[Determination of scopolamine and atropine in Flos Daturae by RP-HPLC].
OBJECTIVE
To develop a quantitative analytical procedure of scopolamine and atropine in Flos Daturae using RP-HPLC.


METHODS
The two alkaloids were separated on a Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm) with a mobile phase of 0.02 mol x L(-1) sodium acetate buffer (containing 0.02% triethanolamine and the pH was adjusted to 6.0 with acetic acid)-methanol (60:40) and a detection wavelength of 215 nm. The flow rate was 1.0 mL x min(-1) and the column temperature was maintained at room temperature.


RESULTS
The mean recovery was (99.6 +/- 1.8)% for scopolamine and (100.4 +/- 1.5)% for atropine.


CONCLUSIONS
This method was simple, accurate and sensitive.
Hou
Shi-guo
SG
Department of Chemistry, Capital Normal University, Beijing, China.
Gu
Xue-xin
XX
Wang
Shu-yan
SY
Li
Hong-xia
HX
D001285:Atropine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032302:Datura / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012601:Scopolamine / Q000032:analysis*
D001285:Atropine; D012601:Scopolamine