title: From the genome to the proteome: uncovering peptides in the Apis brain.
abstract: Neuropeptides, critical brain peptides that modulate animal behavior by affecting the activity of almost every neuronal circuit, are inherently difficult to predict directly from a nascent genome sequence because of extensive posttranslational processing. The combination of bioinformatics and proteomics allows unprecedented neuropeptide discovery from an unannotated genome. Within the Apis mellifera genome, we have inferred more than 200 neuropeptides and have confirmed the sequences of 100 peptides. This study lays the groundwork for future molecular studies of Apis neuropeptides with the identification of 36 genes, 33 of which were previously unreported.
authors.affiliation: Department of Chemistry, University of Illinois, Urbana, IL 61801, USA.
authors.forename: Amanda B
authors.initials: AB
authors.lastname: Hummon
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Richmond
authors.forename: Peter
authors.initials: P
authors.lastname: Verleyen
authors.forename: Geert
authors.initials: G
authors.lastname: Baggerman
authors.forename: Jurgen
authors.initials: J
authors.lastname: Huybrechts
authors.forename: Michael A
authors.initials: MA
authors.lastname: Ewing
authors.forename: Evy
authors.initials: E
authors.lastname: Vierstraete
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Rodriguez-Zas
authors.forename: Liliane
authors.initials: L
authors.lastname: Schoofs
authors.forename: Gene E
authors.initials: GE
authors.lastname: Robinson
authors.forename: Jonathan V
authors.initials: JV
authors.lastname: Sweedler
mesh_terms: D000465:Algorithms; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees / Q000737:chemistry* / Q000235:genetics*; D001923:Brain Chemistry; D003062:Codon; D019295:Computational Biology; D017344:Genes, Insect; D049750:Genome, Insect; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry* / Q000235:genetics*; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics; D020543:Proteome
chemical_list: D003062:Codon; D019476:Insect Proteins; D009479:Neuropeptides; D011498:Protein Precursors; D020543:Proteome