title: Platelet-rich plasma preparation using three devices: implications for platelet activation and platelet growth factor release.
abstract: BACKGROUND
  In this study, three commercial systems for the preparation of platelet-rich plasma (PRP) were compared and platelet growth factors release was measured.
  
  
  METHODS
  Ten healthy volunteers donated whole blood that was fractionated by a blood cell separator, and a table-top centrifuge to prepare PRP. Furthermore, an autologous growth factor filter was used to concentrate PRP fractionated by the blood cell separator. PRP was subsequently activated with autologously produced thrombin to degranulate the platelets to measure platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-beta (TGF-beta), insulin-like growth factor-1 (IGF-1), and vascular endothelial growth factor (VEGF).
  
  
  RESULTS
  PRP contained significantly higher platelet counts compared with baseline values (p < 0.001). PDGF-AB concentrations were increased more than 18-fold in the platelet gel supernatant when the cell-separator and GPS were used, whereas only a 3-fold increase was seen with the AGF.
  
  
  CONCLUSIONS
  The three PRP devices enable the preparation of PRP for the release of high concentrations of platelet growth factor, but showed different harvesting capacities for the collection of concentrated platelets. The administration of thrombin for PRP activation resulted in the release of high concentrations of PDGF-AB and TGF-beta but only when PRP had not been activated during the preparation process in vitro.
authors.affiliation: Department of Extra Corporeal Blood Management, Catharina Hospital Eindhoven, Eindhoven, The Netherlands.
authors.forename: Peter A M
authors.initials: PA
authors.lastname: Everts
authors.forename: Christine
authors.initials: C
authors.lastname: Brown Mahoney
authors.forename: Johannes J M L
authors.initials: JJ
authors.lastname: Hoffmann
authors.forename: Jacques P A M
authors.initials: JP
authors.lastname: Schönberger
authors.forename: Henk A M
authors.initials: HA
authors.lastname: Box
authors.forename: André
authors.initials: A
authors.lastname: van Zundert
authors.forename: Johannes T A
authors.initials: JT
authors.lastname: Knape
mesh_terms: D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000187:drug effects; D010956:Plasmapheresis / Q000295:instrumentation* / Q000379:methods; D015539:Platelet Activation / Q000187:drug effects; D010976:Platelet Count; D017713:Platelet Transfusion / Q000295:instrumentation* / Q000379:methods; D010982:Platelet-Derived Growth Factor / Q000187:drug effects; D053657:Platelet-Rich Plasma; D013917:Thrombin / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000187:drug effects; D042461:Vascular Endothelial Growth Factor A / Q000187:drug effects
chemical_list: D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D007334:Insulin-Like Growth Factor I; D013917:Thrombin