Penetration studies of topically applied substances: Optical determination of the amount of stratum corneum removed by tape stripping.
Tape stripping is a standard measuring method for the investigation of the dermatopharmacokinetics of topically applied substances using adhesive films. These tape strips are successively applied and removed from the skin after application and penetration of topically applied substances. Thus, layers of corneocytes and some amount of topical applied substances are removed. The amount of substances and the amount of stratum corneum removed with a single tape strip has to be determined for the calculation of the penetration profile. The topically applied substances removed from the skin can be determined by classical analytical methods like high-pressure liquid chromatography, mass spectroscopy, and spectroscopic measurements. The amount of corneocytes on the tape strips can be easily detected by their pseudoabsorption. In the present paper, an easy and cheap corneocyte density analyzer is presented that is based on a slide projector. Comparing the results of the measurements obtained by the corneocyte density analyzer and by uv-visible spectrometry, identical results were obtained.
Lademann
J
J
Charité-Universitätsmedizin Berlin, Department of Dermatology and Allergology, Center of Experimental and Applied Cutaneous Physiology (CCP), D-10098 Berlin, Germany. juergen.lademann@charite.de
Ilgevicius
A
A
Zurbau
O
O
Liess
H D
HD
Schanzer
S
S
Weigmann
H J
HJ
Antoniou
C
C
Pelchrzim
R V
RV
Sterry
W
W
D000287:Administration, Topical; D000328:Adult; D002452:Cell Count / Q000295:instrumentation* / Q000379:methods; D002990:Clobetasol / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism*; D055095:Optics and Photonics / Q000295:instrumentation; D010783:Photometry / Q000295:instrumentation / Q000379:methods; D012869:Skin Absorption / Q000502:physiology*; D053832:Surgical Tape
D002990:Clobetasol