Vitamin K1 accumulation in tobacco plants overexpressing bacterial genes involved in the biosynthesis of salicylic acid.
Phylloquinone (Vitamin K(1)) is an essential component of the photosynthetic electron transfer. As isochorismate is required for the biosynthesis of Vitamin K(1), isochorismate synthase (ICS) activity is expected to be present in all green plants. In bacteria salicylic acid (SA) is synthesized via a two step pathway involving ICS and isochorismate pyruvate lyase (IPL). The effect of the introduction in tobacco plants of the bacterial ICS and IPL genes on the endogenous isochorismate pathway was investigated. Transgenic tobacco plants in which IPL was targeted to the chloroplast suffered severe growth retardation and had low Vitamin K(1) content. Probably because isochorismate was channeled towards SA production, the plants were no longer able to produce normal levels of Vitamin K(1). Transgenic tobacco plants in which the bacterial ICS was present in the chloroplast showed higher Vitamin K(1) contents than wild type plants.
Verberne
Marianne C
MC
Institute of Biology Leiden, Section Plant Metabolomics, Gorlaeus Laboratories, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands.
Sansuk
Kamonchanok
K
Bol
John F
JF
Linthorst
Huub J M
HJ
Verpoorte
Robert
R
D001426:Bacterial Proteins / Q000235:genetics; D053898:Biosynthetic Pathways / Q000235:genetics*; D019757:Carbon-Oxygen Lyases / Q000235:genetics* / Q000378:metabolism; D002827:Chorismic Acid / Q000378:metabolism; D003509:Cyclohexanecarboxylic Acids / Q000378:metabolism; D053138:Cyclohexenes / Q000378:metabolism; D019751:Intramolecular Transferases / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics; D020156:Salicylic Acid / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D010837:Vitamin K 1 / Q000302:isolation & purification / Q000378:metabolism*
D001426:Bacterial Proteins; D003509:Cyclohexanecarboxylic Acids; D053138:Cyclohexenes; D011994:Recombinant Proteins; D010837:Vitamin K 1; D019757:Carbon-Oxygen Lyases; C517864:isochorismate pyruvate lyase, Nicotiana tabacum; D019751:Intramolecular Transferases; C058486:isochorismate synthase; D002827:Chorismic Acid; D020156:Salicylic Acid; C005550:prephenic acid