title: Assessment of the genetic component of hypertension.
abstract: BACKGROUND
  Although genetic epidemiologic studies have suggested that several genetic variants increase the risk for hypertension, the genes that underlie genetic susceptibility to this condition remain to be identified definitively. We have now performed a large-scale association study to identify gene polymorphisms for reliable assessment of the genetic component of hypertension.
  
  
  METHODS
  The study population comprised 4853 unrelated Japanese individuals, including 2818 subjects with hypertension (1677 men, 1141 women) and 2035 controls (1011 men, 1024 women). The genotypes for 150 polymorphisms of 128 candidate genes were determined with a method that combines the polymerase chain reaction and sequence-specific oligonucleotide probes with suspension array technology.
  
  
  RESULTS
  Multivariable logistic regression analysis with adjustment for age, sex, body mass index, and the prevalence of smoking revealed that four polymorphisms (1648G-->A in ITGA2, -30G-->A in GCK, A-->G in SAH, and 1117C-->A in PTGIS) were significantly (P < .01) associated with hypertension. A stepwise forward selection procedure demonstrated that ITGA2, GCK, and PTGIS genotypes significantly affected the prevalence of hypertension. Combined genotype analysis of these polymorphisms yielded a lowest odds ratio of 0.47 for the genotypes of AA or AG for ITGA2, GA or AA for GCK, and CC for PTGIS, which were present in 1.1% and 2.0% of hypertensive and control individuals, respectively.
  
  
  CONCLUSIONS
  These results suggest that the genotypes for ITGA2, GCK, and PTGIS may prove reliable for the assessment of the genetic component of hypertension. Determination of the combined genotypes for these genes may contribute to personalized prevention of this condition.
authors.affiliation: Department of Human Functional Genomics, Life Science Research Center, Mie University, Tsu, Japan. yamada@gene.mie-u.ac.jp
authors.forename: Yoshiji
authors.initials: Y
authors.lastname: Yamada
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Matsuo
authors.forename: Tomonori
authors.initials: T
authors.lastname: Segawa
authors.forename: Sachiro
authors.initials: S
authors.lastname: Watanabe
authors.forename: Kimihiko
authors.initials: K
authors.lastname: Kato
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hibino
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yokoi
authors.forename: Sahoko
authors.initials: S
authors.lastname: Ichihara
authors.forename: Norifumi
authors.initials: N
authors.lastname: Metoki
authors.forename: Hidemi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Kei
authors.initials: K
authors.lastname: Satoh
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Nozawa
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005260:Female; D005838:Genotype; D000081410:Germinal Center Kinases; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D039421:Integrin alpha2 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*
chemical_list: D000081410:Germinal Center Kinases; C056564:ITGA2B protein, human; D039421:Integrin alpha2; D003577:Cytochrome P-450 Enzyme System; D017346:Protein Serine-Threonine Kinases; C472456:PTGIS protein, human