Urinary phytoestrogen excretion of rats bearing methylnitrosourea-induced mammary carcinoma in response to treatment with 2-methoxyestradiol.
The effect of treating mammary tumor-bearing rats with 2-methoxyestradiol (2-MeE2) on the urinary excretion of 12 phytoestrogens was investigated and compared with the changes in urinary excretion of estradiol metabolites. Alterations of excretion were registered for isoflavonoids, lignans and coumestans. However, due to large variations statistical significant differences were found only for two lignans, i.e. significant increases of enterodiol and matairesinol. Since the single components of phytoestrogens showed diverse alterations, excretions were expressed also by the ratio of total isoflavonoids to total lignans and compared with the estrogen ratios 2-hydroxyestrone to 16alpha-hydroxyestrone and A-ring to D-ring metabolites. The ratio of isoflavonoids to lignans was consistently decreased, whereas both ratios of estradiol metabolites were highly increased. The latter effect is probably due to demethylation of 2-methoxyestrone resulting in high catechol estrogen levels in urine. These results suggest that the high levels of catechol estrogens, produced by 2-MeE2 treatment, may have influenced the urinary excretion pattern of phytoestrogens.
Lippert
T H
TH
Section of Clinical Pharmacology, Department of Obstetrics and Gynecology, University of Tuebingen, Germany. endo.meno@med.uni-tuebingen.de
Adlercreutz
H
H
Berger
M R
MR
Seeger
H
H
Mueck
A O
AO
D000077584:2-Methoxyestradiol; D000818:Animals; D002277:Carcinoma / Q000139:chemically induced / Q000188:drug therapy* / Q000652:urine*; D003375:Coumestrol / Q000652:urine; D004958:Estradiol / Q000031:analogs & derivatives* / Q000627:therapeutic use; D005260:Female; D007529:Isoflavones / Q000652:urine; D017705:Lignans / Q000652:urine; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000652:urine*; D008770:Methylnitrosourea; D048789:Phytoestrogens / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley
D007529:Isoflavones; D017705:Lignans; D048789:Phytoestrogens; D004958:Estradiol; D008770:Methylnitrosourea; D000077584:2-Methoxyestradiol; D003375:Coumestrol