[Determination of two polyacetylenes in Herba Lobeliae Chinensis by HPLC analysis].
OBJECTIVE
To develop an HPLC method for determination of two polyacetylenes, lobetyolin and lobetyolinin, in Herba Lobeliae Chinensis.


METHODS
C18 column was used with the mobile phase consisted of acetonitrile and water. Linear gradient elution from 10% to 40% acetonitrile in 25 min was applied, at the flow rate of 1.0 mL x min(-1), the detection wavelength was at 267 nm.


RESULTS
Lower contents of lobetyolin and lobtyolinin were found in collected samples of Herba Lobeliae Chinensis. The highest amounts of lobetyolin and lobetyolinin were found to be 0.461 and 0.436 mg x g(-1) in a sample procured from Hong Kong. However, there were no lobetyolin and lobetyolinin in some of the samples.


CONCLUSIONS
A simple and effective HPLC method to analyze the two polyacetylenes in Herba Lobeliae Chinensis was established. It could be applied for the quality control of this herb.
Qiao
Chun-feng
CF
Institute of Chinese Medicine, Hong Kong Jockey Club, China.
He
Zhen-dan
ZD
Han
Quan-bin
QB
Song
Jing-zheng
JZ
Xu
Hong-xi
HX
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D029746:Lobelia / Q000737:chemistry*; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D053279:Polyynes / Q000032:analysis* / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results
C521561:lobetyolin; C521562:lobtyolinin; D053279:Polyynes