title: Dynactin targets Pavarotti-KLP to the central spindle during anaphase and facilitates cytokinesis in Drosophila S2 cells.
abstract: The dynactin complex cooperates with the dynein complex in various systems for mitotic completion. Here we analysed the mitotic phenotype of Drosophila S2 cells following the knockdown of the dynactin subunit p150(Glued). We found that p150(Glued)-depleted cells were delayed in metaphase and that the centrosomes were poorly connected to mitotic spindle poles. In addition, anaphase occurred with asynchronous chromosome segregation. Although cyclin B was degraded in these anaphase cells, Aurora B, MEI-S322 and BubR1 were not released from the non-segregating chromosomes. We also found that the density and organisation of the central spindle were compromised, with Aurora B and polo kinases absent from the diminished number of microtubules. Pavarotti-KLP, a component of the centralspindlin complex required for the formation of stable microtubule bundles, was not immediately targeted to the plus ends of the microtubules following anaphase onset as happened in controls. Instead, it accumulated transiently at the cell cortex during early anaphase and its targeting to the central spindle was delayed. These data suggest that the dynactin complex contributes to cytokinesis by promoting stable targeting of the centralspindlin complex to microtubule plus ends at anaphase onset. The contribution of the dynein-dynactin complex to synchronous chromosome segregation and cytokinesis is discussed.
authors.affiliation: CNRS UMR 6061 Génétique et Développement, Groupe Cycle Cellulaire, Faculté de Médecine, IFR 140 Génomique Fonctionnelle et Santé, Université de Rennes I, 2 avenue du Pr. Léon Bernard, CS 34317, F-35043 Rennes CEDEX, France.
authors.forename: Jean-Guy
authors.initials: JG
authors.lastname: Delcros
authors.forename: Claude
authors.initials: C
authors.lastname: Prigent
authors.forename: Régis
authors.initials: R
authors.lastname: Giet
mesh_terms: D000705:Anaphase / Q000502:physiology*; D000818:Animals; D064127:Aurora Kinases; D015153:Blotting, Western; D002478:Cells, Cultured; D018385:Centrosome / Q000378:metabolism; D020090:Chromosome Segregation; D019926:Cyclin B / Q000378:metabolism; D056744:Cyclin B1; D048749:Cytokinesis / Q000502:physiology*; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D000072159:Dynactin Complex; D004398:Dyneins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D008869:Microtubule-Associated Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D008941:Spindle Apparatus / Q000502:physiology*
chemical_list: D019926:Cyclin B; D056744:Cyclin B1; D029721:Drosophila Proteins; D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D034741:RNA, Small Interfering; C490516:pav protein, Drosophila; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases; D004398:Dyneins