title: Quantitative analysis of cytochrome C released from rice mitochondria using the adsorptive polarographic wave of guanidine modified Co(II)-cytochrome C complex.
abstract: A new, simple and sensitive method for the quantitative analysis of cytochrome C (Cyt C) based on the reduction wave of guanidine modified Co(II)-Cyt C complex at about -1.74 V (vs. SCE) by single sweep polarography in the solution containing 8 x 10(-6) mol L(-1) CoCl2, 0.04 mol L(-1) guanidine hydrochloride, 0.2 mol L(-1) NaOH and 0.5% Na2SO3. The peak height is linearly proportional to the concentration of Cyt C in the range of 0.005 approximately 1.500 mg L(-1) (correlation coefficient 0.999). Common amino acids, saccharide, organic acid and metal ions of appropriate concentrations have no interference on the Cyt C determination. The released Cyt C in the process of mitochondrial permeability transition of Hong-Lian cytoplasmic male sterile line of rice has been measured by the method, and the result is satisfactory.
authors.affiliation: Key Lab for Biotechnology of National Commission for Nationalities, South-Central University for Nationalities, Wuhan 430074, P R China.
authors.forename: Chunjiao
authors.initials: C
authors.lastname: Xia
authors.forename: Xuequn
authors.initials: X
authors.lastname: Liu
authors.forename: Dengbai
authors.initials: D
authors.lastname: Luo
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Wang
authors.forename: Fengqin
authors.initials: F
authors.lastname: He
authors.forename: Lei
authors.initials: L
authors.lastname: Yuan
authors.forename: Chuntai
authors.initials: C
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry; D045304:Cytochromes c / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D019791:Guanidine / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism*; D009005:Monosaccharides / Q000737:chemistry; D012275:Oryza / Q000378:metabolism*; D011048:Polarography / Q000379:methods*; D015203:Reproducibility of Results; D012972:Sodium Hydroxide / Q000737:chemistry; D013447:Sulfites / Q000737:chemistry
chemical_list: D000596:Amino Acids; D009005:Monosaccharides; D013447:Sulfites; D003035:Cobalt; D012972:Sodium Hydroxide; D045304:Cytochromes c; C018021:cobaltous chloride; D019791:Guanidine; C025026:sodium sulfite