title: Fluorescence in situ hybridization in cardiovascular disease.
abstract: Many human diseases are associated with cytogenetic abnormalities or chromosomal disorders including translocations, deletions, duplications, inversions, and other complicated chromosomal changes. Fluorescence in situ hybridization (FISH), a technique involving hybridization of labeled probes to chromosomes and detection of hybridization via fluorochromes, has become a popular method for identification and characterization of cytogenetic abnormalities. For FISH analysis, metaphase chromosomes are prepared by mitotic arrest and hypotonic shock, and denatured. Hybridization of digoxigenin- or biotin-labeled probes to these chromosomes is visualized using fluorochromes like fluorescein isothiocyanate and Texas Red. We have successfully applied FISH technology to the characterization of chromosome breakpoints involved in disease-associated cytogenetic abnormalities to identify candidate gene(s) for the disease. FISH is also widely used in clinical diagnosis of chromosomal disorders.
authors.affiliation: Department of Molecular Cardiology, The Cleveland Clinic Foundation, OH, USA.
authors.forename: Ayse Anil
authors.initials: AA
authors.lastname: Timur
authors.forename: Qing K
authors.initials: QK
authors.lastname: Wang
mesh_terms: D000818:Animals; D002318:Cardiovascular Diseases / Q000175:diagnosis* / Q000235:genetics; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D025063:Chromosome Disorders / Q000175:diagnosis / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D051379:Mice