Binding and inhibition assays for Siglecs.
Protein-carbohydrate interactions are believed to be important in many biological processes involving cellular communication. Although many of the experimental approaches for studying protein-carbohydrate interactions are similar to those commonly employed in protein-protein interactions, several important aspects have to be taken into account in binding assays with lectin-like receptors such as Siglecs, sialic acid-recognizing immunoglobulin-like lectins. This chapter describes experimental approaches using solid-phase assays with sialylated targets and complexed Siglec Fc-chimeras for binding studies or competitive inhibition assays. Such assays are useful to investigate various aspects of Siglec interactions with their carbohydrate-binding partners, such as the specificity and the molecular basis of sialic acid recognition.
Bock
Nadine
N
Centre for Biomolecular Interactions Bremen, University of Bremen, Bremen, Germany.
Kelm
Sørge
S
D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D012794:Sialic Acids / Q000378:metabolism*
D006023:Glycoproteins; D037102:Lectins; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D012794:Sialic Acids