title: Giardia duodenalis: analysis of secreted proteases upon trophozoite-epithelial cell interaction in vitro.
abstract: Protease secretion by Giardia duodenalis trophozoites upon interaction with epithelial cells and its association with the parasite adhesion was studied in co-cultures of parasites with IEC6 epithelial cell monolayers in the presence or absence of protease inhibitors. Proteolytic activity in supernatants from trophozoites was enhanced when they were co-cultured with IEC6 cells. This activity was strongly inhibited by pre-incubation of live trophozoites with E-64 and TPCK and a concomitant inhibition of parasite adhesion to IEC6 cells was observed. These data suggest that trophozoites secrete cysteine-type proteases that play a role in the adhesion of G. duodenalis to epithelial cells.
authors.affiliation: Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social-Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
authors.forename: Guadalupe Beatriz
authors.initials: GB
authors.lastname: Rodríguez-Fuentes
authors.forename: Roberto
authors.initials: R
authors.lastname: Cedillo-Rivera
authors.forename: Rocío
authors.initials: R
authors.lastname: Fonseca-Liñán
authors.forename: Raúl
authors.initials: R
authors.lastname: Argüello-García
authors.forename: Onofre
authors.initials: O
authors.lastname: Muñoz
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Ortega-Pierres
authors.forename: Lilián
authors.initials: L
authors.lastname: Yépez-Mulia
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D002460:Cell Line; D004847:Epithelial Cells / Q000201:enzymology*; D005872:Giardia / Q000166:cytology / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000187:drug effects / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology*
chemical_list: D011480:Protease Inhibitors; D010447:Peptide Hydrolases