A Toll receptor in shrimp.
Outbreaks of infectious diseases have resulted in high mortality and huge economic losses in penaeid shrimp culture. Interest in understanding shrimp immunity has increased because of its importance in disease control. Here we report cDNA cloning of a Toll receptor from the white shrimp Litopenaeus vannamei. L. vannamei Toll (lToll) is 926 residues, with a putative signal peptide of 19 residues. The protein contains distinct structural/functional motifs of the Toll like receptor (TLR) family, including an extracellular domain containing 16 leucine-rich repeats (LRRs) flanked by cysteine-rich motifs and a cytoplasmic Toll/interleukin-1 receptor (TIR) domain. The lToll TIR domain showed high similarity to Apis mellifer Toll and Drosophila melanogaster Toll, with 59.9% and 54.3% identity, respectively. Reverse-transcription polymerase chain reaction (RT-PCR) analysis showed that lToll was expressed in hemocyte, gill, heart, brain, stomach, intestine, pyloric caecum, muscle, nerve and spermary, with a lower expression level in eyestalk and hepatopancreas. Identification of lToll will help to elucidate the Toll pathway in shrimp innate immunity.
Yang
Li-Shi
LS
State Key Laboratory for Biocontrol, School of Life Sciences, Zhongshan University, 135 Xingang Road West, Guangzhou 510275, PR China.
Yin
Zhi-Xin
ZX
Liao
Ji-Xiang
JX
Huang
Xian-De
XD
Guo
Chang-Jun
CJ
Weng
Shao-Ping
SP
Chan
Siu-Ming
SM
Yu
Xiao-Qiang
XQ
He
Jian-Guo
JG
D020816:Amino Acid Motifs / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D004331:Drosophila melanogaster; D005786:Gene Expression Regulation / Q000276:immunology; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology; D010802:Phylogeny; D017434:Protein Structure, Tertiary / Q000235:genetics; D051193:Toll-Like Receptors / Q000235:genetics* / Q000276:immunology
D051193:Toll-Like Receptors