[Oxidative stress in nitroglycerin tolerance and treatment with 3,4,5,6-tetrahydroxyxanthone].
OBJECTIVE
To determine the relationship between the nitroglycerin tolerance and the stimulation of radical oxygen species (ROS) production, and the therapeutical effect of 3,4,5,6-tetrahydroxyxanthone.


METHODS
Vasodilator responses to nitroglycerin were examined in the isolated thoracic aorta. The contents of ROS,and cGMP were determined in the cultured human umbilical vein endothelial cells.


RESULTS
3,4,5,6-tetrahydroxyxanthone could significantly reduce the inhibition of relaxation by nitroglycerin. 3,4,5,6-tetrahydroxyxanthone could significantly inhibit the ROS increase and increase the cGMP level.


CONCLUSIONS
Nitroglycerin tolerance is associated with the stimulation of ROS production,and the reversal of nitroglycerin tolerance with 3,4,5,6-tetrahydroxyxanthone is related to the reduction of ROS.
Shi
Rui-zheng
RZ
Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhang
Guo-gang
GG
Bai
Yong-ping
YP
Li
Yuan-jian
YJ
Hu
Gao-yun
GY
Chen
Jia
J
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001013:Aorta, Thoracic / Q000166:cytology; D002478:Cells, Cultured; D004361:Drug Tolerance; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008297:Male; D005996:Nitroglycerin / Q000494:pharmacology*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology; D044004:Xanthones / Q000494:pharmacology*
C518384:3,4,5,6-tetrahydroxyxanthone; D000975:Antioxidants; D017382:Reactive Oxygen Species; D044004:Xanthones; D005996:Nitroglycerin