Modulation of talA gene in pentose phosphate pathway for overproduction of poly-beta-hydroxybutyrate in transformant Escherichia coli harboring phbCAB operon.
The talA gene encoding transaldolase, the key enzyme in the nonoxidative pentose phosphate pathway, was amplified in a transformant Escherichia coli harboring the phbCAB operon to shift the metabolic flux of the hexose mono-phosphate shunt to the odd-ball biosynthesis pathway for poly-beta-hydroxybutyrate overproduction. The PHB content in the transformant E. coli coharboring the phbCAB operon and talA gene increased from 28.2% to 52.3%, and the retarded cell growth was overcome. This increase seems to be mainly due to the concomitant supplies of the intermediates NADPH and acetyl-CoA, which are from the activated pentose phosphate pathway through the modulation of the talA gene and from the Embden-Meyerhof pathway.
Song
Byoung-Geun
BG
Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu, S Korea.
Kim
Tae-Kwon
TK
Jung
Young-Mi
YM
Lee
Yong-Hyun
YH
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D020118:Cupriavidus necator / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D006885:Hydroxybutyrates / Q000378:metabolism*; D009876:Operon / Q000235:genetics*; D010427:Pentose Phosphate Pathway / Q000235:genetics*; D011091:Polyesters / Q000378:metabolism*; D014153:Transaldolase / Q000096:biosynthesis / Q000235:genetics*; D000094122:Polyhydroxybutyrates
D001426:Bacterial Proteins; D006885:Hydroxybutyrates; D011091:Polyesters; D014153:Transaldolase; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates