Cloning and overexpression of ribosomal protein L39 gene from deltamethrin-resistant Culex pipiens pallens.
The complete sequence of ribosomal protein L39 has been cloned from deltamethrin-resistant strain of Culex pipiens pallens (DR1 strain). Quantitative RT-PCR analysis indicated that the RPL39 transcription level was 23.4 times higher in DR1 strain than in susceptible strain at 4th instar larvae. The RPL39 expression was also found to be consistently higher throughout the life cycle of DR1 strain. A protein of predicted size 17 kDa has been detected by Western blotting in RPL39-transfected mosquito C6/36 cells. These RPL39-transfected cells also showed enhanced deltamethrin resistance compared to plasmid vector-transfected cells as determined by methyl tritiated thymidine ((3)H-TdR) incorporation. These results indicate that RPL39 is expressed at higher levels in DR1 strain, and may confer some insecticide resistance in Cx. pipiens pallens.
Tan
Wenbin
W
Department of Pathogenic Biology, Nanjing Medical University, Nanjing, Jiangsu Province, PR China.
Sun
Lixin
L
Zhang
Donghui
D
Sun
Jing
J
Qian
Jin
J
Hu
Xiaobang
X
Wang
Weijie
W
Sun
Yan
Y
Ma
Lei
L
Zhu
Changliang
C
D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D003465:Culex / Q000737:chemistry / Q000187:drug effects / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009570:Nitriles / Q000494:pharmacology*; D010802:Phylogeny; D011722:Pyrethrins / Q000494:pharmacology*; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D014162:Transfection
D000199:Actins; D018076:DNA, Complementary; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D012269:Ribosomal Proteins; C040223:ribosomal protein L39; C017180:decamethrin; D012313:RNA