title: Reverse remodeling of cardiac collagen protein expression after surgical therapy for experimental aortic stenosis.
abstract: OBJECTIVE
  Reversal of myocardial collagen gene expression was examined in parallel to left ventricular reverse remodeling after surgical correction of experimental aortic stenosis.
  
  
  METHODS
  A standard growing sheep model (age at baseline 6-8 months) was used. Measurements were performed at baseline (point A) when inducing left ventricular hypertrophy (LVH) through supracoronary banding, at 8.3 +/- 1.0 months later at surgical correction (point B), and after another 10.1 +/- 2.0 months during final examinations (point C). Gene expression for collagen I and III was also studied.
  
  
  RESULTS
  Left ventricular function was stable throughout the study. The left ventricular mass index (LVMI) was 82 +/- 21 g/m2 at point A, 150 +/- 33 g/m2 at B, and 78 +/- 18 g/m2 at C (p <0.01). Myocardial fiber diameter was 11.3 +/- 0.8, 15.9 +/- 1.2 and 11.4 +/- 1 microm at points A, B and C, respectively (p <0.01). Protein expression for collagen I was 0.71 +/- 0.2 (at A), 1.13 +/- 0.3 (at B) and 0.85 +/- 0.4 (at C) (p <0.01), while that for collagen III was 0.72 +/- 0.4 (at A), 1.26 +/- 0.8 (at B) and 0.83 +/- 0.3 (at C) (p <0.01). There was a significant correlation between changes in LVMI and myocardial collagen expression.
  
  
  CONCLUSIONS
  Complete reverse remodeling with regression of LVH and myocardial collagen protein expression can be anticipated after surgical correction of experimental aortic stenosis.
authors.affiliation: University of Leipzig, Heart Center, Clinic for Heart Surgery, Leipzig, Germany. walt@medizin.uni-leipzig.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Walther
authors.forename: Andreas
authors.initials: A
authors.lastname: Schubert
authors.forename: Thomas
authors.initials: T
authors.lastname: Wustmann
authors.forename: Volkmar
authors.initials: V
authors.lastname: Falk
authors.forename: Claudia
authors.initials: C
authors.lastname: Walther
authors.forename: Nicolas
authors.initials: N
authors.lastname: Doll
authors.forename: Ardawan
authors.initials: A
authors.lastname: Rastan
authors.forename: Jan
authors.initials: J
authors.lastname: Gummert
authors.forename: Friedrich W
authors.initials: FW
authors.lastname: Mohr
mesh_terms: D000818:Animals; D001024:Aortic Valve Stenosis / Q000150:complications / Q000378:metabolism* / Q000473:pathology / Q000601:surgery*; D015153:Blotting, Western; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D019918:Heart Valve Prosthesis Implantation; D017379:Hypertrophy, Left Ventricular / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000378:metabolism*; D012756:Sheep; D016277:Ventricular Function, Left; D020257:Ventricular Remodeling
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III