title: [Determination of ligustilide for quality assessment of Ligusticum chuanxiong].
abstract: OBJECTIVE
  To assay ligustilide content in the herb of Szechwan Lovage Rhizome (Chuanxiong, CX), which is the dried rhizome of Ligusticum chuanxiong in order to assess the quality.
  
  
  METHODS
  Ligustilide was quantitatively analyzed by high-performance liquid chromatography in 21 CX samples. An Alltima C18 column (4.6 mmx 150 mm, 5 microm) was used as the analytical column. The mobile phase consisted of water and acetonitrile (40:60). The flow rate was maintained at 1.0 mL x min(-1) with the column temperature at ambient conditions. The detection wavelength was set at 350 nm.
  
  
  RESULTS
  The average content of Z-ligustilide in 21 CX samples was found to be 7.40 +/- 3.54 mg x g(-1)(x +/- s, n = 21). Therefore,the content of Z-ligustilide in CX should not be less than 0.66% (calculated on the dried basis).
  
  
  CONCLUSIONS
  The overall analytical procedure is rapid and accuracy which is considered suitable for the quantitative analysis of ligustilide in CX. The amount of ligustilide in CX samples collected from different cultivation areas was obviously different. However, a relatively higher content of ligustilide was generally found in the CX collected from its main cultivated areas.
authors.affiliation: Sichuan Provincial Institute for Food and Drug Control, Chengdu 610036, China.
authors.forename: Shi-Qiong
authors.initials: SQ
authors.lastname: Cheng
authors.forename: Guang-Hua
authors.initials: GH
authors.lastname: Lv
authors.forename: Shi-Xian
authors.initials: SX
authors.lastname: Liang
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.forename: Yu-Cong
authors.initials: YC
authors.lastname: Xu
authors.forename: Zhong-Zhen
authors.initials: ZZ
authors.lastname: Zhao
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D017753:Ecosystem; D030004:Ligusticum / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry
chemical_list: C027820:ligustilide; D015107:4-Butyrolactone