Production system for biodegradable polyester polyhydroxybutyrate by Corynebacterium glutamicum.
A biosynthetic pathway for poly(3-hydroxybutyrate) [P(3HB)] production by Corynebacterium glutamicum was developed by introducing the phbCAB operon derived from Ralstonia eutropha. P(3HB) synthase activity was detected in this recombinant C. glutamicum carrying a cell surface protein gene promoter. Intracellular P(3HB) was microscopically observed as inclusion granules and its content was calculated to be 22.5% (w/w) with a number average molecular weight of 2.1x10(5) and a polydispersity of 1.63.
Jo
Sung-Jin
SJ
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Sapporo-shi, Hokkaido, Japan.
Maeda
Michihisa
M
Ooi
Toshihiko
T
Taguchi
Seiichi
S
D048230:Corynebacterium glutamicum / Q000166:cytology / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D020118:Cupriavidus necator / Q000201:enzymology / Q000235:genetics*; D006885:Hydroxybutyrates / Q000378:metabolism*; D002479:Inclusion Bodies / Q000235:genetics / Q000378:metabolism*; D009876:Operon / Q000235:genetics; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates