title: Recruitment of RNA polymerase to Class II CRP-dependent promoters is improved by a second upstream-bound CRP molecule.
abstract: Genetics and biochemistry have been exploited to investigate transcription activation by the Escherichia coli CRP (cAMP receptor protein) factor at promoters with a DNA site for CRP near position -41 and the effects of a second upstream-bound CRP molecule. We show that the upstream-bound CRP contributes to transcription activation by improving the recruitment of RNA polymerase.
authors.affiliation: School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK.
authors.forename: N S
authors.initials: NS
authors.lastname: Miroslavova
authors.forename: J E
authors.initials: JE
authors.lastname: Mitchell
authors.forename: J
authors.initials: J
authors.lastname: Tebbutt
authors.forename: S J W
authors.initials: SJ
authors.lastname: Busby
mesh_terms: D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D007700:Kinetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics*; D011953:Receptors, Cyclic AMP / Q000235:genetics / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D011953:Receptors, Cyclic AMP; D012319:RNA Polymerase II