title: Comparative proteomic profiles of meningioma subtypes.
abstract: Meningiomas are classified into three groups (benign, atypical, and anaplastic) based on morphologic characteristics. Atypical meningiomas, which are WHO grade 2 tumors, and anaplastic meningiomas, which are WHO grade 3 tumors, exhibit an increased risk of recurrence and premature death compared with benign WHO grade 1 tumors. Although atypical and anaplastic meningiomas account for <10% of all of meningiomas, it can be difficult to distinguish them from benign meningiomas by morphologic criteria alone. We used selective tissue microdissection to examine 24 human meningiomas and did two-dimensional gel electrophoresis to determine protein expression patterns. Proteins expressed differentially by meningiomas of each WHO grade were identified and sequenced. Proteomic analysis revealed protein expression patterns unique to WHO grade 1, 2, and 3 meningiomas and identified 24 proteins that distinguish each subtype. Fifteen proteins showed significant changes in expression level between benign and atypical meningiomas, whereas nine distinguished atypical from anaplastic meningiomas. Differential protein expression was confirmed by Western blotting and immunohistochemistry. We established differential proteomic profiles that characterize and distinguish meningiomas of increasing grades. The proteins and proteomic profiles enhance understanding of the pathogenesis of meningiomas and have implications for diagnosis, prognosis, and treatment.
authors.affiliation: Surgical Neurology Branch and Protein/Peptide Sequencing Facility, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, Maryland 20892, USA.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okamoto
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Alexander O
authors.initials: AO
authors.lastname: Vortmeyer
authors.forename: Howard
authors.initials: H
authors.lastname: Jaffe
authors.forename: Youn-Soo
authors.initials: YS
authors.lastname: Lee
authors.forename: Sven
authors.initials: S
authors.lastname: Gläsker
authors.forename: Tae-Sung
authors.initials: TS
authors.lastname: Sohn
authors.forename: Weifen
authors.initials: W
authors.lastname: Zeng
authors.forename: Barbara
authors.initials: B
authors.lastname: Ikejiri
authors.forename: Martin A
authors.initials: MA
authors.lastname: Proescholdt
authors.forename: Christina
authors.initials: C
authors.lastname: Mayer
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Weil
authors.forename: Edward H
authors.initials: EH
authors.lastname: Oldfield
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Zhuang
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008577:Meningeal Neoplasms / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008579:Meningioma / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D040901:Proteomics; D015854:Up-Regulation