B-1 and B-2 B-cells in the pig cannot be differentiated by expression of CD5.
In a number of species, such as mice, humans and cattle, B-cells can be differentiated into two populations based on the surface expression of CD5, a marker normally found on T-cells. These B-cell subsets have been found to differ with regard to location, development and phenotypic characteristics. The B-1 (CD5(+)) B-cells have also been shown to have a more restricted immunoglobulin isotype expression profile, limited combinatorial diversity in immunoglobulin heavy chains and lower somatic hyper-mutation. They are potent producers of IL-10. In the pig, CD5(+) and CD5(-) B-cell populations have previously been described in this laboratory. Here, we show that B-cells isolated and separated into CD5(+) and CD5(-) populations do not differ with regard to immunoglobulin isotype or IL-10 RNA expression, nor do the immunoglobulin heavy chain V(D)J re-arrangements differ in terms of gene usage, CDR3 length and composition or the frequency of hyper-mutations. In conclusion, expression of CD5 cannot be used to differentiate between pig blood B-1 and B-2 B-cells.
Wilson
Stephen M
SM
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ont. N1G 2W1, Canada. s_m_wilson@hotmail.com
Wilkie
Bruce N
BN
D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry* / Q000145:classification; D018956:CD5 Antigens / Q000032:analysis*; D005434:Flow Cytometry; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007132:Immunoglobulin Isotypes / Q000235:genetics; D016753:Interleukin-10 / Q000032:analysis; D013552:Swine / Q000276:immunology*
D018956:CD5 Antigens; D007143:Immunoglobulin Heavy Chains; D007132:Immunoglobulin Isotypes; D016753:Interleukin-10