Enhanced immune responses induced by vaccine using Sendai virosomes as carrier.
Sendai virosomes can deliver encapsulated contents into the cytoplasm directly in a virus fusion-dependent manner. In this paper, Sendai virosomes-formulated melanoma vaccine was constructed and its anti-tumor effects were investigated. The melanoma vaccine was prepared by encapsulating mixture antigen into the Sendai virosomes. The antigen, mixture proteins were extracted from B(16) melanoma cells. The cytotoxic T lymphocyte (CTL) response level was evaluated by (51)Cr release method, and the change of CD4(+) and CD8(+) expression as well as the concentration of IgG in serum of immunized mice was measured. The results showed that Sendai virosomes-formulated melanoma vaccine can effectively elicit not only systemic immune response but also strong CTL response. Sendai virosomes can be used as an effective vector for use in anti-tumor vaccine therapy.
Li
Qiang
Q
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, PR China.
Gao
Jian-Qing
JQ
Qiu
Li-Yan
LY
Cui
Fu-De
FD
Jin
Yi
Y
D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000627:therapeutic use; D016503:Drug Delivery Systems; D005260:Female; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D029082:Sendai virus / Q000276:immunology*; D022701:Virosomes / Q000008:administration & dosage / Q000276:immunology*
D019496:Cancer Vaccines; D022701:Virosomes