1-Methylpyridinium-4-(4-phenylmethanethiosulfonate) iodide, MTS-MPP+, a novel scanning cysteine accessibility method (SCAM) reagent for monoamine transporter studies.
A novel substituted cysteine accessibility method (SCAM) reagent was developed for monoamine uptake transporters. The new reagent, MTS-MPP(+), was a derivative of the neurotoxin and transporter substrate MPP(+). MTS-MPP(+) labeled cysteine residues introduced into the serotonin transporter protein. Although it did not prove to be a substrate, as is MPP(+), it appears to label cysteine residues lining the permeation pore of the transporter more readily than currently available nonspecific SCAM reagents.
Gallardo-Godoy
Alejandra
A
Department of Medicinal Chemistry and Molecular Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Purdue University, West Lafayette, IN 47906, USA.
Torres-Altoro
Melissa I
MI
White
Kellie J
KJ
Barker
Eric L
EL
Nichols
David E
DE
D002460:Cell Line; D003545:Cysteine / Q000737:chemistry*; D006801:Humans; D007454:Iodides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D008958:Models, Molecular; D015394:Molecular Structure; D011726:Pyridinium Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D012701:Serotonin / Q000493:pharmacokinetics; D050486:Serotonin Plasma Membrane Transport Proteins / Q000737:chemistry / Q000187:drug effects*; D013329:Structure-Activity Relationship
C517093:1-methylpyridinium-4-(4-phenylmethanethiosulfonate) iodide; D007454:Iodides; D011726:Pyridinium Compounds; D050486:Serotonin Plasma Membrane Transport Proteins; D012701:Serotonin; D003545:Cysteine