title: Comparison of the relative toxicity of travoprost 0.004% without benzalkonium chloride and latanoprost 0.005% in an immortalized human cornea epithelial cell culture system.
abstract: The purpose of this study was to compare the relative toxicity of a new topical ophthalmic glaucoma medication, travoprost 0.004% without benzalkonium chloride (BAK), with that of commercially available latanoprost 0.005% (preserved with 0.02% BAK) in immortalized human corneal epithelial cells (HCEs). Tissue culture plates (96 well) containing HCEs were divided into 6 groups. Two groups served as negative controls (70% methanol and gentamicin). Another 2 groups--1 in corneal epithelial culture media and the other in a hydroxypropyl (HP)-Guargellable lubricant eyedrop--served as live controls. The travoprost 0.004% without BAK and latanoprost 0.005% groups were exposed to 100 microL of the undiluted solutions. Cells were incubated for 25 min at 37 degree C. A live/dead assay was used to measure the effects of travoprost without BAK and of latanoprost on HCEs compared to 70% methanol and culture medium. Between the 2 glaucoma medications tested, travoprost 0.004% preserved without BAK showed significantly less toxicity on HCEs than did latanoprost 0.005%. This difference may have ramifications in terms of tolerability for patients who use these topical glaucoma drugs on a long-term basis.
authors.affiliation: Department of Ophthalmology Hermann Eye Center,University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Yee
authors.forename: Evan G
authors.initials: EG
authors.lastname: Norcom
authors.forename: Xinping C
authors.initials: XC
authors.lastname: Zhao
mesh_terms: D000959:Antihypertensive Agents / Q000633:toxicity*; D001548:Benzalkonium Compounds / Q000633:toxicity*; D002478:Cells, Cultured; D003008:Cloprostenol / Q000031:analogs & derivatives* / Q000633:toxicity; D006801:Humans; D066298:In Vitro Techniques; D000077338:Latanoprost; D014677:Pharmaceutical Vehicles; D011310:Preservatives, Pharmaceutical / Q000633:toxicity*; D011461:Prostaglandins F, Synthetic / Q000633:toxicity*; D000069557:Travoprost
chemical_list: D000959:Antihypertensive Agents; D001548:Benzalkonium Compounds; D014677:Pharmaceutical Vehicles; D011310:Preservatives, Pharmaceutical; D011461:Prostaglandins F, Synthetic; D003008:Cloprostenol; D000077338:Latanoprost; D000069557:Travoprost