title: Differential gene expression in periportal and perivenous mouse hepatocytes.
abstract: Hepatocytes located in the periportal and perivenous zones of the liver lobule show remarkable differences in the levels and activities of various enzymes and other proteins. To analyze global gene expression patterns of periportal and perivenous hepatocytes, enriched populations of the two cell types were isolated by combined collagenase/digitonin perfusion from mouse liver and used for microarray analysis. In total, 198 genes and expressed sequences were identified that demonstrated a >/= 2-fold difference in expression between hepatocytes from the two different zones of the liver. A subset of 20 genes was additionally analyzed by real-time RT-PCR, validating the results obtained by the microarray analysis. Several of the differentially expressed genes encoded key enzymes of intermediary metabolism, including those involved in glycolysis and gluconeogenesis, fatty acid degradation, cholesterol and bile acid metabolism, amino acid degradation and ammonia utilization. In addition, several enzymes of phase I and phase II of xenobiotic metabolism were differentially expressed in periportal and perivenous hepatocytes. Our results confirm previous findings on metabolic zonation in liver, and extend our knowledge of the regulatory mechanisms at the transcriptional level.
authors.affiliation: Institute of Pharmacology and Toxicology, Department of Toxicology, University of Tuebingen, Germany.
authors.forename: Albert
authors.initials: A
authors.lastname: Braeuning
authors.forename: Carina
authors.initials: C
authors.lastname: Ittrich
authors.forename: Christoph
authors.initials: C
authors.lastname: Köhle
authors.forename: Stephan
authors.initials: S
authors.lastname: Hailfinger
authors.forename: Michael
authors.initials: M
authors.lastname: Bonin
authors.forename: Albrecht
authors.initials: A
authors.lastname: Buchmann
authors.forename: Michael
authors.initials: M
authors.lastname: Schwarz
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015971:Gene Expression Regulation, Enzymologic; D005943:Gluconeogenesis / Q000235:genetics; D006019:Glycolysis / Q000235:genetics; D006503:Hepatic Veins / Q000166:cytology*; D022781:Hepatocytes / Q000378:metabolism*; D008099:Liver / Q000098:blood supply / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008954:Models, Biological; D011169:Portal Vein / Q000166:cytology*; D011489:Protein Denaturation; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D015262:Xenobiotics; D000641:Ammonia; D002784:Cholesterol