[Effect of zhigancao decoction containing serum on transient outward potassium current in isolated single ventricular myocyte of rabbits].
OBJECTIVE
To investigate the effect of Zhigancao Decoction (ZD) containing serum on transient outward potassium current (I(to)) in isolated single ventricular myocyte of rabbits.


METHODS
Single rabbit ventricular myocytes were isolated and divided into 6 groups, the normal group, the non-drug-containing serum group, and the ZD groups, they were treated with normal extracellular fluid alone or with ZD containing serum recording technique was used to observe the changes of I(to).


RESULTS
After treated with 5%, 10%, 20% and 40% ZD containing serum, the I(to) was inhibited and its peak value dropped from (16.1 +/- 1.4) PA/PF to (13.9 +/-1.5) PA/PF, (11.8 +/- 1.9) PA/PF, (8.3 +/- 1.5) PA/PF and (8.2 +/- 1.2) PA/PF respectively, and the effect could be eliminated by elution.


CONCLUSIONS
ZD containing serum could inhibit the I(to) in isolated ventricular myocytes of rabbits in a concentration-dependent manner, which may be the mechanism of antiarrhythmic action of ZI).
Zhou
Cheng-zhi
CZ
Department of Cardiology, Cardiology, Hubei Provincial Hospital of TCM, Wuhan. acheng8341@mail.china.com
Zhang
Dao-liang
DL
Wang
Teng
T
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004594:Electrophysiology; D006352:Heart Ventricles / Q000166:cytology; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015221:Potassium Channels / Q000378:metabolism*; D011817:Rabbits; D011897:Random Allocation; D044967:Serum
D000889:Anti-Arrhythmia Agents; D004365:Drugs, Chinese Herbal; D015221:Potassium Channels; C526719:zhigancao decoction