title: Effect of oblique nerve grafting on peripheral nerve regeneration in rats.
abstract: Current methods of peripheral nerve repair are to rejoin cut nerve stumps directly or to bridge large gaps with autologous nerve grafts. In both cases the surface of nerve stump endings is typically cut perpendicularly to the long axis of the nerve. The outcome of such operations, however, is still not satisfactory. In this study, we examine the effect of oblique nerve cutting and grafting on morphological as well as functional features of regeneration. In adult rats, sciatic nerve was cut and rejoined either directly or using an autologous graft, at 90 degrees or 30 degrees angle. Functional regeneration was assessed by walking track analysis during 12-week follow-up. Afterwards muscle weight was measured and histological studies were performed. The latter included nerve fibers and Schwann cells counting, as well as visualization of scar formation and epineural fibrosis. Nerves cut obliquely and rejoined showed better functional recovery than perpendicularly transected. Similar effect was observed after oblique grafting when compared to perpendicular one. Numbers of nerve fibers growing into the distal stump of the nerve as well as the number of Schwann cells were significantly higher in obliquely than in perpendicularly operated nerves. Moreover, growing axons were arranged more regularly following oblique treatment. These data indicate that joining or grafting the nerve stumps at acute angle is a more profitable method of nerve repair than the standard procedure performed at right angle.
authors.affiliation: Department of Neurology, Medical University of Silesia, Katowice, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kotulska
authors.forename: Wiesław
authors.initials: W
authors.lastname: Marcol
authors.forename: Magdalena
authors.initials: M
authors.lastname: Larysz-Brysz
authors.forename: Zofia
authors.initials: Z
authors.lastname: Tendera
authors.forename: Izabela
authors.initials: I
authors.lastname: Malinowska-Kołodziej
authors.forename: Wojciech
authors.initials: W
authors.lastname: Slusarczyk
authors.forename: Halina
authors.initials: H
authors.lastname: Jedrzejowska-Szypułka
authors.forename: Joanna
authors.initials: J
authors.lastname: Lewin-Kowalik
mesh_terms: D000818:Animals; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000502:physiology; D012584:Sciatic Nerve / Q000473:pathology / Q000503:physiopathology* / Q000637:transplantation*; D013536:Suture Techniques; D014014:Tissue Adhesives / Q000627:therapeutic use; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014182:Transplantation, Autologous
chemical_list: D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives