Determination of doxycycline in pharmaceuticals and human urine by micellar electrokinetic capillary chromatography.
Micellar electrokinetic capillary chromatography (MEKC) was performed at 25 degrees C and 30 kV (under a pressure of 15 mbar), using 30 mM borate buffer containing 60 mM sodium dodecysulfate (SDS) and 5% (v/v) methanol as background electrolyte (pH 9.0) to determine doxycycline. UV detection was at 350 nm. The method was shown to be specific, accurate (recovery was 100.3 +/- 1.0%), linear over the tested range (correlation coefficient 0.9995) and precise (RSD <1.9%). The method was used to determine doxycycline in tablets, capsules and human urine after oral application.
Injac
Rade
R
Institute of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000, Ljubljana, Slovenia. injac@email.com
Kac
Javor
J
Kreft
Samo
S
Strukelj
Borut
B
D000900:Anti-Bacterial Agents; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods* / Q000592:standards; D004318:Doxycycline / Q000032:analysis* / Q000652:urine*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D004364:Pharmaceutical Preparations; D004318:Doxycycline