Constructing a novel Nanodevice powered by delta-free FoF1-ATPase.
A Nanodevice was constructed by delta-free F(o)F(1)-ATPase within chromatophores and actin filaments through biotinlipid-streptavidin-biotin-(AC(5))(2)Sulfo-OSu system. One actin filament linking with many chromatophores functions as the Nanodevice body and many delta-free F(o)F(1)-ATPase as the Nanodevice motors. Movement of the Nanodevice was observed directly by fluorescence microscopy with CCD camera after illumination. The moving speed was about 2.17-24.43mum/s for various length Nanodevices and most of them were stopped by adding CCCP. This means that the Nanodevice was driven by PMF (proton-motive force) in the cooperating delta-free F(o)F(1)-ATPase. From bioengineering point of view, the cooperation of F(o)F(1)-ATPase is a very important research field in the future.
Su
Ting
T
National Laboratory of Biomacromolecules, Institute of Biophysics, CAS, 15 Datun Road, Chaoyang District, Beijing 100101, PR China.
Cui
Yuanbo
Y
Zhang
Xiaoai
X
Liu
Xiaolong
X
Yue
Jiachang
J
Liu
Ning
N
Jiang
Peidong
P
D000199:Actins; D002856:Chromatophores / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D018499:Linear Energy Transfer; D020409:Molecular Motor Proteins / Q000737:chemistry*; D009038:Motion; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D006180:Proton-Translocating ATPases / Q000737:chemistry*
D000199:Actins; D020409:Molecular Motor Proteins; D006180:Proton-Translocating ATPases