[Optimization of the protocol for constructing transgenic plants of the white cabbage brassica oleracea var. capitata].
The strain Agrobacterium tumefaciens GV3101, which contains the pBar vector carrying the phosphinothricin acetyltransferase gene (bar) under the control of the 35SCMoV promoter and NOS 3' terminator, was used for genetic transformation of four white cabbage lines, Ges-3, Drv-2, Zmu 7, and Meg 2. The effect of different concentrations and combinations of phytohormones was studied, which allowed for choosing the cultivation conditions that provided a 63-78% regeneration efficiency. It was demonstrated that concerted action by natural and synthetic cytokinins is necessary for the lines studied. Overall, 26 transgenic plants were obtained using the optimized protocol for agrobacterial transformation. The transgenic nature of these plants was confirmed by PCR and dot-blot hybridization.
Gribova
T N
TN
Kamionskaia
A M
AM
Skriabin
K G
KG
D000123:Acetyltransferases / Q000096:biosynthesis / Q000235:genetics*; D016960:Agrobacterium tumefaciens / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001937:Brassica / Q000201:enzymology / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D000123:Acetyltransferases; C056243:phosphinothricin N-acetyltransferase