A complete protein pattern of cellulase and hemicellulase genes in the filamentous fungus Trichoderma reesei.
The complete protein pattern of cellulase and hemicellulase genes was studied through the Genome-wide analysis in Trichoderma reesei. The genome database revealed the presence of 39 ORFs encoding related proteins, including 32 enzymes with a catalysis domain related to cellulases and hemicellulases and 7 related proteins with a cellulose-binding module (CBM). Ten of these encoded yet undescribed enzymes, including six novel beta-glucosidases or xylosidases, two putative xylanases and two undescribed mannases. To better illustrate the relation of these 39 related proteins, four groups were created and analyzed by phylogenetic analysis: group A corresponding to xylanases, group B belonging to mannases and acting to degrade mannan; group C containing all known and putative cellulose-degrading proteins that have highly conserved CBMs; and group D containing beta-glucosidase and beta-xylosidase. Group D was the largest group, in which 8 beta-glucosidases appeared to be non-secreted proteins.
Ouyang
Jia
J
College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing, PR China.
Yan
Ming
M
Kong
Dechong
D
Xu
Lin
L
D002480:Cellulase / Q000737:chemistry* / Q000235:genetics; D002482:Cellulose / Q000737:chemistry; D030541:Databases, Genetic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D016681:Genome, Fungal; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
D002482:Cellulose; D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase