Nucleocapsid amino acids 211 to 254, in particular, tetrad glutamines, are essential for the interaction between the nucleocapsid and membrane proteins of SARS-associated coronavirus.
GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N) interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted.
Fang
Xiaonan
X
State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, PR China.
Ye
Lin-Bai
LB
Zhang
Yijuan
Y
Li
Baozong
B
Li
Shanshan
S
Kong
Lingbao
L
Wang
Yuhua
Y
Zheng
Hong
H
Wang
Wei
W
Wu
Zhenghui
Z
D019943:Amino Acid Substitution / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D000086462:Coronavirus Nucleocapsid Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D018698:Glutamic Acid / Q000235:genetics / Q000378:metabolism; D005973:Glutamine / Q000235:genetics / Q000378:metabolism*; D019251:Nucleocapsid / Q000378:metabolism*; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000378:metabolism*
D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; D010455:Peptides; D005973:Glutamine; C097188:polyglutamine; D018698:Glutamic Acid