title: Preparation and properties of immobilized pectinase onto the amphiphilic PS-b-PAA diblock copolymers.
abstract: Well-defined amphiphilic block copolymers poly(styrene-b-acrylic acid) (PS-b-PAA) with controlled block length were synthesized using atom transfer radical polymerization (ATRP). Pectinase enzyme was immobilized on the well-defined amphiphilic block copolymers PS-b-PAA. The carboxyl groups on the amphiphilic PS-b-PAA diblock copolymers present a very simple, mild, and time-saving process for enzyme immobilization. Various characteristics of immobilized pectinase such as the pH and temperature stability, thermal stability, and storage stability were valuated. Among them the pH optimum and temperature optimum of free and immobilized pectinase were found to be pH 6.0 and 65 degrees C.
authors.affiliation: College of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China. zhllei@snnu.edu.cn
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Lei
authors.forename: Shuxian
authors.initials: S
authors.lastname: Bi
mesh_terms: D000179:Acrylates / Q000138:chemical synthesis*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011096:Polygalacturonase / Q000378:metabolism*; D011108:Polymers / Q000138:chemical synthesis*; D011137:Polystyrenes / Q000138:chemical synthesis*; D013696:Temperature
chemical_list: D000179:Acrylates; D004800:Enzymes, Immobilized; D011108:Polymers; D011137:Polystyrenes; C509855:poly(styrene-b-acrylic acid); D011096:Polygalacturonase