title: Crystallization of Ranasmurfin, a blue-coloured protein from Polypedates leucomystax.
abstract: Ranasmurfin, a previously uncharacterized approximately 13 kDa blue protein found in the nests of the frog Polypedates leucomystax, has been purified and crystallized. The crystals are an intense blue colour and diffract to 1.51 A with P2(1) symmetry and unit-cell parameters a = 40.9, b = 59.9, c = 45.0 A, beta = 93.3 degrees . Self-rotation function analysis indicates the presence of a dimer in the asymmetric unit. Biochemical data suggest that the blue colour of the protein is related to dimer formation. Sequence data for the protein are incomplete, but thus far have identified no model for molecular replacement. A fluorescence scan shows a peak at 9.676 keV, indicating that the protein binds zinc and suggesting a route for structure solution.
authors.affiliation: Centre for Biomolecular Science and The Scottish Structural Proteomics Facility, The University of St Andrews Fife KY16 9RH, Scotland, UK.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: McMahon
authors.forename: Martin A
authors.initials: MA
authors.lastname: Walsh
authors.forename: Rosalind Tan Yan
authors.initials: RT
authors.lastname: Ching
authors.forename: Lester G
authors.initials: LG
authors.lastname: Carter
authors.forename: Mark
authors.initials: M
authors.lastname: Dorward
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Johnson
authors.forename: Huanting
authors.initials: H
authors.lastname: Liu
authors.forename: Muse
authors.initials: M
authors.lastname: Oke
authors.forename: Carlos
authors.initials: C
authors.lastname: Bloch
authors.forename: Malcolm W
authors.initials: MW
authors.lastname: Kennedy
authors.forename: Aishah A
authors.initials: AA
authors.lastname: Latiff
authors.forename: Alan
authors.initials: A
authors.lastname: Cooper
authors.forename: Garry L
authors.initials: GL
authors.lastname: Taylor
authors.forename: Malcolm F
authors.initials: MF
authors.lastname: White
authors.forename: James H
authors.initials: JH
authors.lastname: Naismith
mesh_terms: D000818:Animals; D001001:Anura; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation
chemical_list: D002352:Carrier Proteins