title: A mycobacterial gene involved in synthesis of an outer cell envelope lipid is a key factor in prevention of phagosome maturation.
abstract: Virulent mycobacteria cause arrest of phagosome maturation as a part of their survival strategy in hosts. This process is mediated through multiple virulence factors, whose molecular nature remains elusive. Using Mycobacterium marinum as a model, we performed a genome-wide screen to identify mutants whose ability to inhibit phagosome maturation was impaired, and we succeeded in isolating a comprehensive set of mutants that were not able to occupy an early endosome-like phagosomal compartment in mammalian macrophages. Categorizing and ordering the multiple mutations according to their gene families demonstrated that the genes modulating the cell envelope are the principal factors in arresting phagosome maturation. In particular, we identified a novel gene, pmiA, which is capable of influencing the constitution of the cell envelope lipids, thereby leading to the phagosome maturation block. The pmiA mutant was not able to resist phagosome maturation and was severely attenuated in mice. Complementing the mutant with the wild-type gene restored the attenuated virulence to wild-type levels in mice.
authors.affiliation: Institute for Medical Microbiology, University of Cologne, 50935 Cologne, Germany.
authors.forename: Nirmal
authors.initials: N
authors.lastname: Robinson
authors.forename: Martina
authors.initials: M
authors.lastname: Wolke
authors.forename: Karen
authors.initials: K
authors.lastname: Ernestus
authors.forename: Georg
authors.initials: G
authors.lastname: Plum
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002855:Chromatography, Thin Layer; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D017353:Gene Deletion; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008264:Macrophages / Q000382:microbiology; D008563:Membrane Lipids / Q000032:analysis / Q000302:isolation & purification; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology; D019910:Mycobacterium marinum / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D010588:Phagosomes / Q000737:chemistry / Q000382:microbiology* / Q000502:physiology; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008563:Membrane Lipids; D037521:Virulence Factors