Cytotoxicity of Epiphany and Resilon with a root model.
OBJECTIVE
To record the cytotoxicity of Resilon and Epiphany (Pentron clinical technologies, Wallingford, CT, USA) using a root model.


METHODS
Thirty teeth with single roots were sectioned at the enamel-cementum junction, the root canals prepared and each root then sterilized before filling with the lateral condensation technique using one of three filling materials (n = 10 per group): Resilon and Epiphany, Sealite (Septodont, Pierre Rolland, Merignac, France) and gutta-percha, Roekoseal Automix (Coltène/Whaledent, Langenau, Germany) and gutta-percha. The roots were stored at 37 degrees C in an incubator to allow for setting of the root filling materials. The apices of the roots were dipped in 1 mL of MEM culture medium for 1, 2, 7 and 30 days renewing the medium every day. After 24 h contact between the medium and the filled roots, the medium was used to measure the cytotoxicity on mouse fibroblasts L 929 with the MTT assay that recorded the mitochondrial activity of the target cells. An additional test according to ISO 10993-5 standards was undertaken to compare Resilon and Epiphany.


RESULTS
The root model showed no statistically significant differences between the sealers at 7 and 30 days (NS). Epiphany and Resilon were the most cytotoxic materials at 1 and 2 days (P < 0.001). Unlike Epiphany, Resilon was not cytotoxic when tested according to ISO 10993-5 standards.


CONCLUSIONS
The cytotoxicity of Resilon + Epiphany, due mainly to Epiphany, decreased after 2 days to reach a level comparable with commonly used root canal sealers.
Susini
G
G
Department of Restorative Dentistry and Endodontics, Université de la Méditerranée, Marseille, France.
About
I
I
Tran-Hung
L
L
Camps
J
J
D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D016001:Confidence Intervals; D003738:Dental Cements / Q000633:toxicity; D006801:Humans; D051379:Mice; D012387:Root Canal Filling Materials / Q000633:toxicity*; D018675:Toxicity Tests; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
D003738:Dental Cements; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; C486893:epiphany sealer; C486892:resilon sealer; C465381:roeko seal; C071465:sealite