title: SEC14-like protein 1 interacts with cholinergic transporters.
abstract: Trafficking of the vesicular acetylcholine transporter (VAChT) to synaptic vesicles has the potential to regulate storage and release of acetylcholine. We used the C-terminal tail of the vesicular acetylcholine transporter as bait for the screening of a brain cDNA library by yeast-two hybrids. Here we report an interaction uncovered in this screening with SEC14L1, a mammalian SEC14-like protein that may function as a phospholipid transfer protein. The interaction of VAChT and SEC14L1 occurred through the GOLD domain found in the latter and was confirmed in mammalian cells. In addition, we also found that SEC14L1 co-immunoprecipitates with the high affinity choline transporter (CHT1), but not with synaptophysin or synaptotagmin. In cultured cells SEC14L1 was predominantly found in the cytosol with little or no localization in defined organelles. In contrast, overexpression of VAChT or CHT1 with SEC14L1 recruited the latter to large intracellular organelles similar to vesicles or vesicle aggregates. Finally, we find that overexpression of SEC14L1 modestly decreases high affinity choline transport activity. We suggest that interaction of cholinergic transporters with proteins containing the GOLD domain may be relevant for transporter function.
authors.affiliation: Departamento de Bioquímica-Imunologia, Brazil.
authors.forename: Fabíola M
authors.initials: FM
authors.lastname: Ribeiro
authors.forename: Lucimar T
authors.initials: LT
authors.lastname: Ferreira
authors.forename: Sebastian
authors.initials: S
authors.lastname: Marion
authors.forename: Stefany
authors.initials: S
authors.lastname: Fontes
authors.forename: Marcus
authors.initials: M
authors.lastname: Gomez
authors.forename: Stephen S G
authors.initials: SS
authors.lastname: Ferguson
authors.forename: Marco A M
authors.initials: MA
authors.lastname: Prado
authors.forename: Vania F
authors.initials: VF
authors.lastname: Prado
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry / Q000235:genetics; D002352:Carrier Proteins / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D003001:Cloning, Molecular; D003600:Cytosol / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015723:Gene Library; D006801:Humans; D047468:Immunoprecipitation; D008074:Lipoproteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D016716:PC12 Cells; D049294:Phospholipid Transfer Proteins / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D009435:Synaptic Transmission / Q000502:physiology; D016708:Synaptophysin / Q000378:metabolism; D050857:Synaptotagmins / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism*; D014162:Transfection; D050494:Vesicular Acetylcholine Transport Proteins / Q000378:metabolism*
chemical_list: C501512:CHT1 protein, rat; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D018076:DNA, Complementary; D008074:Lipoproteins; D049294:Phospholipid Transfer Proteins; C493469:Pitpna protein, rat; C486622:Sec14l2 protein, rat; D016708:Synaptophysin; D015534:Trans-Activators; D050494:Vesicular Acetylcholine Transport Proteins; D050857:Synaptotagmins; D002794:Choline