Evaluation by Q-RTPCR of the efficacy of ribavirin complexed with beta-cyclodextrin against measles virus in a mouse encephalitis model.
The objective of this work was to study the antiviral activity of ribavirin on measles encephalitis infection when using cyclodextrins as carriers. The use of cyclic oligosaccharides can promote the activity of many drugs and the benefit of the association of ribavirin with beta-cyclodextrin has already been demonstrated in vitro. Intracranial inoculation of the rodent adapted neurovirulent CAM/RB strain of measles virus induces encephalitis in CBA/ca mice. The antiviral activity of the complex ribavirin/beta-cyclodextrin at molar ratio 1:1 has been evaluated in vivo in the above encephalitis model. CBA/ca mice were treated with daily intraperitoneal injection of ribavirin (40 mg/kg) with or without beta-cyclodextrin. The viral load in the brain of mice was quantified by real-time Reverse transcription-Polymerase chain reaction. Treatment of mice by the complex ribavirin/beta-cyclodextrin (1:1) by intraperitoneal route decreases the viral load in the brain of 1.1 and 0.7 log(10) Eq copies x mL(-1) compared to distillated water and ribavirin treatment, respectively. At the same time, free ribavirin injection shows a negligible difference compared to treatment by distillated water.
Jeulin
H
H
GEVSM-SRSMC-UMR 7565 CNRS-UHP, laboratoire de bactériologie-virologie, faculté de médecine, Vandoeuvre-les-Nancy, France. helene.jeulin@pharma.uhp-nancy.fr
Grancher
N
N
Kedzierewicz
F
F
Le Faou
A E
AE
Venard
V
V
D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D018792:Encephalitis, Viral / Q000188:drug therapy* / Q000209:etiology*; D008457:Measles / Q000150:complications*; D008459:Measles virus; D051379:Mice; D008808:Mice, Inbred CBA; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012254:Ribavirin / Q000627:therapeutic use*; D047392:beta-Cyclodextrins / Q000627:therapeutic use*
D000998:Antiviral Agents; D047392:beta-Cyclodextrins; D012254:Ribavirin; C031215:betadex