A novel and in situ technique for the quantitative detection of MTBE and benzene degrading bacteria in contaminated matrices.
A novel and in situ technique is presented here as a better alternative to culture-dependent and PCR-based techniques for the quantitative detection of predominant bacterial species involved in the bioremediation of contaminants. It allowed rapid, specific and in situ identification of Biosep-immobilized eubacteria from MTBE- and benzene-contaminated matrices.
Biggerstaff
J P
JP
University of Tennessee, Knoxville, TN, United States. jbiggers@utk.edu
Le Puil
M
M
Weidow
B L
BL
Leblanc-Gridley
J
J
Jennings
E
E
Busch-Harris
J
J
Sublette
K L
KL
White
D C
DC
Alberte
R S
RS
D001554:Benzene / Q000378:metabolism*; D001673:Biodegradation, Environmental; D020564:Deltaproteobacteria / Q000302:isolation & purification / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008738:Methyl Ethers / Q000032:analysis* / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D008738:Methyl Ethers; D012989:Soil Pollutants; C043243:methyl tert-butyl ether; D001554:Benzene