title: Analysis of microsatellite instability in stool DNA of patients with colorectal cancer using denaturing high performance liquid chromatography.
abstract: OBJECTIVE
  To evaluate the usefulness of denaturing high performance liquid chromatography (DHPLC) for analyzing microsatellite instability (MSI) status in stool DNA of patients with colorectal cancer.
  
  
  METHODS
  A total of 80 cancer tissues from patients with primary sporadic colorectal tumor (proximal cancer: 27, distal cancer: 53) and matched stool (which were employed for comparison with the tissues) were analyzed for MSI status in BAT 26. DNA samples extracted from stool were evaluated by nested polymerase chain reaction (PCR) and DHPLC for MSI analysis.
  
  
  RESULTS
  Six cases (7.5%) of MSI were identified in BAT 26 from 80 cancer tissues. All the stool DNA samples from patients whose cancer tissue showed MSI also displayed MSI in BAT 26.
  
  
  CONCLUSIONS
  As MSI is one of the established fecal DNA markers to screen colorectal cancer, we propose to use DHPLC for the MSI analysis in fecal DNA.
authors.affiliation: Research Institute and Hospital, National Cancer Center, Goyang, and Cancer Research Institute and Cancer Research Center, Seoul National University, Seoul, Korea.
authors.forename: Seok-Byung
authors.initials: SB
authors.lastname: Lim
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Jeong
authors.forename: Il-Jin
authors.initials: IJ
authors.lastname: Kim
authors.forename: Dae Yong
authors.initials: DY
authors.lastname: Kim
authors.forename: Kyung Hae
authors.initials: KH
authors.lastname: Jung
authors.forename: Hee Jin
authors.initials: HJ
authors.lastname: Chang
authors.forename: Hyo Seong
authors.initials: HS
authors.lastname: Choi
authors.forename: Dae Kyung
authors.initials: DK
authors.lastname: Sohn
authors.forename: Hio Chung
authors.initials: HC
authors.lastname: Kang
authors.forename: Yong
authors.initials: Y
authors.lastname: Shin
authors.forename: Sang-Geun
authors.initials: SG
authors.lastname: Jang
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Jae-Gahb
authors.initials: JG
authors.lastname: Park
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015179:Colorectal Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D005243:Feces / Q000737:chemistry; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D053842:Microsatellite Instability
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm