The stimulating effects of ethylene and auxin on petiole elongation and on hyponastic curvature are independent processes in submerged Rumex palustris.
The flooding-tolerant plant species Rumex palustris (Sm.) responds to complete submergence with stimulation of petiole elongation mediated by the gaseous hormone ethylene. We examined the involvement of auxin in petiole elongation. The manipulation of petiolar auxin levels by removing the leaf blade, or by addition of synthetic auxins or auxin transport inhibitors, led to the finding that auxin plays an important role in submergence-induced petiole elongation in R. palustris. A detailed kinetic analysis revealed a transient effect of removing the auxin source (leaf blade), explaining why earlier studies in which less frequent measurements were taken failed to identify any role for auxin in petiole elongation. We previously showed that the onset of stimulated petiole elongation depends on a more upright petiole angle being reached by means of hyponastic (upward) curvature, a differential growth process that is also regulated by ethylene and auxin. This raised the possibility that both ethylene and auxin stimulate elongation only indirectly by influencing hyponastic growth. We show here that the action of ethylene and auxin in promoting petiole elongation in submerged R. palustris is independent of the promoting effect that these hormones also exert on the hyponastic curvature of the same petiole.
Cox
Marjolein C H
MC
Plant Ecophysiology, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, the Netherlands.
Peeters
Anton J M
AJ
Voesenek
Laurentius A C J
LA
D000598:Amino Acids, Cyclic / Q000502:physiology; D005030:Ethylenes / Q000378:metabolism*; D005875:Gibberellins / Q000502:physiology; D007101:Immersion; D007210:Indoleacetic Acids / Q000378:metabolism*; D018515:Plant Leaves / Q000254:growth & development*; D031804:Rumex / Q000254:growth & development*; D014867:Water / Q000502:physiology*
D000598:Amino Acids, Cyclic; D005030:Ethylenes; D005875:Gibberellins; D007210:Indoleacetic Acids; D014867:Water; C023863:1-aminocyclopropane-1-carboxylic acid; C036216:ethylene; C007842:gibberellic acid