title: Nucleocapsid amino acids 211 to 254, in particular, tetrad glutamines, are essential for the interaction between the nucleocapsid and membrane proteins of SARS-associated coronavirus.
abstract: GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N) interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted.
authors.affiliation: State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, PR China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Fang
authors.forename: Lin-Bai
authors.initials: LB
authors.lastname: Ye
authors.forename: Yijuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Baozong
authors.initials: B
authors.lastname: Li
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.forename: Lingbao
authors.initials: L
authors.lastname: Kong
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhenghui
authors.initials: Z
authors.lastname: Wu
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D000086462:Coronavirus Nucleocapsid Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D018698:Glutamic Acid / Q000235:genetics / Q000378:metabolism; D005973:Glutamine / Q000235:genetics / Q000378:metabolism*; D019251:Nucleocapsid / Q000378:metabolism*; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000378:metabolism*
chemical_list: D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; D010455:Peptides; D005973:Glutamine; C097188:polyglutamine; D018698:Glutamic Acid