Major histocompatibility polymorphism associated with resistance towards amoebic gill disease in Atlantic salmon (Salmo salar L.).
The association between major histocompatibility (MH) polymorphism and the severity of infection by amoebic gill disease (AGD) was investigated across 30 full sibling families of Atlantic salmon. Individuals were challenged with AGD for 19days and then their severity of infection scored by histopathological examination of the gills. Fish were then genotyped for the MH class I (Sasa-UBA) and MH class II alpha (Sasa-DAA) genes using polymorphic repeats embedded within the 3' untranslated regions of the Sasa-UBA and Sasa-DAA genes. High variation in the severity of infection was observed across the sample material, ranging from 0% to 85% gill filaments infected. In total, seven Sasa-DAA-3UTR and ten Sasa-UBA-3UTR marker alleles were identified across the 30 families. A significant association between the marker allele Sasa-DAA-3UTR 239 and a reduction in AGD severity was detected. There was also a significant association found between AGD severity and the presence of two Sasa-DAA-3UTR genotypes. While the associations between MH allele/genotypes and AGD severity reported herein may be statistically significant, the small sample sizes observed for some alleles and genotypes means these associations should be considered as suggestive and future research is required to verify their biological significance.
Wynne
James W
JW
CSIRO Marine and Atmospheric Research, CSIRO Food Futures Flagship, Hobart 7001, Tasmania, Australia. james.wynne@csiro.au
Cook
Mathew T
MT
Nowak
Barbara F
BF
Elliott
Nicholas G
NG
D000818:Animals; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D005880:Gills / Q000469:parasitology / Q000473:pathology; D007113:Immunity, Innate / Q000235:genetics; D016840:Lobosea / Q000502:physiology; D011110:Polymorphism, Genetic; D011528:Protozoan Infections / Q000276:immunology; D011529:Protozoan Infections, Animal; D020317:Salmo salar / Q000235:genetics* / Q000276:immunology*; D012720:Severity of Illness Index