[Mechanism of serum containing SSTG on calcium overload in cardiomyocytes injuryed by hypoxia and reoxygenation].
OBJECTIVE
To explore the effects and mechanism of Shuangshen Tongguan (SSTG) serum, a formula composed of the active fractions of Chinese medicine, on calcium overload in cultured cardiomyocytes injured by hypoxia and reoxygenation.


METHODS
The cardiomyocytes were deprived of oxygen and glucose to producl hypoxia reoxygenation injuried models. The changes of intracellular calcium fluorescence intensity induced by K+ and Thapsigargin were measured by fluorospectrophotometry and laster scanning confocal microscope respectively.


RESULTS
Intracellular calcium concentration was low in normal cardiomyocytes and was enhanced after hypoxia/reoxygenation (P < 0.05); SSTC drug serum reduced the intracellular calcium concentration and depressed the increase of calcium fluorescence intensity in singular cardiomyocyte due to K+ and Thapsigargin stimulation.


CONCLUSIONS
Hypoxia/reoxygenation injury, K+ and Thapsigargin could induce calcium overload in cardiomyocytes. The effects of calcium antagonism of SSTG drug serum were achieved by inhibiting calcium inflow, promoting calcium re-absorption of calcium.
Liu
Jian-xun
JX
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. jianxun@ht.rol.cn.net
Han
Xiao
X
Xu
Yong-gang
YG
Ma
Rou
R
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D031311:Corydalis / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry; D044967:Serum; D019284:Thapsigargin / Q000037:antagonists & inhibitors
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D019284:Thapsigargin; D002118:Calcium