Expression of the self-marker CD47 on dendritic cells governs their trafficking to secondary lymphoid organs.
Dendritic cells (DCs) capture and process Ag in the periphery. Thus, traffic through lymphatic vessels is mandatory before DCs relocate to lymph nodes where they are dedicated to T-cell priming. Here, we show that the ubiquitous self-marker CD47 selectively regulates DC, but not T and B cell trafficking across lymphatic vessels and endothelial barriers in vivo. We find an altered skin DC migration and impaired T-cell priming in CD47-deficient mice at steady state and under inflammatory conditions. Competitive DC migration assays and active immunization with myeloid DCs demonstrate that CD47 expression is required on DCs but not on the endothelium for efficient DC trafficking and T-cell responses. This migratory defect correlates with the quasi-disappearance of splenic marginal zone DCs in nonmanipulated CD47-deficient mice. Nonetheless, CCR7 expression and CCL19-driven chemotaxis remain intact. Our data reveal that CD47 on DCs is a critical factor in controlling migration and efficient initiation of the immune response.
Van
Vu Quang
VQ
Immunoregulation Laboratory, CHUM Research Center, University of Montreal, Montreal, Quebec, Canada.
Lesage
Sylvie
S
Bouguermouh
Salim
S
Gautier
Patrick
P
Rubio
Manuel
M
Levesque
Martin
M
Nguyen
Sébastien
S
Galibert
Laurent
L
Sarfati
Marika
M
D000818:Animals; D051928:CD47 Antigen / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002465:Cell Movement; D002633:Chemotaxis; D003713:Dendritic Cells / Q000737:chemistry / Q000276:immunology*; D042601:Lymphatic Vessels / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D012867:Skin / Q000276:immunology; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D051928:CD47 Antigen