title: Instrumental method for automated on-line fraction analysis and peak deconvolution in multicomponent-overloaded high-performance liquid chromatography.
abstract: A bi-dimensional (non-orthogonal) chromatographic system made of two columns of different length (longer along the first direction and shorter on the second) but packed with identical (or similar) materials has been employed to perform automated on-line fraction analysis and peak deconvolution of multicomponent overloaded band profiles. Multicomponent overloaded peaks, eluted from the first column, are sampled through a remotely-controlled switching valve and fractions are injected, under analytical conditions, directly into the second direction. This set-up serves a twofold purpose. First of all, it has been possible to use linear calibration curves for detector calibration (DC). Secondly, since single component peaks were resolved on the second direction, individual concentration profiles have been obtained beneath the envelop of overlapping bands. All the information necessary to operate the bi-dimensional system can be achieved by analyzing the chromatographic behavior of the mixture on the shorter column under linear conditions. Therefore, solutions of unknown concentration can be prepared and their composition reconstructed with a simple chromatographic run. Two- and three-component overloaded mixtures have been employed to validate the system.
authors.affiliation: Department of Chemistry, University of Ferrara Via L. Borsari 46, 44100 Ferrara, Italy. cvz@unife.it <cvz@unife.it>
authors.forename: Alberto
authors.initials: A
authors.lastname: Cavazzini
authors.forename: Valentina
authors.initials: V
authors.lastname: Costa
authors.forename: Giorgio
authors.initials: G
authors.lastname: Nadalini
authors.forename: Francesco
authors.initials: F
authors.lastname: Dondi
mesh_terms: D001331:Automation; D019905:Benzyl Alcohol / Q000302:isolation & purification; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D009862:Online Systems / Q000295:instrumentation*; D019800:Phenol / Q000302:isolation & purification; D010626:Phenylethyl Alcohol / Q000302:isolation & purification
chemical_list: D019800:Phenol; D019905:Benzyl Alcohol; D010626:Phenylethyl Alcohol