Luminol-hydrogen peroxide chemiluminescence produced by sweet potato peroxidase.
Anionic sweet potato peroxidase (SPP; Ipomoea batatas) was shown to efficiently catalyse luminol oxidation by hydrogen peroxide, forming a long-term chemiluminescence (CL) signal. Like other anionic plant peroxidases, SPP is able to catalyse this enzymatic reaction efficiently in the absence of any enhancer. Maximum intensity produced in SPP-catalysed oxidation of luminol was detected at pH 7.8-7.9 to be lower than that characteristic of other peroxidases (8.4-8.6). Varying the concentrations of luminol, hydrogen peroxide and Tris buffer in the reaction medium, we determined favourable conditions for SPP catalysis (100 mmol/L Tris-HCl buffer, pH 7.8, containing 5 mmol/L hydrogen peroxide and 8 mmol/L luminol). The SPP detection limit in luminol oxidation was 1.0 x 10(-14) mol/L. High sensitivity in combination with the long-term CL signal and high stability is indicative of good promise for the application of SPP in CL enzyme immunoassay.
Alpeeva
Inna S
IS
Chemical Enzymology Department, Faculty of Chemistry, The M.V. Lomonosov Moscow State University, Moscow, 119992, Russia.
Yu Sakharov
Ivan
I
D002138:Calibration; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D027723:Ipomoea batatas / Q000201:enzymology*; D007700:Kinetics; D049449:Luminescence; D008165:Luminol / Q000737:chemistry*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry*
D008165:Luminol; D006861:Hydrogen Peroxide; D009195:Peroxidase