Cardiac muscle sarcolemma chromatographic stationary phase and its potential application in drug screening.
A new bioactive packing material for liquid chromatography, sarcolemma chromatography stationary phase (SCSP), is presented. Its surface characteristics are investigated, and it is found that the acceptors embedded in sarcolemma remained bioactive for more than a week. The retention behavior of antagonists and activators related to cardiac muscle sarcolemma on the SCSP chromatographic column shows the screening function of the SCSP column, and the retention behavior of the active components in the aether extract from the Chinese herb Ligusticum chuanxiong Hort. on the SCSP column reveals, to a certain extent, the separation function of the SCSP column. These suggest that SCSP is a potentially useful material in drug screening.
Zhang
Zhi-Qi
ZQ
School of Chemistry and Material Science, Shaanxi Normal University, Xi'an, 710062, P.R. China. zqzhang@snnu.edu.cn
Yue
Xuan-Feng
XF
Zhang
Yan-Ni
YN
Tian
Zhen-Jun
ZJ
Yang
Jian-Xiong
JX
D030004:Ligusticum / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D009206:Myocardium / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D010936:Plant Extracts / Q000032:analysis; D012508:Sarcolemma / Q000737:chemistry*
D004364:Pharmaceutical Preparations; D010936:Plant Extracts