title: Plasma levels of t-PA and PAI-1 correlate with the formation of experimental post-surgical peritoneal adhesions.
abstract: This study has evaluated whether systemic changes of plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) parallel the adhesions development and whether they could be used as predictors of adhesion risk. This has been studied in an animal model of post-surgical peritoneal adhesion by monitoring for 10 days the plasma and tissue levels of t-PA and PAI-1. The results showed that both tissular and plasmatic levels of t-PA were decreased in concomitance with the development of peritoneal adhesions. In contrast, PAI-1 was found increased into the tissue and into the plasma samples of the rats taken at 5 and 10 days time points. Inflammatory mediators such as ICAM-1, VCAM-1, and IL-6 within the peritoneal lavage fluid also correlated with the adhesion formation process. In conclusion, post-surgical peritoneal adhesions provide alterations of local inflammatory components and local and systemic fibrinolytic components, possibly with PAI-1 quenching t-PA. This may have potential for the identification of high-risk patients.
authors.affiliation: Section of Pharmacology L Donatelli, Department of Experimental Medicine, Second University of Naples, Naples, Italy.
authors.forename: Clara
authors.initials: C
authors.lastname: Di Filippo
authors.forename: Alessandro
authors.initials: A
authors.lastname: Falsetto
authors.forename: Vito
authors.initials: V
authors.lastname: De Pascale
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Tufariello
authors.forename: Domenico
authors.initials: D
authors.lastname: De Lucia
authors.forename: Francesco
authors.initials: F
authors.lastname: Rossi
authors.forename: Michele
authors.initials: M
authors.lastname: D'Amico
authors.forename: Antonio
authors.initials: A
authors.lastname: Cennamo
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D010532:Peritoneal Diseases / Q000097:blood* / Q000601:surgery; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood*; D010960:Plasminogen Activators / Q000097:blood*; D011237:Predictive Value of Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D010960:Plasminogen Activators