title: Rapid molecular strategy for filovirus detection and characterization.
abstract: Filoviruses have the capacity to cause lethal outbreaks of hemorrhagic fever in primates. Here we present a simple consensus reverse transcription-PCR method for filovirus recognition and characterization and demonstrate its utility with all known filovirus strains. Phylogenetic assignment is achieved by automated web-based sequence analysis of amplification products.
authors.affiliation: Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, New York, NY 10032, USA.
authors.forename: Junhui
authors.initials: J
authors.lastname: Zhai
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Towner
authors.forename: Omar
authors.initials: O
authors.lastname: Jabado
authors.forename: Vishal
authors.initials: V
authors.lastname: Kapoor
authors.forename: Marietjie
authors.initials: M
authors.lastname: Venter
authors.forename: Allen
authors.initials: A
authors.lastname: Grolla
authors.forename: Thomas
authors.initials: T
authors.lastname: Briese
authors.forename: Janusz
authors.initials: J
authors.lastname: Paweska
authors.forename: Robert
authors.initials: R
authors.lastname: Swanepoel
authors.forename: Heinz
authors.initials: H
authors.lastname: Feldmann
authors.forename: Stuart T
authors.initials: ST
authors.lastname: Nichol
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
mesh_terms: D000818:Animals; D029043:Ebolavirus / Q000235:genetics / Q000302:isolation & purification; D016563:Filoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D018702:Filoviridae Infections / Q000175:diagnosis* / Q000821:virology*; D019142:Hemorrhagic Fever, Ebola; D006801:Humans; D008379:Marburg Virus Disease; D029024:Marburgvirus / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors