Differential expression of anti-Müllerian hormone (amh) and anti-Müllerian hormone receptor type II (amhrII) in the teleost medaka.
In mammals, the anti-Müllerian hormone (Amh) is responsible for the regression of the Müllerian ducts; therefore, Amh is an important factor of male sex differentiation. The amh gene has been cloned in various vertebrates, as well as in several teleost species. To date, all described species show a sexually dimorphic expression of amh during sex differentiation or at least in differentiated juvenile gonads. We have identified the medaka amh ortholog and examined its expression pattern. Medaka amh shows no sexually dimorphic expression pattern. It is expressed in both developing XY male and XX female gonads. In adult testes, amh is expressed in the Sertoli cells and in adult ovaries in granulosa cells surrounding the oocytes, like in mammals. To better understand the function of amh, we cloned the anti-Müllerian hormone receptor type II (amhrII) ortholog and compared its expression pattern with amh, aromatase (cyp19a1), and scp3. During gonad development, amhrII is coexpressed with medaka amh in somatic cells of the gonads and shows no sexually dimorphic expression. Only the expression level of the Amh type II receptor gene was decreased noticeably in adult female gonads. These results suggest that medaka Amh and AmhrII are involved in gonad formation and maintenance in both sexes.
Klüver
Nils
N
University of Würzburg, Department of Physiological Chemistry I, Biozentrum, Würzburg, Germany.
Pfennig
Frank
F
Pala
Irene
I
Storch
Katja
K
Schlieder
Marlen
M
Froschauer
Alexander
A
Gutzeit
Herwig O
HO
Schartl
Manfred
M
D000595:Amino Acid Sequence; D000818:Animals; D054304:Anti-Mullerian Hormone; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009990:Oryzias / Q000145:classification / Q000196:embryology* / Q000378:metabolism; D010053:Ovary / Q000196:embryology / Q000378:metabolism; D010802:Phylogeny; D018000:Receptors, Peptide / Q000235:genetics* / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta; D016415:Sequence Alignment; D013735:Testicular Hormones / Q000235:genetics* / Q000378:metabolism; D013737:Testis / Q000196:embryology / Q000378:metabolism
D029941:Fish Proteins; D006023:Glycoproteins; D018000:Receptors, Peptide; D018125:Receptors, Transforming Growth Factor beta; D013735:Testicular Hormones; C084621:anti-Mullerian hormone receptor; D054304:Anti-Mullerian Hormone; D001141:Aromatase