Directed evolution of hybrid enzymes: Evolving enantioselectivity of an achiral Rh-complex anchored to a protein.
The concept of utilizing the methods of directed evolution for tuning the enantioselectivity of synthetic achiral metal-ligand centers anchored to proteins has been implemented experimentally for the first time.
Reetz
Manfred T
MT
Max-Planck-Institut für Kohlenforschung, D-45470 Mülheim/Ruhr, Germany. reetz@mpi-muelheim.mpg.de
Peyralans
Jérôme J-P
JJ
Maichele
Andrea
A
Fu
Yu
Y
Maywald
Matthias
M
D000179:Acrylates / Q000737:chemistry; D002384:Catalysis; D019020:Directed Molecular Evolution; D004798:Enzymes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015723:Gene Library; D006860:Hydrogen Bonding; D008958:Models, Molecular; D016296:Mutagenesis; D012238:Rhodium / Q000737:chemistry*; D013237:Stereoisomerism
D000179:Acrylates; D004798:Enzymes; D012238:Rhodium; C036658:acrylic acid