title: Comparison of ELISA and immunoassays for measurement of IgG and IgM antibody to West Nile virus in human sera against virus neutralisation.
abstract: Two commercial assays for the detection of IgG antibody to West Nile virus (WNV), an indirect enzyme-linked immunosorbent assay (I-ELISA) and indirect fluorescent antibody test (IFAT), were evaluated against the virus neutralisation test. Excellent agreement with the virus neutralisation was obtained with both tests, i.e., 99.5% by I-ELISA and 100% by IFAT. The well-known serological cross-reactivity within the family of the Flaviviridae was analysed using sera with known antibodies against dengue virus, tick borne encephalitis virus and yellow fever virus. IgM and/or IgG positive sera were examined for reactivity by WNV-ELISA and WNV-IFAT. While cross-reactivity between 0 and 18.2% was recorded with IgM positive sera, there was extensive cross-reactivity of 15.7-100% with IgG positive sera.
authors.affiliation: Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany. niedrigm@rki.de
authors.forename: M
authors.initials: M
authors.lastname: Niedrig
authors.forename: K
authors.initials: K
authors.lastname: Sonnenberg
authors.forename: K
authors.initials: K
authors.lastname: Steinhagen
authors.forename: J T
authors.initials: JT
authors.lastname: Paweska
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D009500:Neutralization Tests; D014902:West Nile virus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M