Genome wide cDNA-AFLP analysis of genes rapidly induced by combined sucrose and ABA treatment in rice cultured cells.
We identified 27 genes induced by combined sucrose and ABA treatment from rice cultured cells with cDNA-AFLP. Thirteen of these up-regulated genes were induced 30 min after the co-treatment. This suite of genes includes starch biosynthesis related genes. Type A genes were expressed only in the presence of both sucrose and ABA. Type B genes were expressed in the presence of sucrose or ABA and the expression was dramatically enhanced by the co-treatment of sucrose and ABA. These results indicate that multiple steps of starch biosynthesis and other processes may be regulated by at least two different pathways.
Akihiro
Takashi
T
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan. takihiro@mua.biglobe.ne.jp
Umezawa
Taishi
T
Ueki
Chie
C
Lobna
Ben Mohamed
BM
Mizuno
Kouichi
K
Ohta
Masaru
M
Fujimura
Tatsuhito
T
D000040:Abscisic Acid / Q000494:pharmacology*; D001483:Base Sequence; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000187:drug effects*; D018745:Genome, Plant; D021141:Nucleic Acid Amplification Techniques; D012275:Oryza / Q000187:drug effects* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013395:Sucrose / Q000494:pharmacology*
D018076:DNA, Complementary; D018744:DNA, Plant; D013395:Sucrose; D000040:Abscisic Acid