Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates.
A multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.
Vilardo
Mônica de Castro Britto
Mde C
Laboratório de Zoonoses Bacterianas, Departamento de Bacteriologia, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil
Thomé
Jacqueline Darc da Silva
JD
Esteves
Wagner Thadeu Cardoso
WT
Filgueiras
Ana Luzia Lauria
AL
de Oliveira
Selma Soares
SS
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D002167:Campylobacter / Q000145:classification* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis*; D005838:Genotype; D008252:Macaca fascicularis; D008253:Macaca mulatta; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D012453:Saimiri
D004269:DNA, Bacterial