[Effects of cimetidine on the biological behaviors of human gastric cancer cells].
OBJECTIVE
To investigate the effects of cimetidine on the proliferation, apoptosis, adhesion, and invasion of human gastric cancer cells.


METHODS
Human gastric cancer cells of the line SGC-790 were cultured. Cimetidine of the concentrations of different concentrations was added into the culture fluid. The survival rate of the cells was calculated. Flow cytometry was used to examine the distribution of cell cycle and apoptosis of the cells. Fluorescence staining was used to observe the morphology of the apoptotic cells. The adhesion of the cells was measured by MTT method and their invasion ability was tested with Millicell chambers.


RESULTS
The proliferation of the AGC-7901 cells was inhibited by cimetidine of the concentration of 0.5 to 5 mmol/L time and dose-dependently. Cimetidine of the concentrations of 0.5 - 10 mmol/L retarded the SGC-7901 cells at the stage G(0)/G(1) and increased the number of apoptotic cells dose-dependently (P < 0.05). Cimetidine of the non-toxic concentrations 250, 125, and 62.5 micrommol/L decreased the adhesion and invasion of the SGC-7901 cells to the extracellular matrix.


CONCLUSIONS
Capable of inducing apoptosis and cell cycle arrest, cimetidine is helpful in treatment of cancers of gastric cancer.
Jiang
Cheng-gang
CG
Department of Oncology, First Hospital of China Medical University, Shenyang 110001, China.
Liu
Fu-rong
FR
Xu
Hui-mian
HM
Wu
Tao
T
Gao
Jian
J
D017209:Apoptosis / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002927:Cimetidine / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D009361:Neoplasm Invasiveness; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*
D002927:Cimetidine