title: Anti-KEL7 (anti-Js(b)) alloimmunization diagnostic supported by molecular KEL*6,7 typing in a pregnant woman with previous intrauterine deaths.
abstract: Anti-KEL7 (anti-Js(b)) is a rare antibody that has been related to haemolytic transfusion reactions and HDN. We report a case of anti-KEL7 alloimmunization detected in a pregnant woman who had an obstetric previous history of four miscarriages and one stillborn. Employing classical immunohematological techniques, we studied the propositus and her available relatives. Due to the unavailability of commercial anti-KEL6 and anti-KEL7 reagents, we used a KEL*6,7 genotyping method as an alternative tool to contribute with the identification of the alloantibody origin. The results of KEL genotyping showed that the propositus was KEL*6/6 homozygous, while her second partner was KEL*7/7 homozygous.
authors.affiliation: Disciplina de Hematologia e Hemoterapia, Universidade Federal de São Paulo, Escola Paulista de Medicina, Rua Botucatu, 740, São Paulo 04023-092, SP, Brazil.
authors.forename: Edmir
authors.initials: E
authors.lastname: Boturão-Neto
authors.forename: Akemi Kuroda
authors.initials: AK
authors.lastname: Chiba
authors.forename: Melca Maria
authors.initials: MM
authors.lastname: Oliveira Barros
authors.forename: Adriana
authors.initials: A
authors.lastname: Barretto de Mello
authors.forename: Antonio
authors.initials: A
authors.lastname: Fabron
authors.forename: José
authors.initials: J
authors.lastname: Orlando Bordin
mesh_terms: D000328:Adult; D001787:Blood Group Incompatibility / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D002675:Child, Preschool; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005313:Fetal Death / Q000235:genetics*; D005838:Genotype; D006720:Homozygote; D006801:Humans; D007231:Infant, Newborn; D007518:Isoantibodies / Q000097:blood* / Q000235:genetics; D007626:Kell Blood-Group System / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011248:Pregnancy Complications / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology*; D018566:Pregnancy, High-Risk / Q000276:immunology; D065227:Transfusion Reaction
chemical_list: D007518:Isoantibodies; D007626:Kell Blood-Group System