Physical activity modulates nerve plasticity and stimulates repair after Achilles tendon rupture.
In a rat model of tendon rupture using semiquantitative methodology, healing was assessed according to the diameter of newly organized collagen and the occurrence of the sensory neuropeptides (SP, CGRP) in relation to different levels of physical activity. Normally, innervation of the Achilles tendon is confined to the paratenon. After rupture new nerve fibers grow into the tendon proper, but disappear after healing. In a first experiment to establish peak tissue and nerve regeneration after rupture, tendon tissues from freely moving rats were collected consecutively over 16 weeks. A peak increase in organized collagen and nerve ingrowth was observed between week 2 to 4 post rupture. Therefore, in a second experiment week 4 was chosen to assess the effect of physical activity on tendon healing in three groups of rats, that is, wheel running, plaster treated, and freely moving (controls). In the wheel-running group, the diameter of newly organized collagen was 94% ( p = 0.001) greater than that in the plaster-treated group and 48% ( p = 0.02) greater than that in the controls. Inversely, the neuronal occurrence of CGRP in the tendon proper was 57% ( p = 0.02) lower in the wheel-running group than that in the plaster-treated group and 53% ( p = 0.02) lower than that in the controls, suggesting an earlier neuronal in-growth and disappearance in the more active group. Physical activity speeds up tendon healing, which may prove to be linked to accelerated neuronal plasticity.
Bring
Daniel K-I
DK
Section of Orthopaedics and Sports Medicine, Department of Molecular Medicine and Surgery, Karolinska Institutet, Research Center M3:00, Karolinska Hospital, S-171 76 Stockholm, Sweden. Daniel.Bring@ki.se
Kreicbergs
Andris
A
Renstrom
Per A F H
PA
Ackermann
Paul W
PW
D000125:Achilles Tendon / Q000293:injuries* / Q000294:innervation / Q000503:physiopathology*; D000818:Animals; D003094:Collagen / Q000378:metabolism / Q000648:ultrastructure; D004195:Disease Models, Animal; D008297:Male; D009412:Nerve Fibers / Q000473:pathology; D009473:Neuronal Plasticity / Q000502:physiology*; D009479:Neuropeptides / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012422:Rupture, Spontaneous / Q000503:physiopathology; D013708:Tendon Injuries / Q000503:physiopathology* / Q000534:rehabilitation; D014945:Wound Healing / Q000502:physiology
D009479:Neuropeptides; D003094:Collagen