Human prolactin promotes human secondary immunoglobulin response in human/SCID mouse chimeras.
Recombinant human prolactin (rhPRL) was administered to huPBL-SCID mice to determine its effects on production of human immunoglobulin (Ig). The huPBL-SCID mice were injected intraperitoneally (i.p.) with 10 mug rhPRL every other day for a total of 10 injections. The results reconfirmed that rhPRL significantly increased the numbers of human CD3+ T cells and human CD19+ B cells in spleens, lymph nodes, and thymuses of huPBL-SCID mice. The huPBL-SCID mice were then concurrently given various doses of diphtheria-tetanus (DT) vaccine and 10-mug i.p. injections of rhPRL and were examined for the presence of human DT-specific proliferation of lymph node cells in vitro and antibody production in vivo. rhPRL greatly improved the engraftment of functional human lymphocytes (CD3+ T cells and CD19+ B cells) in DT-immunized huPBL-SCID mice. The rhPRL-treated, DT-immunized huPBL-SCID mice produced significantly larger amounts of DT-specific antibodies in response to the vaccine. The predominant Ig isotype induced after immunization was IgG. Thus, rhPRL stimulation promotes human secondary IgG responses in huPBL-SCID mice.
Zhang
Jian
J
Institute of Immunopharmacology & Immunotherapy, School of Pharmaceutical Sciences, Shandong University, 44 Wenhua West Road, Jinan 250012, China. zhangj65@sdu.edu.cn
Sun
Rui
R
Tian
Zhigang
Z
D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006801:Humans; D007107:Immune System / Q000187:drug effects* / Q000502:physiology; D007074:Immunoglobulin G / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D016513:Mice, SCID; D011388:Prolactin / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D007074:Immunoglobulin G; D007136:Immunoglobulins; D011994:Recombinant Proteins; D011388:Prolactin