The application of PCR-ELISA to the detection of Trypanosoma congolense type savannah (TCS) in bovine blood samples.
PCR-ELISA was set up to detect strains of Trypanosoma congolense type savannah (TCS) in field samples of buffy coats. Results of PCR-ELISA and PCR were compared and the effectiveness of both techniques was also compared with the Murray's method for the detection of TCS in 257 bovine buffy coats. The PCR products were labelled with digoxigenin (DIG-dUTP) during amplification cycles of the repetitive satellite DNA. A biotinylated DNA capture probe was used to detect the PCR products by ELISA in streptavidin coated microplates. Both the PCR-ELISA and PCR were more sensitive and more specific than the Murray's method. Of the 257 buffy coats analysed by the three techniques, PCR-ELISA and PCR detected TCS in 98 and 97 buffy coats respectively, whereas the Murray's method detected only 39 samples. PCR-ELISA and PCR had almost the same sensitivity and specificity. PCR-ELISA and PCR respectively detected TCS in 39.2% and 38.6% in all the 334 samples analysed by both techniques in this study.
Sow
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Laboratoire National d'Elevage, 03 BP. 7026 Ouagadougou 03, Burkina Faso. wosamada@yahoo.fr
Sidibé
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Desquesnes
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Bengaly
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Pangui
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D000818:Animals; D002417:Cattle / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D018512:Parasitemia / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014348:Trypanosoma congolense / Q000302:isolation & purification*