[Determination of indirubin and indigo in Baphicacanthus cusia (Nees) Bremek by HPLC].
OBJECTIVE
To establish a method for content determination of indirubin and indigo in Baphicacanthus cusia (Nees) Bremek.


METHODS
HPLC was used with C18 column, methanol-water (75:25) as mobile phase and detecting wavelength 289 nm.


RESULTS
The range linear curve was 3.875 approximately 23.25 microg/ml (indirubin), 3.05 approximately 18.3 microg/ml (indigo). The average recovery was 96.9%, RSD =3.5%, n =5 (indirubin); 96.9% RSD = 2.8%, n = 5 (indigo).


CONCLUSIONS
The method is proper for the quality control of Baphicacanthus cusia (Nees) Bremek.
Hou
Hui-chan
HC
Guangzhou Institute for Drug Control, China.
Liang
Shao-zhen
SZ
D027901:Acanthaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D007203:Indigo Carmine; D007211:Indoles / Q000032:analysis* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011208:Powders; D011786:Quality Control; D012621:Seasons
D004365:Drugs, Chinese Herbal; D007211:Indoles; D011208:Powders; D007203:Indigo Carmine; C027185:indirubin