[The anti-respiratory syncytial virus effect of active compound of Glycyrrhiza GD4 in vitro].
OBJECTIVE
To study the effect on anti-respiratory syncytial virus of an active compound GD4 from Glycyrrhiza in vitro.


METHODS
GD4 was extracted from Glycyrrhiza by hot water extraction, dichloromethane extraction and column chromatography. The CPE inhibition assay was used to test the antiviral activity of GD4 on RSV in Hela cells.


RESULTS
GD4 was effective antiviral agent for RSV in a concentration-dependent manner and its median toxic concentration (TC50) and median effeicacious concentration (EC50) and treatment index (TI) was 0.23 mg/ml, 28.73 microg/ml and 8.0. 120 microg/ml GD4 exhibited inhibitory effect when added into cell culture for every 2h within 12h post-infection. GD4 didn't contain glycyrrhizic acid.


CONCLUSIONS
The active compound of Glycyrrhiza (GD4) was an evident inhibitory effect on RSV.
Wang
Xiu-qin
XQ
Public Health College of Harbin Medical University, China.
Li
Hong-yuan
HY
Liu
Xin-yan
XY
Zhang
Feng-mei
FM
Li
Xia
X
Piao
Ying-ai
YA
Xie
Zhi-ping
ZP
Chen
Zhen-hua
ZH
Li
Xin
X
D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D006035:Glycyrrhiza / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D010946:Plants, Medicinal / Q000737:chemistry*; D012136:Respiratory Syncytial Viruses / Q000187:drug effects*; D012254:Ribavirin / Q000008:administration & dosage / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D012254:Ribavirin