title: [Cannabinoid receptor 1 expression and pathological changes in rat hippocampus after deprivation of rapid eye movement sleep].
abstract: To observe the expression of cannabinoid receptor 1 (CB1R) mRNA and pathological changes in rat hippocampus after deprivation of rapid eye movement (REM) sleep.
  
  
  
  Totally 42 Sprague-Dawley male rats were randomly divided into cage control (CC), tank control (TC) and the sleep deprivation groups (SD). The SD and TC rats were sacrificed at the end of 1 d, 3 d and 5 d sleep deprivation periods, respectively. The modified multiple platform methods were established for the REM sleep deprivation. CB1R mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR). The hippocampus sections of different stages were observed with electron microscope.
  
  
  
  In SD 1 d group, the expression of CB1R mRNA was significantly increased compared with the CC, TC, SD 3 d and SD 5 d groups (P <0.05) while in SD 3 d group it was reduced. The expression of CB1R mRNA of SD 5 d group was significantly higher than that of the SD 3 d group (P <0.05). Neuron apoptosis was found in SD 3 d and SD 5 d groups.
  
  
  
  Sleep deprivation can cause brain injury with the changes of CB1R mRNA expression.
authors.affiliation: The Affiliated Children Hospital, College of Medicine, Zhejianng University, Hangzhou 310003, China. jpf6688@163.com
authors.forename: Pei-fang
authors.initials: PF
authors.lastname: Jiang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhu
authors.forename: Zhe-zhi
authors.initials: ZZ
authors.lastname: Xia
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000378:metabolism* / Q000648:ultrastructure*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D043884:Receptor, Cannabinoid, CB1 / Q000096:biosynthesis* / Q000235:genetics; D012892:Sleep Deprivation / Q000378:metabolism / Q000473:pathology; D012895:Sleep, REM
chemical_list: D012333:RNA, Messenger; D043884:Receptor, Cannabinoid, CB1