[Effects of Siwu Tang on protein expression of HFCL].
OBJECTIVE
To investigate the effects of Siwu Tang on HFCL proliferation and protein expression of bone marrow stromal cells and to discuss molecular mechanism of enriching blood functions of Siwu Tang.


METHODS
MTT, flow cytometry and proteomics were used respectively to measure the effect of Siwu Tang on HFCL proliferation, cell cycle and proteinexpression.


RESULTS
Siwu Tang can promote HFCL proliferation, increase proliferative index, up-regulate 12 kinds of proteins and down-regulate 5 kinds of proteins of HFCL.


CONCLUSIONS
Through promoting HFCL proliferation and acting on multipleprotein targets, the blood enriching function of Siwu Tang was exerted indirectly.
Guo
Ping
P
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing, China.
Wang
Sheng-qi
SQ
D029971:Angelica sinensis / Q000737:chemistry; D001854:Bone Marrow Cells / Q000378:metabolism; D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006801:Humans; D030004:Ligusticum / Q000737:chemistry; D008516:Medicine, Chinese Traditional; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D050737:Proto-Oncogene Proteins c-crk / Q000378:metabolism; D032267:Rehmannia / Q000737:chemistry; D017154:Stromal Cells / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D011506:Proteins; D050737:Proto-Oncogene Proteins c-crk; D051028:bcl-2-Associated X Protein; C403258:si-wu-tang; D016222:Fibroblast Growth Factor 2