Antimalarial 4-phenylcoumarins from the stem bark of Hintonia latiflora.
The EtOAc extract of the stem bark of Hintonia latiflora showed the suppression of total parasitemia and the chemosuppression of schizont numbers, when tested in vivo against Plasmodium berghei infection in mice. Bioassay-directed fractionation of the EtOAc extract, using the in vitro 16 h and the in vivo 4-day suppression tests on P. berghei schizont numbers, led to the isolation of the new compound 5-O-beta-D-glucopyranosyl-7,4'-dimethoxy-3'-hydroxy-4-phenylcoumarin (1), along with the known 5-O-beta-D-glucopyranosyl-7-methoxy-3',4'-dihydroxy-4-phenylcoumarin (2). The structure of compound 1 was established on the basis of spectroscopic data interpretation. Compounds 1 and 2 suppressed the development of P. berghei schizonts in vitro with IC50 values of 24.7 and 25.9 microM, respectively. Compound 2 suppressed the development of schizonts at the dose of 40 mg/kg by 70.8% in the in vivo assay.
Argotte-Ramos
Rocío
R
Instituto Nacional de Salud Pública, Centro de Investigación sobre Enfermedades Infecciosas, Enfermedades Transmitidas por Vector, Departamento de Entomología Médica, Avenida Universidad 655, Cuernavaca, Morelos, México.
Ramírez-Avila
Guillermo
G
Rodríguez-Gutiérrez
María del Carmen
Mdel C
Ovilla-Muñoz
Marbella
M
Lanz-Mendoza
Humberto
H
Rodríguez
Mario H
MH
Gonzalez-Cortazar
Manasés
M
Alvarez
Laura
L
D000818:Animals; D000962:Antimalarials / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008800:Mexico; D051379:Mice; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D010962:Plasmodium berghei / Q000187:drug effects; D019666:Rubiaceae / Q000737:chemistry*
D000962:Antimalarials; D003374:Coumarins