A novel butyrolactone derivative inhibited apoptosis and depressed integrin beta4 expression in vascular endothelial cells.
To understand the effects of a novel butyrolactone derivative, 3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one (3BDO), on the apoptosis of vascular endothelial cells (VECs), we exposed 3BDO (20-60 microg/ml) to VECs deprived of serum and FGF-2 for 24 and 48 h, respectively. The results showed that 3BDO (20-60 microg/ml) increased VEC viability and inhibited VEC apoptosis induced by deprivation of serum and FGF-2 in a very weak dose-dependent manner. During this process, integrin beta4 expression was depressed, but the level of reactive oxygen species (ROS) was not changed. The data suggested that 3BDO (20-60 microg/ml) could inhibit VEC apoptosis and suppress integrin beta4 expression, but it could not depress the ROS level induced by deprivation of serum and FGF-2.
Wang
Weiwei
W
Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China.
Liu
Xia
X
Zhao
Jing
J
Zhao
Baoxiang
B
Zhang
Shangli
S
Miao
Junying
J
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004247:DNA / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D039663:Integrin beta4 / Q000096:biosynthesis*; D017382:Reactive Oxygen Species / Q000378:metabolism
D039663:Integrin beta4; D017382:Reactive Oxygen Species; D004247:DNA; D015107:4-Butyrolactone