[Fluorescence characteristic of terbium-ciprofloxacin complex and its application].
On binding to deoxyribonucleic acid, the complex of terbium-ciprofloxacin (Tb(3+)-CIP) increases its fluorescence quantum efficiency. Based on this, an easy, rapid and sensitive method for the determination of DNA was developed. Like ethidium bromide (EB), the complex can be intercalated into DNA bases, but it is non-poisonous. Determination can be made at pH 7.0, where the native structure of DNA is not disrupted. The maximum emission is at 545 nm with excitation at 325 nm. This method has good sensitivity (2.8 x 10(-9) mol x L(-1) of ctDNA), high selectivity and a wide linear range (4.3 x 10(-7)-3. 0 x 10(-5) mol x L(-1) of ctDNA). This complex was also employed for the clinical examination of DNA in whole blood of tumor patients. Ten tumor patients and 10 normal persons were enrolled in this study. It was showed that significant difference existed between control group and tumor group (p < 0.05).
He
Hua
H
China Pharmaceutical University, Nanjing 210009, China.
Wang
Ling-Li
LL
Liu
Xu-Hui
XH
Jiao
Qing-Cai
QC
Chuong
Pham-Huy
PH
Wang
Guang-Ji
GJ
D000818:Animals; D002417:Cattle; D002939:Ciprofloxacin / Q000737:chemistry*; D004247:DNA / Q000097:blood / Q000737:chemistry*; D004273:DNA, Neoplasm / Q000097:blood / Q000737:chemistry; D004996:Ethidium / Q000737:chemistry; D005453:Fluorescence; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013050:Spectrometry, Fluorescence / Q000379:methods; D013725:Terbium / Q000737:chemistry*
D004273:DNA, Neoplasm; D013725:Terbium; D002939:Ciprofloxacin; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium