Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1.
A newly isolated strain of Kluyveromyces marxianus YS-1 was used for the production of extra cellular inulinase in a medium containing inulin, meat extract, CaCl2 and sodium dodecyl sulphate (SDS). Fermentation medium pH 6.5, cultivation temperature 30 degrees C and 5% (v/v) inoculum of 12 h-old culture were optimal for enzyme production (30.8 IU/ml) with a fermentation time of 72 h at shake flask level. Raw inulin (2%, w/v) extracted from dahlia tubers by processing at 15 kg/cm2 for 10 min was optimum for bioreactor studies. Maximum enzyme production (55.4 IU/ml) was obtained at an agitation rate of 200 rpm and aeration of 0.75 vvm in a stirred tank reactor with a fermentation time of 60 h.
Singh
R S
RS
Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala 147 002, India. rsingh11@lycos.com
Sooch
Balwinder Singh
BS
Puri
Munish
M
D019149:Bioreactors; D003470:Culture Media; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000302:isolation & purification; D007716:Kluyveromyces / Q000201:enzymology*; D009584:Nitrogen / Q000378:metabolism; D013501:Surface-Active Agents; D014131:Trace Elements / Q000378:metabolism
D003470:Culture Media; D013501:Surface-Active Agents; D014131:Trace Elements; D006026:Glycoside Hydrolases; C023086:inulinase; D009584:Nitrogen