Adaptation of the rumen microbial population to native potato starch degradation determined with the gas production technique and the nylon bag technique.
Experiments were conducted to investigate the influence of the adaptation of rumen micro-organisms on the degradation of native potato starch (PS) in the rumen. Cows were fed with rations used for gas production (GP) analysis (dry cows, 1.6% starch) and for the nylon bag (NB) technique (lactating cows, 23% starch, mainly maize starch) and a ration containing 19% native PS (lactating cows). Fermentation characteristics of 13 samples were investigated with the GP technique using rumen fluid from cows fed each of the three rations. The same samples were investigated with the NB technique in the cows obtaining the NB ration and the PS ration. The results showed that the rate of GP was influenced by the source of the rumen fluid. The fermentation rate of PS was considerably enhanced by using rumen fluid adapted to the fermentation of native PS instead of using the other rumen fluids. Incubating in cows fed the PS ration, the rate of PS degradation determined with the NB technique, was higher compared with cows fed other rations. Using the PS ration the observed lag period for PS was shorter. The results show a clear influence of ration on the degradation characteristics of starch, determined with both the GP technique and the NB technique. However, these changes in behaviour did not explain observed differences in amounts of rumen escape PS measured in vivo in animal experiments and in situ, using the NB technique.
Cone
J W
JW
Animal Sciences Group, Wageningen UR, Lelystad, The Netherlands. john.cone@wur.nl
van Gelder
A H
AH
D000222:Adaptation, Physiological; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005285:Fermentation; D007774:Lactation / Q000187:drug effects / Q000378:metabolism*; D011897:Random Allocation; D012417:Rumen / Q000378:metabolism* / Q000382:microbiology*; D011198:Solanum tuberosum / Q000378:metabolism; D013213:Starch / Q000378:metabolism* / Q000494:pharmacology; D014908:Triticum / Q000378:metabolism; D014835:Volatilization; D003313:Zea mays / Q000378:metabolism
D013213:Starch