title: Systemic distribution and elimination of plain and with Cy3.5 functionalized poly(vinyl alcohol) coated superparamagnetic maghemite nanoparticles after intraarticular injection in sheep in vivo.
abstract: PVA coated and fluorescent dye (Cy3.5) functionalized vinyl alcohol/vinyl amine copolymer coated superparamagnetic iron oxide nanoparticles (SPION) were evaluated for systemic distribution and elimination after intraarticular injection in sheep. Observation was done at 3, 24, 72, and 120 hours after injection using light microscopy, fluorescent microscopy, and confocal microscopy. No pathologic influence of SPION on the tissue harvested could be seen. A significantly increased iron content could be identified in the kidneys, lymph nodes, and spleen after injection of SPION. No particles were detected in the liver, the urinary, and the gall bladder. No positive fluorescent signal could be attributed to SPION throughout the organs. Our results indicated that the iron component of the SPION is possible to be incorporated into the physiologic iron metabolism after reabsorption in the proximal tubule system of the kidney and that concentration levels of Cy3.5 are too low to be detected throughout the body.
authors.affiliation: Musculoskeletal Research Unit, Equine Hospital, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hellstern
authors.forename: Katja
authors.initials: K
authors.lastname: Schulze
authors.forename: Bernhard
authors.initials: B
authors.lastname: Schöpf
authors.forename: Alke
authors.initials: A
authors.lastname: Petri-Fink
authors.forename: Benedikt
authors.initials: B
authors.lastname: Steitz
authors.forename: Sarah
authors.initials: S
authors.lastname: Kamau
authors.forename: Monika
authors.initials: M
authors.lastname: Hilbe
authors.forename: Sabine
authors.initials: S
authors.lastname: Koch-Schneidemann
authors.forename: Lloyd
authors.initials: L
authors.lastname: Vaughan
authors.forename: Michael
authors.initials: M
authors.lastname: Hottiger
authors.forename: Margarethe
authors.initials: M
authors.lastname: Hofmann
authors.forename: Heinrich
authors.initials: H
authors.lastname: Hofmann
authors.forename: Brigitte
authors.initials: B
authors.lastname: von Rechenberg
mesh_terms: D000818:Animals; D002232:Carbocyanines; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000493:pharmacokinetics; D052203:Ferrosoferric Oxide / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007270:Injections, Intra-Articular; D008657:Metabolic Clearance Rate; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009928:Organ Specificity; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D012756:Sheep; D014018:Tissue Distribution
chemical_list: D002232:Carbocyanines; D020099:Coated Materials, Biocompatible; C089187:cyanine dye 3; D011142:Polyvinyl Alcohol; D052203:Ferrosoferric Oxide