[Determination of metacycline in mixture sample by synchronous-derivative fluorimetry].
A new synchronous-derivative fluorimetric method for the determination of metacycline (MTC) in the presence of oxytetracycline (OTC) is proposed. The degradation products of MTC in 0.1 mol x L(-1) NaOH are strongly fluorescent. The addition of cetyltrimethylammonium bromide (CTMAB) enhances the fluorescence intensity 2-fold. The synchronous fluorescence spectra of MTC and OTC degradation products do not interfere with each other under the condition that deltalambda = 100 nm. MTC can be determined in the presence of OTC in the range of OTC+MTC ratio of 1+116-1+1. The recoveries of MTC in the mixture sample are in the range of 94%-102%.
Aodeng
Gao-Wa
GW
College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, China.
Zhang
Ying
Y
Fan
Hai-Yan
HY
D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D008690:Methacycline / Q000032:analysis* / Q000737:chemistry; D010118:Oxytetracycline / Q000737:chemistry*; D015203:Reproducibility of Results; D012972:Sodium Hydroxide / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*
D002593:Cetrimonium Compounds; D012972:Sodium Hydroxide; D008690:Methacycline; D010118:Oxytetracycline; D000077286:Cetrimonium