title: [Differential regulation of P2X3 mRNA expression in the rat trigeminal ganglion after experimental tooth movement].
abstract: OBJECTIVE
  To investigate the regulation of P2X3 mRNA expression in the trigeminal ganglion sensory neurons after the nociceptive stimulation by orthodontic tooth movement force.
  
  
  METHODS
  Male Sprague-Dawley rats weighing 200-250 g were used. The mimic tooth movement appliance was used in the rats of the experiment group. The rats were sacrificed after 4 h, 12 h, 1 d, 2 d, 3 d, 5 d and 7 d after experiment. The expression of P2X3 mRNA was detected by in situ hybridization with an oligonucleotide probe.
  
  
  RESULTS
  After force was given to the teeth of rats, the number of P2X3 mRNA positive neurons increased on the first day and reached the peak on the third day; then, the level of P2X3 mRNA expression began to decrease, and after seven days, it returned to the level of the control group.
  
  
  CONCLUSIONS
  The results suggest that the P2X3 mRNA expression is transiently upregulated and anterogradely transported in trigeminal primary sensory neurons after orthodontic tooth movement and that P2X3 receptor may play a role in the pathomechanism of nociception in primary sensory neurons during orthodontic treatment.
authors.affiliation: Department of Orthodontics, West China Stomatology Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Lai
authors.forename: Yang-xi
authors.initials: YX
authors.lastname: Chen
mesh_terms: D000818:Animals; D008297:Male; D023421:Models, Animal; D009967:Orthodontic Appliances; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis* / Q000235:genetics; D058477:Receptors, Purinergic P2X3; D014087:Tooth Movement Techniques; D012668:Trigeminal Ganglion / Q000378:metabolism*
chemical_list: C546410:P2rx3 protein, rat; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3