Flow cytometry as a diagnostic tool for hereditary spherocytosis.
Flow cytometric analysis of eosin-5'-maleimide-labeled red blood cells has been proposed as a new method of identifying hereditary spherocytosis (HS). The aim of the present study was to analyze sensitivity and specificity of this method. Red blood cells from patients with HS (n = 58) revealed significantly lower mean channel fluorescence values than red blood cells from normal subjects (n = 110), unaffected HS family members (n = 8), and patients with other anemias (n = 44). Taking a mean channel fluorescence of 400.0 units as the threshold value identified by logistic regression, sensitivity and specificity of the test for HS were 96.6 and 99.1%, respectively. Flow cytometric analysis is a valuable screening test for the diagnosis of HS.
Stoya
Gudrun
G
Institute of Anatomy I, Friedrich Schiller University, Jena, Germany. gsto@mti.uni-jena.de
Gruhn
Bernd
B
Vogelsang
Heinz
H
Baumann
Eckehard
E
Linss
Werner
W
D000743:Anemia, Hemolytic / Q000175:diagnosis; D004801:Eosine Yellowish-(YS) / Q000031:analogs & derivatives*; D004912:Erythrocytes / Q000737:chemistry*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016015:Logistic Models; D012680:Sensitivity and Specificity; D013103:Spherocytosis, Hereditary / Q000175:diagnosis*
C033531:eosin maleimide; D004801:Eosine Yellowish-(YS)