Cell cycle dependent regulation of deoxycytidine kinase, deoxyguanosine kinase, and cytosolic 5'-nucleotidase I activity in MOLT-4 cells.
Activation of nucleoside analogues is dependent on kinases and 5'-nucleotidases and the balance between the activity of these enzymes. The purpose of this study was to analyze deoxycytidine kinase, deoxyguanosine kinase, and 4 different 5'-nucleotidases during cell cycle progression in MOLT-4 cells. The activity of both kinases was cell cycle dependent and increased during proliferation while the activity of cytosolic 5'-nucleotidase I decreased. We could show that the kinase activity was higher than the total nucleotidase activity, which was unchanged or decreased during cell cycle progression. These data may be important in designing modern combination therapy with nucleoside analogues.
Fyrberg
A
A
Department of Medicine and Care, Clinical Pharmacology, Faculty of Health Sciences, Linköping University, Linköping, Sweden. anfyr@imv.liu.se
Mirzaee
S
S
Lotfi
K
K
D015720:5'-Nucleotidase / Q000096:biosynthesis*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology*; D003842:Deoxycytidine Kinase / Q000096:biosynthesis*; D005456:Fluorescent Dyes / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020128:Inhibitory Concentration 50; D009705:Nucleosides / Q000737:chemistry; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors
D016895:Culture Media, Serum-Free; D005456:Fluorescent Dyes; D009705:Nucleosides; D013778:Tetrazolium Salts; D013844:Thiazoles; D017853:Phosphotransferases (Alcohol Group Acceptor); C019165:deoxyguanosine kinase; D003842:Deoxycytidine Kinase; D015720:5'-Nucleotidase; C022616:thiazolyl blue