Enzyme annotation with chemical tools.
Defining the activity of an enzyme in a cell-based system is preferable to in vitro biochemical techniques requiring extensive purification. Chiang et al. use small molecules, RNA interference, and metabolomics to characterize a novel enzyme upregulated in cancer cells.
Herman
Ariel G
AG
Department of Biological Sciences, Columbia University, 614 Fairchild Center, 1212 Amsterdam Avenue, MC 2406, New York, New York 10027, USA.
Stockwell
Brent R
BR
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D004798:Enzymes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D007659:Ketones / Q000737:chemistry / Q000494:pharmacology; D008246:Lysophospholipids / Q000737:chemistry / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000201:enzymology / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000378:metabolism; D034622:RNA Interference; D012680:Sensitivity and Specificity; D015854:Up-Regulation
C480312:11-hexadecenyl trifluoromethyl ketone; D004791:Enzyme Inhibitors; D004798:Enzymes; D007659:Ketones; D008246:Lysophospholipids; D010972:Platelet Activating Factor