Effect of sesamol on radiation-induced cytotoxicity in Swiss albino mice.
The radio-protective ability of sesamol (SM) at various doses viz., 0, 10, 25, 40, 50, 70 and 100mg/kg bw, administered intraperitoneally 30min prior to 9.5Gy whole-body gamma-irradiation was studied in Swiss albino mice. Radiation toxicity and mortality were observed during a period of 30 days and the percentage mortality was calculated. SM pretreatment with 50mg/kg bw was found to be the most effective dose in maintaining body weight and in reducing the percentage mortality, while 100mg/kg bw was found to be more effective in maintaining the spleen index and in stimulation of endogenous spleen colony-forming units. Pretreatment with SM (50mg/kg bw) in mice irradiated with 15Gy significantly reduced dead, inflammatory, mitotic and goblet cells in irradiated jejunum. SM at 50mg/kg bw also increased crypt cells, maintained villus height, and prevented mucosal erosion. Nuclear enlargement in epithelial cells was found less in SM-treated mice compared with the irradiated control. The radiation-induced decrease in endogenous antioxidant enzymes (GSH, GST, catalase) and the increase in lipid peroxidation were also reduced by pretreatment with SM [50 and 100mg/kg bw] at all monitored post-irradiation intervals. There was no protection at a dose less than 25mg/kg bw.
Parihar
Vipan Kumar
VK
Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal 576104, India.
Prabhakar
K R
KR
Veerapur
Veeresh P
VP
Kumar
M Sudheer
MS
Reddy
Y Rosi
YR
Joshi
Ravi
R
Unnikrishnan
M K
MK
Rao
C Mallikarjuna
CM
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D052117:Benzodioxoles; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D005978:Glutathione / Q000378:metabolism; D006410:Hematopoiesis / Q000187:drug effects / Q000528:radiation effects; D007583:Jejunum / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D008815:Mice, Inbred Strains; D010636:Phenols / Q000494:pharmacology*; D011833:Radiation Injuries, Experimental / Q000401:mortality / Q000473:pathology / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D013438:Sulfhydryl Compounds / Q000378:metabolism; D015996:Survival Rate; D013997:Time Factors; D014916:Whole-Body Irradiation / Q000379:methods
D000975:Antioxidants; D052117:Benzodioxoles; D010636:Phenols; D011837:Radiation-Protective Agents; D013438:Sulfhydryl Compounds; C025583:sesamol; D005978:Glutathione