Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk.
Specific immunoglobulin (IgY) from egg yolk against Aeromonas hydrophila was produced by immunization of White Leghorn hens with formalin-killed whole cells of A. hydrophila. ELISA test using A. hydrophila as the coating antigen revealed that the specific antibody titer started to increase in the egg yolk at the 13th day post-immunization (P/N=2.18), reached the peak at the 56th day (P/N=13.82), and remained at high level until day 133 (P/N=7.03). The antibody was purified by saturated ammonium sulphate with a recovery rate of 63.5%. The specific IgY inhibited the growth of A. hydrophila at a concentration of 1.0 mg/ml during the 18 h incubation. Pre-treatment of polyploid gibel carps Carassius auratus Gibelio with specific IgY had a protection rate of 60% (6/10) against challenge with A. hydrophila, while none of the fishes in the control groups receiving sterile phosphate buffered saline (PBS) or non-specific IgY survived the challenge. Treatment of fishes with the specific IgY 4 h after the challenge also had lower mortality (70%, 7/10), a 30% reduction against the control PBS or non-specific IgY groups (10/10). These results indicate that specific IgY antibodies could be obtained easily from hens immunized with an inactivated A. hydrophila and could provide a novel alternative approach to control of diseases in fishes caused by this organism.
Li
Xiao-liang
XL
Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China.
Shuai
Jiang-bing
JB
Fang
Wei-huan
WH
D016980:Aeromonas hydrophila / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D002645:Chickens / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004530:Egg Yolk / Q000737:chemistry*; D006054:Goldfish / Q000276:immunology* / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000517:prevention & control*; D007136:Immunoglobulins / Q000494:pharmacology / Q000627:therapeutic use*; D008826:Microbial Sensitivity Tests; D015996:Survival Rate; D013997:Time Factors
C016512:IgY; D007136:Immunoglobulins