LEF-1 is crucial for neutrophil granulocytopoiesis and its expression is severely reduced in congenital neutropenia.
We demonstrate here that lymphoid enhancer-binding factor 1 (LEF-1) mediates the proliferation, survival and differentiation of granulocyte progenitor cells. We initially documented the importance of this transcription factor in the bone marrow of individuals with severe congenital neutropenia (CN) with a 'differentiation block' at the promyelocytic stage of myelopoiesis. LEF-1 expression was greatly reduced or even absent in CN arrested promyelocytes, resulting in defective expression of the LEF-1 target genes CCND1, MYC and BIRC5, encoding cyclin D1 (ref. 2), c-Myc and survivin, respectively. In contrast, healthy individuals showed highest LEF-1 expression in promyelocytes. Reconstitution of LEF-1 in early hematopoietic progenitors of two individuals with CN corrected the defective myelopoiesis and resulted in the differentiation of these progenitors into mature granulocytes. Repression of endogenous LEF-1 by specific short hairpin RNA inhibited proliferation and induced apoptosis of CD34(+) progenitors from healthy individuals and of cells from two myeloid lines (HL-60 and K562). C/EBPalpha, a key transcription factor in granulopoiesis, was directly regulated by LEF-1. These observations indicate that LEF-1 is an instructive factor regulating neutrophilic granulopoiesis whose absence plays a critical role in the defective maturation program of myeloid progenitors in individuals with CN.
Skokowa
Julia
J
Department of Pediatric Hematology and Oncology Carl-Neuberg-Str. 1, 30625 Hannover, Germany. skokowa.julia@mh-hannover.de
Cario
Gunnar
G
Uenalan
Murat
M
Schambach
Axel
A
Germeshausen
Manuela
M
Battmer
Karin
K
Zeidler
Cornelia
C
Lehmann
Ulrich
U
Eder
Matthias
M
Baum
Christopher
C
Grosschedl
Rudolf
R
Stanulla
Martin
M
Scherr
Michaela
M
Welte
Karl
K
D015703:Antigens, CD / Q000096:biosynthesis; D018952:Antigens, CD34 / Q000096:biosynthesis; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis; D001483:Base Sequence; D019938:Cyclin D1 / Q000096:biosynthesis; D005786:Gene Expression Regulation; D006098:Granulocytes / Q000166:cytology* / Q000378:metabolism; D018922:HL-60 Cells; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D020014:K562 Cells; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D009503:Neutropenia / Q000151:congenital* / Q000473:pathology*; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D063268:Sialic Acid Binding Ig-like Lectin 3
D015703:Antigens, CD; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; C497573:LEF1 protein, human; D051821:Lymphoid Enhancer-Binding Factor 1; D016271:Proto-Oncogene Proteins c-myc; D063268:Sialic Acid Binding Ig-like Lectin 3; D019938:Cyclin D1