title: Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: detection of authentic Marburgvirus.
abstract: There have recently been large outbreaks of Marburg hemorrhagic fever (MHF) caused by Marburgvirus (MARV) in the Democratic Republic of Congo and Angola. The development of reliable diagnostic systems for MHF is urgently needed. An antigen-capture enzyme-linked immunosorbent assay (Ag-capture ELISA) using either of the two monoclonal antibodies (2A7 and 2H6) produced by immunizing mice with recombinant nucleoprotein of MARV was described (Journal of Medical Virology, 76, 111-118, 2005). In the present study, it was revealed that the Ag-capture ELISA specifically detected authentic MARV antigen and that the sensitivity of the Ag-capture ELISA was at a level similar to that of reverse-transcription polymerase chain reaction. These results suggest that the Ag-capture ELISA using the monoclonal antibodies, 2A7 and 2H6, is applicable to the diagnosis of MHF.
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208-0011, Japan. msaijo@nih.go.jp
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Georges-Courbot
authors.forename: Shuetsu
authors.initials: S
authors.lastname: Fukushi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.forename: Marianneau
authors.initials: M
authors.lastname: Philippe
authors.forename: Alain-Jean
authors.initials: AJ
authors.lastname: Georges
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008379:Marburg Virus Disease / Q000821:virology; D029024:Marburgvirus / Q000302:isolation & purification*; D051379:Mice; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012261:Ribonucleoproteins / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D014764:Viral Proteins; C073094:nucleoprotein, Marburg virus