Bone repair of rabbit mandibular transsection using rhBMP-2 and atelocollagen gel.
This experimental was carried out using 12 rabbits. A rabbit experimental mandibular transsection was reconstructed with 10mug of rhBMP-2 and 1% atelocollagen gel. The transsection gap was fixed with a titanium plate and screws, and covered with poly (lactic-co-glycolic acid) co-polymer (PLGA) membrane. Histopathological examination of 1-week specimens revealed that many spindle cells had proliferated and invaded blood clots, and a small amount of immature trabecular bone was formed in the transsection gap. In 2 and 3-week specimens, bone formation was gradually increased in the fibro-vascular tissues of the site. These histological findings were also observed in the control group specimens, but the bone formation was slightly less than in the experimental group. The results suggest the effectiveness of atelocollagen gel as a carrier of rhBMP-2 and PLGA as a covering membrane in this rabbit mandibular transsection reconstruction model.
Kimura
A
A
Department of Oral Pathology, Matsumoto Dental University School of Dentistry, 1780 Hirooka-Gobara, Shiojiri 399-0781, Japan. akihiro@po.mdu.ac.jp
Watanabe
T
T
Shimizu
T
T
Okafuji
N
N
Mori
R
R
Furusawa
K
K
Kawai
T
T
Hasegawa
H
H
Kawakami
T
T
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D003094:Collagen / Q000008:administration & dosage; D004337:Drug Carriers / Q000008:administration & dosage; D005782:Gels; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008334:Mandible / Q000187:drug effects / Q000254:growth & development / Q000502:physiology* / Q000601:surgery*; D008954:Models, Biological; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D011091:Polyesters; D011100:Polyglycolic Acid / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D013997:Time Factors; D014025:Titanium / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*; D016896:Treatment Outcome
C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; D005782:Gels; D011091:Polyesters; D011108:Polymers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C059821:atelocollagen; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; D014025:Titanium