title: Single-molecule biology: what is it and how does it work?
abstract: Biochemistry and structural biology are undergoing a dramatic revolution. Until now, we have tried to study subtle and complex biological processes by crude in vitro techniques, looking at average behaviors of vast numbers of molecules under conditions usually remote from those existing in the cell. Researchers have realized the limitations of this approach, but none other has been available. Now, we can not only observe the nuances of the behaviors of individual molecules but prod and probe them as well. Perhaps most important is the emerging ability to carry out such observations and manipulations within the living cell. The long-awaited leap to an in vivo biochemistry is at last underway.
authors.affiliation: Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071, USA. jordanka@uwyo.edu
authors.forename: Jordanka
authors.initials: J
authors.lastname: Zlatanova
authors.forename: Kensal
authors.initials: K
authors.lastname: van Holde
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D006801:Humans; D046911:Macromolecular Substances / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D013816:Thermodynamics
chemical_list: D046911:Macromolecular Substances