Genetic architecture of Hsp90-dependent drug resistance.
Hsp90 potentiates the evolution of azole resistance in the model yeast Saccharomyces cerevisiae and the opportunistic pathogen Candida albicans via calcineurin. Here, we explored effectors downstream of calcineurin regulating this Hsp90-dependent trait. Using S. cerevisiae erg3 mutants as a model, we determined that both Crz1 and Hph1 modulate azole resistance.
Cowen
Leah E
LE
Whitehead Institute, Nine Cambridge Center, Cambridge, MA 02142, USA.
Carpenter
Anne E
AE
Matangkasombut
Oranart
O
Fink
Gerald R
GR
Lindquist
Susan
S
D000935:Antifungal Agents / Q000494:pharmacology; D019703:Calcineurin / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000235:genetics; D015725:Fluconazole / Q000494:pharmacology; D005800:Genes, Fungal; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*
D000935:Antifungal Agents; C074510:HSP82 protein, S cerevisiae; D018841:HSP90 Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; D015725:Fluconazole; D010088:Oxidoreductases; C039306:sterol delta-5 desaturase; D019703:Calcineurin