Species-specific SCAR markers for authentication of Sinocalycanthus chinensis.
Sinocalycanthus chinensis, an endangered species endemic to China, is cultivated as an ornamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthus floridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR (inter-simple sequence repeats) were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR (sequence characterized amplified regions) markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs of S. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid (results can be obtained in less than 3 h), but is also easy to perform. Hence it is a feasible method for identifying S. chinensis in seedling market.
Ye
Qian
Q
Laboratory of Systematic and Evolutionary Botany and Biodiversity, School of Life Sciences, Zhejiang University, Hangzhou 310029, China.
Qiu
Ying-xiong
YX
Quo
Yan-qi
YQ
Chen
Jian-xin
JX
Yang
Shu-zhen
SZ
Zhao
Ming-shui
MS
Fu
Cheng-xin
CX
D031237:Calycanthaceae / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity
D018744:DNA, Plant; D005819:Genetic Markers