title: Identification of YhdA as a regulator of the Escherichia coli carbon storage regulation system.
abstract: The Escherichia coli BarA-UvrY two-component system, which controls adaptation via the CsrB and CsrC sRNAs, is induced at the entry of the stationary phase by an unknown stimulus. Using a csrB-lacZ fusion, we demonstrated that the factors RelA, SpoT and LuxS, previously suggested to act on orthologues of this system, have no role in BarA-UvrY induction. However, using a transposon screen, we identified the hypothetical protein YhdA as a new regulator of CsrB and CsrC expression. The YhdA protein is predicted to be membrane-bound and to harbor GGDEF and EAL domains, which, however, are highly divergent from the consensus motifs.
authors.affiliation: Swedish Institute for Infectious Disease Control, Solna, Sweden.
authors.forename: Kristina
authors.initials: K
authors.lastname: Jonas
authors.forename: Henrik
authors.initials: H
authors.lastname: Tomenius
authors.forename: Ute
authors.initials: U
authors.lastname: Römling
authors.forename: Dimitris
authors.initials: D
authors.lastname: Georgellis
authors.forename: Ojar
authors.initials: O
authors.lastname: Melefors
mesh_terms: D002244:Carbon / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000302:isolation & purification* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000502:physiology
chemical_list: C516590:CsrD protein, E coli; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D002244:Carbon