title: Microbial biochemistry, physiology, and biotechnology of hyperthermophilic Thermotoga species.
abstract: High-throughput sequencing of microbial genomes has allowed the application of functional genomics methods to species lacking well-developed genetic systems. For the model hyperthermophile Thermotoga maritima, microarrays have been used in comparative genomic hybridization studies to investigate diversity among Thermotoga species. Transcriptional data have assisted in prediction of pathways for carbohydrate utilization, iron-sulfur cluster synthesis and repair, expolysaccharide formation, and quorum sensing. Structural genomics efforts aimed at the T. maritima proteome have yielded hundreds of high-resolution datasets and predicted functions for uncharacterized proteins. The information gained from genomics studies will be particularly useful for developing new biotechnology applications for T. maritima enzymes.
authors.affiliation: Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
authors.forename: Shannon B
authors.initials: SB
authors.lastname: Conners
authors.forename: Emmanuel F
authors.initials: EF
authors.lastname: Mongodin
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Johnson
authors.forename: Clemente I
authors.initials: CI
authors.lastname: Montero
authors.forename: Karen E
authors.initials: KE
authors.lastname: Nelson
authors.forename: Robert M
authors.initials: RM
authors.lastname: Kelly
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D001709:Biotechnology; D050260:Carbohydrate Metabolism; D014644:Genetic Variation; D016680:Genome, Bacterial; D023281:Genomics; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000235:genetics / Q000502:physiology*; D006358:Hot Temperature; D007501:Iron / Q000378:metabolism; D009005:Monosaccharides / Q000096:biosynthesis; D010802:Phylogeny; D011134:Polysaccharides / Q000096:biosynthesis; D015398:Signal Transduction; D013455:Sulfur / Q000378:metabolism; D020124:Thermotoga maritima / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D009005:Monosaccharides; D011134:Polysaccharides; D013455:Sulfur; D007501:Iron