title: Microemulsion electrokinetic chromatography separation by using hexane-in-water microemulsions without cosurfactant: comparison with MEKC.
abstract: A new hexane-in-water microemulsion was investigated as buffer in microemulsion EKC (MEEKC). At difference with other microemulsions, the addition of cosurfactant was not necessary to stabilize the microemulsion. The proposed microemulsion was successfully used to achieve electrophoretic separation of seven antibiotics including nitroimidazoles, cephapirin and tetracyclines. Selectivity and separation efficiency achieved in MEEKC were compared with MEKC. MEEKC technique proved to be more efficient than MEKC for performing the separation of the analytes and the presence of microemulsions was found to be critical to achieve the separation of tetracyclines. The proposed microemulsion also points out that solvents with high volatility, such as hexane, can be stabilized and used as a microemulsion of SDS.
authors.affiliation: Department of Analytical Chemistry, University of Córdoba, Campus de Rabanales, Córdoba, Spain.
authors.forename: Leonor
authors.initials: L
authors.lastname: Nozal
authors.forename: Lourdes
authors.initials: L
authors.lastname: Arce
authors.forename: Bartolomé M
authors.initials: BM
authors.lastname: Simonet
authors.forename: Angel
authors.initials: A
authors.lastname: Ríos
authors.forename: Miguel
authors.initials: M
authors.lastname: Valcárcel
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D002021:Buffers; D002845:Chromatography / Q000379:methods*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004655:Emulsions / Q000737:chemistry; D006586:Hexanes / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D002021:Buffers; D004655:Emulsions; D006586:Hexanes; D012997:Solvents; D014867:Water; C026385:n-hexane