Effects of the bacteriocin PsVP-10 produced by Pseudomonas sp. on sensitive bacterial strains.
The bacteriocin PsVP-10 is a 2.6 Kda peptide which was isolated and purified from Pseudomonas sp. This bacteriocin possesses lethal activity over Enterococcus faecalis, Salmonella typhimurium and Shigella flexneri. The experimental assays showed that the bacteriocin is able to be adsorbed by all cells of these bacterial species and also by their isolated cell walls. It was observed that the resistant mutants and their respective cell walls are unable to adsorb the bacteriocin. Assays performed with spheroplasts obtained from sensitive bacterial species and their resistant mutants show a rapid lethal effect of the bacteriocin PsVP-10. This results indicated furthermore, it is also shown that the optimal pH and temperature for the adsorption were 7.2 and 37 degrees C, respectively. The study carried out with organic solvents like methanol, ethanol, isopropanol and the detergents sodium dodecyl sulfate and triton X-100 showed a moderate inhibition of the bacteriocin lethal action for the Gram negative cells. The enzymes lysozime, protease XIV and trypsine type III-S did not present any effect over the adsorption capacity of the bacteriocin with any of the bacterial species studied.
Padilla
Carlos
C
Laboratory of Research in Microbiology and Institute of Biological Sciences and Biotechnology, Universidad de Talca, Chile. cpadilla@utalca.cl
Lobos
Olga
O
Brevis
Pedro
P
Abaca
Paulina
P
Hubert
Elizabeth
E
D000327:Adsorption; D001430:Bacteriocins / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects; D003967:Diarrhea / Q000382:microbiology; D004351:Drug Resistance; D004405:Dysentery, Bacillary / Q000382:microbiology; D013293:Enterococcus faecalis / Q000187:drug effects; D006020:Glycopeptides / Q000494:pharmacology*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D011549:Pseudomonas / Q000378:metabolism*; D012480:Salmonella Infections / Q000382:microbiology; D012486:Salmonella typhimurium / Q000187:drug effects; D012763:Shigella flexneri / Q000187:drug effects; D013104:Spheroplasts / Q000187:drug effects; D013696:Temperature; D014552:Urinary Tract Infections / Q000382:microbiology
D001430:Bacteriocins; D006020:Glycopeptides; C483835:PsVP-10 peptide, Pseudomonas