[Fluorescein angiography of retinal neovascularization model in mice].
OBJECTIVE
To investigate a high molecular weight fluorescein angiography in the application of retinal neovascularization model in mouse.


METHODS
retinal neovascularization model was induced by exposure mouse to an environment containing high concentration of oxygen. High molecular weight fluorescein isothiocyanate dextran were perfused through the left ventricle directly, then the mouse eyes were enucleated and fixed with 4% paraformaldehyde. The retina was separated from the eyecup and flat mounting was performed on a gelatin coated slide. The vasculature was examined under fluorescent microscope.


RESULTS
The whole retinal vasculature was clearly visualized under fluorescent microscope. By focusing on different layer of the tissue, superficial, deep vascular plexus and connecting vessels also could be distinguished. The neovascular response occurred at the junction between the vascular and the avascular retina.


CONCLUSIONS
High molecular weight fluorescein angiography can be applied for retinal neovascularization evaluation.
Kong
Yi-chun
YC
Tianjin Medical University, Tianjin Eye Hospital, Tianjin 300022, China.
Han
Mei
M
Zhao
Kan-xing
KX
Li
Ning-dong
ND
Lin
Jin-yong
JY
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D019793:Fluorescein; D005451:Fluorescein Angiography / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000633:toxicity; D015861:Retinal Neovascularization / Q000175:diagnosis*
D010100:Oxygen; D019793:Fluorescein