title: Targeted lipidomics: discovery of new fatty acyl amides.
abstract: The discovery of endogenous fatty acyl amides such as N-arachidonoyl ethanolamide (anandamide), N-oleoyl ethanolamide (OEA), and N-arachidonoyl dopamine (NADA) as important signaling molecules in the central and peripheral nervous system has led us to pursue other unidentified signaling molecules. Until recently, technical challenges, particularly those associated with lipid purification and chemical analysis, have hindered the identification of low abundance signaling lipids. Improvements in chromatography and mass spectrometry (MS) such as miniaturization of high-performance liquid chromatography components, hybridization of multistage mass spectrometers and time-of-flight technology, the development of electrospray ionization (ESI) and of information-dependent acquisition, now permit rapid identification of novel, low abundance, signaling lipids.
authors.affiliation: Gill Center for Biomolecular Science, Indiana University, Bloomington, IN, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Tan
authors.forename: Heather B
authors.initials: HB
authors.lastname: Bradshaw
authors.forename: Neta
authors.initials: N
authors.lastname: Rimmerman
authors.forename: Harini
authors.initials: H
authors.lastname: Srinivasan
authors.forename: Y William
authors.initials: YW
authors.lastname: Yu
authors.forename: Jocelyn F
authors.initials: JF
authors.lastname: Krey
authors.forename: M Francesca
authors.initials: MF
authors.lastname: Monn
authors.forename: Jay Shih-Chieh
authors.initials: JS
authors.lastname: Chen
authors.forename: Sherry Shu-Jung
authors.initials: SS
authors.lastname: Hu
authors.forename: Sarah R
authors.initials: SR
authors.lastname: Pickens
authors.forename: J Michael
authors.initials: JM
authors.lastname: Walker
mesh_terms: D000577:Amides / Q000032:analysis* / Q000737:chemistry; D001095:Arachidonic Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D063388:Endocannabinoids; D005227:Fatty Acids / Q000032:analysis*; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D053284:Polyunsaturated Alkamides; D015398:Signal Transduction / Q000502:physiology
chemical_list: D000577:Amides; D001095:Arachidonic Acids; D063388:Endocannabinoids; D005227:Fatty Acids; D008055:Lipids; D053284:Polyunsaturated Alkamides; C078814:anandamide