title: Autologous transplantation of mononuclear bone marrow cells in patients with acute myocardial infarction: the effect of the dose of transplanted cells on myocardial function.
abstract: BACKGROUND
  Despite the reports on successful treatment of acute myocardial infarction using autologous mononuclear bone marrow cell transplantation, many unresolved questions still remain. We studied the impact of the dose of transplanted cells on myocardial function and perfusion.
  
  
  METHODS
  Sixty-six patients with a first acute myocardial infarction were randomized into 3 groups. Two groups were intracoronarily given mononuclear bone marrow cells in either higher (10(8) cells, higher cell dose [HD] group, n = 22) or lower (10(7) cells, lower cell dose [LD] group, n = 22) doses. Twenty-two patients without cell transplantation served as a control (C) group.
  
  
  RESULTS
  At 3 months of follow-up, the baseline peak systolic velocities of longitudinal contraction of the infarcted wall of 5.2, 4.5, and 4.3 cm/s in C, LD, and HD groups increased by 0.0, 0.5 (P < .05 vs C group), and 0.9 cm/s (P < .05 vs LD group, P < .01 vs C group), respectively, as demonstrated by Doppler tissue imaging. Baseline left ventricular ejection fractions of 42%, 42%, and 41% in C, LD, and HD groups increased by 2%, 3%, and by 5% (P < .05 vs group C), respectively, as assessed by the gated technetium Tc 99m sestamibi single photon emission computed tomography.
  
  
  CONCLUSIONS
  Mononuclear bone marrow cell transplantation improves regional myocardial function of the infarcted wall in a dose-dependent manner.
authors.affiliation: 1st Department of Internal Medicine/Cardioangiology, St. Anna Hosp., Brno, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Meluzín
authors.forename: Jirí
authors.initials: J
authors.lastname: Mayer
authors.forename: Ladislav
authors.initials: L
authors.lastname: Groch
authors.forename: Stanislav
authors.initials: S
authors.lastname: Janousek
authors.forename: Ivan
authors.initials: I
authors.lastname: Hornácek
authors.forename: Ota
authors.initials: O
authors.lastname: Hlinomaz
authors.forename: Petr
authors.initials: P
authors.lastname: Kala
authors.forename: Roman
authors.initials: R
authors.lastname: Panovský
authors.forename: Jirí
authors.initials: J
authors.lastname: Prásek
authors.forename: Milan
authors.initials: M
authors.lastname: Kamínek
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Stanícek
authors.forename: Martin
authors.initials: M
authors.lastname: Klabusay
authors.forename: Zdenik
authors.initials: Z
authors.lastname: Korístek
authors.forename: Milan
authors.initials: M
authors.lastname: Navrátil
authors.forename: Ladislav
authors.initials: L
authors.lastname: Dusek
authors.forename: Jaroslava
authors.initials: J
authors.lastname: Vinklárková
mesh_terms: D016026:Bone Marrow Transplantation; D005260:Female; D006321:Heart / Q000503:physiopathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009200:Myocardial Contraction; D009203:Myocardial Infarction / Q000601:surgery*; D009206:Myocardium; D014182:Transplantation, Autologous