Detection of herpes simplex virus type 1 DNA in bilateral human trigeminal ganglia and optic nerves by polymerase chain reaction.
Herpes simplex virus type 1 (HSV-1) DNA was extracted from human trigeminal ganglia of 121 cadavers aged between 3 months and 94 years, and its PCR amplification was performed for the RL2 HSV-1 sequence using two pairs of primers. The HSV-1 DNA was detected in 74 of 121 patients (61%); 70/74 bilaterally, 3/74 only on the left side, and 1/74 only on the right side. Although the PCR-positive rate was significantly higher in advanced age, the correlation between the PCR-positive rate and gender was unclear. Additionally, HSV-1 DNA was not detected in any of the 50 optic nerve samples.
Motani
Hisako
H
Department of Legal Medicine, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba, Japan. hms1466@faculty.chiba-u.jp
Sakurada
Koichi
K
Ikegaya
Hiroshi
H
Akutsu
Tomoko
T
Hayakawa
Mutsumi
M
Sato
Yayoi
Y
Yajima
Daisuke
D
Sato
Kaoru
K
Kobayashi
Kazuhiro
K
Iwase
Hirotaro
H
D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D002102:Cadaver; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006561:Herpes Simplex / Q000453:epidemiology* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009900:Optic Nerve / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017678:Sex Distribution; D012668:Trigeminal Ganglion / Q000821:virology*
D004279:DNA, Viral