[Ri plasmid transformation of Glycyrrhiza uralensis and effects of some factors on growth of hairy roots].
OBJECTIVE
To study the induction of hairy roots of Glycyrrhiza uralensis and effect of some physical as well as chemical factors on the growth of hairy roots.


METHODS
Hypocotyls and cotyledons of G. uralensis were infected with Agrobacterium rhizogenes LBA9402 and A. rhizogenes R1601, respectively.


RESULTS
Hairy roots were induced by two strains of A. rhizogenes, and LBA9402 showed astronger infective ability. The frequency of transformation of hypocotyls was higher than that of cotyledons. Among the different media tested, WP medium yielded the fastest growth rate of hairy roots. Light inhibited the growth of hairy roots. The total amout of 5 flavonoids in hairy roots was 1.5 times as much as that in the callus, among them licochalcone content was 15.5 times as that of callus.


CONCLUSIONS
The in vitro culture system of the hairy roots established laid a foundation for mass production of flavonoids by hairy root culture.
Yang
Shi-hai
SH
College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China. jlyangs@yahoo.com.cn
Liu
Xiao-feng
XF
Shen
Xin
X
Zheng
Jun-hua
JH
D047188:Chalcones / Q000378:metabolism; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005419:Flavonoids / Q000378:metabolism*; D029882:Glycyrrhiza uralensis / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology; D008027:Light; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010946:Plants, Medicinal / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics / Q000502:physiology; D014170:Transformation, Genetic
D047188:Chalcones; D003470:Culture Media; D005419:Flavonoids; C070840:licochalcone A