Expression of mRNA for several enzymes of xenobiotic detoxification in normal and spontaneously transformed mesothelial cells and mesothelioma cells of rats.
Expression of mRNA for the mdr1 gene, cytochrome P450 isoforms 1A1 and 1B1, Ah receptor, and ARNT protein regulating the concentration of cytochrome P450 mRNA was compared in normal and spontaneously transformed mesothelial cells and mesothelioma cells from rats. Expression of cytochrome P450 1A1 and 1B1 mRNA decreased in transformed mesothelial and mesothelioma cells compared to normal mesothelial cells. mRNA for the mdr1 gene was undetected in normal mesothelial cells. Expression of mRNA for the Ah receptor and ARNT protein did not differ in cultured cells.
Kobliakov
V A
VA
Institute of Carcinogenesis, Russian Academy of Medical Sciences, Moscow. vakob@crc.umos.ru
Vaiman
A V
AV
Pylev
L N
LN
Vasil'eva
L A
LA
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D065633:Cytochrome P-450 CYP1B1; D017931:DNA Primers; D004848:Epithelium / Q000201:enzymology* / Q000473:pathology; D008658:Inactivation, Metabolic; D008654:Mesothelioma / Q000201:enzymology* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
D017931:DNA Primers; D012333:RNA, Messenger; D001189:Aryl Hydrocarbon Hydroxylases; C585499:Cyp1b1 protein, rat; D019363:Cytochrome P-450 CYP1A1; D065633:Cytochrome P-450 CYP1B1