Effect of L-serine on the biosynthesis of aromatic amino acids in Escherichia coli.
It is well known that some amino acids inhibit bacterial growth. L-Serine is known to inhibit the growth of Escherichia coli by inhibition of homoserine dehydrogenase (EC 1.1.1.3). It has been reported that this L-serine inhibition may be prevented by the addition of L-isoleucine or L-threonine to the medium. In our study, however, recovery of the growth inhibition of Escherichia coli by L-serine occurred in the presence of several amino acids, especially L-phenylalanine. In an attempt to further elucidate this inhibition mechanism, different intermediates of aromatic amino acid biosynthesis were added to the growth medium. Recovery from the inhibition did not occur in the presence of prephenate but did occur when phenylpyruvate was added to the medium. The specific activity of prephenate dehydratase decreased in cells grown in the presence of L-serine. However. L-serine did not inhibit in vitro prephenate dehydratase activity, and the expression of pheA, which encodes the prephenate dehydratase, was not depressed by L-serine. We suggest that L-serine acts via another inhibition mechanism. Although this inhibition mechanism has not been fully elucidated, our results suggest that the addition of L-serine to the growth medium inhibits prephenate dehydratase synthesis and thus affects L-phenylalanine biosynthesis.
Tazuya-Murayama
Keiko
K
Daiichi College of Pharmaceutical Sciences, 2-11, Tamagawa-cho, Minami-ku, Fukuoka 815-8 511, Japan.
Aramaki
Hironori
H
Mishima
Motohiro
M
Saito
Keiko
K
Ishida
Shiho
S
Yamada
Kazuko
K
D000596:Amino Acids / Q000494:pharmacology; D024322:Amino Acids, Aromatic / Q000096:biosynthesis*; D002364:Caseins / Q000494:pharmacology; D003470:Culture Media; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D010649:Phenylalanine / Q000096:biosynthesis / Q000494:pharmacology; D011302:Prephenate Dehydratase / Q000037:antagonists & inhibitors; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012694:Serine / Q000494:pharmacology*; D014364:Tryptophan / Q000096:biosynthesis / Q000494:pharmacology; D014443:Tyrosine / Q000096:biosynthesis / Q000494:pharmacology
D000596:Amino Acids; D024322:Amino Acids, Aromatic; D002364:Caseins; D003470:Culture Media; D004791:Enzyme Inhibitors; D014443:Tyrosine; D012694:Serine; D010649:Phenylalanine; D014364:Tryptophan; D011302:Prephenate Dehydratase