title: Plasmodium yoelii: the effect of second blood meal and anti-sporozoite antibodies on development and gene expression in the mosquito vector, Anopheles stephensi.
abstract: The sporogonic development of the malaria parasite takes place in the mosquito and a wide range of factors modulates it. Among those, the contents of the blood meal can influence the parasite development directly or indirectly through the mosquito response to the infection. We have studied the effect of a second blood meal in previously infected mosquitoes and the effect of anti-sporozoite immune serum on parasite development and mosquito response to the infection. The prevalence and intensity of infection and gene expression of both Plasmodium yoelii and Anopheles stephensi was analyzed. We verified that a second blood meal and its immune status interfere with parasite development and with Plasmodium and mosquito gene expression.
authors.affiliation: Centro de Malária e Outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira 96, 1349-008 Lisboa, Portugal. filipe@ihmt.unl.pt
authors.forename: L F
authors.initials: LF
authors.lastname: Lopes
authors.forename: P
authors.initials: P
authors.lastname: Abrantes
authors.forename: A P
authors.initials: AP
authors.lastname: Silva
authors.forename: V E
authors.initials: VE
authors.lastname: DoRosario
authors.forename: H
authors.initials: H
authors.lastname: Silveira
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D001769:Blood; D016054:DNA, Protozoan / Q000737:chemistry; D005247:Feeding Behavior / Q000502:physiology; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000276:immunology; D007303:Insect Vectors / Q000469:parasitology*; D051379:Mice; D010967:Plasmodium yoelii / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D016053:RNA, Protozoan / Q000235:genetics; D051381:Rats; D017421:Sequence Analysis; D034101:Sporozoites / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D016053:RNA, Protozoan