[Determination of cyclovirobuxine-D in huangyangning tablets by RP-HPLC with gradient elution].
OBJECTIVE
To establish a new method for the determination of cyclovirobuxine-D in huangyangning tablets.


METHODS
Agilent Zorbax Extend C18 (4.6 mm x 250 mm, 5 microm) column with temperature 40 degrees C was used. The mobile phase was 0.3% diethylamine methanol solution and 0.3% diethylamine water solution, with a linear gradient of 0.3% diethylamine methanol solution from 78% to 95% in 9 minutes, and then maintained for 6 minutes. The flow rate was 0.8 mL x min(-1). Evaporative light scattering detector was used.


RESULTS
The calibration curve was linear at a range of 0.57-11.44 microg for the cyclovirobuxine D (r = 0.9996). The average recovery was 97.2% and RSD was 1.1% (n = 9).


CONCLUSIONS
This method is rapid, simple, and reproducible, and can be used as a quality control method for this preparation.
Chen
Yong
Y
Zhejiang Institute for Drug Control, Hangzhou 310004, China. zhouzhou0311@126.com
Fan
Xue-Jun
XJ
Gong
Qing
Q
D031235:Buxus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D013607:Tablets
D004365:Drugs, Chinese Herbal; D013607:Tablets; C032939:cyclovirobuxine D