title: Effect of sodium [36Cl]chlorate dose on total radioactive residues and residues of parent chlorate in growing swine.
abstract: An experimental chlorate-based product has been shown to be efficacious in eliminating economically important, Gram-negative human pathogens in the gastrointestinal tracts of food animals. Prior to the commercial marketing of such a product, the magnitude and chemical nature of residues remaining in edible tissues must be determined. Thus, the objective of this study was to determine the tissue distribution and elimination of sodium [36Cl]chlorate in orally dosed swine. Three sets of pigs, each consisting of a barrow and a gilt, were orally dosed with a total of 20, 40, or 60 mg of sodium [36Cl]chlorate per kg body weight via the drinking water. Urine and feces were collected throughout the 30 h study. Twenty-four hours after the last exposure to [36Cl]chlorate, each pig was harvested and both edible and inedible tissues were collected. Urine and tissue samples were analyzed for total radioactive residues and for chlorate metabolites. Elimination of radioactivity in urine averaged 81.6, 83.7, and 83.9% of the total dose for the low, medium, and high doses, respectively. Fecal elimination of radioactivity averaged 1.1% of the dosed radiochlorine across all doses. Parent chlorate always represented greater than 97.4% of the urinary radiochlorine with the remaining radiochlorine being excreted as chloride ion. Chlorate represented 39-77% of fecal radioactivity, depending upon dose. Chlorate concentrations in edible tissues ranged from 0.01 to 0.49 ppm, with residues in liver and skeletal muscle generally lower than those in kidney and adipose tissue. Chlorate residues were concentrated in thyroid tissues (7.7-25.4 ppm) relative to edible tissues. No evidence for the presence of chlorite was observed in excreta or in tissues. Results of this study suggest that further development of chlorate as a preharvest food safety tool in swine merits consideration.
authors.affiliation: Biosciences Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Fargo, North Dakota 58105-5674, USA. smithd@fargos.ars.usda.gov
authors.forename: D J
authors.initials: DJ
authors.lastname: Smith
authors.forename: R C
authors.initials: RC
authors.lastname: Anderson
authors.forename: J K
authors.initials: JK
authors.lastname: Huwe
mesh_terms: D000818:Animals; D002704:Chlorates / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D002713:Chlorine / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics*; D004350:Drug Residues / Q000032:analysis* / Q000493:pharmacokinetics; D005243:Feces / Q000737:chemistry; D011868:Radioisotopes / Q000032:analysis / Q000493:pharmacokinetics; D013552:Swine / Q000254:growth & development*; D014867:Water
chemical_list: D002704:Chlorates; D011868:Radioisotopes; D014867:Water; D002713:Chlorine; C032706:sodium chlorate