title: Fluorescence in situ hybridization (FISH) analysis of primary ocular adnexal MALT lymphoma.
abstract: BACKGROUND
  It remains unknown whether primary ocular adnexal extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) is a homogeneous entity, as there are few reports of the results of cytogenetic or molecular analyses of these tumors.
  
  
  METHODS
  We performed interphase fluorescence in situ hybridization (FISH) analysis to detect translocations and aneuploidy in 34 cases of primary ocular adnexal MALT lymphoma, and reviewed the histopathological findings. Correlations between the results of FISH analysis, the histopathological features and the clinical data were also analyzed.
  
  
  RESULTS
  Among the 34 cases, FISH analysis revealed t(14;18)(q32;q21) in one case, trisomy 3 in 21 cases (62%), and trisomy 18 in 16 cases (47%). The cases with trisomy 18 had significantly more prominent lymphoepithelial lesions (LELs) and less nodularity in the tumors. In regard to the clinical correlations, tumors with trisomy 18 were observed predominantly in females and younger patients; also, in the majority of the cases, the tumor was of conjunctival origin. All the cases with recurrence showed trisomy 18 in the tumor.
  
  
  CONCLUSIONS
  Primary ocular adnexal MALT lymphoma is a significantly heterogeneous entity. Cases with trisomy 18 may have unique clinicopathological features.
authors.affiliation: Hematology and Stem Cell Transplantation Division, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-ku, Tokyo, Japan. kaztanimo@qmed.hosp.go.jp
authors.forename: Kazuki
authors.initials: K
authors.lastname: Tanimoto
authors.forename: Naohiro
authors.initials: N
authors.lastname: Sekiguchi
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Yokota
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kaneko
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Akiko Miyagi
authors.initials: AM
authors.lastname: Maeshima
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Matsuno
authors.forename: Mine
authors.initials: M
authors.lastname: Harada
authors.forename: Kensei
authors.initials: K
authors.lastname: Tobinai
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kobayashi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005134:Eye Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018442:Lymphoma, B-Cell, Marginal Zone / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D014314:Trisomy / Q000235:genetics