Fetal cells participate over time in the response to specific types of murine maternal hepatic injury.
BACKGROUND
In humans, fetal microchimeric cells transferred to maternal tissues during pregnancy can adopt a hepatocyte phenotype. Our objective was to determine whether fetal cells participate in the response to specific murine post-partum hepatic injuries.


METHODS
Wild-type female mice were bred to males transgenic for the enhanced green fluorescent protein (GFP) (n = 42). Following delivery, we created models of chemical or surgical injury with carbon tetrachloride (CCl(4)) injection or by performing partial hepatectomy. Liver injury was assessed histologically. Fetal cells in maternal liver were detected and measured by real-time PCR amplification of the gfp transgene and by immunofluorescence using anti-GFP antibodies.


RESULTS
PCR results showed that in chemical but not surgical injury, fetal GFP+ cells were detectable in maternal liver and spleen and that fetal cell presence was significantly increased over time following injury (4 versus 8 weeks, P = 0.006 for liver and P = 0.0006 for spleen). In some animals, following chemical injury, GFP+ cells were detected by immunofluorescence.


CONCLUSIONS
The results of this preliminary study suggest that specific types of injury may elicit different fetal cell responses in maternal organs. There is a significant effect of time on fetal cell presence in liver and spleen. Furthermore, real-time PCR amplification is more sensitive than immunofluorescence for the detection of microchimeric fetal cells.
Khosrotehrani
K
K
Division of Genetics, Department of Pediatrics, Tufts-New England Medical Center, Boston, MA 02111, USA.
Reyes
R R
RR
Johnson
K L
KL
Freeman
R B
RB
Salomon
R N
RN
Peter
I
I
Stroh
H
H
Guégan
S
S
Bianchi
D W
DW
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000503:physiopathology; D056486:Chemical and Drug Induced Liver Injury; D046528:Chimerism; D005260:Female; D005333:Fetus / Q000166:cytology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006498:Hepatectomy; D008099:Liver / Q000737:chemistry; D008107:Liver Diseases / Q000503:physiopathology*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D008431:Maternal-Fetal Exchange / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D019076:Transgenes / Q000235:genetics
D049452:Green Fluorescent Proteins