Binding properties of HABA-type azo derivatives to avidin and avidin-related protein 4.
The chicken genome encodes several biotin-binding proteins, including avidin and avidin-related protein 4 (AVR4). In addition to D-biotin, avidin binds an azo dye compound, 4-hydroxyazobenzene-2-carboxylic acid (HABA), but the HABA-binding properties of AVR4 are not yet known. Differential scanning calorimetry, UV/visible spectroscopy, and molecular modeling were used to analyze the binding of 15 azo molecules to avidin and AVR4. Significant differences are seen in azo compound preferences for the two proteins, emphasizing the importance of the loop between strands beta3 and beta4 for azo ligand recognition; information on these loops is provided by the high-resolution (1.5 A) X-ray structure for avidin reported here. These results may be valuable in designing improved tools for avidin-based life science and nanobiotechnology applications.
Repo
Susanna
S
Department of Biochemistry and Pharmacy, Abo Akademi University, Tykistökatu 6, FI-20520 Turku, Finland.
Paldanius
Tiina A
TA
Hytönen
Vesa P
VP
Nyholm
Thomas K M
TK
Halling
Katrin K
KK
Huuskonen
Juhani
J
Pentikäinen
Olli T
OT
Rissanen
Kari
K
Slotte
J Peter
JP
Airenne
Tomi T
TT
Salminen
Tiina A
TA
Kulomaa
Markku S
MS
Johnson
Mark S
MS
D000818:Animals; D030161:Avian Proteins / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics; D001360:Avidin / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics; D001391:Azo Compounds / Q000737:chemistry* / Q000494:pharmacology; D001665:Binding Sites; D002152:Calorimetry, Differential Scanning; D002645:Chickens; D018360:Crystallography, X-Ray; D006023:Glycoproteins / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D010047:Ovalbumin / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics; D011487:Protein Conformation; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D030161:Avian Proteins; D001391:Azo Compounds; D006023:Glycoproteins; D008024:Ligands; D001360:Avidin; C001413:HABA; D010047:Ovalbumin