Region of rat chromosome 8 determines complex nutrigenetic interactions under conditions of sucrose and cholesterol diets.
We have previously established a congenic strain SHR-Lx that carries a differential segment of rat chromosome 8 introgressed from a model of metabolic syndrome--the polydactylous rat strain--on the genomic background of spontaneously hypertensive rat (SHR). We compared the glucose tolerance and lipid profile of adult SHR and SHR-Lx males under conditions of standard diet and diets enriched in sucrose and cholesterol, respectively. While there was no evident difference between the SHR and SHR-Lx on standard diet, the one-week sucrose administration revealed the congenic strain sensitivity to carbohydrate-induced dyslipidemia conferred by the differential segment with only mild derangement of glucose tolerance. On the other hand, the high-cholesterol diet administration for three-weeks resulted in a contrasting pattern as the congenic strain displayed significantly lower concentrations of free fatty acids and improved glucose tolerance compared to SHR. After one-month washout period, the SHR-Lx showed higher insulin, triglyceride and cholesterol concentrations together with diminished insulin sensitivity of visceral adipose tissue. In summary, we have identified a genomic region syntenic to human chromosome 11q23, which determines complex nutrigenetic interactions under conditions of sucrose- and cholesterol-enriched diets.
Seda
O
O
Institute of Biology and Medical Genetics of the First Faculty of Medicine, Charles University in Prague, Albertov 4, 128 00 Prague 2, Czech Republic. oseda@lf1.cuni.cz
Kazdová
L
L
Sedová
L
L
Liska
F
F
Krenová
D
D
Kren
V
V
D000824:Animal Nutritional Physiological Phenomena / Q000235:genetics*; D000818:Animals; D020296:Animals, Congenic; D001786:Blood Glucose / Q000032:analysis; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D033481:Chromosomes, Mammalian; D032461:Chromosomes, Plant; D019422:Dietary Sucrose / Q000008:administration & dosage*; D005951:Glucose Tolerance Test; D006003:Glycogen / Q000096:biosynthesis; D007328:Insulin / Q000494:pharmacology; D050356:Lipid Metabolism / Q000235:genetics; D050155:Lipogenesis / Q000187:drug effects; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR
D001786:Blood Glucose; D002791:Cholesterol, Dietary; D019422:Dietary Sucrose; D007328:Insulin; D006003:Glycogen