Bacterially expressed and optimized recombinant Phl p 1 is immunobiochemically equivalent to natural Phl p 1.
Recombinant production in bacteria of soluble and monomeric Phl p 1, a major allergen of Timothy grass pollen, has proved to be very problematic. In order to facilitate expression and purification of this allergen, a recombinant variant was designed with a single amino acid substitution. Several comparative analyses with natural counterparts using electrophoretic and HPLC separations, together with immunological assays, demonstrated high equivalence. This is the first description of an approach aiming at an improvement of a natural like recombinant allergen.
Suck
Roland
R
Allergopharma Joachim Ganzer KG, Hermann-Körner-Strasse 52, 21465 Reinbek, Germany. roland.suck@allergopharma
Kamionka
Timo
T
Schäffer
Brigitte
B
Wahl
Rüdiger
R
Nandy
Andreas
A
Weber
Bernhard
B
Petersen
Arnd
A
Birner-Grünberger
Ruth
R
D V
Siva Charan
SC
Keller
Walter
W
Fiebig
Helmut
H
Cromwell
Oliver
O
D000485:Allergens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D000485:Allergens; D010940:Plant Proteins; D011994:Recombinant Proteins; C090396:PHLPI protein, Phleum pratense