Effects of addition of sodium lauryl sulfate on frozen-thawed canine spermatozoa.
The addition of Orvus ES paste (OEP) to extender may be essential for preparing frozen dog semen. The major ingredient of OEP is sodium lauryl sulfate (SLS). In this study, we compared the effect of SLS on frozen dog semen with that of OEP. There were no significant differences between the 2-mg/ml SLS group and OEP group concerning sperm motility, viability and the percentage of viable sperm with intact acrosomes after freeze-thawing. These results suggest that the effectiveness of frozen dog semen extender containing 2 mg/ml of SLS is similar effective to that demonstrated for OEP.
Hori
Tatsuya
T
Department of Reproduction, Nippon Veterinary and Life Science University, Tokyo, Japan.
Kaseki
Hanae
H
Fukuhara
Youko
Y
Oba
Hiromichi
H
Mizutani
Tatsuji
T
Kawakami
Eiichi
E
Tsutsui
Toshihiko
T
D000177:Acrosome / Q000502:physiology; D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000502:physiology*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012967:Sodium Dodecyl Sulfate; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*
D012967:Sodium Dodecyl Sulfate