title: Transplantation of human CD34+ stem cells from umbilical cord blood to rats with thioacetamide-induced liver cirrhosis.
abstract: BACKGROUND
  Liver fibrosis results from accumulation of extracellular matrix components and is associated with many chronic hepatic diseases. There is to date no specific therapy for this disease, and patients receive treatment for its associated complications. Specific progenitor cells, known as oval cells, are present in the liver. As oval cells express markers such as CD34, they are thought to arise from a hematopoietic precursor. The aim of this work was to investigate whether transplantation of hematopoietic CD34(+) stem cells could improve hepatic fibrosis by their differentiation into hepatocytes.
  
  
  METHODS
  CD34(+) stem cells from human umbilical cord blood were purified, transduced with a lentiviral vector containing the green fluorescent protein (GFP) gene and injected via portal vein into rats with liver cirrhosis induced by the 4-month administration of thioacetamide. Rats were killed 15 and 60 days post-transplantation.
  
  
  RESULTS
  Up to 37% and 22% fluorescent cells were observed in the blood of control and cirrhotic rats, respectively, at 15 days post-transplantation. At 60 days post-transplantation, however, fluorescent cells were completely absent from the blood. Fluorescence was not detected in liver sections at either 15 or 60 days post-transplantation. Polymerase chain-reaction study to detect the GFP gene ruled out silencing of the transgene.
  
  
  CONCLUSIONS
  These results suggest that the transplanted cells did not engraft in the liver and were eliminated from the rats.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Granada, Granada, Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: Sáez-Lara
authors.forename: Cecilia
authors.initials: C
authors.lastname: Frecha
authors.forename: Francisco
authors.initials: F
authors.lastname: Martín
authors.forename: Francisco
authors.initials: F
authors.lastname: Abadía
authors.forename: Miguel
authors.initials: M
authors.lastname: Toscano
authors.forename: Angel
authors.initials: A
authors.lastname: Gil
authors.forename: Luis
authors.initials: L
authors.lastname: Fontana
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology*; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D036101:Cord Blood Stem Cell Transplantation; D006801:Humans; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000276:immunology* / Q000601:surgery*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D033581:Stem Cell Transplantation; D013853:Thioacetamide / Q000494:pharmacology*; D014183:Transplantation, Heterologous / Q000276:immunology
chemical_list: D018952:Antigens, CD34; D013853:Thioacetamide