Fluorescence preselection of bioaerosol for single-particle mass spectrometry.
We have designed, constructed, and tested a system that pre-selects the biological fraction of airborne particles from the overall aerosol. The preselection is based on fluorescence emission excited by a continuous 266 nm laser beam. This beam is one of two cw beams used to measure the aerodynamic particle size of sampled particles. The intention in our system is that single particles, based on size and fluorescence emission, can be selected and further examined for chemical composition by mass spectrometry.
Stowers
M A
MA
Faculty of Applied Sciences, Delft University of Technology, Julianalaan 136, 2628 BL Delft, The Netherlands. m.a.stowers@tnw.tudelft.nl
van Wuijckhuijse
A L
AL
Marijnissen
J C M
JC
Kientz
Ch E
ChE
Ciach
T
T
D000336:Aerosols / Q000032:analysis*; D000393:Air Pollutants / Q000032:analysis*; D001704:Biopolymers / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013171:Spores, Bacterial / Q000302:isolation & purification*
D000336:Aerosols; D000393:Air Pollutants; D001704:Biopolymers