title: Genomic stability and physiological assessments of live offspring sired by a bull clone, Starbuck II.
abstract: It appears that overt phenotypic abnormalities observed in some domestic animal clones are not transmitted to their progeny. The current study monitored Holstein heifers sired by a bull clone, Starbuck II, from weaning to puberty. Genomic stability was assessed by telomere length status and chromosomal analysis. Growth parameters, blood profiles, physical exams and reproductive parameters were assessed for 12 months (and compared to age-matched control heifers). Progeny sired by the clone bull did not differ (P>0.05) in weight, length and height compared to controls. However, progeny had lower heart rates (HR) (P=0.009), respiratory rates (RR) (P=0.007) and body temperature (P=0.03). Hematological profiles were within normal ranges and did not differ (P>0.05) between both groups. External and internal genitalia were normal and both groups reached puberty at expected ages. Progeny had two or three ovarian follicular waves per estrous cycle and serum progesterone concentrations were similar (P=0.99) to controls. Telomere lengths of sperm and blood cells from Starbuck II were not different (P>0.05) than those of non-cloned cattle; telomere lengths of progeny were not different (P>0.05) from age-matched controls. In addition, progeny had normal karyotypes in peripheral blood leukocytes compared to controls (89.1% versus 86.3% diploid, respectively). In summary, heifers sired by a bull clone had normal chromosomal stability, growth, physical, hematological and reproductive parameters, compared to normal heifers. Furthermore, they had moderate stress responses to routine handling and restraint.
authors.affiliation: Department of Population Medicine, Ontario Veterinary College, University of Guelph, Ontario, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Ortegon
authors.forename: D H
authors.initials: DH
authors.lastname: Betts
authors.forename: L
authors.initials: L
authors.lastname: Lin
authors.forename: G
authors.initials: G
authors.lastname: Coppola
authors.forename: S D
authors.initials: SD
authors.lastname: Perrault
authors.forename: P
authors.initials: P
authors.lastname: Blondin
authors.forename: W A
authors.initials: WA
authors.lastname: King
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D001774:Blood Chemical Analysis / Q000662:veterinary; D016022:Case-Control Studies; D002417:Cattle / Q000097:blood / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D019976:Cloning, Organism / Q000662:veterinary*; D005260:Female; D042822:Genomic Instability; D006304:Health Status; D008297:Male; D010641:Phenotype; D012098:Reproduction / Q000502:physiology*