title: The genetic map of finger millet, Eleusine coracana.
abstract: Restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), expressed-sequenced tag (EST), and simple sequence repeat (SSR) markers were used to generate a genetic map of the tetraploid finger millet (Eleusine coracana subsp. coracana) genome (2n = 4x = 36). Because levels of variation in finger millet are low, the map was generated in an inter-subspecific F(2) population from a cross between E. coracana subsp. coracana cv. Okhale-1 and its wild progenitor E. coracana subsp. africana acc. MD-20. Duplicated loci were used to identify homoeologous groups. Assignment of linkage groups to the A and B genome was done by comparing the hybridization patterns of probes in Okhale-1, MD-20, and Eleusine indica acc. MD-36. E. indica is the A genome donor to E. coracana. The maps span 721 cM on the A genome and 787 cM on the B genome and cover all 18 finger millet chromosomes, at least partially. To facilitate the use of marker-assisted selection in finger millet, a first set of 82 SSR markers was developed. The SSRs were identified in small-insert genomic libraries generated using methylation-sensitive restriction enzymes. Thirty-one of the SSRs were mapped. Application of the maps and markers in hybridization-based breeding programs will expedite the improvement of finger millet.
authors.affiliation: John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK.
authors.forename: Mathews M
authors.initials: MM
authors.lastname: Dida
authors.lastname: Srinivasachary
authors.forename: Sujatha
authors.initials: S
authors.lastname: Ramakrishnan
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Bennetzen
authors.forename: Mike D
authors.initials: MD
authors.lastname: Gale
authors.forename: Katrien M
authors.initials: KM
authors.lastname: Devos
mesh_terms: D002874:Chromosome Mapping; D031748:Eleusine / Q000235:genetics*; D014644:Genetic Variation