Improved sensitivity and physical properties of sol-gel protein chips using large-scale material screening and selection.
Protein chips are a powerful emerging technology with extensive biomedical applications. However, the development of optimal, economical surface materials capable of maintaining the activity of embedded proteins is a challenge. Here, we introduce a new optimized, low-cost, sol-gel biomaterial for use in protein chips with femtogram-level sensitivity. A novel protein chip material with significantly improved physical properties and sensitivity was produced using unique screening and selection methods. Using this platform, the sensitive, specific detection of the interactions between an HIV antigen and its antibody and between a cyclin-kinase protein pair was observed. This study is the first to demonstrate the detection of protein-protein interactions on sol-gel microarrays and describes an important improvement in the physical properties of sol-gel-derived protein chip materials for biomedical research.
Kim
Soyoun
S
Chemistry Department, Dongguk University, Seoul, Korea. skim@dongguk.edu
Kim
Youngdeuk
Y
Kim
Philseok
P
Ha
Jeongmin
J
Kim
Kyunyoung
K
Sohn
Mijin
M
Yoo
Jin-San
JS
Lee
Jungeun
J
Kwon
Jung-Ah
JA
Lee
Kap No
KN
D000906:Antibodies / Q000737:chemistry; D005782:Gels; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000906:Antibodies; D005782:Gels; D011506:Proteins