title: Expression of germination-related enzymes, CspA, CspB, CspC, SleC, and SleM, of Clostridium perfringens S40 in the mother cell compartment of sporulating cells.
abstract: In Clostridium perfringens S40, spore germination-specific enzymes are synthesized during sporulation. Previous reports have demonstrated that two cortex-lytic enzymes, SleC and SleM, and a component of germination-specific protease, CspC, are located outside the cortex as an integral part of the dormant spore. In the present study, we examined the time and compartment of these enzymes' gene expression using reverse transcription-PCR (RT-PCR) and fluorescence microscopy on green fluorescence protein (GFP)-fused proteins. These results suggested that CspABC, SleC, and SleM are synthesized in the mother cell compartment of sporulating cells, probably at stages II approximately III of sporulation, and that the expression of cspABC genes is tricistronic.
authors.affiliation: Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Masayama
authors.forename: Keishi
authors.initials: K
authors.lastname: Hamasaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Urakami
authors.forename: Seiko
authors.initials: S
authors.lastname: Shimamoto
authors.forename: Shiro
authors.initials: S
authors.lastname: Kato
authors.forename: Shio
authors.initials: S
authors.lastname: Makino
authors.forename: Tohru
authors.initials: T
authors.lastname: Yoshimura
authors.forename: Michie
authors.initials: M
authors.lastname: Moriyama
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Moriyama
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D003016:Clostridium perfringens / Q000201:enzymology* / Q000378:metabolism* / Q000502:physiology; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D006026:Glycoside Hydrolases / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013171:Spores, Bacterial / Q000378:metabolism / Q000502:physiology; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C092058:CspA protein, Clostridium; C102917:CspC protein, Clostridium; D011993:Recombinant Fusion Proteins; C077188:cold-shock protein CspB, Bacteria; D049452:Green Fluorescent Proteins; D006026:Glycoside Hydrolases; C026255:endo-N-acetylmuramidase; D009238:N-Acetylmuramoyl-L-alanine Amidase