Comparison of multiple genes of spring viremia of carp viruses isolated in the United States.
Five spring viremia of carp viruses (SVCV), Rhabdovirus carpio, were isolated in the United States (US) between 2002 and 2004. Single tube reverse transcription-polymerase chain reaction (RT-PCR) was used to generate overlapping cDNA fragments from the US isolates of SVCV. Multiple pairs of specific primers were designed to amplify a portion of the phosphoprotein gene, the matrix gene, and the glycoprotein gene of SVCV genogroup Id (corresponding to nucleotides 2174-4942 of GenBank accession NC_002803). Sequences were proofread and aligned to generate a consensus sequence for each isolate. Phylogenetic analysis of the 2705 nucleotide consensus sequence revealed that all five US isolates belong to SVCV genogroup Ia, Asian origin isolates, and a PCR primer binding site unique to SVCV genogroup Ia was identified.
Warg
Janet V
JV
Diagnostic Virology Laboratory, National Veterinary Services Laboratories, VS-APHIS-USDA, Ames, IA 50010, USA. Janet.V.Warg@aphis.usda.gov
Dikkeboom
Audrey L
AL
Goodwin
Andrew E
AE
Snekvik
Kevin
K
Whitney
John
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000821:virology*; D002478:Cells, Cultured; D005393:Fish Diseases / Q000821:virology*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012209:Rhabdoviridae / Q000235:genetics* / Q000302:isolation & purification*; D018353:Rhabdoviridae Infections / Q000821:virology*; D012621:Seasons; D017385:Sequence Homology; D014481:United States; D014766:Viremia / Q000821:virology*