A method of combined single-cell electrophysiology and electroporation.
This paper describes a method of extracellular recording and subsequent electroporation with the same electrode in single retinal ganglion cells in vitro. We demonstrate anatomical identification of neurons whose receptive fields were measured quantitatively. We discuss how this simple method should also be applicable for the delivery of a variety of intracellular agents, including gene delivery, to physiologically characterized neurons, both in vitro and in vivo.
Graham
Lyle J
LJ
Laboratory of Neurophysics and Physiology, CNRS UMR 8119, UFR Biomédicale de l'Université René Descartes, 45 rue des Saint-Pères, 75006 Paris, France. lyle@biomedicale.univ-paris5.fr
Del Abajo
Ricardo
R
Gener
Thomas
T
Fernandez
Eduardo
E
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D018274:Electroporation / Q000379:methods*; D010775:Photic Stimulation / Q000379:methods; D011817:Rabbits; D012160:Retina / Q000166:cytology; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000502:physiology