Characterization of the androgen receptor in a benign prostate tissue-derived human prostate epithelial cell line: RC-165N/human telomerase reverse transcriptase.
The majority of prostate epithelial cell lines stably expressing wild-type (wt) or mutant (mt) androgen receptor (AR) are derived from metastatic prostate cancers. Therefore, the wt AR-expressing RC-165N/human telomerase reverse transcriptase (hTERT) cell line derived from the benign prostate tissue of an African-American patient provides a unique opportunity to assess the functional status of AR in a cellular context not studied before. Although androgen-induced expression of known androgen responsive genes such as PMEPA1, and NDRG1 was observed in RC-165N/hTERT, this cell line expresses prostate-specific antigen (PSA) at significantly lower levels. Chromatin immunoprecipitation assay revealed androgen-dependent binding of AR to androgen response elements of PSA, PMEPA1 and NDRG1 genes. Similarities, as well as differences were noted in the expression of androgen responsive genes between RC-165N/hTERT and LNCaP cells. Comprehensive evaluations of AR functions in RC-165N/hTERT cells suggest that whereas some features of known AR functions are maintained in this benign prostatic tissue-derived cell line, other AR functions are not retained. Objective evaluations of similar cell lines will lead to the understanding of AR functions in prostate growth and differentiation.
Kim
K-H
KH
Department of Surgery, Center for Prostate Disease Research, Uniformed Services University, Bethesda, MD 20852, USA.
Dobi
A
A
Shaheduzzaman
S
S
Gao
C L
CL
Masuda
K
K
Li
H
H
Drukier
A
A
Gu
Y
Y
Srikantan
V
V
Rhim
J S
JS
Srivastava
S
S
D002461:Cell Line, Transformed; D016000:Cluster Analysis; D013196:Dihydrotestosterone / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D017430:Prostate-Specific Antigen / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011944:Receptors, Androgen / Q000378:metabolism* / Q000502:physiology; D020218:Response Elements; D019098:Telomerase / Q000235:genetics*
D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D019098:Telomerase; D017430:Prostate-Specific Antigen