Generation of HIV-1 derivatives that productively infect macaque monkey lymphoid cells.
The narrow host range of human immunodeficiency virus type 1 (HIV-1) is caused in part by innate cellular factors such as apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G) and TRIM5alpha, which restrict virus replication in monkey cells. Variant HIV-1 molecular clones containing both a 21-nucleotide simian immunodeficiency virus (SIV) Gag CA element, corresponding to the HIV-1 cyclophilin A-binding site, and the entire SIV vif gene were constructed. Long-term passage in a cynomolgus monkey lymphoid cell line resulted in the acquisition of two nonsynonymous changes in env, which conferred improved replication properties. A proviral molecular clone, derived from infected cells and designated NL-DT5R, was used to generate virus stocks capable of establishing spreading infections in the cynomolgus monkey T cell line and CD8-depleted peripheral blood mononuclear cells from five of five pig-tailed macaques and one of three rhesus monkeys. NL-DT5R, which genetically is >93% HIV-1, provides the opportunity, not possible with currently available SIV/HIV chimeric viruses, to analyze the function of multiple HIV-1 genes in a broad range of nonhuman primate species.
Kamada
Kazuya
K
Department of Virology, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima-shi, Tokushima 770-8503, Japan.
Igarashi
Tatsuhiko
T
Martin
Malcolm A
MA
Khamsri
Boonruang
B
Hatcho
Kazuki
K
Yamashita
Tomoki
T
Fujita
Mikako
M
Uchiyama
Tsuneo
T
Adachi
Akio
A
D000071479:APOBEC-1 Deaminase; D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D002460:Cell Line; D003564:Cytidine Deaminase / Q000037:antagonists & inhibitors / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D016341:Genes, vif; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D008214:Lymphocytes / Q000821:virology*; D008251:Macaca / Q000821:virology*; D008969:Molecular Sequence Data; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology; D014779:Virus Replication
D036022:Capsid Proteins; D004279:DNA, Viral; D000071479:APOBEC-1 Deaminase; C000605089:APOBEC1 protein, human; D003564:Cytidine Deaminase