[Utility of anti-cytomegalovirus (CMV) pp65 antibody and cytology in CMV antigenemia and CMV disease diagnosis].
We studied cytomegalovirus (CMV) pp65 positive cells (pp65+ cells) in 275 peripheral blood (PB) samples obtained from 157 patients with clinical symptoms such as fever and diarrhea and in 8 biopsy specimens diagnosed as CMV disease immunohistochemically and immunocytologically using the anti-CMV pp65 antibody. We compared our results to those of the clinical inspection center using 76 samples. pp65+ cells was detected in 164 of 275 samples by our method, and number of pp65+ cells in 83 samples was less than 10. The comparison showed a strong correlation between the two results (r = 0.844). Fewer than 10 pp65+ cells were detected in 21 samples by our method, but no pp 65+ cell was found in 18 of the 21 samples (85.7%) by the clinical inspection center. We concluded that we detected a small number of pp65+ cell because we examined a large number of cells. Many pp65+ cell that did not show an owl's eye configuration were observed in biopsy specimens. Data suggested that cytology and immunostaining using anti-CMV pp65 antibody are useful in the detection of pp65+ cells in PB and biopsy specimens.
Emura
Iwao
I
The Department of Surgical Pathology, Nagaoka Red Cross Hospital.
D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D006801:Humans
D000914:Antibodies, Viral; D000956:Antigens, Viral