title: Flow characterization of a wavy-walled bioreactor for cartilage tissue engineering.
abstract: Cartilage tissue engineering requires the use of bioreactors in order to enhance nutrient transport and to provide sufficient mechanical stimuli to promote extracellular matrix (ECM) synthesis by chondrocytes. The amount and quality of ECM components is a large determinant of the biochemical and mechanical properties of engineered cartilage constructs. Mechanical forces created by the hydrodynamic environment within the bioreactors are known to influence ECM synthesis. The present study characterizes the hydrodynamic environment within a novel wavy-walled bioreactor (WWB) used for the development of tissue-engineered cartilage. The geometry of this bioreactor provides a unique hydrodynamic environment for mammalian cell and tissue culture, and investigation of hydrodynamic effects on tissue growth and function. The flow field within the WWB was characterized using two-dimensional particle-image velocimetry (PIV). The flow in the WWB differed significantly from that in the traditional spinner flask both qualitatively and quantitatively, and was influenced by the positioning of constructs within the bioreactor. Measurements of velocity fields were used to estimate the mean-shear stress, Reynolds stress, and turbulent kinetic energy components in the vicinity of the constructs within the WWB. The mean-shear stress experienced by the tissue-engineered constructs in the WWB calculated using PIV measurements was in the range of 0-0.6 dynes/cm2. Quantification of the shear stress experienced by cartilage constructs, in this case through PIV, is essential for the development of tissue-growth models relating hydrodynamic parameters to tissue properties.
authors.affiliation: Department of Chemical Engineering, 342 Snell Engineering Center Northeastern University, Boston, Massachusetts 02115, USA.
authors.forename: Bahar
authors.initials: B
authors.lastname: Bilgen
authors.forename: Philippe
authors.initials: P
authors.lastname: Sucosky
authors.forename: G Paul
authors.initials: GP
authors.lastname: Neitzel
authors.forename: Gilda A
authors.initials: GA
authors.lastname: Barabino
mesh_terms: D000818:Animals; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods; D002356:Cartilage / Q000378:metabolism; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D004867:Equipment Design; D005109:Extracellular Matrix; D006801:Humans; D040542:Mechanotransduction, Cellular; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*