MassTag polymerase-chain-reaction detection of respiratory pathogens, including a new rhinovirus genotype, that caused influenza-like illness in New York State during 2004-2005.
In New York State during winter 2004, there was a high incidence of influenza-like illness that tested negative both for influenza virus, by molecular methods, and for other respiratory viruses, by virus culture. Concern that a novel pathogen might be implicated led us to implement a new multiplex diagnostic tool. MassTag polymerase chain reaction resolved 26 of 79 previously negative samples, revealing the presence of rhinoviruses in a large proportion of samples, half of which belonged to a previously uncharacterized genetic clade. In some instances, knowledge of the detected viral and/or bacterial (co)infection could have altered clinical management.
Lamson
Daryl
D
Clinical Virology Program, Wadsworth Center, New York State Department of Health, Albany, NY, USA.
Renwick
Neil
N
Kapoor
Vishal
V
Liu
Zhiqiang
Z
Palacios
Gustavo
G
Ju
Jingyue
J
Dean
Amy
A
St George
Kirsten
K
Briese
Thomas
T
Lipkin
W Ian
WI
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009518:New York / Q000453:epidemiology; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000453:epidemiology / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000382:microbiology* / Q000821:virology*; D012229:Rhinovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics; D014780:Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D014764:Viral Proteins