[Cannabinoid receptor 1 expression and pathological changes in rat hippocampus after deprivation of rapid eye movement sleep].
To observe the expression of cannabinoid receptor 1 (CB1R) mRNA and pathological changes in rat hippocampus after deprivation of rapid eye movement (REM) sleep.



Totally 42 Sprague-Dawley male rats were randomly divided into cage control (CC), tank control (TC) and the sleep deprivation groups (SD). The SD and TC rats were sacrificed at the end of 1 d, 3 d and 5 d sleep deprivation periods, respectively. The modified multiple platform methods were established for the REM sleep deprivation. CB1R mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR). The hippocampus sections of different stages were observed with electron microscope.



In SD 1 d group, the expression of CB1R mRNA was significantly increased compared with the CC, TC, SD 3 d and SD 5 d groups (P <0.05) while in SD 3 d group it was reduced. The expression of CB1R mRNA of SD 5 d group was significantly higher than that of the SD 3 d group (P <0.05). Neuron apoptosis was found in SD 3 d and SD 5 d groups.



Sleep deprivation can cause brain injury with the changes of CB1R mRNA expression.
Jiang
Pei-fang
PF
The Affiliated Children Hospital, College of Medicine, Zhejianng University, Hangzhou 310003, China. jpf6688@163.com
Zhu
Tao
T
Xia
Zhe-zhi
ZZ
D000818:Animals; D006624:Hippocampus / Q000378:metabolism* / Q000648:ultrastructure*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D043884:Receptor, Cannabinoid, CB1 / Q000096:biosynthesis* / Q000235:genetics; D012892:Sleep Deprivation / Q000378:metabolism / Q000473:pathology; D012895:Sleep, REM
D012333:RNA, Messenger; D043884:Receptor, Cannabinoid, CB1