High resolution tiling-path BAC array deletion mapping suggests commonly involved 3p21-p22 tumor suppressor genes in neuroblastoma and more frequent tumors.
The recurrent loss of 3p segments in neuroblastoma suggests the implication of 1 or more tumor suppressor genes but thus far few efforts have been made to pinpoint their detailed chromosomal position. To achieve this goal, array-based comparative genomic hybridization was performed on a panel of 23 neuroblastoma cell lines and 75 primary tumors using a tiling-path bacterial artificial chromosome array for chromosome 3p. A total of 45 chromosome 3 losses were detected, including whole chromosome losses, large terminal deletions and interstitial deletions. The latter, observed in cell lines as well as a number of distal deletions detected in primary tumors, allowed us to demarcate 3 minimal regions of loss of 3.6 Mb [3p21.31-p21.2, shortest regions of overlap (SRO)1], 1.4 Mb (3p22.3-3p22.2, SRO2) and 3.8 Mb (3p25.3-p25.1, SRO3) in size. The present data significantly extend previous findings and now firmly establish critical regions on 3p implicated in neuroblastoma. Interestingly, the 2 proximal regions coincide with previously defined SROs on 3p21.3 in more frequent tumors including lung and breast cancer. As such, similar tumor suppressor genes may play a critical role in development or progression of a variety of neoplasms, including neuroblastoma.
Hoebeeck
Jasmien
J
Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium.
Michels
Evi
E
Menten
Björn
B
Van Roy
Nadine
N
Eggert
Angelika
A
Schramm
Alexander
A
De Preter
Katleen
K
Yigit
Nurten
N
De Smet
Els
E
De Paepe
Anne
A
Laureys
Geneviève
G
Vandesompele
Jo
J
Speleman
Frank
F
D045744:Cell Line, Tumor; D002872:Chromosome Deletion; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D020451:Contig Mapping / Q000379:methods; D018450:Disease Progression; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor / Q000502:physiology; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D009693:Nucleic Acid Hybridization / Q000379:methods*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D025521:Tumor Suppressor Proteins