title: Human bone marrow: a reservoir for "enhanced effector memory" CD8+ T cells with potent recall function.
abstract: The role of human bone marrow (BM) CD8+ T cells in the immune response to viral Ags is poorly defined. We report here the identification and characterization of a functionally enhanced effector memory CD8+ T cell population (TEM) in the BM of patients undergoing total joint replacement for osteoarthritis. These BM-derived TEM differ strikingly from correlate cells in peripheral blood (PB), expressing elevated levels of CD27, HLA-DR, CD38, CD69, and unique patterns of chemokine receptors. Interestingly, while BM TEM have low levels of resting perforin and granzyme B, these molecules evidence profound up-regulation in response to TCR stimulation resulting in enhanced cytotoxic potential. Moreover, compared with the TEM subset in PB, BM CD8+ TEM cells demonstrate a more vigorous recall response to pooled viral Ags. Our results reveal that human BM serves as a repository for viral Ag-specific TEM with great therapeutic potential in vaccine development.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, University of Maryland School of Medicine, Baltimore, MD 21201-1619, USA.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Haidong
authors.initials: H
authors.lastname: Dong
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.forename: Stephen
authors.initials: S
authors.lastname: Voss
authors.forename: Lucinda
authors.initials: L
authors.lastname: Hinkley
authors.forename: Melissa
authors.initials: M
authors.lastname: Westergren
authors.forename: Guoliang
authors.initials: G
authors.lastname: Tian
authors.forename: Daniel
authors.initials: D
authors.lastname: Berry
authors.forename: David
authors.initials: D
authors.lastname: Lewellen
authors.forename: Richard G
authors.initials: RG
authors.lastname: Vile
authors.forename: Lieping
authors.initials: L
authors.lastname: Chen
authors.forename: Donna L
authors.initials: DL
authors.lastname: Farber
authors.forename: Scott E
authors.initials: SE
authors.lastname: Strome
mesh_terms: D000368:Aged; D000956:Antigens, Viral / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D003587:Cytomegalovirus / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D019707:Receptors, Chemokine / Q000096:biosynthesis
chemical_list: D000956:Antigens, Viral; D018984:Epitopes, T-Lymphocyte; D019707:Receptors, Chemokine