title: [Spectroscopic studies on the interactions of a hexadeca-carboxy zinc phthalocyanine and serum albumin and preparation of the bioconjugates].
abstract: The interactions of the new photosensitizer {2, 3, 9, 10, 16, 17, 23, 24-octakis[(3,5-dicarboxy)phenoxy]-phthalocyaninato}zinc(II) [ZnPc(COOH)16] and serum albumins (BSA and HSA) were investigated by absorption and fluorescence spectroscopy. The binding constants were found to be 2.25-2.94 x 10(6) L x mol(-1). The 1 : 1 ZnPc (COOH)16-BSA and ZnPc (COOH)16-BSA conjugates were also prepared by incubating a mixture of ZnPc(COOH)16 and the corresponding serum albumin in 2 : 1 mole ratio followed by separation by gel permeation chromatography. The Q band absorption and fluorescence emission of ZnPc(COOH)16 are slightly red-shifted in the conjugates, and the spectral features suggest that the phthalocyanine exists predominantly in monomeric form in the conjugates.
authors.affiliation: College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China.
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Chen
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Huang
authors.forename: Feng-Ran
authors.initials: FR
authors.lastname: Liu
authors.forename: Rui-Qing
authors.initials: RQ
authors.lastname: Sun
authors.forename: Dennis K P
authors.initials: DK
authors.lastname: Ng
mesh_terms: D000818:Animals; D002417:Cattle; D006801:Humans; D007211:Indoles / Q000737:chemistry* / Q000378:metabolism; D054833:Isoindoles; D007700:Kinetics; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D017967:Zinc Compounds
chemical_list: D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D012709:Serum Albumin; D017967:Zinc Compounds; C549929:ZnPc(COOH)16; C052159:Zn(II)-phthalocyanine; D012710:Serum Albumin, Bovine