Quantitative structure analysis of genetic diversity among spring bread wheats (Triticum aestivum L.) from different geographical regions.
Genetic diversity in spring bread wheat (T. aestivum L.) was studied in a total of 69 accessions. For this purpose, 52 microsatellite (SSR) markers were used and a total of 406 alleles were detected, of which 182 (44.8%) occurred at a frequency of <5% (rare alleles). The number of alleles per locus ranged from 2 to 14 with an average of 7.81. The largest number of alleles per locus occurred in the B genome (8.65) as compared to the A (8.43) and D (5.93) genomes, respectively. The polymorphism index content (PIC) value varied from 0.24 to 0.89 with an average of 0.68. The highest PIC for all accessions was found in the B genome (0.71) as compared to the A (0.68) and D genomes (0.63). Genetic distance-based method (standard UPGMA clustering) and a model-based method (structure analysis) were used for cluster analysis. The two methods led to analogical results. Analysis of molecular variance (AMOVA) showed that 80.6% of the total variation could be explained by the variance within the geographical groups. In comparison to the diversity detected for all accessions (H ( e ) = 0.68), genetic diversity among European spring bread wheats was H ( e ) = 0.65. A comparatively higher diversity was observed between wheat varieties from Southern European countries (Austria/Switzerland, Portugal/Spain) corresponding to those from other regions.
Hai
Lin
L
Department of Plant Breeding, Research Centre for Biosystems, Land Use and Nutrition, Justus-Liebig University of Giessen, Heinrich-Buff-Ring 26-32, D-35392, Giessen, Germany.
Wagner
Carola
C
Friedt
Wolfgang
W
D000483:Alleles; D001939:Bread; D032461:Chromosomes, Plant; D014644:Genetic Variation; D005823:Genetics; D018745:Genome, Plant; D018895:Microsatellite Repeats; D008957:Models, Genetic; D015233:Models, Statistical; D010802:Phylogeny; D011110:Polymorphism, Genetic; D014908:Triticum / Q000235:genetics*