title: A novel keratinase from Clostridium sporogenes bv. pennavorans bv. nov., a thermotolerant organism isolated from solfataric muds.
abstract: A bacterium which can grow on chicken feathers and which exhibits keratinolytic activity was isolated from solfataric muds. It was classified as belonging to the genus Clostridium and closely related to C. sporogenes. Based on its unique capability to degrade chicken feathers, it was designated as Clostridium sporogenes bv. pennavorans bv. nov. The keratinase purified from the culture supernatant is a monomer of 28.7kDa molecular mass. The enzyme is relatively thermostable and is active over a broad range of temperature and pH. Specific enzymatic assays demonstrate that keratinase can act on a large variety of soluble and insoluble protein substrates.
authors.affiliation: Institute of Protein Biochemistry--CNR, Naples, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Ionata
authors.forename: F
authors.initials: F
authors.lastname: Canganella
authors.forename: G
authors.initials: G
authors.lastname: Bianconi
authors.forename: Y
authors.initials: Y
authors.lastname: Benno
authors.forename: M
authors.initials: M
authors.lastname: Sakamoto
authors.forename: A
authors.initials: A
authors.lastname: Capasso
authors.forename: M
authors.initials: M
authors.lastname: Rossi
authors.forename: F
authors.initials: F
authors.lastname: La Cara
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002645:Chickens; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D017077:Culture Media, Conditioned / Q000378:metabolism; D005241:Feathers; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007558:Italy; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010447:Peptide Hydrolases; C035855:keratinase