[Study of DNA damage induced by potassium dichromate and glutathione with atomic force microscope].
OBJECTIVE
To observe calf thymus DNA damage induced by potassium dichromate in combination with glutathione (GSH).


METHODS
Atomic force microscope and ultraviolet spectrum (UV) were used to observe the alterations of the DNA ultrastructure and absorption spectrum.


RESULTS
Atomic force microscopy revealed no breaks of the DNA strand in response to treatment with potassium dichromate alone, but when coupled with GSH at proper concentrations, potassium dichromate induced alterations in the DNA structure and DNA fragmentation. UV examination also confirmed these findings by showing increased absorption intensity of the maximum UV peak following combined treatment of the DNA with potassium dichromate and GSH.


CONCLUSIONS
These morphological and spectrographic evidences verified the important role of GSH in mediating the generation of various tumor-inducing intermediate products of potassium dichromate.
Ma
Yu-feng
YF
Department of Chemistry, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. happymyf@tom.com
Cai
Ji-ye
JY
Zhao
Qiu-xiang
QX
Yang
Pei-hui
PH
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004249:DNA Damage; D053938:DNA Fragmentation / Q000187:drug effects; D005978:Glutathione / Q000633:toxicity*; D018625:Microscopy, Atomic Force / Q000379:methods*; D009690:Nucleic Acid Conformation / Q000187:drug effects; D011192:Potassium Dichromate / Q000633:toxicity*; D013056:Spectrophotometry, Ultraviolet
D004247:DNA; C427203:calf thymus DNA; D005978:Glutathione; D011192:Potassium Dichromate