title: Value of eosinophil cationic protein and tryptase levels in bronchoalveolar lavage fluid for predicting lung function impairment in anaesthetised, asthmatic children.
abstract: Bronchial hyperactivity, a key feature of active asthma in children, is a risk factor for respiratory adverse events in the peri-operative period. The presence of activated eosinophils in the lungs and mast cell degranulation can contribute to bronchial hyperreactivity. Eosinophil cationic protein is released by activated eosinophils and tryptase reflects mast cell degranulation. This study focused on the relationship of respiratory mechanics, eosinophil cationic protein and tryptase levels in bronchoalveolar lavage fluid in asthmatic and healthy children under general anaesthesia. We measured eosinophil cationic protein and tryptase levels in bronchoalveolar lavage fluid from 21 asthmatic and 21 healthy children following induction of general anaesthesia. Respiratory system resistance and dynamic compliance were measured during mechanical ventilation. Eosinophil cationic protein was more common in bronchoalveolar lavage fluid from asthmatics (12/21) than from controls (4/21, p = 0.01) and was present at higher levels (p = 0.002). Tryptase was also more common in the asthmatics (8/21 vs 1/21, p = 0.01). Respiratory resistance was significantly higher in asthmatic children with detectable eosinophil cationic protein levels than in those with undetectable eosinophil cationic protein levels (p = 0.019). Furthermore, 50% of the asthmatics with detectable eosinophil cationic protein exhibited bronchospasm after sampling their bronchoalveolar lavage fluid. These findings suggested that high levels of eosinophil cationic protein in the bronchoalveolar lavage fluid are associated with irritable airways, presumably secondary to airway inflammation, and this might be a useful marker for respiratory adverse events in the peri-operative period.
authors.affiliation: Division of Anaesthesia, Geneva Children's Hospital, Switzerland.
authors.forename: B S
authors.initials: BS
authors.lastname: von Ungern-Sternberg
authors.forename: P D
authors.initials: PD
authors.lastname: Sly
authors.forename: R K S
authors.initials: RK
authors.lastname: Loh
authors.forename: A
authors.initials: A
authors.lastname: Isidoro
authors.forename: W
authors.initials: W
authors.lastname: Habre
mesh_terms: D000403:Airway Resistance; D000768:Anesthesia, General / Q000009:adverse effects*; D001249:Asthma / Q000503:physiopathology; D015415:Biomarkers / Q000032:analysis; D016535:Bronchial Hyperreactivity / Q000175:diagnosis* / Q000209:etiology / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002648:Child; D002675:Child, Preschool; D047150:Eosinophil Cationic Protein / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D015656:Respiratory Mechanics; D053802:Tryptases / Q000032:analysis*
chemical_list: D015415:Biomarkers; D047150:Eosinophil Cationic Protein; D053802:Tryptases