The gene responsible for borate cross-linking of pectin Rhamnogalacturonan-II is required for plant reproductive tissue development and fertilization.
Deficiencies in boron, a microelement that is essential for the growth and development of higher plants, often cause problems in reproductive growth. Rhamnogalacturonan-II (RG-II) in cell wall pectin acts as the sole receptor for boron in plant cells, forming a borate cross-linked RG-II dimer (dRG-II-B), but the physiological functions of dRG-II-B remain unknown. We have previously shown that the pectin glucuronyltransferase 1 gene NpGUT1, which is involved in the biosynthesis of RG-II sugar chains, is essential for the formation of the RG-II-B complex, resulting in tight intercellular attachment in meristematic tissues. Because NpGUT1 expression was found to be abundant in reproductive organs in addition to meristematic tissues, we analyzed the expression and functions of NpGUT1 in more detail in tobacco reproductive tissues. Specific NpGUT1 expression was detected in the tapetum of flower buds and in the pollen, pollen tube tips, and transmitting tissue of the pistils of flowers. Dexamethasone-induced expression of the NpGUT1 antisense gene in flower buds resulted in the formation of sterile flowers with aberrant development of pollen and transmitting tissue. Pollen tubes could not pass through pistils with aborted transmitting tissue, and expression of an NpGUT1 antisense gene in germinating pollen inhibited pollen tube elongation, accompanied by the absence of pectin RG-II and boron in the pollen tube tip. These results indicate that expression of NpGUT1 is required for the development and functions of male and female tissues.
Iwai
Hiroaki
H
Graduate School of Life and Environmental Sciences, Division of Integrative Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Hokura
Akiko
A
Oishi
Masahiro
M
Chida
Hiroshi
H
Ishii
Tadashi
T
Sakai
Shingo
S
Satoh
Shinobu
S
D001881:Borates / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000378:metabolism*; D016373:DNA, Antisense / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D014453:Glucuronosyltransferase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010368:Pectins / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011058:Pollen / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
D001881:Borates; D003432:Cross-Linking Reagents; D016373:DNA, Antisense; D010940:Plant Proteins; C042492:rhamnogalacturonan II; D010368:Pectins; C111364:galactosylgalactoylxylosylprotein 3-beta-glucuronosyltransferase; D014453:Glucuronosyltransferase