[Study on quality control and fingerprint of Chinese traditional medicine Yinhuang oral liquid].
The chromatographic fingerprint of Chinese traditional medicine, Yinhuang Oral Liquid together with its original materials Radix Scutellariae and Flos lonicera was established by high performance liquid chromatography (HPLC). At the same time, baicalin and chlorogenic acid in the Yinhuang Oral Liquid were also quantitatively determined. The analysis was performed on a Lichrospher C18 column (250 mm x 4.6 mm i. d. , 5 microm) and with gradient elution. The optimum conditions were explored. The mobile phase A was methanol and the mobile phase B was 0.1% H3PO4 aqueous solution. The wavelength of 254 nm was selected for detection. Finally, the data of fingerprints of 6 samples of Yinhuang Oral Liquid were processed with two different mathematic methods. This proposed method demonstrated good stability for the 6 samples of Yinhuang Oral Liquid. In the comparison of the common peaks in chromatogram of Yinhuang Oral Liquid with those of crude herbs, most of them could be matched well.
Wang
Licong
L
School of Chemical and Material Engineering, Southern Yangze University, Wuxi 214036, China. wanglicong0828@yahoo.com.cn
Cao
Yuhua
Y
Xu
Honglan
H
Ye
Jiannong
J
D002726:Chlorogenic Acid / Q000032:analysis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000592:standards; D005419:Flavonoids / Q000032:analysis; D029001:Herbal Medicine / Q000592:standards; D031239:Lonicera / Q000737:chemistry*; D008516:Medicine, Chinese Traditional / Q000592:standards*; D011786:Quality Control; D031364:Scutellaria baicalensis / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D002726:Chlorogenic Acid; C038044:baicalin