title: Blockade of adenosine A2A receptors prevents protein phosphorylation in the striatum induced by cortical stimulation.
abstract: Previous studies have shown that cortical stimulation selectively activates extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation and immediate early gene expression in striatal GABAergic enkephalinergic neurons. In the present study, we demonstrate that blockade of adenosine A2A receptors with caffeine or a selective A2A receptor antagonist counteracts the striatal activation of cAMP-protein kinase A cascade (phosphorylation of the Ser845 residue of the glutamate receptor 1 subunit of the AMPA receptor) and mitogen-activated protein kinase (ERK1/2 phosphorylation) induced by the in vivo stimulation of corticostriatal afferents. The results indicate that A2A receptors strongly modulate the efficacy of glutamatergic synapses on striatal enkephalinergic neurons.
authors.affiliation: Behavioral Neuroscience Branch, National Institute on Drug Abuse, Intramural Research Program, National Institutes of Health, Department of Health and Human Services, Baltimore, Maryland 21224, USA.
authors.forename: César
authors.initials: C
authors.lastname: Quiroz
authors.forename: Catarina
authors.initials: C
authors.lastname: Gomes
authors.forename: Arlene C
authors.initials: AC
authors.lastname: Pak
authors.forename: Joaquim A
authors.initials: JA
authors.lastname: Ribeiro
authors.forename: Steven R
authors.initials: SR
authors.lastname: Goldberg
authors.forename: Bruce T
authors.initials: BT
authors.lastname: Hope
authors.forename: Sergi
authors.initials: S
authors.lastname: Ferré
mesh_terms: D058917:Adenosine A2 Receptor Antagonists; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004558:Electric Stimulation / Q000379:methods; D008297:Male; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D043705:Receptor, Adenosine A2A / Q000502:physiology; D014970:Xanthines / Q000494:pharmacology
chemical_list: D058917:Adenosine A2 Receptor Antagonists; C113602:MSX 3 compound; D043705:Receptor, Adenosine A2A; D014970:Xanthines