title: Protective effect of Mediterranean fish oil extracts on heat-induced denaturation of albumin.
abstract: Three oily extracts, obtained by acetone extraction from the entrails of different varieties of Mediterranean fishes, such as mackerel (Scomber scombrus), sardine (Sardina pilchardus) and horse mackerel (Trachurus mediterraneus), were characterized to determine their unsaturated fatty acid content. In an in-vitro model, their inhibitory effect was then evaluated against protein aggregation and their protective efficacy against heat-induced albumin denaturation assessed. The fish oil extracts tested in this study presented a significant amount of unsaturated fatty acids; in particular the extract obtained from the entrails of horse mackerel proved to have higher concentrations of DHA (docosahexaenoic acid) and oleic acid compared with the other two oils. The in-vitro study revealed an interesting protective effect of the oil extracts (particularly the horse mackerel extract) against heat-induced denaturation of albumin.
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Catania, Catania, Italy. capuglia@unict.it
authors.forename: Carmelo
authors.initials: C
authors.lastname: Puglia
authors.forename: Natale Alfredo
authors.initials: NA
authors.lastname: Santagati
authors.forename: Francesco
authors.initials: F
authors.lastname: Bonina
authors.forename: Domenico
authors.initials: D
authors.lastname: Trombetta
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Cristani
authors.forename: Antonio
authors.initials: A
authors.lastname: Speciale
authors.forename: Antonella
authors.initials: A
authors.lastname: Saija
mesh_terms: D000818:Animals; D002417:Cattle; D004281:Docosahexaenoic Acids / Q000302:isolation & purification / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification / Q000494:pharmacology*; D005395:Fish Oils / Q000737:chemistry*; D006358:Hot Temperature; D009792:Oceans and Seas; D009829:Oleic Acids / Q000302:isolation & purification / Q000494:pharmacology; D010473:Perciformes; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D011489:Protein Denaturation; D012709:Serum Albumin / Q000737:chemistry*
chemical_list: D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D009829:Oleic Acids; D020011:Protective Agents; D012709:Serum Albumin; D004281:Docosahexaenoic Acids