[Study on quality for Herba Polygoni Orientalis].
OBJECTIVE
To establish the qualitative and quantitative detective methods of Herba Polygoni Orientalis.


METHODS
Isorientin, orientin, protocatechuic acid and gallic acid in Herba Polygoni Orientalis were identified by TLC. The contents of isorientin and orientin in Herba Polygoni Orientalis were determined by HPLC.


RESULTS
Isorientin, orientin, protocatechuic acid and gallic acid could be identified by TLC. Isorientin and orientin were well separated with Diamonsil C18 column and acetonitrile-0.1% phosphoric acid (18:82) as mobile phase. The linear range of isorientin was 0.075-0.90 microg. The average recovery of isorientin was 98.8% and RSD was 2.1%. The linear range of orientin was 0.041-0.49 microg. The average recovery of orientin was 98.8% and RSD was 2.1%.


CONCLUSIONS
The methods can be used for qualitative identification and quantitation determination of Herba Polygoni Orientalis.
Wang
Ai-min
AM
School of Pharmacy, Guiyang Medical Colledge, China.
Wang
Yong-lin
YL
Hu
Li-na
LN
Li
Yong-jun
YJ
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005419:Flavonoids / Q000032:analysis*; D005707:Gallic Acid / Q000032:analysis; D005960:Glucosides / Q000032:analysis*; D062385:Hydroxybenzoates / Q000032:analysis; D047311:Luteolin / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D005419:Flavonoids; D005960:Glucosides; D062385:Hydroxybenzoates; C009091:protocatechuic acid; D005707:Gallic Acid; C057912:homoorientin; C065886:orientin; D047311:Luteolin