title: The TIGR Plant Transcript Assemblies database.
abstract: The TIGR Plant Transcript Assemblies (TA) database (http://plantta.tigr.org) uses expressed sequences collected from the NCBI GenBank Nucleotide database for the construction of transcript assemblies. The sequences collected include expressed sequence tags (ESTs) and full-length and partial cDNAs, but exclude computationally predicted gene sequences. The TA database includes all plant species for which more than 1000 EST or cDNA sequences are publicly available. The EST and cDNA sequences are first clustered based on an all-versus-all pairwise sequence comparison, followed by the generation of consensus sequences (TAs) from individual clusters. The clustering and assembly procedures use the TGICL tool, Megablast and the CAP3 assembler. The UniProt Reference Clusters (UniRef100) protein database is used as the reference database for the functional annotation of the assemblies. The transcription orientation of each TA is determined based on the orientation of the alignment with the best protein hit. The TA sequences and annotation are available via web interfaces and FTP downloads. Assemblies can be retrieved by a text-based keyword search or a sequence-based BLAST search. The current version of the TA database is Release 2 (July 17, 2006) and includes a total of 215 plant species.
authors.affiliation: The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Childs
authors.forename: John P
authors.initials: JP
authors.lastname: Hamilton
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Eugene
authors.initials: E
authors.lastname: Ly
authors.forename: Foo
authors.initials: F
authors.lastname: Cheung
authors.forename: Hank
authors.initials: H
authors.lastname: Wu
authors.forename: Pablo D
authors.initials: PD
authors.lastname: Rabinowicz
authors.forename: Chris D
authors.initials: CD
authors.lastname: Town
authors.forename: C Robin
authors.initials: CR
authors.lastname: Buell
authors.forename: Agnes P
authors.initials: AP
authors.lastname: Chan
mesh_terms: D018076:DNA, Complementary / Q000737:chemistry; D030561:Databases, Nucleic Acid; D030562:Databases, Protein; D020224:Expressed Sequence Tags / Q000737:chemistry; D020407:Internet; D010940:Plant Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry*; D018749:RNA, Plant / Q000737:chemistry*; D014584:User-Computer Interface
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant