A novel and simple ORAC methodology based on the interaction of Pyrogallol Red with peroxyl radicals.
Oxygen radicals absorbance capacities (ORAC) indexes are frequently employed to characterize the radical trapping capacity of pure compounds and their complex mixtures. A drawback of ORAC values obtained using phycoerythrin, fluorescein (FL) or c-phycocyanin as targets, makes it possible to conclude that for very reactive compounds they are much more related to stoichiometric factors than to the reactivity of the tested compound. In the present paper, we propose a simple methodology, based on the bleaching of Pyrogallol Red (PGR) absorbance that provides ORAC indexes that are almost exclusively determined by the reactivity of the tested compounds. This difference is due to the high reactivity of PGR and the high concentrations of this compound employed in the experiments.
López-Alarcón
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University of Santiago, Faculty of Chemistry and Biology, Santiago, Chile.
Lissi
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D005609:Free Radicals / Q000737:chemistry; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry*; D010545:Peroxides / Q000737:chemistry*; D011748:Pyrogallol / Q000031:analogs & derivatives* / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000737:chemistry*; D013997:Time Factors
D005609:Free Radicals; D010545:Peroxides; D017382:Reactive Oxygen Species; D011748:Pyrogallol; C049375:perhydroxyl radical; C058731:pyrogallol sulfonphthalein; D010100:Oxygen