[Application of L-cysteine-capped nano-ZnS as a fluorescence probe to the determination of proteins in human serum].
Nanometer-sized L-cysteine-capped ZnS particles as a fluorescence probe were synthesized, and the effect of proteins on the fluorescence intensity has been investigated. With deltalambda = 190 nm, the maximum and constant synchronous fluorescence enhancement was produced at 268.0 nm and pH 5.12 in the presence of proteins. A highly sensitive synchronous fluorescence method for the rapid determination of proteins was developed. Under optimum conditions, the calibration curve was linear over the range 0.05-6.0 microg x mL(-1) for human serum albumin (HSA), and 0.05-8.0 microg x mL(-1) for gamma-globulin (gamma-G), respectively. The relative standard deviations of seven measurements were 1.90% for 1.0 microg x mL(-1) HSA, and 1.65% for 1.0 microg mL(-1) gamma-G.
Zhao
Dan-Hua
DH
Chemistry Department, Huaibei Coal Normal College, Huaibei 235000, China.
Li
Yong-Xin
YX
Zhu
Chang-Qing
CQ
D001798:Blood Proteins / Q000032:analysis*; D003545:Cysteine / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry; D013440:Sulfides / Q000032:analysis; D017967:Zinc Compounds / Q000032:analysis
D001798:Blood Proteins; D005456:Fluorescent Dyes; D013440:Sulfides; D017967:Zinc Compounds; D003545:Cysteine; C031238:zinc sulfide