title: Uniformly-sized, molecularly imprinted polymers for (-)-epigallocatechin gallate, -epicatechin gallate and -gallocatechin gallate by multi-step swelling and polymerization method.
abstract: Uniformly-sized, molecularly imprinted polymers (MIPs) for (-)-epigallocatechin gallate (EGCg), -epicatechin gallate (ECg) and -gallocatechin gallate (GCg) were prepared by a multi-step swelling and polymerization method using 2-vinylpyridine as a functional monomer, ethylene glycol dimethacrylate as a cross-linker and cyclohexanol as a porogen. Molecular recognition abilities of the obtained MIPs were evaluated in liquid chromatography using a mixture of ethanol and water, or ethanol as the eluent. Each MIP gave the highest molecular recognition ability for the respective template molecule. In addition, (-)-EGCg and -ECg had the same configuration (2R,3R) at positions 2 and 3, and therefore resulting in high cross reactivity each other. However, (-)-GCg, which has different configuration at position 2 with (-)-EGCg and -ECg, showed low cross reactivity with them. On the other hand, those MIPs showed no molecular recognition against (-)-epigallocatechin and -epicatechin, which have no gallate group at position 3. These results indicate that the MIPs prepared can recognize configuration at position 2 and a gallate group at position 3. Furthermore, the MIP for (-)-GCg could be successfully used for isolating (-)-EGCg and -ECg from green tea extract.
authors.affiliation: Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan. haginaka@mukogawa-u.ac.jp
authors.forename: Jun
authors.initials: J
authors.lastname: Haginaka
authors.forename: Hiromi
authors.initials: H
authors.lastname: Tabo
authors.forename: Masaki
authors.initials: M
authors.lastname: Ichitani
authors.forename: Takanobu
authors.initials: T
authors.lastname: Takihara
authors.forename: Akio
authors.initials: A
authors.lastname: Sugimoto
authors.forename: Haruyo
authors.initials: H
authors.lastname: Sambe
mesh_terms: D002392:Catechin / Q000031:analogs & derivatives* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013662:Tea / Q000737:chemistry
chemical_list: D013662:Tea; C417940:gallocatechin gallate; D002392:Catechin; C062669:epicatechin gallate; C045651:epigallocatechin gallate