title: Potential role of estrogen receptor alpha (ERalpha) phosphorylated at Serine118 in human breast cancer in vivo.
abstract: Post-translational modifications of proteins are known to be important in protein activity and ERalpha is known to be phosphorylated at multiple sites within the protein. The exact function of site-specific phosphorylation in ERalpha is unknown, although several hypotheses have been developed using site-directed mutagenesis and cell culture models. Targeting the ERalpha at the level of such post-translational modification pathways would be a new and exciting approach to endocrine therapy in breast cancer, but adequate knowledge is lacking with regard to the relevance of site-specific phosphorylation in ERalpha in human breast cancer in vivo. Recently, antibodies to P-Serine(118)-ERalpha and P-Serine(167)-ERalpha, two major sites of phosphorylation in ERalpha, have become available and some in vivo data are now available to complement studies in cells in culture. However, the in vivo data are somewhat contradictory and limited by the small cohorts used and the lack of standard well-characterized reagents and protocols.
authors.affiliation: Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Man. R3E 0V9, Canada. lcmurph@cc.umanitoba.ca
authors.forename: Leigh C
authors.initials: LC
authors.lastname: Murphy
authors.forename: G E
authors.initials: GE
authors.lastname: Weitsman
authors.forename: G P
authors.initials: GP
authors.lastname: Skliris
authors.forename: E M
authors.initials: EM
authors.lastname: Teh
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Peng
authors.forename: J R
authors.initials: JR
authors.lastname: Davie
authors.forename: K
authors.initials: K
authors.lastname: Ung
authors.forename: Y-L
authors.initials: YL
authors.lastname: Niu
authors.forename: S
authors.initials: S
authors.lastname: Troup
authors.forename: L
authors.initials: L
authors.lastname: Tomes
authors.forename: P H
authors.initials: PH
authors.lastname: Watson
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D010766:Phosphorylation; D012694:Serine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D047628:Estrogen Receptor alpha; D012694:Serine