[Comparative analysis of methods for diagnostics of chlamydial infection in women of reproductive age].
The aim of this study was to compare the effectiveness of three methods of diagnostics of chlamydial infection: polymerase chain reaction (PCR), direct immunofluorescence (DFA) and enzyme immunoassay (EIA). We calculated sensitivity, specificity, positive and negative predictive values for each method of diagnostics. Blood, cervical specimens for detection of Chlamydia trachomatis were obtained from 103 women of reproductive age. The results of investigation show that: 1. PCR, which has high sensitivity (92.1+/-3.4%) and specificity (95.0+/-3.4%), is an effective method of diagnostics of chlamydial infection. 2. While the positive results of PCR in cervical specimens are strong evidence of presence of chlamydial infections, the negative results of PCR in cervical specimens do not prove the absence of chlamydial infection in internal genital organs of women with infertility. For the diagnostics of chlamydial infection in women of reproductive age simultaneous use of two methods of diagnostics is recommended.
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D000293:Adolescent; D000328:Adult; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005189:False Positive Reactions; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006654:Histology, Comparative; D006801:Humans; D010535:Laparoscopy; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity