Post-transcriptional regulation of the xynA expression by a novel mRNA binding protein, XaiF.
XaiF, a novel 32kDa protein encoded by the ORF located in the immediate downstream of the xynA gene of Bacillus stearothermophilus No. 236, was identified to be the xylanase-specific trans-activator. In this study, the positive effect of XaiF was confirmed to be xylanase-specific, and the results from Northern blot and in vitro transcription assays showed that the XaiF increased the xynA transcripts at post-transcriptional step. Moreover, analysis of the mRNA decay rate led to the assertion that the XaiF functions to stabilize the xynA mRNA. Intriguingly, in vitro RNA-protein binding assay and analysis using gst-xynA 3'-UTR chimeric gene constructs demonstrated that the XaiF stabilizes xynA mRNA by direct binding onto the 3'-UTR of the mRNA.
Jeong
Mi-Young
MY
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Lee
Eung-Ryoung
ER
Yun
Cheol-Won
CW
Cho
Ssang-Goo
SG
Choi
Yong-Jin
YJ
D001426:Bacterial Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D001411:Geobacillus stearothermophilus / Q000502:physiology*; D039381:RNA Cap-Binding Proteins / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology*
D001426:Bacterial Proteins; D039381:RNA Cap-Binding Proteins