title: Antibody responses against HIV in rhesus macaques following combinations of mucosal and systemic immunizations with chimeric alphavirus-based replicon particles.
abstract: Mucosal and systemic transmission of HIV is prevalent. Therefore, mucosal followed by parenteral immunizations with chimeric vs. complete alphavirus-based replicon particles, encoding an HIV envelope glycoprotein, were tested. Female rhesus macaques were immunized intranasally and then intramuscularly. Following the immunizations, enhanced mucosal and systemic antibody responses were detected with the chimeric compared to the complete replicon particles. Although similar proportions of the same peripheral blood monocyte lineage target cells were infected with the chimeric vs. the complete replicon particles, the latter resulted in enhanced expression of the gene of interest, suggesting a possible mechanism of the enhanced immunogenicity.
authors.affiliation: Comparative Medicine, University of California, Davis, California 95616, USA.
authors.forename: Soumi
authors.initials: S
authors.lastname: Gupta
authors.forename: Fengmin
authors.initials: F
authors.lastname: Zhou
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Greer
authors.forename: Harold
authors.initials: H
authors.lastname: Legg
authors.forename: Tony
authors.initials: T
authors.lastname: Tang
authors.forename: Paul
authors.initials: P
authors.lastname: Luciw
authors.forename: Jan
authors.initials: J
authors.lastname: zur Megede
authors.forename: Susan W
authors.initials: SW
authors.lastname: Barnett
authors.forename: John J
authors.initials: JJ
authors.lastname: Donnelly
authors.forename: Derek T
authors.initials: DT
authors.lastname: O'Hagan
authors.forename: John M
authors.initials: JM
authors.lastname: Polo
authors.forename: Michael
authors.initials: M
authors.lastname: Vajdy
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D002678:Chimera / Q000276:immunology; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology*; D005260:Female; D015686:Gene Products, env / Q000276:immunology*; D015483:HIV Antibodies / Q000096:biosynthesis*; D018928:Immunity, Mucosal; D007114:Immunization / Q000379:methods*; D007273:Injections, Intramuscular; D008253:Macaca mulatta / Q000276:immunology*; D012093:Replicon / Q000276:immunology*; D012845:Sindbis Virus / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus
chemical_list: D015686:Gene Products, env; D015483:HIV Antibodies; D054299:env Gene Products, Human Immunodeficiency Virus; C097729:gp140 envelope protein, Human immunodeficiency virus 1