title: Preimplantation and fetal development of mouse embryos cultured in a protein-free, chemically defined medium.
abstract: Mouse two-cell embryos of block (ICR) or nonblock (F1 of C57BL x DBA) strains were cultured in modified Chatot, Ziomek, and Bavister medium, supplemented with bovine serum albumin or polyvinyl alcohol, a synthetic macromolecule. The supplementation did not influence morula compaction and blastocyst formation, mean cell numbers of total blastomeres, inner cell mass (ICM) cells and trophectodermal (TE) cells in blastocysts, rates of pregnancy and delivery, mean litter size, number of implantation spots in pregnant females, and the expression of several genes related to pluripotency, organogenesis, and implantation.
authors.affiliation: Department of Food and Animal Biotechnology, Seoul National University, Seoul, South Korea.
authors.forename: Mi
authors.initials: M
authors.lastname: Jang
authors.forename: Eun J
authors.initials: EJ
authors.lastname: Lee
authors.forename: Seung Tae
authors.initials: ST
authors.lastname: Lee
authors.forename: Meeyoung
authors.initials: M
authors.lastname: Cho
authors.forename: Jeong M
authors.initials: JM
authors.lastname: Lim
mesh_terms: D000818:Animals; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology*; D005240:Feasibility Studies; D005260:Female; D047109:Fetal Development / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D009924:Organ Culture Techniques / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D003470:Culture Media; D011506:Proteins