Dual-labeled telomere sensing probes for quantification of telomerase activity assay.
The present study describes an empirically discovered phenomenon that might be useful for development of a sensitive and rapid methodology for quantification of telomerase activity assay with simple data acquisition and possibility for calculation of telomerase product in absolute units. The method is based on the design and application of two single-stranded telomere sensing probes consisting of dual-labeled 16-mer oligonucleotides (fluorescent Cy3/Cy3-labeled and non-fluorescent IowaBlack/BHQ-labeled) that can simultaneously hybridize on the primary product of the telomerase reaction.
Bakalova
Rumiana
R
On-site Sensing and Diagnosis Research Laboratory, AIST-Kyushu, 807-1 Shuku-machi, Tosu 841-0052, Japan. r.bakalova-zheleva@aist.go.jp
Zhelev
Zhivko
Z
Kubo
Takanori
T
Mileva
Milka
M
Ohba
Hideki
H
D001483:Base Sequence; D002232:Carbocyanines; D015342:DNA Probes / Q000235:genetics; D005456:Fluorescent Dyes; D015336:Molecular Probe Techniques; D019098:Telomerase / Q000032:analysis*; D016615:Telomere / Q000201:enzymology* / Q000235:genetics
D002232:Carbocyanines; D015342:DNA Probes; D005456:Fluorescent Dyes; C089187:cyanine dye 3; D019098:Telomerase