Activated platelets retain their potential to induce osteoclast-like cell formation in murine bone marrow cultures.
Supernatants immediately obtained after platelet activation can induce osteoclast-like cell formation in murine bone marrow cultures. Here we report that activated platelets retain their potential to induce osteoclast-like cell formation over a 3-day period with repeated washing, when co-cultured with murine bone marrow cells. Supernatants obtained from washed platelets 3 days following their activation with thrombin, caused the differentiation of haematopoietic progenitors into osteoclast-like cells. The platelet-derived soluble factor(s) responsible for the induction of osteoclastogenesis can be retained in an ultrafilter with a nominal molecular weight limit of 10 kDa, and loose their activity when incubated at 99 degrees C. Indomethacin, which inhibits cyclooxygenase activity, and osteoprotegerin, a decoy receptor for receptor activator of nuclear factor-kappaB ligand (RANKL), suppressed the formation of osteoclast-like cells in this model. The in vitro findings presented here suggest that activated platelets can induce osteoclast-like cell formation via a prostaglandin and RANKL-dependent mechanism over a time period corresponding to the existence of a blood clot.
Maitz
Philipp
P
Department of Oral Surgery, Medical University of Vienna, Vienna, Austria.
Kandler
Barbara
B
Fischer
Micheal B
MB
Watzek
Georg
G
Gruber
Reinhard
R
D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology; D001854:Bone Marrow Cells / Q000166:cytology*; D002450:Cell Communication / Q000502:physiology; D002454:Cell Differentiation; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D051379:Mice; D010010:Osteoclasts / Q000166:cytology*; D015539:Platelet Activation / Q000502:physiology*; D011453:Prostaglandins / Q000502:physiology; D053245:RANK Ligand / Q000502:physiology; D013917:Thrombin / Q000494:pharmacology
D017077:Culture Media, Conditioned; D011453:Prostaglandins; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; D013917:Thrombin