[ITS sequence of 9 Bupleurum species and its application in identification of Chaihu (Radix Buplurei)].
OBJECTIVE
The study the patterns of rDNA ITS sequence variation of 9 medicinal species of genus Bupleurum in China to find the DNA molecular markers for identification of these crude drugs.


METHODS
The ITS regions of these species were amplified by PCR and sequenced, and their sequences were analyzed by DNAssist Version 2.2.


RESULTS
Homologous alignment indicated that the consanguinity of the ITS sequences between genus Bupleurum and outgroups was lower than 75%, and that within genus Bupleurum was higher than 88%. For the same species, the consanguinity was higher than 99%.


CONCLUSIONS
The ITS sequences may serve as reliable molecular markers for identification of Radix Bupleuri.
Xie
Hui
H
School of Pharmacy, Fudan University, Shanghai 200032, China.
Chao
Zhi
Z
Huo
Ke-ke
KK
Wu
Bing-yi
BY
Pan
Sheng-li
SL
D029972:Bupleurum / Q000145:classification / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer