Preclinical investigations of a medium-chain triglyceride:fish oil emulsion III. Experiments in cultured endothelial cells.
The bolus intravenous injection of a novel medium-chain triglyceride:fish oil emulsion was recently reported to provoke a rapid and sustained increase in the cell phospholipid content of long-chain polyunsaturated omega3 fatty acids in both rats and human subjects. This report deals mainly with a comparison between this and other emulsions, as well as albumin-bound omega3 fatty acids, in terms of the time course, reversibility and concentration dependency for the incorporation of the omega3 fatty acids in the phospholipids of cultured endothelial cells. The results document that the new emulsion is quite efficient for a rapid and sustained enrichment of phospholipids in long-chain polyunsaturated omega3 fatty acids. The potential beneficial effects of such an enrichment in terms of aortic endothelial and cardiac function are emphasized.
Carpentier
Yvon A
YA
Laboratory of Experimental Surgery, Brussels Free University, B-1070 Brussels, Belgium.
Dupont
Isabelle
I
Portois
Laurence
L
Malaisse
Willy J
WJ
D000818:Animals; D001011:Aorta / Q000166:cytology; D002417:Cattle; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D004655:Emulsions / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000032:analysis; D005395:Fish Oils / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008892:Milk / Q000201:enzymology; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014280:Triglycerides / Q000737:chemistry / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D004655:Emulsions; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D010743:Phospholipids; D014280:Triglycerides; D012710:Serum Albumin, Bovine