[Changes of retinal ganglion cells and expression of Bad after optic nerve crush in rats].
OBJECTIVE
To observe the change of retinal ganglion cells (RGCs)and the expression of Bad after optic nerve injury, so as to study the changes of optic function level on morphology and molecular.


METHODS
The experimental models of optic nerve crush were established in fifty Wistar rats. At the different time after injuries (from one to twenty-eight day), the changes of RGCs were observed under microscope. Immunohistochemiscal technique and computer image analysis methods were performed to observe the changes of Bad in RGCs in rats.


RESULTS
The number of RGCs was reduced significantly according to partial lesion of optic nerve crush. An initial loss of RGCs densities was accelerated in one week after nerve crush, two weeks later the trend mitigated. After four weeks, no obvious change were observed. The expression of Bad increased in 3 days, reached peak in 5 days, and declined one week later. No obvious changes were observed after two weeks.


CONCLUSIONS
The expression of Bad lead to the loss of RGCs following optic nerve crush. This is the important reason of loss optic function. The identification on optic nerve injuries should be done at least four weeks later.
Wu
Hong-Se
HS
Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
Ke
Ji
J
Chen
Xiao-Rui
XR
D000818:Animals; D016923:Cell Death; D004195:Disease Models, Animal; D005260:Female; D005554:Forensic Medicine; D008297:Male; D009409:Nerve Crush; D009900:Optic Nerve / Q000473:pathology / Q000503:physiopathology; D020221:Optic Nerve Injuries / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012165:Retinal Ganglion Cells / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D051025:bcl-Associated Death Protein / Q000378:metabolism*
C494821:Bad protein, rat; D051025:bcl-Associated Death Protein