Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis.
Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).
Chantratita
Narisara
N
Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. narisara@tropmedres.ac
Wuthiekanun
Vanaporn
V
Thanwisai
Aunchalee
A
Limmathurotsakul
Direk
D
Cheng
Allen C
AC
Chierakul
Wirongrong
W
Day
Nicholas P J
NP
Peacock
Sharon J
SJ
D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D016957:Burkholderia pseudomallei / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests; D008554:Melioidosis / Q000175:diagnosis* / Q000453:epidemiology; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial