[Research in the relation between telomerase reverse transcriptase expression in spermatogenic cells and serum levels of estradiol of fluorotic rats].
OBJECTIVE
To study the relation between telomerase reverse transcriptase (TERT) activity expression in spermatogenic cells and serum levels of estradiol of fluorotic rats.


METHODS
We randomly divided thirty SD male rats into control group, low-dose group and high-dose group, then inject sodium fluoride (0, 10, 20 mg/kg bw) into celiac of rats. We respectively observed changes of estrogen and TERT using methods of radioimmunoassay, in situ hybridization. In addition, we observed the quality of spermatozoa.


RESULTS
The level of estrogen, the expression of telomerase and the number and the livability of the spermatozoon in low-dose and high-dose fluorotic rats were lower than those of control rats (P < 0.05). Therefore,the above indexes decreased with the increase of dosage. In addition, sperm aberration of each fluorotic group was higher than control group (P < 0.05). And it increased with the increase of dosage. The content of E2 in serum of different fluoride treatment groups was positively correlated with the expression of telomerase in seminiferous tubule significantly, respectively (low-dose fluoride treatment groups, r = 0.941, P < 0.01, high-dose fluoride treatment groups, r = 0.929, P < 0.01).


CONCLUSIONS
NaF possibly damaged the male reproductive system by the approach of E2/ER-TERT-spermatozoon, relation between TERT expression in spermatogenic cells and serum levels of estradiol is positive correlation.
Li
Yan
Y
Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou 450052, China.
Zhu
Jing-Yuan
JY
Zhuang
Dong-Gang
DG
Cheng
Xue-Min
XM
Jiang
Qin
Q
Ba
Yue
Y
Zhang
Hui-zhen
HZ
Cui
Liu-xin
LX
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000097:blood*; D005461:Fluorine / Q000633:toxicity*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013076:Sperm Count; D013087:Spermatids / Q000187:drug effects* / Q000201:enzymology; D019098:Telomerase / Q000096:biosynthesis*
D005461:Fluorine; D004958:Estradiol; D019098:Telomerase