title: Neuroglobin and cytoglobin overexpression protects human SH-SY5Y neuroblastoma cells against oxidative stress-induced cell death.
abstract: Although reactive oxygen species (ROS) at physiological concentrations are required for normal cell function, excessive production of ROS is detrimental to cells. Neuroglobin and cytoglobin are two globins, whose functions are still a matter of debate. A potential role in the detoxification of ROS is suggested. The influence of neuroglobin and cytoglobin on cell death after oxidative stress in human neuroblastoma SH-SY5Y cells was evaluated. Exposure of SH-SY5Y cells to paraquat or H(2)O(2) resulted in a concentration- and time-dependent induction of apoptotic and necrotic cell death. H(2)O(2) was 16 times more potent to induce cell death as compared to paraquat. SH-SY5Y cells transfected with plasmid DNA containing the neuroglobin or cytoglobin sequence showed enhanced survival after exposure to 300 microM H(2)O(2) for 24h as compared to untransfected controls. This finding suggests that neuroglobin and cytoglobin protect SH-SY5Y cells against oxidative stress-induced cell death.
authors.affiliation: Department of Biomedical Sciences, University of Antwerp, Belgium.
authors.forename: Elke
authors.initials: E
authors.lastname: Fordel
authors.forename: Liesbet
authors.initials: L
authors.lastname: Thijs
authors.forename: Wim
authors.initials: W
authors.lastname: Martinet
authors.forename: Marc
authors.initials: M
authors.lastname: Lenjou
authors.forename: Tilmann
authors.initials: T
authors.lastname: Laufs
authors.forename: Dirk
authors.initials: D
authors.lastname: Van Bockstaele
authors.forename: Luc
authors.initials: L
authors.lastname: Moens
authors.forename: Sylvia
authors.initials: S
authors.lastname: Dewilde
mesh_terms: D015153:Blotting, Western / Q000379:methods; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D000078842:Cytoglobin; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000379:methods; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology*; D005914:Globins / Q000378:metabolism*; D006540:Herbicides / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009447:Neuroblastoma; D000078787:Neuroglobin; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010269:Paraquat / Q000494:pharmacology; D013997:Time Factors
chemical_list: C000627265:CYGB protein, human; D000078842:Cytoglobin; D006540:Herbicides; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D016877:Oxidants; D005914:Globins; D006861:Hydrogen Peroxide; D010269:Paraquat