title: [Association of abnormal cyclooxygenase-2 gene expression with colorectal carcinoma metastasis].
abstract: OBJECTIVE
  To investigate cyclooxygenase-2 (COX-2) expression of in colorectal carcinoma cell lines and tissues and its clinical implications.
  
  
  METHODS
  SP immunohistochemistry was used to detect COX-2 protein in SW480 and SW620 cell lines and 50 primary colorectal carcinoma and 50 lymphoma metastasis carcinoma specimens. Real-time PCR was used to detect COX-2 mRNA expression in SW480 and SW620 cell lines.
  
  
  RESULTS
  SW480 and SW620 cell lines both highly expressed COX-2. The positive expression levels of COX-2 increased significantly in lymph node metastatic carcinoma in comparison with primary colorectal carcinoma (P<0.05). The expression COX-2 mRNA in SW620 cell line was higher than that of SW480 cell line, showing a mean expression increment of 2.268 folds in SW620 cell line relative to SW480.
  
  
  CONCLUSIONS
  Elevated COX-2 expression may be associated with lymph node metastases of colorectal carcinoma.
authors.affiliation: Key Laboratory of Functional Proteomics and Transcriptomics of Main Human Diseases of Guangdong Province and Ministry of Education, Department of Pathology, Nangfang Hospital, Southern Medical University, Guangzhou 510515, China. Lizg@fimmu.com
authors.forename: Zu-guo
authors.initials: ZG
authors.lastname: Li
authors.forename: Teng-fei
authors.initials: TF
authors.lastname: Liu
authors.forename: Wei-bing
authors.initials: WB
authors.lastname: Xie
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Yan-qing
authors.initials: YQ
authors.lastname: Ding
mesh_terms: D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D051546:Cyclooxygenase 2