title: Spleen-dependent turnover of CD11b peripheral blood B lymphocytes in bovine leukemia virus-infected sheep.
abstract: Lymphocyte homeostasis is determined by a critical balance between cell proliferation and death, an equilibrium which is deregulated in bovine leukemia virus (BLV)-infected sheep. We have previously shown that an excess of proliferation occurs in lymphoid tissues and that the peripheral blood population is prone to increased cell death. To further understand the mechanisms involved, we evaluated the physiological role of the spleen in this accelerated turnover. To this end, B lymphocytes were labeled in vivo using a fluorescent marker (carboxyfluorescein diacetate succinimidyl ester), and the cell kinetic parameters (proliferation and death rates) of animals before and after splenectomy were compared. We show that the enhanced cell death observed in BLV-infected sheep is abrogated after splenectomy, revealing a key role of the spleen in B-lymphocyte dynamics.
authors.affiliation: National Fund for Scientific Research, Molecular and Cellular Biology Laboratory, 13 avenue Maréchal Juin, 5030 Gembloux, Belgium.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Florins
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gillet
authors.forename: Becca
authors.initials: B
authors.lastname: Asquith
authors.forename: Christophe
authors.initials: C
authors.lastname: Debacq
authors.forename: Geneviève
authors.initials: G
authors.lastname: Jean
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schwartz-Cornil
authors.forename: Michel
authors.initials: M
authors.lastname: Bonneau
authors.forename: Arsène
authors.initials: A
authors.lastname: Burny
authors.forename: Michal
authors.initials: M
authors.lastname: Reichert
authors.forename: Richard
authors.initials: R
authors.lastname: Kettmann
authors.forename: Luc
authors.initials: L
authors.lastname: Willems
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D039481:CD11b Antigen / Q000276:immunology*; D049109:Cell Proliferation; D017931:DNA Primers; D006800:Deltaretrovirus Infections / Q000276:immunology*; D005452:Fluoresceins; D006706:Homeostasis / Q000276:immunology*; D016130:Immunophenotyping; D007700:Kinetics; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D012756:Sheep; D013154:Spleen / Q000276:immunology*; D013156:Splenectomy; D013388:Succinimides
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D039481:CD11b Antigen; D017931:DNA Primers; D005452:Fluoresceins; D013388:Succinimides