title: Antiviral activity of HPMPC (cidofovir) against orf virus infected lambs.
abstract: (S)-1-[3-hydroxy-2-(phosphonomethoxy)propyl]cytosine [corrected] (HPMPC, cidofovir, CDV, Vistide) is an acyclic nucleoside analogue with a potent and selective activity against a broad spectrum of DNA viruses including the poxviruses. In this study we present the results of different treatment regimens in lambs experimentally infected with orf virus with different cidofovir formulations prepared in Beeler basis and Unguentum M. Our results show that choice of excipient, concentration of codofovir [corrected] and treatment regimen were all important to the clinical outcome of the therapy. Whilst one particular regimen appeared to exacerbate the lesion, treatment with 1% (w/v) cidofovir cream, prepared in Beeler basis, for 4 consecutive days did result in milder lesions that resolved in milder lesions that resolved [corrected] more quickly than untreated lesions. Furthermore the scabs of the treated animals contained significantly lower amounts of viable virus meaning there should be less contamination of the environment with virus than would normally occur.
authors.affiliation: Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale, Alma Mater Studiorum, Bologna, Italy. ascagliarini@vet.unibo.it
authors.forename: A
authors.initials: A
authors.lastname: Scagliarini
authors.forename: C J
authors.initials: CJ
authors.lastname: McInnes
authors.forename: L
authors.initials: L
authors.lastname: Gallina
authors.forename: F
authors.initials: F
authors.lastname: Dal Pozzo
authors.forename: L
authors.initials: L
authors.lastname: Scagliarini
authors.forename: R
authors.initials: R
authors.lastname: Snoeck
authors.forename: S
authors.initials: S
authors.lastname: Prosperi
authors.forename: J
authors.initials: J
authors.lastname: Sales
authors.forename: J A
authors.initials: JA
authors.lastname: Gilray
authors.forename: P F
authors.initials: PF
authors.lastname: Nettleton
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage*; D000077404:Cidofovir; D003596:Cytosine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D004474:Ecthyma, Contagious / Q000188:drug therapy* / Q000821:virology; D009923:Orf virus / Q000254:growth & development*; D063065:Organophosphonates / Q000008:administration & dosage*; D010232:Paraffin / Q000008:administration & dosage; D012756:Sheep; D012824:Silicic Acid / Q000008:administration & dosage
chemical_list: D000998:Antiviral Agents; D063065:Organophosphonates; C052551:Unguentum Merck; D012824:Silicic Acid; D010232:Paraffin; D003596:Cytosine; D000077404:Cidofovir