Evaluation of an ELISA rapid device for the serological diagnosis of Leishmania infantum infection in dog as compared with immunofluorescence assay and Western blot.
In this study we compared a commercial enzyme linked immunosorbent assay (ELISA) rapid test (Snap CLATK Canine Leishmania Antibody Test Kit, IDEXX-Snap) with indirect immunofluorescence assay (IFA) and Western blot (WB) for the detection of Leishmania infantum antibodies in dogs. In total sera from 234 dogs were collected: 59 positives and 51 doubtful sera (IFA 1:40-1:80) from an L. infantum endemic area and 124 negative sera from a non-endemic area were tested. To evaluate the Snap CLATK's performances on whole blood, blood in EDTA and sera from 37 dogs were tested in parallel with Snap CLATK. Snap CLATK sensitivity and specificity compared to IFA were 91.1% and 99.2%, while compared to WB were 93.4% and 98.3%, respectively. When IFA doubtful sera (titers of 1:40 or 1:80) were tested Snap CLATK, using WB as reference, sensitivity and specificity were 90.9% and 100%, respectively. Moreover, a complete concordance was observed when Snap CLATK rapid assay was carried out on whole blood or sera from 37 dogs. The Snap CLATK has demonstrated simplicity and performance and can be considered a quick and reliable alternative for the diagnosis of L. infantum infection in dogs.
Ferroglio
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E
Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università di Torino, Italy. ezio.ferroglio@unito.it
Centaro
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E
Mignone
W
W
Trisciuoglio
A
A
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D015153:Blotting, Western / Q000379:methods / Q000592:standards / Q000662:veterinary; D004283:Dog Diseases / Q000175:diagnosis* / Q000453:epidemiology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000592:standards / Q000662:veterinary; D007558:Italy / Q000453:epidemiology; D018314:Leishmania infantum / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan