title: Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization.
abstract: The entire gene cluster involved in the biosynthesis of angucyclines Sch 47554 and Sch 47555 was cloned, sequenced, and characterized. Analysis of the nucleotide sequence of genomic DNA spanning 77.5-kb revealed a total of 55 open reading frames, and the deduced products exhibited strong sequence similarities to type II polyketide synthases, deoxysugar biosynthetic enzymes, and a variety of accessory enzymes. The involvement of this gene cluster in the pathway of Sch 47554 and Sch 47555 was confirmed by genetic inactivation of the aromatase, including a portion of the ketoreductase, which was disrupted by inserting the thiostrepton gene.
authors.affiliation: Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University, Asan 336-708, Korea.
authors.forename: Devi Bahdur
authors.initials: DB
authors.lastname: Basnet
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Oh
authors.forename: Thi Thu Hang
authors.initials: TT
authors.lastname: Vu
authors.forename: Basundhara
authors.initials: B
authors.lastname: Sthapit
authors.forename: Kwangkyoung
authors.initials: K
authors.lastname: Liou
authors.forename: Hei Chan
authors.initials: HC
authors.lastname: Lee
authors.forename: Jin-Cheol
authors.initials: JC
authors.lastname: Yoo
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Sohng
mesh_terms: D001483:Base Sequence; D001551:Benz(a)Anthracenes / Q000737:chemistry / Q000378:metabolism; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D048630:Polyketide Synthases / Q000235:genetics; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001551:Benz(a)Anthracenes; C081748:Sch 47554; C081749:Sch 47555; D048630:Polyketide Synthases