[Effect of andrographolide on QS regulating virulence factors production in Pseudomonas aeruginosa].
OBJECTIVE
To investigate the effect of andrographolide on virulence factors production in Pseudomonas aeruginosa.


METHODS
Growth rate, pyocyanin, proteolytic activity and elastase activity were measured with or without the presence of andrographolide. The effect of andrographolide on pyocyanin production, proteolytic activity and elastase activity in PAO-JP2 was investigated simultaneously.


RESULTS
The andrographolide did not affect the growth of PAO1 in planktonic culture. The production of pyocyanin, proteolytic activity and elastase activity were significanthy suppressed in P. aeruginosa cultures grown in the presence of andrographolide. However, these effects were not observed in PAO-JP2.


CONCLUSIONS
The inhibiting effect of andrographolide on virulence factors production in P. aeruginosa may play a role in its anti-infection activity.
Li
Hong-tao
HT
Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China.
Qin
Hui-min
HM
Wang
Wei-hua
WH
Li
Guo-jun
GJ
Wu
Chun-ming
CM
Song
Jian-xin
JX
D035685:Andrographis / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D010196:Pancreatic Elastase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity; D011710:Pyocyanine / Q000378:metabolism; D037521:Virulence Factors / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D004224:Diterpenes; D037521:Virulence Factors; C030419:andrographolide; D011710:Pyocyanine; D010447:Peptide Hydrolases; D010196:Pancreatic Elastase