Membrane protein hMYADM preferentially expressed in myeloid cells is up-regulated during differentiation of stem cells and myeloid leukemia cells.
We report here the molecular cloning and characterization of a novel human gene (hMYADM) derived from a human bone marrow stromal cell (BMSC) cDNA library, which shares high homology with mouse myeloid-associated differentiation marker (MYADM). hMYADM is also closely related to many other eukaryotic proteins, which together form a novel and highly conserved MYADM-like family. hMYADM with 322-residue protein contains eight putative transmembrane segments and confocal microscopic analysis confirmed its membrane localization by using anti-hMYADM monoclonal antibody. hMYADM mRNA was selectively expressed in human monocytes, dendritic cells, promyeloid or monocytic leukemia cell lines, but not in CD4+, CD8+, CD19+ cells, nor in T cell leukemia or lymphocytic leukemia cell lines. hMYADM expression was also found in normal human bone marrow enriched for CD34+ stem cells, and the expression was up-regulated when these cells were induced to differentiate toward myeloid cells. The mRNA expression level of hMYADM significantly increased in acute promyelocytic leukemia HL-60 and chronic myelogenous leukemia K562 cell line after phorbol myristate acetate (PMA)-induced differentiation. Our study suggests that hMYADM is selectively expressed in myeloid cells, and involved in the myeloid differentiation process, indicating that hMYADM may be one useful membrane marker to monitor stem cell differentiation or myeloid leukemia differentiation.
Wang
Qingqing
Q
Institute of Immunology, Zhejiang University, 353 Yan'an Road, Hangzhou 310031, PR China.
Li
Nan
N
Wang
Xiaojian
X
Shen
Jiangen
J
Hong
Xuejun
X
Yu
Hai
H
Zhang
Yuanyuan
Y
Wan
Tao
T
Zhang
Lihuang
L
Wang
Jianli
J
Cao
Xuetao
X
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000096:biosynthesis* / Q000235:genetics; D015152:Blotting, Northern; D015153:Blotting, Western; D002417:Cattle; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D003001:Cloning, Molecular; D004285:Dogs; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D016415:Sequence Alignment; D017385:Sequence Homology; D014162:Transfection; D015854:Up-Regulation
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; C405987:MYADM protein, human; C485783:Myadm protein, mouse; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; D011506:Proteins