Axonal transport of BDNF precursor in primary sensory neurons.
Recent studies have shown that the precursor of brain-derived neurotrophic factor (pro-BDNF) activates p75NTR with high affinity to induce apoptosis. Here we show that pro-BDNF is transported anterogradely and retrogradely in sensory neurons of adult rats. After a crush injury of sciatic nerves, dorsal roots or dorsal column in adult Sprague-Dawley rats, the immunoreactivity for pro-BDNF accumulated at both the proximal and distal segments. The accumulation reached a maximum at 24 h after injury. Western blot analysis also revealed pro-BDNF in sciatic nerve segments proximal and distal to the ligature and in the spinal cord. Biotinylated or Alexa-488-labelled pro-BDNF injected into sciatic nerve was internalized and transported both retrogradely and anterogradely within sensory neurons. These results demonstrate that pro-BDNF is anterogradely and retrogradely transported in sensory neurons, suggesting that endogenous pro-BDNF may be released and play important functions.
Wang
Hui
H
Department of Human Physiology and Centre for Neuroscience, Flinders University of South Australia, GPO Box 2100 Adelaide, SA 5001, Australia.
Wu
Linda Lin-Yan
LL
Song
Xing-Yun
XY
Luo
Xue-Gang
XG
Zhong
Jin-Hua
JH
Rush
Robert A
RA
Zhou
Xin-Fu
XF
D000818:Animals; D001370:Axonal Transport / Q000502:physiology*; D015153:Blotting, Western; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D051379:Mice; D009409:Nerve Crush; D009475:Neurons, Afferent / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries / Q000473:pathology; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology; D013126:Spinal Nerve Roots / Q000378:metabolism / Q000473:pathology
D019208:Brain-Derived Neurotrophic Factor