HLA-DRB1*04 alleles in Japanese rheumatoid arthritis patients with AA amyloidosis.
OBJECTIVE
To compare the HLA-DRB1 shared epitope (SE) alleles in Japanese patients with rheumatoid arthritis (RA) and amyloid A (AA) amyloidosis versus those without AA amyloidosis.


METHODS
The HLA-DRB1 alleles were genotyped for 91 RA patients without AA amyloidosis, 33 RA patients with AA amyloidosis, and 63 control subjects. HLA-DRB1 typing was performed by polymerase chain reaction, sequence-specific oligonucleotide probe hybridization method.


RESULTS
Although a significant difference was not observed, the frequency of SE genotype was higher in RA patients with AA amyloidosis than in those without AA amyloidosis. All SE-positive RA patients with AA amyloidosis had *04 alleles (*0401, *0405, *0410), and a significant association of the presence of a double dose of *04 SE alleles with AA amyloidosis (OR 4.0, 95% CI 1.91-13.99) was observed.


CONCLUSIONS
Our data suggest that presence of double *04 SE is associated with a higher risk of developing AA amyloidosis in Japanese patients with RA.
Migita
Kiyoshi
K
Clinical Research Center, NHO Nagasaki Medical Center, Kubara 2-1001-1, Omura 856-8652, Japan. migita@nmc.hosp.go.jp
Nakamura
Tadashi
T
Maeda
Yumi
Y
Miyashita
Taichiro
T
Origuchi
Tomoki
T
Yatsuhashi
Hiroshi
H
Nakamura
Minoru
M
Ishibashi
Hiromi
H
Eguchi
Katsumi
K
D000368:Aged; D000686:Amyloidosis / Q000208:ethnology / Q000209:etiology / Q000235:genetics*; D001172:Arthritis, Rheumatoid / Q000150:complications / Q000208:ethnology / Q000235:genetics*; D044466:Asian People; D016022:Case-Control Studies; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D059811:HLA-DRB1 Chains; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D000685:Serum Amyloid A Protein / Q000235:genetics
D000939:Epitopes; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D000685:Serum Amyloid A Protein