Genetic diversity studies of Kherigarh cattle based on microsatellite markers.
We report a genetic diversity study of Kherigarh cattle, a utility draught-purpose breed of India, currently declining at a startling rate, by use of microsatellite markers recommended by the Food and Agriculture Organization. Microsatellite genotypes were derived, and allelic and genotypic frequencies, heterozygosities and gene diversity were estimated. A total of 131 alleles were distinguished by the 21 microsatellite markers used. All the microsatellites were highly polymorphic, with mean (+/- s.e.) allelic number of 6.24 +/- 1.7, ranging 4-10 per locus. The observed heterozygosity in the population ranged between 0.261 and 0.809, with mean (+/- s.e.) of 0.574 +/- 0.131, indicating considerable genetic variation in this population. Genetic bottleneck hypotheses were also explored. Our data suggest that the Kherigarh breed has not experienced a genetic bottleneck in the recent past.
Pandey
A K
AK
Core Lab, National Bureau of Animal Genetic Resources, Karnal 132 001, India. ashwini@nbagr.ernet.in
Sharma
Rekha
R
Singh
Yatender
Y
Prakash
B B
BB
Ahlawat
S P S
SP
D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics*; D014644:Genetic Variation; D005843:Geography; D018895:Microsatellite Repeats