Prevalence and sequence variants of IS481 in Bordetella bronchiseptica: implications for IS481-based detection of Bordetella pertussis.
We report the prevalence in Bordetella bronchiseptica of IS481, a frequent target for diagnosis of Bordetella pertussis, as approximately 5%. However, PCR amplicons of the predicted size were detectable in 78% of IS481-negative strains. Our results suggest that PCR targeting IS481 may not be sufficiently specific for reliable identification of B. pertussis.
Register
Karen B
KB
Respiratory Diseases of Livestock Research Unit, USDA/ARS/National Animal Disease Center, P.O. Box 70, 2300 Dayton Road, Ames, IA 50010, USA. kregiste@nadc.ars.usda.gov
Sanden
Gary N
GN
D000818:Animals; D001483:Base Sequence; D001885:Bordetella Infections / Q000175:diagnosis / Q000382:microbiology* / Q000662:veterinary; D016950:Bordetella bronchiseptica / Q000235:genetics* / Q000302:isolation & purification; D001886:Bordetella pertussis / Q000235:genetics* / Q000302:isolation & purification; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D005188:False Negative Reactions; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014917:Whooping Cough / Q000175:diagnosis* / Q000382:microbiology
D004251:DNA Transposable Elements; D004269:DNA, Bacterial