title: Effects of vaporized perfluorohexane and partial liquid ventilation on regional distribution of alveolar damage in experimental lung injury.
abstract: OBJECTIVE
  To determine whether the patterns of distribution of histological effects of vaporized perfluorohexane (PFH) and partial liquid ventilation (PLV) differ significantly in acute lung injury.
  
  
  METHODS
  Experimental study in an animal research laboratory.
  
  
  METHODS
  Eighteen pigs.
  
  
  METHODS
  After induction of acute lung injury by means of infusion of oleic acid animals were randomly assigned to PFH, PLV, or gas ventilation (GV) groups. Six hours thereafter animals were killed, and lung tissue samples were taken for analysis.
  
  
  RESULTS
  Histopathological analysis revealed less damage with PFH than with GV or PLV in the nondependent and central regions. PFH and PLV showed less injury in the dependent regions than GV. GV and PFH were associated with less histological damage in the nondependent than the dependent regions, whereas PLV presented the opposite pattern. Morphometric analysis showed increased aeration in nondependent than dependent regions with PFH and GV. PLV led to more aeration in the periphery than in central areas.
  
  
  CONCLUSIONS
  PFH was associated with a more homogeneous attenuation of alveolar damage across the lungs, although this therapy had more pronounced effects in nondependent zones. PLV showed the opposite pattern, with more important reduction in alveolar damage in dependent lung regions. Interestingly, reduction in alveolar damage with PFH was as effective as with PLV in dependent zones. Our findings suggest that vaporized perfluorocarbon could be advantageous as adjunctive therapy in the treatment of acute lung injury.
authors.affiliation: Clinic for Anesthesiology and Intensive Care Therapy, Carl Gustav Carus Medical College, Technical University of Dresden and University Hospital, Fetscherstrasse 74, 01307, Dresden, Germany. p.spieth@web.de
authors.forename: Peter M
authors.initials: PM
authors.lastname: Spieth
authors.forename: Lilla
authors.initials: L
authors.lastname: Knels
authors.forename: Michael
authors.initials: M
authors.lastname: Kasper
authors.forename: André
authors.initials: A
authors.lastname: Domingues Quelhas
authors.forename: Bärbel
authors.initials: B
authors.lastname: Wiedemann
authors.forename: Amelie
authors.initials: A
authors.lastname: Lupp
authors.forename: Matthias
authors.initials: M
authors.lastname: Hübler
authors.forename: Antonio Giannella
authors.initials: AG
authors.lastname: Neto
authors.forename: Antonio
authors.initials: A
authors.lastname: Gianella Neto
authors.forename: Thea
authors.initials: T
authors.lastname: Koch
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gama de Abreu
mesh_terms: D000818:Animals; D005466:Fluorocarbons / Q000627:therapeutic use*; D021061:Liquid Ventilation / Q000379:methods*; D011175:Positive-Pressure Respiration; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000473:pathology*; D012128:Respiratory Distress Syndrome / Q000188:drug therapy* / Q000473:pathology / Q000628:therapy; D013552:Swine; D014835:Volatilization
chemical_list: D005466:Fluorocarbons; C078626:perflexane