[Effect of Rongshi granule on renal stone formation and osteopontin expression in rat urolithiasis model].
OBJECTIVE
To investigate the effect of Rongshi granule on osteopontin(OPN) expression.


METHODS
The urlisthiasis rats were induced by ethylene glycol (EG) and ammonium chloride, the control group rats were non-treated, and the Rongshi granule groups (low-dose group, middle-dose group and high-dose group) were administered Rongshi granule in addition to EG and ammonium chloride in 21 days. Pooled 24 h urine samples from each group were collected weekly with the use of metabolic cages, the concentration of uric calcium and oxalic acid were respectively measured by EDTA and photoelectric colorimetric method. Eight animals from each group were killed at 0, 7, 14, and 21 days, kidneys were histologic examinaed and immunohistochemical staining.


RESULTS
The expression of kidney osteopontin in model group was obviously higher than that of control group (P <0.01), and was up to the highest at 21 days with 1.4 times (0.281 3/0.201 8) of the control group. The expression of kidney osteopontin in all of the Rongshi granule groups were lower than those of model group (P < 0.05), with an obvious dose-dependent manner. The degree of the kidney calcium oxalate crystal of the rats in all the Rongshi granule groups was much lower than that of model group, and the uric calcium and oxalic acid were much lower than those of model group (P < 0.01).


CONCLUSIONS
The Rongshi granule could inhibit the expression of osteopontin in rat urolithiasis model.
Huang
Ping
P
Tong-De Hospital of Zhejiang Provinice, Hangzhou 310012, China. huangpwly@sina.com
Yang
Su-Wen
SW
Huang
Wei-Hua
WH
Kong
Fan-Zhi
FZ
Lou
Yi-Jia
YJ
D000643:Ammonium Chloride; D000818:Animals; D002118:Calcium / Q000652:urine; D002129:Calcium Oxalate / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D019855:Ethylene Glycol; D005260:Female; D007668:Kidney / Q000378:metabolism*; D007669:Kidney Calculi / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D053495:Osteopontin / Q000378:metabolism*; D019815:Oxalic Acid / Q000652:urine; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D000643:Ammonium Chloride; D053495:Osteopontin; D002129:Calcium Oxalate; D019815:Oxalic Acid; D019855:Ethylene Glycol; D002118:Calcium