Improved efficiency of bovine cloning by autologous somatic cell nuclear transfer.
Somatic cell nuclear transfer (SCNT) has been used for the cloning of various mammals. However, the rates of successful, healthy birth are generally poor. To improve cloning efficiency, we report the utilization of an 'autologous SCNT' cloning technique in which the somatic nucleus of a female bovine donor is transferred to its own enucleated oocyte recovered by ovum pick up, in contrast to the routine 'allogeneic SCNT' procedure using oocytes from unrelated females. Our results showed that embryos derived from autologous SCNThave significantly higher developmental competence than those derived from allogeneic SCNT, especiallyat the eight-cell (60 vs 44%), morula (45 vs 36%), and blastocyst (38 vs 23%) stages. The pregnancy and birth rates were also higher for the autologous (39 and 23%), compared to the allogeneic (22 and 6%) SCNT groups. Genome-wide histone3-lysine9 methylation profiles reveal that autologous SCNTembryos have less epigenetic defects than the allogeneic SCNTembryos. This study indicates that autologous SCNT can improve the efficiency of bovine cloning with less reprogramming deficiency.
Yang
Xiao-Yu
XY
Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University, 24/1400 West Beijing Road, Shanghai 200040, People's Republic of China.
Li
Hua
H
Ma
Qing-Wen
QW
Yan
Jing-Bin
JB
Zhao
Jiang-Guo
JG
Li
Hua-Wei
HW
Shen
Hai-Qing
HQ
Liu
Hai-Feng
HF
Huang
Ying
Y
Huang
Shu-Zhen
SZ
Zeng
Yi-Tao
YT
Zeng
Fanyi
F
D000818:Animals; D002417:Cattle; D019976:Cloning, Organism / Q000379:methods*; D004247:DNA / Q000032:analysis; D019175:DNA Methylation; D046149:Embryo Culture Techniques; D047108:Embryonic Development; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D008297:Male; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D011256:Pregnancy Outcome; D017422:Sequence Analysis, DNA; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D004247:DNA