Apoptosis of T-leukemia and B-myeloma cancer cells induced by hyperbaric oxygen increased phosphorylation of p38 MAPK.
Tumor cells with different origins have different threshold to apoptosis. Hematopoietic (Jurkat, NCI-H929) cells and non-hematopoietic (A549, MCF-7) cells were received hyperbaric oxygen (HBO(2)) treatment from 2.5 to 3.5 atmosphere absolute (ATA) of 100% oxygen for 6h, and a significant percentage of apoptosis were shown only in hematopoietic Jurkat and NCI-H929 cells by either Annexin V or TUNEL assay. Oxidative stress was illustrated higher in HBO(2)-treated hematopoietic cells by superoxide fluorochrome detectors. HBO(2) treatment leads to caspase-3, caspase-7 activation and further cleavage of PARP within cells. Furthermore, the increased phosphorylation of p38 MAPK was demonstrated in both Jurkat and NCI-H929 cells.
Chen
Yen-Chen
YC
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC.
Chen
Shao-Yuan
SY
Ho
Pey-Shen
PS
Lin
Chia-Huei
CH
Cheng
Ya-Yuan
YY
Wang
Jehng-Kang
JK
Sytwu
Huey-Kang
HK
D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D001402:B-Lymphocytes / Q000201:enzymology / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D024161:Collagen Type XI / Q000378:metabolism; D006801:Humans; D006931:Hyperbaric Oxygenation; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000201:enzymology* / Q000473:pathology / Q000628:therapy; D009101:Multiple Myeloma / Q000201:enzymology* / Q000473:pathology / Q000628:therapy; D010100:Oxygen / Q000378:metabolism; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D017304:Annexin A5; C499319:COL11A2 protein, human; D024161:Collagen Type XI; D048051:p38 Mitogen-Activated Protein Kinases; C505520:CASP7 protein, human; D053148:Caspase 3; D053179:Caspase 7; D010100:Oxygen