title: Variation in the content of hypericins in four generations of seed progeny of Hypericum perforatum somaclones.
abstract: The content of hypericins in in vitro regenerated Hypericum perforatum plants (R (0)) and four generations of their seed progeny (R (1)-R (4)) was compared. The mean content of hypericins in field-grown plants over the period 1992-2002 gradually increased under selection, and in the R (4) generation it was almost seven-times higher than that in the R (0) somaclones. Significant difference between hypericin content in diploids and tetraploids was detected in R (0), R (1) and R (3) generations. Hypericin content in four diploid and tetraploid lineages originated from a single somaclone was genotype dependent. To eliminate the influence of environmental conditions during different growing seasons, we used seeds of selected R (0)-R (3) plants to derive R'(1) to R'(4) generations cultivated during the same years. In this case no statistically significant difference in hypericin content was found between the R'(1)-R'(4) generations. Apomictically and sexually derived plants were distinguished by PCR using variable numbers of tandem repeats (VNTR) primers. The content of hypericins in apomictically derived progenies was compared.
authors.affiliation: Faculty of Science, Institute of Biology and Ecology, P. J. Safárik University, Mánesova, Kosice, Slovakia. jana.koperdakova@upjs.sk
authors.forename: Jana
authors.initials: J
authors.lastname: Koperdáková
authors.forename: Ján
authors.initials: J
authors.lastname: Kosuth
authors.forename: Eva
authors.initials: E
authors.lastname: Cellárová
mesh_terms: D000873:Anthracenes; D001483:Base Sequence; D032461:Chromosomes, Plant; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation; D020902:Hypericum / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D018598:Minisatellite Repeats; D010569:Perylene / Q000031:analogs & derivatives* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012639:Seeds / Q000378:metabolism*
chemical_list: D000873:Anthracenes; D017931:DNA Primers; D018744:DNA, Plant; D010569:Perylene; C004965:hypericin