title: [Determination of salvianolic acid B and paeoniflorin in Shuangdan granules by HPLC].
abstract: OBJECTIVE
  To develop a method for the determination of salvianolic acid B and paeoniflorin in Shuangdan granules.
  
  
  METHODS
  The chromatographic separation was performed on a Zorbax SB C18 column with a linear gradient elution of phosphoric acid-water (0.05:100) and phosphoric acid-acetonitrile-methanol (0.05:4:96) was used. The flow rate was 1 mL x min(-1) and column temperature was set at 25 degrees C. The UV detection wavelength was set at 230 nm.
  
  
  RESULTS
  The linear ranger was of 0.710-22.7 microg for salvianolic acid B and 0.103-3.30 microg for paeoniflorin. Three batches of Shuangdan granules were analyzed, and the content of salvianolic acid B ranged from 6.46-11.6 mg x g(-1) and paeoniflorin ranged from 1.44-1.78 mg x g(-1).
  
  
  CONCLUSIONS
  The method was accurate, reproducible, reliable, and could be used for quantitative control of Shuangdan granules.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Ge
authors.forename: Qing
authors.initials: Q
authors.lastname: He
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Shui
authors.forename: Yi-yu
authors.initials: YY
authors.lastname: Cheng
mesh_terms: D001565:Benzoates / Q000032:analysis*; D001572:Benzofurans / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D005960:Glucosides / Q000032:analysis*; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry; D006027:Glycosides; D053630:Depsides
chemical_list: D001565:Benzoates; D001572:Benzofurans; D001952:Bridged-Ring Compounds; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C521472:Shuangdan; C015423:peoniflorin; D006027:Glycosides; C076944:salvianolic acid B; D053630:Depsides