title: [The anti-respiratory syncytial virus effect of active compound of Glycyrrhiza GD4 in vitro].
abstract: OBJECTIVE
  To study the effect on anti-respiratory syncytial virus of an active compound GD4 from Glycyrrhiza in vitro.
  
  
  METHODS
  GD4 was extracted from Glycyrrhiza by hot water extraction, dichloromethane extraction and column chromatography. The CPE inhibition assay was used to test the antiviral activity of GD4 on RSV in Hela cells.
  
  
  RESULTS
  GD4 was effective antiviral agent for RSV in a concentration-dependent manner and its median toxic concentration (TC50) and median effeicacious concentration (EC50) and treatment index (TI) was 0.23 mg/ml, 28.73 microg/ml and 8.0. 120 microg/ml GD4 exhibited inhibitory effect when added into cell culture for every 2h within 12h post-infection. GD4 didn't contain glycyrrhizic acid.
  
  
  CONCLUSIONS
  The active compound of Glycyrrhiza (GD4) was an evident inhibitory effect on RSV.
authors.affiliation: Public Health College of Harbin Medical University, China.
authors.forename: Xiu-qin
authors.initials: XQ
authors.lastname: Wang
authors.forename: Hong-yuan
authors.initials: HY
authors.lastname: Li
authors.forename: Xin-yan
authors.initials: XY
authors.lastname: Liu
authors.forename: Feng-mei
authors.initials: FM
authors.lastname: Zhang
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Ying-ai
authors.initials: YA
authors.lastname: Piao
authors.forename: Zhi-ping
authors.initials: ZP
authors.lastname: Xie
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Li
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D006035:Glycyrrhiza / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D010946:Plants, Medicinal / Q000737:chemistry*; D012136:Respiratory Syncytial Viruses / Q000187:drug effects*; D012254:Ribavirin / Q000008:administration & dosage / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D012254:Ribavirin