Variation in leaf physiology of Salix arctica within and across ecosystems in the High Arctic: test of a dual isotope (Delta13C and Delta18O) conceptual model.
Leaf carbon isotope discrimination (Delta(13)C) varies with the balance between net photosynthesis (A) and stomatal conductance (g ( s )). Inferences that can be made with Delta(13)C are limited, as changes could reflect variation in A and/or g ( s ). Investigators have suggested that leaf delta(18)O enrichment above source water (Delta(18)O) may enable differentiation between sources of variation in Delta(13)C, as leaf Delta(18)O varies with transpiration rate (E), which is closely correlated with g ( s ) when leaves experience similar leaf to air vapor pressure differences. We examined leaf gas exchange of Salix arctica at eight sites with similar air temperatures and relative humidities but divergent soil temperatures and soil water contents near Pituffik, Greenland (76 degrees N, 38 degrees W). We found negative correlations at the site level between g ( s ) and Delta(18)O in bulk leaf tissue (r (2) = 0.62, slope = -17.9 per thousand/mol H(2)O m(-2) s(-1), P = 0.02) and leaf alpha-cellulose (r (2) = 0.83, slope = -11.5 per thousand mol H(2)O m(-2) s(-1), P < 0.01), consistent with the notion that leaf water enrichment declines with increasing E. We also found negative correlations at the site-level between intrinsic water-use efficiency (iWUE) and Delta(13)C in bulk leaf tissue (r (2) = 0.65, slope = -0.08 per thousand/mumol CO(2) /mol H(2)O, P = 0.02) and leaf alpha-cellulose (r (2) = 0.50, slope = -0.05 per thousand/[mumol CO(2) /mol H(2)O], P = 0.05). When increasing Delta(13)C was driven by increasing g ( s ) alone, we found negative slopes between Delta(13)C and Delta(18)O for bulk leaf tissue (-0.664) and leaf alpha-cellulose (-1.135). When both g ( s ) and A (max) increased, we found steeper negative slopes between Delta(13)C and Delta(18)O for bulk leaf tissue (-2.307) and leaf alpha-cellulose (-1.296). Our results suggest that the dual isotope approach is capable of revealing the qualitative contributions of g ( s ) and A (max) to Delta(13)C at the site level. In our study, bulk leaf tissue was a better medium than leaf alpha-cellulose for application of the dual isotope approach.
Sullivan
Patrick F
PF
Environment and Natural Resources Institute, Department of Biological Sciences, University of Alaska, Anchorage, AK, 99508, USA. paddy@uaa.alaska.edu
Welker
Jeffrey M
JM
D001110:Arctic Regions; D002247:Carbon Isotopes / Q000378:metabolism; D002980:Climate; D004463:Ecology / Q000379:methods*; D017753:Ecosystem; D007553:Isotope Labeling / Q000379:methods*; D008954:Models, Biological; D010103:Oxygen Isotopes; D018515:Plant Leaves / Q000502:physiology*; D032108:Salix / Q000502:physiology*
D002247:Carbon Isotopes; D010103:Oxygen Isotopes