title: Multiple vaccine and pyridostigmine bromide interactions in the common marmoset Callithrix jacchus: immunological and endocrinological effects.
abstract: Following active service during the 1990/1991 Gulf Conflict, a number of UK and US veterans presented with a diverse range of symptoms, collectively known as Gulf Veterans Illnesses (GVI). The administration of vaccines and/or the pretreatment against possible nerve agent poisoning, pyridostigmine bromide (PB), given to Armed Forces personnel during the Gulf Conflict has been implicated as a possible factor in the aetiology of these illnesses. The possibility that adverse health effects may result from the administration of these vaccines (anthrax, pertussis, plague, yellow fever, polio, typhoid, tetanus, hepatitis B, meningococcal meningitis and cholera) and/or PB, have been investigated over an eighteen month period, in a non-human primate model, the common marmoset. This study reports immunological indices, including leukocyte phenotypes, intracellular cytokines IFN-gamma and IL-4 and antibody responses against vaccine antigens. Using human isotyping reagents previously shown to cross react with marmoset immunoglobulins (ibid) it was shown that marmosets responded strongly against anthrax PA and pertussis and weakly against killed whole cell plague, cholera and typhoid. At the end of the study the immune response to a previously unseen T-cell dependent antigen, keyhole limpet haemocyanin (KLH), was examined in order to determine whether immune function had been compromised by the compounds administered. Statistically equivalent, robust antibody responses were measured against KLH in all treatment groups indicating that the immune system had not been compromised by any of the treatments. In addition, urinary cortisol was measured at key points throughout the study as an index of physiological stress which may have been induced by the treatments. There were no effects of treatment on urinary cortisol secretion. With respect to the other immunological indices measured, there were no statistical differences between the treatment groups during the period of the study.
authors.affiliation: Biomedical Sciences Department, Defence Science and Technology Laboratory, Porton Down, Salisbury, UK.
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Hornby
authors.forename: Peter C
authors.initials: PC
authors.lastname: Pearce
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Bowditch
authors.forename: Leah
authors.initials: L
authors.lastname: Scott
authors.forename: Gareth D
authors.initials: GD
authors.lastname: Griffiths
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001428:Bacterial Vaccines / Q000494:pharmacology; D002144:Callithrix / Q000276:immunology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004347:Drug Interactions; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D006854:Hydrocortisone / Q000652:urine; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology; D018923:Persian Gulf Syndrome; D011729:Pyridostigmine Bromide / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014765:Viral Vaccines / Q000494:pharmacology
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D002800:Cholinesterase Inhibitors; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014765:Viral Vaccines; D015847:Interleukin-4; D007371:Interferon-gamma; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D011729:Pyridostigmine Bromide; D006854:Hydrocortisone