title: [Expression of G-6-PD mRNA in children with G-6-PD deficiency].
abstract: OBJECTIVE
  To detect the level of glucose-6-phosphate dehydrogenase (G-6-PD) mRNA expression in children with G-6-PD deficiency and their lineal family members in order to explore possible mechanisms of the disease at the transcriptional level.
  
  
  METHODS
  RNA was extracted from peripheral blood of 41 children with G-6-PD deficiency and of their lineal family members (29 father lineages and 40 mother lineages). cDNA was then harvested using the reverse transcription method. G-6-PD mRNA expression was detected by quantitative real-time PCR (QRT-PCR). The detection results of G-6-PD mRNA expression in three groups (Patient, Father lineage and Mother lineage) were compared using Statistical Software SPSS 10.0 system.
  
  
  RESULTS
  The mean G-6-PD mRNA value in the Patient, Father lineage and Mother lineage groups were 0.57 +/- 0.19, 0.74 +/- 0.21 and 0.67 +/- 0.21 respectively. The G-6-PD mRNA value in the Patient group was significantly lower than both Father lineage (t = -3.18, P < 0.01) and Mother lineage groups (t = -2.54, P < 0.05).
  
  
  CONCLUSIONS
  The expression of G-6-PD mRNA decreased in children with G-6-PD deficiency, suggesting that the pathogenesis of this disorder relates to the varying levels of gene transcription.
authors.affiliation: Department of Hematology and Oncology, Children's Hospital of Shenzhen, Shenzhen, Guangdong 518026, China. Licg6336@sina.com
authors.forename: Chang-Gang
authors.initials: CG
authors.lastname: Li
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Chen
authors.forename: Yun-Sheng
authors.initials: YS
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong-Song
authors.initials: HS
authors.lastname: Shi
authors.forename: Wei-Ling
authors.initials: WL
authors.lastname: Zhao
authors.forename: Cheng-Rong
authors.initials: CR
authors.lastname: Li
mesh_terms: D002648:Child; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000235:genetics*; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D005954:Glucosephosphate Dehydrogenase