[The significance of DNA-binding inhibitor 4 promoter methylation status in acute leukemia].
OBJECTIVE
To investigate the relationship between promoter methylation of inhibitor of DNA binding 4 (Id4) gene and acute leukemia.


METHODS
We detected the status of promoter methylation of Id4 gene in peripheral blood, peripheral blood stem cell transplantation (PBSC) and bone marrow samples from health donors, bone marrow cells from leukemia patients and leukemia cell lines with MS-PCR methods.


RESULTS
The frequency of Id4 promoter methylation was 21/25 in acute myeloid leukemia (84%), 12/14 in acute lymphoblastic leukemia (86%), 8/8 in relapse acute leukemias and 2/2 in leukemia cell lines whereas no methylation was detected in peripheral blood, PBSC and bone marrow samples from healthy donors. In addition, Hek937 cell line was unmethylated.


CONCLUSIONS
Id4 promoter methylation was detected only in acute leukemia patients and leukemia cell lines and it might play a role in leukemia genesis.
Zhao
Yu
Y
Department of Hematology, General Hospital of PLA, Beijing 100853, China.
Yu
Li
L
Wang
Quan-shun
QS
Li
Hong-hua
HH
Bo
Jian
J
Dou
Li-ping
LP
D019175:DNA Methylation; D006801:Humans; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics / Q000378:metabolism*; D020014:K562 Cells / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D020298:U937 Cells / Q000378:metabolism
C490128:ID4 protein, human; D051796:Inhibitor of Differentiation Proteins