Differences in binding of nuclear proteins from P. berghei strains differing by chloroquine resistance to oligonucleotides corresponding to mdr1 gene regulatory elements.
Proteins specifically reacting with AP1, MEF1, NF-IL6, and SP1 transcription factor binding sites were detected for the first time in nuclear extract of P. berghei (rodent malaria parasite) using gel retardation assay. P. berghei strains with different chloroquine resistance exhibited appreciable differences in the pattern of nuclear protein binding to the majority of the studied sites, which attests to changes in the plasmodial regulatory system during chloroquine resistance selection.
Pankova
T G
TG
Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Novosibirsk. pankt@yandex.ru
Igonina
T M
TM
Kobzev
V F
VF
Merkulova
T I
TI
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D001483:Base Sequence; D002738:Chloroquine / Q000494:pharmacology*; D017931:DNA Primers; D004351:Drug Resistance / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism*; D010962:Plasmodium berghei / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D015800:Protozoan Proteins / Q000235:genetics*
D018528:ATP-Binding Cassette Transporters; D000962:Antimalarials; D017931:DNA Primers; D009687:Nuclear Proteins; D015800:Protozoan Proteins; C059987:mdr gene protein, Plasmodium; D002738:Chloroquine