Targeting vector construction by yeast artificial chromosome modification.
Mouse yeast artificial chromosomes (YACs) are useful platforms for manipulation of targeting vector design and construction, particularly in circumstances where polymerase chain reaction-mediated amplification of targeting arms proves fruitless or the cloned DNA is inherently unstable. This chapter describes a simple procedure where YACs can be modified to produce targeting vectors of various sizes.
Murray
Peter J
PJ
Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA.
D000818:Animals; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015723:Gene Library; D018390:Gene Targeting; D005822:Genetic Vectors; D051379:Mice; D008810:Mice, Inbred C57BL; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013234:Stem Cells / Q000502:physiology