title: [The serodiagnostic value of antigens secreted from Mycobacterium tuberculosis].
abstract: OBJECTIVE
  To study the serodiagnostic value of 3 antigens secreted from Mycobacterium tuberculosis: the 81,000 antigen, the 38,000 antigen and the CF-10 antigen.
  
  
  METHODS
  The 38,000 antigen was purified from supernatant of exponential growth of Mycobacterium tuberculosis. The 81,000 antigen and CF-10 antigen were expressed by fusion with 6 histidine and purified by nickel chelate chromatographic column. Sera were obtained from 149 patients with tuberculosis (108 smear positive and 41 smear negative), 24 patients with non-tuberculosis lung diseases and 170 healthy human controls in Anhui province from March 2002 to April 2005. Antigenicity and specificity of the 3 antigens were detected by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Purified antigens had a high purity (> 95%) and a high specificity (> 98%). The positive rates of the 81,000 antigen, the 38,000 antigen and the CF-10 antigen in tuberculosis patients were 64.4% (96/149), 57.0% (85/149) and 37.6% (56/149) respectively. The negative rates in nontuberculosis patient were all above 98%. The positive rate of the 3 antigens combined was 81.2% (121/149) and the negative rate was 98%.
  
  
  CONCLUSIONS
  The specificity of the 3 antigens was high. Sensitivity and specificity of the 3 antigens combined was better than each antigen alone. It is clinically practical that detection of multiple antigens may be used to improve the serodiagnosis of Mycobacterium tuberculosis.
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
authors.forename: En-lan
authors.initials: EL
authors.lastname: Wang
authors.forename: Wen-tao
authors.initials: WT
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000097:blood* / Q000145:classification; D006801:Humans; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D013183:Sputum; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000276:immunology; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial