title: Genetic variation of Chinese PRRSV strains based on ORF5 sequence.
abstract: Thirteen isolates of porcine reproductive and respiratory syndrome virus (PRRSV) from different provinces of China were studied and compared with several PRRSV isolates from other countries. Phylogenetic analysis shows that all Chinese isolates of PRRSV in this study belong to the American genotype, except for one strain, B13, which clustered as a European genotype. Sequence analysis revealed that PRRSV Chinese isolates of the American genotype were highly similar in the ORF5 sequence and could be classified into two subclades. One contains PRRSV isolates that are more closely related to the American vaccine strain MLV Resp and its parent strain VR-2332, and the other contains ones only distantly related to them. Within the Chinese isolates slight genetic variation occurred, and some strains may originate directly from the vaccine virus.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Cheng-Gang
authors.initials: CG
authors.lastname: Zhu
authors.forename: Yong-Feng
authors.initials: YF
authors.lastname: Jin
authors.forename: Yao-Zhou
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D005814:Genes, Viral; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers