[Histological observations of chemically induced acute hepatic injury repaired by allogeneic bone marrow stem cell transplantation].
OBJECTIVE
To explore the possibility of repairing chemically induced acute hepatic injuries with allogeneic bone marrow stem cell (BMSC) transplantation.


METHODS
A SD rat model of CCl(4)-induced acute hepatic injury was established, which received transplantation of BMSCs (2.0 ml, 1x10(6)/ml) or normal saline injection into the local liver parenchyma, respectively. The rats were sacrificed at 6 h before and 6 h, 1, and 5 weeks after transplantation, and the livers were prepared for microscopic examination.


RESULTS
Cellular necrosis, bridging necrosis, congestion in the hepatic sinusoid, and inflammatory cell infiltration were seen in the chemically injured livers 6 h after model establishment, and these changes were ameliorated in rats receiving BMSC transplantation.


CONCLUSIONS
Allogeneic BMSC transplantation can repair chemically induced acute liver injuries.
Liu
Sheng-jun
SJ
Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Fang
Chi-hua
CH
Chen
Xiao-wu
XW
Rong
Zhen-xiang
ZX
Zhu
Da-jian
DJ
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000473:pathology / Q000601:surgery*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
D002251:Carbon Tetrachloride