title: [Effects of Pollen Typhae total flavone on glucose and lipid metabolism in 3T3-L1 adipocytes].
abstract: OBJECTIVE
  To observe the effects of Pollen Typhae total flavone (PTF) on glucose and lipid metabolism in 3T3-L1 adipocytes.
  
  
  METHODS
  The content of glucose which disappeared from the culture medium after incubation with drugs for 24 hours was determined as glucose consumption of the cells. The activity of cells was detected by XTT method. The transport of glucose was observed by (3)H-glucose uptake method. The efflux of free fatty acid (FFA) from adipocytes was observed by the concentration of FFA in the culture medium.
  
  
  RESULTS
  The glucose concentration in culture medium was significantly decreased with a concentration-dependent effect, when PTF concentrations were from 0.025 g/L to 0.4 g/L. The toxic effect on cells appeared while PTF concentration was 0.4 g/L, and the MTT value decreased. PTF also significantly increased glucose transportation in the 3T3-L1 adipocytes as rosiglitazone (ROS) did. At the same time, FFA concentration in culture medium was significantly decreased as compared to the normal control group, while ROS-treated group did not show any difference.
  
  
  CONCLUSIONS
  PTF can increase insulin sensitivity by increasing glucose transportation and consumption in the 3T3-L1 adipocytes as well as decreasing the FFA efflux from the cells.
authors.affiliation: Institute of Chinese Integrative Medicine, Fudan University, Shanghai 200040, China.
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: He
authors.forename: Wen-Jian
authors.initials: WJ
authors.lastname: Wang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Ying
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: He
authors.forename: Yu-Yin
authors.initials: YY
authors.lastname: Ma
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: You-Shen
authors.initials: YS
authors.lastname: Li
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D047309:Flavones; D005419:Flavonoids / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D051379:Mice; D011058:Pollen / Q000737:chemistry; D032401:Typhaceae / Q000737:chemistry*
chemical_list: D005230:Fatty Acids, Nonesterified; D047309:Flavones; D005419:Flavonoids; D005947:Glucose; C043562:flavone