Cloning and expression analysis of an IL-6 homolog in rainbow trout (Oncorhynchus mykiss).
A partial cDNA with significant similarity to IL-6 was identified in rainbow trout. Rapid amplification of cDNA ends was used to obtain the full sequence of the trout IL-6 homolog which contains 1180 nucleotides. The transcript encodes a predicted protein of 219 amino acids and eight instability motifs in the 3'UTR. While the complete sequence of the trout IL-6 is poorly conserved, the protein contains a distinct IL-6/G-CSF/MGF family consensus pattern and predicted characteristic alpha-helical tertiary structure. However, like in fugu, trout IL-6 lacks a pair of cysteine residues, which in mammals are involved in formation of a disulphide bond. The expression of the IL-6 homolog in trout mononuclear phagocytes was highly up-regulated by LPS but not poly(I:C) as demonstrated by Northern analysis. Using RT-PCR the IL-6 expression was detected in trout spleen, gill, gastrointestinal tract, ovary and brain. The highest transcript levels were detected in the ovary suggesting that IL-6 may perform specific functions within this organ.
Iliev
Dimitar B
DB
Great Lakes WATER Institute, University of Wisconsin Milwaukee, 600 E. Greenfield Avenue, Milwaukee, WI 53204, USA.
Castellana
Barbara
B
Mackenzie
Simon
S
Planas
Josep V
JV
Goetz
Frederick W
FW
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence; D015870:Gene Expression; D015850:Interleukin-6 / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D015850:Interleukin-6; D012333:RNA, Messenger