title: Serum antioxidant enzyme activities and oxidative stress parameters as possible biomarkers of exposure in veal calves illegally treated with dexamethasone.
abstract: Low doses of the synthetic glucocorticoid dexamethasone (DEX) are often illegally used, alone or in association with steroids and beta-agonists, to improve meat performances in cattle. As it is known that oestrogens and beta-agonists may generate reactive oxygen species (ROS) and induce oxidative stress, the effects of illicit DEX protocols on the antioxidant status and oxidative stress parameters were measured in veal calves. Ten cross-bred male veal calves were given DEX (0.4mg/day administered per os, for 23days or 2mg pro capite, injected intramuscularly on days 14 and 21 after the beginning of the oral DEX administration). Five further animals were used as controls. Blood samples were withdrawn before (T(0)), and 4 (T(1)), 10 (T(2)), 14 (T(3)), 21 (T(4)) and 28 (T(5)) days. Antioxidant enzyme activities (AOEs), the serum antioxidant capacity (SAC) and ROS were measured in sera. Calves orally treated showed a significant increase of both glutathione peroxidase isoforms (P<0.05) and SAC (P<0.05), too. Antioxidant enzymes have already been used as biomarkers (BMs) of response, measured in target or in surrogate tissues. Our results suggest glutathione peroxidase and SAC as possible BMs of illicit oral low-dose administration of DEX in cattle.
authors.affiliation: Dipartimento di Sanità pubblica, Università di Padova, Agripolis Legnaro, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Carletti
authors.forename: Michela
authors.initials: M
authors.lastname: Cantiello
authors.forename: Mery
authors.initials: M
authors.lastname: Giantin
authors.forename: Carlo
authors.initials: C
authors.lastname: Nebbia
authors.forename: Francesca Tiziana
authors.initials: FT
authors.lastname: Cannizzo
authors.forename: Enrico
authors.initials: E
authors.lastname: Bollo
authors.forename: Mauro
authors.initials: M
authors.lastname: Dacasto
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers; D002417:Cattle; D003907:Dexamethasone / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D003907:Dexamethasone; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase