title: Hypothetical protein Cpn0308 is localized in the Chlamydia pneumoniae inclusion membrane.
abstract: The hypothetical protein encoded by Chlamydia pneumoniae open reading frame cpn0308 was detected in inclusion membranes of C. pneumoniae-infected cells using antibodies raised with Cpn0308 fusion proteins. The anti-Cpn0308 antibodies did not cross-react with IncA, a known C. pneumoniae inclusion membrane protein, although the anti-Cpn0308 antibody staining overlapped with the anti-IncA antibody labeling. The labeling of the inclusion membrane by the anti-Cpn0308 antibody was specifically blocked by the Cpn0308 but not IncA fusion proteins. The Cpn0308 antigen was detectable 24 h after infection and remained in the inclusion membrane throughout the infection course.
authors.affiliation: Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Luo
authors.forename: Tianjun
authors.initials: T
authors.lastname: Jia
authors.forename: Rhonda
authors.initials: R
authors.lastname: Flores
authors.forename: Ding
authors.initials: D
authors.lastname: Chen
authors.forename: Guangming
authors.initials: G
authors.lastname: Zhong
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D016993:Chlamydophila pneumoniae / Q000502:physiology*; D006367:HeLa Cells; D006790:Host-Parasite Interactions; D006801:Humans; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D010750:Phosphoproteins / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C094163:IncA protein, bacteria; D010750:Phosphoproteins