Evaluation of eight RNA isolation methods for transcriptional analysis in Campylobacter jejuni.
DNA-free RNA samples are essential to investigate gene regulation using real-time RT-PCR. This study evaluated eight RNA isolation methods in combination with TURBO DNase treatment and acid phenol extractions for their ability to produce DNA-free RNA from Campylobacter jejuni strains.
Phongsisay
Vongsavanh
V
School of Applied Sciences, Royal Melbourne Institute of Technology University, Melbourne, Budoora, 3083, Victoria, Australia.
Perera
Viraj N
VN
Fry
Benjamin N
BN
D016123:Campylobacter jejuni / Q000235:genetics*; D003851:Deoxyribonucleases / Q000737:chemistry; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet
D012329:RNA, Bacterial; D003851:Deoxyribonucleases