title: Plasmodium yoelii yoelii 17XNL constitutively expressing GFP throughout the life cycle.
abstract: Plasmodium yoelii is a rodent parasite commonly used as a model to study malaria infection. It is the preferred model parasite for liver-stage immunological studies and is also widely used to study hepatocyte, erythrocyte and mosquito infection. We have generated a P. yoelii yoelii 17XNL line that is stably transfected with the green fluorescent protein (gfp) gene. This parasite line constitutively expresses high levels of GFP during the complete parasite life cycle including liver, blood and mosquito stages. These fluorescent parasites can be used in combination with fluorescence activated cell sorting or live microscopy for a wide range of experimental applications.
authors.affiliation: New York University School of Medicine, Department of Medical Parasitology, New York, NY 10010, USA.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ono
authors.forename: Takushi
authors.initials: T
authors.lastname: Tadakuma
authors.forename: Ana
authors.initials: A
authors.lastname: Rodriguez
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology; D000962:Antimalarials / Q000494:pharmacology; D004195:Disease Models, Animal; D004351:Drug Resistance; D004912:Erythrocytes / Q000469:parasitology; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D007303:Insect Vectors / Q000469:parasitology; D008018:Life Cycle Stages / Q000235:genetics / Q000502:physiology*; D008099:Liver / Q000469:parasitology; D008288:Malaria / Q000469:parasitology*; D051379:Mice; D010967:Plasmodium yoelii / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D011739:Pyrimethamine / Q000494:pharmacology; D014162:Transfection
chemical_list: D000962:Antimalarials; D049452:Green Fluorescent Proteins; D011739:Pyrimethamine