Endogenous cholecystokinin reduces food intake and increases Fos-like immunoreactivity in the dorsal vagal complex but not in the myenteric plexus by CCK1 receptor in the adult rat.
We hypothesized that endogenous CCK reduces food intake by activating the dorsal vagal complex (DVC) and the myenteric neurons of the gut. To test this hypothesis, adult rats were given camostat mesilate; a nonnutrient releaser of endogenous CCK, by orogastric gavage, and Fos-like immunoreactivity (Fos-LI) was quantified in the DVC and the myenteric plexus. The results for endogenous CCK were compared with those for exogenous CCK-8. Exogenous CCK-8 reduced food intake and stimulated Fos-LI in the DVC and in myenteric neurons of the duodenum and jejunum. In comparison, endogenous CCK reduced food intake and increased DVC Fos-LI but did not increase Fos-LI in the myenteric plexus. Similar to CCK-8, devazepide, a specific CCK(1) receptor antagonist, and not L365,260, a specific CCK(2) receptor antagonist, attenuated the reduction of food intake by camostat. In addition, Fos-LI in the DVC in response to both exogenous CCK-8 and camostat administration was significantly attenuated by vagotomy, as well as by blocking CCK(1) receptors. These results demonstrate for the first time that reduction of food intake in adult rats by endogenous CCK released by a nonnutrient mechanism requires CCK(1) receptors, the vagus nerve, and activation of the DVC, but not the myenteric plexus.
Sullivan
Cherese N
CN
Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL 36088, USA.
Raboin
Shannon J
SJ
Gulley
Stephen
S
Sinzobahamvya
Ntwenzi T
NT
Green
Gary M
GM
Reeve
Joseph R
JR
Sayegh
Ayman I
AI
D000818:Animals; D031608:Area Postrema / Q000187:drug effects / Q000378:metabolism; D002766:Cholecystokinin / Q000494:pharmacology / Q000502:physiology*; D004435:Eating / Q000187:drug effects / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008954:Models, Biological; D009197:Myenteric Plexus / Q000166:cytology* / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011949:Receptors, Cholecystokinin / Q000502:physiology*; D017552:Solitary Nucleus / Q000187:drug effects / Q000378:metabolism; D014630:Vagus Nerve / Q000187:drug effects / Q000378:metabolism*; D000099169:Fos-Related Antigen 1
D002766:Cholecystokinin; D016760:Proto-Oncogene Proteins c-fos; D011949:Receptors, Cholecystokinin; D000099169:Fos-Related Antigen 1