title: The influence of estradiol and progesterone and melatonin supplementation on TNF-alpha levels in ovariectomized and pinealectomized rats.
abstract: The aim of this study was to investigate the effect of estradiol and progesterone and melatonin supplementation on TNF-alpha levels in ovariectomized and pinealectomized rats. The study was carried out on 42 adult, Spraque-Dawley strain female rats aged 6 months and weighing 200-250 grams. The rats were divided into 6 groups, each group contained 7 rats. Group 1: Sham-ovariectomized (Sham-Ovx), Group 2: Ovariectomized (Ovx), Group 3: Ovx and estradiol (E) and progesterone (P) supplemented (Ovx+E-P) group, Group 4: Ovx+E-P+Melatonin (M) supplemented group, Group 5: Ovx Pinealectomized (Pnx) group, Group 6: Ovx - Pnx+E-P supplemented group. Serum TNF-alpha levels were determined after 4 weeks application period. Group 6 (Ovx-Pnx+E-P) has the highest serum TNF-alpha compared with other groups while group 2 (ovariectomized), has the lowest levels (P<0.001). Group 5 was higher than groups 1, 2, 3 and 4 (P <0.001). The results of the study show that ovariectomy reduces the serum level of TNF-alpha, but estradiol and progesterone application prevents this reduction in ovariectomized rats. However, pinealectomy intensifies the increases in TNF-alpha levels in ovariectomized and estradiol and progesterone supplemented rats, whereas melatonin reduces TNF-alpha levels in ovariectomized rats.
authors.affiliation: Department of Biochemistry, Meram Medical School, Selcuk University, Konya, Turkey.
authors.forename: Esma
authors.initials: E
authors.lastname: Oztekin
authors.forename: R
authors.initials: R
authors.lastname: Mogulkoc
authors.forename: A K
authors.initials: AK
authors.lastname: Baltaci
authors.forename: A M
authors.initials: AM
authors.lastname: Tiftik
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006728:Hormones / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology*; D008954:Models, Biological; D010053:Ovary / Q000502:physiology* / Q000601:surgery; D010870:Pineal Gland / Q000502:physiology* / Q000601:surgery; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D006728:Hormones; D014409:Tumor Necrosis Factor-alpha; D011374:Progesterone; D004958:Estradiol; D008550:Melatonin