Characterization of the porcine FSCN3 gene: cDNA cloning, genomic structure, mapping and polymorphisms.
Fascin 3 (FSCN3)is a testis-specific actin-bundling protein involved in spermatid development. Here we describe the molecular characterisation of the porcine FSCN3 gene. The 1,800-bp cDNA sequence contains a 1,497-bp open reading frame encoding a protein of 498 amino acids with a calculated molecular mass of 56.2 kDa and an isoelectric point of 6.82. The porcine FSCN3 protein shares high identity with other mammalian FSCN3. The FSCN3 gene contains seven exons, spans approximately 9 kb, and maps to pig chromosome 18. We also identified 24 DNA polymorphisms.
Kollers
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Genus plc, Genus Cambridge Research Laboratory, University of Cambridge, Department of Pathology, Cambridge, UK.
Day
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Rocha
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping / Q000662:veterinary; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D020224:Expressed Sequence Tags; D005796:Genes; D006801:Humans; D007526:Isoelectric Point; D008297:Male; D008322:Mammals / Q000235:genetics; D051379:Mice; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D021101:Radiation Hybrid Mapping; D051381:Rats; D016415:Sequence Alignment; D017385:Sequence Homology; D013045:Species Specificity; D034421:Sus scrofa / Q000235:genetics*
D018076:DNA, Complementary; D008840:Microfilament Proteins