Proteomics characterization of protein adsorption onto hemodialysis membranes.
Protein-adsorptive properties are a key feature of membranes used for hemodialysis treatment. Protein adsorption is vital to the biocompatibility of a membrane material and influences membrane's performance. The object of the present study is to investigate membrane biocompatibility by correlating the adsorbed proteome repertoire with structural feature of the membrane surfaces. Minidialyzers of identical structural characteristics composed of either cellulose diacetate or ethylenevinyl alcohol materials were employed to develop an ex vivo apparatus to investigate protein adsorption. Adsorbed proteins were eluted by a strong chaotropic buffer condition and investigated by 2-DE coupled to both MALDI-TOF mass spectrometry (MS) mass fingerprinting and fragmentation analysis on a nanoLC-MS/MS hybrid instrument. Membrane surface characterization included evaluation of roughness (atomic force microscopy), elemental chemical composition (X-ray-photoelectron-spectroscopy), and hydrophilicity (pulsed nuclear magnetic resonance). The present study identifies a number of different proteins as common or characteristic of filter material interaction, showing that proteomic techniques are a promising approach for the investigation of proteins surface-adsorbed onto hemodialysis membrane. Proteomic analysis enables the characterization of protein layers of unknown composition.
Bonomini
Mario
M
Istituto di Clinica Nefrologica, Dipartimento di Medicina, Università G. D'Annunzio di Chieti-Pescara, Centro Studi sull'Invecchiamento (Ce.S.I.), Chieti, Italy. m.bonomini@nephro.unich.it
Pavone
Barbara
B
Sirolli
Vittorio
V
Del Buono
Francesca
F
Di Cesare
Moreno
M
Del Boccio
Piero
P
Amoroso
Luigi
L
Di Ilio
Carmine
C
Sacchetta
Paolo
P
Federici
Giorgio
G
Urbani
Andrea
A
D000327:Adsorption; D001798:Blood Proteins / Q000032:analysis*; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D011145:Polyvinyls / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D006435:Renal Dialysis / Q000295:instrumentation*; D013499:Surface Properties
D001798:Blood Proteins; D008567:Membranes, Artificial; D011145:Polyvinyls; D020543:Proteome; C020320:ethylene-vinyl alcohol copolymer; D002482:Cellulose; C026170:cellulose diacetate