Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability.
Reetz
Manfred T
MT
Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim/Ruhr, Germany. reetz@mpi-muelheim.mpg.de
Carballeira
José D
JD
Vogel
Andreas
A
D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D018360:Crystallography, X-Ray; D019020:Directed Molecular Evolution / Q000379:methods*; D004795:Enzyme Stability / Q000235:genetics / Q000502:physiology*; D007700:Kinetics; D008958:Models, Molecular; D016296:Mutagenesis; D011489:Protein Denaturation; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D013379:Substrate Specificity; D013696:Temperature; D013816:Thermodynamics
D001426:Bacterial Proteins; C079999:LipA protein, Bacteria; D011506:Proteins