title: Identification of differentially expressed genes in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis.
abstract: OBJECTIVE
  To identify genes differentially expressed in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis.
  
  
  METHODS
  A subtracted cDNA library of mouse hepatocarcinoma cell line with low potential of lympho-genous metastasis Hca-P and its synogenetic cell line Hca-F with high metastatic potential was constructed by suppression subtracted hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed.
  
  
  RESULTS
  Fifteen differentially expressed cDNA fragments of Hca-P were obtained which revealed 8 known genes, 4 expressed sequence tags (ESTs) and 3 cDNAs showed no homology.
  
  
  CONCLUSIONS
  Tumor metastasis is an incident involving multiple genes. SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.
authors.affiliation: Department of Oncology, The 1st Affiliated Dalian Medical University, Dalian 116027, Liaoning Province, China.
authors.forename: Xiao-Nan
authors.initials: XN
authors.lastname: Cui
authors.forename: Jian-Wu
authors.initials: JW
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Hou
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Ban
mesh_terms: D000818:Animals; D001201:Ascites / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006824:Hybridization, Genetic; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000235:genetics; D013489:Suppression, Genetic
chemical_list: D012333:RNA, Messenger; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases