Embryonic cortical neural stem cells migrate ventrally and persist as postnatal striatal stem cells.
Embryonic cortical neural stem cells apparently have a transient existence, as they do not persist in the adult cortex. We sought to determine the fate of embryonic cortical stem cells by following Emx1(IREScre); LacZ/EGFP double-transgenic murine cells from midgestation into adulthood. Lineage tracing in combination with direct cell labeling and time-lapse video microscopy demonstrated that Emx1-lineage embryonic cortical stem cells migrate ventrally into the striatal germinal zone (GZ) perinatally and intermingle with striatal stem cells. Upon integration into the striatal GZ, cortical stem cells down-regulate Emx1 and up-regulate Dlx2, which is a homeobox gene characteristic of the developing striatum and striatal neural stem cells. This demonstrates the existence of a novel dorsal-to-ventral migration of neural stem cells in the perinatal forebrain.
Willaime-Morawek
Sandrine
S
Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario M5S 1A8, Canada. swmorawek@gmail.com
Seaberg
Raewyn M
RM
Batista
Claudia
C
Labbé
Etienne
E
Attisano
Liliana
L
Gorski
Jessica A
JA
Jones
Kevin R
KR
Kam
Angela
A
Morshead
Cindi M
CM
van der Kooy
Derek
D
D000818:Animals; D001479:Basal Ganglia / Q000166:cytology; D019070:Cell Lineage; D002465:Cell Movement; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology; D004622:Embryo, Mammalian / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis; D018398:Homeodomain Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018715:Microscopy, Video; D009474:Neurons / Q000166:cytology*; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D000099260:Empty Spiracles Homeobox Proteins
D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins; D014157:Transcription Factors; D000099260:Empty Spiracles Homeobox Proteins; C088334:Distal-less homeobox proteins