Ultra-sensitive colorimetric method to quantitate hundreds of polynucleotide molecules by gold nanoparticles with silver enhancement.
An ultra-sensitive colorimetric method to quantitate hundreds of polynucleotide molecules by gold nanoparticles with silver enhancement has been developed. The hybridization products from the target polynucleotides with biotin-labeled probes and gold nanoparticle-functioned oligonucleotides were immobilized to microplates via avidin-biotin system, and the absorbance signals of gold nanoparticles were amplified by silver enhance solution. This sandwich colorimetric assay can detect as few as 600 molecules for single-strand oligonucleotides and as few as 6000 molecules for double-strand polynucleotides in a 50 microL reaction system.
Li
Bo-Sheng
BS
MOE Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei Province, China.
Zhao
Li-Fan
LF
Zhang
Chi
C
Hei
Xiao-Han
XH
Li
Fang
F
Li
Xiao-Bo
XB
Shen
Jie
J
Li
Yuan-Yuan
YY
Huang
Qiang
Q
Xu
Shun-Qing
SQ
D001360:Avidin / Q000737:chemistry; D001483:Base Sequence; D001710:Biotin / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008969:Molecular Sequence Data; D049329:Nanostructures; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D011119:Polynucleotides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D013499:Surface Properties
D009841:Oligonucleotides; D011119:Polynucleotides; D001360:Avidin; D012834:Silver; D001710:Biotin; D006046:Gold