title: Changes in gene expression pattern following granulocyte colony-stimulating factor administration to normal stem cell sibling donors.
abstract: Granulocyte colony-stimulating factor (G-CSF) is widely used for the mobilization of hematopoietic stem cells from normal donors. The mechanism of induced mobilization has recently been elucidated. Treatment with G-CSF is considered safe; however, long-term effects are largely unknown. The aim of this study was, therefore, to determine whether it leads to significant changes in gene expression modifications. Affymetrix Gene Chip array technology was used. Samples were collected before and at various time points (up to 9 months) after mobilization. The expression levels of 122 genes were transiently modified before and after mobilization (p < 0.05). Fifty-three genes belonging to cell growth, proliferation and communication gene ontology categories were upregulated, while 69 genes were downregulated.
  
  
  CONCLUSIONS
  The administration of G-CSF is associated with transient DNA and gene expression modifications in the lymphocytes of normal mobilized donors. A long-term follow-up of stem cell donors is recommended.
authors.affiliation: Division of Hematology, Chaim Sheba Medical Center, Tel Hashomer, Israel.
authors.forename: Ninette
authors.initials: N
authors.lastname: Amariglio
authors.forename: Jasmine
authors.initials: J
authors.lastname: Jacob-Hirsch
authors.forename: Avichai
authors.initials: A
authors.lastname: Shimoni
authors.forename: Merav
authors.initials: M
authors.lastname: Leiba
authors.forename: Gideon
authors.initials: G
authors.lastname: Rechavi
authors.forename: Arnon
authors.initials: A
authors.lastname: Nagler
mesh_terms: D015536:Down-Regulation; D005786:Gene Expression Regulation / Q000187:drug effects*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000009:adverse effects / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D035781:Siblings; D033581:Stem Cell Transplantation; D014019:Tissue Donors; D015854:Up-Regulation
chemical_list: D016179:Granulocyte Colony-Stimulating Factor