Quantitation of trans-resveratrol and detection of its metabolites in human plasma and urine by high performance liquid chromatography.
We describe a reversed-phase HPLC method that uses gradient elution and UV detection (325 nm) to determine levels of resveratrol and identify six major conjugated metabolites in the plasma and urine of human volunteers after administration of a single oral dose of 1g. Waters Atlantis C18 3 microm served as the stationary phase. The gradient was formed using ammonium acetate and methanol, containing 2% propan-2-ol. Detection is linear between 5 ng/mL and 500 ng/mL in plasma (5-1000 ng/mL in urine). The coefficient of variation for intra- and inter-day variation is <10%. The average recovery of resveratrol from plasma and urine is 58+/-3%. The data presented in this report demonstrate a rapid, sensitive and accurate method for the analysis of resveratrol and its metabolites in human plasma and urine for pharmacokinetic studies.
Boocock
David J
DJ
Department of Cancer Studies & Molecular Medicine, Cancer Biomarkers & Prevention Group, 5th Floor RKCSB, Leicester Royal Infirmary, Leicester LE2 7LX, UK. djb27@le.ac.uk
Patel
Ketan R
KR
Faust
Guy E S
GE
Normolle
Daniel P
DP
Marczylo
Timothy H
TH
Crowell
James A
JA
Brenner
Dean E
DE
Booth
Tristan D
TD
Gescher
Andreas
A
Steward
William P
WP
D020533:Angiogenesis Inhibitors / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D000077185:Resveratrol; D013056:Spectrophotometry, Ultraviolet; D013267:Stilbenes / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*
D020533:Angiogenesis Inhibitors; D013267:Stilbenes; D000077185:Resveratrol