Transfer of bacteria between biomaterials surfaces in the operating room--an experimental study.
Bacterial adhesion to and transfer between surfaces is a complicated process. With regard to the success of biomaterials implants, studies on bacterial adhesion and transfer should not be confined to biomaterials surfaces in the human body, but also encompass surfaces in the operating room, where the origin of many biomaterials related infections is found. The purpose of this study was to quantify the transfer of Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes from one operating room material to another, while accounting for surface hydrophobicity and roughness, moistness and application of friction during transfer. The tested operating room materials were gloves, broaches (orthopaedic drills), theatre gowns, and light handles. As a possible clinical intervention method to prevent transfer, it was investigated whether dipping the gloves in a chlorhexidine splash-basin affected the viability of the transferred bacteria. Transfer (moist and without friction) was demonstrated to some extent with all bacterial strains and with every material, ranging from 17% to 71%, and was influenced by the bacterial strain, moistness of the inoculum, the application of friction, and the characteristics of both the donating and the receiving surface. Dipping the glove material in 4% or 0.4% chlorhexidine solutions killed all bacteria present, regardless of whether surfaces were dried or moist and thus prevented transfer.
Knobben
Bas A S
BA
Department of Biomedical Engineering, University Medical Center Groningen, and University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
van der Mei
Henny C
HC
van Horn
Jim R
JR
Busscher
Henk J
HJ
D001419:Bacteria / Q000254:growth & development; D001422:Bacterial Adhesion; D001424:Bacterial Infections / Q000517:prevention & control*; D001672:Biocompatible Materials; D002710:Chlorhexidine; D015169:Colony Count, Microbial; D004202:Disinfectants; D006801:Humans; D017053:Infection Control; D008422:Materials Testing; D009873:Operating Rooms; D011481:Protective Clothing / Q000382:microbiology; D013499:Surface Properties
D001672:Biocompatible Materials; D004202:Disinfectants; D002710:Chlorhexidine