Detailed consideration of physicochemical properties of CO3apatites as biomaterials in relation to carbonate content using ICP, X-ray diffraction, FT-IR, SEM, and HR-TEM.
CO3apatites with different carbonate contents were synthesized at 60 +/- 1 degrees C and pH 7.4 +/- 0.2 under different carbonate concentrations (0-0.3 mol/L) in the supplied solutions. Their physicochemical properties were analyzed using various methods. Inductively coupled plasma gave accurate chemical analysis data for calcium and phosphate contents. X-ray diffraction analysis showed a clear chemical shift at high carbonate content. A CO3(2-) absorption peak area approximately proportional to carbonate content was observed through Fourier transmission infrared spectroscopy. Scanning electron microscopy and high-resolution transmission electron microscopy revealed a dramatic change of the crystal shape. Osteoblast proliferation at the surface of each CO3apatite-collagen sponge indicated that osteoblasts deformed to expand and cover the surface of the sponge, and appeared to adhere well to the sponge.
Yokota
Rie
R
Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University, Japan.
Hayashi
Hidetaka
H
Hirata
Isao
I
Miake
Yasuo
Y
Yanagisawa
Takaaki
T
Okazaki
Masayuki
M
D000818:Animals; D001031:Apatites / Q000138:chemical synthesis / Q000737:chemistry*; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002254:Carbonates / Q000737:chemistry*; D002417:Cattle; D051379:Mice; D046529:Microscopy, Electron, Transmission; D010006:Osteoblasts / Q000737:chemistry; D010949:Plasma; D012996:Solutions / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
D001031:Apatites; D001672:Biocompatible Materials; D002254:Carbonates; D012996:Solutions; C030782:carboapatite