[Construction of protein fingerprint signature in serum and its significance for early diagnosis with chronic allograft nephropathy].
OBJECTIVE
To construct protein fingerprints in serum and to early diagnose chronic allograft nephropathy (CAN) using surface enhanced laser ionization/desorption-time of flight-mass spectrometry (SELDI-TOF-MS).


METHODS
Serum protein spectra were detected by SELDI-TOF-MS and weak cation exchange (CM10), which were from long-term survival patients with well-functioning kidney allograft (LS) (n = 24) and CAN (n = 15), and then biomarkers were screened through the analysis of database with Biomarker Wizard and Biomarker Pattern softwares.


RESULTS
78 protein peaks of interest were generated in each sample, 18 protein spectra statistically show difference between LS and CAN (P < 0.05), 6 of which (m/z 2476.0, 3078.7, 3190.5, 4076.5, 4506.0, 6178.4) statistically show significant difference (P < 0.01), a single biomarker (m/z 3078.7) differentiated LTS and CAN (P = 0.0001), it was upregulated in the former and downregulated in the latter, its diagnostic sensitivity and specificity were 87.5% and 81.8% respectively. Its cost-effective value is 0.307.


CONCLUSIONS
Establishment of protein fingerprint signature in serum by SELDI-TOF-MS is clinically predictive of CAN.
Zhou
Shao-tang
ST
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Yan
Ji-huan
JH
Yang
Cheng
C
Zeng
Ning
N
Wu
Ke
K
Chen
Zhong-hua
ZH
D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000378:metabolism*; D002908:Chronic Disease; D042241:Early Diagnosis; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007674:Kidney Diseases / Q000097:blood* / Q000175:diagnosis*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D010449:Peptide Mapping; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017741:Survivors
D001798:Blood Proteins