Direct high-resolution peptide and protein analysis by desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry.
We report the first coupling of a desorption electrospray ionization (DESI) ion source to Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS) for high-resolution protein analysis. The DESI FT-ICR-MS source design is described in detail along with preliminary data obtained on peptides and proteins ranging from 1 to 5.7 kDa.
Bereman
Michael S
MS
W M Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
Nyadong
Leonard
L
Fernandez
Facundo M
FM
Muddiman
David C
DC
D001920:Bradykinin / Q000032:analysis; D005561:Formates / Q000032:analysis; D005934:Glucagon / Q000032:analysis; D007328:Insulin / Q000032:analysis; D008555:Melitten / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D014665:Vasodilator Agents / Q000032:analysis
D005561:Formates; D007328:Insulin; D010455:Peptides; D011506:Proteins; D014665:Vasodilator Agents; C030544:formic acid; D008555:Melitten; D005934:Glucagon; D001920:Bradykinin