title: Analysis of triazines by capillary electrochromatography/electrospray ionization-mass spectrometry using a low-flow sheath liquid interface.
abstract: CEC-MS has been used for the analysis of eight-triazine herbicides. It showed significantly better S/N ratio than reversed EOF CE-MS and MEKC-MS, due to the lack of a surfactant in the separation buffer. By optimizing the pH, the organic content of the running buffer, and the separation potential, optimal separation was achieved within 18 min using a running buffer of pH 7.0, containing 70% v/v ACN, and an applied voltage of 17 kV. Gradient CEC showed superior separation when compared with isocratic elution. The combination of a tapered CEC column and a low-flow interface confers several advantages including better sensitivity, low dead volume, and independent control of the conditions used for CEC separation and ESI analysis.
authors.affiliation: Department of Chemistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Chia-Hao
authors.initials: CH
authors.lastname: Chang
authors.forename: Chao-Jung
authors.initials: CJ
authors.lastname: Chen
authors.forename: Yu-Chieh
authors.initials: YC
authors.lastname: Chuang
authors.forename: Guor-Rong
authors.initials: GR
authors.lastname: Her
mesh_terms: D053801:Capillary Electrochromatography / Q000379:methods*; D006540:Herbicides / Q000032:analysis / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014227:Triazines / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D006540:Herbicides; D014227:Triazines