Effects of growth light and nitrogen nutrition on the organization of the photosynthetic apparatus in leaves of a C4 plant, Amaranthus cruentus.
Properties of C4 photosynthesis were examined in Amaranthus cruentus L. (NAD-malic enzyme (ME) subtype, dicot) grown under different light and nitrogen (N) conditions, from the viewpoint of N investment into their photosynthetic components. In low-light (LL) leaves, chlorophyll content per leaf area was greater and chlorophyll alb ratio was lower than in high-light (HL) leaves. These indicate that LL leaves invest more N into their light-harvesting systems. However, this N investment did not contribute to the increase in the quantum yield of photosynthesis on the incident photon flux density (PFD) basis (Qi) in LL leaves. N allocation to ribulose 1,5-bisphosphate carboxylasel oxygenase (Rubisco) was significantly higher in HL-high N (HN) leaves than in other leaves. On the other hand, N allocation to C4 enzymes [phosphoenolpyruvate carboxylase (PEPC) and pyruvate Pi dikinase (PPDK)] was unaffected by the growth conditions. Maximum photosynthetic rates (Pmax) per Rubisco content were similar irrespective of the growth light treatments. Carbon isotope ratios (delta13 C) in the leaf dry matter were more negative in LL leaves than in HL leaves (LL = -19.3% per hundred, HL = -16.0% per hundred) and independent of leaf N. Vein density was highest in HL-HN leaves, and leaf thickness was unaffected by the growth light treatments. From these results, we conclude that A. cruentus leaves would not acclimate efficiently to low growth light.
Tazoe
Youshi
Y
Department of Biology, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka, 560-0043, Japan. utazoe@bio.sci.osaka-u.ac.jp
Noguchi
Ko
K
Terashima
Ichiro
I
D027721:Amaranthus / Q000378:metabolism* / Q000502:physiology / Q000528:radiation effects; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes / Q000032:analysis; D002734:Chlorophyll / Q000378:metabolism; D008027:Light; D009584:Nitrogen / Q000378:metabolism*; D010730:Phosphoenolpyruvate Carboxylase / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology / Q000528:radiation effects; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects; D018515:Plant Leaves / Q000033:anatomy & histology / Q000502:physiology / Q000528:radiation effects; D010940:Plant Proteins / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects; D011772:Pyruvate, Orthophosphate Dikinase / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism
D002247:Carbon Isotopes; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins; D002734:Chlorophyll; D002245:Carbon Dioxide; D011772:Pyruvate, Orthophosphate Dikinase; D010730:Phosphoenolpyruvate Carboxylase; D012273:Ribulose-Bisphosphate Carboxylase; D009584:Nitrogen