[Effect of 2-methoxycinnamaldehyde on activity of COX and PGE2 release in cerebral microvascular endothelial cells stimulated by IL-1].
OBJECTIVE
To observe the effect of 2-methoxycinnamaldehyde (isolated from fraction A of Guizhi Tang) on activity of COX and PGE2 release in rat cerebral microvascular endothelial cells (rCMEC) stimulated by IL-1.


METHODS
rCMEC were cultured, and identified by immunohistochemistry for von Willebrand factor (VIII factor, a marker for all endothelial cells) in cytoplasm of the cells. Different concentrations of 2-methoxycinnamaldehyde were added respectively and incubated for 3 hours, then stimulated for another 12 hours by IL-1. Activities of COX-1 and COX-2 in rCMEC, and production of PGE2 in the conditioned media were measured by ELISA.


RESULTS
Positive immunostaining for VIII factor was present diffusely in the cytoplasm of > 90% rCMEC. After being exposed to 30 ng x mL(-1) IL, the activity of COX-2 in rCMEC and the production of PGE2 in conditioned media were higher than those of control group, while there was no difference on activity of COX-1 in the two groups. 2-methoxycinnamaldehyde could down-regulate them in concentration-dependently, and significant differences on the activity of COX-2 and amount of PGE2 were showed in 200 microg x mL(-1) concentration.


CONCLUSIONS
2-methoxycinnamaldehyde can affect the PGE2 release in rCMEC induced by IL-1, which might be related with its inhibition on the activity of COX-2.
Guo
Jian-you
JY
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing.
Yang
Yuan-xiao
YX
Zhao
Bao-sheng
BS
Liu
Hong-bin
HB
Li
Lan-fang
LF
Ma
Yue-Ying
YY
Guo
Shu-ying
SY
Huo
Hai-ru
HR
Jiang
Ting-liang
TL
D000171:Acrolein / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000098:blood supply*; D002478:Cells, Cultured; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D007375:Interleukin-1 / Q000037:antagonists & inhibitors; D008297:Male; D008833:Microcirculation / Q000166:cytology; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; C017910:2-methoxycinnamaldehyde; D000171:Acrolein; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D015232:Dinoprostone