CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis.
CD45 is a membrane tyrosine phosphatase that modulates the function of the hematopoietic cells. In vitro, agonist antibodies to CD45RO or CD45RB isoforms have been shown to suppress microglial activation, but whether microglia in vivo express these isoforms in HIV encephalitis (HIVE) is unknown. Brain sections from control and HIVE were immunostained for CD45 isoforms using exon-specific antibodies (RA, RB, RC and RO). RA and RC were limited to rare lymphocytes, while RB expression was robust in microglia and inflammatory cells. RO was low in control microglia, but increased in HIVE. RO was also localized to macrophages and CD8+ T cells. Targeting CD45 in vivo with isoform-specific antibodies remains a therapeutic option for neuroinflammatory diseases.
Cosenza-Nashat
Melissa A
MA
Department of Science, BMCC, City University of New York, New York, NY, USA.
Kim
Mee-Ohk
MO
Zhao
Meng-Liang
ML
Suh
Hyeon-Sook
HS
Lee
Sunhee C
SC
D015526:AIDS Dementia Complex / Q000209:etiology / Q000276:immunology / Q000378:metabolism*; D001921:Brain / Q000276:immunology / Q000473:pathology*; D015497:HIV-1 / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D017628:Microglia / Q000378:metabolism*; D008875:Middle Aged; D020033:Protein Isoforms / Q000096:biosynthesis
D020033:Protein Isoforms; D017493:Leukocyte Common Antigens