Determination of bisphenol A in wine by sol-gel immunoaffinity chromatography, HPLC and fluorescence detection.
The paper presents a highly selective analytical method for the determination of traces of bisphenol A (BPA) in wine and the results of a survey 59 wine samples sourced from vats (steel, wood and plastic), glass bottles and Tetra briks. The procedure consists of sample clean-up by sol-gel immunoaffinity chromatography followed by determination of BPA by high-performance liquid chromatography and fluorescence detection. The method has a limit of detection (LOD) (S/N = 3) of 0.1 ng ml-1 and a limit of quantitation (LOQ) (S/N = 6) of 0.2 ng ml-1. In 13 of 59 wine samples, the BPA concentration was below the LOQ. The mean and median for all wine samples with BPA concentrations above the LOQ were 0.58 and 0.40 ng ml-1, respectively. These values - the first set of data on BPA in wine - are far lower than previously published BPA levels derived from migration experiments using wine simulants. Experiments carried out by submerging plastic stoppers in ethanol-water (11 : 89, v/v) up to 11 weeks indicated that detectable amounts of BPA can be leached from some stoppers.
Brenn-Struckhofova
Z
Z
Department of Analytical and Food Chemistry, University of Vienna, Währinger Strasse 38, A-1090 Vienna, Austria.
Cichna-Markl
M
M
D001559:Benzhydryl Compounds; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005782:Gels; D010636:Phenols / Q000032:analysis*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D014920:Wine / Q000032:analysis*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D005782:Gels; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds