[Establishment of genotyping method for DEL phenotype in Zhejiang Han population].
In order to establish a genotyping method for DEL phenotype in Zhejiang Han population, an AS-PCR method was developed according to the RHD 1227A allele sequence. Its specificity and sensitivity were assessed in two Rh negative populations whose RHD 1227A or DEL phenotype status was known. The results showed that in evaluation of the method by detecting 50 RHD 1227A positive and 50 RHD 1227A negative individuals, the genotyping method displayed a sensitivity of 100% and a specificity of 100%; in evaluation of the method by detecting 33 DEL positive and 89 DEL negative individuals, the sensitivity was 100%, however, there were two serologically negative samples which were confirmed as positive using genotyping method. After re-testing these two samples with serological method and sequence analysis, it was found that original serological method gave false negative results and genotyping method still showed 100% specificity. The minimal target DNA concentration of this genotyping method is 8.13 ng/microl. In conclusion, designed genotyping method can be used to identify DEL phenotype efficiently in Zhejiang Han Rh negative population.
Wu
Jun-Jie
JJ
Institute of Transfusion Medicine, Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, China. wujj2000@yahoo.com
Hong
Xiao-Zhen
XZ
Ma
Kai-Rong
KR
Xu
Xian-Guo
XG
Fu
Qi-Hua
QH
Yan
Li-Xing
LX
D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D001782:Blood Donors; D002681:China / Q000208:ethnology; D004912:Erythrocytes / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012204:Rh-Hr Blood-Group System / Q000235:genetics* / Q000276:immunology
D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen