A new chemical probe for the detection of the cancer-linked galectin-3.
A chemical probe was developed for the detection of the emerging cancer marker galectin-3. The probe contains a benzophenone moiety which covalently attaches itself to the protein upon binding and irradiation. Introduction of a fluorescent label via'click' chemistry allows the labelled proteins to be visualized in a gel. With the probe, selective visualization of galectin-3 in protein mixtures was shown and remarkably even in cell lysates.
Ballell
Lluis
L
Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80082, 3508 TB, Utrecht, The Netherlands.
van Scherpenzeel
Monique
M
Buchalova
Katerina
K
Liskamp
Rob M J
RM
Pieters
Roland J
RJ
D014408:Biomarkers, Tumor / Q000032:analysis*; D018938:Caco-2 Cells / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D037502:Galectin 3 / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry
D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D037502:Galectin 3; D007202:Indicators and Reagents