title: Aph(3')-IIc, an aminoglycoside resistance determinant from Stenotrophomonas maltophilia.
abstract: We report the characterization of an intrinsic, chromosomally carried aph(3')-IIc gene from Stenotrophomonas maltophilia clinical isolate K279a, encoding an aminoglycoside phosphotransferase enzyme that significantly increases MICs of kanamycin, neomycin, butirosin, and paromomycin when expressed in Escherichia coli. Disruption of aph(3')-IIc in K279a results in decreased MICs of these drugs.
authors.affiliation: Department of Cellular and Molecular Medicine, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom.
authors.forename: Aki
authors.initials: A
authors.lastname: Okazaki
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Avison
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002076:Butirosin Sulfate / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D007612:Kanamycin / Q000494:pharmacology; D019868:Kanamycin Kinase / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009355:Neomycin / Q000494:pharmacology; D020615:Stenotrophomonas maltophilia / Q000187:drug effects* / Q000235:genetics
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002076:Butirosin Sulfate; D007612:Kanamycin; D019868:Kanamycin Kinase; D009355:Neomycin