Immunization with outer membrane proteins of Pasteurella multocida (6:B) provides protection in mice.
The immunoprotective efficacy of Pasteurella multocida (6:B) outer membrane proteins (OMPs) was examined in the mouse model. Bacterial OMPs were extracted using sarkosyl method and analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting. Prototype vaccines were prepared using OMPs with adjuvants including dioleoyl phosphatidyl choline-based liposome and Montanide ISA206 water-in oil-in water emulsion. Antibody response to the vaccine was monitored using indirect enzyme linked immunosorbent assay. The results of the study showed that immunized mice had high titre with both the formulations. The vaccinated mice were able to survive a live virulent bacterial challenge. Based on the findings of the study it can be inferred that OMPs are important determinants of immunoprotection hence can serve as vaccine candidates against haemorrhagic septicaemia.
Basagoudanavar
S H
SH
Department of Biochemistry & Molecular Biology, Penn State College of Medicine, Hershey, PA 17033, USA.
Singh
D K
DK
Varshney
B C
BC
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D015151:Immunoblotting / Q000662:veterinary; D007928:Lethal Dose 50; D051379:Mice; D010326:Pasteurella Infections / Q000517:prevention & control / Q000662:veterinary*; D016979:Pasteurella multocida / Q000276:immunology*; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination / Q000662:veterinary
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines