title: Quadruple antigenic epitope peptide producing immune protection against classical swine fever virus.
abstract: Research on epitope-based vaccines is a current focus in the development of new vaccines against classical swine fever virus (CSFV). The present study aimed to engineer a quadruple antigenic epitope peptide of the CSFV immunogen E2 glycoprotein by splice overlap extension (SOE) PCR, expressed in E. coli fused with glutathione S-transferase (GST), and named rGST-4E. Enzyme-linked immunosorbent assay (ELISA) and Western blot analysis showed that purified rGST-4E had an excellent immunoreactivity with swine anti-CSFV serum and rabbit anti-E2 serum. Animal vaccination trials showed that the rGST-4E was more immunogenic than mono-epitope peptide and was able to produce effective immune protection in rabbits against challenge with hog cholera lapinized virus, and in pigs against challenge with virulent CSFV. These data show that the recombinant repeated epitope peptide could be considered a potential epitope-based vaccine for prevention of the disease.
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun 130062, China.
authors.forename: Siguo
authors.initials: S
authors.lastname: Liu
authors.forename: Xinglong
authors.initials: X
authors.lastname: Yu
authors.forename: Chunlai
authors.initials: C
authors.lastname: Wang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wu
authors.forename: Xiangang
authors.initials: X
authors.lastname: Kong
authors.forename: Changchun
authors.initials: C
authors.lastname: Tu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis; D000917:Antibody Formation / Q000276:immunology; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D006691:Classical Swine Fever / Q000276:immunology* / Q000517:prevention & control*; D006692:Classical Swine Fever Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005982:Glutathione Transferase / Q000276:immunology; D011817:Rabbits; D013552:Swine; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D014614:Vaccines, Synthetic; D005982:Glutathione Transferase