title: Molecular characterization of miniature porcine RANTES and its chemotactic effect on human mononuclear cells.
abstract: To elucidate the potential role of porcine RANTES (Regulated upon Activation Normal T cells Expressed and Secreted) in xenograft rejection, we investigated its chemotactic activity for human mononuclear cells, as well as the effect of human cytokines on its expression in porcine endothelial cells. Porcine RANTES cDNA was successfully cloned from aortic endothelial cells of miniature pigs, and its protein expression was induced by transfection. Its deduced amino acid sequence was 83.5% identical to that of human RANTES. Porcine RANTES triggered transmigration of human mononuclear cells across the species barrier, and this chemotactic effect was suppressed by anti-RANTES neutralizing antibodies. The chemotactic effect of porcine RANTES was most prominent on human monocytes. Human tumor necrosis factor-alpha induced significant expression of porcine RANTES messenger RNA in endothelial cells; however both human interferon-gamma and interleukin-1beta failed. These results suggest that porcine RANTES can play an important role in xenotransplant rejection, through participating in the interaction between porcine endothelial cells and human monocytes.
authors.affiliation: Transplantation Research Institute, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Jaeseok
authors.initials: J
authors.lastname: Yang
authors.forename: Bumrae
authors.initials: B
authors.lastname: Cho
authors.forename: Inho
authors.initials: I
authors.lastname: Choi
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Kim
authors.forename: Sung Dae
authors.initials: SD
authors.lastname: Kim
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Koh
authors.forename: Han
authors.initials: H
authors.lastname: Ro
authors.forename: Kook Hwan
authors.initials: KH
authors.lastname: Oh
authors.forename: Junho
authors.initials: J
authors.lastname: Chung
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Curie
authors.initials: C
authors.lastname: Ahn
authors.forename: Suhnggwon
authors.initials: S
authors.lastname: Kim
authors.forename: Jung Sang
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018946:Chemokine CCL5 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000235:genetics; D016207:Cytokines / Q000494:pharmacology; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000187:drug effects / Q000276:immunology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D013552:Swine
chemical_list: D018946:Chemokine CCL5; D016207:Cytokines; D012333:RNA, Messenger