A label-free protein microfluidic array for parallel immunoassays.
A label-free protein microfluidic array for immunoassays based on the combination of imaging ellipsometry and an integrated microfluidic system is presented. Proteins can be patterned homogeneously on substrate in array format by the microfluidic system simultaneously. After preparation, the protein array can be packed in the microfluidic system which is full of buffer so that proteins are not exposed to denaturing conditions. With simple microfluidic channel junction, the protein microfluidic array can be used in serial or parallel format to analyze single or multiple samples simultaneously. Imaging ellipsometry is used for the protein array reading with a label-free format. The biological and medical applications of the label-free protein microfluidic array are demonstrated by screening for antibody-antigen interactions, measuring the concentration of the protein solution and detecting five markers of hepatitis B.
Wang
Zhan-Hui
ZH
Institute of Mechanics, Chinese Academy of Sciences, Beijing, PR China.
Meng
Yong-Hong
YH
Ying
Pei-Qing
PQ
Qi
Cai
C
Jin
Gang
G
D000818:Animals; D006510:Hepatitis B Antibodies / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006513:Hepatitis B e Antigens / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*; D012709:Serum Albumin / Q000032:analysis
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D007074:Immunoglobulin G; D012709:Serum Albumin