Medicinal plant extracts as anti-Escherichia coli O157:H7 agents and their effects on bacterial cell aggregation.
Ethanolic extracts of eight Thai medicinal plants (representing five families) that are used as traditional remedies for treating diarrhea were examined with a salt aggregation test for their ability to modulate cell surface hydrophobicity of enterohemorrhagic Escherichia coli strains, including E. coli O157:H7. Four of these medicinal plants, Acacia catechu, Peltophorum pterocarpum, Punica granatum, and Quercus infectoria, have high bacteriostatic and bactericidal activities. The ethanolic extract of Q. infectoria was the most effective against all strains of E. coli, with MICs of 0.12 to 0.98 mg/ml and MBCs of 0.98 to 3.91 mg/ml. The ethanolic extract of P. granatum had MICs of 0.49 to 1.95 mg/ml and MBCs of 1.95 to 3.91 mg/ml. Ethanolic extracts of Q. infectoria, P. pterocarpum, and P. granatum were among the most effective extracts against the two strains of E. coli O157:H7. The other four plants, Andrographis paniculata, Pluchia indica, Tamarindus indica, and Walsura robusta, did not have high bacteriostatic and bactericidal activities but were able to affect hydrophobicity characteristics on their outermost surface. All plants except Q. infectoria had some ability to increase cell surface hydrophobicity. There appears to be no correlation between antibacterial activity and cell aggregative properties.
Voravuthikunchai
Supayang Piyawan
SP
Department of Microbiology, Faculty of Science, Prince of Songkla University, Hatyai, Songkla 90112, Thailand. supayang.v@psu.ac.th
Limsuwan
Surasak
S
D000045:Acacia / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004927:Escherichia coli Infections / Q000188:drug therapy*; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000254:growth & development; D007887:Fabaceae / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D029561:Lythraceae / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D029963:Quercus / Q000737:chemistry
D000900:Anti-Bacterial Agents; D010936:Plant Extracts