[Effects of quercetin on angiotensin II induced interleukin-6 in vascular smooth muscle cells].
OBJECTIVE
To observe the effects of quercetin on angiotensin (Ang II) induced interleukin-6 (IL-6) in vascular smooth muscle cells (VSMCs).


METHODS
VSMCs were isolated from the thoracic aorta of Sprague-Dawley rats and were stimulated with different doses of Ang II. The production of IL-6 in supernatant of quercetin treated cultures was detected by ELISA. In parallel, IL-6 mRNA level was measured by RT-PCR.


RESULTS
Ang II induced a marked increase of IL-6 in a dose- and time-dependent manner in the culture of VSMCs. Quercetin inhibited the production of Ang II -induced IL-6 in the culture in a dose-dependent manner. Similarly, the result with RT-PCR indicated that the expression of IL-6 mRNA induced by Ang II for 24h was down-regulated by quercetin.


CONCLUSIONS
It demonstrates that quercetin possesses a inhibition of Ang II-induced production of IL-6 in VSMCs. Moreover, quercetin also down regulates the expression of IL-6 mRNA, suggesting the action of quercetin on IL-6 release induced by Ang II in VSMCs may underlie its anti-inflammatory properties.
Peng
Ning
N
Department of Pharmacology, Xi'an Jiao University of Medicine, China.
Liu
Juan-tian
JT
Lin
Rong
R
D000804:Angiotensin II / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry*; D011794:Quercetin / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D012333:RNA, Messenger; D000804:Angiotensin II; D011794:Quercetin