A p53 genetic polymorphism of gastric cancer: difference between early gastric cancer and advanced gastric cancer.
OBJECTIVE
To investigate the role of the polymorphism of p53 codon 72 in early gastric cancer (EGC) and advanced gastric cancer (AGC) in Korean patients.


METHODS
DNA was extracted from blood samples of gastric cancer patients (n=291) and controls (n=216). In the p53 codon 72 genotypes were determined by PCR-RFLP.


RESULTS
Patients with gastric cancer had a significantly higher frequency of the homozygous proline (Pro) allele than the control (P=0.032). Patients with AGC had a significantly higher frequency of the Arg/Arg (arginine) allele (P=0.038) than EGC and a similar Pro/Pro allele. The signet ring cell type had a higher frequency of the Pro/Pro allele than other types (P=0.031). The Pro/Pro genotype carries a 3.9-fold increased risk of developing gastric cancer (95% CI, 1.3-15.4, P=0.039) when compared to Arg/Arg and Arg/Pro genotypes and to develop EGC is a 5.25 fold increased risk (95% CI, 1.8-19.6, P=0.021).


CONCLUSIONS
The Pro/Pro genotype of the p53 codon 72 polymorphism carries a higher risk for gastric cancer in general and is also associated with a much higher risk for EGC than AGC.
Yi
Sun Young
SY
Department of Internal Medicine and Medical Research Center, College of Medicine, Ewha Womans University, Seoul, Korea. syy@ewha.ac.kr
Lee
Woon Jung
WJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D018450:Disease Progression; D005260:Female; D005838:Genotype; D006801:Humans; D007723:Korea; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011110:Polymorphism, Genetic; D011392:Proline / Q000032:analysis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000235:genetics*
D004273:DNA, Neoplasm; D016159:Tumor Suppressor Protein p53; D011392:Proline