title: Diet supplemented with citrus unshiu segment membrane suppresses chemically induced colonic preneoplastic lesions and fatty liver in male db/db mice.
abstract: The modulatory effects of dietary citrus unshiu segment membrane (CUSM) on the occurrence of aberrant crypt foci (ACF) and beta-catenin accumulated crypts (BCACs) were determined in male C57BL/KsJ-db/db (db/db) mice initiated with azoxymethane (AOM). Male db/db, db/+ and +/+ mice were given 5 weekly subcutaneous injections of AOM (15 mg/kg body weight), and then they were fed the diet containing 0.02%, 0.1% or 0.5% CUSM for 7 weeks. At Week 12, a significant increase in the numbers of ACF and BCAC was noted in the db/db mice in comparison with the db/+ and +/+ mice. Feeding with CUSM caused reduction in the frequency of ACF in all genotypes of mice and the potency was high in order of the db/db mice, db/+ mice and +/+ mice. The number of BCACs was also reduced by feeding with CUSM, thus resulting in a 28-61% reduction in the db/db mice, possibly due to suppression of cell proliferation activity in the lesions by feeding with CUSM-containing diet. Clinical chemistry revealed a low serum level of triglyceride in mice fed CUSM. In addition, CUSM feeding inhibited fatty metamorphosis and fibrosis in the liver of db/db mice. Our findings show that CUSM in the diet has a chemopreventive ability against the early phase of AOM-induced colon carcinogenesis in the db/db as well as db/+ and +/+ mice, indicating potential use of CUSM in cancer chemoprevention in obese people.
authors.affiliation: Department of Oncologic Pathology, Kanazawa Medical University, Ishikawa, Japan.
authors.forename: Rikako
authors.initials: R
authors.lastname: Suzuki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kohno
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Yasui
authors.forename: Kazuya
authors.initials: K
authors.lastname: Hata
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Sugie
authors.forename: Shingo
authors.initials: S
authors.lastname: Miyamoto
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Sugawara
authors.forename: Takashi
authors.initials: T
authors.lastname: Sumida
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Hirose
authors.forename: Takuji
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D001397:Azoxymethane / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity; D002784:Cholesterol / Q000097:blood; D003106:Colon / Q000737:chemistry / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D004032:Diet; D019587:Dietary Supplements; D005234:Fatty Liver / Q000517:prevention & control*; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D008099:Liver / Q000737:chemistry / Q000473:pathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010936:Plant Extracts / Q000008:administration & dosage*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000517:prevention & control*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D014280:Triglycerides / Q000097:blood
chemical_list: D002273:Carcinogens; D007328:Insulin; D020738:Leptin; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D014280:Triglycerides; D002784:Cholesterol; D001397:Azoxymethane