Angiotensin II induces NF-kappa B activation in HUVEC via the p38MAPK pathway.
Angiotensin II (Ang II) is the main active peptide of the renin-angiotensin system (RAS), producing a number of inflammatory mediators that lead to endothelial dysfunction and the progression of atherosclerosis. Ang II-induced NF-kappaB nuclear translocation plays a pivotal role in this response. This study examines the NF-kappaB activation mechanism elicited by Ang II in human umbilical vein endothelial cells (HUVEC). Electrophoretic mobility shift assays and Western blotting revealed that Ang II, signaling via AT(1), produces a time-dependent increase in NF-kappaB DNA binding and IkappaBalpha degradation. These results also demonstrate that Ang II leads to MAPK phosphorylation and p38MAPK pathway-induced NF-kappaB activation. Furthermore, AT(1) is required for p38MAPK phosphorylation induced by Ang II. This study provides evidence that Ang II elicits NF-kappaB activation via the p38MAPK pathway in HUVEC.
Guo
Rui-Wei
RW
Department of Cardiology, Kunming General Hospital of Chengdu Military Area, Yunnan 650032, China. grw771210@163.com
Yang
Li-Xia
LX
Li
Mao-Quan
MQ
Liu
Bei
B
Wang
Xian-Mei
XM
D000804:Angiotensin II / Q000502:physiology*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism*; D011485:Protein Binding; D044140:Receptor, Angiotensin, Type 1 / Q000502:physiology; D048051:p38 Mitogen-Activated Protein Kinases / Q000502:physiology*
D016328:NF-kappa B; D044140:Receptor, Angiotensin, Type 1; D000804:Angiotensin II; D004247:DNA; D048051:p38 Mitogen-Activated Protein Kinases