title: A collagen-based interface construct for the assessment of cell-dependent mechanical integration of tissue surfaces.
abstract: The interface between any newly engineered tissue and pre-existing tissue is of great importance to tissue engineering; however, this process has so far been largely ignored, with few reports regarding the mechanical strength of newly integrated connective tissues surfaces. A new model system has been developed to generate a well-defined interface between two collagen lattices: one pre-contracted by resident fibroblasts and the other a cell-free wrapping gel. This construct can be cultured for prolonged periods (>2 weeks) and can also be fitted onto a mechanical testing system to measure the interface adhesive strength at the end of the culture time. Interface adhesive strength shows a six-fold increase after 1 week in culture, compared with the time-zero baseline. Observations of cell migration across the interface suggest that cell translocation in the three-dimensional matrix might play an important role in the integration process. In this new controlled geometry, normal and shear stresses at the interface can be analysed by finite element modelling and the areas at which debonding starts can be defined. The current experimental design permits solid multiple (homogeneous or heterogeneous) interface formation in vitro with a well-defined geometry and the possibility of measuring mechanical linkage. This design should enable many other factors affecting cell-driven interface strengthening to be investigated.
authors.affiliation: RFUCMS, Tissue Repair and Engineering Centre, Institute of Orthopaedics, Stanmore Campus, University College London, Brockley Hill, Stanmore, UK.
authors.forename: M
authors.initials: M
authors.lastname: Marenzana
authors.forename: D J
authors.initials: DJ
authors.lastname: Kelly
authors.forename: P J
authors.initials: PJ
authors.lastname: Prendergast
authors.forename: R A
authors.initials: RA
authors.lastname: Brown
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002465:Cell Movement; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D003238:Connective Tissue / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D020342:Finite Element Analysis; D005782:Gels / Q000737:chemistry; D008422:Materials Testing; D051381:Rats; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*
chemical_list: D005782:Gels; D003094:Collagen