Broad-Complex, E74, and E75 early genes control DNA puff BhC4-1 expression in prepupal salivary glands.
The DNA puff BhC4-1 gene of the sciarid Bradysia hygida is induced in salivary glands prior to the pupal molt as a secondary response to the increase in ecdysone titers. Previous studies demonstrated that the BhC4-1 promoter is activated in transgenic Drosophila melanogaster salivary glands as a late response to the ecdysone peak that triggers metamorphosis, revealing that this aspect of BhC4-1 transcriptional regulation is conserved in the Drosophila background. To identify regulators of BhC4-1 expression, we utilized a candidate gene approach and tested the roles of the ecdysone-induced genes BR-C, E74, and E75. Our results reveal that the BR-C Z3 isoform is essential for BhC4-1-lacZ induction in prepupal salivary glands and constitute the first demonstration of the participation of early genes products on DNA puff genes regulation.
Basso
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LR
Departamento de Biologia Celular e Molecular e de Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
de C Neves
M
M
Monesi
N
N
Paçó-Larson
M L
ML
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics*; D004441:Ecdysterone / Q000378:metabolism / Q000494:pharmacology; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011987:Receptors, Steroid / Q000235:genetics*; D012469:Salivary Glands / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics*
C102117:Br protein, Drosophila; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C087173:E75 protein, insect; C060408:Eip74EF protein, Drosophila; D019476:Insect Proteins; D011987:Receptors, Steroid; D012471:Salivary Proteins and Peptides; D014157:Transcription Factors; C095605:puff C4 protein, Bradysia hygida; D004441:Ecdysterone