Prospective, paired crossover comparison of multiple, single-needle plateletpheresis procedures with the Amicus and Trima Accel cell separators.
BACKGROUND
The Baxter Amicus Version 2.51 (A) and the Gambro BCT Trima Accel Version 5.0 (T) cell separators may produce multiple platelet (PLT) concentrates within a single donation.


METHODS
The single-needle multiple plateletpheresis procedures of the two devices were compared in a prospective, randomized, paired crossover study in 60 donors. The 120 donations were compared for donor comfort, collection efficiency, residual white blood cell (WBC) count, and (in selected patients) corrected count increment (CCI).


RESULTS
The mean PLT yield and the resultant mean number of units per donation were significantly lower for A (6.06 x 10(11) vs. 7.48 x 10(11) and 2.57 vs. 3.19, respectively, both p < 0.001), in spite of a longer apheresis duration (89 min vs. 79 min; p < 0.001). This resulted in a higher collection rate of T (5.68 x 10(11) PLTs/hr vs. 4.10 x 10(11) PLTs/hr, p < 0.001). Residual WBC count of every unit was fewer than 5 x 10(6), but significantly fewer A-PLT donations contained more than 10(5) WBCs per unit (1 vs. 9, p = 0.008). Although the ACD-A consumption was slightly higher for A (489 mL vs. 469 mL, p = 0.04), a trend to a higher frequency of side effects was found for T (42.4% vs. 23.7%, p = 0.06). The 1-hour CCIs of 33 transfused A-PLT units were comparable with those of 43 T-PLT units (11.8 vs. 13.9, p = 0.480).


CONCLUSIONS
Both cell separators showed safe collections of up to 4 PLT units per donation with adequate CCI. T produced a higher PLT yield despite shorter apheresis duration, but with slightly higher residual WBC counts and a trend to a higher side-effect frequency.
Fontana
Stefano
S
Department of Hematology and Central Hematology Laboratory, Division of Transfusion Medicine, Inselspital, University Hospital, Bern, Switzerland. stefano.fontana@insel.ch
Mordasini
Livio
L
Keller
Peter
P
Taleghani
Behrouz Mansouri
BM
D001782:Blood Donors; D001792:Blood Platelets / Q000166:cytology*; D018592:Cross-Over Studies; D005260:Female; D006801:Humans; D007958:Leukocyte Count / Q000379:methods; D008297:Male; D009339:Needles; D017713:Platelet Transfusion / Q000009:adverse effects / Q000379:methods; D010983:Plateletpheresis / Q000295:instrumentation / Q000379:methods; D011446:Prospective Studies