Genomic organization, promoter characterization and expression profiles of an antiviral gene PmAV from the shrimp Penaeus monodon.
Diseases caused by viruses are the greatest challenge to worldwide shrimp aquaculture. PmAV is the first antiviral gene identified from the shrimp and its mRNA level is up-regulated in response to viral infection (Luo et al., 2003). The transcriptional regulation of PmAV is critical since the expression of this gene is regulated in response to viral stimuli. In this study, we show that the Penaeus monodon PmAV gene contains four exons, separated by three introns. The 5'-flanking sequence of PmAV gene consists of a typical TATA box, several potential regulatory elements, and a compound microsatellite repeat as well. Interestingly, a TATA box and several regulatory elements also exist in the first intron (intron 1). Both intron 1 and the microsatellite are involved in transcriptional regulation of the PmAV gene. PmAV promoter activity depends on the existence of intron 1 while the microsatellite in 5'-flanking sequence acts as a negative regulative element. In addition, using Real-time PCR, we found that PmAV was highly expressed in the hepatopancreas and was up-regulated from the second day post-infection with the increase of viral load. These results extend our previous findings and provide insights into the molecular regulation of PmAV gene expression, which will be helpful for shrimp viral disease control.
Luo
Tian
T
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), No. 178, Daxue Road, Xiamen, Fujian 361005, PR China.
Li
Fang
F
Lei
Kaiyu
K
Xu
Xun
X
D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D005786:Gene Expression Regulation; D016678:Genome; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics* / Q000821:virology*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D016385:TATA Box; D014018:Tissue Distribution
D012333:RNA, Messenger