Quantification of 3R,3'R-zeaxanthin in plant derived food by a diastereomeric dilution assay applying chiral high-performance liquid chromatography.
For quantification of 3R,3'R-zeaxanthin in plant derived food, a new diastereomeric dilution assay (DIDA) was developed using chiral high-performance liquid chromatography coupled to diode-array detection (HPLC/DAD). As diastereomeric internal standard (I.S.), 3R,3'S-(meso)-zeaxanthin was applied. DIDA showed sufficient sensitivity with low limits of detection and quantification (18 microg100 g(-1) and 45 microg 100 g(-1)), a good inter-assay coefficient of variation (2.4%), determined by analysis of a certified BCR reference material, and a good recovery (94.7%). To prove the applicability of DIDA, various plant materials were analyzed, showing that DIDA is highly advisable if saponification is applied during work-up to hydrolyze xanthophyll esters.
Schlatterer
Jörg
J
University of Hohenheim, Institute of Food Chemistry, Garbenstrasse 28, 70599 Stuttgart, Germany.
Maurer
Svenja
S
Breithaupt
Dietmar E
DE
D053000:Analytic Sample Preparation Methods; D002138:Calibration; D002212:Capsicum / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D005504:Food Analysis / Q000379:methods*; D031281:Hippophae / Q000737:chemistry; D010944:Plants / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018724:Spinacia oleracea / Q000737:chemistry; D013237:Stereoisomerism; D024341:Xanthophylls / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry; D065146:Zeaxanthins
D024341:Xanthophylls; D065146:Zeaxanthins; C584722:meso-zeaxanthin