title: Contribution of yeast artificial chromosome-based physical maps to the final assembly of the Trypanosoma cruzi genome.
abstract: This chapter describes the methodology used both in performing the electrophoretic karyotype of the protozoan parasite Trypanosoma cruzi and mapping the genetic markers of the chromosomal bands, the construction of chromosome-specific YAC contigs, and their use to assign a chromosomal location to whole genome shotgun sequences.
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, UNIFESP, S. Paulo, Brazil.
authors.forename: Márcia R M
authors.initials: MR
authors.lastname: Santos
authors.forename: Roberto R M
authors.initials: RR
authors.lastname: Barros
authors.forename: Patrício E
authors.initials: PE
authors.lastname: Porcile
authors.forename: Mariano J
authors.initials: MJ
authors.lastname: Levin
authors.forename: José
authors.initials: J
authors.lastname: Franco da Silveira
mesh_terms: D000818:Animals; D018244:Chromosomes, Artificial, Yeast; D005819:Genetic Markers; D018503:Genome, Protozoan; D007621:Karyotyping; D020161:Physical Chromosome Mapping; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D005819:Genetic Markers