First observation of cylindrospermopsin in Anabaena lapponica isolated from the boreal environment (Finland).
The cyanobacterial cytotoxin cylindrospermopsin has been mostly associated with cyanobacteria present in tropical and subtropical regions. Cylindrospermopsin has recently been found in cyanobacterial samples in central and southern Europe but the possible presence of the toxin in northern Europe has been unknown. Fifty-eight field and laboratory culture samples of Finnish cyanobacteria were analyzed by high-performance liquid chromatography combined with UV diode-array detection, multiple reactant monitoring in a triple-quadrupole mass spectrometer (MS), and accurate mass measurements using a time-of-flight MS instrument. Cylindrospermopsin was confirmed by all three techniques in a culture sample of Anabaena lapponica at a concentration of 242 microg cylindrospermopsin per g freeze-dried cyanobacterial material.
Spoof
Lisa
L
Department of Biochemistry and Pharmacy, Abo Akademi University, Tykistökatu 6A, FI-20520 Turku, Finland.
Berg
Katri A
KA
Rapala
Jarkko
J
Lahti
Kirsti
K
Lepistö
Liisa
L
Metcalf
James S
JS
Codd
Geoffrey A
GA
Meriluoto
Jussi
J
D000470:Alkaloids; D017033:Anabaena / Q000737:chemistry / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D001427:Bacterial Toxins; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D000087522:Cyanobacteria Toxins; D005387:Finland; D005612:Freeze Drying / Q000379:methods; D005618:Fresh Water / Q000382:microbiology*; D013058:Mass Spectrometry / Q000379:methods; D012623:Seawater / Q000382:microbiology*; D014498:Uracil / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism
D000470:Alkaloids; D001427:Bacterial Toxins; D003470:Culture Media; D000087522:Cyanobacteria Toxins; C089595:cylindrospermopsin; D014498:Uracil