[Determination of akebia saponin D in root of Dipacus asperoides by HPLC].
OBJECTIVE
To develop a quantitative method for the determination of akebia saponin D in root of Dipacus asperoides and provide scientific basis for quality control of D. asperoides.


METHODS
An IPLC analytical method was established using Kromasil ODS column with acetonitrile-water (30:70) as mobile phase and the detection wavelength was 212 nm.


RESULTS
The akebia saponin D in root extract were well separated by this method. The linear range is between 0.582 5-9.32 microg, r = 0.9999. The average recovery and RSD of repeatability are 100.3%, 2.3%, respectively.


CONCLUSIONS
This method is simple, accurate and reliable by evaluating the method validation data and can be used for the quality control of D. asperoides and its preparations.
Tan
Hong-gen
HG
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing.
Lin
Sheng
S
Zhang
Qi-wei
QW
Ji
Li
L
D002681:China; D002851:Chromatography, High Pressure Liquid; D049917:Dipsacaceae / Q000737:chemistry*; D017753:Ecosystem; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012503:Saponins / Q000032:analysis*
D012503:Saponins