title: Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia.
abstract: Silene latifolia is a model dioecious plant with heteromorphic sex chromosomes. The Y chromosome is the largest in this species. Theoretical models propose an accumulation of repetitive DNA sequences in non-recombining parts of the Y chromosome. In this study, we isolated a BAC7H5 clone preferentially hybridizing to the Y chromosome of S. latifolia. Sequence analysis revealed that this BAC7H5 contains part of the chloroplast genome, indicating that these chloroplast sequences have accumulated on the Y chromosome and also may contribute to its large size. We constructed Y chromosome- and X chromosome-specific libraries and screened them to find Y- and/or X-linked copies of chloroplast sequences. Sequence analysis revealed higher divergence of a non-genic region of the chloroplast sequences located on the Y chromosome while genic regions tested showed only very low (max 0.9%) divergence from their chloroplast homologues.
authors.affiliation: Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ-612 65, Brno, Czech Republic. kejnovsk@ibp.cz
authors.forename: Eduard
authors.initials: E
authors.lastname: Kejnovsky
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Kubat
authors.forename: Roman
authors.initials: R
authors.lastname: Hobza
authors.forename: Martina
authors.initials: M
authors.lastname: Lengerova
authors.forename: Shusei
authors.initials: S
authors.lastname: Sato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tabata
authors.forename: Kiichi
authors.initials: K
authors.lastname: Fukui
authors.forename: Sachihiro
authors.initials: S
authors.lastname: Matsunaga
authors.forename: Boris
authors.initials: B
authors.lastname: Vyskot
mesh_terms: D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018742:DNA, Chloroplast / Q000235:genetics*; D019143:Evolution, Molecular; D017404:In Situ Hybridization, Fluorescence; D008957:Models, Genetic; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D029751:Silene / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018742:DNA, Chloroplast