title: Carboxymethyl chitin-glucan enriched diet exhibits protective effects against oxidative DNA damage induced in freshly isolated rat cells.
abstract: The connection between dietary intake of carboxymethyl chitin-glucan (CM-CG, approximately 200 mg/kg body weight, during 21 days) and the response of freshly isolated rat cells to genotoxic treatment with a combination of photosensitizer Methylene Blue and visible light (MB+VL) was evaluated in presented study. Blood lymphocytes, testicular cells, and hepatocytes were isolated from rats fed by a standard or CM-CG enriched diet and in ex vivo conditions challenged with oxidative agent. Induced DNA damage was assessed using a modified comet assay. When added to the diet, CM-CG itself did not induce any negative effect on the health condition of animals or on level of DNA breaks in rat cells. Moreover, the cells isolated from CM-CG fed animals were more resistant to oxidative stress induced by visible light-excited Methylene Blue. In conclusion, we have demonstrated that carboxymethyl chitin-glucan represents a natural fungal polysaccharide that is able to exert antimutagenic properties upon application in diet.
authors.affiliation: Cancer Research Institute, Slovak Academy of Sciences, 83391 Bratislava, Slovak Republic. monika.lazarova@savba.sk
authors.forename: M
authors.initials: M
authors.lastname: Lazarová
authors.forename: J
authors.initials: J
authors.lastname: Lábaj
authors.forename: G
authors.initials: G
authors.lastname: Kogan
authors.forename: D
authors.initials: D
authors.lastname: Slamenová
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000008:administration & dosage*; D002478:Cells, Cultured; D002686:Chitin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004032:Diet; D005936:Glucans / Q000008:administration & dosage*; D008297:Male; D008751:Methylene Blue / Q000633:toxicity; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016587:Antimutagenic Agents; D005936:Glucans; D016877:Oxidants; C091649:carboxymethyl-chitin-glucan; D002686:Chitin; D008751:Methylene Blue