title: The expression patterns of RGS transcripts in platelets.
abstract: Regulators of G protein signalling (RGS) are involved in the negative regulation of cell activation processes and are involved in the pathophysiology of cardiovascular diseases. To get some further evidence for a role of RGS proteins in platelets, we determined the expression profile of RGS-specific mRNA in rat platelets using reverse transcription-polymerase chain reaction (RT-PCR) with a poly dT18 primer and transcript-specific primers. We found that RGS2, RGS3, RGS5, RGS6, RGS10, RGS14, RGS16 and RGS18, Leukemia-associated Rho-GEF factor (LARG), and Galpha interacting protein (GAIP) were differentially expressed in platelets. The highest expression rate was found for RGS18 (about 1.3 fold when compared to GAPDH), followed by LARG, RGS6, RGS10 and RGS16 (0.7 to 0.95), whereas expression rates for RGS2, RGS3, RGS5, RGS14, and GAIP were in a range of 0.1 to 0.3. Our results suggest that G-protein-coupled receptor-mediated signalling in platelet may be regulated mainly by RGS 18, 16, 10, 6, and LARG.
authors.affiliation: Laboratory of Veterinary Physiology & Signalling, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Sung Dae
authors.initials: SD
authors.lastname: Kim
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Sung
authors.forename: Sun Kyu
authors.initials: SK
authors.lastname: Park
authors.forename: Tae Wan
authors.initials: TW
authors.lastname: Kim
authors.forename: Seung Chun
authors.initials: SC
authors.lastname: Park
authors.forename: Sang Keun
authors.initials: SK
authors.lastname: Kim
authors.forename: Jae Youl
authors.initials: JY
authors.lastname: Cho
authors.forename: Man Hee
authors.initials: MH
authors.lastname: Rhee
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D020662:Guanine Nucleotide Exchange Factors / Q000502:physiology; D020710:RGS Proteins / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D064067:Rho Guanine Nucleotide Exchange Factors; D015398:Signal Transduction
chemical_list: C577224:Arhgef12 protein, rat; D020662:Guanine Nucleotide Exchange Factors; D020710:RGS Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D064067:Rho Guanine Nucleotide Exchange Factors