Detection of sentinel nodes by a novel red-fluorescent dye, ATX-S10Na (II), in an orthotopic xenograft rat model of human gastric carcinoma.
OBJECTIVE
We developed a new imaging system to detect sentinel nodes (SNs) using a novel fluorescent tracer, ATX-S10Na(II), and investigated its usefulness in an animal model.


METHODS
Human gastric carcinoma cells were implanted orthotopically into nude rats. ATX-S10Na(II) was injected subserosally into the primary tumor lesion, and visualized by a fluorescence spectro-laparoscope. Presence of tumor cells in lymph nodes (LNs) was determined by RT-PCR specific for human beta-actin.


RESULTS
Injection of ATX-S10Na(II) was successful in 27 tumor-bearing rats. A red fluorescence was incorporated into the left gastric and hepatic LNs in 25 and 2 rats, respectively. Of note, human beta-actin was detected in most of these LNs. Fluorescence was not detected in LNs that did not contain cancer.


CONCLUSIONS
ATX-S10Na(II) is useful for the detection of cancer-containing SNs in an animal model of gastric carcinoma, and may serve as a novel tracer in SN navigation surgery.
Koyama
Tomoki
T
Institute of Clinical Medicine and Research (ICMR), Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan.
Tsubota
Akihito
A
Nariai
Koichi
K
Yoshikawa
Tetsuya
T
Mitsunaga
Makoto
M
Sumi
Makoto
M
Nimura
Hiroshi
H
Yanaga
Katsuhiko
K
Yumoto
Yoko
Y
Mabashi
Yasuo
Y
Takahashi
Hiroshi
H
D000199:Actins / Q000378:metabolism; D000818:Animals; D018395:CA-19-9 Antigen / Q000097:blood; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008207:Lymphatic Metastasis / Q000175:diagnosis*; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D011166:Porphyrins; D051381:Rats; D011916:Rats, Inbred F344; D011923:Rats, Nude; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021701:Sentinel Lymph Node Biopsy; D013050:Spectrometry, Fluorescence; D013274:Stomach Neoplasms / Q000473:pathology*
C117772:ATX S10Na(II); D000199:Actins; D018395:CA-19-9 Antigen; D005456:Fluorescent Dyes; D011166:Porphyrins