Glucose microfluidic biosensors based on immobilizing glucose oxidase in poly(dimethylsiloxane) electrophoretic microchips.
Here we reported a novel microfluidic biosensor with an on-column immobilized enzyme microreactor. The fabrication approach of this biosensor is simple and the enzyme microreactors with controlled sizes can be placed at any desired position on the microchip. Taking glucose oxidase (GOx) as an example, electroosmotic flow (EOF) as a driving force and amperometry as a detection method, the performance of biosensors were modulated by changing the length of enzyme reactor from 0.5 cm to 3 cm, and the linear ranges were changed from 0-8.0 mM to 0-30.0 mM with the detection limits from 42 microM to 6.5 microM. The enzyme reactor remained its 65% activity after 23 days storage. It also showed good anti-interference ability and was used to quantify glucose in human serum samples.
Zhang
Qing
Q
Key Lab of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
Xu
Jing-Juan
JJ
Chen
Hong-Yuan
HY
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis* / Q000378:metabolism; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D009995:Osmosis; D013997:Time Factors
D001786:Blood Glucose; D004129:Dimethylpolysiloxanes; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase