[Advances in plant proteomics--I. Key techniques of proteome].
With the completion of genome sequences of the model plants, such as rice (Oryza sativa L.) and Arabidopsis thaliana, it has come into plant functional genomics era, which becomes a hard base of the appearance and development of plant proteomics. This review focuses on the background of proteomics, concept of proteomics and key techniques of proteomics. The key techniques of proteomics include separation, such as 2-DE (Two-Dimensional Electrophoresis), RP-HPLC (Reverse Phase High Performance Liquid Chromatography) and SELDI (Surface Enhanced Laser Desorption/ Ionization) protein chip, mass spectrometry, such as MALDI-TOF-MS (Matrix Assisted Laser Desorption/Ionization-Time Of Flight-Mass Spectrometry) and ESI-MS/MS (Electrospray Ionization Mass Spectrometry/Mass Spectrometry), databases related to proteomics, quantitative proteome, TAP (Tandem Affinity Purification) and yeast two-hybrid system. Challenges and prospects of proteomic techniques are discussed.
Ruan
Song-Lin
SL
Plant Molecular Biology & Proteomics Lab, Institute of Biotechnology Hangzhou Academy of Agriculture Science, Hangzhou 310024, China. ruansl@hotmail.com
Ma
Hua-Sheng
HS
Wan
Shi-Heng
SH
Xin
Ya
Y
Qian
Li-Hua
LH
Tong
Jian-Xing
JX
Wang
Jie
J
D019295:Computational Biology; D030562:Databases, Protein; D010940:Plant Proteins / Q000032:analysis* / Q000302:isolation & purification; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*
D010940:Plant Proteins; D020543:Proteome