Stacking the deck: double-tiled DNA microarrays.
Microarrays-high-throughput platforms for analyzing the gene expression and features of total genomic DNA, among other applications-are gaining in popularity as researchers discover ever more uses for their unbiased and broad feature sets. At present, microarray analyses are limited by the number of individual features that can be placed on each array. Here we describe a double-tiling method that significantly increases the number of sequences present on an array, and we show that successful transcriptional profiling is possible and straightforward with such arrays. With this method, we and others can save money and precious samples by using fewer arrays to cover a region, or can carry out investigations at significantly higher resolution without incurring prohibitive costs or increasing the amount of sample required for the experiment.
Wheelan
Sarah J
SJ
The High Throughput Biology Center and Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, 733 North Broadway, BRB Suite 331 Baltimore, Maryland 21205, USA.
Martínez-Murillo
Francisco
F
Irizarry
Rafael A
RA
Boeke
Jef D
JD
D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000187:drug effects / Q000235:genetics; D004867:Equipment Design; D005690:Galactose / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D005947:Glucose / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012680:Sensitivity and Specificity; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
D002232:Carbocyanines; D004247:DNA; D005947:Glucose; D005690:Galactose