Reactive oxygen species production is involved in quercetin-induced apoptosis in human hepatoma cells.
Hepatocellular carcinoma (HCC) is the leading cause of cancer mortality in Asia. The aim of this study was to examine whether reactive oxygen species production is involved in quercetin-induced apoptosis in human HCC cell lines. Quercetin inhibited the growth of hepatoma cells in dose and time dependent manners. Quercetin treatment of hepatoma cells resulted in changes of cell cycle progression. The G0/G1phase was decreased and S phase was increased in HA22T/VGH cells after treatment with quercetin. The levels of apoptotic sub-G0/G1, reactive oxygen species and annexin V were increased prior to cell death and concurrent with lipid peroxidation in two human hepatoma cells after treatment with quercetin. Quercetin also enhanced the apoptotic effect of the chemotherapeutic agent, paclitaxel, in HA22T/VGH cells. Quercetin has therapeutic potential as an anti-cancer drug. These results provide basis for further study into the potential use of quercetin in combination with paclitaxel for treatment of hepatoma.
Chang
Yuh-Fang
YF
Department of Medical Research and Education, Taipei Veternas General Hospital, Taiwan.
Chi
Chin-Wen
CW
Wang
Jane-Jen
JJ
D017304:Annexin A5 / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000493:pharmacokinetics; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D017239:Paclitaxel / Q000493:pharmacokinetics; D011794:Quercetin / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
D017304:Annexin A5; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D011794:Quercetin; D017239:Paclitaxel