title: Retinal ganglion cell loss induced by acute optic neuritis in a relapsing model of multiple sclerosis.
abstract: Multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE) are marked by inflammatory demyelinating lesions throughout the central nervous system, including optic nerve. Neuronal loss also occurs in MS and EAE lesions, but it is not known whether neuronal loss occurs secondary to inflammation, or as a primary process. In the current study, the relationship of inflammation to retinal ganglion cell (RGC) loss during acute optic neuritis is examined. RGCs were labelled with Flourogold, and EAE was induced in SJL/J mice by immunization with proteolipid protein peptide 139-151 (PLP). At various time points, RGCs were counted and optic nerves were examined for inflammatory cell infiltrates. No optic neuritis was detected prior to day 9 following immunization. Incidence of optic neuritis was 30% at day 9 and increased to over 70% by day 11, remaining high through day 18. In contrast, no RGC loss was detected in eyes with optic neuritis until day 14. A 43.1% reduction in RGC numbers at day 14 increased to 50.8% by day 18. No RGC loss occurred in eyes without optic neuritis. The fact that inflammation precedes RGC loss suggests that neuronal loss during optic neuritis occurs secondary to the inflammatory process.
authors.affiliation: Department of Ophthalmology, University of Pennsylvania, Scheie Eye Institute, 51 N. 39th Street, Philadelphia, PA 19104, USA. kenneth.shindler@uphs.upenn.edu
authors.forename: Kenneth S
authors.initials: KS
authors.lastname: Shindler
authors.forename: Yangtai
authors.initials: Y
authors.lastname: Guan
authors.forename: Elvira
authors.initials: E
authors.lastname: Ventura
authors.forename: Jean
authors.initials: J
authors.lastname: Bennett
authors.forename: Abdolmohamad
authors.initials: A
authors.lastname: Rostami
mesh_terms: D000818:Animals; D002452:Cell Count / Q000379:methods; D004195:Disease Models, Animal; D005260:Female; D005620:Freund's Adjuvant; D051379:Mice; D009103:Multiple Sclerosis / Q000139:chemically induced / Q000150:complications*; D018991:Myelin Proteolipid Protein; D009902:Optic Neuritis / Q000209:etiology* / Q000473:pathology*; D010446:Peptide Fragments; D012165:Retinal Ganglion Cells / Q000473:pathology*; D013997:Time Factors
chemical_list: D018991:Myelin Proteolipid Protein; D010446:Peptide Fragments; C435023:myelin proteolipid protein (139-151); D005620:Freund's Adjuvant