title: [Effects of ligustrazine on hippocampal dentate gyrus cell proliferation after focal cerebral ischemia in adult rats].
abstract: OBJECTIVE
  To explore the effects of ligustrazine on cell proliferation in hippocampal dentate gyrus subgranular zone (SGZ) after focal cerebral ischemia in adult rats.
  
  
  METHODS
  Middle cerebral artery occlusion (MCAO) model was established in adult rats by placement of an intraluminal filament at the origin of the MCA. Ligustrazine was administered intraperitoneally at a daily dose of 80 mg/kg starting at 2 h after MCAO, and BrdU (50 mg/kg daily) was also injected intraperitoneally starting at 4 h after MCAO. BrdU-positive cells in the SGZ were counted 7, 14 and 24 days after MCAO, respectively.
  
  
  RESULTS
  Compared with sham operation group, ischemic ipsilateral BrdU-positive cells in the ischemic model group increased 7 days after MCAO, reaching the peak on day 14, and decreased on day 21 (P<0.01). The number of ischemic ipsilateral BrdU-positive cells in ligustrazine group was significantly greater than that in the ischemic model group on days 7, 14 and 21 (P<0.01), and maintained the high level on day 21.
  
  
  CONCLUSIONS
  Ligustrazine possesses long lasting effect of promoting cell proliferation in the SGZ after focal cerebral ischemia in adult rats.
authors.affiliation: Research Center for Neuroscience, Key Laboratory of Environment and Genes Related Diseases of Ministry of Education, Medical School of Xi'an Jiaotong University, Xi'an 710061, China. qiufen6688@mail.xjtu.edu.cn
authors.forename: Fen
authors.initials: F
authors.lastname: Qiu
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Peng-bo
authors.initials: PB
authors.lastname: Zhang
authors.forename: Ying-fang
authors.initials: YF
authors.lastname: Tian
authors.forename: Cun-fang
authors.initials: CF
authors.lastname: Qi
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Xin-li
authors.initials: XL
authors.lastname: Xiao
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D018891:Dentate Gyrus / Q000187:drug effects* / Q000473:pathology; D020244:Infarction, Middle Cerebral Artery / Q000503:physiopathology*; D007274:Injections, Intraperitoneal; D008297:Male; D011719:Pyrazines / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D011719:Pyrazines; C017953:tetramethylpyrazine