An isocratic liquid chromatography method for determining HIV non-nucleoside reverse transcriptase inhibitor and protease inhibitor concentrations in human plasma.
An efficient, isocratic high performance liquid chromatography (HPLC) method for determining human immunodeficiency virus (HIV) non-nucleoside reverse transcriptase inhibitors (NNRTIs) and protease inhibitors (PIs) in plasma is advantageous for laboratories participating in clinical trials and therapeutic drug monitoring (TDM) programs, or conducting small animal research. The combination of isocratic reversed phase chromatography using an S-3, 3.0 mm x 150 mm column along with low plasma volume (200 microl), rapid liquid-liquid extraction, and detection at a single wavelength (212 nm) over a short run time makes this method valuable. Within and between assay variability ranges from 0.8 to 3.5% and 1.2-6.2%, respectively. Accuracy ranges from 91.0 to 112.8% for four quality controls (50, 100, 1000, and 10,000 ng/ml) for all drugs measured (efavirenz, nevirapine, amprenavir, atazanavir, indinavir, lopinavir, nelfinavir, ritonavir, and saquinavir).
Weller
Dennis R
DR
Department of Laboratory Medicine and Pathology, Medical School, University of Minnesota, 420 Delaware St. SE, Minneapolis, MN 55455, USA.
Brundage
Richard C
RC
Balfour
Henry H
HH
Vezina
Heather E
HE
D000480:Alkynes; D000069446:Atazanavir Sulfate; D048588:Benzoxazines / Q000097:blood; D002219:Carbamates / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003521:Cyclopropanes; D005663:Furans; D017320:HIV Protease Inhibitors / Q000097:blood*; D006801:Humans; D019469:Indinavir / Q000097:blood; D061466:Lopinavir; D019888:Nelfinavir / Q000097:blood; D019829:Nevirapine / Q000097:blood; D009842:Oligopeptides / Q000097:blood; D011725:Pyridines / Q000097:blood; D011744:Pyrimidinones / Q000097:blood; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000097:blood*; D019438:Ritonavir / Q000097:blood; D019258:Saquinavir / Q000097:blood; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013449:Sulfonamides / Q000097:blood
D000480:Alkynes; D048588:Benzoxazines; D002219:Carbamates; D003521:Cyclopropanes; D005663:Furans; D017320:HIV Protease Inhibitors; D009842:Oligopeptides; D011725:Pyridines; D011744:Pyrimidinones; D018894:Reverse Transcriptase Inhibitors; D013449:Sulfonamides; D061466:Lopinavir; D000069446:Atazanavir Sulfate; C095108:amprenavir; D019469:Indinavir; D019829:Nevirapine; D019888:Nelfinavir; C098320:efavirenz; D019258:Saquinavir; D019438:Ritonavir