Paracrine VEGF/VE-cadherin action on ovarian cancer permeability.
Ascites formation associated with neoplasms is most likely due to increased vascular permeability, a process in which vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) plays a pivotal role. We hypothesized that tumor-derived VEGF/VPF modulates ascites formation through a paracrine effect on both tumor and peritoneal vessels. We investigated human vascular endothelial permeability using a newly developed dual-chamber permeability assay by co-culturing human umbilical vein cells with and without ovarian cancer cell lines (OVCAR-3, Hey-A8, and OCC-1) in the presence or absence of a human VEGF monoclonal antibody and VE-cadherin function-blocking antibody. This method permits determination of mechanisms by which substances released from neoplasms and other sources of vascular endothelial cell secretagogues modulate vascular permeability and likely other pathologic states.
Hu
Limin
L
Center for Reproductive Sciences, University of California, San Francisco, 505 Parnassus Avenue, HSW 1450, San Francisco, CA 94143-0556, USA.
Ferrara
Napoleone
N
Jaffe
Robert B
RB
D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D015153:Blotting, Western; D015820:Cadherins / Q000276:immunology / Q000378:metabolism*; D002199:Capillary Permeability / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000276:immunology / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D042442:Vascular Endothelial Growth Factors / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D015820:Cadherins; D036341:Intercellular Signaling Peptides and Proteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors