Bioadhesive properties of poly(alkylcyanoacrylate) nanoparticles coated with polysaccharide.
Development of bioadhesive nanoparticles is of great interest to improve drug absorption through the intestinal barrier. Various Polysaccharide-coated poly(alkylcyanoacrylate) nanoparticles were prepared. The bioadhesive properties of the nanoparticles coated with dextran or chitosan in end-on or side-on conformation were evaluated with an ex-vivo adsorption experiment on rat intestine. Results show that diffusion of nanoparticles in mucus layer was governed by the nanoparticle diameter and isotherms of adsorption were influenced by the nature of polysaccharide used. High amount of nanoparticles coated with chitosan can be entrapped in the mucus layer even at low nanoparticle concentration in suspension. When nanoparticle concentration increased, a pseudo-plateau was reached. In the case of dextran-coated nanoparticles, linear increase of adsorption was observed and no saturation phenomenon was highlighted over the range of nanoparticle concentration used in this study. These results suggested that interactions involved in bioadhesion mechanism depended on the nature of polysaccharide. Electrostatic interactions are enhanced between chitosan-coated nanoparticles and glycoproteins of mucus leading to a saturated adsorption phenomenon whereas dextran-coated nanoparticles interacted by non-electrostatic interactions with mucus resulting in a non-saturated phenomenon. Polysaccharides grafted at the nanoparticle surface in the brush conformation appeared more favorable to promote interactions of nanoparticles with glycoproteins of mucus in comparison with the more compact loop conformation of polysaccharide chains.
Bertholon
Isabelle
I
Laboratoire de Physico-chimie, Biopharmacie, Pharmacotechnie, UMR CNRS 8612, Faculté de Pharmacie, 5 rue Jean-Baptiste Clément, 92296 Châtenay-Malabry, France.
Ponchel
Gilles
G
Labarre
Denis
D
Couvreur
Patrick
P
Vauthier
Christine
C
D000268:Adhesiveness; D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003487:Cyanoacrylates / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000737:chemistry*; D007421:Intestine, Small / Q000737:chemistry; D008297:Male; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011134:Polysaccharides / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D014014:Tissue Adhesives / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D003487:Cyanoacrylates; D004337:Drug Carriers; D011134:Polysaccharides; D014014:Tissue Adhesives