title: Use of optophoresis as an in vitro predictor of cell response to chemotherapy for chronic lymphocytic leukemia.
abstract: B-cell chronic lymphocytic leukemia [CLL] is characterized by active accumulation of clonal CD5+/CD19+/CD23+ B cells. Individualized characterization of patient cell resistance/sensitivity to specific agents can provide important information to guide therapy selection. We have utilized optophoresis, which is a technique for the analysis of the motion of cells within a moving optical gradient field. It detects the broad cellular changes associated with apoptosis based on physical characteristics of the cell, such as morphology, size, refractive index, density, and surface properties. We analyzed peripheral blood samples from 62 CLL patients in the presence of varying concentrations of chemotherapeutic agents. Optophoresis and a more conventional measurement of cell death were utilized. The outcome of ex vivo drug resistance using optophoresis was compared to clinical response in 30 patients for which there was clinical outcome data available. The overall accuracy of optophoresis in reflecting clinical response was 80%. It has advantages over alternative methods of determining chemoresistance including the ability to evaluate very small sample sizes and ability to work in mixed-cell populations. Changes in cell physical characteristics in response to chemotherapy, as measured by optophoresis is an accurate method for predicting chemosensitivity ex vivo in CLL.
authors.affiliation: Genoptix, Inc., 3398 Carmel Mountain Road, San Diego, CA 92121, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Nerenberg
authors.forename: Ilona
authors.initials: I
authors.lastname: Kariv
authors.forename: Patricia
authors.initials: P
authors.lastname: McNeeley
authors.forename: Philippe
authors.initials: P
authors.lastname: Marchand
authors.forename: Sudipto
authors.initials: S
authors.lastname: Sur
authors.forename: Jonathan
authors.initials: J
authors.lastname: Diver
authors.forename: Samuel
authors.initials: S
authors.lastname: Riccitelli
authors.forename: Jorge
authors.initials: J
authors.lastname: Nieva
authors.forename: Alan
authors.initials: A
authors.lastname: Saven
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D016903:Drug Monitoring; D004354:Drug Screening Assays, Antitumor; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000188:drug therapy* / Q000473:pathology*; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D018715:Microscopy, Video / Q000379:methods*; D010812:Physical Stimulation / Q000295:instrumentation* / Q000379:methods; D012372:ROC Curve; D012680:Sensitivity and Specificity; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D000255:Adenosine Triphosphate