Diapause-specific gene expression in the northern house mosquito, Culex pipiens L., identified by suppressive subtractive hybridization.
In this study we probe the molecular events underpinning diapause observed in overwintering females of Culex pipiens. Using suppressive subtractive hybridization (SSH) we have identified 40 genes that are either upregulated or downregulated during this seasonal period of dormancy. Northern blot hybridizations have confirmed the expression of 32 of our SSH clones, including six genes that are upregulated specifically in early diapause, 17 that are upregulated in late diapause, and two upregulated throughout diapause. In addition, two genes are diapause downregulated and five remain unchanged during diapause. These genes can be categorized into eight functional groups: genes with regulatory functions, metabolically-related genes, those involved in food utilization, stress response genes, cytoskeletal genes, ribosomal genes, transposable elements, and genes with unknown functions.
Robich
Rebecca M
RM
Department of Entomology, Ohio State University, 318 West 12th Avenue, Columbus, OH 43210, USA.
Rinehart
Joseph P
JP
Kitchen
Linda J
LJ
Denlinger
David L
DL
D000818:Animals; D015152:Blotting, Northern; D019295:Computational Biology; D003465:Culex / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006605:Hibernation / Q000502:physiology*; D019476:Insect Proteins / Q000378:metabolism*; D009693:Nucleic Acid Hybridization / Q000379:methods; D009820:Ohio
D019476:Insect Proteins