title: [Multiple intracerebral lesions. Identification of the causative agent by 16S rDNA-PCR].
abstract: Molecular biological methods such as polymerase chain reaction (PCR) enable microbiologists to detect bacteria even if antibiotic treatment has already been started. Based on this case report of a 39-year-old man with multiple intracerebral lesions, we describe a PCR method called 16S rDNA-PCR which can be used to identify panbacterial DNA by focussing on the universal gene sequences for the bacterial 16S part of the ribosome.
authors.affiliation: Institut für Medizinische Mikrobiologie, Klinikum der Friedrich-Schiller-Universität, Semmelweisstrasse 4, 07743 Jena, Germany. KATHARINA.Boden@med.uni-jena.de
authors.forename: K
authors.initials: K
authors.lastname: Boden
authors.forename: F
authors.initials: F
authors.lastname: Joachimski
authors.forename: C
authors.initials: C
authors.lastname: Ewald
authors.forename: W
authors.initials: W
authors.lastname: Behrendt
authors.forename: M
authors.initials: M
authors.lastname: Baier
authors.forename: K-H
authors.initials: KH
authors.lastname: Schmidt
authors.forename: E
authors.initials: E
authors.lastname: Straube
mesh_terms: D000328:Adult; D001921:Brain / Q000473:pathology; D001922:Brain Abscess / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal; D003937:Diagnosis, Differential; D005674:Fusobacterium Infections / Q000175:diagnosis* / Q000382:microbiology; D016967:Fusobacterium nucleatum / Q000235:genetics*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S