Compounds with DNA cleaving activity from Kadsura ananosma.
Two DNA cleavage agents, meso-dihydroguaiaretic acid (1) and isobavachalcone (2) together with the known alpha-ylangene, beta-sitosterol, daucosterol, pentacosane, hexacosanic acid and cerotic acid 1-monoglyceride were isolated from the stem barks of Kadsura ananosma Kerr for the first time. Compounds 1 and 2 showed relaxation of supercoiled DNA to nicked DNA. 1 represented a new structural type of DNA cleavage agent, while 2 was reported to show DNA strand-scission activity for the first time. 1 also showed significant cytotoxic effect on Hela and Leukemia cells in vitro.
Chen
Ye-Gao
YG
Department of Chemistry, Yunnan Normal University, China. ygchen48@hotmail.com
Song
Xiao-Ping
XP
Hai
Li-Na
LN
Lv
Yu-Ping
YP
Fang
A
A
Halaweish
F
F
Liao
Xin-Rong
XR
D045744:Cell Line, Tumor; D047188:Chalcones / Q000737:chemistry* / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004278:DNA, Superhelical / Q000737:chemistry / Q000187:drug effects; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D032135:Kadsura / Q000737:chemistry*; D007938:Leukemia / Q000188:drug therapy / Q000473:pathology; D017705:Lignans / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009690:Nucleic Acid Conformation; D024301:Plant Bark / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
D047188:Chalcones; D004278:DNA, Superhelical; D017705:Lignans; C468754:isobavachalcone; C015816:dihydroguaiaretic acid; D006139:Guaiacol; D004247:DNA