The effect of Tween80 on signal intensity of intact proteins by MALDI time-of-flight mass spectrometry.
Buffers and detergents are notorious for suppression of analyte signal in electrospray and MALDI mass spectrometry and, invariably, analysts will take steps to remove these contaminants before MS analysis. However, we have found serendipitously that protein signal with MALDI MS is improved by about an order of magnitude on the addition of small amounts of Tween80. Four charged states of BSA could easily be seen at less than 125 fmol/spot and with mixture of three proteins (BSA, trypsinogen, and protein A) the molecular ions could be detected on as little as 12.5 fmol of spotted material (per protein) using an automated laser firing sequence.
Brinkworth
Craig S
CS
Human Protection and Performance Division, Defence, Science, and Technology Organisation, Victoria, Australia. craig.brinkworth@dsto.defence.gov.au
Bourne
David J
DJ
D000818:Animals; D002417:Cattle; D011136:Polysorbates / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D011136:Polysorbates; D012710:Serum Albumin, Bovine