The hepatoprotective activity of kinsenoside from Anoectochilus formosanus.
Carbon tetrachloride (CCl(4)) causes chronic hepatitis, featuring an increase in hepatic hydroxyproline, spleen weight and serum GPT levels and a decrease in plasma albumin levels. Crude extracts of fresh whole plants of Anoectochilus formosanus showed inhibition of chronic hepatitis induced by CCl(4) in mice. Bioactivity-guided fractionation and spectroscopic analysis revealed that kinsenoside was the most active compound. In an in vitro study, the LD(50) values for H(2)O(2)-induced cytotoxicity in BALB/c normal liver cells were significantly higher after kinsenoside pretreatment than after vehicle alone, further confirming that kinsenoside shows significant antihepatotoxic activity.
Wu
Jin-Bin
JB
Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taiwan, ROC.
Lin
Wei-Lii
WL
Hsieh
Chang-Chi
CC
Ho
Hui-Ya
HY
Tsay
Hsin-Sheng
HS
Lin
Wen-Chuan
WC
D015107:4-Butyrolactone / Q000031:analogs & derivatives; D000818:Animals; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000175:diagnosis / Q000517:prevention & control*; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D009005:Monosaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D029595:Orchidaceae; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
C422363:3-glucopyranosyloxybutanolide; D009005:Monosaccharides; D010936:Plant Extracts; D020011:Protective Agents; D002251:Carbon Tetrachloride; D015107:4-Butyrolactone