title: Spatial codes in dendritic BC1 RNA.
abstract: BC1 RNA is a dendritic untranslated RNA that has been implicated in local translational control mechanisms in neurons. Prerequisite for a functional role of the RNA in synaptodendritic domains is its targeted delivery along the dendritic extent. We report here that the targeting-competent 5' BC1 domain carries two dendritic targeting codes. One code, specifying somatic export, is located in the medial-basal region of the 5' BC1 stem-loop structure. It is defined by an export-determinant stem-bulge motif. The second code, specifying long-range dendritic delivery, is located in the apical part of the 5' stem-loop domain. This element features a GA kink-turn (KT) motif that is indispensable for distal targeting. It specifically interacts with heterogeneous nuclear ribonucleoprotein A2, a trans-acting targeting factor that has previously been implicated in the transport of MBP mRNA in oligodendrocytes and neurons. Our work suggests that a BC1 KT motif encodes distal targeting via the A2 pathway and that architectural RNA elements, such as KT motifs, may function as spatial codes in neural cells.
authors.affiliation: Department of Physiology and Pharmacology, The Robert F. Furchgott Center for Neural and Behavioral Science, State University of New York Health Science Center at Brooklyn, Brooklyn, NY 11203, USA.
authors.forename: Ilham A
authors.initials: IA
authors.lastname: Muslimov
authors.forename: Anna
authors.initials: A
authors.lastname: Iacoangeli
authors.forename: Jürgen
authors.initials: J
authors.lastname: Brosius
authors.forename: Henri
authors.initials: H
authors.lastname: Tiedge
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism*; D029721:Drosophila Proteins; D004622:Embryo, Mammalian; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics; D008845:Microinjections; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012313:RNA; D034443:RNA Transport; D020733:RNA, Small Cytoplasmic / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017783:Superior Cervical Ganglion; D015534:Trans-Activators / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; C117734:BC1 RNA; D029721:Drosophila Proteins; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D018398:Homeodomain Proteins; D020733:RNA, Small Cytoplasmic; C039919:RNA, recombinant; D015534:Trans-Activators; C056478:bcd protein, Drosophila; C439768:hnRNP A2; D012313:RNA