title: The effect of erythrodiol-3-acetate on the expressions of matrix metalloproteinase-1 and type-1 procollagen caused by ultraviolet irradiated cultured primary old aged human skin fibroblasts.
abstract: Methanol and aqueous extracts of Styrax japonica used traditionally for the treatments of skin elastic materials were screened in vitro for the matrix metalloproteinase (MMP)-1 inhibitor actions. The methylene chloride soluble fraction of methanol extract from the stems of S. japonica showed significant MMP-1 inhibition in primary old aged human skin fibroblasts caused by ultraviolet (UV) irradiation. Main triterpenoids were isolated by repeated column chromatography. Among them, the triterpenoid erythrodiol-3-acetate reduced the expression of MMP-1 and induced the expression of type-1 procollagen at the protein levels in a dose-dependent manner caused by UV irradiated cultured old aged human skin fibroblasts. Taken together, our results suggest that erythrodiol-3-acetate plays an important role in the skin aging process caused by UV irradiation.
authors.affiliation: Molecular Neuroscience and Vascular Biology Laboratory, Department of Surgery, University of Kentucky Medical Center, Lexington, Kentucky 40536, USA. himun@snu.ac.kr
authors.forename: H-I
authors.initials: HI
authors.lastname: Moon
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002478:Cells, Cultured; D024042:Collagen Type I / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D061965:Matrix Metalloproteinase Inhibitors; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D015595:Skin Aging / Q000528:radiation effects; D032362:Styrax / Q000737:chemistry
chemical_list: D024042:Collagen Type I; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; C517014:erythrodiol-3-acetate; D009828:Oleanolic Acid; D020781:Matrix Metalloproteinase 1