Pilot study of the porcine uterine horn as an in vivo appendicitis model for development of endoscopic transgastric appendectomy.
BACKGROUND
Iatrogenic inflammation of the porcine uterine horn may serve as an in vivo appendicitis model for the development of endoscopic transgastric appendectomy.


OBJECTIVE
Five female pigs.


METHODS
Animal study.


METHODS
General anesthesia.


METHODS
Anatomical appearance and technical feasibility.


METHODS
Three pigs were used to identify an injectable material that would inflame the uterine horn, and 2 pigs were used for a pilot appendectomy. Three types of materials were individually injected into the bilateral uterine horns, and the ideal material to inflame the uterine horn was injected into the right uterine horn for the last 2 pigs. After 24 hours, the injected uterine horns of the first 3 pigs were assessed and a pilot appendectomy was performed in the last 2 pigs.


RESULTS
Ethanolamine oleate (EO) injected uterine horns demonstrated similarities to the inflamed human appendix. Simulations of the appendectomy were successfully performed by using the EO model.


CONCLUSIONS
Suboptimal existing tools.


CONCLUSIONS
This preliminary study demonstrated the technical feasibility to create a model for acute appendicitis by using the porcine uterine horn and transgastric appendectomy.
Sumiyama
Kazuki
K
Developmental Endoscopy Unit, Charlton 8-200, Mayo Clinic, 200 First St SW, Rochester, MN 55905, USA.
Gostout
Christopher J
CJ
Rajan
Elizabeth
E
Bakken
Timothy A
TA
Deters
Jodie L
JL
Knipschield
Mary A
MA
Hawes
Robert H
RH
Kalloo
Anthony N
AN
Pasricha
Pankaj J
PJ
Chung
Sydney
S
Kantsevoy
Sergey V
SV
Cotton
Peter B
PB
D000818:Animals; D001062:Appendectomy / Q000379:methods; D001064:Appendicitis / Q000601:surgery*; D004195:Disease Models, Animal; D004716:Endometritis / Q000139:chemically induced / Q000601:surgery; D016145:Endoscopy, Digestive System; D005240:Feasibility Studies; D005260:Female; D005774:Gastrostomy; D009829:Oleic Acids / Q000009:adverse effects; D010865:Pilot Projects; D012107:Research Design; D012597:Sclerosing Solutions / Q000009:adverse effects; D013552:Swine; D014599:Uterus / Q000473:pathology / Q000601:surgery*
D009829:Oleic Acids; D012597:Sclerosing Solutions; C033343:ethanolamine oleate