title: Dual-labeled telomere sensing probes for quantification of telomerase activity assay.
abstract: The present study describes an empirically discovered phenomenon that might be useful for development of a sensitive and rapid methodology for quantification of telomerase activity assay with simple data acquisition and possibility for calculation of telomerase product in absolute units. The method is based on the design and application of two single-stranded telomere sensing probes consisting of dual-labeled 16-mer oligonucleotides (fluorescent Cy3/Cy3-labeled and non-fluorescent IowaBlack/BHQ-labeled) that can simultaneously hybridize on the primary product of the telomerase reaction.
authors.affiliation: On-site Sensing and Diagnosis Research Laboratory, AIST-Kyushu, 807-1 Shuku-machi, Tosu 841-0052, Japan. r.bakalova-zheleva@aist.go.jp
authors.forename: Rumiana
authors.initials: R
authors.lastname: Bakalova
authors.forename: Zhivko
authors.initials: Z
authors.lastname: Zhelev
authors.forename: Takanori
authors.initials: T
authors.lastname: Kubo
authors.forename: Milka
authors.initials: M
authors.lastname: Mileva
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohba
mesh_terms: D001483:Base Sequence; D002232:Carbocyanines; D015342:DNA Probes / Q000235:genetics; D005456:Fluorescent Dyes; D015336:Molecular Probe Techniques; D019098:Telomerase / Q000032:analysis*; D016615:Telomere / Q000201:enzymology* / Q000235:genetics
chemical_list: D002232:Carbocyanines; D015342:DNA Probes; D005456:Fluorescent Dyes; C089187:cyanine dye 3; D019098:Telomerase