Sensitive and species-specific detection of Clonorchis sinensis by PCR in infected snails and fishes.
In the present study, PCR procedures have been established for a rapid and easy preparation of DNA of parasite stages from the intermediate hosts, i.e. sporocysts and rediae in snails and metacercariae in fishes. Primers have been developed, which enable a highly sensitive and species-specific detection of Clonorchis sinensis.
Müller
Boris
B
Department of Zoomorphology, Cell Biology and Parasitology, Heinrich-Heine University Duesseldorf, Dusseldorf, Germany.
Schmidt
Jürgen
J
Mehlhorn
Heinz
H
D000818:Animals; D003004:Clonorchis sinensis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005399:Fishes / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012908:Snails / Q000469:parasitology*; D013045:Species Specificity