Effects of raloxifene on caveolin-1 mRNA and protein expressions in vascular smooth muscle cells.
Caveolin-1 is regulated by estrogen in vascular smooth muscle cells. Raloxifene, a selective estrogen receptor modulator that possibly has cardioprotective properties without an increased risk of cancer or other side effects of estrogen, may be used in women with risk of coronary artery disease. However, the relationship between raloxifene and caveolin-1 is still unknown. Therefore, this study was designed to see whether raloxifene regulates caveolin-1 expression and if so, whether such regulation is mediated by estrogen receptor. Rat aortic smooth muscle cells were cultured in the absence or presence of raloxifene (10(8-) to 10(6-) M) for 12 or 24 h. Both mRNA and protein levels of caveolin-1 were increased significantly after 24 h treatment with raloxifene. These increases were inhibited by estrogen receptor antagonist ICI 182780 (10(5-) M). Results of this study suggest that raloxifene stimulates caveolin-1 transcription and translation through estrogen receptor mediated mechanisms.
Yang
Fa-Lin
FL
Institute of Biochemistry and Molecular Biology, Medical College, Shandong University, Jinan 250012, China. falin34@yahoo.com.cn
He
Hong
H
Liu
Xian-Xi
XX
Tu
Bing
B
Zeng
Xian-Wei
XW
Su
Ji-Xin
JX
Wang
Xin
X
Hu
Qin
Q
D000818:Animals; D001011:Aorta / Q000166:cytology; D002316:Cardiotonic Agents / Q000494:pharmacology*; D051242:Caveolin 1 / Q000096:biosynthesis*; D002478:Cells, Cultured; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D000077267:Fulvestrant; D005786:Gene Expression Regulation; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D020849:Raloxifene Hydrochloride / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011960:Receptors, Estrogen / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology; D020845:Selective Estrogen Receptor Modulators / Q000494:pharmacology*
D002316:Cardiotonic Agents; D051242:Caveolin 1; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D020845:Selective Estrogen Receptor Modulators; D000077267:Fulvestrant; D020849:Raloxifene Hydrochloride; D004958:Estradiol