title: Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis.
abstract: New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerpen, Belgium. lrigouts@itg.be
authors.forename: Leen
authors.initials: L
authors.lastname: Rigouts
authors.forename: Oscar
authors.initials: O
authors.lastname: Nolasco
authors.forename: Pim
authors.initials: P
authors.lastname: de Rijk
authors.forename: Elie
authors.initials: E
authors.lastname: Nduwamahoro
authors.forename: Armand
authors.initials: A
authors.lastname: Van Deun
authors.forename: Andy
authors.initials: A
authors.lastname: Ramsay
authors.forename: Jorge
authors.initials: J
authors.lastname: Arevalo
authors.forename: Françoise
authors.initials: F
authors.lastname: Portaels
mesh_terms: D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers; D012321:DNA-Directed RNA Polymerases; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
chemical_list: D000904:Antibiotics, Antitubercular; D001426:Bacterial Proteins; D017931:DNA Primers; C505805:rpoB protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin