title: [Kinetic properties of butyrylcholinesterases immobilised on pH-sensitive field-effect transistor surface and inhibitory action of steroidal glycoalkaloids on these enzymes].
abstract: The inhibitory action of steroid glycoalkaloids alpha-solanine, alpha-chaconine and tomatine on horse and human serum butyryl cholinesterases immobilized on the pH-sensitive field-effect transistors has been studied. Using acetyl- and butyryl choline as substrates, the optimal pH and the apparent kinetic parameters (< K(m) >, < V(max) >) of immobilized butyryl cholinesterases have been calculated in the absence of inhibitors. The affinity of each enzyme to glycoalkaloids has been estimated from calculation of apparent inhibition constants < K(i) > and inhibition coefficients i(0.5). Application of the studied cholinesterases for biosensoric determination of glycoalkaloids in the wide range of concentrations (10(-7)-10(-4) M) in different media has been discussed.
authors.forename: I V
authors.initials: IV
authors.lastname: Benilova
authors.forename: V M
authors.initials: VM
authors.lastname: Arkhypova
authors.forename: S V
authors.initials: SV
authors.lastname: Dziadevych
authors.forename: N
authors.initials: N
authors.lastname: Jaffrezic-Renault
authors.forename: C
authors.initials: C
authors.lastname: Martelet
authors.forename: O P
authors.initials: OP
authors.lastname: Soldatkin
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002091:Butyrylcholinesterase / Q000737:chemistry*; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012680:Sensitivity and Specificity; D012991:Solanaceous Alkaloids / Q000032:analysis* / Q000737:chemistry; D014173:Transistors, Electronic
chemical_list: D002800:Cholinesterase Inhibitors; D004800:Enzymes, Immobilized; D012991:Solanaceous Alkaloids; D002091:Butyrylcholinesterase