title: Transcriptional control of Notch signaling by a HOX and a PBX/EXD protein during vulval development in C. elegans.
abstract: The Notch signaling pathway controls growth, differentiation and patterning in divergent animal phyla; in humans, defective Notch signaling has been implicated in cancer, stroke and neurodegenerative disorders. Despite its developmental and medical significance, little is known about the factors that render cells to become competent for Notch signaling. Here we show that during vulval development in the nematode Caenorhabditis elegans the HOX protein LIN-39 and its EXD/PBX-like cofactor CEH-20 are required for LIN-12/Notch-mediated lateral signaling that specifies the 2 degrees vulval cell fate. Inactivation of either lin-39 or ceh-20 resulted in the misspecification of 2 degrees vulval cells and suppressed the multivulva phenotype of lin-12(n137) gain-of-function mutant animals. Furthermore, both LIN-39 and CEH-20 are required for the expression of basal levels of the genes encoding the LIN-12/Notch receptor and one of its ligands in the vulval precursor cells, LAG-2/Delta/Serrate, rendering them competent for the subsequent lin-12/Notch induction events. Our results suggest that the transcription factors LIN-39 and CEH-20, which function at the bottom of the RTK/Ras and Wnt pathways in vulval induction, serve as major integration sites in coordinating and transmitting signals to the LIN-12/Notch cascade to regulate vulval cell fates.
authors.affiliation: Department of Genetics, Eötvös Loránd University, Budapest, H-1117, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Takács-Vellai
authors.forename: Tibor
authors.initials: T
authors.lastname: Vellai
authors.forename: Estella B
authors.initials: EB
authors.lastname: Chen
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Frédéric
authors.initials: F
authors.lastname: Guerry
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Stern
authors.forename: Fritz
authors.initials: F
authors.lastname: Müller
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000502:physiology*; D005260:Female; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D007814:Larva; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology; D051880:Receptors, Notch / Q000502:physiology*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014844:Vulva / Q000254:growth & development / Q000502:physiology
chemical_list: D029742:Caenorhabditis elegans Proteins; D018398:Homeodomain Proteins; C082460:Lin-12 protein, C elegans; D008565:Membrane Proteins; D051880:Receptors, Notch; D014157:Transcription Factors; C418523:ceh-20 protein, C elegans; C086488:lag-2 protein, C elegans; C108673:lin-39 protein, C elegans