title: Interaction of the neurotransmitter, neuropeptide Y, with phospholipid membranes: infrared spectroscopic characterization at the air/water interface.
abstract: The association of neuropeptide Y (NPY) at the air/water interface and with phospholipid monolayers on water as subphase has been investigated using external infrared reflection absorption spectroscopy (IRRAS). Studies of the conformation and orientation of NPY suggest that it adopts an alpha-helical structure and is oriented parallel to the air/water interface in neat peptide monolayers. Both secondary structure and orientation are preserved in mixed lipid/NPY monolayers. Comparison of NPY associated with zwitterionic DPPC and with anionic DMPS suggests that electrostatic attraction plays a major role for peptide binding to the membrane surface.
authors.affiliation: University of Leipzig, Institute of Experimental Physics I, D-04103 Leipzig, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Dyck
authors.forename: Andreas
authors.initials: A
authors.lastname: Kerth
authors.forename: Alfred
authors.initials: A
authors.lastname: Blume
authors.forename: Mathias
authors.initials: M
authors.lastname: Lösche
mesh_terms: D008567:Membranes, Artificial; D009478:Neuropeptide Y / Q000737:chemistry* / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D013055:Spectrophotometry, Infrared; D055672:Static Electricity
chemical_list: D008567:Membranes, Artificial; D009478:Neuropeptide Y; D010743:Phospholipids