Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64A.
Viruses NY-2A and AR158, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella NC64A. The 368,683-bp genome of NY-2A and the 344,690-bp genome of AR158 are the two largest chlorella virus genomes sequenced to date; NY-2A contains 404 putative protein-encoding and 7 tRNA-encoding genes and AR158 contains 360 putative protein-encoding and 6 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Two of the NY-2A genes encode inteins, the large subunit of ribonucleotide reductase and a superfamily II helicase. These are the first inteins to be detected in the chlorella viruses. Approximately 40% of the viral gene products resemble entries in the public databases, including some that are unexpected for a virus. These include GDP-d-mannose dehydratase, fucose synthase, aspartate transcarbamylase, Ca(++) transporting ATPase and ubiquitin. Comparison of NY-2A and AR158 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that 85% of the genes are present in all three viruses.
Fitzgerald
Lisa A
LA
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Graves
Michael V
MV
Li
Xiao
X
Feldblyum
Tamara
T
Nierman
William C
WC
Van Etten
James L
JL
D000595:Amino Acid Sequence; D001118:Argentina; D001482:Base Composition; D002708:Chlorella / Q000821:virology*; D011088:DNA Ligases / Q000235:genetics; D005618:Fresh Water / Q000821:virology; D005814:Genes, Viral / Q000502:physiology; D016679:Genome, Viral / Q000235:genetics*; D047668:Inteins / Q000235:genetics; D008969:Molecular Sequence Data; D009518:New York; D016366:Open Reading Frames / Q000235:genetics; D017862:Phycodnaviridae / Q000145:classification / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins; C110456:Chlorella virus DNA ligase; D011088:DNA Ligases