Development and application of RAPD-SCAR marker for identification of Phyllanthus emblica LINN.
Correct genotype identification of medicinal plant material remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. The present study was carried out to develop DNA based marker for identification of Phyllanthus emblica LINN. A putative marker (1.1 kb) specific for P. emblica was identified by Random Amplified Polymorphic DNA (RAPD) technique. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon. The SCAR marker was found useful for identification of P. emblica in its commercial samples and Triphalachurna, a multi-component Ayurvedic formulation.
Dnyaneshwar
Warude
W
Bioprospecting Laboratory, Interdisciplinary School of Health Sciences, University of Pune, India.
Preeti
Chavan
C
Kalpana
Joshi
J
Bhushan
Patwardhan
P
D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D029803:Phyllanthus / Q000145:classification / Q000235:genetics; D029842:Phyllanthus emblica / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D018744:DNA, Plant; D005819:Genetic Markers