Tumor antigen occurs in N-glycan of royal jelly glycoproteins: honeybee cells synthesize T-antigen unit in N-glycan moiety.
In our previous paper (Kimura, Y., et al., Biosci. Biotechnol. Biochem., 67, 1852-1856, 2003), we found that a complex type N-glycans containing beta1-3 galactose residue occurs on royal jelly glycoproteins. During structural analysis of minor components of royal jelly N-glycans, we found complex type N-glycans bearing both galactose and N-acetylgalactosamine residues. Detailed structural analysis of pyridylaminated oligosaccharide revealed that the newly found N-glycan had a complex type structure harboring a tumor marker (T-antigen) unit: Galbeta1-3GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-6 (Galbeta1-3GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-3) Manbeta1-4GlcNAcbeta1-4GlcNAc. To our knowledge, this may be the first report of the presence of the T-antigen unit in the N-glycan moiety of eucaryotic glycoproteins.
Kimura
Yoshinobu
Y
Department of Biofunctional Chemistry, Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Japan. yosh8mar@cc.okayama-u.ac.jp
Ushijima
Takayuki
T
Maeda
Megumi
M
Hama
Yoichiro
Y
Kimura
Mariko
M
Okihara
Kiyoshi
K
Sugimoto
Hiroyuki
H
Yamada
Hideo
H
D000818:Animals; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000302:isolation & purification*; D000957:Antigens, Viral, Tumor / Q000096:biosynthesis*; D001516:Bees / Q000737:chemistry / Q000276:immunology*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000737:chemistry; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000096:biosynthesis; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D021241:Spectrometry, Mass, Electrospray Ionization; D000099219:Royal Jelly
D000951:Antigens, Neoplasm; D000957:Antigens, Viral, Tumor; D005227:Fatty Acids; D006023:Glycoproteins; D010446:Peptide Fragments; D011134:Polysaccharides; D000099219:Royal Jelly