Quantification of magnetic nanoparticles by magnetorelaxometry and comparison to histology after magnetic drug targeting.
Magnetic nanoparticles can be used in medicine in vivo as contrast agents and as a drug carrier system for chemotherapeutics. Thus local cancer therapy is performed with Magnetic Drug Targeting (MDT) and allows a specific delivery of therapeutic agents to desired targets, i.e., tumors, by using a chemotherapeutic substance bound to magnetic nanoparticles and focused with an external magnetic field to the tumor after intraarterial application. Important for this therapeutic principle is the distribution of the particles in the whole organism and especially in the tumor. Therefore we used magnetorelaxometry to quantify ferrofluids delivered after MDT. Tissue samples of some mm3 volume of a VX2 squamous cell carcinoma were measured by magnetic relaxation and the amount of iron was determined using the original ferrofluid suspension as a reference. From this the distribution of the magnetic particles within the slice of tumor was reconstructed. Histological cross-sections of the respective tumor offer the opportunity to map quantitatively the particle distribution and the vascularisation in the targeted tumor on a microscopic scale. Our data show that the integral method magnetorelaxometry and microscopic histological methods can complete each other efficiently.
Wiekhorst
F
F
Department of Otorhinolaryngology, Head and Neck Surgery, University Erlangen-Nürnberg, Germany.
Seliger
C
C
Jurgons
R
R
Steinhoff
U
U
Eberbeck
D
D
Trahms
L
L
Alexiou
C
C
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D008280:Magnetics / Q000627:therapeutic use*; D049329:Nanostructures / Q000032:analysis* / Q000737:chemistry / Q000648:ultrastructure*; D010316:Particle Size; D011817:Rabbits