Isolation of chloroplast proteins from Arabidopsis thaliana for proteome analysis.
This chapter describes a simple protocol for large-scale chloroplast purification for proteome analysis. The protocol has not been tested for protein import activity but is optimized for chloroplast proteome yield and purity and minimal protein degradation.
van Wijk
Klaas J
KJ
Department of Plant Biology, Cornell University, Ithaca, New York, USA.
Peltier
Jean-Benoit
JB
Giacomelli
Lisa
L
D017360:Arabidopsis / Q000737:chemistry*; D002458:Cell Fractionation; D002736:Chloroplasts / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D012996:Solutions
D010940:Plant Proteins; D012996:Solutions