title: Homocysteine increases neuronal damage in hippocampal slices receiving oxygen and glucose deprivation.
abstract: Homocystinuria is an inherited metabolic disorder caused by severe deficiency of cystationine beta-synthase activity, resulting in the tissue accumulation of homocysteine (Hcy). Affected patients usually present many signs and symptoms such as seizures, mental retardation, atherosclerosis and stroke. The aim of this study is to evaluate in vivo and in vitro effects of Hcy using hippocampal slices from Wistar rats exposed to oxygen and glucose deprivation (OGD), followed by reoxygenation, an in vitro model of hypoxic-ischemic events. Neural cell injury was quantified by the measurement of lactate dehydrogenase (LDH) released from damaged cells into the extracellular fluid. The results showed that both in vivo and in vitro Hcy increased the LDH released to de incubation medium, suggesting an increase of tissue damage caused by OGD. This fact can be related with the high incidence of stroke in homocystinuric patients.
authors.affiliation: Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Tagliari
authors.forename: Lauren L
authors.initials: LL
authors.lastname: Zamin
authors.forename: Christianne G
authors.initials: CG
authors.lastname: Salbego
authors.forename: Carlos Alexandre
authors.initials: CA
authors.lastname: Netto
authors.forename: Angela T S
authors.initials: AT
authors.lastname: Wyse
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D016923:Cell Death; D005947:Glucose / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006710:Homocysteine / Q000097:blood / Q000494:pharmacology*; D006712:Homocystinuria / Q000378:metabolism* / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009410:Nerve Degeneration / Q000378:metabolism* / Q000473:pathology; D009924:Organ Culture Techniques; D010100:Oxygen / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology
chemical_list: D006710:Homocysteine; D007770:L-Lactate Dehydrogenase; D005947:Glucose; D010100:Oxygen