title: Study on inactivation kinetics of hepatitis A virus and enteroviruses with peracetic acid and chlorine. New ICC/PCR method to assess disinfection effectiveness.
abstract: The virucidal activity of chlorine-compounds was studied using hepatitis A virus (HAV) and Poliovirus 2 and comparing the disinfectant efficiency of peracetic acid. HAV presented a higher resistance to HClO than Poliovirus did. With ClO2 the inactivation times of HAV were markedly shorter. A comparison between these data and those resulting from the kinetics with peracetic acid (PA) showed that PA is less effective than chlorine. As a preliminary to future research, the PCR-test integrated with cell-cultures was experimentally introduced for a quick evaluation of the HAV-infectiveness, with the aim of possible application in the field of disinfection and of viruses-isolation from environmental and food samples.
authors.affiliation: Department of Public Health, Sec of Hygiene, University of Parma, Italy. laura.bigliardi@unipr.it
authors.forename: L
authors.initials: L
authors.lastname: Bigliardi
authors.forename: G
authors.initials: G
authors.lastname: Sansebastiano
mesh_terms: D018929:Cell Culture Techniques; D017606:Chlorine Compounds / Q000493:pharmacokinetics / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D024882:Drug Resistance, Viral; D029822:Enterovirus B, Human / Q000187:drug effects; D030041:Hepatitis A virus / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D006997:Hypochlorous Acid / Q000493:pharmacokinetics / Q000494:pharmacology; D016014:Linear Models; D016015:Logistic Models; D010087:Oxides / Q000493:pharmacokinetics / Q000494:pharmacology; D010463:Peracetic Acid / Q000494:pharmacology; D017955:Poliovirus / Q000187:drug effects*; D016133:Polymerase Chain Reaction; D038301:Virus Inactivation / Q000187:drug effects*
chemical_list: D017606:Chlorine Compounds; D004202:Disinfectants; D010087:Oxides; D006997:Hypochlorous Acid; C025109:chlorine dioxide; D010463:Peracetic Acid