title: Inhibitory effects of Lactobacillus acidophilus lysates on the cytotoxic activity of shiga-like toxin 2 produced from Escherichia coli O157:H7.
abstract: OBJECTIVE
  The purpose of this study was to characterize the degree to which four cell lysates obtained from Lactobacillus acidophilus strains affected the cytotoxic activity of Escherichia coli O157:H7 in vitro and in vivo.
  
  
  RESULTS
  In a cytotoxic inhibition test that used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and toxin-binding ELISA assays, the activity of shiga-like toxin 2 (Stx-2) was inhibited profoundly by the cell lysates (10 mg ml(-1)) from two strains of L. acidophilus A4 and 30SC (>85% of survival rates compared with the control) among the five strains tested. In particular, a significant decline in the virulence level of E. coli O157:H7, under the presence of the cell lysates of L. acidophilus A4, was observed by killing assay of Caenorhabditis elegans in vivo model.
  
  
  CONCLUSIONS
  According to our results, L. acidophilus strains might be capable of attenuating the virulence of Stx-2 produced from E. coli O157:H7.
  
  
  CONCLUSIONS
  The cell lysates of L. acidophilus can be applied to a variety of foods, and can be used as adjuncts for the inhibition of Stx-2-mediated cytotoxicity.
authors.affiliation: Division of Food Science, Korea University, Seoul, Korea.
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
authors.forename: K-S
authors.initials: KS
authors.lastname: Han
authors.forename: J-Y
authors.initials: JY
authors.lastname: Imm
authors.forename: S
authors.initials: S
authors.lastname: Oh
authors.forename: S
authors.initials: S
authors.lastname: You
authors.forename: S
authors.initials: S
authors.lastname: Park
authors.forename: S-H
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001419:Bacteria; D017173:Caenorhabditis elegans / Q000187:drug effects; D002457:Cell Extracts / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D019453:Escherichia coli O157 / Q000472:pathogenicity*; D007779:Lactobacillus acidophilus / Q000502:physiology*; D022641:Shiga Toxin 2 / Q000037:antagonists & inhibitors* / Q000633:toxicity; D014709:Vero Cells; D000095983:Bacterial Lysates
chemical_list: D002457:Cell Extracts; D022641:Shiga Toxin 2; C030259:Broncho-Vaxom; D000095983:Bacterial Lysates