A DNA chip-based spoligotyping method for the strain identification of Mycobacterium tuberculosis isolates.
The clinical efficacy of a spoligotyping method for discriminating Mycobacterium tuberculosis strains was evaluated. Among the strains other than Beijing or Beijing like family, 30 different spoligotypes out of 39 strains were produced, which included 4 strains not having IS6110 sequence. The oligonucleotide chip-based spoligotyping technique is useful for early screening and detection of clonal proximity of M. tuberculosis isolates.
Song
Eun Ju
EJ
Department of Laboratory Medicine, School of Medicine, Pusan National University, Seo-Gu Ami-Dong 1-10, Busan 602-739, Republic of Korea.
Jeong
Hae Jin
HJ
Lee
Sun Min
SM
Kim
Cheol Min
CM
Song
Eun Sil
ES
Park
Young Kil
YK
Bai
Gill-Han
GH
Lee
Eun Yup
EY
Chang
Chulhun L
CL
D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D014376:Tuberculosis / Q000382:microbiology*
D004269:DNA, Bacterial; D021901:DNA, Intergenic