[Study on separation and purification of anthraquinones in radix et rhizoma rhei by D301 macroporous resin].
OBJECTIVE
The parameters of absorption and purification of total anthraquinones in Radix Et Rhizoma Rhei by D301 macroporous resin were investigated in this paper.


METHODS
HPLC was used to analyze the content of total anthraquinones in Radix Et Rhizoma with emodin as control.


RESULTS
The appropriate adsorption conditions were: concentration of extract 0.5 g x mL(-1); pH 9; flow rate 1 BV x h(-1). When the 75% ethanol was used as elution and the concentration of HCl was 0.1 mol x L(-1), and the flow rate was 1.0 BV x h(-1), the effect of desorption was satisfactory.


CONCLUSIONS
D301 resin provided a good effect on the exchang and absorption of total anthraquinones in Radix Et Rhizoma.
Xu
Han-Lin
HL
Hubei College of Traditional Chinese Medicine, Wuhan 430061, China. xhl4201@sina.com
Chen
Jun
J
Shao
Ji-Zhen
JZ
Sun
Yun
Y
Zhang
Chi-Zhi
CZ
D000880:Anthraquinones / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic; D012250:Rheum / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry
D000880:Anthraquinones; D012117:Resins, Synthetic