title: [Determination of three kind of diester diterpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products by HPLC].
abstract: OBJECTIVE
  To establish an HPLC method for the determination of three kind of diester diterpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products namely mesaconitine, aconitine and hypaconitine.
  
  
  METHODS
  A Zorbax Eclipse XDB C18 column was used, and ammonium acetate solution-acetonitrile as the mobile phase, with a flow rate of 1.0 mL x min(-1) and a detection wavelength of 230 nm.
  
  
  RESULTS
  Mesaconitine, aconitine and hypaconitine, were separated, and the calibration curves of them were in good linearity over the range of 0.035 7-1.784 microg (r = 0.9999), 0.0126-0.632 microg (r = 0.9997) and 0.0334-1.672 microg (r = 0.9997) respectively.
  
  
  CONCLUSIONS
  This method is simple and accurate. It can be used in the identification and quality control of A. carmichaeli.
authors.affiliation: Department of Chinese Pharmacy, Beijing University of Traditional Chinese Medicine, Beifing 100102, China. fang68-8@hotmail.com
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Xiang-Hong
authors.initials: XH
authors.lastname: Yu
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Yu-Yuan
authors.initials: YY
authors.lastname: Tan
authors.forename: Yan-Jiang
authors.initials: YJ
authors.lastname: Qiao
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives / Q000032:analysis; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006358:Hot Temperature; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D000470:Alkaloids; C058309:hypaconitine; C019470:mesaconitine; D000157:Aconitine