Consistent dynamics suggests tight regulation of biophysical parameters in a small network of bursting neurons.
The neuronal firing patterns in the pyloric network of crustaceans are remarkably consistent among animals. Although this characteristic of the pyloric network is well-known, the biophysical mechanisms underlying the regulation of the systems output are receiving renewed attention. Computer simulations of the pyloric network recently demonstrated that consistent motor output can be achieved from neurons with disparate biophysical parameters among animals. Here we address this hypothesis by pharmacologically manipulating the pyloric network and analyzing the emerging voltage oscillations and firing patterns. Our results show that the pyloric network of the lobster stomatogastric ganglion maintains consistent and regular firing patterns even when entire populations of specific voltage-gated channels and synaptic receptors are blocked. The variations of temporal parameters used to characterize the burst patterns of the neurons as well as their intraburst spike dynamics do not display statistically significant increase after blocking the transient K-currents (with 4-aminopyridine), the glutamatergic inhibitory synapses (with picrotoxin), or the cholinergic synapses (with atropine) in pyloric networks from different animals. These data suggest that in this very compact circuit, the biophysical parameters are cell-specific and tightly regulated.
Szücs
Attila
A
Institute for Nonlinear Science, University of California, San Diego, California 92093-0402, USA. aszucs@ucsd.edu
Selverston
Allen I
AI
D015761:4-Aminopyridine / Q000494:pharmacology; D000200:Action Potentials / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D000818:Animals; D001703:Biophysics / Q000379:methods*; D004558:Electric Stimulation / Q000379:methods; D009415:Nerve Net / Q000166:cytology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D017711:Nonlinear Dynamics; D033521:Palinuridae; D026902:Potassium Channel Blockers / Q000494:pharmacology
D026902:Potassium Channel Blockers; D015761:4-Aminopyridine