SKY analysis of childhood neural tumors and cell lines demonstrates a susceptibility of aberrant chromosomes to further rearrangements.
Malignant solid tumors are commonly characterized by a large number of complex structural and numerical chromosomal alterations, which often reflect the level of genomic instability and can be associated with disease progression. The aim of this study was to evaluate whether chromosomes that harbor primary aberrations have a higher susceptibility to accumulate further alterations. We used spectral karyotyping (SKY), to compare the individual chromosomal instability of two chromosome types: chromosomes that have a primary aberration and chromosomes without an aberration, in 13 primary childhood neural tumors and seven cell lines. We found that chromosomes that contain a primary aberration are significantly (p-value<0.001) more likely to gain further structural rearrangements or to undergo numerical changes (22.6%, 36 of 159 chromosomes) than chromosomes with no initial aberration (4.9%, 54 of 1099 chromosomes). These results are highly suggestive that aberrant chromosomes in solid tumors have a higher susceptibility to accumulate further rearrangements than "normal" chromosomes.
Stanchescu
Racheli
R
Department of Pediatric Hemato-Oncology and Cancer Research Center, The Edmond and Lily Safra Children's Hospital, Sheba Medical Center, Tel-Hashomer, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
Betts
David R
DR
Yekutieli
Daniel
D
Ambros
Peter
P
Cohen
Ninette
N
Rechavi
Gideon
G
Amariglio
Ninette
N
Trakhtenbrot
Luba
L
D000782:Aneuploidy; D045744:Cell Line, Tumor; D002648:Child; D043171:Chromosomal Instability; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002877:Chromosomes, Human; D004198:Disease Susceptibility; D006801:Humans; D009423:Nervous System Neoplasms / Q000235:genetics*; D032681:Spectral Karyotyping; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured