Plasma levels of t-PA and PAI-1 correlate with the formation of experimental post-surgical peritoneal adhesions.
This study has evaluated whether systemic changes of plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) parallel the adhesions development and whether they could be used as predictors of adhesion risk. This has been studied in an animal model of post-surgical peritoneal adhesion by monitoring for 10 days the plasma and tissue levels of t-PA and PAI-1. The results showed that both tissular and plasmatic levels of t-PA were decreased in concomitance with the development of peritoneal adhesions. In contrast, PAI-1 was found increased into the tissue and into the plasma samples of the rats taken at 5 and 10 days time points. Inflammatory mediators such as ICAM-1, VCAM-1, and IL-6 within the peritoneal lavage fluid also correlated with the adhesion formation process. In conclusion, post-surgical peritoneal adhesions provide alterations of local inflammatory components and local and systemic fibrinolytic components, possibly with PAI-1 quenching t-PA. This may have potential for the identification of high-risk patients.
Di Filippo
Clara
C
Section of Pharmacology L Donatelli, Department of Experimental Medicine, Second University of Naples, Naples, Italy.
Falsetto
Alessandro
A
De Pascale
Vito
V
Tufariello
Elisabetta
E
De Lucia
Domenico
D
Rossi
Francesco
F
D'Amico
Michele
M
Cennamo
Antonio
A
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D010532:Peritoneal Diseases / Q000097:blood* / Q000601:surgery; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood*; D010960:Plasminogen Activators / Q000097:blood*; D011237:Predictive Value of Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D017395:Plasminogen Activator Inhibitor 1; D010960:Plasminogen Activators