title: [Effect of andrographolide on QS regulating virulence factors production in Pseudomonas aeruginosa].
abstract: OBJECTIVE
  To investigate the effect of andrographolide on virulence factors production in Pseudomonas aeruginosa.
  
  
  METHODS
  Growth rate, pyocyanin, proteolytic activity and elastase activity were measured with or without the presence of andrographolide. The effect of andrographolide on pyocyanin production, proteolytic activity and elastase activity in PAO-JP2 was investigated simultaneously.
  
  
  RESULTS
  The andrographolide did not affect the growth of PAO1 in planktonic culture. The production of pyocyanin, proteolytic activity and elastase activity were significanthy suppressed in P. aeruginosa cultures grown in the presence of andrographolide. However, these effects were not observed in PAO-JP2.
  
  
  CONCLUSIONS
  The inhibiting effect of andrographolide on virulence factors production in P. aeruginosa may play a role in its anti-infection activity.
authors.affiliation: Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Li
authors.forename: Hui-min
authors.initials: HM
authors.lastname: Qin
authors.forename: Wei-hua
authors.initials: WH
authors.lastname: Wang
authors.forename: Guo-jun
authors.initials: GJ
authors.lastname: Li
authors.forename: Chun-ming
authors.initials: CM
authors.lastname: Wu
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Song
mesh_terms: D035685:Andrographis / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D010196:Pancreatic Elastase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity; D011710:Pyocyanine / Q000378:metabolism; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004224:Diterpenes; D037521:Virulence Factors; C030419:andrographolide; D011710:Pyocyanine; D010447:Peptide Hydrolases; D010196:Pancreatic Elastase