[Molecular approaches to the control of onchocerciasis in Mexico].
Onchocerciasis is one of the major causes of blindness in the World, with about 17.7 million infected, particularly in West Africa. In Mexico, onchocerciasis is also present and has been subjected to control since 1923. The standard diagnosis of onchocerciasis is by the detection of microfilariae by skin biopsy and transmission is evaluated by detection of Onchocerca volvulus larvae in the vector. Classically, this was carried out by manual dissection of Simuliumn ochraceun s.l. However, with the use of ivermectin, a drug that kills microfilariae but not the adult worms, the skin biopsy is becoming no longer useful for detecting microfilariae levels and due to the reduced transmission, fly dissection is no longer viable. The subject of this paper is to present the immunological and molecular techniques developed to supersede the skin biopsy and fly dissection, and their diagnostic ability to assess the impact of multiple bi-annual mass ivermectin treatments on O. volvulus transmission in Mexico.
Rodríguez-Pérez
Mario A
MA
Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, Tamaulipas. mrodriguez@ipn.mx
D000354:Africa, Western / Q000453:epidemiology; D000818:Animals; D001706:Biopsy; D015342:DNA Probes; D005369:Filaricides / Q000627:therapeutic use*; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D007300:Insect Control; D007303:Insect Vectors / Q000469:parasitology*; D007559:Ivermectin / Q000627:therapeutic use*; D007843:Latin America / Q000453:epidemiology; D008018:Life Cycle Stages; D008800:Mexico / Q000453:epidemiology; D008842:Microfilariae / Q000302:isolation & purification; D017181:Onchocerca volvulus / Q000187:drug effects / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification; D009855:Onchocerciasis / Q000175:diagnosis / Q000188:drug therapy / Q000453:epidemiology / Q000276:immunology / Q000517:prevention & control* / Q000635:transmission; D015827:Onchocerciasis, Ocular / Q000175:diagnosis / Q000469:parasitology; D016133:Polymerase Chain Reaction; D011159:Population Surveillance; D012843:Simuliidae / Q000469:parasitology*; D012867:Skin / Q000469:parasitology / Q000473:pathology
D015342:DNA Probes; D005369:Filaricides; D007559:Ivermectin