Ab initio resolution measurement for single particle structures.
A computational method is described that allows the measurement of the signal-to-noise ratio and resolution of a three-dimensional structure obtained by single particle electron microscopy and reconstruction. The method does not rely on the availability of the original image data or the calculation of several structures from different parts of the data that are needed for the commonly used Fourier Shell Correlation criterion. Instead, the correlation between neighboring Fourier pixels is calculated and used to distinguish signal from noise. The new method has been conveniently implemented in a computer program called RMEASURE and is available to the microscopy community.
Sousa
Duncan
D
Howard Hughes Medical Institute and Department of Biochemistry, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454, USA.
Grigorieff
Nikolaus
N
D004926:Escherichia coli; D005583:Fourier Analysis; D007089:Image Enhancement / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D046911:Macromolecular Substances / Q000737:chemistry; D008854:Microscopy, Electron / Q000379:methods*; D008958:Models, Molecular; D012270:Ribosomes / Q000737:chemistry; D019837:Ryanodine Receptor Calcium Release Channel / Q000737:chemistry
D046911:Macromolecular Substances; D019837:Ryanodine Receptor Calcium Release Channel