Purification and assay of the human INO80 and SRCAP chromatin remodeling complexes.
Chromatin modifying and remodeling enzymes play critical roles in many aspects of chromosome biology including transcription, replication, recombination, and repair. Our laboratory recently identified and characterized two multisubunit human chromatin remodeling enzymes designated the INO80 and SRCAP complexes. Mechanistic studies revealed that the human INO80 complex catalyzes nucleosome sliding and the SRCAP complex catalyzes ATP-dependent exchange of histone H2A/H2B dimers containing the histone variant H2A.Z into nucleosomes. Here we describe methods for purification and assay of the INO80 and SRCAP chromatin remodeling complexes.
Cai
Yong
Y
Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
Jin
Jingji
J
Gottschalk
Aaron J
AJ
Yao
Tingting
T
Conaway
Joan W
JW
Conaway
Ronald C
RC
D000251:Adenosine Triphosphatases / Q000302:isolation & purification*; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D002846:Chromatography, Affinity / Q000379:methods*; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D006801:Humans; D046912:Multiprotein Complexes / Q000302:isolation & purification*; D009707:Nucleosomes / Q000378:metabolism; D014157:Transcription Factors / Q000302:isolation & purification*; D000092468:Bromodomain Containing Proteins
D000251:Adenosine Triphosphatases; D002868:Chromosomal Proteins, Non-Histone; D046912:Multiprotein Complexes; D009707:Nucleosomes; D014157:Transcription Factors; C492319:BAZ1A protein, human; D000092468:Bromodomain Containing Proteins; C119652:SRCAP protein, human