title: A high-resolution map of Arabidopsis recombinant inbred lines by whole-genome exon array hybridization.
abstract: Recombinant populations were the basis for Mendel's first genetic experiments and continue to be key to the study of genes, heredity, and genetic variation today. Genotyping several hundred thousand loci in a single assay by hybridizing genomic DNA to oligonucleotide arrays provides a powerful technique to improve precision linkage mapping. The genotypes of two accessions of Arabidopsis were compared by using a 400,000 feature exon-specific oligonucleotide array. Around 16,000 single feature polymorphisms (SFPs) were detected in approximately 8,000 of the approximately 26,000 genes represented on the array. Allelic variation at these loci was measured in a recombinant inbred line population, which defined the location of 815 recombination breakpoints. The genetic linkage map had a total length of 422.5 cM, with 676 informative SFP markers representing intervals of approximately 0.6 cM. One hundred fifteen single gene intervals were identified. Recombination rate, SFP distribution, and segregation in this population are not uniform. Many genomic regions show a clustering of recombination events including significant hot spots. The precise haplotype structure of the recombinant population was defined with unprecedented accuracy and resolution. The resulting linkage map allows further refinement of the hundreds of quantitative trait loci identified in this well-studied population. Highly variable recombination rates along each chromosome and extensive segregation distortion were observed in the population.
authors.affiliation: Torrey Mesa Research Institute, Syngenta Research and Technology, San Diego, California, United States of America. tatjana_singer@hotmail.com
authors.forename: Tatjana
authors.initials: T
authors.lastname: Singer
authors.forename: Yiping
authors.initials: Y
authors.lastname: Fan
authors.forename: Hur-Song
authors.initials: HS
authors.lastname: Chang
authors.forename: Tong
authors.initials: T
authors.lastname: Zhu
authors.forename: Samuel P
authors.initials: SP
authors.lastname: Hazen
authors.forename: Steven P
authors.initials: SP
authors.lastname: Briggs
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D020090:Chromosome Segregation / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics; D005091:Exons / Q000235:genetics*; D018628:Gene Dosage; D018745:Genome, Plant / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020161:Physical Chromosome Mapping / Q000379:methods*; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic