Dykellic acid inhibits cell migration and tube formation by RhoA-GTP expression.
Dykellic acid, a novel factor initially identified from the culture broth of Westerdykella multispora F50733, has been shown to inhibit matrix metalloprotease 9 activity, caspase-3 activity, B cell proliferation and LPS-induced IgM production, suggesting that this factor may have anti-cancer effects. In an effort to further address the possible anti-tumoral effects of dykellic acid, we used wound healing, invasion and RhoA-GTP assays to examine the effects of dykellic acid on cell migration, invasion and angiogenesis. Our results revealed that dykellic acid dose-dependently inhibits B16 cell migration and motility, and inhibits HUVEC tube formation. Western blot analysis of the active form of RhoA (RhoA-GTP) showed that dykellic acid treatment decreased the levels of RhoA-GTP. These findings collectively suggest that dykellic acid may have both anti-metastatic and anti-angiogenic acitivites, and provides the first evidence for the involvement of RhoA in dykellic acid-induced effects.
Heo
Jin-Chul
JC
Department of Food Science & Technology, Kyungpook National University, Daegu, Korea.
Park
Ja-Young
JY
Woo
Sang-Uk
SU
Rho
Jae-Rang
JR
Lee
Ho-Jae
HJ
Kim
Sung-Uk
SU
Kho
Yung-Hee
YH
Lee
Sang-Han
SH
D000818:Animals; D001808:Blood Vessels / Q000187:drug effects* / Q000502:physiology; D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D051379:Mice; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000517:prevention & control; D011422:Propionates / Q000737:chemistry / Q000494:pharmacology*; D011753:Pyrones / Q000737:chemistry / Q000494:pharmacology*; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
D011422:Propionates; D011753:Pyrones; C406051:dykellic acid; D020742:rhoA GTP-Binding Protein