title: [Determination of akebia saponin D in root of Dipacus asperoides by HPLC].
abstract: OBJECTIVE
  To develop a quantitative method for the determination of akebia saponin D in root of Dipacus asperoides and provide scientific basis for quality control of D. asperoides.
  
  
  METHODS
  An IPLC analytical method was established using Kromasil ODS column with acetonitrile-water (30:70) as mobile phase and the detection wavelength was 212 nm.
  
  
  RESULTS
  The akebia saponin D in root extract were well separated by this method. The linear range is between 0.582 5-9.32 microg, r = 0.9999. The average recovery and RSD of repeatability are 100.3%, 2.3%, respectively.
  
  
  CONCLUSIONS
  This method is simple, accurate and reliable by evaluating the method validation data and can be used for the quality control of D. asperoides and its preparations.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing.
authors.forename: Hong-gen
authors.initials: HG
authors.lastname: Tan
authors.forename: Sheng
authors.initials: S
authors.lastname: Lin
authors.forename: Qi-wei
authors.initials: QW
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Ji
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D049917:Dipsacaceae / Q000737:chemistry*; D017753:Ecosystem; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012503:Saponins / Q000032:analysis*
chemical_list: D012503:Saponins