title: Assessment of coenzyme Q10 absorption using an in vitro digestion-Caco-2 cell model.
abstract: The feasibility of using a coupled in vitro digestion-Caco-2 cell uptake as a model for examining the digestive stability and absorption of coenzyme Q10 (CoQ10) from a variety of commercially available CoQ10 products was examined. The products were first subjected to simulated digestion to mimic their passage through the GI tract to generate micelles containing CoQ10, and the micelle fractions added to monolayers of Caco-2 cells to determine CoQ10 uptake. The data demonstrate enhanced uptake of CoQ10 from formulations containing solubilized forms of CoQ10 and also from a CoQ10-gamma-cyclodextrin complex as compared with pure CoQ10 powder or tablets based on CoQ10 powder. The CoQ10 uptake by the cells was correlated with the extent of micellarization of CoQ10 during simulated digestion. Most of CoQ10 taken up by the cells was converted to ubiquinol either during or following uptake. The data also indicate a correlation between in vitro dissolution of CoQ10 products and uptake of CoQ10 by Caco-2 cells. Thus, this study demonstrates the utility of coupled in vitro digestion-Caco-2 cell model as a cost-effective screening tool that will provide useful information for the optimal design of human trials to assess the bioavailability of CoQ10 and also other bioactive compounds.
authors.affiliation: Tishcon Corporation, Westbury, NY 11590, USA. hemmin@msn.com
authors.forename: Hemmi N
authors.initials: HN
authors.lastname: Bhagavan
authors.forename: Raj K
authors.initials: RK
authors.lastname: Chopra
authors.forename: Neal E
authors.initials: NE
authors.lastname: Craft
authors.forename: Chureeporn
authors.initials: C
authors.lastname: Chitchumroonchokchai
authors.forename: Mark L
authors.initials: ML
authors.lastname: Failla
mesh_terms: D001682:Biological Availability; D001711:Biotransformation; D018938:Caco-2 Cells; D002626:Chemistry, Pharmaceutical; D003067:Coenzymes; D004063:Digestion; D004355:Drug Stability; D005079:Excipients / Q000737:chemistry; D005240:Feasibility Studies; D006801:Humans; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D008823:Micelles; D011208:Powders; D012995:Solubility; D013607:Tablets; D013997:Time Factors; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D014815:Vitamins / Q000737:chemistry / Q000378:metabolism*; D047408:gamma-Cyclodextrins / Q000737:chemistry
chemical_list: D003067:Coenzymes; D005079:Excipients; D008823:Micelles; D011208:Powders; D013607:Tablets; D014815:Vitamins; D047408:gamma-Cyclodextrins; D014451:Ubiquinone; C024989:coenzyme Q10; C003741:ubiquinol