Mediating electron transfer from bacteria to a gold electrode via a self-assembled monolayer.
Numerous bacterial genera are known to respire anaerobically using macroscopic electrodes as electron acceptors. Typically, inexpensive graphite electrodes, which are readily colonized, are used to monitor electrogenic bacterial metabolism for microbial fuel cell and bioelectronics studies. We compare current production by electrogenic bacteria on gold electrodes coated with various alkanethiol self-assembled monolayers to current production on glassy carbon electrodes. Current production is correlated to chain length and headgroup of the monolayer molecules as expected. Relative to graphite, the coated gold electrodes achieve more reproducible experimental conditions and certain headgroups enhance electronic coupling to the bacteria.
Crittenden
Scott R
SR
US Army Research Laboratory, Sensors and Electron Devices Directorate, Adelphi, Maryland 20783, USA.
Sund
Christian J
CJ
Sumner
James J
JJ
D004566:Electrodes; D004583:Electrons; D006046:Gold / Q000737:chemistry*; D020614:Shewanella putrefaciens / Q000737:chemistry*
D006046:Gold