Respiratory nitrate ammonification by Shewanella oneidensis MR-1.
Anaerobic cultures of Shewanella oneidensis MR-1 grown with nitrate as the sole electron acceptor exhibited sequential reduction of nitrate to nitrite and then to ammonium. Little dinitrogen and nitrous oxide were detected, and no growth occurred on nitrous oxide. A mutant with the napA gene encoding periplasmic nitrate reductase deleted could not respire or assimilate nitrate and did not express nitrate reductase activity, confirming that the NapA enzyme is the sole nitrate reductase. Hence, S. oneidensis MR-1 conducts respiratory nitrate ammonification, also termed dissimilatory nitrate reduction to ammonium, but not respiratory denitrification.
Cruz-García
Claribel
C
Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824-1325, USA.
Murray
Alison E
AE
Klappenbach
Joel A
JA
Stewart
Valley
V
Tiedje
James M
JM
D000641:Ammonia / Q000378:metabolism*; D000693:Anaerobiosis; D016680:Genome, Bacterial; D009154:Mutation; D050901:Nitrate Reductase / Q000235:genetics / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D010084:Oxidation-Reduction; D020592:Shewanella / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D009566:Nitrates; D009573:Nitrites; D000641:Ammonia; D050901:Nitrate Reductase