Simple colorimetric inhibition assay of heme crystallization for high-throughput screening of antimalarial compounds.
Current assays for screening new antimalarials need initiators of beta-hematin formation that require laborious preparation, special devices, and substrates. In this study, based on reduction of heme absorption in beta-hematin formation, we developed a simple colorimetric assay using Tween 20 as an initiator and a microplate reader for high-throughput screening of inhibitors of beta-hematin formation.
Huy
Nguyen Tien
NT
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Uyen
Dinh Thanh
DT
Maeda
Atsushi
A
Trang
Dai Thi Xuan
DT
Oida
Tatsuo
T
Harada
Shigeharu
S
Kamei
Kaeko
K
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D003124:Colorimetry / Q000379:methods; D003460:Crystallization; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006418:Heme / Q000737:chemistry / Q000378:metabolism*; D006420:Hemeproteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000378:metabolism; D011136:Polysorbates / Q000737:chemistry; D015203:Reproducibility of Results
D000962:Antimalarials; D006420:Hemeproteins; D011136:Polysorbates; D006418:Heme