[Impact of mobilization with rhG-CSF on the proliferation and cytotoxicity of donor's T cells].
The study was to understand the impact on the proliferation and cytotoxicity of donor's T cells during mobilization with rhG-CSF. The peripheral blood mononuclear cells (PBMNC) were collected from 15 donors before mobilization and on fifth day of mobilization with rhG-CSF. After the PBMNC were activated with 500 ng/ml of CD3 monoclonal antibody and 500 microg/ml of rhIL-2 for 96 hours, the activated T cells were collected for testing proliferation, cytotoxicity, Fas expression, perforin and Fas ligand (FasL) mRNA expression, the IFN-gamma concentration in the culture medium of the activated T cells was determined by radioimmunoassay. The results showed that the proliferation activity of T lymphocytes and the cytotoxicity of T cells activated with CD3 monoclonal antibody and rhIL-2 were reduced markedly after mobilization with rhG-CSF (P < 0.05). The Fas molecule expression in the activated T cells was very high both before and after mobilization with rhG-CSF (P > 0.10). The activated T cells expressed perforin mRNA and didn't express FasL mRNA both before and after mobilization with rhG-CSF. The concentration of IFN-gamma in the culture medium of the activated T cells decreased significantly after mobilization with rhG-CSF (P < 0.01). It is concluded that activity of proliferation and cytotoxicity of donor's T cells is impaired after mobilization with rhG-CSF.
Huang
Wen-Rong
WR
Department of Hematology, General Hospital of PLA, Beijing 100853, China.
Wang
Li-Sheng
LS
Gao
Chun-Ji
CJ
Lu
Zhuo-Zhuang
ZZ
Wang
Hua
H
Duan
Hai-Feng
HF
Da
Wan-Ming
WM
D000293:Adolescent; D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D053222:Fas Ligand Protein / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology; D019014:fas Receptor / Q000096:biosynthesis / Q000235:genetics
D053222:Fas Ligand Protein; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019014:fas Receptor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor