Giardia duodenalis: analysis of secreted proteases upon trophozoite-epithelial cell interaction in vitro.
Protease secretion by Giardia duodenalis trophozoites upon interaction with epithelial cells and its association with the parasite adhesion was studied in co-cultures of parasites with IEC6 epithelial cell monolayers in the presence or absence of protease inhibitors. Proteolytic activity in supernatants from trophozoites was enhanced when they were co-cultured with IEC6 cells. This activity was strongly inhibited by pre-incubation of live trophozoites with E-64 and TPCK and a concomitant inhibition of parasite adhesion to IEC6 cells was observed. These data suggest that trophozoites secrete cysteine-type proteases that play a role in the adhesion of G. duodenalis to epithelial cells.
Rodríguez-Fuentes
Guadalupe Beatriz
GB
Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social-Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
Cedillo-Rivera
Roberto
R
Fonseca-Liñán
Rocío
R
Argüello-García
Raúl
R
Muñoz
Onofre
O
Ortega-Pierres
Guadalupe
G
Yépez-Mulia
Lilián
L
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D002460:Cell Line; D004847:Epithelial Cells / Q000201:enzymology*; D005872:Giardia / Q000166:cytology / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000187:drug effects / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology*
D011480:Protease Inhibitors; D010447:Peptide Hydrolases