title: Lipid peroxidation in ischemia-reperfusion oxidative injury of the graft preserved in Celsior and University of Wisconsin solutions on a pig pancreas transplantation model.
abstract: BACKGROUND
  Graft pancreatitis (GP) is one the main technical problems associated with pancreas transplant (PT). It occurs in 20% of patients representing a risk factor for thrombosis and cause of graft loss. GP is related to oxidative effects from oxygen-derived free radicals (OFR) in ischemia-reperfusion injury. We evaluated lipid peroxidation by the OFR in the PT of pig organs preserved with either Celsior or Wisconsin solutions.
  
  
  METHODS
  In Landrace pigs we performed 24 pancreas allografts, which were preserved 18 or 24 hours: 12 with Celsior solution (CS) and 12 with Wisconsin solution (UW). No immunosuppression was administered. The oxidative effects were determined by quantification of malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) and of the carbonyl groups of proteins in our pancreatic tissue samples and measured at different times: (A) baseline in the donor, (B) after perfusion of the graft, (C) after the ischemia period, and (D) 30 minutes after ischemia-reperfusion of the graft.
  
  
  RESULTS
  The MDA and 4-HDA values were similar in conditions A, B, and C, but showed an extraordinary increase after ischemia-reperfusion in D, among both the 18- or 24-hour preserved grafts and in the same proportion with CS and UW. The carbonyl groups of the proteins rose in conditions B and C (cold ischemia), but less so in state D (reperfusion).
  
  
  CONCLUSIONS
  The oxidative injury of a pancreatic graft preserved for 18 or 24 hours occurs during reperfusion, with an extraordinary intensity, but similarly with CS and UW, an observation that may help to explain graft pancreatitis.
authors.affiliation: Liver Transplant Unit, Hospital Clínico Universitario "Lozano Blesa," Zaragoza, Spain. agarciagil@telefonica.net
authors.forename: F A
authors.initials: FA
authors.lastname: Garcia-Gil
authors.forename: E
authors.initials: E
authors.lastname: Gonzalvo
authors.forename: J J
authors.initials: JJ
authors.lastname: Garcia-Garcia
authors.forename: C D
authors.initials: CD
authors.lastname: Albendea
authors.forename: A
authors.initials: A
authors.lastname: Güemes
authors.forename: E
authors.initials: E
authors.lastname: Tome-Zelaya
authors.forename: L
authors.initials: L
authors.lastname: Fuentes
authors.forename: E
authors.initials: E
authors.lastname: Santa-Clotilde
authors.forename: J
authors.initials: J
authors.lastname: Aso
authors.forename: C
authors.initials: C
authors.lastname: Bejarano
authors.forename: N
authors.initials: N
authors.lastname: Garrido
authors.forename: C
authors.initials: C
authors.lastname: Garcia
authors.forename: E
authors.initials: E
authors.lastname: Gómez
authors.forename: M
authors.initials: M
authors.lastname: Sánchez
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D005978:Glutathione; D006639:Histidine; D007328:Insulin; D008353:Mannitol; D023421:Models, Animal; D019188:Organ Preservation Solutions; D018384:Oxidative Stress; D010179:Pancreas; D016035:Pancreas Transplantation / Q000009:adverse effects / Q000379:methods* / Q000502:physiology; D011183:Postoperative Complications / Q000145:classification / Q000517:prevention & control; D011887:Raffinose; D015427:Reperfusion Injury / Q000145:classification / Q000517:prevention & control*; D013552:Swine
chemical_list: C088453:Celsior; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D006639:Histidine; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose