Gastrokine 1 expression in patients with and without Helicobacter pylori infection.
BACKGROUND
To understand the molecular changes underlying Helicobacter pylori-related gastric diseases is mandatory to prevent gastric cancer. Proteomic technology is providing a rapid expansion of the basic knowledge, particularly in the discovery of new biomarkers involved in the tumourigenesis.


OBJECTIVE
To characterise changes in protein expression level of the gastric mucosa in H. pylori-infected patients.


METHODS
The population enrolled comprised 41 dyspeptic patients. Proteins extracted from gastric mucosal specimens were analysed by 2-dimensional electrophoresis, sequenced by MALDI-TOF and identified by Edman's degradation.


RESULTS
Twenty-one out of 41 patients had H. pylori infection of whom 17 had anti-CagA IgG antibodies. Several proteins were identified, of which Rho guanosine diphosphatase dissociation inhibitor alpha and heat shock protein 27 increased and glutathione transferase and antrum mucosa protein-18 decreased in H. pylori-positive in respect to H. pylori-negative patients. Interestingly, antrum mucosa protein-18, currently referred as gastrokine-1, showed two isoforms differing in the first N-terminal amino acid residue. Both gastrokine-1 isoforms were observed in the H. pylori-negative group whereas a lower expression or even absence of the gastrokine-1 basic isoform was found in a subgroup (7/21) of H. pylori-positive patients with moderate-severe gastritis.


CONCLUSIONS
Our study demonstrated the presence of gastrokine-1 isoforms of which the basic isoform was reduced in a subset of patients with H. pylori infection.
Nardone
G
G
Department of Clinical and Experimental Medicine, Gastroenterology, Federico II University of Naples, Via S. Pansini, 5 80131 Naples, Italy. nardone@unina.it
Rippa
E
E
Martin
G
G
Rocco
A
A
Siciliano
R A
RA
Fiengo
A
A
Cacace
G
G
Malorni
A
A
Budillon
G
G
Arcari
P
P
D000328:Adult; D015152:Blotting, Northern; D015153:Blotting, Western; D004415:Dyspepsia / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004720:Endonucleases / Q000096:biosynthesis*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D005786:Gene Expression Regulation; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000096:biosynthesis; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000096:biosynthesis; D020411:Oligonucleotide Array Sequence Analysis; D036361:Peptide Hormones; D020033:Protein Isoforms / Q000096:biosynthesis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors
C477000:GKN1 protein, human; D020730:Guanine Nucleotide Dissociation Inhibitors; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D036361:Peptide Hormones; D020033:Protein Isoforms; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D004720:Endonucleases