title: Effects of cardiac patches engineered with bone marrow-derived mononuclear cells and PGCL scaffolds in a rat myocardial infarction model.
abstract: Little is known about the cardioprotective effects against heart failure (HF), the effects on differentiation of bone marrow-derived mononuclear cell (BMMNC), and the biocompatibility of BMMNC-seeded biodegradable poly-glycolide-co-caprolactone (PGCL) scaffolds in a myocardial infarction (MI) animal model. This study hypothesized that implantation of a BMMNC-seeded PGCL scaffold into the epicardial surface in a rat MI model would be biocompatible, induce BMMNC migration into infarcted myocardium, and effectively improve left ventricular (LV) systolic dysfunction. One week after the implantation of a BMMNC-seeded PGCL scaffold, BMMMC showed migration into the epicardial region. Four weeks after implantation, augmented neovascularization was observed in infarcted areas and in infarct border zones. Some BMMNCs exhibited the presence of alpha-MHC and troponin I, markers of differentiation into cardiomyocytes. In echocardiographic examinations, BMMNC-seeded PGCL scaffold and non-cell-seeded simple PGCL scaffold groups effectively reduced progressive LV dilatation and preserved LV systolic function as compared to control rat MI groups. Thus, BMMNC-seeded PGCL scaffolding influences BMMNC migration, differentiation to cardiomyocytes, and induction of neovascularization, ultimately effectively lessening LV remodeling and progressive LV systolic dysfunction. PGCL scaffolding can be considered as an effective treatment alternative in MI-induced advanced HF.
authors.affiliation: Department of Cardiology, College of Medicine, Chungbuk National University, 62 Gaesin-dong, Heungdeok-gu, Cheongju 361-763, Korea.
authors.forename: Hainan
authors.initials: H
authors.lastname: Piao
authors.forename: Jin-Sook
authors.initials: JS
authors.lastname: Kwon
authors.forename: Shuguang
authors.initials: S
authors.lastname: Piao
authors.forename: Ju-Hee
authors.initials: JH
authors.lastname: Sohn
authors.forename: Yeong-Shin
authors.initials: YS
authors.lastname: Lee
authors.forename: Jang-Whan
authors.initials: JW
authors.lastname: Bae
authors.forename: Kyung-Kuk
authors.initials: KK
authors.lastname: Hwang
authors.forename: Dong-Woon
authors.initials: DW
authors.lastname: Kim
authors.forename: Oju
authors.initials: O
authors.lastname: Jeon
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Young-Bae
authors.initials: YB
authors.lastname: Park
authors.forename: Myeong-Chan
authors.initials: MC
authors.lastname: Cho
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004562:Electrocardiography; D008855:Microscopy, Electron, Scanning; D009000:Monocytes / Q000166:cytology* / Q000637:transplantation*; D009203:Myocardial Infarction / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D011091:Polyesters; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D011091:Polyesters; C516442:poly(glycolide-co-caprolactone)