title: Transcriptional interference among the murine beta-like globin genes.
abstract: Mammalian beta-globin loci contain multiple genes that are activated at different developmental stages. Studies have suggested that the transcription of one gene in a locus can influence the expression of the other locus genes. The prevalent model to explain this transcriptional interference is that all potentially active genes compete for locus control region (LCR) activity. To investigate the influence of transcription by the murine embryonic genes on transcription of the other beta-like genes, we generated mice with deletions of the promoter regions of Ey and betah1 and measured transcription of the remaining genes. Deletion of the Ey and betah1 promoters increased transcription of betamajor and betaminor 2-fold to 3-fold during primitive erythropoiesis. Deletion of Ey did not affect betah1 nor did deletion of betah1 affect Ey, but Ey deletion uniquely activated transcription from betah0, a beta-like globin gene immediately downstream of Ey. Protein analysis showed that betah0 encodes a translatable beta-like globin protein that can pair with alpha globin. The lack of transcriptional interference between Ey and betah1 and the gene-specific repression of betah0 did not support LCR competition among the embryonic genes and suggested that direct transcriptional interference from Ey suppressed betah0.
authors.affiliation: Department of Microbiology/Immunology and Norris Cotton Cancer Center, Dartmouth Medical School, Hanover, NH 03756, USA.
authors.forename: Xiao
authors.initials: X
authors.lastname: Hu
authors.forename: Susan
authors.initials: S
authors.lastname: Eszterhas
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pallazzi
authors.forename: Eric E
authors.initials: EE
authors.lastname: Bouhassira
authors.forename: Jennifer
authors.initials: J
authors.lastname: Fields
authors.forename: Osamu
authors.initials: O
authors.lastname: Tanabe
authors.forename: Scott A
authors.initials: SA
authors.lastname: Gerber
authors.forename: Michael
authors.initials: M
authors.lastname: Bulger
authors.forename: James Douglas
authors.initials: JD
authors.lastname: Engel
authors.forename: Mark
authors.initials: M
authors.lastname: Groudine
authors.forename: Steven
authors.initials: S
authors.lastname: Fiering
mesh_terms: D000818:Animals; D004920:Erythropoiesis; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D005914:Globins / Q000235:genetics*; D051379:Mice; D011401:Promoter Regions, Genetic / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D005136:Eye Proteins; D005914:Globins