title: Drinking of Salvia officinalis tea increases CCl(4)-induced hepatotoxicity in mice.
abstract: In a previous study, the drinking of a Salvia officinalis tea (prepared as an infusion) for 14 days improved liver antioxidant status in mice and rats where, among other factors, an enhancement of glutathione-S-transferase (GST) activity was observed. Taking in consideration these effects, in the present study the potential protective effects of sage tea drinking against a situation of hepatotoxicity due to free radical formation, such as that caused by carbon tetrachloride (CCl(4)), were evaluated in mice of both genders. Contrary to what was expected, sage tea drinking significantly increased the CCl(4)-induced liver injury, as seen by increased plasma transaminase levels and histology liver damage. In accordance with the previous study, sage tea drinking enhanced significantly GST activity. Additionally, glutathione peroxidase was also significantly increased by sage tea drinking. Since CCl(4) toxicity results from its bioactivation mainly by cytochrome P450 (CYP) 2E1, the expression level of this protein was measured by Western Blot. An increase in CYP 2E1 protein was observed which may explain, at least in part, the potentiation of CCl(4)-induced hepatotoxicity conferred by sage tea drinking. The CCl(4)-induced hepatotoxicity was higher in females than males. In conclusion, our results indicate that, although sage tea did not have toxic effects of its own, herb-drug interactions are possible and may affect the efficacy and safety of concurrent medical therapy with drugs that are metabolized by phase I enzymes.
authors.affiliation: Department/Centre of Biology, School of Sciences, University of Minho, 4710-057 Braga, Portugal.
authors.forename: Cristovao F
authors.initials: CF
authors.lastname: Lima
authors.forename: Manuel
authors.initials: M
authors.lastname: Fernandes-Ferreira
authors.forename: Cristina
authors.initials: C
authors.lastname: Pereira-Wilson
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001628:Beverages; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000517:prevention & control; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D005260:Female; D041743:Herb-Drug Interactions; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D027543:Salvia officinalis
chemical_list: D000975:Antioxidants; D002251:Carbon Tetrachloride; D019392:Cytochrome P-450 CYP2E1