[Study on determination of entrapment efficiency of sinomenine liposomes].
OBJECTIVE
To establish an HPLC method for the determination of entrapment efficiency of sinomenine liposomes.


METHODS
The liposomes and dissociated drugs were separated by sephadex filtration, mini-column centrifugation and dialysis. The methodology study and the optimization of determining condition were carried out at the same time.


RESULTS
Sephadex filtration could effectively separate the sinomenine liposomes from dissociated sinomenine. The column recovery was 98.8%, the average entrapment efficiency of three tests was64.9%, RSD 2.67%.


CONCLUSIONS
The method was simple, exact, and had a good reappearance. It can be used to examine the entrapment efficiency of sinomenine liposomes.
Zhou
Li-ling
LL
Guangzhou University of Traditional Chinese Medicine, Guangzhou, China. zhouliling717@sina.com
Wang
Yan
Y
Liu
Qing-fei
QF
Ling
Jia-jun
JJ
D003911:Dextrans; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005374:Filtration / Q000379:methods; D008081:Liposomes; D009019:Morphinans / Q000008:administration & dosage / Q000032:analysis* / Q000302:isolation & purification; D032841:Sinomenium / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods
D003911:Dextrans; D004337:Drug Carriers; D008081:Liposomes; D009019:Morphinans; C009271:sinomenine; C025614:sephadex