Targeted chromosome elimination from ES-somatic hybrid cells.
To engineer a stem cell genome, we developed a technology for targeted elimination of chromosomes from mouse embryonic stem (ES)-somatic hybrid cells. Here we demonstrate the use of a universal chromosome elimination cassette (CEC) for elimination of a single embryonic stem cell (ESC)-derived chromosome 11 or 12, and also both copies of chromosome 6, which harbor pluripotency-associated genes including Nanog. We attribute hybrid-cell pluripotency to the expression of Nanog from the reprogrammed somatic-cell nuclei.
Matsumura
Hiroyuki
H
Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Tada
Masako
M
Otsuji
Tomomi
T
Yasuchika
Kentaro
K
Nakatsuji
Norio
N
Surani
Azim
A
Tada
Takashi
T
D000818:Animals; D002872:Chromosome Deletion; D002875:Chromosomes / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D005821:Genetic Techniques; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D006822:Hybrid Cells; D051379:Mice; D000071317:Nanog Homeobox Protein; D013234:Stem Cells
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse