title: Tumor necrosis factor-alpha promoter polymorphisms in Mexican patients with spondyloarthritis.
abstract: To evaluate the role of tumor necrosis factor-alpha (TNF-alpha) gene as susceptibility marker for spondyloarthritis (SpA), two polymorphisms (-238 and -308 positions) were analyzed in 229 patients with SpA (113 with ankylosing spondylitis [AS], 92 with undifferentiated SpA [U-SpA], 24 with reactive arthritis), and 169 ethnically matched healthy control subjects. The HLA-B alleles were detected by PCR-SSP technique and the TNF-alpha polymorphism by PCR-RFLP. In comparison with healthy control subjects, the frequencies of TNF-238 in SpA were similar. In contrast, the analysis of -308 polymorphism showed increased frequencies of the T2(A) allele in the whole SpA group (p < 0.05, pC = NS, OR = 1.83) as well as the T2(A) allele (pC < 0.05, OR = 2.4) and T1T2(AG) genotype (p < 0.05, pC = NS, OR = 2.25) in U-SpA patients. Comparison of B27-negative patients and healthy control subjects yielded similar results. There was no significant correlation between TNF genotypes and clinical data. The present study demonstrates that TNF-alpha -308 polymorphism appears to be associated with the genetic susceptibility U-SpA. The association seems independent of the susceptibility conferred by the HLA-B27 in this group of patients.
authors.affiliation: Department of Physiology, Instituto Nacional de Cardíología Ignacio Chávez, Mexico City, Mexico. gvargas63@yahoo.com
authors.forename: Gilberto
authors.initials: G
authors.lastname: Vargas-Alarcón
authors.forename: Julio
authors.initials: J
authors.lastname: Casasola-Vargas
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Rodríguez-Pérez
authors.forename: Gabriela
authors.initials: G
authors.lastname: Huerta-Sil
authors.forename: Nonanzit
authors.initials: N
authors.lastname: Pérez-Hernández
authors.forename: John
authors.initials: J
authors.lastname: Londoño
authors.forename: Cesar
authors.initials: C
authors.lastname: Pacheco-Tena
authors.forename: Mario H
authors.initials: MH
authors.lastname: Cardiel
authors.forename: Julio
authors.initials: J
authors.lastname: Granados
authors.forename: Rubén
authors.initials: R
authors.lastname: Burgos-Vargas
mesh_terms: D000293:Adolescent; D000328:Adult; D016918:Arthritis, Reactive / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D015796:HLA-B27 Antigen / Q000235:genetics; D006801:Humans; D008297:Male; D008800:Mexico; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D025241:Spondylarthritis / Q000235:genetics*; D013167:Spondylitis, Ankylosing / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D015796:HLA-B27 Antigen; D014409:Tumor Necrosis Factor-alpha