Preparation of plant samples for 2-d electrophoresis.
Plant protein samples are very difficult to extract and prepare for 2-D electrophoresis, because polyphenols can build irreversible complexes with the proteins, the protein concentrations are relatively low, polysaccharides and lipids can cause severe disturbances in the 2-D gel.
Westermeier
Reiner
R
GE Healthcare Life Sciences, Oskar-Schlemmer-Strasse 11, D-80807 Munich, Germany. reiner.westermeier@ge.com
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008565:Membrane Proteins / Q000302:isolation & purification; D010936:Plant Extracts / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000302:isolation & purification*; D012995:Solubility; D013048:Specimen Handling / Q000379:methods*; D003313:Zea mays / Q000737:chemistry
D008565:Membrane Proteins; D010936:Plant Extracts; D010940:Plant Proteins