Sweet Potato [Ipomoea batatas (L.) Lam].
Among the available transformation methods reported on sweet potato, Agrobacterium tumefaciens-mediated transformation is more successful and desirable. Stem explants have shown to be ideal for the transformation of sweet potato because of their ready availability as explants, the simple transformation process, and high-frequency-regeneration via somatic embryogenesis. Under the two-step kanamycin-hygromycin selection method and using the appropriate explants type (stem explants), the efficiency of transformation can be considerably improved in cv. Beniazuma. The high efficiency in the transformation of stem explants suggests that the transformation protocol described in this chapter warrants testing for routine stable transformation of diverse varieties of sweet potato.
Song
Guo-qing
GQ
Plant Transformation Center, Department of Horticulture, Michigan State University, East Lansing, MI, USA.
Yamaguchi
Ken-ichi
K
D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D018920:Coculture Techniques; D003470:Culture Media; D005822:Genetic Vectors; D027723:Ipomoea batatas / Q000235:genetics* / Q000254:growth & development; D018547:Plant Stems / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000033:anatomy & histology / Q000254:growth & development; D014170:Transformation, Genetic
D003470:Culture Media