title: Cutting edge: Deficiency of macrophage migration inhibitory factor impairs murine airway allergic responses.
abstract: Increased levels of macrophage migration inhibitory factor (MIF) in serum, sputum, and bronchioalveolar lavage fluid (BALF) from asthmatic patients and time/dose-dependent expression of MIF in eosinophils in response to phorbol myristate acetate suggest the participation of MIF in airway inflammation. In this study, we examined inflammation in OVA-sensitized mouse lungs in wild-type and MIF-deficient mice (MIF(-/-)). We report increased MIF in the lung and BALF of sensitized wild-type mice. MIF(-/-) mice demonstrated significant reductions in serum IgE and alveolar inflammatory cell recruitment. Reduced Th1/Th2 cytokines and chemokines also were detected in serum or BALF from MIF(-/-) mice. Importantly, alveolar macrophages and mast cells, but not dendritic cells or splenocytes, from MIF(-/-) mice demonstrated impaired CD4+ T cell activation, and the reconstitution of wild-type mast cells in MIF(-/-) mice restored the phenotype of OVA-induced airway inflammation, revealing a novel and essential role of mast cell-derived MIF in experimentally induced airway allergic diseases.
authors.affiliation: Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, 77th Avenue Louis Pasteur, Boston, MA 02115, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Xiaozhu
authors.initials: X
authors.lastname: Huang
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Wolters
authors.forename: Jiusong
authors.initials: J
authors.lastname: Sun
authors.forename: Shiro
authors.initials: S
authors.lastname: Kitamoto
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Richard
authors.initials: R
authors.lastname: Riese
authors.forename: Lin
authors.initials: L
authors.lastname: Leng
authors.forename: Harold A
authors.initials: HA
authors.lastname: Chapman
authors.forename: Patricia W
authors.initials: PW
authors.lastname: Finn
authors.forename: John R
authors.initials: JR
authors.lastname: David
authors.forename: Richard
authors.initials: R
authors.lastname: Bucala
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Shi
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D007073:Immunoglobulin E / Q000097:blood; D008168:Lung / Q000737:chemistry / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation / Q000235:genetics; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis / Q000172:deficiency* / Q000235:genetics; D016676:Macrophages, Alveolar / Q000276:immunology; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008817:Mice, Mutant Strains; D010047:Ovalbumin / Q000276:immunology; D011014:Pneumonia / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D018925:Chemokines; D016207:Cytokines; D008263:Macrophage Migration-Inhibitory Factors; D007073:Immunoglobulin E; D010047:Ovalbumin