Pseudomonas simiae sp. nov., isolated from clinical specimens from monkeys (Callithrix geoffroyi).
An unusual Gram-negative, catalase- and oxidase-positive, rod-shaped bacterium isolated from different clinical samples from two monkeys (Callithrix geoffroyi) was characterized by phenotypic and molecular genetic methods. The micro-organism was tentatively identified as a Pseudomonas species on the basis of the results of cellular morphological and biochemical tests. Fatty acid studies confirmed this generic placement and comparative 16S rRNA gene sequencing studies demonstrated that the unknown isolates were phylogenetically closely related to each other (100% sequence similarity) and were part of the 'Pseudomonas fluorescens intrageneric cluster'. The novel bacterium, however, was distinguished from other phylogenetically related species of Pseudomonas by DNA-DNA hybridization studies and biochemical tests. On the basis of both phenotypic and phylogenetic findings, it is proposed that the novel Pseudomonas isolates are classified as Pseudomonas simiae sp. nov. The type strain of P. simiae is OLiT (=CCUG 50988T=CECT 7078T).
Vela
Ana I
AI
Departamento Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Avda Puerta de Hierro s/n, 28040 Madrid, Spain.
Gutiérrez
María C
MC
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Sevilla, Spain.
Falsen
Enevold
E
Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Sweden.
Rollán
Eduardo
E
Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Simarro
Isabel
I
Departamento Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Avda Puerta de Hierro s/n, 28040 Madrid, Spain.
García
Pilar
P
Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Domínguez
Lucas
L
Departamento Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Avda Puerta de Hierro s/n, 28040 Madrid, Spain.
Ventosa
Antonio
A
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Sevilla, Spain.
Fernández-Garayzábal
José F
JF
Departamento Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Avda Puerta de Hierro s/n, 28040 Madrid, Spain.
D000818:Animals; D015373:Bacterial Typing Techniques; D002144:Callithrix / Q000382:microbiology*; D050260:Carbohydrate Metabolism; D002244:Carbon / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D018093:Gelatinases / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000382:microbiology*; D009566:Nitrates / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification*; D011552:Pseudomonas Infections / Q000382:microbiology / Q000662:veterinary*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D010088:Oxidoreductases; D002374:Catalase; D018093:Gelatinases