[Activation of RHOA gene transcription in epithelial tumors may be caused by gene amplification and/or demethylation of its promotor region].
RHOA protein, a member of small GTPases family, is implicated in cell morphogenesis, adhesion, and in cell cycle regulation. RHOA gene (3p21.31) exhibits cell transformation activity, and therefore gene is considered as a potential oncogene. The aim of this study was to investigate RHOA transcription and copy number changes in three epithelial tumors (breast, renal cell and epithelial ovarian carcinomas, 45 tumor/normal pairs altogether). EII, HhaI, AciI n Bsh1236I). Hypomethylation of the RHOA promoter region in tumor DNA was observed two times more frequently than increased methylation. Moreover, all (15) cancer cases with hypomethylation of the RHOA gene showed a 2-10 fold increased expression of RHOA. It was concluded that gene copy multiplication and demethylation of the RHOA promoter region can contribute to transcription activation of this gene in epithelial tumors.
Braga
E A
EA
Loginov
V I
VI
Klimov
E A
EA
Kilosanidze
G
G
Khodyrev
D S
DS
Kaganova
N L
NL
Kazybskaia
T P
TP
Ermilova
V D
VD
Gar'kavtseva
R F
RF
Pronina
I V
IV
Rud'ko
O I
OI
Zabarskiĭ
E R
ER
Sulimova
G E
GE
Kiselev
L L
LL
D001943:Breast Neoplasms / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000235:genetics; D019175:DNA Methylation; D005260:Female; D005784:Gene Amplification; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D020742:rhoA GTP-Binding Protein / Q000235:genetics*
D012333:RNA, Messenger; C066737:RHOA protein, human; D020742:rhoA GTP-Binding Protein