title: Short-term effects of IGF-I and estradiol on LH secretion from female rat gonadotrophs.
abstract: OBJECTIVE
  Growth factors and ovarian steroids modulate LH-secretion from pituitary gonadotrophs. Our previous studies demonstrated that long-term IGF-I treatment enhanced LH-secretion from female rat pituitary cells and estradiol facilitated this effect. The effects of estradiol on LH secretion are time-dependent. Short-term treatment inhibited, long-term treatment enhanced GnRH-induced LH-secretion in serum-containing medium. Here we tested the short-term actions of IGF-I and its interaction with estradiol and whether IGF-I is a prerequisite for the negative effect of short-term estradiol treatment in female rat pituitary cells.
  
  
  METHODS
  Pituitary cells were incubated with a series of increasing concentrations of estradiol (1 pM, 10 pM, 50 pM, 100 pM, 500 pM, 1 nM, 10 nM and 100 nM) for 4 h, IGF-I (10 pM, 100 pM, 1 nM and 10 nM) for 4 h and 14 h and their combinations for 4h in serum-free medium, and then stimulated with 1 nM GnRH during the last 3h of incubation. To clarify the role of IGF-I, cells were incubated simultaneously with estradiol, IGF-I and antibody against IGF-I. LH was measured by radioimmunoassay.
  
  
  RESULTS
  Short-term IGF-I treatment did not modify basal or GnRH-induced LH-secretion. Short-term treatment with estradiol did not affect basal or GnRH-induced LH-secretion in serum-free medium. The addition of 100 pM IGF-I to serum-free medium established the negative effect of estradiol short-term treatment on GnRH-induced LH-secretion. The addition of IGF-I antibody fully abolished the negative effect of estradiol.
  
  
  CONCLUSIONS
  In conclusion, effects of IGF-I on LH-secretion in female rat pituitary cells require long-term treatment. The negative effect of estradiol short-term treatment on GnRH-induced LH-secretion is dependent on serum-containing medium or the addition of 100 pM IGF-I to serum-free medium.
authors.affiliation: Department of Obstetrics and Gynaecology, Medical University of Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany. jmweiss1@hotmail.com
authors.forename: J-M
authors.initials: JM
authors.lastname: Weiss
authors.forename: Y-X
authors.initials: YX
authors.lastname: Xia
authors.forename: S
authors.initials: S
authors.lastname: Polack
authors.forename: K
authors.initials: K
authors.lastname: Diedrich
authors.forename: O
authors.initials: O
authors.lastname: Ortmann
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D052681:Gonadotrophs / Q000187:drug effects* / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D007986:Luteinizing Hormone