[Research on development of gold immunochromagraphic assay test kit for serum Coxsackievirus IgM antibody.].
BACKGROUND
To develop gold immunochromatographic assay (GICA) test kit for serum Coxsackievirus IgM antibody and optimize the key experiment conditions.


METHODS
Colloidal gold particles of 20 nm were prepared and coupled with sheep anti-human IgM, the gold immunochromatography assay test kit was setup for serum Coxsackievirus IgM antibody.


RESULTS
The spheroidal colloid gold particles of 20 nm showed bright red in color. The minimal stable concentration (MSC) of gold sheep anti-human IgM was 1 ug/ml, and the suitable stable concentration (SSC) was 1.5 ug/ml. The pH 8.2 was appropriate; 30 sera samples were tested by GICA and ELISA, there was no significant difference between the two methods.


CONCLUSIONS
The quality of gold immunochromatography assay test kit is associated with such factors as the quality and quantity of antigen or antibody, colloid gold particles, buffers, etc.
Zhang
B Y
BY
Capital Institute of Pediatrics, Beijing 100020, China. Corresponding author: ZHANG Bao-yuan, E-mail: drbaoyuan@163.com.
Cui
X D
XD
Ma
X H
XH
Zhang
T
T
Hu
S S
SS
D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold; D017979:Gold Colloid / Q000737:chemistry; D006801:Humans; D007075:Immunoglobulin M; D012680:Sensitivity and Specificity
D017979:Gold Colloid; D007075:Immunoglobulin M; D006046:Gold