Evaluation of the siderophores production by Pseudomonas aeruginosa PSS.
Siderophores are compounds secreted under low iron stress, that act as a specific ferric iron chelate agents and due to their potentialities in the biological control of phytopathogenic fungi and bacteria, their study has been stimulated in recent years. Siderophores produced by different Pseudomonas species have been widely studied as biological agents and it is an alternative to take into account in the control of phytopathogenic microorganisms in agriculture. The purpose of this paper was to study the influence of some culture medium, and the iron concentration in the production of this metabolite. The experiments were carried out in a conventional batch system in succinate, glucose and glutamate medium. The highest metabolite concentration was obtained in glucose and glutamate medium. The increase of Fe(III) concentration, had a negative effect in siderophores production, especially above 10 microM. The evaluation of the studied media led to the conclusion that it is possible to increase the production of this metabolite by the strain of Pseudomonas aeruginosa PSS, in a glutamate medium without iron addition.
Díaz de Villegas
María Elena
ME
Dpto. de Bioquímica, Cuban Institute for Research on Sugar Cane by-Products Vía Blanca 804 y Carretera Central, P.O. Box 4026, La Habana, Cuba. villegas@icidca.edu.cu
Villa
Pilar
P
Frías
Alina
A
D003470:Culture Media / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D018698:Glutamic Acid / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D017262:Siderophores / Q000096:biosynthesis*; D019802:Succinic Acid / Q000494:pharmacology*
D003470:Culture Media; D017262:Siderophores; D018698:Glutamic Acid; D019802:Succinic Acid; D007501:Iron; D005947:Glucose