title: Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts.
abstract: Hepatic stellate cells (HSC) and liver myofibroblasts (MFB) are two cell populations most likely responsible for the synthesis of most connective tissue components in fibrotic liver. They differ in their origin and location, and possibly in patterns of gene expression. Normal and carbon tetrachloride-cirrhotic livers from rats were used to isolate HSC. Liver was perfused with pronase and collagenase solutions, followed by centrifugation of the cell suspension on a density gradient. HSC were quiescent 2 days after plating on plastic but they became activated after another 5 days in culture. When the culture was passaged 5 times, its character changed profoundly as HSC were replaced by MFB. Microarray analysis was used to determine gene expression in quiescent HSC, activated HSC and MFB. The expression of 49 genes coding for connective tissue proteins, proteoglycans, metalloproteinases and their inhibitors, growth factors and cellular markers was determined. The pattern of gene expression changed during HSC activation and there were distinct differences between HSC and MFB. Little difference between normal cells and cells isolated from cirrhotic liver was found.
authors.affiliation: Charles University in Prague, Faculty of Medicine in Hradec Kralove, Department of Medical Biochemistry, Simkova 870, 500 38 Hradec Kralove, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Jiroutova
authors.forename: Rastislav
authors.initials: R
authors.lastname: Slavkovsky
authors.forename: Martina
authors.initials: M
authors.lastname: Cermakova
authors.forename: Lenka
authors.initials: L
authors.lastname: Majdiakova
authors.forename: Irena
authors.initials: I
authors.lastname: Hanovcova
authors.forename: Radka
authors.initials: R
authors.lastname: Bolehovska
authors.forename: Milena
authors.initials: M
authors.lastname: Hajzlerova
authors.forename: Hana
authors.initials: H
authors.lastname: Radilova
authors.forename: Ema
authors.initials: E
authors.lastname: Ruszova
authors.forename: Jiri
authors.initials: J
authors.lastname: Kanta
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D002478:Cells, Cultured; D003238:Connective Tissue / Q000187:drug effects / Q000378:metabolism*; D005109:Extracellular Matrix / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism; D045726:Metalloproteases / Q000235:genetics / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000187:drug effects; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism
chemical_list: D011509:Proteoglycans; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D002251:Carbon Tetrachloride; D045726:Metalloproteases