Association of HLA-A, -B, -C genes and TNF microsatellite polymorphism with psoriasis vulgaris: a study of genetic risk in Brazilian patients.
This study investigated the genetic association of HLA class I genes and TNF-alpha microsatellites. HLA-A, -B, -C typing was carried out in 92 psoriasis vulgaris patients and 160 healthy individuals using a PCR-SSP method. 70 patients and 71 controls were typed for five microsatellite polymorphisms, TNFa-e. HLA-B*13 Cw*06, HLA-B*57 Cw*06 and HLA-B*39 Cw*12 haplotypes were found to be increased in patients with psoriasis type I when compared to controls, which could determine the susceptibility to development of psoriasis. TNFa4, TNFb1, TNFe1 and TNFa2 b1 c2 d4 e1 haplotypes showed a decreased frequency (p < 0.05) in psoriasis patients when compared to controls. HLA-B*13 allele and HLA-B*13 Cw*06, TNFa11 b4 c1 d3 e3 haplotypes showed increased frequencies (p < 0.05) in patients with type II psoriasis, which suggests susceptibility to the onset of psoriasis. Our results detected polymorphisms of the HLA class I and microsatellite TNF locus which could be markers of genetic predisposition to the disease.
Biral
A C
AC
Immunogenetic Transplant Laboratory, Clinical Pathology Department, School of Medical Science, Rua Gustavo Rodrigues Dória, 255, 13083-060 Campinas, SP, Brazil.
Magalhaes
R F
RF
Wastowski
I J
IJ
Simoes
R
R
Donadi
E A
EA
Simoes
A L
AL
Mendes-Junior
C T
CT
Tanaka
A M
AM
Kraemer
M H S
MH
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001938:Brazil; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011565:Psoriasis / Q000453:epidemiology / Q000235:genetics*; D012306:Risk; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D015395:Histocompatibility Antigens Class I; D014409:Tumor Necrosis Factor-alpha