Nucleotide diversity of Japanese isolates of infectious hematopoietic necrosis virus (IHNV) based on the glycoprotein gene.
Infectious hematopoietic necrosis virus (IHNV), a member of the genus Novirhabdovirus, causes a highly lethal disease of salmonid fish. In the present study, G gene nucleotide sequences of 9 Japanese IHNV isolates obtained from 1971 to 1996 were analyzed to evaluate the genetic diversity and compared with IHNV isolates from North America and Europe. A radial phylogenetic tree revealed 5 major clusters including 3 genogroups (U, M and L) for North American isolates and 1 genogroup for European isolates. Five Japanese isolates from 1971 to 1982 appeared in the cluster for genogroup U, while the remaining Japanese isolates from 1980 to 1996 formed a new genogroup, JRt (Japanese rainbow trout). Maximum nucleotide diversity among the Japanese isolates was 4.5%, which was greater than that within the North American isolates (3.6%), and the degree of nucleotide diversity within Japanese isolates was increased by inclusion of the genogroup JRt isolates. It was concluded that Japanese isolates shared a common source with the genogroup U of the North American isolates and that there were large divergences between Japanese isolates before and after the 1980s.
Nishizawa
T
T
Faculty of Fisheries Sciences, Hokkaido University, Hakodate, 041-8611, Japan.
Kinoshita
S
S
Kim
W S
WS
Higashi
S
S
Yoshimizu
M
M
D000818:Animals; D017931:DNA Primers / Q000737:chemistry; D005060:Europe; D005393:Fish Diseases / Q000821:virology; D014644:Genetic Variation / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D029142:Infectious hematopoietic necrosis virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009656:North America; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D018353:Rhabdoviridae Infections / Q000662:veterinary / Q000821:virology; D017858:Salmoniformes / Q000821:virology; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D006023:Glycoproteins