[Killing effect of VP3 on human nasopharyngeal carcinoma cell line CNE-2 cells].
OBJECTIVE
To investigate the killing effects of VP(3) on nasopharyngeal carcinoma cell line CNE-2.


METHODS
Plasmid expression vector pcDNA3.1(-) CMV.VP(3)-His was constructed and identified by Kpn I/EcoR I endonuclease analysis, and then sequenced to verify successful insertion in the sense direction of VP(3) gene. pcDNA3.1(-) CMV.VP(3)-His and pcDNA3.1(-)-His expression plasmid was transiently transfected into nasopharyngeal carcinoma cell line CNE-2 . VP(3) protein expression was detected by Western blotting. MTT assay was used to determine the killing effects of VP(3) gene on nasopharyngeal carcinoma cell line CNE-2.


RESULTS
Endonuclease analysis and sequencing confirmed the recombinant plasmid contained the complete VP(3) CDS sequence. Western blotting detected a 14.03 kD protein expression from the transfected cells, which was the expecting band of VP(3) gene. The growth of CNE-2 cells that expressed VP(3) gene was inhibited,while the growth of CNE-2 cells that did not express VP(3) gene was not inhibited.


CONCLUSIONS
VP(3) gene can kill nasopharyngeal carcinoma cell CNE-2.
Xu
Jing
J
Department of Otolaryngology, Xiangya Hospital, Central South University, Changsha 410008, China.
Qiu
Yuan-zheng
YZ
Tang
Yao-yun
YY
Tian
Yong-quan
YQ
Xiao
Xian-zhong
XZ
Zhao
Su-ping
SP
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D015316:Genetic Therapy; D006801:Humans; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D014162:Transfection
D000970:Antineoplastic Agents; D036022:Capsid Proteins; C091717:VP3 protein, Chicken anemia virus