title: Preparation of monoclonal antibody bank against whole water-soluble proteins from rapid-growing bamboo shoots.
abstract: An antibody bank against the whole proteins in a proteome is a useful tool for biological research. Using the standard cell fusion method, and a modified screening protocol, we produced an mAb bank against the total water-soluble proteins extracted from the rapid-growing green bamboo shoots. An improved two-stage strategy was employed to enrich those poor immunogenic or lower expressed proteins. Totally, we obtained a bank of 192 mAb which were identified as distinctive to each other by 2-DE and immunostaining.
authors.affiliation: Department of Biochemical Science and Technology, Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Yu-Jen
authors.initials: YJ
authors.lastname: Wu
authors.forename: Han-Min
authors.initials: HM
authors.lastname: Chen
authors.forename: Tai-Tse
authors.initials: TT
authors.lastname: Wu
authors.forename: Jiann-Shing
authors.initials: JS
authors.lastname: Wu
authors.forename: Rea-Min
authors.initials: RM
authors.lastname: Chu
authors.forename: Rong-Huay
authors.initials: RH
authors.lastname: Juang
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D031723:Bambusa / Q000276:immunology*; D004353:Drug Evaluation, Preclinical; D007123:Immunoelectrophoresis, Two-Dimensional; D011506:Proteins / Q000276:immunology*; D020543:Proteome / Q000276:immunology / Q000378:metabolism*; D012995:Solubility; D014867:Water
chemical_list: D000911:Antibodies, Monoclonal; D011506:Proteins; D020543:Proteome; D014867:Water