title: [Extraction, isolation and structure identification of polysaccharide in root of Salvia miltiorrhiza].
abstract: OBJECTIVE
  To study the polysaccharide of Salvia miltiorrhiza.
  
  
  METHODS
  The root was extracted by water and purified preliminarily by alcohol precipitation. The obtained crude polysaccharide purified by ion exchange resin DEAE-Sepharose Fast Flow then bleached with H2O2 and dialysed. Two portions of light yellow powder were obtained through lyophilization and named as SMP 1, SMP 0.5. On the basis of 13C-NMR, DEPT, IR spectra, monosaccharide composite analysis and part monosaccharide composite analysis, their structures were identified.
  
  
  RESULTS
  The molecular weight of SMP 1, SMP 0.5 was estimated to be 1.39 x 10(6), 4.03 x 10(5), respectively, and the structure of SMP 1 was mainly alpha-(1-->6) D-Glc linked a few alpha-(1-->2) D-Glc, SMP 0.5 was mainly a alpha-(1-->6) D-Glucosan.
  
  
  CONCLUSIONS
  SMP 1 and SMP0.5 were neutral homosaccharides that extracted from S. miltiorrhiza for the first time.
authors.affiliation: Department of Pharmacy, Zhejiang College of Traditional Chinese Medicine, Hangzhou. wanghongxj@yahoo.com
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Shun-chun
authors.initials: SC
authors.lastname: Wang
authors.forename: Zheng-tao
authors.initials: ZT
authors.lastname: Wang
authors.forename: Jian-fang
authors.initials: JF
authors.lastname: Shen
mesh_terms: D008970:Molecular Weight; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D011134:Polysaccharides