title: Atrioventricular valve development during late embryonic and postnatal stages involves condensation and extracellular matrix remodeling.
abstract: Although the signaling molecules regulating the early stages of valvular development have been well described, little is known on the late steps leading to mature fibrous leaflets. We hypothesized that atrioventricular (AV) valve development continues after birth to adjust to the postnatal maturation of the heart. By doing a systematic analysis of the AV valves of mice from embryonic day (E) 15.5 to 8 weeks old, we identified key developmental steps that map the maturation process of embryonic cushion-like leaflets into adult stress-resistant valves. Condensation of the mesenchymal cells occurred between E15.5 and E18.5 and was accompanied by increased cellular proliferation and adhesion. Cellular proliferation also contributed transiently to the concomitant elongation of the leaflets. Patterning of the extracellular matrix (ECM) proteins along the AV axis was achieved 1 week after birth, with the differentiation of two reciprocal structural regions, glycosaminoglycans and versican at the atrial side, and densely packed collagen fibers at the ventricular side. Formation and remodeling of the nodular thickenings at the closure points of the leaflets occurred between N4.5 and N11.5. In conclusion, AV valve development during late embryonic and postnatal stages includes condensation, elongation, formation of nodular thickenings, and remodeling of tension-resistant ECM proteins.
authors.affiliation: Cardiovascular Research Institute, Department of Cell Biology and Molecular Medicine, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07103, USA.
authors.forename: Boudewijn P T
authors.initials: BP
authors.lastname: Kruithof
authors.forename: Steven A
authors.initials: SA
authors.lastname: Krawitz
authors.forename: Vinciane
authors.initials: V
authors.lastname: Gaussin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D049109:Cell Proliferation; D004622:Embryo, Mammalian / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006321:Heart / Q000196:embryology / Q000254:growth & development; D006346:Heart Septum / Q000196:embryology / Q000254:growth & development; D006351:Heart Valves / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism*; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D008815:Mice, Inbred Strains
chemical_list: D016326:Extracellular Matrix Proteins