A genetically encoded infrared probe.
An orthogonal tRNA/aminoacyl-tRNA synthetase pair has been evolved that makes it possible to selectively and efficiently incorporate para-cyanophenylalanine (pCNPhe) into proteins in E. coli at sites specified by the amber nonsense codon, TAG. Substitution of pCNPhe for histidine-64 in myoglobin (Mb) affords a sensitive vibrational probe of ligand binding. This methodology provides a useful infrared reporter of protein structure, biomolecular interactions, and conformational changes.
Schultz
Kathryn C
KC
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. schultz@scripps.edu
Supekova
Lubica
L
Ryu
Youngha
Y
Xie
Jianming
J
Perera
Roshan
R
Schultz
Peter G
PG
D008958:Models, Molecular; D015335:Molecular Probes; D017550:Spectroscopy, Fourier Transform Infrared
D015335:Molecular Probes