Immunolocalization of E-cadherin and alphaE-catenin in rat parotid acinar cell under chronic stimulation of isoproterenol.
E-cadherin and alphaE-catenin were localized in normal and chronically isoproterenol-treated acinar cells of rat parotid gland by means of immunogold labelling of Lowicryl embedded sections. Immunostaining of both experimental groups with polyclonal antibodies to E-cadherin and alphaE-catenin was mainly restricted to the areas of adherens junctions. Surprisingly, in isoproterenol-treated cell alphaE-catenin was also found on the secretory granules periphery and appeared to encircle a secretory vesicle. In isoproterenol-induced cell hyperproliferation, the maintened presence of adherens junctions components, such as E-cadherin and alphaE-catenin molecules, should be an essential prerequisite for tissue integrity. Our data suggest the presence of a correlation between the organization of actin and the localization of alphaE-catenin in the chronically isoproterenol-treated acinar cell of rat parotid gland.
D'Amico
Fabio
F
Department of Biomedical Sciences, University of Catania, Catania, Italy. f.damico@unict.it
Skarmoutsou
Evangelia
E
D000199:Actins / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D010306:Parotid Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051178:alpha Catenin / Q000378:metabolism*
D000199:Actins; D000318:Adrenergic beta-Agonists; D015820:Cadherins; D051178:alpha Catenin; D007545:Isoproterenol