[Isolation and study of active ATP-dependent phosphofructokinase from apple fruits Pyrus domestica Borkh].
The activity of ATP-dependent phosphofructokinase (PFK) from subepidermal tissue of apple fruits was studied. The enzyme extracted under optimal conditions was stable for 14 h at room temperature. The enzyme was partially purified by ammonium sulfate fractionation and dialysis. PFK from apple fruits was found to be ATP-, UTP-, and CTP-specific. It was inhibited by PEP, Gly-2-P, Gly-1,3-DP, and ADP. The effect of the listed inhibitors was diminished by the presence of phosphate. The activity of PFK was stimulated by magnesium cations. The activity of the enzyme in fruits of an Antonovka cultivar was higher than in the Simirenko rennet cultivar by a factor of 1.3.
Giul'akhmetov
S G
SG
Omarov
Ia A
IaA
Mamedov
Z M
ZM
Kuliev
A A
AA
D004791:Enzyme Inhibitors / Q000737:chemistry; D005638:Fruit / Q000201:enzymology*; D025221:Phosphofructokinases / Q000737:chemistry / Q000302:isolation & purification*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D031989:Pyrus / Q000737:chemistry / Q000201:enzymology*; D012265:Ribonucleotides / Q000737:chemistry; D013045:Species Specificity
D004791:Enzyme Inhibitors; D010940:Plant Proteins; D012265:Ribonucleotides; D025221:Phosphofructokinases