[The influence on the BK channel beta1 subunit expression of SO caused by high cholesterol in rabbits].
OBJECTIVE
To investigate the effects on protein expression of big-conductance Ca(2+)-sensitive K(+) channel (BKca) beta1 subunit caused by high cholesterol in Rabbit Oddi's sphincter (SO) cells.


METHODS
The rat-anti-rabbit polyclonal antiserum against beta1 subunits of BKca channel of SO cell was prepared. And the protein expression of BKca channel beta1 subunit of SO tissue was detected by semi-quantitative immunohistochemical staining.


RESULTS
The protein expression of BKca channels beta1 subunit of SO tissue in HC group was reduced, and there's statistically significant difference between the HC group and the control group.


CONCLUSIONS
High cholesterol can reduce the protein expression of BK Channel's beta1 subunit in Rabbits' SO which suggests high cholesterol can affect the function of BKca channel.
Du
Pang
P
Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. guojjj@fmmu.edu.cn
Wei
Jing-Guo
JG
Cui
Guang-Bin
GB
Wang
Ya-Rong
YR
Zhang
Xiao-Yong
XY
Ma
Ke-Jun
KJ
D000818:Animals; D002784:Cholesterol / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007106:Immune Sera / Q000032:analysis / Q000276:immunology; D051038:Large-Conductance Calcium-Activated Potassium Channel beta Subunits / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism; D009803:Sphincter of Oddi / Q000166:cytology / Q000378:metabolism*
D007106:Immune Sera; D051038:Large-Conductance Calcium-Activated Potassium Channel beta Subunits; D011993:Recombinant Fusion Proteins; D002784:Cholesterol