title: [Study on fingerprints of astragalosides injection by HPLC-ELSD].
abstract: OBJECTIVE
  To develop an HPLC method for fingerprint determination of the astragalosides injection.
  
  
  METHODS
  Analyses were carried out at 25 degrees C on a Zorbax SB-C18 column (4.6 mm x 250 mm,5 microm). Mobile phase A was water, and B was acetonitrile. The analysis followed a linear gradient program. Initial conditions were 15% B; 0 to approximately 65 min, changed to 60% B. The flow rate was 0.8 mL x min(-1). The drift tube temparature of the ELSD was 105 degrees C, and gas flowrate was 2.7 L x min(-1).
  
  
  RESULTS
  The RSD of relative retention time of the common peaks in the plant material, the extract and the injection fingerprints was less than 0.1%, and there was good similarity among 10 batches of samples.
  
  
  CONCLUSIONS
  The method was reliable and simple which could be used for quality control of the injection.
authors.affiliation: Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Guan
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Bi
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D027885:Astragalus propinquus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D007267:Injections; D008027:Light; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012503:Saponins / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D012542:Scattering, Radiation; D014315:Triterpenes / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A