title: Genetic architecture of Hsp90-dependent drug resistance.
abstract: Hsp90 potentiates the evolution of azole resistance in the model yeast Saccharomyces cerevisiae and the opportunistic pathogen Candida albicans via calcineurin. Here, we explored effectors downstream of calcineurin regulating this Hsp90-dependent trait. Using S. cerevisiae erg3 mutants as a model, we determined that both Crz1 and Hph1 modulate azole resistance.
authors.affiliation: Whitehead Institute, Nine Cambridge Center, Cambridge, MA 02142, USA.
authors.forename: Leah E
authors.initials: LE
authors.lastname: Cowen
authors.forename: Anne E
authors.initials: AE
authors.lastname: Carpenter
authors.forename: Oranart
authors.initials: O
authors.lastname: Matangkasombut
authors.forename: Gerald R
authors.initials: GR
authors.lastname: Fink
authors.forename: Susan
authors.initials: S
authors.lastname: Lindquist
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D019703:Calcineurin / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000235:genetics; D015725:Fluconazole / Q000494:pharmacology; D005800:Genes, Fungal; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; C074510:HSP82 protein, S cerevisiae; D018841:HSP90 Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; D015725:Fluconazole; D010088:Oxidoreductases; C039306:sterol delta-5 desaturase; D019703:Calcineurin