[Influence of meliorated AS-PCR primer for ABO genotyping].
OBJECTIVE
To improve the correct rate of ABO genotyping by meliorating AS-PCR primer.


METHODS
The primer P1 was changed into primer P1' by substituting the fifth base G for C of 3' end and the ABO genotyping results of primer P1 and P1' was compared and analysed.


RESULTS
The non-specific product of OO typing is reducing and the wrong genotyping of OO and AO was avoided by meliorating AS-PCR primer.


CONCLUSIONS
The rates of wrong ABO genotyping results could be effectively reduced by using altering primer P1'.
Wang
Xin-Jie
XJ
Weifang Public Security Bureau, Weifang 261061, China.
Diao
Li-Jiang
LJ
Feng
Jian-Zhong
JZ
Lu
Gui-Ping
GP
D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D000017:ABO Blood-Group System; D017931:DNA Primers