title: The accuracy of electrical method for microleakage evaluation by a three-dimensional analysis.
abstract: OBJECTIVE
  This in vitro study aimed to investigate the accuracy of an electrical method for the evaluation of microleakage by a three-dimensional analysis of dye penetration.
  
  
  METHODS
  Coronal cavities were prepared on buccal, palatal or lingual surfaces in extracted human molars. The cavities were then filled with resin composites and were subjected to 10,000 load cycles (425g). Before cavity preparation and after load cycling, physiological saline was applied and wiped off, and the change in conductance was measured across the margin of the restoration in each specimen. After dye penetration, the specimens were reduced by 100 microm in a direction parallel to the cavity floor, from the surface of the restoration to the cavity floor. The sequence of reducing the sections by 100 microm and image taking was repeated. Three-dimensional images of dye penetration were made and the proportions of the interface showing penetration were calculated.
  
  
  RESULTS
  Pearson's correlation coefficients between changes in conductance and the surface area of dye penetration, between these and the rate of dye penetration, and between these and the depth of dye penetration were 0.932, 0.920 and 0.732, respectively. The correlations were significant (p<0.05).
  
  
  CONCLUSIONS
  The results of this electrical method for microleakage evaluation showed stronger correlations with the three-dimensional amount of marginal leakage (surface area of dye penetration and rate of dye penetration) than the two-dimensional amount (depth of dye penetration).
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan. y-iwami@dent.osaka-u.ac.jp
authors.forename: Yukiteru
authors.initials: Y
authors.lastname: Iwami
authors.forename: Mikako
authors.initials: M
authors.lastname: Hayashi
authors.forename: Fumio
authors.initials: F
authors.lastname: Takeshige
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ebisu
mesh_terms: D004396:Coloring Agents; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003736:Dental Cavity Lining; D003737:Dental Cavity Preparation / Q000379:methods; D003743:Dental Enamel / Q000648:ultrastructure; D003763:Dental Leakage / Q000175:diagnosis*; D003764:Dental Materials / Q000737:chemistry; D003804:Dentin / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry; D004553:Electric Conductivity; D004563:Electrochemistry; D004568:Electrodiagnosis / Q000379:methods*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry; D008751:Methylene Blue; D019279:Resin Cements / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties
chemical_list: C112828:Clearfil AP-X; C112274:Clearfil Liner Bond 2; C084544:Clearfil Linerbond System; D004396:Coloring Agents; D003188:Composite Resins; D003764:Dental Materials; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D019279:Resin Cements; D008751:Methylene Blue