title: SECRET AGENT, an Arabidopsis thaliana O-GlcNAc transferase, modifies the Plum pox virus capsid protein.
abstract: The capsid protein of Plum pox virus (PPV-CP) is modified with O-linked GlcNAc (O-GlcNAc). While Arabidopsis has two O-GlcNAc transferases, SECRET AGENT (SEC) and SPINDLY (SPY), previous work suggests that SEC modifies PPV-CP and that the modification plays a role in the infection process. Here, we show that when co-expressed in Escherichia coli SEC modifies PPV-CP. Deletion mapping and site-directed mutagenesis identified three threonine and a serine located near the N-terminus of PPV-CP that are modified by SEC. Two of these threonines have recently been shown to be modified in virus from plants suggesting that SEC has the same specificity in plants and E. coli.
authors.affiliation: Department of Plant Biology, University of Minnesota, 1445 Gortner Avenue, 250 Biological Sciences Center, St. Paul, MN 55108, USA.
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Scott
authors.forename: Lynn M
authors.initials: LM
authors.lastname: Hartweck
authors.forename: José
authors.initials: J
authors.lastname: de Jesús Pérez
authors.forename: Dinghu
authors.initials: D
authors.lastname: Chen
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: García
authors.forename: Neil E
authors.initials: NE
authors.lastname: Olszewski
mesh_terms: D019943:Amino Acid Substitution; D017360:Arabidopsis / Q000201:enzymology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics; D006031:Glycosylation; D016297:Mutagenesis, Site-Directed; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D017801:Plum Pox Virus / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D012694:Serine / Q000737:chemistry; D013912:Threonine / Q000737:chemistry
chemical_list: D029681:Arabidopsis Proteins; D036022:Capsid Proteins; D004279:DNA, Viral; D011994:Recombinant Proteins; D013912:Threonine; D012694:Serine; D017351:N-Acetylglucosaminyltransferases; C510668:SECRET AGENT protein, Arabidopsis