Partial purification of mannosylphosphorylundecaprenol synthase from Micrococcus luteus: a useful enzyme for the biosynthesis of a variety of mannosylphosphorylpolyisoprenol products.
Membrane fractions from Micrococcus luteus catalyze the transfer of mannose from GDP-mannose to mono- and dimannosyldiacylglycerol, mannosylphosphorylundecaprenol (Man-P-Undec), and a membrane-associated lipomannan. This chapter describes the detergent solubilization, partial purification, and properties of Man-P-Undec synthase. The mobility of the mannosyltransferase activity on sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicates that the enzyme is a polypeptide with a molecular weight of approx 30.7 kDa. Utilizing the broad specificity of the bacterial mannosyltransferase provides a useful approach for the enzymatic synthesis of a wide variety of Man-P-polyisoprenol products.
Rush
Jeffrey S
JS
Department of Molecular and Cellular Biochemistry, University of Kentucky, College of Medicine, Lexington, KY, USA.
Waechter
Charles J
CJ
D002847:Chromatography, Agarose / Q000379:methods; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D003902:Detergents / Q000737:chemistry; D008364:Mannosyltransferases / Q000302:isolation & purification / Q000378:metabolism; D016982:Micrococcus luteus / Q000201:enzymology*; D008967:Molecular Biology / Q000379:methods*; D009113:Muramidase / Q000737:chemistry; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D012995:Solubility; D013379:Substrate Specificity
D003902:Detergents; D011106:Polyisoprenyl Phosphates; D008364:Mannosyltransferases; C020563:GDPmannose - undecaprenyl-phosphate mannosyltransferase; D009113:Muramidase