A centrosome-independent role for gamma-TuRC proteins in the spindle assembly checkpoint.
The spindle assembly checkpoint guards the fidelity of chromosome segregation. It requires the close cooperation of cell cycle regulatory proteins and cytoskeletal elements to sense spindle integrity. The role of the centrosome, the organizing center of the microtubule cytoskeleton, in the spindle checkpoint is unclear. We found that the molecular requirements for a functional spindle checkpoint included components of the large gamma-tubulin ring complex (gamma-TuRC). However, their localization at the centrosome and centrosome integrity were not essential for this function. Thus, the spindle checkpoint can be activated at the level of microtubule nucleation.
Müller
Hannah
H
Department of Vertebrate Genomics, Max-Planck Institute for Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, Germany.
Fogeron
Marie-Laure
ML
Lehmann
Verena
V
Lehrach
Hans
H
Lange
Bodo M H
BM
D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism; D002460:Cell Line; D018385:Centrosome / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018386:Kinetochores / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000648:ultrastructure; D008938:Mitosis; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases; D034622:RNA Interference; D008941:Spindle Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism*
C506005:BubR1 protein, Drosophila; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; C480600:Grip71 protein, Drosophila; C509152:Grip84 protein, Drosophila; D018398:Homeodomain Proteins; D008869:Microtubule-Associated Proteins; D014404:Tubulin; C098130:cnn protein, Drosophila; D011494:Protein Kinases; C090464:Bub1 spindle checkpoint protein; D017346:Protein Serine-Threonine Kinases