[Changes in kinetic properties of superoxide dismutase and glutathione peroxidase in the liver and erythrocytes from rats during deficiency of protein and additional dietary administration Cu, Zn, Mn and Se].
The kinetic parameters of liver and erythrocyte superoxide dismutase (SOD) and glutatione peroxidase (GP) have been investigated in rats kept under conditions of low protein diet (8%) and during addition of organic compounds containing Cu, Zn, Mn, Se. There were differences in kinetic parameters of hepatic and erythrocyte enzymes. After 2 and 4 weeks of maintenance of rats at a semi-synthetic diet there was an increase of Km value of liver and erythrocyte GP for tert-butyl hydroperoxide. After two weeks there was a decrease in Km of SOD for NADH-O2*- generating system. The low protein diet and consumption of Cu, Zn, Mn, Se caused a decrease of Km value of liver and erythrocytes GP and development of oxidative stress in liver as a result dietary administration Cu and Mn.
Vasil'ev
A V
AV
Ivakhnenko
V I
VI
Mal'tsev
G Iu
GIu
D000818:Animals; D003300:Copper / Q000008:administration & dosage; D019587:Dietary Supplements; D004912:Erythrocytes / Q000201:enzymology*; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007700:Kinetics; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology*; D008297:Male; D008345:Manganese / Q000008:administration & dosage; D009942:Organometallic Compounds / Q000008:administration & dosage; D011488:Protein Deficiency / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014131:Trace Elements / Q000008:administration & dosage; D015032:Zinc / Q000008:administration & dosage
D009942:Organometallic Compounds; D014131:Trace Elements; D008345:Manganese; D003300:Copper; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium; D015032:Zinc