Cell turnover and activity in mammary tissue during lactation and the dry period in dairy cows.
Milk yield of the dairy cow follows a pattern termed the lactation curve. We have investigated the cellular background for this pattern. Seven mammary biopsies were obtained from each of 10 cows: at the end of lactation (d 347, equal to d 77 before next parturition); during the dry period at d 48 (4 d after dry off); 16 d before parturition; and during lactation at d 14, 42, 88, and 172. The fraction of proliferating (staining positive for Ki-67) alveolar cells was higher during the dry period (8.6%) than during lactation (0.5%). The fraction of apoptotic (staining positive by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) alveolar cells was higher immediately after dry off (0.37%) and in early lactation (0.76%) than during other periods (0.15%). The enzyme activities of fatty acid synthetase, acetyl CoA-carboxylase, and galactosyl transferase were approximately 12-, 11-, and 4-fold higher, respectively, during lactation than during the dry period. In conclusion, mammary cell proliferation is substantial in a period near parturition but otherwise low, and apoptosis is elevated at dry off and in early lactation. The increase in apoptosis in early lactation may be due to discarding nonfunctional or senescent cells or to removal of a surplus of newly synthesized cells. The activity of selected enzymes central for milk synthesis is probably not limiting for milk production.
Sorensen
M T
MT
Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830, Tjele, Denmark. MartinT.Sorensen@agrsci.dk
Nørgaard
J V
JV
Theil
P K
PK
Vestergaard
M
M
Sejrsen
K
K
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001707:Biopsy, Needle / Q000662:veterinary; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002417:Cattle / Q000502:physiology*; D049109:Cell Proliferation; D003612:Dairying; D005260:Female; D015870:Gene Expression / Q000502:physiology; D006728:Hormones / Q000096:biosynthesis / Q000097:blood; D020287:In Situ Nick-End Labeling / Q000662:veterinary; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000235:genetics / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D007774:Lactation / Q000502:physiology*; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000201:enzymology / Q000502:physiology*; D049590:Postpartum Period / Q000502:physiology; D011247:Pregnancy; D013997:Time Factors
D006728:Hormones; D018975:Insulin-Like Growth Factor Binding Protein 5; D007334:Insulin-Like Growth Factor I; D053148:Caspase 3