title: Isolation and characterization of the first American bottlenose dolphin papillomavirus: Tursiops truncatus papillomavirus type 2.
abstract: A novel papillomavirus (PV) was isolated from a genital condyloma of a free-ranging bottlenose dolphin inhabiting the coastal waters of Charleston Harbor, SC, USA: Tursiops truncatus papillomavirus type 2 (TtPV2). This novel virus represents the first isolated North American cetacean PV and the first American bottlenose dolphin PV. After the viral genome was cloned, sequenced and characterized genetically, phylogenetic analyses revealed that TtPV2 is most similar to the only published cetacean PV isolated and characterized thus far, Phocoena spinipinnis PV type 1 (PsPV1). A striking feature of the genome of TtPV2, as well as that of PsPV1, is the lack of an E7 open reading frame, which typically encodes one of the oncogenic proteins believed to be responsible for malignant transformation in the high-risk mucosotropic human papillomaviruses (HPVs). TtPV2 E6 contains a PDZ-binding motif that has been shown to be involved in transformation in the case of high-risk genital HPVs.
authors.affiliation: Harbor Branch Oceanographic Institution, Division of Marine Mammal Research and Conservation, 5600 US 1 North, Fort Pierce, FL 34946, USA.
authors.forename: Manuela
authors.initials: M
authors.lastname: Rehtanz
authors.affiliation: James Graham Brown Cancer Center, Laboratory of Vaccinology, University of Louisville, 529 South Jackson Street, Louisville, KY 40202, USA.|Harbor Branch Oceanographic Institution, Division of Marine Mammal Research and Conservation, 5600 US 1 North, Fort Pierce, FL 34946, USA.
authors.forename: Shin-Je
authors.initials: SJ
authors.lastname: Ghim
authors.affiliation: Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.
authors.forename: Annabel
authors.initials: A
authors.lastname: Rector
authors.affiliation: Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Van Ranst
authors.affiliation: National Oceanic and Atmospheric Administration/National Ocean Service/Center for Coastal Environmental Health and Biomolecular Research, Charleston, SC 29412, USA.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Fair
authors.affiliation: Harbor Branch Oceanographic Institution, Division of Marine Mammal Research and Conservation, 5600 US 1 North, Fort Pierce, FL 34946, USA.
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Bossart
authors.affiliation: James Graham Brown Cancer Center, Laboratory of Vaccinology, University of Louisville, 529 South Jackson Street, Louisville, KY 40202, USA.|Harbor Branch Oceanographic Institution, Division of Marine Mammal Research and Conservation, 5600 US 1 North, Fort Pierce, FL 34946, USA.
authors.forename: Alfred B
authors.initials: AB
authors.lastname: Jenson
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000818:Animals; D050681:Bottle-Nosed Dolphin / Q000821:virology*; D003001:Cloning, Molecular; D003218:Condylomata Acuminata / Q000662:veterinary* / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D016679:Genome, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification* / Q000302:isolation & purification*; D050725:Papillomavirus E7 Proteins / Q000235:genetics; D030361:Papillomavirus Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014481:United States; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D050725:Papillomavirus E7 Proteins; D014764:Viral Proteins