Nasal augmentation using injectable alginate and mesenchymal stem cells in the rabbit.
BACKGROUND
The goal of this study was to evaluate the use of the autogenous mesenchymal stem cells (MSCs) impregnated in an injectable alginate gel containing platelet-rich plasma (PRP) for nasal augmentation in rabbit model.


METHODS
Bone marrow-derived MSCs were isolated and expanded from New Zealand white rabbits. At confluence, the cells were mixed with sodium alginate solution. PRP was prepared from the rabbits and it was immediately mixed into the alginate-cell mixture. The cell-PRP-alginate mix was injected into a subcutaneous nasal area. Eight weeks after injection changes in facial contour, newly formed nasal hump was analyzed and the amount of chondroitin sulfate in tissue was measured.


RESULTS
Augmented nasal dorsa maintained their original shape until harvest. Immunohistochemical staining revealed that the deposited matrix was composed of type II collagen and that it was distributed abundantly and widely in the connective tissue of the tissue generated. The amount of chondroitin sulfate (main component of the proteoglycan in cartilage) produced was significantly higher when MSCs and PRP-alginate were used.


CONCLUSIONS
Injectable PRP-alginate gel containing autologous mesenchymal stem cells may offer a useful means of facial soft tissue augmentation.
Park
Dong-Joon
DJ
Department of Otolaryngology-Head and Neck Surgery, Yonsei University Wonju College of Medicine, Korea. rhico@yonsei.ac.kr
Choi
Jin Hyun
JH
Kim
Yoo-Jung
YJ
Kim
Jin-Hyung
JH
Kim
Ki-Taek
KT
D000464:Alginates / Q000008:administration & dosage*; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage*; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000032:analysis / Q000096:biosynthesis; D024043:Collagen Type II / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005782:Gels; D007150:Immunohistochemistry; D007279:Injections, Subcutaneous; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009666:Nose / Q000601:surgery*; D053657:Platelet-Rich Plasma / Q000166:cytology; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D014182:Transplantation, Autologous; D016896:Treatment Outcome
D000464:Alginates; D001672:Biocompatible Materials; D024043:Collagen Type II; D005782:Gels; D002809:Chondroitin Sulfates