title: Molecular cloning of prepro-thyrotropin-releasing hormone cDNA from medaka (Oryzias latipes).
abstract: The cDNA encoding prepro-thyrotropin-releasing hormone (ppTRH) in a teleost, medaka (Oryzias latipes) was isolated and characterized. The medaka ppTRH cDNA codes for 270 amino acid residues including eight TRH progenitor sequences (-Lys/Arg-Arg-Gln-His-Pro-Gly-Lys/Arg-Arg-). In silico analyses of the medaka genome database predicted that the structure of the medaka ppTRH gene is similar to the ppTRH genes of the other vertebrate species studied to date; consisting of three exons and two introns. Identity of the medaka ppTRH with the other vertebrates is rather low except the sockeye salmon. A molecular phylogenic tree showed that the ppTRH sequences reflected the predicted pattern of species classification. RT-PCR analysis demonstrated ppTRH gene expression in the brain and retina. These results gave some insight into the molecular evolution of ppTRH and physiological functions of TRH in vertebrates.
authors.affiliation: Department of Biotechnology, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Aoki
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Masuda
authors.forename: Masayuki
authors.initials: M
authors.lastname: Iigo
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yanagisawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009990:Oryzias / Q000235:genetics*; D010802:Phylogeny; D011498:Protein Precursors / Q000235:genetics*; D012160:Retina / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D013973:Thyrotropin-Releasing Hormone / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D011498:Protein Precursors; C063596:prepro-thyrotropin-releasing hormone; D013973:Thyrotropin-Releasing Hormone