title: Application of an improved column characterisation system to evaluate the within and between batch variability.
abstract: The selection of a reversed-phase liquid chromatographic column with suitable selectivity for a particular separation is difficult if the brand name of the column is not known. A project to develop a chromatographic test procedure to characterize reversed-phase liquid chromatography C18 columns was started earlier and resulted in a fast, simple, repeatable and reproducible test procedure using four column parameters. Here, this procedure is used to evaluate the diversity of columns originating from the same batch as well as from different batches. The determination of one of the parameters, the retention factor of 2,2'-dipyridyl, was improved and a simplified test procedure is proposed.
authors.affiliation: Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Analyse, O&N 2, PB 923, Herestraat 49, B-3000 Leuven, Belgium.
authors.forename: Erik
authors.initials: E
authors.lastname: Haghedooren
authors.forename: Agnes
authors.initials: A
authors.lastname: Kerner
authors.forename: Béla
authors.initials: B
authors.lastname: Noszál
authors.forename: Jos
authors.initials: J
authors.lastname: Hoogmartens
authors.forename: Erwin
authors.initials: E
authors.lastname: Adams
mesh_terms: D015082:2,2'-Dipyridyl / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D015082:2,2'-Dipyridyl