title: The effect of fluoride at plaque fluid concentrations on enamel de- and remineralisation at low pH.
abstract: The aim was to study the effect of fluoride, at concentrations typical of plaque fluid, on de- and remineralisation of subsurface lesions at low pH. Artificial lesions in human enamel were microradiographed to quantify mineral loss and placed in acid-gel systems at pH 4.8, 5.0 and 5.2. Calcium and phosphate were added to give initial Ca and Pi concentrations of either 4.1 and 8.0 mM, or 4.7 and 9.7 mM, at each pH value. Further, at each pH and combination of Ca and Pi, fluoride was added to the gels to give initial concentrations of 1, 2 or 5 ppm, with a non-fluoride control group. The lesions were removed after 10 days and change in mineral content quantified. Those in the non-fluoride control groups had demineralised further. Those exposed to fluoride had remineralised, the amount increasing with increasing fluoride concentration, up to a maximum value of approximately 75%. Calcium activity in the gels was reduced significantly, to levels similar to those reported for plaque fluid at low pH. Fluoride activity was also reduced, though to a lesser extent. These findings contrast with those from studies which have simulated conditions on smooth surface sites and which used experimental solutions composed to reflect salivary fluoride concentrations, where net demineralisation was observed at low pH. This reflects the need for further study of de- and remineralisation under plaque-fluid conditions. In conclusion, subsurface lesions were remineralised at low pH by fluoride at concentrations found in plaque fluid during a cariogenic challenge.
authors.affiliation: Unilever Oral Care, Bebington, Wirral, UK. richard.lynch.unilever.com
authors.forename: R J M
authors.initials: RJ
authors.lastname: Lynch
authors.forename: U
authors.initials: U
authors.lastname: Mony
authors.forename: J M
authors.initials: JM
authors.lastname: Ten Cate
mesh_terms: D000328:Adult; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D002327:Cariostatic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D003743:Dental Enamel / Q000000981:diagnostic imaging / Q000187:drug effects*; D003773:Dental Plaque / Q000517:prevention & control*; D005459:Fluorides / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D011859:Radiography; D017001:Tooth Demineralization / Q000188:drug therapy*; D014089:Tooth Remineralization
chemical_list: D002327:Cariostatic Agents; D005459:Fluorides; D002118:Calcium