Activity of Ginkgo biloba extract and quercetin on thrombomodulin expression and tissue-type plasminogen activator secretion by human umbilical vein endothelial cells.
OBJECTIVE
In order to investigate the pharmacological properties of Ginkgo biloba extract (GBE) on improving blood circulation, the regulating action of GBE and quercetin (a main flavonoid ingredient in GBE) on thrombomodulin (TM) expression and tissue-type plasminogen activator (t-PA) secretion was studied.


METHODS
Using flow cytometer and gel image system respectively, we evaluated the TM expression and the t-PA secretion by human umbilical vein endothelial cells (HUVECs) in vitro.


RESULTS
The increase of TM expression on HUVECs surface was induced by GBE rather than quercetin in a dose- and time-dependent manner. Both GBE and quercetin increased the t-PA release significantly.


CONCLUSIONS
The effect of GBE on improving blood circulation may be partly attributed to its promoting TM expression and t-PA secretion by endothelial cells, and quercetin participated in the effect of GBE on t-PA secretion. However, the action of GBE on increasing TM expression needs further study.
Lan
Wen-Jun
WJ
Key Laboratory for Biomedical Engineering of Ministry of Education of China, Zhejiang University (Yuquan Campus), Hangzhou 310027, Zhejiang, China. lanwenjun0522@hotmail.com
Zheng
Xiao-Xiang
XX
D001775:Blood Circulation / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005434:Flow Cytometry; D020441:Ginkgo biloba / Q000737:chemistry*; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology*; D018180:Thrombomodulin / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014471:Umbilical Veins / Q000166:cytology
D010936:Plant Extracts; D018180:Thrombomodulin; D011794:Quercetin; D010959:Tissue Plasminogen Activator