title: Bioavailability of seocalcitol IV: evaluation of lymphatic transport in conscious rats.
abstract: OBJECTIVE
  To study the use of long chain triglycerides (LCT) as a lymphotropic carrier of (3)H-seocalcitol by comparing the lymphatic transport and the portal absorption of (3)H-seocalcitol when dissolved in a (1) LCT solution or a (2) reference solution without lipid containing propylene glycol (PG).
  
  
  METHODS
  A lymph cannulated conscious rat model was dosed orally with (3)H-seocalcitol dissolved in either LCT or PG. Lymph was collected continuously, and blood was sampled over 9 h. (3)H-seocalcitol in blood and lymph and triglycerides in lymph were analysed.
  
  
  RESULTS
  A statistically significantly (p < 0.05) higher recovery of the dosed (3)H-seocalcitol was found in the intestinal lymph upon administration of the LCT solution (1.3 +/- 0.6%) compared to the PG solution (0.5 +/- 0.4%). The portal absorption of (3)H-seocalcitol was significantly (p < 0.05) higher from the LCT solution (16.2 +/- 2.2%) than from the PG solution (10.8 +/- 0.8%).
  
  
  CONCLUSIONS
  The LCT solution resulted in a statistical significantly higher level of lymphatic and portal transport of (3)H-seocalcitol compared with the PG solution. However, even though LCT facilitates the formation of chylomicrons, (3)H-seocalcitol favours absorption directly to the portal blood probably due to the moderate lipophilicity of the molecule.
authors.affiliation: Pharmaceutical Formulation, LEO Pharma A/S, Industriparken 55, 2750, Ballerup, Denmark.
authors.forename: Mette
authors.initials: M
authors.lastname: Grove
authors.forename: Jeanet L
authors.initials: JL
authors.lastname: Nielsen
authors.forename: Gitte P
authors.initials: GP
authors.lastname: Pedersen
authors.forename: Anette
authors.initials: A
authors.lastname: Müllertz
mesh_terms: D000818:Animals; D001682:Biological Availability; D001692:Biological Transport; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D041981:Gastrointestinal Tract / Q000378:metabolism; D008196:Lymph / Q000378:metabolism*; D051381:Rats; D014280:Triglycerides / Q000378:metabolism
chemical_list: D014280:Triglycerides; D002117:Calcitriol; C078903:seocalcitol