title: Monoclonal antibody to the light chain of bovine immunoglobulin.
abstract: Monoclonal antibody IVA-285 (IgG1 isotype) recognizing antigenic determinant on bovine and ovine immunoglobulin light chain was produced and characterized. Western blot analysis of bovine immunoglobulin G (IgG) and immunoglobulin M (IgM) purified from bovine blood serum as well as whole immunoglobulin fractions of bovine and ovine serum with IVA-285 showed a molecular weight in the 24-27 kd range corresponding to the Ig light chain of bovine Ig. IVA-285 recognizes the Ig light chain on Ig+ lymphocyte subpopulation and in the majority of body fluids; however, especially strong reactions were observed in bovine tissues (lymph node follicles, plasma cells, and Ig deposits in various tissues).
authors.affiliation: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic. Jana.Antalikova@savba.sk
authors.forename: Jana
authors.initials: J
authors.lastname: Antalíková
authors.forename: Michal
authors.initials: M
authors.lastname: Simon
authors.forename: Jana
authors.initials: J
authors.lastname: Jankovicová
authors.forename: L'ubica
authors.initials: L
authors.lastname: Horovská
authors.forename: Roman
authors.initials: R
authors.lastname: Dusinský
authors.forename: Svätoslav
authors.initials: S
authors.lastname: Hluchý
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D002417:Cattle; D007074:Immunoglobulin G / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012756:Sheep
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M