Recent advances in all-protein chromophore technology.
The green fluorescent protein (GFP) is the foundation of a powerful technology that has revolutionized the way in which the life scientist carries out experiments in the living cell. The technology is continually evolving and improving through the development of existing proteins and discovery of new members of the all-protein chromophore (APC) family. This review gives an overview of the more recent advances in the technology with a particular focus on APCs having optical properties that are significantly red-shifted relative to those variants derived from Aequorea victoria GFP.
Prescott
Mark
M
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria 3800, Australia. Mark.Prescott@med.monash.edu.au
Battad
Jion M
JM
Wilmann
Pascal G
PG
Rossjohn
Jamie
J
Devenish
Rodney J
RJ
D001709:Biotechnology / Q000379:methods*; D036681:Fluorescence Recovery After Photobleaching; D031541:Fluorescence Resonance Energy Transfer; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods; D013050:Spectrometry, Fluorescence
D008164:Luminescent Proteins