Differences in activities of the enzymes of nucleotide metabolism and its implications for cardiac xenotransplantation.
Xenotransplantation is one be possible solution for a severe shortage of human organs available for transplantation. However, only a few studies addressed metabolic compatibility of transplanted animal organs. Our aim was to compare activities of adenosine metabolizing enzymes in the heart of different species that are relevant to clinical or experimental xenotransplantation. We noted fundamental differences: ecto-5' nucleotidease (E5' N) activity was 4-fold lower in pig and baboon hearts compared to the human hearts while mouse activity was compatible with human and rat activity was three times higher than human. There also were significant differences in AMP-deaminase (AMPD), adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) activities. We conclude that differences in nucleotide metabolism may contribute to organ dysfunction after xenotransplantation.
Yuen
A H Y
AH
Heart Science Centre, Imperial College at Harefield Hospital, Harefield, UK.
Khalpey
Z
Z
Lavitrano
M
M
McGregor
C G A
CG
Kalsi
K K
KK
Yacoub
M H
MH
Smolenski
R T
RT
D015720:5'-Nucleotidase / Q000096:biosynthesis; D000659:AMP Deaminase / Q000096:biosynthesis; D000241:Adenosine / Q000737:chemistry; D000243:Adenosine Deaminase / Q000096:biosynthesis; D000818:Animals; D016027:Heart Transplantation / Q000379:methods*; D006801:Humans; D051379:Mice; D009711:Nucleotides / Q000737:chemistry*; D010215:Papio; D011683:Purine-Nucleoside Phosphorylase / Q000096:biosynthesis; D051381:Rats; D013045:Species Specificity; D013552:Swine; D014183:Transplantation, Heterologous / Q000379:methods*
D009711:Nucleotides; D011683:Purine-Nucleoside Phosphorylase; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase; D000659:AMP Deaminase; D000241:Adenosine