CD2 promoter regulated nucleophosmin-anaplastic lymphoma kinase in transgenic mice causes B lymphoid malignancy.
BACKGROUND
Nucleophosmin-anaplastic lymphoma kinase expression is associated with a lymphoid malignancy, anaplastic large cell lymphoma, and is characterized by a t(2;5) chromosomal translocation.


METHODS
We describe a novel transgenic mouse line in which NPM-ALK expression is targeted to the T-cell lineage using the CD2 promoter.


RESULTS
Surprisingly, the mice develop B cell lymphomas in the majority of cases.


CONCLUSIONS
These data stress the importance of choice of promoter to drive transgene expression in obtaining the desired phenotype.
Turner
Suzanne D
SD
Laboratory of Lymphocyte Signalling and Development, The Babraham Institute, Babraham Research Campus, Babraham, Cambridge CB2 4AT, UK. Sdt36@cam.ac.uk
Merz
Hartmut
H
Yeung
Debra
D
Alexander
Denis R
DR
D000818:Animals; D015153:Blotting, Western; D018801:CD2 Antigens / Q000235:genetics*; D045744:Cell Line, Tumor; D019070:Cell Lineage; D005260:Female; D005838:Genotype; D006801:Humans; D016393:Lymphoma, B-Cell / Q000201:enzymology / Q000209:etiology* / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000201:enzymology / Q000209:etiology* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000201:enzymology / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D015514:Oncogene Proteins, Fusion / Q000502:physiology; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases / Q000502:physiology*
D018801:CD2 Antigens; D015514:Oncogene Proteins, Fusion; C107023:p80(NPM-ALK) protein; D011505:Protein-Tyrosine Kinases