[DNA-hydrolyzing IgG antibodies from the blood of patients with acquired immune deficiency syndrome].
DNAase activity of 110 samples of IgG from the blood of AIDS patients was analyzed. It was shown that the relative activity of preparations varies very much from patient to patient, but 96% preparations show detectable level of DNAase activity. Several rigid criteria were applied and it was shown that DNAase activity is an intrinsic property of antibodies from AIDS patients. It was shown that catalytic activity could posses not only intact IgG, but also separated light chains of polyclonal antibodies. The abzymes catalyze DNA hydrolysis effectively in a wild range of pH (5.0-9.5). K(M) and V(MaKC) values of antibody-dependent hydrolysis of DNA was estimated.
Odintsova
E S
ES
Kharitonova
M A
MA
Baranovskiĭ
A G
AG
Siziakina
L P
LP
Buneva
V N
VN
Nevinskiĭ
G A
GA
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D017151:Antibodies, Catalytic / Q000276:immunology / Q000378:metabolism; D001769:Blood / Q000276:immunology*; D002846:Chromatography, Affinity / Q000379:methods; D004247:DNA / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics
D017151:Antibodies, Catalytic; D007074:Immunoglobulin G; D004247:DNA