[Real-time PCR: approaches to data analysis (a review)].
The registration of the accumulation of polymerase chain reaction (PCR) products in the course of amplification (real-time PCR) requires specific equipment, i.e., detecting amplifiers capable of recording the level of fluorescence in the reaction tube during amplicon formation. By the time the reaction is completed, researchers obtain DNA accumulation graphs. The review discusses the most promising algorithms of analysis of real-time PCR curves and possible errors, whether caused by the software used or the operators' mistakes. The data included will assist researchers in understanding the features of the method to obtain more reliable results.
Rebrikov
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Trofimov
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D000465:Algorithms; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards / Q000639:trends; D012015:Reference Standards; D015203:Reproducibility of Results; D012984:Software