title: Comparison of multiple genes of spring viremia of carp viruses isolated in the United States.
abstract: Five spring viremia of carp viruses (SVCV), Rhabdovirus carpio, were isolated in the United States (US) between 2002 and 2004. Single tube reverse transcription-polymerase chain reaction (RT-PCR) was used to generate overlapping cDNA fragments from the US isolates of SVCV. Multiple pairs of specific primers were designed to amplify a portion of the phosphoprotein gene, the matrix gene, and the glycoprotein gene of SVCV genogroup Id (corresponding to nucleotides 2174-4942 of GenBank accession NC_002803). Sequences were proofread and aligned to generate a consensus sequence for each isolate. Phylogenetic analysis of the 2705 nucleotide consensus sequence revealed that all five US isolates belong to SVCV genogroup Ia, Asian origin isolates, and a PCR primer binding site unique to SVCV genogroup Ia was identified.
authors.affiliation: Diagnostic Virology Laboratory, National Veterinary Services Laboratories, VS-APHIS-USDA, Ames, IA 50010, USA. Janet.V.Warg@aphis.usda.gov
authors.forename: Janet V
authors.initials: JV
authors.lastname: Warg
authors.forename: Audrey L
authors.initials: AL
authors.lastname: Dikkeboom
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Goodwin
authors.forename: Kevin
authors.initials: K
authors.lastname: Snekvik
authors.forename: John
authors.initials: J
authors.lastname: Whitney
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000821:virology*; D002478:Cells, Cultured; D005393:Fish Diseases / Q000821:virology*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012209:Rhabdoviridae / Q000235:genetics* / Q000302:isolation & purification*; D018353:Rhabdoviridae Infections / Q000821:virology*; D012621:Seasons; D017385:Sequence Homology; D014481:United States; D014766:Viremia / Q000821:virology*