Absence of CD4+ T cells impairs host defence of mice infected with Fonsecaea pedrosoi.
Chromoblastomycosis is a human chronic, often debilitating, suppurative, granulomatus mycosis of the skin and subcutaneous tissues beginning after inoculation trauma. It occurs worldwide, but is more frequently observed in tropical countries such as Brazil. Some studies have focused on fungus-host interaction, showing a predominantly cell-mediated immune response, with the activation of macrophages involved in fungus phagocytosis. Immunization with live conidia produced a high influx of CD4 T cells into the draining lymph node. The sensitized T cells proliferate in vitro when restimulated with specific antigen and preferentially produce IFN- gamma. To better characterize the role played by T cells on the chromoblastomycosis infection we used mice deficient for CD4 and CD8. Data determined by CFU counts associated with decreased DTH and IFN-gamma production of infected mice clearly demonstrated that, during experimental F. pedrosoi infection, absence of CD4(+) cells induces a more severe disease.
Teixeira de Sousa
M da Gloria
Mda G
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.
Ghosn
E E B
EE
Almeida
S R
SR
D000818:Animals; D001203:Ascomycota; D001681:Biological Assay; D015704:CD4 Antigens / Q000235:genetics; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016827:CD8 Antigens / Q000235:genetics; D002862:Chromoblastomycosis / Q000235:genetics / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma; D008212:Lymphocyte Depletion; D051379:Mice; D008817:Mice, Mutant Strains
D015704:CD4 Antigens; D016827:CD8 Antigens; D007371:Interferon-gamma