[CTL response to pre-erythrocytic stage vaccine candidate of plasmodium falciparum in HLA-A*0201 transgenic mice detected by ELISPOT assay].
The importance of cytotoxic T-lymphocyte (CTL) against malaria parasite in pre-erythrocytic stage has been presented in relevant researches. In order to investigate whether one CTL epitope (YLNKIQNSL) involved in a chimeric pre-erythrocytic stage vaccine candidate of Plasmodium falciparum which was expressed and purified in the laboratory can stimulate in vivo CTL response, HLA-A*0201 transgenic mice were immunized with this vaccine candidate. Enzyme-linked immunosorbent spot (ELISPOT) assay was performed on the splenocytes from the immunized transgenic mice. Positive result indicated that this CTL epitope can be in vivo processed and correctly presented.
Qian
Feng
F
Department of Etiological Biology, the Second Military Medical University, Shanghai, China.
Zhang
Qing-feng
QF
Shen
Lu-hui
LH
Pan
Wei-qing
WQ
D000818:Animals; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology*; D015234:HLA-A Antigens / Q000235:genetics*; D006801:Humans; D017780:Malaria Vaccines; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010963:Plasmodium falciparum / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic
D015234:HLA-A Antigens; D017780:Malaria Vaccines