Developmentally regulated expression of MSX1, MSX2 and Fgfs in the developing mouse cranial base.
OBJECTIVE
To examine the expression pattern of the Fgf and Msx genes in cranial base development.


METHODS
To detect the expression of these genes, antisense riboprobes were synthesized by in vitro transcription. Radioactive in situ hybridization was performed on parasagittal sections of embryonic mouse heads.


RESULTS
Msx2 was observed in the underlying perichondrium at restricted stages. Msx1 was not observed in cranial base development. Fgf1 was localized in osteogenic cells from the time of ossification; Fgf10 was highly expressed in the occipital-vertebral joint during E13 to E14; Fgf2, Fgf7, and Fgf18 were localized in the perichondria; Fgf12 was transitorily expressed at early chondrocranium; Fgf9 was seen in the hypertrophic chondrocytes.


CONCLUSIONS
The Fgf and Msx gene expression in the cranial base was different from that of other skeletons.
Nie
Xuguang
X
Department of Biomedicine, University of Bergen, Bergen, Norway. Xuguang.Nie@biomed.uib.no
D000818:Animals; D001270:Cervical Atlas / Q000254:growth & development; D019902:Chondrocytes / Q000378:metabolism; D020219:Chondrogenesis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D005346:Fibroblast Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox / Q000502:physiology; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D017403:In Situ Hybridization; D051957:MSX1 Transcription Factor / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics*; D010902:Pituitary Gland / Q000254:growth & development; D019291:Skull Base / Q000254:growth & development*
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D051957:MSX1 Transcription Factor; C084119:MSX2 protein; D005346:Fibroblast Growth Factors