[Effect of delayed preconditioning induced by Sini decoction on myocardial cell apoptosis and its mitochondrial mechanism].
OBJECTIVE
To investigate the mechanism of myocardial cell apoptosis during delayed preconditioning induced by Sini decoction (SND).


METHODS
SD rats were divide into four groups: control, sham, I/R and SND groups. The rats in I/R group, left anterior descending coronary artery (LAD) was occluded for 1h and reperfused for 1 h. The rats in SND group were pretreated with Sini decoction (5 g x kg(-1) x d(-1)) for three days, the last treatment was pretreated 24 h before the index occlusion. Cell apoptosis was measured by flow cytometry, cytochrome C and bcl-xl were detected by Western blotting and the activity of caspase-3 was detected by assay kit.


RESULTS
As compared with I/R group, apoptosis rate of myocardial cell, the release of cytochrome C from mitochondria and the activity of caspase-3 were significantly decreased, and the expression of bcl-xl protein was elevated in SND group.


CONCLUSIONS
The delayed preconditioning induced by Sini decoction decreased myocardial cell apoptosis. The mechanism may be related to the inhibition of mitochondria signal pathway of apoptosis.
Liu
Ying
Y
Department of Pathophysiology of Guangdong Pharmacological College, Guangzhou, China. lylygz@hotmail.com
Wu
Wei-kang
WK
Chen
Chen
C
Duan
Xin-feng
XF
D031841:Aconitum / Q000737:chemistry; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D020939:Zingiber officinale / Q000737:chemistry; D029882:Glycyrrhiza uralensis / Q000737:chemistry; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D051020:bcl-X Protein / Q000378:metabolism
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D051020:bcl-X Protein; D045304:Cytochromes c; D053148:Caspase 3