[Protective effect of octreotide on liver warm ischemia reperfusion injury].
OBJECTIVE
To explore the protective effect of octreotide on liver warm ischemia-reperfusion injury and its possible mechanism.


METHODS
Pringle's maneuver liver ischemia-reperfusion models were established. Forty eight male Sprague Daweley rats were randomly divided into a sham operation group (S group, n=16), an ischemia-reperfusion group (I/R group, n=16) and an octreotide preconditioning group (OPC group, n=16). ALT and AST in the serum were measured at 30 min after the ischemia and 120 min after the reperfusion. The histomorphological changes and ultrastructure of hepatocellular were observed by optic and transmission electronic microscope. Hepatic adenine nucleotide levels and energy changes (EC) were determined by high performance liquid chromatography (HPLC).


RESULTS
(1) At 30 min after the ischemia and 120 min after the reperfusion, the levels of ALT and AST in the serum of OPC group was lower than those in I/R group, whereas the levels of ATP and EC in the hepatic tissue were higher than those in the I/R group (P<0.01 or P<0.05). Compared with the I/R group, the injury of hepatocellular histomorphology and ultrastructure in the OPC group was abated. (2) At 30, 60, and 120 min after the reperfusion, the levels of ATP and EC in the OPC groups were higher than those in the I/R group. During the ischemia, the levels of ATP and EC in the OPC group dropped more slowly than those in the I/R group, but ATP and EC in the OPC groups rose more quickly than those in the I/R group during the reperfusion.


CONCLUSIONS
Octreotide precondition can improve the hepatocellular energy reserve, and protect the liver from warm ischemia-reperfusion injury. The protective of octreotide on warm ischemia-reperfusion injury may be related to its influence on endocrine secretion.
Li
Jie-qun
JQ
Organ Transplantation Center, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Qi
Hai-zhi
HZ
He
Zhi-jun
ZJ
Hu
Wei
W
Si
Zhong-zhou
ZZ
Li
Yi-ning
YN
D000818:Animals; D006358:Hot Temperature; D008099:Liver / Q000098:blood supply*; D008297:Male; D015282:Octreotide / Q000494:pharmacology* / Q000627:therapeutic use; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*
D020011:Protective Agents; D015282:Octreotide