LY-6K gene: a novel molecular marker for human breast cancer.
A full-length cDNA was identified using one STS sequence containing an SNP (single nucleotide polymorphism) derived from genomic DNAs of breast cancer patients using a variety of bioinformatics tools. The cDNA encodes LY-6K, a novel member protein of the Ly-6/uPAR superfamily. It has been annotated as a target antigen for the HNSCC (head-and neck squamous cell carcinoma). We isolated the LY-6K gene from genomic DNAs obtained from breast cancer patients through large scale, case-control-screening. We performed northern blot hybridization and semi-quantitative RT-PCR on a human multiple-tissue mRNA blot from several breast cancer patients. We investigated the expression level of the LY-6K gene in human breast cancer, and compared this to expression in human normal breast tissue. We found that LY-6K was more highly expressed in the mRNA of breast tumors compared to its expression in normal breast tissue. These results suggest that LY-6K is not only a target antigen for HNSCC but also a significant new molecular marker for diagnosis and gene therapy in patients with breast cancer.
Lee
Ji Woo
JW
Department of Biological Science, Sookmyung Women's University, Seoul 140-742, Korea.
Lee
Yeon Su
YS
Yoo
Kyung Hyun
KH
Lee
Kee Ho
KH
Park
Kyunghee
K
Ahn
Taejin
T
Ko
Christopher
C
Park
Jong Hoon
JH
D000950:Antigens, Ly / Q000096:biosynthesis* / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000032:analysis*; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000950:Antigens, Ly; D014408:Biomarkers, Tumor