title: Boric acid and boronic acids inhibition of pigeonpea urease.
abstract: Urease from the seeds of pigeonpea was competitively inhibited by boric acid, butylboronic acid, phenylboronic acid, and 4-bromophenylboronic acid; 4-bromophenylboronic acid being the strongest inhibitor, followed by boric acid > butylboronic acid > phenylboronic acid, respectively. Urease inhibition by boric acid is maximal at acidic pH (5.0) and minimal at alkaline pH (10.0), i.e., the trigonal planar B(OH)3 form is a more effective inhibitor than the tetrahedral B(OH)4 -anionic form. Similarly, the anionic form of phenylboronic acid was least inhibiting in nature.
authors.affiliation: School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: K Ravi Charan
authors.initials: KR
authors.lastname: Reddy
authors.forename: Arvind M
authors.initials: AM
authors.lastname: Kayastha
mesh_terms: D001888:Boric Acids / Q000737:chemistry*; D001897:Boronic Acids / Q000737:chemistry*; D036561:Cajanus / Q000201:enzymology*; D002384:Catalysis; D015195:Drug Design; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D010936:Plant Extracts / Q000378:metabolism; D014508:Urea / Q000737:chemistry; D014510:Urease / Q000037:antagonists & inhibitors* / Q000737:chemistry*
chemical_list: D001888:Boric Acids; D001897:Boronic Acids; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D014508:Urea; D014510:Urease; C032688:boric acid