A novel highly boron tolerant bacterium, Bacillus boroniphilus sp. nov., isolated from soil, that requires boron for its growth.
Three strains of gram-positive, motile, rod-shaped and boron (B)-tolerant bacterium were isolated from naturally B containing soil of Hisarcik area in the Kutahya Province, Turkey. The strains, designated as T-14A, T-15Z(T) and T-17s, produced spherical or ellipsoidal endospores in a terminal bulging sporangium. The strains required B for the growth and can tolerate more than 450 mM B. These also tolerated up to 7.0% (w/v) NaCl in the presence of 50 mM B in agar medium but grew optimally without NaCl. The temperature range for growth was 16-37 degrees C (optimal of 30 degrees C), whereas the pH range was 6.5-9.0 (optimal of 7.5-8.5). The DNA G + C content was 41.1-42.2 mol% and the predominant cellular fatty acid was iso-C(15:0). The major respiratory quinone system was detected as MK-7 and the diamino acid of the peptidoglycan was meso-diaminopimelic acid. Based on phenotypic and chemotaxonomic characteristics, phylogenetic analysis of 16S rRNA gene sequences data and DNA-DNA re-association values, we concluded that the three strains belong to a novel species of the genus Bacillus, the type strain of which is T-15Z(T) and for which we proposed the name, B. boroniphilus sp. nov. (DSM 17376(T) = IAM 15287(T) = ATCC BAA-1204(T)).
Ahmed
Iftikhar
I
Biotechnology Research Centre, University of Tokyo, Yayoi 1-1-1, Bunkyo-Ku, Tokyo, 113-8657, Japan. atorufu@mail.ecc.u-tokyo.ac.jp
Yokota
Akira
A
Fujiwara
Toru
T
D001407:Bacillus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure; D001482:Base Composition / Q000235:genetics; D001895:Boron / Q000378:metabolism / Q000494:pharmacology*; D003960:Diaminopimelic Acid / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D004579:Electron Transport / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics; D019751:Intramolecular Transferases / Q000235:genetics; D008855:Microscopy, Electron, Scanning; D010457:Peptidoglycan / Q000378:metabolism; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism / Q000494:pharmacology*; D014421:Turkey
D029968:Escherichia coli Proteins; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012989:Soil Pollutants; D003960:Diaminopimelic Acid; D019751:Intramolecular Transferases; C094732:16S RNA pseudouridine 516 synthase, E coli; D001895:Boron