(S)-Ibuprofen-imprinted polymers incorporating gamma-methacryloxypropyl-trimethoxysilane for CEC separation of ibuprofen enantiomers.
In this report, a novel preparation method of molecularly imprinted polymers (MIPs) for CEC was developed. Molecularly imprinted monolithic columns for (S)-ibuprofen were prepared and evaluated, in which charged entities responsible for establishing EOF have been derived from gamma-methacryloxypropyltrimethoxysilane (gamma-MAPS), which was hydrolyzed following copolymerization with 4-vinylpyridine (4-VPY) and ethylene glycol dimethacrylate (EDMA). The EOF and molecular recognition of the stationary phase were investigated in aqueous and nonaqueous media, respectively. The experimental results indicated that the material showed a reasonably stable EOF and the prepared separation materials were capable of separating racemic ibuprofen, a task that could not be accomplished by MIPs prepared in parallel, using methacrylic acid (MAA) as a functional monomer. The efficiency at pH 3.2 for the first-eluted enantiomer and the last-eluted enantiomer (the imprinted analyte) were 128,700 and 2100 plates/m, respectively.
Deng
Qi-Liang
QL
College of Science, Tianjin University of Science and Technology, Tianjin, China.
Lun
Zhi-Hong
ZH
Gao
Ru-Yu
RY
Zhang
Li-Hua
LH
Zhang
Wei-Bing
WB
Zhang
Yu-Kui
YK
D000179:Acrylates / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007052:Ibuprofen / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D008689:Methacrylates / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D012821:Silanes / Q000737:chemistry*; D013237:Stereoisomerism
D000179:Acrylates; D008689:Methacrylates; D011108:Polymers; D011725:Pyridines; D012821:Silanes; C017492:methacryloxypropyltrimethoxysilane; C004919:ethylene dimethacrylate; C029351:4-vinylpyridine; C035956:methyl acrylate; D007052:Ibuprofen