title: Cyclooxygenases in rat Leydig cells: effects of luteinizing hormone and aging.
abstract: Previous studies suggested that increased Leydig cell cyclooxygenase (COX)2 expression may be involved in the reduced testosterone production that characterizes aged Leydig cells. Our objective herein was to further elucidate the relationships among LH stimulation, Leydig cell COX2 and COX1 expression, aging, and testosterone production. Incubation of Leydig cells from young or aged rats with LH or dibutyryl cAMP resulted in increases in both intracellular COX2 protein expression and testosterone production. COX1 expression did not respond to LH or dibutyryl cAMP. Incubation of adult cells with a protein kinase A inhibitor suppressed the stimulatory effects of LH on COX2 and testosterone production. Short-term incubation of Leydig cells with TGF-alpha or IL-1beta also increased COX2 protein levels; IGF-I had no effect. In vivo, LH also was found to stimulate both COX2 and testosterone, but not COX1. As reported previously, COX2 expression was greater in old than in young cells, and old Leydig cells responded to inhibition of COX2 in vitro with increased testosterone production. However, the effects of the COX2 inhibitors were not restricted to old cells; young Leydig cells also responded to COX2 inhibition with increased testosterone production. This and the observation that the incubation of young or old cells with LH resulted in increased COX2 and testosterone production in both cases suggests that the relationship between COX2 and testosterone production is not unique to aged Leydig cells. Moreover, the close correlation between increases in COX2 and testosterone in LH-stimulated young and aged Leydig cells is difficult to reconcile with the contention that the increased expression of COX2 in aged cells is responsible for age-related suppression of Leydig cell testosterone production.
authors.affiliation: Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins University Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, Maryland 21205, USA. hchen@jhsph.edu
authors.forename: Haolin
authors.initials: H
authors.lastname: Chen
authors.forename: Lindi
authors.initials: L
authors.lastname: Luo
authors.forename: June
authors.initials: J
authors.lastname: Liu
authors.forename: Barry R
authors.initials: BR
authors.lastname: Zirkin
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D051545:Cyclooxygenase 1 / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D053583:Interleukin-1beta / Q000494:pharmacology; D007985:Leydig Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D037101:Luteinizing Hormone, beta Subunit / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D013739:Testosterone / Q000096:biosynthesis; D016211:Transforming Growth Factor alpha / Q000494:pharmacology
chemical_list: D016861:Cyclooxygenase Inhibitors; D053583:Interleukin-1beta; D037101:Luteinizing Hormone, beta Subunit; D016211:Transforming Growth Factor alpha; D013739:Testosterone; D003994:Bucladesine; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2