title: Effective immobilization of subunit protein in mesoporous silica modified with ethanol.
abstract: Ethoxylated FSM-type mesoporous silica (folded-sheet mesoporous material) with a pore diameter of 6.2 nm (FSM6.2) remarkably enhances rigidly of the structure in aqueous solutions. The esterified material could be used successfully as an adsorbent to accommodate subunit protein, methemoglobin (Fe(3+)). Furthermore, methemoglobin (Fe(3+)) in the pores of ethoxy-FSM is maintained a peroxidase activity similar to the native, indicating methemoglobin retains its fore subunit structure in the pores of FSM6.2.
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Nigatake 4-2-1, Miyagino-ku, Sendai 983-8551, Japan. t-itoh@ni.aist.go.jp
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Itoh
authors.forename: Ryo
authors.initials: R
authors.lastname: Ishii
authors.forename: Takeo
authors.initials: T
authors.lastname: Ebina
authors.forename: Takaaki
authors.initials: T
authors.lastname: Hanaoka
authors.forename: Takuji
authors.initials: T
authors.lastname: Ikeda
authors.forename: Yoko
authors.initials: Y
authors.lastname: Urabe
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Fukushima
authors.forename: Fujio
authors.initials: F
authors.lastname: Mizukami
mesh_terms: D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry*; D008422:Materials Testing; D008706:Methemoglobin / Q000737:chemistry*; D016062:Porosity; D011485:Protein Binding; D021122:Protein Subunits; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D021122:Protein Subunits; D014867:Water; D000431:Ethanol; D012822:Silicon Dioxide; D008706:Methemoglobin