Preventive effect of Hochu-ekki-to on lipopolysaccharide-induced acute lung injury in BALB/c mice.
This study was designed to investigate the effect of Hochu-ekki-to (TJ-41), a Japanese herbal medicine, on the development of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. ALI was induced in female BALB/c mice by the intranasal administration of 0.1 mg/kg LPS. The mice were divided into a group receiving normal feed and another group receiving feed mixed with TJ-41 at a dose of 1 g/kg/day for 8 weeks before LPS challenge. In the bronchoalveolar lavage fluid, the preadministration of TJ-41 caused significant reduction in the absolute number of total cells, neutrophils, and macrophages. The preadministration of TJ-41 significantly inhibited increases in the serum level of keratinocyte chemoattractant (KC), which is a murine chemotaxin for neutrophils that corresponds to human interleukin-8, with respect to its concentration at 24 h after LPS challenge. Furthermore, the histopathologic findings indicated that alveolitis with leukocyte infiltration in the alveolar space was less severe in the TJ-41-treated mice than in the control mice. These findings indicated that the preadministration of TJ-41 could show an inhibitory effect on ALI in this experimental murine system associated with the suppression of chemokine production.
Tajima
Shunji
S
Division of Pulmonary Medicine, Department of Medicine, Jichi Medical School, 3311-1 Yakushiji, Shimotsuke, Tochigi, Japan. tajimash@med.niigata-u.ac.jp
Bando
Masashi
M
Yamasawa
Hideaki
H
Ohno
Shoji
S
Moriyama
Hiroshi
H
Takada
Toshinori
T
Suzuki
Eiichi
E
Gejyo
Fumitake
F
Sugiyama
Yukihiko
Y
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D018925:Chemokines / Q000096:biosynthesis; D002630:Chemotactic Factors / Q000097:blood; D002634:Chemotaxis, Leukocyte; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D015603:Keratinocytes; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000166:cytology; D012128:Respiratory Distress Syndrome / Q000188:drug therapy / Q000517:prevention & control*
D018925:Chemokines; D002630:Chemotactic Factors; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; C402943:bu-zhong-yi-qi-tang