[Studies on plantlet regeneration and propagation of Gentiana stramines].
OBJECTIVE
To provide scientific basis for large scale production by studying the technique of tissue culture of Gentiana stramines.


METHODS
Callus was induced from germ-free stem segment of G. stramines on a MS medium supplemented with different hormones.


RESULTS
The MS medium with 0.5-1.0 mg x L(-1) BA and 0.5 mg x L(-1) NAA was suitable for the induction and proliferation of cluster bads. MS medium with 1.0 mg x L(-1) IAA and 3 mg x L(-1) BA was suitable for the induction of calli. MS medium with 1.0 mg x L(-1) IAA and 2.0-3.0 mg x L(-1) BA was suitable for the subculture of calli. MS medium with 2.0 mg x L(-1) BA and 0.5 mg x L(-1) NAA was suitable for the differentiation of calli.


CONCLUSIONS
Aseptic seeding of G. stramines can be quickly propagated by shoot culture.
Liu
Li-sha
LS
Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China.
Zhang
Xi-ling
XL
Wang
Lan
L
Zhang
Yan-hong
YH
D003470:Culture Media; D030012:Gentiana / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D012038:Regeneration; D036226:Seedlings / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D010937:Plant Growth Regulators