title: Annexin A2 recognises a specific region in the 3'-UTR of its cognate messenger RNA.
abstract: Annexin A2 is a multifunctional Ca(2+)- and lipid-binding protein. We previously showed that a distinct pool of cellular Annexin A2 associates with mRNP complexes or polysomes associated with the cytoskeleton. Here we report in vitro and in vivo experiments showing that Annexin A2 present in this subset of mRNP complexes interacts with its cognate mRNA and c-myc mRNA, but not with beta(2)-microglobulin mRNA translated on membrane-bound polysomes. The protein recognises sequence elements within the untranslated regions, but not within the coding region, of its cognate mRNA. Alignment of the Annexin A2-binding 3'-untranslated regions of annexin A2 mRNA from several species reveals a five nucleotide consensus sequence 5'-AA(C/G)(A/U)G. The Annexin A2-interacting region of the 3'-untranslated region can be mapped to a sequence of about 100 nucleotides containing two repeats of the consensus sequence. The binding elements appear to involve both single and double stranded regions, indicating that a specific higher order mRNA structure is required for binding to Annexin A2. We suggest that this type of interaction is representative for a group of mRNAs translated on cytoskeleton-bound polysomes.
authors.affiliation: Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway.
authors.forename: Hanne
authors.initials: H
authors.lastname: Hollås
authors.forename: Ingvild
authors.initials: I
authors.lastname: Aukrust
authors.forename: Stine
authors.initials: S
authors.lastname: Grimmer
authors.forename: Elin
authors.initials: E
authors.lastname: Strand
authors.forename: Torgeir
authors.initials: T
authors.lastname: Flatmark
authors.forename: Anni
authors.initials: A
authors.lastname: Vedeler
mesh_terms: D020413:3' Untranslated Regions / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D017306:Annexin A2 / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D002417:Cattle; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011132:Polyribosomes / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D001613:beta 2-Microglobulin / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D017306:Annexin A2; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D001613:beta 2-Microglobulin