title: Quantitative PCR assays for mouse enteric flora reveal strain-dependent differences in composition that are influenced by the microenvironment.
abstract: The mammalian gastrointestinal (GI) tract is inhabited by over a hundred species of symbiotic bacteria. Differences among individuals in the composition of the GI flora may contribute to variation in in vivo experimental analyses and disease susceptibility. To investigate potential interindividual differences in GI flora composition, we developed real-time quantitative PCR-based assays for the detection of the eight members of the Altered Schaedler Flora (ASF) as representative members of different bacterial niches within the mammalian GI tract. Quantitative and reproducible strain-specific variations in the numbers of the ASF members were observed across 23 different barrier-housed inbred mouse strains, suggesting that the ASF assays can be used as sentinels for changes in GI flora composition. A significant cage effect was also detected. Isogenic mice that cohabited at weaning, whether from the same or different litters, showed little variation in ASF profiles. Conversely, litters split among different cages at weaning showed divergence in ASF profiles after three weeks. Individual ASF profiles, once established, were highly stable over time in the absence of environmental perturbation. Furthermore, cohabitation of different inbred strains maintained most of the interstrain variation in the GI flora, supporting a role of host genetics in determining GI flora composition.
authors.affiliation: Department of Genetics and Center for Gastrointestinal Biology and Disease, University of North Carolina, CB# 7264, Chapel Hill, North Carolina 27599, USA.
authors.forename: A
authors.initials: A
authors.lastname: Deloris Alexander
authors.forename: Roger P
authors.initials: RP
authors.lastname: Orcutt
authors.forename: Janell C
authors.initials: JC
authors.lastname: Henry
authors.forename: Joseph
authors.initials: J
authors.lastname: Baker
authors.forename: Anika C
authors.initials: AC
authors.lastname: Bissahoyo
authors.forename: David W
authors.initials: DW
authors.lastname: Threadgill
mesh_terms: D000818:Animals; D004777:Environment; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D005856:Germ-Free Life; D006799:Housing, Animal; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity