title: The DEAD-box protein Ded1 unwinds RNA duplexes by a mode distinct from translocating helicases.
abstract: Helicases unwind RNA or DNA duplexes and displace proteins from nucleic acids in an ATP-dependent fashion. To unwind duplexes, helicases typically load onto one of the two nucleic acid strands, usually at a single-stranded region, and then translocate on this strand in a unidirectional fashion, thereby displacing the complementary DNA or RNA. Here we show that the DEAD-box RNA helicase Ded1 unwinds duplexes in a different manner. Ded1 uses the single-stranded region to gain access to the duplex. Strand separation is directly initiated from the duplex region and no covalent connection between the single strand and the duplex region is required. This new type of helicase activity explains observations with other DEAD-box proteins and may be the prototype for duplex-unwinding reactions in RNA metabolism.
authors.affiliation: Department of Biochemistry and Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Ave., Cleveland, Ohio 44106, USA.
authors.forename: Quansheng
authors.initials: Q
authors.lastname: Yang
authors.forename: Eckhard
authors.initials: E
authors.lastname: Jankowsky
mesh_terms: D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D004277:DNA, Single-Stranded; D012330:RNA, Double-Stranded; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D004247:DNA; C492152:DED1 protein, S cerevisiae; D053487:DEAD-box RNA Helicases