Cellulase and protease preparations can extract pectins from various plant byproducts.
The use of protease and cellulase preparations to extract pectins from plant byproducts (chicory, cauliflower) was investigated. Different enzymatic preparations were characterized by their activities toward proteins, cellulose, and pectins. These preparations were then tested regarding pectin extraction, and extraction conditions (nature and concentration of enzyme, incubation time) were optimized. Enzymatic and acidic extractions were compared and also combined in sequential extractions. This study shows that it is possible to extract pectins by using cellulases and proteases. Enzymes can extract pectins with a higher yield ( approximately 35%) than acid (approximately 28%) but enzyme-extracted pectins have a smaller molar mass (300,000 g/mol) than acid-extracted pectins (500,000 g/ mol). Different hypotheses are tested and discussed to explain this mass difference.
Panouillé
Maud
M
INRA-BIA, BP 71627, 44316 Nantes Cedex 3, France.
Thibault
Jean-François
JF
Bonnin
Estelle
E
D001937:Brassica / Q000737:chemistry*; D044602:Cellulases / Q000737:chemistry*; D018651:Cichorium intybus / Q000737:chemistry*; D002850:Chromatography, Gel; D008970:Molecular Weight; D010368:Pectins / Q000737:chemistry / Q000302:isolation & purification*; D010447:Peptide Hydrolases / Q000737:chemistry*
D010368:Pectins; D044602:Cellulases; D010447:Peptide Hydrolases