Pseudomonas stutzeri as a novel biocatalyst for pyruvate production from DL-lactate.
Pseudomonas stutzeri SDM oxidized DL-lactic acid (25.5 g l(-1)) into pyruvic acid (22.6 g l(-1)) over 24 h. Both NAD(+)-independent D-lactate dehydrogenase and NAD(+)-independent L-lactate dehydrogenase were found for the first time in the bioconversion of lactate to pyruvate based on the enzyme activity assay and proteomic analysis.
Hao
Jianrong
J
State Key Laboratory of Microbial Technology, Shandong University, Jinan, PR China. pingxu@sdu.edu.cn
Ma
Cuiqing
C
Gao
Chao
C
Qiu
Jianhua
J
Wang
Min
M
Zhang
Yinan
Y
Cui
Xing
X
Xu
Ping
P
D001711:Biotransformation; D019344:Lactic Acid / Q000378:metabolism*; D044223:Pseudomonas stutzeri / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism*
D019344:Lactic Acid; D019289:Pyruvic Acid