Skin penetration flux and lag-time of steroids across hydrated and dehydrated human skin in vitro.
To study the effect of hydration on skin absorption, we investigated penetration across human skin of twelve model chemicals having steroidal structure but different molecular weight and compared the steady-state penetration rate (J) and lag-time (t) across hydration intact skin (Jh and th) with that across dehydrated intact skin (Jd and td). Stratum corneum (SC) thickness of hydrated (52 microm) is 3.3 times that of dehydrated skin (16 microm). Transepidermal water loss (TEWL) of hydrated (7.6+/-2.1 g/m2/h) is twice that of dehydrated skin (3.4+/-1.6 g/m2/h, p<0.05) which are similar to in vivo values, suggesting the SC barrier function was recovered. The ratio of Jh/Jd ranged between 0.7 and 3.6 (average of 1.9). On the other hand, the ratio of th/td was almost constant (average of 0.8). Ratios of Jh/Jd and th/td were independent of MW and Ko/w. In percutaneous absorption experiments in vitro, skin was preserved in culture medium until use and SC might swell during that time. Therefore, we consider the possibility that J and t varied between hydrated and dehydrated skin. We confirmed the difference of J and t between hydrated and dehydrated skin in vitro and now need to define these results under in vivo condition.
Hikima
Tomohiro
T
Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Fukuoka, Japan. hikima@bio.kyutech.ac.jp
Maibach
Howard
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D003681:Dehydration / Q000503:physiopathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D012867:Skin / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D012869:Skin Absorption; D013256:Steroids / Q000737:chemistry / Q000493:pharmacokinetics*; D013997:Time Factors; D046509:Tissue Culture Techniques; D014867:Water / Q000494:pharmacology; D014870:Water Loss, Insensible / Q000187:drug effects / Q000502:physiology
D013256:Steroids; D014867:Water