title: [Study on determination of entrapment efficiency of sinomenine liposomes].
abstract: OBJECTIVE
  To establish an HPLC method for the determination of entrapment efficiency of sinomenine liposomes.
  
  
  METHODS
  The liposomes and dissociated drugs were separated by sephadex filtration, mini-column centrifugation and dialysis. The methodology study and the optimization of determining condition were carried out at the same time.
  
  
  RESULTS
  Sephadex filtration could effectively separate the sinomenine liposomes from dissociated sinomenine. The column recovery was 98.8%, the average entrapment efficiency of three tests was64.9%, RSD 2.67%.
  
  
  CONCLUSIONS
  The method was simple, exact, and had a good reappearance. It can be used to examine the entrapment efficiency of sinomenine liposomes.
authors.affiliation: Guangzhou University of Traditional Chinese Medicine, Guangzhou, China. zhouliling717@sina.com
authors.forename: Li-ling
authors.initials: LL
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Qing-fei
authors.initials: QF
authors.lastname: Liu
authors.forename: Jia-jun
authors.initials: JJ
authors.lastname: Ling
mesh_terms: D003911:Dextrans; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005374:Filtration / Q000379:methods; D008081:Liposomes; D009019:Morphinans / Q000008:administration & dosage / Q000032:analysis* / Q000302:isolation & purification; D032841:Sinomenium / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D003911:Dextrans; D004337:Drug Carriers; D008081:Liposomes; D009019:Morphinans; C009271:sinomenine; C025614:sephadex