Tumor necrosis factor-alpha promoter polymorphisms in Mexican patients with spondyloarthritis.
To evaluate the role of tumor necrosis factor-alpha (TNF-alpha) gene as susceptibility marker for spondyloarthritis (SpA), two polymorphisms (-238 and -308 positions) were analyzed in 229 patients with SpA (113 with ankylosing spondylitis [AS], 92 with undifferentiated SpA [U-SpA], 24 with reactive arthritis), and 169 ethnically matched healthy control subjects. The HLA-B alleles were detected by PCR-SSP technique and the TNF-alpha polymorphism by PCR-RFLP. In comparison with healthy control subjects, the frequencies of TNF-238 in SpA were similar. In contrast, the analysis of -308 polymorphism showed increased frequencies of the T2(A) allele in the whole SpA group (p < 0.05, pC = NS, OR = 1.83) as well as the T2(A) allele (pC < 0.05, OR = 2.4) and T1T2(AG) genotype (p < 0.05, pC = NS, OR = 2.25) in U-SpA patients. Comparison of B27-negative patients and healthy control subjects yielded similar results. There was no significant correlation between TNF genotypes and clinical data. The present study demonstrates that TNF-alpha -308 polymorphism appears to be associated with the genetic susceptibility U-SpA. The association seems independent of the susceptibility conferred by the HLA-B27 in this group of patients.
Vargas-Alarcón
Gilberto
G
Department of Physiology, Instituto Nacional de Cardíología Ignacio Chávez, Mexico City, Mexico. gvargas63@yahoo.com
Casasola-Vargas
Julio
J
Rodríguez-Pérez
José Manuel
JM
Huerta-Sil
Gabriela
G
Pérez-Hernández
Nonanzit
N
Londoño
John
J
Pacheco-Tena
Cesar
C
Cardiel
Mario H
MH
Granados
Julio
J
Burgos-Vargas
Rubén
R
D000293:Adolescent; D000328:Adult; D016918:Arthritis, Reactive / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D015796:HLA-B27 Antigen / Q000235:genetics; D006801:Humans; D008297:Male; D008800:Mexico; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D025241:Spondylarthritis / Q000235:genetics*; D013167:Spondylitis, Ankylosing / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D015796:HLA-B27 Antigen; D014409:Tumor Necrosis Factor-alpha