title: [Studies on HPLC fingerprint of Radix Aconiti Kusnezoffii].
abstract: OBJECTIVE
  To establish the HPLC fingerprint of Radix Aconiti Kusnezoffii.
  
  
  METHODS
  The chromatographic separation was performed on a Kromasil C18 (4.6 mm x 250 mm, 5 microm) eluted with a mobile phase of acetonitrile-ammonium acetate buffer (2. 5 per thousand acetic acid-ammonia pH 10.5) (60:40). The UV detection wavelength was set at 240 nm and the flow rate was set at 1.0 mL x min(-1).
  
  
  RESULTS
  The RSD of precision and repeatability was less than 2%. Under the selected chromatographic conditions, good HPLC fingerprints of Radix Aconiti Kusnezoffii were obtained.
  
  
  CONCLUSIONS
  The method was simple, accurate and repeatable. It can be used for the quality control of Radix Aconiti Kusnezoffii.
authors.affiliation: Wenshan Prefecture Sanqi Science and Technology Research Institute, China.
authors.forename: Ying-yong
authors.initials: YY
authors.lastname: Zhao
authors.forename: Xiu-ming
authors.initials: XM
authors.lastname: Cui
authors.forename: Yun
authors.initials: Y
authors.lastname: Dai
authors.forename: Hua
authors.initials: H
authors.lastname: Miao
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives* / Q000032:analysis; D031841:Aconitum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: C019470:mesaconitine; D000157:Aconitine