title: Role for nonstructural protein 1 of severe acute respiratory syndrome coronavirus in chemokine dysregulation.
abstract: Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a novel coronavirus. Since its associated morbidity and mortality have been postulated to be due to immune dysregulation, we investigated which of the viral proteins is responsible for chemokine overexpression. To delineate the viral and cellular factor interactions, the role of four SARS coronavirus proteins, including nonstructural protein 1 (nsp-1), nsp-5, envelope, and membrane, were examined in terms of cytokine induction. Our results showed that the SARS coronavirus nsp-1 plays an important role in CCL5, CXCL10, and CCL3 expression in human lung epithelial cells via the activation of NF-kappaB.
authors.affiliation: Department of Paediatrics and Adolescent Medicine, The University of Hong Kong, Pokfulam, Hong Kong, Special Administrative Region, China.
authors.forename: Anna H Y
authors.initials: AH
authors.lastname: Law
authors.forename: Davy C W
authors.initials: DC
authors.lastname: Lee
authors.forename: Benny K W
authors.initials: BK
authors.lastname: Cheung
authors.forename: Howard C H
authors.initials: HC
authors.lastname: Yim
authors.forename: Allan S Y
authors.initials: AS
authors.lastname: Lau
mesh_terms: D054405:Chemokine CCL3; D018946:Chemokine CCL5; D054357:Chemokine CXCL10; D018925:Chemokines / Q000235:genetics* / Q000378:metabolism; D019742:Chemokines, CC / Q000235:genetics / Q000378:metabolism; D019743:Chemokines, CXC / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology / Q000821:virology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000378:metabolism / Q000472:pathogenicity*; D014759:Viral Envelope Proteins / Q000502:physiology; D014763:Viral Matrix Proteins / Q000502:physiology; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: C515429:CCL3 protein, human; C483024:CCL5 protein, human; C114753:CXCL10 protein, human; D054405:Chemokine CCL3; D018946:Chemokine CCL5; D054357:Chemokine CXCL10; D018925:Chemokines; D019742:Chemokines, CC; D019743:Chemokines, CXC; D016328:NF-kappa B; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins