title: Fine mapping of a pistilloid-stamen (PS) gene on the short arm of chromosome 1 in rice.
abstract: A novel floral organ mutant of rice (Oryza sativa L. subsp. indica), termed pistilloid-stamen (ps) here, has flowers with degenerated lemma and palea, with some stamens transformed into pistils and pistil-stamen chimeras. Genetic analysis confirmed that the ps trait is controlled by a single recessive gene. F2 and F3 segregation populations derived from PS ps heterozygote crossed with Oryza sativa subsp. indica 'Luhui-17' (PS PS) were used for molecular mapping of the gene using simple sequence repeat (SSR) markers. With 97 recessive individuals from an F2 segregation population, the ps locus was preliminarily mapped 6.2 cM distal to marker RM6324 and 3.1 cM proximal to marker RM6340 in the terminal region of the short arm of chromosome 1. With a large F3 segregation population, the gene was fine-mapped between markers RM6470 and RM1141, at distances of 0.10 and 0.03 cM to each marker, respectively. The position of the ps gene was finally located within a 20 kb physical region containing 3 annotated putative genes. One of them, encoding a protein with a single C2H2 zinc-finger domain, may be the candidate gene for PS.
authors.affiliation: Rice Research Institute, Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Southwest University, Chongqing, PR China.
authors.forename: Hongfa
authors.initials: H
authors.lastname: Luo
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Li
authors.forename: Zhenglin
authors.initials: Z
authors.lastname: Yang
authors.forename: Bingqiang
authors.initials: B
authors.lastname: Zhong
authors.forename: Rong
authors.initials: R
authors.lastname: Xie
authors.forename: Maozhi
authors.initials: M
authors.lastname: Ren
authors.forename: Da
authors.initials: D
authors.lastname: Luo
authors.forename: Guanghua
authors.initials: G
authors.lastname: He
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D035264:Flowers / Q000254:growth & development; D017343:Genes, Plant; D006824:Hybridization, Genetic; D018895:Microsatellite Repeats; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype
chemical_list: D017931:DNA Primers; D018744:DNA, Plant