Ethylene could influence ferric reductase, iron transporter, and H+-ATPase gene expression by affecting FER (or FER-like) gene activity.
In previous works, it has been shown, by using ethylene inhibitors and precursors, that ethylene could participate in the regulation of the enhanced ferric reductase activity of Fe-deficient Strategy I plants. However, it was not known whether ethylene regulates the ferric reductase gene expression or other aspects related to this activity. This paper is a study of the effects of ethylene inhibitors and precursors on the expression of the genes encoding the ferric reductases and iron transporters of Arabidopsis thaliana (FRO2 and IRT1) and Lycopersicon esculentum (=Solanum lycopersicum) (FRO1 and IRT1) plants. The effects of ethylene inhibitors and precursors on the activity of the iron reductase and the iron transporter have been examined in parallel. Also studied were the effects of ethylene inhibitors and precursors on the expression of the H(+)-ATPase genes of cucumber (CsHA1 and CsHA2) and the transcription factor genes of tomato (LeFER) and Arabidopsis (AtFRU or AtFIT1, an LeFER homologue) that regulate ferric reductase, iron transporter, and H(+)-ATPse activity. The results obtained suggest that ethylene participates in the regulation of ferric reductase, the iron transporter, and H(+)-ATPase gene expression by affecting the FER (or FER-like) levels.
Lucena
Carlos
C
Department of Agronomy, Edificio Celestino Mutis (C-4), Campus de Rabanales, University of Córdoba, E-14014-Córdoba, Spain.
Waters
Brian M
BM
Romera
F Javier
FJ
García
María José
MJ
Morales
María
M
Alcántara
Esteban
E
Pérez-Vicente
Rafael
R
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism; D018553:Cucumis sativus / Q000235:genetics / Q000378:metabolism; D005030:Ethylenes / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D038181:FMN Reductase / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D007501:Iron / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D027682:Cation Transport Proteins; C494590:ETR1 protein, Arabidopsis; D005030:Ethylenes; C099651:IRT1 protein, Arabidopsis; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011956:Receptors, Cell Surface; D014157:Transcription Factors; C036216:ethylene; D007501:Iron; D038181:FMN Reductase; C023903:ferric citrate iron reductase; D006180:Proton-Translocating ATPases