Leishmania (Kinetoplastida): species typing with isoenzyme and PCR-RFLP from cutaneous leishmaniasis patients in Ethiopia.
Cutaneous leishmaniasis (CL) is an increasing public health problem in Ethiopia. There is a concern that it is spreading with increased incidence. In this study, we used isoenzyme electrophoresis and internal transcribed spacer one (ITS1) PCR-RFLP techniques to identify Leishmania species from CL patients in Ethiopia. We obtained isolates from 55 localized cutaneous leishmaniasis (LCL), 3 diffused cutaneous leishmaniasis (DCL) and 36 biopsy samples from 34 LCL and 2 DCL cases from All Africa Leprosy and Tuberculosis Rehabilitation and Training Center (ALERT) and clinically diagnosed CL cases from Ochollo village. Both isoenzyme and ITS1 PCR-RFLP techniques showed that Leishmania aethiopica (L. aethiopica) was the aetiologic agent in all cases. Our study also showed that ITS1 PCR-RFLP could identify Leishmania species from biopsy samples and suggests the method could be used for epidemiological surveillance of leishmaniasis in Ethiopia and for species-specific diagnosis.
Gadisa
E
E
Armauer Hansen Research Institute, Addis Ababa, Ethiopia. endalamawgadisa@yahoo.com
Genetu
A
A
Kuru
T
T
Jirata
D
D
Dagne
K
K
Aseffa
A
A
Gedamu
L
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002648:Child; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000032:analysis*; D005002:Ethiopia; D005260:Female; D006801:Humans; D007223:Infant; D007527:Isoenzymes / Q000032:analysis*; D007891:Leishmania / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D016054:DNA, Protozoan; D007527:Isoenzymes