Additional antiprotozoal flavonol glycosides of the aerial parts of Helianthemum glomeratum.
Bioassay-guided fractionation of the methanol extract of aerial parts from Helianthemum glomeratum afforded five antiprotozoal flavonol glycosides: tiliroside, kaempferol-3-O-(3'',6''di-O-E-p-coumaroyl)-betad-glucopyranoside, astragalin, quercitrin and isoquercitrin. The in vitro antiprotozoal assay showed that tiliroside was the most potent antiamoebic and antigiardial compound with IC(50) values of 17.5 microg/mL for Entamoeba histolytica and 17.4 microg/mL for G. lamblia. Isoquercitrin showed selectivity against E. histolytica (IC(50) 14.7 microg/mL) and quercitrin toward G. lamblia (IC(50) 24.3 microg/mL). All isolated compounds were less active than metronidazole and emetine, two antiprotozoal drugs used as positive controls.
Calzada
Fernando
F
Unidad de Investigación Médica en Farmacología de Productos Naturales, Centro Médico Nacional Siglo XXI, IMSS, 06725, México D. F., México. fercalber1@hotmail.com
Alanís
Alma Delia
AD
D000818:Animals; D000981:Antiprotozoal Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D030601:Cistaceae; D004748:Entamoeba histolytica / Q000187:drug effects; D044948:Flavonols / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D016829:Giardia lamblia / Q000187:drug effects; D006027:Glycosides / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D021261:Parasitic Sensitivity Tests; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D000981:Antiprotozoal Agents; D044948:Flavonols; D006027:Glycosides; D010936:Plant Extracts