title: Advantages of LC-MS-MS compared to LC-MS for the determination of nitrofuran residues in honey.
abstract: In the framework of developing analyses for exogenous contaminants in food matrices such as honey, we have compared data obtained by high-performance liquid chromatography coupled with mass spectrometry (LC-MS) to those provided by high-performance liquid chromatography and tandem mass spectrometry (LC-MS-MS). Initial results obtained with LC-MS showed that the technique lacked selectivity, which is why the method was validated by LC-MS-MS. This method involves a solid-phase extraction (SPE) of nitrofuran metabolites and nitrofuran parent drugs, a derivatization by 2-nitrobenzaldehyde for 17 h, and finally a clean-up by SPE. The data obtained show that the limits of detection varied between 0.2 and 0.6 microg kg(-1) for the metabolites and between 1 and 2 microg kg(-1) for nitrofuran parent drugs. The method was applied to different flower honeys. The results showed that nitrofurans (used as antibiotics) are consistently present in this matrix, the predominant compound being furazolidone. Figure Working bees.
authors.affiliation: Service Central d'Analyse, CNRS-USR 059, BP 22, 69390 Vernaison, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Tribalat
authors.forename: Olivier
authors.initials: O
authors.lastname: Paisse
authors.forename: Guy
authors.initials: G
authors.lastname: Dessalces
authors.forename: Marie-Florence
authors.initials: MF
authors.lastname: Grenier-Loustalot
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006722:Honey / Q000032:analysis* / Q000145:classification; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D009581:Nitrofurans / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D015203:Reproducibility of Results; D012996:Solutions
chemical_list: D007477:Ions; D009581:Nitrofurans; D012996:Solutions