Highly sensitive gold nanoparticles biosensor chips modified with a self-assembled bilayer for detection of Con A.
In this paper, an improved method for detection of Concanavalin A (Con A) with label-free optical biosensors is reported. 1-Dodecanethiol (DDT) was self-assembled onto gold nanoparticles which were deposited on glass slides, and then glycolipid molecules were inserted into dodecanethiol by physical interactions only. The recognition between Con A and carbohydrate was observed by UV-vis spectrophotometry. The absorption spectrum shifted when Con A was bound to the sugar residues of glycolipids immobilized onto nanogold slides, while almost no spectrum change was observed when another nonspecific protein molecule met the nanogold slides. The self-assembled bilayer on nanogold substrates had very high sensitivity for Con A, the minimum detection concentration of Con A can be down to 0.1 nM. In addition to the ultra sensitivity for investigating carbohydrate-lectin interaction, the self-assembled bilayer structure, is expected to replace many receptors which require time-consuming organic syntheses for the fixation to the transducer. The simplicity and sensitivity of this biosensor architecture once again show the prospect of nanogold application in biosensor.
Guo
Caixin
C
Key Laboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing 100080, PR China.
Boullanger
Paul
P
Jiang
Long
L
Liu
Tao
T
D015374:Biosensing Techniques / Q000295:instrumentation*; D003208:Concanavalin A / Q000032:analysis*; D006017:Glycolipids; D017979:Gold Colloid; D053758:Nanoparticles; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds
D006017:Glycolipids; D017979:Gold Colloid; D013438:Sulfhydryl Compounds; D003208:Concanavalin A; C013976:dodecylmercaptan