title: Comparison of hydroxylated print additives on antibody microarray performance.
abstract: Various hydroxylated additives were added to antibody print buffers at different concentrations to stabilize printed antibodies during normal array spot desiccation on commercial polymer-coated microarray slides. Polyvinyl alcohol addition to print buffers produced the most regular spot morphologies, homogeneous intra-spot antibody distribution, uniform fluorescence intensity, and improved analyte capture activity, maintained up to 1 month at 4 degrees C for capturing model analytes, anti-human IL-1beta, IL-4, and TNFalpha, on these microarraying slides.
authors.affiliation: Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523-1872, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.forename: David W
authors.initials: DW
authors.lastname: Grainger
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D019921:Biotinylation; D006801:Humans; D006900:Hydroxylation; D007091:Image Processing, Computer-Assisted; D053583:Interleukin-1beta; D015847:Interleukin-4 / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D011487:Protein Conformation; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000906:Antibodies; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4