Antennally mediated negative feedback regulation of pheromone production in the pine engraver beetle, Ips pini.
Bark beetles use monoterpenoid aggregation pheromones to coordinate host colonization and mating. These chemical signals are produced de novo in midgut cells via the mevalonate pathway, and pheromone production may be regulated by a negative feedback system mediated through the antennae. In this study, we explored the effect of antennectomy on pheromone production and transcript levels of key mevalonate pathway genes in juvenile hormone III-treated male pine engraver beetles, Ips pini (Say). Antennectomized males produced significantly greater amounts of pheromone than podectomized males and those with intact antennae. Likewise, mRNA levels of three mevalonate pathway genes important in pheromone biosynthesis were measured by quantitative real-time PCR and found to be induced to a greater extent with antennectomy, suggesting a transcriptional regulation of pheromone production.
Ginzel
Matthew D
MD
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557, USA. ginzel@unr.edu
Bearfield
Jeremy C
JC
Keeling
Christopher I
CI
McCormack
Colin C
CC
Blomquist
Gary J
GJ
Tittiger
Claus
C
D000818:Animals; D001517:Coleoptera / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers; D005246:Feedback; D005786:Gene Expression Regulation; D010675:Pheromones / Q000502:physiology*; D028223:Pinus / Q000469:parasitology*; D018547:Plant Stems / Q000469:parasitology; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D010675:Pheromones