[Molecular cytogenetic characteristics of chronic lymphocytic leukemia].
OBJECTIVE
To explore the molecular cytogenetic characteristics in patients with chronic lymphocytic leukemia (CLL).


METHODS
Interphase fluorescence in situ hybridization (FISH) was used to detect trisomy 12, deletion of 13q14 and 17p13 in 60 patients with CLL.


RESULTS
Out of the 60 patients, 41 (68.3%) had at least one kind of molecular cytogenetic aberrations. Two (3.3%) had two kinds of abnormalities. Trisomy 12 was found in 12 (20.0%) cases, 13q14 deletion in 24 (40.0%) cases and 17p13 deletion in 5 (11.7%) cases. The number of trisomy 12 cells ranged from 4.0% to 34.0%, 13q14 deletion ranged from 22.0% to 93.0% and 17p13 deletion ranged from 6.0% to 68.0%. There was no significant difference among each Binet stages.


CONCLUSIONS
FISH is a more rapid, accurate and sensitive technique in analysis of chromosome aberrations in CLL. FISH may provide accurate information of molecular cytogenetics for CLL.
Xu
Wei
W
Department of Hematology, 1st Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Li
Jian-yong
JY
Pan
Jin-lan
JL
Qiu
Hai-rong
HR
Shen
Yun-feng
YF
Xiao
Bing
B
Chen
Li-juan
LJ
Wu
Ya-fang
YF
Sheng
Rui-lan
RL
Xue
Yong-quan
YQ
D000368:Aged; D000369:Aged, 80 and over; D002872:Chromosome Deletion; D002881:Chromosomes, Human, Pair 12; D002882:Chromosomes, Human, Pair 13; D002886:Chromosomes, Human, Pair 17; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D014314:Trisomy