Immunohistochemical study on distribution of endocrine cells in gastrointestinal tract of flower fish (Pseudophoxinus antalyae).
OBJECTIVE
To detect distribution and relative frequency of endocrine cells in gastrointestinal tract of flower fish (Pseudophoxinus antalyae).


METHODS
The intestinal tract of flower fish was divided into four portions from proximal to distal; the enlarged area after oesophagus and anterior, middle and posterior intestine. Immunohistochemical method using the peroxidase anti-peroxidase complex was employed. All antisera between four portions of flower fish were compared using ANOVA.


RESULTS
Eleven types of gut endocrine cells were determined; they were immunoreactive for calcitonin gene related peptide, substance P, vasoactive intestinal peptide, bombesin, somatostatin-14, secretin, TrkA, TrkB, TrkC, neurotensin, neuropeptide Y, which were found in almost all portions of the gastrointestinal tract.


CONCLUSIONS
The regional distribution and relative frequency of immunoreactive cells in the flower fish, Pseudophoxinus antalyae, are essentially similar to those of other fish.
Cinar
Kenan
K
Department of Biology, Süleyman Demirel University, Isparta 32260, Turkey. kcinar@fef.sdu.edu.tr
Senol
Nurgül
N
Ozen
M Rüştü
MR
D000818:Animals; D001839:Bombesin / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D003530:Cyprinidae / Q000033:anatomy & histology* / Q000378:metabolism; D019858:Enteroendocrine Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D041981:Gastrointestinal Tract / Q000166:cytology* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D013004:Somatostatin / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D015740:Calcitonin Gene-Related Peptide; D001839:Bombesin