Crystallization and preliminary crystallographic analysis of allograft inflammatory factor 1.
Allograft inflammatory factor-1 (AIF-1) is a 17-kDa IFN-gamma inducible Ca2+-binding EF-hand protein involved in immune dysfunction and smooth muscle cell activation. AIF-1 was solubly expressed in E. coli and purified. Crystals of AIF-1 were grown at 291 K using PEG-8000 as precipitant. Diffraction by the AIF-1 crystal extends to 3.3 A resolution, and the crystal belongs to the space group P4(3) with unit cell parameters a=b=73.4, c=49.1 A.
Li
Shentao
S
The Capital University of Medical Science, Beijing, 100054, People's Republic of China. lishentao@sina.com
Liao
Xiaoping
X
Chen
Zhangliu
Z
Bartlam
Mark
M
D002135:Calcium-Binding Proteins; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008840:Microfilament Proteins; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
C112986:AIF1 protein, human; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D008840:Microfilament Proteins; D011994:Recombinant Proteins