title: [Impact of mobilization with rhG-CSF on the proliferation and cytotoxicity of donor's T cells].
abstract: The study was to understand the impact on the proliferation and cytotoxicity of donor's T cells during mobilization with rhG-CSF. The peripheral blood mononuclear cells (PBMNC) were collected from 15 donors before mobilization and on fifth day of mobilization with rhG-CSF. After the PBMNC were activated with 500 ng/ml of CD3 monoclonal antibody and 500 microg/ml of rhIL-2 for 96 hours, the activated T cells were collected for testing proliferation, cytotoxicity, Fas expression, perforin and Fas ligand (FasL) mRNA expression, the IFN-gamma concentration in the culture medium of the activated T cells was determined by radioimmunoassay. The results showed that the proliferation activity of T lymphocytes and the cytotoxicity of T cells activated with CD3 monoclonal antibody and rhIL-2 were reduced markedly after mobilization with rhG-CSF (P < 0.05). The Fas molecule expression in the activated T cells was very high both before and after mobilization with rhG-CSF (P > 0.10). The activated T cells expressed perforin mRNA and didn't express FasL mRNA both before and after mobilization with rhG-CSF. The concentration of IFN-gamma in the culture medium of the activated T cells decreased significantly after mobilization with rhG-CSF (P < 0.01). It is concluded that activity of proliferation and cytotoxicity of donor's T cells is impaired after mobilization with rhG-CSF.
authors.affiliation: Department of Hematology, General Hospital of PLA, Beijing 100853, China.
authors.forename: Wen-Rong
authors.initials: WR
authors.lastname: Huang
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Wang
authors.forename: Chun-Ji
authors.initials: CJ
authors.lastname: Gao
authors.forename: Zhuo-Zhuang
authors.initials: ZZ
authors.lastname: Lu
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Duan
authors.forename: Wan-Ming
authors.initials: WM
authors.lastname: Da
mesh_terms: D000293:Adolescent; D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D053222:Fas Ligand Protein / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology; D019014:fas Receptor / Q000096:biosynthesis / Q000235:genetics
chemical_list: D053222:Fas Ligand Protein; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019014:fas Receptor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor