Engineering of GAL1 promoter-driven expression system with artificial transcription factors.
We isolated and characterized artificial transcription factors (ATFs) that functionally activate GAL1 promoter in yeast. These ATFs transformed the yeast galactose-dependent GAL1 promoter system into a galactose-independent one. The ATFs were identified by screening a combinatorial library of zinc finger-containing transcription factors for components that activated the transcription of a reporter gene under the control of a truncated GAL1 promoter from which the GAL4p-binding sites were deleted. We also showed that these ATFs activate transcription from GAL1 promoter by binding directly to specific sequence elements in the promoter.
Park
Kyung-Soon
KS
College of Medicine, Pochon CHA University, 606-16 Yeoksam1-dong, Gangnam-gu, Seoul 135-081, South Korea. pks@chahealthsystems.co.kr
Kim
Jin-Soo
JS
D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D005690:Galactose / Q000494:pharmacology / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers
D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005690:Galactose