title: Evaluation of the rapid analyte measurement platform (RAMP) for the detection of Bacillus anthracis at a crime scene.
abstract: The aim of this study was to evaluate the accuracy and reliability of the rapid analyte measurement platform (RAMP) for presumptive identification of Bacillus anthracis spores. Test samples consisted of serial dilutions of spore preparations of several Bacillus species, including B. anthracis, which were tested, using the RAMP Anthrax test cartridge, according to the manufacturer's instructions. The fluorescence labelled antibody-antigen complexes were detected in the portable reader after 15 min following sample addition. Dilutions of common environmental and household powders were also tested to identify possible false positive results. B. anthracis spores were identified reliably in test samples containing more than 6000 spores. The test kits were highly specific, showing no cross reactivity with other Bacillus species or any environmental powders tested. The RAMP system for detection of B. anthracis spores, from environmental samples, showed consistent results under a variety of analytical conditions, enabling the trained user to provide a rapid, accurate preliminary risk assessment of a suspected bioterrorism incident.
authors.affiliation: Forensic Counter Terrorism and Disaster Victim Identification Unit, NSW Police Headquarters, Level 5 Tower B, 1 Charles Street, Parramatta, New South Wales 2150, Australia. hoil1reb@police.nsw.gov.au
authors.forename: Rebecca
authors.initials: R
authors.lastname: Hoile
authors.forename: Marion
authors.initials: M
authors.lastname: Yuen
authors.forename: Gregory
authors.initials: G
authors.lastname: James
authors.forename: Gwendolyn L
authors.initials: GL
authors.lastname: Gilbert
mesh_terms: D001408:Bacillus anthracis / Q000302:isolation & purification*; D023482:Bioterrorism; D005453:Fluorescence; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013171:Spores, Bacterial / Q000302:isolation & purification