[Experimental study on acting mechanism of vicera purging method in purging fu-organs and benefiting fei].
OBJECTIVE
To explore the acting mechanism of viscera purging method (VP) in purging Fu-organs and benefiting Fei from integral, cellular and molecular levels.


METHODS
Forty SD rats were equally divided into four groups randomly: the normal group, the model group, the unhitch group and the VP group. Except those in the normal group were untreated, rats were established to intestinal obstruction model by incomplete ligation of the rectum in vitro. The ligation was relieved 48 h after operation in the unhitch group and the VP group, and the animals were fed continuously on routine. Meanwhile, Dachengqi Decoction (DD) 2 ml was given twice a day to the VP group for 2 days. Finally, the serum interleukin 8 (IL-8) and mRNA expression of tumor necrosis factor-alpha (TNF-alpha) in the lung tissue were detected by radioimmunoassay and RT-PCR respectively. Besides, the number of pulmonary alveolar macrophages (PAM) in the bronchial alveolus lavage fluid (BALF) was counted and their death rate calculated.


RESULTS
Compared with the normal control, the serum IL-8 content in lung tissue in the model rats were remarkably higher (P < 0.01); however, the VP group showed the lowest level of IL-8 content and the highest was shown in the model group. Number of PAM in BALF was higher and its death rate was lower in the VP group than that in the unhitch groups (both P< 0.05). The expression of TNF-alpha mRNA was sinificantly higher as compared with that in the normal group, and lowered after administration of DD.


CONCLUSIONS
Viscera purging method could protect the injured lung tissue to some extent.
Yang
Sheng-lan
SL
Xiehe Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan. yangshenglan@126.com
Chen
Rui
R
Li
Dao-ben
DB
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D016209:Interleukin-8 / Q000097:blood; D007415:Intestinal Obstruction / Q000150:complications / Q000188:drug therapy*; D008168:Lung / Q000378:metabolism; D008297:Male; D008516:Medicine, Chinese Traditional; D008517:Phytotherapy; D010936:Plant Extracts; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000209:etiology / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
D004365:Drugs, Chinese Herbal; D016209:Interleukin-8; D010936:Plant Extracts; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C477169:dachengqi decoction