title: Mycotoxin identification in moldy dwellings.
abstract: The objective of this study was to assess the level of macrocyclic trichothecenes (MCT), one of the most potent mycotoxins, on wall surfaces, floor dust and air samples from moldy dwellings. The study was based on an index group comprising 15 flooded dwellings contaminated by Stachybotrys chartarum or Chaetomium and a control group comprising nine dwellings without molds on visual inspection and mold sampling. Three samples were collected from each dwelling: a sample from the moldy wall, using a swab, a floor dust sample on a 0.5 m(2) surface with a cloth and an air sample using a pump comprising a 1 microm Teflon filter. The MCT level was measured using a monoclonal, antibody-based ELISA test. Compared with measurements performed in index dwellings, higher MCT values were measured on floor dust samples from moldy dwellings (P = 0.02). Samples from wall surfaces demonstrated nearly significant differences (P = 0.06). No significant differences were observed for air samples (P = 0.15), but some samples showed increased MCT levels. Significant correlations were observed between the levels measured on wall surfaces, floor dust and air samples (P = 0.02 to 0.05). In contrast, no correlation was observed between moldy surface and MCT levels. In conclusion, this paper reports for the first time direct evidence for the presence of MCT in moldy dwellings.
authors.affiliation: Maison de l'Allergie et de l'Environnement, Marseille, France.
authors.forename: Carmel
authors.initials: C
authors.lastname: Charpin-Kadouch
authors.forename: Gilles
authors.initials: G
authors.lastname: Maurel
authors.forename: Rachel
authors.initials: R
authors.lastname: Felipo
authors.forename: J
authors.initials: J
authors.lastname: Queralt
authors.forename: Myriam
authors.initials: M
authors.lastname: Ramadour
authors.forename: Henri
authors.initials: H
authors.lastname: Dumon
authors.forename: Max
authors.initials: M
authors.lastname: Garans
authors.forename: Alain
authors.initials: A
authors.lastname: Botta
authors.forename: Denis
authors.initials: D
authors.lastname: Charpin
mesh_terms: D000391:Air Microbiology; D016902:Air Pollution, Indoor; D004797:Enzyme-Linked Immunosorbent Assay; D006798:Housing; D009183:Mycotoxins / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis*
chemical_list: D009183:Mycotoxins; D014255:Trichothecenes