Comparison of the binding of anti-tubulin antibody and the fluorescent taxol derivative Flutax-1 to the microtubular system of Tetrahymena.
Using confocal microscopic analysis, FITC-labelled anti-alpha-tubulin antibody and the fluorescent taxol derivative Flutax-1 in fixed and living Tetrahymena pyriformis GL, longitudinal microtubules, oral and somatic cilia, deep fibers, and contractile vacuole pores were equally labeled. While the antibody stained transversal microtubules, these were not labeled by Flutax-1. At the same time, oral cilia were more intensely stained by Flutax-1, than by the antibody. There were no differences in the staining of fixed preparations and living cells. The observations suggest (i) the difference between the MAPs of longitudinal and transversal microtubules which allow or inhibit the binding of the indicator molecules, and (ii) the different functions of these two types of microtubules.
Kovács
P
P
Department of Genetics, Cell and Immunobiology, Semmelweis University, Nagyvárad tér 4, Budapest, Hungary. kovpet@dgci.sote.hu
Csaba
G
G
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D002923:Cilia / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000494:pharmacology; D018613:Microscopy, Confocal; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D011485:Protein Binding; D043823:Taxoids / Q000737:chemistry* / Q000378:metabolism; D013769:Tetrahymena pyriformis / Q000378:metabolism; D014404:Tubulin / Q000737:chemistry* / Q000276:immunology
C116523:7-O-(N-(4'-fluoresceincarbonyl)alanyl)taxol; D000906:Antibodies; D005456:Fluorescent Dyes; D008869:Microtubule-Associated Proteins; D043823:Taxoids; D014404:Tubulin; D016650:Fluorescein-5-isothiocyanate