title: Preparation of graded zirconia hydroxyapatite composite bioceramic and its immunocompatibility in vitro.
abstract: To obtain immunocompatibility, graded zirconia-hydroxyapatite (ZrO(2)-HA) composite bioceramic and simplex ZrO(2)-HA composite bioceramic are prepared. Peripheral blood mononuclear cells (PBMCs) harvested from healthy individuals are cultured with the two ceramic extracts to assess their effect on the transformation of lymphocytes, apoptotic ratio, CD69 expression, and expression of cytokine of TNFalpha and IL-6, with or without phytohemoagglutinin (PHA)-stimulated cells. Ceramic extracts did not activate the resting lymphocytes, whereas the response of the PHA-stimulated cells was significantly modified. The PBMCs activated by graded ZrO(2)-HA composite bioceramic is noticeably smaller than that by simplex ZrO(2)-HA composite bioceramic; these results, however can only be seen under the amplified effect of PHA-stimulation.
authors.affiliation: Department of Orthopaedics, Second Affiliated Hospital, Zhejiang University, 310009 Hangzhou, China.
authors.forename: Renfu
authors.initials: R
authors.lastname: Quan
authors.forename: Disheng
authors.initials: D
authors.lastname: Yang
authors.forename: Xudong
authors.initials: X
authors.lastname: Miao
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wu
authors.forename: Hongbin
authors.initials: H
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D017209:Apoptosis; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D002478:Cells, Cultured; D002516:Ceramics / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015040:Zirconium / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D001672:Biocompatible Materials; D017886:Durapatite; D015850:Interleukin-6; D037181:Lectins, C-Type; D014409:Tumor Necrosis Factor-alpha; D015040:Zirconium; D000099192:CD69 Antigens; C028541:zirconium oxide