A bio-barcode assay for on-chip attomolar-sensitivity protein detection.
Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticles--a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of prostate specific antigen (PSA) in buffer solution and goat serum. Detection was accomplished at PSA concentrations as low as 500 aM. This corresponds to only 300 copies of protein analytes using 1 microL total sample volume. We established that the on-chip BCA for PSA detection offers four orders of magnitude higher sensitivity compared to commercially available ELISA-based PSA tests.
Goluch
Edgar D
ED
Micro and Nanotechnology Laboratory, Bioengineering Department, University of Illinois at Urbana-Champaign, 208 N. Wright Street, Urbana, IL 61801, USA.
Nam
Jwa-Min
JM
Georganopoulou
Dimitra G
DG
Chiesl
Thomas N
TN
Shaikh
Kashan A
KA
Ryu
Kee S
KS
Barron
Annelise E
AE
Mirkin
Chad A
CA
Liu
Chang
C
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats; D046210:Microfluidic Analytical Techniques; D053758:Nanoparticles / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000097:blood*; D012680:Sensitivity and Specificity
D017430:Prostate-Specific Antigen