Analysis of microsatellite instability in stool DNA of patients with colorectal cancer using denaturing high performance liquid chromatography.
OBJECTIVE
To evaluate the usefulness of denaturing high performance liquid chromatography (DHPLC) for analyzing microsatellite instability (MSI) status in stool DNA of patients with colorectal cancer.


METHODS
A total of 80 cancer tissues from patients with primary sporadic colorectal tumor (proximal cancer: 27, distal cancer: 53) and matched stool (which were employed for comparison with the tissues) were analyzed for MSI status in BAT 26. DNA samples extracted from stool were evaluated by nested polymerase chain reaction (PCR) and DHPLC for MSI analysis.


RESULTS
Six cases (7.5%) of MSI were identified in BAT 26 from 80 cancer tissues. All the stool DNA samples from patients whose cancer tissue showed MSI also displayed MSI in BAT 26.


CONCLUSIONS
As MSI is one of the established fecal DNA markers to screen colorectal cancer, we propose to use DHPLC for the MSI analysis in fecal DNA.
Lim
Seok-Byung
SB
Research Institute and Hospital, National Cancer Center, Goyang, and Cancer Research Institute and Cancer Research Center, Seoul National University, Seoul, Korea.
Jeong
Seung-Yong
SY
Kim
Il-Jin
IJ
Kim
Dae Yong
DY
Jung
Kyung Hae
KH
Chang
Hee Jin
HJ
Choi
Hyo Seong
HS
Sohn
Dae Kyung
DK
Kang
Hio Chung
HC
Shin
Yong
Y
Jang
Sang-Geun
SG
Park
Jae-Hyun
JH
Park
Jae-Gahb
JG
D014408:Biomarkers, Tumor / Q000235:genetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015179:Colorectal Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D005243:Feces / Q000737:chemistry; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D053842:Microsatellite Instability
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm