title: A new generation of sterile and radiopaque impression materials: an in vitro cytotoxicity study.
abstract: Impression materials are largely used to record the geometry of dental tissue. Hence, the assessment of their possible cytotoxicity is a necessary step in the evaluation of their biocompatibility. The present study is carried out to evaluate the cytotoxicity of a new elastomeric sterile and radiopaque impression material. Human gingival fibroblasts, cultured in vitro are exposed directly to Elite Implant in three different viscosities, heavy, medium, and light. At 3, 9, 24, 48, and 72 h, the cellular proliferation is evaluated. In parallel, human gingival fibroblasts are exposed indirectly by means of fluid extracts of Elite Implant. The cellular viability is evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, (MTT) assay (Sigma, St Louis, Mo). The gingival fibroblasts proliferation and viability are unaffected by the presence of Elite Implant. This new impression material may represent a safe medical device for clinical and surgical applications. In addition, this material is radiopaque and, thus, can be identified radiographically.
authors.affiliation: Department of Neurosciences, Section of Dentistry, University of Modena and Reggio Emilia, Policlinico, Largo del Pozzo 71, Modena, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Coppi
authors.forename: Chiara
authors.initials: C
authors.lastname: Paolinelli Devincenzi
authors.forename: Sergio
authors.initials: S
authors.lastname: Bortolini
authors.forename: Ugo
authors.initials: U
authors.lastname: Consolo
authors.forename: Roberta
authors.initials: R
authors.lastname: Tiozzo
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003287:Contrast Media / Q000633:toxicity*; D003760:Dental Impression Materials / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D011145:Polyvinyls / Q000633:toxicity*; D012833:Siloxanes / Q000633:toxicity*; D013242:Sterilization; D013778:Tetrazolium Salts; D013844:Thiazoles; D014783:Viscosity
chemical_list: D003287:Contrast Media; D003760:Dental Impression Materials; D011145:Polyvinyls; D012833:Siloxanes; D013778:Tetrazolium Salts; D013844:Thiazoles; C034183:vinyl polysiloxane; C022616:thiazolyl blue