Development of an immunochromatographic test kit for rapid detection of bovine viral diarrhea virus antigen.
An immunochromatographic test was developed for rapid diagnosis of bovine viral diarrhea virus (BVDV) infections using monoclonal antibodies against the nonstructural protein, NS3, of the virus. The kit detected specifically the NS3 of various BVDV strains. Using the kit, leukocyte extracts of cattle infected persistently with BVDV were found positive while those of healthy cattle were negative. The sensitivity and specificity of this kit in compared with virus isolation were 100% and 97.2%, respectively. Furthermore, the test also gave positive results for calves infected acutely with BVDV in experimental infection. The BVDV antigen was detected in 1 ml of blood using a relatively simple procedure. This test kit should be useful for rapid diagnosis of BVD.
Kameyama
K
K
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Sapporo 060-0818, Japan.
Sakoda
Y
Y
Tamai
K
K
Igarashi
H
H
Tajima
M
M
Mochizuki
T
T
Namba
Y
Y
Kida
H
H
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001769:Blood / Q000821:virology; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis*; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology* / Q000302:isolation & purification*; D007962:Leukocytes / Q000821:virology; D010447:Peptide Hydrolases / Q000032:analysis*; D020365:RNA Helicases / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D017361:Viral Nonstructural Proteins / Q000032:analysis*; D014776:Virus Cultivation; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D000956:Antigens, Viral; D010447:Peptide Hydrolases; D020365:RNA Helicases; D011933:Reagent Kits, Diagnostic; D017361:Viral Nonstructural Proteins; C086730:p80 protein, bovine viral diarrhea virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases