A PCR-based method for detection and quantification of small RNAs.
Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method.
Ro
Seungil
S
Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA.
Park
Chanjae
C
Jin
Jingling
J
Sanders
Kenton M
KM
Yan
Wei
W
D000818:Animals; D001483:Base Sequence; D020869:Gene Expression Profiling / Q000379:methods*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods*; D014018:Tissue Distribution
D035683:MicroRNAs