Decreased expression of CD25 on decidual activated T lymphocytes is not mediated by reduced CD25 messenger ribonucleic acid.
OBJECTIVE
To clarify whether the down-regulation of CD25 on decidual T cells occurred at the activated T cells and was governed through reduced CD25 messenger RNA (mRNA) production.


METHODS
Retrospective analysis and prospective study.


METHODS
University hospital and medical college.


METHODS
A total of 12 women receiving hysterectomies and 20 pregnant women having elective abortions were included.


METHODS
The amount of CD25 mRNA in isolated T cells from peripheral blood, endometrium, and decidua was analyzed with real-time polymerase chain reaction and was compared after coculture with autologous cytotrophoblast cells.


METHODS
Expression levels of CD25 and CD25 mRNA before and after coculture.


RESULTS
The percentage of activated T cells expressing CD25 is lower in decidua than in peripheral blood but the opposite in regulatory T cells. Nevertheless, the amount of CD25 mRNA in decidual T cells did not decrease, instead of approaching that in corresponding fully activated T cells. In the coculture model, we found that the cytotrophoblast cells could induce the decreased expression of CD25 on T lymphocytes. However, there was no change in the amount of CD25 mRNA in T cells after coculture.


CONCLUSIONS
This study demonstrates the effectiveness of the coculture model to study fetomaternal interactions and provides evidence that fetal cells may contribute to the control of maternal local immunity and that the decreased expression of CD25 on decidual T lymphocytes is not through the reduced CD25 mRNA level.
Chao
Kuang-Han
KH
Department of Obstetrics and Gynecology, Medical College and Hospital, National Taiwan University, Taipei, Taiwan.
Wu
Ming-Yih
MY
Yang
Jehn-Hsiahn
JH
Chen
Shee-Uan
SU
Yang
Yu-Shih
YS
Ho
Hong-Nerng
HN
D000328:Adult; D002478:Cells, Cultured; D003656:Decidua / Q000276:immunology*; D005260:Female; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology*; D011247:Pregnancy / Q000276:immunology*; D012333:RNA, Messenger / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology*
D053645:Interleukin-2 Receptor alpha Subunit; D012333:RNA, Messenger