Multidimensional liquid chromatography system with an innovative solvent evaporation interface.
An orthogonal two-dimensional liquid chromatographic (2D-LC) system was developed by using a vacuum-evaporation loop-type valve interface. Normal-phase liquid chromatography (NPLC) with a bonded CN phase column was used as the first dimension, and reversed-phase liquid chromatography (RPLC) with a C(18) column was used as the second dimension. All the solvents in the loop of the interface were evaporated at 90 degrees C under vacuum conditions, leaving the analytes on the inner wall of the loop. The mobile phase of the second dimension dissolved the analytes in the loop and injected them onto the secondary column, allowing an on-line solvent exchange of a selected fraction from the first dimension to the second dimension. The chromatographic resolution of analytes on the two dimensions was maintained at their optimal condition. Sample loss due to evaporation in the interface was observed that depended on the boiling point of the compound. Separation of sixteen polycyclic aromatic hydrocarbon mixtures and a traditional Chinese medicine Angelica dahurica was demonstrated.
Tian
Hongzhe
H
Department of Instrumental and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Xu
Jing
J
Xu
Yuan
Y
Guan
Yafeng
Y
D029969:Angelica / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D003374:Coumarins / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D010936:Plant Extracts / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012997:Solvents
D003374:Coumarins; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011084:Polycyclic Aromatic Hydrocarbons; D012997:Solvents