Mapping antigenic diversity and strain specificity of mumps virus: a bioinformatics approach.
Mumps is an acute infectious disease caused by mumps virus, a member of the family Paramyxoviridae. With the implementation of vaccination programs, mumps infection is under control. However, due to resurgence of mumps epidemics, there is a renewed interest in understanding the antigenic diversity of mumps virus. Hemagglutinin-neuraminidase (HN) is the major surface antigen and is known to elicit neutralizing antibodies. Mutational analysis of HN of wild-type and vaccine strains revealed that the hypervariable positions are distributed over the entire length with no detectable pattern. In the absence of experimentally derived 3D structure data, the structure of HN protein of mumps virus was predicted using homology modeling. Mutations mapped on the predicted structures were found to cluster on one of the surfaces. A predicted conformational epitope encompasses experimentally characterized epitopes suggesting that it is a major site for neutralization. These analyses provide rationale for strain specificity, antigenic diversity and varying efficacy of mumps vaccines.
Kulkarni-Kale
Urmila
U
Bioinformatics Centre, University of Pune, Pune 411007, India. urmila@bioinfo.ernet.in
Ojha
Janaki
J
Manjari
G Sunitha
GS
Deobagkar
Deepti D
DD
Mallya
Asha D
AD
Dhere
Rajeev M
RM
Kapre
Subhash V
SV
D000940:Antigenic Variation; D000956:Antigens, Viral / Q000235:genetics*; D019295:Computational Biology / Q000379:methods*; D049810:Genetic Speciation; D008958:Models, Molecular; D009109:Mumps virus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D011487:Protein Conformation; D013045:Species Specificity; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D000956:Antigens, Viral; D014764:Viral Proteins