IQCJ-SCHIP1, a novel fusion transcript encoding a calmodulin-binding IQ motif protein.
The existence of transcripts that span two adjacent, independent genes is considered rare in the human genome. This study characterizes a novel human fusion gene named IQCJ-SCHIP1. IQCJ-SCHIP1 is the longest isoform of a complex transcriptional unit that bridges two separate genes that encode distinct proteins, IQCJ, a novel IQ motif containing protein and SCHIP1, a schwannomin interacting protein that has been previously shown to interact with the Neurofibromatosis type 2 (NF2) protein. IQCJ-SCHIP1 is located on the chromosome 3q25 and comprises a 1692-bp transcript encompassing 11 exons spanning 828kb of the genomic DNA. We show that IQCJ-SCHIP1 mRNA is highly expressed in the brain. Protein encoded by the IQCJ-SCHIP1 gene was localized to cytoplasm and actin-rich regions and in differentiated PC12 cells was also seen in neurite extensions.
Kwaśnicka-Crawford
Dorota A
DA
Department of Genetics and Genomic Biology, The Hospital for Sick Children, Toronto, Ont., Canada. dakc@yorku.ca
Carson
Andrew R
AR
Scherer
Stephen W
SW
D020816:Amino Acid Motifs; D002148:Calmodulin-Binding Proteins / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*
D002148:Calmodulin-Binding Proteins; C515556:IQCJ-SCHIP1 fusion protein, human; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors