Hybridization of DNA to bead-immobilized probes confined within a microfluidic channel.
We report the factors influencing the capture of DNA by DNA-modified microbeads confined within a microfluidic channel. Quantitative correlation of target capture efficiency to probe surface concentration, solution flow rate, and target concentration are discussed. The results indicate that the microfluidic system exhibits a limit of detection of approximately 10(-10) M (approximately 10(-16) mol) DNA and a selectivity factor of approximately 8 x 10(3). Typical hybridization times are on the order of minutes.
Kim
Joohoon
J
Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station, A5300, Austin, Texas 78712-0165, USA.
Heo
Jinseok
J
Crooks
Richard M
RM
D019921:Biotinylation; D004247:DNA / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D044085:Microfluidics / Q000379:methods*; D008863:Microspheres; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000737:chemistry; D012984:Software; D013499:Surface Properties
D015345:Oligonucleotide Probes; D004247:DNA