Identification of genes from the obligate intracellular plant pathogen, Plasmodiophora brassicae.
Plasmodiophora brassicae is an intracellular pathogen that infects plants in the Brassicaceae family. Although an important pathogen group, information on the genomic makeup of the plasmodiophorids is almost completely lacking. We performed suppression subtractive hybridization (SSH) between RNA from P. brassicae-infected and uninfected Arabidopsis tissue, then screened 232 clones from the resulting SSH library. In addition, we used an oligo-capping procedure to screen 305 full-length cDNA clones from the infected tissue. A total of 76 new P. brassicae gene sequences were identified, the majority of which were extended to full length at the 5' end by the use of RACE amplification. Many of the unisequences were predicted to contain signal peptides for ER translocation. Although we located few sequences in total, these markedly increase available data from the plasmodiophorids, and provide new opportunities to examine plasmodiophorid biology. Our study also points towards the best methods for future plasmodiophorid gene discovery.
Bulman
Simon
S
National Centre for Advanced Bio-Protection Technologies, Lincoln University, Canterbury, New Zealand. bulmans@crop.cri.nz
Siemens
Johannes
J
Ridgway
Hayley J
HJ
Eady
Colin
C
Conner
Anthony J
AJ
D001937:Brassica / Q000382:microbiology*; D003001:Cloning, Molecular; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D009693:Nucleic Acid Hybridization / Q000379:methods