Evidence of modified nuclear protein matrix in human spermatozoa with fragmented deoxyribonucleic acid.
Human spermatozoa were processed for determination of DNA fragmentation with use of an in situ diffusion assay, so that those cells containing DNA fragmentation produce extensive peripheral dissemination of DNA fragments after lysis in an agarose microgel. Quantification of specific protein staining confirmed that sperm cells without DNA fragmentation had almost complete removal of nuclear matrix proteins, whereas spermatozoa with DNA fragmentation tended to retain residual nucleoskeletal protein in a collapsed and condensed state. This result suggests that a modified nuclear protein matrix associates with fragmented sperm DNA.
Santiso
Rebeca
R
Sección de Genética y Unidad de Investigación, Complejo Hospitalario Universitario Juan Canalejo, A Coruña, Spain.
Muriel
Lourdes
L
Goyanes
Vicente
V
Segrelles
Enrique
E
Gosálvez
Jaime
J
Fernández
José Luis
JL
D002478:Cells, Cultured; D053938:DNA Fragmentation; D019317:Evidence-Based Medicine; D006801:Humans; D008297:Male; D015530:Nuclear Matrix / Q000235:genetics* / Q000378:metabolism*; D034521:Nuclear Matrix-Associated Proteins / Q000235:genetics* / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*
D034521:Nuclear Matrix-Associated Proteins