title: Immunoproteasomes are essential for clearance of Listeria monocytogenes in nonlymphoid tissues but not for induction of bacteria-specific CD8+ T cells.
abstract: Microbial infections induce the replacement of constitutive proteasomes by immunoproteasomes (I-proteasomes). I-proteasomes support efficient generation of MHC class I epitopes and influence immunodominance hierarchies of CD8(+) T cells. Recently, the function of I-proteasomes in antimicrobial responses was challenged by showing that the lack of I-proteasomes has no effect on induction and function of lymphocytic choriomeningitis virus-specific CD8(+) T cells. Here, we show that infection with Listeria monocytogenes rapidly induces I-proteasomes in nonlymphoid tissues, which leads to enhanced generation of protection relevant CD8(+) T cell epitopes. I-proteasome-deficient mice (beta5i(-/-) mice) exhibited normal frequencies of L. monocytogenes-specific CD8(+) T cells. However, clearance of L. monocytogenes in liver but not spleen was significantly impaired in I-proteasome-deficient mice. In summary, our studies demonstrate that induction of I-proteasomes is required for CD8(+) T cell-mediated elimination of L. monocytogenes from nonlymphoid but not lymphoid tissues.
authors.affiliation: Institut für Biochemie, Charité-Universitätsmedizin, Berlin, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Strehl
authors.forename: Thorsten
authors.initials: T
authors.lastname: Joeris
authors.forename: Melanie
authors.initials: M
authors.lastname: Rieger
authors.forename: Alexander
authors.initials: A
authors.lastname: Visekruna
authors.forename: Kathrin
authors.initials: K
authors.lastname: Textoris-Taube
authors.forename: Stefan H E
authors.initials: SH
authors.lastname: Kaufmann
authors.forename: Peter-Michael
authors.initials: PM
authors.lastname: Kloetzel
authors.forename: Ulrike
authors.initials: U
authors.lastname: Kuckelkorn
authors.forename: Ulrich
authors.initials: U
authors.lastname: Steinhoff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003106:Colon / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D004847:Epithelial Cells / Q000201:enzymology / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000378:metabolism*; D022781:Hepatocytes / Q000201:enzymology / Q000276:immunology; D008089:Listeria monocytogenes; D008088:Listeriosis / Q000201:enzymology / Q000276:immunology*; D008099:Liver / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology; D008213:Lymphocyte Activation; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000201:enzymology / Q000276:immunology / Q000382:microbiology
chemical_list: D018984:Epitopes, T-Lymphocyte; D046988:Proteasome Endopeptidase Complex