title: Granulosa cell production of anti-Müllerian hormone is increased in polycystic ovaries.
abstract: BACKGROUND
  There has been renewed interest in anti-Müllerian hormone (AMH) because of its role in the ovary. Data on its actions are sparse, but it appears to inhibit follicle growth. Interestingly, serum AMH is two to three times higher in women with polycystic ovary (PCO) syndrome than women with normal ovaries.
  
  
  OBJECTIVE
  We examined the production of AMH by cells from a range of follicle sizes from normal ovaries and compared this with production by ovulatory and anovulatory (anov) PCOs.
  
  
  METHODS
  Granulosa cells (GCs) and theca and follicular fluid (ff) were isolated from intact follicles. Cells were cultured for 48 h +/- FSH or LH, and AMH was measured in ff and cell-conditioned media (CM).
  
  
  RESULTS
  AMH levels in ff and GC-CM ranged from 42 to 2240 and 0.025 to 1.7 ng/ml, respectively, and were low or undetectable in ff and GC-CM from follicles greater than 9 mm, luteinized cells, and theca and stroma. The mean level of AMH was four times higher in GC-CM from ovulatory PCOs [mean (range) 1.56 (0.025-7)] and 75 times higher from anovPCO [21.4 (17.2-43 ng/ml)] than normal ovaries [0.37 (0.025-1.7)]. Neither LH nor FSH had an effect on AMH production by GCs from normal ovaries, but in cells from PCOs, FSH significantly decreased AMH, and in contrast, LH increased AMH.
  
  
  CONCLUSIONS
  The reduction of AMH in follicles greater than 9 mm from normal ovaries appears to be an important requirement for the selection of the dominant follicle. AMH production per GC was 75 times higher in anovPCOs, compared with normal ovaries. This increase in AMH may contribute to failure of follicle growth and ovulation seen in polycystic ovary syndrome.
authors.affiliation: Clinical Development Sciences, St. George's University of London, Cranmer Terrace, London SW17 0RE, United Kingdom.
authors.forename: Laura
authors.initials: L
authors.lastname: Pellatt
authors.forename: Laurine
authors.initials: L
authors.lastname: Hanna
authors.forename: Mark
authors.initials: M
authors.lastname: Brincat
authors.forename: Ray
authors.initials: R
authors.lastname: Galea
authors.forename: Henrietta
authors.initials: H
authors.lastname: Brain
authors.forename: Saffron
authors.initials: S
authors.lastname: Whitehead
authors.forename: Helen
authors.initials: H
authors.lastname: Mason
mesh_terms: D000328:Adult; D054304:Anti-Mullerian Hormone; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D015571:Follicular Fluid / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis*; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D007986:Luteinizing Hormone / Q000494:pharmacology; D008875:Middle Aged; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D013735:Testicular Hormones / Q000096:biosynthesis*; D013799:Theca Cells / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D013735:Testicular Hormones; D054304:Anti-Mullerian Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone