Studies on PVP hydrogel-supported luminol chemiluminescence: 2. Luminometer calibration and potential analytical applications.
The use of poly(N-vinyl-2-pyrrolidone) (PVP) hydrogel-supported luminol chemiluminescence (CL) for the automatic determination of hydrogen peroxide and the quantification of the antiradical capacity of Trolox is described. The hydrogel containing luminol and hemin is prepared directly on a 96-well microplate and can be stored for up to 3 months without significant decrease in CL quantum yields. Furthermore, this system can also be used as a secondary light standard for the calibration of microplate luminometers.
Bastos
Erick Leite
EL
Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, 05508-900 São Paulo, SP, Brazil.
Ciscato
Luiz Francisco Monteiro Leite
LF
Bartoloni
Fernando Heering
FH
Catalani
Luiz Henrique
LH
Romoff
Paulete
P
Baader
Wilhelm Josef
WJ
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001331:Automation; D002138:Calibration; D002839:Chromans / Q000737:chemistry / Q000494:pharmacology; D006427:Hemin / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D008165:Luminol / Q000737:chemistry*; D015394:Molecular Structure; D011205:Povidone / Q000737:chemistry*
D000975:Antioxidants; D002839:Chromans; D020100:Hydrogels; D008165:Luminol; D006427:Hemin; D006861:Hydrogen Peroxide; D011205:Povidone; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid