Specific integrin subunits in bovine oocytes, including novel sequences for alpha 6 and beta 3 subunits.
Integrins facilitate attachment of cells to the extra-cellular matrix, often binding the arginine-glycine-aspartic acid tri-peptide motif, thus facilitating cell migration, mediating cell-cell adhesion, linking the extracellular matrix (ECM) with cytoskeletal elements, and acting as signaling molecules. Adhesion activates signaling mechanisms that regulate integrin function, cytoskeletal assembly, cell behavior, and protein synthesis. Immunofluorescence was used to determine the presence of integrin alpha and beta subunits on the surface of bovine oocytes using a panel of monoclonal antibodies (mAbs) specific for alphaL, alphaM, alphaX, alphaV, alpha2, alpha4, alpha6, beta1, beta2, and beta3 antigens, with multiple antibodies for each subunit. Confocal microscopy indicated the presence of alphaV, alpha6, alpha4, alpha2, ss1, and ss3 integrin subunits on the plasma membrane of bovine oocytes. The presence of these subunits was verified by RT-PCR analysis using primers designed based on known gene sequences of bovine integrin subunits, or by using sequence information using bovine expressed sequence tags (EST) compared with known human and murine integrin subunit gene sequence information. Previously unpublished sequence information for bovine alpha6 and beta3 integrins was determined. The presence of these integrin subunits on the bovine oocyte vitelline membrane supports the hypothesis that sperm-oocyte interactions in the bovine are mediated by integrins.
Pate
Barry J
BJ
Department of Animal, Dairy, and Veterinary Sciences and Center for Integrated BioSystems, Utah State University, Logan, Utah 84322-4815, USA.
White
Kenneth L
KL
Winger
Quinton A
QA
Rickords
Lee F
LF
Aston
Kenneth I
KI
Sessons
Benjamin R
BR
Li
Guang-Peng
GP
Campbell
Kenneth D
KD
Weimer
Bart
B
Bunch
Thomas D
TD
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D039503:Integrin alpha6 / Q000378:metabolism*; D039661:Integrin beta3 / Q000378:metabolism*; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D021122:Protein Subunits / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA
D039503:Integrin alpha6; D039661:Integrin beta3; D021122:Protein Subunits; D012333:RNA, Messenger