Detection of human papillomavirus in cervical cell specimens by hybrid capture and PCR with different primers.
The purpose of this study was to compare hybrid capture assay with PCRs using different primers for the L1, E6-E7 regions for the detection of human papillomavirus (HPV) genome. One hundred twenty-five cervical smears with normal (n=42) and abnormal (n=83) cytology were investigated. Those at high-risk for HPV were studied by hybridization antibody capture assay and PCR with the pU-1M/pU-2R primers. Target DNA from the HPV L1 region was amplified by SPF10 primer set and home-PCR with MY09/MY11 primers. The presence of HPV DNA in cervical smears was detected by SPF10 (in 72% of cases), MY09/MY11 (58%), hybrid capture (55%) and pU-1M/pU-2R (39%). Results obtained with the SPF10 and MY09/MY11 consensus primer sets as well as hybrid capture and pU-1M/pU-2R specific for high-risk types differed significantly (chi2, P<0.0005). The correlation between assays with the use of SPF10 and MY09/MY11 was 86% and between hybrid capture and the pU-1M/pU2R technique--78%. In 49% of samples HPV DNA was detected by the four methods, whereas in 12% only by the SPF10 primers. The most sensitive technique was found to be PCR with the use of SPF10 primers, while the most specific--the MY09/11 PCR method. It seems that home-PCR with MY09/MY11 primers could be applied in screening tests.
Szostek
Slawa
S
Jagiellonian University Medical College, Chair of Microbiology, Department of Virology, Kraków, Poland. sszostek@cm-uj.krakow.pl
Klimek
Malgorzata
M
Zawilinska
Barbara
B
Rys
Janusz
J
Kope
Jolanta
J
Daszkiewic
Ewa
E
D000818:Animals; D000974:Antibodies, Antinuclear; D002584:Cervix Uteri / Q000166:cytology / Q000821:virology*; D017931:DNA Primers; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D015347:RNA Probes; D012680:Sensitivity and Specificity; D014626:Vaginal Smears
D000974:Antibodies, Antinuclear; D017931:DNA Primers; D015344:DNA Probes, HPV; D004279:DNA, Viral; D015347:RNA Probes