Modification of the data-processing method for the turbidimetric bioassay of nisin.
The data processing method of the turbidimetric bioassay of nisin was modified to facilitate its industrial application. The influence of the initial indicator concentration was minimized by a redefined specific dose of the bacteriocin as the quotient between the titer of the added bacteriocin and the initial population density of the indicator in the suspension. It was found that dc = 0.125 microg ml(-1) was the critical dose of nisin that can cause a complete inhibition of the indicator, Pediococcus acidilactici UL5, with an initial OD of 0.135. To eliminate the interference of the cell debris, an equation, epsilonI = ODi(1-di)/OD0 = epsilonA(1-di/dc), exploiting dc, was formulated to obtain the intrinsic survival proportion. The use of the specific dose of the bacteriocin and the intrinsic survival proportion as parameters of the dose/response curve greatly enhanced its repeatability and feasibility. A dual-dosage approach was developed to further simplify the conventional standard dose/response curve method.
Wu
Zhaoliang
Z
Department of Bioengineering, Hebei University of Technology, Tianjin, People's Republic of China.
Li
Xueliang
X
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods*; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods; D016982:Micrococcus luteus / Q000187:drug effects* / Q000254:growth & development*; D008954:Models, Biological; D009561:Nisin / Q000494:pharmacology*; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods
D000900:Anti-Bacterial Agents; D009561:Nisin