title: The Bysl gene product, bystin, is essential for survival of mouse embryos.
abstract: Human bystin is a cytoplasmic protein directly binding to trophinin, a cell adhesion molecule potentially involved in human embryo implantation. The present study shows that bystin is expressed in luminal and glandular epithelia in the mouse uterus at peri-implantation stages. In fertilized embryos, bystin was not seen until blastocyst stage. Bystin expression started during hatching and increased in expanded blastocyst. However, bystin apparently disappeared from the blastocyst during implantation. After implantation bystin re-appeared in the epiblast. Targeted disruption of the mouse bystin gene, Bysl, resulted in embryonic lethality shortly after implantation, indicating that bystin is essential for survival of mouse embryos.
authors.affiliation: Cancer Research Center, Burnham Institute for Medical Research, La Jolla, CA, USA.
authors.forename: Rui
authors.initials: R
authors.lastname: Aoki
authors.forename: Nao
authors.initials: N
authors.lastname: Suzuki
authors.forename: Bibhash C
authors.initials: BC
authors.lastname: Paria
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sugihara
authors.forename: Tomoya O
authors.initials: TO
authors.lastname: Akama
authors.forename: Gerhard
authors.initials: G
authors.lastname: Raab
authors.forename: Masaya
authors.initials: M
authors.lastname: Miyoshi
authors.forename: Daita
authors.initials: D
authors.lastname: Nadano
authors.forename: Michiko N
authors.initials: MN
authors.lastname: Fukuda
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000737:chemistry; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D010064:Embryo Implantation; D047108:Embryonic Development / Q000235:genetics*; D004848:Epithelium / Q000737:chemistry; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D013534:Survival; D014162:Transfection; D014599:Uterus / Q000737:chemistry
chemical_list: C504465:Bysl protein, mouse; D015815:Cell Adhesion Molecules