[Determination of papaverine in Qiangli Pipalu by HPLC].
OBJECTIVE
To establish a method for the determination of the papaverine content in Qiangli Pibalu by HPLC.


METHODS
A C18 column with a solvent system of acetonitrile-0.02 mol x L(-1) sodium dihydrogen phosphate (0.2% triethylamine, phosphoric acid, at pH 3) (25:75) and UV detection 240 nm were used. The flow rate was 1.0 mL x min(-1). The column temperature was maintained at 40 degrees C.


RESULTS
There was a good linear relationship between the absorption value and the concentration in the range of 0.020 2-0.100 5 microg for papaverine. The average recovery rates were 99.1% (RSD 2.3%).


CONCLUSIONS
The method is simple, accurate and can be used to determine the contents in Qiangli Pibalu.
Qin
Wen-Jie
WJ
College of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
Ma
Kai
K
Gao
Han
H
Wang
Gang-Li
GL
Lin
Rui-Chao
RC
D000996:Antitussive Agents / Q000737:chemistry / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D031983:Eriobotrya / Q000737:chemistry; D005100:Expectorants / Q000737:chemistry / Q000494:pharmacology; D010207:Papaver / Q000737:chemistry; D010208:Papaverine / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results
D000996:Antitussive Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005100:Expectorants; D010208:Papaverine