Regulation of alpha-smooth muscle actin protein expression in adipose-derived stem cells.
The objective of this work was to study the response of adipose-derived stem cells (ASCs) to exogenous biochemical stimulation, and the potential of ASCs to differentiate toward the smooth muscle cell (SMC) lineage. Immunofluorescence staining and Western blot analysis detected protein expression of the early SMC marker alpha-smooth muscle actin (alpha-SMA) in both control and experiment groups. Expression of alpha-SMA in ASCs significantly increased when treated with transforming growth factor-beta1, while alpha-SMA expression only slightly increased in the presence of retinoic acid (RA), beta-mercaptoethanol and ascorbic acid. Treatment with platelet-derived growth factor-BB, RA and dibutyryl-cyclic adenosine monophosphate decreased the expression of alpha-SMA significantly. While beta-mercaptoethanol and ascorbic acid, as well as RA have resulted in increased alpha-SMA expression in marrow-derived mesenchymal stem cells and other progenitor cells, our results demonstrate that these treatments do not significantly increase alpha-SMA expression, indicating that the differentiation potential of ASCs and mesenchymal stem cells may be fundamentally different.
Lee
Wen-Chi C
WC
Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Rubin
J Peter
JP
Marra
Kacey G
KG
D000199:Actins / Q000235:genetics* / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology*; D001205:Ascorbic Acid / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D019085:Fluorescent Antibody Technique, Direct; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008623:Mercaptoethanol / Q000494:pharmacology; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D000199:Actins; D016895:Culture Media, Serum-Free; D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; D014212:Tretinoin; D008623:Mercaptoethanol; D003994:Bucladesine; D001205:Ascorbic Acid