title: Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability.
authors.affiliation: Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim/Ruhr, Germany. reetz@mpi-muelheim.mpg.de
authors.forename: Manfred T
authors.initials: MT
authors.lastname: Reetz
authors.forename: José D
authors.initials: JD
authors.lastname: Carballeira
authors.forename: Andreas
authors.initials: A
authors.lastname: Vogel
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D018360:Crystallography, X-Ray; D019020:Directed Molecular Evolution / Q000379:methods*; D004795:Enzyme Stability / Q000235:genetics / Q000502:physiology*; D007700:Kinetics; D008958:Models, Molecular; D016296:Mutagenesis; D011489:Protein Denaturation; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D013379:Substrate Specificity; D013696:Temperature; D013816:Thermodynamics
chemical_list: D001426:Bacterial Proteins; C079999:LipA protein, Bacteria; D011506:Proteins