title: Olfactometer and large cage evaluation of a solid phase technology for the controlled production of CO2.
abstract: New technology by ICA for chemical generation of carbon dioxide (CO2) was evaluated in olfactometer and large outdoor cage tests against laboratory-reared Aedes aegypti for potential use in mosquito surveillance programs. The proprietary CO2 generation system consists of a poly-Tyvek sachet containing 2 solid ingredients. Activated sachets immediately react to generate and release predictable levels of CO2 over time. In noncompetitive olfactometer studies, a freshly activated sachet attracted an average +/- SE of 96.6 +/- 0.9% of the available mosquitoes compared with 20.2 +/- 6.5% for 5 ml/min CO2 released from a compressed gas cylinder. In competitive tests, the sachet attracted 92.4 +/- 1.2% compared with 0.9 +/- 0.5% for the compressed gas. In the olfactometer, aged sachets attracted >90% of the available mosquitoes up to 8 h and ca. 27% 1 wk after activation. In the large outdoor cages, traps baited with activated sachets captured 2.2-5.4 times as many mosquitoes as unbaited traps, depending on time of testing after activation.
authors.affiliation: USDA/ARS, Center for Medical, Agricultural, and Veterinary Entomology, Gainesville, FL 32608, USA.
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Kline
authors.forename: Sandra A
authors.initials: SA
authors.lastname: Allan
authors.forename: Ulrich R
authors.initials: UR
authors.lastname: Bernier
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Posey
mesh_terms: D000818:Animals; D002245:Carbon Dioxide; D009033:Culicidae; D011159:Population Surveillance / Q000379:methods
chemical_list: D002245:Carbon Dioxide