title: Relationship among antimutagenic, antioxidant and enzymatic activities of methanolic extract from common beans (Phaseolus vulgaris L).
abstract: Common beans are rich in phenolic compounds, which can provide health benefits to the consumer. The objective of this work was to study the relationship among antimutagenicity, antioxidant and enzymatic activities of methanolic extract and trolox by principal components multivariate analysis. Antimutagenicity of phenolic compounds present in methanolic extract from the seed coat of common beans (P. vulgaris, Flor de Mayo Bajío cultivar) and trolox against AFB1 mutagenicity were evaluated in the Salmonella typhimurium microsuspension assay. Antioxidant capacity of methanolic extract and trolox were evaluated using beta-carotene and 1,1-diphenyl-2-picryhydrazyl (DPPH) in vitro model assays. Cythrome P450 activity was measured by fluorometric assay. For phenolic extract, trolox and phenolic extract+trolox, the inhibition on AFB1 mutagenicity in tester strain TA100 was 47, 59 and 69%, respectively. While in TA98 was 39, 48 and 68%. The inhibition of phenolic compounds, trolox and phenolic compounds+trolox on cytochrome P450 (CYP450) activity was 48, 59 and 88%, respectively. Correlation analysis showed that phenolic extract and trolox have high antimutagenic and antioxidant activity and also inhibited enzymatic activity. The results suggest that the primary mechanism of action of phenolic compounds in beans against AFB1 mutagenicity may be extra-cellular in the microsuspension assay.
authors.affiliation: PROPAC (Programa de Posgrado en Alimentos del Centro de la República), Research and Graduated Studies in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, Querétaro, Qro., 76010, Mexico.
authors.forename: Anaberta
authors.initials: A
authors.lastname: Cardador-Martínez
authors.forename: Arnulfo
authors.initials: A
authors.lastname: Albores
authors.forename: Moustapha
authors.initials: M
authors.lastname: Bah
authors.forename: Victor
authors.initials: V
authors.lastname: Calderón-Salinas
authors.forename: Eduardo
authors.initials: E
authors.lastname: Castaño-Tostado
authors.forename: Ramón
authors.initials: R
authors.lastname: Guevara-González
authors.forename: Armando
authors.initials: A
authors.lastname: Shimada-Miyasaka
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Loarca-Piña
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D015999:Multivariate Analysis; D027805:Phaseolus / Q000201:enzymology; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D010936:Plant Extracts; D003577:Cytochrome P-450 Enzyme System; D016604:Aflatoxin B1