title: Peripheral mononuclear cell response in Japanese black calves after oral administration of IFN-alpha.
abstract: To investigate the effects of oral administration of an interferon (IFN)-alpha drug on the immune reaction of healthy Japanese Black (JB) calves, peripheral leukocyte populations and their ability to produce cytokine mRNA were analyzed after oral administration of IFN-alpha. Fourteen calves fed in one herd were divided into two groups; seven calves were orally administered 0.1 g/day of IFN-alpha from the day of birth to day 5 on each day (group 1, N=7), and the other seven calves were used as the control (group 2, N=7). Blood samples were collected from the jugular veins of all calves before administration and in weeks 1, 2, 4, 8, and 16 after birth. The number of MHC class II+CD14+ monocytes in the leukocytes population of group 1 increased gradually after birth, and significantly higher numbers were detected in week 4 compared with group 2. MHC class II-CD14+ monocytes in group 1 peaked in week 1, and a significant increase was detected compared with group 2. The level of IL-12 in the cytokine mRNA of group 1 increased gradually between weeks 1 and 2, and a significantly higher level of IL-12 was found compared with group 2. These results suggest that oral administration of IFN-alpha induces activation of the monocyte functions in JB calves.
authors.affiliation: School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Mayumi
authors.initials: M
authors.lastname: Tokita
authors.forename: Katsushige
authors.initials: K
authors.lastname: Takahashi
authors.forename: Machiko
authors.initials: M
authors.lastname: Masui
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kohiruimaki
authors.forename: Tomohito
authors.initials: T
authors.lastname: Hayashi
authors.forename: Takaaki
authors.initials: T
authors.lastname: Ando
authors.forename: Daisaku
authors.initials: D
authors.lastname: Watanabe
authors.forename: Seiichi
authors.initials: S
authors.lastname: Kawamura
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002417:Cattle / Q000276:immunology*; D005434:Flow Cytometry / Q000662:veterinary; D007111:Immunity, Cellular / Q000187:drug effects; D016130:Immunophenotyping / Q000662:veterinary; D016898:Interferon-alpha / Q000235:genetics / Q000494:pharmacology*; D007378:Interleukins / Q000235:genetics / Q000276:immunology; D007958:Leukocyte Count / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D016898:Interferon-alpha; D007378:Interleukins; D012313:RNA