Endocrine and ovarian responses to prolonged adrenal stimulation at the time of induced corpus luteum regression.
The endocrine and ovarian responses to prolonged adrenal stimulation at the time of corpus luteum (CL) regression were studied in non-lactating non-pregnant Friesian cows. Cows were synchronized with two cloprostenol (PG) injections 11 days apart (second PG referred as time 0). Experiment 1 was carried out on five animals in two phases with a resting period in between. Between -48 and 84 h, animals received 12 injections of either saline (CTR) or adrenocorticotrophic hormone (ACTH) agonist (Synacthen; SYN) every 12 h. Cortisol (C), progesterone (P4), oestradiol (E2) and LH were analysed in the blood samples collected every 8-12 h between days -3 and 4. Pulsatile LH release was studied 4 h before and 4 h after naloxone administration beginning at 96 h. Experiment 2 was carried out on four cows in a cross-over experimental design (two phases, with a resting period in between). Treatments were performed by administering either saline (CTR) or Synacthen (SYN) every 12 h between -36 and 24 h. The concentrations of C, P4 and E2 were measured in blood plasma every 4-12 h from days -3 to 3, then every day from days 5 to 9. In both experiments, ovaries were examined by ultrasonography every 1-3 days. ACTH administration induced a significant increase (p < 0.001) of plasma C lasting for 7 days (experiment 1), and for 3-4 days (experiment 2). Plasma C returned to baseline levels within 6 days (expt 1) or 36 h (expt 2) after treatment interruption. During the SYN phase, LH pre-ovulatory surge was not detectable. During the CTR phase, naloxone administration induced a significant increase (p < 0.05) of average LH concentrations that was not evident during the SYN phase. The dominant follicle development was retarded and mean plasma E2 concentrations were significantly lower during the SYN phase (p < 0.01). Luteolysis was completed within 2 days. However, P4 decline between 0 and 4 h was slower (p < 0.01) during the SYN phase. Our results indicate that, under prolonged adrenal stimulation, follicular development is delayed and LH release is impaired, which are independent of CL function.
Gabai
G
G
Dipartimento di Scienze Sperimentali Veterinarie, Università di Padova, Legnaro (PD), Italy. gianfranco.gabai@unipd.it
Mollo
A
A
Marinelli
L
L
Badan
M
M
Bono
G
G
D000324:Adrenocorticotropic Hormone / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D002417:Cattle / Q000097:blood / Q000502:physiology*; D018592:Cross-Over Studies; D004967:Estrogens / Q000097:blood; D005260:Female; D006728:Hormones / Q000097:blood / Q000494:pharmacology; D006854:Hydrocortisone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood*; D003341:Luteolysis / Q000097:blood / Q000502:physiology*; D009270:Naloxone / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology; D010053:Ovary / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology; D010060:Ovulation / Q000097:blood / Q000187:drug effects / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D014463:Ultrasonography
D004967:Estrogens; D006728:Hormones; D009270:Naloxone; D011374:Progesterone; D000324:Adrenocorticotropic Hormone; D007986:Luteinizing Hormone; D006854:Hydrocortisone