Molecular method for identification of Rickettsia species in clinical and environmental samples.
We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens.
Jado
Isabel
I
Laboratorio de Espiroquetas y Patógenos Especiales, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220-Majadahonda, Madrid, Spain.
Escudero
Raquel
R
Gil
Horacio
H
Jiménez-Alonso
María Isabel
MI
Sousa
Rita
R
García-Pérez
Ana L
AL
Rodríguez-Vargas
Manuela
M
Lobo
Bruno
B
Anda
Pedro
P
D000818:Animals; D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012281:Rickettsia / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000175:diagnosis / Q000382:microbiology*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D005423:Siphonaptera / Q000382:microbiology; D013987:Ticks / Q000382:microbiology; D014871:Water Microbiology
D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer