Gene mutation analysis and immunohistochemical study of beta-catenin in odontogenic tumors.
In the present study the significance of nuclear/cytoplasmic expression of beta-catenin (CTNNB1) and mutation of the CTNNB1 gene (CTNNB1) in odontogenic tumors was examined. Six ameloblastomas (five follicular ameloblastomas and one plexiform ameloblastoma) and three malignant odontogenic tumors (one metastasizing ameloblastoma, one ameloblastic carcinoma, and one primary intraosseous odontogenic carcinoma) were investigated for CTNNB1 expression and CTNNB1 mutation. Immunohistochemically, all follicular ameloblastomas and one primary intraosseous odontogenic carcinoma exhibited focal and moderate nuclear/cytoplasmic expression of CTNNB1, whereas the plexiform ameloblastoma and the remaining two malignant odontogenic tumors had entirely membranous expression. CTNNB1 mutation at codon 40 of exon 3 was found in one of the six follicular ameloblastomas. The other five follicular ameloblastomas, the plexiform ameloblastoma, and the three malignant odontogenic tumors did not show mutation in exon 3 of CTNNB1. These findings further confirmed that CTNNB1 mutation is not frequent in ameloblastoma and malignant odontogenic tumors, although the abnormality of Wnt signaling may be associated with some of these tumors.
Miyake
Tetsumi
T
Department of Oral Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
Tanaka
Yukichi
Y
Kato
Keisuke
K
Tanaka
Mio
M
Sato
Yukiko
Y
Ijiri
Rieko
R
Inayama
Yoshiaki
Y
Ito
Yumi
Y
Aoki
Shinjiro
S
Kawabe
Ryoichi
R
Tohnai
Iwai
I
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000564:Ameloblastoma / Q000235:genetics / Q000473:pathology; D001483:Base Sequence; D002648:Child; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009808:Odontogenic Tumors / Q000235:genetics* / Q000473:pathology*; D016133:Polymerase Chain Reaction; D051176:beta Catenin / Q000235:genetics*
D051176:beta Catenin