title: Effect of thermal processing on the flavonols rutin and quercetin.
abstract: The current research involves the study of the thermal treatment of quercetin and rutin in an aqueous model system (cooking). These substances were heated and their degradation was followed by high-performance liquid chromatography/diode-array detection (HPLC/DAD). The influence of pH and the involvement of oxygen in the degradation were studied. HPLC/electrospray ionization multi-stage mass spectrometry (ESI-MS(n)) was used for the structural characterization of the compounds produced. The influence of the degradation of the phenolic compounds on their antioxidant properties was elucidated by a electron spin resonance (ESR) spectrometry study of the reaction samples mixed with the stabilized radical, Fremy's salt. Strong degradation of the model substances took place under weak basic and oxidative conditions. Quercetin showed the most intense degradation. Protocatechuic acid could be identified as a cleavage reaction product by analyzing its retention time and molar mass during the degradation of quercetin. The structure of a second cleavage product could be identified on the basis of ESI-MS(n) fragmentation data. Also, several structures for reaction products of oxidized quercetin are suggested. The ESR analysis showed a decrease in the antioxidant activity of the reaction samples after heat treatment in aqueous solution.
authors.affiliation: Technical University of Berlin, Institute of Food Technology and Food Chemistry, Department of Food Analysis, TIB 4/3-1, Gustav-Meyer-Allee 25, D-13355 Berlin, Germany.
authors.forename: Nadja
authors.initials: N
authors.lastname: Buchner
authors.forename: Angelika
authors.initials: A
authors.lastname: Krumbein
authors.forename: Sascha
authors.initials: S
authors.lastname: Rohn
authors.forename: Lothar W
authors.initials: LW
authors.lastname: Kroh
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044948:Flavonols / Q000737:chemistry; D005511:Food Handling / Q000379:methods*; D006358:Hot Temperature; D011794:Quercetin / Q000737:chemistry*; D012431:Rutin / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D044948:Flavonols; D012431:Rutin; D011794:Quercetin