Thermally associating polypeptides designed for drug delivery produced by genetically engineered cells.
Thermally associating polymers, including gelatin, cellulose ethers (e.g., Methocels and poloxamers (e.g., Pluronics) have a long history of use in pharmacy. Over the past 20 years, significant advances in genetic engineering and the understanding of protein secondary and tertiary structures have been made. This has led to the development of a variety of polypeptides that do not occur naturally but can be expressed in recombinant cells and have useful properties that lend themselves to novel applications where current materials cannot perform. The most intensively studied motifs are derived from the consensus repeats of elastin and silk, as well as coiled-coil helices. Many of these designed polypeptides or 'artificial proteins' are thermally associating materials. This property can be exploited to develop solid dosage forms, injectable drug delivery systems, micro- or nanoparticle drug carriers, triggered or targeted release systems, or as a means of simplifying the purification process and thus reducing costs of production of these materials. This review focuses on the development and characterization of this novel class of biomaterials and examines their potential for pharmaceutical applications.
Hart
David S
DS
Department of Chemical and Petroleum Engineering, The University of Kansas, 1530 W 15th St., Lawrence, Kansas 66045, USA.
Gehrke
Stevin H
SH
D000595:Amino Acid Sequence; D000818:Animals; D003094:Collagen / Q000008:administration & dosage; D016503:Drug Delivery Systems; D004549:Elastin / Q000008:administration & dosage / Q000737:chemistry; D005818:Genetic Engineering; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry; D017433:Protein Structure, Secondary; D047011:Silk / Q000008:administration & dosage; D013696:Temperature; D023822:Tissue Engineering
D010455:Peptides; D047011:Silk; D003094:Collagen; D004549:Elastin