Subgingival microbiota of periodontally untreated Mexican subjects with generalized aggressive periodontitis.
OBJECTIVE
Specific microbial profiles that may distinguish between generalized aggressive-periodontitis (GAgP) and generalized chronic-periodontitis (GCP) have, to date, not been described. The purpose of the present study was to describe the subgingival microbial composition of Mexican subjects with GAgP and compare it with that of individuals with GCP and periodontal health (PH).


METHODS
Seventy-seven subjects with GAgP (n=19), GCP (n=39) and PH (n=19) were selected. Clinical measurements included plaque accumulation, gingival erythema, bleeding on probing, suppuration, pocket depth and attachment level. Up to 28 subgingival plaque samples were obtained from each subject and analysed using the checkerboard DNA-DNA hybridization technique.


RESULTS
GAgP and GCP subjects harboured significantly higher levels and/or proportion of Porphyromonas gingivalis, Tannerella forsythia (levels: p<0.001, proportion: p<0.01), Prevotella nigrescens (p<0.05 levels) and "red" complex species (p<0.001 proportion) than PH subjects. All GAgP subjects were carriers of P. gingivalis and P. nigrescens. No significant differences in any of the 40 microbial species tested were detected between GAgP and GCP subjects.


CONCLUSIONS
Our results revealed that the microbial differences between GAgP and GCP subjects were only discrete and none of the bacterial species tested seemed to specifically differentiate the subgingival microbial profile of either periodontitis group.
Ximenez-Fyvie
Laurie Ann
LA
Laboratory of Molecular Genetics, School of Dentistry, National University of Mexico (UNAM), Mexico City 01760, Mexico. lximenez@post.harvard.edu
Almaguer-Flores
Argelia
A
Jacobo-Soto
Velia
V
Lara-Cordoba
Monica
M
Moreno-Borjas
Jazmin-Yunuen
JY
Alcantara-Maruri
Eulalio
E
D000190:Actinomyces / Q000145:classification / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D000368:Aged; D001439:Bacteroides / Q000145:classification / Q000302:isolation & purification; D002648:Child; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000145:classification / Q000382:microbiology*; D004890:Erythema / Q000382:microbiology; D005260:Female; D005881:Gingiva / Q000382:microbiology*; D005882:Gingival Diseases / Q000382:microbiology; D005884:Gingival Hemorrhage / Q000382:microbiology; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000382:microbiology; D010518:Periodontitis / Q000145:classification* / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D045242:Prevotella nigrescens / Q000302:isolation & purification; D013492:Suppuration