Population genetic structure of the dengue mosquito Aedes aegypti in Venezuela.
The mosquito Aedes aegypti is the main vector of dengue in Venezuela. The genetic structure of this vector was investigated in 24 samples collected from eight geographic regions separated by up to 1160 km. We examined the distribution of a 359-basepair region of the NADH dehydrogenase subunit 4 mitochondrial gene among 1144 Ae. aegypti from eight collections. This gene was amplified by the polymerase chain reaction and tested for variation using single strand conformation polymorphism analysis. Seven haplotypes were detected throughout Venezuela and these were sorted into two clades. Significant differentiation was detected among collections and these were genetically isolated by distance.
Herrera
Flor
F
Centro de Investigaciones Biomédicas, Universidad de Carabobo Callejón Cecilio Acosta, Avda. Las Delicias, Maracay 2101, Aragua, Venezuela. flormhq@cantv.net
Urdaneta
Ludmel
L
Rivero
José
J
Zoghbi
Normig
N
Ruiz
Johanny
J
Carrasquel
Gabriela
G
Martínez
José Antonio
JA
Pernalete
Martha
M
Villegas
Patricia
P
Montoya
Ana
A
Rubio-Palis
Yasmin
Y
Rojas
Elina
E
D000330:Aedes / Q000201:enzymology / Q000235:genetics*; D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D003715:Dengue / Q000635:transmission; D014644:Genetic Variation / Q000235:genetics*; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D007303:Insect Vectors / Q000201:enzymology / Q000235:genetics*; D009245:NADH Dehydrogenase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D014687:Venezuela
D004272:DNA, Mitochondrial; C098483:NADH dehydrogenase subunit 4; D009245:NADH Dehydrogenase