Thyroid hormones induce sumoylation of the cold shock domain-containing protein PIPPin in developing rat brain and in cultured neurons.
We previously identified a cold shock domain (CSD)-containing protein (PIPPin), expressed at high level in brain cells. PIPPin has the potential to undergo different posttranslational modifications and might be a good candidate to regulate the synthesis of specific proteins in response to extracellular stimuli. Here we report the effects of T(3) on PIPPin expression in developing rat brain. We found that a significant difference among euthyroid and hypothyroid newborn rats concerns sumoylation of nuclear PIPPin, which is abolished by hypothyroidism. Moreover, T(3) dependence of PIPPin sumoylation has been confirmed in cortical neurons purified from brain cortices and cultured in a chemically defined medium (Maat medium), with or without T(3). We also report that about one half of unmodified as well as all the sumoylated form of PIPPin could be extracted from nuclei with HCl, together with histones. Moreover, this HCl-soluble fraction remains in the nucleus even after treatment with 0.6 M KCl, thus suggesting strong interaction of PIPPin with nuclear structures and perhaps chromatin.
Bono
Epifania
E
Dipartimento di Biologia Cellulare e dello Sviluppo Alberto Monroy, University of Palermo, 90127 Palermo, Italy.
Compagno
Vincenza
V
Proia
Patrizia
P
Raimondi
Lavinia
L
Schiera
Gabriella
G
Favaloro
Vincenzo
V
Campo
Valerio
V
Donatelli
Maria
M
Di Liegro
Italia
I
D000818:Animals; D000831:Animals, Newborn; D013956:Antithyroid Agents / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D005260:Female; D007037:Hypothyroidism / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011441:Propylthiouracil / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D025842:SUMO-1 Protein / Q000378:metabolism*; D014284:Triiodothyronine / Q000494:pharmacology*
D013956:Antithyroid Agents; C097845:Csdc2 protein, rat; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; D025842:SUMO-1 Protein; D014284:Triiodothyronine; D011441:Propylthiouracil