[The method of the simultaneous determination of the citrinin and ochratoxin A content in cereals and cereal products].
The simplifyed method of simultaneous determination of the citrinin (CT) and ochratoxin A (OTA) in cereals is described. The extraction of mycotoxins was carried out by small volumes of solvents (water/aceton/hexane) without additional sample clean up and concentration. The extracts were analysed by HPLC using mixture of methanol/ethylacetate/phosphoric acid, pH 2.2 as a mobile phase and fluorescence detection (lamda ex 330 nm, lamda em 495 nm). The reliability and reproducibility of results were improved by usage of internal standard--methyl-derivative of ochratoxin A. Average recovery of CT and OTA was 70%, relative standard deviation 12% and 7%, respectively, limit of detection 0.003 mg/kg.
Eller
K I
KI
Pimenova
V V
VV
Kiseleva
M G
MG
Perederiaev
O I
OI
Aksenov
I V
IV
Medvedev
Iu V
IuV
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002953:Citrinin / Q000032:analysis*; D002523:Edible Grain / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D009793:Ochratoxins; C025589:ochratoxin A; D002953:Citrinin