title: [A comparison study on clinical chromatographic fingerprintings of mycolic acids analysis of 18 standard strains of Mycobacteria in China].
abstract: OBJECTIVE
  A clinical chromatographic fingerprinting of 18 standard strains of Mycobacteria commercialized in China by high-performance liquid chromatography approach was developed and improved in this laboratory. A comparison of clinical chromatographic fingerprintings of mycolic acids was carried out.
  
  
  METHODS
  Mycolic acids were extracted from whole cultured cells of Mycobacteria by biochemical methods, and then samples was prepared for analysis after the mycolic acids were saponified, acidified and derived. The samples were detected by high-performance liquid chromatography under optimum conditions.
  
  
  RESULTS
  Using above techniques a clinical chromatographic fingerprinting of Mycolic acids analysis was successfully established. The repeatability of the mycolic acids fingerprinting was evaluated. Coefficient of variation (CV) of chromatographic fingerprinting was between 0.20% - 0.83%.
  
  
  CONCLUSIONS
  The first clinical chromatographic fingerprinting of 18 standard strains of Mycobacteria in China was established in this laboratory. Mycobacteria can be accurately identified according to chromatographic fingerprinting in a short time. Moreover, there was significant difference between BCG vaccine and Mycobacterium bovis when their peak was compared, which may lead to research of new tools for identifying insidious infection of tuberculosis and for the study of the mechanism of drug tolerance.
authors.affiliation: Orthopaedic Institute of Tianjin Hospital, Tianjin 300211, China.
authors.forename: Feng-yi
authors.initials: FY
authors.lastname: Zhao
authors.forename: Ju-cun
authors.initials: JC
authors.lastname: Wang
authors.forename: Xie-xiu
authors.initials: XX
authors.lastname: Wang
authors.forename: De-fu
authors.initials: DF
authors.lastname: Zhao
authors.forename: Ren-xiao
authors.initials: RX
authors.lastname: Bai
authors.forename: Su-min
authors.initials: SM
authors.lastname: Wang
authors.forename: Xue-qian
authors.initials: XQ
authors.lastname: Wang
authors.forename: Guo-sheng
authors.initials: GS
authors.lastname: Xing
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification; D009171:Mycolic Acids / Q000032:analysis*; D010449:Peptide Mapping
chemical_list: D009171:Mycolic Acids