Simulation of an oscillatory reaction of alcohol dehydrogenase in an oil/water system.
With numerical analysis of the enzyme-catalyzed reaction in the so called 'ordered bi-bi' mechanism and the model presented by Theorell and Chance (Acta Chem. Scand. 5 (1951) 1127) for alcohol dehydrogenase, oscillations of the concentrations of coenzyme and intermediate complex were obtained on gradual entry of substrate. Neither autocatalysis nor allostery was included in the reaction mechanism. The features of the reaction were that the oscillations did not occur in a limit cycle and the interval became longer with time. In addition, it was found that the oscillations with an upwardly directed peak occurred at very low concentrations of enzyme in the model of Theorell and Chance. The results were consistent with the experimentally determined data reported previously.
Hideshima
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College of Arts and Sciences, Chiba University, Chiba 260, Japan.