title: Respiratory nitrate ammonification by Shewanella oneidensis MR-1.
abstract: Anaerobic cultures of Shewanella oneidensis MR-1 grown with nitrate as the sole electron acceptor exhibited sequential reduction of nitrate to nitrite and then to ammonium. Little dinitrogen and nitrous oxide were detected, and no growth occurred on nitrous oxide. A mutant with the napA gene encoding periplasmic nitrate reductase deleted could not respire or assimilate nitrate and did not express nitrate reductase activity, confirming that the NapA enzyme is the sole nitrate reductase. Hence, S. oneidensis MR-1 conducts respiratory nitrate ammonification, also termed dissimilatory nitrate reduction to ammonium, but not respiratory denitrification.
authors.affiliation: Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824-1325, USA.
authors.forename: Claribel
authors.initials: C
authors.lastname: Cruz-García
authors.forename: Alison E
authors.initials: AE
authors.lastname: Murray
authors.forename: Joel A
authors.initials: JA
authors.lastname: Klappenbach
authors.forename: Valley
authors.initials: V
authors.lastname: Stewart
authors.forename: James M
authors.initials: JM
authors.lastname: Tiedje
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D000693:Anaerobiosis; D016680:Genome, Bacterial; D009154:Mutation; D050901:Nitrate Reductase / Q000235:genetics / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D010084:Oxidation-Reduction; D020592:Shewanella / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D009566:Nitrates; D009573:Nitrites; D000641:Ammonia; D050901:Nitrate Reductase