Growth inhibition and apoptosis by (-)-epicatechin gallate are mediated by cyclin D1 suppression in head and neck squamous carcinoma cells.
In recent studies, green tea components have been shown to induce cell growth arrest and apoptosis in head and neck squamous cell carcinoma (HNSCC) cells. In this report, we have investigated the effects of epicatechin gallate (ECG), one of the catechins in green tea, on anti-cancer activity in vitro. We found that cyclin D1 was highly expressed in HNSCC cells, and ECG suppressed 90% of cyclin D1 expression in SCC7 cells. We have also evaluated the effect of ECG on cell growth and apoptosis, showing that ECG (50 microM) exhibited a significant inhibition (50%) on the growth of SCC7 cells via G1 cell cycle arrest. ECG suppressed cyclin D1 in SCC7 cells in a dose- and time-dependent manner, and the suppression of the beta-catenin pathway by ECG is one of the mechanism to facilitate ECG-induced cell growth arrest. These results suggest that ECG has a potential usage as a chemopreventive agent in HNSCC.
Lim
Young Chang
YC
Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996-4542, United States.
Lee
Seong-Ho
SH
Song
Mee Hyun
MH
Yamaguchi
Kiyoshi
K
Yoon
Joo-Heon
JH
Choi
Eun Chang
EC
Baek
Seung Joon
SJ
D000970:Antineoplastic Agents / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000378:metabolism*; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology; D005434:Flow Cytometry; D055436:Growth Differentiation Factor 15; D006131:Growth Inhibitors / Q000627:therapeutic use*; D006258:Head and Neck Neoplasms / Q000188:drug therapy / Q000473:pathology*; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology; D059808:Polyphenols
D000970:Antineoplastic Agents; D016207:Cytokines; D005419:Flavonoids; C526140:GDF15 protein, human; D055436:Growth Differentiation Factor 15; D006131:Growth Inhibitors; D009363:Neoplasm Proteins; D010636:Phenols; D059808:Polyphenols; D019938:Cyclin D1; D002392:Catechin; C062669:epicatechin gallate; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2