Bionanoconjugation via click chemistry: The creation of functional hybrids of lipases and gold nanoparticles.
A simple and versatile method for the preparation of functional enzyme-gold nanoparticle conjugates using "click" chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalized Thermomyces lanuginosus lipase has been attached to azide-functionalized water-soluble gold nanoparticles under retention of enzymatic activity. The products have been characterized by gel electrophoresis and a fluorometric lipase activity assay. It is estimated that the equivalent of approximately seven fully active lipase molecules are attached to each nanoparticle.
Brennan
Jennifer L
JL
Centre for Nanoscale Science, Department of Chemistry, University of Liverpool, Liverpool, L69 7ZD, U.K.
Hatzakis
Nikos S
NS
Tshikhudo
T Robert
TR
Dirvianskyte
Nijole
N
Razumas
Valdemaras
V
Patkar
Shamkant
S
Vind
Jesper
J
Svendsen
Allan
A
Nolte
Roeland J M
RJ
Rowan
Alan E
AE
Brust
Mathias
M
D006046:Gold / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*
D006046:Gold; D008049:Lipase