Fatal hemorrhagic diathesis associated with mild factor IX deficiency in pl/J mice.
Surgical implantation of devices into the abdomen of PL/J mice was associated with fatal hemorrhage at 9 to 11 d after surgery. Coagulation profiles were evaluated to determine the underlying cause of this effect. The mean activated partial thromboplastin time (aPTT) of untreated PL/J mice was significantly higher than that of BALB/cByJ and C57BL/6J strains. The addition of human plasmas deficient in factors VIII, XI, or XII, prekallikrein, or high molecular-weight kininogen corrected the elevated aPTT of PL/J mice, but correction did not occur when factor IX-deficient human plasma was added. When compared to an assigned factor IX activity of 100% for pooled plasma from BALB/cByJ mice, C57BL/6J and PL/J mice revealed percent activities of 67% and 16%, respectively. PL/J mice could represent a new model for the study of pathogenesis and therapy of mild factor IX deficiency that is expressed and becomes clinically apparent secondary to major surgery.
Trammell
Rita A
RA
Department of Pharmacology, Southern Illinois University School of Medicine, Springfield, Illinois, USA. rtrammell@siumed.edu
Brooks
Marjory
M
Cox
Lisa
L
Ding
Ming
M
Wagenknecht
Dawn R
DR
Rehg
Jerold E
JE
McIntyre
John A
JA
Toth
Linda A
LA
D000818:Animals; D001522:Behavior, Animal; D001777:Blood Coagulation; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D005164:Factor IX / Q000235:genetics; D002836:Hemophilia B / Q000097:blood / Q000150:complications*; D006474:Hemorrhagic Disorders / Q000097:blood / Q000209:etiology*; D007132:Immunoglobulin Isotypes / Q000097:blood; D020007:Implants, Experimental / Q000009:adverse effects; D051379:Mice; D008815:Mice, Inbred Strains; D011183:Postoperative Complications
D007132:Immunoglobulin Isotypes; D005164:Factor IX