title: Engineering of human tracheal tissue with collagen-enforced poly-lactic-glycolic acid non-woven mesh: a preliminary study in nude mice.
abstract: The purpose of the current study is to fabricate tissue engineered trachea with poly-lactic-glycolic acid (PLGA) non-woven mesh enforced by collagen type I. PLGA fibres coated with collagen solution were put together and fabricated into the shape of a human trachea, after drying and cross-linking treatment, a non-woven mesh with "C" shape formed. Chondrocytes from sheep nasal septum cartilage were expanded in vitro and seeded into PLGA/collagen non-woven mesh in the density of 5.0 x 10(7)mL(-1). After 5 days of in vitro incubation, six Cell-PLGA/collagen composites were implanted subcutaneously into the back of 6 nude mice to prefabricate a tissue engineering trachea. Eight weeks later, the cartilage formation was observed by gross inspection and histological examination. Cartilage-like tissue in the shape of the initial PLGA/collagen scaffold had been regenerated successfully without obvious inflammatory response. The tissue engineered trachea cartilage consisted of evenly spaced lacunae embedded in matrix stained red with safranin-O staining. The amount of GAGs in tissue engineered trachea cartilage reached 71.42% of normal value in native cartilage. This study demonstrated that collagen-enforced PLGA non-woven mesh facilitated the adhesion and proliferation of chondrocytes, it also owned adequate mechanical strength to serve as an ideal scaffold for trachea tissue engineering without internal support.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Qindu Hospital, The Fourth Military Medical University, Xi'an, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Xue
authors.initials: X
authors.lastname: Feng
authors.forename: Tianqiu
authors.initials: T
authors.lastname: Mao
authors.forename: Xinghua
authors.initials: X
authors.lastname: Feng
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Ouyang
authors.forename: Guifang
authors.initials: G
authors.lastname: Zhao
authors.forename: Fulin
authors.initials: F
authors.lastname: Chen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002356:Cartilage / Q000033:anatomy & histology; D002448:Cell Adhesion; D002452:Cell Count; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D020219:Chondrogenesis / Q000502:physiology; D024042:Collagen Type I / Q000737:chemistry; D004396:Coloring Agents; D003432:Cross-Linking Reagents / Q000737:chemistry; D003890:Desiccation; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D051379:Mice; D008819:Mice, Nude; D009300:Nasal Septum / Q000166:cytology; D010619:Phenazines; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D012756:Sheep; D040521:Subcutaneous Tissue / Q000601:surgery; D013526:Surgical Mesh; D023822:Tissue Engineering / Q000379:methods; D014132:Trachea / Q000033:anatomy & histology*
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D004396:Coloring Agents; D003432:Cross-Linking Reagents; D006025:Glycosaminoglycans; D010619:Phenazines; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C009195:safranine T