Evidence for a tellurite-dependent generation of reactive oxygen species and absence of a tellurite-mediated adaptive response to oxidative stress in cells of Pseudomonas pseudoalcaligenes KF707.
Tellurite (TeO3(2-)) is the most toxic and soluble oxyanion among tellurium (Te) compounds. The effects of the metalloid anion on the oxidative stress response of the obligate aerobe Pseudomonas pseudoalcaligenes KF707 were investigated. Cells treated with sub-lethal concentrations of TeO3(2-) showed neither adaptation to it nor cross-protection against oxidants such as 1,1'-4,4'-bipyridinium dichloride (paraquat, PQ2+), diazenedicarboxylic acid bis-N,N-dimethylamide (diamide), tert-butyl hydroperoxide (tBH) and hydrogen peroxide (H2O2). Notably, TeO3(2-) exerted a synergic effect on the toxicity of these latter oxidants. Tellurite was shown to decrease the cellular content of reduced thiols (RSH) with a consequent increase in the production of reactive oxygen species (ROS) and stimulation of the superoxide dismutase (SOD) activity. However, since the time course of ROS production by TeO3(2) (t1/2 > 30 min) was much slower than that with PQ2+ and/or diamide (t1/2 <or= 10 min), in the former case the SOD activity was poorly activated. We conclude that in P. pseudoalcaligenes KF707 cells: (1) the TeO3(2-) acts as a pro-oxidant by stimulating ROS production; (2) the release of superoxide oxyanions is directly linked to the mechanism of toxicity; (3) TeO3(2-) is unable to induce an adaptive response to oxidative stress.
Tremaroli
Valentina
V
Department of Biology, General Microbiology Unit, Faculty of Sciences, University of Bologna, via Irnerio 42, 40126 Bologna, Italy.
Fedi
Stefano
S
Zannoni
Davide
D
D000222:Adaptation, Physiological / Q000187:drug effects*; D018384:Oxidative Stress; D044205:Pseudomonas pseudoalcaligenes / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013691:Tellurium / Q000494:pharmacology*
D017382:Reactive Oxygen Species; D013691:Tellurium