Pineapple [Ananas comosus (L.) Merr].
A procedure for pineapple [Ananas comosus (L.) Merr.] genetic transformation is described, which involves temporary immersion bioreactors (TIB) for selection of transgenic plants. Success in the production of transgenic pineapple plants combines tissue culture factors. Firstly, the use of regenerable pineapple callus as starting material for transformation whose cells shown to be competent for Agrobacterium infection. Secondly, the used of filtered callus, resulting in homogeneously sized clusters, thereby increasing the contact between the cell surfaces and A. tumefaciens and releasing phenolic compounds which induce Agrobacterium virulence. Thirdly, regeneration of primary plants without selection pressure, that allowing a massive production of putative transgenic pineapples. Finally, we support that TIB technology is a powerful system to recover nonchimera transgenic plants by micropropagation with the use of an adequate selection agent.
Yabor
Lourdes
L
Bioplant Center, University of Ciego de Avila, Carretera a Morón km 9, CP 69450, Ciego de Avila, Cuba.
Espinosa
Patricia
P
Arencibia
Ariel D
AD
Lorenzo
José C
JC
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D031230:Ananas / Q000235:genetics* / Q000254:growth & development; D019149:Bioreactors; D015139:Blotting, Southern; D003470:Culture Media; D005822:Genetic Vectors; D036341:Intercellular Signaling Peptides and Proteins; D009942:Organometallic Compounds; D010455:Peptides; D030821:Plants, Genetically Modified / Q000254:growth & development; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
D003470:Culture Media; D036341:Intercellular Signaling Peptides and Proteins; D009942:Organometallic Compounds; C074094:PC 1038; D010455:Peptides