[Determination of egg-yolk phosphatidylcholine by normal-phase high performance liquid chromatography with evaporative light scattering detection].
A method for the determination of egg-yolk phosphatidylcholine by normal-phase high performance liquid chromatography (NP-HPLC) with evaporative light scattering detection (ELSD) was developed. Yolk phosphatidylcholine was successfully separated on a Nova-Pak Silica 60A column (3.9 mm i.d. x 150 mm, 4 microm), using hexane-isopropanol-water containing 3% acetic acid (35: 65: 8, v/v/v) as mobile phase at a flow rate of 1.0 mL/min. The drift tube temperature and air carrier gas pressure of the ELSD were set at 50 degrees C and 350 kPa, respectively. The linear detection range was 0.16 - 1.61 g/L (r2 = 0.997 9) and the limit of detection (LOD) was 0.64 microg with excellent relative standard deviation (RSD) of 3.2% (n = 5). The recovery was in the range of 98.2% - 128.2%. The results indicated that this method is accurate, rapid, simple and reproducible, and thus suitable for the analysis of egg-yolk phosphatidylcholine in real samples.
Gong
Yan
Y
Department of Science, Beijing University of Chemical Technology, Beijing 100029, China.
Wang
Qiao
Q
Yang
Yi
Y
Ding
Mingyu
M