Targeting and tracing antigens in live cells with fluorescent nanobodies.
We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.
Rothbauer
Ulrich
U
Ludwig Maximilians University Munich, Department of Biology II, Grosshaderner Str. 2, 82152 Planegg-Martinsried, Germany.
Zolghadr
Kourosh
K
Tillib
Sergei
S
Nowak
Danny
D
Schermelleh
Lothar
L
Gahl
Anja
A
Backmann
Natalija
N
Conrath
Katja
K
Muyldermans
Serge
S
Cardoso
M Cristina
MC
Leonhardt
Heinrich
H
D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D002162:Camelus / Q000276:immunology*; D000939:Epitopes; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000276:immunology; D008938:Mitosis; D016196:S Phase
D000906:Antibodies; D000941:Antigens; D000939:Epitopes; D007143:Immunoglobulin Heavy Chains; D049452:Green Fluorescent Proteins