title: N-linked neutral oligosaccharides in the stratum corneum of normal and ichthyotic skin.
abstract: N-Glycan oligosaccharides are thought to play multiple, important roles in a variety of biological events. However, N-glycan profiles in the stratum corneum of human skin have not yet been studied in detail. To clarify the N-glycan profiles in the stratum corneum of normal and ichthyotic epidermis, N-glycan profiles were studied by high-performance liquid chromatography using normal human epidermal samples and scales from hyperkeratotic skin of ichthyosis patients. Chromatograms of patient scale samples showed unique alterations in three peaks eluted at 15.8, 18.8 and 26.9 min. The N-glycan profiles were significantly altered in ichthyotic hyperkeratotic skin compared with normal non-hyperkeratotic controls. These findings indicate the reduction of N-acetylglucosaminyltransferase II and fucosyltransferase 8 activities. Alteration of N-glycan structures in hyperkeratotic skin suggests the biological role of N-glycans in keratinization.
authors.affiliation: Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, 060-8638, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ito
authors.forename: Masashi
authors.initials: M
authors.lastname: Akiyama
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Rie
authors.initials: R
authors.lastname: Uematsu
authors.forename: Kisaburo
authors.initials: K
authors.lastname: Deguchi
authors.forename: James R
authors.initials: JR
authors.lastname: McMillan
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimizu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004817:Epidermis / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D007057:Ichthyosis / Q000473:pathology*; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides