Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Mongolian gerbil model.
Helicobacter pylori is associated with gastric disorders in humans and some experimental animals, and possesses the luxS/type 2 autoinducer (AI-2) system. The effects of a specific luxS mutation on the characteristics of H. pylori were examined. On 0.3 % agar medium, motility of H. pylori HPKY08 (luxS : : cat) was significantly lower than that of wild-type H. pylori TK1402. The luxS-complemented strain HPKY21 exhibited motility comparable to that of H. pylori TK1402. It was shown that the luxS/AI-2 system plays an important role in H. pylori motility. The luxS mutant exhibited a reduced infection rate relative to the wild-type parent strain TK1402 in a Mongolian gerbil model. At 3 months after oral inoculation, lower numbers of H. pylori were detected by semi-quantitative real-time reverse transcription PCR (qRT-PCR) in luxS(-) mutant-infected gerbils than in TK1402-infected gerbils. Gastric inflammation and increased antibody titre for H. pylori were observed in TK1402-infected gerbils only.
Osaki
Takako
T
Hanawa
Tomoko
T
Manzoku
Taki
T
Fukuda
Minoru
M
Kawakami
Hayato
H
Suzuki
Hidekazu
H
Department of Internal Medicine, Keio University School of Medicine Shinjuku-ku, Tokyo, Japan.
Yamaguchi
Hiroyuki
H
Department of Basic Laboratory Sciences, School of Allied Health Sciences, Osaka University, Yamadaoka, Suita, Osaka, Japan.
Yan
Xu
X
Department of Microbiology, Kunming Medical School, Kunming, Republic of China.
Taguchi
Haruhiko
H
Kurata
Satoshi
S
Kamiya
Shigeru
S
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics*; D013437:Carbon-Sulfur Lyases / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D005407:Flagella / Q000648:ultrastructure; D005753:Gastric Mucosa / Q000382:microbiology*; D005849:Gerbillinae; D005947:Glucose / Q000494:pharmacology; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006714:Homoserine / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007783:Lactones; D008297:Male; D008855:Microscopy, Electron, Scanning; D016254:Mutagenesis, Insertional; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013272:Stomach Diseases / Q000382:microbiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria; D005947:Glucose