Antioxidant activity of isoflavones and biflavones isolated from Godoya antioquiensis.
Two biflavones, ochnaflavone (1) and 2",3"-dihydroochnaflavone (2), and two isoflavones, 5,7,4'-trihydroxy-3',5'-dimethoxyisoflavone (piscigenin) (3) and 5,4'-dihydroxy-7,3',5'-trimethoxyisoflavone (4), a new natural compound, were isolated from the leaves of Godoya antioquiensis (Ochnaceae). Their structures were determined on the basis of spectral data and by comparison with data reported in the literature. The isolated compounds were evaluated for their radical scavenging activity using the NBT (nitrobluetetrazolium)/hypoxanthine superoxide and the .OH/luminol chemiluminescence methods. The isolated isoflavones were found to exhibit a strong hydroxyl radical scavenging activity and a moderate inhibition of the superoxide anion, whereas the two biflavones were inactive in the superoxide anion assay and showed a low hydroxyl radical scavenging activity.
Benedek
Birgit
B
Pharmacognosie et Molécules Naturelles Bioactives, UMR 7081, Faculté de Pharmacie, Université Louis Pasteur de Strasbourg, Illkirch, France.
Weniger
Bernard
B
Parejo
Irene
I
Bastida
Jaume
J
Arango
Gabriel Jaime
GJ
Lobstein
Annelise
A
Codina
Caries
C
D000975:Antioxidants; D001713:Biphenyl Compounds; D002855:Chromatography, Thin Layer; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D007202:Indicators and Reagents; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D043842:Ochnaceae / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D047309:Flavones; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D007529:Isoflavones; D010851:Picrates; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl