title: Efficacy of intracerebral immunization against pseudorabies virus in mice.
abstract: To evaluate the efficacy of intracerebral (IC) immunization, mice were immunized with formalin-inactivated pseudorabies virus (PRV) by either subcutaneous (SC) or IC injection, and then 10(6) plaque-forming units of PRV were introduced into the hindleg of the immunized or non-immunized mice by intramuscular injection. The antibody titer in serum was elevated and boosted by additional immunization via both the SC and IC routes, but was higher after IC immunization. Intracerebrally immunized mice were completely protected from mortality and neurological signs, whereas all the non-immunized and 80% of the subcutaneously immunized mice died after developing neurological signs. In mouse models, IC immunization is more effective at inducing a protective immune response against the transneural spread of PRV than SC immunization.
authors.affiliation: Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9 Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Shin
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sakoda
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Tanaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
authors.forename: Takashi
authors.initials: T
authors.lastname: Kimura
authors.forename: Kenji
authors.initials: K
authors.lastname: Ochiai
authors.forename: Takashi
authors.initials: T
authors.lastname: Umemura
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000032:analysis; D002555:Cerebrospinal Fluid / Q000821:virology; D005260:Female; D007114:Immunization; D007279:Injections, Subcutaneous; D051379:Mice; D008807:Mice, Inbred BALB C; D011557:Pseudorabies / Q000401:mortality / Q000473:pathology / Q000517:prevention & control*; D022421:Pseudorabies Vaccines / Q000008:administration & dosage* / Q000276:immunology; D013116:Spinal Cord / Q000473:pathology / Q000821:virology; D015164:Vaccines, Inactivated / Q000008:administration & dosage
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D022421:Pseudorabies Vaccines; D015164:Vaccines, Inactivated