[Impaired microfilament cytoskeleton rearrangement in cytomegalovirus infected cells].
To investigate the effect of cytomegalovirus (CMV) infection on actin and microfilament in human embryo fibroblast cells (HF) and its relationship with CMV replication.



Cell morphology was observed after the infection of CMV. Western-blot was used to measure the expression levels of beta-actin, G-actin and F-actin proteins. CMV immediately early antigen (CMV IE) in HF cells was analyzed by indirect immunofluorescence assay. Microfilament alteration was determined by cytoskeleton fluorescence probe.



CMV IE was demonstrated in more than 95% of HF cells after infection, which was primarily located in nucleus. The shape of HF cells changed from thin shuttle like to round and thick ball like, even escaping from wall after infection by CMV. Compared with control group, the expression of G-actin protein increased at 24 h of CMV infection (0.941 +/-0.061 compared with 0.714 +/-0.119, P <0.05), then decreased at 72 h, 96 h respectively(0.218 +/-.035, 0.230 +/-0.055 compared with 0.714 +/-0.119, P <0.05). The levels of F-actin in infected cells gradually decreased at 24 h, 72 h and 96 h compared with control HF cells (0.256 +/-0.021, 0.127 +/-0.032, 0.026 +/-0.008 compared with 0.373 +/-0.050, P<0.05). In infected HF cells, microfilaments were found ruptured, arranged turbulently. Cells fused and fluorescence density of microfilament markedly reduced.



Cytomegalovirus can induce alteration of actins and microfilament, which may be associated with its infection, replication and reactivity in host cells.
Jiang
Yan-ling
YL
Department of Hematology, Bone Marrow Transplantation Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. zjhzjyl@zju.edu.cn
Lin
Mao-fang
MF
Zhao
Guang-sheng
GS
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D003587:Cytomegalovirus; D003599:Cytoskeleton / Q000378:metabolism; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism* / Q000648:ultrastructure / Q000821:virology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000032:analysis
D000199:Actins; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus