Peroxinectin gene transcription of the giant freshwater prawn Macrobrachium rosenbergii under intrinsic, immunostimulant, and chemotherapeutant influences.
Peroxinectin (PE) gene expressions were determined using real-time PCR in the giant freshwater prawn Macrobrachium rosenbergii based on moulting; prawns were fed diets containing different concentrations of sodium alginate, and were exposed to different concentrations of copper sulphate, benzalkonium chloride (BKC), and trichlorfon. Results showed that PE mRNA expression of prawns was the highest in stage A, significantly decreased in stage B, and reached the lowest level in stages D0/D1. The PE transcript was significantly higher in prawns fed the 1.0 gkg(-1) sodium alginate-containing diet than those fed the 2.0 gkg(-1) sodium alginate-containing diet and those fed the control diet. PE transcripts significantly decreased in prawns exposed to 0.1-0.4 mgL(-1) copper sulphate after 96 h, 0.3-1.0 mgL(-1) BKC after 96 h, and 0.2-0.4 mgL(-1) trichlorfon after 48 h. It was concluded that the status of PE gene expression was seriously affected by the moult cycle, immunostimulant, and chemotherapeutants.
Liu
Chun-Hung
CH
Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, ROC.
Yeh
Shinn-Pyng
SP
Hsu
Pei-Yi
PY
Cheng
Winton
W
D000464:Alginates / Q000008:administration & dosage / Q000633:toxicity; D000818:Animals; D001548:Benzalkonium Compounds / Q000008:administration & dosage / Q000633:toxicity; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics; D019327:Copper Sulfate / Q000008:administration & dosage / Q000633:toxicity; D018076:DNA, Complementary / Q000032:analysis / Q000096:biosynthesis; D015870:Gene Expression / Q000187:drug effects* / Q000502:physiology; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000633:toxicity; D006434:Hemocytes / Q000187:drug effects; D006490:Hemostatics / Q000008:administration & dosage / Q000494:pharmacology; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000633:toxicity; D033541:Palaemonidae / Q000187:drug effects* / Q000276:immunology; D013997:Time Factors; D014236:Trichlorfon / Q000008:administration & dosage / Q000494:pharmacology; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*
D000464:Alginates; D001548:Benzalkonium Compounds; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D006490:Hemostatics; D006603:Hexuronic Acids; D014874:Water Pollutants, Chemical; C511086:peroxinectin protein, Macrobrachium rosenbergii; D020723:Glucuronic Acid; D014236:Trichlorfon; D019327:Copper Sulfate