Selective isolation of large chromosomal regions by transformation-associated recombination cloning for structural and functional analysis of mammalian genomes.
Transformation-associated recombination (TAR) cloning allows selective isolation of full-size genes and genomic loci as circular yeast artificial chromosomes in yeast. The method has a broad application for structural and functional genomics, long-range haplotyping, characterization of chromosomal rearrangements, and evolutionary studies. This chapter describes a basic protocol of gene isolation by TAR, as well as a method of conversion of TAR isolates into bacterial artificial chromosomes.
Kouprina
Natalay
N
Laboratory of Biosystems and Cancer, National Cancer Institute, NIH, Bethesda, MD, USA.
Noskov
Vladimir N
VN
Larionov
Vladimir
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D000818:Animals; D022201:Chromosomes, Artificial / Q000235:genetics*; D003001:Cloning, Molecular; D016678:Genome / Q000235:genetics / Q000502:physiology*; D008322:Mammals / Q000235:genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012641:Selection, Genetic; D014170:Transformation, Genetic