[Spectrometry study on the interaction of Magdala red and bovine serum albumin].
In the present paper, the interaction of Magdala red and bovine serum albumin(BSA) was studied by UV-absorption and fluorescence spectrometry. The results of UV-absorption spectrometry show that the way of the interaction of Magdala red and BSA should obey the Scatchard Model. In other words, Magdala should be combined to some certain regions of BSA. Meanwhile, taking advantage of fluorescence quenching, the reaction mechanism of Magdala with BSA was explored. Protein molecule conformation change is discussed by synchronous fluorescence spectrometry.
Shi
Yan
Y
Key Laboratory of Food Science of MOE, Nanchang University, Nanchang 330047, China.
Ye
Fan-Di
FD
Zheng
Wei-Wan
WW
D000818:Animals; D002417:Cattle; D009281:Naphthalenes / Q000737:chemistry*; D011719:Pyrazines / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D009281:Naphthalenes; D011719:Pyrazines; C412337:magdala red; D012710:Serum Albumin, Bovine