title: [Estrogenicity of adzuki bean Phaseolus angularis wight. and its effect on proesterone receptor level in human breast cancer MCF-7 cells].
abstract: OBJECTIVE
  To explore the estrogenic activity of ethanol extract from adzuki bean Phaseolus angularis and its effect on progesterone receptor (PR) level of human breast cancer MCF-7 cells.
  
  
  METHODS
  The modified MCF-7 cell proliferation assay was used to evaluate the estrogenic activity of adzuki bean extract. And its effect on PR mRNA and protein were addressed using RT-PCR measurements and western blotting analysis respectively in which pure estrogen receptor antagonist ICI 182,780 was employed as the tool.
  
  
  RESULTS
  The estrogenic activity of adzuki bean extract at various concentrations (10-200 microg x mL(-1)), expressed as proliferative effect (PE) relative to that of solvent control, was examined. The results indicated that the extract of adzuki bean was able to induce MCF-7 cell growth with a maximum at 100 microg x mL(-1). The results of RT-PCR and western blotting showed that PR mRNA and protein could be significantly induced by adzuki bean extract in MCF-7 cells. Moreover, the specific estrogen receptor antagonist ICI 182,780 could block these reactions.
  
  
  CONCLUSIONS
  Ethanol extract of adzuki bean has the estrogen-like activities through the estrogen response pathway.
authors.affiliation: First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. xgzhang666@yahoo.com.cn
authors.forename: Xing-guo
authors.initials: XG
authors.lastname: Zhang
authors.forename: Qing-wei
authors.initials: QW
authors.lastname: Zhao
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D005260:Female; D000077267:Fulvestrant; D006801:Humans; D027805:Phaseolus / Q000737:chemistry; D048789:Phytoestrogens / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011980:Receptors, Progesterone / Q000096:biosynthesis* / Q000235:genetics; D012639:Seeds / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D004965:Estrogen Antagonists; D048789:Phytoestrogens; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D000077267:Fulvestrant; D004958:Estradiol