title: Age- and gender-related oxidative status determined in healthy subjects by means of OXY-SCORE, a potential new comprehensive index.
abstract: Oxidative stress has been related to various diseases, gender and ageing, and has been measured by various markers. The authors developed a procedure to compute a global oxidative stress index (OXY-SCORE), reflecting both oxidative and antioxidant markers in healthy subjects. Its performance was tested in relation to age and gender and in coronary artery disease (CAD) patients. Eighty-two healthy subjects and 20 CAD patients were enrolled. Plasma free and total malondialdehyde (F- and T-MDA), glutathione disulphide/reduced form ratio (GSSG/GSH) and urine isoprostanes (iPF2alpha-III) levels were combined as oxidative damage markers (damage score). GSH, alpha- and gamma-tocopherol (TH) levels, and individual antioxidant capacity were combined as antioxidant defence indexes (protection score). The OXY-SCORE was computed by subtracting the protection score from the damage score. Among single parameters, T-MDA and iPF2alpha-III significantly correlated with age; only GSH and both tocopherols correlated with male gender in healthy subjects. The OXY-SCORE was positively associated with age (p=0.004) and male gender (p=0.03). As expected, the OXY-SCORE was higher in CAD with a very significant p-value (<0.0001), after adjusting for age, gender and smoking. Combining different markers can potentially provide a powerful index in the evaluation of oxidative stress related to age, gender and CAD status.
authors.affiliation: Centro Cardiologico Monzino I.R.C.C.S., Milan, Italy. fabrizio.veglia@ccfm.it
authors.forename: F
authors.initials: F
authors.lastname: Veglia
authors.forename: G
authors.initials: G
authors.lastname: Cighetti
authors.forename: M
authors.initials: M
authors.lastname: De Franceschi
authors.forename: L
authors.initials: L
authors.lastname: Zingaro
authors.forename: L
authors.initials: L
authors.lastname: Boccotti
authors.forename: E
authors.initials: E
authors.lastname: Tremoli
authors.forename: V
authors.initials: V
authors.lastname: Cavalca
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000032:analysis; D015415:Biomarkers / Q000097:blood / Q000652:urine; D003324:Coronary Artery Disease / Q000175:diagnosis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016877:Oxidants / Q000032:analysis; D018384:Oxidative Stress; D012720:Severity of Illness Index; D012737:Sex Factors
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016877:Oxidants