title: Immobilization of bovine serum albumin as a sensitive biosensor for the detection of trace lead ion in solution by piezoelectric quartz crystal impedance.
abstract: A biosensor based on bovine serum albumin (BSA) for the detection of lead (Pb(2+)) ion was developed and characterized. BSA was immobilized onto a colloidal Au-modified piezoelectric quartz crystal (PQC) as a biosensor for the detection of Pb(2+) ion by piezoelectric quartz crystal impedance (PQCI). Calibration curves for the quantification of Pb(2+) ion showed excellent linearity throughout the concentration range from 1.0 x 10(-7) to 3.0 x 10(-9)mol/L. The interaction between the Pb(2+) ions and the sensor chip is influenced significantly by the pH of the reaction buffer, and the optimal pH for the experiment was 5.4. Under the optimal conditions, the detection limit of 1.0 x 10(-9)mol/L for Pb(2+) was obtained. Kinetic parameters of the Pb(2+)-BSA interactions were also determined by using this chip. The sensor chip could be regenerated for use by dipping in the ethylenediaminetetraacetic acid (EDTA) solution for approximately 2h, and the chip was used to detect Pb(2+) ion for eight times without obvious signal attenuation.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, People's Republic of China. yinjian1975@hotmail.com
authors.forename: Jian
authors.initials: J
authors.lastname: Yin
authors.forename: Wanzhi
authors.initials: W
authors.lastname: Wei
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Kong
authors.forename: Ling
authors.initials: L
authors.lastname: Wu
authors.forename: Shuguo
authors.initials: S
authors.lastname: Gong
mesh_terms: D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000032:analysis*; D011791:Quartz; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012996:Solutions
chemical_list: D012996:Solutions; D011791:Quartz; D012710:Serum Albumin, Bovine; D007854:Lead; D006046:Gold