title: Application of precolumn oxidation HPLC method with fluorescence detection to evaluate saxitoxin levels in discrete brain regions of rats.
abstract: Saxitoxin (STX) is one of several related toxins that cause paralytic shellfish poisoning (PSP). This toxin blocks neuronal transmission by binding to the voltage-gated Na+ channel and for this reason, it has been widely used in the study of Na+ channel. The aim of this study was to analyze STX distribution in different rat brain regions after its acute intraperitoneal (i.p.) administration. Male rats (150-200 g) were injected i.p. with STX (5 and 10 microg/kg of body weight). After three time intervals of 30, 60, and 120 min (for 5 microg/kg STX dose) and 30 min (for 10 microg/kg STX dose) animals were sacrificed by cervical dislocation. Brains were removed and dissected in seven regions. STX concentration was measured using a precolumn oxidation high-performance liquid chromatographic method with fluorescence detection (HPLC/FLD). STX was found in all the regions evaluated at ppm levels meaning that STX peripherical administered across the blood-brain barrier and is distributed along the whole brain.
authors.affiliation: Departamento de Ciencias Marinas, Instituto Tecnológico de Boca del Río, Ver. México. rosynac@uvigo.es
authors.forename: Rosa Carmina
authors.initials: RC
authors.lastname: Cervantes Cianca
authors.forename: Miguel Alfonso
authors.initials: MA
authors.lastname: Pallares
authors.forename: Rafael
authors.initials: R
authors.lastname: Durán Barbosa
authors.forename: Lucía
authors.initials: L
authors.lastname: Vidal Adan
authors.forename: J Manuel
authors.initials: JM
authors.lastname: Leão Martins
authors.forename: Ana
authors.initials: A
authors.lastname: Gago-Martínez
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004141:Dinoflagellida; D007274:Injections, Intraperitoneal; D008297:Male; D010084:Oxidation-Reduction; D011042:Poisons / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D011237:Predictive Value of Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D012530:Saxitoxin / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D012758:Shellfish; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution
chemical_list: D011042:Poisons; D012530:Saxitoxin