Oxygen consumption of cell suspension in a poly(dimethylsiloxane) (PDMS) microchannel estimated by scanning electrochemical microscopy.
A quantitative analysis of the oxygen concentration profile near a poly(dimethylsiloxane) (PDMS) microfluidic device was performed using scanning electrochemical microscopy (SECM). A microchannel filled with sodium sulfite (Na(2)SO(3)) aqueous solution was imaged by SECM, showing that the oxygen diffusion layer of the PDMS microchannel was observed to be hemicylindrical. Based on a theoretical analysis of the hemicylindrical diffusion layer of the microchannel, the total oxygen mass transfer rates of oxygen to the PDMS microchannel filled with the Na(2)SO(3) solution was calculated to be (4.01 +/- 0.30) x 10(-12) mol s(-1). This is the maximum value of the oxygen transfer rate for this PDMS microchannel device. The oxygen consumption rate increased almost linearly with the logarithm of the concentration of E. coli cells (10(6) approximately 10(8) cells). The respiratory activity for a single E. coli cell was estimated to be approximately 4.31 x 10(-20) mol s(-1) cell(-1).
Saito
Takeshi
T
Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki, Aoba-ku, Sendai 980-8579, Japan.
Wu
Ching-Chou
CC
Shiku
Hitoshi
H
Yasukawa
Tomoyuki
T
Yokoo
Masaki
M
Ito-Sasaki
Takashi
T
Abe
Hiroyuki
H
Hoshi
Hiroyoshi
H
Matsue
Tomokazu
T
D002477:Cells / Q000378:metabolism*; D004058:Diffusion; D004129:Dimethylpolysiloxanes; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption
D004129:Dimethylpolysiloxanes; D010100:Oxygen