Colloidal gold-based immunochromatographic assay for detection of diethylstilbestrol residues.
One-step membrane-based competitive colloidal gold-based immunoassays in immunochromatographic formats for the rapid detection of diethylstilbestrol (DES) were developed. Nitro-cellulose membrane strip was separately coated with goat anti-rabbit IgG (control line) and DES hapten-ovalubumin conjugate (test line). Anti-DES polyclonal antibody labeled with colloidal gold particles was first incubated with DES. A positive reaction as a result of the remaining antibody-gold conjugate combining with antigen coated on the membrane was obvious by visual detection, with detection limits for immunochromatographic of 0.5 microg/kg for detecting DES standard solution, and the limit of detection was 5 microg/kg for detecting the DES spiked in swine pork and liver. The assay time for test was less than 5 min, suitable for rapid testing on-site.
Chuanlai
Xu
X
School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, People's Republic of China. XCL@sytu.edu.cn
Huting
Wang
W
Chifang
Peng
P
Zhengyu
Jin
J
Liqiang
Liu
L
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004054:Diethylstilbestrol / Q000032:analysis* / Q000276:immunology; D017979:Gold Colloid; D007118:Immunoassay / Q000379:methods*; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods; D013552:Swine
D017979:Gold Colloid; D004054:Diethylstilbestrol