Analysis of dencichine in Panax notoginseng by gas chromatography-mass spectrometry with ethyl chloroformate derivatization.
Dencichine (beta-N-oxalyl-l-alpha,beta-diaminopropionic acid) is a haemostatic agent present in well-known traditional Chinese medicinal herbs such as Panax notoginseng, as well as other Panax species. It is also a reported neurotoxic agent found in Lathyrus sativus (grass pea seed) and cycad seeds. A method was developed for quantitative determination of the non-protein amino acid, dencichine, in plant samples of P. notoginseng and the adventitious roots directly from the explants of P. notoginseng after derivatization with ethyl chloroformate (ECF) by gas chromatography-mass spectrometry (GC-MS). l-2-chlorophenylalanine was used as an internal standard. Calibration curves were linear (r(2)=0.9988, n=6) in the range of 10-800 microg/ml for dencichine. Limit of detection and quantification for dencichine were 0.5 microg/ml and 2 microg/ml, respectively. This rapid and specific method may be applied to the quantification of dencichine in complex medicinal plants and their products.
Xie
Guo-Xiang
GX
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China.
Qiu
Yun-Ping
YP
Qiu
Ming-Feng
MF
Gao
Xian-Fu
XF
Liu
Yu-Min
YM
Jia
Wei
W
D000596:Amino Acids / Q000032:analysis / Q000302:isolation & purification; D000599:Amino Acids, Diamino / Q000032:analysis*; D010134:Fenclonine / Q000032:analysis; D005563:Formic Acid Esters / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results
D000596:Amino Acids; D000599:Amino Acids, Diamino; D005563:Formic Acid Esters; D007202:Indicators and Reagents; C007658:ethyl chloroformate; C100126:oxalyldiaminopropionic acid; D010134:Fenclonine