title: Sensitivity enhancement by exchange mediated magnetization transfer of the xenon biosensor signal.
abstract: Hyperpolarized xenon associated with ligand derivatized cryptophane-A cages has been developed as a NMR based biosensor. To optimize the detection sensitivity we describe use of xenon exchange between the caged and bulk dissolved xenon as an effective signal amplifier. This approach, somewhat analogous to 'remote detection' described recently, uses the chemical exchange to repeatedly transfer spectroscopic information from caged to bulk xenon, effectively integrating the caged signal. After an optimized integration period, the signal is read out by observation of the bulk magnetization. The spectrum of the caged xenon is reconstructed through use of a variable evolution period before transfer and Fourier analysis of the bulk signal as a function of the evolution time.
authors.affiliation: Materials Science Division, Lawrence Berkeley National Laboratory, USA; Department of Chemistry, University of California, Berkeley, CA 94720, USA.
authors.forename: Sandra
authors.initials: S
authors.lastname: Garcia
authors.forename: Lana
authors.initials: L
authors.lastname: Chavez
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Lowery
authors.forename: Song-I
authors.initials: SI
authors.lastname: Han
authors.forename: David E
authors.initials: DE
authors.lastname: Wemmer
authors.forename: Alexander
authors.initials: A
authors.lastname: Pines
mesh_terms: D000465:Algorithms; D015374:Biosensing Techniques / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008280:Magnetics; D008832:Microchemistry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014978:Xenon / Q000032:analysis* / Q000737:chemistry*
chemical_list: D014978:Xenon