title: [Determination of diltiazem hydrochloride injection by high-performance liquid chromatography].
abstract: OBJECTIVE
  To establish an high-performance liquid chromatography (HPLC) method for the determination of diltiazem hydrochloride injection.
  
  
  METHODS
  The assay was conducted on a NUCLEODUR C18 column with buffer solution (pH6.2)-acetonitrile-methanol (40:30:30) as the mobile phase and the detection wavelength was 240 nm.
  
  
  RESULTS
  Diltiazem hydrochloride and relevant substances could be separated under the condition. The linear concentration range of diltiazem hydrochloride was 4 - 200mg/L with the recovery of 100.22% (RSD = 0.37%).
  
  
  CONCLUSIONS
  The method may be used for the determination of diltiazem hydrochloride injection.
authors.affiliation: Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Tang
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Xin-zhong
authors.initials: XZ
authors.lastname: Li
mesh_terms: D002121:Calcium Channel Blockers / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004110:Diltiazem / Q000032:analysis*
chemical_list: D002121:Calcium Channel Blockers; D004110:Diltiazem