Mechanisms of guanosine triphosphate hydrolysis by Ras and Ras-GAP proteins as rationalized by ab initio QM/MM simulations.
The hydrolysis reaction of guanosine triphosphate (GTP) by p21(ras) (Ras) has been modeled by using the ab initio type quantum mechanical-molecular mechanical simulations. Initial geometry configurations have been prompted by atomic coordinates of the crystal structure (PDBID: 1QRA) corresponding to the prehydrolysis state of Ras in complex with GTP. Multiple searches of minimum energy geometry configurations consistent with the hydrogen bond networks have been performed, resulting in a series of stationary points on the potential energy surface for reaction intermediates and transition states. It is shown that the minimum energy reaction path is consistent with an assumption of a two-step mechanism of GTP hydrolysis. At the first stage, a unified action of the nearest residues of Ras and the nearest water molecules results in a substantial spatial separation of the gamma-phosphate group of GTP from the rest of the molecule (GDP). This phase of hydrolysis process proceeds through the low barrier (16.7 kcal/mol) transition state TS1. At the second stage, the inorganic phosphate is formed in consequence of proton transfers mediated by two water molecules and assisted by the Gln61 residue from Ras. The highest transition state at this segment, TS3, is estimated to have an energy 7.5 kcal/mol above the enzyme-substrate complex. The results of simulations are compared to the previous findings for the GTP hydrolysis in the Ras-GAP (p21(ras)-p120(GAP)) protein complex. Conclusions of the modeling lead to a better understanding of the anticatalytic effect of cancer causing mutation of Gln61 from Ras, which has been debated in recent years.
Grigorenko
Bella L
BL
Department of Chemistry, M.V. Lomonosov Moscow State University, Moscow, 119992, Russian Federation.
Nemukhin
Alexander V
AV
Shadrina
Maria S
MS
Topol
Igor A
IA
Burt
Stanley K
SK
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001665:Binding Sites; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D011905:Genes, ras; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006801:Humans; D006860:Hydrogen Bonding; D006868:Hydrolysis; D008956:Models, Chemical; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D017354:Point Mutation; D011485:Protein Binding; D011487:Protein Conformation; D016283:Proto-Oncogene Proteins p21(ras) / Q000737:chemistry / Q000378:metabolism*; D011789:Quantum Theory; D014867:Water; D020703:ras GTPase-Activating Proteins / Q000737:chemistry / Q000378:metabolism*
D020703:ras GTPase-Activating Proteins; D014867:Water; D006160:Guanosine Triphosphate; D016283:Proto-Oncogene Proteins p21(ras)