title: Expression of glucocorticoid receptor mRNAs in glucocorticoid-resistant nasal polyps.
abstract: Glucocorticoids (GCs) are the most effective group of medications available to treat inflammation. Although most patients with inflammation respond to GC, a small group of patients exhibit persistent GC-resistance with prolonged inflammation. Previously, it was proposed that the GC-resistance is caused by low amount of human GC receptor (hGRalpha) and/or excessive presence of a GC receptor isoform, hGRbeta that was generated from alternative splicing of the hGR message. We have tested this hypothesis by investigating correlation between the expression pattern of hGR mRNAs in patients with inflammatory nasal polyps and the effectiveness of GC treatment.? We have performed reverse transcription PCR analysis of mRNAs coding each hGRalpha and hGRbeta in nasal tissues.? hGRalpha mRNA was more expressed in patients with nasal polyps than in normal subjects. However, the elevated hGRalphamRNA expression was decreased after GC treatment. Compared with hGRalpha mRNA expression, level of hGRbeta mRNA expression was very low in all groups. In patients, hGRbetamRNA was expressed at a similar level regardless of GC efficacy, indicating that there is no correlation between the GC sensitivity and the expression level of hGRbeta mRNA. Thus, persistent GC-resistance is not associated with low expression of hGRa or over- expression of hGRbeta.
authors.affiliation: Department of Biotechnology, Dong-Eui University, Busan 614-714, Korea.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Choi
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Kwon
authors.forename: Soo-Jung
authors.initials: SJ
authors.lastname: Gong
authors.forename: Min-Sang
authors.initials: MS
authors.lastname: Kwon
authors.forename: Joong-Hwan
authors.initials: JH
authors.lastname: Cho
authors.forename: Jae Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Sangtaek
authors.initials: S
authors.lastname: Oh
authors.forename: Hwan-Jung
authors.initials: HJ
authors.lastname: Roh
authors.forename: Dong-Eun
authors.initials: DE
authors.lastname: Kim
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D004351:Drug Resistance; D005260:Female; D015870:Gene Expression; D005938:Glucocorticoids / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery; D012333:RNA, Messenger / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017211:Treatment Failure
chemical_list: D005938:Glucocorticoids; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; C411470:glucocorticoid receptor alpha; C121943:glucocorticoid receptor beta