title: Escherichia coli is naturally transformable in a novel transformation system.
abstract: A novel transformation system, in which neither a nonphysiological concentration of Ca2+ and temperature shifts nor electronic shocks were required, was developed to determine whether Escherichia coli is naturally transformable. In the new protocol, E. coli was cultured normally to the stationary phase and then cultured statically at 37 degrees C in Luria-Bertani broth. After static culture, transformation occurred in bacteria spread on Luria-Bertani plates. The protein synthesis inhibitor chloramphenicol inhibited this transformation process. The need for protein synthesis in plated bacteria suggests that the transformation of E. coli in this new system is regulated physiologically.
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Dongchang
authors.initials: D
authors.lastname: Sun
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Mei
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Xie
authors.forename: Huihui
authors.initials: H
authors.lastname: Liu
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Shen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D013696:Temperature; D014169:Transformation, Bacterial / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media