[Chemotherapy guidance of the drug sensitivity test in vitro in human laryngeal squamous cell carcinoma].
OBJECTIVE
Consequently, strong emphasis has been placed on finding appropriate approach to achieve optimal treatment outcome. This study analyzed the drug sensitivity of Hep-2 cells at concentration-dependent and time-dependent in vitro.


METHODS
Nonradioactive cell counting Kit-8 (Dojindo, Kumamoto, Japan) was used to asses growth inhibition of cancer cells by 5-FU, Carboplatin and paclitaxel (TXL) against each concentration and exposure time. We selected optimal dose and administration regimen through comparing all the cure effect.


RESULTS
The drug sensitivity test data revealed that 5-FU inhibited cell growth dependently on concentration and time, that TXL did dependently on time, and that Carboplatin did dependently on concentration.


CONCLUSIONS
Dose and administration methods both affect the treatment outcomes. So the drug sensitivity test in vitro dose provide clinical therapy with the optimal dose and administration regimen.
Zhang
Sulin
S
Department of Otolaryngology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.
Kong
Weijia
W
D000970:Antineoplastic Agents / Q000494:pharmacology*; D016190:Carboplatin / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy / Q000473:pathology; D017239:Paclitaxel / Q000494:pharmacology
D000970:Antineoplastic Agents; D016190:Carboplatin; D017239:Paclitaxel; D005472:Fluorouracil