title: Molecular method for identification of Rickettsia species in clinical and environmental samples.
abstract: We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens.
authors.affiliation: Laboratorio de Espiroquetas y Patógenos Especiales, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220-Majadahonda, Madrid, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Jado
authors.forename: Raquel
authors.initials: R
authors.lastname: Escudero
authors.forename: Horacio
authors.initials: H
authors.lastname: Gil
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Jiménez-Alonso
authors.forename: Rita
authors.initials: R
authors.lastname: Sousa
authors.forename: Ana L
authors.initials: AL
authors.lastname: García-Pérez
authors.forename: Manuela
authors.initials: M
authors.lastname: Rodríguez-Vargas
authors.forename: Bruno
authors.initials: B
authors.lastname: Lobo
authors.forename: Pedro
authors.initials: P
authors.lastname: Anda
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012281:Rickettsia / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000175:diagnosis / Q000382:microbiology*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D005423:Siphonaptera / Q000382:microbiology; D013987:Ticks / Q000382:microbiology; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer