Monoclonal antibody to the light chain of bovine immunoglobulin.
Monoclonal antibody IVA-285 (IgG1 isotype) recognizing antigenic determinant on bovine and ovine immunoglobulin light chain was produced and characterized. Western blot analysis of bovine immunoglobulin G (IgG) and immunoglobulin M (IgM) purified from bovine blood serum as well as whole immunoglobulin fractions of bovine and ovine serum with IVA-285 showed a molecular weight in the 24-27 kd range corresponding to the Ig light chain of bovine Ig. IVA-285 recognizes the Ig light chain on Ig+ lymphocyte subpopulation and in the majority of body fluids; however, especially strong reactions were observed in bovine tissues (lymph node follicles, plasma cells, and Ig deposits in various tissues).
Antalíková
Jana
J
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic. Jana.Antalikova@savba.sk
Simon
Michal
M
Jankovicová
Jana
J
Horovská
L'ubica
L
Dusinský
Roman
R
Hluchý
Svätoslav
S
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D002417:Cattle; D007074:Immunoglobulin G / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012756:Sheep
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M