Substrate consumption and biomass growth of Ralstonia eutropha at various S0/X0 levels in batch cultures.
The biomass growth, substrate consumption and polyhydrobutyrate (PHB) production of Ralstonia eutropha with butyric acid and fructose as the carbon and energy sources at various ratios of initial substrate concentration (S0) to initial biomass concentration (X0) were investigated in this study. Results indicated that the PHB content increased with the increasing S0/X0 ratio. Different substrates exhibited a similar trend for cell growth and substrates consumption with the changing S0/X0 ratio. The specific consumption rates of both butyric acid and fructose increased with the increasing S0/X0 ratio. An S0/X0-dependent kinetic model was modified to describe the kinetics of biomass growth and substrate consumption for R. eutropha. This model was verified with the experimental results from this work and in literature.
Wang
Jin
J
School of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Fang
Fang
F
Yu
Han-Qing
HQ
D018533:Biomass; D002455:Cell Division; D020118:Cupriavidus necator / Q000254:growth & development / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism*
D006885:Hydroxybutyrates; D005632:Fructose