title: Detection of Genogroup I and II human picobirnaviruses showing small genomic RNA profile causing acute watery diarrhoea among children in Kolkata, India.
abstract: Picobirnaviruses (PBVs) with bisegmented small RNA genome profile (1.75 and 1.55kbp for segment 1 and 2, respectively) were detected from 1999 to 2003 in faecal specimens of acute watery diarrhoea cases, largely children (n=20) and an adult in Kolkata, India. Varying degrees of dehydration necessitated their visit to hospital for further treatment and management of acute watery diarrhoea. PBV was associated with rotavirus (n=3) or astrovirus (n=3) and with both in one case. No co-infection with norovirus, sapovirus or adenovirus was detected in the picobirnavirus positive cases. No co-infection with parasites (Cryptosporidium spp., Giardia spp., Entamoeba spp., helminths) or bacteria (Vibrio spp., Shigella spp., Escherichia coli) was detected among the picobirnavirus positive cases. There was a single instance of co-infection with Salmonella spp. (n=1). PBVs not associated with serious diarrhoea illness and showing large genome profile (2.3-2.6 and 1.5-1.9kbp for segment 1 and 2, respectively) have earlier been reported in adult individuals and recently among children from a slum community in Kolkata, India. The short genome profile PBVs associated with acute watery diarrhoea may be another emerging diarrhoeagenic virus in Kolkata, India. Molecular characterization using reported primers PicoB25-PicoB43 for Genogroup I and PicoB23-PicoB24 for Genogroup II in RT-PCR showed the presence of Genogroup I PBVs (n=6) and Genogroup II PBVs (n=5), while some could not be amplified (n=3) with these primers. Sequence analysis of Genogroup I amplicons indicated remarkable sequence heterogeneity. After more than a decade, four PBV positives of Genogroup II were detected during this study. Phylogenetic analysis showed varying degree of genetic diversity amongst PBV strains from Kolkata and other countries.
authors.affiliation: Division of Molecular Virology, Diarrhoeal Disease Research and Control Center, National Institute of Cholera & Enteric Diseases, P33 CIT Road, Scheme XM, Beliaghata, Kolkata (Calcutta) 700010, West Bengal, India.
authors.forename: Rittwika
authors.initials: R
authors.lastname: Bhattacharya
authors.forename: Ganesh C
authors.initials: GC
authors.lastname: Sahoo
authors.forename: Mukti K
authors.initials: MK
authors.lastname: Nayak
authors.forename: K
authors.initials: K
authors.lastname: Rajendran
authors.forename: P
authors.initials: P
authors.lastname: Dutta
authors.forename: Utpala
authors.initials: U
authors.lastname: Mitra
authors.forename: M K
authors.initials: MK
authors.lastname: Bhattacharya
authors.forename: T N
authors.initials: TN
authors.lastname: Naik
authors.forename: S K
authors.initials: SK
authors.lastname: Bhattacharya
authors.forename: Triveni
authors.initials: T
authors.lastname: Krishnan
mesh_terms: D000328:Adult; D000367:Age Factors; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000503:physiopathology / Q000821:virology*; D003968:Diarrhea, Infantile / Q000453:epidemiology / Q000503:physiopathology / Q000821:virology; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D018708:Picobirnavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015995:Prevalence; D012327:RNA Virus Infections / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000302:isolation & purification
chemical_list: D012367:RNA, Viral