[Determination of saponin I in extracts of Allium macrostemnon].
OBJECTIVE
To develop an HPLC method for determination of saponin I in bulb extracts of Allium macrostemon.


METHODS
The extract was refluxed by methanol and then filtrated. The filtrate was concentrated and the bioactive saponin was measured by HPLC-RID.


RESULTS
The calibration curve was in good linearity over the range of 5-100 microg with the regression equation of Y= 9.35 x 10(4) X - 2.78 x 10(4) (r = 0.9998) and the average recovery was 101.1% (n = 5, RSD 1.6%).


CONCLUSIONS
The method is simple, quick and reproducible and can be applied in the quality control of A. macrostemon.
Chen
Hai-feng
HF
Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, China.
Wang
Nai-li
NL
Dai
Yi
Y
Yao
Xin-sheng
XS
D000490:Allium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D013261:Sterols / Q000032:analysis*
D010936:Plant Extracts; D012503:Saponins; D013261:Sterols