[Determination of fraxinellone in root bark of Dictamnus dasycarpus by RP-HPLC].
OBJECTIVE
To develop a RP-HPLC method for determination of fraxinellone in Dictamnus dasycarpus.


METHODS
RP-HPLC conditions were as follows: AT-LICHROM C18(4.6 mm x 250 mm) column, MeOH-H2O (70:30) as a mobile phase, detection wavelength 240 nm, column temperature 25 degrees C, Flow velocity 0.5 mL x min(-1).


RESULTS
Linear relationship is very good (r = 0.9999) in 0.018-0.18 mg x mL(-1). The average recovery is 98.9%, RSD is 1.2% (n = 4).


CONCLUSIONS
The method is simple and repeatable and can be used for quantitative analysis of fraxinellone in root bark of D. dasycarpus.
Yuan
Chun-lan
CL
Department of Chemistry & Chemical Engineering, Baoji University of Arts and Sciences, Baoji 721007, China. ycl2005999@126.com
Yang
De-suo
DS
D001572:Benzofurans / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D036905:Dictamnus / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D001572:Benzofurans; C075391:fraxinellone