title: Mycobacterium tuberculosis malate synthase- and MPT51-based serodiagnostic assay as an adjunct to rapid identification of pulmonary tuberculosis.
abstract: The 81-kDa malate synthase (MS; Rv 1837c) and the 27-kDa MPT51 (Rv 3803c) of Mycobacterium tuberculosis are immunodominant antigens recognized by serum antibodies from approximately 80% of human immunodeficiency virus-negative smear-positive tuberculosis patients from India. We now provide evidence that the use of the MS/MPT51-based serodiagnostic assay can serve as an adjunct to sputum microscopy in the rapid diagnosis of pulmonary tuberculosis.
authors.affiliation: New York University School of Medicine, New York, New York 10016, USA. jachkar@aecom.yu.edu
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Achkar
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Dong
authors.forename: Robert S
authors.initials: RS
authors.lastname: Holzman
authors.forename: John
authors.initials: J
authors.lastname: Belisle
authors.forename: Irene S
authors.initials: IS
authors.lastname: Kourbeti
authors.forename: Tsering
authors.initials: T
authors.lastname: Sherpa
authors.forename: Rany
authors.initials: R
authors.lastname: Condos
authors.forename: William N
authors.initials: WN
authors.lastname: Rom
authors.forename: Suman
authors.initials: S
authors.lastname: Laal
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D006801:Humans; D008292:Malate Synthase / Q000276:immunology*; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000276:immunology / Q000302:isolation & purification*; D010865:Pilot Projects; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000201:enzymology / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C082911:MPT51 protein, Mycobacterium; D008292:Malate Synthase