title: Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription.
abstract: The mRNA nuclear export receptor Mex67/Mtr2 is recruited to mRNAs through RNA-binding adaptors, including components of the THO/TREX complex that couple transcription to mRNA export. Here we show that the ubiquitin-associated (UBA) domain of Mex67 is not only required for proper nuclear export of mRNA but also contributes to recruitment of Mex67 to transcribing genes. Our results reveal that the UBA domain of Mex67 directly interacts with polyubiquitin chains and with Hpr1, a component of the THO/TREX complex, which is regulated by ubiquitylation in a transcription-dependent manner. This interaction transiently protects Hpr1 from ubiquitin/proteasome-mediated degradation and thereby coordinates recruitment of the mRNA export machinery with transcription and early messenger ribonucleoproteins assembly.
authors.affiliation: Institut Jacques Monod, Unité Mixte de Recherche 7592, Centre National de la Recherche Scientifique, Universités Paris VI and VII, 2 Place Jussieu, Tour 43, 75251 Paris Cedex 05, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Gwizdek
authors.forename: Nahid
authors.initials: N
authors.lastname: Iglesias
authors.forename: Manuel S
authors.initials: MS
authors.lastname: Rodriguez
authors.forename: Batool
authors.initials: B
authors.lastname: Ossareh-Nazari
authors.forename: Maria
authors.initials: M
authors.lastname: Hobeika
authors.forename: Gilles
authors.initials: G
authors.lastname: Divita
authors.forename: Françoise
authors.initials: F
authors.lastname: Stutz
authors.forename: Catherine
authors.initials: C
authors.lastname: Dargemont
mesh_terms: D001692:Biological Transport; D004268:DNA-Binding Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C063494:HPR1 protein, S cerevisiae; C106898:MEX67 protein, S cerevisiae; D009687:Nuclear Proteins; D029543:Nucleocytoplasmic Transport Proteins; C490846:RAD23 protein, S cerevisiae; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex