[Methylation status of FHIT gene in plasma and expression of FHIT gene in cancer tissue of cervical cancer patients].
OBJECTIVE
To explore the methylation status of 5' CpG island of fragile histidine triad (FHIT) gene in plasma and the expression of FHIT protein in cancer tissue of cervical cancer patients.


METHODS
Methylation-specific PCR (MS-PCR) was employed to examine methylation of FHIT gene in 151 plasma samples before treatment. The immunohistochemistry was used to the expression of FHIT protein in cancer tissues.


RESULTS
CpG island methylation of FHIT was detected in 31.13% of plasma samples. The expression of FHIT protein was decreased or discarded in 59.60% of cervical cancer tissues. Among them 47.78% was included in methylation positive samples.


CONCLUSIONS
CpG island methylation of FHIT gene in plasma plays an important role on cervical cancer, which results in decreased expression of FHIT protein. It can be used to diagnose and evaluate the effect of treatment to cervical cancers.
Ren
Chen-chun
CC
Department of Genetics, the Central Hospital of Gynecology and Obstetric, Tianjin, 300052 PR China.
Miao
Xu-hong
XH
Yang
Bin
B
Zhao
Lei
L
Sun
Rui
R
Song
Wen-qin
WQ
D017766:Acid Anhydride Hydrolases / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D000368:Aged; D019175:DNA Methylation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase