Comparative genomic hybridization by microarray for the detection of cytogenetic imbalance.
Chromosomal abnormalities often result in the improper dosage of genes in a particular chromosome or chromosome segment, which may cause specific and complex clinical phenotypes. Comparative genomic hybridization by microarray (array CGH) is a high-throughput and high-resolution method for the detection of microscopic and submicroscopic chromosome abnormalities, some of which may not be detectable by conventional cytogenetic techniques. In addition, with the human genome sequenced and publicly available, array CGH allows for the direct correlation between chromosomal anomalies and genomic sequence. Properly constructed, microarrays have the potential to be a valuable tool for the detection of chromosomal abnormalities in cancer and genetic disease.
Jarmuz
Malgorzata
M
Health Research and Education Center, Washington State University, Spokane, WA, USA.
Ballif
Blake C
BC
Kashork
Catherine D
CD
Theisen
Aaron P
AP
Bejjani
Bassem A
BA
Shaffer
Lisa G
LG
D002460:Cell Line; D002869:Chromosome Aberrations; D025063:Chromosome Disorders / Q000175:diagnosis* / Q000235:genetics; D020732:Cytogenetic Analysis / Q000379:methods; D005260:Female; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D007621:Karyotyping / Q000379:methods; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010641:Phenotype