[Genetic regulatory pathway of gene related breast cancer metastasis: primary study by linear differential model and k-means clustering].
OBJECTIVE
To investigate the potential genetic regulatory pathway of gene related breast cancer metastasis.


METHODS
Microarray technique was used to identify the gene expression profile and to screen the differential expression genes in breast cancer with special emphasis on the metastasis factors. A gene chip was available, obtained from the surgical samples, including breast cancer primary tissues and metastasis tissues, of 30 female patients with breast cancer at different clinical stages. Then potential genetic regulatory pathway of gene related breast cancer metastasis was analyzed with a linear differential model and k-means clustering.


RESULTS
Twenty-seven differential expression genes were identified. It was suggested that the potential regulatory pathway of gene related to breast cancer metastasis is made up of GRP, BPAG1, and SFRP2 genes.


CONCLUSIONS
The metastasis of breast cancer is related to the cancerization caused by the abnormal expression of multiple genes. It is reliable to analyze the Genetic regulatory pathway of gene related breast cancer metastasis by using multiple bio-informatic measures.
Sun
Bao-cun
BC
Cancer Institute and Cancer Hospital, Tianjin Medical University, Tianjin 300060, China.
Ni
Chun-sheng
CS
Feng
Yu-mei
YM
Li
Xiao-qing
XQ
Shen
Shi-yi
SY
Dong
Liu-huan
LH
Yuan
Yuan
Y
Zhang
Liang
L
Hao
Xi-shan
XS
D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D006801:Humans; D016014:Linear Models; D008207:Lymphatic Metastasis / Q000235:genetics*