Quantitative analysis of trace chromium in blood samples. Combination of the advanced oxidation process with catalytic adsorptive stripping voltammetry.
A new method for pretreating blood samples for trace Cr analysis is described. The advanced oxidation process (AOP with H2O2 and 5.5-W UV irradiation for 60 min) is used to remove biological/organic species for subsequent analysis. Prior to the AOP pretreatment, acid (HNO3) is used at pH 3.0 to inhibit the enzyme catalase in the blood samples. Catalytic adsorptive stripping voltammetry at a bismuth film electrode gives a Cr concentration of 6.0 +/- 0.3 ppb in the blood samples. This concentration was confirmed by dry-ashing the blood samples and subsequent analysis by atomic absorption spectroscopy. This current method may be used to monitor chromium, a trace metal in humans, and the efficacy and safety of chromium supplements as adjuvant therapy for diabetes.
Yong
Li
L
Department of Chemistry, The University of Tennessee, Knoxville, Tennessee 37996-1600, USA.
Armstrong
Kristie C
KC
Dansby-Sparks
Royce N
RN
Carrington
Nathan A
NA
Chambers
James Q
JQ
Xue
Zi-Ling
ZL
D000327:Adsorption; D002138:Calibration; D002384:Catalysis; D002857:Chromium / Q000097:blood*; D004563:Electrochemistry / Q000379:methods*; D006861:Hydrogen Peroxide; D010084:Oxidation-Reduction
D002857:Chromium; D006861:Hydrogen Peroxide