title: [Combined expression of TSO45W-4BX from Taenia solium and porcine CD58 in Escherichia coli].
abstract: OBJECTIVE
  To express the TSO45W-4BX of Taenia solium in combination with CD58 as a molecular adjuvant for improving the protective efficacy of the TSO45W-4BX recombinant vaccine.
  
  
  METHODS
  TSO45W-4BX and porcine CD58 genes were amplified by PCR, using recombinant plasmids pGEM-4B and pGEM-CD58 as template respectively. The CD58 fragment was inserted into the recombinant plasmid pGEX-4T-1 with directly ligated TSO45W-4BX. The transformant was induced with IPTG and followed by identifying the integrity of the recombinant containing TS045W-4BX and porcine CD58 with PCR and sequencing. The products were analyzed by SDS-PAGE and Western blotting.
  
  
  RESULTS
  The expression products of Mr 69,000 GST-4BX/CD58 and Mr 41,000 GST-4BX were present mainly in the form of inclusion bodies and soluble substance respectively, and both were recognized by sera of cysticercosis patients.
  
  
  CONCLUSIONS
  The TSO45W-4BX co-expressed with porcine CD58 conserves its immune reactivity.
authors.affiliation: Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, CAAS, Lanzhou, China.
authors.forename: Xue-nong
authors.initials: XN
authors.lastname: Luo
authors.forename: Ya-dong
authors.initials: YD
authors.lastname: Zheng
authors.forename: Yong-xi
authors.initials: YX
authors.lastname: Dou
authors.forename: Jun-lin
authors.initials: JL
authors.lastname: Hou
authors.forename: Zhi-zhong
authors.initials: ZZ
authors.lastname: Jing
authors.forename: Xue-peng
authors.initials: XP
authors.lastname: Cai
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis* / Q000235:genetics; D018968:CD58 Antigens / Q000096:biosynthesis*; D003551:Cysticercosis / Q000097:blood* / Q000469:parasitology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010957:Plasmids; D016133:Polymerase Chain Reaction; D013552:Swine; D041201:Taenia solium / Q000235:genetics / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D018968:CD58 Antigens