Purification of a trypsin inhibitor from sweet potato in an aqueous two phase system.
Trypsin inhibitor from sweet potato was extracted and purified in a single step using an aqueous two-phase system of polyethylene glycol 6000 (11% w/v), phosphate (16.5% w/v), KCl (9% w/v) and at pH 6. Purity of the trypsin inhibitor was enhanced 3.7-fold, and the recovery was 95%. The purified trypsin inhibitor showed one visible band, and the molecular size was 23 kDa by SDS-PAGE.
Jaw
Kuen-Shan
KS
Department of Bioengineering, Tatung University, Taipei, Taiwan.
Chou
Lin Hung
LH
Chang
Shiao-Ming
SM
Duan
Kow-Jen
KJ
D027723:Ipomoea batatas / Q000378:metabolism*; D010936:Plant Extracts / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D014361:Trypsin Inhibitors / Q000737:chemistry* / Q000302:isolation & purification*
D010936:Plant Extracts; D014361:Trypsin Inhibitors