New crystal forms of Diocleinae lectins in the presence of different dimannosides.
Studying the interactions between lectins and sugars is important in order to explain the differences observed in the biological activities presented by the highly similar proteins of the Diocleinae subtribe. Here, the crystallization and preliminary X-ray data of Canavalia gladiata lectin (CGL) and C. maritima lectin (CML) complexed with Man(alpha1-2)Man(alpha1)OMe, Man(alpha1-3)Man(alpha1)OMe and Man(alpha1-4)Man(alpha1)OMe in two crystal forms [the complexes with Man(alpha1-3)Man(alpha1)OMe and Man(alpha1-4)Man(alpha1)OMe crystallized in space group P3(2) and those with Man(alpha1-2)Man(alpha1)OMe crystallized in space group I222], which differed from those of the native proteins (P2(1)2(1)2 for CML and C222 for CGL), are reported. The crystal complexes of ConA-like lectins with Man(alpha1-4)Man(alpha1)OMe are reported here for the first time.
Moreno
Frederico Bruno Mendes Batista
FB
Departamento de Física-IBILCE-Universidade Estadual Paulista, São José do Rio Preto 15054-000, Brazil.
Bezerra
Gustavo Arruda
GA
de Oliveira
Taianá Maia
TM
de Souza
Emmanuel Prata
EP
da Rocha
Bruno Anderson Matias
BA
Benevides
Raquel Guimarães
RG
Delatorre
Plínio
P
Cavada
Benildo Sousa
BS
de Azevedo
Walter Filgueira
WF
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry; D018360:Crystallography, X-Ray; D007887:Fabaceae / Q000737:chemistry*; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry*
D002241:Carbohydrates; D037121:Plant Lectins