title: Local and systemic interactions related to serum transforming growth factor-beta levels in burn wounds of various depths.
abstract: OBJECTIVE
  This study aimed to investigate the interaction between serum levels of TGF-beta and active-immune cell infiltration in burn wounds of various depths.
  
  
  METHODS
  Thirty female Sprague-Dawley rats were divided into three groups: full-thickness burns (F), partial-thickness burns (P), and no burns (S). After burn-induction, blood samples were obtained only once from shams and at postburn 1, 48 h, and 7 days in burn groups. Serum levels of TGF-beta were measured by means of the ELISA. The proportions of neutrophils, fibroblasts, vascular proliferation, CD68-macrophages, and CD3-lymphocytes were studied immunohistochemically and graded semiquantitatively.
  
  
  RESULTS
  Serum TGF-beta levels in the F and P groups were lower than those in sham at 1h after burn (p<.05). No significant differences in TGF-beta were observed between groups F and P on days 2 and 7 after injury. No local accumulation of macrophages and fibroblasts was noted in either burn group, but the proportion of lymphocytes was higher in the P group at 1h after burn. Neutrophils were higher in the F group than the P on day 7 after burn.
  
  
  CONCLUSIONS
  Prolonged neutrophil infiltration in full-thickness burn wounds and suppressed lymphocyte proliferation in partial-thickness burn wounds seem to be related to an increase in serum TGF-beta levels.
authors.affiliation: Başkent University, Burn and Fire Disaster Institute, Ankara, Turkey.
authors.forename: A Ebru
authors.initials: AE
authors.lastname: Sakallioğlu
authors.forename: Ozgür
authors.initials: O
authors.lastname: Başaran
authors.forename: B Handan
authors.initials: BH
authors.lastname: Ozdemir
authors.forename: Zübeyde
authors.initials: Z
authors.lastname: Arat
authors.forename: Muammer
authors.initials: M
authors.lastname: Yücel
authors.forename: Mehmet
authors.initials: M
authors.lastname: Haberal
mesh_terms: D000818:Animals; D002056:Burns / Q000097:blood* / Q000276:immunology / Q000473:pathology; D049109:Cell Proliferation; D005260:Female; D005347:Fibroblasts / Q000276:immunology; D006684:HLA-DR Antigens / Q000378:metabolism; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D020556:Neutrophil Infiltration / Q000276:immunology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D006684:HLA-DR Antigens; D016212:Transforming Growth Factor beta