title: [Determination of scopolamine and atropine in Flos Daturae by RP-HPLC].
abstract: OBJECTIVE
  To develop a quantitative analytical procedure of scopolamine and atropine in Flos Daturae using RP-HPLC.
  
  
  METHODS
  The two alkaloids were separated on a Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm) with a mobile phase of 0.02 mol x L(-1) sodium acetate buffer (containing 0.02% triethanolamine and the pH was adjusted to 6.0 with acetic acid)-methanol (60:40) and a detection wavelength of 215 nm. The flow rate was 1.0 mL x min(-1) and the column temperature was maintained at room temperature.
  
  
  RESULTS
  The mean recovery was (99.6 +/- 1.8)% for scopolamine and (100.4 +/- 1.5)% for atropine.
  
  
  CONCLUSIONS
  This method was simple, accurate and sensitive.
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing, China.
authors.forename: Shi-guo
authors.initials: SG
authors.lastname: Hou
authors.forename: Xue-xin
authors.initials: XX
authors.lastname: Gu
authors.forename: Shu-yan
authors.initials: SY
authors.lastname: Wang
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Li
mesh_terms: D001285:Atropine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032302:Datura / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012601:Scopolamine / Q000032:analysis*
chemical_list: D001285:Atropine; D012601:Scopolamine