Competitive immunoassay for clenbuterol using capillary electrophoresis with laser-induced fluorescence detection.
A competitive immunoassay for detecting clenbuterol in urine was established by capillary electrophoresis (CE) with laser-induced fluorescence (LIF). The clenbuterol was conjugated with bovine serum albumin (BSA), and then the derivative was labeled with fluorescein isothiocyanate (FITC) and competes for antibody with free clenbuterol in the sample. Under the optimal conditions, Free and bound FITC labeled clenbuterol was separated within 8 min with the relative standard deviation (R.S.D.) 0.72% for migration time and 2.8% for peak area. The detection limit reached 0.7 ng/ml.
Zhou
Jieyu
J
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O. Box 283, Shanghai 200237, PR China.
Xu
Xueshu
X
Wang
Yuxiao
Y
D000818:Animals; D002417:Cattle; D002976:Clenbuterol / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005453:Fluorescence; D007118:Immunoassay / Q000379:methods*; D017879:Isothiocyanates / Q000737:chemistry; D007834:Lasers; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry
D017879:Isothiocyanates; D012710:Serum Albumin, Bovine; C037152:isothiocyanic acid; D002976:Clenbuterol