title: Polymorphism of gp15/400 allergen gene of Wuchereria bancrofti from different regions of India endemic for lymphatic filariasis.
abstract: Nematode polyprotein allergens (NPA) are lipid-binding/transport molecules that elicit elevated levels of IgE response in the infected host, leading to Th2 type of immune response. They also transport arachidonic acid and metabolites that is known to be the action of antifilarial drug, diethylcarbamazine, and hence are of great significance to the control of lymphatic filariasis. We investigated the polymorphism of gp15/400 polyprotein of 35 isolates of lymphatic filarial parasite Wuchereria bancrofti collected from different geographic locations of India. The repeat subunit of the gene was found to be highly conserved in all the isolates with only two nucleotide synonymous changes at positions 369 (A-G) and 375 (C-T). As this molecule is highly conserved and has multifarious roles in the survival and pathogenesis of the parasite, it has good potential as a target for drug, immunodulation tools and immunotherapy development.
authors.affiliation: Molecular Biology and Bioinformatics Unit, Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India. slhoti@yahoo.com
authors.forename: S L
authors.initials: SL
authors.lastname: Hoti
authors.forename: R
authors.initials: R
authors.lastname: Thangadurai
authors.forename: K P
authors.initials: KP
authors.lastname: Patra
authors.forename: P K
authors.initials: PK
authors.lastname: Das
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D004605:Elephantiasis, Filarial / Q000453:epidemiology / Q000276:immunology / Q000469:parasitology*; D019353:Endemic Diseases; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics*; D007194:India / Q000453:epidemiology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D014958:Wuchereria bancrofti / Q000235:genetics* / Q000276:immunology
chemical_list: D000485:Allergens; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C520090:GP15-400 protein, Wuchereria bancrofti; D015801:Helminth Proteins; D008562:Membrane Glycoproteins