title: Investigations of the antibacterial properties of ciprofloxacin@SiO2.
abstract: The antibacterial activity of ciprofloxacin-encapsulated silica nanoshells synthesized from gold@silica core-shell nanoparticles has been investigated. The minimum inhibitory concentration of the material was found using the agar dilution method, and it showed better antibacterial activity compared to free ciprofloxacin in the case of Escherichia coli DH5, whereas the same activity was found for Lactococcus lactis MG 1363. Hydrophobicity measurements carried out in an octanol-water mixture suggested that ciprofloxacin@SiO2 is distributed almost equally in the aqueous and nonaqueous phases. The kinetics of the uptake of ciprofloxacin@SiO2 was compared with that of free ciprofloxacin. Fluorescence imaging studies carried out using fluorescein isothiocyanate@SiO2 showed that the nanoshells enter the bacterial cell. The uptake of silica shells has been probed by transmission electron microscopy also.
authors.affiliation: DST Unit on Nanoscience, Department of Chemistry and Sophisticated Analytical Instrument Facility, Indian Institute of Technology, Madras, Chennai 600 036, India.
authors.forename: M J
authors.initials: MJ
authors.lastname: Rosemary
authors.forename: Ian
authors.initials: I
authors.lastname: MacLaren
authors.forename: T
authors.initials: T
authors.lastname: Pradeep
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000737:chemistry* / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology; D007700:Kinetics; D013294:Lactococcus lactis / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D000442:Octanols / Q000737:chemistry; D015363:Quinolones / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry* / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000442:Octanols; D015363:Quinolones; D014867:Water; D002939:Ciprofloxacin; D012822:Silicon Dioxide; D016650:Fluorescein-5-isothiocyanate