[HPLC determination of acteoside in Radix Rehmanniae].
OBJECTIVE
To develop an HPLC method for the determination of acteoside in Radix Rehmanniae.


METHODS
The chromatographic conditions were as follows: Polaris C18(4.6 mm x 250 mm, 5 microm) column, a mobile phase in gradient mode composed of acetonitrile 0.1% acetic acid solution, a flow rate of 1.0 mL x min(-1), and 334 nm as the detection wavelength.


RESULTS
Acteoside showed good linear relationship at the range of 10-500 microg x mL(-1) (r = 0.9990). The average recovery was 100.1%, RS D 3.7%.


CONCLUSIONS
The proposed method promised to be applicable for the quality control of Radix Rehmanniae.
Li
Hui
H
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Chou
Gui-xin
GX
Wang
Zheng-tao
ZT
Hu
Zhi-bi
ZB
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D032267:Rehmannia / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D059808:Polyphenols
D005960:Glucosides; D010636:Phenols; C058956:acteoside; D059808:Polyphenols