title: Altered expression of alpha3-containing GABAA receptors in the neocortex of patients with focal epilepsy.
abstract: Impaired transmission in GABAergic circuits is thought to contribute to the pathogenesis of epilepsy. Although it is well established that major reorganization of GABA(A) receptor subtypes occurs in the hippocampus of patients with medically refractory temporal lobe epilepsy (TLE), it is unclear whether this disorder is also associated with alterations in GABA(A) receptor subtypes in the neocortex. Here we have investigated immunohistochemically the subunit composition and neocortical distribution of three major GABA(A) receptor subtypes using antibodies specifically recognizing the subunits alpha1, alpha2, alpha3, beta2/3 and gamma2. Cortical tissue was obtained at surgery from patients with TLE and hippocampal sclerosis (HS; n = 9), TLE associated with neocortical lesions (non-HS; n = 12) and frontal lobe epilepsy (FLE; n = 5), with post-mortem samples serving as controls (n = 4). A distinct laminar and neuronal expression pattern of the alpha-subunit variants was found across the neocortical regions examined in the temporal and frontal lobes in both control and patient tissue samples. In the five patients with FLE, GABA(A) receptor subunit staining was unchanged as compared to controls. In patients with TLE we observed a marked decrease in alpha3-subunit staining in the superficial neocortical layers (I-III), but no change in the deep layers (V and VI) or in the expression pattern of the alpha1 and alpha2-subunits. Reduced expression in alpha3-containing GABA(A) receptors was detected in six out of nine patients of the HS group and four out of twelve patients of the non-HS group. Histopathological changes were present in eight out of the ten patients with decreased alpha3-subunit staining. The selective reduction in alpha3-containing GABA(A) receptors was confirmed using semiquantitative measurements of optical density (OD). The specific changes unique to alpha3-subunit expression in the superficial neocortical layers of patients with TLE suggest that this subtype is of particular significance in the reorganization of cortical GABAergic systems in focal epilepsy.
authors.affiliation: Institute of Pharmacology and Toxicology, University of Zurich Zurich, Switzerland. loupf@pharma.unizh.ch
authors.forename: Fabienne
authors.initials: F
authors.lastname: Loup
authors.forename: Fabienne
authors.initials: F
authors.lastname: Picard
authors.forename: Véronique M
authors.initials: VM
authors.lastname: André
authors.forename: Pierre
authors.initials: P
authors.lastname: Kehrli
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yonekawa
authors.forename: Heinz-Gregor
authors.initials: HG
authors.lastname: Wieser
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Fritschy
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004828:Epilepsies, Partial / Q000378:metabolism* / Q000473:pathology; D017034:Epilepsy, Frontal Lobe / Q000378:metabolism / Q000473:pathology; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism / Q000473:pathology; D005260:Female; D006624:Hippocampus / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D019579:Neocortex / Q000737:chemistry* / Q000473:pathology; D009474:Neurons / Q000737:chemistry; D011963:Receptors, GABA-A / Q000032:analysis*; D012598:Sclerosis
chemical_list: D011963:Receptors, GABA-A