Yeast (Saccharomyces cerevisiae).
The yeast Saccharomyces cerevisiae is one of the best characterized eukaryotic organisms. This species has enabled a detailed study of the (genetic) requirements for Agrobacterium-mediated DNA transformation. For instance research with this yeast has led to the recognition that the transforming DNA molecules integrate into the eukaryotic chromosomes either by homologous recombination, which is the preferred pathway in S. cerevisiae, or by nonhomologous end-joining. Based on the protocol for Agrobacterium-mediated transformation of S. cerevisiae methodology has been developed for the transformation of many other yeast and fungal species.
Hooykaas
Paul J J
PJ
Institute of Biology, Department of Molecular and Developmental Genetics, Leiden University, Clusius Laboratory, Leiden, The Netherlands.
den Dulk-Ras
Amke
A
Bundock
Paul
P
Soltani
Jalal
J
van Attikum
Haico
H
van Heusden
G Paul H
GP
D016960:Agrobacterium tumefaciens / Q000166:cytology / Q000235:genetics*; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D003470:Culture Media; D004269:DNA, Bacterial; D018390:Gene Targeting; D005822:Genetic Vectors; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D014170:Transformation, Genetic
D003470:Culture Media; D004269:DNA, Bacterial; C036483:T-DNA