title: Substrate flexibility of a 2,6-dideoxyglycosyltransferase.
abstract: We report the first 2,6-dideoxysugar-O-glycosyltransferase with substrate flexibility at the 2 position, confirm the function of a putative NDP-hexose 2,3-dehydratase in the jadomycin B biosynthetic gene cluster and deduce the substrate flexibility of downstream enzymes in l-digitoxose assembly, enabling reprogramming of biosynthetic gene clusters to modify sugar substituents.
authors.affiliation: College of Pharmacy, Dalhousie University, 5968 College St., Halifax, Nova Scotia, Canada. david.jakeman@dal.ca
authors.forename: David L
authors.initials: DL
authors.lastname: Jakeman
authors.forename: Charles N
authors.initials: CN
authors.lastname: Borissow
authors.forename: Cathy L
authors.initials: CL
authors.lastname: Graham
authors.forename: Shannon C
authors.initials: SC
authors.lastname: Timmons
authors.forename: Taryn R
authors.initials: TR
authors.lastname: Reid
authors.forename: Ray T
authors.initials: RT
authors.lastname: Syvitski
mesh_terms: D016695:Glycosyltransferases / Q000737:chemistry*; D006601:Hexoses / Q000737:chemistry; D007546:Isoquinolines / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008968:Molecular Conformation; D009005:Monosaccharides / Q000737:chemistry; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D006601:Hexoses; C519266:ILEVS1080; D007546:Isoquinolines; D009005:Monosaccharides; C081750:jadomycin B; C020615:digitoxose; D016695:Glycosyltransferases