Preparation of monoclonal antibody bank against whole water-soluble proteins from rapid-growing bamboo shoots.
An antibody bank against the whole proteins in a proteome is a useful tool for biological research. Using the standard cell fusion method, and a modified screening protocol, we produced an mAb bank against the total water-soluble proteins extracted from the rapid-growing green bamboo shoots. An improved two-stage strategy was employed to enrich those poor immunogenic or lower expressed proteins. Totally, we obtained a bank of 192 mAb which were identified as distinctive to each other by 2-DE and immunostaining.
Wu
Yu-Jen
YJ
Department of Biochemical Science and Technology, Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan.
Chen
Han-Min
HM
Wu
Tai-Tse
TT
Wu
Jiann-Shing
JS
Chu
Rea-Min
RM
Juang
Rong-Huay
RH
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D031723:Bambusa / Q000276:immunology*; D004353:Drug Evaluation, Preclinical; D007123:Immunoelectrophoresis, Two-Dimensional; D011506:Proteins / Q000276:immunology*; D020543:Proteome / Q000276:immunology / Q000378:metabolism*; D012995:Solubility; D014867:Water
D000911:Antibodies, Monoclonal; D011506:Proteins; D020543:Proteome; D014867:Water