title: [Determination of saponin I in extracts of Allium macrostemnon].
abstract: OBJECTIVE
  To develop an HPLC method for determination of saponin I in bulb extracts of Allium macrostemon.
  
  
  METHODS
  The extract was refluxed by methanol and then filtrated. The filtrate was concentrated and the bioactive saponin was measured by HPLC-RID.
  
  
  RESULTS
  The calibration curve was in good linearity over the range of 5-100 microg with the regression equation of Y= 9.35 x 10(4) X - 2.78 x 10(4) (r = 0.9998) and the average recovery was 101.1% (n = 5, RSD 1.6%).
  
  
  CONCLUSIONS
  The method is simple, quick and reproducible and can be applied in the quality control of A. macrostemon.
authors.affiliation: Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Hai-feng
authors.initials: HF
authors.lastname: Chen
authors.forename: Nai-li
authors.initials: NL
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Dai
authors.forename: Xin-sheng
authors.initials: XS
authors.lastname: Yao
mesh_terms: D000490:Allium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D013261:Sterols / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D012503:Saponins; D013261:Sterols