[Fingerprints of Rhizoma Coptidis from Shizhu by HPLC].
OBJECTIVE
To establish a sensitive and specific HPLC method for the quality control of Rhizoma Coptidis collected from shizhu in chongqing.


METHODS
The HPLC fingerprints of Rhizoma Coptidis from shizhu were obtained from Waters instrument. The methods was performed on a Diamonsil C18 column gradient eluted with acetonitrile-0.05 mol/L KH2PO4 (pH3 with H3PO4) at the flow rate of 0.8 m/min. The temperature of column was 25 degrees C and the UV detection wavelength was 270nm.


RESULTS
The HPLC fingerprint of Rhizoma Coptis, showing 16 characteristic peaks, was established from 10 lots of Rhizoma Coptis. By comparision of the retention time and the on-line UV spectra of chemical standards, peak 11, 12, 13, 14 and 15 were identified as Epiberberine, Jatrorrhizine, Coptisine, Palmatine and Berberine.


CONCLUSIONS
The HPLC fingerprint of Rhizoma Coptidis with high specificity can be used to control its quality.
Huang
Xiao-ping
XP
Chongqing Academy of Chinese Materia Medica, China.
Li
Long-yun
LY
Qu
Xian-you
XY
Cui
Guang-lin
GL
D001599:Berberine / Q000031:analogs & derivatives* / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D039323:Coptis / Q000737:chemistry* / Q000145:classification; D004355:Drug Stability; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry
D001600:Berberine Alkaloids; D001599:Berberine