title: Incidence and prognostic value of tumour cells detected by RT-PCR in peripheral blood stem cell collections from patients with Ewing tumour.
abstract: To retrospectively evaluate the incidence of tumour cell contamination of peripheral blood stem cell (PBSC) collections and to correlate these data with the clinical outcome after high-dose chemotherapy (HDCT) with stem cell rescue in patients with a high-risk Ewing tumour. Peripheral blood stem cell collections obtained from 171 patients were analysed. Tumour contamination was assessed by reverse transcriptase-polymerase chain reaction (RT-PCR). The files of 88 patients who underwent HDCT followed by PBSC reinfusion were reviewed in detail, and their outcome compared to the PBSC RT-PCR results. Seven of 88 PBSC collections (8%) contained tumour cells as detected by RT-PCR. Peripheral blood stem cells were collected after a median of five cycles of chemotherapy. No clinical factor predictive of tumour cell contamination of PBSC harvest could be identified. Event-free survival (EFS) and overall survival (OS) of the whole study population were 45.3 % and 51.8 % at 3 years from the date of the graft, respectively. Forty-five patients relapsed with a median time of 15 months after graft, only four of whom had tumour cell contamination of the PBSC harvest. Tumour cell contamination of PBSC collection is rare and does not seem to be associated with a significantly poorer EFS or OS in this high-risk population.
authors.affiliation: Département d'Oncologie Pédiatrique, Institut Curie, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Vermeulen
authors.forename: S
authors.initials: S
authors.lastname: Ballet
authors.forename: O
authors.initials: O
authors.lastname: Oberlin
authors.forename: M
authors.initials: M
authors.lastname: Peter
authors.forename: G
authors.initials: G
authors.lastname: Pierron
authors.forename: E
authors.initials: E
authors.lastname: Longavenne
authors.forename: V
authors.initials: V
authors.lastname: Laurence
authors.forename: J
authors.initials: J
authors.lastname: Kanold
authors.forename: P
authors.initials: P
authors.lastname: Chastagner
authors.forename: O
authors.initials: O
authors.lastname: Lejars
authors.forename: J-Y
authors.initials: JY
authors.lastname: Blay
authors.forename: P
authors.initials: P
authors.lastname: Marec-Berard
authors.forename: J
authors.initials: J
authors.lastname: Michon
authors.forename: O
authors.initials: O
authors.lastname: Delattre
authors.forename: G
authors.initials: G
authors.lastname: Schleiermacher
mesh_terms: D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D019046:Bone Marrow Neoplasms / Q000235:genetics / Q000556:secondary; D002648:Child; D002675:Child, Preschool; D018572:Disease-Free Survival; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D007223:Infant; D007937:Leukapheresis; D008297:Male; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000378:metabolism; D051789:Proto-Oncogene Protein c-fli-1; D012333:RNA, Messenger / Q000032:analysis; D034802:RNA-Binding Protein EWS; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012512:Sarcoma, Ewing / Q000235:genetics / Q000473:pathology*; D012680:Sensitivity and Specificity; D015996:Survival Rate; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016896:Treatment Outcome
chemical_list: D018952:Antigens, CD34; C450460:EWS-ERG fusion protein, human; C106031:EWS-FLI fusion protein; D015514:Oncogene Proteins, Fusion; D051789:Proto-Oncogene Protein c-fli-1; D012333:RNA, Messenger; D034802:RNA-Binding Protein EWS; D014157:Transcription Factors; D016179:Granulocyte Colony-Stimulating Factor