Milestones in electron crystallography.
Electron crystallography determines the structure of membrane embedded proteins in the two-dimensionally crystallized state by cryo-transmission electron microscopy imaging and computer structure reconstruction. Milestones on the path to the structure are high-level expression, purification of functional protein, reconstitution into two-dimensional lipid membrane crystals, high-resolution imaging, and structure determination by computer image processing. Here we review the current state of these methods. We also created an Internet information exchange platform for electron crystallography, where guidelines for imaging and data processing method are maintained. The server (http://2dx.org) provides the electron crystallography community with a central information exchange platform, which is structured in blog and Wiki form, allowing visitors to add comments or discussions. It currently offers a detailed step-by-step introduction to image processing with the MRC software program. The server is also a repository for the 2dx software package, a user-friendly image processing system for 2D membrane protein crystals.
Renault
Ludovic
L
Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave., Davis, CA 95616, USA.
Chou
Hui-Ting
HT
Chiu
Po-Lin
PL
Hill
Rena M
RM
Zeng
Xiangyan
X
Gipson
Bryant
B
Zhang
Zi Yan
ZY
Cheng
Anchi
A
Unger
Vinzenz
V
Stahlberg
Henning
H
D020285:Cryoelectron Microscopy / Q000379:methods* / Q000639:trends*; D018360:Crystallography, X-Ray / Q000639:trends; D020407:Internet; D008565:Membrane Proteins / Q000737:chemistry; D014584:User-Computer Interface
D008565:Membrane Proteins