[A study on the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein].
OBJECTIVE
To study the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein.


METHODS
Four specific siRNAs were obtained by transcription in vitro. After transfection with the siRNAs into HepG2.2.15 cells for 3 days, the inhibitive effects of La protein were analyzed by Western blot; the content changes of HBsAg, HBeAg and HBV-DNA were detected by ECL and RT-PCR.


RESULTS
In comparison to normal cells, La protein was less in the cells. There was less La protein in the cells trans-infected with siRNAs. HBsAg, the HBeAg and HBV-DNA secreted by the cells transfected with siRNA were also less than that in the normal cells.


CONCLUSIONS
There is a correlation between La protein and HBV mRNA and the expression of HBV protein.
Zhang
Hui
H
Clinical Laboratory, Oriental Hospital, Shanghai 201200, China.
Sun
Jin-Hui
JH
Geng
Hong-Lian
HL
Fan
Lie-Ying
LY
Liu
Gao-Lin
GL
Tan
Long-Yi
LY
D001324:Autoantigens / Q000378:metabolism*; D045744:Cell Line, Tumor; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006515:Hepatitis B virus / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering; D012367:RNA, Viral; D012261:Ribonucleoproteins / Q000378:metabolism*; D000097585:SS-B Antigen
D001324:Autoantigens; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012367:RNA, Viral; D012261:Ribonucleoproteins; D000097585:SS-B Antigen