Influence of static magnetic field on cadmium toxicity: study of oxidative stress and DNA damage in rat tissues.
In the present study, we investigated the effect of co-exposure to static magnetic field (SMF) and cadmium (Cd) on the biochemical parameters, antioxidant enzymes activity and DNA damage in rat tissues. Animals were treated with cadmium (CdCl(2), 40mg/L, per os) in drinking water during 4 weeks. Cd treatment induced an increase of plasma lactate dehydrogenase (LDH) and transaminases levels. Moreover, Cd treatment increased malondialdehyde (MDA) and 8-oxodGuo levels in rat tissues. However, the antioxidant enzymes activity such as the glutathione peroxidase (GPx), catalase (CAT) and the superoxide dismutase (SOD) were decreased in liver and kidney, while we noted a huge increase of hepatic and renal cadmium content. Interestingly, the combined effect of SMF (128mT, 1h/day during 30 consecutive days) and Cd (40mg/L, per os) decreased the GPx and CAT activities in liver compared to cadmium treated group. However, the association between SMF and Cd failed to alter transaminases, MDA and 8-oxodGuo concentration. Cd treatment altered antioxidant enzymes and DNA in liver and kidney of rats. Moreover, SMF associated to Cd disrupt this antioxidant response in liver compared to Cd-treated rats.
Amara
Salem
S
Laboratoire de Physiologie Intégrée Faculté des Sciences de Bizerte, 7021 Jarzouna, Tunisia.
Abdelmelek
Hafedh
H
Garrel
Catherine
C
Guiraud
Pascale
P
Douki
Thierry
T
Ravanat
Jean-Luc
JL
Favier
Alain
A
Sakly
Mohsen
M
Ben Rhouma
Khémais
K
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002104:Cadmium / Q000633:toxicity*; D002105:Cadmium Poisoning; D004249:DNA Damage; D008280:Magnetics; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D002104:Cadmium