Asymmetric reduction of o-chloroacetophenone with Candida pseudotropicalis 104.
The asymmetric reduction of o-chloroacetophenone 1 with Candida pseudotropicalis 104 produced the corresponding (S)-1-(2-chloro-phenyl)-ethanol 2 with the enantiomeric excess (ee >99%) without addition of any cosolvent. The cell could tolerate high ketone 1 concentration of 233.8 mmol/L (i.e., 36 g/L) with considerable reduction activity in this method. The product 2 concentration achieved 38.9 and 58.4 mmol/L with cells of 40 and 60 g(DCW) (dry cell weight)/L, respectively, in 24 h. The optimum reaction time, the effect of substrate concentration, cosubstrate type and concentration, and cell concentration in the reaction were investigated in this paper.
Xie
Qing
Q
Institute of Bioengineering, College of Material Science Chemical Engineering, Zhejiang University, Hangzhou 310027, China.
Wu
Jianping
J
Xu
Gang
G
Yang
Lirong
L
D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D001711:Biotransformation; D002175:Candida / Q000378:metabolism*; D002384:Catalysis; D002625:Chemistry, Organic / Q000379:methods; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D008956:Models, Chemical; D009249:NADP; D013237:Stereoisomerism; D002721:omega-Chloroacetophenone / Q000737:chemistry*
D009249:NADP; D002721:omega-Chloroacetophenone; D005947:Glucose