Evaluation of gamma-interferon kinetics in HSV-1 infected mice in different days post infection (in vivo) and post re-stimulation (in vitro).
Gamma interferon (IFN-gamma) is among the most important immune factors for limiting of herpes simplex virus (HSV) infections. However, our knowledge about the kinetics of IFN-gamma production after HSV infection is limited. The present study examines the kinetics of IFN-gamma expression following secondary infection with HSV-1. Using semiquantitative RT-PCR assay, the expression of IFN-gamma in spleen lymphocytes was significantly detected on 14 days but not 7 days after intraperitoneal inoculation of HSV-1, while ELISA detected IFN-gamma on both days. At various hours after in vitro re-stimulation of spleen cells, RT-PCR showed a decreasing pattern of mRNA transcripts, whereas, ELISA assayed an increasing amount of secreted protein through the experiment. Despite the contrast results of ELISA and RT-PCR, regarding the short half-life of mRNA, the data are in correlation with each other and need to interpret.
Arefian
Ehsan
E
Department of Virology, School of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-111, Tehran, Iran.
Bamdad
Travat
T
Soleimanjahi
Hoorieh
H
Sabahi
Farzaneh
F
Akhoond
Mohamad Reza
MR
Parsania
Masoud
M
Ghaemi
Amir
A
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006561:Herpes Simplex / Q000276:immunology*; D018259:Herpesvirus 1, Human; D007371:Interferon-gamma / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D012333:RNA, Messenger; D007371:Interferon-gamma