Optimization of culture conditions for production of antimalarial menisporopsin A by the seed fungus Menisporopsis theobromae BCC 4162.
OBJECTIVE
The aim of this work was to optimize the production of a novel antimaralial menisporopsin A by the seed fungus Menisporopsis theobromae BCC 4162.


RESULTS
Fungal cultures were grown in shake flasks at 25 degrees C in the basal medium with varying carbon and nitrogen sources, aeration rates and initial pH levels. The optimal carbon and nitrogen sources that improved the production of menisporopsin A were 1% fructose and 2.5% meat extract respectively. The production was further enhanced when the culture incubated on a shaker at 200 rev min(-1) with an initial pH of 8. The yield of menisporopsin A cultured under the optimized conditions was increased from 348.30 (obtained from basal medium) to 889.02 mg l(-1), and the cultivation time was reduced from 28 to only 4 days. As a result, the productivity of menisporopsin A was greatly enhanced to 222.26 mg l(-1) day(-1) which is 18-fold higher than that of basal conditions. Larger scale production in a fermenter was also achieved, yielding menisporopsin A at a maximal level of 594.32 mg l(-1) in 4 days.


CONCLUSIONS
The optimized culture conditions for menisporopsin A production by M. theobromae BCC 4162 was the cultivation under shaking or agitation at 25 degrees C in fructose-meat extract medium with an initial pH of 8.


CONCLUSIONS
The production of menisporopsin A in a fermenter with a relatively short incubation period could be valuable for further utilization for chemical structure modification and derivatization.
Madla
S
S
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Klong Luang, Pathumthani, Thailand.
Kittakoop
P
P
Wongsa
P
P
D000962:Antimalarials / Q000302:isolation & purification*; D002244:Carbon / Q000378:metabolism; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D018942:Macrolides / Q000302:isolation & purification* / Q000378:metabolism; D003904:Mitosporic Fungi / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism
D000962:Antimalarials; D018942:Macrolides; C485925:menisporopsin A; D002244:Carbon; D009584:Nitrogen