Molecular characterization of the equine collagen, type IX, alpha 2 (COL9A2) gene on horse chromosome 2p16-->p15.
The mammalian collagen, type IX, alpha 2 gene (COL9A2) encodes the alpha-2 chain of type IX collagen and is located on horse chromosome 2p16-->p14 harbouring a quantitative trait locus for osteochondrosis. We isolated a bacterial artificial chromosome (BAC) clone containing the equine COL9A2 gene and determined the complete genomic sequence of this gene. Cloning and characterization of equine COL9A2 revealed that the equine gene consists of 32 exons spanning approximately 15 kb. The COL9A2 transcript encodes a single protein of 688 amino acids. Thirty two single nucleotide polymorphisms (SNPs) equally distributed in the gene were detected in a mutation scan of eight unrelated Hanoverian warmblood stallions, including one SNP that affects the amino acid sequence of COL9A2. Comparative analyses between horse, human, mouse and rat indicate that the chromosomal location of equine COL9A2 is in agreement with known chromosomal synteny relationships. The comparison of the gene structure and transcript revealed a high degree of conservation towards the other mammalian COL9A2 genes. We chose three informative SNPs for association and linkage disequilibrium tests in three to five paternal half-sib families of Hanoverian warmblood horses consisting of 44 to 75 genotyped animals. The test statistics did not reach the significance threshold of 5% and so we could not show an association of COL9A2 with equine osteochondrosis.
Boneker
C
C
Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Hannover, Germany.
Kuiper
H
H
Drögemüller
C
C
Chowdhary
B P
BP
Distl
O
O
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000235:genetics / Q000648:ultrastructure; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D024081:Collagen Type IX / Q000096:biosynthesis / Q000235:genetics*; D005091:Exons / Q000235:genetics; D020869:Gene Expression Profiling; D005796:Genes / Q000235:genetics; D006734:Horse Diseases / Q000235:genetics; D006736:Horses / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015810:Linkage Disequilibrium; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010007:Osteochondritis / Q000235:genetics / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide; D021101:Radiation Hybrid Mapping; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity
D024081:Collagen Type IX