Prevalence of genes encoding exfoliative toxins among Staphylococcus hyicus isolated in Russia and Germany.
In the present study, previously characterized Staphylococcus hyicus isolated in Russia (n=23) and Germany (n=17) were investigated for the prevalence of the exfoliative toxin encoding genes exhA, exhB, exhC and exhD by multiplex PCR resulting in the detection of exhD positive strains among the S. hyicus isolated from pigs with exudative epidermitis in Russia and the detection of exhC and exhD for one and two strains isolated from exudative epidermitis in Germany respectively. The toxin gene negative strains were generally isolated from apparently healthy pigs, from other animals and from specimens where the relation between the isolation of S. hyicus and the clinical symptoms remained unclear. Partial sequencing of the toxin genes of selected exhC and exhD positive strains and comparing the sequencing results with sequences of exhC and exhD reference strains revealed an almost complete identity. The results of the present study were in agreement with the findings of Andresen and Ahrens (J. Appl. Microbiol., 96, 2004, 1265) and Andresen (J. Vet. Rec., 157, 2005, 376) that the presented multiplex PCR could be used to investigate S. hyicus for toxinogenic potential and that there is an association between the presence of toxin genes in S. hyicus strains from exudative epidermitis. However, comparable with the S. hyicus strains isolated in Germany which were investigated previously by Andresen (J. Vet. Rec., 157, 2005, 376), exhD seems to predominate in S. hyicus strains from Russia.
Kanbar
T
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Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Frankfurter Str. 107, 35392 Giessen, Germany.
Voytenko
A V
AV
Alber
J
J
Lämmler
C
C
Weiss
R
R
Zschöck
M
M
Shilov
I A
IA
Dmitrenko
O A
OA
Gintsburg
A L
AL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004818:Epidermitis, Exudative, of Swine / Q000378:metabolism / Q000382:microbiology*; D005083:Exfoliatins / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D005858:Germany; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012426:Russia; D016415:Sequence Alignment; D013210:Staphylococcus / Q000235:genetics* / Q000378:metabolism; D013552:Swine
D005083:Exfoliatins