title: Changes in the mRNA transcripts of insulin-like growth factor ligand, receptors and binding proteins in bovine blastocysts and elongated embryos derived from somatic cell nuclear transfer.
abstract: The objective of this study was to determine changes in the transcription of insulin-like growth factor (IGF)-related genes in blastocyst (BC)- and elongated (EL)-stage embryos produced by nuclear transfer using somatic cells (NT-SC). Bovine BC (day 7)- and EL (day 15)-stage embryos were obtained from NT-SC or in vivo production (Vivo). The relative abundance of mRNA was examined by RT- real-time PCR. The transcript of IGF-II was only detected at the EL stage in both the NT-SC and Vivo embryos. The level of transcription of the IGF-I receptor (r) in the NT-SC embryos was decreased at the EL stage and was significantly (P<0.05) lower than at the BC stage. In contrast, the IGF-IIr levels did not differ significantly between the NT-SC and Vivo embryos, regardless of the developmental stage. IGF-binding protein (IGFBP)-2 levels were markedly decreased in the NT-SC and Vivo embryos at the EL stage (P<0.05). The IGFBP-3 level in Vivo was significantly (P<0.05) increased at the EL stage compared with at the BC stage. However, the IGFBP-3 levels in NT-SC embryos were unchanged and lower (P<0.05) than in the Vivo embryos at the EL stage. These results suggest that there are differences in the levels and changes in the transcription of IGF-related genes in bovine embryos produced by NT-SC compared with those produced by Vivo.
authors.affiliation: Department of Animal Biotechnology, Hokkaido Animal Research Center, Japan. kensawai@agri.pref.hokkaido.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Sawai
authors.forename: Soichi
authors.initials: S
authors.lastname: Kageyama
authors.forename: Satoru
authors.initials: S
authors.lastname: Moriyasu
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hirayama
authors.forename: Akira
authors.initials: A
authors.lastname: Minamihashi
authors.forename: Sadao
authors.initials: S
authors.lastname: Onoe
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002417:Cattle; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000235:genetics / Q000378:metabolism; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000235:genetics / Q000378:metabolism; D018969:Insulin-Like Growth Factor Binding Proteins / Q000235:genetics* / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000235:genetics / Q000378:metabolism; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D017526:Receptor, IGF Type 1 / Q000235:genetics / Q000378:metabolism; D017527:Receptor, IGF Type 2 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: D018971:Insulin-Like Growth Factor Binding Protein 2; D018972:Insulin-Like Growth Factor Binding Protein 3; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D017527:Receptor, IGF Type 2; D007335:Insulin-Like Growth Factor II; D017526:Receptor, IGF Type 1