title: [In situ analysis of distribution of immunocompetent cells in tumor's local draining lymph nodes].
abstract: OBJECTIVE
  To observe the distribution of immunocompetent cells (ICCs) in tumor's local draining lymph nodes (LDLN) at different stages of disease including non-metastasis, micro-metastasis and late metastasis.
  
  
  METHODS
  71 LDLN from 22 breast cancer, 28 LDLN from 7 gastric carcinoma were analysed by using catalyzed signal amplification (CSA) immunohistochemical staining. Monoclonal antibodies (mAbs) to perforin, granzyme B, CD8, CD56, CD68, S-100, CD134 and CD25 were used to detect the amount and functional change of ICCs.
  
  
  RESULTS
  Paracortical hyperplasia and sinus histocytosis was mainly observed in tumor's LDLN. As it was reported, the density of S100(+) dendritic cells (DCs) was decreased as the draining lymph nodes became tumor-containing from the status of tumor free (P<0.05), and the morphology of DCs turned to be inactivated. As the lymph nodes were invaded by tumors, the densities of CD134(+) lymphocytes and CD25(+) cells were significantly increased (P<0.01). Furthermore, there was a significant trend of decreasing in the number of activated cytotoxic T lymphocytes (granzyme B(+) cells) in LDLN as tumor progressed from non/micro-metastasis, early metastasis to advanced stage metastasis (P<0.01). There was no obvious difference in distribution of ICC between non/micro-metastasis sentinel lymph nodes and non-sentinel lymph nodes.
  
  
  CONCLUSIONS
  The dynamic change of the ICCs in LDLN implied that immune response of tumor bearing host tends to be inhibited with the progress of tumors.
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Education Ministry, Institute for Cancer Research, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710061, China. chxl64@yahoo.com.cn
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Chen
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: He
authors.forename: Kan
authors.initials: K
authors.lastname: Chen
authors.forename: Bao-Chang
authors.initials: BC
authors.lastname: Lai
authors.forename: Lü-Sheng
authors.initials: LS
authors.lastname: Si
authors.forename: Yi-Li
authors.initials: YL
authors.lastname: Wang
mesh_terms: D002452:Cell Count; D003713:Dendritic Cells / Q000276:immunology; D004322:Drainage; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D009362:Neoplasm Metastasis / Q000276:immunology; D009367:Neoplasm Staging; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology