title: [IL-10 gene modification on immature dendritic cells induces antigen-specific tolerance in experimental autoimmune myocarditis].
abstract: OBJECTIVE
  To investigate whether IL-10 gene modification on immature dendritic cells (iDC) could induce autoimmune tolerance in rat experimental autoimmune myocarditis (EAM).
  
  
  METHODS
  EAM was induced by cardiac myosin immunization on day 0 and day 7 in rats. A total of 2 x 10(6) mature DC (mDC), iDC, pcDNA3 transfected iDC, pcDNA3-IL-10 transfected iDC or PBS were injected intravenously at 5th immunization day. Three weeks later, echocardiography and HE staining were performed to observe the cardiac function and myocardial inflammation. Th1/Th2 cytokines were detected by ELISA and MHC-II molecules, costimulatory molecules were identified by flow cytometry. In vitro T lymphocyte proliferation assay and adoptive transfer of DCs were performed to determine the antigen specific tolerance induced by IL-10 gene modification on iDCs.
  
  
  RESULTS
  EAM rats treated with pcDNA3-IL-10 transfected iDC showed improved cardiac function and reduced inflammatory cells infiltration into myocardium. Moreover, lower Th1 and higher Th2-type response was induced, MHC-II and costimulatory molecules down-regulated and antigen specific immunological responses towards cardiac myosin inhibited in pcDNA3-IL-10-iDC treated EAM rats.
  
  
  CONCLUSIONS
  Treatment with IL-10 gene modified iDCs could ameliorates EAM by inducing Th2 polarization and down-regulation of MHC-II molecules and costimulatory molecule expressions.
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Cheng
authors.initials: C
authors.lastname: Gao
authors.forename: Bao-Guo
authors.initials: BG
authors.lastname: Zhou
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Rui-Hong
authors.initials: RH
authors.lastname: Zhang
authors.forename: Yi-Hui
authors.initials: YH
authors.lastname: Kong
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Run-Tao
authors.initials: RT
authors.lastname: Gan
authors.forename: Hong-Jie
authors.initials: HJ
authors.lastname: Xue
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Geng
authors.forename: Shu-Sen
authors.initials: SS
authors.lastname: Yang
authors.forename: Qun
authors.initials: Q
authors.lastname: Shao
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001327:Autoimmune Diseases / Q000276:immunology*; D001854:Bone Marrow Cells; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology*; D015316:Genetic Therapy; D007108:Immune Tolerance; D016753:Interleukin-10 / Q000235:genetics* / Q000276:immunology; D009205:Myocarditis / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D016753:Interleukin-10