title: Protein extraction from xylem and phloem sap.
abstract: It is well known that phloem and xylem vessels transport small nutrient molecules over long distances in higher plants. The finding that proteins also occur in both transport fluids was unexpected, and the function of most of these proteins is not yet well understood. This chapter outlines how proteins can be obtained and purified from xylem and phloem saps to perform subsequent proteomic analyses.
authors.affiliation: Max-Planck-Institute of Molecular Plant Physiology, Potsdam, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Kehr
authors.forename: Martijn
authors.initials: M
authors.lastname: Rep
mesh_terms: D011232:Chemical Precipitation; D052585:Phloem / Q000033:anatomy & histology / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D052584:Xylem / Q000033:anatomy & histology / Q000737:chemistry*
chemical_list: D010940:Plant Proteins