Mycobacterium tuberculosis malate synthase- and MPT51-based serodiagnostic assay as an adjunct to rapid identification of pulmonary tuberculosis.
The 81-kDa malate synthase (MS; Rv 1837c) and the 27-kDa MPT51 (Rv 3803c) of Mycobacterium tuberculosis are immunodominant antigens recognized by serum antibodies from approximately 80% of human immunodeficiency virus-negative smear-positive tuberculosis patients from India. We now provide evidence that the use of the MS/MPT51-based serodiagnostic assay can serve as an adjunct to sputum microscopy in the rapid diagnosis of pulmonary tuberculosis.
Achkar
Jacqueline M
JM
New York University School of Medicine, New York, New York 10016, USA. jachkar@aecom.yu.edu
Dong
Yuxin
Y
Holzman
Robert S
RS
Belisle
John
J
Kourbeti
Irene S
IS
Sherpa
Tsering
T
Condos
Rany
R
Rom
William N
WN
Laal
Suman
S
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D006801:Humans; D008292:Malate Synthase / Q000276:immunology*; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000276:immunology / Q000302:isolation & purification*; D010865:Pilot Projects; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000201:enzymology / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C082911:MPT51 protein, Mycobacterium; D008292:Malate Synthase