title: Effect of immunisation with cancer procoagulant on the growth of Walker 256 carcinosarcoma cells in rats.
abstract: The effect of preimmunisation with cancer procoagulant (CP) on the growth of Walker 256 carcinosarcoma cells in Wistar Lew/Han/IMP rats in vivo has been analysed. One group of rats was immunised with CP purified from human amnion-chorion membranes. The rest of the animals (control groups) were injected with 0.9% NaCl in Freund's adjuvant or were not immunised at all. When the presence of polyclonal anti-CP antibody was detected in CP-immunised rats' serum, all (CP-immunised and control) animals were injected i.p. with 4.8 x 10(5) Walker 256 cells per rat. After 5 days ascitic fluid was collected and viable cells were counted. The complete lack of viable Walker 256 carcinosarcoma cells in 24% of the CP-immunised rats was observed. However, the viable neoplastic cells were present in all control (NaCl-injected and nonimmunised) animals. It seems possible that CP plays an important role in tumour progression, so immunisation with CP can reduce the risk of development of malignant disease.
authors.affiliation: Department of Pharmaceutical Biochemistry, Medical University of Lodz, ul. Muszynskiego 1, PL-90151, Lodz, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kamocka
authors.forename: K
authors.initials: K
authors.lastname: Kaplinska
authors.forename: W P
authors.initials: WP
authors.lastname: Mielicki
mesh_terms: D000818:Animals; D002279:Carcinoma 256, Walker / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D003546:Cysteine Endopeptidases / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007114:Immunization; D008297:Male; D009363:Neoplasm Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009363:Neoplasm Proteins; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant