title: Development of simple and efficient in Planta transformation method for wheat (Triticum aestivum L.) using Agrobacterium tumefaciens.
abstract: Wheat (Triticum aestivum L. var. Shiranekomugi) seeds were soaked in water at 22 degrees C for 1 d. Thereafter, the embryo of the soaked seeds was inoculated with Agrobacterium tumefaciens by piercing a region of the embryonic apical meristem with a needle that had been dipped in an A. tumefaciens inoculum. The inoculated seeds were incubated at 22 degrees C for 2 d and sterilized by cefotaxime (Claforan) (1000 ppm water solution) treatment and then vernalized at 5 degrees C for 25 d. Finally, the seedlings were grown to maturation (T(0) plants) and allowed to pollinate naturally for seed setting (T(1) plants) in pots under nonsterile condition. To examine the transformation by various means, four different strains of A. tumefaciens were used for transformation. The following five lines of evidence proved the transformation: altered phenotype and its transmittance to the next generation, resistance of T(1) seed germination to geneticin or hygromycin B, the detection of a transgene in T(1) plants by PCR analysis and Southern hybridization and the rescue of the plasmid consisting of the integrated T-DNA and flanking wheat genome DNA from T(1) plants. The transformation efficiency of T(1) plants, which were transformed using different A. tumefaciens strains, was estimated to be 33% by PCR analysis, 75% by Southern hybridization and 40% by plasmid rescue.
authors.affiliation: Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano, Japan.
authors.forename: Putu
authors.initials: P
authors.lastname: Supartana
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Shimizu
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nogawa
authors.forename: Hidenari
authors.initials: H
authors.lastname: Shioiri
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nakajima
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Haramoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nozue
authors.forename: Mineo
authors.initials: M
authors.lastname: Kojima
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D012639:Seeds / Q000235:genetics* / Q000382:microbiology; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics*