[Inhibitory effect of reinioside C on LOX-1 expression induced by ox-LDL].
OBJECTIVE
To investigate the effect of reinioside C (RC) on the expression of lectin-like oxidized low density lipoprotein receptor (LOX)-1 mRNA and LOX-1 protein induced by oxidized low density lipoprotein (ox-LDL) in cultured human umbilical vein endothelial cells (HUVEC).


METHODS
HUVECs were cultured with ox-LDL (50 mg/L) for 24 h in the absence or presence of RC (1, 3, and 10 micromol/L). The expressions of LOX-1 mRNA and LOX-1 protein were examined by RT-PCR and Western-blot.


RESULTS
Incubation with ox-LDL (50 mg/L) significantly raised the expression of LOX-1 mRNA and LOX-1 protein,which was concentration-dependent.


CONCLUSIONS
RC can inhibit the increased expression of LOX-1 mRNA and LOX-1 protein induced by ox-LDL in HUVECs.
Bai
Yong-ping
YP
Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhang
Guo-gang
GG
Shi
Rui-zheng
RZ
Li
Yuan-jian
YJ
Tan
Gui-shan
GS
Chen
Jia
J
D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D031801:Polygala / Q000737:chemistry*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011973:Receptors, LDL / Q000096:biosynthesis* / Q000235:genetics; D012503:Saponins / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; D011973:Receptors, LDL; D012503:Saponins; C099252:oxidized low density lipoprotein; C530274:reinioside C