Naturally occurring antisense RNA of allatostatin gene in the prawn, Macrobrachium rosenbergii.
Allatostatins are important regulatory neuropeptides which are widely distributed in invertebrates and execute their functions through either neural or humoral routes. However, the regulatory mechanism of the gene expression is unclear. In this paper, we report a naturally occurring antisense transcript, named as asMacro-AST A, of the crustacean FGLamide allatostatin gene (Macro-AST A) from the prawn, Macrobrachium rosenbergii. The asMacro-AST A contains an 843-bp sequence fully complementary to the 3' end of the Macro-AST A. To our knowledge, this is the first report of a natural antisense transcript in crustacean and the first endogenous antisense transcript of all identified allatostatin genes. Northern blotting analysis demonstrated that the gene was expressed in the thoracic ganglia where the sense gene was also expressed. Furthermore, we have detected a RNA-RNA duplex between the sense-antisense complementary region by using RNase protection analysis and RT-PCR. These results suggest that the antisense gene may play a role in the regulation of Macro-AST A gene expression.
Yin
Guo-Li
GL
Institute of Cell Biology and Genetics, College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou, Zhejiang, 310058 P. R. China.
Chen
Qi
Q
Yang
Wei-Jun
WJ
D000818:Animals; D033364:Decapoda / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology*; D009479:Neuropeptides / Q000235:genetics*; D016372:RNA, Antisense / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D009479:Neuropeptides; D016372:RNA, Antisense; C076523:allatostatin