Improving endothelial healing with novel chimeric mitogens.
BACKGROUND
Chimeric proteins may be used to direct cell-specific activity. Heparin-binding growth-associated molecule (HBGAM) binds to cell receptors that are relatively more robust on endothelial cells, and it may confer endothelial cell selectivity to potent angiogens such as fibroblast growth factor-1 (FGF-1).


METHODS
By ligating fibroblast growth factor or its potent mutant, S130K, to HBGAM, we tested their effect on re-endothelialization after angioplasty injury by using a canine model.


RESULTS
Both HBGAM/S130K- and HBGAM/FGF-1-treated arteries had increased neointimal mitotic index and re-endothelialization rates at 30 days compared with control arteries without inducing a significant increase in the neointimal thickness or the ratio of neointimal to medial thickness between treatment and control groups.


CONCLUSIONS
HBGAM/S130K and HBGAM/FGF-1 facilitates endothelial healing without myointimal thickening after canine carotid artery balloon angioplasty injury. Application of these growth factors in fibrin glue may improve endothelialization clinically after angioplasty or endarterectomy.
Brewster
Luke
L
Department of Surgery, Loyola University, 2160 South First Avenue, Maywood, IL 60153, USA.
Brey
Eric M
EM
Addis
Michael
M
Xue
Lian
L
Husak
Vicki
V
Ellinger
Joan
J
Haudenschild
Christian C
CC
Greisler
Howard P
HP
D000818:Animals; D020212:Carotid Artery Injuries / Q000188:drug therapy*; D002352:Carrier Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D016207:Cytokines / Q000494:pharmacology / Q000627:therapeutic use*; D004285:Dogs; D004730:Endothelium, Vascular / Q000187:drug effects / Q000293:injuries*; D016220:Fibroblast Growth Factor 1; D006133:Growth Substances / Q000627:therapeutic use*; D008855:Microscopy, Electron, Scanning; D008940:Mitotic Index; D023421:Models, Animal; D050506:Mutant Chimeric Proteins; D011993:Recombinant Fusion Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D017539:Tunica Intima / Q000473:pathology; D017540:Tunica Media / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
D002352:Carrier Proteins; D016207:Cytokines; D006133:Growth Substances; D050506:Mutant Chimeric Proteins; D011993:Recombinant Fusion Proteins; C494171:S130K-HBGAM chimeric protein; D016220:Fibroblast Growth Factor 1; C066845:pleiotrophin