title: Influence of dietary calcium and phosphorus supply on epithelial phosphate transport in preruminant goats.
abstract: P homeostasis affected by high or low Ca and/or P supply in preruminant goats was characterized by balance studies in vivo. The main excretion pathway was the renal P(i) excretion whose extent was modulated by variations in dietary P and/or Ca supply. Faecal P excretion remained low irrespective of dietary regimen. The balance data were combined with respective in vitro data on P(i) transport properties and their adaptation in response to changes in dietary Ca and/or P intake. Therefore, P(i) transport capacities were determined by P(i) uptake into brush border membrane vesicles of jejunum and kidney. Epithelial P(i) transporters were determined semiquantitatively by northern and western blot analyses in jejunum, kidney and salivary gland. Renal P(i) transport was downregulated by doubling dietary P supply while doubling both, Ca and P as well as restrictive Ca at unchanged P led to slight, but not significant reductions in renal P(i) transport. Jejunal P(i) transport was reduced by P excess (doubling P and doubling both, Ca and P), but only NaPi IIb protein expression was significantly diminished. In conclusion, the significance of epithelial adaptation to dietary Ca and P supply for P homeostasis is discussed in preruminant goats.
authors.affiliation: Department of Physiology, School of Veterinary Medicine Hannover, Bischofsholer Damm 15/102, 30173, Hannover, Germany. korinna.huber@tiho-hannover.de
authors.forename: Korinna
authors.initials: K
authors.lastname: Huber
authors.forename: Uta
authors.initials: U
authors.lastname: Roesler
authors.forename: Antje
authors.initials: A
authors.lastname: Holthausen
authors.forename: Ernst
authors.initials: E
authors.lastname: Pfeffer
authors.forename: Gerhard
authors.initials: G
authors.lastname: Breves
mesh_terms: D000821:Animal Feed; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002117:Calcitriol / Q000097:blood; D002118:Calcium / Q000378:metabolism; D002136:Calcium, Dietary / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006041:Goats / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D007583:Jejunum / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008297:Male; D010710:Phosphates / Q000378:metabolism*; D016226:Phosphorus, Dietary / Q000494:pharmacology*; D012417:Rumen / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism; D050610:Sodium-Phosphate Cotransporter Proteins, Type II / Q000235:genetics / Q000378:metabolism
chemical_list: D002136:Calcium, Dietary; D010710:Phosphates; D016226:Phosphorus, Dietary; D050610:Sodium-Phosphate Cotransporter Proteins, Type II; D002117:Calcitriol; D002118:Calcium