title: Isolation of a soil bacterium capable of biodegradation and detoxification of endosulfan and endosulfan sulfate.
abstract: Endosulfan, an endocrine disrupting chemical, is a widely used cyclodiene organochlorine pesticide worldwide, and it blocks neuronal GABA(A)-gated chloride channels in mammals and aquatic organisms. Endosulfan and its metabolites, such as endosulfan sulfate, are persistent in environments and are considered as toxic chemicals. For bioremediation of endosulfan, in this study, an attempt was made to isolate an endosulfan and endosulfan sulfate degrading bacterium from endosulfan-polluted agricultural soil. Through repetitive enrichment and successive subculture using endosulfan or endosulfan sulfate as the sole carbon source, a bacterium KS-2P was isolated. The KS-2P was identified as Pseudomonas sp. on the basis of the results of a 16S rDNA sequencing analysis and MIDI test. The degradation ratios for endosulfan or endosulfan sulfate in minimal medium containing endosulfan (23.5 microg mL(-1)) or endosulfan sulfate (21 microg mL(-1)) were 52% and 71%, respectively. Our results suggest that Pseudomonas sp. KS-2P has potential as a biocatalyst for endosulfan bioremediation.
authors.affiliation: School of Bioresource Sciences, Andong National University, Andong 760-749, South Korea.
authors.forename: Jung-Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Ho-Yong
authors.initials: HY
authors.lastname: Sohn
authors.forename: Kee-Sun
authors.initials: KS
authors.lastname: Shin
authors.forename: Min-Sub
authors.initials: MS
authors.lastname: Jo
authors.forename: Jang-Eok
authors.initials: JE
authors.lastname: Kim
authors.forename: Se-Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Shin
authors.forename: Eun-Joo
authors.initials: EJ
authors.lastname: Kum
authors.forename: Gi-Seok
authors.initials: GS
authors.lastname: Kwon
mesh_terms: D001673:Biodegradation, Environmental; D004726:Endosulfan / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D007306:Insecticides; D004726:Endosulfan