Proteomics analysis reveals insight into the mechanism of H-Ras-mediated transformation.
We implemented a proteomics approach to the systematical analysis of the alterations in the proteome of NIH3T3 cells transformed by oncogenic H-RasV12. Forty-four proteins associated with Ras-mediated transformation have been identified, and 28 proteins were not previously reported. RT-PCR analysis showed that approximately 44% of target proteins identified showed concomitant changes in mRNA abundance. A principal finding was the up-regulation of gankyrin, which was the first evidence to show that gankyrin pathway was implicated in Ras-activated transformation.
Jin
Bao-Feng
BF
Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing 100850, China.
He
Kun
K
Wang
Hong-Xia
HX
Bai
Bing
B
Zhou
Tao
T
Li
Hui-Yan
HY
Man
Jiang-Hong
JH
Liu
Bing-Yu
BY
Gong
Wei-Li
WL
Wang
Jie
J
Li
Ai-Ling
AL
Zhang
Xue-Min
XM
D000818:Animals; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D016000:Cluster Analysis; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D015689:Oncogene Protein p21(ras) / Q000235:genetics*; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D040901:Proteomics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection
D020543:Proteome; D012333:RNA, Messenger; D015689:Oncogene Protein p21(ras)