Rabbit antithymocyte globulin inhibits monocyte-derived dendritic cells maturation in vitro and polarizes monocyte-derived dendritic cells towards tolerogenic dendritic cells expressing indoleamine 2,3-dioxygenase.
BACKGROUND
Rabbit antithymocyte globulin (rATG) is a polyclonal mixture of immunoglobulin (Ig) G. It is used to prevent graft rejection and also graft versus host disease after transplantation. Its effect on lymphocyte function has been widely studied. Dendritic cells are central actors of the immune system. As antigen presenting cells, they are able to initiate, stimulate, and modulate immune responses.


METHODS
In this study, we investigated rATG effects on in vitro differentiation and maturation of monocyte-derived dendritic cells (Mo-DCs).


RESULTS
rATG inhibited maturation of immature Mo-DCs and allowed the generation of dendritic cells expressing ILT-3, CD123, CCR6 but not CCR7 and producing Indoleamine 2,3-dioxygenase mRNA as well as interferon-alpha.


CONCLUSIONS
rATG polarizes in vitro Mo-DCs towards tolerogenic dendritic cells.
Gillet-Hladky
Stéphanie
S
Etablissement Français du Sang, Laboratoire d'Immunologie, Lyon, France.
de Carvalho
Camila Miranda
CM
Bernaud
Janine
J
Bendahou
Christine
C
Bloy
Christian
C
Rigal
Dominique
D
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D005434:Flow Cytometry; D006084:Graft Rejection / Q000517:prevention & control; D006086:Graft vs Host Disease / Q000517:prevention & control; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007108:Immune Tolerance; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D015847:Interleukin-4 / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D011817:Rabbits
D000961:Antilymphocyte Serum; D016207:Cytokines; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor