title: Selective isolation of large chromosomal regions by transformation-associated recombination cloning for structural and functional analysis of mammalian genomes.
abstract: Transformation-associated recombination (TAR) cloning allows selective isolation of full-size genes and genomic loci as circular yeast artificial chromosomes in yeast. The method has a broad application for structural and functional genomics, long-range haplotyping, characterization of chromosomal rearrangements, and evolutionary studies. This chapter describes a basic protocol of gene isolation by TAR, as well as a method of conversion of TAR isolates into bacterial artificial chromosomes.
authors.affiliation: Laboratory of Biosystems and Cancer, National Cancer Institute, NIH, Bethesda, MD, USA.
authors.forename: Natalay
authors.initials: N
authors.lastname: Kouprina
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Noskov
authors.forename: Vladimir
authors.initials: V
authors.lastname: Larionov
mesh_terms: D000818:Animals; D022201:Chromosomes, Artificial / Q000235:genetics*; D003001:Cloning, Molecular; D016678:Genome / Q000235:genetics / Q000502:physiology*; D008322:Mammals / Q000235:genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012641:Selection, Genetic; D014170:Transformation, Genetic