[Amplified fragment length polymorphism genotyping of Shigellae and comparison to pulsed-field gel electrophoresis and colicin typing].
Shigella is an etiological agent of communicable and food-borne disease worldwide, so it is important to develop typing for Shigella in epidemiological studies. We compared amplified fragment length polymorphism (AFLP), molecular epidemiological typing, to pulsed-field gel electrophoresis (PFGE) and colicin typing in typeability, reproducibility, discriminatory power, ease of interpretation, and ease of use for 51 Shigella isolates to determine AFLP applicability to Shigella. AFLP showed less reproducibility and ease of interpretation although it was superior to PFGE and colicin typing in typeability and discriminatory power. Specifying the reproducibility of these typing methods, the intrastrain similarity of AFLP was 81.9%-90.5% in each of three strains tested in triplicate trials, while PFGE showed higher similarity, ranging from 92.3%-100%. AFLP created a phylogenetic tree and classified four Shigella species taxonomically, despite its lower reproducibility. These results suggest that AFLP is inferior to PFGE as molecular typing for Shigella epidemiologically in outbreaks or sporadic cases, although AFLP can create a phylogenetic tree for taxonomical purposes.
Noda
Tamie
T
Fukuoka Institute of Health and Environmental Sciences.
Murakami
Koichi
K
Hamasaki
Mitsuhiro
M
Ishiguro
Yasuhisa
Y
Miyahara
Michiko
M
D015373:Bacterial Typing Techniques; D003087:Colicins / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012760:Shigella / Q000145:classification*
D003087:Colicins