title: [Study of dicyclohexyl phthalate on preparation and characterization of artificial antigen].
abstract: OBJECTIVE
  To study the preparation and characterization of hapten and artificial antigen of dicyclohexyl phthalate.
  
  
  METHODS
  A dicyclohexyl phthalate hapten (4-amino dicyclohexyl phthalate) was synthesized by introducing amino as a substituent on the aromatic ring and retaining the ester group, and characterized by 1HNMR, IR and UV. The hapten was conjugated to BSA via amino diazotization linkage.
  
  
  RESULTS
  Lambda1 = 214nm, lambda2 = 256nm for the UV of dicyclohexyl 4-nitrophthalate and lambda1 = 226nm, lambda2 = 288nm for the UV of dicyclohexyl 4-aminophthalate. Artificial antigen was prepared and tested by fluorescence, and lambda(ex) = 307nm, lambda(em) = 468nm, and the approximate molar ratio of dicyclohexyl 4-aminophthalate to BSA was 19. The product was used as an immunogen, demonstrating that it is suitable for polyclonal antibody production.
  
  
  CONCLUSIONS
  It is a good method for preparation of artificial antigen of dicyclohexyl phthalate by introducing amino as a substituent on the aromatic ring and retaining the ester group. It was suggested that could supply excellent immune antigen for further preparation of antibody and immunoassay to dicyclohexyl phthalate.
authors.affiliation: College of Environmental Science and Engineering, Donghua University, Shanghai 200051, China.
authors.forename: Ming-Cui
authors.initials: MC
authors.lastname: Zhang
authors.forename: Hui-Sheng
authors.initials: HS
authors.lastname: Zhuang
authors.forename: Qing
authors.initials: Q
authors.lastname: Lang
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D008297:Male; D010795:Phthalic Acids / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits
chemical_list: D000941:Antigens; D010795:Phthalic Acids; C036042:dicyclohexyl phthalate