Secretion of functional monocyte chemotactic protein 3 by recombinant Mycobacterium bovis BCG attenuates vaccine virulence and maintains protective efficacy against M. tuberculosis infection.
A strain of Mycobacterium bovis BCG that secretes high levels of functional murine monocyte chemotactic protein 3 (BCG(MCP-3)) was developed. Mice vaccinated with BCG(MCP-3) displayed increased lymphocyte migration in vivo and augmented antigen-specific T-cell responses compared to mice vaccinated with BCG alone. The level of protection afforded by BCG(MCP-3) was equivalent to that with control BCG; however, immunodeficient mice infected with BCG(MCP-3) survived significantly longer than mice infected with the control BCG strain. Therefore, BCG(MCP-3) may be a safer alternative than conventional BCG for vaccination of immunocompromised individuals.
Ryan
Anthony A
AA
Mycobacterial Research Group, Centenary Institute of Cancer Medicine and Cell Biology, Locked Bag No. 6, Newtown, NSW 2042, Australia.
Spratt
Joanne M
JM
Britton
Warwick J
WJ
Triccas
James A
JA
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D054410:Chemokine CCL7; D005260:Female; D016867:Immunocompromised Host; D051379:Mice; D008810:Mice, Inbred C57BL; D018945:Monocyte Chemoattractant Proteins / Q000276:immunology* / Q000378:metabolism; D009163:Mycobacterium bovis / Q000276:immunology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D014376:Tuberculosis / Q000276:immunology* / Q000517:prevention & control*
D001500:BCG Vaccine; C515451:Ccl7 protein, mouse; D054410:Chemokine CCL7; D018945:Monocyte Chemoattractant Proteins; D011994:Recombinant Proteins