Expression profiling of mouse placental lactogen II and its correlative genes using a cDNA microarray analysis in the developmental mouse placenta.
The placenta is a highly differentiated organ essential for embryonic growth and development. In order to search for key molecules that are associated with mouse placental lactogen II (mPL-II) gene expression, we applied mouse cDNA microarray analysis to RNAs extracted from placentae on days 10, 12, 14, 16 and 18 of pregnancy. Changes in gene expression were categorized between days 10 and 12, 12 and 14, 14 and 16 and 16 and 18 of pregnancy. After microarray analysis, which had a minimum detectable fold change for differential expression of 2, we selected 10 genes, Apoa2, Apoc2, Ceacam14, Creg1, Fmo1, Igf2, Slc2a1, Spink3, Spi1-1 and Tpbpa, exhibiting a expression pattern similar to the mPL-II gene. Furthermore, we performed real-time PCR analysis and in situ hybridization (ISH) to find correlative expression genes for the mPL-II gene. From these results, we identified a resemblance in gene expression between mPL-II and Igf2 and selected these genes for performance of double-fluorescence immunohistochemical staining. We colocalized these proteins in labyrinthine trophoblast cells. These results strongly suggest that the expression of mPL-II and Igf2 is highly related to placental development in mice. This large-scale identification of genes regulated during placentogenesis assists in further elucidation of the molecular basis of extraembryonic development and function.
Ishida
Michiyo
M
Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji University, Japan. michi@isc.meiji.ac.jp
Ohashi
Satoshi
S
Kizaki
Yusuke
Y
Naito
Jyun-ichiro
J
Horiguchi
Kotaro
K
Harigaya
Toshio
T
D000818:Animals; D016633:Apolipoprotein A-II / Q000235:genetics; D053304:Apolipoprotein C-II / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D051272:Glucose Transporter Type 1 / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics; D007335:Insulin-Like Growth Factor II / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D018919:Neovascularization, Physiologic / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D010105:Oxygenases / Q000235:genetics; D010920:Placenta / Q000098:blood supply / Q000502:physiology*; D010928:Placental Lactogen / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D029584:Prostatic Secretory Proteins / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D014359:Trypsin Inhibitor, Kazal Pancreatic; D000099232:Proto-Oncogene Protein Spi-1
D016633:Apolipoprotein A-II; D053304:Apolipoprotein C-II; D015815:Cell Adhesion Molecules; D051272:Glucose Transporter Type 1; D006023:Glycoproteins; D007335:Insulin-Like Growth Factor II; D010105:Oxygenases; D010928:Placental Lactogen; D029584:Prostatic Secretory Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D015534:Trans-Activators; D014359:Trypsin Inhibitor, Kazal Pancreatic; D000099232:Proto-Oncogene Protein Spi-1; C513121:IGF2 protein, mouse; C495678:Slc2a1 protein, mouse; C487134:Spink1 protein, mouse; C079456:amyloidogenic apolipoprotein A-II, mouse; C020066:dimethylaniline monooxygenase (N-oxide forming)