[Molecular identification of naturally acquired Plasmodium knowlesi infection in a human case].
OBJECTIVE
To confirm the diagnosis of a human case with atypical vivax-malaria from Yunnan Province by molecular technique.


METHODS
DNA was extracted from blood films of unidentified sample, and of four known Plasmodium species (P. vivax, P. falciparum, P. knowlesi, and P. cynomolgi). A DNA-based diagnosis with the polymerase chain reaction (PCR) method targeting the small subunit ribosomal RNA (SSU rRNA) genes of genus- and species-specific (two human malaria species and P. knowlesi) was introduced.


RESULTS
The PCR amplification with primer pair specific for P. knowlesi produced a single fragment of 150 bp. Sequence analysis showed that the amplified fragment was identical to the sequence of P. knowlesi.


CONCLUSIONS
The patient was naturally infected with P. knowlesi.
Zheng
Hui
H
Department of Etiological Biology, the Second Military Medical University, Shanghai, China.
Zhu
Huai-min
HM
Ning
Bei-fang
BF
Li
Xiang-yu
XY
D000328:Adult; D000818:Animals; D002681:China; D016054:DNA, Protozoan / Q000097:blood / Q000302:isolation & purification; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood* / Q000469:parasitology*; D008297:Male; D016789:Plasmodium cynomolgi / Q000235:genetics / Q000302:isolation & purification*; D010963:Plasmodium falciparum / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012342:RNA, Small Nuclear / Q000235:genetics
D016054:DNA, Protozoan; D012342:RNA, Small Nuclear