title: Investigation by bioassay of the efficacy of sodium hydroxide treatment on the inactivation of mouse-adapted scrapie.
abstract: Sodium hydroxide (NaOH) has been shown to reduce the infectivity of transmissible spongiform encephalopathy (TSE) agents. This study investigated the efficacy of sodium hydroxide at 0.1M, 0.25M and 0.5M concentrations for the inactivation of mouse-adapted scrapie strain ME7. Times and temperatures modelled conditions used in an industrial plasma fractionation plant for sanitisation of ultrafilters, and the sodium hydroxide component of Clean In Place sanitisation. The concentration of scrapie ME7 brain homogenate in NaOH test solutions was 1% (w/v). At the end of incubation periods, the samples were adjusted to neutral pH prior to intracerebral inoculation into mice for bioassay. The conditions of 0.1M NaOH at 60 degrees C for 2min and 0.25M NaOH at 30 degrees C for 60min were found to inactivate 3.96 and 3.93logs of scrapie, respectively. Use of 0.5M NaOH at 30 degrees C for 60 or 75min was found to inactivate >or=4.23 and 4.15logs of scrapie. This indicates that the use of these conditions in an industrial process would substantially reduce prion infectivity.
authors.affiliation: CSL Bioplasma, 189-209 Camp Road, Broadmeadows, Victoria 3047, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Unal
authors.forename: J
authors.initials: J
authors.lastname: Thyer
authors.forename: E
authors.initials: E
authors.lastname: Uren
authors.forename: D
authors.initials: D
authors.lastname: Middleton
authors.forename: M
authors.initials: M
authors.lastname: Braun
authors.forename: D
authors.initials: D
authors.lastname: Maher
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001681:Biological Assay / Q000379:methods; D001688:Biological Products / Q000009:adverse effects / Q000302:isolation & purification; D002417:Cattle; D003666:Decontamination / Q000379:methods; D051379:Mice; D018620:PrPSc Proteins / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D012449:Safety; D012608:Scrapie / Q000517:prevention & control / Q000635:transmission; D012756:Sheep; D012972:Sodium Hydroxide / Q000494:pharmacology*; D013696:Temperature
chemical_list: D001688:Biological Products; D018620:PrPSc Proteins; D012972:Sodium Hydroxide