Low-level quantitation of oxycodone and its oxidative metabolites, noroxycodone, and oxymorphone, in rat plasma by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry.
A method was developed for quantification of oxycodone, noroxycodone, and oxymorphone in small volumes (50 microl) of rat plasma by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry using turbo ion-spray. Deuterated (d3) opioid analogues acted as internal standards. Sample preparation involved protein precipitation with acetonitrile, centrifugal evaporation, and reconstitution in mobile phase; analyte separation was performed on a C18 (5 microm, 2.1 mm x 50 mm) column using a linear gradient program. Lower limits of quantitation (ng/ml) and their between-day accuracy and precision were-oxycodone, 0.9 (-0.2 and 7.8%); noroxycodone, 1.0 (0.6 and 6.2%); oxymorphone 1.0 (-1.8 and 9.5%).
Edwards
Stephen R
SR
School of Pharmacy, The University of Queensland, St. Lucia, Brisbane, Qld. 4072, Australia.
Smith
Maree T
MT
D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007275:Injections, Intravenous; D008297:Male; D015394:Molecular Structure; D009019:Morphinans / Q000097:blood* / Q000737:chemistry; D010098:Oxycodone / Q000097:blood* / Q000737:chemistry; D010111:Oxymorphone / Q000008:administration & dosage / Q000097:blood* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
D000701:Analgesics, Opioid; D009019:Morphinans; C077256:noroxycodone; D010111:Oxymorphone; D010098:Oxycodone