title: Pro-inflammatory bone marrow milieu in patients with chronic idiopathic neutropenia is associated with impaired local production of interleukin-10.
abstract: The levels of interleukin-10 (IL-10) were evaluated in long-term bone marrow (BM) culture supernatants from 54 patients with chronic idiopathic neutropenia (CIN) and 30 healthy volunteers using enzyme-linked immunoabsorbent assay. Cytokine levels were significantly reduced in patients, compared with controls, and strongly correlated with peripheral blood neutrophil counts. Low levels of supernatant IL-10 were associated with increased values of supernatant IL-1beta, tumour necrosis factor-alpha, IL-6 and transforming growth factor-beta(1). We suggest that the pro-inflammatory milieu in the BM of CIN patients may be causatively related to the impaired production of IL-10, a cytokine normally displaying strong anti-inflammatory properties.
authors.affiliation: Department of Haematology, University of Crete School of Medicine, University Hospital of Heraklion, Crete, Greece.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Koumaki
authors.forename: Athina
authors.initials: A
authors.lastname: Damianaki
authors.forename: Maria
authors.initials: M
authors.lastname: Ximeri
authors.forename: Charis
authors.initials: C
authors.lastname: Pontikoglou
authors.forename: Fani
authors.initials: F
authors.lastname: Axioti
authors.forename: Michael
authors.initials: M
authors.lastname: Spanoudakis
authors.forename: George D
authors.initials: GD
authors.lastname: Eliopoulos
authors.forename: Helen A
authors.initials: HA
authors.lastname: Papadaki
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000276:immunology*; D002478:Cells, Cultured; D002908:Chronic Disease; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009503:Neutropenia / Q000276:immunology*; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D018836:Inflammation Mediators; D015850:Interleukin-6; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10