title: Vasorelaxant effect of the rootbark extract of Paeonia moutan on isolated rat thoracic aorta.
abstract: The vascular relaxant effect of the rootbark extract of Paeonia moutan was evaluated in isolated rat thoracic aorta. The methanolic extract of the rootbark showed a vasorelaxant activity in rat aortic preparations precontracted with 0.3 microM phenylephrine (IC50 value: 16.8 microg/mL). The activity-guided fractionation of the extract led to the isolation of five active principles such as paeoniflorin (1), paeonidanin (2), methylpaeoniflorin (4), tetragalloylglucose (5) and pentagalloylglucose (6), and these active ingredients potently relaxed phenylephrine-induced contraction of rat aortic preparations in a concentration-dependent manner (IC50 values: 19.4, 7.9, 10.1, 5.1 and 3.6 microM, respectively). These results suggest that pinane glycosides and galloylglucoses might be the components responsible for the vasorelaxant properties of the rootbark extract of P. moutan, and their vasorelaxant effects may be mediated through increases in the release of nitric oxide from endothelial cells.
authors.affiliation: Korea Research Institute of Chemical Technology, Daejeon, Korea.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Yoo
authors.forename: Byung Ho
authors.initials: BH
authors.lastname: Lee
authors.forename: Yeon Hee
authors.initials: YH
authors.lastname: Choi
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Jee Hee
authors.initials: JH
authors.lastname: Seo
authors.forename: Kwang-Seok
authors.initials: KS
authors.lastname: Oh
authors.forename: Hyun-Na
authors.initials: HN
authors.lastname: Koo
authors.forename: Ho Won
authors.initials: HW
authors.lastname: Seo
authors.forename: Gyu Hwan
authors.initials: GH
authors.lastname: Yon
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kwon
authors.forename: Young Sup
authors.initials: YS
authors.lastname: Kim
authors.forename: Shi Yong
authors.initials: SY
authors.lastname: Ryu
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D029596:Paeonia; D010656:Phenylephrine; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000187:drug effects*; D014665:Vasodilator Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D010936:Plant Extracts; D014665:Vasodilator Agents; D010656:Phenylephrine