[Determination of danshensu and protocatechuic aldehyde in Guanxinning injection powder by RP-HPLC].
OBJECTIVE
An RP-HPLC procedure was established for the determination of danshensu and protocatechuic aldehyde in Guanxinning injection powder.


METHODS
An RP-HPLC analytical procedure was developed using Hypersil ODS2 C18 column (4.6 mm x 250 mm, 5 microm) with methanol-0.5% glaceal acetic acid (4.5:95.5) as mobile phase, and a wavelength of 280 nm for UV detection.


RESULTS
The linear range was 3.004-45.06 microg x m(L-1) (r = 0.999 5, n = 6) for danshensu, and 0.300-4.509 microg x mL(-1) (r = 0.999 3, n = 6) for protocatechuic aldehyde. The average recoveries were 99.1% and 97.9%, respectively.


CONCLUSIONS
The method was stable, accurate, and reproducible, and can be used for the quality control of Guanxinning injection powder.
Guo
Xiao-Rui
XR
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Zhang
Wen-Ting
WT
Chen
Xiao-Hui
XH
Bi
Kai-Shun
KS
D001547:Benzaldehydes / Q000032:analysis*; D002396:Catechols / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D007267:Injections; D007773:Lactates / Q000032:analysis*; D030004:Ligusticum / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011208:Powders; D011786:Quality Control; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry
D001547:Benzaldehydes; D002396:Catechols; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007773:Lactates; D011208:Powders; C035055:3,4-dihydroxyphenyllactic acid; C005581:protocatechualdehyde