title: [HPLC determination of acteoside in Radix Rehmanniae].
abstract: OBJECTIVE
  To develop an HPLC method for the determination of acteoside in Radix Rehmanniae.
  
  
  METHODS
  The chromatographic conditions were as follows: Polaris C18(4.6 mm x 250 mm, 5 microm) column, a mobile phase in gradient mode composed of acetonitrile 0.1% acetic acid solution, a flow rate of 1.0 mL x min(-1), and 334 nm as the detection wavelength.
  
  
  RESULTS
  Acteoside showed good linear relationship at the range of 10-500 microg x mL(-1) (r = 0.9990). The average recovery was 100.1%, RS D 3.7%.
  
  
  CONCLUSIONS
  The proposed method promised to be applicable for the quality control of Radix Rehmanniae.
authors.affiliation: Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Gui-xin
authors.initials: GX
authors.lastname: Chou
authors.forename: Zheng-tao
authors.initials: ZT
authors.lastname: Wang
authors.forename: Zhi-bi
authors.initials: ZB
authors.lastname: Hu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D032267:Rehmannia / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D059808:Polyphenols
chemical_list: D005960:Glucosides; D010636:Phenols; C058956:acteoside; D059808:Polyphenols