title: Should human chondrocytes fly? The impact of electromagnetic irradiation on chondrocyte viability and implications for their use in tissue engineering.
abstract: A significant logistic factor as to the successful clinical application of the autologous tissue engineering concept is efficient transportation: the donor cells need to be delivered to tissue processing facilities which in most cases requires air transportation. This study was designed to evaluate how human chondrocytes react to X-ray exposure. Primary cell cultures were established, cultured, incubated and exposed to different doses and time periods of radiation. Subsequently, quantitative cell proliferation assays were done and qualitative evaluation of cellular protein production were performed. Our results show that after irradiation of chondrocytes with different doses, no significant differences in terms of cellular viability occurred compared with the control group. These results were obtained when chondrocytes were exposed to luggage transillumination doses as well as exposure to clinically used radiation doses. Any damage affecting cell growth or quality was not observed in our study. However, information about damage of cellular DNA remains incomplete.
authors.affiliation: Division of Plastic, Hand and Reconstructive Surgery, University Hospital Zurich, Raemistrasse 100, 8091 Zurich, Switzerland. christian.koehler@usz.ch
authors.forename: C
authors.initials: C
authors.lastname: Koehler
authors.forename: A D
authors.initials: AD
authors.lastname: Niederbichler
authors.forename: T
authors.initials: T
authors.lastname: Scholz
authors.forename: B
authors.initials: B
authors.lastname: Bode
authors.forename: J
authors.initials: J
authors.lastname: Roos
authors.forename: F J
authors.initials: FJ
authors.lastname: Jung
authors.forename: S P
authors.initials: SP
authors.lastname: Hoerstrup
authors.forename: J P
authors.initials: JP
authors.lastname: Hellermann
authors.forename: V
authors.initials: V
authors.lastname: Wedler
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D020219:Chondrogenesis / Q000502:physiology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D006801:Humans; D011829:Radiation Dosage; D023822:Tissue Engineering / Q000379:methods*; D014965:X-Rays