title: Effect of the number of coats of simplified adhesive systems on microleakage of dentin-bordered composite restorations.
abstract: OBJECTIVE
  This study tested the null hypothesis that there is no difference on microleakage of dentin-bordered composite restorations using single or double coats of adhesive from one-bottle adhesive systems.
  
  
  METHODS
  The enamel surface was removed from freshly extracted bovine teeth, and standardized Class V cavities (3 x 3 x 1.5 mm) were made at the cervical areas of buccal surfaces. Teeth were restored and grouped according to type of adhesive systems [Prime Bond 2.1 (PB2.1), Prime & Bond NT (PBNT), and Single Bond (SB)] and to the number of coats (one or two) to be used. The restorations were polished and immersed in a 0.5% aqueous solution of basic fuchsin for four hours. Teeth were then sectioned and the most infiltrated section of each tooth was selected under magnification, scanned, and quantitatively analyzed using a computer program. Data were analyzed using two-way analysis of variance (ANOVA) and Student-Newman-Keuls (a=0.05).
  
  
  RESULTS
  Groups without filler content (PB2.1 and SB) showed no difference in microleakage using single or double coats. However, PBNT (with nanofiller) showed statistically less microleakage when only one coat was applied. The influence of the number of coats of the adhesive systems on dentin margin microleakage was material dependent.
  
  
  CONCLUSIONS
  All adhesive systems demonstrated microleakage, however, it could be minimized using two coats of non-filled or one coat of a filled adhesive system.
authors.affiliation: School of Dentistry of Bauru, University of São Paulo in Bauru, São Paulo, Brazil. sayuri_harada@yahoo.com
authors.forename: Tánia Sayuri
authors.initials: TS
authors.lastname: Harada
authors.forename: Flávia Bittencourt
authors.initials: FB
authors.lastname: Pazinatto
authors.forename: Linda
authors.initials: L
authors.lastname: Wang
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Atta
mesh_terms: D000096:Acetone; D000269:Adhesives; D000704:Analysis of Variance; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate; D002417:Cattle; D001840:Dental Bonding / Q000379:methods*; D003737:Dental Cavity Preparation; D003763:Dental Leakage / Q000517:prevention & control*; D003793:Dental Restoration, Permanent / Q000379:methods; D003806:Dentin Permeability; D017220:Dentin-Bonding Agents; D008422:Materials Testing; D011109:Polymethacrylic Acids; D011897:Random Allocation; D019279:Resin Cements; D018709:Statistics, Nonparametric; D019237:Tooth Cervix
chemical_list: D000269:Adhesives; D017220:Dentin-Bonding Agents; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D019279:Resin Cements; C444444:prime and bond 2.1; C115612:single bond; D000096:Acetone; D017438:Bisphenol A-Glycidyl Methacrylate