Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay.
We evaluated a modified fluorescence in situ hybridization (FISH) assay for rapid (<1 h) identification of microorganisms in growth-positive blood cultures. The results were compared to those of the standard FISH technique and conventional culturing. The rapid identification of microorganisms with modified FISH can have important effects on clinical management of patients with bloodstream infections.
Peters
Remco P H
RP
Department of Medical Microbiology and Infection Control, VU University Medical Center, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands.
van Agtmael
Michiel A
MA
Simoons-Smit
Alberdina M
AM
Danner
Sven A
SA
Vandenbroucke-Grauls
Christina M J E
CM
Savelkoul
Paul H M
PH
D000368:Aged; D000369:Aged, 80 and over; D016470:Bacteremia / Q000175:diagnosis*; D001419:Bacteria / Q000302:isolation & purification*; D005260:Female; D016469:Fungemia / Q000175:diagnosis*; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male