Rapid expression of Mycobacterium avium subsp. paratuberculosis recombinant proteins for antigen discovery.
Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease, a chronic granulomatous enteritis of ruminants and other species. Detection of infection in animals is hampered by the lack of sensitive and specific diagnostic assays. We describe here an approach that utilizes translationally active PCR fragments for the rapid in vitro transcription and translation of recombinant proteins for antigen discovery in M. avium subsp. paratuberculosis. The investigations showed that the MAP1272c protein selectively reacts with sera from Johne's disease-positive cattle and represents an antigen of potential utility in M. avium subsp. paratuberculosis immunodiagnostics.
Li
Lingling
L
Department of Microbiology, University of Minnesota, 1500 Gortner Ave., St. Paul, MN 55108, USA.
Munir
Shirin
S
Bannantine
John P
JP
Sreevatsan
Srinand
S
Kanjilal
Sagarika
S
Kapur
Vivek
V
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology / Q000378:metabolism*; D010283:Paratuberculosis / Q000175:diagnosis; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism
D000942:Antigens, Bacterial; D011993:Recombinant Fusion Proteins