[Study on the genetic polymorphisms of human glutathione-S-transferase A1 in Hakka population in South China].
OBJECTIVE
Glutathione-S-transferase A1 (GSTA1) is one of the major phase II detoxification enzymes in the cytosol, which genetic polymorphisms distribution is different in different ethnic, national and regional population. Up to now, GSTA1 genetic polymorphisms has been rarely reported in China. The purpose of the study was to investigate the distribution of genetic polymorphisms of human GSTA1 in Hakka population in South China.


METHODS
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were used to identify the genotypes of GSTA1 gene and the data were analyzed with SPSS10.0 software.


RESULTS
The GSTA1 genetic polymorphisms were detected in 480 samples. The frequency of GSTA1 * A/ * A,GSTA1 * A/ * B and GSTA1 * B/ * B were 77.1%, 21.7% and 1.2% respectively. And the GSTA1 genetic polymorphism distribution was in accordance with the Hardy-Weinberg equilibrium rule. There were no difference in the GSTA1 genetic polymorphisms among the different groups of age or gender. Logistic regression analysis showed that there were no association between the GSTA1 genetic polymorphism and family history of hypertension, coronary heart disease, stroke, lung cancer and nasopharyngeal cancer, et al.


CONCLUSIONS
The GSTA1 gene existed polymorphism among Hakka in South China.
Pan
Shang-Xia
SX
Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, China.
Yang
Xing-Fen
XF
Lin
Zhong-Ning
ZN
Yang
Yi-Long
YL
Zhao
Min
M
Xu
Guang-ning
GN
Huang
Jun-ming
JM
Wei
Qing
Q
Ling
Wen-hua
WH
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
C484555:GSTA1 protein, human; D005982:Glutathione Transferase