Effects of inoculum size and incubation time on broth microdilution susceptibility testing of lactic acid bacteria.
Inoculum size and incubation time were varied during broth microdilution testing of the susceptibilities of 35 strains of lactic acid bacteria to six antibiotics. An increase in either parameter resulted in elevated MICs for all species. An inoculum of 3 x 10(5) CFU/ml is recommended to assess the antibiotic susceptibilities of these bacteria by using broth microdilution.
Egervärn
Maria
M
Microbiology Division, National Food Administration, Box 622, SE-751 26 Uppsala, Sweden. mia.egervarn@slv.se
Lindmark
Hans
H
Roos
Stefan
S
Huys
Geert
G
Lindgren
Sven
S
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D007777:Lactobacillaceae / Q000145:classification / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests / Q000379:methods*; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D003470:Culture Media