title: [The significance of DNA-binding inhibitor 4 promoter methylation status in acute leukemia].
abstract: OBJECTIVE
  To investigate the relationship between promoter methylation of inhibitor of DNA binding 4 (Id4) gene and acute leukemia.
  
  
  METHODS
  We detected the status of promoter methylation of Id4 gene in peripheral blood, peripheral blood stem cell transplantation (PBSC) and bone marrow samples from health donors, bone marrow cells from leukemia patients and leukemia cell lines with MS-PCR methods.
  
  
  RESULTS
  The frequency of Id4 promoter methylation was 21/25 in acute myeloid leukemia (84%), 12/14 in acute lymphoblastic leukemia (86%), 8/8 in relapse acute leukemias and 2/2 in leukemia cell lines whereas no methylation was detected in peripheral blood, PBSC and bone marrow samples from healthy donors. In addition, Hek937 cell line was unmethylated.
  
  
  CONCLUSIONS
  Id4 promoter methylation was detected only in acute leukemia patients and leukemia cell lines and it might play a role in leukemia genesis.
authors.affiliation: Department of Hematology, General Hospital of PLA, Beijing 100853, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Quan-shun
authors.initials: QS
authors.lastname: Wang
authors.forename: Hong-hua
authors.initials: HH
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Bo
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Dou
mesh_terms: D019175:DNA Methylation; D006801:Humans; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics / Q000378:metabolism*; D020014:K562 Cells / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D020298:U937 Cells / Q000378:metabolism
chemical_list: C490128:ID4 protein, human; D051796:Inhibitor of Differentiation Proteins