Nesterenkonia jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood.
A Gram-positive, non-motile, slightly halophilic actinomycete, strain JG-241T, was isolated from jeotgal, a traditional Korean fermented seafood, and its taxonomic position was investigated by using a polyphasic approach. Strain JG-241T grew optimally at 25-30 degrees C and in the presence of 2-5% (w/v) NaCl. The physiological and biochemical properties of strain JG-241T were distinguishable from those of recognized Nesterenkonia species. Strain JG-241T had a peptidoglycan type based on L-lys-gly-D-Asp. It contained MK-7, MK-8 and MK-9 as the predominant menaquinones and anteiso-C15:0) and anteiso-C17:0 as the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid. The DNA G+C content was 68.0 mol%. Comparative 16S rRNA gene sequence analysis showed that strain JG-241T falls within the radiation of the cluster comprising Nesterenkonia species. Similarity values between the 16S rRNA gene sequence of strain JG-241T and those of the type strains of Nesterenkonia species ranged from 96.7 to 99.7%. DNA-DNA relatedness data and repetitive extragenic palindromic DNA-PCR genomic fingerprinting patterns showed that strain JG-241T differs genetically from recognized Nesterenkonia species. On the basis of its phenotypic properties and phylogenetic and genetic distinctiveness, strain JG-241T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia jeotgali sp. nov. is proposed. The type strain is JG-241T (=KCTC 19053T=JCM 12610T).
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Jung
Seo-Youn
SY
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Kim
Wonyong
W
Department of Microbiology, College of Medicine, Chungang University, 221 Heukseok-dong, Seoul, Korea.
Nam
Sun-Woo
SW
Health Technology Planning and Evaluation Board, 57-1 Noryangjin-dong, Seoul, Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
D001482:Base Composition; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D005516:Food Microbiology; D020459:Genes, rRNA; D006131:Growth Inhibitors / Q000494:pharmacology; D007723:Korea; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D008835:Micrococcaceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D011809:Quinones / Q000032:analysis / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006131:Growth Inhibitors; D008055:Lipids; D010457:Peptidoglycan; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride