Performance characteristics of six IMMULITE 2000 TORCH assays.
TORCH is an acronym for Toxoplasma gondii (Toxo), other microorganisms (eg, syphilis), rubella virus (RV), cytomegalovirus (CMV), and herpes simplex virus (HSV) that are associated with congenital abnormalities from maternal infection. We evaluated linearity, imprecision, and comparison with commercially available methods of the IMMULITE 2000 (Diagnostic Products, Los Angeles, CA) Toxo IgG, Toxo IgM, RV IgG, RV IgM, CMV IgG, and HSV IgG assays. Linearity and imprecision results were acceptable. The IMMULITE 2000 assays show good concordance with other commercially available methods except for Toxo IgM and RV IgM. Toxo IgM showed better concordance with a consensus of 3 of 4 (Access, Beckman Coulter, Fullerton, CA; IMMULITE 2000; Platelia, Bio-Rad Laboratories Diagnostics Group, Redmond, WA; and Vidas, bioMerieux, Hazelwood, MO) assays than with Access alone. The RV IgM assay showed better concordance with the Zeus method than with the Diamedix method (Diamedix, Miami, FL). The IMMULITE 2000 TORCH assays studied show acceptable performance and are suitable for routine clinical use. Some commercial assays for Toxo IgM and RV IgM show rather poor concordance.
Owen
William E
WE
ARUP Institute for Clinical and Experimental Pathology, University of Utah, Health Sciences Center, Salt Lake City 84108, USA.
Martins
Thomas B
TB
Litwin
Christine M
CM
Roberts
William L
WL
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000914:Antibodies, Viral / Q000032:analysis; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012698:Serologic Tests / Q000379:methods*; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis* / Q000276:immunology
D000913:Antibodies, Protozoan; D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic