title: Rhythmic expression of the cycle gene in a hematophagous insect vector.
abstract: BACKGROUND
  A large number of organisms have internal circadian clocks that enable them to adapt to the cyclic changes of the external environment. In the model organism Drosophila melanogaster, feedback loops of transcription and translation are believed to be crucial for the maintenance of the central pacemaker. In this mechanism the cycle (or bmal1) gene, which is constitutively expressed, plays a critical role activating the expression of genes that will later inhibit their own activity, thereby closing the loop. Unlike Drosophila, the molecular clock of insect vectors is poorly understood, despite the importance of circadian behavior in the dynamic of disease transmission.
  
  
  RESULTS
  Here we describe the sequence, genomic organization and circadian expression of cycle in the crepuscular/nocturnal hematophagous sandfly Lutzomyia longipalpis, the main vector of visceral leishmaniasis in the Americas. Deduced amino acid sequence revealed that sandfly cycle has a C-terminal transactivation domain highly conserved among eukaryotes but absent in D. melanogaster. Moreover, an alternative form of the transcript was also identified. Interestingly, while cycle expression in Drosophila and other Diptera is constitutive, in sandflies it is rhythmic in males and female heads but constitutive in the female body. Blood-feeding, which causes down-regulation of period and timeless in this species, does not affect cycle expression.
  
  
  CONCLUSIONS
  Sequence and expression analysis of cycle in L. longipalpis show interesting differences compared to Drosophila suggesting that hematophagous vector species might present interesting new models to study the molecular control of insect circadian clocks.
authors.affiliation: Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz--Fiocruz, Rio de Janeiro 21045-900, Brazil. acamf@ioc.fiocruz.br <acamf@ioc.fiocruz.br>
authors.forename: Antonio C A
authors.initials: AC
authors.lastname: Meireles-Filho
authors.forename: Paulo R
authors.initials: PR
authors.lastname: Amoretty
authors.forename: Nataly A
authors.initials: NA
authors.lastname: Souza
authors.forename: Charalambos P
authors.initials: CP
authors.lastname: Kyriacou
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Peixoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D012831:Bombyx / Q000235:genetics; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D017344:Genes, Insect / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007303:Insect Vectors / Q000235:genetics; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010802:Phylogeny; D011576:Psychodidae / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D012333:RNA, Messenger; D015534:Trans-Activators