title: [Accelerated somatic cell senescence and changes in p16INK4a expression after exogenous DNA transfection].
abstract: During the transfection of mouse embryonic fibroblasts (MEF), we found these cells became senescent, appearing enlarged with hollow cytoplasm and multinucleated. The telomere lengths of these senescent MEFs were significantly shorter than primary untransfected MEFs. In senescent MEF cells, DNA methylation of p16INK4a 5'-regulatory region showed gradual reduction as cells aged. RT-PCR and Northern blot demonstrated that the expression of p16INK4a in transfected senescent cells was 12-16 times more than primary cells. The senescent transfected MEF cells as donors of nuclei could support the early development of cloned embryos after nuclear transfer.
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Science, Beijing 100071, China. shenwei427@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Wu
authors.forename: Yan-Rong
authors.initials: YR
authors.lastname: Zhou
authors.forename: Qing-Jie
authors.initials: QJ
authors.lastname: Pan
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Ji-Xian
authors.initials: JX
authors.lastname: Deng
mesh_terms: D000818:Animals; D016922:Cellular Senescence / Q000235:genetics*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D019175:DNA Methylation; D005347:Fibroblasts / Q000166:cytology*; D015870:Gene Expression; D051379:Mice; D053652:Nuclear Transfer Techniques; D016615:Telomere / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D019076:Transgenes
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D004247:DNA