Immune response in mice and cattle after immunization with a Boophilus microplus DNA vaccine containing bm86 gene.
Plasmid pBMC2 encoding antigen Bm86 from a Colombian strain of cattle tick Boophilus microplus, was used for DNA-mediated immunization of BALB/c mice, employing doses of 10 and 50microg, delivered by intradermic and intramuscular routes. Anti-Bm86 antibody levels were significantly higher compared to control mice treated with PBS. In the evaluation of immunoglobulin isotypes, significant levels of IgG2a and IgG2b were observed in mice immunized with 50microg of pBMC2. Measurement of interleukine (IL) levels (IL-4, IL-5, IL-12(p40)) and interferon-gamma (IFN-gamma) in the sera of mice immunized with pBMC2 indicated high levels of IL-4 and IL-5, although there were also significant levels of IFN-gamma. Mice immunized with pBMC2 showed antigen-specific stimulation of splenocytes according to the incorporation of bromodeoxyuridine and IFN-gamma secretion. In all trials, mice injected intramuscularly with 50microg of pBMC2 presented the highest immune response. Moreover, cattle immunized with this DNA vaccine showed antibody production significantly different to the negative control. In conclusion, these results suggest the potential of DNA immunization with pBMC2 to induce humoral and cellular immune responses against B. microplus.
Ruiz
Lina María
LM
Unidad de Biotecnología y Control Biológico, Corporación para Investigaciones Biológicas, Medellín, Colombia.
Orduz
Sergio
S
López
Elkin D
ED
Guzmán
Fanny
F
Patarroyo
Manuel E
ME
Armengol
Gemma
G
D000818:Animals; D000906:Antibodies / Q000097:blood; D000917:Antibody Formation / Q000276:immunology*; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D007111:Immunity, Cellular / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology*; D013983:Tick Control / Q000379:methods; D013984:Tick Infestations / Q000517:prevention & control / Q000662:veterinary*; D014612:Vaccines / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
D000906:Antibodies; C085065:BM86 protein, Boophilus; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014612:Vaccines; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic