Specificity of DC-SIGN for mannose- and fucose-containing glycans.
The dendritic cell specific C-type lectin dendritic cell specific ICAM-3 grabbing non-integrin (DC-SIGN) binds to "self" glycan ligands found on human cells and to "foreign" glycans of bacterial or parasitic pathogens. Here, we investigated the binding properties of DC-SIGN to a large array of potential ligands in a glycan array format. Our data indicate that DC-SIGN binds with K(d)<2muM to a neoglycoconjugate in which Galbeta1-4(Fucalpha1-3)GlcNAc (Le(x)) trisaccharides are expressed multivalently. A lower selective binding was observed to oligomannose-type N-glycans, diantennary N-glycans expressing Le(x) and GalNAcbeta1-4(Fucalpha1-3)GlcNAc (LacdiNAc-fucose), whereas no binding was observed to N-glycans expressing core-fucose linked either alpha1-6 or alpha1-3 to the Asn-linked GlcNAc of N-glycans. These results demonstrate that DC-SIGN is selective in its recognition of specific types of fucosylated glycans and subsets of oligomannose- and complex-type N-glycans.
van Liempt
Ellis
E
Department of Molecular Cell Biology and Immunology, VU University Medical Center, Van der Boechorstsraat 7, 1081 BT Amsterdam, The Netherlands.
Bank
Christine M C
CM
Mehta
Padmaja
P
Garciá-Vallejo
Juan Jesús
JJ
Kawar
Ziad S
ZS
Geyer
Rudolf
R
Alvarez
Richard A
RA
Cummings
Richard D
RD
Kooyk
Yvette van
Yv
van Die
Irma
I
D015815:Cell Adhesion Molecules / Q000378:metabolism*; D005643:Fucose; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D008024:Ligands; D008358:Mannose; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D040081:Protein Array Analysis; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015815:Cell Adhesion Molecules; D005643:Fucose; D037181:Lectins, C-Type; D008024:Ligands; D008358:Mannose; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin