Complex coacervation of silk fibroin and hyaluronic acid.
This study aimed to investigate the pH-induced complexation of silk fibroin (SF) and hyaluronic acid (HA). SF-HA complex coacervation was investigated by monitoring turbidity of the SF-HA system under slow acidification. Gravimetric analysis was performed to determine the yield of complex coacervation and viscosity of the system was measured to study the formation of the complexes at different pH values. The influences of total biopolymer concentration and biopolymer weight ratio on complex coacervation were examined during the analyses. Formation of the complexes was evidenced by the minimum viscosity and the maximum turbidity observed in the system. SF-HA complexes were formed within the pH-window of 2.5-3.5 regardless of the total biopolymer concentration or biopolymer ratio. Complex coacervation of SF-HA showed a reversible behavior and coacervation could be handled even in excess amounts of the biopolymers, which pointed out a non-stoichiometric complexation.
Malay
Ozge
O
Bioreaction Engineering Laboratory, Department of Chemical Engineering, Izmir Institute of Technology, Gülbahçe Köyü, 35430 Urla, Izmir, Turkey. ozgemalay@iyte.edu.tr
Bayraktar
Oguz
O
Batigün
Ayşegül
A
D000818:Animals; D001704:Biopolymers / Q000737:chemistry; D004586:Electrophoresis; D005349:Fibroins / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009391:Nephelometry and Turbidimetry; D047011:Silk / Q000737:chemistry*; D012995:Solubility; D017186:Titrimetry; D014783:Viscosity
D001704:Biopolymers; D047011:Silk; D006820:Hyaluronic Acid; D005349:Fibroins