Chemico-enzymatic synthesis of a new fluorescent-labeled DNA by PCR with a thymidine nucleotide analogue bearing an acridone derivative.
Triphosphate of a thymidine analogue bearing acridone was prepared and incorporated as a substrate for PCR using KOD Dash DNA polymerase forming a new fluorescent-labeled DNA that is useful as a DNA probe.
Shoji
Atsushi
A
Department of Applied Chemistry, Gunma University, Kiryu, Gunma 376-8515, Japan.
Hasegawa
Tomoya
T
Kuwahara
Masayasu
M
Ozaki
Hiroaki
H
Sawai
Hiroaki
H
D000166:Acridines / Q000138:chemical synthesis*; D004247:DNA / Q000138:chemical synthesis* / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D009711:Nucleotides / Q000138:chemical synthesis*; D010777:Photochemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet; D013936:Thymidine / Q000031:analogs & derivatives*
D000166:Acridines; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009711:Nucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D013936:Thymidine