[Immunohistochemical staining of the macrophagic and astrocytic response in the brainstem of Wistar rats submitted to the ethidium bromide gliotoxic model and treated with cyclophosphamide].
The gliotoxic ethidium bromide (EB) was used to study morphologically the macrophagic and astrocytic response under immunosuppression by cyclophosphamide (CY). Astrocyte immunoreactivity to glial fibrillary acidic protein (GFAP) and vimentin (VIM) and macrophagic immunoreactivity to ED1 were investigated after EB injection. Male Wistar rats were injected with 0.9% saline solution (group I), 0.1% BE (group II) and 0.1% EB associated with CY treatment (group III). Brainstem samples were collected from the 1st to the 21st day post-injection for GFAP, VIM and ED1 immunostaining. In groups II and III, it was observed increased immunoreactivity to GFAP and reexpression of VIM. In group II, ED1-positive cells were noted after the 2nd day and in group III, after the 3rd day. On the 14th day post-injection, it was observed a greater quantity of ED1- positive cells in group III than in group II. Apparently, CY did not change the astrocytic response pattern.
Sanchez
Melissa
M
Universidade Paulista, São Paulo, SP, Brazil. melissanchez@uol.com.br
Bondan
Eduardo Fernandes
EF
Lallo
Maria Anete
MA
Sinhorini
Idércio Luiz
IL
Dagli
Maria Lúcia Zaidan
ML
Maiorka
Paulo Cesar
PC
Pereira
Luis Antonio Violin Dias
LA
Graça
Dominguita Lühers
DL
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000473:pathology; D001933:Brain Stem / Q000187:drug effects / Q000473:pathology*; D003520:Cyclophosphamide / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004996:Ethidium / Q000633:toxicity*; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D008264:Macrophages / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling
D007166:Immunosuppressive Agents; D003520:Cyclophosphamide; D004996:Ethidium