[siRNA inhibits efgl7 expression in human endothelial cell line HUVEC].
OBJECTIVE
To evaluate the effects of small interference RNA (siRNA) on epidermal growth factor-like domain 7 (egfl7) gene expression in human endothelial cell line HUVEC.


METHODS
siRNA targeting egfl7 (siRNA1, siRNA2, siRNA3 and siRNA4) was constructed through online design of Amnion company and transfected into human endothelial cell line HUVEC with lipofectamine. The nontransfected cells and cells treated with control siRNA were taken as controls. At 24, 48 and 72 hours post various interventions, cell viability was determined by MTS method as well as LDH and ATP releasing tests. egfl7 expressions at protein and mRNA levels were detected by Western blot and RT-PCR respectively.


RESULTS
Cell survival rate, LDH and ATP release were significantly reduced in siRNA treated cells compared to control cells (P < 0.05). Similarly, egfl7 expression at protein and mRNA levels was also significantly reduced in siRNA treated cells (P < 0.01), especially in siRNA1 treated cells.


CONCLUSIONS
siRNA inhibited egfl7 gene expression and cell survival in HUVEC.
Jiang
Wei-dong
WD
Department of Cardiology, Second Hospital, School of Medicine, Shandong University, Jinan 250012, China.
Zeng
Ji-ping
JP
Qin
Ai-qiong
AQ
Lu
Qing-hua
QH
Xu
Dong-ling
DL
Ge
Zhi-ming
ZM
D002460:Cell Line; D042783:Endothelial Cells / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D014158:Transcription, Genetic; D014471:Umbilical Veins / Q000166:cytology
D034741:RNA, Small Interfering; D004815:Epidermal Growth Factor