Association of loss of heterozygosity and PTEN gene abnormalities with paraclinical, clinical modalities and survival time of glioma patients in Malaysia.
BACKGROUND
The pattern of allelic loss of heterozygosity (LOH) and PTEN mutations appear to be associated with the progression of gliomas leading to a decrement in the survival rate of patients. This present study was carried out to determine the LOH and PTEN mutational status in glioma patients and its association with patients' survival.


METHODS
Thirty-seven Malaysian glioma patients of the Malay race were subject to PTEN mutational analysis and the presence of LOH using the cold single-strand conformation polymorphism method, and their clinical and paraclinical response were correlated.


RESULTS
Among analysed glioma patients, seven (21.6%) cases with PTEN mutations were detected and 12 (32.4%) of 37 patients showed presence of LOH. Univariate analysis showed that tumour grade, vascularization, PTEN mutation, LOH and combination of both PTEN mutation and LOH were significantly associated with glioma patients' survival. Multivariate analysis revealed that no factors contributed to survival time.


CONCLUSIONS
The results show that PTEN mutation and LOH are quite frequent in Malaysian glioma patients. However, they have no impact on the survival outcome of patients.
Abdullah
Jafri Malin
JM
Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia. deptneurosciencesppspusm@yahoo.com
Farizan
Ahmad
A
Asmarina
Ku
K
Zainuddin
Norafiza
N
Ghazali
Mazira Mohamad
MM
Jaafar
Hasnan
H
Isa
Mohd Nizam
MN
Naing
Nyi Nyi
NN
D000328:Adult; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D003627:Data Interpretation, Statistical; D005260:Female; D005910:Glioma / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D006579:Heterozygote; D006801:Humans; D008296:Malaysia; D008297:Male; D015999:Multivariate Analysis; D009154:Mutation; D009367:Neoplasm Staging; D051059:PTEN Phosphohydrolase; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis; D012044:Regression Analysis; D016019:Survival Analysis
D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human