title: Quantitative proteome analysis using D-labeled N-ethylmaleimide and 13C-labeled iodoacetanilide by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: A new methodology for quantitative analysis of proteins is described, applying stable-isotope labeling by small organic molecules combined with one- or two-dimensional electrophoresis and MALDI-TOF-MS, also allowing concurrent protein identification by peptide mass fingerprinting. Our method eliminates fundamental problems in other existing isotope-tagging methods requiring liquid chromatography and MS/MS, such as isotope effects, fragmentation, and solubility. It is also anticipated to be more practical and accessible than those LC-dependent methods.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA.
authors.forename: Sadamu
authors.initials: S
authors.lastname: Kurono
authors.forename: Tamie
authors.initials: T
authors.lastname: Kurono
authors.forename: Naoka
authors.initials: N
authors.lastname: Komori
authors.forename: Satomi
authors.initials: S
authors.lastname: Niwayama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D002247:Carbon Isotopes / Q000737:chemistry*; D003903:Deuterium; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005033:Ethylmaleimide / Q000737:chemistry*; D019476:Insect Proteins / Q000032:analysis*; D007460:Iodoacetamide / Q000737:chemistry*; D007553:Isotope Labeling; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002247:Carbon Isotopes; D019476:Insect Proteins; D020543:Proteome; D003903:Deuterium; D005033:Ethylmaleimide; D007460:Iodoacetamide