Vaccine assembly from surface proteins of Staphylococcus aureus.
Staphylococcus aureus is the most common cause of hospital-acquired infection. Because of the emergence of antibiotic-resistant strains, these infections represent a serious public health threat. To develop a broadly protective vaccine, we tested cell wall-anchored surface proteins of S. aureus as antigens in a murine model of abscess formation. Immunization with four antigens (IsdA, IsdB, SdrD, and SdrE) generated significant protective immunity that correlated with the induction of opsonophagocytic antibodies. When assembled into a combined vaccine, the four surface proteins afforded high levels of protection against invasive disease or lethal challenge with human clinical S. aureus isolates.
Stranger-Jones
Yukiko K
YK
Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA.
Bae
Taeok
T
Schneewind
Olaf
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D000038:Abscess / Q000517:prevention & control; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D000954:Antigens, Surface / Q000008:administration & dosage / Q000235:genetics; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D027682:Cation Transport Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013203:Staphylococcal Infections / Q000276:immunology / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D017778:Vaccines, Combined / Q000008:administration & dosage / Q000235:genetics
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002135:Calcium-Binding Proteins; D027682:Cation Transport Proteins; C512791:IsdA protein, Staphylococcus aureus; C510010:IsdB protein, Staphylococcus aureus; D008565:Membrane Proteins; C115915:SdrD protein, Staphylococcus aureus; C118507:SdrE protein, Staphylococcus aureus; D017778:Vaccines, Combined