title: Dietary whey protein hydrolysate suppresses development of atopic dermatitis-like skin lesions induced by mite antigen in NC/Nga mice.
abstract: BACKGROUND
  Oral administration of enzymatic hydrolysate of cow's milk whey protein (WPH) has been reported to produce an anti-inflammatory effect. Since inflammation plays a role in dermatitis of allergic disease, we examined the influence of WPH on the development of atopic dermatitis (AD)-like skin lesions, induced in NC/Nga mice by the mite antigen Dermatophagoides pteronyssinus (Dp).
  
  
  METHODS
  AD-like skin lesions were induced on the pinnae and backs of NC/Nga mice by daily application of Dp for 4 weeks. Mice were fed cow's milk casein (control), WPH or casein protein hydrolysate (CPH) diets for 2 weeks prior to Dp application. Clinical skin conditions were evaluated periodically by a clinical severity score, total serum IgE and soluble E-selectin levels were measured by enzyme linked immunosorbent assay (ELISA).
  
  
  RESULTS
  WPH-fed mice showed significantly less AD-like skin lesions than those fed casein diets at 2 and 4 weeks after Dp application. In contrast, CPH-fed mice had manifestations in a similar manner as casein-fed mice did, and did not show an inhibitory effect. Serum soluble E-selectin levels, known as a marker of disease activity in AD patients, were significantly lower in the WPH diet group.
  
  
  CONCLUSIONS
  Our results suggest that in addition to its hypoallergenicity an anti-inflammatory function, dietary WPH might be useful for reducing the severity of AD-like skin lesions.
authors.affiliation: Food Science Institute, Division of Research and Development, Meiji Dairies Corporation, Kanagawa, Japan. NOBUKO_SHIMIZU@MEIJI-MILK.COM
authors.forename: Nobuko
authors.initials: N
authors.lastname: Shimizu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Dairiki
authors.forename: Saori
authors.initials: S
authors.lastname: Ogawa
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kaneko
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D040002:Dermatophagoides pteronyssinus / Q000276:immunology*; D005260:Female; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D051379:Mice; D008894:Milk Proteins / Q000008:administration & dosage*; D011492:Protein Hydrolysates / Q000494:pharmacology*; D000067816:Whey Proteins
chemical_list: D000941:Antigens; D007166:Immunosuppressive Agents; D008894:Milk Proteins; D011492:Protein Hydrolysates; D000067816:Whey Proteins