A simple, sensitive, and rapid method for the determination of cotinine in urine by high-performance liquid chromatography with UV detection.
Cotinine levels in biological fluids are a reliable indicator of the presence of nicotine. In this paper, a simple and sensitive high-performance liquid chromatography (HPLC) procedure for the determination of cotinine in urine following liquid-liquid extraction with dichloromethane in an alkaline medium is described. Calibration curves show linearity over the 50 to 3000 ng/mL range with low intra- and interday variability as well as good selectivity and specificity. No solid-phase extraction is performed because the liquid dichloromethane extraction step yields excellent results. This method is a good alternative for routine analysis of urinary cotinine in laboratories where gas chromatography or HPLC-mass spectrometry is not available.
Rabbaa-Khabbaz
Lydia
L
Faculty of Pharmacy, Saint-Joseph University, Mathaf, Beirut, Lebanon. khabfam@yahoo.com
Abi Daoud
Rita
R
Karam-Sarkis
Dolla
D
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003367:Cotinine / Q000652:urine*; D006863:Hydrogen-Ion Concentration; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D003367:Cotinine