title: [Analysis of POU1F1 gene polymorphisms in Qinchuan cattle and Chinese Holstein cattle].
abstract: PCR-RFLP was applied to analyze the polymorphisms of POU1F1 gene in 218 Qinchuan cattle (QQ) and Chinese Holstein cattle (HC). Results demonstrated Hinf I polymorphisms in the 451 bp PCR product in the two populations. The frequencies of alleles A/B in QQ and HC populations were 0.232/0.768 and 0.132/0.868, respectively. The frequencies of three genotypes AA, AB and BB were 0.030/0.403/0.567 and 0.007/0.251/0.742, respectively. Qinchuan cattle population was at Hardy-Weinberg equilibrium at this locus, but Chinese Holstein cattle population was not. The gene heterozygosity/effective allele gene number/Shannon information entropy/polymorphism information content of Qinchuan cattle and Chinese Holstein cattle populations were listed for 0.356/1.553/0.541/0.292 and 0.229/1.297/0.390/0.203, respectively. All indices were higher in the Qinchuan cattle population.
authors.affiliation: Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China. ylj_23@sohu.com
authors.forename: Lin-Jun
authors.initials: LJ
authors.lastname: Yan
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Xin-Tang
authors.initials: XT
authors.lastname: Fang
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Run-Feng
authors.initials: RF
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Bao
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D002681:China; D005091:Exons / Q000235:genetics; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D007438:Introns / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D050817:Transcription Factor Pit-1 / Q000235:genetics*
chemical_list: D050817:Transcription Factor Pit-1