title: A real-time PCR approach to evaluate adipogenic potential of amniotic fluid-derived human mesenchymal stem cells.
abstract: Regulation of adipocyte differentiation is an important process in the control of adipose tissue development. So far, adipogenesis has been investigated through the use of various experimental models. In this work, we used human mesenchymal stem cells (hMSCs) obtained from amniotic fluid (AF) as an alternative model more representative of what naturally happens in vivo. In our opinion, these hMSCs are still not influenced by differentiation stimuli and could act in a way more correspondent to the physiological process of adipogenesis, representing also an ethically acceptable alternative to totipotent human embryonic stem cells (ES). Adipocyte differentiation was monitorated following the expressions of key genes. We measured the expression levels of PPARgamma2, PPARgamma-C1alpha, UCP-1, adipsin, and leptin genes using quantitative real-time PCR. We tested our experimental model with two different media. Understanding in vivo adipogenesis mechanisms will shed light on the pathophysiology of many diseases.
authors.affiliation: BIRD Europe Institute, Vicenza, Italy. paola.degemmis@birdfoundation.org
authors.forename: Paola
authors.initials: P
authors.lastname: De Gemmis
authors.forename: Cristina
authors.initials: C
authors.lastname: Lapucci
authors.forename: Matteo
authors.initials: M
authors.lastname: Bertelli
authors.forename: Anna
authors.initials: A
authors.lastname: Tognetto
authors.forename: Erika
authors.initials: E
authors.lastname: Fanin
authors.forename: Roberto
authors.initials: R
authors.lastname: Vettor
authors.forename: Claudio
authors.initials: C
authors.lastname: Pagano
authors.forename: Massimo
authors.initials: M
authors.lastname: Pandolfo
authors.forename: Andrea
authors.initials: A
authors.lastname: Fabbri
mesh_terms: D050156:Adipogenesis; D000653:Amniotic Fluid / Q000166:cytology*; D015415:Biomarkers; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
chemical_list: D015415:Biomarkers