Sequence analysis of the rfb loci, encoding proteins involved in the biosynthesis of the Salmonella enterica O17 and O18 antigens: serogroup-specific identification by PCR.
We report sequencing of the O antigen encoded by the rfb gene cluster of Salmonella enterica serotype Jangwani (O17) and Salmonella serotype Cerro (O18). We developed serogroup O17- and O18-specific PCR assays based on rfb gene targets and found them to be sensitive and specific for rapid identification of Salmonella serogroups O17 and O18.
Fitzgerald
Collette
C
Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30333, USA. chf3@cdc.gov
Gheesling
Linda
L
Collins
Marcus
M
Fields
Patricia I
PI
D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013045:Species Specificity
D001426:Bacterial Proteins; D019081:O Antigens; C079397:rfb protein, Bacteria