[A pilot study on the impact of the drug resistance on the radioresistance in human pancreatic cancer cell lines].
OBJECTIVE
To investigate the impact of the drug resistance on the radioresistance in human pancreatic cancer cell lines.


METHODS
Three drug resistant pancreatic cancer cell sublines induced by fluorouracil (5-FU), adriamycin (ADM) and gemcitabine respectively, SW1990/FU, SW1990/ADM and SW1990/Gz, were tested for the cell cycle and radio-sensitivity with flow cytometry and clonogenic assay.


RESULTS
Compared with SW1990, the cell cycle assay indicated higher G(0)/G(1) period percentage in SW1990/FU and SW1990/Gz, but the G(2)/M period percentage decreased; SW1990/FU had the same while SW1990/Gz had lower S period percentage. SW1990/ADM almost had a similar cell cycle with SW1990. Clonogenic assay showed both SW1990/FU and SW1990/Gz had greater survival fraction (SF(2)) than SW1990, but SW1990/ADM had seemingly similar SF(2) as SW1990.


CONCLUSIONS
Drug resistant pancreatic cancer cell lines have reduced G(2)/M period percentage and increased radioresistance.
Chen
Ge
G
Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China.
Zhao
Yu-pei
YP
Jiang
Hua
H
Guo
Jun-chao
JC
Zhang
Li-yang
LY
Niu
Bei-zhan
BZ
Wu
Wei-wei
WW
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects* / Q000235:genetics; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D010190:Pancreatic Neoplasms / Q000473:pathology*; D011836:Radiation Tolerance / Q000187:drug effects*; D000093542:Gemcitabine
D000970:Antineoplastic Agents; D003841:Deoxycytidine; D004317:Doxorubicin; D005472:Fluorouracil; D000093542:Gemcitabine