title: Development of a dendritic cell vaccine against measles for patients following hematopoietic cell transplantation.
abstract: Most patients who have undergone hematopoietic cell transplantation (HCT) lose specific immunity to measles. However, due to its immunosuppressive potential, it has been recommended that a live attenuated measles vaccination be administered two years following HCT. Measles virus (MV) glycoproteins including hemagglutinin (HA) are expressed on MV-infected dendritic cells (DCs), and they impair efficient antigen presentation between the DC and T cell. We produced a DC-based vaccine against MV by loading DCs with MV-infected autologous DCs. MV in the infected DCs was inactivated using ultraviolet-B. The DC-based vaccine neither expressed HA nor inhibited allogeneic T cell proliferation, while it induced the production of interferon-gamma (IFN-gamma) by autologous CD4 and CD8 naive T cells ex vivo. Importantly, the vaccine derived from patients who had undergone HCT also efficiently induced IFN-gamma producing cells. These findings indicate that our DC-based MV vaccine induces MV-specific immunity even in post-HCT patients without causing immunosuppression.
authors.affiliation: Department of Pediatrics and Cell Transplantation, Mie University School of Medicine, Mie, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nashida
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kumamoto
authors.forename: Eiichi
authors.initials: E
authors.lastname: Azuma
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hirayama
authors.forename: Mariko
authors.initials: M
authors.lastname: Araki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamada
authors.forename: Francis
authors.initials: F
authors.lastname: Dida
authors.forename: Shotaro
authors.initials: S
authors.lastname: Iwamoto
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Tamaki
authors.forename: Masaru
authors.initials: M
authors.lastname: Ido
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Ihara
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Komada
mesh_terms: D000293:Adolescent; D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003713:Dendritic Cells / Q000166:cytology*; D006023:Glycoproteins / Q000737:chemistry; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects* / Q000379:methods*; D006801:Humans; D007165:Immunosuppression Therapy; D007371:Interferon-gamma / Q000378:metabolism; D008457:Measles / Q000517:prevention & control*; D008458:Measles Vaccine / Q000737:chemistry*; D008875:Middle Aged
chemical_list: D006023:Glycoproteins; D008458:Measles Vaccine; D007371:Interferon-gamma