Engineered matrix coatings to modulate the adhesion of CD133+ human hematopoietic progenitor cells.
Interactions of hematopoietic progenitor cells (HPC) with their local microenvironments in the bone marrow are thought to control homing, differentiation, and self-renewal of the cells. To dissect the role of extracellular matrix (ECM) components of the niche microenvironment, a set of well-defined ECM coatings including fibronectin, heparin, heparan sulphate, hyaluronic acid, tropocollagen I, and co-fibrils of collagen I with heparin or hyaluronic acid was prepared and analysed with respect to the attachment of human CD133+ HPC in vitro. The extension of the adhesion areas of individual cells as well as the fraction of adherent cells were assessed by reflection interference contrast microscopy (RICM). Intense cell-matrix interactions were found on surfaces coated with fibronectin, heparin, heparan sulphate, and on the collagen I based co-fibrils. Insignificant adhesion was found for tropocollagen I and hyaluronic acid. The strongest adhesion of HPC was observed on fibronectin with contact areas of about 7 microm(2). Interaction of HPC with coatings consisting of heparin, heparan sulphate, and co-fibrils result in small circular shaped contact zones of 3 microm(2) pointing to another, less efficient, adhesion mechanism. Analysing the specificity of cell-matrix interaction by antibody blocking experiments suggests an integrin(alpha(5)beta(1))-specific adhesion on fibronectin, while adhesion on heparin was shown to be mediated by selectins (CD62L). Taken together, our data provide a basis for the design of advanced culture carriers supporting site-specific proliferation or differentiation of HPC.
Franke
Katja
K
Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Strasse 6, 01069 Dresden, Germany.
Pompe
Tilo
T
Bornhäuser
Martin
M
Werner
Carsten
C
D000071916:AC133 Antigen; D015703:Antigens, CD / Q000096:biosynthesis*; D001854:Bone Marrow Cells / Q000166:cytology; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry; D006023:Glycoproteins / Q000096:biosynthesis*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006493:Heparin / Q000737:chemistry; D006497:Heparan Sulfate / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D010455:Peptides; D011108:Polymers / Q000737:chemistry*
D000071916:AC133 Antigen; D015703:Antigens, CD; D020099:Coated Materials, Biocompatible; D003094:Collagen; D005353:Fibronectins; D006023:Glycoproteins; D006493:Heparin; D006497:Heparan Sulfate; D010455:Peptides; D011108:Polymers; C000606023:PROM1 protein, human