title: [Genetic regulatory pathway of gene related breast cancer metastasis: primary study by linear differential model and k-means clustering].
abstract: OBJECTIVE
  To investigate the potential genetic regulatory pathway of gene related breast cancer metastasis.
  
  
  METHODS
  Microarray technique was used to identify the gene expression profile and to screen the differential expression genes in breast cancer with special emphasis on the metastasis factors. A gene chip was available, obtained from the surgical samples, including breast cancer primary tissues and metastasis tissues, of 30 female patients with breast cancer at different clinical stages. Then potential genetic regulatory pathway of gene related breast cancer metastasis was analyzed with a linear differential model and k-means clustering.
  
  
  RESULTS
  Twenty-seven differential expression genes were identified. It was suggested that the potential regulatory pathway of gene related to breast cancer metastasis is made up of GRP, BPAG1, and SFRP2 genes.
  
  
  CONCLUSIONS
  The metastasis of breast cancer is related to the cancerization caused by the abnormal expression of multiple genes. It is reliable to analyze the Genetic regulatory pathway of gene related breast cancer metastasis by using multiple bio-informatic measures.
authors.affiliation: Cancer Institute and Cancer Hospital, Tianjin Medical University, Tianjin 300060, China.
authors.forename: Bao-cun
authors.initials: BC
authors.lastname: Sun
authors.forename: Chun-sheng
authors.initials: CS
authors.lastname: Ni
authors.forename: Yu-mei
authors.initials: YM
authors.lastname: Feng
authors.forename: Xiao-qing
authors.initials: XQ
authors.lastname: Li
authors.forename: Shi-yi
authors.initials: SY
authors.lastname: Shen
authors.forename: Liu-huan
authors.initials: LH
authors.lastname: Dong
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Xi-shan
authors.initials: XS
authors.lastname: Hao
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D006801:Humans; D016014:Linear Models; D008207:Lymphatic Metastasis / Q000235:genetics*