Induction of aquaporin 1 by dexamethasone in lipid rafts in immortalized brain microvascular endothelial cells.
Water homeostasis in the brain is essential for brain function. We have studied how aquaporin (AQP) 1 expression in GP8 immortalized rat brain microvascular endothelial cells is regulated by glucocorticoid. AQP1 protein level was raised by dexamethasone treatment in a time- and concentration-dependent manner. The up-regulation of AQP1 protein by dexamethasone was associated with an increase of AQP1 mRNA level, with no change in the degradation rate of AQP1 mRNA. AQP1 was concentrated in detergent-insoluble fractions in the cells treated with or without dexamethasone, suggesting that function/trafficking of AQP1 may be regulated via the interaction with lipid rafts. Since glucocorticoid therapy has well known beneficial effects in the treatment of brain edema, the induction of AQP1 by dexamethasone raises a possibility that AQP1 plays a role in ameliorating brain edema.
Kobayashi
Hideyuki
H
Department of Pharmacology, Miyazaki Medical College, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. hkobayas@med.miyazaki-u.ac.jp
Yokoo
Hiroki
H
Yanagita
Toshihiko
T
Satoh
Shinya
S
Kis
Bela
B
Deli
Maria
M
Niwa
Masami
M
Wada
Akihiko
A
D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D001921:Brain / Q000098:blood supply / Q000166:cytology; D002196:Capillaries / Q000166:cytology / Q000378:metabolism; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005938:Glucocorticoids / Q000494:pharmacology*; D008297:Male; D021962:Membrane Microdomains / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D015854:Up-Regulation
D005938:Glucocorticoids; D012333:RNA, Messenger; D051398:Aquaporin 1; D003907:Dexamethasone