[Preliminary studies on physicochemical properties of Angelica sinensis polysaccharide-iron complex].
OBJECTIVE
To study some physicochemical properties of Angelica sinensis polysaccharide-iron complex (APC).


METHODS
Based on the qualitatively identified reactions of iron (III), the qualitatively identified reactions of APC were found out by comparing hydroxide. The content of iron (III) in APC was determined with iodometry. The stability of APC under physiological pH conditions was judged by titrating APC with sodium hydroxide. The deoxidization of APC was tested with colorimetric analysis.


RESULTS
APC showed the qualitatively identified reactions of iron (III). The content of iron (III) in APC ranged from 10% to 40%, and the water-solubility of APC was related to the content of iron (III). The complex was stable at physiological pH from 3 to 12, without precipitation and dissociation. At 37 degrees C, Fe (III) in the complex was completely reduced to Fe (III) by ascorbic acid in about 6 hours.


CONCLUSIONS
APC can be qualitatively identified by using the qualitatively identified reactions of iron (III). When its iron (III) content is within 20%-25%, APC has a better ability to dissolve in water. And the complex is stable under physiological pH conditions.
Wang
Kai-ping
KP
Faculty of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. wkpzcq@163.com
Zhang
Yu
Y
Dai
Li-quan
LQ
D029971:Angelica sinensis / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004355:Drug Stability; D005290:Ferric Compounds / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage* / Q000302:isolation & purification; D012995:Solubility
D005290:Ferric Compounds; D011134:Polysaccharides; D007501:Iron