[Purification and activity calculated of fibrinolyric factors from Eupolyphaga sinensis].
Three kinds ( EFF-1, EFF-2 and EFF-3) of fibrinolytic factor were separated by ammonium sulphate precipitation, DEAE-cellulose and preparative PAGE electrophoresis from female Eupolyphaga sinensis Walker. Their molecular weights were proved to be 41kd, 32.9 kd and 30.6 kd respectively with SDS-PAGE electophoresis. Their Activities as plasminogen activator were 171.3 U/mg, 234.0 U/mg and 148.5 U/mg. In addition, EFF-2 and EFF-3 were not only fibrinolytic activities but also have plasminogen activator on fibrinous plate lacked of plasminogen . There had been no such components of plasminogen activator and fiberinolytic enzyme from Eupolyphaga sinensis reported yet.
Han
Ya-Li
YL
Bioengineering Department, Guangdong University of Technology, Guangzhou 510090, China.
Li
Zhang-Wei
ZW
D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification; D007313:Insecta / Q000737:chemistry*; D008419:Materia Medica / Q000737:chemistry / Q000302:isolation & purification*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D010960:Plasminogen Activators / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; C470734:EFF-1 protein, C elegans; D005343:Fibrinolytic Agents; D008419:Materia Medica; D008562:Membrane Glycoproteins; D010960:Plasminogen Activators; D005341:Fibrinolysin