Individually resolved DNA molecules stretched and embedded in electrospun polymer nanofibers.
We have used the flow characteristics of an electrospinning jet to elongate and fix DNA molecules. We embedded and observed fluorescently labeled lambda bacteriophage DNA molecules in polyethylene oxide nanofibers. The embedded DNA molecules were imaged using fluorescence microscopy and found to be stretched to lengths approaching the full dyed contour length.
Bellan
Leon M
LM
School of Applied and Engineering Physics and Department of Physics, Cornell University, Ithaca, New York 14853, USA. Lmb79@cornell.edu
Cross
Joshua D
JD
Strychalski
Elizabeth A
EA
Moran-Mirabal
Jose
J
Craighead
H G
HG
D010582:Bacteriophage lambda / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods; D043942:Nanotubes / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors
D011092:Polyethylene Glycols; D004247:DNA