title: HIV induces maturation of monocyte-derived dendritic cells and Langerhans cells.
abstract: In HIV infection, dendritic cells (DCs) may play multiple roles, probably including initial HIV uptake in the anogenital mucosa, transport to lymph nodes, and subsequent transfer to T cells. The effects of HIV-1 on DC maturation are controversial, with several recent conflicting reports in the literature. In this study, microarray studies, confirmed by real-time PCR, demonstrated that the genes encoding DC surface maturation markers were among the most differentially expressed in monocyte-derived dendritic cells (MDDCs), derived from human blood, treated with live or aldrithriol-2-inactivated HIV-1(BaL). These effects translated to enhanced cell surface expression of these proteins but differential expression of maturation markers was only partial compared with the effects of a conventional potent maturation stimulus. Such partially mature MDDCs can be converted to fully mature cells by this same potent stimulus. Furthermore, live HIV-1 stimulated greater changes in maturation marker surface expression than aldrithriol-2-inactivated HIV-1 and this enhanced stimulation by live HIV-1 was mediated via CCR5, thus suggesting both viral replication-dependent and -independent mechanisms. These partially mature MDDCs demonstrated enhanced CCR7-mediated migration and are also able to stimulate interacting T cells in a MLR, suggesting DCs harboring HIV-1 might prepare CD4 lymphocytes for transfer of HIV-1. Increased maturation marker surface expression was also demonstrated in native DCs, ex vivo Langerhans cells derived from human skin. Thus, HIV initiates maturation of DCs which could facilitate subsequent enhanced transfer to T cells.
authors.affiliation: Centre for Virus Research, Westmead Millennium Institute, Sydney, Australia.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Harman
authors.forename: John
authors.initials: J
authors.lastname: Wilkinson
authors.forename: Chris R
authors.initials: CR
authors.lastname: Bye
authors.forename: Lidija
authors.initials: L
authors.lastname: Bosnjak
authors.forename: J Lewis
authors.initials: JL
authors.lastname: Stern
authors.forename: Monique
authors.initials: M
authors.lastname: Nicholle
authors.forename: Joey
authors.initials: J
authors.lastname: Lai
authors.forename: Anthony L
authors.initials: AL
authors.lastname: Cunningham
mesh_terms: D019138:Antibodies, Blocking / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D054421:Chemokine CCL21; D019742:Chemokines, CC / Q000737:chemistry / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D005819:Genetic Markers; D006131:Growth Inhibitors / Q000502:physiology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007801:Langerhans Cells / Q000166:cytology* / Q000821:virology*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D009000:Monocytes / Q000276:immunology / Q000821:virology; D019713:Receptors, CCR5 / Q000276:immunology; D014775:Virus Activation / Q000235:genetics / Q000276:immunology
chemical_list: D019138:Antibodies, Blocking; C515478:CCL21 protein, human; D054421:Chemokine CCL21; D019742:Chemokines, CC; D005819:Genetic Markers; D006131:Growth Inhibitors; D008565:Membrane Proteins; D019713:Receptors, CCR5