Feeding strategies for E. coli fermentations demanding an enriched environment.
The addition of a carbon nutrient feed to a fed-batch cultivation is often not enough to obtain satisfactory growth and/or production. In some cases, an additional feed with for example supplementary amino acids or complex media is required. This work presents the development of feeding strategies where more than one feed is required and the knowledge of the growth requirements is low. Simulations and cultivations with E. coli are shown using the proposed feed controllers which are based on a probing control concept. The strategies work well and they can be used to shorten the process development phase considerably.
de Maré
L
L
Department of Automatic Control, LTH, Lund University, Box 118, 221 00 Lund, Sweden. lena.de_mare@control.lth.se
Cimander
C
C
Elfwing
A
A
Hagander
P
P
D003198:Computer Simulation; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D010101:Oxygen Consumption
D003470:Culture Media; D005947:Glucose