High-performance liquid chromatography-electrospray ionization mass spectrometry determination of sodium ferulate in human plasma.
A selective and sensitive high-performance liquid chromatography-electrospray ionization mass spectrometry method has been developed for the determination of sodium ferulate in human plasma. The sample preparation was a liquid-liquid extraction and chromatographic separation was achieved with an Agilent ZORBAX SB-C(18) (3.5 microm, 100 mm x 3.0 mm) column, using a mobile phase of methanol-0.05% acetic acid 40:60 (v/v). Standard curves were linear (r(2)=0.9982) over the concentration range of 0.007-4.63 nM/ml and had acceptable accuracy and precision. The within- and between-batch precisions were within 12% relative standard deviation. The lower limit of quantification (LLOQ) was 0.007 nM/ml. The validated HPLC-ESI-MS method has been used successfully to study sodium ferulate pharmacokinetics, bioavailability and bioequivalence in 20 healthy volunteers.
Yang
Cheng
C
Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, China.
Tian
Yuan
Y
Zhang
Zunjian
Z
Xu
Fengguo
F
Chen
Yun
Y
D000328:Adult; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D018592:Cross-Over Studies; D004338:Drug Combinations; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
D003373:Coumaric Acids; D004338:Drug Combinations; D007202:Indicators and Reagents; C004999:ferulic acid