[Evaluation of preparing process of extract of hypericum perforatum using HPLC fingerprint analysis].
OBJECTIVE
To establish the quality control method of the extract enriched hypericins and flavonoids (HFEE) prepared from Hypericum perforatum by means of HPLC fingerprint analysis, and to evaluate the validity, stability of within-day and between-day of the preparing process of HFEE as well as the influence of antioxidant and the herbal source to the quality of HFEE.


METHODS
HPLC-UV-MS was employed to the qualitative and quantitative analyses of HFEE.


RESULTS
Ten peaks on the HPLC fingerprint of HFEE were indicated, and eight compounds of them had been identified. With respect of the preparing process of HFEE, the validity and stability were significantly observed. There was almost no effect observed on the quality of HFEE whether or not adding the antioxidant during the process. However, different parts of the plant collected as the materials could significantly affect the quality of HFEE.


CONCLUSIONS
The method established in the present study is convenient, reliable, and could be used for the quality control of the extract of H. perforatum and for the monitor and control of the preparing process of the extract.
Wang
Dong-mei
DM
Laboratory of Pharmacognosy and Natural Medicinal Chemistry, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510080, China.
Liu
Chao-shen
CS
Yang
De-po
DP
D000873:Anthracenes; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D020902:Hypericum / Q000737:chemistry*; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D011794:Quercetin / Q000737:chemistry / Q000302:isolation & purification; D012431:Rutin / Q000737:chemistry / Q000302:isolation & purification; D013678:Technology, Pharmaceutical
D000873:Anthracenes; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012431:Rutin; D010569:Perylene; C004965:hypericin; D011794:Quercetin