Correlation of tissue selectin expression and hemodynamic parameters in rheumatic mitral valve disease.
OBJECTIVE
The study aim was to examine tissue expression of the adhesion molecules E-selectin and P-selectin on atrial, valvular and atrial myocardial blood vessel endothelium in patients with rheumatic mitral stenosis, and to investigate whether such expression was correlated with hemodynamics.


METHODS
Thirteen patients (eight women, five men; mean age 51 +/- 10 years) with severe rheumatic mitral stenosis who underwent mitral valve replacement surgery were examined on preoperative day 1, using cardiac catheterization and echocardiography. Specimens from the mitral valve and left atrium of each patient were evaluated for CD 62E and CD 62P expression using indirect immunoperoxidase and immunofluorescence techniques


RESULTS
A great majority of patients presented E and/or P selectin expression of variable intensity on atrial, valvular and atrial myocardial blood vessel endothelium. A more diffuse and stronger reaction for CD 62P was noted compared to that for CD 62E. The left ventricular end-diastolic diameter and left atrial diameter were positively correlated with endocardial CD 62P and CD 62E expression. Right atrial pressure was also strongly and positively correlated with endocardial expression of CD 62E (r = 0.80, p 0.03) and CD 62P (r = 0.8, p = 0.02).


CONCLUSIONS
Marked tissue expression of CD 62E and CD 62P was identified on atrial, valvular and atrial myocardial blood vessel endothelium. Moreover, the degree of expression of adhesion molecules was significantly correlated with the left atrial and left ventricular chamber diameters, as well as right atrial pressure.
Tokgözoglu
Lale
L
Hacettepe University Faculty of Medicine, Department of Cardiology, 06100 Sihhiye, Ankara, Turkey. lalet@hacettepe.edu.tr
Can
Ilknur
I
Korkusuz
Petek
P
Asan
Esin
E
Ozer
Necla
N
Demircin
Metin
M
D000328:Adult; D016280:Atrial Function, Right; D001794:Blood Pressure; D006328:Cardiac Catheterization; D019040:E-Selectin / Q000378:metabolism*; D004452:Echocardiography; D004730:Endothelium, Vascular / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006325:Heart Atria / Q000378:metabolism; D019918:Heart Valve Prosthesis Implantation; D006352:Heart Ventricles / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D008944:Mitral Valve Insufficiency / Q000378:metabolism / Q000503:physiopathology; D008946:Mitral Valve Stenosis / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology* / Q000601:surgery; D019007:P-Selectin / Q000378:metabolism*; D012107:Research Design; D012214:Rheumatic Heart Disease / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology* / Q000601:surgery; D012720:Severity of Illness Index; D013318:Stroke Volume; D016896:Treatment Outcome
D019040:E-Selectin; D019007:P-Selectin