title: Evidence of vascular damage in dengue disease: demonstration of high levels of soluble cell adhesion molecules and circulating endothelial cells.
abstract: Clinical evidence suggests that vascular damage plays a key role in the pathophysiology of dengue hemorrhagic fever (DHF). In this study, the authors tested this hypothesis by examining the levels of soluble intercellular adhesion molecule and vascular cell adhesion molecule (sICAM-1 and sVCAM-1), and the presence of circulating endothelial cells (CECs), as evidence of vascular damage, in peripheral blood from DHF patients (n=13). A significant increase in plasma levels of sICAM-1 (n=12) and sVCAM-1 (n=13) was detected by enzyme-linked immunosorbent assay (ELISA) in DHF patients, compared with healthy individuals. Increased numbers of CECs, as detected by the expression of endothelial cell markers (ICAM-1, platelet cell adhesion molecule [PCAM]-1, and CD36) with flow cytometry, were observed in DHF patients (n=4), compared to healthy subjects. The high levels of sICAM-1 and sVCAM-1, together with the presence of CECs in DHF patients, provide further evidence of endothelium damage and activation in DHF patients.
authors.affiliation: Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela. jcardier@ivic.ve
authors.forename: José E
authors.initials: JE
authors.lastname: Cardier
authors.forename: Betty
authors.initials: B
authors.lastname: Rivas
authors.forename: Egidio
authors.initials: E
authors.lastname: Romano
authors.forename: Alan L
authors.initials: AL
authors.lastname: Rothman
authors.forename: Carlos
authors.initials: C
authors.lastname: Perez-Perez
authors.forename: Mariangel
authors.initials: M
authors.lastname: Ochoa
authors.forename: Ana M
authors.initials: AM
authors.lastname: Caceres
authors.forename: Marisabel
authors.initials: M
authors.lastname: Cardier
authors.forename: Napoleón
authors.initials: N
authors.lastname: Guevara
authors.forename: Roberto
authors.initials: R
authors.lastname: Giovannetti
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003715:Dengue / Q000150:complications* / Q000503:physiopathology; D042783:Endothelial Cells / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D008297:Male; D008875:Middle Aged; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood; D014652:Vascular Diseases / Q000209:etiology* / Q000473:pathology
chemical_list: D015815:Cell Adhesion Molecules; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1