Intralipid minimizes hepatocytes injury after anoxia-reoxygenation in an ex vivo rat liver model.
OBJECTIVE
Ischemia-reperfusion injury is a determinant in liver injury occurring during surgical procedures, ischemic states, and multiple organ failure. The pre-existing nutritional status of the liver, i.e., fasting, might contribute to the extent of tissue injury. This study investigated whether Intralipid, a solution containing soybean oil, egg phospholipids, and glycerol, could protect ex vivo perfused livers of fasting rats from anoxia-reoxygenation injury.


METHODS
The portal vein was cannulated, and the liver was removed and perfused in a closed ex vivo system. Isolated livers were perfused with glucose 5.5 and 15 mM, and two different concentrations of Intralipid, i.e., 0.5:100 and 1:100 (v/v) Intralipid 10%:medium (n = 5 in each group). The experiment consisted of perfusion for 15 min, warm anoxia for 60 min, and reoxygenation during 60 min. Hepatic enzymes, potassium, glucose, lactate, bilirubin, dienes, trienes, and cytochrome-c were analyzed in perfusate samples. The proportion of glycogen in hepatocytes was determined in biopsies.


RESULTS
Intralipid attenuated transaminases, lactate dehydrogenase, potassium, diene, and triene release in the perfusate (dose-dependant) during the reoxygenation phase when compared with glucose-treated groups. The concentration of cytochrome-c in the medium was the highest in the 5.5-mM glucose group. The glycogen content was low in all livers at the start of the experiment.


CONCLUSIONS
Intralipid presents, under the present experimental conditions, a better protective effect than glucose in anoxia-reoxygenation injury of the rat liver.
Stadler
Michaela
M
Department of Anesthesiology, University Hospital Center, Charleroi, Belgium. michaela.stadler@chu-charleroi.be
Nuyens
Vincent
V
Boogaerts
Jean G
JG
D000818:Animals; D001663:Bilirubin / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005215:Fasting; D005217:Fat Emulsions, Intravenous / Q000494:pharmacology*; D005260:Female; D006498:Hepatectomy; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000293:injuries* / Q000473:pathology; D008112:Liver Glycogen / Q000378:metabolism; D009752:Nutritional Status; D009924:Organ Culture Techniques; D010100:Oxygen / Q000378:metabolism*; D010477:Perfusion; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*
D001786:Blood Glucose; D005217:Fat Emulsions, Intravenous; D008112:Liver Glycogen; D045304:Cytochromes c; D001663:Bilirubin; D010100:Oxygen