Effects of insulin-like growth factor I on transforming growth factor beta1 induced chondrogenesis of synovium-derived mesenchymal stem cells cultured in a polyglycolic acid scaffold.
The aim of this study was to demonstrate the induction of chondrogenesis by transforming growth factor (TGF)-beta1 from synovium-derived mesenchymal stem cells in a three-dimensional polyglycolic acid (PGA) scaffold, and to evaluate the effects of insulin-like growth factor (IGF)-I on TGF-beta1-induced chondrogenesis. Adult human synovial membranes were obtained from the knees of patients with osteoarthritis or rheumatoid arthritis. Cells were expanded in monolayers, seeded onto a PGA scaffold, and cultured for 4 or 8 weeks in chondrogenic medium containing TGF-beta1 with or without IGF-I. As a control, the cells were cultured in chondrogenic medium without TGF-beta1. The glycosaminoglycan content was quantified using dimethylmethylene blue dye-binding assay, and the DNA content was measured fluorometrically. Histological examination was also performed using safranin-O staining. The expression of mRNA for aggrecan and collagen type II was confirmed by RT-PCR. After 4 weeks of cultivation with TGF-beta1, the cells differentiated to a chondrocytic phenotype, and these chondrogeneses were more potent when cultured for 8 weeks. The combination of IGF-I and TGF-beta1 produced higher amounts of glycosaminoglycan than TGF-beta1 alone at 8 weeks. In conclusions, chondrogenesis from human synovium-derived mesenchymal cells was identified, and IGF-I plays a role in maintaining the extracellular matrix in combination with TGF-beta1.
Sakimura
Koichiro
K
Department of Orthopedic Surgery, Nagasaki University School of Medicine, Nagasaki, Japan.
Matsumoto
Tomoko
T
Miyamoto
Chikara
C
Osaki
Makoto
M
Shindo
Hiroyuki
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000473:pathology; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000187:drug effects* / Q000502:physiology; D004247:DNA / Q000032:analysis; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007719:Knee Joint / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D008875:Middle Aged; D010003:Osteoarthritis / Q000473:pathology; D011100:Polyglycolic Acid / Q000737:chemistry*; D013583:Synovial Membrane / Q000166:cytology / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism / Q000494:pharmacology*
D006025:Glycosaminoglycans; D016212:Transforming Growth Factor beta; D011100:Polyglycolic Acid; D007334:Insulin-Like Growth Factor I; D004247:DNA