title: Isolation and identification of a cytopathic activity in Tannerella forsythia.
abstract: Interactions between pathogens and host induce human disorders including periodontitis, disintegration of the tooth supporting tissues. Tannerella forsythia has been linked to the periodontitis and several cytopathic reagents have been found in the bacterium; however, its contribution to the disease remains unclear. Biochemical approach to explore the cytopathic effect revealed two distinct activities in T. forsythia (ATCC 43037) extract; one detaches adherent cells from substratum and another arrests cells at G2. An executor of former activity, forsythia detaching factor (FDF) was identified; its genomic sequence and peptidase activity revealed that FDF is a substantial form of putative PrtH; prtH gene was hypothetically identified directly from a DNA fragment of the bacterium and its native product has never been shown. Since FDF was found in the bacterial culture supernatant, its activity implies a contribution to the disintegration of tissues although the mechanism how FDF disturbs cellular anchors remains elusive.
authors.affiliation: Department of Molecular Cellular Oncology and Microbiology, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-Ku, Tokyo 113-8549, Japan. nakajima.mcom@tmd.ac.jp
authors.forename: Takuma
authors.initials: T
authors.lastname: Nakajima
authors.forename: Naoko
authors.initials: N
authors.lastname: Tomi
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Fukuyo
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishikura
authors.forename: Yuka
authors.initials: Y
authors.lastname: Ohno
authors.forename: Ramanathan
authors.initials: R
authors.lastname: Arvind
authors.forename: Takao
authors.initials: T
authors.lastname: Arai
authors.forename: Isao
authors.initials: I
authors.lastname: Ishikawa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Arakawa
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002277:Carcinoma / Q000473:pathology*; D002457:Cell Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D006801:Humans; D008969:Molecular Sequence Data; D017861:Porphyromonas / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D002457:Cell Extracts