Expression of the fusion glycoprotein of Newcastle disease virus in transgenic rice and its immunogenicity in mice.
Transgenic plant has become an attractive bioreactor to produce high-value medical peptides and proteins in biomedical research. In present study, two expression cassettes, pUNDVF and pGNDVF containing the fusion protein gene of Newcastle disease virus (NDV F) under the control of maize ubiquitin (Ubi) promoter or rice glutelin (Gt1) promoter, respectively, were constructed, and introduced into rice (Oryzy sativa L.) by Agrobacterium-mediated transformation. A total of 12 independent transgenic rice lines were regenerated, and the result from PCR analysis indicated that the T-DNA region containing the NDV F chimeric gene had been integrated into the genome of transgenic rice plants. ELISA and Western-blot analyses revealed that the NDV F protein could be expressed and accumulated in both leaf and seed tissue of several transgenic rice plants. Moreover, the immunogenicity of expressed proteins was tested in a mouse model and the results showed that specific antibodies were elicited in mice immunized intraperitoneally with crude protein extracts from transgenic rice plants. It implied the potential of using transgenic rice-based expression systems as supplementary bioreactor for NDV engineering subunit vaccine.
Yang
Zhen-Quan
ZQ
College of Bioscience and Biotechnology and Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, 12 East Wenhui Road, Yangzhou, Jiangsu 225009, PR China.
Liu
Qiao-Quan
QQ
Pan
Zhi-Ming
ZM
Yu
Hen-Xiu
HX
Jiao
Xin-An
XA
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015870:Gene Expression; D051379:Mice; D008807:Mice, Inbred BALB C; D009522:Newcastle disease virus / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D014760:Viral Fusion Proteins; D014765:Viral Vaccines