Attomole microarray detection of microRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions.
Multiple microRNAs (miRNAs) are detected in a microarray format using a novel approach that combines a surface enzyme reaction with nanoparticle-amplified SPR imaging (SPRI). The surface reaction of poly(A) polymerase creates poly(A) tails on miRNAs hybridized onto locked nucleic acid (LNA) microarrays. DNA-modified nanoparticles are then adsorbed onto the poly(A) tails and detected with SPRI. This ultrasensitive nanoparticle-amplified SPRI methodology can be used for miRNA profiling at attomole levels.
Fang
Shiping
S
Department of Chemistry, University of California-Irvine, Irvine, CA 92697, USA.
Lee
Hye Jin
HJ
Wark
Alastair W
AW
Corn
Robert M
RM
D000249:Adenosine Monophosphate / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis*; D053758:Nanoparticles; D020411:Oligonucleotide Array Sequence Analysis; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D035683:MicroRNAs; D000249:Adenosine Monophosphate