In vivo migration of labeled autologous natural killer cells to liver metastases in patients with colon carcinoma.
BACKGROUND
Besides being the effectors of native anti-tumor cytotoxicity, NK cells participate in T-lymphocyte responses by promoting the maturation of dendritic cells (DC). Adherent NK (A-NK) cells constitute a subset of IL-2-stimulated NK cells which show increased expression of integrins and the ability to adhere to solid surface and to migrate, infiltrate, and destroy cancer. A critical issue in therapy of metastatic disease is the optimization of NK cell migration to tumor tissues and their persistence therein. This study compares localization to liver metastases of autologous A-NK cells administered via the systemic (intravenous, i.v.) versus locoregional (intraarterial, i.a.) routes.


METHODS
A-NK cells expanded ex-vivo with IL-2 and labeled with (111)In-oxine were injected i.a. in the liver of three colon carcinoma patients. After 30 days, each patient had a new preparation of (111)In-A-NK cells injected i.v. Migration of these cells to various organs was evaluated by SPET and their differential localization to normal and neoplastic liver was demonstrated after i.v. injection of 99mTc-phytate.


RESULTS
A-NK cells expressed a donor-dependent CD56+ CD16+ CD3- (NK) or CD56+ CD16+ CD3+ (NKT) phenotype. When injected i.v., these cells localized to the lung before being visible in the spleen and liver. By contrast, localization of i.a. injected A-NK cells was virtually confined to the spleen and liver. Binding of A-NK cells to liver neoplastic tissues was observed only after i.a. injections.


CONCLUSIONS
This unique study design demonstrates that A-NK cells adoptively transferred to the liver via the intraarterial route have preferential access and substantial accumulation to the tumor site.
Matera
Lina
L
Dept of Internal Medicine, University of Turin, Italy. lina.matera@unito.it
Galetto
Alessandra
A
Bello
Marilena
M
Baiocco
Cinzia
C
Chiappino
Isabella
I
Castellano
Giancarlo
G
Stacchini
Alessandra
A
Satolli
Maria A
MA
Mele
Michele
M
Sandrucci
Sergio
S
Mussa
Antonio
A
Bisi
Gianni
G
Whiteside
Theresa L
TL