title: [Development of diagnostic kit "Ptakh-Grip PLR" for detection of highly pathogenic strain of avian influenza virus H5N1 by polymerase chain reaction].
abstract: A diagnostic kit for detection of avian influenza virus by real-time polymerase chain reaction was developed. This kit allows to identify the influenza viruses type A and highly pathogenic strain of avian influenza virus H5N1. The diagnostic kit is universal and adopted for ABI PRISM SDS (Applied Biosystems), RotorGene (Corbett Research) and iQCycler (BioRad) PCR machines.
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Bisiuk
authors.forename: S D
authors.initials: SD
authors.lastname: Mel'nichuk
authors.forename: R V
authors.initials: RV
authors.lastname: Oblap
authors.forename: V G
authors.initials: VG
authors.lastname: Spiridonov
authors.forename: D L
authors.initials: DL
authors.lastname: Martynenko
authors.forename: M D
authors.initials: MD
authors.lastname: Mel'nichuk
authors.forename: V T
authors.initials: VT
authors.lastname: Lisovenko
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001717:Birds; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D005585:Influenza in Birds / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014774:Virulence
chemical_list: D012367:RNA, Viral