title: Protective avian influenza in ovo vaccination with non-replicating human adenovirus vector.
abstract: Protective immunity against avian influenza virus was elicited in chickens by single-dose in ovo vaccination with a non-replicating human adenovirus vector encoding an H5N9 avian influenza virus hemagglutinin. Vaccinated chickens were protected against both H5N1 (89% hemagglutinin homology; 68% protection) and H5N2 (94% hemagglutinin homology; 100% protection) highly pathogenic avian influenza virus challenges. This vaccine can be mass-administered using available robotic in ovo injectors which provide a major advantage over current vaccination regimens. In addition, this class of adenovirus-vectored vaccines can be produced rapidly with improved safety since they do not contain any replication-competent adenoviruses. Furthermore, this mode of vaccination is compatible with epidemiological surveys of natural avian influenza virus infections.
authors.affiliation: Department of Pathobiology, Auburn University, Auburn, AL 36849, USA.
authors.forename: Haroldo
authors.initials: H
authors.lastname: Toro
authors.forename: De-chu C
authors.initials: DC
authors.lastname: Tang
authors.forename: David L
authors.initials: DL
authors.lastname: Suarez
authors.forename: Matt J
authors.initials: MJ
authors.lastname: Sylte
authors.forename: Jennifer
authors.initials: J
authors.lastname: Pfeiffer
authors.forename: Kent R
authors.initials: KR
authors.lastname: Van Kampen
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics* / Q000502:physiology; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D053125:Influenza A Virus, H5N2 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014779:Virus Replication
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D019444:Vaccines, DNA; C109963:hemagglutinin, avian influenza A virus