Soy protein containing isoflavones favorably influences macrophage lipoprotein metabolism but not the development of atherosclerosis in CETP transgenic mice.
The possibility that soy protein containing isoflavones influences the development of experimental atherosclerosis has been investigated in ovariectomized mice heterozygous for the human CETP transgene and for the LDL-receptor null allele (LDLr(+/-) CETP(+/-)). After ovariectomy at 8 wk of age they were fed a fat/cholesterol-rich diet for 19 wk and divided into three experimental groups: dietary unmodified soy protein containing isoflavones (mg/g of diet), either at low-dose (Iso Low, 0.272, n = 25), or at high-dose (Iso High, 0.535, n = 28); and the atherogenic diet containing an isoflavone-depleted alcohol-washed soy protein as a control group (n = 28). Aortic root lipid-stained lesion area (mean microm2 x 10(3) +/- SD) did not differ among Iso Low (12.3 +/- 9.9), Iso High (7.4 +/- 6.4), and controls (10.7 +/- 12.8). Autoantibody titers against plasma oxidized LDL did not differ among the experimental groups. Using the control mice as the reference value (100%), in vitro mouse peritoneal macrophage uptake of labeled acetylated LDL-cholesterol was lower in the Iso High (68%) than in the Iso Low (85%) group. The in vitro percent removal by exogenous HDL of labeled unesterified cholesterol from macrophages previously enriched with human [4- 14C]-cholesteryl oleate acetylated LDL was enhanced in the Iso High group (50%). In spite of these in vitro potentially antiatherogenic actions, soy protein containing isoflavones did not modify the average size of lipid-stained area in the aortic root.
Asakura
Leiko
L
Lipids Lab, University of São Paulo Medical School, São Paulo, Brazil.
Cazita
Patrícia M
PM
Harada
Lila M
LM
Nunes
Valéria S
VS
Berti
Jairo A
JA
Salerno
Alessandro G
AG
Ketelhuth
Daniel F J
DF
Gidlund
Magnus
M
Oliveira
Helena C F
HC
Quintão
Eder C R
EC
D000818:Animals; D050197:Atherosclerosis / Q000178:diet therapy / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D053480:Cholesterol Ester Transfer Proteins; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006579:Heterozygote; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood / Q000378:metabolism*; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010052:Ovariectomy; D011973:Receptors, LDL / Q000235:genetics; D030262:Soybean Proteins / Q000737:chemistry / Q000494:pharmacology*; D019076:Transgenes
C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D006023:Glycoproteins; D007529:Isoflavones; D008055:Lipids; D008074:Lipoproteins; D011973:Receptors, LDL; D030262:Soybean Proteins