[Study of dicyclohexyl phthalate on preparation and characterization of artificial antigen].
OBJECTIVE
To study the preparation and characterization of hapten and artificial antigen of dicyclohexyl phthalate.


METHODS
A dicyclohexyl phthalate hapten (4-amino dicyclohexyl phthalate) was synthesized by introducing amino as a substituent on the aromatic ring and retaining the ester group, and characterized by 1HNMR, IR and UV. The hapten was conjugated to BSA via amino diazotization linkage.


RESULTS
Lambda1 = 214nm, lambda2 = 256nm for the UV of dicyclohexyl 4-nitrophthalate and lambda1 = 226nm, lambda2 = 288nm for the UV of dicyclohexyl 4-aminophthalate. Artificial antigen was prepared and tested by fluorescence, and lambda(ex) = 307nm, lambda(em) = 468nm, and the approximate molar ratio of dicyclohexyl 4-aminophthalate to BSA was 19. The product was used as an immunogen, demonstrating that it is suitable for polyclonal antibody production.


CONCLUSIONS
It is a good method for preparation of artificial antigen of dicyclohexyl phthalate by introducing amino as a substituent on the aromatic ring and retaining the ester group. It was suggested that could supply excellent immune antigen for further preparation of antibody and immunoassay to dicyclohexyl phthalate.
Zhang
Ming-Cui
MC
College of Environmental Science and Engineering, Donghua University, Shanghai 200051, China.
Zhuang
Hui-Sheng
HS
Lang
Qing
Q
D000818:Animals; D000941:Antigens / Q000276:immunology*; D008297:Male; D010795:Phthalic Acids / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits
D000941:Antigens; D010795:Phthalic Acids; C036042:dicyclohexyl phthalate