Activation of demineralized bone matrix by genetically engineered human bone morphogenetic protein-2 with a collagen binding domain derived from von Willebrand factor propolypeptide.
There is a large demand for new bone regeneration to restore the function during bone injuries. Bone filling materials are important in bone tissue restoration. In this study, the demineralized bone matrix (DBM) was activated with the engineering human bone morphogenetic protein-2 (BMP-2). To enhance the binding of BMP-2 to the DBM scaffolds, a collagen-binding peptide was fused to the N-terminal of BMP-2. The in vitro results showed that the engineered collagen-targeted BMP-2 (rhBMP2-v) bound to DBM scaffolds specifically and the rhBMP2-v had increased alkaline phosphatase activity in C2C12 cells. In vivo, the DBM scaffolds impregnated with rhBMP2-v showed greater effect on ectopic bone formation. Our data suggested that the collagen-based BMP-2 targeting bone repair system had greater bone inducing ability than DBM loaded with regular BMP-2.
Chen
Bing
B
Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
Lin
Hang
H
Zhao
Yannan
Y
Wang
Bin
B
Zhao
Yanhong
Y
Liu
Yunpeng
Y
Liu
Zhonghao
Z
Dai
Jianwu
J
D000818:Animals; D001665:Binding Sites; D001857:Bone Matrix / Q000378:metabolism*; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000494:pharmacology*; D002113:Calcification, Physiologic / Q000187:drug effects*; D002460:Cell Line; D003094:Collagen; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects*; D020815:Polyproteins; D015202:Protein Engineering / Q000379:methods*; D011498:Protein Precursors; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000494:pharmacology*; D014841:von Willebrand Factor / Q000235:genetics*
C525718:BMP2 protein, human; C525719:Bmp2 protein, mouse; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D020815:Polyproteins; D011498:Protein Precursors; D016212:Transforming Growth Factor beta; D014841:von Willebrand Factor; D003094:Collagen