title: Crystallization and initial crystallographic characterization of the Corynebacterium glutamicum nitrilotriacetate monooxygenase component A.
abstract: Safety and environmental concerns have recently dictated the proper disposal of nitrilotriacetate (NTA). Biodegradation of NTA is initiated by NTA monooxygenase, which is composed of two proteins: component A and component B. The NTA monooxygenase component A protein from Corynebacterium glutamicum was crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium sulfate as the precipitant. X-ray diffraction data were collected to a maximum resolution of 2.5 A on a synchrotron beamline. The crystal belongs to the monoclinic space group C2, with unit-cell parameters a = 111.04, b = 98.51, c = 171.61 A, beta = 101.94 degrees . The asymmetric unit consists of four molecules, corresponding to a packing density of 2.3 A(3) Da(-1). The structure was solved by molecular replacement. Structure refinement is in progress.
authors.affiliation: Beamline Division, Pohang Accelerator Laboratory, Pohang, Kyungbuk 790-784, South Korea. kkj@postech.ac.kr
authors.forename: Kyung-Jin
authors.initials: KJ
authors.lastname: Kim
authors.forename: Sujin
authors.initials: S
authors.lastname: Kim
authors.forename: Sujin
authors.initials: S
authors.lastname: Lee
authors.forename: Beom Sik
authors.initials: BS
authors.lastname: Kang
authors.forename: Heung-Soo
authors.initials: HS
authors.lastname: Lee
authors.forename: Tae-Kwang
authors.initials: TK
authors.lastname: Oh
authors.forename: Myung Hee
authors.initials: MH
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D002850:Chromatography, Gel; D003352:Corynebacterium / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D021122:Protein Subunits / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D021122:Protein Subunits; D006899:Mixed Function Oxygenases; C072680:nitrilotriacetate monooxygenase