Comparison between basal and apical dendritic spines in estrogen-induced rapid spinogenesis of CA1 principal neurons in the adult hippocampus.
Modulation of hippocampal synaptic plasticity by estrogen has been attracting much attention. Here, we demonstrated the rapid effect of 17beta-estradiol on the density and morphology of spines in the stratum oriens (s.o., basal side) and in the stratum lacunosum-moleculare (s.l.m., apical side) by imaging Lucifer Yellow-injected CA1 neurons in adult male rat hippocampal slices, because spines in s.o. and s.l.m. have been poorly understood as compared with spines in the stratum radiatum. The application of 1nM estradiol-induced a rapid increase in the density of spines of pyramidal neurons within 2h. This increase by estradiol was blocked by Erk MAP kinase inhibitor and estrogen receptor inhibitor in both regions. Effect of blockade by agonists of AMPA receptors and NMDA receptors was different between s.o. and s.l.m. In both regions, ERalpha agonist PPT induced the same enhancing effect of spinogenesis as that induced by estradiol.
Murakami
Gen
G
Department of Biophysics and Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153, Japan.
Tsurugizawa
Tomokazu
T
Hatanaka
Yusuke
Y
Komatsuzaki
Yoshimasa
Y
Tanabe
Nobuaki
N
Mukai
Hideo
H
Hojo
Yasushi
Y
Kominami
Shiro
S
Yamazaki
Takeshi
T
Kimoto
Tetsuya
T
Kawato
Suguru
S
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000818:Animals; D049229:Dendritic Spines / Q000187:drug effects / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D009473:Neuronal Plasticity; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009570:Nitriles / Q000494:pharmacology; D010636:Phenols; D017966:Pyramidal Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D011720:Pyrazoles / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000819:agonists / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists / Q000378:metabolism; D011422:Propionates
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D004958:Estradiol; D047628:Estrogen Receptor alpha; D048049:Extracellular Signal-Regulated MAP Kinases; D005419:Flavonoids; D009570:Nitriles; D010636:Phenols; D011720:Pyrazoles; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; C486182:2,3-bis(4-hydroxyphenyl)-propionitrile; D011422:Propionates; C486184:4,4',4''-(4-propyl-((1)H)-pyrazole-1,3,5-triyl) tris-phenol; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one