[Association between calcium-sensing receptor protein expression and rat cardiomyocyte apoptosis].
OBJECTIVE
To investigate the relationship between calcium-sensing receptor protein (CaSR) expression and rat cardiomyocyte apoptosis and related signal transduction pathways.


METHODS
The CaSR, BCl2, Caspase3 protein and ERK1/2 phosphorylation or non-phosphorylation were detected by Western blot. Cardiomyocyte apoptosis was detected by flow cytometry and immunofluorescence.


RESULTS
CaSR protein was detected in rat cardiac tissue and CaSR activator gadolinium (GdCl3) induced cardiomyocyte apoptosis and increased ERK1/2 phosphorylation and expression of BCl2 and activated Caspase3. The selective mitogen-activated protein kinase (MAPK) inhibitor PD98059 abolished gadolinium -induced ERK1/2 activation and BCl2 expression, further increased the activation of Caspase3 and cardiomyocyte apoptosis.


CONCLUSIONS
Our results demonstrate the CaSR existence in cardiomyocytes and CaSR activation by gadolinium can induce myocyte apoptosis by activating Caspase3 and tyrosine protein kinase pathway.
Sun
Yi-Hua
YH
Department of Pathophysiology, Harbin Medical University, Harbin 150086, China.
Xu
Chang-qing
CQ
Li
Hong
H
Shi
Sa
S
Zhang
Wei-hua
WH
Zhao
Ya-jun
YJ
Zhang
Yan-qiao
YQ
Han
Wei-min
WM
Han
Li-ping
LP
Jiang
Chun-ming
CM
Li
Quan-feng
QF
Wang
Rui
R
D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D005260:Female; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D044169:Receptors, Calcium-Sensing / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction
D012333:RNA, Messenger; D044169:Receptors, Calcium-Sensing; D053148:Caspase 3