Sequence characterized amplified region (SCAR) analysis on DNA from the three medicinal Echinacea species.
In our previous study, RAPD (Random Amplified Polymorphic DNA) analysis revealed species-specific markers for three medicinal Echinacea species (Asteraceae): E. angustifolia DC., E. pallida (Nutt.) Nutt. and E. purpurea (L.) Moench. In the present work, we have converted a RAPD marker (750 bp) for E. purpurea into a SCAR (Sequence Characterized Amplified Region) marker. SCAR-PCR, in fact, revealed the expected amplicon (330 bp) only in E. purpurea and not in the other two species, giving further evidence for differences in medicinal Echinacea spp. genome and confirming a greater similarity between E. pallida and angustifolia.
Adinolfi
Barbara
B
Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, University of Pisa, Italy. badinolfi@yahoo.it
Chicca
Andrea
A
Martinotti
Enrica
E
Breschi
Maria C
MC
Nieri
Paola
P
D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis*; D020900:Echinacea / Q000145:classification / Q000235:genetics*; D006801:Humans; D008517:Phytotherapy; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D018744:DNA, Plant