[Experimental studies on hepatotoxicity of rats induced by Fructus Gardeniae].
OBJECTIVE
To study the hepatotoxicity effects in rats with different extract of Fructus Gardeniae.


METHODS
Observe the change of appearance, behavior and weight of rats through oral gavage daily for 3 d. Weigh the liver and calculate the liver index. Detect the ALT, AST and TBIL. Observe the liver tissue by optical microscope.


RESULTS
The weight and index of liver were increased by 3.08 g x kg(-1) aqueous extract, 1.62 g x kg(-1) alcoholic extract and 0.28 g x kg(-1) geniposide, compared to those of the blank group (P < 0.005, P < 0.001) and the activities of ALT, AST and the content of TBIL were also increased, compared to those of the blank group (P < 0.05, P < 0.001). The liver cells were obviously swell, necrotic and changed with inflammatory infiltrate.


CONCLUSIONS
Aqueous extract, alcoholic extract and geniposide displayed hepatotoxicity, and the geniposide which was the main substance of the Fructus Gardeniae might be mainly responsible for the hepatotoxicity.
Yang
Hong-jun
HJ
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing.
Fu
Mei-hong
MH
Wu
Zi-lun
ZL
Liang
Ri-xing
RX
Huang
Lu-qi
LQ
Fang
Jing
J
Li
Geng
G
Cao
Ying
Y
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000633:toxicity*; D005260:Female; D005638:Fruit / Q000737:chemistry; D032062:Gardenia / Q000737:chemistry; D039823:Iridoids / Q000302:isolation & purification / Q000633:toxicity*; D008099:Liver / Q000473:pathology*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D011714:Pyrans / Q000302:isolation & purification / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D039823:Iridoids; D011714:Pyrans; C007835:geniposide; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin