Transfer of apatite coating from porogens to scaffolds: uniform apatite coating within porous poly(DL-lactic-co-glycolic acid) scaffold in vitro.
Strategies to bone tissue engineering have focused on the use of synthetic or natural degradable materials as scaffolds for cell transplantation to guide bone regeneration. Biocompatibility, biodegradability, biomechanical integrity, and osteoconductivity are important requirements for the scaffold materials. This study explored a new approach of apatite coating to enhance the osteoconductivity of a synthetic degradable poly(DL-lactic-co-glycolic acid) (PLGA) scaffold. The new approach was developed to ensure a relatively uniform apatite coating on the interior pore surfaces deep inside a scaffold, even for a relatively thick scaffold with small pores. Apatite was first coated on the surface of paraffin spheres of the desirable sizes. The paraffin spheres were then molded to form a foam. PLGA/pyridine solution was cast into the interspaces among the paraffin spheres. After the paraffin spheres were dissolved and removed by cyclohexane, PLGA scaffold with controlled pore size, good interconnectivity and high porosity was obtained with apatite left on the pore surface uniformly throughout the whole scaffold. The scaffold and apatite coating were characterized using thermogravimetry analysis, scanning electron microscopy, energy dispersive X-ray spectroscopy, and X-ray diffractometry.
Li
Jiashen
J
Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Hum, Kowloon, Hong Kong, China.
Beaussart
Audrey
A
Chen
Yun
Y
Mak
Arthur F T
AF
D001861:Bone Regeneration; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D023822:Tissue Engineering
D020099:Coated Materials, Biocompatible; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid