title: Hepatic lipid metabolism changes in short- and long-term prehepatic portal hypertensive rats.
abstract: OBJECTIVE
  To verify the impairment of the hepatic lipid metabolism in prehepatic portal hypertension.
  
  
  METHODS
  The concentrations of free fatty acids, diacylglycerol, triglycerides, and phospholipids were assayed by using D-[U-14C] glucose incorporation in the different lipid fractions and thin-layer chromatography and cholesterol was measured by spectrophotometry, in liver samples of Wistar rats with partial portal vein ligation at short- (1 mo) and long-term (1 year) (i.e. portal hypertensive rats) and the control rats.
  
  
  RESULTS
  In the portal hypertensive rats, liver phospholipid synthesis significantly decreased (7.42 +/- 0.50 vs 4.70 +/- 0.44 nCi/g protein; P < 0.01) and was associated with an increased synthesis of free fatty acids (2.08 +/- 0.14 vs 3.36 +/- 0.33 nCi/g protein; P < 0.05), diacylglycerol (1.93 +/- 0.2 vs 2.26 +/- 0.28 nCi/g protein), triglycerides (2.40 +/- 0.30 vs 4.49 +/- 0.15 nCi/g protein) and cholesterol (24.28 +/- 2.12 vs 57.66 +/- 3.26 mg/g protein; P < 0.01).
  
  
  CONCLUSIONS
  Prehepatic portal hypertension in rats impairs the liver lipid metabolism. This impairment consists in an increase in lipid deposits (triglycerides, diacylglycerol and cholesterol) in the liver, accompanied by a decrease in phospholipid synthesis.
authors.affiliation: Surgery I Department, School of Medicine, Complutense University of Madrid, Spain. maaller@med.ucm.es
authors.forename: Maria-Angeles
authors.initials: MA
authors.lastname: Aller
authors.forename: Elena
authors.initials: E
authors.lastname: Vara
authors.forename: Cruz
authors.initials: C
authors.lastname: García
authors.forename: Maria-Paz
authors.initials: MP
authors.lastname: Nava
authors.forename: Alejandra
authors.initials: A
authors.lastname: Angulo
authors.forename: Fernando
authors.initials: F
authors.lastname: Sánchez-Patán
authors.forename: Ana
authors.initials: A
authors.lastname: Calderón
authors.forename: Patri
authors.initials: P
authors.lastname: Vergara
authors.forename: Jaime
authors.initials: J
authors.lastname: Arias
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D002784:Cholesterol / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006975:Hypertension, Portal / Q000378:metabolism* / Q000503:physiopathology; D050356:Lipid Metabolism / Q000502:physiology*; D008099:Liver / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D008102:Liver Circulation / Q000502:physiology; D008297:Male; D009929:Organ Size / Q000502:physiology; D010743:Phospholipids / Q000378:metabolism; D011169:Portal Vein / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005230:Fatty Acids, Nonesterified; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol