[Nitric oxide synthase activity and nitrates/nitrites level in brain regions during the spontaneous morphine withdrawal in rats].
Nitric oxide synthase (NOS) activity and nitrate/nitrites (NO(x)-) concentrations were measured in brain regions of rats during the spontaneous morphine withdrawal. Male Wistar rats were injected intraperotoneally with morphine hydrochloride at increasing doses (10-100 mg/kg) during 6 days twice a day. Thirty six hours after the last injection the severity of the spontaneous morphine withdrawal syndrome was determined by specific autonomic and locomotor indices. Both NOS activity and NO(x)- levels increased in the midbrain and the hippocampus, decreased in the striatum and the hypothalamus, and did not change in the cerebral cortex and the brain stem. NO(x)- concentrations in the cerebellum did not change, while NOS activity decreased. Both NOS activities and NO(x)- concentrations in the cerebral cortex, striatum, midbrain, and cerebellum correlated with withdrawal syndrome severity on the whole, and with the specific signs of abstinence.
Peregud
D I
DI
Onufriev
M V
MV
Iakovlev
A A
AA
Stepanichev
M Iu
MIu
Lazareva
N A
NA
Pavlova
T V
TV
Panchenko
L F
LF
Guliaeva
N V
NV
D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009020:Morphine / Q000009:adverse effects*; D009043:Motor Activity; D009566:Nitrates / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D013375:Substance Withdrawal Syndrome / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology
D009566:Nitrates; D009573:Nitrites; D009020:Morphine; D019001:Nitric Oxide Synthase