title: Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae.
abstract: To improve ethanol production in Saccharomyces cerevisiae, two yeast strains were constructed. In the mutant KAM-3, the FPS1 gene, which encodes a channel protein responsible for glycerol export, was deleted. The mutant KAM-11 had the GLT1 gene (encoding glutamate synthase) placed under the PGK1 promoter while having the FPS1 deletion. Growth rate and biomass concentration remained virtually unchanged with the mutant KAM-11, compared to that of the parent. Over-expression of GLT1 by the PGK1 promoter along with FPS1 deletion resulted in a 14% higher ethanol production and a 30% lower glycerol formation compared to the parental strain under anaerobic fermentation conditions. Furthermore, acetate and pyruvic acid formation was also reduced in order for cells to maintain redox balance.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
authors.forename: Qing-Xue
authors.initials: QX
authors.lastname: Kong
authors.forename: Ji-Guang
authors.initials: JG
authors.lastname: Gu
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Cao
authors.forename: Ai-Li
authors.initials: AL
authors.lastname: Zhang
authors.forename: Xun
authors.initials: X
authors.lastname: Chen
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Zhao
mesh_terms: D004526:Efficiency; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000235:genetics; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005970:Glutamate Synthase / Q000235:genetics*; D007218:Industrial Microbiology / Q000379:methods; D008565:Membrane Proteins / Q000235:genetics*; D008954:Models, Biological; D030781:Organisms, Genetically Modified; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D015854:Up-Regulation
chemical_list: C093263:FPS1 protein, S cerevisiae; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D005970:Glutamate Synthase