[Essential genes as potential targets of antifungal agents in pathogenic yeast Candida].
An important point in the development of an antimicrobial agent is whether its target molecules are essential for growth of the microorganism. From this viewpoint, we focused attention on essential genes as potential targets of antifungal agents in the pathogenic yeast Candida. Here we introduce recent attempts for screening, identification, and characterization of essential genes from a haploid yeast Candida glabrata, using temperature-sensitive mutants. Our experimental results suggesting the essentiality of C. albicans PHO85, the homologue of which is known as a negative regulator of the PHO system and as a non-essential gene in Saccharomyces cerevisiae are also described.
Miyakawa
Yozo
Y
Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Japan.
Chibana
Hiroji
H
Uno
Jun
J
Mikami
Yuzuru
Y
Nakayama
Hironobu
H
D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects / Q000235:genetics*
D000935:Antifungal Agents