title: [Effects of cimetidine on the biological behaviors of human gastric cancer cells].
abstract: OBJECTIVE
  To investigate the effects of cimetidine on the proliferation, apoptosis, adhesion, and invasion of human gastric cancer cells.
  
  
  METHODS
  Human gastric cancer cells of the line SGC-790 were cultured. Cimetidine of the concentrations of different concentrations was added into the culture fluid. The survival rate of the cells was calculated. Flow cytometry was used to examine the distribution of cell cycle and apoptosis of the cells. Fluorescence staining was used to observe the morphology of the apoptotic cells. The adhesion of the cells was measured by MTT method and their invasion ability was tested with Millicell chambers.
  
  
  RESULTS
  The proliferation of the AGC-7901 cells was inhibited by cimetidine of the concentration of 0.5 to 5 mmol/L time and dose-dependently. Cimetidine of the concentrations of 0.5 - 10 mmol/L retarded the SGC-7901 cells at the stage G(0)/G(1) and increased the number of apoptotic cells dose-dependently (P < 0.05). Cimetidine of the non-toxic concentrations 250, 125, and 62.5 micrommol/L decreased the adhesion and invasion of the SGC-7901 cells to the extracellular matrix.
  
  
  CONCLUSIONS
  Capable of inducing apoptosis and cell cycle arrest, cimetidine is helpful in treatment of cancers of gastric cancer.
authors.affiliation: Department of Oncology, First Hospital of China Medical University, Shenyang 110001, China.
authors.forename: Cheng-gang
authors.initials: CG
authors.lastname: Jiang
authors.forename: Fu-rong
authors.initials: FR
authors.lastname: Liu
authors.forename: Hui-mian
authors.initials: HM
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002927:Cimetidine / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D009361:Neoplasm Invasiveness; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D002927:Cimetidine