title: [Inhibition effect of active fraction from Actinidia valvata on growth of transplanated mouse tumor cells and preliminary study of its mechanism].
abstract: OBJECTIVE
  To study the inhibition effect of active fraction from maorenshen (MA, MB) on the growth of transplanted mouse tumor cells H22 and its mechanism.
  
  
  METHODS
  The transplanted mouse tumor model in vivo was used to observe the antitumor activity of MA, MB. The cell cycle distributions were determined by flow cytometry (FCM). The apoptosis in tumor was measured by TdT-mediated biotinyated-dUTP nick end-labeling (TUNEL) method.
  
  
  RESULTS
  MA, MB showed antitumor activitiy on transplanted mouse tumor. They could make cells arrested at G0-G1 phase, decrease cells percentage at S phase, and induce their apoptosis.
  
  
  CONCLUSIONS
  MA, MB from maorenshen have anti-tumor effect on the transplanted mouse tumor, and the mechanism may be through the way of influencing cell cycle and inducing apoptosis.
authors.affiliation: Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Ya-ni
authors.initials: YN
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Chang-quan
authors.initials: CQ
authors.lastname: Ling
mesh_terms: D029042:Actinidia / Q000737:chemistry; D000818:Animals; D000880:Anthraquinones / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000880:Anthraquinones; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D012503:Saponins