title: [Mechanism of peptide bond formation on the ribosome--controversions].
abstract: During past five years there have been published many experimental data concerning structure and function of the ribosome. With the presentation of atomic structures we obtained a new data about composition of peptidyl transferase center. It is now obvious that PTC is composed entirely of rRNA. It is also known that the proper substrate alignment is the major factor for ribosome's catalytic activity. However, more detailed mechanism of peptide bond formation on the ribosome still remains unclear. Several issues remain unsolved. For example, are there any chemical components coming from ribosome itself, that enhance the rate of the reaction? Do intact ribosomes perform peptidyltransfer in the same way as the isolated ribosomal subunits that have been the source of most of the data? In this article we present different opinions and controversions around peptide bond formation on the ribosome.
authors.affiliation: Instytut Chemii Bioorganicznej PAN, Poznań.
authors.forename: Kamilla
authors.initials: K
authors.lastname: Bakowska-Zywicka
authors.forename: Agata
authors.initials: A
authors.lastname: Tyczewska
authors.forename: Tomasz
authors.initials: T
authors.lastname: Twardowski
mesh_terms: D002384:Catalysis; D018360:Crystallography, X-Ray; D019614:Haloarcula marismortui / Q000378:metabolism; D007700:Kinetics; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010452:Peptide Biosynthesis / Q000502:physiology*; D010458:Peptidyl Transferases / Q000737:chemistry* / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D012345:RNA, Transfer, Amino Acid-Specific / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D012335:RNA, Ribosomal; D012345:RNA, Transfer, Amino Acid-Specific; D010458:Peptidyl Transferases