[Determination of three kind of diester diterpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products by HPLC].
OBJECTIVE
To establish an HPLC method for the determination of three kind of diester diterpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products namely mesaconitine, aconitine and hypaconitine.


METHODS
A Zorbax Eclipse XDB C18 column was used, and ammonium acetate solution-acetonitrile as the mobile phase, with a flow rate of 1.0 mL x min(-1) and a detection wavelength of 230 nm.


RESULTS
Mesaconitine, aconitine and hypaconitine, were separated, and the calibration curves of them were in good linearity over the range of 0.035 7-1.784 microg (r = 0.9999), 0.0126-0.632 microg (r = 0.9997) and 0.0334-1.672 microg (r = 0.9997) respectively.


CONCLUSIONS
This method is simple and accurate. It can be used in the identification and quality control of A. carmichaeli.
Liu
Fang
F
Department of Chinese Pharmacy, Beijing University of Traditional Chinese Medicine, Beifing 100102, China. fang68-8@hotmail.com
Yu
Xiang-Hong
XH
Li
Fei
F
Tan
Yu-Yuan
YY
Qiao
Yan-Jiang
YJ
D000157:Aconitine / Q000031:analogs & derivatives / Q000032:analysis; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006358:Hot Temperature; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods
D000470:Alkaloids; C058309:hypaconitine; C019470:mesaconitine; D000157:Aconitine