title: Qualification and performance characteristics of a quantitative enzyme-linked immunosorbent assay for human lgG antibodies to anthrax lethal factor antigen.
abstract: The contribution of Bacillus anthracis lethal factor (LF)-specific immune responses to protection against anthrax disease in humans remains incompletely defined due, in part, to a paucity of qualified reagents and a lack of standardized serological assays. Toward this end, we have identified and characterized suitable positive quality control and standard reference sera and developed, optimized, and qualified an enzyme-linked immunosorbent assay (ELISA) to measure LF-binding IgG. Herein we describe the performance characteristics of this ELISA and propose criteria for its use in the detection and quantification of anti-LF IgG in human serum.
authors.affiliation: The Biodesign Institute at Arizona State University, Tempe, AZ, USA.
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Selinsky
authors.forename: Vanessa D
authors.initials: VD
authors.lastname: Whitlow
authors.forename: Larry R
authors.initials: LR
authors.lastname: Smith
authors.forename: David C
authors.initials: DC
authors.lastname: Kaslow
authors.forename: Holly M
authors.initials: HM
authors.lastname: Horton
mesh_terms: D000881:Anthrax / Q000517:prevention & control; D000942:Antigens, Bacterial / Q000276:immunology*; D000992:Antitoxins / Q000032:analysis* / Q000627:therapeutic use; D001427:Bacterial Toxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D012015:Reference Standards; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial; D000992:Antitoxins; D001427:Bacterial Toxins; D007074:Immunoglobulin G; C030325:anthrax toxin