[Nitric oxide regulates c-fos expression in osteoblastic cells induced by wall-shear].
To observe regulation of nitric oxide on c-fos expression in osteoblastic cells in response to changes in wall-shear stress in vitro.



Isolated and purified osteoblastic cells from the calvaria of newborn SD rats were cultured and passaged. The third generation cells, pre-treated with 10% FBS DMEM, 0.3 mmol/L L-NMMA DMEM and 0.1 mmol/L SNP DMEM separately, were subjected to wall-shear stress of 1.2 Pa. Gene expression of the c-fos and NOS activity were studied before (0 min) and 10 min, 15 min, 30 min, 60 min after treated with wall-shear stress.



The expression of c-fos mRNA was increased transiently after application of 1.2 Pa wall-shear stress in osteoblastic cells and peaked at 15 min. The expression of c-fos mRNA was decreased after pre-application with L-NMMA and increased after use of SNP.



Changes in the osteoblastic cells mechanical environment may cause a dramatic induction of NO and c-fos expression.
Zhu
Fu-dong
FD
Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology of Hospital, College of Medicine Zhejiang University, Hangzhou 310006, China.
Zhao
Shi-fang
SF
Tong
Xiao-yan
XY
D000818:Animals; D000831:Animals, Newborn; D001696:Biomechanical Phenomena; D002478:Cells, Cultured; D005260:Female; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000235:genetics; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000166:cytology; D013314:Stress, Mechanical
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase