title: Polymorphisms of TGF-beta1 in cystic fibrosis patients.
abstract: There is a significant phenotypic variance among cystic fibrosis (CF) patients. Due to the role of TGF-beta1 in fibrotic processes we investigated its role in CF pathogenesis. TGF-beta 1 codons 10 and 25 were genotyped in 118 Czech CF patients and 268 controls by PCR-ARMS. Difference between CF and controls was found at codon 10, lower frequency of T/T homozygotes, and codon 25, higher frequency of G/C heterozygotes. We did not prove the association of TGF-beta1 polymorphisms and lung function in CF, however, the TT (codon 10)/GG (codon 25) genotype was preferentially associated with CF-related liver disease and diabetes. Independent of the TGF-beta1 genotype, production of cytokine was higher in patients than in controls with the notable exception of very low levels in Burkholderia cepacia complex colonized patients. In CF, both extremes, highest or lowest TGF-beta 1 production, were associated with impaired lung function.
authors.affiliation: Institute of Immunology, University Hospital Motol, Charles University, Prague, V Uvalu 84, 150 06 Prague 5, Czech Republic. jitka.brazova@centrum.cz
authors.forename: Jitka
authors.initials: J
authors.lastname: Brazova
authors.forename: Kristyna
authors.initials: K
authors.lastname: Sismova
authors.forename: Vera
authors.initials: V
authors.lastname: Vavrova
authors.forename: Jana
authors.initials: J
authors.lastname: Bartosova
authors.forename: Milan
authors.initials: M
authors.lastname: Macek
authors.forename: Hynek
authors.initials: H
authors.lastname: Lauschman
authors.forename: Anna
authors.initials: A
authors.lastname: Sediva
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018153:Czech Republic / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D053773:Transforming Growth Factor beta1