title: Homogeneous osteogenesis and bone regeneration by demineralized bone matrix loading with collagen-targeting bone morphogenetic protein-2.
abstract: Considerable research has been focused on the development of bone morphogenetic protein-2 (BMP-2) delivery system for homologous and efficient bone regeneration. The aim of the present study was to develop a collagen-based targeting bone repair system. A collagen-binding domain (CBD) was added to the N-terminal of native BMP-2 to allow it bind to collagen specifically. We showed that the collagen-binding bone morphogenetic protein-2 (named bone morphogenetic protein2-h, BMP2-h) had maintained the full biological activity as compared to rhBMP2 lacking the CBD. In vitro functional study also demonstrated that collagen matrix could maintain higher bioactivity of BMP2-h than native BMP-2. When demineralized bone matrix (DBM) impregnated with BMP2-h was implanted subcutaneously in rats, homogeneous bone formation was observed. Moreover, in a rabbit mandible defect model, surgical implantation of collagen matrix loaded with BMP2-h exhibited remarkable osteoinductive properties and excellent homogeneous bone formation. Our studies suggested that this novel collagen-based BMP-2 targeting bone repair system induced better bone formation not only in quantity but also in quality. Similar approaches may also be used for the repair of other tissue injuries.
authors.affiliation: Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, Beijing 100080, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Hang
authors.initials: H
authors.lastname: Lin
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.forename: Yannan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Wenxue
authors.initials: W
authors.lastname: Zhao
authors.forename: Wenjie
authors.initials: W
authors.lastname: Sun
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000737:chemistry*; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D001861:Bone Regeneration / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry; D017102:Fracture Healing / Q000187:drug effects; D008297:Male; D008337:Mandibular Fractures / Q000188:drug therapy* / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D016896:Treatment Outcome
chemical_list: C525821:Bmp7 protein, rat; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; D016212:Transforming Growth Factor beta; D003094:Collagen