Expression of transforming growth factor-beta isoforms and their receptors in utero-vaginal junction of hen oviduct in presence or absence of resident sperm with reference to sperm storage.
Our goal was to determine whether transforming growth factor beta (TGFbeta) isoforms were involved in the process of sperm survivability in the sperm-storage tubules (SST) in the utero-vaginal junction (UVJ) of hen oviduct. The birds were artificially inseminated. The mRNA expressions of three types of TGFbeta isoforms (TGFbeta2, TGFbeta3, and TGFbeta4) and three types of receptors (TbetaR1, TbetaR2, and TbetaR3) were examined in the presence or in the absence of resident sperm in SST by semi-quantitative reverse transcriptase-PCR. The mRNA expression of TGFbetas and TbetaRs in sperm was also examined. Immunocytochemistry and western blot were performed for TbetaR2 to confirm its localization in UVJ. The sperm were observed at least 10 days after insemination by histology. The mRNA expressions of TGFbetas and TbetaRs were significantly increased in UVJ in the presence of resident sperm in SST. The mRNA expressions of TGFbetas and TbetaRs were also observed in sperm. Immunohistochemistry revealed that TbetaR2 were located in lymphocytes in UVJ and SST cells. The presence of TbetaR2 in UVJ was also confirmed by western blot. These results suggest that enhanced expressions of TGFbetas and TbetaRs in UVJ may protect sperm in SST, probably by suppressing anti-sperm immunoreactions.
Das
Shubash Chandra
SC
Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
Isobe
Naoki
N
Nishibori
Masahide
M
Yoshimura
Yukinori
Y
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002645:Chickens / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D010057:Oviducts / Q000737:chemistry*; D020033:Protein Isoforms / Q000032:analysis* / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013083:Sperm Transport; D013094:Spermatozoa / Q000378:metabolism; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000032:analysis* / Q000235:genetics; D014599:Uterus; D014621:Vagina
D020033:Protein Isoforms; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta