Surface modification for DNA and protein microarrays.
Microarrays of biomolecules are emerging as powerful tools for genomics, proteomics, and clinical assays, since they make it possible to screen biologically important binding events in a parallel and high throughput fashion. Because the microarrays are fabricated on a solid support, coating of the surface and immobilization strategy of the biomolecules are major issues for successful microarray fabrication. This review deals with both DNA microarrays and protein microarrays, and focuses on the various modification approaches for the two-dimensional surface materials and three-dimensional ones. In addition, the immobilization strategies including adsorption, covalent attachment, physical entrapment, and affinity attachment of the biomolecules are summarized, and advantage and limitation of representative efforts are discussed.
Oh
Soon Jin
SJ
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Korea. sjoh@konkuk.ac.kr
Hong
Bong Jin
BJ
Choi
Kwan Yong
KY
Park
Joon Won
JW
D008420:Manufactured Materials; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D013499:Surface Properties