Depth-resolved imaging of functional activation in the rat cerebral cortex using optical coherence tomography.
Co-registered optical coherence tomography (OCT) and video microscopy of the rat somatosensory cortex were acquired simultaneously through a thinned skull during forepaw electrical stimulation. Fractional signal change measured by OCT revealed a functional signal time course corresponding to the hemodynamic signal measurement made with video microscopy. OCT can provide high-resolution, cross-sectional images of functional neurovascular activation and may offer a new tool for basic neuroscience research in the important rat cerebral cortex model.
Aguirre
A D
AD
Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Chen
Y
Y
Fujimoto
J G
JG
Ruvinskaya
L
L
Devor
A
A
Boas
D A
DA
D000818:Animals; D001931:Brain Mapping / Q000379:methods*; D002540:Cerebral Cortex / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation* / Q000379:methods; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods; D051381:Rats; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods