Core promoter elements recognized by transcription factor IIB.
The general transcription factor TFIIB (transcription factor IIB) plays a critical role in the assembly of the RNA polymerase II pre-initiation complex. TFIIB can make sequence-specific DNA contacts both upstream and downstream of the TATA box. This has led to the definition of two core promoter BREs (TFIIB-recognition elements), one upstream [BRE(u) (upstream BRE)] and one downstream of TATA box [BRE(d) (downstream BRE)]. TFIIB-BRE(u) and TFIIB-BRE(d) contacts are mediated by two independent DNA-recognition motifs within the core domain of TFIIB. Both the BRE(u) and the BRE(d) modulate the transcriptional potency of a promoter. However, the net effect of the BREs on promoter activity is dependent on the specific blend of elements present within a core promoter.
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Faculty of Life Sciences, The Michael Smith Building, The University of Manchester, Oxford Road, Manchester M13 9PT, UK.
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D001665:Binding Sites; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics*; D016385:TATA Box; D035581:Transcription Factor TFIIB / Q000235:genetics / Q000378:metabolism*
D035581:Transcription Factor TFIIB; D012319:RNA Polymerase II