Introduction of single mutation changes arylmalonate decarboxylase to racemase.
The introduction of only one mutation based on the estimated reaction mechanism endowed arylmalonate decarboxylase with a racemase activity, which catalyses racemisation of alpha-arylpropionates.
Terao
Yosuke
Y
Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Yokohama, 223-8522, Japan.
Miyamoto
Kenji
K
Ohta
Hiromichi
H
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D002262:Carboxy-Lyases / Q000737:chemistry*; D002384:Catalysis; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D011422:Propionates / Q000138:chemical synthesis* / Q000737:chemistry; D019998:Racemases and Epimerases / Q000737:chemistry*; D013237:Stereoisomerism; D013379:Substrate Specificity
D011422:Propionates; D002262:Carboxy-Lyases; C071852:malonate decarboxylase; D019998:Racemases and Epimerases