title: Antibody reactivity to heat shock protein 70 and inner ear-specific proteins in patients with idiopathic sensorineural hearing loss.
abstract: Deafness is attributable to autoimmunity in a subset of adult patients with sensorineural hearing loss (SNHL) of unknown aetiology. To determine the roles of self-antigens in the pathogenesis of idiopathic SNHL, we analysed antibody responses to the inner ear-specific proteins, cochlin and beta-tectorin as well as the non-specific heat shock protein 70 (HSP70). Recombinant cochlin and beta-tectorin proteins were used in a qualitative Western blot assay for the detection of antigen-specific IgG antibodies in 58 patients with idiopathic SNHL and 28 healthy blood donors. In the same study cohort, we also used a Western blot assay to assess IgG antibody responses to the recombinant human HSP70. Of the 58 patient samples analysed, 19 tested positive to the HSP70, eight to cochlin and one to beta-tectorin, giving a prevalence of 33, 14 and 2%, respectively. Only one patient sample was reactive for HSP70, cochlin and beta-tectorin, seven of the remaining eight cochlin IgG antibody-positive samples were monospecific. Thus, cochlin-specific antibodies were observed predominantly in HSP70 IgG-negative patients demonstrating an additive value for testing this antibody response in patients with idiopathic SNHL.
authors.affiliation: Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA. anne.tebo@aruplab.com
authors.forename: A E
authors.initials: AE
authors.lastname: Tebo
authors.forename: P
authors.initials: P
authors.lastname: Szankasi
authors.forename: T A
authors.initials: TA
authors.lastname: Hillman
authors.forename: C M
authors.initials: CM
authors.lastname: Litwin
authors.forename: H R
authors.initials: HR
authors.lastname: Hill
mesh_terms: D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000096:biosynthesis*; D001324:Autoantigens / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology*; D007758:Ear, Inner / Q000276:immunology*; D016326:Extracellular Matrix Proteins / Q000276:immunology; D005260:Female; D058851:GPI-Linked Proteins; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D006319:Hearing Loss, Sensorineural / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D008297:Male; D008565:Membrane Proteins / Q000276:immunology; D008875:Middle Aged; D011506:Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; C110668:COCH protein, human; D016326:Extracellular Matrix Proteins; D058851:GPI-Linked Proteins; D018840:HSP70 Heat-Shock Proteins; D007074:Immunoglobulin G; D008565:Membrane Proteins; D011506:Proteins; D011994:Recombinant Proteins; C093123:TECTB protein, human