[The characterization of Th1/Th2 profile in end-stage renal disease patients and the correlation with the apoptosis of T lymphocyte].
OBJECTIVE
To investigate the characterization of Th1/Th2 profile in patients with end-stage renal disease (ESRD) and the correlation with the apoptosis of the peripheral blood T cells; and to study the influence of different dialysis membranes on the apoptosis of T lymphocyte.


METHODS
T cells from 10 non-dialyzed (ND) patients, 45 maintenance hemodialysis patients with cellulose acetate (CA) membrane, low-flux (PS-LF) and high-flux polusulfone (PS-HF) membrane, and 8 healthy volunteers (HC) were separated and stimulated with PHA for 24 hours in vitro. Then the apoptosis of T cells and supernatants levels of IFN-gamma and IL-4 were detected by Flow cytometry (FCM) and ELISA.


RESULTS
In ESRD patients, the expression of Annexin V in T lymphocyte was higher than that of group HC (P<0.05), group CA was higher than group PS-HF and PS-LF (P<0.05). The level of IFN-gamma of ESRD patients was decreased significant compared with that in group HC (P<0.05), and there was negative correlation. between the Annexin V and IFN-gamma. IL-4 was increased in ESRD patients (P<0.05) and it was positive correlated with Annexin V.


CONCLUSIONS
ESRD patients showed suppressed secretion of IFN-gamma, increased secretion of IL-4 and apoptosis of T lymphocytes.
Xie
De-Qiong
DQ
Department of Nephrology, First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China.
Gan
Hua
H
Du
Xiao-Gang
XG
Li
Zheng-Rong
ZR
Wu
Jiang
J
D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D016022:Case-Control Studies; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000097:blood / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D001613:beta 2-Microglobulin / Q000097:blood
D017304:Annexin A5; D001613:beta 2-Microglobulin; D015847:Interleukin-4; D007371:Interferon-gamma