Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment.
This study defines the in vitro phenomenon of ciliated bovine bronchial epithelial cell (BBEC) detachment from the basal epithelium and regulation of cilia motility mediated through protein kinase C epsilon (PKCepsilon). The authors determined the time course of activation and downregulation of PKCepsilon by the known PKC activator phorbol 12-myristate 13-acetate (PMA) and demonstrate that chemical inhibition of PKC by calphostin C or the novel PKC isoform inhibitor Ro 31-8220 induced striking detachment of ciliated BBECs from the basal cell monolayer within 1 hour, independent of apoptosis or necrotic cell death. The results of this study support a possible novel PKCepsilon-mediated signaling pathway through which ciliated cell attachment is maintained.
Slager
Rebecca E
RE
Pulmonary, Critical Care and Sleep Medicine Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198-5300, USA.
Sisson
Joseph H
JH
Pavlik
Jaqueline A
JA
Johnson
Jennifer K
JK
Nicolarsen
Jeremy R
JR
Jerrells
Thomas R
TR
Wyatt
Todd A
TA
D000818:Animals; D001980:Bronchi / Q000166:cytology*; D002273:Carcinogens / Q000494:pharmacology; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002923:Cilia / Q000187:drug effects / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D009281:Naphthalenes / Q000494:pharmacology; D051744:Protein Kinase C-epsilon / Q000037:antagonists & inhibitors* / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology* / Q000201:enzymology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002273:Carcinogens; D004791:Enzyme Inhibitors; D007211:Indoles; D009281:Naphthalenes; C061465:calphostin complex; D051744:Protein Kinase C-epsilon; D013755:Tetradecanoylphorbol Acetate; C064758:Ro 31-8220