title: Structure of internalin C from Listeria monocytogenes.
abstract: The crystal structure of internalin C (InlC) from Listeria monocytogenes has been determined at 2.0 A resolution. Several observations implicate InlC in infection: inlC has the same transcriptional activator as other virulence genes, it is only present in pathogenic Listeria strains and an inlC deletion mutant is significantly less virulent. While the extended concave receptor-binding surfaces of the leucine-rich repeat (LRR) domains of internalins A and B have aromatic clusters involved in receptor binding, the corresponding surface of InlC is smaller, flatter and more hydrophilic, suggesting that InlC may be involved in weak or transient associations with receptors; this may help explain why no receptor has yet been discovered for InlC. In contrast, the Ig-like domain, to which the LRR domain is fused, has surface aromatics that may be of functional importance, possibly being involved in binding to the surface of the bacteria or in receptor binding.
authors.affiliation: School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, England.
authors.forename: Amy
authors.initials: A
authors.lastname: Ooi
authors.forename: Syeed
authors.initials: S
authors.lastname: Hussain
authors.forename: Arefeh
authors.initials: A
authors.lastname: Seyedarabi
authors.forename: Richard W
authors.initials: RW
authors.lastname: Pickersgill
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D018360:Crystallography, X-Ray / Q000379:methods; D008089:Listeria monocytogenes / Q000737:chemistry* / Q000235:genetics / Q000472:pathogenicity; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; C095768:inlB protein, Listeria monocytogenes; C103178:inlC protein, Listeria monocytogenes