title: Transactivation of the proximal promoter of human oxytocin gene by TR4 orphan receptor.
abstract: The human testicular receptor 4 (TR4) shares structural homology with members of the nuclear receptor superfamily. Some other members of this superfamily were able to regulate the transcriptional activity of the human oxytocin (OXT) promoter by binding to the first DR0 regulatory site. However, little investigation was conducted systematically in the study of the second dDR4 site of OXT proximal promoter, and the relationship between the first and the second sites of OXT promoter. Here, we demonstrated for the first time that TR4 could increase the proximal promoter activity of the human OXT gene via DR0, dDR4, and OXT (both DR0 and dDR4) elements, respectively. TR4 might induce OXT gene expression through the OXT element in a dose-dependent manner. However, there is no synergistic effect between DR0 and dDR4 elements during TR4 transactivation. Taken together, these results suggested that TR4 should be one of important regulators of OXT gene expression.
authors.affiliation: Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
authors.forename: Chih-Pin
authors.initials: CP
authors.lastname: Wang
authors.forename: Yi-Fen
authors.initials: YF
authors.lastname: Lee
authors.forename: Chawnshang
authors.initials: C
authors.lastname: Chang
authors.forename: Han-Jung
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010121:Oxytocin / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: C472782:NR2C2AP protein, human; D009687:Nuclear Proteins; D012097:Repressor Proteins; D010121:Oxytocin