Cloning of DOG1, a quantitative trait locus controlling seed dormancy in Arabidopsis.
Genetic variation for seed dormancy in nature is a typical quantitative trait controlled by multiple loci on which environmental factors have a strong effect. Finding the genes underlying dormancy quantitative trait loci is a major scientific challenge, which also has relevance for agriculture and ecology. In this study we describe the identification of the DELAY OF GERMINATION 1 (DOG1) gene previously identified as a quantitative trait locus involved in the control of seed dormancy. This gene was isolated by a combination of positional cloning and mutant analysis and is absolutely required for the induction of seed dormancy. DOG1 is a member of a small gene family of unknown molecular function, with five members in Arabidopsis. The functional natural allelic variation present in Arabidopsis is caused by polymorphisms in the cis-regulatory region of the DOG1 gene and results in considerable expression differences between the DOG1 alleles of the accessions analyzed.
Bentsink
Leónie
L
Laboratory of Genetics, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands.
Jowett
Jemma
J
Hanhart
Corrie J
CJ
Koornneef
Maarten
M
D000483:Alleles; D017398:Alternative Splicing; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D014644:Genetic Variation; D018525:Germination / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D040641:Quantitative Trait Loci; D012639:Seeds / Q000254:growth & development / Q000502:physiology; D014158:Transcription, Genetic
D018744:DNA, Plant