title: Chaperone protection of immature molybdoenzyme during molybdenum cofactor limitation.
abstract: Maturation of molybdoenzyme TorA involves chaperone TorD. This study shows that TorD is also required to protect apoTorA against proteolysis when the molybdenum cofactor is limiting in Escherichia coli. The absence of TorD leads to a complete loss of apoTorA during molybdenum cofactor deficiency whereas the presence of TorD maintains a significant amount of apoTorA that can be matured when the molybdenum cofactor becomes available.
authors.affiliation: Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Genest
authors.forename: Farida
authors.initials: F
authors.lastname: Seduk
authors.forename: Laurence
authors.initials: L
authors.lastname: Théraulaz
authors.forename: Vincent
authors.initials: V
authors.lastname: Méjean
authors.forename: Chantal
authors.initials: C
authors.lastname: Iobbi-Nivol
mesh_terms: D001051:Apoenzymes / Q000378:metabolism; D003067:Coenzymes / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000378:metabolism*; D008667:Metalloproteins / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism*; D000090006:Molybdenum Cofactors; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*; D011621:Pteridines / Q000378:metabolism*
chemical_list: D001051:Apoenzymes; D003067:Coenzymes; D029968:Escherichia coli Proteins; D008667:Metalloproteins; D018832:Molecular Chaperones; D000090006:Molybdenum Cofactors; D010089:Oxidoreductases, N-Demethylating; D011621:Pteridines; C088083:TorD protein, E coli; C022261:molybdopterin; C023186:trimethylamine dehydrogenase