Regulation of proliferation and chondrogenic differentiation of human mesenchymal stem cells by laminin-5 (laminin-332).
Laminin-5 (laminin-332) is an important basement membrane protein that regulates cell attachment and motility. Recent studies have shown that laminin-5 is expressed in human mesenchymal stem cells (MSCs) in culture and that the laminin gamma2 chain is transiently expressed in chondrocytes during development. These studies suggest that laminin-5 may be involved in the regulation of chondrogenic differentiation of MSCs. In this study, we examined a possible role of laminin-5 in the proliferation and differentiation of human MSCs. When MSCs were incubated in the presence of a coated or soluble form of laminin-5 in a growth medium, they proliferated more rapidly than nontreated cells, keeping their differentiation potential. On the other hand, laminin-5 potently suppressed the chondrogenic differentiation of MSCs. These activities were mediated mainly by integrin alpha3beta1. However, laminin-5 had no effect on the osteogenic differentiation of MSCs. These results suggest that laminin-5 may contribute to the development of bone tissues by promoting the proliferation and by suppressing the chondrogenic differentiation of MSCs.
Hashimoto
Junko
J
Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, Yokohama, Japan.
Kariya
Yoshinobu
Y
Miyazaki
Kaoru
K
D000469:Alkaline Phosphatase / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism*; D024043:Collagen Type II / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism*; D015675:Osteocalcin / Q000378:metabolism; D010011:Osteocytes / Q000187:drug effects / Q000378:metabolism*; D053495:Osteopontin / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D000097794:Kalinin
D000469:Alkaline Phosphatase; D015815:Cell Adhesion Molecules; D024043:Collagen Type II; D015675:Osteocalcin; D053495:Osteopontin; D011509:Proteoglycans; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000097794:Kalinin