Isolation and expansion of allogeneic myeloma-specific interferon-gamma producing T cells for adoptive immunotherapy.
Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4(+) and CD8(+) T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 x 106 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)-based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications.
Ocadlikova
Darina
D
Laboratory of Experimental Hematology and Cell Immunotherapy, Department of Clinical Hematology, University Hospital, Brno, Czech Republic. docadlikova@fnbrno.cz
Zahradova
L
L
Kovarova
L
L
Penka
M
M
Zaloudik
J
J
Buchler
T
T
Hajek
R
R
Michalek
J
J
D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D005452:Fluoresceins / Q000737:chemistry; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007371:Interferon-gamma / Q000378:metabolism*; D008280:Magnetics; D008495:Medical Oncology / Q000379:methods; D009101:Multiple Myeloma / Q000276:immunology*; D013388:Succinimides / Q000737:chemistry; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014184:Transplantation, Homologous
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D013388:Succinimides; D007371:Interferon-gamma