title: Clostridial enteropathy in lactating outbred swiss-derived (ICR) mice.
abstract: Reports of severe enteric disease of unknown etiology affecting lactating mice have appeared in the literature. Clostridial disease similar to that seen in cattle and sheep on high-carbohydrate rations and caused by Clostridium perfringens has been suspected in these mouse outbreaks but has not been isolated from affected mice. The present report describes a severe, necrotizing enterocolitis associated with overgrowth of C. perfringens type A in lactating Swiss-derived (ND4) mice. Mice nursing large litters of pups in the second week of life were the most severely affected. The organism isolated from dead or moribund mice was positive by polymerase chain reaction assay for the gene for the C. perfringens a toxin, but actual toxin production was not determined. The disease in this mouse colony was ameliorated by increasing the fat and calorie content of the diet of lactating dams, which each received 1 g peanut butter every 48 h.
authors.affiliation: Research Animal Resource Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Krugner-Higby
authors.forename: Isabelle
authors.initials: I
authors.lastname: Girard
authors.forename: Janet
authors.initials: J
authors.lastname: Welter
authors.forename: Annette
authors.initials: A
authors.lastname: Gendron
authors.forename: Justin S
authors.initials: JS
authors.lastname: Rhodes
authors.forename: Theodore
authors.initials: T
authors.lastname: Garland
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000235:genetics; D003015:Clostridium Infections / Q000382:microbiology / Q000401:mortality / Q000662:veterinary*; D003016:Clostridium perfringens / Q000302:isolation & purification*; D020345:Enterocolitis, Necrotizing / Q000382:microbiology / Q000401:mortality / Q000662:veterinary*; D005260:Female; D007774:Lactation / Q000502:physiology*; D051379:Mice / Q000382:microbiology*; D008813:Mice, Inbred ICR; D012376:Rodent Diseases / Q000178:diet therapy / Q000382:microbiology* / Q000401:mortality; D010738:Type C Phospholipases / Q000235:genetics
chemical_list: D001427:Bacterial Toxins; D002135:Calcium-Binding Proteins; D010738:Type C Phospholipases; C040649:alpha toxin, Clostridium perfringens