title: Post-transcriptional regulation of the xynA expression by a novel mRNA binding protein, XaiF.
abstract: XaiF, a novel 32kDa protein encoded by the ORF located in the immediate downstream of the xynA gene of Bacillus stearothermophilus No. 236, was identified to be the xylanase-specific trans-activator. In this study, the positive effect of XaiF was confirmed to be xylanase-specific, and the results from Northern blot and in vitro transcription assays showed that the XaiF increased the xynA transcripts at post-transcriptional step. Moreover, analysis of the mRNA decay rate led to the assertion that the XaiF functions to stabilize the xynA mRNA. Intriguingly, in vitro RNA-protein binding assay and analysis using gst-xynA 3'-UTR chimeric gene constructs demonstrated that the XaiF stabilizes xynA mRNA by direct binding onto the 3'-UTR of the mRNA.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Jeong
authors.forename: Eung-Ryoung
authors.initials: ER
authors.lastname: Lee
authors.forename: Cheol-Won
authors.initials: CW
authors.lastname: Yun
authors.forename: Ssang-Goo
authors.initials: SG
authors.lastname: Cho
authors.forename: Yong-Jin
authors.initials: YJ
authors.lastname: Choi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D001411:Geobacillus stearothermophilus / Q000502:physiology*; D039381:RNA Cap-Binding Proteins / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D039381:RNA Cap-Binding Proteins