title: Genome wide cDNA-AFLP analysis of genes rapidly induced by combined sucrose and ABA treatment in rice cultured cells.
abstract: We identified 27 genes induced by combined sucrose and ABA treatment from rice cultured cells with cDNA-AFLP. Thirteen of these up-regulated genes were induced 30 min after the co-treatment. This suite of genes includes starch biosynthesis related genes. Type A genes were expressed only in the presence of both sucrose and ABA. Type B genes were expressed in the presence of sucrose or ABA and the expression was dramatically enhanced by the co-treatment of sucrose and ABA. These results indicate that multiple steps of starch biosynthesis and other processes may be regulated by at least two different pathways.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan. takihiro@mua.biglobe.ne.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Akihiro
authors.forename: Taishi
authors.initials: T
authors.lastname: Umezawa
authors.forename: Chie
authors.initials: C
authors.lastname: Ueki
authors.forename: Ben Mohamed
authors.initials: BM
authors.lastname: Lobna
authors.forename: Kouichi
authors.initials: K
authors.lastname: Mizuno
authors.forename: Masaru
authors.initials: M
authors.lastname: Ohta
authors.forename: Tatsuhito
authors.initials: T
authors.lastname: Fujimura
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D001483:Base Sequence; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000187:drug effects*; D018745:Genome, Plant; D021141:Nucleic Acid Amplification Techniques; D012275:Oryza / Q000187:drug effects* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013395:Sucrose / Q000494:pharmacology*
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D013395:Sucrose; D000040:Abscisic Acid