[Regulation of 17beta-E2 on the membrane estrogen receptor alpha].
OBJECTIVE
To investigate the regulation of 17beta-estradiol on the membrane estrogen receptor alpha.


METHODS
The Ishikawa cells were treated with 10(-6) mol/L 17beta-estradiol for 0, 3, 10, 30 minutes and 2, 12, 48 hours, respectively. Another group of Ishikawa cells was treated with 10(-12) mol/L, 10(-10) mol/L, 10(-8) mol/L and 10(-6) mol/L 17beta-estradiol for 30 minutes, respectively. The cells were marked with anti-ERalpha and measured with FACS.


RESULTS
The fluorescence intensity of mERalpha increased gradually with the application of estradiol. It reached the peak at the end of 30 minutes. Then it began to decrease gradually. When the cells were treated at low concentration of estradiol the fluorescence intensity of mERalpha was high, especially at 10(-10) mol/L estradiol. Then with the rise of the concentration, the fluorescence intensity decreased.


CONCLUSIONS
The quantity of mERalpha reached the peak at the end of 30 minutes when treated with estradiol. Then it decreased gradually. When treated with estradiol at different concentrations, its quantity could reach the peak at physiological concentration.
Wang
Zhi-qi
ZQ
Department of Gynecology, Peking University People's Hospital, Beijing 100044, China.
Wang
Jian-liu
JL
Wei
Li-hui
LH
Zhao
Dan
D
Gao
Min
M
Guo
Rui-xia
RX
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000473:pathology; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000096:biosynthesis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D013997:Time Factors
D047628:Estrogen Receptor alpha; D004958:Estradiol