title: Trichinella nativa and Trichinella T9 in the Hokkaido island, Japan.
abstract: Trichinella sp. muscle larvae were isolated from the thigh muscle of two red foxes (Vulpes vulpes) captured in Sapporo and Otofuke, Hokkaido, Japan, in 2003. Multiplex PCR designed for genotyping the genus Trichinella revealed that the Sapporo isolate showed a specific pattern to T. britovi complex (T. britovi, Trichinella T8 and Trichinella T9) and the Otofuke isolate showed that to T. nativa. Nucleotide sequences of a part of the mitochondrial cytochrome oxidase subunit I (COI) gene and internal transcribed spacer 2 (ITS2) of the Sapporo isolate showed the highest similarity to those of Trichinella T9, a species detected in the mainland of Japan. This study shows that both T. nativa and Trichinella T9 are circulating in wildlife of the Hokkaido island.
authors.affiliation: Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Kanai
authors.forename: Nariaki
authors.initials: N
authors.lastname: Nonaka
authors.forename: Ken
authors.initials: K
authors.lastname: Katakura
authors.forename: Yuzaburo
authors.initials: Y
authors.lastname: Oku
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005589:Foxes / Q000469:parasitology*; D005838:Genotype; D005849:Gerbillinae; D007564:Japan; D007814:Larva / Q000235:genetics; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000469:parasitology; D009132:Muscles / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014234:Trichinella / Q000235:genetics* / Q000302:isolation & purification; D014235:Trichinellosis / Q000469:parasitology / Q000662:veterinary*
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV