Enhancement of polysaccharides production in Ganoderma lucidum by the addition of ethyl acetate extracts from Eupolyphaga sinensis and Catharsius molossus.
To screen stimulators from Chinese medicinal insects for mycelial growth and polysaccharides production of Ganoderma lucidum, G. lucidum was inoculated into the media with and without supplementation of medicinal insect extracts. The ethyl acetate extract of Eupolyphaga sinensis at 55 mg l(-1) lead to significant increase in both biomass and intracellular polysaccharides (IPS) concentration from 8.53 +/- 0.41 to 14.16 +/- 0.43 and 1.28 +/- 0.09 to 2.13 +/- 0.11 g l(-1), respectively. In addition, the ethyl acetate extract of Catharsius molossus at 55 mg l(-1) significantly enhanced extracellular polysaccharides (EPS) production; the EPS yield increased from 350.9 +/- 14.1 to 475.1 +/- 15.3 mg l(-1). There were no new components in the two types of polysaccharides obtained by the addition of the insect extracts.
Liu
Gao-Qiang
GQ
Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, 170 Huihe Road, Wuxi, 214036, People's Republic of China.
Zhang
Ke-Chang
KC
D000818:Animals; D018533:Biomass; D002457:Cell Extracts / Q000494:pharmacology*; D003058:Cockroaches / Q000737:chemistry*; D001517:Coleoptera / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D011134:Polysaccharides / Q000096:biosynthesis*; D020940:Reishi / Q000254:growth & development / Q000378:metabolism*
D002457:Cell Extracts; D003470:Culture Media; D011134:Polysaccharides