[RelB gene expression in murine bone marrow-derived mature and immature dendritic cells].
OBJECTIVE
To explore the expression of avian reticuloendotheliosis viral(v-rel) oncogene-related B (RelB) mRNA in vitro in murine mature and immature myeloid dendritic cells (DCs).


METHODS
The bone marrow was collected from the femur and tibias of C57BL/6 mice in sterile condition. Bone marrow precursors were cultured with recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) and interleukin-4 (rmIL-4) to produce immature DCs. Immature DCs were stimulated by LPS 18 h before the end of culture to become mature. DCs' phenotype was detected by flow cytometry (FCM). RelB expression and protein level were determined by RT-PCR and immunofluorescence staining.


RESULTS
The expression level of co-stimulatory molecules (CD86 and CD40) and MHC-II class molecule were low in immature DCs, whereas high in mature DCs. RelB expression was significantly higher in mature DCs than in immature DCs (P<0.01).


CONCLUSIONS
RelB expression is closely associated with the maturative situation of DCs. Inhibition of RelB expression in DCs may induce tolerogenic DCs.
Bao
Jie
J
Laboratory Medicine Center, Nangfang Hospital, Southern Medical University, Guangzhou 510515, China. baojiemimi@163.com
Zheng
Lei
L
Wang
Qian
Q
Gu
Chun-Yu
CY
Qiu
Yu-Rong
YR
Li
Juan
J
D000199:Actins / Q000235:genetics; D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology / Q000628:therapy; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology*; D019013:CD40 Antigens / Q000235:genetics; D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D007108:Immune Tolerance / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051841:Transcription Factor RelB / Q000235:genetics*
D000199:Actins; D019013:CD40 Antigens; D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; D047888:Receptors, Tumor Necrosis Factor, Type I; C497594:Relb protein, mouse; D051841:Transcription Factor RelB