Common and distinct gene expression patterns induced by the herbicides 2,4-dichlorophenoxyacetic acid, cinidon-ethyl and tribenuron-methyl in wheat.
In wheat, herbicides are used to control weeds. Little is known about the changes induced in the metabolism of tolerant plants after herbicide treatment. The impact of three herbicides [2,4-dichlorophenoxyacetic acid (2,4-D), cinidon-ethyl and tribenuron-methyl] on the wheat transcriptome was studied using cDNA microarrays. Gene expression of plants grown in a controlled environment or in the field was studied between 24 h and 2 weeks after treatment. Under controlled conditions, 2,4-D induced genes of the phenylpropanoid pathway soon after treatment. Cinidon-ethyl triggered peroxidase and defence-related gene expression under controlled conditions, probably because reactive oxygen species are released by photo-oxidation of protoporphyrin-IX. The same genes were upregulated in the field as under controlled conditions, albeit at a weaker level. These results show that cinidon-ethyl specifically induces genes involved in plant defence. Under controlled conditions, tribenuron-methyl did not change the expression profile immediately after treatment, but defence-related genes were upregulated after 1 week. Sulfonylurea compounds such as tribenuron-methyl specifically inhibit acetolactate synthase and are rapidly detoxified, but the activity of some of the resulting metabolites could explain later changes in gene expression. Finally, overexpression of the isopropylmalate synthase gene, involved in branched-chain amino acid synthesis, and of defence-related genes was observed in the field after sulfonylurea treatment.
Pasquer
Frédérique
F
Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland.
Ochsner
Urs
U
Zarn
Jürg
J
Keller
Beat
B
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D000179:Acrylates / Q000494:pharmacology*; D001190:Arylsulfonates / Q000494:pharmacology*; D004777:Environment; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006540:Herbicides / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism; D014908:Triticum / Q000187:drug effects*
D000179:Acrylates; D001190:Arylsulfonates; D006540:Herbicides; D007211:Indoles; C518218:ethyl 2-chloro-3-(2-chloro-5-(1,2-cyclohex-1-enedicarboximido)phenyl)acrylate; D015084:2,4-Dichlorophenoxyacetic Acid; C050296:metsulfuron methyl