title: Immunomodulation produced by a green propolis extract on humoral and cellular responses of mice immunized with SuHV-1.
abstract: Despite recent technological advances in vaccine production, most the vaccines depend on the association with adjuvant substances. This work evaluated the adjuvant capacity of an ethanol extract of green propolis associated to inactivated Suid herpesvirus type 1 (SuHV-1) vaccine preparations. Mice inoculated with SuHV-1 vaccine plus aluminum hydroxide and 5mg/dose of propolis extract presented higher levels of antibodies when compared to animals that received the same vaccine without propolis. The use of SuHV-1 vaccine with propolis extract alone did not induce significant levels of antibodies, however it was able to increase the cellular immune response, evidenced by the increase in the expression of mRNA to IFN-gamma. Besides, propolis increased the percentage of protected animals against challenge with a lethal dose of SuHV-1. The effect of green propolis extract on the humoral and cellular immune responses may be exploited for the development of effective vaccines.
authors.affiliation: Centro de Biotecnologia, Laboratório de Virologia e Imunologia, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), CP 354, 96010-900 Pelotas, RS, Brazil. geferson.fischer@gmail.com
authors.forename: Geferson
authors.initials: G
authors.lastname: Fischer
authors.forename: Fabricio Rochedo
authors.initials: FR
authors.lastname: Conceição
authors.forename: Fábio Pereira Leivas
authors.initials: FP
authors.lastname: Leite
authors.forename: Luana Alves
authors.initials: LA
authors.lastname: Dummer
authors.forename: Gilberto D'Avila
authors.initials: GD
authors.lastname: Vargas
authors.forename: Sílvia de Oliveira
authors.initials: Sde O
authors.lastname: Hübner
authors.forename: Odir Antônio
authors.initials: OA
authors.lastname: Dellagostin
authors.forename: Niraldo
authors.initials: N
authors.lastname: Paulino
authors.forename: Amarílis Scremin
authors.initials: AS
authors.lastname: Paulino
authors.forename: Telmo
authors.initials: T
authors.lastname: Vidor
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D004587:Electrophoresis, Agar Gel; D000431:Ethanol; D005260:Female; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007111:Immunity, Cellular / Q000187:drug effects*; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011429:Propolis / Q000494:pharmacology*; D011557:Pseudorabies / Q000276:immunology / Q000517:prevention & control; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012997:Solvents
chemical_list: D000276:Adjuvants, Immunologic; D012333:RNA, Messenger; D012997:Solvents; D000431:Ethanol; D007371:Interferon-gamma; D011429:Propolis