Proteomic analysis of bacterial-blight defense-responsive proteins in rice leaf blades.
Plants exhibit resistance against incompatible pathogens, via localized and systemic responses as part of an integrated defense mechanism. To study the compatible and incompatible interactions between rice and bacteria, a proteomic approach was applied. Rice cv. Java 14 seedlings were inoculated with compatible (Xo7435) and incompatible (T7174) races of Xanthomonas oryzae pv. oryzae (Xoo). Cytosolic and membrane proteins were fractionated from the leaf blades and separated by 2-D PAGE. From 366 proteins analyzed, 20 were differentially expressed in response to bacterial inoculation. These proteins were categorized into classes related to energy (30%), metabolism (20%), and defense (20%). Among the 20 proteins, ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (RuBisCO LSU) was fragmented into two smaller proteins by T7174 and Xo7435 inoculation. Treatment with jasmonic acid (JA), a signaling molecule in plant defense responses, changed the level of protein accumulation for 5 of the 20 proteins. Thaumatin-like protein and probenazole-inducible protein (PBZ) were commonly up-regulated by T7174 and Xo7435 inoculation and JA treatment. These results suggest that synthesis of the defense-related thaumatin-like protein and PBZ are stimulated by JA in the defense response pathway of rice against bacterial blight.
Mahmood
Tariq
T
National Institute of Crop Science, Tsukuba, Japan.
Jan
Asad
A
Kakishima
Makoto
M
Komatsu
Setsuko
S
D016000:Cluster Analysis; D003517:Cyclopentanes; D015180:Electrophoresis, Gel, Two-Dimensional; D012275:Oryza / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D054883:Oxylipins; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D010940:Plant Proteins / Q000145:classification / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013844:Thiazoles; D015854:Up-Regulation; D014974:Xanthomonas / Q000472:pathogenicity*
D003517:Cyclopentanes; D054883:Oxylipins; C099062:PBZ1 protein, Oryza sativa; D010940:Plant Proteins; D013844:Thiazoles; C099063:oryzemate; C003427:thaumatin protein, plant; C011006:jasmonic acid