A proteomic analysis of Streptomyces coelicolor programmed cell death.
Programmed cell death (PCD) is an active cellular suicide that occurs in eukaryotes and bacteria in response to both abiotic and biotic stresses. In contrast to eukaryotic apoptosis, little is known about the molecular machinery that regulates bacterial PCD. In a previous work, we described the existence of PCD phenomena in Streptomyces (Manteca et al., Res. Microbiol. 2006, 157, 143-152). In the present study, we performed a proteomic analysis of PCD in Streptomyces coelicolor, for which we developed a system to obtain dead and live cell-enriched samples. PCD in this filamentous bacterium is accompanied by the appearance of enzymes involved in the degradation of cellular macromolecules, regulatory proteins, and stress-induced proteins. We argue that some of these proteins have specific functions in the PCD pathway and putative roles for the identified proteins have been proposed. The increased amounts of several antioxidant proteins suggest oxidative stress as either the cause or consequence of the cell death.
Manteca
Angel
A
Area de Microbiologia, Departamento de Biologia Funcional and IUBA, Facultad de Medicina, Universidad de Oviedo, Oviedo, Spain.
Mäder
Ulrike
U
Connolly
Bernard A
BA
Sanchez
Jesus
J
D017209:Apoptosis; D001426:Bacterial Proteins / Q000145:classification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D053938:DNA Fragmentation; D003851:Deoxyribonucleases / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000145:classification / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012270:Ribosomes / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology
D001426:Bacterial Proteins; D020543:Proteome; D003851:Deoxyribonucleases