title: Mesenchymal stem cell ingrowth and differentiation on coralline hydroxyapatite scaffolds.
abstract: Culture of osteogenic cells on a porous scaffold could offer a new solution to bone grafting using autologous human mesenchymal stem cells (hMSC) from the patient. We compared coralline hydroxyapatite scaffolds with pore sizes of 200 and 500 microm for expansion and differentiation of hMSCs. We cultivated the hMSC statically or in spinner flasks for 1, 7, 14 and 21 days and found that the 200-microm pore scaffolds exhibited a faster rate of osteogenic differentiation than did the 500-microm pore scaffolds as shown by an alkaline phosphatase activity assay and real-time reverse transcriptase polymerase chain reaction for 10 osteogenic markers. The 500-microm scaffolds had increased proliferation rates and accommodated a higher number of cells (shown by DNA content, scanning electron microscopy and fluorescence microscopy). Thus the porosity of a 3D microporous biomaterial may be used to steer hMSC in a particular direction. We found that dynamic spinner flask cultivation of hMSC/scaffold constructs resulted in increased proliferation, differentiation and distribution of cells in scaffolds. Therefore, spinner flask cultivation is an easy-to-use inexpensive system for cultivating hMSCs on small to intermediate size 3D scaffolds.
authors.affiliation: Orthopedic Research Laboratory, Laboratory for Molecular Orthopedics, Clinical Institute, Aarhus University Hospital, Norrebrogade 44 bldg 1A, DK-8000 Aarhus C, Denmark. tina.mygind@ki.au.dk
authors.forename: Tina
authors.initials: T
authors.lastname: Mygind
authors.forename: Maik
authors.initials: M
authors.lastname: Stiehler
authors.forename: Anette
authors.initials: A
authors.lastname: Baatrup
authors.forename: Haisheng
authors.initials: H
authors.lastname: Li
authors.forename: Xuenong
authors.initials: X
authors.lastname: Zou
authors.forename: Allan
authors.initials: A
authors.lastname: Flyvbjerg
authors.forename: Moustapha
authors.initials: M
authors.lastname: Kassem
authors.forename: Cody
authors.initials: C
authors.lastname: Bünger
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002516:Ceramics / Q000737:chemistry*; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D016348:Osseointegration / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D006882:Hydroxyapatites; C119955:coralline hydroxyapatite