title: Compounds with DNA cleaving activity from Kadsura ananosma.
abstract: Two DNA cleavage agents, meso-dihydroguaiaretic acid (1) and isobavachalcone (2) together with the known alpha-ylangene, beta-sitosterol, daucosterol, pentacosane, hexacosanic acid and cerotic acid 1-monoglyceride were isolated from the stem barks of Kadsura ananosma Kerr for the first time. Compounds 1 and 2 showed relaxation of supercoiled DNA to nicked DNA. 1 represented a new structural type of DNA cleavage agent, while 2 was reported to show DNA strand-scission activity for the first time. 1 also showed significant cytotoxic effect on Hela and Leukemia cells in vitro.
authors.affiliation: Department of Chemistry, Yunnan Normal University, China. ygchen48@hotmail.com
authors.forename: Ye-Gao
authors.initials: YG
authors.lastname: Chen
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Song
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Hai
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Lv
authors.forename: A
authors.initials: A
authors.lastname: Fang
authors.forename: F
authors.initials: F
authors.lastname: Halaweish
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Liao
mesh_terms: D045744:Cell Line, Tumor; D047188:Chalcones / Q000737:chemistry* / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004278:DNA, Superhelical / Q000737:chemistry / Q000187:drug effects; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D032135:Kadsura / Q000737:chemistry*; D007938:Leukemia / Q000188:drug therapy / Q000473:pathology; D017705:Lignans / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009690:Nucleic Acid Conformation; D024301:Plant Bark / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
chemical_list: D047188:Chalcones; D004278:DNA, Superhelical; D017705:Lignans; C468754:isobavachalcone; C015816:dihydroguaiaretic acid; D006139:Guaiacol; D004247:DNA