[Comparative research on the implantation of mouse blastocyst in the co-cultural system of malignant tumor Cells and receptive endometrium in vitro].
OBJECTIVE
Comparaticely to study biological behaviour variability of mouse embryo implantation in vitro between malignant tumor cells and receptive endometrium, on the basis of the co-culture of mouse blastocyst and malignant tumor cells in vitro.


METHODS
The light scope, scanning electron scope, the HE and immunohistochemical staining were applied to investigate and observe the blastocyst biology behavior in co-cultural system in vitro of mouse blastocyst-malignant cell and blastocyst-monolayer receptive endometrium.


RESULTS
There was no significant biological behaviors observed or discovered for mouse blastocysts to implant into co-cultural system with malignant tumor cells from different histologcial origins, and no significant rates obtained for hatchment, attachment and outgrowth of mouse blastocysts in the co-culture system (P > 0.05) of monolayer receptival endometrium cells and malignant tumor cells. There was no significant difference observed between the cell morphology changes of implanted in endometrium and MMP-9 expressed in trophoblastic cells.


CONCLUSIONS
That the mouse blastocyst cells has no biological behaviour difference happened between two co-cultural systems in vitro indicates it may be relevant with its high adaptation to environment and self assembly of the early life.
Ma
Fang
F
Department of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China.
Wang
Zhi-biao
ZB
Wang
Yuan
Y
Zhao
Jie
J
D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D010064:Embryo Implantation / Q000502:physiology*; D004717:Endometrium / Q000166:cytology* / Q000502:physiology; D005260:Female; D008113:Liver Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D010051:Ovarian Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured