Lipid peroxidation as a source of oxidative damage in Helicobacter pylori: protective roles of peroxiredoxins.
Oxidative stress conditions lead to enzymatic and non-enzymatic unsaturated fatty acid-initiated lipid peroxidation reactions. One exacerbating product is lipid hydroperoxide (LOOH) which itself promotes formation of several additional peroxyl radicals. Helicobacter pylori mutant strains with disruptions in genes encoding the peroxiredoxins, alkyl hydroperoxide reductase (ahpC) and the bacterioferritin comigratory protein (bcp), were more sensitive than the parent strain to oxidizing agents. These mutant strains were particularly sensitive, compared to the wild type, to killing by the unsaturated fatty acid linolenic acid but were not sensitive to the saturated fatty acid palmitic acid. A double mutant strain (ahpC bcp) accumulated more than 3-fold more lipid peroxides than the parent strain, indicating these peroxiredoxins together play a role in detoxifying lipid peroxides. The level of free iron accumulation, a signature of oxidative stress damage, was correlated specifically to organic peroxide-mediated stress by both in vivo and in vitro approaches. Free iron accumulation and concomitant destruction of [Fe-S] cluster-containing proteins (hydrogenase and aconitase) was correlated to damage mediated by exogenous t-butyl peroxide, or separately to intracellular accumulation of lipid peroxides in mutant strains. A major macromolecular target of accumulating lipid peroxides in H. pylori is DNA, as mutant analysis approaches combined with quantitative DNA fragmentation studies and specific DNA damage assessment (i.e. 8-oxoguanine formation) were used to demonstrate that such damage was especially associated with ahpC and ahpC bcp strains.
Wang
Ge
G
Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
Hong
Yang
Y
Johnson
Michael K
MK
Maier
Robert J
RJ
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology; D016480:Helicobacter pylori / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000378:metabolism; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism* / Q000494:pharmacology; D008954:Models, Biological; D009154:Mutation; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D054464:Peroxiredoxins; D020011:Protective Agents / Q000378:metabolism
D001426:Bacterial Proteins; D007506:Iron-Sulfur Proteins; D008054:Lipid Peroxides; D016877:Oxidants; D020011:Protective Agents; C068491:bacterioferritin comigratory protein, Bacteria; D010544:Peroxidases; D054464:Peroxiredoxins; C508531:ahpC protein, Helicobacter pylori