Gene stage-specific expression in the microenvironment of pediatric myelodysplastic syndromes.
Using cDNA microarray assays we have observed a clear difference in the gene expression pattern between bone marrow stromal cells obtained from healthy children (CT) and from pediatric patients with either myelodysplastic syndromes (MDS) or acute myeloid leukemia (AML) associated with MDS (MDS-AML). The global gene function profiling analysis indicated that in the pediatric MDS microenvironment the disease stages may be characterized mainly by underexpression of genes associated with biological processes such as transport. Furthermore, a subset of downregulated genes related to endocytosis and protein secretion was able to discriminate MDS from MDS-AML.
Roela
Rosimeire A
RA
Faculdade de Medicina da Universidade de São Paulo, Departamento de Radiologia (LIM24), Av Dr Arnaldo 455 sala 4112, São Paulo 01246-903, Brazil.
Carraro
Dirce M
DM
Brentani
Helena P
HP
Kaiano
Jane H L
JH
Simão
Daniel F
DF
Guarnieiro
Roberto
R
Lopes
Luiz Fernando
LF
Borojevic
Radovan
R
Brentani
M Mitzi
MM
D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D007223:Infant; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000473:pathology; D008297:Male; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
D012333:RNA, Messenger