A mouse model of sensorimotor controlled cortical impact: characterization using longitudinal magnetic resonance imaging, behavioral assessments and histology.
The present study establishes a new mouse model for traumatic brain injury (TBI), using an electromechanically driven linear motor impactor device to deliver a lateral controlled cortical impact (CCI) injury to the sensorimotor cortex. Lesion cavity size was measured, and inter-animal consistency demonstrated, at 14 days post injury. Qualitative information regarding damage progression over time was obtained by scanning with high field magnetic resonance imaging (MRI) at five time points following injury. Functional impairment and recovery were measured with the Rotarod, gridwalk and cylinder tests, and lesion cavity volume was measured post mortem with thionin-stained tissue sections. The study establishes the reliability of a linear-motor based device for producing repeatable damage in a CCI model, demonstrates the power of longitudinal MRI in studying damage evolution, and confirms that a simple battery of functional tests record sensorimotor impairment and recovery.
Onyszchuk
Gregory
G
Hoglund Brain Imaging Center, University of Kansas Medical Center, 3901 Rainbow Boulevard, MS1052, Kansas City, KS 66160, USA.
Al-Hafez
Baraa
B
He
Yong-Yue
YY
Bilgen
Mehmet
M
Berman
Nancy E J
NE
Brooks
William M
WM
D000818:Animals; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology*; D002540:Cerebral Cortex / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology*; D003714:Denervation / Q000295:instrumentation / Q000379:methods; D004185:Disability Evaluation; D004195:Disease Models, Animal; D018450:Disease Progression; D004582:Electronics, Medical / Q000295:instrumentation / Q000379:methods*; D008137:Longitudinal Studies; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009044:Motor Cortex / Q000293:injuries / Q000473:pathology / Q000503:physiopathology; D009069:Movement Disorders / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D009410:Nerve Degeneration / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D011237:Predictive Value of Tests; D020127:Recovery of Function / Q000502:physiology