The lipid polyester composition of Arabidopsis thaliana and Brassica napus seeds.
Mature seeds of Arabidopsis thaliana and Brassica napus contain a complex mixture of aliphatic monomers derived from the non-extractable lipid polyesters deposited by various seed tissues. Methods of polyester depolymerization of solvent-extracted seeds and analysis of aliphatic monomers were compared. Sodium methoxide-catalyzed depolymerization, followed by GC analysis of the acetylated monomers, was developed for routine quantitative analysis suitable for 0.5g seed samples. In Arabidopsis seeds, the major C16 and C18 monomers identified included omega-hydroxy fatty acids and alpha,omega-dicarboxylic acids derived from palmitate, oleate and linoleate, and 9,10,18-trihydroxyoctadecenoic acid. Among monomers which can collectively be considered likely to be derived from suberin, docosan-1-ol, docosane-1,22-diol, 22-hydroxydocosanoic acid, 24-hydroxytetracosanoic acid, tetracosane-1,24-dioic acid and ferulic acid were the major species. Compared to Arabidopsis, Brassica seeds showed a roughly similar proportion of monomer classes, with the exception that alkan-1ols were 3-fold higher. Also, there were much less C24 aliphatic species and significant amounts of C14-C16 alkan-1ols, including iso- and anteiso-methyl branched compounds. Dissection and analysis of mature Brassica seeds showed that the trihydroxy C18:1 fatty acid was found mainly in the embryo, while ferulate, fatty alcohols and C22 and C24 species were specific to the seed coat plus endosperm.
Molina
Isabel
I
Department of Plant Biology, Michigan State University, Wilson Road, East Lansing, MI 48824-1312, USA.
Bonaventure
Gustavo
G
Ohlrogge
John
J
Pollard
Mike
M
D017360:Arabidopsis / Q000737:chemistry* / Q000378:metabolism; D029688:Brassica napus / Q000737:chemistry* / Q000378:metabolism; D008055:Lipids / Q000032:analysis*; D011091:Polyesters / Q000032:analysis* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism
D008055:Lipids; D011091:Polyesters