Successful mouse cloning of an outbred strain by trichostatin A treatment after somatic nuclear transfer.
Although the somatic cloning technique has been used for numerous applications and basic research of reprogramming in various species, extremely low success rates have plagued this technique for a decade. Further in mice, the "clonable" strains have been limited to mainly hybrid F1 strains such as B6D2F1. Recently, we established a new efficient cloning technique using trichostatin A (TSA) which leads to a 2-5 fold increase in success rates for mouse cloning of B6D2F1 cumulus cells. To further test the validity of this TSA cloning technique, we tried to clone the adult ICR mouse, an outbred strain, which has never been directly cloned before. Only when TSA was used did we obtain both male and female cloned mice from cumulus and fibroblast cells of adult ICR mice with 4-5% success rates, which is comparable to 5-7% of B6D2F1. Thus, the TSA treatment is the first cloning technique to allow us to successfully clone outbred mice, demonstrating that this technique not only improves the success rates of cloning from hybrid strains, but also enables mouse cloning from normally "unclonable" strains.
Kishigami
Satoshi
S
Center for Developmental Biology, RIKEN Kobe, Japan. kishigami@cdb.riken.jp
Bui
Hong-Thuy
HT
Wakayama
Sayaka
S
Tokunaga
Kenzo
K
Van Thuan
Nguyen
N
Hikichi
Takafusa
T
Mizutani
Eiji
E
Ohta
Hiroshi
H
Suetsugu
Rinako
R
Sata
Tetsutaro
T
Wakayama
Teruhiko
T
D000818:Animals; D021461:Animals, Outbred Strains; D019976:Cloning, Organism / Q000379:methods*; D004791:Enzyme Inhibitors; D005260:Female; D005347:Fibroblasts; D006877:Hydroxamic Acids; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D053652:Nuclear Transfer Techniques; D013045:Species Specificity
D004791:Enzyme Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A