Albumin is a redox-active crowding agent that promotes oxidative folding of cysteine-rich peptides.
Oxidative folding that occurs in a crowded cellular milieu is characterized by multifaceted interactions that occur among nascent polypeptides and resident components of the endoplasmic reticulum (ER) lumen. Macromolecular crowding has been considered an essential factor in the folding of polypeptides, but the excluded volume effect has not been evaluated for small, disulfide-rich peptides. In the research presented, we examined how macromolecular crowding agents, such as albumin, ovalbumin, and polysaccharides, influenced the kinetics and thermodynamics of forming disulfide bonds in four model peptides of varying molecular size from 13 residues (1.4 kDa) to 58-residues (6.5 kDa): conotoxins: GI, PVIIA, r11a, and bovine pancreatic trypsin inhibitor. Our results indicate that the excluded volume effect does not significantly alter the folding rates nor equilibria for these peptides. In stark contrast, folding reactions were dramatically accelerated, when protein-based crowding agents were present at concentrations lower than those predicted to provide the excluded volume effect. Submillimolar albumin alone was as effective as glutathione in promoting the oxidative folding of GI conotoxin at concentrations typically found in the ER. To the best of our knowledge, this is the first report and quantitative characterization of oxidative folding of peptides mediated by other than thioredoxin-based protein disulfide bonds. Our work raises a possibility that concurrent secretory and ER-resident proteins may influence the oxidative folding of small, cysteine-rich peptides not as crowding agents, but as redox-active factors.
Buczek
Olga
O
Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.
Green
Brad R
BR
Bulaj
Grzegorz
G
D000418:Albumins / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D007611:Aprotinin / Q000737:chemistry / Q000187:drug effects / Q000235:genetics; D001704:Biopolymers / Q000737:chemistry; D002417:Cattle; D002645:Chickens; D020916:Conotoxins / Q000737:chemistry / Q000235:genetics; D003545:Cysteine / Q000737:chemistry; D003911:Dextrans / Q000494:pharmacology; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000494:pharmacology; D010084:Oxidation-Reduction; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013816:Thermodynamics
D000418:Albumins; D001704:Biopolymers; D020916:Conotoxins; D003911:Dextrans; D046911:Macromolecular Substances; D011506:Proteins; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D007611:Aprotinin; D003545:Cysteine