Modulated expression of cell surface molecules and in vivo outgrowth of modified melanoma cells.
Modulation of cell surface molecules involved in immune recognition and cellular interactions (class I major histocompatibility complex or MHC-I, B7.1 or CD80, integrin alpha4 or CD49d, tetraspanins CD9, CD81) was examined in modified B16 melanoma cells displaying either inhibited IGF-I expression or transfected OVA encoding gene. It was shown that inhibiting IGF-I expression or inserting OVA encoding gene did not lead to modification relevant to the presence of MHC-I or B7.1. However downregulation of tetraspanin CD9 was observed in modified IGF-I but not in OVA encoding gene inserted melanoma cells. Expression of tetraspanin CD81 and integrin alpha4/CD49d remained unchanged. Inoculated into syngeneic recipients, the modified melanoma cells exhibited significant delayed outgrowth with a reduction in the percentage of lethal tumors observed essentially in hosts injected with inhibited IGF-I expression cells.
Trabado
S
S
Laboratoire de Toxicologie, Faculté de Pharmacie, Université René-Descartes Paris-V, Paris, France.
Nguyen Van Binh
P
P
Martin
C
C
Lafarge-Frayssinet
C
C
Thevenin
M
M
Baudouin
F
F
Warnet
J-M
JM
Duc
H-T
HT
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D018122:B7-1 Antigen / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016373:DNA, Antisense / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects; D018274:Electroporation / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism; D006921:Hygromycin B / Q000494:pharmacology; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D039441:Integrin alpha4 / Q000235:genetics / Q000378:metabolism; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000235:genetics / Q000378:metabolism; D060225:Tetraspanin 28; D060245:Tetraspanin 29; D014162:Transfection / Q000379:methods
D000900:Anti-Bacterial Agents; D015703:Antigens, CD; D000954:Antigens, Surface; D018122:B7-1 Antigen; C554799:Cd81 protein, mouse; C554870:Cd9 protein, mouse; D016373:DNA, Antisense; D015395:Histocompatibility Antigens Class I; D008562:Membrane Glycoproteins; D060225:Tetraspanin 28; D060245:Tetraspanin 29; D039441:Integrin alpha4; D006921:Hygromycin B; D007334:Insulin-Like Growth Factor I; D010047:Ovalbumin