The expression patterns of RGS transcripts in platelets.
Regulators of G protein signalling (RGS) are involved in the negative regulation of cell activation processes and are involved in the pathophysiology of cardiovascular diseases. To get some further evidence for a role of RGS proteins in platelets, we determined the expression profile of RGS-specific mRNA in rat platelets using reverse transcription-polymerase chain reaction (RT-PCR) with a poly dT18 primer and transcript-specific primers. We found that RGS2, RGS3, RGS5, RGS6, RGS10, RGS14, RGS16 and RGS18, Leukemia-associated Rho-GEF factor (LARG), and Galpha interacting protein (GAIP) were differentially expressed in platelets. The highest expression rate was found for RGS18 (about 1.3 fold when compared to GAPDH), followed by LARG, RGS6, RGS10 and RGS16 (0.7 to 0.95), whereas expression rates for RGS2, RGS3, RGS5, RGS14, and GAIP were in a range of 0.1 to 0.3. Our results suggest that G-protein-coupled receptor-mediated signalling in platelet may be regulated mainly by RGS 18, 16, 10, 6, and LARG.
Kim
Sung Dae
SD
Laboratory of Veterinary Physiology & Signalling, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
Sung
Hye Jin
HJ
Park
Sun Kyu
SK
Kim
Tae Wan
TW
Park
Seung Chun
SC
Kim
Sang Keun
SK
Cho
Jae Youl
JY
Rhee
Man Hee
MH
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D020662:Guanine Nucleotide Exchange Factors / Q000502:physiology; D020710:RGS Proteins / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D064067:Rho Guanine Nucleotide Exchange Factors; D015398:Signal Transduction
C577224:Arhgef12 protein, rat; D020662:Guanine Nucleotide Exchange Factors; D020710:RGS Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D064067:Rho Guanine Nucleotide Exchange Factors