title: Age-related urinary excretion of BK polyomavirus by nonimmunocompromised individuals.
abstract: Two polyomaviruses, BK virus (BKV) and JC virus (JCV), are ubiquitous in the human population, generally infecting children asymptomatically and then persisting in renal tissue. It is generally thought that reactivation leads to productive infection for both viruses, with progeny shed in the urine. Several studies have shown that the rate of JC viruria increases with the age of the host, but a systematic approach to examine the shedding of BKV has not been developed. To elucidate the relationship between BK viruria and host age, we obtained urine from donors (healthy volunteers or nonimmunocompromised patients) who were divided into nine age groups, each containing 50 members. A high-sensitivity PCR was used to detect BKV and JCV DNA from urinary samples, and the specificity of amplification was confirmed by sequencing or restriction analysis of the amplified fragments. The rate of BK viruria was relatively low in subjects aged <30 years but gradually increased with age in subjects aged > or =30 years. However, BK viruria was less frequent than JC viruria in adults. The detected BKV isolates were classified into subtypes, and detection rates for individual subtypes were compared among age groups; this analysis showed that viruria of subtypes I (the most prevalent subtype) and IV (the second most prevalent subtype) occurred more frequently in older subjects. Therefore, our results reveal new aspects of BK viruria in nonimmunocompromised individuals.
authors.affiliation: Department of Urology, Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhong
authors.forename: Huai-Ying
authors.initials: HY
authors.lastname: Zheng
authors.forename: Motofumi
authors.initials: M
authors.lastname: Suzuki
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikegaya
authors.forename: Naoto
authors.initials: N
authors.lastname: Aoki
authors.forename: Shuzo
authors.initials: S
authors.lastname: Usuku
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Souichi
authors.initials: S
authors.lastname: Nukuzuma
authors.forename: Yukiharu
authors.initials: Y
authors.lastname: Yasuda
authors.forename: Noboru
authors.initials: N
authors.lastname: Kuniyoshi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yogo
authors.forename: Tadaichi
authors.initials: T
authors.lastname: Kitamura
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000652:urine*; D006801:Humans; D007121:Immunocompetence; D015994:Incidence; D007223:Infant; D007231:Infant, Newborn; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000453:epidemiology* / Q000821:virology; D017422:Sequence Analysis, DNA; D014412:Tumor Virus Infections / Q000453:epidemiology* / Q000821:virology; D017201:Virus Shedding
chemical_list: D004279:DNA, Viral