Induction of acid phosphatase activity during germination of maize (Zea mays) seeds.
Acid phosphatase activity (orthophosphoric-monoester phosphohydrolase, EC 3.1.3.2) increased during the first 24 h of maize (Zea mays) seed germination. The enzyme displayed a pH optimum of 4.5-5.5. Catalytic activity in vitro displayed a linear time course (60 min) and reached its half maximum value at 0.47 mM p-nitrophenyl phosphate (pNPP). Phosphatase activity towards phosphoamino acids was greatest for phosphotyrosine. The phosphatase activity was strongly inhibited by ammonium molybdate, vanadate and NaF and did not require divalent cations for the catalysis. The temperature optimum for pNPP hydrolysis was 37 degrees C. Under the same conditions, no enzyme activity was detected with phytic acid as substrate. Western blotting of total homogenates during seed germination revealed proteins/polypeptides that were phosphorylated on tyrosine residues; a protein of approximately 14 kDa is potentially a major biological substrate for the phosphatase activity. The results presented in this study suggest that the acid phosphatase characterized under the tested conditions is a member of the phosphotyrosine phosphatase family.
Senna
R
R
Departamento de Nutrição Básica e Experimental, Instituto de Nutrição Josué de Castro, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-590, Brazil.
Simonin
V
V
Silva-Neto
M A C
MA
Fialho
E
E
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002413:Cations, Divalent / Q000494:pharmacology; D004789:Enzyme Activation; D018525:Germination / Q000187:drug effects / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008982:Molybdenum / Q000494:pharmacology; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010766:Phosphorylation; D010833:Phytic Acid; D010940:Plant Proteins / Q000378:metabolism*; D012639:Seeds / Q000187:drug effects / Q000201:enzymology*; D012969:Sodium Fluoride / Q000494:pharmacology; D013696:Temperature; D014443:Tyrosine / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology; D003313:Zea mays / Q000187:drug effects / Q000201:enzymology*
D002413:Cations, Divalent; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D010940:Plant Proteins; C008644:nitrophenylphosphate; D014638:Vanadates; D014443:Tyrosine; D010833:Phytic Acid; D008982:Molybdenum; D012969:Sodium Fluoride; D000135:Acid Phosphatase; C022175:ammonium molybdate