Experimental model to assess possible medicinal herb interaction with a radiobiocomplex: qualitative and quantitative analysis of kidney, liver and duodenum isolated from treated rats.
Ginkgo biloba extract (EGb) has been used as a medicinal herb. Several biological properties have been associated with this extract, especially, in the increase of the blood flow, in the action as platelet activating factor antagonism and in the prevention of the membrane against the damage caused by free radicals. Radiobiocomplexes have been utilized in various nuclear medicine procedures helping in the diagnosis and/or treatment of human diseases. Many substances have been reported to affect the bioavailability of different radiobiocomplexes. The aim of this work was to evaluate the possible influence of an EGb on the bioavailability of the sodium pertechnetate (99mTcO4Na) and on the morphometry of some organs isolated from rats. These animals were treated with EGb and 99mTcO4Na was injected. The animals were sacrificed, the organs isolated, counted in a well counter and the percentage of radioactivity per gram of each organ was calculated. The results showed that EGb decreased the uptake of the 99mTcO4Na in the duodenum (P<0.05). Moreover, morphometric analysis has revealed significant modifications (P<0.05) on kidney, liver and duodenum due to the cited treatment. It is speculated that the substances present in the EGb could act directly or generate metabolites capable to promote changes in organs (kidney, liver and duodenum), however, only significant alteration in the uptake of the 99mTcO4Na in the duodenum.
Moreno
S R F
SR
Programa de Pós-Graduação em Ciências Médicas, Universidade Federal Fluminense, Rua Marques de Parana 403, CEP 24030-210, Niterói, 24030-210, RJ, Brazil. srfmoreno@hotmail.com
Carvalho
J J
JJ
Nascimento
A L
AL
Pereira
M
M
Rocha
E K
EK
Olej
B
B
Caldas
L Q A
LQ
Bernardo-Filho
M
M
D000818:Animals; D001682:Biological Availability; D004347:Drug Interactions; D004386:Duodenum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005260:Female; D020441:Ginkgo biloba / Q000737:chemistry*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007678:Kidney Glomerulus / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D023421:Models, Animal; D010936:Plant Extracts / Q000633:toxicity*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D013670:Sodium Pertechnetate Tc 99m / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D010936:Plant Extracts; D019275:Radiopharmaceuticals; D013670:Sodium Pertechnetate Tc 99m