Hypothetical protein Cpn0308 is localized in the Chlamydia pneumoniae inclusion membrane.
The hypothetical protein encoded by Chlamydia pneumoniae open reading frame cpn0308 was detected in inclusion membranes of C. pneumoniae-infected cells using antibodies raised with Cpn0308 fusion proteins. The anti-Cpn0308 antibodies did not cross-react with IncA, a known C. pneumoniae inclusion membrane protein, although the anti-Cpn0308 antibody staining overlapped with the anti-IncA antibody labeling. The labeling of the inclusion membrane by the anti-Cpn0308 antibody was specifically blocked by the Cpn0308 but not IncA fusion proteins. The Cpn0308 antigen was detectable 24 h after infection and remained in the inclusion membrane throughout the infection course.
Luo
Jianhua
J
Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
Jia
Tianjun
T
Flores
Rhonda
R
Chen
Ding
D
Zhong
Guangming
G
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D016993:Chlamydophila pneumoniae / Q000502:physiology*; D006367:HeLa Cells; D006790:Host-Parasite Interactions; D006801:Humans; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D010750:Phosphoproteins / Q000378:metabolism
D001426:Bacterial Proteins; C094163:IncA protein, bacteria; D010750:Phosphoproteins