Effect of low density lipoprotein on DNA integrity of freezing-thawing boar sperm by neutral comet assay.
A modified protocol of neutral comet assay was utilized to assess the effect of low density lipoprotein (LDL) on the DNA integrity of boar freezing-thawing semen. The results demonstrated that the method was high sensitive and easier manipulation and LDL significantly protected sperm DNA integrity (p<0.05) from the damage caused by cryopreservation except TD at the concentration of 6%, 7% and TM at 6%, the optimal LDL concentration in diluents was 9%. Moreover, LDL showed better protection in 0.25 ml than in 0.5 ml types of straw (p<0.05) and no difference was observed in the same volume straw at the concentration of 9% and 10%. It was just the same for LDL effect on boar sperm DNA in cryopreservation 0 day and 30 days (p>0.05).
Jiang
Zhong-Liang
ZL
College of Animal Science & Technology, Northwest A & F University, Yangling, ShaanXi Province, PR China.
Li
Qing-Wang
QW
Li
Wen-Ye
WY
Hu
Jian-Hong
JH
Zhao
Hong-Wei
HW
Zhang
Shu-Shan
SS
D000818:Animals; D020552:Comet Assay / Q000662:veterinary*; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008297:Male; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013552:Swine / Q000502:physiology*
D003451:Cryoprotective Agents; D008077:Lipoproteins, LDL