[Immune resonance scattering spectral method for the determination of trace IgG].
In pH 7.0 Tris-HCl buffer solution, goat-anti-rabbit IgG is combined with rabbit IgG specifically, and aggregates to form immune complex particles that exhibit three resonance scattering peaks at 330, 400 and 520 nm respectively, and a synchronous scattering peak at 470 nm, in the presence of PEG-20000. The scattering intensity at 470 nm is linear to the rabbit IgG concentration in the range of 1.33 to 133.3 microg x mL(-1). The detection limit is 0.99 microg x mL(-1). The method was applied to the quantitative analysis of rabbit IgG, with satisfactory results.
Deng
Jun-Yao
JY
Department of Resource and Environmental Science, Guangxi Normal University, Guilin 541004, China.
Sun
Shuang-Jiao
SJ
Jiang
Zhi-Liang
ZL
Liang
Ai-Hui
AH
D000818:Animals; D002417:Cattle; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D008027:Light; D011092:Polyethylene Glycols / Q000737:chemistry; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods*
D007074:Immunoglobulin G; D011092:Polyethylene Glycols