Increased calcium deposits and decreased Ca2+-ATPase in right ventricular myocardium of ascitic broiler chickens.
Right ventricular hypertrophy and failure is an important step in the development of ascites syndrome (AS) in broiler chickens. Cytoplasmic calcium concentration is a major regulator of cardiac contractile function and various physiological processes in cardiac muscle cells. The purpose of this study was to measure the right ventricular pressure and investigate the precise ultrastructural location of Ca(2+) and Ca(2+)-ATPase in the right ventricular myocardium of chickens with AS induced by low ambient temperature. The results showed that the right ventricular diastolic pressure of ascitic broilers was significantly higher than that of control broilers (P < 0.01), and the maximum change ratio of right intraventricular pressure (RV +/- dp/dt(max)) of ascitic broilers was significantly lower than that of the controls (P < 0.01). Extensively increased calcium deposits were observed in the right ventricular myocardium of ascitic broilers, whereas in the age-matched control broilers, calcium deposits were much less. The Ca(2+)-ATPase reactive products were obviously found on the sarcoplasmic reticulum and mitochondrial membrane of the control right ventricular myocardium, but rarely observed in the ascitic broilers. The data suggest that in ascitic broilers there is the right ventricular diastolic dysfunction, in which the overload of intracellular calcium and the decreased Ca(2+)-ATPase activity might be the important factors.
Li
K
K
Department of Animal Physiopathology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.
Qiao
J
J
Zhao
L
L
Dong
S
S
Ou
D
D
Wang
J
J
Wang
H
H
Xu
T
T
D000818:Animals; D001201:Ascites / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism / Q000648:ultrastructure; D002645:Chickens; D006352:Heart Ventricles / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D017380:Hypertrophy, Right Ventricular / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D011201:Poultry Diseases / Q000378:metabolism / Q000473:pathology*
D000252:Calcium-Transporting ATPases; D002118:Calcium