Cloning and characterization of four B-hordein genes from Tibetan hull-less barley (Hordeum vulgare subsp. vulgare).
Four B-hordein genes, designated BH1-BH4, were cloned using PCR amplification from two hull-less barley cultivars, ZQ7239 and ZQ148, collected from Tibet. The results of sequencing indicated that BH1-BH4 contained complete open reading frames (ORFs). Comparison of their predicted polypeptide sequences with the published sequences suggested that they all share the same basic protein structure. Phylogenetic analysis indicated that the deduced amino-acid sequences of BH1-BH4 genes were more closely related to B-hordeins from cultivated barley (Hordeum vulgare L.) than to any other prolamins from wild barley and Aegilops tauschii. Comparison of the coding regions of BH1-BH4 genes showed that BH1 had a lower sequence identity to other previously published B-hordeins than the other three B-hordeins obtained in this study. BH1 was then cloned in a bacterial expression vector based on bacteriophage T7 RNA polymerase. The resulting plasmid produced a 28.15 kDa protein in Escherichia coli. The potential value of B-hordein genes in grain quality improvement of hull-less barley has been discussed.
Han
Zhao-Xue
ZX
Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, China.
Qian
Gang
G
Pan
Zhi-Fen
ZF
Deng
Guang-Bing
GB
Wu
Fang
F
Tang
Ya-Wei
YW
Qiang
Xiao-Lin
XL
Yu
Mao-Qun
MQ
D003001:Cloning, Molecular; D017343:Genes, Plant; D005983:Glutens / Q000235:genetics*; D001467:Hordeum / Q000235:genetics*; D008969:Molecular Sequence Data; D055315:Prolamins / Q000235:genetics; D018609:Tibet
D055315:Prolamins; D005983:Glutens