[Study on therapeutic mechanisms of Danshen injection in intrahepatic cholestasis of pregnancy].
OBJECTIVE
To study the therapeutic mechanisms of Danshen injection in intrahepatic cholestasis of pregnancy (ICP).


METHODS
The experimental serum was collected from 6 patients with ICP before therapy when in hospital. Endothelial cells were isolated from human umbilical veins chosen among the 10 normal pregnancy according to Jaffe's method. Serum of 20% concentration of ICP and different concentration of Danshen injection were cocultured with human vascular endothelial cells (HUVECs) for 24 hours. MTT method was applied to measuring the vitality of HUVECs. The expression value of vascular endothelial growth factor (VEGF) of different groups was detected by immunocytochemistry and enzyme-linked immunosorbent assay (ELISA) respectively.


RESULTS
Compared with ICP group, the value of MTT and VEGF in the Danshen injection therapeutic groups were increased with the increase of concentration and moreover, 8 g x L(-1) of Danshen injection was the optical concentration. By immunocytochemistry, the expression of VEGF in HUVECs in the Danshen injection therapeutic groups was also enhanced with the increase of concentration.


CONCLUSIONS
Danshen injection can protect HUVECs against the injury of ICP serum and promote the expression of VEGF. Danshen injection improves HUVECs by increasing the value of VEGF.
Du
Xue-han
XH
The Clinical Medical School of Southeast University, Nanjing 210009, China.
Jiang
Li
L
Jiang
Xiao-qing
XQ
D002478:Cells, Cultured; D002780:Cholestasis, Intrahepatic / Q000097:blood*; D018920:Coculture Techniques; D003593:Cytoplasm / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007267:Injections; D010946:Plants, Medicinal / Q000737:chemistry; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood; D020011:Protective Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D027561:Salvia miltiorrhiza / Q000737:chemistry; D044967:Serum / Q000502:physiology; D014471:Umbilical Veins / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D042461:Vascular Endothelial Growth Factor A