Identification of p38MAPK-dependent genes with changed transcript abundance in H2O2-induced premature senescence of IMR-90 hTERT human fibroblasts.
Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H2O2. We showed herein that p38(MAPK) was phosphorylated after exposure of IMR-90 hTERT cells to H2O2. Selective inhibition of p38(MAPK) activity attenuated the increase in the proportion of cells positive for senescence associated beta-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38(MAPK) inhibitor and p38(MAPK) small interferent RNA, we identified several p38(MAPK)-target genes differentially expressed in H2O2-stressed IMR-90 hTERT fibroblasts.
Zdanov
Stéphanie
S
Research Unit on Cellular Biology (URBC), University of Namur (FUNDP), Rue de Bruxelles, 61 B-5000 Namur, Belgium.
Debacq-Chainiaux
Florence
F
Remacle
José
J
Toussaint
Olivier
O
D002460:Cell Line; D016922:Cellular Senescence / Q000187:drug effects* / Q000235:genetics; D005347:Fibroblasts / Q000201:enzymology*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016877:Oxidants / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics; D019098:Telomerase / Q000096:biosynthesis* / Q000235:genetics; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
D016877:Oxidants; D047428:Protein Kinase Inhibitors; D034741:RNA, Small Interfering; D006861:Hydrogen Peroxide; D048051:p38 Mitogen-Activated Protein Kinases; D019098:Telomerase