title: Quantitative analysis of plasma DNA in severe acute pancreatitis.
abstract: BACKGROUND
  Release of genomic DNA into plasma as a result of necrotic and apoptotic pathways is a feature of a range of human tumours. Severe acute pancreatitis is characterized by inflammation but may also be associated with accelerated apoptotic and necrotic pathways.
  
  
  OBJECTIVE
  This study uses quantitative real-time PCR to measure free circulating DNA in patients with severe acute pancreatitis.
  
  
  METHODS
  Forty-three patients with severe acute pancreatitis, 12 patients with pancreatic cancer and 28 non-cancer controls undergoing laparoscopic cholecystectomy.
  
  
  METHODS
  Plasma DNA was purified and quantified using the RNase P transcription assay and quantitative PCR. In pancreatitis patients, baseline samples were taken on admission and further samples taken at a median of 5 days into the disease course.
  
  
  RESULTS
  Plasma DNA levels on admission in patients with acute pancreatitis (median: 0.40 ng/microL; range: 0.05-0.79 ng/microL) were significantly (P<0.001) lower than in non-cancer controls (median: 1.60 ng/microL; range: 0.45-9.10 ng/microL). In patients with acute pancreatitis, DNA levels significantly (P<0.001) fell during the disease course to a median value of 0.08 ng/microL (range: 0-0.53 ng/microL).
  
  
  CONCLUSIONS
  This is the first study to use quantitative PCR to measure free plasma DNA in severe acute pancreatitis. The results show that plasma DNA is lower in patients with acute pancreatitis compared to control and that values fall further during the disease course.
authors.affiliation: HPB Unit, Department of Surgery, Manchester Royal Infirmary, Manchester, United Kingdom.
authors.forename: Anil
authors.initials: A
authors.lastname: Bagul
authors.forename: Sudeep
authors.initials: S
authors.lastname: Pushpakom
authors.forename: James
authors.initials: J
authors.lastname: Boylan
authors.forename: William
authors.initials: W
authors.lastname: Newman
authors.forename: Ajith K
authors.initials: AK
authors.lastname: Siriwardena
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004247:DNA / Q000097:blood*; D005260:Female; D006760:Hospitalization; D006801:Humans; D008297:Male; D008875:Middle Aged; D009336:Necrosis / Q000097:blood; D017063:Outcome Assessment, Health Care; D010195:Pancreatitis / Q000097:blood* / Q000473:pathology
chemical_list: D004247:DNA