Damage detection by the UvrABC pathway: crystal structure of UvrB bound to fluorescein-adducted DNA.
UvrB is the damage recognition element of the highly conserved UvrABC pathway that functions in the removal of bulky DNA adducts. Pivotal to this is the formation of a damage detection complex that relies on the ability of UvrB to locate and sequester diverse lesions. Whilst structures of UvrB bound to DNA have recently been reported, none address the issue of lesion recognition. Here, we describe the crystal structure of UvrB bound to a pentanucleotide containing a single fluorescein-adducted thymine that reveals a unique mechanism for damage detection entirely dependent on the exclusion of lesions larger than an undamaged nucleotide.
Waters
Timothy R
TR
The School of Crystallography and the Institute for Structural Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, United Kingdom.
Eryilmaz
Jitka
J
Geddes
Stella
S
Barrett
Tracey E
TE
D018360:Crystallography, X-Ray / Q000379:methods; D018736:DNA Adducts / Q000737:chemistry / Q000378:metabolism; D004249:DNA Damage; D004265:DNA Helicases / Q000737:chemistry / Q000378:metabolism*; D004706:Endodeoxyribonucleases / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
D018736:DNA Adducts; D029968:Escherichia coli Proteins; D009838:Oligodeoxyribonucleotides; C058093:UvrB protein, E coli; D004706:Endodeoxyribonucleases; C036385:endodeoxyribonuclease uvrABC; D004265:DNA Helicases