Fertilization and pregnancy outcome after intracytoplasmic injection with fresh or cryopreserved ejaculated spermatozoa.
OBJECTIVE
To assess fertilization, implantation, and pregnancy rates in patients undergoing ICSI using fresh and cryopreserved sperm from ejaculated semen samples.


METHODS
Retrospective study.


METHODS
Academic and private medical centers.


METHODS
One hundred fifty-eight patients.


METHODS
Intracytoplasmic sperm injection.


METHODS
Fertilization, implantation, and pregnancy rates were evaluated; 61 cycles of ICSI were performed with cryopreserved and 79 cycles of ICSI were performed with fresh spermatozoa. Also, we divided the outcomes according to the semen characteristics, normozoospermia, oligozoospermia, asthenozoospermia, and oligoasthenozoospermia.


RESULTS
Overall, normal-fertilization rates were higher using fresh sperm (73.8%) compared with cryopreserved sperm (68.7%). Cycles performed in patients with normozoospermia or oligozoospermia had similar fertilization, implantation, and pregnancy rates using fresh or cryopreserved sperm. When asthenozoospermic and oligoasthenozoospermic semen samples were used, the normal-fertilization rate was higher with fresh sperm compared with cryopreserved sperm. However, implantation and pregnancy rates were similar in fresh and cryopreserved sperm samples from patients with asthenozoospermia or oligoasthenozoospermia.


CONCLUSIONS
Semen with abnormalities in the motility may be more susceptible to sperm cryopreservation damage, resulting in lower fertilization rates. However, once the oocyte is fertilized, implantation and pregnancy rates are similar to those in patients with oligozoospermia and normozoospermia.
Borges
Edson
E
Fertility-Assisted Fertilization Center, São Paulo, Brazil.
Rossi
Lia Mara
LM
Locambo de Freitas
Christiany Victor
CV
Guilherme
Patrícia
P
Bonetti
Tatiana Carvalho S
TC
Iaconelli
Assumpto
A
Pasqualotto
Fabio Firmbach
FF
D000328:Adult; D001938:Brazil / Q000453:epidemiology; D015925:Cryopreservation / Q000706:statistics & numerical data*; D004542:Ejaculation; D005260:Female; D005306:Fertilization; D006801:Humans; D007247:Infertility, Female / Q000453:epidemiology* / Q000628:therapy*; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000453:epidemiology*; D018873:Pregnancy Rate; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods / Q000706:statistics & numerical data*; D016896:Treatment Outcome