Five years' experience of preimplantation genetic diagnosis in the Parisian Center: outcome of the first 441 started cycles.
OBJECTIVE
To investigate the evolution of techniques and strategies and to evaluate the results of preimplantation genetic diagnosis (PGD) from January 2000 to December 2004 in chromosomal, monogenic and mitochondrial DNA disorders treated at our institution.


METHODS
Retrospective study.


METHODS
Single French Parisian PGD center.


METHODS
Patients at risk of transmitting a serious genetic disorder to their offspring.


METHODS
171 couples enrolled in the program undergoing stimulated and frozen embryo replacement cycles with PGD.


METHODS
Results of the 441 first PGD cycles performed for various genetic conditions.


RESULTS
During 5 years, 416 stimulation and 25 frozen embryo replacement cycles were started, among which 52 clinical and 47 ongoing pregnancies occurred. In stimulation cycles, the overall ongoing pregnancy rate was 24% per embryo transfer, 11% per started cycle, and 27% per couple. The implantation rate was 16%.


CONCLUSIONS
These encouraging results demonstrate that PGD might be considered as a valid alternative to prenatal diagnosis. Nevertheless, couples referred for PGD must be selected and counseled appropriately, considering the complexity of the treatment and the relatively low take-home baby rate.
Feyereisen
Estelle
E
Service de Gynécologie-Obstétrique et de Médecine de la Reproduction, Hôpital Antoine Béclère, Clamart, France.
Steffann
Julie
J
Romana
Serge
S
Lelorc'h
Marc
M
Ray
Pierre
P
Kerbrat
Violaine
V
Tachdjian
Gérard
G
Frydman
René
R
Frydman
Nelly
N
D000328:Adult; D005260:Female; D005602:France / Q000453:epidemiology; D040181:Genetic Diseases, X-Linked / Q000175:diagnosis* / Q000453:epidemiology* / Q000235:genetics / Q000517:prevention & control; D020022:Genetic Predisposition to Disease / Q000453:epidemiology* / Q000235:genetics; D006801:Humans; D008137:Longitudinal Studies; D008875:Middle Aged; D017063:Outcome Assessment, Health Care / Q000379:methods* / Q000706:statistics & numerical data; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000453:epidemiology*; D019836:Preimplantation Diagnosis / Q000706:statistics & numerical data*; D011379:Prognosis; D015203:Reproducibility of Results; D012189:Retrospective Studies; D018570:Risk Assessment / Q000379:methods*; D012307:Risk Factors; D012680:Sensitivity and Specificity; D016896:Treatment Outcome