Neural transplantation of human MSC and NT2 cells in the twitcher mouse model.
BACKGROUND
Accumulating evidence has demonstrated that the NT2 embryonal carcinoma cell line and multipotential stem cells found in BM, mesenchymal stromal cells (MSC), have the ability to differentiate into a wide variety of cell types. This study was designed to explore the efficacy of these two human stem cell types as a graft source for the treatment of demyelinating disorders such as Krabbe's disease and multiple sclerosis (MS).


METHODS
We examined the engraftment and in vivo differentiation of adult MSC and NT2 cells after transplantation into two demyelinating environments, the neonatal and postnatal twitcher mouse brain.


RESULTS
Both types of xenografts led to anatomical integration, without tumor formation, and remained viable in the normal and twitcher mouse brain, showing differentiation into neurons, astrocytes and oligodendrocytes.


CONCLUSIONS
This study represents a platform for further stem cell transplantation studies in the twitcher model and potentially has important therapeutic implications.
Croitoru-Lamoury
J
J
Department of Neurology, Centre for Immunology, St Vincent's Hospital, Sydney, New South Wales, Australia. J.lamoury@cfi.unsw.edu.au
Williams
K R
KR
Lamoury
F M J
FM
Veas
L A
LA
Ajami
B
B
Taylor
R M
RM
Brew
B J
BJ
D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D006801:Humans; D007965:Leukodystrophy, Globoid Cell / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008648:Mesoderm / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008817:Mice, Mutant Strains; D039902:Multipotent Stem Cells / Q000378:metabolism* / Q000473:pathology; D009368:Neoplasm Transplantation / Q000379:methods; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D009836:Oligodendroglia / Q000378:metabolism / Q000473:pathology; D033581:Stem Cell Transplantation; D014183:Transplantation, Heterologous