title: Gingival crevicular fluid matrix metalloproteinase-13 levels and molecular forms in various types of periodontal diseases.
abstract: BACKGROUND
  The purpose of this study was to evaluate the levels, molecular forms and activation degree of matrix metalloproteinase-13 (MMP-13) in the gingival crevicular fluid (GCF) of patients with periodontal diseases and to correlate these findings with periodontal clinical parameters.
  
  
  METHODS
  Sixty one subjects participated in this study as healthy (n = 18), gingivitis (n = 17), aggressive periodontitis (AgP; n = 15) and chronic periodontitis (CP; n = 11) groups. Clinical measurements and GCF samples were obtained from each subject. The molecular forms of MMP-13 in GCF samples were analyzed by Western immunoblotting method. Differences among the groups were assessed using non-parametric statistical analysis.
  
  
  RESULTS
  In the CP group, levels of 29-30 kDa fragment of MMP-13, total MMP-13, and activated form of MMP-13 were significantly higher than in the healthy, gingivitis and AgP groups. GCF levels of all molecular forms of MMP-13 in AgP group were similar to those of healthy and gingivitis groups. Total and activated MMP-13 levels were positively correlated with all clinical parameters. 29-30 kDa fragment levels of MMP-13 were also positively correlated with papillary bleeding index and plaque index.
  
  
  CONCLUSIONS
  These results indicate that elevated GCF MMP-13 levels may play an important role in the pathogenesis of CP. These data demonstrate, for the first time, pathologically activated and elevated MMP-13 in GCF.
authors.affiliation: Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Ilgenli
authors.forename: S
authors.initials: S
authors.lastname: Vardar-Sengul
authors.forename: A
authors.initials: A
authors.lastname: Gürkan
authors.forename: T
authors.initials: T
authors.lastname: Sorsa
authors.forename: S
authors.initials: S
authors.lastname: Stackelberg
authors.forename: T
authors.initials: T
authors.lastname: Köse
authors.forename: G
authors.initials: G
authors.lastname: Atilla
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D015153:Blotting, Western; D016022:Case-Control Studies; D002908:Chronic Disease; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000032:analysis; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D005891:Gingivitis / Q000201:enzymology*; D006801:Humans; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008875:Middle Aged; D008970:Molecular Weight; D010518:Periodontitis / Q000201:enzymology*
chemical_list: D004792:Enzyme Precursors; D053509:Matrix Metalloproteinase 13