A new approach for the analysis of testicular cells using a laser scanning cytometer.
The laser scanning cytometer (LSC) is a new laboratory tool that offers increased sensitivity and specificity compared to traditional technology. By combining the properties of the advantages of flow cytometry and immunohistochemistry, LSC-based analysis allows the automated evaluation of testicular cells in general and meiosis in particular. Testicular cell smears with previous staining by propidium iodide were analyzed by LSC. The results were compared with those for flow cytometry. LSC is a new, applicable methodology for analyzing spermatogenesis schedule.
Kwon
Jungkee
J
Biosafety Research Institute, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk, Korea.
D000818:Animals; D002469:Cell Separation; D005434:Flow Cytometry / Q000379:methods; D047569:Laser Scanning Cytometry / Q000295:instrumentation / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013091:Spermatogenesis / Q000502:physiology; D013737:Testis / Q000166:cytology* / Q000502:physiology