Rapid changes in liver lipid composition and pancreatic islet K+ handling and secretory behaviour provoked by the intravenous administration of a medium-chain triglyceride: fish oil emulsion to long-chain polyunsaturated omega3 fatty acid-depleted rats.
Second generation rats depleted in long-chain polyunsaturated omega3 fatty acids are currently used as an animal model for the insufficient dietary supply of such fatty acids often prevailing in Western populations. The present study deals mainly with the effects of a novel medium-chain triglyceride: fish oil emulsion (MCT:FO), as compared to a control medium-chain triglyceride:olive oil emulsion (MCT: OO), administered as an intravenous bolus to the omega3-depleted rats 60-120 min before sacrifice upon selected biochemical and biophysical variables. The major findings consisted of a severe decrease of the omega3 fatty acid content of liver lipids in non-injected omega3-depleted rats and its partial correction after injection of the MCT:FO emulsion. The omega3-depleted rats also displayed liver steatosis, increased incorporation of long-chain polyunsaturated omega6 fatty acids in liver phospholipids and increased activity of liver Delta9-desaturase. As judged from the effects of ouabain upon 86Rb net uptake by isolated pancreatic islets, the activity of Na+,K+-ATPase was virtually abolished in the omega3-depleted rats. The latter defect was corrected by prior intravenous injection of the MCT:FO emulsion, this coinciding with suppression of the excessive secretory response to a number of insulin secretagogues otherwise observed in the islets of omega3-depleted rats injected or not with the MCT:OO emulsion.
Louchami
Karim
K
Laboratory of Experimental Hormonology, Brussels Free University, B-1070 Brussels, Belgium.
Zhang
Ying
Y
Oguzhan
Berrin
B
Delporte
Christine
C
Portois
Laurence
L
Carpentier
Yvon A
YA
Genten
Franck
F
Danguy
Andre
A
Malaisse
Willy J
WJ
Sener
A
A
D000818:Animals; D002412:Cations / Q000378:metabolism*; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood / Q000737:chemistry; D015525:Fatty Acids, Omega-3 / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis / Q000737:chemistry; D005260:Female; D005395:Fish Oils / Q000737:chemistry; D007515:Islets of Langerhans / Q000378:metabolism / Q000502:physiology*; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D014280:Triglycerides / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry* / Q000494:pharmacology*
D002412:Cations; D005217:Fat Emulsions, Intravenous; D005230:Fatty Acids, Nonesterified; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D014280:Triglycerides