title: Application of an in-line imprinted polymer column in a potentiometric flow-injection chemical sensor to the determination of the carbamate pesticide carbaryl in complex biological matrices.
abstract: A flow-injection biosensor-like system based on a nonenzymatic approach has been developed to determine the carbamate pesticide carbaryl in complex biological samples without lengthy and expensive extraction steps. Molecularly imprinted polymeric beads were used to immobilize carbaryl from biological samples. pH variation permitted the elution of carbaryl from the binding cavity to the flow cell. A pH electrode was used to detect changes in the charge of carbaryl in the sample solution resulting from the protonation and deprotonation of the molecule over different pH ranges. At pH 2.0, the secondary amine group is protonated, giving a (+1) charge to the carbaryl molecule. At pH 8.0, the ionized carbaryl loses a proton to become neutral, changing the local pH of the flow cell. The pH change at the flow cell generated by the deprotonation of carbaryl ion in alkaline medium was used to determine the carbaryl concentration. Parameters influencing the performance of the system were optimized for use in the detection procedure. The validated biosensor-like system had a carbaryl detection limit of 10.0 microg/mL and a response that was linear (r2>0.98) over the concentration range of 10.0-00 microg/mL.
authors.affiliation: Charles River Laboratories Preclinical Services Montreal, Inc., 87 Senneville Road, Senneville, Québec, H9X 3R3, Canada. hanatash@hotmail.com
authors.forename: Jamil
authors.initials: J
authors.lastname: Hantash
authors.forename: Alan
authors.initials: A
authors.lastname: Bartlett
authors.forename: Philip
authors.initials: P
authors.lastname: Oldfield
authors.forename: Georges
authors.initials: G
authors.lastname: Dénès
authors.forename: Roger
authors.initials: R
authors.lastname: O'Rielly
authors.forename: Francois
authors.initials: F
authors.lastname: David
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D002021:Buffers; D002138:Calibration; D012721:Carbaryl / Q000032:analysis* / Q000097:blood; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011199:Potentiometry / Q000379:methods; D051381:Rats; D015203:Reproducibility of Results
chemical_list: D002021:Buffers; D011108:Polymers; D012721:Carbaryl