title: Extensive cross-reactive neutralizing antibody response in Indian patients with limited genetic diversity of HIV-1.
abstract: Genome sequence analysis of HIV-1 subtype C viruses from India shows monophyletic lineage and relatively limited genetic diversity. To understand its immunological implication, cross-reactivity of neutralizing antibody response was investigated. In primary screening, neutralizing antibody response to single heterologous primary HIV-1 subtype C isolate was assessed in plasma samples from 235 HIV-1 infected, anti-retroviral treatment naive individuals from Pune, India. Plasma samples that showed > or =90% neutralization and two randomly selected plasma samples that showed 50-60% neutralization were tested against a panel of primary HIV-1 subtype C isolates obtained from epidemiologically unlinked individuals from India. The neutralizing antibody response showed extensive cross-neutralization, suggesting presence of shared neutralization determinants among circulating HIV-1 subtype C viruses in India.
authors.affiliation: National AIDS Research Institute, G-73, MIDC, Bhosari, Pune 411 026, India.
authors.forename: Samir K
authors.initials: SK
authors.lastname: Lakhashe
authors.forename: Smita S
authors.initials: SS
authors.lastname: Kulkarni
authors.forename: Madhuri R
authors.initials: MR
authors.lastname: Thakar
authors.forename: Manisha V
authors.initials: MV
authors.lastname: Ghate
authors.forename: Ramesh S
authors.initials: RS
authors.lastname: Paranjape
mesh_terms: D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology*; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000378:metabolism; D015658:HIV Infections / Q000453:epidemiology / Q000276:immunology* / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D009500:Neutralization Tests
chemical_list: D000914:Antibodies, Viral; D015699:HIV Envelope Protein gp120