Simultaneous analysis of nine active components in Gegen Qinlian preparations by high-performance liquid chromatography with diode array detection.
HPLC with diode array detection (HPLC/DAD) was employed to determine the quantities of puerarin, daidzin, daidzein, berberine, palmatine, coptisine, baicalin, baicalein, and glycyrrhizin in Gegen Qinlian preparations of three different pharmaceutical forms including decoction, dispensing granule and pill. The calibration curves for the nine bioactive components were linear in the given concentration ranges. The precision of the method was in the range of 0.2 - 5.0% (RSD), and the recoveries of this method were between 96.5 and 104.1%. The proposed method was applicable to analyze Gegen Qinlian preparations.
Chen
Lihong
L
Key Laboratory of Modern Traditional Chinese Medicine, Department of Chinese Meteria Medica Analysis, China Pharmaceutical University, Nanjing, PR China.
Wang
Qiang
Q
Liu
Jining
J
D001599:Berberine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D044950:Flavanones / Q000032:analysis; D007529:Isoflavones / Q000032:analysis; D015394:Molecular Structure; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D044950:Flavanones; D007529:Isoflavones; D001599:Berberine; C006680:baicalein; C033607:puerarin