title: Vector-based RNAi approaches for stable, inducible and genome-wide screens.
abstract: RNA interference (RNAi) has revolutionized the study of biology and offers numerous applications in basic biology as well as in drug discovery research. Since the discovery of RNAi, several tools have been developed to enable loss-of-function studies in mammalian systems. The efficacy of RNAi is dependent on specific and versatile RNAi triggers that have evolved to enable transient, stable and in-vivo applications. Recently developed genome-wide short hairpin RNA (shRNA) and microRNA-adapted short hairpin RNA (shRNAmir) libraries incorporate advances in shRNA design and molecular 'barcodes' to enable more complex RNAi screens and the opportunity to progress to more complex genetics in whole animals.
authors.affiliation: Open Biosystems Inc. 6705 Odyssey Drive, Huntsville, Al 35806, USA. gfewell@openbiosystems.com
authors.forename: Gwen D
authors.initials: GD
authors.lastname: Fewell
authors.forename: Karin
authors.initials: K
authors.lastname: Schmitt
mesh_terms: D000818:Animals; D004257:DNA Polymerase II / Q000235:genetics; D030561:Databases, Nucleic Acid; D004317:Doxorubicin / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005821:Genetic Techniques / Q000639:trends*; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D035683:MicroRNAs; D034741:RNA, Small Interfering; D012313:RNA; D004317:Doxorubicin; D004257:DNA Polymerase II