Potide-G derived from potato (Solanum tuberosum L.) is active against potato virus YO (PVYO) infection.
A PVYO virus-resistant potato (Solanum tuberosum L. cv. Golden Valley) was identified, and further, from its tubers, a small (5.57 kDa) antiviral peptide potide-G was isolated. Application of potide-G on virus susceptible potato (cv. Winter valley) expressed robust resistance to PVYO infection and showed no virus infected morphology. We found that PVYO infection spreads up completely within 3 days post inoculation (dpi) in susceptible cultivar. PVYO was more accumulated toward the basal leaves, when infection occurred longer. Combined results of morphology of PVYO infection, ELISA, RT-PCR, and real-time PCR showed the resistance to the PVYO infection depends on the expression of Ry gene. Indeed, the real-time PCR result showed that the Ry gene up-regulated to 3 times higher in PVYO infected cv. Golden valley. Golden crude protein was found to be active against PVYO infection in the in vivo test. In addition, application of potide-G in a virus susceptible potato potently reduced the viral infection actively with 50 times lower concentration than that of the Golden protein. Further identification of a host-specific resistant gene in a plant and the peptide derived from it offers new opportunities for the development of novel bio-pesticides against plant virus.
Tripathi
Giri Raj
GR
Department of Biotechnology and BK21 Research Team for Protein Activity Control, and Research Center for Proteineous Materials (RCPM), Chosun University, Gwangju 501-759, Republic of Korea.
Park
Jaekyun
J
Park
Youngmin
Y
Hwang
Indeok
I
Park
Yoonkyung
Y
Hahm
Kyung-Soo
KS
Cheong
Hyeonsook
H
D036022:Capsid Proteins / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D018506:Gene Expression Regulation, Plant; D015967:Gene Expression Regulation, Viral; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology*; D010935:Plant Diseases / Q000821:virology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000378:metabolism / Q000821:virology; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D010942:Plant Viruses / Q000145:classification / Q000187:drug effects* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011198:Solanum tuberosum / Q000737:chemistry* / Q000378:metabolism / Q000821:virology*
D036022:Capsid Proteins; D010455:Peptides; D010936:Plant Extracts; D010940:Plant Proteins; D012333:RNA, Messenger