Isolation and characterization of nickel uptake by nickel resistant bacterial isolate (NiRBI).
OBJECTIVE
Bioremediation technology has gained importance because microbes could be the convenient source of bio-absorption/bioaccumulation of metals from effluent streams.


METHODS
The nickel-resistant bacterial isolates (NiRBI) were selected from various bacterial isolates from industrial effluent and grown in nutrient broth containing different concentrations of nickel sulfate (0.3-3.0 mmol/L) and their capability of accumulating metal from the medium.


RESULTS
Well-defined growth of NiRBI was observed in the medium containing up to 2.5 mmol/L of nickel. The isolate was identified using 16S rRNA and closely related to Pseudomonas fragi. Maximum accumulation of nickel (0.59 mg/g dry weight of bacterial cells) was observed when NiRBI was grown in media containing 2 mmol/L of nickel. The protein profile of the NiRBI cellular extract by SDS-PAGE showed two metal stress-induced proteins of molecular weight 48 KD and 18 KD with a simultaneous down regulation of four proteins of 46.7 KD, 42.2 KD, 19.7 KD, and 4.0 KD.


CONCLUSIONS
48 KD and 18 KD proteins play a role in metal resistance mechanism by NiRBI.
Patel
Jagdish S
JS
Department of Biosciences, Sardar Patel University, Vallabh Vidyanagar-388 120, Gujarat, India.
Patel
Prerna C
PC
Kalia
Kiran
K
D001673:Biodegradation, Environmental; D004591:Electrophoresis, Polyacrylamide Gel; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D007700:Kinetics; D009532:Nickel / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000145:classification / Q000235:genetics
D012336:RNA, Ribosomal, 16S; C029938:nickel sulfate; D009532:Nickel