title: NMR-based metabolic profiling of human hepatoma cells in relation to cell growth by culture media analysis.
abstract: Metabolic profiling is a metabolomic approach that allows the characterization of metabolic phenotypes under specific set of conditions. In the present paper we investigated the metabolism of sparse and high density cultures in relation to different cell growth phases. Changes in the metabolome were evaluated by using 1H-NMR spectroscopy, correlation map and Multivariate Data Analysis on the net balances of metabolites in the medium. This approach allowed us to identify two different metabolic profiles in relation to the cell growth phases in subconfluence and confluence cultures. The results have been interpreted on the basis of patterns of correlations obtained in the two physiological cell states. Cells almost arrested in G0/G1 phase by contact dependent growth inhibition underwent changes in the channeling of amino acids utilization from synthetic to energetic purpose and in anaplerosis/cataplerosis regulation of the TCA cycle.
authors.affiliation: Department of Chemistry, University La Sapienza, P.le Aldo Moro 5-00185 Rome, Italy. alfredo.miccheli@uniromal.it
authors.forename: Alberta Tomassini
authors.initials: AT
authors.lastname: Miccheli
authors.forename: Alfredo
authors.initials: A
authors.lastname: Miccheli
authors.forename: Roberta
authors.initials: R
authors.lastname: Di Clemente
authors.forename: Mariacristina
authors.initials: M
authors.lastname: Valerio
authors.forename: Pierpaolo
authors.initials: P
authors.lastname: Coluccia
authors.forename: Mariano
authors.initials: M
authors.lastname: Bizzarri
authors.forename: Filippo
authors.initials: F
authors.lastname: Conti
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle / Q000502:physiology; D049109:Cell Proliferation; D003470:Culture Media; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D014407:Tumor Cells, Cultured
chemical_list: D003470:Culture Media