A novel human gene ZNF415 with five isoforms inhibits AP-1- and p53-mediated transcriptional activity.
The zinc finger proteins are the single largest class of transcription factors in human genome. Previous studies revealed that zinc finger proteins are involved in transcriptional activation and regulation of apoptosis, etc. Alternative splicing emerges as a major mechanism of generating protein diversity and many zinc finger proteins reported have isoforms. In this article, we identify and characterize five isoforms of a novel zinc finger gene named ZNF415; these five isoforms were named ZNF415-1 to ZNF415-5. The five isoforms display different subcellular localization and are expressed at different levels in both embryonic and adult tissues. Furthermore, the splicing variants of ZNF415 display different transcriptional activity. Except for ZNF415-1, overexpression of the other ZNF415 isoforms in COS-7 cells inhibits the transcriptional activities of AP-1 and p53, suggesting that the ZNF415 protein may be involved in AP-1- and p53-mediated transcriptional activity.
Cheng
Yingduan
Y
The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, Changsha, 410081 Hunan, PR China.
Wang
Yuequn
Y
Li
Yanmei
Y
Deng
Yun
Y
Hu
Junjian
J
Mo
Xiaoyang
X
Li
Ning
N
Li
Yongqing
Y
Luo
Na
N
Yuan
Wuzhou
W
Xiao
Jing
J
Zhu
Chuanbing
C
Wu
Xiushan
X
Liu
Mingyao
M
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D006801:Humans; D020033:Protein Isoforms / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers / Q000502:physiology*
D020033:Protein Isoforms; D012097:Repressor Proteins; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; C515602:ZNF415 protein, human