title: Expression of the Nd1 gene is down-regulated by doxorubicin at post-transcriptional level.
abstract: Doxorubicin is an anti-neoplastic agent with cardiotoxicity as a side effect. We previously demonstrated that doxorubicin treatment of mice resulted in a selective decrease in expression of the Nd1 gene, which encoded a new kelch family actin binding protein in the heart. Here we show that doxorubicin treatment also reduced the Nd1 expression in various organs of mice and cultured cell lines. The treatment of Nd1-transgenic mice and Nd1-transfectants also selectively reduced levels of the exogenous Nd1 mRNAs, whose expression was under the control of various promoters. Furthermore, the doxorubicin-induced reduction of Nd1 mRNA expression in NIH3T3 cells was inhibited by treatment of these cells with cycloheximide. Thus, the doxorubicin treatment may specifically reduce the stability of Nd1 mRNA.
authors.affiliation: Departments of Developmental Genetics (H2), Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Takamori
authors.forename: Yuji
authors.initials: Y
authors.lastname: Matsudo
authors.forename: Lisa
authors.initials: L
authors.lastname: Fujimura
authors.forename: Myengmo
authors.initials: M
authors.lastname: Kang
authors.forename: Yoshitada
authors.initials: Y
authors.lastname: Harada
authors.forename: Hideshige
authors.initials: H
authors.lastname: Moriya
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tokuhisa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hatano
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002478:Cells, Cultured; D015536:Down-Regulation; D004317:Doxorubicin / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008822:Mice, Transgenic; D041681:NIH 3T3 Cells; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D020871:RNA Stability / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D000903:Antibiotics, Antineoplastic; D047908:Intracellular Signaling Peptides and Proteins; C432129:Nd1 protein, mouse; D011506:Proteins; D012333:RNA, Messenger; D004317:Doxorubicin