title: Gene expression of leucocytes in vaccinated Japanese flounder (Paralichthys olivaceus) during the course of experimental infection with Edwardsiella tarda.
abstract: In this paper, we focused on the detection of differentially expressed genes in peripheral blood leucocytes (PBL) during the course of Edwardsiella tarda infection in vaccinated and non-vaccinated Japanese flounder (Paralichthys olivaceus). cDNA microarray analysis was performed to compare the gene expression patterns of the PBL between the vaccinated and non-vaccinated fish in response to E. tarda inoculation. Fish were vaccinated twice, at a two-week interval and experimentally challenged with E. tarda two weeks after the second vaccination. Among the 1187 analyzed genes, 42 genes were up-regulated during the course of infection either in vaccinated or non-vaccinated fish. These genes included immune-related genes, such as MMP-9, MMP-13, CXC chemokine, CD20 receptor and hepcidin. Some immune-related genes were down-regulated after the E. tarda challenge, i.e. interferon inducible Mx protein, MHC class II-associated invariant chain, MHC class II alpha and MHC class II beta encoding genes, immunoglobulin light chain precursor, immunoglobulin light chain and IgM. These responses are thought to be a common reaction of Japanese flounder PBL in the course of edwardsiellosis, irrespective of immunized condition. Ten genes were significantly up-regulated only in vaccinated fish, and 11 genes were significantly up-regulated only in non-vaccinated fish. These genes may have a correlation with the efficacy of vaccination, although we have no evidence to link the different gene expression patterns and the efficacy of vaccination at present.
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, Nansei, Mie 516-0193, Japan. matsuym@fra.affrc.go.jp
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Matsuyama
authors.forename: Atushi
authors.initials: A
authors.lastname: Fujiwara
authors.forename: Chihaya
authors.initials: C
authors.lastname: Nakayasu
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamaishi
authors.forename: Norihisa
authors.initials: N
authors.lastname: Oseko
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D020609:Edwardsiella tarda / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D005432:Flounder / Q000276:immunology* / Q000382:microbiology; D005786:Gene Expression Regulation; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary
chemical_list: D001428:Bacterial Vaccines