title: Bacterially expressed and optimized recombinant Phl p 1 is immunobiochemically equivalent to natural Phl p 1.
abstract: Recombinant production in bacteria of soluble and monomeric Phl p 1, a major allergen of Timothy grass pollen, has proved to be very problematic. In order to facilitate expression and purification of this allergen, a recombinant variant was designed with a single amino acid substitution. Several comparative analyses with natural counterparts using electrophoretic and HPLC separations, together with immunological assays, demonstrated high equivalence. This is the first description of an approach aiming at an improvement of a natural like recombinant allergen.
authors.affiliation: Allergopharma Joachim Ganzer KG, Hermann-Körner-Strasse 52, 21465 Reinbek, Germany. roland.suck@allergopharma
authors.forename: Roland
authors.initials: R
authors.lastname: Suck
authors.forename: Timo
authors.initials: T
authors.lastname: Kamionka
authors.forename: Brigitte
authors.initials: B
authors.lastname: Schäffer
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Wahl
authors.forename: Andreas
authors.initials: A
authors.lastname: Nandy
authors.forename: Bernhard
authors.initials: B
authors.lastname: Weber
authors.forename: Arnd
authors.initials: A
authors.lastname: Petersen
authors.forename: Ruth
authors.initials: R
authors.lastname: Birner-Grünberger
authors.forename: Siva Charan
authors.initials: SC
authors.lastname: D V
authors.forename: Walter
authors.initials: W
authors.lastname: Keller
authors.forename: Helmut
authors.initials: H
authors.lastname: Fiebig
authors.forename: Oliver
authors.initials: O
authors.lastname: Cromwell
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D000485:Allergens; D010940:Plant Proteins; D011994:Recombinant Proteins; C090396:PHLPI protein, Phleum pratense