[Construction and identification of tetracycline-inducible human hepatocyte growth factor eukaryotic expression vector].
OBJECTIVE
To construct a tetracycline-inducible eukaryotic expression vector containing human hepatocyte growth factor (HGF) cDNA.


METHODS
Human HGF cDNA fragment was obtained by PCR from pUC-SRalpha/HGF plasmid and inserted into the restriction site between Mlu I and Sal I of the tetracycline-inducible eukaryotic expression vector pBI-L. pBI-L-HGF was constructed by DNA recombination in vitro, and was identified by restriction endonucleases digestion and sequencing.


RESULTS
The fragment of pBI-L-HGF digested with restriction endonucleases well corresponded to expectation, and the sequence of inserted HGF cDNA was correct according to the GenBank.


CONCLUSIONS
The tetracycline-inducible eukaryotic expression vector of human HGF pBI-L-HGF has been constructed successfully, which allows further study of HGF gene therapy with much safety and easy manipulation.
Ren
Shu-Ting
ST
Department of Pathology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China. rst@mail.xjtu.edu.cn
Yu
Lin-hua
LH
Xu
Chang-fu
CF
Gao
Guang-dao
GD
D018076:DNA, Complementary / Q000235:genetics; D005057:Eukaryotic Cells / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D005822:Genetic Vectors / Q000235:genetics*; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D013752:Tetracycline / Q000494:pharmacology*
D018076:DNA, Complementary; D017228:Hepatocyte Growth Factor; D013752:Tetracycline