[Expression of c-fos, OPG, OPGL in rabbit mandibular distraction osteogenesis zone].
To evaluate the possible signal transduction mechanism of the mechanical stress induced by the distraction procedure in osteocytes.



An animal model of mandibular distraction osteogenesis in rabbits was established. The expressions of c-fos, OPG and OPGL were detected by ultrasensitive S-P immunohistochemical method.



At 4 and 8 days after distraction, distraction zone showed strong positive staining of c-fos, which were apparently higher than that in distraction zone of 2, 4 and 6 weeks after consolidation. At 4 and 8 days after distraction and 2 weeks after consolidation, the expression of OPG was strong, and then wore off gradually at 4 and 6 weeks after consolidation. Weak signals of OPGL could be detected at 6 weeks after consolidation only.



c-fos, OPG and OPGL are important regulators in distraction osteogenesis. c-fos is interrelated with the mechanical stress induced by the distraction procedure closely, OPG promotes new bone formation, while OPGL plays a more active role in bone remodeling.
Ge
Wei-li
WL
The Affiliated Stomatological Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China.
Xie
Zhi-jian
ZJ
He
Jian-feng
JF
D000818:Animals; D008334:Mandible / Q000166:cytology / Q000378:metabolism*; D010011:Osteocytes / Q000378:metabolism; D019857:Osteogenesis, Distraction; D053244:Osteoprotegerin / Q000096:biosynthesis* / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D053245:RANK Ligand / Q000096:biosynthesis* / Q000235:genetics; D011817:Rabbits; D011897:Random Allocation
D053244:Osteoprotegerin; D016760:Proto-Oncogene Proteins c-fos; D053245:RANK Ligand