Quantitative analysis of the expression of glycosylphosphatidylinositol-anchored proteins during the maturation of different hematopoietic cell compartments of normal bone marrow.
BACKGROUND
Glycosylphosphatidylinositol-anchored proteins (GPI-AP) are a heterogeneous group of proteins deficiently expressed in patients with paroxysmal nocturnal hemoglobinuria. Up till now, no study has been reported in which the exact patterns of expression of a large number of GPI-AP are quantitatively evaluated in normal bone marrow (BM) cells classified according to their lineage and maturation stage.


METHODS
In the present study, we have quantitatively analyzed the expression of eleven different GPI-AP (CD14, CD16, CD24, CD48, CD52, CD58, CD59, CD66b, CD87, CD109 and CD157) during maturation of the neutrophil, monocytic, erythroid, lymphoid, basophil and plasmacytoid dendritic cells (DC) lineages in normal BM as a frame of reference for the understanding of the abnormal patterns of expression of GPI-AP observed in the BM of PNH patients.


RESULTS
Our results show that expression of most GPI-AP varies during normal BM maturation, different profiles being frequently observed depending on the cell lineage or the GPI-AP analyzed.


CONCLUSIONS
Overall, these results provide a detailed map GPI-AP expression during normal hematopoietic differentiation, which could serve as a basis for the identification and characterization of changes occurring in PNH.
Hernández-Campo
Pilar María
PM
Servicio General de Citometría and Departamento de Medicina and Centro de Investigación del Cáncer, Universidad de Salamanca, Paseo San Vicente 58-182, 37007 Salamanca, Spain.
Almeida
Julia
J
Matarraz
Sergio
S
de Santiago
María
M
Sánchez
María Luz
ML
Orfao
Alberto
A
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology; D001491:Basophils / Q000166:cytology; D001854:Bone Marrow Cells / Q000166:cytology*; D002451:Cell Compartmentation; D002454:Cell Differentiation; D019070:Cell Lineage; D003713:Dendritic Cells / Q000166:cytology; D041905:Erythroid Cells / Q000166:cytology; D005260:Female; D017261:Glycosylphosphatidylinositols / Q000032:analysis*; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology; D009504:Neutrophils / Q000166:cytology
D015703:Antigens, CD; D017261:Glycosylphosphatidylinositols