Identification of a novel murine organic anion transporter like protein 1 (OATLP1) expressed in the kidney.
The organic anion transporters (OATs) are expressed in various tissues, primarily in the kidney and liver, but they are also expressed in the placenta, small intestine, and the choroid plexus, which are all epithelial tissues that transport xenobiotics. Six isoforms of OATs are currently known. Considering the variety of organic anionic compounds, other OATs isoforms can be assumed. In this connection, we have searched for a new isoform in the expressed sequence tag (EST) database. We found the new candidate clone AK052752 in the mouse kidney cDNA library and we named it mouse organic anion transporter like protein 1 (mOATLP1). The mOATLP1 cDNA consisted of 2221 base pairs that encoded a 552 amino acid residue protein with 12 putative transmembrane domains. The deduced amino acid sequence of mOATLP1 showed 37 to 63% identity to other members of the OAT family. According to the tissue distribution based on Northern blot analysis, 2.7 kb and 2.9 kb mOATLP1 transcripts (approximate sizes) were observed in the kidney and liver. An 85-kDa band (approximate) was detected using Western blot analysis of mouse kidney performed with a synthesized oligopeptide-induced mOATLP1 antibody. Immunohistochemical results showed mOATLP1 was stained in the blood vessels, glomeruli (the parietal epithelial cells and podocytes), distal convoluted tubules, connecting tubules, and inner medullary collecting tubules. mOATLP1 appears to be a novel candidate for an organic anion transporter isoform identified in the kidney.
Jung
Sun-Mi
SM
Department of Pharmacology and Toxicology, and Center for Advanced Medical Education by BK21 Project, Inha University, Incheon 400-103, Korea.
Lee
Woon Kyu
WK
Kwak
Jin-Oh
JO
Jung
Sang Yong
SY
Park
Jinyoung
J
Kim
Wan-Young
WY
Kim
Jin
J
Cha
Seok Ho
SH
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D003001:Cloning, Molecular; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009842:Oligopeptides / Q000276:immunology; D027361:Organic Anion Transporters / Q000302:isolation & purification* / Q000378:metabolism*; D010802:Phylogeny; D020033:Protein Isoforms / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D009842:Oligopeptides; D027361:Organic Anion Transporters; D020033:Protein Isoforms; C516873:organic anion transporter like protein 1, mouse