title: Hepatopancreatic nuclease of black tiger shrimp Penaeus monodon unlikely to be involved in viral triggered apoptosis.
abstract: Nucleases are phosphodiesterases that hydrolyze DNA and/or RNA. In a search for shrimp nucleases involved in apoptosis, we discovered a nuclease from hepatopancreatic cDNA of the black tiger shrimp Penaeus monodon. The full-length nuclease gene was amplified and revealed to contain 1668bp corresponding to 381 deduced amino acid residues in the mature enzyme. Sequence analysis indicated 83% nucleic acid identity and 89% amino acid identity to a nuclease from the Kuruma shrimp Penaeus japonicus (also called Marsupenaeus japonicus). Comparative analysis of sequences, conserved motifs and phylogenetic trees indicated that P. monodon nuclease (PMN) belonged to the family of DNA/RNA non-specific endonucleases (DRNSN). RT-PCR analysis using primers specific for PMN mRNA with seven different shrimp tissues revealed that expression in normal shrimp was restricted to the hepatopancreas. Semiquantitative RT-PCR analysis of PMN using hepatopancreatic mRNA from normal shrimp and from shrimp challenged with white spot syndrome virus (WSSV) indicated significant up-regulation of PMN in the hepatopancreas (P<0.05) at the early stage of viral infection but a return to baseline levels as gross signs of disease developed. At the same time, expression was always confined to the hepatopancreas and never seen in other tissues, including those reported to be prime targets for WSSV and subject to increased levels of apoptosis after infection. The results suggested that PMN is probably a digestive enzyme that is unlikely to be involved in hallmark DNA digestion associated with apoptosis.
authors.affiliation: Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. sctwf@mahidol.ac.th
authors.forename: Sudkhate
authors.initials: S
authors.lastname: Molthathong
authors.forename: Jiraporn
authors.initials: J
authors.lastname: Rojtinnakorn
authors.forename: Saengchan
authors.initials: S
authors.lastname: Senapin
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Flegel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D001665:Binding Sites; D004720:Endonucleases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D043143:Hepatopancreas / Q000201:enzymology; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000201:enzymology* / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013997:Time Factors; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D004720:Endonucleases