Chitinase inhibitor allosamidin is a signal molecule for chitinase production in its producing Streptomyces II. Mechanism for regulation of chitinase production by allosamidin through a two-component regulatory system.
In Streptomyces sp. AJ9463, a producer of chitinase inhibitor allosamidin, allosamidin strongly enhances production of the chitinase mainly secreted to the culture broth in a chitin medium. To clarify the mechanism for regulation of the chitinase production by allosamidin, a disruption experiment of genes encoding proteins constructing a two-component regulatory system present at 5'-upstream region of the chitinase gene was performed. In the disruptant obtained, allosamidin could not promote the chitinase production, but N, N'-diacetylchitobiose, which also enhances production of the same chitinase more weakly than allosamidin, promoted the chitinase production similarly to the case observed in the wild-type strain. Furthermore, by the experiment in an inorganic salt solution, it was shown that allosamidin could not induce the chitinase production without addition of N, N'-diacetylchitobiose. These results show that allosamidin can activate transcription of the chitinase gene through the two-component regulatory system in the presence of N, N'-diacetylchitobiose.
Suzuki
Shigeo
S
Department of Applied Biological Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
Nakanishi
Eiyu
E
Ohira
Tsuyoshi
T
Kawachi
Ryu
R
Ohnishi
Yasuo
Y
Horinouchi
Sueharu
S
Nagasawa
Hiromichi
H
Sakuda
Shohei
S
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D020868:Gene Silencing; D015394:Molecular Structure; D015398:Signal Transduction; D013302:Streptomyces / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D014312:Trisaccharides / Q000737:chemistry / Q000494:pharmacology*
D004187:Disaccharides; D014312:Trisaccharides; C054851:allosamidin; D002686:Chitin; C010913:N,N-diacetylchitobiose; D002688:Chitinases; D000117:Acetylglucosamine