[Effects of ligustrazine on hippocampal dentate gyrus cell proliferation after focal cerebral ischemia in adult rats].
OBJECTIVE
To explore the effects of ligustrazine on cell proliferation in hippocampal dentate gyrus subgranular zone (SGZ) after focal cerebral ischemia in adult rats.


METHODS
Middle cerebral artery occlusion (MCAO) model was established in adult rats by placement of an intraluminal filament at the origin of the MCA. Ligustrazine was administered intraperitoneally at a daily dose of 80 mg/kg starting at 2 h after MCAO, and BrdU (50 mg/kg daily) was also injected intraperitoneally starting at 4 h after MCAO. BrdU-positive cells in the SGZ were counted 7, 14 and 24 days after MCAO, respectively.


RESULTS
Compared with sham operation group, ischemic ipsilateral BrdU-positive cells in the ischemic model group increased 7 days after MCAO, reaching the peak on day 14, and decreased on day 21 (P<0.01). The number of ischemic ipsilateral BrdU-positive cells in ligustrazine group was significantly greater than that in the ischemic model group on days 7, 14 and 21 (P<0.01), and maintained the high level on day 21.


CONCLUSIONS
Ligustrazine possesses long lasting effect of promoting cell proliferation in the SGZ after focal cerebral ischemia in adult rats.
Qiu
Fen
F
Research Center for Neuroscience, Key Laboratory of Environment and Genes Related Diseases of Ministry of Education, Medical School of Xi'an Jiaotong University, Xi'an 710061, China. qiufen6688@mail.xjtu.edu.cn
Liu
Yong
Y
Zhang
Peng-bo
PB
Tian
Ying-fang
YF
Qi
Cun-fang
CF
Zhao
Jian-jun
JJ
Yang
Jie
J
Xiao
Xin-li
XL
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D018891:Dentate Gyrus / Q000187:drug effects* / Q000473:pathology; D020244:Infarction, Middle Cerebral Artery / Q000503:physiopathology*; D007274:Injections, Intraperitoneal; D008297:Male; D011719:Pyrazines / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D011719:Pyrazines; C017953:tetramethylpyrazine