A remodeling system for the oligosaccharide chains on glycoproteins with microbial endo-beta-N-acetylglucosaminidases.
Endo-M, endo-beta-N-acetylglucosaminidase from Mucor hiemalis, transferred the complex type oligosaccharide of sialoglycopeptide to partially deglycosylated proteins (N-acetylglucosamine-attached proteins), which were prepared by excluding high-mannose type oligosaccharides from glycoproteins with Endo-H, endo-beta-N-acetylglucosaminidase from Streptomyces plicatus. This finding indicated that the high-mannose type oligosaccharides on glycoproteins can be changed to complex type ones by the transglycosylation activity of Endo-M. This is the first report of the establishment of a remodeling system for the different types of oligosaccharides on glycoproteins with microbial endo-beta-N-acetylglucosaminidases having different substrate specificities. Endo-M is a powerful tool for the in vitro synthesis of glycoproteins containing complex type oligosaccharides from glycoproteins produced by yeast.
Fujita
Kiyotaka
K
Graduate School of Biostudies, Kyoto University, Kitashirakawa, Kyoto 606-8502, Japan. kfujita@ms.kagoshima-u.ac.jp
Yamamoto
Kenji
K
D017127:Glycation End Products, Advanced / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D006031:Glycosylation; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000378:metabolism*; D008954:Models, Biological; D009090:Mucorales / Q000166:cytology / Q000201:enzymology*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry
D017127:Glycation End Products, Advanced; D006023:Glycoproteins; D009844:Oligosaccharides; D012259:Ribonuclease, Pancreatic; C504335:pancreatic ribonuclease B, bovine; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase