title: [A study on transfection of green fluorescence protein gene into human adipose stromal cells in vitro].
abstract: OBJECTIVE
  To make a comparison on the efficiency of two methods for transfecting Green Fluorescence Protein gene into human adipose tissue-derived stromal cells, and to study the biological properties and multipotential differentiation of gene-transfected cells.
  
  
  METHODS
  The human subcutaneous adipose tissue was obtained, digested with one volume of collagenase type I, and then cultured with BGJb medium. After subculture and expansion, the human adipose tissue-derived stromal cells infected with Ad-GFP or liposome were observed and analyzed with fluorescence microscopy and flow cytometry to assess transfection efficiency. The growth curve of transfected adipose tissue-derived stromal cells was protracted. The adipose tissue-derived storomal cells were induced to differentiate into osteoblasts, and non-transfected cells were set as control.
  
  
  RESULTS
  42.5% +/- 1.5 of the human adipose tissue-derived stomal cells infected with Ad-GFP were found to express GFP at a level higher than that of the control of liposome (11.40%). Infected adipose tissue-derived stromal cells were noted to form mineralized nodes by the use of Alizarin Red stain.
  
  
  CONCLUSIONS
  The human adipose tissue-derived stromal cells infected with Ad-GFP can express higher level of GFP, and can maintain the ability of proliferation and differentiation as the non-infected human adipose tissue-derived stromal cells do. The infected adipose tissue-derived stromal cells with Ad-GFP can track the change of adipose tissue-derived stromal cells in the study of multipotential differentiation and can serve as cellular vehicles for systemic gene delivery.
authors.affiliation: Department of Oral and Mauillofacial Surgery, West China Stomatology Hospital, Sichussan University, Chengdu 610041, China.
authors.forename: Yun-teng
authors.initials: YT
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Jing
authors.forename: Xi-zhe
authors.initials: XZ
authors.lastname: Chen
authors.forename: Ju
authors.initials: J
authors.lastname: Qiac
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Li
authors.forename: Zheng-bin
authors.initials: ZB
authors.lastname: Yan
authors.forename: Ling
authors.initials: L
authors.lastname: Wu
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Tian
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D014162:Transfection
chemical_list: D049452:Green Fluorescent Proteins