A method for cold storage and transport of viable embryonic kidney rudiments.
Organ culture of mouse embryonic kidneys is a powerful system for studying normal renal development and for researching the developmental effects of experimental perturbations (drugs, antibodies, interfering RNA, and so on). In standard protocols, embryonic kidneys are isolated by delicate micro-dissection and placed in organ culture as soon as possible after the death of the donor mouse, before there is time to genotype them or to transport them elsewhere. This report demonstrates that fully viable embryonic kidneys can be isolated and cultured from crudely dissected caudal portions of embryos that have been stored on ice or at 4 degrees C for several days. This very simple technique can save considerable resources in laboratories that culture kidneys of transgenic mice: (i) cold storage allows embryos to be genotyped before their kidneys are cultured, and (ii) cold transport allows kidney research laboratories to study kidneys of transgenic mice raised elsewhere without the need for expensive importing of the mouse line itself. It will therefore substantially augment the ability of kidney research labs to perform pilot experiments on large numbers of different transgenic animals and to collaborate in new ways.
Davies
J A
JA
Centre for Integrative Physiology, University of Edinburgh, George Square, Edinburgh, UK. Jamie.Davies@ed.ac.uk
D000818:Animals; D003080:Cold Temperature; D004622:Embryo, Mammalian; D007668:Kidney; D051379:Mice; D009924:Organ Culture Techniques; D009926:Organ Preservation / Q000379:methods*