title: [Blocking the gastrin/cholecystokinin-B receptor autocrine loop influences the growth and apoptosis of human gastric cancer cells].
abstract: OBJECTIVE
  To study whether gastrin/cholecystokinin-B (CCK-B) receptor autocrine loop exists in human gastric cancer cells and the effects of gastrin/CCK-B receptor autocrine loop on the growth and apoptosis of human gastric cancer cells.
  
  
  METHODS
  Human gastric cancer cells of the line SGC-7901 were cultured. The expression of gastrin and CCK-B was detected by RT-PCR, immunocytochemistry and radioimmunoassay. Antibody against gastrin was added so as to block the autocrine loop to observe the growth rate, cell cycle and apoptosis by 3-(4.5-dimethylthiazol-z-yl) -2, 5-diphenyl tertrazolium blue (MMT) colorimetric assay and flow cytometry (FCM).
  
  
  RESULTS
  The SGC-7901 cells co-expressed gastrin and CCK-B receptor mRNAs, confirmed by sequencing, thus forming gastrin/the CCK-B receptor autocrine loop. After the blocking of the autocrine loop by antibody against gastrin, the cell growth rate and the number of cells residing in the S-phase of the cell cycle decreased and the apoptotic rate increased in an antibody concentration-dependent manner.
  
  
  CONCLUSIONS
  Human gastric cancer SGC-7901 cells possess the gastrin/the CCK-B receptor autocrine loop. Blocking the autocrine loop inhibits the cell proliferation and promotes the cell apoptosis.
authors.affiliation: Department of Medical Oncology, Molecular Biology Laboratory, Hospital affiliated to Guiyang Medical College, Guiyang 550004, China.
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Wang
authors.forename: Jian-jiang
authors.initials: JJ
authors.lastname: Zhou
authors.forename: Xi-chun
authors.initials: XC
authors.lastname: Zhou
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Yang
mesh_terms: D000183:Actihaemyl; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D019898:Autocrine Communication; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005755:Gastrins / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D043887:Receptor, Cholecystokinin B / Q000096:biosynthesis*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D005755:Gastrins; D012333:RNA, Messenger; D043887:Receptor, Cholecystokinin B; D000183:Actihaemyl