Polyplex gene delivery modulated by redox potential gradients.
Polyplexes sensitive to redox potential gradients represent a promising class of vectors for delivery of nucleic acids. This review focuses on the recent advances in the development of these vectors. The biological rationale for the design of redox-sensitive polyplexes is discussed together with the basic synthetic approaches for introducing reducible disulfide bonds into the structure of the polyplexes. The biological properties of the redox-sensitive polyplexes of plasmid DNA, mRNA, antisense oligonucleotides and siRNA are reviewed with emphasis on in vitro cellular delivery, cytotoxicity and in vivo activity. Overall, redox-sensitive polyplexes represent a promising platform for further development as vectors for delivery of a wide variety of therapeutic nucleic acids.
Soundara Manickam
Devika
D
Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI 48202, USA.
Oupický
David
D
D000818:Animals; D004247:DNA / Q000008:administration & dosage*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry*; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage; D010084:Oxidation-Reduction; D010957:Plasmids / Q000008:administration & dosage; D011073:Polyamines / Q000737:chemistry; D000071228:Polyelectrolytes; D012333:RNA, Messenger / Q000008:administration & dosage; D034741:RNA, Small Interfering / Q000008:administration & dosage
D004247:DNA; D016376:Oligonucleotides, Antisense; D011073:Polyamines; D000071228:Polyelectrolytes; D012333:RNA, Messenger; D034741:RNA, Small Interfering