title: [A study on the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein].
abstract: OBJECTIVE
  To study the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein.
  
  
  METHODS
  Four specific siRNAs were obtained by transcription in vitro. After transfection with the siRNAs into HepG2.2.15 cells for 3 days, the inhibitive effects of La protein were analyzed by Western blot; the content changes of HBsAg, HBeAg and HBV-DNA were detected by ECL and RT-PCR.
  
  
  RESULTS
  In comparison to normal cells, La protein was less in the cells. There was less La protein in the cells trans-infected with siRNAs. HBsAg, the HBeAg and HBV-DNA secreted by the cells transfected with siRNA were also less than that in the normal cells.
  
  
  CONCLUSIONS
  There is a correlation between La protein and HBV mRNA and the expression of HBV protein.
authors.affiliation: Clinical Laboratory, Oriental Hospital, Shanghai 201200, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Jin-Hui
authors.initials: JH
authors.lastname: Sun
authors.forename: Hong-Lian
authors.initials: HL
authors.lastname: Geng
authors.forename: Lie-Ying
authors.initials: LY
authors.lastname: Fan
authors.forename: Gao-Lin
authors.initials: GL
authors.lastname: Liu
authors.forename: Long-Yi
authors.initials: LY
authors.lastname: Tan
mesh_terms: D001324:Autoantigens / Q000378:metabolism*; D045744:Cell Line, Tumor; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006515:Hepatitis B virus / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering; D012367:RNA, Viral; D012261:Ribonucleoproteins / Q000378:metabolism*; D000097585:SS-B Antigen
chemical_list: D001324:Autoantigens; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012367:RNA, Viral; D012261:Ribonucleoproteins; D000097585:SS-B Antigen