[Effect of alkaloids from celandine on calcium accumulation and oxidative phosphorylation in mitochondria depending on their DNA intercalating properties].
The effect of principal alkaloids (sanguinarine, chelerythrine, coptisine, chelidonine) of greater celandine Chelidonium majus L., as well as the alkaloids from Colchicum autumnale L. (colchicine and colchamine) on calcium accumulation and oxidative phosphorylation in rat liver mitochondria has been studied. The obtained data were compared with DNA intercalating properties of alkaloids detected by the method of thermodenaturation (DNA melting curve plots). It was found that chelerythrine and sanguinarine blocked absorption and accumulation of calcium cations and inhibited oxidative phosphorylation, while the coptisine significantly diminished those indices. Chelidonine, colchicines and colchamine had no influence on the studied characteristics. The effect of alkaloids upon mitochondria functional state correlated tightly with their DNA intercalating properties: chelerythrine and sanguinarine were strong intercalators, while coptisine was a weak one, and chelidonine, colchicine and colchamine did not interact with DNA and caused no changes in its melting point. Correlation coefficient between the intercalating properties of alkaloids and their inhibition of calcium accumulation was 0.89, and with their oxidative phosphorylation inhibition - 0.93. It is suggested that the effect of studied alkaloids upon functional properties of mitochondria can be mediated by mtDNA.
Kamins'kyĭ
V O
VO
Kryv'iak
N V
NV
Lutsyk
M D
MD
Stoĭka
R S
RS
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D028281:Chelidonium / Q000737:chemistry*; D004272:DNA, Mitochondrial / Q000378:metabolism*; D066298:In Vitro Techniques; D007364:Intercalating Agents / Q000302:isolation & purification / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D010085:Oxidative Phosphorylation / Q000187:drug effects; D051381:Rats
D000470:Alkaloids; D004272:DNA, Mitochondrial; D007364:Intercalating Agents; D002118:Calcium