title: Surface modification of magnetite nanoparticles using lactobionic acid and their interaction with hepatocytes.
abstract: In the current study, superparamagnetic magnetite nanoparticles were surface-modified with lactobionic acid (LA) to improve their intracellular uptake and ability to target hepatocytes. Maltotrionic acid (MA)-modified nanoparticles were also synthesized as a control. Cell culture experiment showed that LA-modified nanoparticles were internalized into hepatocytes and atomic absorption spectrometer (AAS) measurement indicated that the uptake amount of LA-modified magnetite into hepatocytes was higher than that of unmodified and MA-modified nanoparticles. LA-modified nanoparticle solution was injected in rabbit and the magnetic resonance (MR) images obtained showed that LA-coated nanoparticles were selectively accumulated onto the hepatocytes. This result demonstrates that the LA-modified magnetite nanoparticles have a great potential to be used as contrast agent for liver diagnosis.
authors.affiliation: Department of Polymer Science, Kyungpook National University, Puk-gu, Taegu 702-701, South Korea.
authors.forename: K M
authors.initials: KM
authors.lastname: Kamruzzaman Selim
authors.forename: Yong-Soo
authors.initials: YS
authors.lastname: Ha
authors.forename: Sun-Jung
authors.initials: SJ
authors.lastname: Kim
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Chang
authors.forename: Tae-Jeong
authors.initials: TJ
authors.lastname: Kim
authors.forename: Gang
authors.initials: G
authors.lastname: Ho Lee
authors.forename: Inn-Kyu
authors.initials: IK
authors.lastname: Kang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004187:Disaccharides / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D022781:Hepatocytes / Q000737:chemistry / Q000187:drug effects*; D008279:Magnetic Resonance Imaging; D008297:Male; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D010316:Particle Size; D011817:Rabbits; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D004187:Disaccharides; C005608:lactobionic acid; D052203:Ferrosoferric Oxide