title: Identification of glycoproteins in human cerebrospinal fluid with a complementary proteomic approach.
abstract: Biomarkers are pressingly needed to assist with the clinical diagnosis of neurodegenerative diseases and/or the monitoring of disease progression. Glycoproteins are enriched in bodily fluids such as human cerebrospinal fluid (CSF), an ideal source for discovering biomarkers due to its proximity to the central nervous system (CNS), and consequently can serve as diagnostic and/or therapeutic markers for CNS diseases. We report here an in-depth identification of glycoproteins in human CSF using a complementary proteomic approach which integrated hydrazide chemistry and lectin affinity column for glycoprotein enrichment, followed by multidimensional chromatography separation and tandem mass spectrometric analysis. Using stringent criteria, a total of 216 glycoproteins, including many low-abundance proteins, was identified with high confidence. Approximately one-third of these proteins was already known to be relevant to the CNS structurally or functionally. This investigation, for the first time, not only categorized many glycoproteins in human CSF but also expanded the existing overall CSF protein database.
authors.affiliation: Department of Pathology, University of Washington, Seattle, Washington 98104, USA.
authors.forename: Sheng
authors.initials: S
authors.lastname: Pan
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Quinn
authors.forename: Elaine R
authors.initials: ER
authors.lastname: Peskind
authors.forename: Dana
authors.initials: D
authors.lastname: Waichunas
authors.forename: Jake T
authors.initials: JT
authors.lastname: Wimberger
authors.forename: Jinghua
authors.initials: J
authors.lastname: Jin
authors.forename: Jane G
authors.initials: JG
authors.lastname: Li
authors.forename: David
authors.initials: D
authors.lastname: Zhu
authors.forename: Catherine
authors.initials: C
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000134:cerebrospinal fluid; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006023:Glycoproteins / Q000134:cerebrospinal fluid*; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D037102:Lectins / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D006023:Glycoproteins; D006834:Hydrazines; D037102:Lectins