Preventive effect of naringin on lipid peroxides and antioxidants in isoproterenol-induced cardiotoxicity in Wistar rats: biochemical and histopathological evidences.
This study was designed to evaluate the cardioprotective potential of naringin on lipid peroxides, enzymatic and nonenzymatic antioxidants and histopathological findings in isoproterenol (ISO)-induced myocardial infarction (MI) in rats. Subcutaneous injection of ISO (85 mg/kg) to male Wistar rats showed a significant increase in the levels of thiobarbituric acid reactive substances and lipid hydroperoxides in plasma and the heart and a significant decrease in the activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase in the heart and the levels of reduced glutathione, vitamin C and vitamin E in plasma and heart and ceruloplasmin in plasma. Oral administration of naringin (10, 20 and 40 mg/kg, respectively) to ISO-induced rats daily for a period of 56 days showed a significant decrease in the levels of lipid peroxidative products and improved the antioxidant status by increasing the activities of antioxidant enzymes and nonenzymatic antioxidants. Histopathological findings of the myocardial tissue showed the protective role of naringin in ISO-induced rats. The effect at a dose of 40 mg/kg of naringin was more pronounced than that of the other two doses, 10 and 20mg/kg. The results of our study show that naringin possess anti-lipoperoxidative and antioxidant activity in experimentally induced cardiac toxicity.
Rajadurai
M
M
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India.
Stanely Mainzen Prince
P
P
D000318:Adrenergic beta-Agonists / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000378:metabolism; D002570:Ceruloplasmin / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D044950:Flavanones / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006331:Heart Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007545:Isoproterenol / Q000633:toxicity*; D050356:Lipid Metabolism / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism*; D008074:Lipoproteins / Q000378:metabolism; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000473:pathology; D009203:Myocardial Infarction / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000318:Adrenergic beta-Agonists; D000975:Antioxidants; D004798:Enzymes; D044950:Flavanones; D008054:Lipid Peroxides; D008074:Lipoproteins; D017392:Thiobarbituric Acid Reactive Substances; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D005982:Glutathione Transferase; D005978:Glutathione; D007545:Isoproterenol; C005274:naringin; D001205:Ascorbic Acid