title: Mouse transthyretin-related protein is a hydrolase which degrades 5-hydroxyisourate, the end product of the uricase reaction.
abstract: Uric acid is the end product of the purine degradation pathway in humans. It is catabolized to allantoin by urate oxidase or uricase (E.C. 1.7.3.3.) in most vertebrates except humans, some primates, birds, and certain species of reptiles. Here we provide evidence that mouse transthyretin-related protein facilitates the hydrolysis of 5-hydroxyisourate, the end product of the uricase reaction. Mutagenesis experiments showed that the residues that are absolutely conserved across the TRP family, including His11, Arg51, His102, and the C-terminal Tyr-Arg-Gly-Ser, may constitute the active site of mTRP. Based on these results, we propose that the transthyretin-related proteins present in diverse organisms are not functionally related to transthyretin but actually function as hydroxyisourate hydrolases.
authors.affiliation: Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Korea.
authors.forename: Youra
authors.initials: Y
authors.lastname: Lee
authors.forename: Byoung Chul
authors.initials: BC
authors.lastname: Park
authors.forename: Do Hee
authors.initials: DH
authors.lastname: Lee
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
authors.forename: Sayeon
authors.initials: S
authors.lastname: Cho
authors.forename: Choong Hwan
authors.initials: CH
authors.lastname: Lee
authors.forename: Jong Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Pyung Keun
authors.initials: PK
authors.lastname: Myung
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Park
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D006867:Hydrolases / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016415:Sequence Alignment; D014503:Urate Oxidase / Q000378:metabolism*; D014527:Uric Acid / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: C510200:5-hydroxyisourate; D014527:Uric Acid; D014503:Urate Oxidase; D006867:Hydrolases; D000581:Amidohydrolases; C515665:transthyretin-related protein, mouse