title: Assessment of genetic stability of Brucella melitensis Rev 1 vaccine strain by multiple-locus variable-number tandem repeat analysis.
abstract: The assessment of the genetic stability is one of the essential elements to guarantee the biological quality of live anti-bacteria vaccines. Live attenuated Brucella melitensis Rev 1 is the most effective vaccine against brucellosis in small ruminants. Thirty-six B. melitensis Rev 1 vaccine strains isolated from human or animal sources from different geographic regions, from different commercial batches or laboratory collections were typed by the multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) recently described for Brucella spp. Our results demonstrated that B. melitensis Rev 1 group as assayed by MLVA is genetically very homogeneous. We believe that MLVA methodology could be an essential assay to guarantee the quality and stability of live anti-bacterial vaccines being produced worldwide and can be included as in vitro control.
authors.affiliation: Departamento de Microbiología y Parasitología, Universidad de Navarra, c/Irunlarrea no. 1, 31008 Pamplona, Spain.
authors.forename: David
authors.initials: D
authors.lastname: García-Yoldi
authors.forename: Philippe
authors.initials: P
authors.lastname: Le Fleche
authors.forename: Clara M
authors.initials: CM
authors.lastname: Marín
authors.forename: María J
authors.initials: MJ
authors.lastname: De Miguel
authors.forename: Pilar M
authors.initials: PM
authors.lastname: Muñoz
authors.forename: Gilles
authors.initials: G
authors.lastname: Vergnaud
authors.forename: Ignacio
authors.initials: I
authors.lastname: López-Goñi
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000235:genetics*; D002002:Brucella / Q000235:genetics* / Q000276:immunology; D002006:Brucellosis / Q000821:virology*; D016000:Cluster Analysis; D042822:Genomic Instability; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D012418:Ruminants
chemical_list: D001428:Bacterial Vaccines; C471349:Rev.1 vaccine, Brucella melitensis