either GCV or its prodrug valganciclovir (VGCV) in combination with a wide variety of other concomitant drugs. Sample preparation was performed by reversed phase solid phase extraction and was followed by separation of the analytes on a reversed phase column using isocratic elution with a mobile phase consisting of acetonitrile-a counter ion (50 mM 1-heptanesulfonic acid) in an aqueous sodium dihydrogen phosphate buffer (pH 2.1; 10 mM) (10:90 v/v) and a fluorescence detector. Validation of the method showed linearity within the concentration range of 0.1-40 microg/mL for plasma and 0.1-120 microg/mL for urine and dialysate (R(2)>0.99, n> or =5). Accuracy and precision (evaluated at 0.1, 5 and 40 microg/mL) were both satisfactory. The LLOQ was determined to be 0.1 microg/mL. The method was successfully applied on clinical samples from renal transplant recipients treated with VGCV in combination with a variety of usually used concomitant drugs for solid organ transplant recipients.
Asberg
Anders
A
Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Blindern, Oslo, Norway. anders.asberg@farmasi.uio.no
Hansen
Christin Nupen
CN
Reubsaet
Leon
L
D000998:Antiviral Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis; D015774:Ganciclovir / Q000032:analysis* / Q000097:blood / Q000652:urine; D007202:Indicators and Reagents; D016377:Organ Transplantation; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D000998:Antiviral Agents; D007202:Indicators and Reagents; D015774:Ganciclovir