title: Purification and characterization of an N-acetyl-D-galactosamine-specific lectin from seeds of chickpea (Cicer arietinum L.).
abstract: A novel lectin (CAA-II) was isolated and purified from the seeds of Cicer arietinum by ammonium sulphate fractionation and affinity chromatography on an N-acetyl-D-galactosamine-linked agarose column. The lectin is composed of four identical subunits of 30 kDa and the molecular mass of the native lectin was estimated to be 120 kDa by gel filtration chromatography and confirmed by mass spectrometry. The lectin showed agglutination activity against rabbit erythrocytes (trypsin-treated and untreated) as well as against human erythrocytes. Haemagglutination inhibition assays showed that the lectin is a galactose-specific protein having a high affinity for N-acetyl-D-galactosamine. The molecular weight, haemagglutination pattern, carbohydrate specificity and N-terminal amino acid sequence indicated that the lectin is clearly distinct from the previously reported chickpea lectin CAA-I.
authors.affiliation: NRC on Plant Biotechnology, IARI, New Delhi, India.
authors.forename: Insaf A
authors.initials: IA
authors.lastname: Qureshi
authors.forename: Prasanta
authors.initials: P
authors.lastname: Dash
authors.forename: P S
authors.initials: PS
authors.lastname: Srivastava
authors.forename: K R
authors.initials: KR
authors.lastname: Koundal
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism*; D000595:Amino Acid Sequence; D029863:Cicer / Q000737:chemistry* / Q000378:metabolism; D006386:Hemagglutination Tests; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism
chemical_list: D037102:Lectins; D000116:Acetylgalactosamine