title: Assessment of 4-nitro-1,8-naphthalic anhydride reductase activity in homogenates of bakers' yeast by reversed-phase high-performance liquid chromatography.
abstract: A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous determination of yield and conversion ratio of 4-nitro-1,8-naphthalic anhydride to 4-amino-1,8-naphthalic anhydride following incubation with a crude bakers' yeast homogenate. The analytes were separated on a C18 column in gradient mode. The detection limit of 4-amino-1,8-naphthalic anhydride is 10ng/microl when using a 10microl sample injection volume. The nitroreductase activity in the homogenate system can be assessed during the bioconversion process. The method can be used for the simultaneous detection of 4-hydroxylamino-1,8-naphthalic anhydride, an intermediate with limited stability.
authors.affiliation: Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Xu
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Xuhong
authors.initials: X
authors.lastname: Qian
authors.forename: Jörgen
authors.initials: J
authors.lastname: Samuelsson
authors.forename: Jan-Christer
authors.initials: JC
authors.lastname: Janson
mesh_terms: D000812:Anhydrides / Q000138:chemical synthesis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D009601:Nitroreductases / Q000378:metabolism*; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000812:Anhydrides; D009601:Nitroreductases