Development of a NACE method for simultaneous measurement of three adenosine monophosphate isomers in biomimicking prebiotic synthesis without sample pretreatment.
A practical NACE method was developed for simultaneous determination of three adenosine monophosphate (AMP) isomers. Separation of three AMP isomers was achieved using 200 mM Tris/H(3)BO(3) in acetontrile/water (2:1 v/v) at pH* 10.0 as the running buffer and +25 kV as the applied voltage over a bare fused-silica capillary of 50 microm id x 375 microm od x 54.5 cm (46 cm to the detector window). At 260 nm, the calibration curves were linear in the range of 1-100 microg/mL. The detection limits were less than 0.70 microg/mL. The recovery ranged from 94.5 to 106.4%. The intraday RSDs of the migration times were between 2.1 and 3.0%. The developed NACE method has been successfully applied for the determination of three AMP isomers in the real samples of biomimicking prebiotic synthesis reaction between N-(O,O-diisopropyl) phosphoryl amino acid and adenosine.
Song
Juanmei
J
Chemistry Department, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou, P. R. China.
Liu
Hongxia
H
Han
Po
P
Zhao
Canfang
C
Wu
Yangjie
Y
Zhang
Shusheng
S
D000249:Adenosine Monophosphate / Q000737:chemistry* / Q000302:isolation & purification*; D000596:Amino Acids / Q000737:chemistry*; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D009943:Organophosphorus Compounds / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000596:Amino Acids; D002021:Buffers; C517179:N-(O,O-diisopropyl) phosphoryl amino acid; D009943:Organophosphorus Compounds; D000249:Adenosine Monophosphate