title: A rapid and efficient method for generating anti-variable region monoclonal antibodies using type-1 interferons as immune modulators.
abstract: The generation of anti-variable region monoclonal antibodies (mAbs) against therapeutic antibodies is essential in the pharmacokinetic/pharmacodynamic (PK/PD) assessments of the drugs in clinical study samples. Sandwich EIA and other methods are typically employed to achieve sensitivity and selectivity for the PK/PD analyses. These assays usually require generation of mAb reagents that bind specifically to the drug in non-competing pair combinations. Thus, large panels of anti-variable region mAbs must be generated in an expeditious manner to increase the probability of success. Herein we describe a novel immunization method that utilizes type 1 interferons (IFNs) as immunomodulators coupled with an agonistic anti-CD40 mAb as a B cell proliferative agent to drive immune responses. This novel protocol allows for rapid and robust mAb reagent generation without the use of conventional protein-denaturing adjuvants. The use of IFNs allowed for the generation of comparable and in some cases, increased numbers of anti-variable region mAbs in a dramatically shorter timeframe. This IFN based, immunostimulatory approach utilizing a non-denaturing adjuvant, likely presents conformational epitopes and may optimize the humoral response for the rapid generation of anti-variable region reagents to therapeutic mAb candidates.
authors.affiliation: Centocor Research & Development Inc., 145 King of Prussia Rd., Radnor, PA 19087, USA. KStaquet@cntus.jnj.com
authors.forename: Kimberly
authors.initials: K
authors.lastname: Staquet
authors.forename: Jamie
authors.initials: J
authors.lastname: Fisher
authors.forename: Roberta
authors.initials: R
authors.lastname: Lamb
authors.forename: Gregory
authors.initials: G
authors.lastname: Bannish
authors.forename: Cynthia
authors.initials: C
authors.lastname: Duchala
authors.forename: M Lamine
authors.initials: ML
authors.lastname: Mbow
authors.forename: Michael A
authors.initials: MA
authors.lastname: Rycyzyn
authors.forename: Jill
authors.initials: J
authors.lastname: Giles-Komar
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007372:Interferons / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D013154:Spleen / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D007372:Interferons