[Inhibition effect of active fraction from Actinidia valvata on growth of transplanated mouse tumor cells and preliminary study of its mechanism].
OBJECTIVE
To study the inhibition effect of active fraction from maorenshen (MA, MB) on the growth of transplanted mouse tumor cells H22 and its mechanism.


METHODS
The transplanted mouse tumor model in vivo was used to observe the antitumor activity of MA, MB. The cell cycle distributions were determined by flow cytometry (FCM). The apoptosis in tumor was measured by TdT-mediated biotinyated-dUTP nick end-labeling (TUNEL) method.


RESULTS
MA, MB showed antitumor activitiy on transplanted mouse tumor. They could make cells arrested at G0-G1 phase, decrease cells percentage at S phase, and induce their apoptosis.


CONCLUSIONS
MA, MB from maorenshen have anti-tumor effect on the transplanted mouse tumor, and the mechanism may be through the way of influencing cell cycle and inducing apoptosis.
Zhang
Ya-ni
YN
Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Liu
Ling
L
Ling
Chang-quan
CQ
D029042:Actinidia / Q000737:chemistry; D000818:Animals; D000880:Anthraquinones / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology
D000880:Anthraquinones; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D012503:Saponins