title: Uptake and biocompatibility of functionalized poly(vinylalcohol) coated superparamagnetic maghemite nanoparticles by synoviocytes in vitro.
abstract: Superparamagnetic iron oxide nanoparticles (SPION) were coated with either Polyvinyl alcohol (PVA) or Vinyl alcohol/vinyl amine copolymer and further functionalized with the fluorochromes Cy3.5 or Texas Red. A colloidally stable suspension of nanoparticles was incubated on sheep synovial cells in vitro for 3, 24, 72, and 120 hours. Nanoparticle internalization into synoviocytes as well as biocompatibility was visualized using light, fluorescence and confocal microscopy and fluorochrome labeled cells were quantified by flow cytometry. Data were analyzed by ANOVA factorial tests. Amino-PVA-SPION alone was detectable in cytoplasmic endosome-like structures after 3 hours of incubation but resulted in early cell death after 24 hours. Although amino-PVA-Cy3.5-SPION and PVA-TexasRed-SPION were taken up more slowly and less intensely, both labeled more than 80% of the cells in culture, but did not significantly change cell morphology or vitality at any time of evaluation in comparison to control cells. Results indicate that functionalized amino PVA-coated SPION are biocompatible, were successfully internalized by synoviocytes and hold promise for future biomedical applications utilizing magnetic drug targeting in joint disease.
authors.affiliation: Musculoskeletal Research Unit, Equine Hospital, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
authors.forename: Katja
authors.initials: K
authors.lastname: Schulze
authors.forename: Annette
authors.initials: A
authors.lastname: Koch
authors.forename: Alke
authors.initials: A
authors.lastname: Petri-Fink
authors.forename: Benedikt
authors.initials: B
authors.lastname: Steitz
authors.forename: Sarah
authors.initials: S
authors.lastname: Kamau
authors.forename: Michael
authors.initials: M
authors.lastname: Hottiger
authors.forename: Monika
authors.initials: M
authors.lastname: Hilbe
authors.forename: Lloyd
authors.initials: L
authors.lastname: Vaughan
authors.forename: Margarethe
authors.initials: M
authors.lastname: Hofmann
authors.forename: Heinrich
authors.initials: H
authors.lastname: Hofmann
authors.forename: Brigitte
authors.initials: B
authors.lastname: von Rechenberg
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems / Q000379:methods*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D008280:Magnetics; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011142:Polyvinyl Alcohol / Q000737:chemistry* / Q000494:pharmacology; D012756:Sheep; D013583:Synovial Membrane / Q000187:drug effects / Q000378:metabolism*
chemical_list: D020099:Coated Materials, Biocompatible; D011142:Polyvinyl Alcohol; D052203:Ferrosoferric Oxide