title: Isolation and characterization of dihydromonacolin-MV from Monascus purpureus for antioxidant properties.
abstract: The methanolic extract of Monascus purpureus cultivated by solid-state fermentation on rice showed strong 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and better yield as compared to other polarity based extracted fractions. It was selected for further purification of the antioxidant. The activity-guided repeated fractionation of methanolic extract on a silica gel column chromatography yielded a compound that exhibited strong antioxidant activity. Based on the spectroscopic analysis by UV, IR, 1H NMR, 13C NMR, 2D-HSQCT NMR, and MS, the antioxidant isolated was elucidated as a derivative of dihydromonacolin-K, where the ester group is 2-methyl propionate, designated as dihydromonacolin-MV. The DPPH radical was significantly scavenged by the dihydromonacolin-MV (IC50 20+/-1 microg ml-1). The dihydromonacolin-MV showed strong inhibition of lipid peroxidation in a liposome model with an IC50 value of 5.71+/-0.38 microg ml-1 and superoxide radical scavenging activity with an IC50 value of 163.97+/-2.68 microg ml-1.
authors.affiliation: Department of Food Microbiology, Central Food Technological Research Institute, Mysore, 570020, India.
authors.forename: Mohan A
authors.initials: MA
authors.lastname: Dhale
authors.forename: S
authors.initials: S
authors.lastname: Divakar
authors.forename: S Umesh
authors.initials: SU
authors.lastname: Kumar
authors.forename: G
authors.initials: G
authors.lastname: Vijayalakshmi
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002845:Chromatography; D005285:Fermentation; D007202:Indicators and Reagents / Q000494:pharmacology; D015227:Lipid Peroxidation; D013058:Mass Spectrometry; D015394:Molecular Structure; D032741:Monascus / Q000378:metabolism*; D009281:Naphthalenes / Q000302:isolation & purification* / Q000494:pharmacology*; D012275:Oryza / Q000378:metabolism; D010851:Picrates / Q000494:pharmacology; D058428:Silica Gel; D012822:Silicon Dioxide; D013057:Spectrum Analysis
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D007202:Indicators and Reagents; D009281:Naphthalenes; D010851:Picrates; D058428:Silica Gel; D012822:Silicon Dioxide; C004931:1,1-diphenyl-2-picrylhydrazyl