Enhanced expression levels of IL-31 correlate with IL-4 and IL-13 in atopic and allergic contact dermatitis.
BACKGROUND
IL-31 is produced by activated T lymphocytes, preferentially by TH2 cells. Transgenic mice overexpressing IL-31 have a phenotype resembling allergic dermatitis in human subjects.


OBJECTIVE
We sought to evaluate the potential importance of IL-31 in the pathogenesis of human T cell-mediated skin diseases.


METHODS
We analyzed total RNA taken from 149 skin biopsy specimens from patients with atopic dermatitis (AD), allergic contact dermatitis (ACD), or psoriasis in comparison with specimens taken from patients with healthy skin (n = 13) by using quantitative real-time PCR for the expression of TH1/TH2 cytokines.


RESULTS
We found statistically increased mRNA levels of IL-31 in biopsy specimens taken from patients with AD, irrespective of the severity of the disease and serum IgE levels. Moreover, IL-31 mRNA levels were strongly increased in many biopsy specimens taken from patients with ACD. However, no increased transcription of IL-31 could be detected in biopsy specimens taken from psoriatic plaques. A comparison of mRNA levels of IL-31 with TH1 or TH2 cytokines demonstrates a correlation of the expression of IL-31 with IL-4 and IL-13 but not with IFN-gamma. No significant increase of IL-31 receptor mRNA could be detected in any disease, whereas the second receptor subunit of IL-31, the oncostatin M receptor, seems to be enhanced transcribed in patients with psoriasis.


CONCLUSIONS
IL-31 expression is not only increased in patients with AD but also in those with ACD, 2 pruritic skin disorders. In both types of eczema, expression of IL-31 is associated with the expression of the TH2 cytokines IL-4 and IL-13.


CONCLUSIONS
IL-31 might contribute not only to the development of AD but also to ACD-provoked skin inflammation.
Neis
Mark M
MM
Department of Dermatology and Allergology, University Hospital RWTH Aachen, Germany.
Peters
Bettina
B
Dreuw
Alexandra
A
Wenzel
Joerg
J
Bieber
Thomas
T
Mauch
Cornelia
C
Krieg
Thomas
T
Stanzel
Sven
S
Heinrich
Peter C
PC
Merk
Hans F
HF
Bosio
Andreas
A
Baron
Jens M
JM
Hermanns
Heike M
HM
D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D018793:Interleukin-13 / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis*; D007378:Interleukins / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014158:Transcription, Genetic
C502187:IL31 protein, human; D018793:Interleukin-13; D007378:Interleukins; D012333:RNA, Messenger; D015847:Interleukin-4; D007073:Immunoglobulin E