Protective effect of resveratrol against oxidative damage of UVA irradiated HaCaT cells.
OBJECTIVE
To observe the photoprotective effect and possible mechanisms of resveratrol for ultraviolet A (UVA) irradiated HaCaT cells.


METHODS
HaCaT cells under UVA irradiation with 5J/cm(2) were interfered with 0.01 mmol/L and 0.1 mmol/L resveratrol. The testing objects were divided into a control and a UVA irradiation group, and then we detected the proliferation capacity with methylthiazdyl tetrazolium (MTT) and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, content of maleic dialdehyde (MDA) with hydroxylamine, colorimetric, thiobarbituric acid (TBA) methods. The ultrastructure was observed under electron microscope.


RESULTS
Resveratrol could enhance the proliferation activity, SOD, GSH-Px activity of HaCaT cells under UVA irradiation, decrease the content of MDA in dose-dependent manner (P<0.05). The electron microscope revealed that resveratrol could relieve the injury of HaCaT cells' ultrastructure.


CONCLUSIONS
Resveratrol can relieve the inhibition to HaCaT cell proliferation,injury of their ultrastructure and oxidation by UVA irradiation. The protection is dose-dependent. That resveratrol raises the oxidase activity and clears the oxyradical may account for these results.
Chen
Ming-liang
ML
Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410078, China.
Li
Ji
J
Xiao
Wei-rong
WR
Sun
Lei
L
Tang
Hua
H
Wang
Lin
L
Wu
Ling-yan
LY
Chen
Xiang
X
Xie
Hong-fu
HF
D002461:Cell Line, Transformed; D004249:DNA Damage / Q000528:radiation effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism*; D014466:Ultraviolet Rays
D011837:Radiation-Protective Agents; D013267:Stilbenes; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000077185:Resveratrol