title: [Effect of different post-weaning dietary compositions on body fat content and hormone-sensitive lipase gene expression in rats fed with high-fat diet].
abstract: OBJECTIVE
  To study the effects of different postweaning dietary compositions on body fat content and hormone-sensitive lipase (HSL) gene expression in rats fed a high-fat diet.
  
  
  METHODS
  Male Wistar rats weaned on 24 days were randomly divided into A, B, C, D groups and were fed on high carbohydrate, high protein, high unsaturated fatty acid and high saturated fatty acid diet respectively. After 3 weeks, all rats were assigned to basic diet for two weeks, then rats from A group were randomly divided into A1 and A2 subgroups. A1 subgroup was fed on basic diet continuously. A2 subgroup and B, C and D groups were assigned to high-fat diet for six weeks. Eight rats from each group were sacrificed at the end of different treatments to detect body weight, body fat content and blood glucose level. RT-PCR was used to determine the gene expression of hormone-sensitive lipase in white adipose tissue.
  
  
  RESULTS
  Body weight, body fat content and blood glucose level in C group were significantly lower than those in A2 subgroup on 11 weeks (P < 0.05). The body weight, body fat content in B2 group were significantly lower than those in A2 subgroup (P < 0.05), but blood glucose level was higher than A2 subgroup on 11 weeks (P > 0.05). The body weight in D group was significantly lower than that in A2 subgroup (P < 0.05), but body fat content and blood glucose level were not significantly lower than A2 subgroup on 11 weeks (P > 0.05). The expression of HSL in B2 and C group was higher on 9 weeks and 11 weeks respectively. The HSL mRNA levels in all groups on 3 weeks were similar to that on 11 weeks.
  
  
  CONCLUSIONS
  Feeding on high unsaturated-fatty acid diet after weaning may significantly reduce the body weight, body fat content, improve the abnormal blood glucose level in rats induced by high-fat diet. It was suggested that the high unsaturated-fatty acid diet can be reasonable for weaned rats and gene expression regulation may be involved in programming later obesity by early nutrition.
authors.affiliation: Medical School of Ningbo University, Ningbo 315211, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Zhang
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Wang
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Zhao
authors.forename: Chang-Hao
authors.initials: CH
authors.lastname: Sun
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001823:Body Composition; D050218:Body Fat Distribution; D001835:Body Weight; D004041:Dietary Fats / Q000008:administration & dosage*; D015870:Gene Expression; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D002787:Sterol Esterase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004041:Dietary Fats; D002787:Sterol Esterase