title: Purification and properties of NADP-dependent shikimate dehydrogenase from Gluconobacter oxydans IFO 3244 and its application to enzymatic shikimate production.
abstract: NADP-Dependent shikimate dehydrogenae (SKDH, EC 1.1.1.25) was purified from Gluconobacter oxydans IFO 3244. SKDH showed a single protein band on native-PAGE accompanying enzyme activity. It required NADP exclusively and catalyzed only the shuttle reaction between shikimate and 3-dehydroshikimate. The optimum pH for shikimate oxidation and 3-dehydroshikimate reduction was found at pH 10 and 7 respectively. SKDH proved to be a useful catalyst for shikimate production from 3-dehydroshikimate.
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan. osao@yamaguchi-u.ac.jp
authors.forename: Osao
authors.initials: O
authors.lastname: Adachi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Ano
authors.forename: Hirohide
authors.initials: H
authors.lastname: Toyama
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
mesh_terms: D000429:Alcohol Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism*; D020600:Gluconobacter oxydans / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D012765:Shikimic Acid / Q000031:analogs & derivatives / Q000378:metabolism*
chemical_list: D012765:Shikimic Acid; D009249:NADP; D000429:Alcohol Oxidoreductases; C022641:Shikimate dehydrogenase