title: Phylogenetic identification of Aeromonas strains isolated from carcasses of pig as new members of the species Aeromonas allosaccharophila.
abstract: The first description of the species Aeromonas allosaccharophila was only based on two strains (the type strain CECT4199, and a duplicate CECT4200) isolated from diseased elvers (Anguilla anguilla) of an eel-farm located in Valencia, Spain, and one stool isolate (ATCC35942) from a female with diarrhoea and food poisoning in South Carolina, U.S.A. In the present study, 17 Aeromonas isolates obtained from carcasses of pigs and from the equipment for the cleaning process, and one strain recently isolated from a clinical case of gastroenteritis, were genetically identified as Aerornonas allosaccharophila on the basis of gyrB and 16S rRNA gene sequencing. In addition, this phylogenetic approach also supports the classification of Aeromonas veronii biogroup sobria reference strains LMG13071, LMG13073 and LMG13074 within the species A. allosaccharophila. The A. allosaccharophila strains isolated from pig carcasses processed in a single slaughterhouse presented a clonal origin, on the basis of random amplified polymorphic DNA genetic typing. To our knowledge, this is the first time since the species description that A. allosaccharophila has been newly identified, being on this occasion isolated from the environment of a slaughterhouse. Our findings indicate that this species may be readily identified by a sequencing approach and, consequently, the present work supports the existence of this phylogenetic cluster.
authors.affiliation: Department of Veterinary Sciences, CECAV-University of Trás-os-Montes e Alto Douro, Vila Real, Portugal.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Saavedra
authors.forename: Victor
authors.initials: V
authors.lastname: Perea
authors.forename: Maria Conceiçao
authors.initials: MC
authors.lastname: Fontes
authors.forename: Conceiçao
authors.initials: C
authors.lastname: Martins
authors.forename: Antonio
authors.initials: A
authors.lastname: Martínez-Murcia
mesh_terms: D000003:Abattoirs; D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004864:Equipment and Supplies / Q000382:microbiology; D005516:Food Microbiology; D005759:Gastroenteritis / Q000382:microbiology; D020459:Genes, rRNA; D005838:Genotype; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D008460:Meat / Q000382:microbiology*; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase