title: Detection of herpes simplex virus type 1 DNA in bilateral human trigeminal ganglia and optic nerves by polymerase chain reaction.
abstract: Herpes simplex virus type 1 (HSV-1) DNA was extracted from human trigeminal ganglia of 121 cadavers aged between 3 months and 94 years, and its PCR amplification was performed for the RL2 HSV-1 sequence using two pairs of primers. The HSV-1 DNA was detected in 74 of 121 patients (61%); 70/74 bilaterally, 3/74 only on the left side, and 1/74 only on the right side. Although the PCR-positive rate was significantly higher in advanced age, the correlation between the PCR-positive rate and gender was unclear. Additionally, HSV-1 DNA was not detected in any of the 50 optic nerve samples.
authors.affiliation: Department of Legal Medicine, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba, Japan. hms1466@faculty.chiba-u.jp
authors.forename: Hisako
authors.initials: H
authors.lastname: Motani
authors.forename: Koichi
authors.initials: K
authors.lastname: Sakurada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikegaya
authors.forename: Tomoko
authors.initials: T
authors.lastname: Akutsu
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Hayakawa
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Sato
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yajima
authors.forename: Kaoru
authors.initials: K
authors.lastname: Sato
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Hirotaro
authors.initials: H
authors.lastname: Iwase
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D002102:Cadaver; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006561:Herpes Simplex / Q000453:epidemiology* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009900:Optic Nerve / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017678:Sex Distribution; D012668:Trigeminal Ganglion / Q000821:virology*
chemical_list: D004279:DNA, Viral