title: Resveratrol inhibits firefly luciferase.
abstract: The potential therapeutic value of resveratrol in age-related disease settings including cancer, diabetes, and Alzheimer's has emerged from a rapidly growing body of experimental evidence. Protection from oxidative stress appears to be a common feature of resveratrol that may be mediated through SirT1, though more specific molecular mechanisms by which resveratrol mediates its effects remain unclear. This has prompted an upsurge in cell-based mechanistic studies, often incorporating reporter assays for pathway elucidation in response to resveratrol treatment. Here, we report that resveratrol potently inhibits firefly luciferase with a K(i) value of 2microM, and caution that this confounding element may lead to compromised data interpretation.
authors.affiliation: Diabetes and Metabolism Disease Area, Novartis Institutes for BioMedical Research, 100 Technology Square, Cambridge, MA 02139, USA.
authors.forename: Adel
authors.initials: A
authors.lastname: Bakhtiarova
authors.forename: Paul
authors.initials: P
authors.lastname: Taslimi
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Elliman
authors.forename: Penelope A
authors.initials: PA
authors.lastname: Kosinski
authors.forename: Brian
authors.initials: B
authors.lastname: Hubbard
authors.forename: Michael
authors.initials: M
authors.lastname: Kavana
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Kemp
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D017930:Genes, Reporter; D049409:Luciferases, Firefly / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D049409:Luciferases, Firefly; D000077185:Resveratrol