Highly selective and sensitive template-directed photoligation of DNA via 5-carbamoylvinyl-2'-deoxycytidine.
We describe a highly efficient template-directed photoligation of oligodeoxynucleotides (ODNs) through 5-carbamoylvinyl-2'-deoxycytidine ((CV)C). When an ODN containing (CV)C at the 5' end was photoirradiated with an ODN containing a pyrimidine base at the 3' end in the presence of template DNA, efficient photoligation was observed without any byproduct formation. Single nucleotide differences can be successfully distinguished by using photoligation-based DNA chip assay. [structure: see text]
Yoshimura
Yoshinaga
Y
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
Okamura
Daisuke
D
Ogino
Masayuki
M
Fujimoto
Kenzo
K
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000737:chemistry*; D005453:Fluorescence; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry
C521220:5-carbamoylvinyl-2'-deoxycytidine; D009841:Oligonucleotides; D003841:Deoxycytidine; D004247:DNA