Recombinant canarypox virus vaccine co-expressing genes encoding the VP2 and VP5 outer capsid proteins of bluetongue virus induces high level protection in sheep.
We describe the development and preliminary characterization of a recombinant canarypox virus vectored vaccine for protective immunization of ruminants against bluetongue virus (BTV) infection. Sheep (n=6) immunized with recombinant canarypox virus vector (BTV-CP) co-expressing synthetic genes encoding the two outer capsid proteins (VP2 and VP5) of BTV serotype 17 (BTV-17) developed high titers (40-160) of virus-specific neutralizing antibodies and were resistant to challenge with a field strain of BTV-17. In contrast, sheep (n=5) immunized with a commercial recombinant canarypox virus vector expressing the E and preM genes of West Nile virus were seronegative to BTV and developed pyrexia, lymphopenia, and extended, high-titered viremias following challenge exposure to the field strain of BTV-17. These data confirm that the BTV-CP vaccine may be useful for the protective immunization of ruminants against bluetongue, and it may avoid the problems inherent to live-attenuated (LA) BTV vaccines.
Boone
Josh D
JD
Equine Viral Disease Laboratory, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Balasuriya
Udeni B
UB
Karaca
Kemal
K
Audonnet
Jean-Christophe
JC
Yao
Jiansheng
J
He
Ling
L
Nordgren
Robert
R
Monaco
Federica
F
Savini
Giovanni
G
Gardner
Ian A
IA
Maclachlan
N James
NJ
D000818:Animals; D001819:Bluetongue / Q000517:prevention & control*; D001820:Bluetongue virus / Q000276:immunology / Q000378:metabolism*; D028121:Canarypox virus / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins / Q000276:immunology* / Q000378:metabolism; D005260:Female; D015967:Gene Expression Regulation, Viral; D008297:Male; D012756:Sheep; D013997:Time Factors; D014765:Viral Vaccines / Q000276:immunology*
D036022:Capsid Proteins; C442520:VP2 protein, Bluetongue virus; C488468:VP5 protein, Bluetongue virus; D014765:Viral Vaccines