title: Targeted delivery of antibody conjugated liposomal drug carriers to rat myocardial infarction.
abstract: Immunoliposome (IL) targeting to areas of inflammation after an acute myocardial infarction (MI) could provide the means by which pro-angiogenic compounds can be selectively targeted to the infarcted region. The adhesion of model drug carriers and ILs coated with an antibody to P-selectin was quantified in a rat model of MI following left coronary artery ligation. Anti-P-selectin coated model drug carriers showed a 140% and 180% increase in adhesion in the border zone of the MI 1 and 4 h post-MI, respectively. Radiolabeled anti-P-selectin ILs injected immediately post-MI and allowed to circulate 24 h showed an 83% increase in targeting to infarcted myocardium when compared to adjacent non-infarcted myocardium. Radiolabeled anti-P-selectin ILs injected 4 h post-MI and allowed to circulate for 24 h showed a 92% increase in accumulation in infarcted myocardium when compared to adjacent non-infarcted myocardium. Targeting to upregulated adhesion molecules on the endothelium provides a promising strategy for selectively delivering compounds to the infarct region of the myocardium using our liposomal-based drug delivery vehicle.
authors.affiliation: Department of Mechanical Engineering, Temple University, 1947 North, 12th Street, Philadelphia, PA 19122, USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Scott
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Ramakrishna
authors.initials: R
authors.lastname: Nallamothu
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Pattillo
authors.forename: Georgina
authors.initials: G
authors.lastname: Perez-Liz
authors.forename: Andrew
authors.initials: A
authors.lastname: Issekutz
authors.forename: Luis
authors.initials: L
authors.lastname: Del Valle
authors.forename: George C
authors.initials: GC
authors.lastname: Wood
authors.forename: Mohammad F
authors.initials: MF
authors.lastname: Kiani
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000138:chemical synthesis / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy; D008081:Liposomes; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy*; D009206:Myocardium / Q000276:immunology* / Q000473:pathology; D019007:P-Selectin / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D008081:Liposomes; D019007:P-Selectin