title: Statistical analyses of correlation between fluconazole MICs for Candida spp. assessed by standard methods set forth by the European Committee on Antimicrobial Susceptibility Testing (E.Dis. 7.1) and CLSI (M27-A2).
abstract: The European Committee on Antimicrobial Susceptibility Testing (EUCAST) Subcommittee on Antifungal Susceptibility Testing recently published a standard for determining the susceptibility of fermentative yeasts to antifungals. From the beginning, the EUCAST and its North American counterpart, the CLSI, decided to work together in order to establish common standards. As part of this exercise, the susceptibility of a set of 475 yeast isolates was tested by both standards. The intraclass correlation coefficient and the equations defining the linear regression between both methods were estimated. Both methods produced very similar results, with an intraclass correlation coefficient of 0.954 (0.945 to 0.962), although linear regression analysis shows that the EUCAST standard resulted in slightly lower MICs. There were only eight isolates showing at least four twofold dilution MIC differences between both standards. After 24 h of incubation, the MICs obtained by the CLSI method were equivalent to those obtained by the EUCAST standard. In summary, both methods produce very similar MICs, indicating that methodology does not pose any obstacle to obtaining uniform standards for antifungal susceptibility testing of yeasts.
authors.affiliation: Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda Pozuelo km 2, 28220 Majadahonda, Madrid, Spain. juanl.rodriguez-tudela@isciii.es
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: Rodriguez-Tudela
authors.forename: J Peter
authors.initials: JP
authors.lastname: Donnelly
authors.forename: Michael A
authors.initials: MA
authors.lastname: Pfaller
authors.forename: Erja
authors.initials: E
authors.lastname: Chryssantou
authors.forename: Peter
authors.initials: P
authors.lastname: Warn
authors.forename: David W
authors.initials: DW
authors.lastname: Denning
authors.forename: Ana
authors.initials: A
authors.lastname: Espinel-Ingroff
authors.forename: Francesco
authors.initials: F
authors.lastname: Barchiesi
authors.forename: Manuel
authors.initials: M
authors.lastname: Cuenca-Estrella
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification / Q000187:drug effects*; D016001:Confidence Intervals; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards* / Q000706:statistics & numerical data; D012044:Regression Analysis
chemical_list: D000935:Antifungal Agents; D015725:Fluconazole