[Study on the effect and its mechanisms of genistein on the cell cycle of highly metastatic ovarian carcinoma HO-8910PM cells].
OBJECTIVE
To study the effect and its possible mechanism of genistein on the cell cycle of human highly metastatic ovarian carcinoma HO-8910PM cells.


METHODS
Trypan blue stain assay was used to examine the effect of genistein on proliferation of HO-8910PM cells after 24 hours treatment. The cell cycle was assessed by flowcytometry (FCM). The expression level of NF-kappaB (p65) and the level of VEGF were assessed by Western blot analysis.


RESULTS
Genistein could inhibit the proliferation of HO-8910PM cell and block the cell cycle at G1 phase. The expession level of NF-kappaB (P65) protein decreased obviously in HO-8910PM cells treated with 25 approximately 100 micromol/L genistein for 24 hours, and the effect appeared in the experssion of VEGF.


CONCLUSIONS
The effect on cell cycle of genistein is involved in the decreasing expression of NF kappaB (p65) and the level of VEGF.
Ma
Wei-lie
WL
Department of Biochemistry, Guangdong Medical College, Zhanjiang, China.
Ding
Hang
H
Fu
Wei-yu
WY
Zhou
Ke-yuan
KY
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D005260:Female; D005434:Flow Cytometry; D019833:Genistein / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D016328:NF-kappa B / Q000096:biosynthesis; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D010946:Plants, Medicinal / Q000737:chemistry; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
D000972:Antineoplastic Agents, Phytogenic; D016328:NF-kappa B; D042461:Vascular Endothelial Growth Factor A; D019833:Genistein