title: The acute effects of microcystin LR on the transcription of nine glutathione S-transferase genes in common carp Cyprinus carpio L.
abstract: The glutathione S-transferases play important roles in the detoxification of microcystin. In this experiment, nine glutathione S-transferase genes including cytosolic GSTs (rho, mu, theta, alpha and pi), mitochondrial GST (kappa) and microsomal GSTs (mGST1, mGST2 and mGST3) were cloned from common carp Cyprinus carpio. The mRNA abundance of each carp GST isoform in liver was analyzed by real time PCR. The relative changes after stimulation with microcystin LR were also analyzed: increased levels of transcription of GST alpha, rho and mGST3 isoforms were detected at 6h post stimulation; the transcription of mu, theta and mGST2 isoforms were relatively stable; and all the GST isoforms except GST kappa and rho recovered to original levels compared with controls at 72 h. It is suggested that MC-LR showed different effects on the transcription of nine carp GST isoforms.
authors.affiliation: Donghu Experimental Station of Lake Ecosystems, State Key Laboratory for Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Fu
authors.forename: Ping
authors.initials: P
authors.lastname: Xie
mesh_terms: D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000502:physiology*; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000633:toxicity; D005982:Glutathione Transferase / Q000032:analysis / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008387:Marine Toxins / Q000633:toxicity*; D051379:Mice; D052998:Microcystins / Q000633:toxicity*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D017931:DNA Primers; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D008387:Marine Toxins; D052998:Microcystins; D012333:RNA, Messenger; D005982:Glutathione Transferase; C057862:cyanoginosin LR