title: A direct comparison between lamellar body counts and fluorescent polarization methods for predicting respiratory distress syndrome.
abstract: Our objective was to directly compare the diagnostic usefulness of lamellar body counting (LBC) and the TDx-FLM II assay (Abbott Laboratories, Abbott Park, IL) for predicting respiratory distress syndrome (RDS). This was a 5-year, retrospective, cohort study. A diagnosis of RDS was given to infants who received surfactant treatment and/or required ventilator support and/or continuous positive airway pressure for more than 24 hours. There were 172 infants without RDS and 12 with RDS included in the study. By using a TDx-FLM II cutoff of 55 mg/g or more for maturity, the sensitivity was 83%, specificity was 65%, predictive value of a mature result was 98%, and predictive value of an immature result was 14%. These results were similar to LBC using a cutoff of 50,000/microL or more with sensitivity of 92%, a specificity of 60%, a predictive value of a mature result of 99%, and a predictive value of an immature result of 14%. The LBC and TDx-FLM II methods have similar clinical usefulness.
authors.affiliation: Department of Pathology and Immunology, Division of Laboratory Medicine, Washington University School of Medicine, St Louis, MO 63110, USA.
authors.forename: Shannon
authors.initials: S
authors.lastname: Haymond
authors.forename: Veronica I
authors.initials: VI
authors.lastname: Luzzi
authors.forename: Curtis A
authors.initials: CA
authors.lastname: Parvin
authors.forename: Ann M
authors.initials: AM
authors.lastname: Gronowski
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry; D019540:Area Under Curve; D015415:Biomarkers / Q000032:analysis; D015331:Cohort Studies; D005260:Female; D005454:Fluorescence Polarization / Q000379:methods*; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D008297:Male; D011237:Predictive Value of Tests; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D011663:Pulmonary Surfactants / Q000032:analysis*; D012372:ROC Curve; D011933:Reagent Kits, Diagnostic; D012127:Respiratory Distress Syndrome, Newborn / Q000175:diagnosis* / Q000453:epidemiology / Q000628:therapy; D012189:Retrospective Studies
chemical_list: D015415:Biomarkers; D011663:Pulmonary Surfactants; D011933:Reagent Kits, Diagnostic