Differential uptake of photosynthetic and non-photosynthetic proteins by pea root plastids.
The photosynthetic proteins RuBiSCO, ferredoxin I and ferredoxin NADP(+)-oxidoreductase (pFNR) were efficiently imported into isolated pea chloroplasts but not into pea root plastids. By contrast non-photosynthetic ferredoxin III and heterotrophic FNR (hFNR) were efficiently imported into both isolated chloroplasts and root plastids. Chimeric ferredoxin I/III (transit peptide of ferredoxin I attached to the mature region of ferredoxin III) only imported into chloroplasts. Ferredoxin III/I (transit peptide of ferredoxin III attached to the mature region of ferredoxin I) imported into both chloroplasts and root plastids. This suggests that import depends on specific interactions between the transit peptide and the translocon apparatus.
Yan
Xianxi
X
Faculty of Life Sciences, The University of Manchester, 3.614 Stopford Building, Oxford Road, Manchester, M13 9PT, UK.
Khan
Sultan
S
Hase
Toshiharu
T
Emes
Michael J
MJ
Bowsher
Caroline G
CG
D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism; D018532:Pisum sativum / Q000235:genetics / Q000378:metabolism*; D010788:Photosynthesis / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D018087:Plastids / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000235:genetics
D010940:Plant Proteins