title: Inherited thrombophilias and pre-eclampsia in Brazilian women.
abstract: OBJECTIVE
  The aim of the present study was to compare the distribution of G1691A, G20210A and C677T mutations in pre-eclamptic Brazilian women and in matched control women with an uncomplicated normal pregnancy.
  
  
  METHODS
  these mutations were investigated by PCR-RFLP in 83 normal pregnancies (control group) and in 30 pre-eclamptic pregnant women (severe form).
  
  
  RESULTS
  G1691A mutation was detected neither in the control group nor in pre-eclamsia women. G20210A mutation was detected in heterozygosis in 3 (3.61%) control subjects, but not in pre-eclampsia group. C677T mutation was detected in homozygosis in 6 (7.23%) control subjects and 2 (6.67%) pre-eclamptic women and in heterozygosis in 31 (37.3%) control subjects and 12 (40%) pre-eclamptic women. Differences in the mutation frequencies detected in the two groups were not statistically significant.
  
  
  CONCLUSIONS
  No correlation was observed between pre-eclampsia and presence of G1691A, G20210A and C677T mutations in Brazilian women.
authors.affiliation: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. lucim@farmacia.ufmg.br
authors.forename: Luci Maria Santana
authors.initials: LM
authors.lastname: Dusse
authors.forename: Maria das Graças
authors.initials: Md
authors.lastname: Carvalho
authors.forename: William Fialho
authors.initials: WF
authors.lastname: Bragança
authors.forename: Sabrina Guimarães
authors.initials: SG
authors.lastname: Paiva
authors.forename: Lara Carvalho
authors.initials: LC
authors.lastname: Godoi
authors.forename: Daniela Amorim Melgaço
authors.initials: DA
authors.lastname: Guimarães
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Fernandes
mesh_terms: D001938:Brazil; D016022:Case-Control Studies; D005165:Factor V / Q000235:genetics*; D005260:Female; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D011516:Prothrombin / Q000235:genetics*; D019851:Thrombophilia / Q000235:genetics
chemical_list: C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin; D042965:Methylenetetrahydrofolate Reductase (NADPH2)