Expression and distribution of Gpr119 in the pancreatic islets of mice and rats: predominant localization in pancreatic polypeptide-secreting PP-cells.
The GPR119 was recently shown to be activated by oleoylethanolamide (OEA), a naturally occurring bioactive lipid with hypophagic and anti-obesity effects. In this study, we have cloned and characterized its murine counterpart, Gpr119. The full-length cDNA contained an open reading frame of 1008bp encoding a 335-amino acid protein. The genomic organization of Gpr119 was unique, having a 3'-untranslated second exon that was also involved in an alternative splicing event. Gene expression analyses confirmed its specific expressions in pancreatic islets and two endocrine cell-lines, MIN6 and alphaTC1. Immunohistochemistry and double-immunofluorescence studies using a specific antibody revealed the predominant Gpr119 localization in pancreatic polypeptide (PP)-cells of islets. No definitive evidence of Gpr119-immunoreactivity in adult beta- or alpha-cells was obtained. The Gpr119 mRNA levels were elevated in islets of obese hyperglycemic db/db mice as compared to control islets, suggesting a possible involvement of this receptor in the development of obesity and diabetes.
Sakamoto
Yukiko
Y
Institute for Genome Research, The University of Tokushima, Tokushima, Japan.
Inoue
Hiroshi
H
Kawakami
Shuhei
S
Miyawaki
Katsuyuki
K
Miyamoto
Tatsuro
T
Mizuta
Kuniko
K
Itakura
Mitsuo
M
D020413:3' Untranslated Regions; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D005091:Exons; D006943:Hyperglycemia / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D051379:Mice; D008820:Mice, Obese; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D050418:Pancreatic Polypeptide-Secreting Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D020413:3' Untranslated Regions; C516207:Gpr119 protein, mouse; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins