title: Structural and functional analysis of natrin, a venom protein that targets various ion channels.
abstract: Cysteine-rich secretory proteins (CRISPs) are secreted single-chain proteins found in different sources. Natrin is a member of the CRISP family purified from the snake venom of Naja naja atra, which has been reported as a BKca channel blocker. In our study, crystals of natrin were obtained in two different crystal forms and the structure of one of them was solved at a resolution of 1.68A. Our electrophysiological experiments indicated that natrin can block the ion channel currents of the voltage-gated potassium channel Kv1.3. Docking analyses of the interaction between natrin and Kv1.3 revealed a novel interaction pattern different from the two previously reported K(+) channel inhibition models termed "functional dyad" and "basic ring". These findings offered new insights into the function of natrin and how the specific interactions between CRISPs and different ion channels can be achieved.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.forename: Ming-na
authors.initials: MN
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Song
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Han
authors.forename: Qiong-ling
authors.initials: QL
authors.lastname: Wang
authors.forename: Chang-chen
authors.initials: CC
authors.lastname: Yin
authors.forename: Yuan-cong
authors.initials: YC
authors.lastname: Zhou
authors.forename: Zhi
authors.initials: Z
authors.lastname: Qi
authors.forename: Yu-yan
authors.initials: YY
authors.lastname: Shu
authors.forename: Zheng-jiong
authors.initials: ZJ
authors.lastname: Lin
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D018360:Crystallography, X-Ray; D004546:Elapid Venoms / Q000737:chemistry* / Q000378:metabolism; D051664:Kv1.3 Potassium Channel / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: D004546:Elapid Venoms; D051664:Kv1.3 Potassium Channel; C495581:natrin protein, Naja atra