Influence of platelet-rich plasma on osteogenic differentiation of mesenchymal stem cells and ectopic bone formation in calcium phosphate ceramics.
Platelet-rich plasma (PRP) contains a mixture of growth factors that play an important role in wound and fracture healing. While PRP enhanced bone formation by autogenous cancellous bone grafts, its influence in combination with different bone substitutes remained unknown. This study evaluated the effect of PRP on osteogenic differentiation and ectopic bone formation of human mesenchymal stem cells (MSC) in distinct resorbable calcium phosphate ceramics. Calcium-deficient hydroxyapatite (CDHA) blocks with a large specific surface area (48 m2/g) and beta-tricalcium phosphate (beta-TCP) with a low specific surface area (<0.5 m2/g) were loaded with 2 x 10(5) bone marrow-derived MSC. Half of the specimens were treated with 5-fold concentrated PRP. Biocomposites were implanted subcutaneously into SCID mice or kept under osteogenic culture conditions for 2 weeks before implantation. The addition of PRP increased the specific alkaline phosphatase (ALP) activity (p = 0.012) in undifferentiated MSC/CDHA composites but not in MSC/beta-TCP composites. Osteogenic preinduction was ineffective for CDHA and reduced ALP activity of beta-TCP composites significantly at explantation. Ectopic bone formation was stronger in MSC/CDHA (7/32) compared to MSC/beta-TCP (2/30) composites, but no influence of PRP was evident. In conclusion, the effect of PRP depended on the type of ceramic and the differentiation status of the MSC, and enhanced ALP activity of MSC on the high surface scaffold CDHA only, but PRP did not improve osteogenesis in our setting.
Kasten
Philip
P
Division of Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg, Heidelberg, Germany.
Vogel
Julia
J
Luginbühl
Reto
R
Niemeyer
Philipp
P
Weiss
Stefan
S
Schneider
Sven
S
Kramer
Martin
M
Leo
Albrecht
A
Richter
Wiltrud
W
D000368:Aged; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001792:Blood Platelets / Q000378:metabolism*; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000138:chemical synthesis*; D001842:Bone and Bones / Q000378:metabolism*; D002130:Calcium Phosphates / Q000737:chemistry*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry; D005260:Female; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D010006:Osteoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000032:analysis; D013499:Surface Properties; D013997:Time Factors; D023822:Tissue Engineering
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D015675:Osteocalcin; D017886:Durapatite; D000469:Alkaline Phosphatase