[Effects of paeoniflorin on cerebral energy metabolism, nitric oxide and nitric oxide synthase after cerebral ischemia in mongoliagerbils].
OBJECTIVE
To explore the effects of paeoniflorin on antagonising the delayed neuronal death (DND) induced by cerebral ischemia,and the relation between DND, cerebral tissue energy metabolism, nitric oxide (NO) and nitric oxide synthase (NOS).


METHODS
Incomplete cerebral ischemia induced was induced by ligating bilateral arteries carotis communis for 20 min followed by reperfusion 48 h in rats. The indexes including Na(+)-K(+)-ATPase activity, lactic acid content, Ca(2+)-ATPase, Mg(2+)-ATPase activity, NO content and NOS activity were determined in fore brain cortex at 48 h after reperfusion.


RESULTS
Na(+)-K(+)-ATPase, Ca(2+)-ATPase and Mg(2+)-ATPase activity were lowered (P < 0.01), NO level was decreased (P < 0.01), NOS activity dropped (P < 0.01) in cerebral tissue at 48h after reperfusion, but lactic acid level had no change. Paeoniflorin could prevent reduction of Na(+)-K(+)-ATPase activity (P < 0.05, P < 0.01), increase NO level (P < 0.01), enhance NOS activity (P < 0.01) at 48h after reperfusion.


CONCLUSIONS
DND induced by ischemia may be concerned with energy metabolism disorder and decrease of NO formation. Paeoniflorin may play the role of antagonising cerebral ischemia by adjusting cerebral energy metabolism and nitric oxide formation.
Sun
Rong
R
Department of pharmacology, China Pharmaceutical University, Nanjing 210009, China.
Lv
Li-li
LL
Liu
Guo-qing
GQ
D000818:Animals; D001565:Benzoates / Q000302:isolation & purification / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000150:complications; D001952:Bridged-Ring Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D005849:Gerbillinae; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D039821:Monoterpenes; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D005960:Glucosides; D019344:Lactic Acid; D039821:Monoterpenes; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D000254:Sodium-Potassium-Exchanging ATPase; C015423:peoniflorin; D006027:Glycosides