title: Evaluation of direct fluorescent antibody test for the diagnosis of bovine genital campylobacteriosis.
abstract: The direct fluorescent antibody test (DFAT) for the diagnosis of Bovine Genital Campylobacteriosis was assessed for its detection limit, observer effect, sensitivity and specificity. In addition, the specificity of the fluorescent conjugate was tested against Campylobacter sp, Arcobacter sp, Helicobacter sp, E. coli and other bacteria from the preputial flora. Ten - fold dilutions of C. fetus subsp. venerealis NCTC 10354 in PBS or preputial washings with or without centrifugation were used. All experiments were done in duplicate by three observers. Positive and negative controls were included in each assay. The detection limits of DFAT were 10(4) CFU/ ml for PBS and non - centrifuged preputial washings and 10(2) CFU/ ml for centrifuged preputial washings. There was no observer effect. The sensitivity and specificity of DFAT were 92.59% and 88.89%, respectively. The DFAT was observed to be sensitive, specific and the effect of experienced observers was minimal on test performance.
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais. Av. Antônio Carlos 6627, Caixa Postal 567, 30123-970 Belo Horizonte, MG, Brazil.
authors.forename: Josely
authors.initials: J
authors.lastname: Ferreira Figueiredo
authors.forename: Aiesca
authors.initials: A
authors.lastname: Oliveira Pellegrin
authors.forename: Cid
authors.initials: C
authors.lastname: Bastos Fóscolo
authors.forename: Raquel
authors.initials: R
authors.lastname: Paula Machado
authors.forename: Karina
authors.initials: K
authors.lastname: Leite Miranda
authors.forename: Andrey
authors.initials: A
authors.lastname: Pereira Lage
mesh_terms: D000818:Animals; D001947:Breeding / Q000592:standards; D002169:Campylobacter Infections / Q000150:complications / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002168:Campylobacter fetus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D002498:Centrifugation; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary*; D007248:Infertility, Male / Q000209:etiology / Q000662:veterinary; D008297:Male; D015588:Observer Variation; D010413:Penis / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D007507:Therapeutic Irrigation