title: Characterization of a nonfimbrial mannose-sensitive hemagglutinin (MSH) produced by Salmonella enterica serovar enteritidis.
abstract: A nonfimbrial mannose-sensitive hemagglutinin (MSH) with adhesive properties produced by Salmonella enterica serovar Enteritidis was characterized. The MSH was characterized as glycoprotein and consisted of three noncovalently bound subunits of M(r) 28, 33 and 40 kDa determined by SDS-PAGE. The hemagglutinin was heat-stable and resistant to alkaline (high) or acid (low) pH, however, it was inhibited by proteolytic enzymes, by EDTA and by sodium periodate. Mouse antiserum raised against MSH reacted with the 28 kDa band in immunoblotting, and also inhibited hemagglutination and bacterial adherence to HeLa cells. Electron microscope examinations showed that MSH is not a fimbriae-like structure. MSH and anti-MSH IgG competitively inhibited bacterial adherence to HeLa cells. The immunofluorescence test, using MSH on HeLa cells and specific anti-MSH IgG, supported the view that MSH contributes to adherence of the organism. These results indicate that MSH is a nonfimbrial putative adhesive factor that may mediate the adherence of Salmonella enteritidis to eucaryotic cells.
authors.affiliation: Departamento de Análises Clínicas, Universidade Estadual de Maringá, Paraná, Brazil.
authors.forename: Jane M G
authors.initials: JM
authors.lastname: Mikcha
authors.forename: Maria G
authors.initials: MG
authors.lastname: Freire
authors.forename: Maria Ligia R
authors.initials: ML
authors.lastname: Macedo
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Yano
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Piantino Ferreira
mesh_terms: D018829:Adhesins, Bacterial / Q000302:isolation & purification*; D000818:Animals; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000302:isolation & purification*; D006801:Humans; D008358:Mannose / Q000494:pharmacology; D037241:Mannose-Binding Lectins / Q000302:isolation & purification*; D051379:Mice; D012477:Salmonella enteritidis / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; D006388:Hemagglutinins; D037241:Mannose-Binding Lectins; D008358:Mannose