title: [Inhibitory effect of reinioside C on LOX-1 expression induced by ox-LDL].
abstract: OBJECTIVE
  To investigate the effect of reinioside C (RC) on the expression of lectin-like oxidized low density lipoprotein receptor (LOX)-1 mRNA and LOX-1 protein induced by oxidized low density lipoprotein (ox-LDL) in cultured human umbilical vein endothelial cells (HUVEC).
  
  
  METHODS
  HUVECs were cultured with ox-LDL (50 mg/L) for 24 h in the absence or presence of RC (1, 3, and 10 micromol/L). The expressions of LOX-1 mRNA and LOX-1 protein were examined by RT-PCR and Western-blot.
  
  
  RESULTS
  Incubation with ox-LDL (50 mg/L) significantly raised the expression of LOX-1 mRNA and LOX-1 protein,which was concentration-dependent.
  
  
  CONCLUSIONS
  RC can inhibit the increased expression of LOX-1 mRNA and LOX-1 protein induced by ox-LDL in HUVECs.
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: Bai
authors.forename: Guo-gang
authors.initials: GG
authors.lastname: Zhang
authors.forename: Rui-zheng
authors.initials: RZ
authors.lastname: Shi
authors.forename: Yuan-jian
authors.initials: YJ
authors.lastname: Li
authors.forename: Gui-shan
authors.initials: GS
authors.lastname: Tan
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
mesh_terms: D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D031801:Polygala / Q000737:chemistry*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011973:Receptors, LDL / Q000096:biosynthesis* / Q000235:genetics; D012503:Saponins / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; D011973:Receptors, LDL; D012503:Saponins; C099252:oxidized low density lipoprotein; C530274:reinioside C