title: Delivery of DNA vaccines by agarose hydrogel implants facilitates genetic immunization in cattle.
abstract: The present study demonstrates the interest of two slow-release systems as vaccination tools in cattle. Two experiments show that a first intradermal administration of one DNA vaccine dose combined with the slow-release of a second dose conduct to a priming of the bovine herpesvirus 1-specific immune response similar to the one generated by two discrete administrations 4 weeks apart. The first experiment demonstrates the efficacy of the slow-release system with well-characterized Alzet osmotic pumps, whereas the second experiment extends the same concept with innovative agarose hydrogel implants. These latter implants are cheaper and more convenient than the osmotic pumps or repeated intradermal administrations since they contribute to an efficient priming of the immune response in a single manipulation of the animals.
authors.affiliation: Veterinary and Agrochemical Research Centre, Department of Virology, Groeselenberg 99, B-1180 Brussels, Belgium.
authors.forename: J F
authors.initials: JF
authors.lastname: Toussaint
authors.forename: A
authors.initials: A
authors.lastname: Dubois
authors.forename: M
authors.initials: M
authors.lastname: Dispas
authors.forename: D
authors.initials: D
authors.lastname: Paquet
authors.forename: C
authors.initials: C
authors.lastname: Letellier
authors.forename: P
authors.initials: P
authors.lastname: Kerkhofs
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D003692:Delayed-Action Preparations; D004058:Diffusion; D004343:Drug Implants; D005079:Excipients; D005243:Feces / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000276:immunology / Q000302:isolation & purification; D020100:Hydrogels; D007114:Immunization / Q000379:methods*; D007115:Immunization Schedule; D007249:Inflammation / Q000209:etiology / Q000473:pathology; D009500:Neutralization Tests; D009997:Osmotic Pressure; D010957:Plasmids / Q000235:genetics; D012685:Sepharose; D014611:Vaccination / Q000379:methods* / Q000662:veterinary*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000009:adverse effects; D014765:Viral Vaccines / Q000008:administration & dosage
chemical_list: D003692:Delayed-Action Preparations; D004343:Drug Implants; D005079:Excipients; D020100:Hydrogels; D019444:Vaccines, DNA; D014765:Viral Vaccines; D012685:Sepharose