title: Total hydroperoxide and biological antioxidant potentials in a neonatal sepsis model.
abstract: Oxidant/antioxidant imbalance plays an important role in septic shock. The present study examined changes in circulating oxidative components in a neonatal sepsis model. Subjects were 14 newborn mixed-strain piglets randomly divided into two groups: a cecal ligation and perforation (CLP) model (n = 7) and sham (n = 7). Blood samples for total hydroperoxide (TH), biological antioxidant potential (BAP), tumor necrosis factor (TNF) alpha, interleukin (IL)-6, and IL-10 were collected pre-CLP and at 1, 3, and 6 h post-CLP. TH and BAP levels at 1 h post-CLP were significantly higher in the CLP group than in the sham group. In the CLP group, TH decreased gradually and reached baseline levels by 6 h post-CLP, while BAP remained elevated. Linear correlations were identified between serum TH and BAP at 1 h post-CLP, serum TH and TNF-alpha at 1 h post-CLP, and BAP and IL-6 at 6 h post-CLP. Changes in and correlations between circulating oxidative and inflammatory state components in a neonatal sepsis model were clarified. This is the first study to reveal that the presence of oxidant/antioxidant imbalance in sepsis and septic shock changes during the disease course.
authors.affiliation: Department of Pediatrics, Neonatology and Congenital Disorders, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan. hkaki@med.nagoya-cu.ac.jp
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kakita
authors.forename: Mohamed H
authors.initials: MH
authors.lastname: Hussein
authors.forename: Ghada A
authors.initials: GA
authors.lastname: Daoud
authors.forename: Takenori
authors.initials: T
authors.lastname: Kato
authors.forename: Hiroki
authors.initials: H
authors.lastname: Murai
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sugiura
authors.forename: Keisuke
authors.initials: K
authors.lastname: Mizuno
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Yamada
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ito
authors.forename: Sumio
authors.initials: S
authors.lastname: Fukuda
authors.forename: Ineko
authors.initials: I
authors.lastname: Kato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Suzuki
authors.forename: Hajime
authors.initials: H
authors.lastname: Togari
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism*; D001784:Blood Gas Analysis; D001794:Blood Pressure / Q000502:physiology; D001831:Body Temperature / Q000502:physiology; D004195:Disease Models, Animal; D016905:Gram-Negative Bacterial Infections / Q000378:metabolism* / Q000503:physiopathology; D016908:Gram-Positive Bacterial Infections / Q000378:metabolism* / Q000503:physiopathology; D006861:Hydrogen Peroxide / Q000097:blood*; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides / Q000097:blood; D017382:Reactive Oxygen Species / Q000378:metabolism; D018805:Sepsis / Q000378:metabolism* / Q000382:microbiology; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000975:Antioxidants; D015850:Interleukin-6; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D006861:Hydrogen Peroxide