Screening for motility-associated genes in malignant astrocytoma cell lines.
The most characteristic feature of a malignant astrocytoma is its early and extensive infiltration into adjacent parenchymal structures. We focused on detecting the possible expression changes as the determining factors for malignant astrocytoma's motile ability. We confirmed that four of 39 genes showed different expression on DD-PCR by RT-PCR and Northern blot analysis. These findings suggest that the genes identified may be important for determining high motility in astrocytoma cell lines. These findings may help us understand the molecular invasion mechanism in astrocytomas.
Ryu
Hyang-Hwa
HH
Brain Tumor Research Laboratory, Medical School, Chonnam National University, Gwangju, and Department of Neurosurgery, Chonnam National University Hwasun Hospital, Jeollanam-do, Republic of Korea.
Jung
Shin
S
Sun
Heung-Suk
HS
Jung
Tae-Young
TY
Jin
Shu-Guang
SG
Jin
Yong-Hao
YH
Kim
In-Young
IY
Jeong
Young-Il
YI
Kang
Sam-Suk
SS
D001254:Astrocytoma / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics*; D002465:Cell Movement; D020869:Gene Expression Profiling; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor