Combination of local, non-viral IL12 gene therapy and systemic paclitaxel chemotherapy in a syngeneic ID8 mouse model for human ovarian cancer.
The in vivo feasibility of the previously established ID8 and ID8-VEGF ovarian cancer models for non-viral IL-12 gene delivery by itself or in combination with paclitaxel chemotherapy, was investigated in C57BL/6 black mice. The syngeneic mouse ovarian epithelium (MOSE) cancer cell line and its more aggressive variant, a VEGF-modified strain, were used to perform these experiments. Tumor growth and survival were observed in C57/BL6 mice, inoculated with both ID8 substrains. The superiority of IL-12 gene therapy in comparison to conventional paclitaxel chemotherapy in terms of tumor size and survival was demonstrated.
Janát-Amsbury
Margit Maria
MM
Baylor College of Medicine, Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Faculty Center, Dryden Road, Suite 1143, Houston, Texas 77030, USA. amsbuy@bcm.edu
Yockman
James William
JW
Anderson
Matthew Linley
ML
Kieback
Dirk Günter
DG
Kim
Sung Wan
SW
D020533:Angiogenesis Inhibitors / Q000235:genetics / Q000627:therapeutic use*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000473:pathology / Q000628:therapy*; D017239:Paclitaxel / Q000627:therapeutic use*; D010957:Plasmids / Q000235:genetics; D011108:Polymers / Q000737:chemistry; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D020533:Angiogenesis Inhibitors; D000972:Antineoplastic Agents, Phytogenic; D011108:Polymers; D042461:Vascular Endothelial Growth Factor A; D018664:Interleukin-12; D017239:Paclitaxel