title: A novel depolarizing activity of scorpion venom fraction M1 due to activation of skeletal muscle nicotinic receptors.
abstract: A depolarizing activity following interaction with nicotinic acetylcholine receptors (nAchRs) in skeletal muscle cells, was observed for the first time in the non-toxic venom fraction (M1) of the yellow scorpion Buthus occitanus tunetanus (Bot). The effects of M1 fraction were tested on cultured rat myotubes by recording changes in [Ca2+]i. When applied, M1 (10 microg/mL) induced a transient increase of [Ca2+]i which could be blocked by a prior application of alpha-Bungarotoxin (alpha-Bg-Tx).
authors.affiliation: Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, Tunis BP 74-1002, Tunisia.
authors.forename: Amani
authors.initials: A
authors.lastname: Cheikh
authors.forename: Rym
authors.initials: R
authors.lastname: Benkhalifa
authors.forename: Daniel
authors.initials: D
authors.lastname: Potreau
authors.forename: Guy
authors.initials: G
authors.lastname: Raymond
authors.forename: Mohamed
authors.initials: M
authors.lastname: El Ayeb
authors.forename: Christian
authors.initials: C
authors.lastname: Cognard
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000187:drug effects* / Q000378:metabolism; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology*; D051381:Rats; D011978:Receptors, Nicotinic / Q000187:drug effects* / Q000378:metabolism; D012604:Scorpion Venoms / Q000494:pharmacology*; D012605:Scorpions
chemical_list: D015220:Calcium Channels; D011978:Receptors, Nicotinic; D012604:Scorpion Venoms