Expression profile of Bag 1 in the postmortem brain.
Bag 1 is a protein intimately involved in signaling pathways that regulate cell survival. Here we examined the expression profile of Bag 1 in the brain to consider issues associated with the sampling of anti-apoptotic proteins in a rat model of the human postmortem process. Following a 4h postmortem interval, we analyzed the hippocampus of rats maintained at 24 or 4 degrees C storage temperatures using immunocytochemical and Western blotting techniques. Remarkably, postmortem tissue (up to 4h) showed a significant and prominent up-regulation of Bag 1 in CA1 and CA3 subfields of the hippocampal formation. Over-expression of Bag 1, however, could only be traced down to a storage temperature of 24 degrees C. These data suggest that storage temperatures, but not postmortem intervals, significantly affect the expression profile and cellular stability of Bag 1 proteins.
Curcio
Christine
C
Department of Neuroscience, New York College of Osteopathic Medicine of New York Institute of Technology, Old Westbury, NY 11568, USA.
Asheld
John J
JJ
Chabla
Janet M
JM
Ayubcha
Diana
D
Hallas
Brian H
BH
Horowitz
Judith M
JM
Torres
German
G
D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D016477:Artifacts; D001344:Autopsy / Q000592:standards; D001831:Body Temperature / Q000502:physiology; D001921:Brain / Q000378:metabolism* / Q000503:physiopathology; D001926:Brain Death / Q000378:metabolism / Q000503:physiopathology; D016923:Cell Death / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism* / Q000503:physiopathology; D007150:Immunohistochemistry; D008297:Male; D009410:Nerve Degeneration / Q000378:metabolism* / Q000503:physiopathology; D011180:Postmortem Changes; D051381:Rats; D020318:Rats, Long-Evans; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology
D051017:Apoptosis Regulatory Proteins; C091732:BCL2-associated athanogene 1 protein; D004268:DNA-Binding Proteins; D014157:Transcription Factors