Stripping voltammetric quantification of the anti-diabetic drug glipizide in bulk form and pharmaceutical formulation.
The electrochemical behavior of glipizide at the hanging mercury drop electrode (HMDE) was studied in B-R universal buffers of pH 1.7-11. The voltammograms exhibited a well-defined 4-electron irreversible cathodic peak which attributed to reduction of the two C=N of the pyrazine ring of glipizide molecule. Glipizide was found to has an interfacial adsorptive character onto the mercury electrode surface. A monolayer surface coverage of 1.02x10(-10)mol cm(-2) was estimated and hence each adsorbed glipizide molecule occupied an area of 1.63 nm(2) onto the mercury electrode surface. A simple and precise square-wave adsorptive cathodic stripping (SWAdCS) voltammetric procedure was described for quantification of bulk glipizide with a limit of detection of 1.5x10(-10)M and a limit of quantitation of 5x10(-10)M. The proposed procedure was successfully applied for quantitation of glipizide in its pharmaceutical formulation (Minidiab tablets) without interference from excipients.
Ghoneim
E M
EM
Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt. enassmmghoneim@hotmail.com
El-Attar
M A
MA
Hammam
E
E
Khashaba
P Y
PY
D002021:Buffers; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005913:Glipizide / Q000097:blood* / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007004:Hypoglycemic Agents / Q000097:blood* / Q000737:chemistry*; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000097:blood / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets
D002021:Buffers; D007004:Hypoglycemic Agents; D004364:Pharmaceutical Preparations; D013607:Tablets; D005913:Glipizide