title: Alterations of the retinoblastoma and p16 pathway correlate with promoter methylation in malignant fibrous histiocytomas.
abstract: Recent reports indicate that the alterations in the p16 and pRb pathways can influence tumour progression and poor prognosis in several tumours. The objective of this study was to analyse p16 and pRb expression in161 patients with malignant fibrous histiocytomas (MFH). By immunohistochemistry, p16 and pRb were demonstrated in 25% and 56% of MFH, respectively. Cox regression analysis demonstrated an independent prognostic influence of both genes. Generally, the loss of p16 and pRb expression correlated with poorer prognosis. Promoter methylation of p16 was found in 16/42 of p16 negative MFH and of pRb in 2/42 of pRb-negative MFH. It can be concluded that p16 and pRb alterations play an important role in the progression of soft tissue sarcomas.
authors.affiliation: Department of Pathology, Reinhard Nieter Hospital, Wilhelmshaven, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Brinck
authors.forename: Thilo
authors.initials: T
authors.lastname: Schlott
authors.forename: Steffi
authors.initials: S
authors.lastname: Störber
authors.forename: Jerzy
authors.initials: J
authors.lastname: Stachura
authors.forename: Pawel
authors.initials: P
authors.lastname: Bortkiewicz
authors.forename: Wolf-Dieter
authors.initials: WD
authors.lastname: Nagel
authors.forename: Frank Michael
authors.initials: FM
authors.lastname: Hasse
authors.forename: Carlos
authors.initials: C
authors.lastname: Cordon-Cardo
authors.forename: Gösta
authors.initials: G
authors.lastname: Fischer
authors.forename: Monika
authors.initials: M
authors.lastname: Korabiowska
mesh_terms: D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D051677:Histiocytoma, Malignant Fibrous / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D016160:Retinoblastoma Protein