title: Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain.
abstract: The ESCRT-I and ESCRT-II complexes help sort ubiquitinated proteins into vesicles that accumulate within multivesicular bodies (MVBs). Crystallographic and biochemical analyses reveal that the GLUE domain of the human ESCRT-II EAP45 (also called VPS36) subunit is a split pleckstrin-homology domain that binds ubiquitin along one edge of the beta-sandwich. The structure suggests how human ESCRT-II can couple recognition of ubiquitinated cargoes and endosomal phospholipids during MVB protein sorting.
authors.affiliation: Department of Biochemistry, University of Utah, Salt Lake City, Utah 84112-5650, USA.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Alam
authors.forename: Charles
authors.initials: C
authors.lastname: Langelier
authors.forename: Frank G
authors.initials: FG
authors.lastname: Whitby
authors.forename: Sajjan
authors.initials: S
authors.lastname: Koirala
authors.forename: Howard
authors.initials: H
authors.lastname: Robinson
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Hill
authors.forename: Wesley I
authors.initials: WI
authors.lastname: Sundquist
mesh_terms: D020816:Amino Acid Motifs; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray; D056827:Endosomal Sorting Complexes Required for Transport; D011992:Endosomes / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D022161:Transport Vesicles / Q000378:metabolism*; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D011993:Recombinant Fusion Proteins; D025801:Ubiquitin; C516152:VPS36 protein, human; D033921:Vesicular Transport Proteins