title: [Peritoneal fluid leptin concentration and endocrine hormone in patients with polycystic ovarian syndrome].
abstract: OBJECTIVE
  To determine whether the infertile patients with polycystic ovarian syndrome (PCOS) is related to dysregulation of peritoneal fluid and serum leptin concentration, and to investigate the relationship between the leptin and some endocrine hormones in PCOS.
  
  
  METHODS
  Twenty subjects with PCOS and 20 control women were included in the study. Peritoneal fluid and serum concentration of leptin, insulin, insulin-antibody, testosterone (T), estrogen (E(2)), and progestogen (P) were measured by radioimmunoassay (RIA).
  
  
  RESULTS
  Peritoneal fluid concentrations of leptin, insulin, T and insulin-antibody in PCOS patients were significantly higher than those of the control group (P<0.05). There was no statistically significant difference in peritoneal fluid E(2) and P between PCOS and the control group (P>0.05). The serum concentrations of leptin and T in PCOS were significantly higher than those of the control group (P<0.05), but the levels of insulin, E(2), P and insulin-antibody were not significantly different between the 2 groups (P>0.05). With the BMI> or =23 kg/m(2) subgroup in PCOS patients, the peritoneal fluid and serum concentrations of leptin, insulin and T were significantly higher than those of BMI 23 kg/m(2) subgroup (P<0.01). There was no significant difference in E(2)and insulin-antibody between the 2 subgroups (P>0.05). Pearson correlation analysis indicated that peritoneal fluid and serum leptin levels were positively correlated with BMI, insulin, T and insulin-antibody, but negatively correlated with E(2), with no significant correlation with P. Multiple stepwise regression analysis indicated that the factors that influenced the peritoneal fluid and serum leptin levels were BMI, insulin, T and E(2) ordinally.
  
  
  CONCLUSIONS
  Peritoneal fluid and serum leptin concentration and insulin,T, Ins-antibody level are abnormal in PCOS patients. Leptin may play an important role in the pathogenesis of PCOS. BMI is the main factor to correlate with leptin.
authors.affiliation: Department of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Song-shu
authors.initials: SS
authors.lastname: Xiao
authors.forename: Min
authors.initials: M
authors.lastname: Xue
authors.forename: Xin-liang
authors.initials: XL
authors.lastname: Deng
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Wan
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000378:metabolism*; D001323:Autoantibodies / Q000096:biosynthesis; D004967:Estrogens / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007328:Insulin / Q000096:biosynthesis* / Q000276:immunology; D020738:Leptin / Q000096:biosynthesis*; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis; D013739:Testosterone / Q000096:biosynthesis*
chemical_list: D001323:Autoantibodies; D004967:Estrogens; D007328:Insulin; D020738:Leptin; D013739:Testosterone; D011374:Progesterone