title: Comparative study of the water oxidizing reactions and the millisecond delayed chlorophyll fluorescence in photosystem II at different pH.
abstract: Water splitting activity, the multiline EPR signal associated with S(2)-state of the CaMn(4)-cluster and the fast and slow phases of the induction curve of the millisecond delayed chlorophyll fluorescence from photosystem II (PSII) in the pH range of 4.5-8.5 were studied in the thylakoid membranes and purified PSII particles. It has been found that O(2) evolution and the multiline EPR signal were inhibited at acidic (pK approximately 5.3) and alkaline (pK approximately 8.1) pH values, and were maximal at pH 6.0-7.0. Our results indicate that the loss of O(2) evolution and the S(2)-state multiline EPR signal associated with the decrease of the millisecond delayed chlorophyll fluorescence only in alkaline region (pH 7.0-8.5). Possible correlations of the millisecond delayed chlorophyll fluorescence components with the donor side reactions in PSII are discussed.
authors.affiliation: Biophysics Laboratory, Institute of Botany, National Academy of Sciences, Patamdar Road 40, Baku AZ-1073, Azerbaijan. r.gasanov@box.az
authors.forename: Ralphreed
authors.initials: R
authors.lastname: Gasanov
authors.forename: Samira
authors.initials: S
authors.lastname: Aliyeva
authors.forename: Sachiko
authors.initials: S
authors.lastname: Arao
authors.forename: Aygun
authors.initials: A
authors.lastname: Ismailova
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Katsuta
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kitade
authors.forename: Shuji
authors.initials: S
authors.lastname: Yamada
authors.forename: Asako
authors.initials: A
authors.lastname: Kawamori
authors.forename: Fikret
authors.initials: F
authors.lastname: Mamedov
mesh_terms: D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D014867:Water
chemical_list: D045332:Photosystem II Protein Complex; D014867:Water; D002734:Chlorophyll; D010100:Oxygen