title: Emdogain-gel stimulates proliferation of odontoblasts and osteoblasts.
abstract: OBJECTIVE
  The purpose of this study was to determine whether a premixed form of enamel matrix derivative (EMD), Emdogain-gel, has the same property as the original formula of EMD in stimulating the proliferation of osteoblasts and odontoblasts.
  
  
  METHODS
  Osteoblast cell line (MC3T3) and odontoblast cell line (MDPC) were cultured in the 6-well culture plates and treated in 4 different groups: (1) culture medium control, (2) 100 microg/mL Emdogain-gel directly added to the culture medium, (3) culture medium with a culture plate insert, and (4) 100 microg/mL Emdogain-gel added onto a culture plate insert. The culture plate insert prevented direct contact between Emdogain-gel and the cells. After 3-day incubation, cell morphology was examined and the total cell number per well was counted. Data were analyzed using 1-way ANOVA.
  
  
  RESULTS
  Emdogain-gel significantly increased cell number of both osteoblasts and odontoblasts regardless the presence of the culture plate insert.
  
  
  CONCLUSIONS
  Emdogain-gel stimulates cell proliferation of odontoblasts and osteoblasts. The direct contact between Emdogain-gel and cells is not required. Heat treatment of EMD and premix with propylene glycol alginate did not change its property of releasing bioactive molecules for promoting cell proliferation.
authors.affiliation: Division of Endodontology, School of Dental Medicine, The University of Connecticut Health Center, Farmington, CT.
authors.forename: Jin
authors.initials: J
authors.lastname: Jiang
authors.forename: Golnaz
authors.initials: G
authors.lastname: Goodarzi
authors.forename: Jianing
authors.initials: J
authors.lastname: He
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.forename: Kamran E
authors.initials: KE
authors.lastname: Safavi
authors.forename: Larz S W
authors.initials: LS
authors.lastname: Spångberg
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D016475:3T3 Cells; D000704:Analysis of Variance; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D005782:Gels; D006801:Humans; D051379:Mice; D009804:Odontoblasts / Q000187:drug effects*; D010006:Osteoblasts / Q000187:drug effects*
chemical_list: D003470:Culture Media; D003746:Dental Enamel Proteins; D005782:Gels; C109069:enamel matrix proteins