Cytoskeletal and nuclear organization in mouse embryos derived from nuclear transfer and ICSI: a comparison of agamogony and syngamy before and during the first cell cycle.
In this study, somatic cell nuclear transfer (SCNT) and intracytoplasmic sperm injection (ICSI) are used as models of agamogony and syngamy, respectively. In order to elucidate the reasons of low efficiency of somatic cell cloning, cytoskeletal and nuclear organization in cloned mouse embryos was monitored before and during the first cell cycle, and compared with the pattern of ICSI zygote. A metaphase-like spindle with alignment of condensed donor chromosomes was assembled within 3 hr after NT, followed by formation of pronuclear-like structures at 3-6 hr after activation, indicating that somatic nuclear remodeling depends on microtubular network organization. The percentage of two (pseudo-) pronuclei in cloned embryos derived from delayed activation was greater than that in immediate activation group (68.5% vs. 30.8%, P<0.01), but similar to that of ICSI group (68.5% vs. 65.5%, P>0.05). The 2-cell rate in NT embryos was significantly lower than that in zygotes produced by ICSI (64.8% vs. 82.5%, P<0.01). Further studies testified that the cloned embryos reached the metaphase of the first mitosis 10 hr after activation, whereas this occurred at 18 hr in the ICSI zygotes. Comparision of the pattern of microfilament assembly in early NT embryos with that in syngamic zygotes suggested that abnormal microfilamental pattern in cloned embryos may threaten subsequent embryonic development. In conclusion, agamogony, in contrast to syngamy, displays some unique features in respect of cytoskeletal organization, the most remarkable of which is that the first cell cycle is initiated ahead distinctly, which probably leads to incomplete organization of the first mitotic spindle, and contributes to low efficiency of cloning.
Zhu
Zi-Yu
ZY
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, and Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.
Jiang
Man-Xi
MX
Yan
Li-Ying
LY
Huang
Jun-Cheng
JC
Lei
Zi-Li
ZL
Jiang
Yan
Y
Ouyang
Ying-Chun
YC
Zhang
Huan-Xiang
HX
Sun
Qing-Yuan
QY
Chen
Da-Yuan
DY
D000818:Animals; D002453:Cell Cycle; D002467:Cell Nucleus / Q000648:ultrastructure*; D019976:Cloning, Organism; D003599:Cytoskeleton / Q000648:ultrastructure*; D004622:Embryo, Mammalian / Q000502:physiology / Q000648:ultrastructure; D047108:Embryonic Development; D005260:Female; D008297:Male; D051379:Mice; D008870:Microtubules / Q000502:physiology; D053652:Nuclear Transfer Techniques; D020554:Sperm Injections, Intracytoplasmic; D015053:Zygote / Q000502:physiology / Q000648:ultrastructure