title: Diagnosis of loxoscelism in a child confirmed with an enzyme-linked immunosorbent assay and noninvasive tissue sampling.
abstract: BACKGROUND
  Confirmation of mild bites caused by Loxosceles reclusa with swab testing has not been previously documented, to our knowledge.
  
  
  METHODS
  We report a case using an enzyme-linked immunosorbent assay (ELISA) test.
  
  
  RESULTS
  A lesion lacking necrosis or other specific signs of loxoscelism was confirmed by identification of the Loxosceles venom and further confirmed by identification of a spider found in the patient's bed.
  
  
  CONCLUSIONS
  This is a pilot single-case report for this enzyme-linked immunosorbent assay test.
  
  
  CONCLUSIONS
  A sensitive and specific enzyme-linked immunosorbent assay designed to detect Loxosceles venom, using a specimen obtained by swabbing the lesion, can aid in diagnosis of loxoscelism.
authors.affiliation: Dermatology Center, Rolla, MO 65401-4600, USA. wvs@ece.umr.edu
authors.forename: William V
authors.initials: WV
authors.lastname: Stoecker
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Green
authors.forename: Hernan F
authors.initials: HF
authors.lastname: Gomez
mesh_terms: D000818:Animals; D001512:Bedding and Linens; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D013048:Specimen Handling / Q000379:methods*; D001098:Spider Bites / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D013111:Spider Venoms / Q000032:analysis*; D013112:Spiders
chemical_list: D013111:Spider Venoms; C055161:loxosceles venom; D010727:Phosphoric Diester Hydrolases