title: Quantitative analysis of the expression of glycosylphosphatidylinositol-anchored proteins during the maturation of different hematopoietic cell compartments of normal bone marrow.
abstract: BACKGROUND
  Glycosylphosphatidylinositol-anchored proteins (GPI-AP) are a heterogeneous group of proteins deficiently expressed in patients with paroxysmal nocturnal hemoglobinuria. Up till now, no study has been reported in which the exact patterns of expression of a large number of GPI-AP are quantitatively evaluated in normal bone marrow (BM) cells classified according to their lineage and maturation stage.
  
  
  METHODS
  In the present study, we have quantitatively analyzed the expression of eleven different GPI-AP (CD14, CD16, CD24, CD48, CD52, CD58, CD59, CD66b, CD87, CD109 and CD157) during maturation of the neutrophil, monocytic, erythroid, lymphoid, basophil and plasmacytoid dendritic cells (DC) lineages in normal BM as a frame of reference for the understanding of the abnormal patterns of expression of GPI-AP observed in the BM of PNH patients.
  
  
  RESULTS
  Our results show that expression of most GPI-AP varies during normal BM maturation, different profiles being frequently observed depending on the cell lineage or the GPI-AP analyzed.
  
  
  CONCLUSIONS
  Overall, these results provide a detailed map GPI-AP expression during normal hematopoietic differentiation, which could serve as a basis for the identification and characterization of changes occurring in PNH.
authors.affiliation: Servicio General de Citometría and Departamento de Medicina and Centro de Investigación del Cáncer, Universidad de Salamanca, Paseo San Vicente 58-182, 37007 Salamanca, Spain.
authors.forename: Pilar María
authors.initials: PM
authors.lastname: Hernández-Campo
authors.forename: Julia
authors.initials: J
authors.lastname: Almeida
authors.forename: Sergio
authors.initials: S
authors.lastname: Matarraz
authors.forename: María
authors.initials: M
authors.lastname: de Santiago
authors.forename: María Luz
authors.initials: ML
authors.lastname: Sánchez
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology; D001491:Basophils / Q000166:cytology; D001854:Bone Marrow Cells / Q000166:cytology*; D002451:Cell Compartmentation; D002454:Cell Differentiation; D019070:Cell Lineage; D003713:Dendritic Cells / Q000166:cytology; D041905:Erythroid Cells / Q000166:cytology; D005260:Female; D017261:Glycosylphosphatidylinositols / Q000032:analysis*; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology; D009504:Neutrophils / Q000166:cytology
chemical_list: D015703:Antigens, CD; D017261:Glycosylphosphatidylinositols