[Comparative chemical proteomics: simultaneous identification of disease-specific protein targets and their small molecule-binding partners, suitable as drug candidates].
The simultaneous identification of disease-specific protein targets and their small molecule binding partners, suitable as drug candidates, could radically reduce the timeline and costs of drug discovery and development. Comparative chemical proteomics provides a novel approach to achieve this goal through rapid detection of overexpressed proteins in diseased samples by the application of small molecule microarrays. The interacting small molecules enables direct affinity-based isolation and identification of the proteins. In the present paper we report comparative chemical proteomics studies on melanocytes and melanoma cell-lines, which led to the identification of 3 overexpressed proteins (e.g. -tubulin) together with their small molecule binding partner.
Dormán
György
G
ComGenex, Budapest, Záhony.
Puskás
László G
LG
Fehér
Liliána Z
LZ
Hackler
László
L
Lorincz
Zsolt
Z
Lang
Csaba
C
Urge
László
L
Darvas
Ferenc
F
D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D002625:Chemistry, Organic / Q000379:methods; D006801:Humans; D008544:Melanocytes; D008545:Melanoma; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
D002352:Carrier Proteins; D009363:Neoplasm Proteins; D011506:Proteins; D014404:Tubulin