Ethanol decreases rat hepatic arylsulfatase A activity levels.
BACKGROUND
Arylsulfatase A (ASA) is an enzyme that catalyzes the degradation of sulfatides, a glycosphingolipid found in many tissues, but predominantly in myelin and kidney. Arylsulfatase A is 1 member of a family of sulfatases that is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. This conversion requires a novel oxygenase mechanism that can be inhibited by reactive oxygen species. Ethanol is known to cause an increase in reactive oxygen species in the liver. Because of its effect on the levels of hepatic reactive oxygen species, we hypothesized that ethanol would cause a specific decrease of rat hepatic ASA activity levels.


METHODS
Male Sprague-Dawley rats received ethanol-containing, Lieber-DeCarli liquid diets for 15 days, and control rats were pair-fed a liquid diet in which dextrose was isocalorically substituted for ethanol.


RESULTS
Arylsulfatase A activity levels decreased in livers of animals receiving alcohol compared with control animals. No significant changes in ASA activity levels were observed in the cerebral cortex and kidney. Furthermore, ethanol did not have any significant effect on hexosaminidase activity in any of the tissues examined.


CONCLUSIONS
Ethanol caused a tissue-specific decrease in hepatic ASA activity levels, but not hexosaminidase activity levels.
Jean
Smith
S
Department of Psychiatry, University of Medicine and Dentistry of New Jersey (UMDNJ)-Robert Wood Johnson Medical School, 671 Hoes Lane, Piscataway, NJ 08854, USA.
Kasinathan
Chinnaswamy
C
Buyske
Steven
S
Manowitz
Paul
P
D000818:Animals; D015153:Blotting, Western; D002492:Central Nervous System Depressants / Q000097:blood / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D002553:Cerebroside-Sulfatase / Q000378:metabolism*; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000097:blood / Q000494:pharmacology*; D006596:Hexosaminidases / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
D002492:Central Nervous System Depressants; D017382:Reactive Oxygen Species; D000431:Ethanol; D002553:Cerebroside-Sulfatase; D006596:Hexosaminidases