Distinct effects of different temperatures on diapause termination, yolk morphology and MAPK phosphorylation in the silkworm, Bombyx mori.
Previously, we showed that chilling of diapausing Bombyx eggs activated ERK/MAPK in yolk cells coincidentally with acquisition of developmental competence, and that ERK regulates diapause termination via activating transcription of key enzyme genes for ecdysteroid and sorbitol metabolism. To further elucidate the molecular mechanisms of temperature signaling and diapause termination, effects of different temperatures on diapause termination, yolk morphology, and ERK and p38 MAPK phosphorylation were analyzed. We confirmed the coincidence of diapause termination and changes of phosphorylated levels of ERK and p38 MAPK at various temperatures. Our study also shows that different temperatures have distinct effects on diapause termination and yolk morphology, however, these two effects did not always coincide. Our analyses suggest the possibility that the effects on diapause termination and yolk morphology are independently regulated by ERK. We observed that a temperature shift alters diapause intensity reversibly, and coincidental changes of phosphorylated ERK levels suggest that ERK may have a role in regulating the diapause intensity. The present study suggests that ERK and p38 MAPK have roles for transmitting the temperature signals that are important for diapause termination.
Fujiwara
Yoshihiro
Y
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386-8567, Japan. fujiwara.6@osu.edu
Shiomi
Kunihiro
K
D000818:Animals; D012831:Bombyx / Q000196:embryology* / Q000201:enzymology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000032:analysis / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D010063:Ovum / Q000166:cytology / Q000201:enzymology / Q000254:growth & development; D010766:Phosphorylation; D013696:Temperature; D013997:Time Factors; D048051:p38 Mitogen-Activated Protein Kinases / Q000032:analysis / Q000378:metabolism*
D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases