Distribution of Tankyrase-1 mRNA expression in colon cancer and its prospective correlation with progression stage.
We tested Tankyrase-1 mRNA expression in colon cancer patients to evaluate the prognostic role of this parameter by real-time RT-PCR in a retrospective group of 82 unselected patients with colon cancer. Paired cancer and corresponding not affected tissues were used. Laser-assisted microdissection was used to measure Tankyrase-1 mRNA in homogeneous cancer cell populations and in normal colon epithelium of the same patients. Tankyrase-1 mRNA in colon cancers, as a mean, was significantly higher than in paired not affected tissues (p<0.0001), but its level correlates inversely with a cancer progression stage. Survival analysis indicated that lower Tankyrase-1 mRNA expression in colon cancers was significantly associated to reduced patient survival (p=0.019) and disease-free interval (p=0.035), confirmed also in a multi-variate analysis.
Gelmini
Stefania
S
Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence 50139, Italy.
Poggesi
Melissa
M
Pinzani
Pamela
P
Mannurita
Sara Ciullini
SC
Cianchi
Fabio
F
Valanzano
Rosa
R
Orlando
Claudio
C
D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007834:Lasers; D008297:Male; D042282:Microdissection; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis; D015996:Survival Rate; D038501:Tankyrases / Q000096:biosynthesis*
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D038501:Tankyrases; C418109:TNKS protein, human