title: Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions.
abstract: The lateral diffusion coefficients of a BODIPY tail-labeled lipid in two model systems, namely, free-standing giant unilamellar vesicles (GUVs) and supported phospholipid bilayers (SPBs), were determined by fluorescence correlation spectroscopy (FCS) using the Z-scan approach. For the first time, the performed measurements on 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) bilayers maintain exactly the same experimental conditions for both systems, which allows for a quantitative comparison of lipid diffusion in these two commonly used model membranes. The results obtained revealed that the lipid mobility in free-standing bilayers (D=7.8+/-0.8 microm2 s-1) is significantly higher than in the bilayer created on the solid support (mica) (D=3.1+/-0.3 microm2 s-1).
authors.affiliation: J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, 18223 Prague 8, Czech Republic.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Przybylo
authors.forename: Jan
authors.initials: J
authors.lastname: Sýkora
authors.forename: Jana
authors.initials: J
authors.lastname: Humpolíckova
authors.forename: Ales
authors.initials: A
authors.lastname: Benda
authors.forename: Anna
authors.initials: A
authors.lastname: Zan
authors.forename: Martin
authors.initials: M
authors.lastname: Hof
mesh_terms: D004058:Diffusion; D008051:Lipid Bilayers / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D013997:Time Factors
chemical_list: D008051:Lipid Bilayers; D010713:Phosphatidylcholines; C017251:1,2-oleoylphosphatidylcholine