title: Specific cell behavior of human fibroblast onto carbohydrate surface detected by glycoblotting films.
abstract: We synthesized an aminooxyl polymer that is reactive with the reduced end of carbohydrates using our sugar-displaying approach. The carbohydrates were easily immobilized on the polymer film (glycoblotting film) by simple immersion in a in sugar solution through stable oxime bond. The in vitro behaviors of human fibroblasts on the carbohydrate-coated surface were investigated. The adhesion of human fibroblasts on the cellobiose- and cellotriose-coated surfaces was much greater than on the other coated surfaces and the noncoated surface. This result indicated that simple structural differences in carbohydrates induced biological changes in human cells, especially cell adhesion. Our approach provides a high-throughput assay system for carbohydrate-related cell adhesion and proliferation.
authors.affiliation: Department of Orthopaedic Surgery, Hokkaido University School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo 060-8638, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Onodera
authors.forename: Kenichi
authors.initials: K
authors.lastname: Niikura
authors.forename: Norimasa
authors.initials: N
authors.lastname: Iwasaki
authors.forename: Noriko
authors.initials: N
authors.lastname: Nagahori
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Shimaoka
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Kamitani
authors.forename: Tokifumi
authors.initials: T
authors.lastname: Majima
authors.forename: Akio
authors.initials: A
authors.lastname: Minami
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans
chemical_list: D002241:Carbohydrates