Cytokine gene expression patterns associated with immunization against Marek's disease in chickens.
The present study explored the immunological correlates of protection mediated by a live bivalent vaccine consisting of herpesvirus of turkeys (HVT) and SB-1 against infection with the RB1B strain of Marek's disease virus (MDV). Compared to unvaccinated infected chickens, vaccinated protected birds had lower expression of interleukin (IL)-6, IL-10 and IL-18 genes in spleen. However, there was no difference between these two groups of birds in the expression of interferon (IFN)-gamma, IL-4, IL-12 and inducible nitric oxide synthase (iNOS) genes on day 21 post-infection. Furthermore, protection was associated with lower MDV genome load in spleen but not in feather tips, suggesting that vaccination had little or no effect on curtailing virus transmission. In conclusion, vaccination with a bivalent MD vaccine was associated with distinct cytokine expression patterns in spleen and modulation of cytokine responses by the vaccine may play a role in mediation of protection.
Abdul-Careem
Mohamed Faizal
MF
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ont. N1G 2W1, Canada.
Hunter
Bruce D
BD
Parvizi
Payvand
P
Haghighi
Hamid R
HR
Thanthrige-Don
Niroshan
N
Sharif
Shayan
S
D000818:Animals; D002645:Chickens / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D017931:DNA Primers; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics; D003627:Data Interpretation, Statistical; D005241:Feathers / Q000276:immunology; D005786:Gene Expression Regulation; D008381:Herpesvirus 2, Gallid / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology / Q000378:metabolism / Q000821:virology; D008380:Marek Disease / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D022501:Marek Disease Vaccines / Q000276:immunology*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000276:immunology / Q000378:metabolism / Q000821:virology
D016207:Cytokines; D017931:DNA Primers; D004279:DNA, Viral; D022501:Marek Disease Vaccines; D012367:RNA, Viral; D052247:Nitric Oxide Synthase Type II