[Effects of Siwu decoction on bone marrow protein expression of blood deficiency mice induced by cyclophosphamide].
OBJECTIVE
To study the effects of Siwu decoction on protein expression of blood deficiency mice induced by cyclophosphamide (CIX) and discuss the possible molecular mechanism on blood enriching function of Siwu decoction.


METHODS
Blood deficiency mice were established by injecting ip with 250 mg x kg(-1) CTX. Proteomic technologies were applied to identify the different protein.


RESULTS
Siwu decoction could restore the changes of 12 up-regulated and 3 down-regulated proteins in bone marrow of blood deficiency mice induced by cyclosphosphamide.


CONCLUSIONS
Siwu decoction could effect expression of proteins which functions including apoptosis, proliferation and differentiation of the haematopoietic stem/progenitor cell. The regulation in the molecular level might be the mechanism of stimulating hematopoiesis in bone marrow fo siwu decocetion.
Liu
Li-Li
LL
Institute of Radiation Medicine, Beijing 100850, China.
Ma
Zeng-Chun
ZC
Wang
Yu-Guang
YG
Qin
Hong-Lin
HL
Tan
Hong-Ling
HL
Xiao
Cheng-Rong
CR
Gao
Yue
Y
D051339:Actin Depolymerizing Factors / Q000378:metabolism; D000740:Anemia / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D000818:Animals; D017305:Annexin A1 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002256:Carbonic Anhydrases / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003520:Cyclophosphamide; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005221:Fatigue / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000473:pathology; D008516:Medicine, Chinese Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D010544:Peroxidases / Q000378:metabolism; D054464:Peroxiredoxins; D010946:Plants, Medicinal / Q000737:chemistry; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
D051339:Actin Depolymerizing Factors; D017305:Annexin A1; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D020543:Proteome; C403258:si-wu-tang; D003520:Cyclophosphamide; D010544:Peroxidases; D054464:Peroxiredoxins; D002256:Carbonic Anhydrases