title: [Effect of calpain inhibitor I on ikappaBalpha expression and cytokine secretion in RAW264.7 cells attacked with LPS].
abstract: OBJECTIVE
  To explore the effect of calpain inhibitor I(CI-I) on ikappaBalpha expression and cytokine secretion in RAW264.7 cells after LPS attack.
  
  
  METHODS
  RAW264.7 cells were pretreated with CI-I for 1 h and attacked with LPS. The ikappaBalpha expression and TNF-alpha and IL-6 secretion were detected by Western blot and ELISA, respectively.
  
  
  RESULTS
  CI-I inhibited the decrease of ikappaBalpha expression in RAW264.7 cells treated with LPS. The secretion of both TNF-alpha and IL-6 secretion was notably increased after RAW264.7 cells were treated with LPS for 4 h or 8 h. CI-I and dexamethasone(DEX) inhibited these effects, and the combination of DEX and CI-I had synergistic effect.
  
  
  CONCLUSIONS
  CI-I and DEX can inhibit the decrease of ikappaBalpha expression and prevent TNF-alpha and IL-6 secretion in RAW264.7 cells attacked with LPS, which contributes to the alleviation of cellular injury.
authors.affiliation: Institute of Combined Injury, Laboratory of Combined Injury of State Key Labortory of Trauma, Burn and Combined Injury, Department of Preventive Medcine, Third Military Medical University, Chongqing 400038, China. xiaogangchen@yahoo.com
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Cheng
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Su
authors.forename: Cheng-Ji
authors.initials: CJ
authors.lastname: Luo
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Liu
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006023:Glycoproteins / Q000494:pharmacology*; D020672:I-kappa B Proteins / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D016207:Cytokines; D006023:Glycoproteins; D020672:I-kappa B Proteins; D008070:Lipopolysaccharides; C000606205:Nfkbia protein, mouse; D012333:RNA, Messenger; C039304:calpain inhibitors; D000072000:NF-KappaB Inhibitor alpha; D003907:Dexamethasone; D002118:Calcium