Transcriptional expression of calvarial bone after treatment with low-intensity ultrasound: an in vitro study.
The capacity to reossify a calvarial bone defect is very small in mature animals and in humans greater than 2 y of age. The clinical treatment of injured tissue sites of bones by low-intensity pulsed ultrasound is widespread, but little is known about the precise effects of ultrasound on the fundamental processes that promote repair and regeneration. In this study, we used real-time polymerase chain reaction (RT-PCR) to investigate the expression of osteogenesis-associated genes after stimulation by low-intensity ultrasound in adult mouse osteoblast from the parietal calvaria. The gene associated with the Runx2 pathway had notably higher levels after 1, 2 and 3 days of stimulation. Therefore, low-intensity ultrasound seems to have an effect on the transcriptional gene expression of the calvarial bone in vitro.
Gleizal
Arnaud
A
Department of Oral and Maxillofacial Surgery, Hôpitaux Nord, Lyon, France. jean-luc.beziat@chu-lyon.fr
Li
Shuli
S
Pialat
Jean-Baptiste
JB
Beziat
Jean-Luc
JL
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D008565:Membrane Proteins; D051379:Mice; D010006:Osteoblasts; D010012:Osteogenesis / Q000235:genetics*; D010294:Parietal Bone / Q000166:cytology*; D016133:Polymerase Chain Reaction / Q000379:methods; D014158:Transcription, Genetic / Q000235:genetics; D014465:Ultrasonics
D048868:Adaptor Proteins, Signal Transducing; C480020:Atrnl1 protein, mouse; D019485:Bone Morphogenetic Proteins; D050659:Core Binding Factor Alpha 1 Subunit; D008565:Membrane Proteins; C493716:Runx2 protein, mouse