title: Folate production by bifidobacteria as a potential probiotic property.
abstract: The ability of 76 Bifidobacterium strains to produce folate was investigated. In order to evaluate folic acid productivity, bifidobacteria were cultivated in the folate-free semisynthetic medium SM7. Most of the tested strains needed folate for growth. The production and the extent of vitamin accumulation were not a function of species but were distinctive features of individual strains. Six strains among the 17 that grew without folate produced significantly higher concentrations of vitamin (between 41 and 82 ng ml(-1)). The effects of exogenous folate and p-aminobenzoic acid (PABA) concentrations on folate production were evaluated. In contrast to most of the other strains, the folate yield of B. adolescentis MB 239 was not negatively affected by either PABA or exogenous folic acid. Folate production by B. adolescentis MB 239 was studied in the pH range of the colonic environment, and a comparison of folate production on raffinose, lactose, and fructo-oligosaccharides, which belong to three important groups of fermentable intestinal carbon sources, was established. Differences in folate biosynthesis by B. adolescentis MB 239 were not observed as a function either of the pH or of the carbon source. Fecal culture experiments demonstrated that the addition of B. adolescentis MB 239 may increase the folate concentration in the colonic environment.
authors.affiliation: Department of Pharmaceutical Sciences, University of Bologna, Bologna, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Pompei
authors.forename: Lisa
authors.initials: L
authors.lastname: Cordisco
authors.forename: Alberto
authors.initials: A
authors.lastname: Amaretti
authors.forename: Simona
authors.initials: S
authors.lastname: Zanoni
authors.forename: Diego
authors.initials: D
authors.lastname: Matteuzzi
authors.forename: Maddalena
authors.initials: M
authors.lastname: Rossi
mesh_terms: D001431:Bacteriological Techniques; D001644:Bifidobacterium / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D003106:Colon / Q000166:cytology / Q000378:metabolism; D003470:Culture Media; D005243:Feces / Q000382:microbiology; D005260:Female; D005492:Folic Acid / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D019936:Probiotics; D013045:Species Specificity
chemical_list: D003470:Culture Media; D005492:Folic Acid