Expression, purification, crystallization and preliminary X-ray diffraction analysis of alpha-11 giardin from Giardia lamblia.
Alpha-11 Giardin, a protein from the annexin superfamily, is a 35.0 kDa protein from the intestinal protozoan parasite Giardia lamblia which triggers a form of diarrhea called giardiasis. Here, the cloning, expression, purification and the crystallization of alpha-11 giardin under two different conditions and in two different space groups is reported. Crystals from the first condition diffracted to 1.1 A and belong to a primitive orthorhombic space group, while crystals from the second condition, which included calcium in the crystallization solution, diffracted to 2.93 A and belong to a primitive monoclinic space group. Determination of the detailed atomic structure of alpha-11 giardin will provide a better insight into its biological function and might establish whether this class of proteins is a potential drug target against giardiasis.
Pathuri
Puja
P
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA.
Nguyen
Emily Tam
ET
Luecke
Hartmut
H
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D016829:Giardia lamblia; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D016415:Sequence Alignment; D014961:X-Ray Diffraction
D003598:Cytoskeletal Proteins; D017931:DNA Primers; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C038207:giardin protein, Giardia lamblia