title: Multipotent adult progenitor cell isolation and culture procedures.
abstract: We describe methods for isolation of multipotent adult progenitor cells (MAPCs) from newborn to 6-week-old mice and rats. The maintenance of these cells, including their culture, media formulas, and quality control procedures, are also explained. Additionally, ways to identify MAPCs including their phenotype and morphology are discussed.
authors.affiliation: University of Minnesota, Stem Cell Institute, Minneapolis, MN 55455, USA.
authors.forename: April
authors.initials: A
authors.lastname: Breyer
authors.forename: Nima
authors.initials: N
authors.lastname: Estharabadi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Oki
authors.forename: Fernando
authors.initials: F
authors.lastname: Ulloa
authors.forename: Molly
authors.initials: M
authors.lastname: Nelson-Holte
authors.forename: Laura
authors.initials: L
authors.lastname: Lien
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D005615:Freezing; D007150:Immunohistochemistry; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D010641:Phenotype; D011786:Quality Control; D051381:Rats; D014021:Tissue Preservation
chemical_list: D003470:Culture Media