Three types of cilia including a novel 9+4 axoneme on the notochordal plate of the rabbit embryo.
Motile monocilia play a pivotal role in left-right axis determination in mouse and zebrafish embryos. Cilia with 9+0 axonemes localize to the distal indentation of the mouse egg cylinder ("node"), while Kupffer's vesicle cilia in zebrafish show 9+2 arrangements. Here we studied cilia in a prototype mammalian embryo, the rabbit, which develops via a flat blastodisc. Transcription of ciliary marker genes Foxj1, Rfx3, lrd, polaris, and Kif3a initiated in Hensen's node and persisted in the nascent notochord. Cilia emerged on cells leaving Hensen's node anteriorly to form the notochordal plate. Cilia lengthened to about 5 mum and polarized from an initially central position to the posterior pole of cells. Electron-microscopic analysis revealed 9+0 and 9+2 cilia and a novel 9+4 axoneme intermingled in a salt-and-pepper-like fashion. Our data suggest that despite a highly conserved ciliogenic program, which initiates in the organizer, axonemal structures may vary widely within the vertebrates.
Feistel
Kerstin
K
University of Hohenheim, Institute of Zoology, Stuttgart, Germany.
Blum
Martin
M
D000818:Animals; D001483:Base Sequence; D019521:Body Patterning; D002923:Cilia / Q000378:metabolism / Q000648:ultrastructure*; D015342:DNA Probes / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009672:Notochord / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure; D020897:Organizers, Embryonic / Q000196:embryology / Q000378:metabolism / Q000648:ultrastructure; D011247:Pregnancy; D011817:Rabbits; D015027:Zebrafish
D015342:DNA Probes; D005819:Genetic Markers