title: Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: a critical review.
abstract: Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1 variant. The literature on DNA extraction methods, primers, PCR strategies, and confirmatory procedures used for the detection and identification of herpesviruses that infect bivalve molluscs are reviewed.
authors.affiliation: Instituto Nacional de Investigação Agrária e das Pescas (INIAP/IPIMAR), CRIPSul, Av. 5 de Outubro, 8700-305 Olhão, Portugal.
authors.forename: Frederico M
authors.initials: FM
authors.lastname: Batista
authors.forename: Isabelle
authors.initials: I
authors.lastname: Arzul
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Pepin
authors.forename: Francisco
authors.initials: F
authors.lastname: Ruano
authors.forename: Carolyn S
authors.initials: CS
authors.lastname: Friedman
authors.forename: Pierre
authors.initials: P
authors.lastname: Boudry
authors.forename: Tristan
authors.initials: T
authors.lastname: Renault
mesh_terms: D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D006564:Herpesviridae / Q000235:genetics*; D008969:Molecular Sequence Data; D010125:Ostreidae / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral