title: Proteome analysis of recombinant Escherichia coli producing human glucagon-like peptide-1.
abstract: The proteomic response of recombinant Escherichia coli producing human glucagon-like peptide-1 was analyzed by two-dimensional gel electrophoresis. Protein spots in two-dimensional gel could be identified by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and their expression profiles were compared with those of nonproducing cells. Thirty-five intracellular proteins exhibited differential expression levels between the production and control strains. These changes reflected physiological responses to heterologous peptide production in recombinant E. coli. Specifically, physiological changes included the down-regulation of proteins involved in the central carbon metabolism, biosynthesis of cellular building blocks and peptides, and up-regulation of cell protection proteins and some sugar transport proteins. This comprehensive analysis would provide useful information for understanding physiological alterations to heterologous peptide production and for designing efficient metabolic engineering strategies for the production of recombinant peptides in E. coli.
authors.affiliation: Department of Agricultural Biotechnology and The BioMAX Institute, Seoul National University, Seoul 151-921, South Korea.
authors.forename: Dae-Hee
authors.initials: DH
authors.lastname: Lee
authors.forename: Sung-Gun
authors.initials: SG
authors.lastname: Kim
authors.forename: Yong-Cheol
authors.initials: YC
authors.lastname: Park
authors.forename: Soo-Wan
authors.initials: SW
authors.lastname: Nam
authors.forename: Kelvin H
authors.initials: KH
authors.lastname: Lee
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Seo
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D052216:Glucagon-Like Peptide 1 / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D006801:Humans; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D011994:Recombinant Proteins / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D020543:Proteome; D011994:Recombinant Proteins; D052216:Glucagon-Like Peptide 1