title: Proteomic analysis of nipple aspirate fluid throughout the menstrual cycle in healthy pre-menopausal women.
abstract: A proteomic approach to nipple aspiration fluid (NAF) has been used in a number of studies comparing women with breast cancer and healthy women. However, to make useful comparisons between women with breast cancer and healthy women it is necessary to establish whether there is physiological variation in the proteomic profiles of NAF. The purpose of this study was, for the first time, to examine how the proteomic profile of NAF using surface-enhanced laser desorption ionisation time-of-flight mass spectrometry varies across the menstrual cycle in healthy pre-menopausal women. Twelve women were recruited and nipple aspiration was carried out weekly from both breasts of each subject for two menstrual cycles. Matching serum samples for luteinising hormone, follicle stimulating hormone and oestradiol were obtained at each aspiration attempt. Statistically significant peaks were found for three healthy volunteers (p < 0.05). However, the peaks that varied across the menstrual cycle were different from one healthy volunteer to another and the differences were small compared with the large variation in proteomic profiles between healthy volunteers. This study provides proof of concept that the NAF proteomic profile does not vary substantially during the menstrual cycle and that therefore it is valid to compare NAF profiles from pre-menopausal women that have been taken at different stages in the menstrual cycle.
authors.affiliation: Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK.
authors.forename: Jillian
authors.initials: J
authors.lastname: Noble
authors.forename: R Sascha
authors.initials: RS
authors.lastname: Dua
authors.forename: Imogen
authors.initials: I
authors.lastname: Locke
authors.forename: Rosalind
authors.initials: R
authors.lastname: Eeles
authors.forename: Gerald P H
authors.initials: GP
authors.lastname: Gui
authors.forename: Clare M
authors.initials: CM
authors.lastname: Isacke
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D001707:Biopsy, Needle; D001826:Body Fluids; D001943:Breast Neoplasms / Q000378:metabolism*; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood; D008597:Menstrual Cycle; D009558:Nipples / Q000737:chemistry*; D017697:Premenopause; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D020543:Proteome; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone