Quantitative analysis of representative proteome components and clustering of Helicobacter pylori clinical strains.
BACKGROUND
Several Helicobacter pylori proteins have been reported to be associated with severe symptoms of gastric disease. However, expression levels of most of these disease-associated proteins require further evaluation in order to clarify their relationships with gastric disease patterns. Representative proteome components of 71 clinical isolates of H. pylori were analyzed quantitatively to determine whether the protein expression levels were associated with gastric diseases and to cluster clinical isolates.


METHODS
After two-dimensional electrophoresis (2-DE) of H. pylori isolates, spot intensities were analyzed using pdquest 2-D Gel Analysis Software. The intensities of 10 representative protein spots, identified by peptide fingerprinting using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) or peptide sequencing using quadrupole TOF MS, were subjected to the nonparametric Mann-Whitney test and hierarchical agglomerative cluster analysis. The relationship between clusters and gastric diseases was analyzed by the chi-squared test.


RESULTS
Although the spot intensities of the 10 representative proteins were highly variable within each gastric disease group, the expression levels of CagA, UreB, GroEL, EF-Tu, EF-P, TagD, and FldA showed some significant differences among the gastric disease patterns. On the basis of the 10 target protein intensities, hierarchical agglomerative cluster analysis generated a dendrogram with clusters indicative of chronic gastritis/gastric cancers and gastric/duodenal ulcers.


CONCLUSIONS
These results indicated that quantitative analysis of proteome components is a feasible method for examining disease-associated proteins and clustering clinical strains of H. pylori.
Park
Jeong-Won
JW
Department of Microbiology, Gyeongsang National University College of Medicine, Jinju, Gyeong-Nam, Korea.
Song
Jae-Young
JY
Lee
Seung-Gyu
SG
Jun
Jin-Su
JS
Park
Jeong-Uck
JU
Chung
Mi-Ja
MJ
Ju
Jung-Soo
JS
Nizamutdinov
Damir
D
Chang
Myung-Woong
MW
Youn
Hee-Shang
HS
Kang
Hyung-Lyun
HL
Baik
Seung-Chul
SC
Lee
Woo-Kon
WK
Cho
Myung-Je
MJ
Rhee
Kwang-Ho
KH
D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D001706:Biopsy; D002908:Chronic Disease; D004381:Duodenal Ulcer / Q000150:complications / Q000382:microbiology / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005420:Flavoproteins / Q000032:analysis / Q000378:metabolism; D005756:Gastritis / Q000150:complications / Q000382:microbiology / Q000473:pathology; D006360:Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism; D016481:Helicobacter Infections / Q000150:complications / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010444:Peptide Elongation Factor Tu / Q000032:analysis / Q000378:metabolism; D010445:Peptide Elongation Factors / Q000032:analysis / Q000378:metabolism; D010544:Peroxidases / Q000032:analysis / Q000378:metabolism; D012984:Software; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000150:complications / Q000382:microbiology / Q000473:pathology; D013276:Stomach Ulcer / Q000150:complications / Q000382:microbiology / Q000473:pathology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D005420:Flavoproteins; C120194:FldA protein, Bacteria; D006360:Heat-Shock Proteins; D010445:Peptide Elongation Factors; C080866:cagA protein, Helicobacter pylori; C020746:factor EF-P; D010544:Peroxidases; D010444:Peptide Elongation Factor Tu