Spatial codes in dendritic BC1 RNA.
BC1 RNA is a dendritic untranslated RNA that has been implicated in local translational control mechanisms in neurons. Prerequisite for a functional role of the RNA in synaptodendritic domains is its targeted delivery along the dendritic extent. We report here that the targeting-competent 5' BC1 domain carries two dendritic targeting codes. One code, specifying somatic export, is located in the medial-basal region of the 5' BC1 stem-loop structure. It is defined by an export-determinant stem-bulge motif. The second code, specifying long-range dendritic delivery, is located in the apical part of the 5' stem-loop domain. This element features a GA kink-turn (KT) motif that is indispensable for distal targeting. It specifically interacts with heterogeneous nuclear ribonucleoprotein A2, a trans-acting targeting factor that has previously been implicated in the transport of MBP mRNA in oligodendrocytes and neurons. Our work suggests that a BC1 KT motif encodes distal targeting via the A2 pathway and that architectural RNA elements, such as KT motifs, may function as spatial codes in neural cells.
Muslimov
Ilham A
IA
Department of Physiology and Pharmacology, The Robert F. Furchgott Center for Neural and Behavioral Science, State University of New York Health Science Center at Brooklyn, Brooklyn, NY 11203, USA.
Iacoangeli
Anna
A
Brosius
Jürgen
J
Tiedge
Henri
H
D020121:5' Untranslated Regions / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism*; D029721:Drosophila Proteins; D004622:Embryo, Mammalian; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics; D008845:Microinjections; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012313:RNA; D034443:RNA Transport; D020733:RNA, Small Cytoplasmic / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017783:Superior Cervical Ganglion; D015534:Trans-Activators / Q000235:genetics
D020121:5' Untranslated Regions; C117734:BC1 RNA; D029721:Drosophila Proteins; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D018398:Homeodomain Proteins; D020733:RNA, Small Cytoplasmic; C039919:RNA, recombinant; D015534:Trans-Activators; C056478:bcd protein, Drosophila; C439768:hnRNP A2; D012313:RNA