Vasorelaxant effect of the rootbark extract of Paeonia moutan on isolated rat thoracic aorta.
The vascular relaxant effect of the rootbark extract of Paeonia moutan was evaluated in isolated rat thoracic aorta. The methanolic extract of the rootbark showed a vasorelaxant activity in rat aortic preparations precontracted with 0.3 microM phenylephrine (IC50 value: 16.8 microg/mL). The activity-guided fractionation of the extract led to the isolation of five active principles such as paeoniflorin (1), paeonidanin (2), methylpaeoniflorin (4), tetragalloylglucose (5) and pentagalloylglucose (6), and these active ingredients potently relaxed phenylephrine-induced contraction of rat aortic preparations in a concentration-dependent manner (IC50 values: 19.4, 7.9, 10.1, 5.1 and 3.6 microM, respectively). These results suggest that pinane glycosides and galloylglucoses might be the components responsible for the vasorelaxant properties of the rootbark extract of P. moutan, and their vasorelaxant effects may be mediated through increases in the release of nitric oxide from endothelial cells.
Yoo
Mi Young
MY
Korea Research Institute of Chemical Technology, Daejeon, Korea.
Lee
Byung Ho
BH
Choi
Yeon Hee
YH
Lee
Jung Won
JW
Seo
Jee Hee
JH
Oh
Kwang-Seok
KS
Koo
Hyun-Na
HN
Seo
Ho Won
HW
Yon
Gyu Hwan
GH
Kwon
Dae Young
DY
Kim
Young Sup
YS
Ryu
Shi Yong
SY
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D029596:Paeonia; D010656:Phenylephrine; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000187:drug effects*; D014665:Vasodilator Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D010936:Plant Extracts; D014665:Vasodilator Agents; D010656:Phenylephrine