Analysis of immune cell infiltrates during squamous carcinoma development.
Infiltration of leukocytes into tissue is a common feature of many physiological and pathological conditions. Histopathologically, the diversity of leukocytes that infiltrate a tissue associated with a pathophysiologic response cannot be appreciated and/or examined unless highly selective immunologic detection methods are utilized. Specific populations of infiltrating leukocytes into squamous tissues harboring pre-malignant and/or malignant keratinocytes have recently been demonstrated to play a functionally significant role in the pathogenesis of squamous carcinomas. To evaluate immune cell types and quantify changes in their relative presence and localization during multi-stage neoplastic progression, we performed flow cytometry and histochemical detection using lineage-selective markers. Herein, we provide detailed methodology facilitating these analyses.
Junankar
Simon R
SR
Cancer Research Institute, University of California, San Francisco, San Francisco, California 94143, USA.
Eichten
Alexandra
A
Kramer
Annegret
A
de Visser
Karin E
KE
Coussens
Lisa M
LM
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000276:immunology / Q000473:pathology*; D002465:Cell Movement; D005434:Flow Cytometry; D007113:Immunity, Innate; D007150:Immunohistochemistry; D007962:Leukocytes / Q000502:physiology*; D008264:Macrophages / Q000502:physiology*; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000032:analysis; D012878:Skin Neoplasms / Q000209:etiology / Q000276:immunology / Q000473:pathology*
D016186:Receptor, Macrophage Colony-Stimulating Factor