title: Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1.
abstract: A newly isolated strain of Kluyveromyces marxianus YS-1 was used for the production of extra cellular inulinase in a medium containing inulin, meat extract, CaCl2 and sodium dodecyl sulphate (SDS). Fermentation medium pH 6.5, cultivation temperature 30 degrees C and 5% (v/v) inoculum of 12 h-old culture were optimal for enzyme production (30.8 IU/ml) with a fermentation time of 72 h at shake flask level. Raw inulin (2%, w/v) extracted from dahlia tubers by processing at 15 kg/cm2 for 10 min was optimum for bioreactor studies. Maximum enzyme production (55.4 IU/ml) was obtained at an agitation rate of 200 rpm and aeration of 0.75 vvm in a stirred tank reactor with a fermentation time of 60 h.
authors.affiliation: Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala 147 002, India. rsingh11@lycos.com
authors.forename: R S
authors.initials: RS
authors.lastname: Singh
authors.forename: Balwinder Singh
authors.initials: BS
authors.lastname: Sooch
authors.forename: Munish
authors.initials: M
authors.lastname: Puri
mesh_terms: D019149:Bioreactors; D003470:Culture Media; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000302:isolation & purification; D007716:Kluyveromyces / Q000201:enzymology*; D009584:Nitrogen / Q000378:metabolism; D013501:Surface-Active Agents; D014131:Trace Elements / Q000378:metabolism
chemical_list: D003470:Culture Media; D013501:Surface-Active Agents; D014131:Trace Elements; D006026:Glycoside Hydrolases; C023086:inulinase; D009584:Nitrogen