[Study on the membrane protein conformational changes and mechanisms of myocardial cell irradiated by pulse microwave].
Micro-Fourier transform infrared spectroscopy (FTIR) technique was applied to study the membrane protein conformational and functional changes of myocardial cell irradiated by pulse microwave. The results show that pulse microwave could influence the membrane protein structure markedly. The stretching vibration of lipid--CH2--, lecithoid C=O, amide I and II region was decreased or displaced. The secondary structures of membrane protein were also changed by irradiation. The percentage of alpha-helix and beta-pleated sheet structure decreased remarkably, and the disordering of secondary membrane proteins increased. All the above changes are correlated with the irradiation dosage. The results indicated that the integrality of myocardial cell membrane was injured by pulse microwave, and the membrane fluidity and stability decreased. Multi-biochemically active structures were damaged. Then all the changes could make a biochemical foundation of pathologic effects, which included membrane function decline, cell morphological change, configuration injuring and apoptosis etc. This paper is from a new view of protein conformation to explore the molecular pathologic mechanism of the damage caused by pulse microwave irradiation.
Deng
Hua
H
College of Life Science, Foshan Science Technology University, Foshan 528231, China.
Song
Zhan-Jun
ZJ
Wang
De-Wen
DW
Yang
Hong
H
Ma
Chun-Quan
CQ
Lu
Yu-Kui
YK
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D002478:Cells, Cultured; D008565:Membrane Proteins / Q000737:chemistry*; D051379:Mice; D008872:Microwaves; D032383:Myocytes, Cardiac / Q000737:chemistry* / Q000166:cytology / Q000528:radiation effects; D011487:Protein Conformation / Q000528:radiation effects; D017433:Protein Structure, Secondary / Q000528:radiation effects; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D008565:Membrane Proteins