CDK1 and calcineurin regulate Maskin association with eIF4E and translational control of cell cycle progression.
Maskin regulates assembly of the eIF4F translation initiation complex on messenger RNAs that contain cytoplasmic polyadenylation elements (CPEs) in their 3' untranslated regions. Because Maskin and eIF4G contain similar peptide motifs that bind eIF4E, they compete for occupancy of this factor and consequently control translation. One mRNA that is regulated by Maskin encodes cyclin B1, whose translation oscillates with the early cell cycles of Xenopus laevis embryos. Here we show that Maskin phosphorylation-dephosphorylation also oscillates with the cell cycle and is controlled by the kinase CDK1 and the phosphatase calcineurin. These phosphorylation events control the Maskin-eIF4E interaction and, as a result, translation of cyclin B1 mRNA. Cell cycle progression requires this Maskin-mediated translational regulation.
Cao
Quiping
Q
Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation St., Worcester, Massachusetts 01605, USA.
Kim
Jong Heon
JH
Richter
Joel D
JD
D000595:Amino Acid Sequence; D000818:Animals; D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism*; D019703:Calcineurin / Q000378:metabolism*; D002453:Cell Cycle; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014981:Xenopus / Q000235:genetics / Q000378:metabolism; D029867:Xenopus Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D039561:Eukaryotic Initiation Factor-4E; C474872:TACC3 protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins; D016203:CDC2 Protein Kinase; D019703:Calcineurin