title: Dynamic nuclear actin assembly by Arp2/3 complex and a baculovirus WASP-like protein.
abstract: Diverse bacterial and viral pathogens induce actin polymerization in the cytoplasm of host cells to facilitate infection. Here, we describe a pathogenic mechanism for promoting dynamic actin assembly in the nucleus to enable viral replication. The baculovirus Autographa californica multiple nucleopolyhedrovirus induced nuclear actin polymerization by translocating the host actin-nucleating Arp2/3 complex into the nucleus, where it was activated by p78/83, a viral Wiskott-Aldrich syndrome protein (WASP)-like protein. Nuclear actin assembly by p78/83 and Arp2/3 complex was essential for viral progeny production. Recompartmentalizing dynamic host actin may represent a conserved mode of pathogenesis and reflect viral manipulation of normal functions of nuclear actin.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
authors.forename: Erin D
authors.initials: ED
authors.lastname: Goley
authors.forename: Taro
authors.initials: T
authors.lastname: Ohkawa
authors.forename: Joel
authors.initials: J
authors.lastname: Mancuso
authors.forename: Jeffrey B
authors.initials: JB
authors.lastname: Woodruff
authors.forename: Joseph A
authors.initials: JA
authors.lastname: D'Alessio
authors.forename: W Zacheus
authors.initials: WZ
authors.lastname: Cande
authors.forename: Loy E
authors.initials: LE
authors.lastname: Volkman
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Welch
mesh_terms: D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000818:Animals; D001704:Biopolymers / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching; D009036:Moths; D009154:Mutation; D019251:Nucleocapsid / Q000378:metabolism / Q000648:ultrastructure; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014771:Virion / Q000648:ultrastructure; D014779:Virus Replication; D051316:Wiskott-Aldrich Syndrome Protein / Q000737:chemistry
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D001704:Biopolymers; D014764:Viral Proteins; D051316:Wiskott-Aldrich Syndrome Protein