title: [Combined use of transmyocardial stents with gene therapy in the treatment of acute myocardial infarction].
abstract: OBJECTIVE
  To determine the efficacy of combined use of transmyocardial stent with gene therapy to treat acute myocardial infarction in porcine model.
  
  
  METHODS
  24 Chinese mini swines have been divided into 4 groups randomly: group myocardial infarction (group MI n(1) = 6), group transmyocardial stent (group ST n(2) = 6), group vascular endothelial growth factor (group VEGF n(3) = 6), group transmyocardial stent and VEGF (group ST + VEGF n(4) = 6). In group MI, acute myocardial infarction animal model has been established by the ligation of the left descending coronary artery. In group ST, after the establishment of the model, 3 transmyocardial stents were implanted. In group VEGF, an expression plasmid containing the gene-encoding VEGF(165) (300 microg) was administered directly in the myocardium at 6 sites. In group ST + VEGF, both transmyocardial stents and expression plasmid containing the gene-encoding VEGF(165) are applied. 4 weeks later, the animals are sacrificed and echocardiography and pathological analysis have been done.
  
  
  RESULTS
  The density of blood vessel in group ST, VEGF and ST + VEGF are significantly higher than group MI. And capillary density in group ST + VEGF is the highest in these groups statistically. Expression of VEGF was detected in group ST, VEGF and ST + VEGF, but in group VEGF and ST + VEGF the level of expression are higher.
  
  
  CONCLUSIONS
  Combined use of transmyocardial stent with gene therapy has synthetic effect for the treatment of acute myocardial ischemia in porcine model and can significantly increase the vascular density.
authors.affiliation: Department of Cardio-thoracic Surgery, Tongji Hospital of Tongji University, Shanghai 200065, China.
authors.forename: Yong-wu
authors.initials: YW
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.forename: Min-lei
authors.initials: ML
authors.lastname: Wang
authors.forename: Yang-yang
authors.initials: YY
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yong-xin
authors.initials: YX
authors.lastname: Zhou
authors.forename: Jian-zhi
authors.initials: JZ
authors.lastname: Cai
authors.forename: Jie
authors.initials: J
authors.lastname: Shao
mesh_terms: D000818:Animals; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D015316:Genetic Therapy; D009203:Myocardial Infarction / Q000628:therapy*; D011897:Random Allocation; D015607:Stents; D013552:Swine; D013556:Swine, Miniature; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D042461:Vascular Endothelial Growth Factor A