title: LexA represses CTXphi transcription by blocking access of the alpha C-terminal domain of RNA polymerase to promoter DNA.
abstract: CTXPhi is a Vibrio cholerae-specific temperate filamentous phage that encodes cholera toxin. CTXPhi lysogens can be induced with DNA damage-inducing agents such as UV light, leading to the release of CTXPhi virions and the rapid dissemination of cholera toxin genes to new V. cholerae hosts. This environmental regulation is directly mediated by LexA, the host-encoded global SOS transcription factor. LexA and a phage-encoded repressor, RstR, both repress transcription from P(rstA), the primary CTXPhi promoter. Because the LexA binding site is located upstream of the core P(rstA) promoter and overlaps with A-tract sequences, we speculated that LexA represses P(rstA) by occluding a promoter UP element, a binding site for the C-terminal domain of the alpha subunit of RNA polymerase (RNAP) (alphaCTD). Using in vitro transcription assays, we have shown that the LexA binding site stimulates maximal rstA transcription in the absence of any added factors. The alphaCTD of RNAP is required for this stimulation, demonstrating that the LexA site contains, or overlaps with, a promoter UP element. LexA represses rstA transcription by normal RNAP but fails to repress rstA transcription catalyzed by RNAP lacking the alphaCTD. DNase I footprint analysis mapped the alphaCTD binding site to the upstream promoter region that includes the LexA binding site. The addition of free alpha subunits blocked the binding of LexA to rstA promoter DNA, indicating that LexA and the alphaCTD directly compete for binding to their respective sites. To our knowledge, this is the first report of a repressor blocking transcription initiation by occluding a promoter UP element.
authors.affiliation: Department of Molecular Microbiology, Tufts University School of Medicine and the Howard Hughes Medical Institute, Boston, Massachusetts 02111, USA.
authors.forename: Mariam
authors.initials: M
authors.lastname: Quinones
authors.forename: Harvey H
authors.initials: HH
authors.lastname: Kimsey
authors.forename: Wilma
authors.initials: W
authors.lastname: Ross
authors.forename: Richard L
authors.initials: RL
authors.lastname: Gourse
authors.forename: Matthew K
authors.initials: MK
authors.lastname: Waldor
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D001665:Binding Sites; D002384:Catalysis; D002772:Cholera Toxin / Q000737:chemistry* / Q000378:metabolism*; D004247:DNA / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000737:chemistry; D003851:Deoxyribonucleases / Q000737:chemistry; D017912:Inovirus / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000378:metabolism; D012697:Serine Endopeptidases / Q000737:chemistry / Q000502:physiology*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D002772:Cholera Toxin; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D003850:Deoxyribonuclease I; D003851:Deoxyribonucleases; D012097:Repressor Proteins; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein; C107871:RstR protein, Vibrio cholerae