title: In vitro activity of fluoroquinolones, vancomycin, and gentamicin against methicillin-resistant Staphylococcus aureus ocular isolates.
abstract: OBJECTIVE
  To determine the antibacterial activity of fluoroquinolones, vancomycin, and gentamicin against methicillin-resistant Staphylococcus aureus (MRSA) ocular surface isolates.
  
  
  METHODS
  Retrospective review.
  
  
  METHODS
  MRSA isolates were obtained from 21 patients. The MIC(50) (mean inhibitory concentration)(microg/ml) values of 31 MRSA ocular surface isolates were determined for gatifloxacin, moxifloxacin, ciprofloxacin, ofloxacin, vancomycin, and gentamicin using the Etest (AB Biodisk, Solna, Sweden) or the VITEK system (bioMérieux, Inc, Durham, North Carolina, USA). Susceptibility data were interpreted based on criteria specified by the Clinical and Laboratory Standards Institute (CLSI).
  
  
  METHODS
  MIC(50) values in microg/ml and interpretation of susceptibility or resistance.
  
  
  RESULTS
  In vitro resistance rates and median MIC(50) in microg/ml for the MRSA isolates were: gatifloxacin (71%, 8.0), moxifloxacin (68%, 8.0), ciprofloxacin (94%, 8.0), ofloxacin (94%, 8.0), vancomycin (0%, 1.0), and gentamicin (3%, 0.5).
  
  
  CONCLUSIONS
  MRSA ocular isolates exhibited a relatively high rate of in vitro resistance to all fluoroquinolones tested, including the fourth generation. In contrast, MRSA isolates were found to be highly sensitive to vancomycin and gentamicin.
authors.affiliation: Long Island Jewish Medical Center, Albert Einstein College of Medicine, New Hyde Park, New York, USA. Kotlus@gmail.com
authors.forename: Brett S
authors.initials: BS
authors.lastname: Kotlus
authors.forename: Richard A
authors.initials: RA
authors.lastname: Wymbs
authors.forename: Ernestine M
authors.initials: EM
authors.lastname: Vellozzi
authors.forename: Ira J
authors.initials: IJ
authors.lastname: Udell
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024841:Fluoroquinolones / Q000494:pharmacology*; D005839:Gentamicins / Q000494:pharmacology*; D006801:Humans; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D012189:Retrospective Studies; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D014640:Vancomycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D005839:Gentamicins; D014640:Vancomycin; D008712:Methicillin