A rapid liquid chromatography-tandem mass spectrometry method for quantification of a biologically active molecule camboginol in the extract of Garcinia cambogia.
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of camboginol in the extract of fruit rinds of Garcinia cambogia has been developed. Separation was achieved isocratically on an RP C(18) column using a solvent system consisting of a mixture of acetonitrile-water (9:1) and methanol-acetic acid (99.5:0.5) in the ratio of 30:70 as mobile phase at a flow rate of 0.4 mL/min. A multiple reaction monitoring (MRM) method was developed for quantification of camboginol in the fruit rinds extract of G. cambogia using MRM transitions of m/z 601.4 --> m/z 176.7 and m/z 601.4 --> m/z 448.9, respectively. The calibration curve based on peak area against concentration was linear up to 50 ng/mL with a detection limit of 0.5 ng/mL. The method showed satisfactory reproducibility with a coefficient of variation of less than 6%. The method was successfully applied for quantification of camboginol in different Garcinia extracts.
Chattopadhyay
Sunil K
SK
Central Institute of Medicinal and Aromatic Plants, PO CIMAP, Lucknow-226015, India. chattsk@yahoo.com
Kumar
Satyanshu
S
D002138:Calibration; D002853:Chromatography, Liquid; D029761:Garcinia cambogia / Q000737:chemistry*; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry; D012015:Reference Standards; D012639:Seeds / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D013729:Terpenes / Q000032:analysis*; D013997:Time Factors
D013729:Terpenes; C054597:garcinol