Molecular mechanism of flucytosine resistance in Candida lusitaniae: contribution of the FCY2, FCY1, and FUR1 genes to 5-fluorouracil and fluconazole cross-resistance.
Inactivation of the FCY2 (cytosine permease), FCY1 (cytosine deaminase), and FUR1 (uracil phosphoribosyltransferase) genes in Candida lusitaniae produced two patterns of resistance to flucytosine. Mutant fur1 demonstrated resistance to 5-fluorouracil, whereas mutants fcy1 and fcy2 demonstrated fluconazole resistance in the presence of subinhibitory flucytosine concentrations.
Papon
Nicolas
N
Laboratoire des Sciences Végétales, EA209, UFR des Sciences Pharmaceutiques et Biologiques, Université Paris 5, 4 avenue de l'Observatoire, 75006 Paris, France.
Noël
Thierry
T
Florent
Martine
M
Gibot-Leclerc
Stéphanie
S
Jean
Dorothée
D
Chastin
Christiane
C
Villard
Jean
J
Chapeland-Leclerc
Florence
F
D000483:Alleles; D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D003001:Cloning, Molecular; D043525:Cytosine Deaminase / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D025141:Drug Resistance, Fungal / Q000235:genetics*; D015725:Fluconazole / Q000494:pharmacology*; D005437:Flucytosine / Q000494:pharmacology*; D005472:Fluorouracil / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics; D005816:Genetic Complementation Test; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D010430:Pentosyltransferases / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA
D000935:Antifungal Agents; D004271:DNA, Fungal; D005656:Fungal Proteins; D015725:Fluconazole; D005437:Flucytosine; D010430:Pentosyltransferases; C023269:uracil phosphoribosyltransferase; D043525:Cytosine Deaminase; D005472:Fluorouracil