title: Enterobacterial 38-kDa outer membrane protein is an age-dependent molecular marker of innate immunity and immunoglobulin deficiency as results from its reactivity with IgG and IgA antibody.
abstract: In earlier studies on an animal model we observed protective properties of outer membrane proteins (OMPs) of Shigella, Hafnia, and Escherichia coli strains. In order to investigate human sera for reactivity with OMPs we subjected these proteins to immunoblotting with umbilical cord plasma and sera from children and adults. The IgG and IgA antibodies interacted primarily with a 38-kDa protein, in similar way for several enterobacterial strains, but different for Pseudomonas aeruginosa. This observation prompted us to determine the reactivity with the purified 38-kDa OMP in the sera of several groups of children. The reactivity of the protein from Shigella flexneri serotype 3a with sera in ELISA was age dependent, increasing from low reactivity in infants to the adult antibody level. The IgG and IgA antibody specific response thus revealed the normal pattern of immunity. The level of IgA and IgG antibody was significantly low in child patients with IgA and/or IgG immunoglobulin deficiencies, but was at the healthy control level in children with recurrent respiratory tract inflammation. These data correlated with total IgA and IgG levels in immunoglobulin-deficient children. The results indicate that this protein may serve as an immunodiagnostic marker, but also as an antigen carrier in vaccines.
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland. witkows@iitd.pan.wroc.pl
authors.forename: Danuta
authors.initials: D
authors.lastname: Witkowska
authors.forename: Ewa
authors.initials: E
authors.lastname: Masłowska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Staniszewska
authors.forename: Bernadeta
authors.initials: B
authors.lastname: Szostko
authors.forename: Adam
authors.initials: A
authors.lastname: Jankowski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D015415:Biomarkers; D002648:Child; D002675:Child, Preschool; D004755:Enterobacteriaceae / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D017098:IgA Deficiency / Q000097:blood / Q000276:immunology; D017099:IgG Deficiency / Q000097:blood / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology*; D015151:Immunoblotting / Q000379:methods; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007223:Infant; D008875:Middle Aged; D011550:Pseudomonas aeruginosa / Q000276:immunology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D015415:Biomarkers; D007070:Immunoglobulin A; D007074:Immunoglobulin G