Purification, crystallization and preliminary X-ray analysis of the glucosamine-6-phosphate N-acetyltransferase from human liver.
Glucosamine-6-phosphate N-acetyltransferase from human liver, which catalyzes the transfer of an acetyl group from acetyl coenzyme A (AcCoA) to the primary amine of D-glucosamine 6-phosphate to form N-acetyl-D-glucosamine 6-phosphate, was expressed in a soluble form from Escherichia coli strain BL21 (DE3). The protein was purified to homogeneity using Ni(2+)-chelating chromatography followed by size-exclusion chromatography. Crystals of the protein were obtained by the hanging-drop vapour-diffusion method and diffracted to 2.6 A resolution. The crystals belonged to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 50.08, c = 142.88 A.
Wang
Juan
J
National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, People's Republic of China.
Zhou
Yan-Feng
YF
Li
Lan-Fen
LF
Liang
Yu-He
YH
Su
Xiao-Dong
XD
D000595:Amino Acid Sequence; D001483:Base Sequence; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D003460:Crystallization; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D051081:Glucosamine 6-Phosphate N-Acetyltransferase / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
D017931:DNA Primers; D010446:Peptide Fragments; D011994:Recombinant Proteins; D051081:Glucosamine 6-Phosphate N-Acetyltransferase