A functional FERM domain binding motif in neurofascin.
The L1 family of transmembrane cell adhesion receptors are involved in the development of the nervous system and consist of L1, neuron-glial-related cell adhesion molecule and neurofascin. All three receptors have a short cytoplasmic tail which is known to bind to the cytoskeletal associated protein ankyrin. Ezrin is a cytoplasmic binding protein known to link plasma membrane proteins to the cytoskeleton and has been shown to be a binding partner for L1. Here we show that neurofascin can also interact directly with ezrin. However, the mechanism of interaction of L1 and neurofascin with ezrin is by different mechanisms. We also show that the neurofascin isoform, Nfasc155, co-localizes with ezrin in transfected HEK293 cells but also in interdigitating Schwann cells at the node of Ranvier.
Gunn-Moore
Frank J
FJ
Bute Medical Building, School of Biology, University of St. Andrews, St. Andrews, KY16 9TS, UK. fjg1@st-and.ac.uk
Hill
Maria
M
Davey
Fleur
F
Herron
Lissa R
LR
Tait
Steven
S
Sherman
Diane
D
Brophy
Peter J
PJ
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western / Q000379:methods; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000378:metabolism*; D002461:Cell Line, Transformed; D003593:Cytoplasm / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D039841:Leukocyte L1 Antigen Complex; D009414:Nerve Growth Factors / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011901:Ranvier's Nodes / Q000378:metabolism; D051381:Rats; D014162:Transfection / Q000379:methods; D020798:Two-Hybrid System Techniques; D000099149:Ezrin
D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D039841:Leukocyte L1 Antigen Complex; D009414:Nerve Growth Factors; D000099149:Ezrin; C054305:NFASC protein, human