In vitro selection of DNA aptamer against abrin toxin and aptamer-based abrin direct detection.
Abrin toxin as the target protein, belongs to class II ribosome-inactivating proteins family, has high toxicity to eukaryotic cells. Here, we firstly report the DNA aptamers, isolated by in vitro selection, recognize abrin toxin with high affinity and specificity, and have the advantage of no cross-reaction with structure-similar protein ricin toxin over antibodies. Then, a highly selective and sensitive aptamer-based abrin assay was established using a molecular light switching reagent [Ru(phen)(2)(dppz)](2+) with a limit of detection of 1 nM and a wide linear range from 1 to 400 nM with the correlation coefficient of 0.993. This assay can be successfully directly performed not only in physiological buffer but also in more complicated biological matrix, such as diluted serum.
Tang
Jijun
J
Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
Yu
Tao
T
Guo
Lei
L
Xie
Jianwei
J
Shao
Ningsheng
N
He
Zhike
Z
D000036:Abrin / Q000032:analysis* / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000032:analysis / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods*; D011485:Protein Binding; D052156:SELEX Aptamer Technique / Q000379:methods*
D052157:Aptamers, Nucleotide; D004268:DNA-Binding Proteins; D000036:Abrin