Formation and stability of G-quadruplexes self-assembled from guanine-rich strands.
Electrospray ionization mass spectrometry (ESI-MS) was utilized to investigate the formation and stability of G-quadruplexes. For the 15 6-nt oligonucleotides tested, ESI-MS indicated that formation of a parallel tetramer quadruplex requires at least four continuous guanines in the 6-nt sequence. In addition, the G-rich strands prefer to employ "self-association" in the formation of the G-quadruplex rather than hybridized integration, and the thermodynamic-stability order of these three G-quadruplexes is Q(2)>Q(1)>Q(3).
Zhou
Jiang
J
Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Yuan
Gu
G
Liu
Junjun
J
Zhan
Chang-Guo
CG
D001665:Binding Sites; D001667:Binding, Competitive; D018360:Crystallography, X-Ray; D006147:Guanine / Q000737:chemistry*; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D009757:Nylons / Q000138:chemical synthesis / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D010569:Perylene / Q000031:analogs & derivatives / Q000737:chemistry; D010881:Piperidones / Q000737:chemistry; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013816:Thermodynamics
D008024:Ligands; C456408:N,N'-bis(3-(4-morpholino)propyl)-3,4,9,10-perylenetetracarboxylic acid diimide; D009757:Nylons; D009838:Oligodeoxyribonucleotides; D010881:Piperidones; D010569:Perylene; D006147:Guanine