Effect of a fluvalinate-resistance-associated sodium channel mutation from varroa mites on cockroach sodium channel sensitivity to fluvalinate, a pyrethroid insecticide.
Fluvalinate is a pyrethroid insecticide that is widely used in the control of the varroa mite (Varroa destructor), an ecto-parasite of the honeybee. Previously we identified four fluvalinate-resistance-associated mutations in the sodium channel gene of the varroa mite. One of the mutations caused a leucine (L) to proline (P) change at 1770 in the linker connecting domains III and IV of the sodium channel. Interestingly, at the position corresponding to the L to P mutation, all known insect (including honeybee) sodium channel proteins already naturally contain a P residue (e.g., P1577 in the cockroach sodium channel BgNa(v)). To determine whether insect sodium channels are less sensitive to fluvalinate than arachnid sodium channels, we replaced P1577 with an L in a BgNa(v) variant (BgNa(v)1-1) and examined the sensitivity of the recombinant channel to fluvalinate. The P1577L substitution did not alter the gating properties of the BgNa(v)1-1 channel expressed in Xenopus oocytes. However, the BgNa(v)1-1(P1577L) channel was five-fold more sensitive to fluvalinate compared with the BgNa(v)1-1 channel. These results not only implicate the L to P mutation in fluvalinate resistance in varroa mites, but also suggest a possible contribution of L1770 to the higher sensitivity of varroa mites to fluvalinate than their insect hosts.
Liu
Zhiqi
Z
Department of Entomology, Michigan State University, East Lansing, MI 48824, USA.
Tan
Jianguo
J
Huang
Zachary Y
ZY
Dong
Ke
K
D000818:Animals; D020048:Blattellidae / Q000235:genetics / Q000502:physiology*; D007305:Insecticide Resistance / Q000235:genetics / Q000502:physiology; D007306:Insecticides; D008925:Mites / Q000235:genetics / Q000502:physiology*; D020125:Mutation, Missense; D009570:Nitriles; D011722:Pyrethrins; D015222:Sodium Channels / Q000235:genetics / Q000502:physiology*
D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D015222:Sodium Channels; C034227:fluvalinate