title: [Fingerprints of Rhizoma Coptidis from Shizhu by HPLC].
abstract: OBJECTIVE
  To establish a sensitive and specific HPLC method for the quality control of Rhizoma Coptidis collected from shizhu in chongqing.
  
  
  METHODS
  The HPLC fingerprints of Rhizoma Coptidis from shizhu were obtained from Waters instrument. The methods was performed on a Diamonsil C18 column gradient eluted with acetonitrile-0.05 mol/L KH2PO4 (pH3 with H3PO4) at the flow rate of 0.8 m/min. The temperature of column was 25 degrees C and the UV detection wavelength was 270nm.
  
  
  RESULTS
  The HPLC fingerprint of Rhizoma Coptis, showing 16 characteristic peaks, was established from 10 lots of Rhizoma Coptis. By comparision of the retention time and the on-line UV spectra of chemical standards, peak 11, 12, 13, 14 and 15 were identified as Epiberberine, Jatrorrhizine, Coptisine, Palmatine and Berberine.
  
  
  CONCLUSIONS
  The HPLC fingerprint of Rhizoma Coptidis with high specificity can be used to control its quality.
authors.affiliation: Chongqing Academy of Chinese Materia Medica, China.
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Huang
authors.forename: Long-yun
authors.initials: LY
authors.lastname: Li
authors.forename: Xian-you
authors.initials: XY
authors.lastname: Qu
authors.forename: Guang-lin
authors.initials: GL
authors.lastname: Cui
mesh_terms: D001599:Berberine / Q000031:analogs & derivatives* / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D039323:Coptis / Q000737:chemistry* / Q000145:classification; D004355:Drug Stability; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry
chemical_list: D001600:Berberine Alkaloids; D001599:Berberine