title: Gene expression profiling reveals consistent differences between clinical samples of human leukaemias and their model cell lines.
abstract: Microarray gene expression profiles of fresh clinical samples of chronic myeloid leukaemia in chronic phase, acute promyelocytic leukaemia and acute monocytic leukaemia were compared with profiles from cell lines representing the corresponding types of leukaemia (K562, NB4, HL60). In a hierarchical clustering analysis, all clinical samples clustered separately from the cell lines, regardless of leukaemic subtype. Gene ontology analysis showed that cell lines chiefly overexpressed genes related to macromolecular metabolism, whereas in clinical samples genes related to the immune response were abundantly expressed. These findings must be taken into consideration when conclusions from cell line-based studies are extrapolated to patients.
authors.affiliation: Department of Medical Oncology, University of Berne, Berne, Switzerland.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Leupin
authors.forename: Alexandre
authors.initials: A
authors.lastname: Kuhn
authors.forename: Barbara
authors.initials: B
authors.lastname: Hügli
authors.forename: Tobias J
authors.initials: TJ
authors.lastname: Grob
authors.forename: Rolf
authors.initials: R
authors.lastname: Jaggi
authors.forename: Andreas
authors.initials: A
authors.lastname: Tobler
authors.forename: Mauro
authors.initials: M
authors.lastname: Delorenzi
authors.forename: Martin F
authors.initials: MF
authors.lastname: Fey
mesh_terms: D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000378:metabolism; D007948:Leukemia, Monocytic, Acute / Q000235:genetics / Q000378:metabolism; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000235:genetics; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism*; D015854:Up-Regulation