A cost-effective modified micromethod for measuring urine iodine.
A modified micromethod for measuring urine iodine was successfully established and validated. The micromethod showed good correlation with the method used by several World Health Organization (WHO) collaborative laboratories (y = 0.9342x + 4.6213; r = 0.962; p = 0.01; n = 50). The micromethod also showed good agreement when compared to the reference WHO method. The sensitivity of the assay was 13.809 ug/L (n = 8) and mean recoveries were 114, 103 and 106% at concentrations of 30, 40 and 50 ug/L (n = 3) respectively. At iodine concentrations of 51 +/- 15.5, 108 +/- 32.4 and 149 +/- 38.6 ug/L, intra-assay coefficient of variations (CVs) were 13%, 7% and 5% respectively (n = 20), and inter-assay CVs were 10%, 15% and 7% respectively (n = 10). The assay showed good linearity plot (y = 1.0407x + 60.451; r = 0.993; n = 3).
Husniza
Hussain
H
Cardiovascular, Diabetes & Nutrition Research Centre, Institute for Medical Research (IMR), Kuala Lumpur, Malaysia.
Wan Nazaimoon
Wan Mohamud
WM
D003362:Cost-Benefit Analysis; D006801:Humans; D007455:Iodine / Q000652:urine*; D014944:World Health Organization
D007455:Iodine