title: Unraveling the spatial distribution of immunoglobulins, enzymes, and polyelectrolytes within layer-by-layer self-assembled multilayers. Ellipsometric studies.
abstract: The ellipsometric characterization of a layer-by-layer electrostatically self-assembled multilayer of polyphenol oxidase and alkaline phosphatase with the polycation poly(dimethyldiallylammonium chloride) built on an immunologic layer formed by immunoglobulin G (IgG) and glucose oxidase-conjugated anti-IgG (IgG-GOD) on glassy carbon is reported. The step-by-step evolution of the psi-Delta ellipsometric angles was followed during film growth. Two optical models, named the three-layer film model and reorganization film model, were employed and found suitable for ellipsometric data interpretation. A comparative analysis of film optical properties, film thickness, and ellipsometric mass assessed from both models is also presented.
authors.affiliation: INFIQC-Departamento de Físicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, Argentina. ferreyra@mail.fcq.unc.edu.ar
authors.forename: N F
authors.initials: NF
authors.lastname: Ferreyra
authors.forename: E S
authors.initials: ES
authors.lastname: Forzani
authors.forename: M
authors.initials: M
authors.lastname: López Teijelo
authors.forename: L
authors.initials: L
authors.lastname: Coche-Guérente
authors.forename: P
authors.initials: P
authors.lastname: Labbé
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000378:metabolism; D003198:Computer Simulation; D004573:Electrolytes / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology; D008954:Models, Biological
chemical_list: D004573:Electrolytes; D007074:Immunoglobulin G; D005949:Glucose Oxidase; D000469:Alkaline Phosphatase