[Investigation on bovine leukocyte adhesion deficiency].
Bovine leukocyte adhesion deficiency (BLAD) is autosomal recessive disease. The pathogeny of BLAD is genic mutation of CD18-integrins on the leukocyte. In order to know the carrier and occurrence of bovine leukocyte adhesion deficiency (BLAD) among cows age from one to six years old in China, 1,000 cows were investigated by means of amplifying a CD18 gene fragment via reverse transcriptase-PCR followed by restriction digestion with Taq I. Results showed that 19 cows were BLAD carriers, indicating that the BLAD carrier rate was 1.9 percent. In addition, one cow was found to have BLAD.
Ma
Jin-Zhu
JZ
College of Life Science and Biotechnology, Heilongjiang August First Land Reclamation University, Daqing 163319, China. majinzhu1976@163.com
Cui
Yu-Dong
YD
Zhu
Zhan-Bo
ZB
Cao
Hong-Wei
HW
Piao
Fan-Ze
FZ
D000818:Animals; D018821:CD18 Antigens / Q000235:genetics / Q000378:metabolism; D002417:Cattle / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000235:genetics* / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018821:CD18 Antigens; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases