title: Analysis of immune cell infiltrates during squamous carcinoma development.
abstract: Infiltration of leukocytes into tissue is a common feature of many physiological and pathological conditions. Histopathologically, the diversity of leukocytes that infiltrate a tissue associated with a pathophysiologic response cannot be appreciated and/or examined unless highly selective immunologic detection methods are utilized. Specific populations of infiltrating leukocytes into squamous tissues harboring pre-malignant and/or malignant keratinocytes have recently been demonstrated to play a functionally significant role in the pathogenesis of squamous carcinomas. To evaluate immune cell types and quantify changes in their relative presence and localization during multi-stage neoplastic progression, we performed flow cytometry and histochemical detection using lineage-selective markers. Herein, we provide detailed methodology facilitating these analyses.
authors.affiliation: Cancer Research Institute, University of California, San Francisco, San Francisco, California 94143, USA.
authors.forename: Simon R
authors.initials: SR
authors.lastname: Junankar
authors.forename: Alexandra
authors.initials: A
authors.lastname: Eichten
authors.forename: Annegret
authors.initials: A
authors.lastname: Kramer
authors.forename: Karin E
authors.initials: KE
authors.lastname: de Visser
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Coussens
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000276:immunology / Q000473:pathology*; D002465:Cell Movement; D005434:Flow Cytometry; D007113:Immunity, Innate; D007150:Immunohistochemistry; D007962:Leukocytes / Q000502:physiology*; D008264:Macrophages / Q000502:physiology*; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000032:analysis; D012878:Skin Neoplasms / Q000209:etiology / Q000276:immunology / Q000473:pathology*
chemical_list: D016186:Receptor, Macrophage Colony-Stimulating Factor