title: Precipitation complexation method produces cannabidiol/beta-cyclodextrin inclusion complex suitable for sublingual administration of cannabidiol.
abstract: In the present study, the precipitation complexation method was used to prepare a complex of cannabidiol (CBD) with beta-CD. The effect of beta-CD-complexation on the sublingual absorption of CBD was studied in rabbits. A solid CBD/beta-CD inclusion complex was prepared by precipitation and the effect of complex formation on the dissolution rate of CBD was studied. The absorption of CBD (a 250 microg/kg dose of CBD in all formulations) after sublingual administration of solid CBD/beta-CD complex and ethanolic CBD solution, and after oral administration of ethanolic CBD solution, was studied in vivo in rabbits. The dissolution rate of solid CBD/beta-CD complex in vitro was significantly (p<0.05) higher than that of plain CBD. The absorption of CBD (AUC0-300 min) decreased in the following order: sublingual ethanolic CBD solution (420+/-120 ngxmin/mL; mean+/-SD; n=4)>sublingual solid CBD/beta-CD complex (270+/-120 ngxmin/mL)>oral ethanolic CBD solution (concentrations in plasma below the quantitation limit). The results demonstrate that sublingual administration of a solid CBD/beta-CD complex enhances the absorption of CBD in rabbits when compared to oral administration of ethanolic CBD. Furthermore, the solid CBD/beta-CD complex may provide an alternative formulation for sublingual administration of CBD.
authors.affiliation: Department of Pharmaceutical Chemistry, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland. janne.mannila@uku.fi
authors.forename: Janne
authors.initials: J
authors.lastname: Mannila
authors.forename: Tomi
authors.initials: T
authors.lastname: Järvinen
authors.forename: Kristiina
authors.initials: K
authors.lastname: Järvinen
authors.forename: Pekka
authors.initials: P
authors.lastname: Jarho
mesh_terms: D000042:Absorption; D000284:Administration, Oral; D000286:Administration, Sublingual; D000818:Animals; D001682:Biological Availability; D002185:Cannabidiol / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D011232:Chemical Precipitation; D004339:Drug Compounding; D000431:Ethanol / Q000737:chemistry; D005260:Female; D008297:Male; D011817:Rabbits; D012995:Solubility; D047392:beta-Cyclodextrins / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; D002185:Cannabidiol; D000431:Ethanol; C031215:betadex