Quantification of mixed chimerism by real time PCR on whole blood-impregnated FTA cards.
This study has investigated quantification of chimerism in sex-mismatched transplantations by quantitative real time PCR (RQ-PCR) using FTA paper for blood sampling. First, we demonstrate that the quantification of DNA from EDTA-blood which has been deposit on FTA card is accurate and reproducible. Secondly, we show that fraction of recipient cells detected by RQ-PCR was concordant between the FTA and salting-out method, reference DNA extraction method. Furthermore, the sensitivity of detection of recipient cells is relatively similar with the two methods. Our results show that this innovative method can be used for MC assessment by RQ-PCR.
Pezzoli
N
N
Laboratoire de Biochimie et Biologie Moléculaire, AP-HM, ERT-MEIDIA, Institut fédératif Jean Roche, Faculté de Médecine Nord, Université de la Méditerranée, Marseille, France.
Silvy
M
M
Woronko
A
A
Le Treut
T
T
Lévy-Mozziconacci
A
A
Reviron
D
D
Gabert
J
J
Picard
C
C
D046528:Chimerism; D004247:DNA / Q000097:blood*; D005260:Female; D018628:Gene Dosage; D005914:Globins / Q000235:genetics*; D006400:Hematocrit; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D010209:Paper; D010976:Platelet Count; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods
D011933:Reagent Kits, Diagnostic; D005914:Globins; D004247:DNA