title: [Effect of polysaccharides content of tissue culturing seedlings on Dendrobium candidium under sound wave stimulation].
abstract: OBJECTIVE
  To detect the polysaccharides content of tissue culturing seedlings on Dendrobium candidium under special sound wave stimulation.
  
  
  METHODS
  The content of polysaccharides was detected by phenol-vitriol colorimetry method.
  
  
  RESULTS
  The polysaccharides content of the groups stimulated continuously for 24d was higher obviously than the content of the control groups.
  
  
  CONCLUSIONS
  The stimulation of the special sound wave promoted markedly the synthesis of polysaccharides in D. candidium. It may affect obviously the metabolic pathway of polysaccharides in D. candidium.
authors.affiliation: Key Laboratory for Biomechanics and Tissue Engineering under the State Ministry of Education, College of Bioengineering, Chongqing University, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Li
authors.forename: Bo-chu
authors.initials: BC
authors.lastname: Wang
authors.forename: Yi-long
authors.initials: YL
authors.lastname: Liang
authors.forename: Kun
authors.initials: K
authors.lastname: Tang
authors.forename: Kun-xian
authors.initials: KX
authors.lastname: Shu
authors.forename: Wan-qian
authors.initials: WQ
authors.lastname: Liu
mesh_terms: D003124:Colorimetry / Q000379:methods; D046508:Culture Techniques / Q000379:methods; D031667:Dendrobium / Q000737:chemistry / Q000254:growth & development*; D010788:Photosynthesis; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development*; D011134:Polysaccharides / Q000302:isolation & purification / Q000378:metabolism*; D036226:Seedlings / Q000737:chemistry / Q000254:growth & development; D013016:Sound; D013997:Time Factors
chemical_list: D011134:Polysaccharides