Fortuitous structure determination of 'as-isolated' Escherichia coli bacterioferritin in a novel crystal form.
Escherichia coli bacterioferritin was serendipitously crystallized in a novel cubic crystal form and its structure could be determined to 2.5 A resolution despite a high degree of merohedral twinning. This is the first report of crystallographic data on 'as-isolated' E. coli bacterioferritin. The ferroxidase active site contains positive difference density consistent with two metal ions that had co-purified with the protein. X-ray fluorescence studies suggest that the metal composition is different from that of previous structures and is a mix of zinc and native iron ions. The ferroxidase-centre configuration displays a similar flexibility as previously noted for other bacterioferritins.
van Eerde
André
A
Laboratory of Biophysical Chemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Wolterink-van Loo
Suzanne
S
van der Oost
John
J
Dijkstra
Bauke W
BW
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002570:Ceruloplasmin / Q000737:chemistry / Q000302:isolation & purification; D003300:Copper / Q000032:analysis; D018360:Crystallography, X-Ray / Q000379:methods; D003573:Cytochrome b Group / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000201:enzymology; D005293:Ferritins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D007501:Iron / Q000032:analysis; D008345:Manganese / Q000032:analysis; D008958:Models, Molecular; D011487:Protein Conformation; D015032:Zinc / Q000032:analysis
D001426:Bacterial Proteins; D003573:Cytochrome b Group; D008345:Manganese; D003300:Copper; D005293:Ferritins; C018032:bacterioferritin; D007501:Iron; D002570:Ceruloplasmin; D015032:Zinc