title: Isolation and characterization of a fish F-type lectin from gilt head bream (Sparus aurata) serum.
abstract: A novel fucose-binding lectin, designated SauFBP32, was purified by affinity chromatography on fucose-agarose, from the serum of the gilt head bream Sparus aurata. Electrophoretic mobility of the subunit revealed apparent molecular weights of 35 and 30 kDa under reducing and non-reducing conditions, respectively. Size exclusion analysis suggests that the native lectin is a monomer under the selected experimental conditions. Agglutinating activity towards rabbit erythrocytes was not significantly modified by addition of calcium or EDTA; activity was optimal at 37 degrees C, retained partial activity by treatment at 70 degrees C, and was fully inactivated at 90 degrees C. On western blot analysis, SauFBP showed intense cross-reactivity with antibodies specific for a sea bass (Dicentrarchus labrax) fucose-binding lectin. In addition, the similarity of the N-terminal sequence and a partial coding domain to teleost F-type lectins suggests that SauFBP32 is a member of this emerging family of lectins.
authors.affiliation: Marine Immunobiology Laboratory, Department of Animal Biology, University of Palermo, Via Archirafi 18, Palermo, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Cammarata
authors.forename: Gigliola
authors.initials: G
authors.lastname: Benenati
authors.forename: Eric W
authors.initials: EW
authors.lastname: Odom
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Salerno
authors.forename: Aiti
authors.initials: A
authors.lastname: Vizzini
authors.forename: Gerardo R
authors.initials: GR
authors.lastname: Vasta
authors.forename: Nicolò
authors.initials: N
authors.lastname: Parrinello
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D037102:Lectins / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D021541:Sea Bream; D017386:Sequence Homology, Amino Acid
chemical_list: D037102:Lectins