title: TOPOFIT-DB, a database of protein structural alignments based on the TOPOFIT method.
abstract: TOPOFIT-DB (T-DB) is a public web-based database of protein structural alignments based on the TOPOFIT method, providing a comprehensive resource for comparative analysis of protein structure families. The TOPOFIT method is based on the discovery of a saturation point on the alignment curve (topomax point) which presents an ability to objectively identify a border between common and variable parts in a protein structural family, providing additional insight into protein comparison and functional annotation. TOPOFIT also effectively detects non-sequential relations between protein structures. T-DB provides users with the convenient ability to retrieve and analyze structural neighbors for a protein; do one-to-all calculation of a user provided structure against the entire current PDB release with T-Server, and pair-wise comparison using the TOPOFIT method through the T-Pair web page. All outputs are reported in various web-based tables and graphics, with automated viewing of the structure-sequence alignments in the Friend software package for complete, detailed analysis. T-DB presents researchers with the opportunity for comprehensive studies of the variability in proteins and is publicly available at http://mozart.bio.neu.edu/topofit/index.php.
authors.affiliation: Department of Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
authors.forename: Chesley M
authors.initials: CM
authors.lastname: Leslin
authors.forename: Alexej
authors.initials: A
authors.lastname: Abyzov
authors.forename: Valentin A
authors.initials: VA
authors.lastname: Ilyin
mesh_terms: D030562:Databases, Protein; D020407:Internet; D008432:Mathematical Computing; D040681:Structural Homology, Protein; D014584:User-Computer Interface