Pathological effects of the mushroom toxin alpha-amanitin on BALB/c mice.
The pathological effects of alpha-amanitin on BALB/c mice after receiving intravenous injection were evaluated by RP-HPLC and mouse genome oligonucleotide microarray. The content of alpha-amanitin in Amanita virosa was about 2833.8 microg/g dry fruiting body. The liver and kidneys showed critical pathological changes after alpha-amanitin poisoning, and sera BUN, Crea, ALT, AST, TBIL and DBIL were the sensitive markers. The compound alpha-amanitin was detected in liver and kidney tissue homogenates by RP-HPLC after 48 h. The results of mouse genome oligonucleotide microarray showed 146 genes' expression changed, which formed the alternant network. The expression of 66 genes decreased, while 80 ones increased with more than two-fold differential expression after 48 h. The compound alpha-amanitin influenced not only RNA polymerase II, but also the expression of its associated genes. The application of mouse oligo chip provided valuable data for further understanding the biological properties and molecular pathogenesis of alpha-amanitin, also might be helpful for screening the curative drug for alpha-amanitin intoxication.
Zhao
Jian
J
Division of Cancer Biotherapy, State Key Laboratory of Biotherapy, West China Medical School, Sichuan University, Chengdu 610064, PR China.
Cao
Mei
M
Zhang
Jie
J
Sun
Qun
Q
Chen
Qian
Q
Yang
Zhi-Rong
ZR
D000545:Amanita / Q000737:chemistry*; D000546:Amanitins / Q000633:toxicity*; D000818:Animals; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009145:Mushroom Poisoning / Q000209:etiology* / Q000378:metabolism / Q000473:pathology*; D011897:Random Allocation
D000546:Amanitins