title: [Evaluation of immunochromatography test for rapid detection of influenza A and B viruses using real-time PCR].
abstract: The sensitivity of rapid diagnostic kits to influenza B is lower than to influenza A. The cause-poor performance of the kit or the scarcity of viruses in type B specimens-has yet to be clarified. Using real-time PCR, we measured the amount of influenza viruses with nasopharyngeal aspirate fluid previously identified by virus isolation culture and passing the rapid diagnosis test by four types of kits, including the ESPLINE Influenza A&B-N (Fujirebio Corp., Japan). We classified the results of virus isolation and rapid diagnosis tests into three groups and examined them: group 1 (12 specimens, influenza B, all negative in tests using four types of kits); group 2 (57 specimens, influenza B, all positive in tests); and group 3 (36 specimens, AH3, all positive in tests). The average amount of viruses in group 1 (6.60 +/- 0.81 log10copies/mL) was significantly lower (p<0.0001) than that in group 2 (8.51 +/- 0.57 log10copies/mL) or group 3 (8.72 +/- 0.63 log10copies/mL). No significant difference was seen in the amount of viruses between groups 2 and 3. We concluded that the cause of low sensitivity in rapid diagnostic kits to influenza B are attributable to the scarcity of viruses in the specimen.
authors.affiliation: Hara Pediatric Clinic.
authors.forename: Michimaru
authors.initials: M
authors.lastname: Hara
authors.forename: Kenji
authors.initials: K
authors.lastname: Sadamasu
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takao
authors.forename: Takayuki
authors.initials: T
authors.lastname: Shinkai
authors.forename: Akemi
authors.initials: A
authors.lastname: Kai
authors.forename: Shinji
authors.initials: S
authors.lastname: Fukuda
authors.forename: Yukie
authors.initials: Y
authors.lastname: Shimazu
authors.forename: Masaru
authors.initials: M
authors.lastname: Kuwayama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Miyazaki
mesh_terms: D002675:Child, Preschool; D006801:Humans; D009980:Influenza A virus / Q000302:isolation & purification*; D009981:Influenza B virus / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis; D009305:Nasopharynx / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic