Comparative study on central metabolic fluxes of Bacillus megaterium strains in continuous culture using 13C labelled substrates.
Fluxes of central carbon metabolism [glycolysis, pentose phosphate pathway (PPP), tricarboxylic acid cycle (TCA cycle), biomass formation] were determined for several Bacillus megaterium strains (DSM319, WH320, WH323, MS941) in C- and N-limited chemostat cultures by (13)C labelling experiments. The labelling patterns of proteinogenic amino acids were analysed by GC/MS and therefrom flux ratios at important nodes within the metabolic network could be calculated. On the basis of a stoichiometric metabolic model flux distributions were estimated for the different B. megaterium strains used at various cultivation conditions. Generally all strains exhibited similar metabolic flux distributions, however, several significant changes were found in (1) the glucose flux entering the PPP via the oxidative branch, (2) the reversibilities within the PPP, (3) the relative fluxes of pyruvate and acetyl-CoA fed to the TCA cycle, (4) the fluxes around the pyruvate node involving a futile cycle.
Fürch
Tobias
T
Institute of Biochemical Engineering (TU-BCE), Technical University Braunschweig/HZI-Helmholtz Zentrum für Infektionsforschung, Inhoffenstrasse 7, 38124 Braunschweig, Germany. Tobias.Fuerch@helmholtz-hzi.de
Hollmann
Rajan
R
Wittmann
Christoph
C
Wang
Wei
W
Deckwer
Wolf-Dieter
WD
D001410:Bacillus megaterium / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D002247:Carbon Isotopes; D002952:Citric Acid Cycle; D007700:Kinetics; D008962:Models, Theoretical; D010427:Pentose Phosphate Pathway; D013045:Species Specificity; D013379:Substrate Specificity
D002247:Carbon Isotopes