pH- and temperature-sensitive, injectable, biodegradable block copolymer hydrogels as carriers for paclitaxel.
Paclitaxel (PTX) was loaded into synthetic pH/T-sensitive block copolymer (OSM-PCLA-PEG-PCLA-OSM) solution with various concentrations. The phase diagram of PTX-loaded block copolymer solution shifted to lower temperature region compared to net block copolymer because of the salting-out effect of PTX. Release profiles of PTX showed sustained manner regardless of loading amount of PTX. To evaluate anti-tumor effect of PTX-loaded block copolymer, solutions were injected subcutaneously to tumor-bearing mice and TUNEL assay examined. PTX-loaded block copolymer hydrogel for in vivo use showed good anti-tumor effect for 2 weeks and induced strong apoptosis in tumor tissue. Therefore, we conclude OSM-PCLA-PEG-PCLA-OSM block copolymer as an effective injectable carrier of PTX.
Shim
Woo Sun
WS
Department of Polymer Science & Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Kim
Jong-Ho
JH
Kim
Kwangmeyung
K
Kim
Yoo-Shin
YS
Park
Rang-Woon
RW
Kim
In-San
IS
Kwon
Ick Chan
IC
Lee
Doo Sung
DS
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D020287:In Situ Nick-End Labeling; D007279:Injections, Subcutaneous; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D017239:Paclitaxel / Q000737:chemistry* / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry*; D012878:Skin Neoplasms / Q000188:drug therapy / Q000473:pathology; D013696:Temperature
D000972:Antineoplastic Agents, Phytogenic; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D020100:Hydrogels; D011108:Polymers; D017239:Paclitaxel