Effect of dehydroepiandrosterone (DHEA) treatment on oxidative energy metabolism in rat liver and brain mitochondria. A dose-response study.
OBJECTIVE
Effects of treatment with dehydroepiandrosterone (DHEA) on oxidative energy metabolism in rat liver and brain mitochondria were examined.


METHODS
Young adult rats were administered DHEA (0.1, 0.2, 1.0 or 2.0 mg/kg body weight) by subcutaneous route for 7 consecutive days.


RESULTS
DHEA treatment resulted in general, in stimulation of state 3 respiration rates without having any uncoupling effect on ADP/O ratios. The stimulation of state 3 respiration rate for a given substrate was dose dependent in a tissue-specific manner. Parallel increases in the contents of cytochromes aa(3) and b were also noted. DHEA treatment stimulated the glutamate dehydrogenase (GDH) and succinate DCIP reductase (SDR) activities. Under the treatment conditions, mitochondrial ATPase activity was also stimulated.


CONCLUSIONS
Treatment with DHEA significantly stimulated oxidative energy metabolism in liver and brain mitochondria.
Patel
Minal A
MA
Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat 390 002, India.
Katyare
Surendra S
SS
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D003580:Cytochromes / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D003687:Dehydroepiandrosterone / Q000008:administration & dosage / Q000494:pharmacology*; D018698:Glutamic Acid / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D051381:Rats; D013379:Substrate Specificity / Q000187:drug effects; D019802:Succinic Acid / Q000378:metabolism; D013772:Tetramethylphenylenediamine / Q000378:metabolism
D003580:Cytochromes; D008293:Malates; D018698:Glutamic Acid; D003687:Dehydroepiandrosterone; C030298:malic acid; D019289:Pyruvic Acid; D019802:Succinic Acid; D010088:Oxidoreductases; D000251:Adenosine Triphosphatases; D013772:Tetramethylphenylenediamine; D001205:Ascorbic Acid