Adenosine kinase from rat liver: new biochemical properties.
Adenosine kinase is a well-known enzyme which catalyzes the phosphorylation of adenosine to AMP: Its metabolic and kinetic properties are well studied. Here, we report new properties of rat liver enzyme, demonstrating a new reaction: ADP can be a phosphate donor instead ATP, according to the reaction: adenosine + ADP --> 2AMP) demonstrating the efficiency of AdK to phosphorylate adenosine, also starting from ADP. Cells could exploited this property in situations in which ATP levels are strongly decreased and ADP decreases slowly.
Leoncini
R
R
Department of Internal Medicine, Endocrine-Metabolic Sciences and Biochemistry University of Siena, Siena, Italy.
Vannoni
D
D
Santoro
A
A
Giglioni
S
S
Carli
R
R
Marinello
E
E
D000244:Adenosine Diphosphate / Q000737:chemistry / Q000494:pharmacology; D000248:Adenosine Kinase / Q000737:chemistry / Q000502:physiology*; D000249:Adenosine Monophosphate / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002384:Catalysis; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D015636:Magnesium Chloride / Q000494:pharmacology; D009711:Nucleotides / Q000737:chemistry*; D010766:Phosphorylation; D011687:Purines / Q000737:chemistry; D051381:Rats
D009711:Nucleotides; D011687:Purines; D015636:Magnesium Chloride; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000248:Adenosine Kinase