Extensive cross-reactive neutralizing antibody response in Indian patients with limited genetic diversity of HIV-1.
Genome sequence analysis of HIV-1 subtype C viruses from India shows monophyletic lineage and relatively limited genetic diversity. To understand its immunological implication, cross-reactivity of neutralizing antibody response was investigated. In primary screening, neutralizing antibody response to single heterologous primary HIV-1 subtype C isolate was assessed in plasma samples from 235 HIV-1 infected, anti-retroviral treatment naive individuals from Pune, India. Plasma samples that showed > or =90% neutralization and two randomly selected plasma samples that showed 50-60% neutralization were tested against a panel of primary HIV-1 subtype C isolates obtained from epidemiologically unlinked individuals from India. The neutralizing antibody response showed extensive cross-neutralization, suggesting presence of shared neutralization determinants among circulating HIV-1 subtype C viruses in India.
Lakhashe
Samir K
SK
National AIDS Research Institute, G-73, MIDC, Bhosari, Pune 411 026, India.
Kulkarni
Smita S
SS
Thakar
Madhuri R
MR
Ghate
Manisha V
MV
Paranjape
Ramesh S
RS
D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology*; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000378:metabolism; D015658:HIV Infections / Q000453:epidemiology / Q000276:immunology* / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D009500:Neutralization Tests
D000914:Antibodies, Viral; D015699:HIV Envelope Protein gp120