[Determination of propofol in human serum by improved reversed phase high-performance liquid chromatography with fluorescence detection].
OBJECTIVE
To develop a new high-performance liquid chromatographic (HLPC) method for determination of propofol in human serum.


METHODS
Human serum samples were precipitated with 20% perchloric acid and centrifuged to obtain 50 microl of the supernatant for analysis by HPLC coupled with fluorescence detection. The analysis was performed with a C(18) reversed-phase column using a acetonitrile-water (90:10) phase delivered at 1.0 ml/min, with the excitation wavelength of 276 nm and emission wavelength of 310 nm.


RESULTS
The calibration curves were linear (r=0.997 5) within the concentration range of 0.05-10 microg/ml, the limit of propofol quantification was 50 ng/ml and the intra- and inter-day precisions were between -/+15%.


CONCLUSIONS
The method is accurate, sensitive and simple for propofol determination in clinical anesthesia.
Fan
Ying-ying
YY
Department of Anesthesiology, Stomatology Hospital, Fourth Military Medical University, Xi'an 710033, China. fanyingying_229@hotmail.com
Xu
Li-xian
LX
Wen
Ai-dong
AD
Zhang
Hui
H
Liu
Chun-ran
CR
Li
Wei
W
Mei
Xiao-peng
XP
D018686:Anesthetics, Intravenous / Q000097:blood / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D015742:Propofol / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods
D018686:Anesthetics, Intravenous; D015742:Propofol