[NEMOes, a new method to evaluate early atherosclerosis in rodent model].
We recently established new en face method for optimal observation of endothelial surface(NEMOes) that enabled us to obtain clear images of the entire endothelial surface, including at arterial bifurcations, and to quantitate the number of cells of interest in the rodent endothelium. Using this method, we found that not only advanced glycosylation endproducts, but also glucose spike induced a significant increase in the number of macrophages adhering to the endothelium. NEMOes may be a promising tool for understanding the pathophysiology of atherosclerosis.
Watada
Hirotaka
H
Department of Medicine, Metabolism & Endocrinology, Juntendo University School of Medicine.
Kawamori
Ryuzo
R
D000818:Animals; D050197:Atherosclerosis / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology* / Q000517:prevention & control; D002448:Cell Adhesion; D002452:Cell Count; D003925:Diabetic Angiopathies / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology* / Q000517:prevention & control; D004195:Disease Models, Animal; D042241:Early Diagnosis; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D008264:Macrophages / Q000502:physiology; D051381:Rats
D017127:Glycation End Products, Advanced