[Effect of BST on synapse protein SYT and SYN in the hippocampus of chronic stress depression in rats].
OBJECTIVE
To explore the effects of Baisong tablets (BST) on synapse protein synatotagmin (SYT) and synaptophysin (SYN) of hippocampus in chronic stress depression in rats.


METHODS
Twenty eight male Sprague-Dawley rats were randomly allocated to 4 groups: a normal control group,a model group,a fluoxetine (FXT) group and a BST group. The normal control rats were fed in a natural environment. Rats of the model, FXT and BST groups were singly housed and given an chronic unpredicted sequence of mild stressors. The distribution and expression differences of SYT and SYN in the hippocampus of rats in different groups were investigated with in situ hybridization and immunoblotting.


RESULTS
Expressions of SYT and SYN in the hippocampus of model rats were significantly reduced, compared with that of the normal control (P<0.05); and the expressions of SYT and SYN were significantly increased in the hippocampus of the FXT and BST groups, compared with that of the model group (P<0.05).


CONCLUSIONS
The expressions of SYT and SYN protein and their mRNA decrease in the hippocampus of stress-model rats. BST can up-regulate their expression.
Tang
Feng-ying
FY
Institute of Combined of Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China. tangfengying_1976@yahoo.com.cn
Xie
Xiong-wei
XW
Hu
Sui-yu
SY
Shen
Shuang-hong
SH
D000818:Animals; D000928:Antidepressive Agents / Q000627:therapeutic use; D003863:Depression / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006624:Hippocampus / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological; D050863:Synaptotagmin I / Q000096:biosynthesis* / Q000235:genetics
D000928:Antidepressive Agents; D004365:Drugs, Chinese Herbal; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D050863:Synaptotagmin I; C504590:Sypl1 protein, rat; C494312:Syt1 protein, rat