Detection of S100 protein from prostatic cancer patients using anti-S100 protein antibody immobilized on POS-PVA discs.
The S100 proteins have been extensively used as cancer biomarkers. The objectives of the present work were to immobilize the antibody anti-protein S100 to a net of semi-interpenetrated of polysiloxane and polyvinyl alcohol (POS-PVA discs), to investigate its capacity to capture S100 protein from serum and to quantify it by ELISA in sera from patients with prostatic adenocarcinoma (n = 15) and healthy individuals (n = 10). Also these values were compared to the S100 protein expression in the prostatic tissue through immunohistochemistry. The POS-PVA discs fixed about 92.8% of the offered antibody (7.75 microg of antibody per disc). The best values of the immobilized no-marked antibody anti-S100 and serum dilution were found to be 10 microg and 1:400, respectively. Optical density (OD) values for the sera of patients (0.425 +/- 0.042) with prostatic adenocarcinoma were significantly lower (P < 0.05) compared to those established for the healthy individuals (1.034 +/- 0.124). In the immunohistochemistry study no significant variations were observed in the number of positive S100 cells between prostatic adenocarcinoma (153.45 +/- 16.82) and normal prostate (147.04 +/- 18.98). These results showed a clear difference between S100 proteins expressed in tissue and presented in serum during the prostatic tissue neoplasic transformation. Sera analysis was more sensitive than immunohistochemistry S100 protein detection in the prostate tissue besides the advantage to be less invasive method.
Melo-Júnior
Mario R
MR
Laboratório de Imunopatologia Keizo Asami, LIKA, Universidade Federal de Pernambuco (UFPE), Pernambuco, Brazil. mariormj@gmail.com
Filho
Jorge Luiz S Araújo
JL
Cavalcanti
Carmelita L B
CL
Patu
Vasco José Ramos M
VJ
Beltrão
Eduardo I C
EI
Carvalho
Luiz B
LB
D014408:Biomarkers, Tumor / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000097:blood* / Q000276:immunology; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D015203:Reproducibility of Results; D009418:S100 Proteins / Q000097:blood* / Q000276:immunology; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D009418:S100 Proteins