title: Obestatin is not elevated or correlated with insulin in children with Prader-Willi syndrome.
abstract: BACKGROUND
  Obestatin is a peptide hormone derived from the proteolytic cleavage of ghrelin preprohormone. In Prader-Willi syndrome (PWS), the levels of total ghrelin (TG) and acylated ghrelin (AG) are increased, and these hormones are regulated by insulin.
  
  
  OBJECTIVE
  Our objective was to analyze the changes in the obestatin levels after glucose loading and to characterize the correlations of obestatin with TG, AG, and insulin.
  
  
  METHODS
  Plasma obestatin, TG, AG, and insulin levels were measured in PWS children (n = 15) and controls (n = 18) during an oral glucose tolerance test.
  
  
  METHODS
  All subjects were admitted to the Samsung Medical Center.
  
  
  METHODS
  An oral glucose tolerance test was performed after an overnight fast.
  
  
  METHODS
  The plasma levels of obestatin, TG, AG, and serum insulin were measured at 0, 30, 60, 90, and 120 min after glucose challenge, and areas under the curves (AUCs) were calculated.
  
  
  RESULTS
  No significant difference in AUC of the plasma obestatin was found between the PWS children and normal obese controls (P = 0.885), although AUC of AG (P = 0.002) and TG (P = 0.003) were increased in the PWS children. Moreover, There was a negative correlation between the AUC of AG and AUC of insulin both in PWS (r = -0.432; P = 0.049) and in controls (r = -0.507; P = 0.016). However, AUC of obestatin was not significantly correlated with AUC of insulin (in PWS, r = 0.168 and P = 0.275; in controls, r = -0.331 and P = 0.09).
  
  
  CONCLUSIONS
  Our results indicate that plasma obestatin is not elevated in PWS children and is not regulated by insulin both in PWS children and in obese controls.
authors.affiliation: Department of Orthopedic Sports Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 135-710 Seoul, Korea.
authors.forename: Won Hah
authors.initials: WH
authors.lastname: Park
authors.forename: Yoo Joung
authors.initials: YJ
authors.lastname: Oh
authors.forename: Gae Young
authors.initials: GY
authors.lastname: Kim
authors.forename: Sang Eun
authors.initials: SE
authors.lastname: Kim
authors.forename: Kyung-Hoon
authors.initials: KH
authors.lastname: Paik
authors.forename: Sun Ju
authors.initials: SJ
authors.lastname: Han
authors.forename: An Hee
authors.initials: AH
authors.lastname: Kim
authors.forename: Su Hyun
authors.initials: SH
authors.lastname: Chu
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Kwon
authors.forename: Seon Woo
authors.initials: SW
authors.lastname: Kim
authors.forename: Dong-Kyu
authors.initials: DK
authors.lastname: Jin
mesh_terms: D019540:Area Under Curve; D015992:Body Mass Index; D002648:Child; D005260:Female; D054439:Ghrelin; D006801:Humans; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance; D008297:Male; D036361:Peptide Hormones / Q000097:blood*; D011218:Prader-Willi Syndrome / Q000097:blood*; D014280:Triglycerides / Q000097:blood
chemical_list: D054439:Ghrelin; D007328:Insulin; D036361:Peptide Hormones; D014280:Triglycerides; C505799:obestatin, human