title: Characterization of a Vibrio alginolyticus strain, isolated from Alaskan oysters, carrying a hemolysin gene similar to the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus.
abstract: A Vibrio strain isolated from Alaskan oysters and classified by its biochemical characteristics as Vibrio alginolyticus possessed a thermostable direct hemolysin-related hemolysin (trh) gene previously reported only in Vibrio parahaemolyticus. This trh-like gene was cloned and sequenced and was 98% identical to the trh2 gene of V. parahaemolyticus. This gene seems to be functional since it was transcriptionally active in early-stationary-phase growing cells. To our knowledge, this is the first report of V. alginolyticus possessing a trh gene.
authors.affiliation: Department of Food Science, North Carolina State University, Raleigh, USA. narjol@gmail.com
authors.forename: Narjol
authors.initials: N
authors.lastname: González-Escalona
authors.forename: George M
authors.initials: GM
authors.lastname: Blackstone
authors.forename: Angelo
authors.initials: A
authors.lastname: DePaola
mesh_terms: D000413:Alaska; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010125:Ostreidae / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012758:Shellfish / Q000382:microbiology*; D044149:Vibrio alginolyticus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; C088609:thermostable direct hemolysin-related hemolysin protein, Vibrio parahaemolyticus