title: Caffeic acid derivatives production by hairy root cultures of Echinacea purpurea.
abstract: Inoculation of leaf explants of Echinacea purpurea (Moench) with Agrobacterium rhizogenes induced hairy roots with the capacity to produce biologically active caffeic acid derivatives (CADs), especially cichoric acid. The kinetics of growth, the uptake of macronutrients, and the accumulation of CADs were investigated in heterotrophically cultured hairy roots for a 50 day period. A maximum of 12.2 g L(-1) dry biomass was achieved in MS nutrients supplemented with 30 g L(-1) sucrose on day 40. The mathematical relationship between hairy root growth and conductivity was established during the exponential phase in Erlenmeyer flasks. HPLC analyses of methanolic (0.1% phosphoric acid; 70:30, v/v) extracts from hairy roots revealed the presence of important CADs: cichoric acid (19.21 mg g(-1) dry biomass), caftaric acid (3.56 mg g(-1) dry biomass), and chlorogenic acid (0.93 mg g(-1) dry biomass). These results demonstrate that biotechnological production of CADs in hairy roots of E. purpurea is possible. Furthermore, these hairy root cultures offer, for the very first time, an excellent biological model to study the biosynthetic pathway of medicinally important CADs.
authors.affiliation: Phytochemical Engineering Group, National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, People's Republic of China. czliu@home.ipe.ac.cn
authors.forename: Chun-Zhao
authors.initials: CZ
authors.lastname: Liu
authors.forename: Bilal H
authors.initials: BH
authors.lastname: Abbasi
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Murch
authors.forename: Praveen K
authors.initials: PK
authors.lastname: Saxena
mesh_terms: D002082:Butylamines / Q000737:chemistry / Q000378:metabolism; D002109:Caffeic Acids / Q000737:chemistry* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D020900:Echinacea / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism
chemical_list: D002082:Butylamines; D002109:Caffeic Acids; C053521:isobutylamine; C040048:caffeic acid