Qualification and performance characteristics of a quantitative enzyme-linked immunosorbent assay for human lgG antibodies to anthrax lethal factor antigen.
The contribution of Bacillus anthracis lethal factor (LF)-specific immune responses to protection against anthrax disease in humans remains incompletely defined due, in part, to a paucity of qualified reagents and a lack of standardized serological assays. Toward this end, we have identified and characterized suitable positive quality control and standard reference sera and developed, optimized, and qualified an enzyme-linked immunosorbent assay (ELISA) to measure LF-binding IgG. Herein we describe the performance characteristics of this ELISA and propose criteria for its use in the detection and quantification of anti-LF IgG in human serum.
Selinsky
Cheryl L
CL
The Biodesign Institute at Arizona State University, Tempe, AZ, USA.
Whitlow
Vanessa D
VD
Smith
Larry R
LR
Kaslow
David C
DC
Horton
Holly M
HM
D000881:Anthrax / Q000517:prevention & control; D000942:Antigens, Bacterial / Q000276:immunology*; D000992:Antitoxins / Q000032:analysis* / Q000627:therapeutic use; D001427:Bacterial Toxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D012015:Reference Standards; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial; D000992:Antitoxins; D001427:Bacterial Toxins; D007074:Immunoglobulin G; C030325:anthrax toxin