Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain.
The ESCRT-I and ESCRT-II complexes help sort ubiquitinated proteins into vesicles that accumulate within multivesicular bodies (MVBs). Crystallographic and biochemical analyses reveal that the GLUE domain of the human ESCRT-II EAP45 (also called VPS36) subunit is a split pleckstrin-homology domain that binds ubiquitin along one edge of the beta-sandwich. The structure suggests how human ESCRT-II can couple recognition of ubiquitinated cargoes and endosomal phospholipids during MVB protein sorting.
Alam
Steven L
SL
Department of Biochemistry, University of Utah, Salt Lake City, Utah 84112-5650, USA.
Langelier
Charles
C
Whitby
Frank G
FG
Koirala
Sajjan
S
Robinson
Howard
H
Hill
Christopher P
CP
Sundquist
Wesley I
WI
D020816:Amino Acid Motifs; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray; D056827:Endosomal Sorting Complexes Required for Transport; D011992:Endosomes / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D022161:Transport Vesicles / Q000378:metabolism*; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000737:chemistry / Q000378:metabolism
D002352:Carrier Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D011993:Recombinant Fusion Proteins; D025801:Ubiquitin; C516152:VPS36 protein, human; D033921:Vesicular Transport Proteins