title: [Experimental studies on hepatotoxicity of rats induced by Fructus Gardeniae].
abstract: OBJECTIVE
  To study the hepatotoxicity effects in rats with different extract of Fructus Gardeniae.
  
  
  METHODS
  Observe the change of appearance, behavior and weight of rats through oral gavage daily for 3 d. Weigh the liver and calculate the liver index. Detect the ALT, AST and TBIL. Observe the liver tissue by optical microscope.
  
  
  RESULTS
  The weight and index of liver were increased by 3.08 g x kg(-1) aqueous extract, 1.62 g x kg(-1) alcoholic extract and 0.28 g x kg(-1) geniposide, compared to those of the blank group (P < 0.005, P < 0.001) and the activities of ALT, AST and the content of TBIL were also increased, compared to those of the blank group (P < 0.05, P < 0.001). The liver cells were obviously swell, necrotic and changed with inflammatory infiltrate.
  
  
  CONCLUSIONS
  Aqueous extract, alcoholic extract and geniposide displayed hepatotoxicity, and the geniposide which was the main substance of the Fructus Gardeniae might be mainly responsible for the hepatotoxicity.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing.
authors.forename: Hong-jun
authors.initials: HJ
authors.lastname: Yang
authors.forename: Mei-hong
authors.initials: MH
authors.lastname: Fu
authors.forename: Zi-lun
authors.initials: ZL
authors.lastname: Wu
authors.forename: Ri-xing
authors.initials: RX
authors.lastname: Liang
authors.forename: Lu-qi
authors.initials: LQ
authors.lastname: Huang
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.forename: Geng
authors.initials: G
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000633:toxicity*; D005260:Female; D005638:Fruit / Q000737:chemistry; D032062:Gardenia / Q000737:chemistry; D039823:Iridoids / Q000302:isolation & purification / Q000633:toxicity*; D008099:Liver / Q000473:pathology*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D011714:Pyrans / Q000302:isolation & purification / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D039823:Iridoids; D011714:Pyrans; C007835:geniposide; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin