Evidence for pestivirus infection in free-living Japanese serows, Capricornis crispus.
Sixteen serum samples collected from free-living Japanese serows, Capricornis crispus, between 2001 and 2004 in Morioka and its vicinity were examined for the presence of pestivirus by reverse transcription-nested PCR procedure. Three out of the 16 samples produced a visible band in electrophoresed agarose gels. The nucleotide sequences of the three PCR products were found to be identical. The pestivirus found in the serow was identified as Bovine viral diarrhea virus 1 (BVDV-1) based on nucleotide sequence analyses by phylogeny as well as palindromic nucleotide substitutions at the 5' untranslated regions. Our data first indicated that BVDV-1 infection occurred continuously among the free-living serow populations though the role of BVDV-1 in wild ungulates is currently unknown.
Harasawa
Ryô
R
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan. harasawa-tky@umin.ac.jp
Aoyama
Fumio
F
Goryo
Masanobu
M
Okada
Kosuke
K
Nishimura
Takashi
T
Matsubara
Kazuei
K
Tsujimoto
Tsunenori
T
Giangaspero
Massimo
M
D000818:Animals; D000866:Antelopes / Q000821:virology*; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D007564:Japan; D018182:Pestivirus Infections / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction