title: Glycerophospholipid molecular species in platelets and brain tissues - are platelets a good model for neurons?
abstract: The molecular classes of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) from the basal ganglia, cerebellum, cortex, erythrocytes and blood platelets of female rats were separated by an isocratic HPLC method using a silica column and ultraviolet detection. Each glycerophospholipid class were thereafter derivatized to dimethylphosphatidic acid (PA) molecular species, separated by reverse phase HPLC and detected by an evaporative laser scatter to quantify the different glycerophospholipid species. The distribution of molecular species in each class of the glycerophospholipids in the three brain areas was very similar with a predominance of the 18:0/22:6 species and very little of the 18:0/20:4 species. In contrast, the 18:0/20:4 species predominated in the blood cells which had a very low proportion of 18:0/22:6. These results are discussed on the background that platelets have been extensively used as a model for neurons and our previous physicochemical observation that phenothiazines appear to interact specifically with the 18:0/22:6 species of PS.
authors.affiliation: Laboratory of Clinical Biochemistry and The Blood Bank, Haukeland University Hospital, 5021 Bergen, Norway. anne.bakken@helse-bergen.no
authors.forename: Anne M
authors.initials: AM
authors.lastname: Bakken
authors.forename: Alexander
authors.initials: A
authors.lastname: Staeffler
authors.forename: Hugo A
authors.initials: HA
authors.lastname: Jørgensen
authors.forename: Holm
authors.initials: H
authors.lastname: Holmsen
mesh_terms: D000818:Animals; D001479:Basal Ganglia / Q000737:chemistry; D001792:Blood Platelets / Q000737:chemistry*; D001921:Brain / Q000166:cytology*; D002531:Cerebellum / Q000737:chemistry; D002540:Cerebral Cortex / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005260:Female; D020404:Glycerophospholipids / Q000032:analysis* / Q000145:classification; D008954:Models, Biological; D009474:Neurons / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010718:Phosphatidylserines / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020404:Glycerophospholipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; C483858:phosphatidylethanolamine