Multipotent adult progenitor cell isolation and culture procedures.
We describe methods for isolation of multipotent adult progenitor cells (MAPCs) from newborn to 6-week-old mice and rats. The maintenance of these cells, including their culture, media formulas, and quality control procedures, are also explained. Additionally, ways to identify MAPCs including their phenotype and morphology are discussed.
Breyer
April
A
University of Minnesota, Stem Cell Institute, Minneapolis, MN 55455, USA.
Estharabadi
Nima
N
Oki
Masayuki
M
Ulloa
Fernando
F
Nelson-Holte
Molly
M
Lien
Laura
L
Jiang
Yuehua
Y
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D005615:Freezing; D007150:Immunohistochemistry; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D010641:Phenotype; D011786:Quality Control; D051381:Rats; D014021:Tissue Preservation
D003470:Culture Media