title: Studies on the methylation status of CpG sequences located in promoters of selected tumour suppressor genes in breast cancer cells.
abstract: In the tested samples of sporadic breast cancer (100 cases), hypermethylation of CpG sequences located in ERalpha promoter was observed in 62 cases. It correlated with: (i) deficiency of ERalpha protein in 45%, (ii) hypermethylation of BRCA1 promoter in 95%, and (iii) nonmethylated E-cadherin promoter in 90%. Fifty-eight percent of the patients with nonmethylated E-cadherin promoter (56 cases) did not show metastasis to lymphatic nodes. The analysis of the methylation level of the promoter of ERalpha, BRCA1, and E-cadherin, frequently connected with their activity, shows that it can be an important parameter in the diagnosis and therapeutic strategies in sporadic breast cancer.
authors.affiliation: Department of Biomedicinal Chemistry, Medical University of Lodz, Lodz, Poland. fabian@csk.umed.lodz.pl
authors.forename: K
authors.initials: K
authors.lastname: Fabianowska-Majewska
authors.forename: R
authors.initials: R
authors.lastname: Kordek
authors.forename: B
authors.initials: B
authors.lastname: Krawczyk
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D015820:Cadherins / Q000235:genetics; D018899:CpG Islands; D019175:DNA Methylation; D047628:Estrogen Receptor alpha / Q000235:genetics; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011401:Promoter Regions, Genetic
chemical_list: D015820:Cadherins; D047628:Estrogen Receptor alpha