[Multiple intracerebral lesions. Identification of the causative agent by 16S rDNA-PCR].
Molecular biological methods such as polymerase chain reaction (PCR) enable microbiologists to detect bacteria even if antibiotic treatment has already been started. Based on this case report of a 39-year-old man with multiple intracerebral lesions, we describe a PCR method called 16S rDNA-PCR which can be used to identify panbacterial DNA by focussing on the universal gene sequences for the bacterial 16S part of the ribosome.
Boden
K
K
Institut für Medizinische Mikrobiologie, Klinikum der Friedrich-Schiller-Universität, Semmelweisstrasse 4, 07743 Jena, Germany. KATHARINA.Boden@med.uni-jena.de
Joachimski
F
F
Ewald
C
C
Behrendt
W
W
Baier
M
M
Schmidt
K-H
KH
Straube
E
E
D000328:Adult; D001921:Brain / Q000473:pathology; D001922:Brain Abscess / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal; D003937:Diagnosis, Differential; D005674:Fusobacterium Infections / Q000175:diagnosis* / Q000382:microbiology; D016967:Fusobacterium nucleatum / Q000235:genetics*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S