Efficacy of intracerebral immunization against pseudorabies virus in mice.
To evaluate the efficacy of intracerebral (IC) immunization, mice were immunized with formalin-inactivated pseudorabies virus (PRV) by either subcutaneous (SC) or IC injection, and then 10(6) plaque-forming units of PRV were introduced into the hindleg of the immunized or non-immunized mice by intramuscular injection. The antibody titer in serum was elevated and boosted by additional immunization via both the SC and IC routes, but was higher after IC immunization. Intracerebrally immunized mice were completely protected from mortality and neurological signs, whereas all the non-immunized and 80% of the subcutaneously immunized mice died after developing neurological signs. In mouse models, IC immunization is more effective at inducing a protective immune response against the transneural spread of PRV than SC immunization.
Shin
Jae-Ho
JH
Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9 Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
Sakoda
Yoshihiro
Y
Kim
Jae Hoon
JH
Tanaka
Tomohisa
T
Kida
Hiroshi
H
Kimura
Takashi
T
Ochiai
Kenji
K
Umemura
Takashi
T
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000032:analysis; D002555:Cerebrospinal Fluid / Q000821:virology; D005260:Female; D007114:Immunization; D007279:Injections, Subcutaneous; D051379:Mice; D008807:Mice, Inbred BALB C; D011557:Pseudorabies / Q000401:mortality / Q000473:pathology / Q000517:prevention & control*; D022421:Pseudorabies Vaccines / Q000008:administration & dosage* / Q000276:immunology; D013116:Spinal Cord / Q000473:pathology / Q000821:virology; D015164:Vaccines, Inactivated / Q000008:administration & dosage
D000914:Antibodies, Viral; D000956:Antigens, Viral; D022421:Pseudorabies Vaccines; D015164:Vaccines, Inactivated