Surfactant protein A promoter directs the expression of Cre recombinase in brain microvascular endothelial cells of transgenic mice.
Brain microvascular endothelial cells (ECs) have unique characteristics distinguished from peripheral ECs and play important roles in blood-brain barrier (BBB). To investigate the physiological control of the brain ECs, we generated a transgenic mouse line in which the expression of Cre recombinase was driven by the promoter of the mouse surfactant protein A (SP-A) gene. The Cre activity was detected in blood vessels of brain, alveolar type II cells of lung and epithelium of gland stomach. In brain ECs, the Cre activity started at embryonic day 11.5, indicating that the subpopulation of ECs in brain could be molecularly defined at early embryonic stages. The use of SP-A-Cre mice should facilitate analysis of gene function in the brain ECs.
Meng
Fanwei
F
Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing, PR China.
Shi
Ling
L
Cheng
Xuan
X
Hou
Ning
N
Wang
Yongliang
Y
Teng
Yan
Y
Meng
Anming
A
Yang
Xiao
X
D000818:Animals; D001808:Blood Vessels / Q000378:metabolism; D001812:Blood-Brain Barrier / Q000166:cytology / Q000378:metabolism; D001921:Brain / Q000098:blood supply; D042783:Endothelial Cells / Q000378:metabolism*; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D007763:Lac Operon / Q000235:genetics; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism; D037662:Pulmonary Surfactant-Associated Protein A / Q000235:genetics*; D051901:Smad4 Protein / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D037662:Pulmonary Surfactant-Associated Protein A; D051901:Smad4 Protein; C490810:Smad4 protein, mouse; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase