Rapid determination of time-resolved fluoroimmunoassay for medroxyprogesterone acetate residues in pork tissues and comparison with liquid chromatography and tandem mass spectrometry.
A competitive time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of medroxyprogesterone acetate (MPA) residues in pork tissues. The limits of detection (LOD) was determined to be 0.06 ng g-1 and the limits of quantification (LOQ) was less than 0.8 ng g-1. The intra-assay variations were below 10% and the interassay variations ranged between 9.7 and 12.7%. The mean recoveries established at six concentration levels varied from 87.3 to 108.3%. The results obtained by the TR-FIA and ELISA showed a good correlation. The established TR-FIA was validated for the determination of incurred pork tissues and confirmed by high-performance liquid chromatography and tandem mass spectrometry (LC-MS-MS). This proposed technique could be applied to routine residue analysis.
Tieming
Huo
H
YanCheng Friend Biotechnology Corporation, 22100, YanCheng, JiangSu Province, China. tmh3@163.com
Chifang
Peng
P
Xiaogang
Chu
C
Chuanlai
Xu
X
D000818:Animals; D002853:Chromatography, Liquid; D003429:Cross Reactions; D015200:Fluoroimmunoassay / Q000379:methods*; D006728:Hormones / Q000032:analysis*; D017258:Medroxyprogesterone Acetate / Q000032:analysis*; D034421:Sus scrofa / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014018:Tissue Distribution
D006728:Hormones; D017258:Medroxyprogesterone Acetate