title: Structure determination of VEGF-E by sulfur SAD.
abstract: The crystal structure of VEGF-E was solved by the sulfur single-wavelength anomalous dispersion method (S-SAD) using highly redundant low-resolution data collected at a wavelength of lambda approximately 1.7 A with an estimated anomalous signal of 1.5%. 11 sulfur sites, nine out of 16 disulfide bonds and two out of 12 methionines could be located in the asymmetric unit using data truncated at a resolution of 4.1 A; however, none of the common diffraction data-quality indicators for SAD allowed clear discrimination between successful and unsuccessful resolution cutoffs. The high solvent content of 75% allowed efficient density modification to be performed and an unbiased electron-density map of good quality to be generated. This study demonstrates the strength of S-SAD for phasing using accurate highly redundant data at low resolution.
authors.affiliation: Swiss Light Source, Paul Scherrer Institut, Villigen-PSI, Switzerland.
authors.forename: Armin
authors.initials: A
authors.lastname: Wagner
authors.forename: Michel
authors.initials: M
authors.lastname: Pieren
authors.forename: Clemens
authors.initials: C
authors.lastname: Schulze-Briese
authors.forename: Kurt
authors.initials: K
authors.lastname: Ballmer-Hofer
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Prota
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods; D004220:Disulfides / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D013455:Sulfur / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry*
chemical_list: D004220:Disulfides; D042461:Vascular Endothelial Growth Factor A; D013455:Sulfur