Genotoxicity in primary human peripheral lymphocytes after exposure to regular and white mineral trioxide aggregate.
OBJECTIVE
Taking into consideration that DNA damage plays an important role in carcinogenesis, the purpose of this study was to evaluate whether regular and white mineral trioxide aggregate (MTA) are able to induce genetic damage in primary human cells.


METHODS
Human peripheral lymphocytes obtained from 10 healthy volunteers were exposed to 2 presentation forms of MTA at final concentrations ranging from 1 to 1000 microg/mL for 1 hour at 37 degrees C. The negative control group was treated with vehicle control (phosphate buffer solution, PBS) for 1 hour at 37 degrees C and the positive control group was treated with hydrogen peroxide (at 100 microM) for 5 minutes on ice. Results were analyzed by the Friedman nonparametric test.


RESULTS
The results pointed out that either regular or white MTA in all concentrations tested did not induce DNA breakage in human peripheral lymphocytes as depicted by the mean tail moment.


CONCLUSIONS
In summary, our results indicate that exposure to MTA may not be a factor that increases the level of DNA lesions in human peripheral lymphocytes as detected by single cell gel (comet) assay.
da Silva
Glenda N
GN
Research program, Department of Pathology, São Paulo State University, Sao Paulo, Brazil.
Braz
Mariana G
MG
de Camargo
Elaine A
EA
Salvadori
Daisy M F
DM
Ribeiro
Daniel A
DA
D000328:Adult; D017607:Aluminum Compounds / Q000633:toxicity*; D017610:Calcium Compounds / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage; D004338:Drug Combinations; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D010087:Oxides / Q000633:toxicity*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D018709:Statistics, Nonparametric
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate