Effective immobilization of subunit protein in mesoporous silica modified with ethanol.
Ethoxylated FSM-type mesoporous silica (folded-sheet mesoporous material) with a pore diameter of 6.2 nm (FSM6.2) remarkably enhances rigidly of the structure in aqueous solutions. The esterified material could be used successfully as an adsorbent to accommodate subunit protein, methemoglobin (Fe(3+)). Furthermore, methemoglobin (Fe(3+)) in the pores of ethoxy-FSM is maintained a peroxidase activity similar to the native, indicating methemoglobin retains its fore subunit structure in the pores of FSM6.2.
Itoh
Tetsuji
T
National Institute of Advanced Industrial Science and Technology (AIST), Nigatake 4-2-1, Miyagino-ku, Sendai 983-8551, Japan. t-itoh@ni.aist.go.jp
Ishii
Ryo
R
Ebina
Takeo
T
Hanaoka
Takaaki
T
Ikeda
Takuji
T
Urabe
Yoko
Y
Fukushima
Yoshiaki
Y
Mizukami
Fujio
F
D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry*; D008422:Materials Testing; D008706:Methemoglobin / Q000737:chemistry*; D016062:Porosity; D011485:Protein Binding; D021122:Protein Subunits; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D021122:Protein Subunits; D014867:Water; D000431:Ethanol; D012822:Silicon Dioxide; D008706:Methemoglobin