Visible and fluorescent staining of two-dimensional gels.
Staining of two-dimensional gel constitutes a crucial step in comparative proteome analysis with respect to the number of proteins analyzed, the accuracy of spot quantification, and compatibility with mass spectrometry. The present chapter describes procedures for several visible and fluorescent dyes compatible with mass spectrometry: colloidal Coomassie blue, silver nitrate, Sypro Ruby, Deep Purple, and 5-hexadecanoylamino-fluorescein.
Chevalier
François
F
Laboratoire de Protéomique, INRA, Montpellier, France.
Rofidal
Valérie
V
Rossignol
Michel
M
D017360:Arabidopsis / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D013058:Mass Spectrometry / Q000379:methods; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods
D005456:Fluorescent Dyes; D010940:Plant Proteins