[Association of abnormal cyclooxygenase-2 gene expression with colorectal carcinoma metastasis].
OBJECTIVE
To investigate cyclooxygenase-2 (COX-2) expression of in colorectal carcinoma cell lines and tissues and its clinical implications.


METHODS
SP immunohistochemistry was used to detect COX-2 protein in SW480 and SW620 cell lines and 50 primary colorectal carcinoma and 50 lymphoma metastasis carcinoma specimens. Real-time PCR was used to detect COX-2 mRNA expression in SW480 and SW620 cell lines.


RESULTS
SW480 and SW620 cell lines both highly expressed COX-2. The positive expression levels of COX-2 increased significantly in lymph node metastatic carcinoma in comparison with primary colorectal carcinoma (P<0.05). The expression COX-2 mRNA in SW620 cell line was higher than that of SW480 cell line, showing a mean expression increment of 2.268 folds in SW620 cell line relative to SW480.


CONCLUSIONS
Elevated COX-2 expression may be associated with lymph node metastases of colorectal carcinoma.
Li
Zu-guo
ZG
Key Laboratory of Functional Proteomics and Transcriptomics of Main Human Diseases of Guangdong Province and Ministry of Education, Department of Pathology, Nangfang Hospital, Southern Medical University, Guangzhou 510515, China. Lizg@fimmu.com
Liu
Teng-fei
TF
Xie
Wei-bing
WB
Zhou
Jun
J
Yu
Li
L
Ding
Yan-qing
YQ
D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D051546:Cyclooxygenase 2