Specific short hairpin RNA-mediated inhibition of viral DNA packaging of human cytomegalovirus.
To clearly demonstrate the role of the human cytomegalovirus (HCMV) portal protein pUL104 RNA interference was applied. Expressing cell lines were constructed by transduction of shRNAs via the infection with retroviral vectors. After infection of these cells with HCMV AD169 the expression of pUL104 was reduced up to 80% for shRNA S1 and 54% for shRNA S2 at late times of infection compared to controls. In addition, the inhibitory effect was corresponding with a decrease in viral mRNA and plaque formations. Electron microscopic analysis demonstrated that infection of cells expressing pUL104-specific shRNAs resulted in the inhibition of formation of replicative particles.
Dittmer
Alexandra
A
Institute of Virology, Charité Universitätsmedizin Berlin, Charité Campus Mitte, Charitéplatz 1, 10117 Berlin, Germany.
Bogner
Elke
E
D002460:Cell Line; D003587:Cytomegalovirus / Q000187:drug effects / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000188:drug therapy; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology*; D012367:RNA, Viral / Q000187:drug effects*; D010948:Viral Plaque Assay; D014771:Virion / Q000187:drug effects; D019065:Virus Assembly / Q000187:drug effects*
D034741:RNA, Small Interfering; D012367:RNA, Viral