[Quality assurance of quantitative PCR with real-time reverse transcription polymerase chain reaction method].
The real-time reverse transcription polymerase chain reaction (RT-PCR) method is the most sensitive method for the detection of mRNA and DNA virus. The use of this real-time quantitative PCR(RQ-PCR) has been utilized increasingly to monitor gene expression of many types on clinical diagnosis, and has become standard for the detection and quantification of RNA targets. The employment of the techniques for RQ-PCR offers the advantages of high sensitivity and reproducibility. A number of RQ-PCR have been described that commercial PCR kits are available for quantitative analysis of a limited number of clinically important virus only. Quality assurance is necessary to be assessment the overall process and procedures of quantitative PCR as well as the other clinical testing. Therefore, it should be examined to be used with based on appropriately quality control.
Funato
Tadao
T
Department of Laboratory Science, School of Health Science, Faculty of Medicine, Kyoto University, Kyoto 606-8507.
Takeda
Mayu
M
D004267:DNA Viruses / Q000235:genetics; D005821:Genetic Techniques; D011785:Quality Assurance, Health Care; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000592:standards*; D012680:Sensitivity and Specificity
D012333:RNA, Messenger