[Experimental research of Shuanghuang Shengbai granule on hematopoietic cells of leucopenia model mice].
OBJECTIVE
To investigate the leukogenic function of Shuanghuang Shengbai (SHSB) granule and the related mechanisms.


METHODS
Mouse leukopenic models were induced by radiation. Mice were divided into normal control group, model control group, positive control group-Li kejun tablet group and three different dose (high, middle, low-dose) groups of SHSB granule. The peripheral hemogram, thymus index (TI), spleen index (SI), bone marrow nucleated cell (BMNC) and colony forming unit-spleen (CFU-S) were evaluated. The proliferation of bone marrow cells was determined. The in vitro cultured colony forming unit granulocyte macrophage (CFU-GM) was estimated. The index of CD34+ cell in BMNC were determined by flow cytometry. The ultra-micro structure of bone marrow were observed by electromicroscope.


RESULTS
(1)SHSB rranule could increase the WBC of model mice; (2)SHSB granule could increase BMNC and promote the proliferation of bone marrow cell; (3)SHSB granule could increase CFU-S, CFU-GM and CD34+ cell index in BMNC of model mice significantly; (4)SHSB Granule could also protect the bone marrow hemotopoietic microenvironment from the harm of radiation; (5)SHSB granule could increase the SI of model mice, indicating the enhancement of immunological function.


CONCLUSIONS
SHSB granule has apparent leukogenic function. The mechanism may be related to enhancing the proliferation of hematopoietic cells and protecting the bone marrow hemotopoietic microenvironment.
Shen
Yun-hui
YH
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine.
Chen
Chang-xun
CX
Xu
Zhen-ye
ZY
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D007958:Leukocyte Count; D007970:Leukopenia / Q000276:immunology / Q000473:pathology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010946:Plants, Medicinal / Q000737:chemistry
D018952:Antigens, CD34; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal