Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection.
Protein arginine methyltransferase 1 (PRMT1) is currently thought as an effector to regulate interferon (IFN) signalling. Here Paralichthys olivaceus PRMT1 (PoPRMT1) gene was identified as a virally induced gene from UV-inactivated Scophthalmus maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). PoPMRT1 encodes a 341-amino-acid protein that shares the conserved domains including post-I, motif I, II and III. Homology comparisons show that the putative PoPMRT1 protein is the closest to zebrafish PMRT1 and belongs to type I PRMT family (including PRMT1, PRMT2, PRMT3, PRMT4, PRMT6, PRMT8). Expression analyses revealed an extensive distribution of PoPMRT1 in all tested tissues of flounder. In vitro induction of PoPRMT1 was determined in UV-inactivated SMRV-infected FEC cells, and under the same conditions, flounder Mx was also transcriptionally up-regulated, indicating that an IFN response might be triggered. Additionally, live SMRV infection of flounders induced an increased expression of PoPRMT1 mRNA and protein significantly in spleen, and to a lesser extent in head kidney and intestine. Immunofluorescence analysis revealed a major cyptoplasmic distribution of PoPRMT1 in normal FEC but an obvious increase occurred in nucleus in response to UV-inactivated SMRV. This is the first report on in vitro and in vivo expression of fish PRMT1 by virus infection, suggesting that PoPRMT1 might be implicated in flounder antiviral immune response.
Dong
Cai-Wen
CW
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Wuhan 430072, China.
Zhang
Yi-Bing
YB
Lu
Ai-Jun
AJ
Zhu
Rong
R
Zhang
Fu-Tie
FT
Zhang
Qi-Ya
QY
Gui
Jian-Fang
JF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000737:chemistry; D004625:Embryo, Nonmammalian / Q000166:cytology; D005393:Fish Diseases / Q000201:enzymology / Q000276:immunology* / Q000821:virology; D005432:Flounder / Q000235:genetics / Q000276:immunology* / Q000821:virology; D015971:Gene Expression Regulation, Enzymologic / Q000276:immunology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011484:Protein-Arginine N-Methyltransferases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012209:Rhabdoviridae / Q000276:immunology; D018353:Rhabdoviridae Infections / Q000201:enzymology / Q000276:immunology / Q000662:veterinary*; D016415:Sequence Alignment / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
D017931:DNA Primers; D012333:RNA, Messenger; D011484:Protein-Arginine N-Methyltransferases