Insulin-like signaling and the neural circuit for integrative behavior in C. elegans.
Caenorhabditis elegans exhibits a food-associated behavior that is modulated by the past cultivation temperature. Mutations in INS-1, the homolog of human insulin, caused the defect in this integrative behavior. Mutations in DAF-2/insulin receptor and AGE-1/phosphatidylinositol 3 (PI-3)-kinase partially suppressed the defect of ins-1 mutants, and a mutation in DAF-16, a forkhead-type transcriptional factor, caused a weak defect. In addition, mutations in the secretory protein HEN-1 showed synergistic effects with INS-1. Expression of AGE-1 in any of the three interneurons, AIY, AIZ, or RIA, rescued the defect characteristic of age-1 mutants. Calcium imaging revealed that starvation induced INS-1-mediated down-regulation of AIZ activity. Our results suggest that INS-1, in cooperation with HEN-1, antagonizes the DAF-2 insulin-like signaling pathway to modulate interneuron activity required for food-associated integrative behavior.
Kodama
Eiji
E
Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Japan.
Kuhara
Atsushi
A
Mohri-Shiomi
Akiko
A
Kimura
Koutarou D
KD
Okumura
Masatoshi
M
Tomioka
Masahiro
M
Iino
Yuichi
Y
Mori
Ikue
I
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D005247:Feeding Behavior; D007328:Insulin / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000502:physiology*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D011972:Receptor, Insulin / Q000037:antagonists & inhibitors* / Q000235:genetics; D015398:Signal Transduction; D013489:Suppression, Genetic; D013696:Temperature
D029742:Caenorhabditis elegans Proteins; C459682:HEN-1 protein, C elegans; D007328:Insulin; D009419:Nerve Tissue Proteins; C424903:ins-1 protein, C elegans; D019869:Phosphatidylinositol 3-Kinases; C100662:AGE-1 protein, C elegans; C107483:DAF-2 protein, C elegans; D011972:Receptor, Insulin