Effect of nutritional status and ozone exposure on some biomarkers of oxidative stress in rat brain regions.
The aim of this study was to analyze the effect of nutritional condition and simulated exposure to ozone on Glutathione (GSH), the activity of Na+/K+ ATPase and lipid peroxidation in rat brain. Male Wistar rats were fed with 7% and 23% protein diets. Two groups were formed for each nutritional condition: one group was exposed for 15 successive days to 0.75 ppm of ozone and the other to air. Subsequently, the brain was dissected in cortex, hemispheres, cerebellum, and brainstem to measure the activity of thiobarbituric acid reactive substances (TBARS), ATPase, and levels of GSH. The activity of Na+/K+ ATPase increased in cerebellum of well-nourished rats exposed to ozone, while total ATPase and TBARS decreased in all studied areas in the malnourished groups. The levels of GSH decreased significantly (P < 0.05) in the brain of rats fed with 7% of protein diet and exposed to ozone but increased in rats fed with normal diet and exposed to ozone. These results suggest that malnutrition causes alterations in the values of Na+/K+ ATPase, total ATPase, GSH, and lipid peroxidation, while ozone contributes to these modifications. As a consequence, both variables are involved in oxidative stress in the rat brain.
Calderón Guzmán
David
D
Laboratorio de Neuroquímica, Instituto Nacional de Pediatría, Mexico Ciy, Mexico.
Barragán Mejía
Gerardo
G
Hernández García
Ernestina
E
Juárez Olguín
Hugo
H
D000818:Animals; D015415:Biomarkers; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009752:Nutritional Status / Q000502:physiology*; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D018384:Oxidative Stress; D010126:Ozone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D015415:Biomarkers; D010083:Oxidants, Photochemical; D017392:Thiobarbituric Acid Reactive Substances; D010126:Ozone; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione