Potato tuber proteomics: comparison of two complementary extraction methods designed for 2-DE of acidic proteins.
Two protein extraction procedures were tested in order to remove interfering compounds prior to 2-DE of potato tubers. These methods using SDS lysis buffer and phenol-phase extraction were compared regarding the quality of the resulting 2-D gel. While the resolution of SDS extracts on semipreparative gels seems better, both methods lead to similar extraction yields and total number of spots. The procedures are complementary regarding the Mr range of preferentially extracted proteins.
Delaplace
Pierre
P
Plant Biology Unit, Gembloux Agricultural University, Gembloux, Belgium. delaplace.p@fsagx.ac.be
van der Wal
Froukje
F
Dierick
Jean-François
JF
Cordewener
Jan H G
JH
Fauconnier
Marie-Laure
ML
du Jardin
Patrick
P
America
Antoine H P
AH
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D019800:Phenol; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D035281:Plant Tubers / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012967:Sodium Dodecyl Sulfate; D011198:Solanum tuberosum / Q000737:chemistry*
D010940:Plant Proteins; D019800:Phenol; D012967:Sodium Dodecyl Sulfate