title: Population genetic structure of the dengue mosquito Aedes aegypti in Venezuela.
abstract: The mosquito Aedes aegypti is the main vector of dengue in Venezuela. The genetic structure of this vector was investigated in 24 samples collected from eight geographic regions separated by up to 1160 km. We examined the distribution of a 359-basepair region of the NADH dehydrogenase subunit 4 mitochondrial gene among 1144 Ae. aegypti from eight collections. This gene was amplified by the polymerase chain reaction and tested for variation using single strand conformation polymorphism analysis. Seven haplotypes were detected throughout Venezuela and these were sorted into two clades. Significant differentiation was detected among collections and these were genetically isolated by distance.
authors.affiliation: Centro de Investigaciones Biomédicas, Universidad de Carabobo Callejón Cecilio Acosta, Avda. Las Delicias, Maracay 2101, Aragua, Venezuela. flormhq@cantv.net
authors.forename: Flor
authors.initials: F
authors.lastname: Herrera
authors.forename: Ludmel
authors.initials: L
authors.lastname: Urdaneta
authors.forename: José
authors.initials: J
authors.lastname: Rivero
authors.forename: Normig
authors.initials: N
authors.lastname: Zoghbi
authors.forename: Johanny
authors.initials: J
authors.lastname: Ruiz
authors.forename: Gabriela
authors.initials: G
authors.lastname: Carrasquel
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Martínez
authors.forename: Martha
authors.initials: M
authors.lastname: Pernalete
authors.forename: Patricia
authors.initials: P
authors.lastname: Villegas
authors.forename: Ana
authors.initials: A
authors.lastname: Montoya
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Rubio-Palis
authors.forename: Elina
authors.initials: E
authors.lastname: Rojas
mesh_terms: D000330:Aedes / Q000201:enzymology / Q000235:genetics*; D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D003715:Dengue / Q000635:transmission; D014644:Genetic Variation / Q000235:genetics*; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D007303:Insect Vectors / Q000201:enzymology / Q000235:genetics*; D009245:NADH Dehydrogenase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D014687:Venezuela
chemical_list: D004272:DNA, Mitochondrial; C098483:NADH dehydrogenase subunit 4; D009245:NADH Dehydrogenase