Purification and biological effects of Araucaria angustifolia (Araucariaceae) seed lectin.
This paper describes the purification and characterization of a new N-acetyl-d-glucosamine-specific lectin from Araucaria angustifolia (AaL) seeds (Araucariaceae) and its anti-inflammatory and antibacterial activities. AaL was purified using a combination of affinity chromatography on a chitin column and ion exchange chromatography on Sephacel-DEAE. The pure protein has 8.0kDa (SDS-PAGE) and specifically agglutinates rabbit erythrocytes, effect that was independent of the presence of divalent cations and was inhibited after incubation with glucose and N-acetyl-d-glucosamine. AaL showed antibacterial activity against Gram-negative and Gram-positive strains, shown by scanning electron microscopy. AaL, intravenously injected into rats, showed anti-inflammatory effect, via carbohydrate site interaction, in the models of paw edema and peritonitis. This lectin can be used as a tool for studying bacterial infections and inflammatory processes.
Santi-Gadelha
Tatiane
T
Departamento de Biologia Molecular, Universidade Federal da Paraíba, Campus I, João Pessoa, Paraíba, Brazil.
de Almeida Gadelha
Carlos Alberto
CA
Aragão
Karoline Sabóia
KS
de Oliveira
Cecília Carvalho
CC
Lima Mota
Mário Rogério
MR
Gomes
Raphaela Cardoso
RC
de Freitas Pires
Alana
A
Toyama
Marcos Hikari
MH
de Oliveira Toyama
Daniela
D
de Alencar
Nylane Maria Nunes
NM
Criddle
David Neil
DN
Assreuy
Ana Maria Sampaio
AM
Cavada
Benildo Sousa
BS
D000818:Animals; D001419:Bacteria / Q000166:cytology* / Q000187:drug effects*; D032462:Cycadopsida / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000188:drug therapy*; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification; D037121:Plant Lectins / Q000008:administration & dosage* / Q000302:isolation & purification; D051381:Rats; D012639:Seeds / Q000737:chemistry*
D010936:Plant Extracts; D037121:Plant Lectins