title: Inhibitory effect of mizoribine on matrix metalloproteinase-1 production in synovial fibroblasts and THP-1 macrophages.
abstract: To investigate the mechanism of antirheumatic action of mizoribine (MZR), we examined the expression of matrix metalloproteinase-1 (MMP-1) and MMP-3 utilizing THP-1 derived macrophage-like cells (THP-1 macrophages) and human synovial fibroblasts (SFs). The cells were respectively stimulated with lipopolysaccharide (LPS) and interleukin-1beta in the presence or absence of MZR in vitro. The concentrations of MMP-1 and MMP-3 in the supernatant were measured by enzyme-linked immunosorbent assay. The secretion of MMP-1 from SFs, as well as THP-1 macrophages, was inhibited by MZR in a dose-dependent manner. Furthermore, a quantitative real-time polymerase chain reaction revealed that MZR decreased the expression of MMP-1 messenger RNA. These findings may be an explanation for the clinical effect of MZR in patients with rheumatoid arthritis.
authors.affiliation: Department of Rheumatology and Internal Medicine, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
authors.forename: Binbin
authors.initials: B
authors.lastname: Zhong
authors.forename: Michiko
authors.initials: M
authors.lastname: Tajima
authors.forename: Hidenari
authors.initials: H
authors.lastname: Takahara
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nochi
authors.forename: Koichi
authors.initials: K
authors.lastname: Tamoto
authors.forename: Naoto
authors.initials: N
authors.lastname: Tamura
authors.forename: Shigeto
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Tamura
authors.forename: Makoto
authors.initials: M
authors.lastname: Ikeda
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Akimoto
authors.forename: Shinichi
authors.initials: S
authors.lastname: Yoshino
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hashimoto