title: Antimicrobial resistance of Helicobacter pylori isolates from Iranian adults and children.
abstract: BACKGROUND
  Helicobacter pylori isolates from 84 adults and 51 children were assessed during the period of 2001 through 2004 to find out whether their susceptibilities to metronidazole, clarithromycin, amoxicillin, tetracycline, and furazolidone differ between the two groups or have changed compared with the results from our previous study done between 1997 - 2000.
  
  
  METHODS
  Biopsies from 135 dyspeptic patients were cultured on Brucella blood agar. Pure cultures of H. pylori isolates were used for antibiotic susceptibility tests. Disk diffusion method was recruited to assess the susceptibility of H. pylori isolates to different dilutions of the above mentioned antimicrobials. Results obtained from the two groups were compared and minimum inhibitory concentrations determined.
  
  
  RESULTS
  Thirty-five percent of H. pylori isolates from adults and 37% from children were resistant to metronidazole. Clarithromycin resistance rate was 2.4% and 5.9% in H. pylori isolates from adults and children, respectively. Amoxicillin resistance was 2.4% in isolates from adults and 5.9% in isolates from children. Tetracycline resistance rate was 0% in H. pylori isolates from adults and 2.0% in isolates from children. Resistance to furazolidone was not observed.
  
  
  CONCLUSIONS
  Resistance rates of H. pylori isolates from adults and children to metronidazole, clarithromycin, amoxicillin, tetracycline, and furazolidone are similar and not significantly affected by age and time.
authors.affiliation: Department of Microbiology, Faculty of Sciences, Tehran University, PO Box 14155-6455, Tehran, Iran. siavoshi@khayam.ut.ac.ir
authors.forename: Farideh
authors.initials: F
authors.lastname: Siavoshi
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Safari
authors.forename: Danna
authors.initials: D
authors.lastname: Doratotaj
authors.forename: Gholam-Reza
authors.initials: GR
authors.lastname: Khatami
authors.forename: Gholam-Hossein
authors.initials: GH
authors.lastname: Fallahi
authors.forename: Mohamad-Mehdi
authors.initials: MM
authors.lastname: Mirnaseri
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000658:Amoxicillin / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D017291:Clarithromycin / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005260:Female; D005664:Furazolidone / Q000494:pharmacology; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D007492:Iran; D008297:Male; D008795:Metronidazole / Q000494:pharmacology; D008875:Middle Aged; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D008795:Metronidazole; D005664:Furazolidone; D000658:Amoxicillin; D013752:Tetracycline; D017291:Clarithromycin