Expression and effect of adrenomedullin in pheochromocytoma.
This study investigates the expression of human adrenomedullin (ADM) and its receptor-receptor activity modifying protein 2/calcitonin receptor-like receptor (RAMP2/CRLR) mRNA in pheochromocytoma by reverse transcriptase polymerase chain reaction (RT-PCR) and its effect on the proliferation of pheochromocytoma cells by MTT. The mRNA expression of ADM and its receptor RAMP2/CRLR was present in normal adrenal medulla and pheochromocytoma tissues. The mRNA expression of ADM, RAMP2, and CRLR is markedly higher in pheochromocytomas than in normal medulla. ADM inhibits the proliferation of human pheochromocytoma cells and exerts a possible autocrine or paracrine effect in the adrenal.
Zeng
Zheng-Pei
ZP
Department of Endocrinology, Peking Union Medical College Hospital, Beijing 100730, China. zengzhengpei@yahoo.com.cn
Liu
Dong-Mei
DM
Li
Han-Zhong
HZ
Fan
Xin-Rong
XR
Liu
Guo-Qiang
GQ
Yan
Wei-Gang
WG
Tong
An-Li
AL
Zheng
Xin
X
D000310:Adrenal Gland Neoplasms / Q000235:genetics*; D053607:Adrenomedullin / Q000235:genetics*; D000328:Adult; D001483:Base Sequence; D058286:Calcitonin Receptor-Like Protein; D017931:DNA Primers; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008875:Middle Aged; D010673:Pheochromocytoma / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D058263:Receptor Activity-Modifying Protein 2; D058261:Receptor Activity-Modifying Proteins; D018003:Receptors, Calcitonin / Q000235:genetics
C545578:CALCRL protein, human; D058286:Calcitonin Receptor-Like Protein; D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C545503:RAMP2 protein, human; D012333:RNA, Messenger; D058263:Receptor Activity-Modifying Protein 2; D058261:Receptor Activity-Modifying Proteins; D018003:Receptors, Calcitonin; D053607:Adrenomedullin