title: After-cataract evaluation after using balanced salt solution, distilled deionized water, and 5-fluorouracil with a sealed-capsule irrigation device in the eyes of 4-week-old rabbits.
abstract: OBJECTIVE
  To evaluate the Perfect Capsule sealed-capsule irrigation device (Milvella Pty., Ltd.) using 3 substances in young rabbit eyes.
  
  
  METHODS
  St. Erik's Eye Hospital, Stockholm, Sweden.
  
  
  METHODS
  Thirty 4-week-old rabbits had clear lens extraction in both eyes. In 1 randomly selected eye, the Perfect Capsule was applied and the lens capsule was irrigated for 5 minutes with 1 of 3 substances: balanced salt solution (BSS), distilled deionized water (DDW), or 5-fluorouracil (5-FU) 50 mg/mL. In the other eye, no sealed capsule irrigation was used. Forty days postoperatively, the animals were killed and the eyes fixed in formalin for histologic analysis. After-cataract was evaluated in 3 ways: clinically, from photographs, and histologically. Central posterior capsule thickness was evaluated using a microscope, camera, and computer.
  
  
  RESULTS
  The Perfect Capsule sealed-capsule irrigation system could be used in all selected eyes. The vacuum to the anterior capsule was tight, and the system was sealed in all eyes. After-cataract developed in the BSS group and DDW group, but not in the 5-FU group. The 5-FU group had significantly less after-cataract than the other 2 groups (P<.05). There was no difference between the groups in capsule thickness.
  
  
  CONCLUSIONS
  The Perfect Capsule sealed-capsule irrigation system could be used in small eyes. Distilled deionized water did not prevent after-cataract in rabbit eyes with highly proliferative cells, but 5-FU was effective in preventing after-cataract.
authors.affiliation: St. Erik's Eye Hospital, Stockholm, Sweden.
authors.forename: Mahmoud Taie
authors.initials: MT
authors.lastname: Abdelwahab
authors.forename: Maria
authors.initials: M
authors.lastname: Kugelberg
authors.forename: Ulla
authors.initials: U
authors.lastname: Kugelberg
authors.forename: Charlotta
authors.initials: C
authors.lastname: Zetterström
mesh_terms: D000085:Acetates / Q000008:administration & dosage*; D000818:Animals; D000831:Animals, Newborn; D002386:Cataract / Q000517:prevention & control*; D049109:Cell Proliferation / Q000187:drug effects; D004338:Drug Combinations; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005472:Fluorouracil / Q000008:administration & dosage*; D007903:Lens Capsule, Crystalline / Q000187:drug effects* / Q000473:pathology; D007908:Lens, Crystalline / Q000601:surgery; D008903:Minerals / Q000008:administration & dosage*; D011183:Postoperative Complications / Q000517:prevention & control*; D011817:Rabbits; D012965:Sodium Chloride / Q000008:administration & dosage*; D007507:Therapeutic Irrigation / Q000295:instrumentation / Q000379:methods*; D014867:Water / Q000008:administration & dosage*
chemical_list: D000085:Acetates; C030660:BSS solution; D004338:Drug Combinations; D008903:Minerals; D014867:Water; D012965:Sodium Chloride; D005472:Fluorouracil