title: Folate receptor-mediated cancer cell specific gene delivery using folic acid-conjugated oligochitosans.
abstract: Chitosan-mediated gene delivery has gained an increasing interest due to its ability to treat cancers and genetic diseases. However, low transfection efficiency and lack of target specificity limit its application for gene and drug delivery. In the present work, folic acid was covalently conjugated to chitosan as a targeting ligand in an attempt to specifically deliver DNA to folate receptor-overexpressing cancer cells. Folic acid-conjugated chitosan (FACN) was successfully synthesized and characterized by 1H-NMR and is biocompatible. In vitro gene transfer potential of FACN was evaluated in human epithelial ovarian cancer OV2008 cells and human breast cancer MCF-7 cells. FACN at a weight ratio of 10:1 exhibited significantly (< 0.01) enhanced gene transfer potential in folate receptor-overexpressing cancer cells as compared to unmodified chitosan. Transfection of FACN/pDNA nanocomplexes is competitively inhibited by free folic acid, suggesting the specific gene delivery of FACN/pDNA nanocomplexes is achieved through folate receptor-mediated endocytosis. Taken together, these results demonstrate that FACN provides a promising carrier for cancer gene therapy.
authors.affiliation: Department of Internal Medicine, Division of Allergy and Immunology, Joy McCann Culverhouse Airway Disease Center, University of South Florida, College of Medicine, James A. Haley Veteran's Hospital, Tampa, FI 33612, USA.
authors.forename: Dongwon
authors.initials: D
authors.lastname: Lee
authors.forename: Richard
authors.initials: R
authors.lastname: Lockey
authors.forename: Shyam
authors.initials: S
authors.lastname: Mohapatra
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D016503:Drug Delivery Systems / Q000379:methods*; D004705:Endocytosis; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000737:chemistry / Q000493:pharmacokinetics*; D018390:Gene Targeting / Q000379:methods; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D009369:Neoplasms / Q000503:physiopathology* / Q000628:therapy; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; D004247:DNA; D048271:Chitosan; D005492:Folic Acid