[Antivirus effects of extract from gardenia].
OBJECTIVE
To observe the effect of the extract from gardenia on influenza viral pneumonia in mice and virus-induced cytopathic effect.


METHODS
The mice were infected by influenza virus in nasal, the lung inflammation, mortality rate and life elongation rate were observed respectively. The anti-viral activity of the extract from gardenia was accessed by cytopathic effect (CPE) in vitro and 0% toxicity concentration (TC0), 50% toxicity concentration (TC50), 50% inhibitor concentration (IC50), therapeutic index (TI) were determined by Reed-Muench method.


RESULTS
The pneumonia induced by influenza virus in mice was inhibited significantly by the extract from gardenia, as the mortality rate decreased and the life elongation rate increased remarkably. Meanwhile the NO content in serum decreased significantly; The cytopathic effect induced by six kinds of viruses was inhibited remarkably.


CONCLUSIONS
The six kinds of viruses were inhibited significantly by the extract from gardenia which inhibitory effect on mice influenza viral pneumonia was related to the NO content decreased.
Wang
Yi-Zhong
YZ
Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Belting 100700, China.
Cui
Xiao-Lan
XL
Gao
Ying-Jie
YJ
Guo
Shan-Shan
SS
Wang
Xiu-Kun
XK
Huang
Yang
Y
Zhao
Ye
Y
Gong
Weng-Feng
WF
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000821:virology; D004947:Esophagus / Q000166:cytology / Q000821:virology; D005260:Female; D032062:Gardenia / Q000737:chemistry; D018259:Herpesvirus 1, Human / Q000187:drug effects; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000097:blood; D009975:Orthomyxoviridae / Q000472:pathogenicity; D010946:Plants, Medicinal / Q000737:chemistry; D011024:Pneumonia, Viral / Q000097:blood / Q000188:drug therapy*; D011897:Random Allocation; D018113:Respiratory Syncytial Virus, Human / Q000187:drug effects
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D009569:Nitric Oxide