[Influence of blockade of costimulation on Th1/Th2 cytokines shift in unexplained early recurrent spontaneous].
OBJECTIVE
To assess the influence of blockade of costimulation on Th1/Th2 Cytokines shift in unexplained early recurrent spontaneous abortion (URSA).


METHODS
The levels of IL-2, IFN-gamma and IL-4 were determined by ELISA in the supernatant of cultured maternal peripheral blood mononuclear cells (PBMC) from 30 patients of URSA. Cytokine synthesis was induced by activation with antigens from a choriocarcinomer cell line of JEG-3, with CTLA4Ig at concentrations of 10 microg/mL and 1 microg/mL (experiment groups), or with IgG at the same concentrations (control groups).


RESULTS
The CTLA4Ig groups showed significantly higher levels of Th2 cytokines IL-4 (P < 0.05), lower levels of Th1 cytokines IL-2 (P < 0.05) and the same levels of IFN-gamma (P > 0.05), compared with the control groups of the same concentrations respectively. The same results were found between the experiment groups of different concentration.


CONCLUSIONS
CTLA4Ig could switch T cell responses from Th1 to Th2 in URSA by blockade of CD80/CD86 costimulation.
Fan
Wei
W
Department of Reproduction, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Li
Shang-wei
SW
Liu
Xi-ru
XR
D000026:Abortion, Habitual / Q000276:immunology*; D000328:Adult; D018122:B7-1 Antigen / Q000276:immunology; D051940:B7-2 Antigen / Q000276:immunology; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D007376:Interleukin-2 / Q000097:blood*; D015847:Interleukin-4 / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D011247:Pregnancy; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
D018122:B7-1 Antigen; D051940:B7-2 Antigen; D007376:Interleukin-2; D015847:Interleukin-4; D007371:Interferon-gamma