title: Gene expression profiling of human mast cell subtypes: an in silico study.
abstract: BACKGROUND
  Human mast cells (MCs) were classified into at least two subtypes, i.e., tryptase- and chymase-positive MCs (MC(TC)) and tryptase-only-positive MCs (MC(T)). However, differences in global molecular expression between these subtypes are unknown.
  
  
  METHODS
  We analyzed public microarray data of MC subtypes derived from various tissues and those of peripheral blood granulocytes by using hierarchical clustering methods to understand the global gene expression profiles.
  
  
  RESULTS
  All the transcripts subjected to this clustering analysis were classified into two large clusters, i.e., MC-preferential or granulocyte-preferential. In the original works, MCs from tonsil, lung and skin had been cultured for more than several weeks to obtain highly viable and pure cell populations, and these MCs retained their typical profiles such as intensities of chymase protein expression. Most of the transcripts were commonly expressed by these MC subtypes. However, tonsil-derived MCs and skin-derived MCs but not lung-derived MCs expressed high levels of chymase (CMA1) as expected for the properties of MC(TC) and MC(T). These CMA1-high MCs and CMA1-low MCs respectively expressed distinct sets of transcripts as small gene clusters as well as CMA-1 even after being cultured in the absence of a tissue environment.
  
  
  CONCLUSIONS
  The MC lineage seems to be far from the granulocyte lineages including basophils. CMA1-high MCs (MC(TC)) and CMA1-low MCs (MC(T)) can be regarded as differentiated MC subtypes. As such, importance of data analysis studies will be increasing along with the accumulation of global molecular data in the public database.
authors.affiliation: Department of Allergy & Immunology, National Research Institute for Child Health & Development, Tokyo, Japan. hsaito@nch.go.jp
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Saito
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Shigeru
authors.initials: S
authors.lastname: Okumura
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Kashiwakura
authors.forename: Keisuke
authors.initials: K
authors.lastname: Oboki
authors.forename: Hidenori
authors.initials: H
authors.lastname: Yokoi
authors.forename: Naotomo
authors.initials: N
authors.lastname: Kambe
authors.forename: Ken
authors.initials: K
authors.lastname: Ohta
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Okayama
mesh_terms: D002478:Cells, Cultured; D053818:Chymases / Q000096:biosynthesis / Q000235:genetics; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D008407:Mast Cells / Q000737:chemistry* / Q000145:classification* / Q000201:enzymology; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D053818:Chymases