The soluble form of human leukocyte antigen class I antigen causes apoptosis on hepatocellular carcinoma cell lines.
A soluble form of human leukocyte antigen class I antigen (sHLA-I) has been reported to cause apoptosis on cytotoxic T cells and inhibit killer activity of natural killer cells via killer-cell inhibitory receptors. However, its effect on cancer cells has not yet been elucidated. We examined the direct effect of sHLA-I on human liver cancer cell lines, HepG2, HLE and HLF. The effects of sHLA-I on cell growth, DNA synthesis, and apoptosis induction were evaluated. To elucidate the mechanisms, cDNA expression arrays were also examined. sHLA-I caused cell growth inhibition, resulting in apoptosis on human hepatocellular carcinoma, dose-dependently. In this process, caspase-3 was activated. sHLA-I also inhibited in vivo growth of hepatocellular carcinoma in severe combined immunodeficient mice. sHLA-I caused apoptosis on human hepatocellular carcinoma.
Shimura
Tatsuo
T
Department of General Surgical Science (Surgery I), Gunma University Graduate School of Medicine, Gunma 371-8511, Japan. tshimura@showa.gunma-u.ac.jp
Suehiro
Taketoshi
T
Suzuki
Hideki
H
Okada
Koji
K
Araki
Kenichiro
K
Asao
Takayuki
T
Kuwano
Hiroyuki
H
D017398:Alternative Splicing; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D015395:Histocompatibility Antigens Class I / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008113:Liver Neoplasms / Q000473:pathology*; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D020411:Oligonucleotide Array Sequence Analysis; D020033:Protein Isoforms / Q000302:isolation & purification / Q000494:pharmacology
D000970:Antineoplastic Agents; D015395:Histocompatibility Antigens Class I; D020033:Protein Isoforms