Effect of cooling on electrical field stimulation and norepinephrine-induced contraction in isolated hypertrophic human nasal mucosa.
BACKGROUND
Exposure to cold causes cutaneous vasoconstriction to reduce body heat loss, while the nasal cavity warms up the inspired cold air. This suggests cooling might evoke a different response in nasal mucosa blood vessels than in cutaneous blood vessels. Thus, the aim of this study was to evaluate the effect of cooling (to 24 degrees C) on the vascular response of isolated nasal mucosa.


METHODS
An in vitro isometric contraction of nasal mucosa excised from patients suffered from chronic nasal congestion was continuously recorded. Either electrical field stimulation (EFS) or exogenous norepinephrine (NE) was applied to the turbinate mucosa strip at 37 and 24 degrees C, and the influence of cooling (to 24 degrees C) was evaluated.


RESULTS
EFS (1, 2, 4, and 8 Hz) produced frequency-dependent contractions at 37 and 24 degrees C. Cooling did not alter the magnitude but significantly prolonged the duration of EFS-induced contraction. Exogenous NE (10(-8) to 10(-4) M) produced concentration-dependent contractions at 37 and 24 degrees C. Cooling significantly enhanced the contractile responses evoked by NE between 3 x 10(-6) and 1 x 10(-4) M.


CONCLUSIONS
Cooling (to 24 degrees C) prolonged the EFS-induced and enhanced NE (3 x 10(-6) to 1 x 10(-4) M)-induced contraction of isolated human nasal mucosa.
Chu
Yueng-Hsiang
YH
Graduate Institute of Medical Sciences, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Wu
Chin-Chen
CC
Wang
Hsing-Won
HW
D000293:Adolescent; D000328:Adult; D003080:Cold Temperature; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005260:Female; D006801:Humans; D006984:Hypertrophy; D007537:Isometric Contraction / Q000187:drug effects / Q000502:physiology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D009297:Nasal Mucosa / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology*; D009638:Norepinephrine / Q000494:pharmacology*; D014420:Turbinates / Q000166:cytology; D014661:Vasoconstriction / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
D014662:Vasoconstrictor Agents; D009638:Norepinephrine