title: Specificity of DC-SIGN for mannose- and fucose-containing glycans.
abstract: The dendritic cell specific C-type lectin dendritic cell specific ICAM-3 grabbing non-integrin (DC-SIGN) binds to "self" glycan ligands found on human cells and to "foreign" glycans of bacterial or parasitic pathogens. Here, we investigated the binding properties of DC-SIGN to a large array of potential ligands in a glycan array format. Our data indicate that DC-SIGN binds with K(d)<2muM to a neoglycoconjugate in which Galbeta1-4(Fucalpha1-3)GlcNAc (Le(x)) trisaccharides are expressed multivalently. A lower selective binding was observed to oligomannose-type N-glycans, diantennary N-glycans expressing Le(x) and GalNAcbeta1-4(Fucalpha1-3)GlcNAc (LacdiNAc-fucose), whereas no binding was observed to N-glycans expressing core-fucose linked either alpha1-6 or alpha1-3 to the Asn-linked GlcNAc of N-glycans. These results demonstrate that DC-SIGN is selective in its recognition of specific types of fucosylated glycans and subsets of oligomannose- and complex-type N-glycans.
authors.affiliation: Department of Molecular Cell Biology and Immunology, VU University Medical Center, Van der Boechorstsraat 7, 1081 BT Amsterdam, The Netherlands.
authors.forename: Ellis
authors.initials: E
authors.lastname: van Liempt
authors.forename: Christine M C
authors.initials: CM
authors.lastname: Bank
authors.forename: Padmaja
authors.initials: P
authors.lastname: Mehta
authors.forename: Juan Jesús
authors.initials: JJ
authors.lastname: Garciá-Vallejo
authors.forename: Ziad S
authors.initials: ZS
authors.lastname: Kawar
authors.forename: Rudolf
authors.initials: R
authors.lastname: Geyer
authors.forename: Richard A
authors.initials: RA
authors.lastname: Alvarez
authors.forename: Richard D
authors.initials: RD
authors.lastname: Cummings
authors.forename: Yvette van
authors.initials: Yv
authors.lastname: Kooyk
authors.forename: Irma
authors.initials: I
authors.lastname: van Die
mesh_terms: D015815:Cell Adhesion Molecules / Q000378:metabolism*; D005643:Fucose; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D008024:Ligands; D008358:Mannose; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D040081:Protein Array Analysis; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D005643:Fucose; D037181:Lectins, C-Type; D008024:Ligands; D008358:Mannose; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin