qPrimerDepot: a primer database for quantitative real time PCR.
Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.
Cui
Wenwu
W
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, MD 20892-4605.
Taub
Dennis D
DD
Gardner
Kevin
K
D000818:Animals; D017931:DNA Primers; D030561:Databases, Nucleic Acid; D015870:Gene Expression; D006801:Humans; D020407:Internet; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014584:User-Computer Interface
D017931:DNA Primers