title: Study of hypothermia on cultured neuronal networks using multi-electrode arrays.
abstract: Efficient and safe use of hypothermia during various neuro-medical procedures requires sound understanding of low temperature effects on the neuronal network's activity. In this report, we introduce the use of cultivated dissociated neuronal networks on temperature controlled multi-electrode arrays (MEAs) as a simple methodology for studying the long-term effects of hypothermia. The networks exhibit spontaneous activity in the form of synchronized bursting events (SBEs), followed by long intervals of sporadic firing. Through the use of our correlation method, these SBEs can be clustered into sub-groups of similar spatio-temporal patterns. Application of hypothermia to the network resulted in a reduction in the SBE rate, the spike intensity and an increase in inter-neuronal correlations. Within 2h following the cessation of hypothermia, the cultured network returned to its initial spatio-temporal SBE structure. These results suggest that the network survived cold exposure and demonstrate the feasibility of long-term continuous neural network recording during hypothermic conditions.
authors.affiliation: School of Social Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel. LielRubinsky@yahoo.com
authors.forename: Liel
authors.initials: L
authors.lastname: Rubinsky
authors.forename: Nadav
authors.initials: N
authors.lastname: Raichman
authors.forename: Itay
authors.initials: I
authors.lastname: Baruchi
authors.forename: Mark
authors.initials: M
authors.lastname: Shein
authors.forename: Jacob
authors.initials: J
authors.lastname: Lavee
authors.forename: Hanan
authors.initials: H
authors.lastname: Frenk
authors.forename: Eshel
authors.initials: E
authors.lastname: Ben-Jacob
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001831:Body Temperature / Q000502:physiology*; D001921:Brain / Q000503:physiopathology*; D002478:Cells, Cultured; D003080:Cold Temperature / Q000009:adverse effects; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D007036:Hypothermia, Induced / Q000009:adverse effects*; D008839:Microelectrodes / Q000592:standards; D009415:Nerve Net / Q000503:physiopathology*; D009434:Neural Pathways / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology; D013997:Time Factors