Standardization and quality control for the determination of alpha-glucosidase in seminal plasma.
We attempted to provide an instruction for the standardization of alpha-glucosidase level determination and quality controls within the same laboratory and among different laboratories. Each of 51 semen samples was divided into two aliquots, centrifuging at 1000 g for 10 min or 3000 g for 15 min. The alpha-glucosidase level and sperm concentration of each sample were assayed. The alpha-glucosidase level in seminal plasma obtained at 3000 g for 15 min centrifugation was significantly lower than that at 1000 g for 10 min (p = 0.001). An additional 6 samples of seminal plasma with or without phenylmethylsulfonyl fluoride (PMSF), obtained at 3000 g for 15 min centrifugation, were frozen for 20 days, and each of 6 samples was determined for their alpha-glucosidase levels after thawing every other day. There was no significant difference between alpha-glucosidase levels in seminal plasma regardless of the presence of PMSF. The alpha-glucosidase level increased with the length of abstinence period. In conclusion, centrifugal velocity and abstinence time should be standardized in the alpha-glucosidase determination. Frozen seminal plasma may serve as the sample for quality control among clinical laboratories.
Lu
J-C
JC
Department of Reproduction and Genetics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, PR China. jclu@jlonline.com
Xu
H-R
HR
Chen
F
F
Huang
Y-F
YF
Lu
N-Q
NQ
D002498:Centrifugation; D015925:Cryopreservation; D006801:Humans; D008297:Male; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011786:Quality Control; D012661:Semen / Q000201:enzymology*; D012662:Semen Preservation; D012746:Sexual Abstinence / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects; D013997:Time Factors; D000520:alpha-Glucosidases / Q000032:analysis*
D010664:Phenylmethylsulfonyl Fluoride; D000520:alpha-Glucosidases