title: Ly49B is expressed on multiple subpopulations of myeloid cells.
abstract: Using a novel mAb specific for mouse Ly49B, we report here that Ly49B, the last remaining member of the C57 Ly49 family to be characterized, is expressed at low levels on approximately 1.5% of spleen cells, none which are NK cells or T cells but which instead belong to several distinct subpopulations of myeloid cells defined by expression of CD11b and different levels of Gr1. Much larger proportions of bone marrow and peritoneal cells expressed Ly49B, all being CD11b+ and comprising multiple subpopulations defined by light scatter, F4/80, and Gr1 expression. Costaining for Ly49Q, also expressed on myeloid cells, revealed that Ly49B and Ly49Q were most strongly expressed on nonoverlapping subpopulations, Ly49Q(high) cells being mostly B220+CD4+ and/or CD8+, Ly49B+ cells lacking these markers. Myeloid populations that developed from bone marrow progenitors in vitro frequently coexpressed both Ly49B and Ly49Q, and Ly49B expression could be up-regulated by LPS, alpha-IFN, and gamma-IFN, often independently of Ly49Q. PCR analysis revealed that cultured NK cells and T cells contained Ly49B transcripts, and Ly49B expression could be detected on NK cells cultured in IL-12 plus IL-18, and on an immature NK cell line. Immunohistochemical studies showed that Ly49B expression in tissues overlapped with but was distinct from that of all other myeloid molecules examined, being particularly prominent in the lamina propria and dome of Peyer's patches, implicating an important role of Ly49B in gut immunobiology. In transfected cells, Ly49B was found to associate with SHP-1, SHP-2, and SHIP in a manner strongly regulated by intracellular phosphorylation events.
authors.affiliation: Institute of Cell and Molecular Biosciences, The Medical School, Newcastle NE2 4HH, United Kingdom.
authors.forename: Frances
authors.initials: F
authors.lastname: Gays
authors.forename: Jonathan G
authors.initials: JG
authors.lastname: Aust
authors.forename: Delyth M
authors.initials: DM
authors.lastname: Reid
authors.forename: Jane
authors.initials: J
authors.lastname: Falconer
authors.forename: Noriko
authors.initials: N
authors.lastname: Toyama-Sorimachi
authors.forename: Philip R
authors.initials: PR
authors.lastname: Taylor
authors.forename: Colin G
authors.initials: CG
authors.lastname: Brooks
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000032:analysis; D000950:Antigens, Ly / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D039481:CD11b Antigen / Q000032:analysis; D005260:Female; D000072181:Inositol Polyphosphate 5-Phosphatases; D016898:Interferon-alpha / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology; D037181:Lectins, C-Type / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D022423:Myeloid Cells / Q000187:drug effects / Q000276:immunology*; D055650:NK Cell Lectin-Like Receptor Subfamily A; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010766:Phosphorylation; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D019707:Receptors, Chemokine / Q000032:analysis; D011971:Receptors, Immunologic / Q000378:metabolism; D055630:Receptors, NK Cell Lectin-Like; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000950:Antigens, Ly; D039481:CD11b Antigen; C503133:Gr-1 protein, mouse; D016898:Interferon-alpha; D047908:Intracellular Signaling Peptides and Proteins; C527089:Klra17 protein, mouse; C527090:Klra2 protein, mouse; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D055650:NK Cell Lectin-Like Receptor Subfamily A; C501922:Sirpa protein, mouse; D012333:RNA, Messenger; D019707:Receptors, Chemokine; D011971:Receptors, Immunologic; D055630:Receptors, NK Cell Lectin-Like; C036763:monocyte-macrophage differentiation antigen; D007371:Interferon-gamma; D010744:Phosphoric Monoester Hydrolases; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D017027:Protein Tyrosine Phosphatases; C516725:Ptpn11 protein, mouse; C516727:Ptpn11 protein, rat; D000072181:Inositol Polyphosphate 5-Phosphatases