Diastereomeric and enantiomeric high-performance liquid chromatographic separation of synthetic anisodamine.
In order to investigate the enantiomeric pharmacokinetics and biotransformation of synthetic anisodamine (654-2), a cholinoceptor antagonist widely used in clinic in China, it has been preparatively separated into two racemates (I and II) by using ZORBAX Eclipse XDB-C18 column. The diastereo- and/or enantioseparations of 654-2, I and II were carried out by HPLC using CHIRALPAK AD-H as chiral stationary phase (CSP) and acetonitrile-2-propanol-DEA 97:3:0.1 (v/v/v) as mobile phase. The methods were optimized by studying mobile phase modifiers, concentration of modifier and column temperature. The HPLC method for the simultaneous separation of two pairs of enantiomers of 654-2 has been validated.
Yang
Li-Min
LM
Department of Pharmacy, Shanghai Jiao Tong University School of Medicine, South Chongqing Road 280, Shanghai 200025, China.
Xie
Yi-Fan
YF
Chen
Hong-Zhuan
HZ
Lu
Yang
Y
D000433:1-Propanol / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D010276:Parasympatholytics / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012991:Solanaceous Alkaloids / Q000302:isolation & purification*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D007202:Indicators and Reagents; D010276:Parasympatholytics; D012991:Solanaceous Alkaloids; D012997:Solvents; C003922:anisodamine; D000433:1-Propanol