title: Gliadin characterization by SANS and gliadin nanoparticle growth modelization.
abstract: Nanosized colloidal carriers can ensure a controlled and targeted therapeutic substances delivery. The original contribution of this work was to use biopolymers of vegetable source, which are an interesting alternative to synthetic polymers. The aim of this study was to prepare submicronic particles from wheat proteins: Gliadins extracted from gluten. The carrier preparation was based on the desolvatation of the macromolecules by a couple solvent/non-solvent of the proteins. In a first step, it was of interest to elucidate the gliadin macromolecular conformation in order to understand the mechanism of nanoparticle formation. The experimental work was based on SANS experiments. Because the size of the colloidal particle suspension is an important parameter to monitor, the modelization of the particle growth was thoroughly studied. Furthermore, it was observed that the determination of the solubility parameters of the proteins allowed optimization of the size of the particles. From those previous experimental results it can be concluded that there is a correlation between the protein conformation in the solvent and the size of the nanoparticles (NP).
authors.affiliation: Université de Rouen, 1 allée des Tilleuls, 92330 Sceaux, France.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Orecchioni
authors.forename: Cécile
authors.initials: C
authors.lastname: Duclairoir
authors.forename: Denis
authors.initials: D
authors.lastname: Renard
authors.forename: Evelyne
authors.initials: E
authors.lastname: Nakache
mesh_terms: D003198:Computer Simulation; D003460:Crystallization / Q000379:methods*; D004337:Drug Carriers / Q000737:chemistry*; D005903:Gliadin / Q000737:chemistry* / Q000648:ultrastructure*; D046911:Macromolecular Substances; D008422:Materials Testing; D008956:Models, Chemical; D008958:Models, Molecular; D008968:Molecular Conformation; D046912:Multiprotein Complexes / Q000737:chemistry / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods; D033363:Neutron Diffraction; D010316:Particle Size; D013499:Surface Properties
chemical_list: D004337:Drug Carriers; D046911:Macromolecular Substances; D046912:Multiprotein Complexes; D005903:Gliadin