title: Role of cysteine 341 and arginine 348 of GLP-1 receptor in G-protein coupling.
abstract: We have demonstrated the ability of peptides derived from the third intracellular loop of GLP-1 receptor to differently modulate activity of four different types of G-proteins overexpressed in sf9 cells. In this respect, the involvement of Cys(341) in inhibition of G(s) and Cys(341) in activation of G(s) and in inhibition of G(i1,) G(o), and G(11), respectively, indicates their potential role in discrimination between different types of G-proteins. Moreover, these two amino acids from the third intracellular loop might represent an important novel targets for covalent modification by downstream regulators in signaling through GLP-1 receptor.
authors.affiliation: Institute of Biochemistry, Medical Faculty, University of Ljubljana, Vrazov trg 2, 1000, Ljubljana, Slovenia. aljosa.bavec@mf.uni-lj.si
authors.forename: Aljosa
authors.initials: A
authors.lastname: Bavec
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiang
authors.forename: Ulo
authors.initials: U
authors.lastname: Langel
authors.forename: Matjaz
authors.initials: M
authors.lastname: Zorko
mesh_terms: D000818:Animals; D001120:Arginine / Q000502:physiology*; D002417:Cattle; D002478:Cells, Cultured; D003545:Cysteine / Q000502:physiology*; D000067757:Glucagon-Like Peptide-1 Receptor; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D018027:Receptors, Glucagon / Q000737:chemistry* / Q000378:metabolism* / Q000502:physiology; D011994:Recombinant Proteins / Q000235:genetics; D015398:Signal Transduction; D018411:Spodoptera
chemical_list: D000067757:Glucagon-Like Peptide-1 Receptor; D018027:Receptors, Glucagon; D011994:Recombinant Proteins; D001120:Arginine; D020962:Heterotrimeric GTP-Binding Proteins; D003545:Cysteine