Systematic analysis of sequence variability of the endothelin-1 gene: a prerequisite for association studies.
We analyzed allele frequencies and pairwise linkage disequilibria of 13 variants in the EDN1 gene of 298 young males, the majority of German ancestry. Our analysis comprises all common variants in the five exons and flanking intronic regions, as well as known polymorphisms in the promoter sequence. In addition to previously analyzed polymorphisms, our haplotype reconstruction included five recently described variants and was done by using three different algorithms to allow inference of result stability. More than 30 haplotypes were predicted. All haplotypes with frequencies > or = 1% were inferred by all three methods and can be described by seven haplotype tagging single-nucleotide polymorphisms (htSNPs), reducing the genotyping load to 65%. Three of these haplotypes with frequencies of about 11%, 9%, and 4% had been mistaken for one haplotype in the previous analysis, which included only six polymorphisms, some of them not being htSNPs. Systematic analysis of sequence variability and comprehensive haplotype analysis of the EDN1 gene determined a substantial part of its genetic variability for further association studies and helped to reduce the genotyping load for common phenotypes.
Diefenbach
Konstanze
K
Institute of Clinical Pharmacology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Arjomand-Nahad
Farhad
F
Meisel
Christian
C
Fietze
Ingo
I
Stangl
Karl
K
Roots
Ivar
I
Köpke
Karla
K
D000328:Adult; D019332:Endothelin-1 / Q000235:genetics*; D014644:Genetic Variation; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D017422:Sequence Analysis, DNA
D019332:Endothelin-1