Rhizobium etli USDA9032 engineered to produce a phenazine antibiotic inhibits the growth of fungal pathogens but is impaired in symbiotic performance.
Phenazine production was engineered in Rhizobium etli USDA9032 by the introduction of the phz locus of Pseudomonas chlororaphis O6. Phenazine-producing R. etli was able to inhibit the growth of Botrytis cinerea and Fusarium oxysporum in vitro. Black bean inoculated with phenazine-producing R. etli produced brownish Fix(-) nodules.
Krishnan
Hari B
HB
Plant Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service, 108W Curtis Hall, University of Missouri, Columbia, MO 65211, USA. KrishnanH@missouri.edu
Kang
Beom Ryong
BR
Hari Krishnan
Ammulu
A
Kim
Kil Yong
KY
Kim
Young Cheol
YC
D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D020171:Botrytis / Q000235:genetics / Q000254:growth & development; D007887:Fabaceae / Q000382:microbiology; D005658:Fungi / Q000254:growth & development* / Q000472:pathogenicity; D005670:Fusarium / Q000235:genetics / Q000254:growth & development; D005818:Genetic Engineering / Q000379:methods*; D010572:Pest Control, Biological / Q000379:methods; D010619:Phenazines / Q000378:metabolism* / Q000494:pharmacology; D042163:Rhizobium etli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D013559:Symbiosis / Q000187:drug effects
D000900:Anti-Bacterial Agents; D010619:Phenazines