[Blocking the gastrin/cholecystokinin-B receptor autocrine loop influences the growth and apoptosis of human gastric cancer cells].
OBJECTIVE
To study whether gastrin/cholecystokinin-B (CCK-B) receptor autocrine loop exists in human gastric cancer cells and the effects of gastrin/CCK-B receptor autocrine loop on the growth and apoptosis of human gastric cancer cells.


METHODS
Human gastric cancer cells of the line SGC-7901 were cultured. The expression of gastrin and CCK-B was detected by RT-PCR, immunocytochemistry and radioimmunoassay. Antibody against gastrin was added so as to block the autocrine loop to observe the growth rate, cell cycle and apoptosis by 3-(4.5-dimethylthiazol-z-yl) -2, 5-diphenyl tertrazolium blue (MMT) colorimetric assay and flow cytometry (FCM).


RESULTS
The SGC-7901 cells co-expressed gastrin and CCK-B receptor mRNAs, confirmed by sequencing, thus forming gastrin/the CCK-B receptor autocrine loop. After the blocking of the autocrine loop by antibody against gastrin, the cell growth rate and the number of cells residing in the S-phase of the cell cycle decreased and the apoptotic rate increased in an antibody concentration-dependent manner.


CONCLUSIONS
Human gastric cancer SGC-7901 cells possess the gastrin/the CCK-B receptor autocrine loop. Blocking the autocrine loop inhibits the cell proliferation and promotes the cell apoptosis.
Wang
Wen-ling
WL
Department of Medical Oncology, Molecular Biology Laboratory, Hospital affiliated to Guiyang Medical College, Guiyang 550004, China.
Zhou
Jian-jiang
JJ
Zhou
Xi-chun
XC
Yang
Xiao-feng
XF
D000183:Actihaemyl; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D019898:Autocrine Communication; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005755:Gastrins / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D043887:Receptor, Cholecystokinin B / Q000096:biosynthesis*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*
D000911:Antibodies, Monoclonal; D005755:Gastrins; D012333:RNA, Messenger; D043887:Receptor, Cholecystokinin B; D000183:Actihaemyl