title: Promoter of the heat shock testis-specific Hsp70.2/Hst70 gene is active in nervous system during embryonic development of mice.
abstract: The Hsp70.2/Hst70 gene is a unique member of the 70 kDa heat shock proteins multigene family whose activity is regulated developmentally; in adult mice and rats its expression is restricted mostly to meiotic and postmeiotic male germ cells. In aim to analyze activity of the Hsp70.2/Hst70 promoter in developing embryos we have constructed transgenic mice expressing EGFP reporter gene under control of the rat Hst70 promoter. The appearance of EGFP fluorescence coincides with series of major developmental events, such as extra-embryonic membranes formation, axial rotation, formation of neural tube and the primordium of central nervous system, formation of differentiated somites, extensive remodeling of the heart, development of fingers and toes, and sensory organs formation. Activity of the Hst70 promoter localizes mostly inside nervous system indicating the role of Hsp70.2/Hst70 gene in development of this system.
authors.affiliation: Department of Histology and Embryology, Silesian University, 9 Bankowa Str., 40-007, Katowice, Poland. rupik@us.edu.pl
authors.forename: Weronika
authors.initials: W
authors.lastname: Rupik
authors.forename: Agata
authors.initials: A
authors.lastname: Stawierej
authors.forename: Iwona
authors.initials: I
authors.lastname: Stolarczyk
authors.forename: Wieslawa
authors.initials: W
authors.lastname: Widłak
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009420:Nervous System / Q000196:embryology* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000502:physiology*; D013737:Testis / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins