Isolation of protoplast from aerial mycelia and fruit-body formation from regenerated mycelia in Pleurotus cornucopiae.
We have developed a simple and efficient method for protoplast isolation from Pleurotus cornucopiae. Protoplasts were isolated from aerial mycelia cultured on potato dextrose agar medium without time-consuming propagation in liquid culture. Protoplast yield was significantly increased by means of a decompressing pretreatment of mycelia in enzyme solution and a subsequent enzyme reaction with vibrational mixing. The isolated protoplasts regenerated mycelia and these mycelia formed fruit-bodies without any morphological abnormalities.
Mizoguchi
Masahide
M
Department of Biology, Faculty of Science and Engineering, Konan University, Okamoto, Higashinada-ku, Kobe, Japan.
Kitano
Satoshi
S
Takahashi
Wataru
W
Sugawara
Fuyuki
F
Tanaka
Osamu
O
D020076:Pleurotus / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism*; D014732:Vibration