title: Local mutagenic impact of insertions of LTR retrotransposons on the mouse genome.
abstract: Solitary LTR loci are the predominant form of LTR retrotransposons in most eukaryotic genomes. They originate from recombination between the two LTRs of an ancestral retrovirus and are therefore incapable of transposition. Despite this inactivity, they appear to have a substantial impact on the host genome. Here we use the murine RMER10 LTR family as an example to describe how such elements can reshape regions of the genome through multiple mutations on an evolutionary time scale. Specifically, we use phylogenetic analysis of multiple copies of RMER10 in rodent species, as well as comparisons of orthologous pairs in mouse and rat, to argue that insertions of members of this family have locally induced the emergence of tandem repeat loci as well as many indels. Analysis of structural aspects of these sequences (secondary structures and transcription factors signals) may explain why RMER10 can become endogenous "mutagenic" factors through induction of replication fork blockages and/or error-prone repair of aberrant DNA structures. This hypothesis is also consistent with features of other interspersed repeated elements.
authors.affiliation: Laboratoire Génome, Populations, Interactions, Adaptation, UMR5171 CNRS-IFREMER, Université Montpellier II, CC-G3 Montpellier Place E. Bataillon 34095, France. desmarais@univ-montp2.fr
authors.forename: Erick
authors.initials: E
authors.lastname: Desmarais
authors.forename: Khalid
authors.initials: K
authors.lastname: Belkhir
authors.forename: John Carlos
authors.initials: JC
authors.lastname: Garza
authors.forename: François
authors.initials: F
authors.lastname: Bonhomme
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D016678:Genome / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D051381:Rats; D018626:Retroelements / Q000235:genetics*; D016415:Sequence Alignment; D020079:Terminal Repeat Sequences / Q000235:genetics*
chemical_list: D018626:Retroelements; D004247:DNA