title: [Antivirus effects of extract from gardenia].
abstract: OBJECTIVE
  To observe the effect of the extract from gardenia on influenza viral pneumonia in mice and virus-induced cytopathic effect.
  
  
  METHODS
  The mice were infected by influenza virus in nasal, the lung inflammation, mortality rate and life elongation rate were observed respectively. The anti-viral activity of the extract from gardenia was accessed by cytopathic effect (CPE) in vitro and 0% toxicity concentration (TC0), 50% toxicity concentration (TC50), 50% inhibitor concentration (IC50), therapeutic index (TI) were determined by Reed-Muench method.
  
  
  RESULTS
  The pneumonia induced by influenza virus in mice was inhibited significantly by the extract from gardenia, as the mortality rate decreased and the life elongation rate increased remarkably. Meanwhile the NO content in serum decreased significantly; The cytopathic effect induced by six kinds of viruses was inhibited remarkably.
  
  
  CONCLUSIONS
  The six kinds of viruses were inhibited significantly by the extract from gardenia which inhibitory effect on mice influenza viral pneumonia was related to the NO content decreased.
authors.affiliation: Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Belting 100700, China.
authors.forename: Yi-Zhong
authors.initials: YZ
authors.lastname: Wang
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Cui
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Gao
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Guo
authors.forename: Xiu-Kun
authors.initials: XK
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Huang
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhao
authors.forename: Weng-Feng
authors.initials: WF
authors.lastname: Gong
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000821:virology; D004947:Esophagus / Q000166:cytology / Q000821:virology; D005260:Female; D032062:Gardenia / Q000737:chemistry; D018259:Herpesvirus 1, Human / Q000187:drug effects; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000097:blood; D009975:Orthomyxoviridae / Q000472:pathogenicity; D010946:Plants, Medicinal / Q000737:chemistry; D011024:Pneumonia, Viral / Q000097:blood / Q000188:drug therapy*; D011897:Random Allocation; D018113:Respiratory Syncytial Virus, Human / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D009569:Nitric Oxide