title: Osteogenic potential using a malleable, biodegradable composite added traditional Chinese medicine: in vitro and in vivo evaluations.
abstract: The purpose of this investigation was to prepare and evaluate the feasibility and biocompatibility of a new composite as a large defect bone substitute. The new GTGG was mainly composed of tricalcium phosphate ceramic particles and glutaraldehyde crosslinked gelatin in which Gui-Lu-Jiao was added (a mixture of Cervi Colla Cornus and Colla Plastri Testudinis). In the in vitro study, rat's calvaria osteoblasts were used to study bone characteristics upon exposure to different concentrations of the Gui-Lu-Jiao solution. In the in vivo study, GTGG composites were implanted into the defects of calvarial bones in mature New Zealand rabbits to test their osteogenerative characteristics. As a result, we found that Gui-Lu-Jiao added to the culture could promote the proliferation of osteoblasts. In addition, GTGG could induce a large amount of new bone growth in the rabbit's calvarial bone defect. Therefore, the GTGG composite might be a potential bone substitute.
authors.affiliation: Institute of Biomedical Engineering and Material Science, Central Taiwan University of Science and Technology, Taichung, Taiwan, ROC.
authors.forename: Chun-Hsu
authors.initials: CH
authors.lastname: Yao
authors.forename: Bai-Shuan
authors.initials: BS
authors.lastname: Liu
authors.forename: Chau-Guey
authors.initials: CG
authors.lastname: Liu
authors.forename: Yueh-Sheng
authors.initials: YS
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005240:Feasibility Studies; D020007:Implants, Experimental; D008516:Medicine, Chinese Traditional; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D012886:Skull / Q000473:pathology / Q000503:physiopathology / Q000601:surgery
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D000469:Alkaline Phosphatase