title: Chemico-enzymatic synthesis of a new fluorescent-labeled DNA by PCR with a thymidine nucleotide analogue bearing an acridone derivative.
abstract: Triphosphate of a thymidine analogue bearing acridone was prepared and incorporated as a substrate for PCR using KOD Dash DNA polymerase forming a new fluorescent-labeled DNA that is useful as a DNA probe.
authors.affiliation: Department of Applied Chemistry, Gunma University, Kiryu, Gunma 376-8515, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shoji
authors.forename: Tomoya
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ozaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sawai
mesh_terms: D000166:Acridines / Q000138:chemical synthesis*; D004247:DNA / Q000138:chemical synthesis* / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D009711:Nucleotides / Q000138:chemical synthesis*; D010777:Photochemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet; D013936:Thymidine / Q000031:analogs & derivatives*
chemical_list: D000166:Acridines; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009711:Nucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D013936:Thymidine