title: Molecular cloning and functional analysis of MRLC2 differential expressed in MeishanxYorkshire F1 crossbreeds and their parents, Meishan pigs.
abstract: In order to detect the molecular basis of heterosis in pigs, suppression subtractive hybridization was carried out to investigate the difference in gene expression in the Longissimus dorsi muscle tissues between MeishanxYorkshire F1 crossbreeds and their parents, Meishan pigs. The swine myosin regulatory light chain 2 (MRLC2) gene differentially expressed between the crossbreeds and the purebreds was isolated and identified using semi-quantitative reverse transcriptase polymerase chain reaction and its complete cDNA sequence was obtained using the rapid amplification of cDNA ends method. The nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence prediction analysis reveals that the open reading frame of this gene encodes a protein of 172 amino acids containing the putative conserved domain of the EF-hand superfamily. This predicted amino acid sequence of porcine MRLC2 protein exhibits 99%, 98%, 98%, 98% and 97% identity with that of cattle, human, dog, rat and mouse, respectively. The homology analysis revealed that the MRLC2 protein was very much conserved in evolution. The tissue expression analysis indicated that the swine MRLC2 gene is highly expressed in muscle, fat, heart, liver, spleen, lung, kidney, stomach, small intestine, ovary and testis, but not expressed in pancreas.
authors.affiliation: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Xie
authors.forename: Ming-Gang
authors.initials: MG
authors.lastname: Lei
authors.forename: Yuan-Zhu
authors.initials: YZ
authors.lastname: Xiong
authors.forename: Chang-Yan
authors.initials: CY
authors.lastname: Deng
authors.forename: Si-Wen
authors.initials: SW
authors.lastname: Jiang
authors.forename: Bo
authors.initials: B
authors.lastname: Zuo
authors.forename: Feng-E
authors.initials: FE
authors.lastname: Li
authors.forename: De-Quan
authors.initials: DQ
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018994:Myosin Light Chains / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009928:Organ Specificity; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D013552:Swine / Q000235:genetics / Q000378:metabolism*
chemical_list: D018994:Myosin Light Chains