title: Identification and quantification of methanogenic Archaea in adult chicken ceca.
abstract: By using molecular methods for the identification and quantification of methanogenic archaea in adult chicken ceca, 16S rRNA genes of 11 different phylotypes, 10 of which were 99% similar to Methanobrevibacter woesei, were found. Methanogen populations, as assessed by cultivation, and the 16S rRNA copy number were between 6.38 and 8.23 cells/g (wet weight) and 5.50 and 7.19 log(10)/g (wet weight), respectively.
authors.affiliation: University of Arkansas, Department of Food Science, 2650 N. Young Avenue, Fayetteville, AR 72704-5690, USA.
authors.forename: Suwat
authors.initials: S
authors.lastname: Saengkerdsub
authors.forename: Robin C
authors.initials: RC
authors.lastname: Anderson
authors.forename: Heather H
authors.initials: HH
authors.lastname: Wilkinson
authors.forename: Woo-Kyun
authors.initials: WK
authors.lastname: Kim
authors.forename: David J
authors.initials: DJ
authors.lastname: Nisbet
authors.forename: Steven C
authors.initials: SC
authors.lastname: Ricke
mesh_terms: D000818:Animals; D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002432:Cecum / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D019641:DNA, Archaeal / Q000032:analysis; D005260:Female; D008697:Methane / Q000378:metabolism*; D041581:Methanobrevibacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S; D008697:Methane