Lhca5 interaction with plant photosystem I.
In the outer antenna (LHCI) of higher plant photosystem I (PSI) four abundantly expressed light-harvesting protein of photosystem I (Lhca)-type proteins are organized in two heterodimeric domains (Lhca1/Lhca4 and Lhca2/Lhca3). Our cross-linking studies on PSI-LHCI preparations from wildtype Arabidopsis and pea plants indicate an exclusive interaction of the rarely expressed Lhca5 light-harvesting protein with LHCI in the Lhca2/Lhca3-site. In PSI particles with an altered LHCI composition Lhca5 assembles in the Lhca1/Lhca4 site, partly as a homodimer. This flexibility indicates a binding-competitive model for the LHCI assembly in plants regulated by molecular interactions of the Lhca proteins with the PSI core.
Lucinski
Robert
R
Adam Mickiewicz University, Department of Plant Physiology, Institute of Experimental Biology, Al. Niepodleglosci 14, 61-713, Poznan, Poland.
Schmid
Volkmar H R
VH
Jansson
Stefan
S
Klimmek
Frank
F
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D018532:Pisum sativum / Q000235:genetics / Q000378:metabolism*; D045331:Photosystem I Protein Complex / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; C493777:Lhca5 protein, Arabidopsis; D045342:Light-Harvesting Protein Complexes; D045331:Photosystem I Protein Complex