title: Response of human endothelial cells to oxidative stress on Ti6Al4V alloy.
abstract: Titanium and its alloys are amongst the most frequently used materials in bone and dental implantology. The good biocompatibility of titanium(-alloys) is attributed to the formation of a titanium oxide layer on the implant surface. However, implant failures do occur and this appears to be due to titanium corrosion. Thus, cells participating in the wound healing processes around an implanted material, among them endothelial cells, might be subjected to reactive oxygen species (ROS) formed by electrochemical processes during titanium corrosion. Therefore, we studied the response of endothelial cells grown on Ti6Al4V alloy to H(2)O(2) and compared this with the response of endothelial cells grown on cell culture polystyrene (PS). We could show that although the cell number was the same on both surfaces, metabolic activity of endothelial cells grown on Ti6Al4V alloy was reduced compared to the cells on PS and further decreased following prototypic oxidative stress (H(2)O(2)-treatment). The analysis of H(2)O(2)-induced oxidative stress showed a higher ROS formation in endothelial cells on Ti6Al4V than on PS. This correlated with the depletion of reduced glutathione (GSH) in endothelial cells grown on Ti6Al4V surfaces and indicated permanent oxidative stress. Thus, endothelial cells in direct contact with Ti6Al4V showed signs of oxidative stress and higher impairment of cell vitality after an additional oxidative stress. However, the exact nature of the agent of oxidative stress generated from Ti6Al4V remains unclear and requires further investigation.
authors.affiliation: Institute of Pathology, Johannes Gutenberg-University Mainz, Langenbeckstr. 1, 55101 Mainz, Germany.
authors.forename: Roman
authors.initials: R
authors.lastname: Tsaryk
authors.forename: Marie
authors.initials: M
authors.lastname: Kalbacova
authors.forename: Ute
authors.initials: U
authors.lastname: Hempel
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Unger
authors.forename: Peter
authors.initials: P
authors.lastname: Dieter
authors.forename: C James
authors.initials: CJ
authors.lastname: Kirkpatrick
authors.forename: Kirsten
authors.initials: K
authors.lastname: Peters
mesh_terms: D000497:Alloys; D000975:Antioxidants / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007249:Inflammation; D008422:Materials Testing; D018384:Oxidative Stress; D019736:Prostheses and Implants; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D014025:Titanium / Q000494:pharmacology*
chemical_list: D000497:Alloys; D000975:Antioxidants; D020099:Coated Materials, Biocompatible; D017382:Reactive Oxygen Species; C031462:titanium alloy (TiAl6V4); D006861:Hydrogen Peroxide; D014025:Titanium; D013482:Superoxide Dismutase; D005978:Glutathione