Expressed sequence tags from feline uterine library.
Partial nucleotide sequences of 634 cDNAs randomly isolated from a feline uterine cDNA library (Stratagene) were determined by single pass sequencing. Homology search of the sequences to the non-redundant nucleotide databases revealed that 83% of the cDNAs matched registered feline or non-feline genes. Based on the gene identifications, these genes were predicted to be related with immunological, biochemical and regulatory functions in cats. Interestingly, the rest 17% of the cDNAs did not show homology to gene or EST sequence present in the nucleotide and protein databases, suggesting that these cDNAs include novel genes expressed only in the Felidae. This large scale sequencing of uterine cDNA will provide a useful molecular source for research not only towards health and disease conditions in cats but also in different fields of science where genetic information from cats will be of interest.
Pathak
Simanta
S
Louise C. Averill Feline Research Laboratory, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, 1800 Denison Ave, Manhattan, KS 66506, USA.
Kapil
Sanjay
S
D000818:Animals; D001483:Base Sequence; D002415:Cats / Q000235:genetics*; D030561:Databases, Nucleic Acid; D004717:Endometrium / Q000378:metabolism*; D020224:Expressed Sequence Tags; D005260:Female; D015723:Gene Library; D023281:Genomics