[Expression of DPP4 gene in papillary thyroid carcinoma].
BACKGROUND
DPP4 gene (dipeptidyl peptidase IV) is expressed in epithelial cells of many organs and cells of immune system. There is no expression of DPP4 in normal healthy thyroid, while it is highly expressed in papillary thyroid carcinoma (PTC), as shown by gene expression profiling. In this study we validated expression of DPP4 in papillary thyroid cancer and normal thyroid tissue and evaluated its usefulness for diagnostic purposes.


METHODS
The analysis was carried out on total RNA extracted from 102 PTCs and 77 normal thyroid fragments with use of Q-PCR reaction. Beta-glucuronidase was the reference gene.


RESULTS
We confirmed the distinct increase of DPP4 expression in papillary thyroid carcinoma. However, the ROC (relative operating characteristic) analysis revealed that the diagnostic efficiency of DPP4 estimation is limited.


CONCLUSIONS
DPP4 is increased in papillary thyroid cancer, however, its diagnostic usefulness as a single PTC marker is doubtful.
Ozóg
Joanna
J
Department of Nuclear Medicine and Endocrine Oncology, Comprehensive Cancer Center and Institute of Oncology-M. Skłodowskiej-Curie Memorial Institute, Gliwice Branch, ul. Wybrzeże Armii Krajowej 15, Gliwice.
Jarzab
Michał
M
Pawlaczek
Agnieszka
A
Oczko-Wojciechowska
Małgorzata
M
Włoch
Jan
J
Roskosz
Józef
J
Gubała
Elzbieta
E
D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002277:Carcinoma; D002291:Carcinoma, Papillary; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D018819:Dipeptidyl Peptidase 4 / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000235:genetics*
D014408:Biomarkers, Tumor; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4