Application of (31P) NMR in analyzing the degradation efficiency of organic phosphorus degrading-bacteria.
HPLC and HPLC-MS are the fastest and most accurate techniques for analysis of organic phosphorus pesticide (OPP) at the present time. Using these techniques, 14 strains of methamidopho (MAP) degrading-bacteria from the area contaminated with MAP have been identified. The results from HPLC and HPLC-MS analyses showed that the highest degradation rate was 73% after 7 days. In order to determine what metabolites will be formed after degradation, a key issue that has been neglected for a long time, we used ((31)P) NMR to track the degradation process. The results showed that different strains produced different metabolites. Ten strains were divided into three groups (groups A, B and C) by their metabolic profiling. Strains in group A degraded MAP into phosphor acid by breaking down all P-N, P-O and P-S bonds in 7 days. Strains in groups B and C had only broken down partially P-N and P-S bonds at the same time. Therefore, the bacterial strains in group A had a greater application potential than the other two groups. In addition, most metal phosphates are unsolvable in water. The analysis of X-ray showed, that the phosphate radicals generated by bacterial degradation induce crystallogenesis of heavy metal salts in water phase and also cause the chemical sedimentation of their crystals. Furthermore, these crystals are hydrogen phosphates. The results suggested that the MAP-degrading bacteria could be used for cleaning up not only the organic phosphorous pesticide contamination but also the phosphorous and heavy metal contamination in water environment simultaneously.
Lu
Yang
Y
Department of Chemistry and The Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, People's Republic of China.
Sun
Xin
X
Ji
Si-Yao
SY
Wang
Jian-Feng
JF
Huang
Yao-Jian
YJ
Zhao
Yu-Fen
YF
Xu
Peng-Xiang
PX
D001419:Bacteria; D001673:Biodegradation, Environmental; D002681:China; D017753:Ecosystem; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009943:Organophosphorus Compounds / Q000009:adverse effects / Q000032:analysis* / Q000145:classification; D010573:Pesticide Residues; D045483:Rivers
D009943:Organophosphorus Compounds; D010573:Pesticide Residues