title: [Relationship between single nucleotide polymorphisms of NRAMP1 gene and susceptibility to pulmonary tuberculosis in workers exposed to silica dusts].
abstract: OBJECTIVE
  To explore the relationship between polymorphisms of natural resistance-associated macrophage protein 1 (NRAMP1) gene and genetic susceptibility of pulmonary tuberculosis (PTB) in workers exposed to silica dusts.
  
  
  METHODS
  A 1:2 case control study of 61 male workers with PTB (50 silicosis patients and 11 unsilicosis workers) as the case group and 122 male PTB-free workers (100 silicosis patients and 22 unsilicosis workers) as the control group was conducted with the frequency matched for age of +/- 5 years, the job, the silica exposure, and the condition of cigarette smoking and alcohol drinking. The polymerase chain reaction-restrained fragment length polymorphism technique (PCR-RFLP) was used to detect the single nucleotide polymorphisms (SNPs) of NRAMP1 INT4 and D543N.
  
  
  RESULTS
  There was a 2.73 times (95% CI: 1.32 approximately 5.64) increased risk of silicosis for individuals with C allele of NRAMP1 INT4 compared with individuals carrying homozygote (G/G), while SNPs of NRAMP1 D543N was not associated with PTB (P > 0.05).
  
  
  CONCLUSIONS
  The G > C mutation of intron 4 of NRAMP1 gene might be a susceptible factor of silica for the workers exposed to PTB.
authors.affiliation: Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China.
authors.forename: Ya-bin
authors.initials: YB
authors.lastname: Qu
authors.forename: Yun-xia
authors.initials: YX
authors.lastname: Tang
authors.forename: Zhong-bin
authors.initials: ZB
authors.lastname: Zhang
authors.forename: Ren
authors.initials: R
authors.lastname: Zhu
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Shou-yong
authors.initials: SY
authors.lastname: Gu
authors.forename: Guo-liang
authors.initials: GL
authors.lastname: Lu
authors.forename: Zhao-lin
authors.initials: ZL
authors.lastname: Xia
mesh_terms: D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D027682:Cation Transport Proteins / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012829:Silicosis / Q000150:complications*; D014397:Tuberculosis, Pulmonary / Q000150:complications / Q000235:genetics*; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D027682:Cation Transport Proteins; D000099173:Natural Resistance-associated Macrophage Protein 1