title: Evidence for pestivirus infection in free-living Japanese serows, Capricornis crispus.
abstract: Sixteen serum samples collected from free-living Japanese serows, Capricornis crispus, between 2001 and 2004 in Morioka and its vicinity were examined for the presence of pestivirus by reverse transcription-nested PCR procedure. Three out of the 16 samples produced a visible band in electrophoresed agarose gels. The nucleotide sequences of the three PCR products were found to be identical. The pestivirus found in the serow was identified as Bovine viral diarrhea virus 1 (BVDV-1) based on nucleotide sequence analyses by phylogeny as well as palindromic nucleotide substitutions at the 5' untranslated regions. Our data first indicated that BVDV-1 infection occurred continuously among the free-living serow populations though the role of BVDV-1 in wild ungulates is currently unknown.
authors.affiliation: Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan. harasawa-tky@umin.ac.jp
authors.forename: Ryô
authors.initials: R
authors.lastname: Harasawa
authors.forename: Fumio
authors.initials: F
authors.lastname: Aoyama
authors.forename: Masanobu
authors.initials: M
authors.lastname: Goryo
authors.forename: Kosuke
authors.initials: K
authors.lastname: Okada
authors.forename: Takashi
authors.initials: T
authors.lastname: Nishimura
authors.forename: Kazuei
authors.initials: K
authors.lastname: Matsubara
authors.forename: Tsunenori
authors.initials: T
authors.lastname: Tsujimoto
authors.forename: Massimo
authors.initials: M
authors.lastname: Giangaspero
mesh_terms: D000818:Animals; D000866:Antelopes / Q000821:virology*; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D007564:Japan; D018182:Pestivirus Infections / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction