title: [Significance of hepatitis B virus PreS1-Ag, PreS2-Ag, large protein, PreS2-Ab detection and the prediction of HBV DNA replication.].
abstract: BACKGROUND
  To explore the significance of hepatitis B virus PreS1-Ag, PreS2-Ag, large protein (LP) detection and the prediction of viral replication.
  
  
  METHODS
  PreS1-Ag, PreS2-Ag, LP and HBV markers were measured by enzyme linked immunosorbent assay (ELISA) in 201 cases of infected serum. Serum HBV DNA level was quantitatively detected by real-time polymerase chain reaction (PCR).
  
  
  RESULTS
  There were significant differences in positive rate between the PreS1-Ag, PreS2-Ag, LP, and HBsAg; the positive rate of PreS2-Ag and LP were higher than that of the HBeAg. No significant differences were found in the positive rates between LP and the levels of HBV DNA and there was a positive correlation between quantitations of HBV DNA and HBV-LP.
  
  
  CONCLUSIONS
  Serum PreS1-Ag, PreS2-Ag and LP were laboratory markers that can accurately reflect HBV DNA reproduction, and were helpful complementarity to traditional HBV M. There is a close correlation between the number of copies of HBV DNA and the levels of HBV-LP.
authors.affiliation: Center of Clinical Laboratory, The No. 302 Hospital of The People's Liberation Army, Beijing 100039, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Mao
authors.forename: B A
authors.initials: BA
authors.lastname: Li
authors.forename: H B
authors.initials: HB
authors.lastname: Ma
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Sun
authors.forename: J B
authors.initials: JB
authors.lastname: Shi
authors.forename: X H
authors.initials: XH
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: X F
authors.initials: XF
authors.lastname: Wang
authors.forename: L H
authors.initials: LH
authors.lastname: Yang
mesh_terms: D004261:DNA Replication; D004279:DNA, Viral / Q000097:blood; D006509:Hepatitis B / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D011498:Protein Precursors / Q000097:blood
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors