CE to monitor endotoxins by protein complexation.
A new CE method for fast and efficient analysis of bacterial endotoxins (lipopolysaccharides) is described. It is based on the strong interaction between proteins and endotoxins. The UV absorption of the protein component in the complex is used for the detection. The electrophoretic mobility of the complex hemoglobin/endotoxin can be employed for qualitative analysis of the endotoxin. For instance, the structural differences between "smooth" and "rough" lipopolysaccharides from Salmonella minnesota (wild-type), Salmonella minnesota R595 and Shigella sonnei R562H are reflected in the electrophoretic mobilities of their hemoglobin complex.
Kilár
Anikó
A
Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Pécs, Hungary.
Kocsis
Béla
B
Kustos
Ildikó
I
Kilár
Ferenc
F
Hjertén
Stellan
S
D001427:Bacterial Toxins / Q000032:analysis* / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006454:Hemoglobins / Q000737:chemistry; D008070:Lipopolysaccharides / Q000032:analysis / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012475:Salmonella / Q000737:chemistry*; D012764:Shigella sonnei / Q000737:chemistry*
D001427:Bacterial Toxins; D006454:Hemoglobins; D008070:Lipopolysaccharides; D011506:Proteins