Needleless intranasal administration of HVJ-E containing allergen attenuates experimental allergic rhinitis.
Allergic rhinitis (AR) is one of the most common chronic diseases. Although current medications are highly effective in controlling its symptoms, they do not reverse the allergen-specific hypersensitivities that underlie the disease. Immunoglobulin E is a key mediator of AR, and preventing its production is clinically important. In this study, we developed an efficient needleless intranasal protein delivery system using the hemagglutinating virus of Japan envelope vector (HVJ-E). Intranasal delivery of ovalbumin (OVA) once a week for 3 weeks using this system enhanced OVA-induced interferon-gamma production by murine splenocytes. This treatment also attenuated the OVA-induced release interleukin-4 (IL-4) and IL-5 from splenocytes and the production of plasma OVA-specific immunoglobulin E in OVA-sensitive AR model mice. Thus, allergen-containing HVJ-E may be useful for noninvasive treatment of AR.
Yasuoka
Eri
E
Department of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
Oshima
Kazuo
K
Tamai
Katsuto
K
Kubo
Takeshi
T
Kaneda
Yasufumi
Y
D000281:Administration, Intranasal; D000485:Allergens / Q000008:administration & dosage* / Q000276:immunology / Q000627:therapeutic use*; D000818:Animals; D002417:Cattle; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D006262:Health; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000187:drug effects; D009339:Needles; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D012220:Rhinitis / Q000139:chemically induced / Q000276:immunology* / Q000628:therapy*; D029082:Sendai virus / Q000235:genetics*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology; D013997:Time Factors
D000485:Allergens; D016207:Cytokines; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin