title: Association of loss of heterozygosity and PTEN gene abnormalities with paraclinical, clinical modalities and survival time of glioma patients in Malaysia.
abstract: BACKGROUND
  The pattern of allelic loss of heterozygosity (LOH) and PTEN mutations appear to be associated with the progression of gliomas leading to a decrement in the survival rate of patients. This present study was carried out to determine the LOH and PTEN mutational status in glioma patients and its association with patients' survival.
  
  
  METHODS
  Thirty-seven Malaysian glioma patients of the Malay race were subject to PTEN mutational analysis and the presence of LOH using the cold single-strand conformation polymorphism method, and their clinical and paraclinical response were correlated.
  
  
  RESULTS
  Among analysed glioma patients, seven (21.6%) cases with PTEN mutations were detected and 12 (32.4%) of 37 patients showed presence of LOH. Univariate analysis showed that tumour grade, vascularization, PTEN mutation, LOH and combination of both PTEN mutation and LOH were significantly associated with glioma patients' survival. Multivariate analysis revealed that no factors contributed to survival time.
  
  
  CONCLUSIONS
  The results show that PTEN mutation and LOH are quite frequent in Malaysian glioma patients. However, they have no impact on the survival outcome of patients.
authors.affiliation: Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia. deptneurosciencesppspusm@yahoo.com
authors.forename: Jafri Malin
authors.initials: JM
authors.lastname: Abdullah
authors.forename: Ahmad
authors.initials: A
authors.lastname: Farizan
authors.forename: Ku
authors.initials: K
authors.lastname: Asmarina
authors.forename: Norafiza
authors.initials: N
authors.lastname: Zainuddin
authors.forename: Mazira Mohamad
authors.initials: MM
authors.lastname: Ghazali
authors.forename: Hasnan
authors.initials: H
authors.lastname: Jaafar
authors.forename: Mohd Nizam
authors.initials: MN
authors.lastname: Isa
authors.forename: Nyi Nyi
authors.initials: NN
authors.lastname: Naing
mesh_terms: D000328:Adult; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D003627:Data Interpretation, Statistical; D005260:Female; D005910:Glioma / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D006579:Heterozygote; D006801:Humans; D008296:Malaysia; D008297:Male; D015999:Multivariate Analysis; D009154:Mutation; D009367:Neoplasm Staging; D051059:PTEN Phosphohydrolase; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis; D012044:Regression Analysis; D016019:Survival Analysis
chemical_list: D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human