Effects of Glomus mosseae on the toxicity of heavy metals to Vicia faba.
A glasshouse pot experiment was conducted to investigate effects of the arbuscular mycorrhizal fungus Glomus mosseae on the growth of Vicia faba and toxicity induced by heavy metals (HMs) (Cu, Zn, Pb and Cd) in a field soil contaminated by a mixture of these metals. There was also uninoculation treatment (NM) simultaneously. Mycorrhizal (GM) plants hav e significantlyincreased growth and tolerance to toxicity induced by heavy metals compared with NM plants. P uptake was significantly increased in GM plants. Mycorrhizal symbiosis reduced the transportation of HMs from root to shoot by immobilizing HMs in the mycorrhizal, shown by increasing the ratios of HMs from root to shoot. Oxidative stress, which can induce DNA damage, is an important mechanism of heavy metal toxicity. GM treatment decreased oxidative stress by intricating antioxidative systems such as peroxidases and non-enzymic systems including soluble protein. The DNA damage induced by heavy metals was detected using comet assay, which showed DNA damage in the plants was decreased by the GM treatment.
Zhang
Xu-hong
XH
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing.
Lin
Ai-jun
AJ
Chen
Bao-dong
BD
Wang
You-shan
YS
Smith
Sally E
SE
Smith
F Andrew
FA
D020552:Comet Assay; D004249:DNA Damage; D019216:Metals, Heavy / Q000633:toxicity*; D038821:Mycorrhizae / Q000502:physiology*; D018384:Oxidative Stress; D010544:Peroxidases / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism / Q000633:toxicity; D013559:Symbiosis; D031307:Vicia faba / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology
D019216:Metals, Heavy; D012989:Soil Pollutants; D010544:Peroxidases