Construction of saccharide-modified DNAs by DNA polymerase.
Novel deoxyribonucleotide triphosphates bearing maltose or lactose groups were synthesized as substrates for DNA polymerase. The incorporation efficiencies of these modified substrates were investigated in both primer extension reactions and PCR. The stability and conformation of saccharide-modified dsDNAs were assessed by UV absorbance melting experiments and CD analysis. Enzymatic incorporation of saccharide-modified substrates can be used for the efficient production of saccharide-modified DNAs.
Matsui
Masayuki
M
Graduate School of Science and Technology, Kobe University, 3-11 Tsurukabuto, Kobe 657-8501, Japan.
Nishiyama
Yoshitaka
Y
Ueji
Shin-ichi
S
Ebara
Yasuhito
Y
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet; D014544:Uridine Triphosphate / Q000138:chemical synthesis / Q000737:chemistry
D002241:Carbohydrates; D017931:DNA Primers; D007202:Indicators and Reagents; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D014544:Uridine Triphosphate