title: Deregulation of tissue homeostasis in endometria from patients with polycystic ovarian syndrome with and without endometrial hyperplasia.
abstract: OBJECTIVE
  To study the proteins involved in endometrial homeostasis in PCOS women.
  
  
  METHODS
  Protein expression of Ki67, Bcl-2, Bax, Pro-Caspase-3 and Caspase-3 by immunohistochemistry and/or Western blot, and DNA fragmentation using in situ 3'-end labeling of apoptotic cells, was measured in 9 samples of normal endometrium (NE), 12 PCOS endometria without treatment (PCOSE), 7 endometria from PCOS women with endometrial hyperplasia (HPCOSE) and 9 endometria from patients with endometrial hyperplasia (HE).
  
  
  RESULTS
  Cell proliferation was higher in epithelium from PCOSE (P<0.05), HPCOSE and HE vs NE. A higher Bcl-2/Bax relative ratio in PCOSE and HPCOSE was observed, in absence of active Caspase-3 and scarce DNA fragmentation in the four groups of endometria studied.
  
  
  CONCLUSIONS
  As the apoptosis was scarce in all of the groups studied, endometrial homeostasis deregulation in PCOS could be a result of increased proliferation. Therefore, the onset of endometrial hyperplasia in PCOS endometrium could be linked to inadequate cell proliferation, and concomitantly to inadequate cell survival.
authors.affiliation: Institute of Maternal and Child Research, School of Medicine, P.O. Box 226-3, and Pathology Department, San Borja-Arriarán Clinical Hospital, University of Chile, Santiago, Chile.
authors.forename: A
authors.initials: A
authors.lastname: Villavicencio
authors.forename: K
authors.initials: K
authors.lastname: Bacallao
authors.forename: F
authors.initials: F
authors.lastname: Gabler
authors.forename: A
authors.initials: A
authors.lastname: Fuentes
authors.forename: J
authors.initials: J
authors.lastname: Albornoz
authors.forename: A
authors.initials: A
authors.lastname: Casals
authors.forename: M
authors.initials: M
authors.lastname: Vega
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D053148:Caspase 3 / Q000096:biosynthesis; D048708:Cell Growth Processes / Q000502:physiology; D053938:DNA Fragmentation; D004714:Endometrial Hyperplasia / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006706:Homeostasis; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D019394:Ki-67 Antigen / Q000096:biosynthesis; D011085:Polycystic Ovary Syndrome / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
chemical_list: C494826:BAX protein, human; D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D053148:Caspase 3