title: [Determination of danshensu and protocatechuic aldehyde in Guanxinning injection powder by RP-HPLC].
abstract: OBJECTIVE
  An RP-HPLC procedure was established for the determination of danshensu and protocatechuic aldehyde in Guanxinning injection powder.
  
  
  METHODS
  An RP-HPLC analytical procedure was developed using Hypersil ODS2 C18 column (4.6 mm x 250 mm, 5 microm) with methanol-0.5% glaceal acetic acid (4.5:95.5) as mobile phase, and a wavelength of 280 nm for UV detection.
  
  
  RESULTS
  The linear range was 3.004-45.06 microg x m(L-1) (r = 0.999 5, n = 6) for danshensu, and 0.300-4.509 microg x mL(-1) (r = 0.999 3, n = 6) for protocatechuic aldehyde. The average recoveries were 99.1% and 97.9%, respectively.
  
  
  CONCLUSIONS
  The method was stable, accurate, and reproducible, and can be used for the quality control of Guanxinning injection powder.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Xiao-Rui
authors.initials: XR
authors.lastname: Guo
authors.forename: Wen-Ting
authors.initials: WT
authors.lastname: Zhang
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Kai-Shun
authors.initials: KS
authors.lastname: Bi
mesh_terms: D001547:Benzaldehydes / Q000032:analysis*; D002396:Catechols / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D007267:Injections; D007773:Lactates / Q000032:analysis*; D030004:Ligusticum / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011208:Powders; D011786:Quality Control; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry
chemical_list: D001547:Benzaldehydes; D002396:Catechols; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007773:Lactates; D011208:Powders; C035055:3,4-dihydroxyphenyllactic acid; C005581:protocatechualdehyde