Detection of galectin-3 by novel peptidic photoprobes.
Photoprobes were prepared with specificity for binding, labeling, and visualizing galectin-3 in a mixture of proteins. The probes were derived from a galectin-3 binding 15-mer peptide sequence in which a benzophenone photolabel was incorporated at the N-terminus and in another case as a phenyl alanine replacement in the middle of the sequence. Detection of galectin-3 was possible in Escherichia coli lysates that were spiked with various amounts of galectin-3.
van Scherpenzeel
Monique
M
Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80082, 3508 TB Utrecht, The Netherlands.
van der Pot
Martin
M
Arnusch
Christopher J
CJ
Liskamp
Rob M J
RM
Pieters
Roland J
RJ
D001577:Benzophenones / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000737:chemistry; D037502:Galectin 3 / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D010777:Photochemistry
D001577:Benzophenones; D037502:Galectin 3; D007202:Indicators and Reagents; D010455:Peptides