title: Biochemical and electron microscopic characterization of the F1F0 ATP synthase from the hyperthermophilic eubacterium Aquifex aeolicus.
abstract: The F(1)F(0) ATP synthase has been purified from the hyperthermophilic eubacterium Aquifex aeolicus and characterized. Its subunits have been identified by MALDI-mass spectrometry through peptide mass fingerprinting and MS/MS. It contains the canonical subunits alpha, beta, gamma, delta and epsilon of F(1) and subunits a and c of F(0). Two versions of the b subunit were found, which show a low sequence homology to each other. Most likely they form a heterodimer. An electron microscopic single particle analysis revealed clear structural details, including two stalks connecting F(1) and F(0). In several orientations the central stalk appears to be tilted and/or kinked. It is unclear whether there is a direct connection between the peripheral stalk and the delta subunit.
authors.affiliation: Max-Planck-Institut für Biophysik, Max-von-Laue-Str. 3, D-60438 Frankfurt am Main, Germany.
authors.forename: Guohong
authors.initials: G
authors.lastname: Peng
authors.forename: Mihnea
authors.initials: M
authors.lastname: Bostina
authors.forename: Michael
authors.initials: M
authors.lastname: Radermacher
authors.forename: Isam
authors.initials: I
authors.lastname: Rais
authors.forename: Michael
authors.initials: M
authors.lastname: Karas
authors.forename: Hartmut
authors.initials: H
authors.lastname: Michel
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D025243:Bacterial Proton-Translocating ATPases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D017433:Protein Structure, Secondary; D021122:Protein Subunits; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D021122:Protein Subunits; D025243:Bacterial Proton-Translocating ATPases