[Study on establishment of system with high frequency for genetic transformation of Phellodendron chinese and plant regeneration].
OBJECTIVE
To establish an effective system for genetic transformation of Phellodendron chinese by Agrobacterium rhizogenes.


METHODS
A. rhizogenes strains R1601 was employed to induce the optimum hairy roots and the hairy roots were developed into regenerated plants on solid MS media with different kinds of plant growth regulators.


RESULTS
An effective protocol in optimized conditions were the hypocotyl with cotyledon after 10 d cultured, by 36 h for pre-culture, 15 min for infection, 4 d for co-culture and dosage 60 micromol/L AS in culture A. rhizogenes. It was clearly demonstrated that T-DNA of A. rhizogenes Ri plasmid was integrated into the cells of hairy roots and regenerated plants by the experiments of PCR.


CONCLUSIONS
A. rhizogenes strains R1601 can induce hairy roots of Phellodendron chinese in optimized conditions and the obtained hairy roots can develop into regenerated plants.
Wang
Yue-hua
YH
Department of Biological Engineering, Chengdu University, China.
D000098:Acetophenones / Q000494:pharmacology; D003470:Culture Media; D032102:Phellodendron / Q000235:genetics* / Q000254:growth & development; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012038:Regeneration; D012231:Rhizobium / Q000235:genetics*; D014170:Transformation, Genetic
D000098:Acetophenones; D003470:Culture Media