title: Kinetics of in vitro lysozyme deposition on silicone hydrogel, PMMA, and FDA groups I, II, and IV contact lens materials.
abstract: We sought to compare the kinetics of in vitro lysozyme deposition on silicone hydrogel (SH), polymethyl methacrylate (PMMA), and FDA groups I, II, and IV contact lenses. Lenses were incubated in 125I-labeled lysozyme for time periods ranging from 1 hr to 28 days, and radioactive counts were determined. SH lenses and PMMA deposited less lysozyme than conventional hydrogel lenses (p < 0.05). Lysozyme accumulation on group IV lenses reached a maximum on the seventh day and then plateaued, whereas on groups I, II, and SH lenses, deposition continued to increase across all time periods, reiterating that kinetics of lysozyme deposition is highly material dependent.
authors.affiliation: Centre for Contact Lens Research, School of Optometry, University of Waterloo, Waterloo, Ontario, Canada. lnagapat@uwaterloo.ca
authors.forename: Lakshman N
authors.initials: LN
authors.lastname: Subbaraman
authors.forename: Mary-Ann
authors.initials: MA
authors.lastname: Glasier
authors.forename: Michelle
authors.initials: M
authors.lastname: Senchyna
authors.forename: Heather
authors.initials: H
authors.lastname: Sheardown
authors.forename: Lyndon
authors.initials: L
authors.lastname: Jones
mesh_terms: D001672:Biocompatible Materials; D003261:Contact Lenses; D018795:Device Approval; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D009113:Muramidase / Q000378:metabolism*; D019904:Polymethyl Methacrylate / Q000378:metabolism*; D011485:Protein Binding; D012826:Silicone Elastomers / Q000378:metabolism*; D014481:United States; D014486:United States Food and Drug Administration
chemical_list: D001672:Biocompatible Materials; D012826:Silicone Elastomers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D019904:Polymethyl Methacrylate; D009113:Muramidase