Quantitative analysis of cytochrome C released from rice mitochondria using the adsorptive polarographic wave of guanidine modified Co(II)-cytochrome C complex.
A new, simple and sensitive method for the quantitative analysis of cytochrome C (Cyt C) based on the reduction wave of guanidine modified Co(II)-Cyt C complex at about -1.74 V (vs. SCE) by single sweep polarography in the solution containing 8 x 10(-6) mol L(-1) CoCl2, 0.04 mol L(-1) guanidine hydrochloride, 0.2 mol L(-1) NaOH and 0.5% Na2SO3. The peak height is linearly proportional to the concentration of Cyt C in the range of 0.005 approximately 1.500 mg L(-1) (correlation coefficient 0.999). Common amino acids, saccharide, organic acid and metal ions of appropriate concentrations have no interference on the Cyt C determination. The released Cyt C in the process of mitochondrial permeability transition of Hong-Lian cytoplasmic male sterile line of rice has been measured by the method, and the result is satisfactory.
Xia
Chunjiao
C
Key Lab for Biotechnology of National Commission for Nationalities, South-Central University for Nationalities, Wuhan 430074, P R China.
Liu
Xuequn
X
Luo
Dengbai
D
Wang
Hongqiang
H
He
Fengqin
F
Yuan
Lei
L
Wang
Chuntai
C
D000596:Amino Acids / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry; D045304:Cytochromes c / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D019791:Guanidine / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism*; D009005:Monosaccharides / Q000737:chemistry; D012275:Oryza / Q000378:metabolism*; D011048:Polarography / Q000379:methods*; D015203:Reproducibility of Results; D012972:Sodium Hydroxide / Q000737:chemistry; D013447:Sulfites / Q000737:chemistry
D000596:Amino Acids; D009005:Monosaccharides; D013447:Sulfites; D003035:Cobalt; D012972:Sodium Hydroxide; D045304:Cytochromes c; C018021:cobaltous chloride; D019791:Guanidine; C025026:sodium sulfite