Sperm storage and arrangement within females of the arctiid moth Utetheisa ornatrix.
Female Utetheisa ornatrix mate prolifically, a behavior that accrues nuptially transferred gifts of nutrient and defensive alkaloids from males. This behavior also potentially places sperm from numerous males in competition within the female reproductive tract. Here, we investigate sperm interactions within female U. ornatrix by exploring the arrangement and numbers of sperm stored within the spermatheca and by examining sperm deposition in the pseudobursa, a presumed digestive organ in the female reproductive tract. Our results show that females store fewer sperm than they receive from their numerous mates, and the data suggest that unwanted sperm is either shunted to the pseudobursa or expelled from the spermatheca. We found no evidence that the apyrene, or non-nucleated, sperm morph common to the Lepidoptera are involved in forming barriers between ejaculates within the spermatheca. Female U. ornatrix are thus able to control sperm use, which we argue may contribute to the pattern of paternity observed in this species.
Curril
Ingrid M
IM
Cell and Molecular Biology Department, University of Vermont, Burlington, VT 05405, USA.
LaMunyon
Craig W
CW
D000818:Animals; D005260:Female; D005836:Genitalia, Female / Q000033:anatomy & histology / Q000502:physiology; D008297:Male; D009036:Moths / Q000033:anatomy & histology / Q000502:physiology*; D012098:Reproduction / Q000502:physiology; D013076:Sperm Count; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*