title: Mass spectrometric detection of tissue proteins in plasma.
abstract: It has long been thought that blood plasma could serve as a window into the state of one's organs in health and disease because tissue-derived proteins represent a significant fraction of the plasma proteome. Although substantial technical progress has been made toward the goal of comprehensively analyzing the blood plasma proteome, the basic assumption that proteins derived from a variety of tissues could indeed be detectable in plasma using current proteomics technologies has not been rigorously tested. Here we provide evidence that such tissue-derived proteins are both present and detectable in plasma via direct mass spectrometric analysis of captured glycopeptides and thus provide a conceptual basis for plasma protein biomarker discovery and analysis.
authors.affiliation: Institute for Systems Biology, Seattle, Washington 98103, USA. hzhang23@jhmi.edu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Alvin Y
authors.initials: AY
authors.lastname: Liu
authors.forename: Paul
authors.initials: P
authors.lastname: Loriaux
authors.forename: Bernd
authors.initials: B
authors.lastname: Wollscheid
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Julian D
authors.initials: JD
authors.lastname: Watts
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
mesh_terms: D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D006020:Glycopeptides / Q000097:blood* / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000097:blood* / Q000737:chemistry
chemical_list: D015703:Antigens, CD; D006020:Glycopeptides; D009363:Neoplasm Proteins