title: Adipose tissues differentiated by adipose-derived stem cells harvested from transgenic mice.
abstract: OBJECTIVE
  To induce adipocyte differentiation in vitro by adipose-derived stromal cells (ASCs) harvested from transgenic mice with green fluorescent protein (GFP)and assess the possibility of constructing adipose tissues via attachment of ASCs to type I collagen scaffolds.
  
  
  METHODS
  Inguinal fat pads from GFP transgenic mice were digested by enzymes for isolation of ASCs (primary culture). After expansion to three passages of ASCs, the cells were incubated in an adipogenic medium for two weeks, and the adipocyte differentiation by ASCs in vitro was assessed by morphological observation and Oil Red O staining. Then they were attached to collagen scaffolds and co-cultured for 12 hours, followed by hypodermic implantation to the dorsal skin of nude mice for 2 months. The newly-formed tissues were detected by HE staining.
  
  
  RESULTS
  The cultured primary stem cells were fibroblast-like and showed active proliferation. After being incubated in an adipocyte differentiation medium, the lipid droplets in the cytoplasm accumulated gradually and finally developed into mature adipocytes, which showed positive in Oil Red O staining. A 0.5-cm3 new tissue clot was found under the dorsal skin of the nude mice and it was confirmed as mature adipose tissues by fluorescent observation and HE staining.
  
  
  CONCLUSIONS
  ASCs can successfully differentiate adipose tissues into mature adipocytes, which exhibit an adipocyte-like morphology and express as intracytoplasmic lipid droplets. It is an efficient model of adipose tissues engineered with ASCs and type I collagen scaffolds.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nanfang Medical University, Guangzhou 510515, China. lufengmail2005@hotmail.com
authors.forename: Feng
authors.initials: F
authors.lastname: Lu
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Gao
authors.forename: Rei
authors.initials: R
authors.lastname: Ogawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizuro
authors.forename: Hiki
authors.initials: H
authors.lastname: Hykusoku
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*