title: Salt-induced formation of the A-state of ferricytochrome c--effect of the anion charge on protein structure.
abstract: Structural information on partially folded forms is important for a deeper understanding of the folding mechanism(s) and the factors affecting protein stabilization. The non-native compact state of equine cytochrome c stabilized by salts in an acidic environment (pH 2.0-2.2), called the A-state, is considered a suitable model for the molten globule of cytochrome c, as it possesses a native-like alpha-helix conformation but a fluctuating tertiary structure. In this article, we extend our knowledge on anion-induced protein stabilization by determining the effect of anions carrying a double negative charge; unlike monovalent anions (which are thought to exert an 'ionic atmosphere' effect on the macromolecule), divalent anions are thought to bind to the protein at specific surface sites. Our data indicate that divalent anions, in comparison to monovalent ions, have a greater tendency to stabilize the native-like M-Fe(III)-H coordinated state of the protein. The possibility that divalent anions may bind to the protein at the same sites previously identified for polyvalent anions was evaluated. To investigate this issue, the behavior of the K88E, K88E/T89K and K13N mutants was investigated. The data obtained indicate that the mutated residues, which contribute to form the binding sites of polyanions, are important for stabilization of the native conformation; the mutants investigated, in fact, all show an increased amount of the misligated H-Fe(III)-H state and, with respect to wild-type cytochrome c, appear to be less sensitive to the presence of the anion. These residues also modulate the conformation of unfolded cytochrome c, influencing its spin state and the coordination to the prosthetic group.
authors.affiliation: Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Roma 'Tor Vergata', Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Sinibaldi
authors.forename: Maria C
authors.initials: MC
authors.lastname: Piro
authors.forename: Massimo
authors.initials: M
authors.lastname: Coletta
authors.forename: Roberto
authors.initials: R
authors.lastname: Santucci
mesh_terms: D000818:Animals; D000838:Anions / Q000494:pharmacology*; D001665:Binding Sites; D002712:Chlorides / Q000494:pharmacology; D002942:Circular Dichroism; D045304:Cytochromes c / Q000737:chemistry* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D006736:Horses / Q000378:metabolism; D007700:Kinetics; D010472:Perchlorates / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D017510:Protein Folding; D013431:Sulfates / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
chemical_list: D000838:Anions; D002712:Chlorides; D010472:Perchlorates; D013431:Sulfates; D045304:Cytochromes c; C494474:perchlorate