[The potential of 1,1'-hexamethylenebis [3-(3,5-dichloro-4-pyridyl)] urea to modify genotoxic actions of chemical mutagens in various test-systems].
The main tasks of this investigation were to investigate the potential genotoxic effect of 1,1'-hexamethylenebis [3-(3,5-dichloro-4-pyridyl)] urea and to analyze its capacity to induce adaptive response (AR) against chemical mutagens in various test-systems. Microbiological, cytogenetical and biochemical end-points were used. The sensitivity of test systems can be arranged as followed: human lymphocyte cultures > Chlamydomonas reinhardtii > Hordeum vulgare. It was obtained that HMPU can induce oxidative stress in Chlamydomonas reinhardtii cells (7.5 x 10(-4) mol/l) and at appropriate experimental conditions can trigger an AR against chemical mutagens in Hordeum vulgare (7.5 x 10(-3) mol/l) and human lymphocytes (10(-5), 10(-6) mol/l). The extent of the AR induction was closely connected with the increase of the inter-treatment time between the conditioning concentration of HMPU and the challenge concentration of chemical mutagens.
Chankova
S G
SG
Ionova
P A
PA
Gateva
S P
SP
Iovchev
G
G
Stergios
M L
ML
Mekhandzhiev
A D
AD
Shevchenko
V A
VA
D000222:Adaptation, Physiological / Q000187:drug effects*; D000375:Aging / Q000187:drug effects*; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000166:cytology / Q000187:drug effects; D004247:DNA / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D001467:Hordeum / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D014508:Urea / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000633:toxicity
C514807:1,1'-hexamethylenebis (3-(3,5-dichloro-4-pyridyl))urea; D009153:Mutagens; D014508:Urea; D004247:DNA