[A comparison study on clinical chromatographic fingerprintings of mycolic acids analysis of 18 standard strains of Mycobacteria in China].
OBJECTIVE
A clinical chromatographic fingerprinting of 18 standard strains of Mycobacteria commercialized in China by high-performance liquid chromatography approach was developed and improved in this laboratory. A comparison of clinical chromatographic fingerprintings of mycolic acids was carried out.


METHODS
Mycolic acids were extracted from whole cultured cells of Mycobacteria by biochemical methods, and then samples was prepared for analysis after the mycolic acids were saponified, acidified and derived. The samples were detected by high-performance liquid chromatography under optimum conditions.


RESULTS
Using above techniques a clinical chromatographic fingerprinting of Mycolic acids analysis was successfully established. The repeatability of the mycolic acids fingerprinting was evaluated. Coefficient of variation (CV) of chromatographic fingerprinting was between 0.20% - 0.83%.


CONCLUSIONS
The first clinical chromatographic fingerprinting of 18 standard strains of Mycobacteria in China was established in this laboratory. Mycobacteria can be accurately identified according to chromatographic fingerprinting in a short time. Moreover, there was significant difference between BCG vaccine and Mycobacterium bovis when their peak was compared, which may lead to research of new tools for identifying insidious infection of tuberculosis and for the study of the mechanism of drug tolerance.
Zhao
Feng-yi
FY
Orthopaedic Institute of Tianjin Hospital, Tianjin 300211, China.
Wang
Ju-cun
JC
Wang
Xie-xiu
XX
Zhao
De-fu
DF
Bai
Ren-xiao
RX
Wang
Su-min
SM
Wang
Xue-qian
XQ
Xing
Guo-sheng
GS
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification; D009171:Mycolic Acids / Q000032:analysis*; D010449:Peptide Mapping
D009171:Mycolic Acids