title: The first potassium channel toxin from the venom of the Iranian scorpion Odonthobuthus doriae.
abstract: The very first member of K(+) channels toxins from the venom of the Iranian scorpion Odonthobuthus doriae (OdK1) was purified, sequenced and characterized physiologically. OdK1 has 29 amino acids, six conserved cysteines and a pI value of 4.95. Based on multiple sequence alignments, OdK1 was classified as alpha-KTx 8.5. The pharmacological effects of OdK1 were studied on six different cloned K(+) channels (vertebrate Kv1.1-Kv1.5 and Shaker IR) expressed in Xenopus laevis oocytes. Interestingly, OdK1 selectively inhibited the currents through Kv1.2 channels with an IC50 value of 183+/-3 nM but did not affect any of the other channels.
authors.affiliation: Laboratory of Toxicology, University of Leuven, Onderwijs and Navorsing II, Herestraat 49, P.O. Box 922, 3000 Leuven, Belgium.
authors.forename: Yousra
authors.initials: Y
authors.lastname: Abdel-Mottaleb
authors.forename: Elke
authors.initials: E
authors.lastname: Clynen
authors.forename: Amir
authors.initials: A
authors.lastname: Jalali
authors.forename: Frank
authors.initials: F
authors.lastname: Bosmans
authors.forename: Hossein
authors.initials: H
authors.lastname: Vatanpour
authors.forename: Liliane
authors.initials: L
authors.lastname: Schoofs
authors.forename: Jan
authors.initials: J
authors.lastname: Tytgat
mesh_terms: D000818:Animals; D004594:Electrophysiology; D020128:Inhibitory Concentration 50; D009865:Oocytes; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000187:drug effects* / Q000502:physiology; D012604:Scorpion Venoms / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D016415:Sequence Alignment; D014162:Transfection; D014982:Xenopus laevis
chemical_list: C515957:K1 toxin, Odonthobuthus doriae; D015221:Potassium Channels; D012604:Scorpion Venoms; D011188:Potassium