Midgut glycosidases activities in monophagous larvae of Apollo butterfly, Parnassius apollo ssp. frankenbergeri.
Parnassius apollo (Lepidoptera, Papilionidae) declines on numerous localities all over Europe. Its local subspecies frankenbergeri, inhabiting the Pieniny Mts (southern Poland) and successfully recovered from extinction, is monophagous in larval stage. In natural conditions, it completes development on the orpine Sedum telephium ssp. maximum. Since proper quality and quantity of necessary nutritional compounds of the food plant ensure developmental success, the digestive processes in the insect midgut should reflect adaptation to a specific food source. The paper presents, for the first time, the activity of detected glycolytic enzymes in midgut tissue and liquid gut contents of the L4 and L5 instars of P. apollo larvae. alpha-Amylase plays the main role in utilization of carbohydrates, contrary to cellulase activity. Saccharase seems to be the main disaccharidase, and high activity of beta-glycosidase enables hydrolysis of the plant glycosides. Trehalase activity was unexpectedly low and comparable to those of cellobiase and lactase. alpha-Amylolytic and other glycolytic activities indicate that larvae utilize starch and other carbohydrate compounds as energy sources. Possible use of some plant allelochemicals as energy sources by Apollo larvae is discussed.
Nakonieczny
Mirosław
M
Department of Animal Physiology and Ecotoxicology, University of Silesia, Bankowa 9, PL 40-007 Katowice, Poland. nakon@us.edu.pl
Michalczyk
Katarzyna
K
Kedziorski
Andrzej
A
D000818:Animals; D002080:Butterflies / Q000254:growth & development*; D002480:Cellulase / Q000378:metabolism; D004064:Digestive System / Q000201:enzymology*; D005696:Galactosidases / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007700:Kinetics; D007814:Larva / Q000201:enzymology*; D000516:alpha-Amylases / Q000378:metabolism
D005696:Galactosidases; D005959:Glucosidases; D006026:Glycoside Hydrolases; D000516:alpha-Amylases; D002480:Cellulase