Identification and quantification of methanogenic Archaea in adult chicken ceca.
By using molecular methods for the identification and quantification of methanogenic archaea in adult chicken ceca, 16S rRNA genes of 11 different phylotypes, 10 of which were 99% similar to Methanobrevibacter woesei, were found. Methanogen populations, as assessed by cultivation, and the 16S rRNA copy number were between 6.38 and 8.23 cells/g (wet weight) and 5.50 and 7.19 log(10)/g (wet weight), respectively.
Saengkerdsub
Suwat
S
University of Arkansas, Department of Food Science, 2650 N. Young Avenue, Fayetteville, AR 72704-5690, USA.
Anderson
Robin C
RC
Wilkinson
Heather H
HH
Kim
Woo-Kyun
WK
Nisbet
David J
DJ
Ricke
Steven C
SC
D000818:Animals; D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002432:Cecum / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D019641:DNA, Archaeal / Q000032:analysis; D005260:Female; D008697:Methane / Q000378:metabolism*; D041581:Methanobrevibacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S; D008697:Methane