Depletion of T(reg) cells inhibits minimal residual disease after surgery of HPV16-associated tumours.
It is generally accepted that T regulatory cells (T(reg) CD4(+)CD25(+)Foxp3(+)) play an important role in the suppression of tumour immunity. We examined the impact of T(reg) cell depletion with anti-CD25 antibody as adjuvant therapy in the treatment of minimal residual disease after excision of murine HPV16-associated tumours. We found that the depletion of T(reg) cells inhibited growth of the recurrences after surgery of HPV16-associated MHC class I+ as well as MHC class I-deficient tumours transplanted in syngeneic mice. These results demonstrate that depletion of CD25(+)CD4(+) T(reg) cells can be used as an efficient adjuvant treatment improving the results of surgery in the experimental systems mimicking human MHC class I+ and MHC class I-deficient, HPV16-associated neoplasms. Therefore, this therapeutic modality is worth being examined in patients with minimal residual HPV16-associated tumour disease after surgery.
Símová
Jana
J
Institute of Molecular Genetics Academy of Sciences of the Czech Republic, CZ-166 37 Prague 6, Czech Republic.
Bubeník
Jan
J
Bieblová
Jana
J
Rosalia
Rodney Alexander
RA
Fric
Jan
J
Reinis
Milan
M
D000818:Animals; D003131:Combined Modality Therapy; D005805:Genes, MHC Class I / Q000235:genetics / Q000276:immunology; D052162:Human papillomavirus 16 / Q000302:isolation & purification*; D007167:Immunotherapy / Q000379:methods*; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy* / Q000821:virology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018365:Neoplasm, Residual; D030361:Papillomavirus Infections / Q000150:complications / Q000276:immunology* / Q000821:virology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D053645:Interleukin-2 Receptor alpha Subunit