title: Targeting vector construction by yeast artificial chromosome modification.
abstract: Mouse yeast artificial chromosomes (YACs) are useful platforms for manipulation of targeting vector design and construction, particularly in circumstances where polymerase chain reaction-mediated amplification of targeting arms proves fruitless or the cloned DNA is inherently unstable. This chapter describes a simple procedure where YACs can be modified to produce targeting vectors of various sizes.
authors.affiliation: Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Murray
mesh_terms: D000818:Animals; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015723:Gene Library; D018390:Gene Targeting; D005822:Genetic Vectors; D051379:Mice; D008810:Mice, Inbred C57BL; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013234:Stem Cells / Q000502:physiology