CTX-M-1- and CTX-M-15-type beta-lactamases in clinical Escherichia coli isolates recovered from food-producing animals in France.
Clinical Escherichia coli strains with resistance or variable susceptibility to third-generation cephalosporins were detected in cattle, swine and poultry in France. These strains were shown to produce extended-spectrum beta-lactamases (ESBLs), with CTX-M-1- and CTX-M-15-type beta-lactamases being responsible for this phenotype. The bla(CTX-M-1) gene was encountered most commonly and was characterised in seven E. coli strains isolated from cattle, swine and poultry, whereas bla(CTX-M-15) was identified in one E. coli isolated from cattle. These genes were located on a conjugative plasmid and were linked to the insertion sequence ISEcp1, which could have contributed to dissemination of the resistance gene. No epidemiological link between the strains was determined by pulsed-field gel electrophoresis, although two plasmids were identical in two strains isolated from swine and in two strains isolated from cattle and poultry. Thus, this study describes the emergence of ESBLs in animals in France, with a probable similar prevalence rate to that observed in humans. This is a major concern because of the possibility of transfer of these genes between animal species as well as to humans, leading to treatment failures in veterinary and human medicine.
Meunier
Danièle
D
Agence Française de Sécurité Sanitaire des Aliments, Unité de Bactériologie Bovine et Sécurité des Viandes, 31 avenue Tony Garnier, 69364 Lyon, France. d.meunier@lyon.afssa.fr
Jouy
Eric
E
Lazizzera
Corinne
C
Kobisch
Marylène
M
Madec
Jean-Yves
JY
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle / Q000382:microbiology*; D002439:Cefotaxime / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D018550:Cephalosporin Resistance / Q000235:genetics; D002511:Cephalosporins / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D005602:France; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011200:Poultry / Q000382:microbiology*; D013552:Swine / Q000382:microbiology*; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D004269:DNA, Bacterial; C053503:ceftiofur; D002442:Ceftazidime; D015246:Deoxyribonuclease EcoRI; C064053:CCTAGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C473109:beta-lactamase CTX-M-15; C055184:beta-lactamase TEM-3; D001618:beta-Lactamases; D002439:Cefotaxime