title: Improvements in the analysis of decabromodiphenyl ether using on-column injection and electron-capture detection.
abstract: During the development of a method to determine polybrominated diphenyl ethers (PBDEs) using GC/MS-MS equipped with a programmed temperature vaporizer (PTV) injector, a dramatic decrease in sensitivity to decabromodiphenyl ether (BDE-209) occurred and lack of sensitivity and repeatability was observed. An alternative method using GC/ECD equipped with an on-column injector was evaluated for this congener optimizing pre-column and column length. A coated retention gap (50 cm of length, 0.53 mm I.D., 0.15 microm d(f)) connected to a short capillary column (7 m of length, 0.32 mm I.D., 0.25 microm d(f)) showed the better sensitivity and repeatability. Finally, a double system based on the programmed temperature vaporizer injector/MS detector (for tri- to hepta-BDEs) and on-column injector/electron-capture detector (for BDE-209) was evaluated using two candidate certified materials (fish muscle tissue and river sediment).
authors.affiliation: University of Milan, Department of Biology, 20133 Milan, Italy. Andea.Binelli@unimi.it
authors.forename: Andrea
authors.initials: A
authors.lastname: Binelli
authors.forename: Claudio
authors.initials: C
authors.lastname: Roscioli
authors.forename: Licia
authors.initials: L
authors.lastname: Guzzella
mesh_terms: D000818:Animals; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D005432:Flounder; D019015:Geologic Sediments / Q000737:chemistry; D055768:Halogenated Diphenyl Ethers; D009132:Muscles / Q000737:chemistry; D010647:Phenyl Ethers / Q000032:analysis*; D011075:Polybrominated Biphenyls / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D055768:Halogenated Diphenyl Ethers; D010647:Phenyl Ethers; D011075:Polybrominated Biphenyls; C010902:decabromobiphenyl ether