Phospholipid fatty acid pattern and D-glucose metabolism in muscles from omega3 fatty acid-depleted rats.
A depletion in long-chain polyunsaturated omega3 fatty acids may affect fuel homeostasis. In such a perspective, the present study deals mainly with the in vitro fate of D-[U-(14)C]glucose in hemidiaphragms, stretched soleus and plantaris muscle pieces obtained from normal and omega3-depleted rats (second generation) and incubated in the absence or presence of insulin. When so required, the omega3-depleted rats were injected 120 min before sacrifice with either a omega3 fatty acid-rich medium-chain triglyceride:fish oil emulsion (FO) or a control medium-chain triglyceride:olive oil emulsion (OO). The content of the soleus muscle in long-chain polyunsaturated omega3 fatty acids was severely decreased in the omega3-depleted rats, and modestly albeit significantly increased after injection of FO to these animals. In stretched soleus muscles from OO-injected omega3-depleted rats, the absolute values for glycogen synthesis measured in the absence or presence of insulin were about twice higher than in normal animals. In the OO-injected omega3-depleted rats, insulin augmented the output of (14)C-labelled amino acids, whilst such was not the case in normal animals. These and other findings suggest a lower catabolism of D-glucose relative to the anabolic process of glycogen synthesis and a lower availability of endogenous amino acids in the muscles of omega3-depleted rats, as compared to those of control animals. The prior injection of FO to the omega3-depleted rats restored a normal value for the paired ratio between the output of (14)C-labelled amino acids and acidic metabolites, but further increased glycogen net synthesis. It is proposed, therefore, that the perturbation of d-glucose metabolism in muscles from omega3-depleted rats involves a multifactorial determinism, only some of the concerned factors being susceptible to rapid correction after enrichment of cell phospholipids in long-chain polyunsaturated omega3 fatty acids.
Agascioglu
Eda
E
Laboratory of Experimental Hormonology, Brussels Free University, 808 Route de Lennik, 1070 Brussels, Belgium.
Zhang
Ying
Y
Sener
Abdullah
A
Portois
Laurence
L
Chardigny
Jean-Michel
JM
Malaisse
Willy J
WJ
Carpentier
Yvon A
YA
D000818:Animals; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects; D009132:Muscles / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D000069463:Olive Oil; D010743:Phospholipids / Q000737:chemistry*; D010938:Plant Oils / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D014280:Triglycerides / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism
D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D007328:Insulin; D000069463:Olive Oil; D010743:Phospholipids; D010938:Plant Oils; D014280:Triglycerides; D006003:Glycogen; D005947:Glucose