title: Converting a breast cancer microarray signature into a high-throughput diagnostic test.
abstract: BACKGROUND
  A 70-gene tumor expression profile was established as a powerful predictor of disease outcome in young breast cancer patients. This profile, however, was generated on microarrays containing 25,000 60-mer oligonucleotides that are not designed for processing of many samples on a routine basis.
  
  
  RESULTS
  To facilitate its use in a diagnostic setting, the 70-gene prognosis profile was translated into a customized microarray (MammaPrint) containing a reduced set of 1,900 probes suitable for high throughput processing. RNA of 162 patient samples from two previous studies was subjected to hybridization to this custom array to validate the prognostic value. Classification results obtained from the original analysis were then compared to those generated using the algorithms based on the custom microarray and showed an extremely high correlation of prognosis prediction between the original data and those generated using the custom mini-array (p < 0.0001).
  
  
  CONCLUSIONS
  In this report we demonstrate for the first time that microarray technology can be used as a reliable diagnostic tool. The data clearly demonstrate the reproducibility and robustness of the small custom-made microarray. The array is therefore an excellent tool to predict outcome of disease in breast cancer patients.
authors.affiliation: Agendia BV, Slotervaart Medical Center 9D, Louwesweg 6, 1066 EC Amsterdam, The Netherlands. annuska.glas@agendia.com
authors.forename: Annuska M
authors.initials: AM
authors.lastname: Glas
authors.forename: Arno
authors.initials: A
authors.lastname: Floore
authors.forename: Leonie J M J
authors.initials: LJ
authors.lastname: Delahaye
authors.forename: Anke T
authors.initials: AT
authors.lastname: Witteveen
authors.forename: Rob C F
authors.initials: RC
authors.lastname: Pover
authors.forename: Niels
authors.initials: N
authors.lastname: Bakx
authors.forename: Jaana S T
authors.initials: JS
authors.lastname: Lahti-Domenici
authors.forename: Tako J
authors.initials: TJ
authors.lastname: Bruinsma
authors.forename: Marc O
authors.initials: MO
authors.lastname: Warmoes
authors.forename: René
authors.initials: R
authors.lastname: Bernards
authors.forename: Lodewyk F A
authors.initials: LF
authors.lastname: Wessels
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Van't Veer
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011379:Prognosis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor