[Intrathecal administration of HSV-I amplicon vector-mediated HPPE gene therapy of nocicepion in rats with formalin pain].
OBJECTIVE
To investigate the antinociceptive effect of intrathecal administration of HSV-I amplicon vector-mediated HPPE.


METHODS
Sprague Dawley rats (290+/-30) g were randomly divided into pHSVIRES-HPPE-LacZ (SHPZ) group, pHSVIRES-LacZ (SHZ) group, and saline group (NS), and 3 d, 1 week, 2 weeks, 3 weeks, 4 weeks, and 5 weeks group,which were anesthetized with 10% chlroral hydrate 300- 350 mg/kg. A microspinal catheter was inserted into the lumbar subarachnoid space. Rats were intrathecally delivered with recombinant HSV-I amplicon vector SHPZ, SHZ or NS. The HPPE expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) and radioimmune assay. Formalin 50 microL (5%) was injected into the left hindpaw, pain intensity scoring (PIS) was used to assess the antinociceptive effect.


RESULTS
After in vivo transferring,neurocyte demonstrated strong positive signals with X-gal immunohistochemical staining. RT-PCR and L-enkephalin radioimmune assay found that the neural cells transferred foreign gene (HPPE) had effective expression. Intrathecal delivery of SHPZ showed antinociceptive effects on formalin induced pain for 5 weeks compared with SHZ.


CONCLUSIONS
This amplicon virus can transfer HPPE into rat central nerve system neural cells and express efficiently, suggesting SHPZ is satisfactory treatment for gene therapy for chronic pain. Intrathecal delivery SHPZ demonstrated antinociceptive effects on formalin induced pain.
Liu
Hui-ning
HN
Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zou
Wang-yuan
WY
Yang
Yong
Y
Wang
E
E
Li
Zhun-min
ZM
Liu
Geng-xun
GX
D000818:Animals; D004745:Enkephalins / Q000235:genetics* / Q000378:metabolism; D005557:Formaldehyde; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage*; D018259:Herpesvirus 1, Human / Q000235:genetics*; D006801:Humans; D007278:Injections, Spinal; D008297:Male; D009619:Nociceptors / Q000502:physiology; D010146:Pain / Q000139:chemically induced; D059408:Pain Management; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004745:Enkephalins; D011498:Protein Precursors; C029992:proenkephalin; D005557:Formaldehyde