In-vitro functional phenotypes of plasma cell lines from patients with multiple myeloma.
Seven plasma cell lines from patients with smoldering (group A) and overt myeloma (group B) were investigated for both phenotypic markers and in-vitro properties, including sensitivity to apoptosis, cytotoxicity, cell adhesion, chemotaxis and bone interaction. Cell lines from group A underwent apoptosis whereas those from group B were apparently resistant, promoted cytotoxicity in target cells and enhanced both adhesion and migratory functions upon appropriate activators. In addition, MCC-2, a group B cell line from a patient with severe osteolytic disease of the skeleton produced erosive lacunae on bone substrates, whereas this effect was almost absent with cell lines from group A. Concurrent deregulation of relative markers, in combination with peculiar properties including resistance to apoptosis and high cytotoxic potential, as well as adhesion, chemotaxis and bone pathophysiology interactions, may thus identify myeloma cells with aggressive phenotype driving these biological activities in vitro and perhaps in vivo.
Silvestris
Franco
F
Department of Internal Medicine and Oncology (DIMO), University of Bari, Piazza Giulio Cesare 11, 70124 Bari, Italy. f.silvestris@dmo.uniba.it
Cafforio
Paola
P
Calvani
Nicola
N
De Matteo
Monica
M
Lombardi
Lucia
L
Dammacco
Franco
F
D017209:Apoptosis; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D002633:Chemotaxis; D016207:Cytokines; D006801:Humans; D066298:In Vitro Techniques; D009101:Multiple Myeloma / Q000145:classification / Q000473:pathology*; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology*; D010641:Phenotype; D010950:Plasma Cells / Q000473:pathology*; D014407:Tumor Cells, Cultured
D016207:Cytokines; C025940:bone resorption factor