Autoantigen arrays for multiplex analysis of antibody isotypes.
We describe here a microarray-based method for multiplexed, antigen-specific assessment of immunoglobulin (Ig) subclasses. We used 1152-feature arrays composed of 140 antigens or antigen fragments to detect isotype-specific mAb, to quantitatively monitor changes in isotype mAb concentration, and to profile antigen-specific antibody isotype production in a murine model of autoimmunity. This platform can be easily adapted to a variety of applications, and has the potential to elucidate mechanisms that govern development and evolution of antibody responses in in vivo and in vitro systems.
Graham
Kareem L
KL
Department of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Vaysberg
Maria
M
Kuo
Annie
A
Utz
Paul J
PJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D005069:Evaluation Studies as Topic; D007074:Immunoglobulin G / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000032:analysis* / Q000276:immunology; D051379:Mice; D040081:Protein Array Analysis
D000911:Antibodies, Monoclonal; D001324:Autoantigens; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes