PCR detection of foodborne bacteria producing the biogenic amines histamine, tyramine, putrescine, and cadaverine.
This study describes an easy PCR method for the detection of foodborne bacteria that potentially produce histamine, tyramine, putrescine, and cadaverine. Synthetic oligonucleotide pairs for the specific detection of the gene coding for each group of bacterial histidine, tyrosine, ornithine, or lysine decarboxylases were designed. Under the conditions used in this study, the assay yielded fragments of 372 and 531 bp from histidine decarboxylase-encoding genes, a 825-bp fragment from tyrosine decarboxylases, fragments of 624 and 1,440 bp from ornithine decarboxylases, and 1,098- and 1,185-bp fragments from lysine decarboxylases. This is the first PCR method for detection of cadaverine-producing bacteria. The method was successfully applied to several biogenic amine-producing bacterial strains.
de las Rivas
Blanca
B
Departamento de Microbiología, Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.
Marcobal
Angela
A
Carrascosa
Alfonso V
AV
Muñoz
Rosario
R
D001431:Bacteriological Techniques; D001679:Biogenic Amines / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D002103:Cadaverine / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D053938:DNA Fragmentation; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006090:Gram-Negative Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D006632:Histamine / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011700:Putrescine / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D013045:Species Specificity; D014439:Tyramine / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification
D001679:Biogenic Amines; D006632:Histamine; D002103:Cadaverine; D011700:Putrescine; D014439:Tyramine