Complete genomic characterization and antigenic relatedness of genogroup III, genotype 2 bovine noroviruses.
Bovine enteric noroviruses form a genogroup, III, distinct from the 2 human norovirus genogroups, I and II. Two genogroup III genotypes were suggested by partial genomic analyses. In the present study, analysis of the full-length genome sequence of Bo/Newbury2/76/UK and the more contemporary Newbury2-like virus, Bo/Dumfries/1994/UK, showed that both were 7311 nucleotides in length and had three open reading frames (ORFs), amino acids motifs typical of noroviruses, and 95% or greater amino acid identities to each other in all regions of their genome. Apart from the ORF1 NTPase region, their ORF1 regions had less than 90% identity to the genogroup III genotype 1 Bo/Jena/80/DE virus, confirming two genogroup III genotypes. A close antigenic relationship was demonstrated by ELISA between the genotype 2 viruses, which will allow their serological diagnosis.
Oliver
S L
SL
Department of Pathology and Infectious Diseases, Royal Veterinary College, London, UK.
Asobayire
E
E
Charpilienne
A
A
Cohen
J
J
Bridger
J C
JC
D020121:5' Untranslated Regions; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D016679:Genome, Viral; D005838:Genotype; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D014771:Virion / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure
D020121:5' Untranslated Regions; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral