title: Designer T cells by T cell receptor replacement.
abstract: T cell receptor (TCR) gene transfer is a convenient method to produce antigen-specific T cells for adoptive therapy. However, the expression of two TCR in T cells could impair their function or cause unwanted effects by mixed TCR heterodimers. With five different TCR and four different T cells, either mouse or human, we show that some TCR are strong--in terms of cell surface expression--and replace weak TCR on the cell surface, resulting in exchange of antigen specificity. Two strong TCR are co-expressed. A mouse TCR replaces human TCR on human T cells. Even though it is still poorly understood why some TCRalpha/beta combinations are preferentially expressed on T cells, our data suggest that, in the future, designer T cells with exclusive tumor reactivity can be generated by T cell engineering.
authors.affiliation: Max-Delbrück-Center for Molecular Medicine, Berlin, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Sommermeyer
authors.forename: Julia
authors.initials: J
authors.lastname: Neudorfer
authors.forename: Monika
authors.initials: M
authors.lastname: Weinhold
authors.forename: Matthias
authors.initials: M
authors.lastname: Leisegang
authors.forename: Boris
authors.initials: B
authors.lastname: Engels
authors.forename: Elfriede
authors.initials: E
authors.lastname: Noessner
authors.forename: Mirjam H M
authors.initials: MH
authors.lastname: Heemskerk
authors.forename: Jehad
authors.initials: J
authors.lastname: Charo
authors.forename: Dolores J
authors.initials: DJ
authors.lastname: Schendel
authors.forename: Thomas
authors.initials: T
authors.lastname: Blankenstein
authors.forename: Helga
authors.initials: H
authors.lastname: Bernhard
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Uckert
mesh_terms: D000818:Animals; D002460:Cell Line; D018014:Gene Transfer Techniques; D006801:Humans; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D023822:Tissue Engineering
chemical_list: D011948:Receptors, Antigen, T-Cell