title: Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip.
abstract: Low volume (LV) amplification (1 microL) of nuclear DNA (nucDNA) on a chemically structured chip is an appropriate and highly sensitive method to simultaneously amplify amelogenin and 15 forensically relevant short tandem repeats (STR). In this study, a combined method using on-chip LV amplification of mitochondrial DNA (mtDNA) and subsequent on-chip LV cycle sequencing was established to obtain a method, which is sensitive and robust enough to allow reliable analysis of DNA amounts representing the single cell level. All the necessary steps of the procedure--except for the purification of the sequencing products--were accomplished within the same final 2-microL reaction volume.
authors.affiliation: Institute of Legal Medicine, Albert Ludwig University Freiburg, Albertstrasse 9, 79104, Freiburg, Germany. sabine.lutz-bonengel@uniklinik-freiburg.de
authors.forename: Sabine
authors.initials: S
authors.lastname: Lutz-Bonengel
authors.forename: Timo
authors.initials: T
authors.lastname: Sänger
authors.forename: Marielle
authors.initials: M
authors.lastname: Heinrich
authors.forename: Ulrike
authors.initials: U
authors.lastname: Schön
authors.forename: Ulrike
authors.initials: U
authors.lastname: Schmidt
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics; D046209:Electrophoresis, Microchip / Q000379:methods*; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004272:DNA, Mitochondrial