Efficient isolation of anthraquinone-derivatives from Trichoderma harzianum ETS 323.
Anthraquinone-derivatives, chrysophanol and pachybasin, were purified by a silica column chromatography with two different solvent systems from Trichoderma harzianum ETS 323. The fungus was incubated in sugarcane bagasse solid medium at room temperature without rotation. Structure of chrysophanol was solved by X-ray diffraction and pachybasin by NMR spectra. About 233+/-13 mg of pure chrysophanol and 773+/-40 mg of pure pachybasin were recovered per kg of solid cultural medium, with yields 1.7+/-0.2% and 5.6+/-0.5%, respectively.
Liu
Shu-Ying
SY
Department of Molecular Biotechnology, Da-Yeh University, Changhua 51591, Taiwan.
Lo
Chaur-Tsuen
CT
Chen
Chinpiao
C
Liu
Ming-Yih
MY
Chen
Jun-Hui
JH
Peng
Kou-Cheng
KC
D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D018360:Crystallography, X-Ray; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D031786:Saccharum / Q000382:microbiology; D012822:Silicon Dioxide; D014242:Trichoderma / Q000737:chemistry* / Q000302:isolation & purification
D000880:Anthraquinones; D000935:Antifungal Agents; D012822:Silicon Dioxide; C027113:chrysophanic acid