Carbohydrate microarrays: survey of fabrication techniques.
Carbohydrate microarrays are being developed in order to decipher the information content of the glycome. This postgenomic activity is necessary because of the complexity of protein biosynthesis and post-translational modifications that cannot currently be detected at the genome level. This review looks, in detail, at the experimental approaches that have been taken in the fabrication and preparation of carbohydrate microarrays, glycan arrays and glyco-chips. Tether structures, glycan solution preparation, detection methods and applications have been gathered together in a tabular format.
Culf
Adrian S
AS
Atlantic Cancer Research Institute, Mount Allison University, Université de Moncton, Moncton, Canada. adrianc@canceratl.ca
Cuperlovic-Culf
Miroslava
M
Ouellette
Rodney J
RJ
D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D006801:Humans; D046228:Microarray Analysis; D011134:Polysaccharides / Q000032:analysis
D002241:Carbohydrates; D011134:Polysaccharides