title: [Study of relationship between cyclooxygenase and platelet-activating factor on peripheral blood mononuclear cells in patients with systemic inflammatory response syndrome and multiple organ dysfunction syndrome].
abstract: OBJECTIVE
  To study the role of cyclooxygenase (COX) and platelet-activating factor (PAF) in pathophysiologic mechanisms of patients with systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndrome (MODS).
  
  
  METHODS
  Twenty-eight adult patients whose diagnosis met American college of chest physicians/society of critical care medicine (ACCP/SCCM) criteria for SIRS and MODS were enrolled for study including 13 cases for SIRS group and 15 cases for MODS group. The normal control group consisted of 11 healthy volunteers who matched with study subjects for age and gender. Enzyme linked immunoadsorbent assay (ELISA) was used to measure the content of COX-2 and the activity of platelet-activating factor acetylhydrolase (PAF-AH) of peripheral blood mononuclear cells (PBMCs). Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the COX-2 mRNA and PAF-AH mRNA expression of PBMCs.
  
  
  RESULTS
  The content of COX-2 and the activity of PAF-AH of PBMCs and the expression of their mRNA in MODS group were higher than those in SIRS group and control group (all P<0.05). There was no significant difference between SIRS group and control group. The content of COX-2 and the activity of PAF-AH and the expression of their mRNA of PBMCs in non-survivors were higher than those in survived patients (all P<0.05). In 3 groups, positive correlation was found between the COX-2 content and PAF-AH activity (r=0.329, P<0.05). The leukocyte count, lymphocyte count, and PaO(2)/FiO(2) of peripheral blood in non-survivors showed no significant difference with those of survived patients (all P>0.05). The blood glucose and creatinine of non-survivors were higher than those of survived patients (P<0.05 and P<0.01). The total CO(2) content (TCO(2)) and pH value of non-survivors were lower than those of survived patients (both P<0.01).
  
  
  CONCLUSIONS
  This study shows that COX-2 and PAF-AH play a role in the occurrence of MODS and they can be used as indexes to judge the prognosis of SIRS and MODS. Blood glucose, creatinine, TCO(2) and pH value of blood can be used as other indexes for judging the state and the prognosis of the illness.
authors.affiliation: Department of General Internal Medicine, the First Affiliated Hospital of Sun Yan-sen University, Sun Yan-sen University, Guangzhou 510080, Guangdong, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
authors.forename: Yu-jie
authors.initials: YJ
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Zhan
authors.forename: Yan-wen
authors.initials: YW
authors.lastname: Peng
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Jing
authors.forename: Yan-bing
authors.initials: YB
authors.lastname: Liang
authors.forename: Hai-ning
authors.initials: HN
authors.lastname: Ye
authors.forename: Zhong-fu
authors.initials: ZF
authors.lastname: Ma
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D051546:Cyclooxygenase 2 / Q000097:blood* / Q000235:genetics; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009102:Multiple Organ Failure / Q000097:blood*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood*; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase