Concurrent administration of Neu2000 and lithium produces marked improvement of motor neuron survival, motor function, and mortality in a mouse model of amyotrophic lateral sclerosis.
The Fas pathway and oxidative stress mediate neuronal death in stroke and may contribute to neurodegenerative disease. We tested the hypothesis that these two factors synergistically produce spinal motor neuron degeneration in amyotrophic lateral sclerosis (ALS). Levels of reactive oxygen species were increased in motor neurons from ALS mice compared with wild-type mice at age 10 weeks, before symptom onset. The proapoptotic proteins Fas, Fas-associated death domain, caspase 8, and caspase 3 were also elevated. Oral administration of 2-hydroxy-5-(2,3,5,6-tetrafluoro-4-trifluoromethyl-benzylamino)-benzoic acid (Neu2000), a potent antioxidant, blocked the increase in reactive oxygen species but only slightly reduced activation of proapoptotic proteins. Administration of lithium carbonate (Li(+)), a mood stabilizer that prevents apoptosis, blocked the apoptosis machinery without preventing oxidative stress. Neu2000 or Li(+) alone significantly enhanced survival time and motor function and together had an additive effect. These findings provide evidence that jointly targeting oxidative stress and Fas-mediated apoptosis can prevent neuronal loss and motor dysfunction in ALS.
Shin
Jin Hee
JH
Department of Pharmacology, Ajou University School of Medicine, Sawon, Korea 442-749.
Cho
Sung Ig
SI
Lim
Hyang Ran
HR
Lee
Jae Keun
JK
Lee
Young Ae
YA
Noh
Jai Sung
JS
Joo
In Soo
IS
Kim
Kee-Won
KW
Gwag
Byoung Joo
BJ
D000690:Amyotrophic Lateral Sclerosis / Q000188:drug therapy* / Q000401:mortality; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D001565:Benzoates / Q000008:administration & dosage* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D005464:Fluorobenzenes; D016651:Lithium Carbonate / Q000008:administration & dosage* / Q000494:pharmacology; D051379:Mice; D009026:Mortality; D009043:Motor Activity / Q000187:drug effects; D009046:Motor Neurons / Q000187:drug effects / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D012459:Salicylates; D019014:fas Receptor / Q000378:metabolism; D062368:meta-Aminobenzoates
C519903:2-hydroxy-5-(2,3,5,6-tetrafluoro-4-trifluoromethyl-benzylamino)benzoic acid; D000975:Antioxidants; D001565:Benzoates; D005464:Fluorobenzenes; D012459:Salicylates; D019014:fas Receptor; D062368:meta-Aminobenzoates; D016651:Lithium Carbonate