Genetic diversity of SIRE-1 retroelements in annual and perennial Glycine species revealed using SSAP.
Sequence Specific Amplification Polymorphisms (SSAP) were used to measure the distribution and structure of SIRE-1 retroelement populations in annual and perennial Glycine species. For SSAP analysis, primers corresponding to a region immediately upstream of the 3'LTR of the soybean retroelement SIRE-1 were chosen. Analysis reveals that SIRE-1 is present throughout the Glycine genus and shows that the annual species have similar SIRE-1 populations whilst the perennial species have much more distinct and diverse populations. The high number of species-specific subgroups suggest that SIRE-1 has been active and evolving independently in each species during the course of Glycine evolution.
Chesnay
Catherine
C
Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Kumar
Amar
A
Pearce
Stephen R
SR
D019143:Evolution, Molecular; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D018626:Retroelements / Q000235:genetics*; D013025:Glycine max / Q000235:genetics*
D018626:Retroelements