Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates from nonanoic acid by Pseudomonas putida KT2440.
Pseudomonas putida KT2440 grew on glucose at a specific rate of 0.48 h(-1) but accumulated almost no poly-3-hydroxyalkanoates (PHA). Subsequent nitrogen limitation on nonanoic acid resulted in the accumulation of only 27% medium-chain-length PHA (MCL-PHA). In contrast, exponential nonanoic acid-limited growth (mu = 0.15 h(-1)) produced 70 g l(-1) biomass containing 75% PHA. At a higher exponential feed rate (mu = 0.25 h(-1)), the overall productivity was increased but less biomass (56 g l(-1)) was produced due to higher oxygen demand, and the biomass contained less PHA (67%). It was concluded that carbon-limited exponential feeding of nonanoic acid or related substrates to cultures of P. putida KT2440 is a simple and highly effective method of producing MCL-PHA. Nitrogen limitation is unnecessary.
Sun
Zhiyong
Z
Department of Chemical Engineering, Queen's University, Kingston, ON, K7L 3N6, Canada.
Ramsay
Juliana A
JA
Guay
Martin
M
Ramsay
Bruce A
BA
D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D016958:Pseudomonas putida / Q000254:growth & development* / Q000378:metabolism
D005227:Fatty Acids; D011091:Polyesters; D002244:Carbon; C008776:pelargonic acid; D005947:Glucose; D009584:Nitrogen