[The effect of peripheral blood lymphocyte cell cultured and induced in vitro on the facial nerve regeneration].
OBJECTIVE
To explore the effect of peripheral blood lymphocyte cells cultured in vitro on the facial nerve repair and regeneration.


METHODS
Facial nerves branch-ramus buccolabialis of 20 Wistar male rats were dissected and sutured immediately. Then they were divided into two groups randomly as lymphocytes transfusion group and control group (no cell transfusion). Each group was divided into two sub-groups as 2 w and 8 w. The action potential conduct velocity of the facial nerve was measured and the facial neuron number with HRP retrograde tracing method was counted.


RESULTS
In 8 weeks the action potential conduct velocity of the lymphocytes transfusion group was (0.64 +/- 0.07) m/s, which was significantly faster than that of the control group (0.56 +/- 0.07) m/s (P < 0.05). There was no difference of the facial neuron number among two sub-groups and the control group.


CONCLUSIONS
When purified and amplified in vitro and auto-transfused around the injuries nerve, the peripheral blood lymphocyte cells may have some positive effects on facial nerve repair and regeneration.
Wang
Lifeng
L
Department of Physiology, Pre-clinical College, Xinjiang Medical University, Urumchi, 830054, China.
Ge
Pingjiang
P
Liu
Yanhui
Y
Yu
Xiyong
X
Gao
Zhiqiang
Z
D000818:Animals; D002478:Cells, Cultured; D005154:Facial Nerve / Q000473:pathology / Q000503:physiopathology*; D020220:Facial Nerve Injuries / Q000628:therapy*; D018655:Lymphocyte Count; D017710:Lymphocyte Transfusion; D008214:Lymphocytes; D008297:Male; D009416:Nerve Regeneration; D051381:Rats; D017208:Rats, Wistar