[Species identification by multiplex amplifying mtDNA-HV I, HV II and cytb regions].
OBJECTIVE
To evaluate the method for species identification by multiplex amplification of mtDNA-HV I, HV II and cytb regions.


METHODS
The bloodstains or muscular tissues of human and 16 kinds of animals were collected. DNA were quantified after extraction. Three pairs of primers were mixed in the same reaction and the PCR amplification products were analyzed by PAGE and silver staining.


RESULTS
The presence of three bands (278 bp, 358 bp, 425 bp) indicated the samples were from human, while only one band indicated nonhuman origin. The position of the animal's only band is different from the band (358 bp) of human.


CONCLUSIONS
The method of species identification by multiplex amplifying mtDNA-HV I, HV II and cytb regions is simple and sensitive. It is of use for forensic identification.
Wang
Chuang
C
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Yang
Zhi-hui
ZH
Liang
Wei-bo
WB
Zhou
Bin
B
Zhang
Lin
L
D000818:Animals; D002415:Cats; D002417:Cattle; D045303:Cytochromes b / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D004285:Dogs; D005554:Forensic Medicine; D006801:Humans; D051379:Mice; D021141:Nucleic Acid Amplification Techniques; D011817:Rabbits; D013045:Species Specificity
D017931:DNA Primers; D004272:DNA, Mitochondrial; D045303:Cytochromes b