A mouse homologue of Strawberry Notch is transcriptionally regulated by Reelin signal.
Reelin is a glycoprotein secreted by specific neuronal populations of the adult and developing nervous system of vertebrates. The morphological abnormalities in the brain of reeler, the Reelin deficient mutant mice, indicate that Reelin is essential for the brain morphogenesis. However, biochemical function of Reelin signal is not well understood. Here, we examined possible function of Reelin signal in regulation of gene expression by performing a microarray analysis. We found that expression level of a mouse homologue of Strawberry Notch (mSno1) is markedly reduced in the reeler embryos. In situ hybridization showed that mSno1 is expressed in the developing nervous system colocalizing with expression of ApoER2, a Reelin receptor. Treatment of P19 cells with Reelin protein enhanced mSno1 expression. Overexpression of ApoER2 with Reelin treatment gave a synergistic effect on mSno1 expression level. These observations suggest that Reelin signal is involved in embryonic expression of a novel vertebrate gene, mSno1.
Baba
Kousuke
K
Department of Anatomy and Developmental Neurobiology, Kobe University School of Medicine, Kobe 650-0017, Japan.
Dekimoto
Hideyuki
H
Muraoka
Daisuke
D
Agata
Kiyokazu
K
Terashima
Toshio
T
Katsuyama
Yu
Y
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics; D000091042:Reelin Protein; D050436:Regulatory Elements, Transcriptional / Q000235:genetics*; D012697:Serine Endopeptidases / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*
D015816:Cell Adhesion Molecules, Neuronal; D029721:Drosophila Proteins; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D020033:Protein Isoforms; D000091042:Reelin Protein; D014157:Transcription Factors; C106204:sno protein, Drosophila; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases