Beneficial effects of prolactin and laminin on human pancreatic islet-cell cultures.
The problem of pancreas donor shortage could be addressed through in vitro islet-cell proliferation prior to transplantation into diabetic patients. Therefore, we set out to evaluate the effects of prolactin (rhPRL) and laminin on primary cultures of human pancreatic islets. Our results showed that rhPRL induced an increase in islet-cell number and in cumulative insulin secretion (p<0.01). However, glucose-induced insulin secretion was enhanced only in the presence of both laminin and rhPRL. In addition, we describe, for the first time in human islets, the PRL-induced activation of JAK2, and signal transducer and activator of transcription (STAT) 1, 3 and 5. Our results demonstrate a significant beneficial effect of rhPRL and laminin on human islets and support widely held notion that the closer physiological stimuli and environment of beta cells are mimicked, the better are the results in cell proliferation and secretory function, both essential for successful islet transplantation.
Labriola
Leticia
L
Chemistry Institute, University of São Paulo, and Albert Einstein Hospital, Brazil.
Montor
Wagner R
WR
Krogh
Karin
K
Lojudice
Fernando H
FH
Genzini
Tércio
T
Goldberg
Anna C
AC
Eliaschewitz
Freddy G
FG
Sogayar
Mari C
MC
D000328:Adult; D000368:Aged; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005455:Fluorescent Antibody Technique; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D047468:Immunoprecipitation; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D053614:Janus Kinase 2 / Q000378:metabolism; D007797:Laminin / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D011388:Prolactin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050791:STAT Transcription Factors / Q000378:metabolism
D007328:Insulin; D007797:Laminin; D012333:RNA, Messenger; D050791:STAT Transcription Factors; D011388:Prolactin; D053614:Janus Kinase 2; D005947:Glucose