title: Electrochemical detection of wild type Saccharomyces cerevisiae responses to estrogens.
abstract: The presence of an estrogen binding protein (EBP) and an endogenous ligand in three yeast species was first reported in 1982/1983. The ligand was shown to be 17beta-estradiol and the binding affinities of EBP were demonstrated to be similar to those of rat estrogen receptors. This report describes detection of the behaviour of a putative estrogen binding protein in Saccharomyces cerevisiae using a double mediator electrochemical detection system. The response to estrogen is shown to be quantitative with signals detectable from 10(-8) to 10(-14)M. An incubation period of 5h is established and a method to block electrochemical signals produced by the catabolism of exogenous substrates is demonstrated to be effective. The system provides a method that permits the use of wild type S. cerevisiae to quantify estrogens.
authors.affiliation: School of Applied Science, Christchurch Polytechnic Institute of Technology, P.O. Box 540, Christchurch, New Zealand. baroniank@cpit.ac.nz <baroniank@cpit.ac.nz>
authors.forename: Keith H R
authors.initials: KH
authors.lastname: Baronian
authors.forename: Saroja
authors.initials: S
authors.lastname: Gurazada
mesh_terms: D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000379:methods*; D004967:Estrogens / Q000008:administration & dosage* / Q000032:analysis*; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000502:physiology*; D012680:Sensitivity and Specificity
chemical_list: D004967:Estrogens