title: Bone marrow transplantation from a pediatric donor with a high frequency of cytomegalovirus-specific T-cells.
abstract: A recent study reported that quantitation of cytomegalovirus (CMV)-specific CD8+ T lymphocytes in the graft and monitoring of these T cells might identify hematopoietic stem cell transplantation-recipients at the risk for progressive CMV infection. A 6-year-old girl underwent bone marrow transplantation from an HLA-identical sibling with a very high frequency of CMV specific tetramer-positive CD8+ T-cells. CMV-specific T-cell immunity was prospectively evaluated using a peptide (HLA-A2, NLVPMVATV). Tetramer assay showed that the frequency of CMV-specific CD8+ T cells of the donor in the peripheral blood was 5.3%, higher than average amongst young children. The frequency of CMV-specific CD8+ T cells of the donor in the graft was 3.7% of CD8+ T-cells. Before transplantation, the frequency of CMV specific CD8+ T cells of the recipient was 0.1% in the peripheral blood. Surprisingly, the frequency of CMV specific CD8+ T cells increased up to 30% of CD8+ T-cells at day 27 after transplantation. IFN-gamma enzyme-linked immunospot assay showed the recipient-T cells had strong responses to the A2-specific NLVPMVATV peptide. Although the phenotypic pattern of the CMV-specific T cells of the recipient was different from those of the donor before transplantation, the phenotype of the donor-derived cells retained their original phenotype in the recipient after transplantation. These finding suggested that active transferred immunity from the graft with a high frequency of CMV-specific CTL could induce a rapid reconstitution of CMV-specific T-cell mediated immunity in pediatric HLA-identical allogenetic bone marrow transplantation. The screening of peripheral blood using HLA-peptide tetramer staining might be beneficial to select donors.
authors.affiliation: Department of Pediatrics, Sakura Hospital, Toho University, Chiba, Japan. haruki-komatsu@chive.ocn.ne.jp
authors.forename: Haruki
authors.initials: H
authors.lastname: Komatsu
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kogawa
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Nonoyama
authors.forename: Ayno
authors.initials: A
authors.lastname: Inui
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sogo
authors.forename: Tomoo
authors.initials: T
authors.lastname: Fujisawa
authors.forename: Paul
authors.initials: P
authors.lastname: Klenerman
mesh_terms: D001782:Blood Donors; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D015789:HLA-A2 Antigen / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D010641:Phenotype; D035781:Siblings; D013194:Staining and Labeling; D014184:Transplantation, Homologous / Q000009:adverse effects*
chemical_list: D015789:HLA-A2 Antigen; D007371:Interferon-gamma