Characterization of human cytotoxic T lymphocyte-associated antigen 4-immunoglobulin (hCTLA4Ig) expressed in transgenic rice cell suspension cultures.
The avidity for CD80Ig/CD86Ig and the in vitro immunosuppressive effect of recombinant human cytotoxic T lymphocyte-associated antigen 4-immunoglobulin, produced by transgenic rice cell suspension cultures (hCTLA4Ig(P)) with CHO-derived recombinant hCTLA4Ig (hCTLA4Ig(M)), were measured. Surface plasmon resonance (SPR) was used for kinetic binding analysis: hCTLA4Ig(P) and hCTLA4Ig(M) had higher avidity for CD80Ig/CD86Ig than for CD28Ig, and the avidity for CD80Ig/CD86Ig was similar. hCTLA4Ig(P) and hCTLA4Ig(M) had similar in vitro immunosuppressive activity against the expression of T cell-derived cytokines, such as IL-2, IL-4, and IFN-gamma, but did not suppress the expression of macrophage-derived cytokines, including TNF-alpha and IL-1beta, as well as NO. Thus the immunosuppressive mechanism of hCTLA4Ig(P) is also T cell-specific and it could therefore be used as an immunosuppressive agent with an equivalent potency to that of hCTLA4Ig(M).
Jung
Hahn-Sun
HS
Boryung Central Research Institute, Boryung Pharmaceutical Co. Ltd., Ansan, Kyungki-do, 425-120, Korea.
Koo
Jae-Kyung
JK
Lee
Song-Jae
SJ
Park
Cheon-Ik
CI
Shin
Ju-Yeop
JY
Kim
Myung-Hwan
MH
Tan
Hyun Kwang
HK
Lim
Sang Min
SM
Kim
Dong-Il
DI
D000069594:Abatacept; D000818:Animals; D000915:Antibody Affinity; D016466:CHO Cells; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D005240:Feasibility Studies; D018506:Gene Expression Regulation, Plant; D006801:Humans; D018796:Immunoconjugates / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D046549:Plantibodies / Q000235:genetics; D030821:Plants, Genetically Modified; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D018796:Immunoconjugates; D046549:Plantibodies; D011994:Recombinant Proteins; D009569:Nitric Oxide; D000069594:Abatacept