title: Differential serum proteomic analysis in a model of metabolic disease.
abstract: Protein profiling would aid in better understanding the pathophysiology of metabolic disease. Here, we report on differential proteomic analysis using an animal model of diabetes mellitus and associated metabolic disorders (Otsuka Long-Evans Tokushima Fatty rat). Serum was analyzed by a new two-dimensional liquid chromatography system which separated proteins by chromatofocusing and subsequent reversed-phase chromatography. This is the first application of this approach to differential serum proteomics. Differentially expressed proteins, identified with MALDI-TOF mass spectrometry, included apolipoproteins and alpha2-HS-glycoprotein. These findings add to our understanding of the underlying pathophysiology. This new proteomic analysis is a promising tool to elucidate disease mechanisms.
authors.affiliation: Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Matsumura
authors.forename: Toru
authors.initials: T
authors.lastname: Suzuki
authors.forename: Nanae
authors.initials: N
authors.lastname: Kada
authors.forename: Kenichi
authors.initials: K
authors.lastname: Aizawa
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Munemasa
authors.forename: Ryozo
authors.initials: R
authors.lastname: Nagai
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D015153:Blotting, Western; D002853:Chromatography, Liquid / Q000379:methods*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004195:Disease Models, Animal; D006023:Glycoproteins / Q000032:analysis; D008297:Male; D008659:Metabolic Diseases / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D020318:Rats, Long-Evans; D011972:Receptor, Insulin / Q000037:antagonists & inhibitors; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001053:Apolipoproteins; D001798:Blood Proteins; D006023:Glycoproteins; D020543:Proteome; D011972:Receptor, Insulin