[Development of the methods of cryopreservation and lyophilization of vaccine against duckling hepatitis virus from "K-IP" strain].
A method is developed of kryopreservation of a liquid virus vaccine against duck hepatitis virus in liquid nitrogen at -196 degrees C or in its pairs (-168 to - 175 degrees C) with the subsequent fast defreezing on water bath at 40 degrees C within 2 minutes allowing to keep to initial property of a vaccine within 2 years (the period of supervision) and the rules of vaccine lyophilization with application of three-component protective environment, with 6% of peptone, 4.8% of sorbite, 1% of gelatin with sublimation chamber temperature no less than -40 degrees C in the first 4 h of lyophilization ensuring prolongation of storage term of the a liquid vaccine from 6 months in conditions of hypothermic storage (4-8 degrees C) to one year in the dried up condition.
Stegniĭ
M Iu
MIu
D000818:Animals; D015925:Cryopreservation / Q000662:veterinary; D004355:Drug Stability; D004356:Drug Storage; D004372:Ducks / Q000821:virology*; D005612:Freeze Drying; D006518:Hepatitis Viruses / Q000276:immunology / Q000302:isolation & purification; D006524:Hepatitis, Viral, Animal / Q000517:prevention & control* / Q000821:virology; D014761:Viral Hepatitis Vaccines / Q000592:standards*
D014761:Viral Hepatitis Vaccines