Interacting partners of M-PMV nucleocapsid-dUTPase.
The nucleocapsid-dUTPase protein of Mason-Pfizer monkey virus is a truly bifunctional fusion enzyme. The exact role of this fusion protein in the viral life cycle is unclear. To explore its function, we started to identify interacting protein partners of the enzyme in vitro. Three viral proteins, integrase, capsid and nucleocapsid, were found to be capable of physical interaction with NC-dUTPase. Integrase protein is an important component within the preintegration complex; therefore the present results also suggest that NC-dUTPase might be associated with this complex.
Németh-Pongrácz
V
V
Institute of Enzymology, Hungarian Academy of Sciences, Budapest, Hungary. pongracz@enzim.hu
Snasel
J
J
Rumlova
M
M
Pichova
I
I
Vértessy
B G
BG
D036022:Capsid Proteins; D019426:Integrases / Q000737:chemistry; D007700:Kinetics; D016093:Mason-Pfizer monkey virus / Q000201:enzymology*; D019251:Nucleocapsid / Q000737:chemistry; D019590:Nucleocapsid Proteins / Q000737:chemistry*; D011485:Protein Binding; D011755:Pyrophosphatases / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013997:Time Factors; D019065:Virus Assembly; D016662:Virus Integration
D036022:Capsid Proteins; D019590:Nucleocapsid Proteins; D011993:Recombinant Fusion Proteins; D019426:Integrases; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase