[Determination of three constituents in Radix Astragali by HPLC-MS].
OBJECTIVE
To determine calycosin-7-O-beta-D-glucoside, astragaloside IV and formononetin in Radix Astragali and other relative samples by HPLC-MS.


METHODS
HPLC was carried out with Agilent 1100LC/MSD, equipped with Agilent Zorbax SB C18 column (250 mm x 4.6 mm ID, 5 microm) and mass spectrum detector. The mobile phase (CH3CN-H2O) was eluted in gradient mode.


RESULTS
The calibration curves of calycosin-7-O-beta-D-glucoside, astragaloside IV and formononetin were linear in the range of 0.03 - 1.21 microg x mL(-1), 0.35 - 13.86 microg x mL(-1) and 0.38 - 15.22 microg x mL(-1), respectively. These recoveries of samples were from 95% to 105% with RSD less than 1.5%.


CONCLUSIONS
The method was employed to analyse 25 samples of Radix Astragali and other relative samples, including Radix Astragali slice, Radix Astragali Preparata, Hedysarum polybotrys Hand. -Mazz, Astragalus ernestii Comb. The contents of three constituents vary greatly because of the species, place of collection and season of harvesting. This method could apply to evaluate the quality of Radix Astragali and it is simple, sensitive and reliable.
Li
Xiang
X
School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Zhu
Zhen-yu
ZY
Wang
Bin
B
Lou
Zi-yang
ZY
Chai
Yi-feng
YF
D027883:Astragalus Plant / Q000737:chemistry; D027885:Astragalus propinquus / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017753:Ecosystem; D005960:Glucosides / Q000032:analysis*; D007529:Isoflavones / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D012621:Seasons; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014315:Triterpenes / Q000032:analysis*
D005960:Glucosides; D007529:Isoflavones; D012503:Saponins; D014315:Triterpenes; C526426:calycosin-7-O-beta-D-glucoside; C007768:formononetin; C052064:astragaloside A