Differential gene expression profiling of vocal fold polyps and Reinke's edema by complementary DNA microarray.
OBJECTIVE
Our purpose was to determine whether complementary DNA (cDNA) microarray analysis (MA) can establish distinct gene expression profiles for 2 phenotypically similar vocal fold lesions: Reinke's edema (RE) and polyps. Established transcript profiles can provide insight into the molecular and cellular processes involved in these diseases.


METHODS
Eleven RE specimens and 17 polyps were analyzed with MA for 8,745 genes. Further MA profiling was attempted within each lesion group to identify molecular markers for reflux exposure and smoking. Prediction analysis was used to predict lesion classification for 2 unclassified samples. A real-time polymerase chain reaction was performed to corroborate MA transcript levels for selected significant genes.


RESULTS
Sixty-five genes were found to differentiate RE and polyps (p = .0088). For RE, 19 genes were differentiated for reflux exposure (p = .016). No genes were found to differentiate smokers from nonsmokers. For polyps, no genes were found to differentiate for reflux (p = .16) and smoking (p = .565). Categorization of unclassified lesions was possible with a minimum of 13 genes.


CONCLUSIONS
We demonstrate the feasibility of benign lesion classification based on MA. Microarray analysis is useful not only for improving diagnosis and classification of such lesions, but also for potentially generating prognostic indicators and targets for therapy.
Duflo
Suzy M
SM
Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, School of Medicine, The University of Utah, Salt Lake City, Utah, USA.
Thibeault
Susan L
SL
Li
Wenhua
W
Smith
Marshall E
ME
Schade
Goetz
G
Hess
Markus M
MM
D000328:Adult; D018076:DNA, Complementary / Q000032:analysis*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007818:Laryngeal Diseases / Q000235:genetics*; D007819:Laryngeal Edema / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011127:Polyps / Q000235:genetics*; D014827:Vocal Cords
D018076:DNA, Complementary