The prospects of advanced molecular methods in the detection of periodontal pathogens: hybridization in situ.
The paper summarizes the present state in the diagnostics of periodontal pathogens. Both the main advantages and drawbacks of the classic cultivation methods and those of the new DNA techniques are discussed. From the emerging methods of molecular diagnostics, the method of in situ hybridization is presented in more details. Its principle, various modifications of performance and possibilities of use are explained, including examples of its application in the detection of periodontal pathogens.
Novotná
G
G
Institut of Microbiology of the Academy of Sciences CR, Prague, Czech Republic. gnovotna@biomed.cas.cz
Hercík
K
K
Janata
J
J
Branny
P
P
Dusková
J
J
D001419:Bacteria / Q000145:classification*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D010510:Periodontal Diseases / Q000382:microbiology*