[Density expression of the CD20 antigen on population of tumor cells in patients with chronic B-lymphocyte lymphoproliferative diseases].
BACKGROUND
Rituximab is being used successfully in the treatment of patients with chronic B-cell lymphoproliferative diseases. The success of treatment by rituximab is influenced, among other factors, by the antigen density on tumor cells. Therefore, the authors analyzed and compared the densities of the CD20 antigen in patients with chronic lymphoproliferative diseases.


RESULTS
Previously untreated patients with B-chronic lymphocytic leukemia (B-CLL), mantle-cell lymphoma (MCL), and small-cell lymphocytic lymphoma (SCLL) were evaluated by flow cytometry. The control group consisted of blood donors. The CD20 density was measured on tumor cell populations in patients and on the B-lymphocytes of the control group. The density was expressed in MESE In the patients with B-CLL and SCLL, the CD20 density was low (25,300 vs. 36,100 MESF) and it was significantly lower than in donors (172,800 MESF; p<0.001). The difference between B-CLL and SCLL patients was not statistically significant. The density in MCL patients (196,300 MESF) was comparable to that of donors.


CONCLUSIONS
We did not prove statistical different density of CD20 antigen in patients with SCLL when compared with B-CLL patients. High density in MCL patients may be helpful in differential diagnosis against B-CLL and
Suková
V
V
Laborator flow cytometrie a celulární terapie LF MU a FN, Brno. 43047@mail.muni.cz
Klabusay
M
M
Coupek
P
P
Brychtová
Y
Y
Doubek
M
M
Mayer
J
J
D000368:Aged; D018951:Antigens, CD20 / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015448:Leukemia, B-Cell / Q000276:immunology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology; D016393:Lymphoma, B-Cell / Q000276:immunology*; D020522:Lymphoma, Mantle-Cell / Q000276:immunology; D008297:Male; D008875:Middle Aged
D018951:Antigens, CD20