[Study on supension culture and quantitative determination of total ginsenosides from the crown gall of Panax quinquefolium].
The influences of different cultural node on the crown gall culture of Panax quinquefolium and its ginsenosides contents were studied. The faster growth rate was obtained in suspension culture. And the dry weight (DW) of crown gall and the accumulation of total ginsenoside content reached 0. 776 g DW/flask and 19.4 mg/flask, which were 111% and 64% higher than solid culture, respectively. The results also indicated that the utilization (by percentage) of sucrose was 91.8%. The investigation provided useful information for following researches on fermentation culture and large-scale production of ginsenosides.
Yu
Rong-min
RM
Jinan University College of Pharmacy, Guangzhou, China.
Jin
Qian-xing
QX
Zhao
Yu
Y
D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D036145:Ginsenosides / Q000096:biosynthesis* / Q000302:isolation & purification; D005894:Panax / Q000254:growth & development* / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D013535:Suspensions; D013997:Time Factors
D003470:Culture Media; D036145:Ginsenosides; D013535:Suspensions; D002244:Carbon