title: Antigens recognized by IgG derived from tumor-infiltrating B lymphocytes in human lung cancer.
abstract: BACKGROUND
  Lung cancer tissues are often infiltrated by B lymphocytes, but it is not clear whether these infiltrations represent tumor-specific immune response or a nonspecific reaction.
  
  
  METHODS
  The serological analysis of recombinant cDNA expression libraries (SEREX) were previously modified using a severe combined immunodeficient (SCID) mice model engrafted with fresh human lung cancer. Here, a panel of antigens recognized by tumor-infiltrating B lymphocytes (TIB) in human lung cancer were characterized.
  
  
  RESULTS
  The modified SEREX analysis identified 22 distinct antigens in a large cell carcinoma of the lung. Sequence analysis and real time-PCR analysis showed that 55% of isolated antigens were overexpressed in tumor tissues and 9% had mutation.
  
  
  CONCLUSIONS
  The results of this study indicate that the humoral immune response of TIB in lung cancer patients can be detected in the xenotransplanted SCID mouse model and our modification shows high sensitivity and specificity for identification of tumor antigens.
authors.affiliation: Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan. m-yasuda@med.uoeh-u.ac.jp
authors.forename: Manabu
authors.initials: M
authors.lastname: Yasuda
authors.forename: Makiko
authors.initials: M
authors.lastname: Mizukami
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hanagiri
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Shigematsu
authors.forename: Takashi
authors.initials: T
authors.lastname: Fukuyama
authors.forename: Yoshika
authors.initials: Y
authors.lastname: Nagata
authors.forename: Tetsuya
authors.initials: T
authors.lastname: So
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ichiki
authors.forename: Masakazu
authors.initials: M
authors.lastname: Sugaya
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Takenoyama
authors.forename: Kenji
authors.initials: K
authors.lastname: Sugio
authors.forename: Kosei
authors.initials: K
authors.lastname: Yasumoto
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018287:Carcinoma, Large Cell / Q000276:immunology / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000378:metabolism; D008175:Lung Neoplasms / Q000276:immunology* / Q000378:metabolism; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D014183:Transplantation, Heterologous
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D007074:Immunoglobulin G