title: [Study on separation and purification of anthraquinones in radix et rhizoma rhei by D301 macroporous resin].
abstract: OBJECTIVE
  The parameters of absorption and purification of total anthraquinones in Radix Et Rhizoma Rhei by D301 macroporous resin were investigated in this paper.
  
  
  METHODS
  HPLC was used to analyze the content of total anthraquinones in Radix Et Rhizoma with emodin as control.
  
  
  RESULTS
  The appropriate adsorption conditions were: concentration of extract 0.5 g x mL(-1); pH 9; flow rate 1 BV x h(-1). When the 75% ethanol was used as elution and the concentration of HCl was 0.1 mol x L(-1), and the flow rate was 1.0 BV x h(-1), the effect of desorption was satisfactory.
  
  
  CONCLUSIONS
  D301 resin provided a good effect on the exchang and absorption of total anthraquinones in Radix Et Rhizoma.
authors.affiliation: Hubei College of Traditional Chinese Medicine, Wuhan 430061, China. xhl4201@sina.com
authors.forename: Han-Lin
authors.initials: HL
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Ji-Zhen
authors.initials: JZ
authors.lastname: Shao
authors.forename: Yun
authors.initials: Y
authors.lastname: Sun
authors.forename: Chi-Zhi
authors.initials: CZ
authors.lastname: Zhang
mesh_terms: D000880:Anthraquinones / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic; D012250:Rheum / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry
chemical_list: D000880:Anthraquinones; D012117:Resins, Synthetic