Phosphoinositide synthesis and degradation in isolated rat liver peroxisomes.
Analyzing peroxisomal phosphoinositide (PId(#)) synthesis in highly purified rat liver peroxisomes we found synthesis of phosphatidylinositol 4-phosphate (PtdIns4P), PtdIns(4,5)P(2) and PtdIns(3,5)P(2). PtdIns3P was hardly detected in vitro, however, was observed in vivo after [(32)P]-phosphate labeling of primary rat hepatocytes. In comparison with other subcellular organelles peroxisomes revealed a unique PId pattern suggesting peroxisomal specificity of the observed synthesis. Use of phosphatase inhibitors enhanced the amount of PtdIns4P. The results obtained provide evidence that isolated rat liver peroxisomes synthesize PIds and suggest the association of PId 4-kinase and PId 5-kinase and PId 4-phosphatase activities with the peroxisomal membrane.
Jeynov
Boyan
B
Biochemie-Zentrum der Universität Heidelberg (BZH), Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
Lay
Dorothee
D
Schmidt
Frank
F
Tahirovic
Sabina
S
Just
Wilhelm W
WW
D019870:1-Phosphatidylinositol 4-Kinase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism*; D066298:In Vitro Techniques; D020675:Peroxisomes / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000096:biosynthesis / Q000378:metabolism; D010716:Phosphatidylinositols / Q000096:biosynthesis* / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism
D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; C037178:phosphatidylinositol 4-phosphate; D019870:1-Phosphatidylinositol 4-Kinase; D010744:Phosphoric Monoester Hydrolases