Analysis of intracellular nucleotides by capillary electrophoresis-mass spectrometry.
A pilot study using capillary electrophoresis with mass spectrometry for the analysis of nucleotides in human erythrocytes is presented. Erythrocytes were incubated with 5-amino-4-imidazolecarboxamide riboside in order to mimic situation in defect of purine metabolism--AICA-ribosiduria. Characteristic AICA-ribotides together with normal nucleotides were separated by capillary electrophoresis in acetate buffer (20 mmol/L, pH 4.4) and identified on line by mass spectrometry.
Friedecký
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Laboratory for Inherited Metabolic Disorders, Department of Clinical Biochemistry, University Hospital Olomouc, Olomouc, Czech Republic.
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Adam
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D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods*; D009711:Nucleotides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D011687:Purines / Q000737:chemistry; D012263:Ribonucleosides / Q000737:chemistry / Q000494:pharmacology
D009711:Nucleotides; D011687:Purines; D012263:Ribonucleosides; D000620:Aminoimidazole Carboxamide; C011651:acadesine