Polyribosomes are increased in spines of CA1 dendrites 2 h after the induction of LTP in mature rat hippocampal slices.
Enduring long-term potentiation (LTP) requires immediate protein synthesis, hence we assessed whether more polyribosomes are present in dendritic spines of mature hippocampal dendrites after the induction of LTP. Reconstructions from serial section transmission electron microscopy (sSTEM) revealed more dendritic polyribosomes 2 h posttetanus, relative to low-frequency stimulation (LFS). Polyribosomes were present in spines of all shapes with larger postsynaptic densities after 2 h, suggesting a coordinated local protein synthesis among many synapses to replenish proteins utilized during an earlier phase of LTP.
Bourne
Jennifer N
JN
Synapses and Cognitive Neuroscience Center, Medical College of Georgia, Augusta, Georgia, USA.
Sorra
Karin E
KE
Hurlburt
Jamie
J
Harris
Kristen M
KM
D000818:Animals; D049229:Dendritic Spines / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D004558:Electric Stimulation / Q000379:methods; D006624:Hippocampus / Q000166:cytology; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000502:physiology*; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008953:Models, Anatomic; D009474:Neurons / Q000528:radiation effects / Q000648:ultrastructure; D011132:Polyribosomes / Q000378:metabolism*; D051381:Rats; D020318:Rats, Long-Evans; D013997:Time Factors