title: Efficient isolation of anthraquinone-derivatives from Trichoderma harzianum ETS 323.
abstract: Anthraquinone-derivatives, chrysophanol and pachybasin, were purified by a silica column chromatography with two different solvent systems from Trichoderma harzianum ETS 323. The fungus was incubated in sugarcane bagasse solid medium at room temperature without rotation. Structure of chrysophanol was solved by X-ray diffraction and pachybasin by NMR spectra. About 233+/-13 mg of pure chrysophanol and 773+/-40 mg of pure pachybasin were recovered per kg of solid cultural medium, with yields 1.7+/-0.2% and 5.6+/-0.5%, respectively.
authors.affiliation: Department of Molecular Biotechnology, Da-Yeh University, Changhua 51591, Taiwan.
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Liu
authors.forename: Chaur-Tsuen
authors.initials: CT
authors.lastname: Lo
authors.forename: Chinpiao
authors.initials: C
authors.lastname: Chen
authors.forename: Ming-Yih
authors.initials: MY
authors.lastname: Liu
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Chen
authors.forename: Kou-Cheng
authors.initials: KC
authors.lastname: Peng
mesh_terms: D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D018360:Crystallography, X-Ray; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D031786:Saccharum / Q000382:microbiology; D012822:Silicon Dioxide; D014242:Trichoderma / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000880:Anthraquinones; D000935:Antifungal Agents; D012822:Silicon Dioxide; C027113:chrysophanic acid