Characteristics of frozen-thawed spermatozoa cryopreserved with different concentrations of glycerol in captive Japanese black bears (Ursus thibetanus japonicus).
Seven mature Japanese black bears were used as semen donors, and a total of 7 semen samples collected from the animals by the electroejaculation method were cryopreserved in liquid nitrogen. Egg yolk-TRIS-citrate-glucose extender was used, and the effects of different final concentrations of glycerol, at 4-12% (v/v), on frozen-thawed spermatozoa were examined. No significant difference was observed in percent motility or percent abnormal morphology of frozen-thawed spermatozoa among the different glycerol concentrations. Percent viability and percent intact acrosomes of spermatozoa cryopreserved with 4 and 6% glycerol were significantly higher than those with 10 and 12% glycerol. These results suggest that a suitable glycerol concentration for freezing Japanese black bear semen within the range tested would be 4-6%.
Okano
Tsukasa
T
United Graduate School of Veterinary Sciences, Gifu University, Japan.
Nakamura
Sachiko
S
Komatsu
Takeshi
T
Murase
Tetsuma
T
Miyazawa
Kiyoshi
K
Asano
Makoto
M
Tsubota
Toshio
T
D000177:Acrosome / Q000502:physiology; D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005990:Glycerol; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D001503:Ursidae / Q000502:physiology*
D005990:Glycerol