title: Potentiation of inhibitory amino acid receptors-mediated responses by lanthanum in rat sacral dorsal commissural neurons.
abstract: Lanthanum is one of rare earth cations with extremely active chemical property and has been reported to influence neuronal transmitter systems. To date, little attention has been directed towards the sacral dorsal commissural nucleus (SDCN), which serves as a relay of sensory information from the pelvic viscera in the spinal cord. Therefore, the effect of lanthanum on the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) and glycine (Gly) responses in neurons acutely dissociated from the rat SDCN was investigated using the nystatin-perforated patch-recording configuration under voltage-clamp conditions. At a holding potential of -40 mV, La(3+) reversibly potentiated GABA (3 microM)-activated currents (I(GABA)) in a concentration-dependent manner over the concentration range of 10 microM to 30 mM, with the EC(50) value of 67.3+/-16.4 microM. Similarly, La(3+) reversibly potentiated glycine (10 microM)-activated currents (I(Gly)) in a concentration-dependent manner over the concentration range of 1 microM to 1 mM, with the EC(50) value of 52.3+/-10.9 microM. The effects of La(3+) on I(GABA) and I(Gly) were voltage-independent. Moreover, both of the potentiations were not use-dependent and were overcome by increasing the concentration of agonist. Our results indicate that La(3+) potentiates the inhibitory amino acid receptors-mediated responses in SDCN, which may reduce the transmission of the pelvic visceral information. The information provided by this work may help to elucidate the mechanisms and effects of lanthanum on brain functions.
authors.affiliation: Institute of Functional Brain Disorders, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Chong-Wang
authors.initials: CW
authors.lastname: Chang
authors.forename: Bo
authors.initials: B
authors.lastname: Qu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Hong
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Gao
mesh_terms: D000465:Algorithms; D000818:Animals; D004594:Electrophysiology; D066298:In Vitro Techniques; D007811:Lanthanum / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D009761:Nystatin / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D017459:Receptors, Amino Acid / Q000187:drug effects*; D011963:Receptors, GABA-A / Q000187:drug effects; D018009:Receptors, Glycine / Q000187:drug effects; D013116:Spinal Cord / Q000166:cytology* / Q000187:drug effects
chemical_list: D017459:Receptors, Amino Acid; D011963:Receptors, GABA-A; D018009:Receptors, Glycine; D009761:Nystatin; D007811:Lanthanum