title: Quantitative analysis of representative proteome components and clustering of Helicobacter pylori clinical strains.
abstract: BACKGROUND
  Several Helicobacter pylori proteins have been reported to be associated with severe symptoms of gastric disease. However, expression levels of most of these disease-associated proteins require further evaluation in order to clarify their relationships with gastric disease patterns. Representative proteome components of 71 clinical isolates of H. pylori were analyzed quantitatively to determine whether the protein expression levels were associated with gastric diseases and to cluster clinical isolates.
  
  
  METHODS
  After two-dimensional electrophoresis (2-DE) of H. pylori isolates, spot intensities were analyzed using pdquest 2-D Gel Analysis Software. The intensities of 10 representative protein spots, identified by peptide fingerprinting using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) or peptide sequencing using quadrupole TOF MS, were subjected to the nonparametric Mann-Whitney test and hierarchical agglomerative cluster analysis. The relationship between clusters and gastric diseases was analyzed by the chi-squared test.
  
  
  RESULTS
  Although the spot intensities of the 10 representative proteins were highly variable within each gastric disease group, the expression levels of CagA, UreB, GroEL, EF-Tu, EF-P, TagD, and FldA showed some significant differences among the gastric disease patterns. On the basis of the 10 target protein intensities, hierarchical agglomerative cluster analysis generated a dendrogram with clusters indicative of chronic gastritis/gastric cancers and gastric/duodenal ulcers.
  
  
  CONCLUSIONS
  These results indicated that quantitative analysis of proteome components is a feasible method for examining disease-associated proteins and clustering clinical strains of H. pylori.
authors.affiliation: Department of Microbiology, Gyeongsang National University College of Medicine, Jinju, Gyeong-Nam, Korea.
authors.forename: Jeong-Won
authors.initials: JW
authors.lastname: Park
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.forename: Seung-Gyu
authors.initials: SG
authors.lastname: Lee
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Jun
authors.forename: Jeong-Uck
authors.initials: JU
authors.lastname: Park
authors.forename: Mi-Ja
authors.initials: MJ
authors.lastname: Chung
authors.forename: Jung-Soo
authors.initials: JS
authors.lastname: Ju
authors.forename: Damir
authors.initials: D
authors.lastname: Nizamutdinov
authors.forename: Myung-Woong
authors.initials: MW
authors.lastname: Chang
authors.forename: Hee-Shang
authors.initials: HS
authors.lastname: Youn
authors.forename: Hyung-Lyun
authors.initials: HL
authors.lastname: Kang
authors.forename: Seung-Chul
authors.initials: SC
authors.lastname: Baik
authors.forename: Woo-Kon
authors.initials: WK
authors.lastname: Lee
authors.forename: Myung-Je
authors.initials: MJ
authors.lastname: Cho
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Rhee
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D001706:Biopsy; D002908:Chronic Disease; D004381:Duodenal Ulcer / Q000150:complications / Q000382:microbiology / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005420:Flavoproteins / Q000032:analysis / Q000378:metabolism; D005756:Gastritis / Q000150:complications / Q000382:microbiology / Q000473:pathology; D006360:Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism; D016481:Helicobacter Infections / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010444:Peptide Elongation Factor Tu / Q000032:analysis / Q000378:metabolism; D010445:Peptide Elongation Factors / Q000032:analysis / Q000378:metabolism; D010544:Peroxidases / Q000032:analysis / Q000378:metabolism; D012984:Software; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000150:complications / Q000382:microbiology / Q000473:pathology; D013276:Stomach Ulcer / Q000150:complications / Q000382:microbiology / Q000473:pathology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D005420:Flavoproteins; C120194:FldA protein, Bacteria; D006360:Heat-Shock Proteins; D010445:Peptide Elongation Factors; C080866:cagA protein, Helicobacter pylori; C020746:factor EF-P; D010544:Peroxidases; D010444:Peptide Elongation Factor Tu