title: Use of transperineal fine needle aspiration of seminal vesicles to retrieve sperm in a man with obstructive azoospermia.
abstract: OBJECTIVE
  To report the successful and feasible use of transperineal fine needle aspiration of seminal vesicles (FNASV) for sperm retrieval in obstructive azoospermia.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Outpatient care in institutional clinic.
  
  
  METHODS
  A 31-year-old man with obstructive azoospermia due to a middle prostatic müllerian cyst.
  
  
  METHODS
  Transperineal FNASV using a coaxial 17-gauge TruGuide needle.
  
  
  METHODS
  Feasibility of sperm retrieval suitable for future in vitro fertilization.
  
  
  RESULTS
  Transperineal FNASV made it possible to aspirate 11 mL of fluid with a sperm count of 100 million/mL and 15% motility. This sample was cryopreserved for in vitro fertilization using intracytoplasmic sperm injection.
  
  
  CONCLUSIONS
  Transperineal FNASV using a coaxial needle may be a further method for sperm retrieval to add to the repertoire of assisted reproduction technologies.
authors.affiliation: Urology Clinic, Policlinico G. B. Rossi, University of Verona, Verona, Italy. mcerruto@univr.it <mcerruto@univr.it>
authors.forename: Maria Angela
authors.initials: MA
authors.lastname: Cerruto
authors.forename: Giovanni
authors.initials: G
authors.lastname: Novella
authors.forename: Stefano Zecchini
authors.initials: SZ
authors.lastname: Antoniolli
authors.forename: Filiberto
authors.initials: F
authors.lastname: Zattoni
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000473:pathology* / Q000628:therapy*; D044963:Biopsy, Fine-Needle / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D009339:Needles; D012669:Seminal Vesicles / Q000473:pathology* / Q000601:surgery; D020554:Sperm Injections, Intracytoplasmic / Q000295:instrumentation / Q000379:methods; D013094:Spermatozoa / Q000473:pathology* / Q000637:transplantation*; D020858:Tissue and Organ Harvesting / Q000295:instrumentation / Q000379:methods*