A ginseng-specific abundant protein (GSAP) located on the cell wall is involved in abiotic stress tolerance.
Ginseng ESTs allowed us to identify an unknown transcript which is highly abundant in rhizomes and seeds. We called the cDNA ginseng-specific abundant protein (GSAP), and identified three homologues, GSAP1, GSAP2, and GSAP3. GSAP cDNAs encode a small polypeptide consisting of 121 or 117 amino acids, and GSAP3 shows 87.6% amino acid sequence homology with GSAP1. GSAP transcripts were detected in most plant tissues, but GSAP3 is highly expressed in seeds, and is up-regulated under stressed conditions, water deficit. GSAP3-GFP fusion protein is located in the cell wall when expressed in onion epidermis cells. The transgenic Arabidopsis seedlings which over-expressed GSAP3 grew faster than those of the wild-type plant on the medium containing 300 mM mannitol and 100 mM NaCl. GSAP3 may play a role in altering the characteristics of the cell wall to allow for more tolerance of water deficit stress under abiotic stress conditions.
Ha
Young Im
YI
Eugentech Inc. 52 Oun-Dong, Yusong, Daejon 305-333, Republic of Korea.
Lim
Jong Min
JM
Ko
Suk-Min
SM
Liu
Jang Ryol
JR
Choi
Dong-Woog
DW
D000222:Adaptation, Physiological / Q000235:genetics; D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology; D002473:Cell Wall / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D003681:Dehydration / Q000378:metabolism; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000502:physiology*; D005894:Panax / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D020033:Protein Isoforms