title: Induction of transcription factor A-myb expression in reactive astrocytes following an excitotoxic lesion in the mouse hippocampus.
abstract: In the present study, we examined patterns of A-myb expression in the kainic acid (KA)-treated mouse hippocampus. Western blot analysis revealed that A-myb expression was dramatically increased in brain 3 days after KA treatment, and was sustained for more than 7 days. A-myb immunoreactivity was restricted to hippocampal neurons in control mice. Three days after KA treatment, strong A-myb immunoreactivity was observed in reactive astrocytes throughout the CA3 region. Thereafter, A-myb immunoreactive astrocytes gradually concentrated around the CA3 region in parallel with selective neuronal loss, and only a few A-myb immunoreactive astrocytes persisted in the CA3 region 14 days after KA treatment. These findings suggest that the A-myb plays a role in the reactive gliosis signaling pathway in KA-induced excitotoxic lesions.
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine, Yongon-Dong, Chongno-Gu, Seoul 110-799, Korea.
authors.forename: Gye Sun
authors.initials: GS
authors.lastname: Jeon
authors.forename: Hee Jine
authors.initials: HJ
authors.lastname: Byun
authors.forename: Sung Kyung
authors.initials: SK
authors.lastname: Park
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Park
authors.forename: Dong Woon
authors.initials: DW
authors.lastname: Kim
authors.forename: Je Hoon
authors.initials: JH
authors.lastname: Seo
authors.forename: Choong Ik
authors.initials: CI
authors.lastname: Cha
authors.forename: Sa Sun
authors.initials: SS
authors.lastname: Cho
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D015153:Blotting, Western; D003720:Densitometry; D018690:Excitatory Amino Acid Agonists / Q000008:administration & dosage / Q000633:toxicity*; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000473:pathology*; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D007608:Kainic Acid / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D020598:Proto-Oncogene Proteins c-myb; D015398:Signal Transduction / Q000187:drug effects; D015534:Trans-Activators / Q000096:biosynthesis*
chemical_list: D018690:Excitatory Amino Acid Agonists; C490283:Mybl1 protein, mouse; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D015534:Trans-Activators; D007608:Kainic Acid