title: Elevated serum 8-oxo-dG in hemodialysis patients: a marker of systemic inflammation?
abstract: Does inflammation, as assessed by high sensitivity C-reactive protein (hs-CRP), in patients with end-stage renal disease (ESRD) tightly associate with increased serum levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8- oxo-dG)? Increased oxidative stress and inflammation have both been highlighted among several nontraditional risk factors for cardiovascular disease, which is the main cause of mortality in ESRD patients. In contrast to oxidative stress effects on proteins and lipids, DNA base damage has not been well demonstrated in ESRD. Two groups of hemodialysis patients were studied, one group with persistent inflammation (n = 13, with constant elevation of CRP > 10 mg/L for 6 months) and one group of noninflamed patients (n = 19, with constant CRP < 10 mg/L for 6 months). Serum 8-oxo-dG was significantly elevated in persistent inflammation in comparison to noninflamed patients. At an individual level, a significant correlation was found between serum 8-oxo-dG and hsCRP. Extracellular 8-oxo-dG leads to intracellular oxidative damage on the nucleotide pool, thus providing a sensitive marker for inflammatory response. Serum levels of 8-oxo-dG, in combination with other inflammatory markers, serve as useful diagnostic tools for identification of patients in risk for inflammatory complications.
authors.affiliation: Department of Genetics, Microbiology and Toxicology, Stockholm University, Stockholm, Sweden.
authors.forename: Siamak
authors.initials: S
authors.lastname: Haghdoost
authors.forename: Yukio
authors.initials: Y
authors.lastname: Maruyama
authors.forename: Roberto
authors.initials: R
authors.lastname: Pecoits-Filho
authors.forename: Olof
authors.initials: O
authors.lastname: Heimburger
authors.forename: Astrid
authors.initials: A
authors.lastname: Seeberger
authors.forename: Björn
authors.initials: B
authors.lastname: Anderstam
authors.forename: Mohamed E
authors.initials: ME
authors.lastname: Suliman
authors.forename: Stefan
authors.initials: S
authors.lastname: Czene
authors.forename: Bengt
authors.initials: B
authors.lastname: Lindholm
authors.forename: Peter
authors.initials: P
authors.lastname: Stenvinkel
authors.forename: Mats
authors.initials: M
authors.lastname: Harms-Ringdahl
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000032:analysis; D004247:DNA / Q000276:immunology; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000150:complications / Q000175:diagnosis*; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000150:complications / Q000276:immunology; D009711:Nucleotides / Q000276:immunology; D018384:Oxidative Stress / Q000276:immunology; D011237:Predictive Value of Tests; D006435:Renal Dialysis
chemical_list: D015415:Biomarkers; D009711:Nucleotides; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002097:C-Reactive Protein; D004247:DNA; D003849:Deoxyguanosine