Bisphenol A induces transforming growth factor-beta3 mRNA in the preoptic area: a cDNA expression array and Northern blot study.
To gain better understanding of the effects of bisphenol A (BPA) in the adult brain, a cDNA expression array was used to screen possible candidates for BPA-inducible genes in the medial preoptic area (MPOA). Adult ovariectomized rats were given a subcutaneous injection of 10 mg BPA or sesame oil alone as a control. Twenty-four hours after the injection, the MPOAs were dissected and total RNAs were extracted. When expression levels of cDNAs derived from pooled samples were compared to controls, the expression levels of some genes in BPA-injected rats appeared to be different from those in sesame oil-injected rats. Among the candidate genes, we focused on an increase in the expression of transforming growth factor (TGF)-beta3 mRNA. To quantify the change in TGF-beta3 mRNA by BPA, we examined the effects of 10 mg BPA (n=6), 1 microg 17beta-estradiol (n=6), or oil injection (n=6) on the expression of TGF-beta3 mRNA in the MPOA of ovariectomized rats by Northern blot. The TGF-beta3 mRNA level in the MPOA of BPA-injected rats was significantly increased compared to the level in oil-injected rats (p<0.05). Injection of 1 microg 17beta-estradiol did not have any significant effect. The results suggest that, in the adult female rat, BPA acts on the MPOA by altering the expression of the TGF-beta3 gene in a manner distinct from that of estrogen.
Fukushima
Atsushi
A
Department of Neuroendocrinology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.
Funabashi
Toshiya
T
Kawaguchi
Maiko
M
Mitsushima
Dai
D
Kimura
Fukuko
F
D000818:Animals; D001559:Benzhydryl Compounds; D015152:Blotting, Northern / Q000379:methods; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010052:Ovariectomy; D010636:Phenols / Q000494:pharmacology*; D011301:Preoptic Area / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D053782:Transforming Growth Factor beta3 / Q000235:genetics / Q000378:metabolism*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D012333:RNA, Messenger; D053782:Transforming Growth Factor beta3; C006780:bisphenol A; D000098826:Bisphenol A Compounds