Recruitment of RNA polymerase to Class II CRP-dependent promoters is improved by a second upstream-bound CRP molecule.
Genetics and biochemistry have been exploited to investigate transcription activation by the Escherichia coli CRP (cAMP receptor protein) factor at promoters with a DNA site for CRP near position -41 and the effects of a second upstream-bound CRP molecule. We show that the upstream-bound CRP contributes to transcription activation by improving the recruitment of RNA polymerase.
Miroslavova
N S
NS
School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK.
Mitchell
J E
JE
Tebbutt
J
J
Busby
S J W
SJ
D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D007700:Kinetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics*; D011953:Receptors, Cyclic AMP / Q000235:genetics / Q000378:metabolism
D004269:DNA, Bacterial; D011953:Receptors, Cyclic AMP; D012319:RNA Polymerase II