[Mechanism of Shenqi compound recipe anti-earlier diabetic artherosclerosis in GK rats].
OBJECTIVE
To explore the mechanism of Shenqi compound recipe (SQCR) anti-earlier diabetic artherosclerosis in GK rats.


METHODS
Four-month specefic pathogen free (SPF) GK rats were divided randomly according to blood glucose level into four groups: model group (5 mL x kg(-1) x d(-1) sterile water), ramipril group (positive control, 1 mg x kg(-1) x d(-1)), SQCR low dosage (0.72 g x kg(-1) x d(-1)) and SQCR high dosage group (2.88 g x kg(-1) x d(-1)) and Wistar rats as normal control group(5 mL x kg(-1) x d(-1) sterile water). GK rats took high-fat diet freely and meanwile were injected N-omega-nitro-L-arginine methyl ester (L-N-AME) intra-peritoneally with the dose of 10 mg x kg(-1) x d(-1) in order to induce earlier diabetic artherosclerosis, while normal control group took regular diet and were injected normal saline intra-peritoneally. In the experiment periods, each group was administrated correspondent substance respectively for 32 d. At the end, sampling blood by abdominal aorta and picking aorta on ice. Determined monocyte chemoattractant protein-1 (MCP-1) concentration by ELISA, messenger ribonucleic acid (mRNA) expression of MCP-1 and peroxisome proliferator-activated receptor gamma (PPARgamma) in aorta by reverse transcriptase PCR (RT-PCR).


RESULTS
Concentrations of MCP-1 in serum in SQCR low and high dosage groups and the mRNA expression of MCP-1 in SQCR high dosage group were all decreased significantly compared with model group (P < 0.05). The mRNA expression of PPARgamma in SQCR low and high dosage groups all increased compared with model group (P < 0.05 or P < 0.01).


CONCLUSIONS
Inhibiting the mRNA and protein expression of MCP-1 and upregulating the mRNA expression of PPARgamma in aorta might be contribute to SQCR anti-earlier diabetic artherosclerosis in GK rats partly.
Zhang
Hong-min
HM
Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu.
Chen
Shi-wei
SW
Xie
Chun-guang
CG
Xie
Yi-qiang
YQ
Deng
Xi-fang
XF
D000818:Animals; D001011:Aorta / Q000378:metabolism; D027885:Astragalus propinquus / Q000737:chemistry; D050197:Atherosclerosis / Q000209:etiology / Q000378:metabolism*; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D047495:PPAR gamma / Q000096:biosynthesis / Q000235:genetics; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D047495:PPAR gamma; D012333:RNA, Messenger