Isolation and characterization of antifungal substances from Burkholderia sp. culture broth.
A new antagonistic Burkholderia strain, designated MP-1 and producing antifungal activities against various filamentous plant pathogenic fungi, was isolated from the rhizoshere in the Naju area. Cultural characteristic studies strongly suggested that this strain belongs to the genus Burkholderia. The nucleotide sequence of the 16S rRNA gene (1491 pb) of strain MP-1 exhibited close similarity (99% to 100%) with other Burkholderia 16S rRNA genes. Extraction of fermentation broth of Burkholderia sp. MP-1 and various separations and purification steps led to isolation of four pure active molecules. The chemical structure of these four compounds-named phenylacetic acid, hydrocinnamic acid, 4-hydroxyphenylacetic acid, and 4-hydroxyphenylacetate methyl ester-was established on the basis on their gas chromatography-electron impact-mass spectrometry (GC-EI-MS) and trimethylsilation GC-EI-MS data. The four isolated compounds inhibited filamentous fungal growth on potato dextrose agar medium supplemented with 100 mg/L of phenylacetic acid, hydrocinnamic acid, 4-hydroxyphenylacetic acid and 4-hydroxyphenylacetate methyl ester individually.
Mao
Sopheareth
S
Department of Agricultural Chemistry, Division of Applied Bioscience, Environmental-Friendly Agriculture Research Center, Chonnam National University, Yong-Bong Dong, Buk-Ku, Gwangju, South Korea.
Lee
Seung-Je
SJ
Hwangbo
Hoon
H
Kim
Yong-Woong
YW
Park
Keun-Hyung
KH
Cha
Gyu-Suk
GS
Park
Ro-Dong
RD
Kim
Kil-Yong
KY
D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D019117:Burkholderia / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D010648:Phenylacetates / Q000494:pharmacology; D010666:Phenylpropionates / Q000494:pharmacology
D000935:Antifungal Agents; D003470:Culture Media; D010648:Phenylacetates; D010666:Phenylpropionates; C026246:4-hydroxyphenylacetate; C035253:3-phenylpropionic acid; C025136:phenylacetic acid