Guanidinylated allylamine-N-isopropylacrylamide copolymer nonviral transgene vectors.
N-Isopropylacrylamide and allylamine copolymers (PNIALM) were prepared by radical polymerization method. To endow them with arginine-like cell membrane penetrating function, the aminos in PNIALMs were transformed to guanidinium groups by chemical modification. The formation and guanidinylation PNIALM were confirmed by NMR; the composition of copolymers and the degree of substitution of guanidino in modified copolymer (PNIALM-G) were estimated as well. The electrophoretic assay revealed that PNIALM-G was capable of condensing DNA in spite of lower binding affinity compared to its parent copolymers. The results of particle size analyzer and TEM indicated that at higher copolymer/DNA weight ratios, the copolymer/DNA complexes were condensed to nanoparticles. PNIALM-G1-3 was shown to be very efficient in mediating plasmid DNA transfection to COS-1 cells both in the presence and absence of serum, even superior to PEI.
Zhang
Bingqi
B
School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China.
Ji
Weihang
W
Liu
Wenguang
W
Yao
Kangde
K
D000178:Acrylamides / Q000737:chemistry*; D000499:Allylamine / Q000737:chemistry*; D000818:Animals; D019556:COS Cells; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000737:chemistry* / Q000633:toxicity; D019791:Guanidine / Q000737:chemistry*; D008156:Luciferases / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D011108:Polymers / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection / Q000379:methods*; D019076:Transgenes
D000178:Acrylamides; D011108:Polymers; D000499:Allylamine; D004247:DNA; C067295:N-isopropylacrylamide; D008156:Luciferases; D019791:Guanidine