[Studies on HPLC fingerprint of Radix Aconiti Kusnezoffii].
OBJECTIVE
To establish the HPLC fingerprint of Radix Aconiti Kusnezoffii.


METHODS
The chromatographic separation was performed on a Kromasil C18 (4.6 mm x 250 mm, 5 microm) eluted with a mobile phase of acetonitrile-ammonium acetate buffer (2. 5 per thousand acetic acid-ammonia pH 10.5) (60:40). The UV detection wavelength was set at 240 nm and the flow rate was set at 1.0 mL x min(-1).


RESULTS
The RSD of precision and repeatability was less than 2%. Under the selected chromatographic conditions, good HPLC fingerprints of Radix Aconiti Kusnezoffii were obtained.


CONCLUSIONS
The method was simple, accurate and repeatable. It can be used for the quality control of Radix Aconiti Kusnezoffii.
Zhao
Ying-yong
YY
Wenshan Prefecture Sanqi Science and Technology Research Institute, China.
Cui
Xiu-ming
XM
Dai
Yun
Y
Miao
Hua
H
D000157:Aconitine / Q000031:analogs & derivatives* / Q000032:analysis; D031841:Aconitum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
C019470:mesaconitine; D000157:Aconitine