Calpain activation in apoptosis of motoneurons in cell culture models of experimental parkinsonism.
Parkinson's disease (PD) is a movement disorder characterized by progressive degeneration of primarily the dopaminergic neurons in the substantia nigra (SN). The present study briefly describes our findings to support the hypothesis that there is a possibility of degeneration of spinal cord (SC) motoneurons in course of parkinsonism. In cell culture models of experimental parkinsonism, we examined the degeneration of ventral SC motoneuron cell line (VSC4.1) following exposure to two different toxins, such as 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and rotenone. Our studies suggested calpain activation in the apoptosis of VSC4.1 motoneurons due to exposure to these parkinsonian toxins. Furthermore, our study showed the toxic effects of the dopaminergic toxin methamphetamine (METH) on VSC4.1 cells. The results strongly implicated a possible role for calpain in the mechanism of motoneuron apoptosis during parkinsonian degeneration, at large. Hence, we examined the neuroprotective efficacy of calpeptin, a specific inhibitor of calpain, in cell culture model of experimental parkinsonism.
Samantaray
Supriti
S
Department of Neurosciences, Division of Neurology, Medical University of South Carolina, 96 Jonathan Lucas Street, Charleston, SC 29425, USA.
Ray
Swapan K
SK
Ali
Syed F
SF
Banik
Naren L
NL
D000818:Animals; D017209:Apoptosis; D002154:Calpain / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018929:Cell Culture Techniques; D053938:DNA Fragmentation; D004151:Dipeptides / Q000494:pharmacology*; D004195:Disease Models, Animal; D006801:Humans; D020287:In Situ Nick-End Labeling; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology*; D020734:Parkinsonian Disorders / Q000628:therapy*; D051381:Rats
D004151:Dipeptides; C071482:calpeptin; D002154:Calpain