title: Electrochemical studies of (-)-epigallocatechin gallate and its interaction with DNA.
abstract: In this paper, an electrochemical investigation of (-)-epigallocatechin gallate (EGCG) and its interaction with DNA is presented. Via an electrochemical approach assisted by ultraviolet-visible (UV-Vis) spectroscopy, we propose that EGCG can intercalate into DNA strands forming a nonelectroactive complex, which results in the decrease of the anodic peak current of EGCG. Meanwhile, an electrochemical study with the DNA-Cu(II)-EGCG system shows that damage to DNA can be recognized electrochemically via the increase in the anodic peak current resulting from the oxidation of guanine and adenine bases. The damage can also be recognized spectrophotometrically via an increase in the 260 nm absorption band. In addition, it was found that EGCG is able to discriminate dsDNA from ssDNA, making a potential electrochemical indicator for the detection of DNA hybridization events. A rapid and convenient method of detecting EGCG was also developed in this work.
authors.affiliation: Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zheng
authors.forename: Anyue
authors.initials: A
authors.lastname: Chen
authors.forename: Tomonori
authors.initials: T
authors.lastname: Hoshi
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Anzai
authors.forename: Genxi
authors.initials: G
authors.lastname: Li
mesh_terms: D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D015394:Molecular Structure
chemical_list: D002392:Catechin; D004247:DNA; C045651:epigallocatechin gallate