Assessing the potential of bacterial DNA profiling for forensic soil comparisons.
A pilot study was undertaken to evaluate DNA profiling of the bacterial community in soil as an alternative to geological methods for forensic soil comparisons. Soil samples from three different ecosystems were compared, and the variation within and between ecologically different sites was determined by using terminal restriction fragment (TRF) analysis of 16S ribosomal DNA. Comparison of TRF profiles revealed that samples from within a specific ecosystem (e.g., a field) showed a significantly higher similarity to each other than to those from another ecosystem (e.g., a forest). In addition, some profile features were unique to specific ecosystems. These features may allow the determination of characteristic profiles that will facilitate identification of ecologically different sites, so that a given sample collected from a suspect could be identified as originating from, for example, a field, rather than a forest. The implications of these preliminary findings for forensic investigations are discussed.
Heath
Lorraine E
LE
School of Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, U.K. leheath@cox.net
Saunders
Venetia A
VA
D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000032:analysis; D017753:Ecosystem; D005554:Forensic Medicine / Q000379:methods; D010865:Pilot Projects; D012150:Polymorphism, Restriction Fragment Length; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004262:DNA Restriction Enzymes