The Bysl gene product, bystin, is essential for survival of mouse embryos.
Human bystin is a cytoplasmic protein directly binding to trophinin, a cell adhesion molecule potentially involved in human embryo implantation. The present study shows that bystin is expressed in luminal and glandular epithelia in the mouse uterus at peri-implantation stages. In fertilized embryos, bystin was not seen until blastocyst stage. Bystin expression started during hatching and increased in expanded blastocyst. However, bystin apparently disappeared from the blastocyst during implantation. After implantation bystin re-appeared in the epiblast. Targeted disruption of the mouse bystin gene, Bysl, resulted in embryonic lethality shortly after implantation, indicating that bystin is essential for survival of mouse embryos.
Aoki
Rui
R
Cancer Research Center, Burnham Institute for Medical Research, La Jolla, CA, USA.
Suzuki
Nao
N
Paria
Bibhash C
BC
Sugihara
Kazuhiro
K
Akama
Tomoya O
TO
Raab
Gerhard
G
Miyoshi
Masaya
M
Nadano
Daita
D
Fukuda
Michiko N
MN
D000818:Animals; D001755:Blastocyst / Q000737:chemistry; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D010064:Embryo Implantation; D047108:Embryonic Development / Q000235:genetics*; D004848:Epithelium / Q000737:chemistry; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D013534:Survival; D014162:Transfection; D014599:Uterus / Q000737:chemistry
C504465:Bysl protein, mouse; D015815:Cell Adhesion Molecules