title: Biomolecular interaction analysis in drug discovery using surface plasmon resonance technology.
abstract: The review gives first an introduction into the basics of surface plasmon resonance technology. The physical principle is shortly discussed followed by a discussion of the experimental details to be considered when using this technology for biomolecular interaction analysis. Based on recent publications it is demonstrated that the technology has widespread applications in many fields of the drug discovery process. Protein/protein interactions can be monitored in real time when working with biopharmaceuticals as well as protein/small analyte interactions during hit finding, secondary screening, lead optimization and lead selection. Equilibrium binding constants, kinetic rate constants and thermodynamic parameters are obtained from such study that helps to understand the mechanism of the binding reactions. This information can be directly used to improve binding properties of a drug candidate.
authors.affiliation: F. Hoffmann-La Roche AG, Department of Pharma Research, CH-4070 Basel, Switzerland. walter.huber@roche.com
authors.forename: Walter
authors.initials: W
authors.lastname: Huber
authors.forename: Francis
authors.initials: F
authors.lastname: Mueller
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D015195:Drug Design; D007700:Kinetics; D008024:Ligands; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods; D013816:Thermodynamics
chemical_list: D000911:Antibodies, Monoclonal; D008024:Ligands; D004364:Pharmaceutical Preparations; D011506:Proteins