title: Glycerol nucleoside triphosphates: synthesis and polymerase substrate activities.
abstract: [Structure: see text] The synthesis of (S)-glycerol nucleoside triphosphates (gNTPs) and the analysis of their substrate activities for enzymatic polymerization is described. NTPs with simplified carbohydrate backbones such as the tNTPs (alpha-L-threose-NTPs) are polymerase substrates and offer the potential to create non-natural aptamer sequences with simplified backbones through enzymatic means. The acyclic (S)-GNA was modeled after the shortened alpha-threofuranosyl backbone. Here we describe the synthesis of (S)-glycerol NTPs and initial enzymatic testing of this further simplified nucleic acid backbone.
authors.affiliation: Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, and Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
authors.forename: Allen T
authors.initials: AT
authors.lastname: Horhota
authors.forename: Jack W
authors.initials: JW
authors.lastname: Szostak
authors.forename: Larry W
authors.initials: LW
authors.lastname: McLaughlin
mesh_terms: D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005990:Glycerol / Q000737:chemistry*; D015394:Molecular Structure; D009705:Nucleosides / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D009705:Nucleosides; D004259:DNA-Directed DNA Polymerase; D005990:Glycerol