title: Prediction of measurement uncertainty in isotope dilution gas chromatography/mass spectrometry.
abstract: An equation is theoretically derived which describes the relative standard deviation (RSD) of the amount ratios of analyte to its isotope-labeled variant in gas chromatography/mass spectrometry (GC/MS) using the stable isotope dilution method. The determination of methyltestosterone is taken as an example. The uncertainty equation proposed is justified by comparing the theoretical RSD values with the experimental RSD values obtained by replication over a wide range of analyte amount. The detection limit and quantitation limit are estimated from the continuous plot (precision profile) of the theoretical RSD against analyte amount.
authors.affiliation: School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. hasegawa@ps.toyaku.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Shinohara
authors.forename: Takao
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Rieko
authors.initials: R
authors.lastname: Matsuda
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007201:Indicator Dilution Techniques; D008777:Methyltestosterone / Q000302:isolation & purification; D008962:Models, Theoretical
chemical_list: D003903:Deuterium; D008777:Methyltestosterone