Antiplasmodial activity of ineupatorolides A from Carpesium rosulatum.
Samples of Carpesium genus used as traditional remedies for the treatment of parasite infections were collected, and methanol extracts were obtained by sonication. The ethylacetate-, n-butanol- and H(2)O-soluble fractions exhibited weak antiplasmodial activity (IC(50) > 100 microg/ml; IC(50), 50% inhibitory concentration). However, the chloroform fraction exhibited more impressive antiplasmodial activity (IC(50) = 8.2 microg/ml). The antiplasmodial activity of the chloroform fractions was evaluated in vitro against the chloroquine-resistant D10 strain of Plasmodium falciparum. Bioactivity-guided isolation of the chloroform fractions of the whole plants of Carpesium rosulatum has led to the isolation of a sesquiterpene lactone, ineupatorolides A, displaying high antiplasmodial activity (IC(50) = 0.007 microg/ml). This is the first report of the isolation of ineupatorolides A from this species and of its remarkable antiplasmodial activity.
Moon
Hyung-In
HI
National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, Oxford, MS 38677, USA. himoon@olemiss.edu
D000818:Animals; D000962:Antimalarials / Q000302:isolation & purification* / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000187:drug effects*; D012717:Sesquiterpenes / Q000302:isolation & purification* / Q000494:pharmacology*
D000962:Antimalarials; D010936:Plant Extracts; D012717:Sesquiterpenes; C524000:ineupatorolides A