High prevalence of the K65R mutation in human immunodeficiency virus type 1 subtype C isolates from infected patients in Botswana treated with didanosine-based regimens.
We analyzed the reverse transcriptase genotypes of human immunodeficiency virus type 1 subtype C viruses isolated from 23 patients in Botswana treated with didanosine-based regimens. The K65R mutation was selected either alone or together with the Q151M, S68G, or F116Y substitution in viruses from seven such individuals. The results of in vitro passage experiments were consistent with an apparent increased propensity of subtype C viruses to develop the K65R substitution.
Doualla-Bell
Florence
F
Botswana-Harvard School of Public Health AIDS Initiative Partnership for HIV Research and Education, Gaborone, Botswana.
Avalos
Ava
A
Brenner
Bluma
B
Gaolathe
Tendani
T
Mine
Madisa
M
Gaseitsiwe
Simani
S
Oliveira
Maureen
M
Moisi
Daniella
D
Ndwapi
Ndwapi
N
Moffat
Howard
H
Essex
Max
M
Wainberg
Mark A
MA
D019380:Anti-HIV Agents / Q000627:therapeutic use*; D001902:Botswana / Q000453:epidemiology; D016049:Didanosine / Q000627:therapeutic use*; D015658:HIV Infections / Q000188:drug therapy*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D009154:Mutation; D015995:Prevalence
D019380:Anti-HIV Agents; D016049:Didanosine