title: Laser microfabrication of hydroxyapatite-osteoblast-like cell composites.
abstract: We have developed a novel approach for layer-by-layer growth of tissue-engineered materials using a direct writing process known as matrix assisted pulsed laser evaporation direct write (MAPLE DW). Unlike conventional cell-seeding methods, this technique provides the possibility for cell-material integration prior to artificial tissue fabrication. This process also provides greater flexibility in selection and processing of scaffold materials. In addition, MAPLE DW offers rapid computer-controlled deposition of mesoscopic voxels at high spatial resolutions. We have examined MAPLE DW processing of zirconia and hydroxyapatite scaffold materials that can provide a medical device with nearly inert and bioactive implant-tissue interfaces, respectively. We have also demonstrated codeposition of hydroxyapatite, MG 63 osteoblast-like cells, and extracellular matrix using MAPLE DW. We have shown that osteoblast-like cells remain viable and retain the capacity for proliferation when codeposited with bioceramic scaffold materials. Our results on MG 63-hydroxyapatite composites can be extended to develop other integrated cell-scaffold structures for medical and dental applications.
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
authors.forename: A
authors.initials: A
authors.lastname: Doraiswamy
authors.forename: R J
authors.initials: RJ
authors.lastname: Narayan
authors.forename: M L
authors.initials: ML
authors.lastname: Harris
authors.forename: S B
authors.initials: SB
authors.lastname: Qadri
authors.forename: R
authors.initials: R
authors.lastname: Modi
authors.forename: D B
authors.initials: DB
authors.lastname: Chrisey
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D003188:Composite Resins; D005109:Extracellular Matrix; D006801:Humans; D006882:Hydroxyapatites / Q000627:therapeutic use*; D007834:Lasers; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D023822:Tissue Engineering / Q000379:methods*; D015040:Zirconium
chemical_list: D003188:Composite Resins; D006882:Hydroxyapatites; D015040:Zirconium; C028541:zirconium oxide