Transcriptional regulator Id2 mediates CD8+ T cell immunity.
Transcriptional programs that initiate and sustain the proliferation, differentiation and survival of CD8(+) T cells during immune responses are not completely understood. Here we show that inhibitor of DNA binding 2 (Id2), an antagonist of E protein transcription factors, was upregulated in CD8(+) T cells during infection and that expression of Id2 was maintained in memory CD8(+) T cells. Although Id2-deficient naive CD8(+) T cells recognized antigen and proliferated normally early after infection, effector CD8(+) T cells did not accumulate because the cells were highly susceptible to apoptosis. Id2-deficient CD8(+) T cells responding to infection had changes in the expression of genes that influence survival and had altered memory formation. Our data emphasize the importance of Id2 in regulating gene expression by CD8(+) T cells and the magnitude of effector responses, suggesting a mechanism involving Id protein- and E protein-mediated survival and differentiation of mature T cells.
Cannarile
Michael A
MA
Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093, USA.
Lind
Nicholas A
NA
Rivera
Richard
R
Sheridan
Alison D
AD
Camfield
Kristin A
KA
Wu
Bei Bei
BB
Cheung
Kitty P
KP
Ding
Zhaoqing
Z
Goldrath
Ananda W
AW
D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D015870:Gene Expression / Q000276:immunology; D007156:Immunologic Memory; D051797:Inhibitor of Differentiation Protein 2 / Q000276:immunology* / Q000378:metabolism; D008088:Listeriosis / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D014158:Transcription, Genetic
C497452:Idb2 protein, mouse; D051797:Inhibitor of Differentiation Protein 2