Novel noncoding RNA from human Y distal heterochromatic block (Yq12) generates testis-specific chimeric CDC2L2.
The human Y chromosome, because it is enriched in repetitive DNA, has been very intractable to genetic and molecular analyses. There is no previous evidence for developmental stage- and testis-specific transcription from the male-specific region of the Y (MSY). Here, we present evidence for the first time for a developmental stage- and testis-specific transcription from MSY distal heterochromatic block. We isolated two novel RNAs, which localize to Yq12 in multiple copies, show testis-specific expression, and lack active X-homologs. Experimental evidence shows that one of the above Yq12 noncoding RNAs (ncRNAs) trans-splices with CDC2L2 mRNA from chromosome 1p36.3 locus to generate a testis-specific chimeric beta sv13 isoform. This 67-nt 5'UTR provided by the Yq12 transcript contains within it a Y box protein-binding CCAAT motif, indicating translational regulation of the beta sv13 isoform in testis. This is also the first report of trans-splicing between a Y chromosomal and an autosomal transcript.
Jehan
Zeenath
Z
Centre for Cellular and Molecular Biology, Uppal Road Hyderabad-500 007, AP, India.
Vallinayagam
Sambandam
S
Tiwari
Shrish
S
Pradhan
Suman
S
Singh
Lalji
L
Suresh
Amritha
A
Reddy
Hemakumar M
HM
Ahuja
Y R
YR
Jesudasan
Rachel A
RA
D000328:Adult; D001483:Base Sequence; D015152:Blotting, Northern; D041322:Chromosomes, Human, Y / Q000235:genetics*; D018844:Cyclin-Dependent Kinases / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006570:Heterochromatin / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic
D006570:Heterochromatin; D020033:Protein Isoforms; D022661:RNA, Untranslated; C447612:CDK11a protein, human; D018844:Cyclin-Dependent Kinases