Preimplantation and fetal development of mouse embryos cultured in a protein-free, chemically defined medium.
Mouse two-cell embryos of block (ICR) or nonblock (F1 of C57BL x DBA) strains were cultured in modified Chatot, Ziomek, and Bavister medium, supplemented with bovine serum albumin or polyvinyl alcohol, a synthetic macromolecule. The supplementation did not influence morula compaction and blastocyst formation, mean cell numbers of total blastomeres, inner cell mass (ICM) cells and trophectodermal (TE) cells in blastocysts, rates of pregnancy and delivery, mean litter size, number of implantation spots in pregnant females, and the expression of several genes related to pluripotency, organogenesis, and implantation.
Jang
Mi
M
Department of Food and Animal Biotechnology, Seoul National University, Seoul, South Korea.
Lee
Eun J
EJ
Lee
Seung Tae
ST
Cho
Meeyoung
M
Lim
Jeong M
JM
D000818:Animals; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology*; D005240:Feasibility Studies; D005260:Female; D047109:Fetal Development / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D009924:Organ Culture Techniques / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism
D003470:Culture Media; D011506:Proteins