[Changes of cyclin D1 expression following human brain contusion].
OBJECTIVE
To study the relationships of Cyclin D1 expression with the posttraumatic intervals (PTI) following the cerebra, brainstem or cerebella contusion in human.


METHODS
88 cases of brain contusions of the closed head injury were investigated with pathological and Cyclin D1 immunohistochemistry methods. The results were analyzed by image analysis technique (IAT).


RESULTS
The immunoreactivity of Cyclin D1 was almost disappeared in the core cells of the brain contusion. Cyclin D1-positive cells started to increase in the boundary of the brain contusion in the 1h group. Cyclin D1-positive cells were increased significantly in the 3 h-30 d groups and maintained at a high level in the boundary of the brain contusion of those groups. It is suggested that the Cyclin D1-positive cells were primarily origin from microglia and other glia. A few neurons expressed Cyclin D1.


CONCLUSIONS
Cyclin D1 can express in several kinds of brain cells following the contusion, especially in the glia cells. Cyclin D1-positive cells were increased obviously and rapidly after injury, so it could be used as a reference marker for early stage brain injury.
Zhou
Yi-Wu
YW
Department of Forensic Medicine, Shantou Medical College, Shantou University, Shantou 515031, China. yiwuhedi@hotmail.com
Xiao
Ai-Wu
AW
Deng
Wei-Nian
WN
Sun
Lai-Jing
LJ
Shu
Xi-Ji
XJ
Dai
Jia-Pei
JP
Liu
Liang
L
Xu
Xiao-Hu
XH
D000293:Adolescent; D000328:Adult; D000368:Aged; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D019938:Cyclin D1 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009457:Neuroglia / Q000378:metabolism*; D013194:Staining and Labeling; D013997:Time Factors; D055815:Young Adult
C530104:CCND1 protein, human; D019938:Cyclin D1