title: Multilocus enzyme electrophoresis supports speciation within the Anopheles nili group of malaria vectors in Cameroon.
abstract: Multilocus enzyme analysis of the genetic variability and population structure was conducted among three malaria vector species of the Anopheles nili group in Cameroon: An. nili, An. carnevalei, and the recently described An. ovengensis. We detected species-specific alleles and large differences in shared allele frequencies at six of nine loci (e.g., PGM, GOT1, IDH1, IDH2, PGI, and alpha-GPD). This non-random distribution of alleles leads to high and significant values of differentiation indexes (0.569 < Fst < 0.874, P < 10(-4)). These results fully agree with standard morphologic descriptions, and therefore provide further support for recent taxonomic classification within the An. nili group.
authors.affiliation: Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Yaoundé, Cameroon. hpaawono@yahoo.fr
authors.forename: Herman P
authors.initials: HP
authors.lastname: Awono-Ambene
authors.forename: Frédéric
authors.initials: F
authors.lastname: Simard
authors.forename: Christophe
authors.initials: C
authors.lastname: Antonio-Nkondjio
authors.forename: Anna
authors.initials: A
authors.lastname: Cohuet
authors.forename: Pierre
authors.initials: P
authors.lastname: Kengne
authors.forename: Didier
authors.initials: D
authors.lastname: Fontenille
mesh_terms: D000483:Alleles; D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D002163:Cameroon; D004275:DNA, Ribosomal / Q000737:chemistry; D004592:Electrophoresis, Starch Gel; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D006801:Humans; D007303:Insect Vectors / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics; D008288:Malaria / Q000635:transmission*; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D004275:DNA, Ribosomal; D007527:Isoenzymes