Segmentation of bone CT images and assessment of bone structure using measures of complexity.
A nondestructive and noninvasive method for numeric characterization (quantification) of the structural composition of human bone tissue has been developed and tested. In order to quantify and to compare the structural composition of bones from 2D computed tomography (CT) images acquired at different skeletal locations, a series of robust, versatile, and adjustable image segmentation and structure assessment algorithms were developed. The segmentation technique facilitates separation from cortical bone and standardizes the region of interest. The segmented images were symbol-encoded and different aspects of the bone structural composition were quantified using six different measures of complexity. These structural examinations were performed on CT images of bone specimens obtained at the distal radius, humeral mid-diaphysis, vertebral body, femoral head, femoral neck, proximal tibia, and calcaneus. In addition, the ability of the noninvasive and nondestructive measures of complexity to quantify trabecular bone structure was verified by comparing them to conventional static histomorphometry performed on human fourth lumbar vertebral bodies. Strong correlations were established between the measures of complexity and the histomorphometric parameters except for measures expressing trabecular thickness. Furthermore, the ability of the measures of complexity to predict vertebral bone strength was investigated by comparing the outcome of the complexity analysis of the CT images with the results of a biomechanical compression test of the third lumbar vertebral bodies from the same population as used for histomorphometry. A multiple regression analysis using the proposed measures including structure complexity index, structure disorder index, trabecular network index, index of a global ensemble, maximal L-block, and entropy of x-ray attenuation distribution revealed an excellent relationship (r=0.959, r2=0.92) between the measures of complexity and compressive bone strength. In conclusion, the image segmentation techniques and the assessment of bone architecture by measures of complexity have been successfully applied to analyze high-resolution peripheral quantitative computed tomography (pQCT) and CT images obtained from the distal radius, humeral mid-diaphysis, third and fourth lumbar vertebral bodies, proximal femur, proximal tibia, and calcaneus. The proposed approach is of broad interest as it can be applied for the quantification of structures and textures originating from different imaging modalities in other fields of science.
Saparin
Peter
P
Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Research Campus Golm, 14424 Potsdam, Germany. peter.saparin@mpikg.mpg.de
Thomsen
Jesper Skovhus
JS
Kurths
Jürgen
J
Beller
Gisela
G
Gowin
Wolfgang
W
D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D001842:Bone and Bones / Q000473:pathology*; D002138:Calibration; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008297:Male; D008875:Middle Aged; D013131:Spine / Q000473:pathology; D013977:Tibia / Q000000981:diagnostic imaging / Q000473:pathology*; D014057:Tomography, X-Ray Computed / Q000379:methods*; D014965:X-Rays