Changes in gene expression pattern following granulocyte colony-stimulating factor administration to normal stem cell sibling donors.
Granulocyte colony-stimulating factor (G-CSF) is widely used for the mobilization of hematopoietic stem cells from normal donors. The mechanism of induced mobilization has recently been elucidated. Treatment with G-CSF is considered safe; however, long-term effects are largely unknown. The aim of this study was, therefore, to determine whether it leads to significant changes in gene expression modifications. Affymetrix Gene Chip array technology was used. Samples were collected before and at various time points (up to 9 months) after mobilization. The expression levels of 122 genes were transiently modified before and after mobilization (p < 0.05). Fifty-three genes belonging to cell growth, proliferation and communication gene ontology categories were upregulated, while 69 genes were downregulated.


CONCLUSIONS
The administration of G-CSF is associated with transient DNA and gene expression modifications in the lymphocytes of normal mobilized donors. A long-term follow-up of stem cell donors is recommended.
Amariglio
Ninette
N
Division of Hematology, Chaim Sheba Medical Center, Tel Hashomer, Israel.
Jacob-Hirsch
Jasmine
J
Shimoni
Avichai
A
Leiba
Merav
M
Rechavi
Gideon
G
Nagler
Arnon
A
D015536:Down-Regulation; D005786:Gene Expression Regulation / Q000187:drug effects*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000009:adverse effects / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D035781:Siblings; D033581:Stem Cell Transplantation; D014019:Tissue Donors; D015854:Up-Regulation
D016179:Granulocyte Colony-Stimulating Factor