Attenuation of ischemia-reperfusion injury by ascorbic acid in the canine renal transplantation.
This study examined the effects of ascorbic acid on the attenuation of an ischemia-reperfusion (I/R) injury after a canine renal transplantation. Eight beagle dogs were subjected to a renal auto-transplantation followed by the administration of ascorbic acid (treatment group) and the same amount of vehicle (physiological saline, control group). Blood samples were collected from these dogs to perform the kidney function tests and the invasive blood pressure was measured in the renal artery at pre- and post-anastomosis. The antioxidant enzymes of level 72 h after the transplant were measured. The kidneys were taken for a histopathology evaluation at day 21. The kidney function tests showed a significant difference between the control and treatment group. The invasive blood pressure in the renal artery was similar in the groups. The activity of the antioxidant enzymes in the blood plasma was significant lower in the control group than in the treatment group. The histopathology findings revealed the treatment group to have less damage than the control group. The results of this study suggest that ascorbic acid alone might play a role in attenuating I/R injury and assist in the recovery of the renal function in a renal transplantation model.
Lee
Jae-il
JI
Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea. jaeil@snuh.org
Son
Hwa-Young
HY
Kim
Myung-cheol
MC
D000818:Animals; D001205:Ascorbic Acid / Q000627:therapeutic use*; D001794:Blood Pressure; D001806:Blood Urea Nitrogen; D002374:Catalase / Q000097:blood; D003404:Creatinine / Q000097:blood; D004283:Dog Diseases / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D004285:Dogs / Q000601:surgery*; D005260:Female; D016166:Free Radical Scavengers / Q000627:therapeutic use; D005979:Glutathione Peroxidase / Q000097:blood; D006651:Histocytochemistry / Q000662:veterinary; D016030:Kidney Transplantation / Q000473:pathology / Q000662:veterinary*; D008297:Male; D011897:Random Allocation; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D013482:Superoxide Dismutase / Q000097:blood
D016166:Free Radical Scavengers; D003404:Creatinine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid