title: DC-LAMP stains pulmonary adenocarcinoma with bronchiolar Clara cell differentiation.
abstract: DC-LAMP is a molecule expressed in mature dendritic cells, but its mRNA is also found in the lung. This study compares the immunostaining spectrum of PE-10, an antisurfactant protein monoclonal antibody; thyroid transcription factor-1 (TTF-1); and DC-LAMP in normal and neoplastic lung in an attempt to characterize the cell type(s) that express DC-LAMP. Electron microscopy was used to define cell types. DC-LAMP marks pulmonary adenocarcinomas that show Clara cell characteristics by electron microscopy. In contrast, PE-10 labels tumors that have Clara cell and type II pneumocyte differentiation. DC-LAMP staining was lost in solid type adenocarcinomas but persisted in well-differentiated areas. CC-10, an antibody that marks Clara cells, was also positive in tumors that labeled for DC-LAMP. There was no prognostic difference in tumors that reacted with DC-LAMP. DC-LAMP and CC-10 reactivity was also observed in endometrial adenocarcinomas but not in other tumor types.
authors.affiliation: Department of Pathology, New York University School of Medicine, NY 10016, USA.
authors.forename: Lee-Ching
authors.initials: LC
authors.lastname: Zhu
authors.forename: Joon
authors.initials: J
authors.lastname: Yim
authors.forename: Luis
authors.initials: L
authors.lastname: Chiriboga
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Cassai
authors.forename: Gurdip S
authors.initials: GS
authors.lastname: Sidhu
authors.forename: Andre L
authors.initials: AL
authors.lastname: Moreira
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000276:immunology; D001980:Bronchi / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D018288:Carcinoma, Small Cell / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D008175:Lung Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D051907:Lysosomal Membrane Proteins / Q000032:analysis* / Q000276:immunology; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis; D011379:Prognosis; D011663:Pulmonary Surfactants / Q000032:analysis; D015996:Survival Rate; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D051907:Lysosomal Membrane Proteins; C000614412:NKX2-1 protein, human; D009687:Nuclear Proteins; D011663:Pulmonary Surfactants; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors