title: cDNA microarray analysis of gene expression in Brassica napus treated with oligochitosan elicitor.
abstract: An oilseed rape (Brassica napus H) cDNA microarray containing 8095 expressed sequence tags (ESTs) was used to analyze the B. napus gene expression changes elicited by oligochitosan. Transcript levels for 393 genes were altered twofold or more in oligochitosan-treated seedlings compared to control seedlings. Of the 393 genes, 257 were repressed and 136 were induced. Semi quantification RT-PCR of eight of these 393 genes confirmed the microarray results. These 393 genes were involved in different processes and had different functions including defense, primary metabolism, transcription, and signal transduction etc. Some of these genes were elicited by various pathogen-related or stress stimuli, and others were regulated by plant growth regulators like auxin and gibberellin. These manifested complicated interactions of oligochitosan and phytohormones. An important jasmonic acid (JA) synthase (2-oxophytodienoate-10,11-reductase) gene, a JA-mediated defense required kinase ATMPK4-homolog gene, an ethylene receptor gene, and two ethylene responsive element binding protein (EREBP) genes were induced by oligochitosan, suggesting that oligochitosan activated the plant self-defense through JA/ET signaling pathway.
authors.affiliation: Biotechnology Department, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, China. dyguang@gmail.com
authors.forename: H
authors.initials: H
authors.lastname: Yin
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: Du
authors.forename: X
authors.initials: X
authors.lastname: Ma
mesh_terms: D029688:Brassica napus / Q000235:genetics / Q000378:metabolism*; D048271:Chitosan / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D009844:Oligosaccharides / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D009844:Oligosaccharides; D010937:Plant Growth Regulators; D010940:Plant Proteins; D048271:Chitosan