title: Synthesis, biological activity and molecular modelling of new trisubstituted 8-azaadenines with high affinity for A1 adenosine receptors.
abstract: We describe here the synthesis and biological activity of new 8-azaadenines bearing both a phenyl group on C(2) and a 9-benzyl group substituted in the ortho position with a Cl or a F atom or a CF(3) group, to verify the synergistic effect of a combination of these substitution patterns on binding with the A(1) adenosine receptors. In position N(6) aliphatic and cycloaliphatic substituents were chosen which had been shown to bind well with the A(1) receptors. Because of the high lipophilicity of these kinds of molecules, we also introduced a hydroxyalkyl substituent in the same position. The compounds obtained generally showed a very good affinity and selectivity for A(1) receptors. Some of the compounds showed K(i) in the nanomolar range, one even in the subnanomolar range (0.6 M). Molecular docking calculations were performed in order to evaluate the interaction energies between the bovine A(1) receptor model and the selected ligands, and then to correlate these energies with biological activities of the ligands as obtained from the experiments. Molecular docking analysis suggests different binding modes towards A(1) receptors that are plausible for these ligands.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Pisa, via Bonanno 6, 56126 Pisa, Italy. igiorgi@farm.unipi.it <igiorgi@farm.unipi.it>
authors.forename: Irene
authors.initials: I
authors.lastname: Giorgi
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Bianucci
authors.forename: Giuliana
authors.initials: G
authors.lastname: Biagi
authors.forename: Oreste
authors.initials: O
authors.lastname: Livi
authors.forename: Valerio
authors.initials: V
authors.lastname: Scartoni
authors.forename: Michele
authors.initials: M
authors.lastname: Leonardi
authors.forename: Daniele
authors.initials: D
authors.lastname: Pietra
authors.forename: Alessio
authors.initials: A
authors.lastname: Coi
authors.forename: Ilaria
authors.initials: I
authors.lastname: Massarelli
authors.forename: Fatena Ahmad
authors.initials: FA
authors.lastname: Nofal
authors.forename: Francesca Lidia
authors.initials: FL
authors.lastname: Fiamingo
authors.forename: Paola
authors.initials: P
authors.lastname: Anastasi
authors.forename: Giuliano
authors.initials: G
authors.lastname: Giannini
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000494:pharmacology; D058916:Adenosine A1 Receptor Antagonists; D000818:Animals; D016466:CHO Cells; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D011869:Radioligand Assay; D043682:Receptor, Adenosine A1 / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D058916:Adenosine A1 Receptor Antagonists; D008024:Ligands; D043682:Receptor, Adenosine A1; D000225:Adenine