title: Metabolic design of macroscopic bioreaction models: application to Chinese hamster ovary cells.
abstract: The aim of this paper is to present a systematic methodology to design macroscopic bioreaction models for cell cultures based upon metabolic networks. The cell culture is seen as a succession of phases. During each phase, a metabolic network represents the set of reactions occurring in the cell. Then, through the use of the elementary flux modes, these metabolic networks are used to derive macroscopic bioreactions linking the extracellular substrates and products. On this basis, as many separate models are obtained as there are phases. Then, a complete model is obtained by smoothly switching from model to model. This is illustrated with batch cultures of Chinese hamster ovary cells.
authors.affiliation: Centre for Systems Engineering and Applied Mechanics, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium. provost@inma.ucl.ac.be
authors.forename: A
authors.initials: A
authors.lastname: Provost
authors.forename: G
authors.initials: G
authors.lastname: Bastin
authors.forename: S N
authors.initials: SN
authors.lastname: Agathos
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Schneider
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000378:metabolism*; D003198:Computer Simulation; D006224:Cricetinae; D003412:Cricetulus; D004734:Energy Metabolism / Q000502:physiology*; D008954:Models, Biological; D009097:Multienzyme Complexes / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D012984:Software; D012985:Software Design
chemical_list: D009097:Multienzyme Complexes