title: Soy protein containing isoflavones favorably influences macrophage lipoprotein metabolism but not the development of atherosclerosis in CETP transgenic mice.
abstract: The possibility that soy protein containing isoflavones influences the development of experimental atherosclerosis has been investigated in ovariectomized mice heterozygous for the human CETP transgene and for the LDL-receptor null allele (LDLr(+/-) CETP(+/-)). After ovariectomy at 8 wk of age they were fed a fat/cholesterol-rich diet for 19 wk and divided into three experimental groups: dietary unmodified soy protein containing isoflavones (mg/g of diet), either at low-dose (Iso Low, 0.272, n = 25), or at high-dose (Iso High, 0.535, n = 28); and the atherogenic diet containing an isoflavone-depleted alcohol-washed soy protein as a control group (n = 28). Aortic root lipid-stained lesion area (mean microm2 x 10(3) +/- SD) did not differ among Iso Low (12.3 +/- 9.9), Iso High (7.4 +/- 6.4), and controls (10.7 +/- 12.8). Autoantibody titers against plasma oxidized LDL did not differ among the experimental groups. Using the control mice as the reference value (100%), in vitro mouse peritoneal macrophage uptake of labeled acetylated LDL-cholesterol was lower in the Iso High (68%) than in the Iso Low (85%) group. The in vitro percent removal by exogenous HDL of labeled unesterified cholesterol from macrophages previously enriched with human [4- 14C]-cholesteryl oleate acetylated LDL was enhanced in the Iso High group (50%). In spite of these in vitro potentially antiatherogenic actions, soy protein containing isoflavones did not modify the average size of lipid-stained area in the aortic root.
authors.affiliation: Lipids Lab, University of São Paulo Medical School, São Paulo, Brazil.
authors.forename: Leiko
authors.initials: L
authors.lastname: Asakura
authors.forename: Patrícia M
authors.initials: PM
authors.lastname: Cazita
authors.forename: Lila M
authors.initials: LM
authors.lastname: Harada
authors.forename: Valéria S
authors.initials: VS
authors.lastname: Nunes
authors.forename: Jairo A
authors.initials: JA
authors.lastname: Berti
authors.forename: Alessandro G
authors.initials: AG
authors.lastname: Salerno
authors.forename: Daniel F J
authors.initials: DF
authors.lastname: Ketelhuth
authors.forename: Magnus
authors.initials: M
authors.lastname: Gidlund
authors.forename: Helena C F
authors.initials: HC
authors.lastname: Oliveira
authors.forename: Eder C R
authors.initials: EC
authors.lastname: Quintão
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000178:diet therapy / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D053480:Cholesterol Ester Transfer Proteins; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006579:Heterozygote; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood / Q000378:metabolism*; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010052:Ovariectomy; D011973:Receptors, LDL / Q000235:genetics; D030262:Soybean Proteins / Q000737:chemistry / Q000494:pharmacology*; D019076:Transgenes
chemical_list: C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D006023:Glycoproteins; D007529:Isoflavones; D008055:Lipids; D008074:Lipoproteins; D011973:Receptors, LDL; D030262:Soybean Proteins