title: The genome of deep-sea vent chemolithoautotroph Thiomicrospira crunogena XCL-2.
abstract: Presented here is the complete genome sequence of Thiomicrospira crunogena XCL-2, representative of ubiquitous chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 base pairs), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. It has 14 methyl-accepting chemotaxis protein genes, including four that may assist in positioning it in the redoxcline. A relative abundance of coding sequences (CDSs) encoding regulatory proteins likely control the expression of genes encoding carboxysomes, multiple dissolved inorganic nitrogen and phosphate transporters, as well as a phosphonate operon, which provide this species with a variety of options for acquiring these substrates from the environment. Thiom. crunogena XCL-2 is unusual among obligate sulfur-oxidizing bacteria in relying on the Sox system for the oxidation of reduced sulfur compounds. The genome has characteristics consistent with an obligately chemolithoautotrophic lifestyle, including few transporters predicted to have organic allocrits, and Calvin-Benson-Bassham cycle CDSs scattered throughout the genome.
authors.affiliation: Biology Department, University of South Florida, Tampa, Florida, United States of America. kscott@cas.usf.edu
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Scott
authors.forename: Stefan M
authors.initials: SM
authors.lastname: Sievert
authors.forename: Fereniki N
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authors.lastname: Abril
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authors.lastname: Ball
authors.forename: Chantell J
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authors.lastname: Barrett
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Blake
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Boller
authors.forename: Patrick S G
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authors.lastname: Chain
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authors.forename: Carisa R
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authors.lastname: Davis
authors.forename: Chris
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authors.lastname: Detter
authors.forename: Kimberly F
authors.initials: KF
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authors.forename: Kimberly P
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authors.lastname: Dobrinski
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authors.lastname: Faza
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authors.initials: KA
authors.lastname: Fitzpatrick
authors.forename: Sharyn K
authors.initials: SK
authors.lastname: Freyermuth
authors.forename: Tara L
authors.initials: TL
authors.lastname: Harmer
authors.forename: Loren J
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authors.lastname: Hauser
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authors.lastname: Hügler
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Kerfeld
authors.forename: Martin G
authors.initials: MG
authors.lastname: Klotz
authors.forename: William W
authors.initials: WW
authors.lastname: Kong
authors.forename: Miriam
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authors.lastname: Land
authors.forename: Alla
authors.initials: A
authors.lastname: Lapidus
authors.forename: Frank W
authors.initials: FW
authors.lastname: Larimer
authors.forename: Dana L
authors.initials: DL
authors.lastname: Longo
authors.forename: Susan
authors.initials: S
authors.lastname: Lucas
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Malfatti
authors.forename: Steven E
authors.initials: SE
authors.lastname: Massey
authors.forename: Darlene D
authors.initials: DD
authors.lastname: Martin
authors.forename: Zoe
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authors.lastname: McCuddin
authors.forename: Folker
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authors.lastname: Meyer
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Moore
authors.forename: Luis H
authors.initials: LH
authors.lastname: Ocampo
authors.forename: John H
authors.initials: JH
authors.lastname: Paul
authors.forename: Ian T
authors.initials: IT
authors.lastname: Paulsen
authors.forename: Douglas K
authors.initials: DK
authors.lastname: Reep
authors.forename: Qinghu
authors.initials: Q
authors.lastname: Ren
authors.forename: Rachel L
authors.initials: RL
authors.lastname: Ross
authors.forename: Priscila Y
authors.initials: PY
authors.lastname: Sato
authors.forename: Phaedra
authors.initials: P
authors.lastname: Thomas
authors.forename: Lance E
authors.initials: LE
authors.lastname: Tinkham
authors.forename: Gary T
authors.initials: GT
authors.lastname: Zeruth
mesh_terms: D001422:Bacterial Adhesion / Q000235:genetics; D002245:Carbon Dioxide / Q000378:metabolism; D002633:Chemotaxis / Q000235:genetics; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism; D044147:Piscirickettsiaceae / Q000235:genetics* / Q000378:metabolism; D039002:Prophages / Q000235:genetics; D016415:Sequence Alignment; D015398:Signal Transduction
chemical_list: D010710:Phosphates; D002245:Carbon Dioxide