title: Comparison of the in vitro pathogenicity of two Salmonella Typhimurium phage types.
abstract: The in vitro pathogenicity of Salmonella enterica serovar Typhimurium phage type (pt) 90 and pt 506 (also known as DT 104) isolates from human and porcine origin was studied in adhesion and invasion assays to the human cell line Caco-2 and the porcine cell line IPI-2. Interleukin-8 (IL-8) production by these two cell lines in response to stimulation by the two Salmonella phage types was also measured. Generally, Salmonella Typhimurium pt 506 and pt 90 adhered to and invaded Caco-2 cells and IPI-2 cells equally well. The release of IL-8 by Caco-2 cells or by IPI-2 cells was similar, independent of the Salmonella phage type used for stimulation of the cells. These data suggest that Salmonella Typhimurium pt 90 has a similar ability to cause Salmonella infections as Salmonella Typhimurium DT 104.
authors.affiliation: Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80165, 3508 TD Utrecht, The Netherlands.
authors.forename: An T T
authors.initials: AT
authors.lastname: Vo
authors.forename: Engeline
authors.initials: E
authors.lastname: van Duijkeren
authors.forename: Ad C
authors.initials: AC
authors.lastname: Fluit
authors.forename: Henno G C J M
authors.initials: HG
authors.lastname: Hendriks
authors.forename: Peter C J
authors.initials: PC
authors.lastname: Tooten
authors.forename: Wim
authors.initials: W
authors.lastname: Gaastra
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D018938:Caco-2 Cells; D002460:Cell Line; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D012483:Salmonella Phages / Q000472:pathogenicity*; D012486:Salmonella typhimurium / Q000472:pathogenicity* / Q000502:physiology; D013045:Species Specificity; D013552:Swine; D014774:Virulence
chemical_list: D016209:Interleukin-8