[Determination of notoginsenoside R1 and ginsenoside Rg1 in Rupixiao tablets by HPLC].
OBJECTIVE
To determine the content of notoginsenoside R1 and ginsenoside Rg1 in Rupixiao tablet by reverse-phase high performance liquid chromatography.


METHODS
A Kromacil C18 column was used with a mixture of acetonitrile and 0.05% phosphoric acid solution (20:80) as the mobile phase at a flow rate of 1.2 mL x min(-1) with the detection wavelength at 203 nm.


RESULTS
The measurement proved to be linear over the range of 0.941-9.41 g for notoginsenoside R1 and 1.04-10.4 g for ginsenoside Rg1. The average recovery of this method was 97.3% and 97.9% respectively.


CONCLUSIONS
The method was simple, reliable, and accurate and can be used for the quality control of this preparation.
Xu
Guo-bing
GB
China Pharmaceutical University, Nanjing.
Wang
Zheng-tao
ZT
Pan
Nie
N
Zhao
Zhe
Z
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D036145:Ginsenosides / Q000032:analysis*; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; C035054:ginsenoside Rg1; C072936:notoginsenoside R1