title: Determination of the profile of fatty acids of 4 species of Shigella spp by chromatography of gases.
abstract: Shigella boydii, Shigella flexneri, Shigella dysenteriae and Shigella sonnei were identified using gas chromatography instead of the traditional techniques. Their acid methyl esters profiles were determined using a gas chromatograph Hewlett Packard 5890A and a RSL-150 heliflex capillary column. A total of 192 samples were analyzed both reference strains (ATCC 8700, INDRE B2188, B2194 and B2199) and environmental isolates. 12 fatty acids were included in the profiles from which 3-hydroxytetradecanoic acid (peak 12), trans 9-octadecanoic acid (peak 22), heptadecanoic acid (peak 18) and octadecanoic acid (peak 23), were the most important for the differentiation of the species analyzed.
authors.affiliation: Unidad de Investigación Interdisciplinaria en Ciencias de la Salud y la Educación, UNAM FES Iztacala, México. erobles@servidor.unam.mx
authors.forename: E
authors.initials: E
authors.lastname: Robles Valderrama
authors.forename: M D
authors.initials: MD
authors.lastname: Hurtado Bocanegra
authors.forename: M E
authors.initials: ME
authors.lastname: González Arreaga
authors.forename: P
authors.initials: P
authors.lastname: Ramírez García
authors.forename: A
authors.initials: A
authors.lastname: Durán Díaz
authors.forename: R
authors.initials: R
authors.lastname: Ayala Patiño
authors.forename: M E
authors.initials: ME
authors.lastname: Martínez Pérez
mesh_terms: D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D012760:Shigella / Q000737:chemistry*
chemical_list: D005227:Fatty Acids