Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide.
Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria.
Marolda
Cristina L
CL
Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada.
Lahiry
Piya
P
Vinés
Enrique
E
Saldías
Soledad
S
Valvano
Miguel A
MA
D001431:Bacteriological Techniques / Q000379:methods*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005998:Glycine / Q000031:analogs & derivatives / Q000737:chemistry; D008050:Lipid A / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis / Q000737:chemistry; D015336:Molecular Probe Techniques; D019081:O Antigens / Q000032:analysis*; D016622:Silver Staining; D013400:Sugar Acids / Q000032:analysis / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry
D008050:Lipid A; D008070:Lipopolysaccharides; D019081:O Antigens; D013400:Sugar Acids; D013438:Sulfhydryl Compounds; C002532:2-keto-3-deoxyoctonate; C006537:4-amino-5-hydrazino-1,2,4-triazole-3-thiol; D005998:Glycine; C100184:tricine