title: Mapping the functional domains of yeast NMD3, the nuclear export adapter for the 60 S ribosomal subunit.
abstract: Nuclear export of the large ribosomal subunit requires the adapter protein Nmd3p to provide a leucine-rich nuclear export signal that is recognized by the export receptor Crm1. Nmd3p binds to the pre-60 S subunit in the nucleus. After export to the cytoplasm, the release of Nmd3p depends on the ribosomal protein Rpl10p and the GTPase Lsg1p. Here, we have carried out a mutational analysis of Nmd3 to better define the domains responsible for nucleocytoplasmic shuttling and ribosome binding. We show that mutations in two regions of Nmd3p affect 60 S binding, suggesting that its binding to the subunit is multivalent.
authors.affiliation: Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78172-1095, USA.
authors.forename: John
authors.initials: J
authors.lastname: Hedges
authors.forename: Yen-I
authors.initials: YI
authors.lastname: Chen
authors.forename: Matthew
authors.initials: M
authors.lastname: West
authors.forename: Cyril
authors.initials: C
authors.lastname: Bussiere
authors.forename: Arlen W
authors.initials: AW
authors.lastname: Johnson
mesh_terms: D021581:Active Transport, Cell Nucleus; D000483:Alleles; D000595:Amino Acid Sequence; D004252:DNA Mutational Analysis; D017353:Gene Deletion; D008954:Models, Biological; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D017354:Point Mutation; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics*; D012270:Ribosomes / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics*
chemical_list: C117894:NMD3 protein, S cerevisiae; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins