Characterization of the Rana grylio virus 3beta-hydroxysteroid dehydrogenase and its novel role in suppressing virus-induced cytopathic effect.
The 3beta-hydroxysteroid dehydrogenase (3beta-HSD) isoenzymes play a key role in cellular steroid hormone synthesis. Here, a 3beta-HSD gene homolog was cloned from Rana grylio virus (RGV), a member of family Iridoviridae. RGV 3beta-HSD gene has 1068bp, encoding a 355aa predicted protein. Transcription analyses showed that RGV 3beta-HSD gene was transcribed immediate-early during infection from an initiation site 19 nucleotides upstream of the translation start site. Confocal microscopy revealed that the 3beta-HSD-EGFP fusion protein was exclusively colocalized with the mitochondria marker (pDsRed2-Mito) in EPC cells. Upon morphological observation and MTT assay, it was revealed that overexpression of RGV 3beta-HSD in EPC cells could apparently suppress RGV-induced cytopathic effect (CPE). The present studies indicate that the RGV immediate-early 3beta-HSD gene encodes a mitochondria-localized protein, which has a novel role in suppressing virus-induced CPE. All these suggest that RGV 3beta-HSD might be a protein involved in host-virus interaction.
Sun
Wei
W
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate University of Chinese Academy of Sciences, Wuhan 430072, China.
Huang
Youhua
Y
Zhao
Zhe
Z
Gui
Jianfang
J
Zhang
Qiya
Q
D000595:Amino Acid Sequence; D002277:Carcinoma / Q000473:pathology / Q000821:virology*; D045744:Cell Line, Tumor; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000737:chemistry* / Q000378:metabolism*; D007497:Iridoviridae / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D014779:Virus Replication / Q000502:physiology*
D006913:Hydroxysteroid Dehydrogenases