title: Nasal augmentation using injectable alginate and mesenchymal stem cells in the rabbit.
abstract: BACKGROUND
  The goal of this study was to evaluate the use of the autogenous mesenchymal stem cells (MSCs) impregnated in an injectable alginate gel containing platelet-rich plasma (PRP) for nasal augmentation in rabbit model.
  
  
  METHODS
  Bone marrow-derived MSCs were isolated and expanded from New Zealand white rabbits. At confluence, the cells were mixed with sodium alginate solution. PRP was prepared from the rabbits and it was immediately mixed into the alginate-cell mixture. The cell-PRP-alginate mix was injected into a subcutaneous nasal area. Eight weeks after injection changes in facial contour, newly formed nasal hump was analyzed and the amount of chondroitin sulfate in tissue was measured.
  
  
  RESULTS
  Augmented nasal dorsa maintained their original shape until harvest. Immunohistochemical staining revealed that the deposited matrix was composed of type II collagen and that it was distributed abundantly and widely in the connective tissue of the tissue generated. The amount of chondroitin sulfate (main component of the proteoglycan in cartilage) produced was significantly higher when MSCs and PRP-alginate were used.
  
  
  CONCLUSIONS
  Injectable PRP-alginate gel containing autologous mesenchymal stem cells may offer a useful means of facial soft tissue augmentation.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Yonsei University Wonju College of Medicine, Korea. rhico@yonsei.ac.kr
authors.forename: Dong-Joon
authors.initials: DJ
authors.lastname: Park
authors.forename: Jin Hyun
authors.initials: JH
authors.lastname: Choi
authors.forename: Yoo-Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jin-Hyung
authors.initials: JH
authors.lastname: Kim
authors.forename: Ki-Taek
authors.initials: KT
authors.lastname: Kim
mesh_terms: D000464:Alginates / Q000008:administration & dosage*; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage*; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000032:analysis / Q000096:biosynthesis; D024043:Collagen Type II / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005782:Gels; D007150:Immunohistochemistry; D007279:Injections, Subcutaneous; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009666:Nose / Q000601:surgery*; D053657:Platelet-Rich Plasma / Q000166:cytology; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D014182:Transplantation, Autologous; D016896:Treatment Outcome
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D024043:Collagen Type II; D005782:Gels; D002809:Chondroitin Sulfates