Reagentless ultrasensitive specific DNA array detection based on responsive polymeric biochips.
Self-assembled molecular structures immobilized on solid substrates and composed of fluorophore-tagged oligonucleotide probes and an optical polymeric transducer were investigated for the trace level detection of DNA target molecules. Rapid and efficient energy transfer between the polymeric transducer and fluorophores within the molecular aggregates leads to a massive intrinsic amplification of the fluorescence signal and to the label-free detection of as little as 300 DNA molecules, with the specificity required for the detection of single-nucleotide mismatches. This capacity for attomolar detection levels while the sensing structures are attached onto solid supports could lead to the development of biochip platforms for fast and simple PCR-free multitarget DNA detection.
Najari
Ahmed
A
Department of Chemistry and Centre de Recherches sur la Science et L'ingénierie des Macromolécules (CERSIM), Université Laval, Québec, Canada G1K 7P4.
Ho
Hoang Anh
HA
Gravel
Jean-François
JF
Nobert
Philippe
P
Boudreau
Denis
D
Leclerc
Mario
M
D020137:Base Pair Mismatch / Q000235:genetics; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000032:analysis* / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D004247:DNA