title: A balance of protein synthesis and proteasome-dependent degradation determines the maintenance of LTP.
abstract: Long-lasting changes in synaptic strength are thought to play a pivotal role in activity-dependent plasticity and memory. There is ample evidence indicating that in hippocampal long-term potentiation (LTP) the synthesis of new proteins is crucial for enduring changes. However, whether protein degradation also plays a role in this process has only recently begun to receive attention. Here, we examine the effects of blocking protein degradation on LTP. We show that pharmacological inhibition of proteasome-dependent protein degradation, just like inhibition of protein synthesis, disrupts expression of late (L-)LTP. However, when protein degradation and protein synthesis are inhibited at the same time, LTP is restored to control levels, calling into question the commonly held hypothesis that synthesis of new proteins is indispensable for L-LTP. Instead, these findings point to a more facetted model, in which L-LTP is determined by the combined action of synthesis and degradation of plasticity proteins.
authors.affiliation: Department of Cellular and Systems Neurobiology, Max Planck Institute of Neurobiology, Am Klopferspitz 18, 82152 München-Martinsried, Germany.
authors.forename: Rosalina
authors.initials: R
authors.lastname: Fonseca
authors.forename: Ramunas M
authors.initials: RM
authors.lastname: Vabulas
authors.forename: F Ulrich
authors.initials: FU
authors.lastname: Hartl
authors.forename: Tobias
authors.initials: T
authors.lastname: Bonhoeffer
authors.forename: U Valentin
authors.initials: UV
authors.lastname: Nägerl
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009924:Organ Culture Techniques; D017729:Presynaptic Terminals / Q000187:drug effects / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000187:drug effects / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004791:Enzyme Inhibitors; D009419:Nerve Tissue Proteins; D011500:Protein Synthesis Inhibitors; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex