Influence of pirfenidone on airway hyperresponsiveness and inflammation in a Brown-Norway rat model of asthma.
Pirfenidone was administered to sensitized Brown Norway rats prior to a series of ovalbumin challenges. Airway hyperresponsiveness, inflammatory cell infiltration, mucin and collagen content, and the degree of epithelium and smooth muscle staining for TGF-beta were examined in control, sensitized, and sensitized/challenged rats fed a normal diet or pirfenidone diet. Pirfenidone had no effect on airway hyperresponsiveness, but reduced distal bronchiolar cell infiltration and proximal and distal mucin content. Statistical analysis showed that the control group and sensitized/challenged pirfenidone diet group TGF-beta staining intensity scores were not significantly different from isotype controls, but that the staining intensity scores for the sensitized/challenged normal diet group was significantly different from isotype controls. These results suggest that pirfenidone treatment is effective in reducing some of the components of acute inflammation induced by allergen challenge.
Mansoor
Jim K
JK
Department of Physical Therapy, School of Pharmacy and Health Sciences, University of the Pacific, Stockton, CA, USA.
Decile
Kendra C
KC
Giri
Shri N
SN
Pinkerton
Kent E
KE
Walby
William F
WF
Bratt
Jennifer M
JM
Grewal
Harinder
H
Margolin
Solomon B
SB
Schelegle
Edward S
ES
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001249:Asthma / Q000188:drug therapy*; D001980:Bronchi / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D016535:Bronchial Hyperreactivity / Q000188:drug therapy*; D001985:Bronchial Provocation Tests; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D007249:Inflammation / Q000188:drug therapy*; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D009077:Mucins / Q000378:metabolism; D009130:Muscle, Smooth / Q000187:drug effects / Q000473:pathology; D010047:Ovalbumin; D011728:Pyridones / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D011914:Rats, Inbred BN; D020545:Respiratory Mucosa / Q000187:drug effects / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D009077:Mucins; D011728:Pyridones; D016212:Transforming Growth Factor beta; D010047:Ovalbumin; D003094:Collagen; C093844:pirfenidone