Biologic control ability of plant growth-promoting Paenibacillus lentimorbus NRRL B-30488 isolated from milk.
A plant growth-promoting Paenibacillus lentimorbus NRRL B-30488 (B-30488) was isolated from cows' milk. Bacterial colonization and growth responses of different plant species after inoculation with B-30488 were evaluated in a controlled environment and in microplot assays. Survival and colonization of B-30488 in the phytosphere of plants and soil was monitored using a chromosomally located rifampicin-marked mutant B-30488 (B-30488R). The strain showed variable ability to invade plants. The interaction between B-30488R and Fusarium oxysporum f. sp. ciceri was studied by scanning electron microscopy. Chitinase and beta-1,3-glucanase enzymes were produced when B-30488R was grown in the presence of colloidal chitin as sole carbon source. Deliberate dilution of B-30488R with field soil offers a reliable process for decreasing the cost of bacterial inoculants in developing countries. Seed treatment of chickpea demonstrated significantly (P = 0.05) greater seedling mortality in nonbacterized compared with bacterized seedlings. Bacterization significantly (P = 0.05) improved seed germination, plant height, number of pods/plant(-1), and seed dry weight.
DasGupta
S M
SM
Division of Plant-Microbe Interactions, National Botanical Research Institute, Rana Pratap Marg, Lucknow , 226 001, India.
Khan
N
N
Nautiyal
C S
CS
D000818:Animals; D001407:Bacillus / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D002417:Cattle; D005670:Fusarium / Q000378:metabolism; D008892:Milk / Q000382:microbiology*; D063245:Plant Development; D018517:Plant Roots / Q000382:microbiology; D010944:Plants / Q000382:microbiology*; D012639:Seeds / Q000254:growth & development; D012988:Soil Microbiology