title: [Expression of total RNA and amplification of myocardial troponin gene during monitoring viral cardiomyocyte injury.].
abstract: BACKGROUND
  To investigate the value of circulating cTnI-mRNA detection for monitoring myocardial injury development and prognosis.
  
  
  METHODS
  Viral myocardial injury models in BALB/c mice were created by intraperitoneal inoculation with Coxsackievirus B3 (CVB3,1x108 TCID50) for inducing myocardial injury. The total RNAs were extracted and cTnI-mRNA in mice cardiac tissues and circulating blood were amplified by RT-PCR during mice myocardial injury.
  
  
  RESULTS
  In virus infected mice, the mRNA abundance for cTnI was up-regulated in heart and circulating blood and associated with salient myocardial histopathologic features, including myocardial swelling, inflammatory cell infiltration, pyknosis, karyorrhexis, karyolysis, denaturalization, necrosis, and calcification. The cTnI-mRNA form infected mice heart and circulating cardiac myocytes were analyzed by RT-PCR, the amplified gene fragments were found in all heart tissues. The incidence of cTnI-mRNA was 0, 0, 0, 16.7%, 40.0%, 71.4%, 83.3% and 87.5% in the controls, the 3rd, 6th, 9th, 12th, 15th, 18th,and 21st day in circulating bloods from the infected mice, respectively.
  
  
  CONCLUSIONS
  The present data suggest that cTnI-mRNA expression is up-regulated and released into blood on viral myocardial injury, and detection of circulating cTnI-mRNA is a sensitive genetic marker for monitoring myocardial injury development and prognosis.
authors.affiliation: Affiliated Hospital, Nantong Medical College, Nantong 226001, China. Corresponding author: YAO Deng-fu, E-mail: yaodf@ahnmc.com, Tel: 13806298368.
authors.forename: W
authors.initials: W
authors.lastname: Wu
authors.forename: D F
authors.initials: DF
authors.lastname: Yao
authors.forename: J H
authors.initials: JH
authors.lastname: Zhu
authors.forename: Z D
authors.initials: ZD
authors.lastname: Gao
authors.forename: X H
authors.initials: XH
authors.lastname: Wu
authors.forename: L W
authors.initials: LW
authors.lastname: Qiu
mesh_terms: D000818:Animals; D029822:Enterovirus B, Human; D008807:Mice, Inbred BALB C; D009205:Myocarditis; D009206:Myocardium; D032383:Myocytes, Cardiac; D012313:RNA; D019210:Troponin I
chemical_list: D019210:Troponin I; D012313:RNA