title: [Fluorescein angiography of retinal neovascularization model in mice].
abstract: OBJECTIVE
  To investigate a high molecular weight fluorescein angiography in the application of retinal neovascularization model in mouse.
  
  
  METHODS
  retinal neovascularization model was induced by exposure mouse to an environment containing high concentration of oxygen. High molecular weight fluorescein isothiocyanate dextran were perfused through the left ventricle directly, then the mouse eyes were enucleated and fixed with 4% paraformaldehyde. The retina was separated from the eyecup and flat mounting was performed on a gelatin coated slide. The vasculature was examined under fluorescent microscope.
  
  
  RESULTS
  The whole retinal vasculature was clearly visualized under fluorescent microscope. By focusing on different layer of the tissue, superficial, deep vascular plexus and connecting vessels also could be distinguished. The neovascular response occurred at the junction between the vascular and the avascular retina.
  
  
  CONCLUSIONS
  High molecular weight fluorescein angiography can be applied for retinal neovascularization evaluation.
authors.affiliation: Tianjin Medical University, Tianjin Eye Hospital, Tianjin 300022, China.
authors.forename: Yi-chun
authors.initials: YC
authors.lastname: Kong
authors.forename: Mei
authors.initials: M
authors.lastname: Han
authors.forename: Kan-xing
authors.initials: KX
authors.lastname: Zhao
authors.forename: Ning-dong
authors.initials: ND
authors.lastname: Li
authors.forename: Jin-yong
authors.initials: JY
authors.lastname: Lin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D019793:Fluorescein; D005451:Fluorescein Angiography / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000633:toxicity; D015861:Retinal Neovascularization / Q000175:diagnosis*
chemical_list: D010100:Oxygen; D019793:Fluorescein