title: Gene mutation analysis and immunohistochemical study of beta-catenin in odontogenic tumors.
abstract: In the present study the significance of nuclear/cytoplasmic expression of beta-catenin (CTNNB1) and mutation of the CTNNB1 gene (CTNNB1) in odontogenic tumors was examined. Six ameloblastomas (five follicular ameloblastomas and one plexiform ameloblastoma) and three malignant odontogenic tumors (one metastasizing ameloblastoma, one ameloblastic carcinoma, and one primary intraosseous odontogenic carcinoma) were investigated for CTNNB1 expression and CTNNB1 mutation. Immunohistochemically, all follicular ameloblastomas and one primary intraosseous odontogenic carcinoma exhibited focal and moderate nuclear/cytoplasmic expression of CTNNB1, whereas the plexiform ameloblastoma and the remaining two malignant odontogenic tumors had entirely membranous expression. CTNNB1 mutation at codon 40 of exon 3 was found in one of the six follicular ameloblastomas. The other five follicular ameloblastomas, the plexiform ameloblastoma, and the three malignant odontogenic tumors did not show mutation in exon 3 of CTNNB1. These findings further confirmed that CTNNB1 mutation is not frequent in ameloblastoma and malignant odontogenic tumors, although the abnormality of Wnt signaling may be associated with some of these tumors.
authors.affiliation: Department of Oral Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
authors.forename: Tetsumi
authors.initials: T
authors.lastname: Miyake
authors.forename: Yukichi
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kato
authors.forename: Mio
authors.initials: M
authors.lastname: Tanaka
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Sato
authors.forename: Rieko
authors.initials: R
authors.lastname: Ijiri
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Inayama
authors.forename: Yumi
authors.initials: Y
authors.lastname: Ito
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Aoki
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Kawabe
authors.forename: Iwai
authors.initials: I
authors.lastname: Tohnai
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000564:Ameloblastoma / Q000235:genetics / Q000473:pathology; D001483:Base Sequence; D002648:Child; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009808:Odontogenic Tumors / Q000235:genetics* / Q000473:pathology*; D016133:Polymerase Chain Reaction; D051176:beta Catenin / Q000235:genetics*
chemical_list: D051176:beta Catenin