title: Impaired function of dendritic cells in alymphoplasia (aly/aly) mice for expansion of CD25+CD4+ regulatory T cells.
abstract: Alymphoplasia (aly/aly) mice are from a naturally occurring strain with a mutation in nuclear factor-kappa B inducing kinase (NIK). The NIK mutation causes disruption of the architecture of the thymus and spleen and aly/aly mice show decreased numbers of CD25+CD4+T cells in the spleen. For the expansion of CD25+CD4+T cells, interactions between dendritic cells (DCs) and CD25+CD4+ regulatory T cells are necessary. We investigated the ability of DCs to induce expansion of CD25+CD4+T cells. We found that DCs are reduced in the spleen of aly/aly mice, and showed low expressions of CD80, CD86 and MHC class II molecules on the surface. DCs from aly/aly mice showed decreased ability to present ovalbumin (OVA) to T cells from OVA specific TCR transgenic mice, and a decreased ability for alloantigen presentation. Further, DCs showed a decreased ability to induce expansion of CD25+CD4+T cells in vitro. Our results suggested that the impairment of DCs in aly/aly mice is responsible, at least in part, for the decreased numbers of CD25+CD4+T cells in the periphery of aly/aly mice.
authors.affiliation: Department of Immunology, Yokohama City University School of Medicine, Yokohama, Japan.
authors.forename: Chizuru
authors.initials: C
authors.lastname: Tamura
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nakazawa
authors.forename: Masaki
authors.initials: M
authors.lastname: Kasahara
authors.forename: Chie
authors.initials: C
authors.lastname: Hotta
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yoshinari
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Sato
authors.forename: Mutsuhiko
authors.initials: M
authors.lastname: Minami
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D018122:B7-1 Antigen / Q000276:immunology; D051940:B7-2 Antigen / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology*; D005802:Genes, MHC Class II; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D008206:Lymphatic Diseases / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000473:pathology*
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit; D010047:Ovalbumin