title: Combined effect of 25-hydroxycholesterol and IL-1beta on IL-8 production in human colon carcinoma cell line (Caco-2).
abstract: Interleukin-1 beta (IL-1beta) is an important mediator in intestinal inflammation. IL-1beta promotes IL-8 production, which can be modulated by a number of factors, including oxidative stress. Interestingly, oxysterols, which are thought to contribute to inflammation in atherosclerotic plaques, are also produced by intestinal epithelial cells. Thus, we investigated the effect of oxysterols, including 25-hydroxycholesterol and 7beta-hydroxycholesterol, on IL-1beta-induced IL-8 production in Caco-2 cells (a human colon carcinoma cell line). Pre-treatment of Caco-2 cells with 25-hydroxycholesterol significantly enhanced IL-1beta-induced IL-8 expression at both mRNA and protein levels. However, 7beta-hydroxycholesterol showed very little effect on IL-8 production. Furthermore, pre-treatment with 25-hydroxycholesterol, followed by IL-1beta stimulation, enhanced IL-8 promoter activity beyond that observed with IL-1beta alone. These results suggest that 25-hydroxycholesterol enhances IL-1beta-induced IL-8 production, possibly by enhancing promoter activity.
authors.affiliation: Department of Dermatology, Shiga University of Medical Science, Seta-Tsukinowa, Otsu, Shiga 520-2192, Japan.
authors.forename: Bingxue
authors.initials: B
authors.lastname: Bai
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sato
authors.forename: Hisashi
authors.initials: H
authors.lastname: Sugiura
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D018938:Caco-2 Cells; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006801:Humans; D006888:Hydroxycholesterols / Q000008:administration & dosage / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000494:pharmacology*; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009994:Osmolar Concentration; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D006888:Hydroxycholesterols; D053583:Interleukin-1beta; D016209:Interleukin-8; D012333:RNA, Messenger; C011724:cholest-5-en-3 beta,7 alpha-diol; C007997:25-hydroxycholesterol