title: Administration of prednisolone phosphate-liposomes reduces wound contraction in a rat partial-thickness wound model.
abstract: Macrophages play an important role in the inflammatory phase of wound healing and their activity regulates fibroblasts and keratinocytes. Modulation of macrophage function may result in improvement of the wound healing process. Prednisolone phosphate (PLP) encapsulated into liposomes was administered to partial-thickness wounds in rats. A single dose of 75 microg/kg, applied directly after wounding, resulted in up to a 30% reduction of wound contraction at 28 days after wounding. This effect could not be achieved in the group that was administered free PLP or liposomes containing phosphate-buffered saline to the wound. The number of myofibroblasts was up to 50% lower in wounds treated with the liposomal PLP at 4 days after wounding. The number of macrophages present in the wounds was not statistically different between groups. Most probably, the production of cytokines and growth factors by macrophages is altered after phagocytosing the liposomes, resulting in reduced wound contraction.
authors.affiliation: Department of Molecular Cell Biology and Immunology, Medical Faculty, Vrije Universiteit Medical Centre (VUMC), Amsterdam, the Netherlands. cd.richters@vumc.nl
authors.forename: Cornelia D
authors.initials: CD
authors.lastname: Richters
authors.forename: Nanne J
authors.initials: NJ
authors.lastname: Paauw
authors.forename: Ilvy
authors.initials: I
authors.lastname: Mayen
authors.forename: Louis
authors.initials: L
authors.lastname: van Bloois
authors.forename: Josbert M
authors.initials: JM
authors.lastname: Metselaer
authors.forename: Gert
authors.initials: G
authors.lastname: Storm
authors.forename: John S
authors.initials: JS
authors.lastname: du Pont
authors.forename: Matthias J
authors.initials: MJ
authors.lastname: Hoekstra
authors.forename: Robert W
authors.initials: RW
authors.lastname: Kreis
authors.forename: Eduard W A
authors.initials: EW
authors.lastname: Kamperdijk
mesh_terms: D000287:Administration, Topical; D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D007150:Immunohistochemistry; D008081:Liposomes; D008297:Male; D011239:Prednisolone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D008081:Liposomes; C009022:prednisolone phosphate; D011239:Prednisolone