Biotransformation of Hg(II) by cyanobacteria.
The biotransformation of Hg(II) by cyanobacteria was investigated under aerobic and pH-controlled culture conditions. Mercury was supplied as HgCl(2) in amounts emulating those found under heavily impacted environmental conditions where bioremediation would be appropriate. The analytical procedures used to measure mercury within the culture solution, including that in the cyanobacterial cells, used reduction under both acid and alkaline conditions in the presence of SnCl(2). Acid reduction detected free Hg(II) ions and its complexes, whereas alkaline reduction revealed that meta-cinnabar (beta-HgS) constituted the major biotransformed and cellularly associated mercury pool. This was true for all investigated species of cyanobacteria: Limnothrix planctonica (Lemm.), Synechococcus leopoldiensis (Racib.) Komarek, and Phormidium limnetica (Lemm.). From the outset of mercury exposure, there was rapid synthesis of beta-HgS and Hg(0); however, the production rate for the latter decreased quickly. Inhibitory studies using dimethylfumarate and iodoacetamide to modify intra- and extracellular thiols, respectively, revealed that the former thiol pool was required for the conversion of Hg(II) into beta-HgS. In addition, increasing the temperature enhanced the amount of beta-HgS produced, with a concomitant decrease in Hg(0) volatilization. These findings suggest that in the environment, cyanobacteria at the air-water interface could act to convert substantial amounts of Hg(II) into beta-HgS. Furthermore, the efficiency of conversion into beta-HgS by cyanobacteria may lead to the development of applications in the bioremediation of mercury.
Lefebvre
Daniel D
DD
Department of Biology, Queen's University, Kingston, ON K7L 3N6, Canada. lefebvre@biology.queensu.ca
Kelly
David
D
Budd
Kenneth
K
D001673:Biodegradation, Environmental; D000458:Cyanobacteria / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008627:Mercuric Chloride / Q000378:metabolism*; D008628:Mercury / Q000378:metabolism*; D017669:Mercury Compounds / Q000378:metabolism*; D031654:Nuphar / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D012989:Soil Pollutants / Q000378:metabolism; D046940:Synechococcus / Q000378:metabolism; D014873:Water Pollutants / Q000378:metabolism
D017669:Mercury Compounds; D012989:Soil Pollutants; D014873:Water Pollutants; D008627:Mercuric Chloride; D008628:Mercury; C034211:cinnabar