title: Protection of normal human reconstructed epidermis from UV by catalase overexpression.
abstract: Reactive oxygen species (ROS) generated by ultraviolet (UV) irradiation are counterbalanced by endogenous antioxidant systems. To test the hypothesis of a novel photoprotective approach, we irradiated epidermis reconstructed with normal human keratinocytes overexpressing sustainably lentivirus-mediated catalase (CAT), copper/zinc superoxide dismutase (CuZnSOD) or manganese superoxide dismutase (MnSOD) enzymes. We found that following UVB irradiation there was a marked decrease in sunburn cell formation, caspase-3 activation and p53 accumulation in human reconstructed epidermis overexpressing CAT. Moreover, UVA-induced hypertrophy and DNA oxidation (8-oxodeoxyguanosine) were decreased by CAT overexpression. These effects were not achieved by overexpression of CuZnSOD or MnSOD. In conclusion, vector-mediated CAT overexpression could be a promising photoprotective tool against deleterious effects of UV irradiation such skin cancer especially in monogenic/polygenic photosensitive disorders characterized by ROS accumulation.
authors.affiliation: INSERM E 0217, University Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, France.
authors.forename: H R
authors.initials: HR
authors.lastname: Rezvani
authors.forename: M
authors.initials: M
authors.lastname: Cario-André
authors.forename: C
authors.initials: C
authors.lastname: Pain
authors.forename: C
authors.initials: C
authors.lastname: Ged
authors.forename: H
authors.initials: H
authors.lastname: deVerneuil
authors.forename: A
authors.initials: A
authors.lastname: Taïeb
mesh_terms: D017209:Apoptosis; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D004817:Epidermis / Q000201:enzymology / Q000528:radiation effects*; D006801:Humans; D007150:Immunohistochemistry; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic; D019076:Transgenes; D014466:Ultraviolet Rays
chemical_list: D002374:Catalase; D013482:Superoxide Dismutase