Naturally occurring resistance of bone marrow mononuclear and metastatic cancer cells to anticancer agents.
Numerous cancer patients fail standard chemotherapy or develop resistance to chemotherapy during the course of treatment. The purpose of this study is to elucidate the overall response of cells obtained from cancer patients and from normal individuals to chemotherapeutic agents. We analysed the chemosensitivity of cancer cells derived from bone marrow and from pleural effusions or ascites fluids from patients with different cancers. Chemosensitivity to doxorubicin, cisplatin and paclitaxel was determined using the MTT assay. We also determined the response of bone marrow mononuclear (BMMN) cells. There was a wide range of responses to chemotherapy drugs in samples from different individuals. This was observed in cells derived from bone marrow and from ascites or pleural fluids. Large variations were also observed among morphologically normal BMMN cells and metastatic cancer cells from chemo-naïve patients. Cancer cells can easily be collected from ascites or pleural fluids and reliably assayed for chemosensitivity. We describe here that inherent chemoresistance may be a reason for the lack of response to chemotherapy in some patients. We discuss the potential of using the determination of natural resistance to dictate the drugs to be employed for treatment.
Richard
Christina
C
Regional Cancer Care Programme, Thunder Bay Regional Health Sciences Centre, 980 Oliver Road, Thunder Bay, ON, Canada.
Yau
Jonathan
J
Th'ng
John P H
JP
Duivenvoorden
Wilhelmina C M
WC
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001202:Ascitic Fluid / Q000166:cytology; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology; D002470:Cell Survival; D002945:Cisplatin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D005260:Female; D005562:Formazans / Q000032:analysis; D006801:Humans; D020128:Inhibitory Concentration 50; D008297:Male; D009362:Neoplasm Metastasis / Q000473:pathology*; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000473:pathology; D017239:Paclitaxel / Q000494:pharmacology / Q000627:therapeutic use; D016066:Pleural Effusion, Malignant / Q000166:cytology; D013778:Tetrazolium Salts / Q000032:analysis
D000970:Antineoplastic Agents; D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan; D004317:Doxorubicin; D017239:Paclitaxel; D002945:Cisplatin