Antibody responses against HIV in rhesus macaques following combinations of mucosal and systemic immunizations with chimeric alphavirus-based replicon particles.
Mucosal and systemic transmission of HIV is prevalent. Therefore, mucosal followed by parenteral immunizations with chimeric vs. complete alphavirus-based replicon particles, encoding an HIV envelope glycoprotein, were tested. Female rhesus macaques were immunized intranasally and then intramuscularly. Following the immunizations, enhanced mucosal and systemic antibody responses were detected with the chimeric compared to the complete replicon particles. Although similar proportions of the same peripheral blood monocyte lineage target cells were infected with the chimeric vs. the complete replicon particles, the latter resulted in enhanced expression of the gene of interest, suggesting a possible mechanism of the enhanced immunogenicity.
Gupta
Soumi
S
Comparative Medicine, University of California, Davis, California 95616, USA.
Zhou
Fengmin
F
Greer
Catherine E
CE
Legg
Harold
H
Tang
Tony
T
Luciw
Paul
P
zur Megede
Jan
J
Barnett
Susan W
SW
Donnelly
John J
JJ
O'Hagan
Derek T
DT
Polo
John M
JM
Vajdy
Michael
M
D000281:Administration, Intranasal; D000818:Animals; D002678:Chimera / Q000276:immunology; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology*; D005260:Female; D015686:Gene Products, env / Q000276:immunology*; D015483:HIV Antibodies / Q000096:biosynthesis*; D018928:Immunity, Mucosal; D007114:Immunization / Q000379:methods*; D007273:Injections, Intramuscular; D008253:Macaca mulatta / Q000276:immunology*; D012093:Replicon / Q000276:immunology*; D012845:Sindbis Virus / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus
D015686:Gene Products, env; D015483:HIV Antibodies; D054299:env Gene Products, Human Immunodeficiency Virus; C097729:gp140 envelope protein, Human immunodeficiency virus 1