title: A 2a/1b full-length p7 inter-genotypic chimeric genome of hepatitis C virus is infectious in vitro.
abstract: The p7 protein of hepatitis C virus (HCV) functions as an ion channel in planar lipid bilayers, and its function is vital for the virus life cycle. In this study, we replaced either the entire or partial p7 of genotype 2a (strain JFH1), an HCV strain that replicates and produces virus progeny in vitro, with the corresponding regions of the p7 protein from genotype 1b (Australian isolate, HCV-A). Compared to wild type, the chimeric viruses reached their peak of infectivity with a delay but they produced a comparable titer to the wild type virus and the progeny viruses were able to infect naive permissive cells. Amantadine treatment of wild type and chimeric viruses reduced the virus titers by about 50% and 45%, respectively. Therefore, in this study, for the first time, we demonstrated that genotype 2a (JFH1 strain) genome encoding a full-length genotype 1b p7 gene produces infectious particles in vitro. These chimeric viruses are valuable instruments for comparative studies of the p7 proteins.
authors.affiliation: The Macfarlane Burnet Institute, GPO Box 2284, Melbourne, VIC 3001, Australia. haqshenas@burnet.edu.au
authors.forename: G
authors.initials: G
authors.lastname: Haqshenas
authors.forename: X
authors.initials: X
authors.lastname: Dong
authors.forename: G
authors.initials: G
authors.lastname: Ewart
authors.forename: S
authors.initials: S
authors.lastname: Bowden
authors.forename: E J
authors.initials: EJ
authors.lastname: Gowans
mesh_terms: D000547:Amantadine / Q000494:pharmacology; D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D016679:Genome, Viral; D015658:HIV Infections / Q000821:virology*; D016174:Hepacivirus / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D016865:Reassortant Viruses / Q000187:drug effects / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014764:Viral Proteins / Q000502:physiology*; D014774:Virulence; D014779:Virus Replication / Q000187:drug effects; D000086082:Viroporin Proteins
chemical_list: D000547:Amantadine; D000998:Antiviral Agents; D014764:Viral Proteins; C088369:p7 protein, Hepatitis C virus; D000086082:Viroporin Proteins