title: The use of DNA microarray technology for detection and genetic characterisation of chlamydiae.
abstract: Due to its highly parallel approach, DNA microarray technology opens up new possibilities that may be particularly beneficial for laboratory diagnosis of infectious diseases. We developed a microarray assay for detection and differentiation of all currently defined chlamydial species belonging to the genera Chlamydia and Chlamydophila using the ArrayTube system, which we found to be particularly user-friendly and economical. The test includes PCR amplification of a 23S rDNA target region with concurrent biotinylation and subsequent hybridisation in the ArrayTube, a micro-reaction tube carrying the microarray chip on the bottom. In addition to high specificity, the assay was shown to allow detection and genetic characterisation of single PCR-amplifiable target DNA copies.
authors.affiliation: Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Bacterial Infections and Zoonoses, Jena, Germany. konrad.sachse@fli.bund.de
authors.forename: K
authors.initials: K
authors.lastname: Sachse
authors.forename: H
authors.initials: H
authors.lastname: Hotzel
authors.forename: P
authors.initials: P
authors.lastname: Slickers
authors.forename: R
authors.initials: R
authors.lastname: Ehricht
mesh_terms: D002689:Chlamydia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D002690:Chlamydia Infections / Q000175:diagnosis / Q000382:microbiology; D023501:Chlamydophila / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D023521:Chlamydophila Infections / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity