Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection.
Colombian strain of Trypanosoma cruzi, biodeme Type III (T. cruzi I), has been cloned by micromanipulation at two phases of the acute infection: early (10 days ) and advanced (30 days). Twelve clones were obtained therefrom. Characterization by their biological and biochemical behavior showed an identity among the several clones and their parental strain, albeit with different degrees of virulence. Molecular characterization of the kinetoplast DNA (kDNA) after amplification by polymerase chain reaction revealed identical profiles of the bands from the kDNA minicircle by the analysis of restriction fragment length polymorphism for the isolated clones, their parental strain, and to the clones isolated at two different phases of the infection. Results suggest the predominance of a "principal clone", in the composition of the Colombian strain, responsible for the biological and biochemical behavior. However, no relationship was detected between the molecular profile of kDNA and the degree of virulence presented by the several clones.
Camandaroba
Edson L P
EL
Laboratorio de Histologia e Embriologia Animal Comparada, Universidade Estadual de Feira de Santana, Feira de Santana, BA, Brazil.
Reis
Eliana A G
EA
Reis
Mitermayer G
MG
Andrade
Sonia G
SG
D000208:Acute Disease; D000818:Animals; D014355:Chagas Disease / Q000469:parasitology; D002999:Clone Cells; D003105:Colombia; D018105:DNA, Kinetoplast / Q000235:genetics*; D051379:Mice; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
D018105:DNA, Kinetoplast