In vitro deoxynucleotidylation of the terminal protein of Streptomyces linear chromosomes.
Single-stranded gaps at the 3' ends of Streptomyces linear replicons are patched by DNA synthesis primed by terminal proteins (TP) during replication. We devised an in vitro system that specifically incorporated dCMP, the first nucleotide at the 5' ends, onto a threonine residue of the TP of Streptomyces coelicolor.
Yang
Chien-Chin
CC
Department of Chemistry, Chung-Yuan Christian University, Chung-li, Taiwan.
Chen
Yi-Hong
YH
Tsai
Hsiu-Hui
HH
Huang
Chih-Hung
CH
Huang
Tzu-Wen
TW
Chen
Carton W
CW
D001426:Bacterial Proteins / Q000378:metabolism*; D002876:Chromosomes, Bacterial / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism; D003843:Deoxycytidine Monophosphate / Q000378:metabolism*; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010761:Phosphorus Radioisotopes; D003843:Deoxycytidine Monophosphate