Identification of Hsc70 as an influenza virus matrix protein (M1) binding factor involved in the virus life cycle.
Influenza virus matrix protein 1 (M1) has been shown to play a crucial role in the virus replication, assembly and budding. We identified heat shock cognate protein 70 (Hsc70) as a M1 binding protein by immunoprecipitation and MALDI-TOF MS. The C terminal domain of M1 interacts with Hsc70. We found that Hsc70 does not correlate with the transport of M1 to the nucleus, however, it does inhibit the nuclear export of M1 and NP, thus resulting in the inhibition of viral production. This is the first demonstration that Hsc70 is directly associated with M1 and therefore is required for viral production.
Watanabe
Ken
K
Laboratory of Molecular Biology of Infectious Agents, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Fuse
Takayuki
T
Asano
Issay
I
Tsukahara
Fujiko
F
Maru
Yoshiro
Y
Nagata
Kyosuke
K
Kitazato
Kaio
K
Kobayashi
Nobuyuki
N
D021581:Active Transport, Cell Nucleus; D000818:Animals; D002460:Cell Line; D004285:Dogs; D050883:HSC70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D011485:Protein Binding; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D050883:HSC70 Heat-Shock Proteins; D014763:Viral Matrix Proteins