[Establishment of RP-HPLC fingerprint for quality assessment of Cortex Periplocae].
OBJECTIVE
To establish the RP-HPLC fingerprint analysis for the quality control of Cortex Periplocae.


METHODS
HPLC fingerprint and analysis method of Cortex Periplocae were studied. Kromasil C18 column (250 mm x 4.6 mm, 5 microm) was used, with mixture of acetonitrile and 0.025% phosphate solution as mobile phase in a gradient mode. The flow rate was 1.0 ml/min. The wavelength of measurement was 220 nm. 15 Batches of Cortex Periplocae were determined.


RESULTS
The 15 samples were classified as 4 clusters by cluster analysis and the 6 superior in producing area samples were confirmed to establish the mutual model. The samples' quality was assessed by "Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004".


CONCLUSIONS
The method is simple and reliable. It can be used to evaluate and control the quality of Cortex Periplocae conveniently.
Li
Dan-yi
DY
School of Pharmacy, Shenyang Pharmaceutical University, China.
Wang
Ting-ting
TT
Chen
Xiao-hui
XH
Bi
Kai-shun
KS
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D031109:Periploca / Q000737:chemistry* / Q000145:classification; D024301:Plant Bark / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal