Ferrimonas futtsuensis sp. nov. and Ferrimonas kyonanensis sp. nov., selenate-reducing bacteria belonging to the Gammaproteobacteria isolated from Tokyo Bay.
Two novel mesophilic, facultatively anaerobic, selenate-reducing bacteria, designated strains FUT3661T and Asr22-7T, were isolated from a sediment sample and the alimentary tract of littleneck clams, respectively. Both sources of the samples were collected from the coast of Tokyo Bay, Japan. Cells were Gram-negative rods and motile by means of a polar flagellum. The strains reduced selenate to elemental selenium (Se0) and also reduced iron(III) oxyhydroxide, iron(III) citrate, arsenate, manganese(IV) oxide, elemental sulfur and oxygen and used lactate, pyruvate, yeast extract, tryptone and Casamino acids as electron donors and carbon sources. The strains contained both menaquinone (MK-7) and ubiquinones (Q-7 and Q-8) as isoprenoid quinones. The major fatty acids were C16:0 and C16:1omega9c. The G+C content of the genomic DNA was 58.1 mol% for strain FUT3661T and 57.2 mol% for strain Asr22-7T. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains were related to members of the genus Ferrimonas (<94.0% similarities), although the two novel strains formed a separate lineage. 16S rRNA gene sequence similarity between strains FUT3661T and Asr22-7T was 96%. On the basis of this polyphasic analysis, it was concluded that strains FUT3661T and Asr22-7T represent two novel species within the genus Ferrimonas, for which the names Ferrimonas futtsuensis sp. nov. (type strain FUT3661T=NBRC 101558T=DSM 18154T) and Ferrimonas kyonanensis sp. nov. (type strain Asr22-7T=NBRC 101286T=DSM 18153T) are proposed.
Nakagawa
Tatsunori
T
NITE Biological Resource Center, National Institute of Technology and Evaluation (NITE), Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
Iino
Takao
T
NITE Biological Resource Center, National Institute of Technology and Evaluation (NITE), Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
Suzuki
Ken-Ichiro
KI
NITE Biological Resource Center, National Institute of Technology and Evaluation (NITE), Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
Harayama
Shigeaki
S
NITE Biological Resource Center, National Institute of Technology and Evaluation (NITE), Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
D000596:Amino Acids / Q000378:metabolism; D000693:Anaerobiosis; D000818:Animals; D001149:Arsenates / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D049872:Bivalvia / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D005290:Ferric Compounds / Q000378:metabolism; D020563:Gammaproteobacteria / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D008345:Manganese / Q000378:metabolism; D008969:Molecular Sequence Data; D009068:Movement; D010084:Oxidation-Reduction; D010461:Peptones / Q000378:metabolism; D010802:Phylogeny; D011809:Quinones / Q000032:analysis / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D064586:Selenic Acid; D018036:Selenium Compounds / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism; D014041:Tokyo; D014871:Water Microbiology
D000596:Amino Acids; D001149:Arsenates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D005290:Ferric Compounds; D010461:Peptones; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D018036:Selenium Compounds; C017721:casamino acids; D008345:Manganese; D013455:Sulfur; C001830:tryptones; D002244:Carbon; D064586:Selenic Acid; C025657:arsenic acid