[Study on time-effect and dose-effect of serum containing Chinese herbal medicine].
OBJECTIVE
To study the time-effect, dose-effect of serum containing Shuangshentongguan (SSTG), a formula composed by active fraction of Chinese medicine.


METHODS
Serum containing SSTG was obtained at different time after perorally administrated with different dose of SSTG. The cardiomyocytes were deprived of oxygen and glucose to mimic hypoxia reoxygenation injury and were treated by serum containing SSTG when reoxygenation. LDH content in supernatant was detected after reoxygenation. LDH release suppression rate was used to study the time-effect and dose-effect of serum containing SSTG.


RESULTS
The suppression rate of LDH release was not satisfied in serum containing high dose of SSTG. There was no significant dose-effect relationship between them. The suppression rate of LDH release was also different in drug serum taken at different time after SSTG treatment.


CONCLUSIONS
It was not the case that the higher the dose given to animal, the better the pharmacological effects.
Liu
Jian-xun
JX
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. jianxun@ht.rol.cn.net
Han
Xiao
X
D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D031311:Corydalis / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry; D044967:Serum; D013995:Time
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007770:L-Lactate Dehydrogenase