Plasma creatinine determination in mice and rats: an enzymatic method compares favorably with a high-performance liquid chromatography assay.
The use of the colorimetric Jaffé method for the measurement of creatinine in mouse and rat plasma has been criticized as prior studies have shown a dramatic overestimation. We compared a colorimetric picric acid, an enzymatic, and a high-performance liquid chromatography (HPLC) method to assess their appropriateness for routine measurements of creatinine in plasma of healthy and diseased mice (n=61) and rats (n=56). For the colorimetric Jaffé method a pronounced overestimation is confirmed. Additionally the method showed interference with hemoglobin already in a very low, non-visible concentration range in rat plasma. The enzymatic measurement demonstrated a hemoglobin interference in mice, only when hemolysis was visible. The comparison between HPLC and the enzymatic measurement gave a good agreement between both methods in both species. Therefore the enzymatic method fulfills the requirements for a routine screening test for plasma creatinine in healthy as well as diseased mice and rats Kiover a broad concentration range.
Keppler
A
A
Medical Research Center, University of Heidelberg, Theodor-Kutzer-Ufer, Mannheim, Germany.
Gretz
N
N
Schmidt
R
R
Kloetzer
H-M
HM
Groene
H-J
HJ
Lelongt
B
B
Meyer
M
M
Sadick
M
M
Pill
J
J
D000581:Amidohydrolases; D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D003404:Creatinine / Q000097:blood*; D006461:Hemolysis; D051379:Mice; D008817:Mice, Mutant Strains; D010851:Picrates; D007690:Polycystic Kidney Diseases / Q000097:blood / Q000235:genetics; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D014512:Ureohydrolases
D010851:Picrates; C005858:picric acid; D003404:Creatinine; D000581:Amidohydrolases; C019946:creatininase; D014512:Ureohydrolases; C019944:creatinase