Spectrophotometric and liquid chromatographic determination of dopamine from pharmaceutical preparations using acetyl acetone as derivatizing reagent.
Dopamine (DA) has been determined by spectrophotometry and by liquid chromatography as derivatives of acetylacetone (AA). The liquid chromatography of DA was carried out in the presence of hydroxyproline (HP), octopamine (OP) and tyramine (TY). The separation was obtained from Phenomenex C-18 column 5 pm (150 x 4.6 mm id) by isocratic elution with methanol: acetonitrile: water (45: 6: 49 v/v/v), UV detection was at 320 nm. Linear calibration curves were obtained for each with 2.5-40.0 microgml(-1) with detection limits in the range 1.8-80 ng injection(-1) (10 microl). The dopamine was determined in pharmaceutical preparations with coefficient of variation (CV) within 0.7-1.5%.
Khuhawar
M Y
MY
Dr. M.A. Qazi Institute of Chemistry, University of Sindh, Jamshoro, Pakistan.
Rajper
A D
AD
Rind
F M A
FM
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000032:analysis*; D010422:Pentanones; D004364:Pharmaceutical Preparations / Q000032:analysis; D013053:Spectrophotometry
D010422:Pentanones; D004364:Pharmaceutical Preparations; C008790:acetylacetone; D004298:Dopamine