Distribution of calbindin-D28k, neuronal nitric oxide synthase, and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) in the lateral nucleus of the sheep amygdaloid complex.
This study describes calbindin-D28k (CB), neuronal nitric oxide synthase (nNOS), and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) expression in the lateral nucleus of the sheep amygdaloid complex. Double immunofluorescence protocol was used in order to determine whether there is colocalization of CB and nNOS. The CB-immunoreactive (IR) neuronal population was composed especially of non-pyramidal neurons, but a few pyramidal cells were also present. The non-pyramidal neurons showed a multipolar and, occasionally, a fusiform morphology. The comparison between single-labeled CB-IR non-pyramidal neurons and cells belonging to CB-IR neuronal population showed they were identical for morphology, mean size, and distribution. The single-labeled CB-IR non-pyramidal neurons were only the 17.8% of the total non-pyramidal neurons counted. The nNOS-IR neuronal population was represented by non-pyramidal multipolar and fusiform neurons. Single-labeled nNOS-IR non-pyramidal neurons had the same morphology, mean area, and distribution as cells belonging to nNOS-IR neuronal population. Single-labeled nNOS-IR non-pyramidal neurons were more numerous than single-labeled CB-IR, and represented the 73.7% of total non-pyramidal neurons counted. NADPH-d-positive cells had the same morphology and distribution as the nNOS-IR neurons. Double immunolabeling (CB/nNOS) was found mostly in non-pyramidal multipolar neurons and only in a few non-pyramidal fusiform cells. These neurons had a mean perikaryal area significantly higher and significantly smaller than that of single-labeled nNOS and single-labeled CB-IR non-pyramidal neurons, respectively. CB and nNOS coexist only in a minority of non-pyramidal neurons (8.5%). The 32.4% of all CB-IR non-pyramidal neurons were nNOS-positive; only 10.4% of nNOS-IR non-pyramidal neurons were CB-positive. These results indicate that CB and nNOS are expressed by selective neurons and that the majority of nNOS-IR non-pyramidal neurons are lacking in CB.
Bombardi
Cristiano
C
Department of Veterinary Morphophysiology and Animal Productions, Faculty of Veterinary Medicine, University of Bologna, Via Tolara di Sopra, 50, 40064, Ozzano dell'Emilia (BO), Italy. cbombardi@vet.unibo.it
Grandis
Annamaria
A
Chiocchetti
Roberto
R
Lucchi
Maria Luisa
ML
D000679:Amygdala / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D064026:Calbindins; D005260:Female; D007150:Immunohistochemistry; D008954:Models, Biological; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D019581:Neuropil / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*; D013702:Temporal Lobe / Q000378:metabolism; D014018:Tissue Distribution
D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D052248:Nitric Oxide Synthase Type I; D009252:NADPH Dehydrogenase