Impact of neonatal imprinting with vitamin A or D on the hormone content of rat immune cells.
Neonatal single vitamin A (retinol; 3 mg) or vitamin D3 (cholecalciferol; 0.05 mg) treatment (imprinting) was done in male and female rats and 2 months later the endorphin, triiodothyronine (T3) and ACTH content of immune cells (peritoneal lymphocytes, monocyte-granulocyte-macrophage group [mo-gran], mast cells and thymic lymphocytes) were studied immunocytochemically by using flow cytometry and confocal microscopy. The T3 content was significantly decreased in peritoneal lymphocytes and in mo-gran and the endorphin content decreased in thymocytes of male animals, while ACTH was decreased in female lymphocytes and mo-gran. Vitamin D treatment was absolutely ineffective. The imprinting effects of vitamins A and D and their differences are discussed. The results call attention to the possible harmful effect of vitamin treatments during the perinatal critical period.
Csaba
György
G
Department of Genetics, Cell and Immunobiology, Semmelweis University Budapest, Hungary. csagyor@net.sote.hu
Kovács
Peter
P
Pállinger
Eva
E
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002762:Cholecalciferol / Q000008:administration & dosage / Q000494:pharmacology*; D004723:Endorphins / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006728:Hormones / Q000378:metabolism*; D007107:Immune System / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018613:Microscopy, Confocal; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014284:Triiodothyronine / Q000378:metabolism; D014801:Vitamin A / Q000008:administration & dosage / Q000494:pharmacology*; D014815:Vitamins / Q000008:administration & dosage / Q000494:pharmacology
D004723:Endorphins; D006728:Hormones; D014815:Vitamins; D014284:Triiodothyronine; D014801:Vitamin A; D002762:Cholecalciferol; D000324:Adrenocorticotropic Hormone