title: Bradykinin release and inactivation in brain of rats submitted to an experimental model of Alzheimer's disease.
abstract: The kallikrein-kinin system is involved in a variety of physiological and pathological processes. Components of this system, identified in rat and human brains, can be altered in neurodegenerative processes such as Alzheimer's disease. Here, we studied kinin release and its inactivation in rats submitted to chronic cerebroventricular infusion of beta-amyloid (Abeta) peptide. Neurodegeneration was confirmed by histological analysis of brain samples. In cerebrospinal fluid of animals infused with Abeta, bradykinin concentration was increased, as determined by radioimmunoassay. However, in the brain of Abeta group, we only detected the tripeptide Arg-Pro-Pro, purified by reversed-phase chromatography and characterized by liquid chromatography-electrospray ionization mass spectrometry. This fragment of bradykinin indicated the possible participation of kinin-processing enzymes in the brain such as a prolyl oligopeptidase.
authors.affiliation: Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Rua Três de Maio 100, 04044-020, S. Paulo, SP, Brazil. iores.bioq@epm.br
authors.forename: Lígia M
authors.initials: LM
authors.lastname: Iores-Marçal
authors.forename: Tânia A
authors.initials: TA
authors.lastname: Viel
authors.forename: Hudson Sousa
authors.initials: HS
authors.lastname: Buck
authors.forename: Viviane A
authors.initials: VA
authors.lastname: Nunes
authors.forename: Andrezza J
authors.initials: AJ
authors.lastname: Gozzo
authors.forename: Ilana
authors.initials: I
authors.lastname: Cruz-Silva
authors.forename: Antonio
authors.initials: A
authors.lastname: Miranda
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Shimamoto
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Ura
authors.forename: Mariana S
authors.initials: MS
authors.lastname: Araujo
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D000818:Animals; D001920:Bradykinin / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D004195:Disease Models, Animal; D051381:Rats
chemical_list: D001920:Bradykinin