title: Characterization of circulating blood dendritic cell subsets DC123+ (lymphoid) and DC11C+ (myeloid) in prostate adenocarcinoma patients.
abstract: OBJECTIVE
  We verified whether prostate adenocarcinoma produces specific modifications in DC subsets count.
  
  
  METHODS
  Twenty-one untreated prostate adenocarcinomas were divided on the basis of clinical stage in localized and metastatic disease. As control we used a population of 18 healthy male subjects. For DCs enumeration, peripheral blood (PB) samples were obtained in all cases. A single-platform flow cytometric assay based on Tru-COUNT was used for the enumeration of the two DCs subsets, myeloid (mDCs) and plasmacytoid (pDCs).
  
  
  RESULTS
  We showed a statistically significant reduction in pDCs count in prostate cancer population when compared to healthy controls (P = 0.002). Comparing each clinical stage with healthy controls, significant differences were found between controls and the metastatic group in both pDCs and mDCs (P = 0.005 and P = 0.023 respectively) but not between controls and the localized group (P = 0.055 and P = 0.829 respectively).
  
  
  CONCLUSIONS
  We showed that DCs count in PB is significantly affected by prostate adenocarcinoma progression in a metastatic disease.
authors.affiliation: Department of Urology, University of La Sapienza, Rome, Italy. sciarrajr@hotmail.com
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sciarra
authors.forename: Miriam
authors.initials: M
authors.lastname: Lichtner
authors.forename: Gomez AnnaMaria
authors.initials: GA
authors.lastname: Autran
authors.forename: Claudio
authors.initials: C
authors.lastname: Mastroianni
authors.forename: Raffaella
authors.initials: R
authors.lastname: Rossi
authors.forename: Fabio
authors.initials: F
authors.lastname: Mengoni
authors.forename: Cristiano
authors.initials: C
authors.lastname: Cristini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Gentilucci
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Vullo
authors.forename: Franco
authors.initials: F
authors.lastname: Di Silverio
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000473:pathology* / Q000556:secondary; D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count; D003713:Dendritic Cells / Q000145:classification / Q000378:metabolism / Q000473:pathology*; D018450:Disease Progression; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000097:blood / Q000473:pathology*