Nanostructured electrochemical DNA biosensors for detection of the effect of berberine on DNA from cancer cells.
Multi walled carbon nanotubes (MWNT) in dimethylformamide (DMF) or aqueous sodium dodecyl sulfate (SDS) solution, colloidal gold nanoparticles (GNP) in phosphate buffer solution (PBS), and a GNP-MWNT mixture in aqueous SDS solution have been investigated for chemical modification of a screen-printed carbon electrode used as the signal transducer of a dsDNA-based biosensor. Differential pulse voltammetry of the DNA redox marker Co[(phen)3]3+ and the guanine moiety anodic oxidation and cyclic voltammetry with K3[Fe(CN)6] as indicator revealed substantial enhancement of the response of the biosensor, particularly when MWNT in SDS solution was used. The biosensor was used in testing of berberine, an isoquinoline plant alkaloid with significant antimicrobial and anticancer activity. Berberine had a very strong, concentration-dependent, effect on the structural stability of DNA from the human cancer cells (U937 cells) whereas non-cancer cells were changed only when berberine concentrations were relatively high 75 and 50 microg mL(-1).
Ovádeková
Renáta
R
Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, 81237 Bratislava, Slovakia. renata.ovadekova@stuba.sk
Jantová
Sona
S
Letasiová
Silvia
S
Stepánek
Ivan
I
Labuda
Ján
J
D001599:Berberine / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D003035:Cobalt / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004563:Electrochemistry; D006801:Humans; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D009369:Neoplasms / Q000737:chemistry* / Q000235:genetics; D020298:U937 Cells
D001599:Berberine; D003035:Cobalt; D004247:DNA