Cloning and characterization of a cDNA encoding walleye (Sander vitreum) beta-2 microglobulin.
Beta(2)-microglobulin (beta(2)m) is an essential component of the MHC class I antigen presentation pathway, necessary for heavy chain folding and peptide binding. In this study a beta(2)m cDNA isolated from a walleye head kidney library was sequenced and found to be similar to other known beta(2)m sequences. The clone encodes a predicted protein sequence of 116 residues, with a mature peptide 100 amino acids in length. This represents an inconsistency with other predicted beta(2)m protein sequences as walleye contains an additional 3 residues at the N terminus of the mature protein. Southern blot analysis showed that beta(2)m is present in one copy in the walleye genome and northern blot analysis showed expression of a 1.2Kb transcript, with high expression in kidney, spleen, gills and intestine. Characterization of this gene will enable further studies of immune function in walleye, which comprises both an important fishery and an emerging aquaculture species in Canada.
Christie
Darah
D
Department of Biology, University of Waterloo, 200 University Ave West, Waterloo, Ontario, Canada.
Wei
Guang
G
Fujiki
Kazuhiro
K
Dixon
Brian
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D018628:Gene Dosage; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010471:Perches / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D016415:Sequence Alignment; D017421:Sequence Analysis; D001613:beta 2-Microglobulin / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D001613:beta 2-Microglobulin