Diagnostic challenges for multiplexed protein microarrays.
Multiplexed protein analysis using planar microarrays or microbeads is growing in popularity for simultaneous assays of antibodies, cytokines, allergens, drugs and hormones. However, this new assay format presents several new operational issues for the clinical laboratory, such as the quality control of protein-microarray-based assays, the release of unrequested test data and the use of diagnostic algorithms to transform microarray data into diagnostic results.
Master
Stephen R
SR
Department of Pathology & Laboratory Medicine, University of Pennsylvania Medical Center, 3400 Spruce Street, Philadelphia, PA 19104, USA.
Bierl
Charlene
C
Kricka
Larry J
LJ
D000465:Algorithms; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D035843:Biomedical Research / Q000941:ethics; D003219:Confidentiality / Q000941:ethics; D003627:Data Interpretation, Statistical; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000941:ethics; D011237:Predictive Value of Tests; D040081:Protein Array Analysis / Q000941:ethics / Q000379:methods; D011506:Proteins / Q000032:analysis; D011786:Quality Control; D011933:Reagent Kits, Diagnostic / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015415:Biomarkers; D011506:Proteins; D011933:Reagent Kits, Diagnostic