Cloning, genomic organisation and mRNA expression of a pectin lyase gene from a mutant strain of Penicillium occitanis.
The regulatory cis elements of fungal pectinases are well studied in Aspergillus genera but little is known in other fungal species. A genomic bank from Penicillium occitanis fungus is constructed and screened by previously isolated cDNA probe of a pectin lyase. From several isolated clones, the nucleotide sequence of the pectin lyase gene was completed and led to the identification of introns and promoter-terminator regions. A streaking future was found in pnl gene of P. occitanis: it exhibits the highest nucleotide homology with the pnlA of Aspergillus niger but the positions of its 4 introns is completely identical to that of A. niger pnlB gene. In addition to the determination of transcription start site, the promoter sequence from the pnl gene was analysed. It showed the conservation of known consensus sequences -CreA, Hap2-3-4, PacC ...-, and the existence of a particular sequence -CCTGA- which is similar to that already found to be specific of pectinolytic gene in Aspergillus, CCCTGA. This result suggests that the corresponding regulatory trans-acting factor should be the same as in Aspergillus.
Trigui-Lahiani
Hèla
H
Laboratoire de Génétique Moléculaire des Eucaryotes, Centre de Biotechnologie de Sfax, BP K 3038-Sfax, Tunisia.
Gargouri
Ali
A
D024509:3' Flanking Region; D024506:5' Flanking Region; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003062:Codon; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D015698:Genomic Library; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D010407:Penicillium / Q000201:enzymology / Q000235:genetics*; D010802:Phylogeny; D011133:Polysaccharide-Lyases / Q000235:genetics* / Q000378:metabolism; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D024363:Transcription Initiation Site
D003062:Codon; D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger; D011133:Polysaccharide-Lyases; C027349:pectin lyase