[Distribution of hepatitis B virus genotypes in Guizhou and analysis of clinical significance.].
BACKGROUND
To investigate the distribution of HBV genotypes in Guizhou and study the relationship between the genotype and the progression of liver disease.


METHODS
Totally 693 patients with chronic HBV infection, from 4 areas of Guizhou, including 292 asymptomatic HBV carriers (ASC), 276 cases with chronic hepatitis (CH), 76 liver cirrhosis (LC), 49 hepatocellular carcinoma (HCC) were examined. The HBV genotype was determined by restriction fragment length polymorphism analysis and the relationship between HBV genotype and the progression of liver disease was studied by multifactor analysis such as HBsAg positivity, HBV DNA load and ALT level.


RESULTS
Of the 693 patients, 6 (0.87%), 449 (64.79%), 233 (33.62%), and 5 (0.72%) belonged to genotype A, B, C and D, respectively. There was a statistically siganificant difference in the distribution of genotype B among Kaili (96.4%), Zunye (78.79%), Duyun (76.19%) and Guiyang (53.66%) areas (P less than 0.01). Genotype C was more prevalent in Guiyang than in other three areas (45.68% vs. 23.8%, 45.68% vs. 13.13%, 45.68% vs. 3.96%, P less than 0.01, respectively). There were also statistically significant difference in distribution of genotype B and C in various stages of liver disease (P less than 0.01). Genotype B showed a decreasing trend from ASC, CH, LC to HCC and genotype C was more frequent in patients with CH (45.92%) than in those with ASC (20.6%), LC (17.17%) and HCC (16.31%). The ALT levels and the mean ages were significantly higher and older in patients with genotype C than those in genotype B. The HBeAg positivity was significantly lower in genotype C than that in genotype B (48.07% vs 61.90%, P less than 0.01).


CONCLUSIONS
Genotype A, B, C and D exist in Guizhou. Genotype B predominates and genotype C was the second common. The geographic distribution of genotype B and C are different in some areas of Guizhou. Compared with genotype B, genotype C is associated with the development of more severe liver damage.
Ding
J J
JJ
Department of Infectious Diseases, Guiyang Medical College, Guiyang 550004, China. Corresponding author: DING Jing-juan, E-mail: DINGJj@vip.163.com.
Zhang
Q
Q
Peng
L
L
Liu
Y H
YH
Li
Z
Z
Liu
S D
SD
Hu
L
L
D002681:China; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D006515:Hepatitis B virus / Q000235:genetics; D019694:Hepatitis B, Chronic / Q000821:virology; D006801:Humans; D008113:Liver Neoplasms / Q000821:virology
D004279:DNA, Viral