title: Detecting the effect of very low amounts of penetrants in lipid bilayers using Raman spectroscopy.
abstract: We show that, by the influence on the gauche content in the acyl chains, the effect of very low concentrations (order 10(-2) %) of penetrants (here 2,4-dichlorophenol) in lipid bilayers (here dipalmitoyl-glycerophosphatidylcholine) can be experimentally seen in the Raman spectra. The observed effects suggest increased order and are in accordance with previous X-ray diffraction and calorimetric data. These findings show great promise for studying in situ the effect of low amounts of various small molecules on cell membranes using Raman spectroscopy.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Meier
authors.forename: Agnes
authors.initials: A
authors.lastname: Csiszár
authors.forename: Erwin
authors.initials: E
authors.lastname: Klumpp
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine; D008051:Lipid Bilayers / Q000378:metabolism*; D009930:Organic Chemicals / Q000493:pharmacokinetics*; D010539:Permeability / Q000187:drug effects; D044367:Phase Transition / Q000187:drug effects*; D013059:Spectrum Analysis, Raman
chemical_list: D008051:Lipid Bilayers; D009930:Organic Chemicals; D015060:1,2-Dipalmitoylphosphatidylcholine