title: Partial-filling affinity capillary electrophoresis techniques to probe the binding of glycopeptide antibiotics to D-Ala-D-Ala terminus peptides.
abstract: This work is an overview of our use of affinity capillary electrophoresis (ACE) to estimate binding constants between D-Ala-D-Ala terminus peptides and the glycopeptides vancomycin (Van) from Streptomyces orientalis, teicoplanin (Teic) from Actinoplanes teicomyceticus, and ristocetin A (Rist) from Nocardia lurida. In these studies, modifications in the ACE technique, including partial-filling ACE (PFACE), flow-through PFACE (FTPFACE), on-column ligand derivatization ACE (OCLDACE), on-column receptor derivatization ACE (OCRDACE), multiple-step ligand injection PFACE (MSLIPFACE), and multiple-injection ACE (MIACE), are described and used to determine binding constants of peptides to antibiotics. The findings described herein demonstrate the advantages of ACE in estimating binding parameters between antibiotics and small peptides over other analytical techniques.
authors.affiliation: Department of Chemistry and Biochemistry, California State University, Los Angeles, CA 90032-8202, USA.
authors.forename: Jose
authors.initials: J
authors.lastname: Zavaleta
authors.forename: Dinora B
authors.initials: DB
authors.lastname: Chinchilla
authors.forename: Catherine F
authors.initials: CF
authors.lastname: Kaddis
authors.forename: Karla
authors.initials: K
authors.lastname: Martinez
authors.forename: Abby
authors.initials: A
authors.lastname: Brown
authors.forename: Alvaro
authors.initials: A
authors.lastname: Gomez
authors.forename: Amaris
authors.initials: A
authors.lastname: Pao
authors.forename: Alejandra
authors.initials: A
authors.lastname: Ramirez
authors.forename: Sanjay
authors.initials: S
authors.lastname: Nilapwar
authors.forename: John E
authors.initials: JE
authors.lastname: Ladbury
authors.forename: Frank A
authors.initials: FA
authors.lastname: Gomez
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006020:Glycopeptides / Q000032:analysis* / Q000737:chemistry; D015336:Molecular Probe Techniques; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D013048:Specimen Handling / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D006020:Glycopeptides; D010455:Peptides