Functional characterization of the promoter for the mouse SPTLC2 gene, which encodes subunit 2 of serine palmitoyltransferase.
A series of luciferase reporter constructs was prepared from a 1035-bp fragment of mouse genomic DNA flanking the 5'-coding sequence for the SPTLC2 subunit of serine palmitoyltransferase, the initial enzyme of de novo sphingolipid biosynthesis. The full-length DNA fragment promoted strong reporter gene expression in NIH3T3 cells while deletion and site-directed mutagenesis indicated that the proximal 335 bp contain initiator and downstream promoter elements, two proximal GC boxes that appear to stimulate transcription in a cooperative manner, and several additional elements whose activity cannot be accounted for by known factor binding sites. These findings provide insight into the control mechanisms for transcription of mammalian SPTLC2.
Linn
Stephen C
SC
School of Biology and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Andras
Lindsay M
LM
Kim
Hee-Sook
HS
Wei
Jia
J
Nagiec
M Marek
MM
Dickson
Robert C
RC
Merrill
Alfred H
AH
D000818:Animals; D001665:Binding Sites; D017930:Genes, Reporter; D051379:Mice; D016296:Mutagenesis; D041681:NIH 3T3 Cells; D011401:Promoter Regions, Genetic; D021122:Protein Subunits / Q000235:genetics; D050436:Regulatory Elements, Transcriptional; D051102:Serine C-Palmitoyltransferase / Q000235:genetics*; D014158:Transcription, Genetic; D014161:Transduction, Genetic
D021122:Protein Subunits; C495089:SPTLC2 protein, human; D051102:Serine C-Palmitoyltransferase