title: Analysis of carbamazepine and its active metabolite, carbamazepine-10,11-epoxide, in human plasma using high-performance liquid chromatography.
abstract: A sensitive method based on high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection was developed for the determination of carbamazepine (CBZ) and one of its active metabolites, carbamazepine-10,11-epoxide (CBZ-E) in human plasma. CBZ, CBZ-E and the internal standard (IS) 10,11-dihydrocarbamazepine were extracted from human plasma into methyl tert-butyl ether. CBZ, CBZ-E and the IS were successfully separated on an RP C18 column with a mobile phase of acetonitrile:methanol:water (18:19:63, v/v/v) and monitored via UV detection at 210 nm. The calibration curves were linear over the concentration ranges of 0.01-10 microg/mL for CBZ and 0.005-5 microg/mL for CBZ-E in human plasma, respectively. The method displayed excellent sensitivity, precision and accuracy, and was successfully applied to the quantification of CBZ and CBZ-E in human plasma after oral administration of a single 200 mg CBZ CR tablet. This method is suitable for bioequivalence studies following single doses given to healthy volunteers.
authors.affiliation: Laboratory of Excellency for Drug and Gene Delivery, College of Pharmacy, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul, 151-742, South Korea.
authors.forename: Eun kyung
authors.initials: Ek
authors.lastname: Oh
authors.forename: Eunmi
authors.initials: E
authors.lastname: Ban
authors.forename: Jong Soo
authors.initials: JS
authors.lastname: Woo
authors.forename: Chong-Kook
authors.initials: CK
authors.lastname: Kim
mesh_terms: D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004852:Epoxy Compounds / Q000097:blood* / Q000737:chemistry*; D006801:Humans
chemical_list: D004852:Epoxy Compounds; D002220:Carbamazepine