title: Expression, purification, crystallization, and preliminary X-Ray analysis of the human UDP-glucose dehydrogenase.
abstract: UDP-glucose dehydrogenase (UGDH) catalyzes the synthesis of UDP-glucuronic acid from UDP-glucose resulting in the formation of proteoglycans that are involved in promoting normal cellular growth and migration. Overproduction of proteoglycans has been implicated in the progression of certain epithelial cancers. Here, human UGDH (hUGDH) was purified and crystallized from a solution of 0.2 M ammonium sulfate, 0.1 M Na cacodylate, pH 6.5, and 21% PEG 8000. Diffraction data were collected to a resolution of 2.8 A. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1) with unit-cell parameters a = 173.25, b = 191.16, c = 225.94 A, and alpha = beta = gamma = 90.0 degrees. Based on preliminary analysis of the diffraction data, we propose that the biological unit of hUGDH is a tetramer.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 138-736, South Korea.
authors.forename: Jae Wan
authors.initials: JW
authors.lastname: Huh
authors.forename: Robert Charles
authors.initials: RC
authors.lastname: Robinson
authors.forename: Han Sam
authors.initials: HS
authors.lastname: Lee
authors.forename: Jae Il
authors.initials: JI
authors.lastname: Lee
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Heo
authors.forename: Hyun Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Han
authors.initials: H
authors.lastname: Choe
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D006801:Humans; D014532:Uridine Diphosphate Glucose / Q000378:metabolism; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D014533:Uridine Diphosphate Glucose Dehydrogenase; D014532:Uridine Diphosphate Glucose