[Expressions of stromal cell-derived factor-1 and CXCR4 in endometrial carcinoma tissues and cell lines].
OBJECTIVE
To investigate the expressions of SDF-1 and CXCR4 in human endometrial carcinoma tissues.


METHODS
SDF-1 and CXCR4 protein expression levels were detected by immunohistochemical staining in 26 cases of atypic hyperplasia endometrium,simple hyperplasia endometrium and normal endometrium and 44 cases of endometrial carcinoma tissues.


RESULTS
Both SDF-1 mRNA and CXCR4 mRNA were expressed in Ishikawa, while no expression of SDF-1 mRNA was detected in HEC-1A. The rates of CXCR4 and SDF-1 protein expressions were 100% in glandular cells of human normal endometrial tissues, while 90.9% and 93.2% in glandular cells of endometrial carcinoma tissues. These immunoreactivities of SDF-1 and CXCR4 were significantly low in endometrial carcinoma tissues as compared with those in normal endometrium; and the intensities of immunohistochemical staining significantly decreasesd in immunoreactivity of SDF-1 and CXCR4 in low grade as compared with that in hight grade.


CONCLUSIONS
SDF-1 and CXCR4 were expressed differently in endometrial cell lines, human normal endometrial tissues,and endometrial carcinoma tissues. As compared with normal endometrium ,these were significantly low levels expression of SDF-1 and CXCR4 in endometrial carcinoma tissues.
Li
Xiao-ping
XP
Department of Gynecology, Peking University Peopleos Hospital, Beijing 100044, China.
Zhao
Dan
D
Gao
Min
M
Wang
Jian-liu
JL
Wei
Li-hui
LH
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D054377:Chemokine CXCL12 / Q000096:biosynthesis / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D019718:Receptors, CXCR4 / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D012333:RNA, Messenger; D019718:Receptors, CXCR4