Endogenous thyroid hormone synthesis in facultative planktotrophic larvae of the sand dollar Clypeaster rosaceus: implications for the evolutionary loss of larval feeding.
Critical roles of hormones in metamorphic life history transitions are well documented in amphibians, lampreys, insects, and many plant species. Recent evidence suggests that thyroid hormones (TH) or TH-like compounds can regulate development to metamorphosis in echinoids (sea urchins, sand dollars, and their relatives). Moreover, previous research has provided evidence for endogenous hormone synthesis in both feeding and nonfeeding echinoderm larvae. However, the mechanisms for endogenous synthesis remain largely unknown. Here, we show that facultatively planktotrophic larvae (larvae that reach metamorphosis in the absence of food but have the ability to feed) from the subtropical sea biscuit Clypeaster rosaceus can synthesize thyroxine endogenously from incorporated iodine (I(125)). When treated with the goitrogen thiourea (a peroxidase inhibitor), iodine incorporation, thyroxine synthesis, and metamorphosis are all blocked in a dose-dependent manner. The inhibitory effect on metamorphosis can be rescued by administration of exogenous thyroxine. Finally, we demonstrate that thiourea induces morphological changes in feeding structures comparable to the phenotypic plastic response of larval structures to low food conditions, further supporting a signaling role of thyroxine in regulating larval morphogenesis and phenotypic plasticity. We conclude that upregulation of endogenous hormone synthesis might have been associated with the evolution of nonfeeding development, subsequently leading to morphological changes characteristic of nonfeeding development.
Heyland
Andreas
A
The Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL 32080, USA. aheyland@ufl.edu
Reitzel
Adam M
AM
Price
David A
DA
Moroz
Leonid L
LL
D000818:Animals; D005075:Biological Evolution; D004435:Eating; D007447:Invertebrate Hormones / Q000096:biosynthesis*; D007453:Iodide Peroxidase / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008675:Metamorphosis, Biological / Q000187:drug effects; D012617:Sea Urchins / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D015398:Signal Transduction; D013890:Thiourea / Q000494:pharmacology; D013963:Thyroid Hormones / Q000096:biosynthesis*; D013974:Thyroxine / Q000494:pharmacology
D007447:Invertebrate Hormones; D013963:Thyroid Hormones; D007453:Iodide Peroxidase; D013890:Thiourea; D013974:Thyroxine