Aromatase immunoreactivity in the bluehead wrasse brain, Thalassoma bifasciatum: immunolocalization and co-regionalization with arginine vasotocin and tyrosine hydroxylase.
Sex steroid hormones regulate various neural functions that control vertebrate sociosexual behavior. A number of sex steroids can be synthesized de novo in the brain, including estrogens by the enzyme aromatase. Aromatase, the neuropeptides arginine vasotocin/vasopressin, and the monoamine neurotransmitter dopamine have all been implicated in the control of male sexual and aggressive behavior in a variety of vertebrates. This study examined the expression of brain aromatase in the bluehead wrasse (Thalassoma bifasciatum), a teleost fish that exhibits socially controlled behavioral and gonadal sex change. We used immunocytochemistry (ICC) to characterize distributions of aromatase-immunoreactive (ir) cells, and to examine their relationship with AVT-ir neurons and tyrosine hydroxylase-ir (TH-ir) neurons in key sensory and integrative areas of the brain of this species. Aromatase-ir appeared to be in glial cell populations, and was found in the dorsal and ventral telencephalon, the preoptic area of the hypothalamus, and the lateral recess of the third ventricle, among other brain areas. Aromatase-ir fibers are closely associated with AVT-ir neurons throughout the preoptic area, indicating the potential for functional interactions. Aromatase-ir cell bodies and fibers were also co-regionalized with TH-ir neurons, suggesting possible interaction between the dopaminergic system and neural estrogen production. The presence of aromatase in brain regions important in the regulation of sexual and aggressive behavior suggests that local estrogen synthesis could regulate sex change through effects on signaling systems that subserve reproductive behavior and function.
Marsh
K Erica
KE
North Carolina State University, Department of Zoology and W.M. Keck Center for Behavioral Biology, Raleigh, NC 27606, USA.
Creutz
Lela M
LM
Hawkins
M Beth
MB
Godwin
John
J
D000374:Aggression / Q000502:physiology; D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D001931:Brain Mapping; D004298:Dopamine / Q000096:biosynthesis*; D004967:Estrogens / Q000096:biosynthesis*; D005260:Female; D006066:Gonads / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010473:Perciformes / Q000033:anatomy & histology / Q000378:metabolism*; D012098:Reproduction / Q000502:physiology; D012733:Sex Differentiation / Q000502:physiology; D012726:Sexual Behavior, Animal / Q000502:physiology; D013045:Species Specificity; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014668:Vasotocin / Q000378:metabolism*
D004967:Estrogens; D001141:Aromatase; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine; D014668:Vasotocin