title: Phosphopeptide enrichment by IEF.
abstract: In our efforts to improve the identification of phosphopeptides by MS we have used peptide IEF on IPG strips. Phosphopeptides derived from trypsin digests of single proteins as well as complex cellular protein mixtures can be enriched by IEF and recovered in excellent yields at the acidic end of an IPG strip. IPG peptide fractionation in combination with MS/MS analysis has allowed us to identify phosphopeptides from tryptic digests of a cellular protein extract.
authors.affiliation: Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Maccarrone
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Kolb
authors.forename: Larysa
authors.initials: L
authors.lastname: Teplytska
authors.forename: Isabel
authors.initials: I
authors.lastname: Birg
authors.forename: Richard
authors.initials: R
authors.lastname: Zollinger
authors.forename: Florian
authors.initials: F
authors.lastname: Holsboer
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Turck
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D051379:Mice; D010748:Phosphopeptides / Q000302:isolation & purification*; D010766:Phosphorylation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D018892:Proton-Motive Force; D011972:Receptor, Insulin / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000032:analysis; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000378:metabolism
chemical_list: D010748:Phosphopeptides; D011506:Proteins; D011993:Recombinant Fusion Proteins; D011972:Receptor, Insulin; D014357:Trypsin