A novel depolarizing activity of scorpion venom fraction M1 due to activation of skeletal muscle nicotinic receptors.
A depolarizing activity following interaction with nicotinic acetylcholine receptors (nAchRs) in skeletal muscle cells, was observed for the first time in the non-toxic venom fraction (M1) of the yellow scorpion Buthus occitanus tunetanus (Bot). The effects of M1 fraction were tested on cultured rat myotubes by recording changes in [Ca2+]i. When applied, M1 (10 microg/mL) induced a transient increase of [Ca2+]i which could be blocked by a prior application of alpha-Bungarotoxin (alpha-Bg-Tx).
Cheikh
Amani
A
Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, Tunis BP 74-1002, Tunisia.
Benkhalifa
Rym
R
Potreau
Daniel
D
Raymond
Guy
G
El Ayeb
Mohamed
M
Cognard
Christian
C
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects* / Q000378:metabolism; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology*; D051381:Rats; D011978:Receptors, Nicotinic / Q000187:drug effects* / Q000378:metabolism; D012604:Scorpion Venoms / Q000494:pharmacology*; D012605:Scorpions
D015220:Calcium Channels; D011978:Receptors, Nicotinic; D012604:Scorpion Venoms