title: A scaleable and defined system for generating neural stem cells from human embryonic stem cells.
abstract: The ability to differentiate human ESCs (hESCs) to defined lineages in a totally controlled manner is fundamental to developing cell-based therapies and studying human developmental mechanisms. We report a novel, scaleable, and widely applicable system for deriving and propagating neural stem cells from hESCs without the use of animal products, proprietary formulations, or genetic manipulation. This system provides a definitive platform for studying human neural development and has potential therapeutic implications.
authors.affiliation: Cambridge Centre for Brain Repair, Department of Clinical Neurosciences, Cambridge, CB2 2PY United Kingdom.
authors.forename: Alexis J
authors.initials: AJ
authors.lastname: Joannides
authors.forename: Christelle
authors.initials: C
authors.lastname: Fiore-Hériché
authors.forename: Alysia A
authors.initials: AA
authors.lastname: Battersby
authors.forename: Pandula
authors.initials: P
authors.lastname: Athauda-Arachchi
authors.forename: Isabelle A
authors.initials: IA
authors.lastname: Bouhon
authors.forename: Lydia
authors.initials: L
authors.lastname: Williams
authors.forename: Kristine
authors.initials: K
authors.lastname: Westmore
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Kemp
authors.forename: Alastair
authors.initials: A
authors.lastname: Compston
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Allen
authors.forename: Siddharthan
authors.initials: S
authors.lastname: Chandran
mesh_terms: D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D009474:Neurons / Q000166:cytology* / Q000502:physiology*