Structure and serological analysis of the Hafnia alvei 481-L O-specific polysaccharide containing phosphate in the backbone chain.
The lipopolysaccharide was extracted from cells of Hafnia alvei 481-L bacterial strain and, after mild acid hydrolysis, the O-specific polysaccharide was isolated and characterised. On the basis of chemical analyses and NMR spectroscopic studies of the polysaccharide and oligosaccharides obtained after Smith degradation, or hydrogen fluoride treatment, it was found that the repeating unit of the O-specific polysaccharide is a phosphorylated hexasaccharide: [see text]. The biological repeating unit of the H. alvei 481-L O-antigen has galactose phosphate at the nonreducing terminus. Serological tests indicate that this strain represents an individual serotype in the H. alvei genus.
Kübler-Kiełb
J
J
L. Hirszfeld Institute of Immunology and Experimental, Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
Vinogradov
E
E
García Fernández
J M
JM
Szostko
B
B
Zwiefka
A
A
Gamian
A
A
D000818:Animals; D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D020611:Hafnia alvei / Q000737:chemistry; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology*; D011817:Rabbits; D012698:Serologic Tests; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013403:Sugar Phosphates / Q000737:chemistry*
C430211:O-specific polysaccharide, Hafnia alvei; D011135:Polysaccharides, Bacterial; D013403:Sugar Phosphates