title: Role of polymorphonuclear neutrophils (PMNs) and NK cells in the protection conferred by different vaccines against Chlamydophila abortus infection.
abstract: Ovine enzootic abortion (OEA) is caused by Chlamydophila abortus, an intracellular bacterium which acts by infecting the placenta, causing abortion in the last term of gestation. The main prevention strategy against OEA is the vaccination of flocks. An effective vaccine against C. abortus must induce a Th1-like specific immune response, which is characterized by the early production of IFN-gamma and the activation of CD8(+)T cells. Moreover, vaccine effectiveness could be modulated by the functioning of the innate immunity. The purpose of this study was to ascertain how polymorphonuclear neutrophils (PMNs) and NK cells might influence vaccine-induced protection. The live attenuated 1B vaccine and two inactivated experimental vaccines, adjuvated with aluminium hydroxide (AH) or QS-21 (QS), were used in PMN-depleted or NK cell-depleted mice. For PMN depletion, RB6-8C5 monoclonal antibody, which recognizes GR1(+) receptors (Robben, P.M., LaRegina, M., Kuziel, W.A., Sibley, L.D. 2005. Recruitment of Gr-1(+) monocytes is essential for control of acute toxoplasmosis. The Journal of Experimental Medicine 201, 1761-1769.) was used, while for NK cell-depletion the anti-asialo GM1 polyclonal antibody was used. The depletion of PMNs caused 100% mortality in non-vaccinated mice (NV) and 60% mortality in the AH-vaccinated mice by day 10 p.i., while both groups showed a significant increase in their bacterial burden in the liver by day 4 p.i. The depletion of NK cells caused mortality only in the NV group (50% by day 10 p.i.), although this group and the 1B vaccinated mice showed an increased bacterial burden in the liver at day 4 p.i. Our results suggest that the importance of PMNs in inactivated vaccines depends on the adjuvant chosen. The results also demonstrated that the importance of NK cells is greater in live vaccines than in inactivated vaccines.
authors.affiliation: Departamento de Sanidad Animal, Universidad de Murcia, Murcia, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Ortega
authors.forename: M R
authors.initials: MR
authors.lastname: Caro
authors.forename: A J
authors.initials: AJ
authors.lastname: Buendía
authors.forename: M C
authors.initials: MC
authors.lastname: Gallego
authors.forename: L
authors.initials: L
authors.lastname: Del Río
authors.forename: C M
authors.initials: CM
authors.lastname: Martínez
authors.forename: L
authors.initials: L
authors.lastname: Nicolas
authors.forename: F
authors.initials: F
authors.lastname: Cuello
authors.forename: J
authors.initials: J
authors.lastname: Salinas
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D023521:Chlamydophila Infections / Q000276:immunology* / Q000517:prevention & control*; D005260:Female; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology*; D013997:Time Factors
chemical_list: D001428:Bacterial Vaccines