Comparative study of the water oxidizing reactions and the millisecond delayed chlorophyll fluorescence in photosystem II at different pH.
Water splitting activity, the multiline EPR signal associated with S(2)-state of the CaMn(4)-cluster and the fast and slow phases of the induction curve of the millisecond delayed chlorophyll fluorescence from photosystem II (PSII) in the pH range of 4.5-8.5 were studied in the thylakoid membranes and purified PSII particles. It has been found that O(2) evolution and the multiline EPR signal were inhibited at acidic (pK approximately 5.3) and alkaline (pK approximately 8.1) pH values, and were maximal at pH 6.0-7.0. Our results indicate that the loss of O(2) evolution and the S(2)-state multiline EPR signal associated with the decrease of the millisecond delayed chlorophyll fluorescence only in alkaline region (pH 7.0-8.5). Possible correlations of the millisecond delayed chlorophyll fluorescence components with the donor side reactions in PSII are discussed.
Gasanov
Ralphreed
R
Biophysics Laboratory, Institute of Botany, National Academy of Sciences, Patamdar Road 40, Baku AZ-1073, Azerbaijan. r.gasanov@box.az
Aliyeva
Samira
S
Arao
Sachiko
S
Ismailova
Aygun
A
Katsuta
Nobuhiro
N
Kitade
Hidetoshi
H
Yamada
Shuji
S
Kawamori
Asako
A
Mamedov
Fikret
F
D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D014867:Water
D045332:Photosystem II Protein Complex; D014867:Water; D002734:Chlorophyll; D010100:Oxygen