In vitro evaluation of the mucoadhesive properties of polysaccharide-based nanoparticulate oral drug delivery systems.
Impedance quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) measurements were performed in order to assess the mucoadhesive properties of hydrophobically modified (HM) derivatives of dextran (DEX), with an average molecular weight of 10,000 Da, and of hydroxypropylcellulose (HPC), with an average molecular weight of 80,000 Da. The measurements involved (1) treatment of a hydrophobic surface with bovine submaxillary gland mucin (BSM) under various pH conditions (2.0-8.0) and (2) treatment of the BSM layer with buffer solutions of the amphiphilic polysaccharides (pH 3.0 and 7.0). Control measurements were carried out with DEX, HPC, and chitosan (CH) used as a model mucoadhesive polymer. All HM-polysaccharides were shown to adsorb onto a BSM layer, the extent of adsorption increasing with increasing hydrophobicity of the samples. Under the same conditions, HPC and CH interacted with the BSM layer, but DEX showed no affinity to BSM. All the results suggest that HM-polysaccharide micellar systems have the potential of enhancing the bioavailability of poorly adsorbed drugs in peroral delivery.
Chayed
Siwar
S
Faculty of Pharmacy and Department of Chemistry, University of Montreal, Montreal, QC, Canada.
Winnik
Françoise M
FM
D000268:Adhesiveness; D000284:Administration, Oral; D000327:Adsorption; D000818:Animals; D002021:Buffers; D002417:Cattle; D048271:Chitosan / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry* / Q000378:metabolism; D004337:Drug Carriers; D004563:Electrochemistry / Q000295:instrumentation; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D065347:Hypromellose Derivatives; D007700:Kinetics; D008747:Methylcellulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D008823:Micelles; D008970:Molecular Weight; D009077:Mucins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D053758:Nanoparticles; D004364:Pharmaceutical Preparations / Q000008:administration & dosage / Q000737:chemistry; D011485:Protein Binding; D011791:Quartz; D013363:Submandibular Gland / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013696:Temperature
D002021:Buffers; D003911:Dextrans; D004337:Drug Carriers; D008823:Micelles; D009077:Mucins; D004364:Pharmaceutical Preparations; D013438:Sulfhydryl Compounds; C013976:dodecylmercaptan; D011791:Quartz; D065347:Hypromellose Derivatives; D008747:Methylcellulose; D048271:Chitosan