title: Evaluation of a urinary multi-parameter biomarker set for oxidative stress in children, adolescents and young adults.
abstract: The involvement of reactive oxygen species (ROS) and oxidative stress in pediatric diseases is an important concern, but oxidative stress status in healthy young subjects and appropriate methods for its measurement remain unclear. This study evaluated a comprehensive set of urinary biomarkers for oxidative stress in healthy children, adolescents and young adults. Results show that urinary excretion of acrolein-lysine, 8-hydroxy-2'-deoxyguanosine (8-OHdG), nitrite/nitrate and pentosidine were highest in the youngest subjects and decreased to constant levels by early adolescence. Urinary acrolein-lysine, 8-OHdG, nitrite/nitrate and pentosidine showed significant inverse correlations with age, but pyrraline did not change significantly with age. No significant differences in biomarkers were apparent between males and females. Younger subjects grow rapidly and sustain immune activation, and are probably exposed to high concentrations of ROS and nitric oxide. Consequently, they are more vulnerable to oxidation of lipids, proteins, DNA and carbohydrates. Normal reported values in this study are a basis for future studies of disease mechanisms involving oxidative stress and for future trials using antioxidant therapies for oxidative stress-related diseases in the pediatric field.
authors.affiliation: Department of Pediatrics, Faculty of Medical Sciences, University of Fukui, Fukui, 910-1193, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tamura
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tsukahara
authors.forename: Masaki
authors.initials: M
authors.lastname: Ueno
authors.forename: Masayuki
authors.initials: M
authors.lastname: Maeda
authors.forename: Hisako
authors.initials: H
authors.lastname: Kawakami
authors.forename: Kyouichi
authors.initials: K
authors.lastname: Sekine
authors.forename: Mitsufumi
authors.initials: M
authors.lastname: Mayumi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000171:Acrolein / Q000652:urine; D000293:Adolescent; D000328:Adult; D001120:Arginine / Q000031:analogs & derivatives / Q000652:urine; D015415:Biomarkers / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D005260:Female; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006801:Humans; D007223:Infant; D008239:Lysine / Q000031:analogs & derivatives / Q000652:urine; D008297:Male; D009566:Nitrates / Q000652:urine; D009569:Nitric Oxide / Q000737:chemistry; D009573:Nitrites / Q000652:urine; D018384:Oxidative Stress; D017382:Reactive Oxygen Species
chemical_list: D015415:Biomarkers; D017127:Glycation End Products, Advanced; D009566:Nitrates; D009573:Nitrites; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D000171:Acrolein; D000080242:8-Hydroxy-2'-Deoxyguanosine; D001120:Arginine; C062187:pentosidine; D003849:Deoxyguanosine; D008239:Lysine