title: Evaluation of the efficacy provided by a Recombinant Canarypox-Vectored Equine West Nile Virus vaccine against an experimental West Nile Virus intrathecal challenge in horses.
abstract: Efficacy of the Recombitek Equine West Nile Virus (WNV) vaccine was evaluated against a WNV intrathecal challenge model that results in WNV-induced clinical disease. Ten vaccinated (twice at days 0 and 35) and 10 control horses were challenged 2 weeks after administration of the second vaccine with a virulent WNV by intrathecal administration. After the challenge, eight of 10 controls developed clinical signs of encephalomyelitis whereas one vaccinate exhibited muscle fasciculation only once. Nine controls and one vaccinate developed a fever. Histopathology revealed mild to moderate nonsuppurative encephalitis in eight controls and one vaccinate. None of the vaccinates and all of the controls developed WNV viremia after challenge. All vaccinated horses developed antibodies to WNV after vaccination. These and results of previous studies demonstrate efficacy of the Recombitek WNV vaccine against WNV-induced clinical disease and natural challenge with WNV-infected mosquitoes.
authors.affiliation: Merial Limited, 115 Transtech Drive, Athens, GA 30601, USA.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Siger
authors.forename: Richard
authors.initials: R
authors.lastname: Bowen
authors.forename: Kemal
authors.initials: K
authors.lastname: Karaca
authors.forename: Michael
authors.initials: M
authors.lastname: Murray
authors.forename: Shyla
authors.initials: S
authors.lastname: Jagannatha
authors.forename: Brandy
authors.initials: B
authors.lastname: Echols
authors.forename: Robert
authors.initials: R
authors.lastname: Nordgren
authors.forename: Jules M
authors.initials: JM
authors.lastname: Minke
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D028121:Canarypox virus / Q000276:immunology; D009033:Culicidae / Q000821:virology*; D005260:Female; D006734:Horse Diseases / Q000517:prevention & control*; D006736:Horses; D008297:Male; D011897:Random Allocation; D016896:Treatment Outcome; D014766:Viremia / Q000662:veterinary; D014901:West Nile Fever / Q000517:prevention & control / Q000662:veterinary*; D053060:West Nile Virus Vaccines / Q000276:immunology*; D014902:West Nile virus / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D000914:Antibodies, Viral; D053060:West Nile Virus Vaccines