title: Application of N-PLS calibration to the simultaneous determination of Cu(2+), Cd(2+) and Pb(2+) using peptide modified electrochemical sensors.
abstract: The simultaneous determination of Cu(2+), Cd(2+) and Pb(2+) is demonstrated at four modified gold electrodes using N-PLS calibration. Three of the electrodes were modified with the peptides Gly-Gly-His, gamma-Glu-Cys Gly and human angiotensin I which were covalently attached to thioctic acid self-assembled monolayers and the fourth electrode was modified with thioctic acid only. Voltammetry at the modified electrodes in the presence of the three metal ions revealed one peak due to the reduction of copper and another due to the overlapping peaks of cadmium and lead which made quantification using conventional methods difficult. N-PLS was used to calibrate and predict trace concentrations (100 nM to 10 microM) of mixtures of Cu(2+), Cd(2+) and Pb(2+).
authors.affiliation: School of Chemistry, The University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Edith
authors.initials: E
authors.lastname: Chow
authors.forename: Diako
authors.initials: D
authors.lastname: Ebrahimi
authors.forename: J Justin
authors.initials: JJ
authors.lastname: Gooding
authors.forename: D Brynn
authors.initials: DB
authors.lastname: Hibbert
mesh_terms: D015374:Biosensing Techniques; D002104:Cadmium / Q000032:analysis; D002138:Calibration; D003300:Copper / Q000032:analysis; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D006046:Gold; D006801:Humans; D007854:Lead / Q000032:analysis; D016018:Least-Squares Analysis; D019216:Metals, Heavy / Q000032:analysis*; D010455:Peptides
chemical_list: D019216:Metals, Heavy; D010455:Peptides; D002104:Cadmium; D007854:Lead; D006046:Gold; D003300:Copper