High dead volume pumps: delay time reduction by short-term low-ratio split flow in LC/MS applications.
The aim of our work was to develop a low-cost, simple and reliable solution to reduce LC/MS analysis time by compensating for limitations inherent to high dead volume standard HPLC pumps. In our approach, we utilized a temporary (sub-1 min) low ratio flow split (approximately 1:10) at 5 mL/min pump flow before the column. During this short period, 90% of the entire pump flow is delivered to waste and used for fast pump/system equilibration. Although full-time flow splitting is widely used in capillary/nano applications (usually with high split ratios in the hundreds or thousands), to our knowledge, this is the first time that short-term low-ratio flow splitting has been used in conventional LC/MS applications.
Rogatsky
Eduard
E
General Clinical Research Center, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA. rogatsky@aecom.yu.edu
Stein
Daniel T
DT
D002499:Centrifugation, Density Gradient / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D013058:Mass Spectrometry / Q000379:methods*