title: Autoantigen arrays for multiplex analysis of antibody isotypes.
abstract: We describe here a microarray-based method for multiplexed, antigen-specific assessment of immunoglobulin (Ig) subclasses. We used 1152-feature arrays composed of 140 antigens or antigen fragments to detect isotype-specific mAb, to quantitatively monitor changes in isotype mAb concentration, and to profile antigen-specific antibody isotype production in a murine model of autoimmunity. This platform can be easily adapted to a variety of applications, and has the potential to elucidate mechanisms that govern development and evolution of antibody responses in in vivo and in vitro systems.
authors.affiliation: Department of Medicine, Division of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: Kareem L
authors.initials: KL
authors.lastname: Graham
authors.forename: Maria
authors.initials: M
authors.lastname: Vaysberg
authors.forename: Annie
authors.initials: A
authors.lastname: Kuo
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Utz
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D005069:Evaluation Studies as Topic; D007074:Immunoglobulin G / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000032:analysis* / Q000276:immunology; D051379:Mice; D040081:Protein Array Analysis
chemical_list: D000911:Antibodies, Monoclonal; D001324:Autoantigens; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes