[Determination of diltiazem hydrochloride injection by high-performance liquid chromatography].
OBJECTIVE
To establish an high-performance liquid chromatography (HPLC) method for the determination of diltiazem hydrochloride injection.


METHODS
The assay was conducted on a NUCLEODUR C18 column with buffer solution (pH6.2)-acetonitrile-methanol (40:30:30) as the mobile phase and the detection wavelength was 240 nm.


RESULTS
Diltiazem hydrochloride and relevant substances could be separated under the condition. The linear concentration range of diltiazem hydrochloride was 4 - 200mg/L with the recovery of 100.22% (RSD = 0.37%).


CONCLUSIONS
The method may be used for the determination of diltiazem hydrochloride injection.
Tang
Ling
L
Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410013, China.
Zhang
Ting
T
Li
Xin-zhong
XZ
D002121:Calcium Channel Blockers / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004110:Diltiazem / Q000032:analysis*
D002121:Calcium Channel Blockers; D004110:Diltiazem