title: Determination of glucosinolates in traditional Chinese herbs by high-performance liquid chromatography and electrospray ionization mass spectrometry.
abstract: A reversed-phase HPLC method has been developed for determination of twelve intact glucosinolates--glucoiberin, glucocheirolin, progoitrin, sinigrin, epiprogoitrin, glucoraphenin, sinalbin, gluconapin, glucosibarin, glucotropaeolin, glucoerucin, and gluconasturtiin--in ten traditional Chinese plants. The samples were extracted with methanol and the extracts were cleaned on an activated Florisil column. A mobile phase gradient prepared from methanol and 30 mmol L(-1) ammonium acetate at pH 5.0 enabled baseline separation of the glucosinolates. Glucosinolate detection was confirmed by quadrupole time-of-flight tandem mass spectrometric analysis in negative-ionization mode. Detection limits ranged from 0.06 to 0.36 microg g(-1) when 5 g of dried plant was analyzed. Recoveries of the glucosinolates were better than 85% and precision (relative standard derivation, n = 3) ranged from 5.3 to 14.6%. Analysis of the glucosinolates provided scientific evidence enabling differentiation of three pairs of easily confused plants.
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, People's Republic of China.
authors.forename: Kim-Chung
authors.initials: KC
authors.lastname: Lee
authors.forename: Man-Wai
authors.initials: MW
authors.lastname: Cheuk
authors.forename: Wan
authors.initials: W
authors.lastname: Chan
authors.forename: Albert Wai-Ming
authors.initials: AW
authors.lastname: Lee
authors.forename: Zhong-Zhen
authors.initials: ZZ
authors.lastname: Zhao
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Jiang
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D005961:Glucosinolates / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D005961:Glucosinolates; D010936:Plant Extracts