title: Gene expression in synovial membrane cells after intraarticular delivery of plasmid-linked superparamagnetic iron oxide particles--a preliminary study in sheep.
abstract: This study evaluated in vivo gene delivery and subsequent gene expression within cells of the synovium in the presence of static and pulsating magnetic field application following intraarticular injection of superparamagnetic iron oxide nanoparticles linked to plasmids containing reporter genes encoding for fluorescent proteins. Plasmids encoding genes for either green fluorescent protein or red fluorescent protein were bound to superparamagnetic nanoparticles coated with polyethyleneimine. Larger (200-250 nm) and smaller (50 nm) nanoparticles were compared to evaluate the effects of size on transfection efficiency as well as any associated intraarticular reaction. Comparisons between groups were evaluated at 24, 72, and 120 h time periods. Inflammatory response was mild to moderate for all injected particles, but was present in the majority of synovial membrane samples evaluated. Larger particles tended to be associated with more inflammation than smaller ones. Nevertheless, intraarticular application of both experimental and control nanoparticles were well tolerated clinically. Gene expression as determined by observation of either green or red intracellular fluorescence was difficult to assess by both epifluorescent light, and confocal microscopy. An insufficient concentration of nanoparticles in relation to joint volume likely resulted in a limited number of samples with positive evidence of iron staining and with suspected positive evidence of cells expressing fluorescent proteins. Our results indicate that intraarticular administration of functionalized superparamagnetic iron oxide nanoparticles resulted in a mild to moderate synovitis and there was in conclusive evidence of gene expression. Further research is warranted to determine the best and most effective reporter assay for assessment of the in vivo gene delivery into the joints. In addition, the best suited concentration and size of nanoparticles, which will optimize gene delivery and expression, while minimizing intraarticular inflammation, needs to be determined.
authors.affiliation: Musculoskeletal Research Unit, Equine Hospital, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
authors.forename: Larry D
authors.initials: LD
authors.lastname: Galuppo
authors.forename: Sarah W
authors.initials: SW
authors.lastname: Kamau
authors.forename: Benedikt
authors.initials: B
authors.lastname: Steitz
authors.forename: Paul O
authors.initials: PO
authors.lastname: Hassa
authors.forename: Monika
authors.initials: M
authors.lastname: Hilbe
authors.forename: Lloyd
authors.initials: L
authors.lastname: Vaughan
authors.forename: Sabine
authors.initials: S
authors.lastname: Koch
authors.forename: Alke
authors.initials: A
authors.lastname: Fink-Petri
authors.forename: Margarethe
authors.initials: M
authors.lastname: Hofman
authors.forename: Heinrich
authors.initials: H
authors.lastname: Hofman
authors.forename: Michael O
authors.initials: MO
authors.lastname: Hottiger
authors.forename: Brigitte
authors.initials: B
authors.lastname: von Rechenberg
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D015870:Gene Expression / Q000235:genetics; D007270:Injections, Intra-Articular; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics*; D012756:Sheep; D013583:Synovial Membrane / Q000502:physiology*; D014162:Transfection / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; C427127:superparamagnetic blood pool agent; D052203:Ferrosoferric Oxide