title: Autologous bone marrow stem cell mobilization induced by granulocyte colony-stimulating factor after subacute ST-segment elevation myocardial infarction undergoing late revascularization: final results from the G-CSF-STEMI (Granulocyte Colony-Stimulating Factor ST-Segment Elevation Myocardial Infarction) trial.
abstract: OBJECTIVE
  The purpose of this investigator-driven, prospective, randomized, double-blinded, placebo-controlled phase II study was to compare the effects of granulocyte colony-stimulating factor (G-CSF) on the improvement of myocardial function in patients undergoing delayed percutaneous coronary intervention (PCI) for ST-segment elevation myocardial infarction (STEMI).
  
  
  BACKGROUND
  Experimental and early clinical studies suggest that transplantation of stem cells improves cardiac regeneration and neovascularization after acute myocardial infarction. Most investigators have utilized either a direct injection or intracoronary infusion of bone marrow-derived cells, but early cytokine-mediated mobilization of stem cells has been reported to show similar improvement in cardiac function.
  
  
  METHODS
  Forty-four patients with late revascularized subacute STEMI were treated either with G-CSF or placebo over 5 days after successful PCI. Primary end points were change of global and regional myocardial function from baseline (1 week after PCI) to 3 months after PCI assessed by magnetic resonance imaging (MRI). Secondary end points consisted of characterization of mobilized stem cell populations, assessment of safety parameters up to 12 months including 6-month angiography, as well as myocardial perfusion assessed by MRI.
  
  
  RESULTS
  Global myocardial function from baseline (1 week after PCI) to 3 months improved in both groups, but G-CSF was not superior to placebo (Delta(ejection fraction) 6.2 +/- 9.0 vs. 5.3 +/- 9.8%, p = 0.77). A slight but non-significant improvement of regional function occurred in both groups. Granulocyte colony-stimulating factor resulted in mobilization of endothelial progenitor cell populations and was well tolerated with a similar rate of target lesion revascularization from in-stent restenosis. In both groups major adverse cardiovascular events occurred in a comparable frequency. Granulocyte colony-stimulating factor resulted in significant improvement of myocardial perfusion 1 week and 1 month after PCI.
  
  
  CONCLUSIONS
  Granulocyte colony-stimulating factor treatment after PCI in subacute STEMI is feasible and relatively safe. However, patients do not benefit from G-CSF when PCI is performed late. Granulocyte colony-stimulating factor results in improved myocardial perfusion of the infarcted area, which may reflect enhanced neovascularization.
authors.affiliation: Medical Clinic I-Department of Cardiology, Ludwig Maximilians University, Klinikum Grosshadern, Munich, Germany.
authors.forename: Markus G
authors.initials: MG
authors.lastname: Engelmann
authors.forename: Hans D
authors.initials: HD
authors.lastname: Theiss
authors.forename: Christine
authors.initials: C
authors.lastname: Hennig-Theiss
authors.forename: Armin
authors.initials: A
authors.lastname: Huber
authors.forename: Bernd J
authors.initials: BJ
authors.lastname: Wintersperger
authors.forename: Anja-Eva
authors.initials: AE
authors.lastname: Werle-Ruedinger
authors.forename: Stefan O
authors.initials: SO
authors.lastname: Schoenberg
authors.forename: Gerhard
authors.initials: G
authors.lastname: Steinbeck
authors.forename: Wolfgang-M
authors.initials: WM
authors.lastname: Franz
mesh_terms: D015906:Angioplasty, Balloon, Coronary; D002318:Cardiovascular Diseases / Q000209:etiology; D003326:Coronary Circulation / Q000187:drug effects; D004311:Double-Blind Method; D004562:Electrocardiography; D016179:Granulocyte Colony-Stimulating Factor / Q000009:adverse effects / Q000627:therapeutic use*; D006321:Heart / Q000503:physiopathology; D019650:Hematopoietic Stem Cell Mobilization / Q000009:adverse effects; D006801:Humans; D008279:Magnetic Resonance Imaging; D009203:Myocardial Infarction / Q000175:diagnosis / Q000503:physiopathology / Q000628:therapy*; D013997:Time Factors
chemical_list: D016179:Granulocyte Colony-Stimulating Factor