Rapid molecular strategy for filovirus detection and characterization.
Filoviruses have the capacity to cause lethal outbreaks of hemorrhagic fever in primates. Here we present a simple consensus reverse transcription-PCR method for filovirus recognition and characterization and demonstrate its utility with all known filovirus strains. Phylogenetic assignment is achieved by automated web-based sequence analysis of amplification products.
Zhai
Junhui
J
Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, New York, NY 10032, USA.
Palacios
Gustavo
G
Towner
Jonathan S
JS
Jabado
Omar
O
Kapoor
Vishal
V
Venter
Marietjie
M
Grolla
Allen
A
Briese
Thomas
T
Paweska
Janusz
J
Swanepoel
Robert
R
Feldmann
Heinz
H
Nichol
Stuart T
ST
Lipkin
W Ian
WI
D000818:Animals; D029043:Ebolavirus / Q000235:genetics / Q000302:isolation & purification; D016563:Filoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D018702:Filoviridae Infections / Q000175:diagnosis* / Q000821:virology*; D019142:Hemorrhagic Fever, Ebola; D006801:Humans; D008379:Marburg Virus Disease; D029024:Marburgvirus / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors