N- and C-Terminal motifs in human alphaB crystallin play an important role in the recognition, selection, and solubilization of substrates.
The functions of the interactive sequences in human alphaB crystallin that are involved in chaperone activity and complex assembly of small heat shock proteins need to be characterized to understand the mechanisms of action on unfolding and misfolding proteins. Protein pin arrays identified the hydrophobic N-terminal sequence (41STSLSPFYLRPPSFLRAP58) and the polar C-terminal sequence (155PERTIPITREE165) as interactive domains in human alphaB crystallin, which were then deleted to evaluate their importance in complex assembly and chaperone activity. Size exclusion chromatography determined that the complexes formed by the deletion mutants, Delta41-58 and Delta155-165, were larger and more polydisperse than the wild-type (wt) alphaB crystallin complex. In chaperone assays, the Delta41-58 mutant was as effective as wt alphaB crystallin in protecting partially unfolded betaL crystallin and alcohol dehydrogenase (ADH) and significantly less effective than wt alphaB crystallin in protecting unfolded citrate synthase (CS) from aggregation. Chaperone activity did not correlate with complex size but corresponded with the amount of substrate protein unfolding. The results confirmed the importance of N-terminal residues 41-58 in selective interactions with completely unfolded substrates. Poor solubility and limited or no chaperone activity for the three substrates characterized the Delta155-165 deletion mutant, which demonstrated the importance of C-terminal residues 155-165 in maintaining the solubility of unfolded substrates in a manner independent of the amount of substrate protein unfolding. The results presented in this report established that interactive domains in the N- and C-termini of human alphaB crystallin are important for the recognition, selection, and solubility of unfolding substrate proteins.
Ghosh
Joy G
JG
Biomolecular Structure and Design, Department of Biological Structure, University of Washington, Seattle, Washington 98195-7420, USA.
Shenoy
Ananth K
AK
Clark
John I
JI
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D002850:Chromatography, Gel; D002942:Circular Dichroism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003459:Crystallins / Q000737:chemistry* / Q000502:physiology*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity
D003459:Crystallins; D017931:DNA Primers; D000426:Alcohol Dehydrogenase; D002950:Citrate (si)-Synthase