title: Five years' experience of preimplantation genetic diagnosis in the Parisian Center: outcome of the first 441 started cycles.
abstract: OBJECTIVE
  To investigate the evolution of techniques and strategies and to evaluate the results of preimplantation genetic diagnosis (PGD) from January 2000 to December 2004 in chromosomal, monogenic and mitochondrial DNA disorders treated at our institution.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  Single French Parisian PGD center.
  
  
  METHODS
  Patients at risk of transmitting a serious genetic disorder to their offspring.
  
  
  METHODS
  171 couples enrolled in the program undergoing stimulated and frozen embryo replacement cycles with PGD.
  
  
  METHODS
  Results of the 441 first PGD cycles performed for various genetic conditions.
  
  
  RESULTS
  During 5 years, 416 stimulation and 25 frozen embryo replacement cycles were started, among which 52 clinical and 47 ongoing pregnancies occurred. In stimulation cycles, the overall ongoing pregnancy rate was 24% per embryo transfer, 11% per started cycle, and 27% per couple. The implantation rate was 16%.
  
  
  CONCLUSIONS
  These encouraging results demonstrate that PGD might be considered as a valid alternative to prenatal diagnosis. Nevertheless, couples referred for PGD must be selected and counseled appropriately, considering the complexity of the treatment and the relatively low take-home baby rate.
authors.affiliation: Service de Gynécologie-Obstétrique et de Médecine de la Reproduction, Hôpital Antoine Béclère, Clamart, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Feyereisen
authors.forename: Julie
authors.initials: J
authors.lastname: Steffann
authors.forename: Serge
authors.initials: S
authors.lastname: Romana
authors.forename: Marc
authors.initials: M
authors.lastname: Lelorc'h
authors.forename: Pierre
authors.initials: P
authors.lastname: Ray
authors.forename: Violaine
authors.initials: V
authors.lastname: Kerbrat
authors.forename: Gérard
authors.initials: G
authors.lastname: Tachdjian
authors.forename: René
authors.initials: R
authors.lastname: Frydman
authors.forename: Nelly
authors.initials: N
authors.lastname: Frydman
mesh_terms: D000328:Adult; D005260:Female; D005602:France / Q000453:epidemiology; D040181:Genetic Diseases, X-Linked / Q000175:diagnosis* / Q000453:epidemiology* / Q000235:genetics / Q000517:prevention & control; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000235:genetics; D006801:Humans; D008137:Longitudinal Studies; D008875:Middle Aged; D017063:Outcome Assessment, Health Care / Q000379:methods* / Q000706:statistics & numerical data; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000453:epidemiology*; D019836:Preimplantation Diagnosis / Q000706:statistics & numerical data*; D011379:Prognosis; D015203:Reproducibility of Results; D012189:Retrospective Studies; D018570:Risk Assessment / Q000379:methods*; D012307:Risk Factors; D012680:Sensitivity and Specificity; D016896:Treatment Outcome