title: Expression profiling of heat stress effects on mice fed ergot alkaloids.
abstract: Fescue toxicosis affects wild and domestic animals consuming ergot alkaloids contained in tall fescue forage infected with the endophytic fungus, Neotyphodium coenophialum. When animals are consuming infected fescue (E+) forage during periods of elevated ambient temperatures (summer), a range of phenotypic disorders collectively called summer slump is observed. It is characterized by hyperthermia, with an accompanying decrease in feed intake, growth, milk yield, and reproductive fitness. Laboratory mice also exhibit symptoms of fescue toxicosis at thermoneutral (TN) temperature, as indicated by reduced growth rate and reproductive fitness. Our goal was to characterize the differences in gene expression in liver of mice exposed to summer-type heat stress (HS) and E+ when compared to mice fed E+ at TN temperature. Mice were fed E+ diet under HS (34 +/- 1 degrees C; n = 13; E+HS) or TN conditions (24 +/- 1 degrees C; n = 14; E+TN) for a period of 2 weeks between 47 and 60 days of age. Genes differentially expressed between E+HS versus E+TN were identified using DNA microarrays. Forty-one genes were differentially expressed between treatment groups. Expressions of eight genes were measured using quantitative real-time PCR. Genes coding for phase I detoxification enzymes were upregulated in E+HS mouse liver. This detoxification pathway is known to produce reactive oxidative species. We observed an upregulation of genes involved in the protection against reactive oxidative species. Key genes involved in de novo lipogenesis and lipid transport were also upregulated. Finally, genes involved in DNA damage control and unfolded protein responses were downregulated.
authors.affiliation: Division of Animal Sciences, University of Missouri-Columbia, Columbia, MO 65211, USA.
authors.forename: Sachin
authors.initials: S
authors.lastname: Bhusari
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Liu
authors.forename: Leonard B
authors.initials: LB
authors.lastname: Hearne
authors.forename: Donald E
authors.initials: DE
authors.lastname: Spiers
authors.forename: William R
authors.initials: WR
authors.lastname: Lamberson
authors.forename: Eric
authors.initials: E
authors.lastname: Antoniou
mesh_terms: D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D004032:Diet; D004876:Ergot Alkaloids / Q000032:analysis / Q000633:toxicity*; D031751:Festuca / Q000737:chemistry / Q000382:microbiology; D020869:Gene Expression Profiling; D018882:Heat Stress Disorders / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D015651:Mycotoxicosis / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry / Q000382:microbiology; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D004876:Ergot Alkaloids