Halorubrum orientale sp. nov., a halophilic archaeon isolated from Lake Ejinor, Inner Mongolia, China.
A motile, pleomorphic, red-pigmented archaeon, strain EJ-52T, was isolated from water from Lake Ejinor, a saline lake in Inner Mongolia, China. Analysis of the almost-complete 16S rRNA gene sequence showed that the isolate was phylogenetically related to species of the genus Halorubrum, being most closely related to Halorubrum saccharovorum ATCC 29252T (96.1% sequence similarity), Halorubrum lacusprofundi JCM 8891T (95.9%), Halorubrum tibetense AS 1.3239T (95.2%), Halorubrum alcaliphilum AS 1.3528T (95.2%) and Halorubrum vacuolatum JCM 9060T (95.1%). The polar lipids of strain EJ-52T were C20C20 derivatives of phosphatidylglycerol phosphate and phosphatidylglycerol phosphate methyl ester and a sulfated diglycosyl diether. Strain EJ-52T requires at least 2.5 M NaCl for growth and grows optimally at 3.4 M NaCl. The strain grows at 25-50 degrees C, with optimal growth occurring at 35-45 degrees C. Mg2+ is not required. The DNA G+C content is 64.2 mol%. On the basis of the data obtained in this study, strain EJ52T represents a novel species, for which the name Halorubrum orientale sp. nov. is proposed. The type strain is EJ-52T (=CECT 7145T=JCM 13889T=CGMCC 1.6295T).
Castillo
A M
AM
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Gutiérrez
M C
MC
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Kamekura
M
M
Noda Institute for Scientific Research, 399 Noda, Noda-shi, Chiba-ken 278-0037, Japan.
Xue
Y
Y
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China.
Ma
Y
Y
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China.
Cowan
D A
DA
Department of Biotechnology, University of the Western Cape, Bellville 7535, Cape Town, South Africa.
Jones
B E
BE
Genencor International BV, Archimedesweg 30, 2333 CN Leiden, The Netherlands.
Grant
W D
WD
Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, UK.
Ventosa
A
A
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
D001482:Base Composition; D002681:China; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D006216:Halobacteriaceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D008274:Magnesium / Q000378:metabolism; D008969:Molecular Sequence Data; D009068:Movement; D010802:Phylogeny; D010860:Pigments, Biological / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000032:analysis / Q000378:metabolism; D013696:Temperature; D014871:Water Microbiology
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D008055:Lipids; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D008274:Magnesium