[Expression of osteopontin in the pericystic layer of hepatic hydatid cyst].
OBJECTIVE
To investigate the expression and distribution of osteopontin (OPN) in the pericystic layer of Echinococcus granulosus cyst.


METHODS
60 surgically excised cysts were studied with HE and immunohistochemistry staining by using antibodies specific for OPN. Immunofluorescence double labeling technique and Media Cybernetics Image (Pro Plus 5.1 software) were used to appraise the relationship between the OPN and macrophages (CD68).


RESULTS
Expression of OPN was found at various degree in hydatid cysts, 75% (45/60) was distributed in the intra-layer at the parasite side, 8.3% (5/60) distributed in the extra-layer near the side of liver parenchyma ("exocyst"-layer vs "adventitia"-layer, P<0.01). Macrophages were identified between the intra-layer and extra-layer, and OPN was observed in most of the macrophages. Meanwhile, OPN was concurrently found with the deposit of calcium in a similar distribution in the hydatid cyst.


CONCLUSIONS
Osteopontin mainly distributes in the intra-pericystic layer of hepatic hydatid cyst.
Li
Jian-hui
JH
Department of General Surgery, First Affiliated Hospital, Medical College of Shihezi University, Shihezi 832008, China.
Peng
Xin-yu
XY
Zhou
Zong-yao
ZY
Wu
Xiang-wei
XW
Zhang
Shi-jie
SJ
Niu
Jian-hua
JH
Sun
Hong
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002648:Child; D004444:Echinococcosis, Hepatic / Q000378:metabolism* / Q000473:pathology; D048209:Echinococcus granulosus / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000096:biosynthesis*; D011817:Rabbits
D053495:Osteopontin