title: A label-free protein microfluidic array for parallel immunoassays.
abstract: A label-free protein microfluidic array for immunoassays based on the combination of imaging ellipsometry and an integrated microfluidic system is presented. Proteins can be patterned homogeneously on substrate in array format by the microfluidic system simultaneously. After preparation, the protein array can be packed in the microfluidic system which is full of buffer so that proteins are not exposed to denaturing conditions. With simple microfluidic channel junction, the protein microfluidic array can be used in serial or parallel format to analyze single or multiple samples simultaneously. Imaging ellipsometry is used for the protein array reading with a label-free format. The biological and medical applications of the label-free protein microfluidic array are demonstrated by screening for antibody-antigen interactions, measuring the concentration of the protein solution and detecting five markers of hepatitis B.
authors.affiliation: Institute of Mechanics, Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Zhan-Hui
authors.initials: ZH
authors.lastname: Wang
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Meng
authors.forename: Pei-Qing
authors.initials: PQ
authors.lastname: Ying
authors.forename: Cai
authors.initials: C
authors.lastname: Qi
authors.forename: Gang
authors.initials: G
authors.lastname: Jin
mesh_terms: D000818:Animals; D006510:Hepatitis B Antibodies / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006513:Hepatitis B e Antigens / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*; D012709:Serum Albumin / Q000032:analysis
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D007074:Immunoglobulin G; D012709:Serum Albumin