title: Murine and HIV-based retroviral vectors for in vitro and in vivo gene transfer.
abstract: The success of experimental gene therapy is dependent on the ability to safely and efficiently introduce transgenes into the target cell or tissue. Retroviral-based vectors, notably those derived from Moloney murine leukemia virus (MLV) and lentiviral vectors derived from HIV, have proven to be valuable gene transfer vehicles as a result of their ease of production and their ability to mediate long-term transgene expression. One of the most widely used methods for viral vector production is based on the transient transfection of viral vector plasmid DNA into a producer cell line. Here, we describe protocols to produce and standardize high quality MLV-based retroviral and HIV-based lentiviral vectors for ex vivo and in vivo gene delivery.
authors.affiliation: Department of Neurosciences, University of California, San Diego, La Jolla, USA.
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Alfa
authors.forename: Armin
authors.initials: A
authors.lastname: Blesch
mesh_terms: D000818:Animals; D002318:Cardiovascular Diseases / Q000628:therapy; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006678:HIV; D009052:Leukemia Virus, Murine; D051379:Mice