title: Inhibitory effect of chitooligosaccharides on matrix metalloproteinase-9 in human fibrosarcoma cells (HT1080).
abstract: Matrix metalloproteinase-9 (MMP-9) has gelatinase activity and plays an important role in cancer invasion and metastasis. Therefore, inhibition of specific types of MMPs including MMP-9 has become an attractive target for therapeutic intervention. The aim of this study was to investigate the effect of chitooligosaccharides (COS) on activity and expression of MMP-9 in HT1080 cells. The inhibitory effect of COS with different molecular masses was examined by gelatin zymography, reverse transcriptase-polymerase chain reaction (RT-PCR), gene reporter assay, and Western blot analysis. MMP-9 inhibition in the presence of COS was clearly observed in gelatin zymography. Specifically, 1- to 3-kDa COS (COS-I) exhibited the highest inhibitory effect on MMP-9 activity in HT1080 cells among tested molecular mass fractions. It was also found that COS-I was capable of inhibiting both gene and protein expression of MMP-9 (P<0.01). These results suggest that low molecular mass COS can be considered as a potent inhibitor of MMP-9.
authors.affiliation: Department of Chemistry, Pukyong National University, Busan, 608-737, Republic of Korea.
authors.forename: Quang
authors.initials: Q
authors.lastname: Van Ta
authors.forename: Moon-Moo
authors.initials: MM
authors.lastname: Kim
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
mesh_terms: D045744:Cell Line, Tumor; D002686:Chitin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D005354:Fibrosarcoma / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D014158:Transcription, Genetic
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D009844:Oligosaccharides; D002686:Chitin; D020780:Matrix Metalloproteinase 9