title: Establishment of a high sensitivity plasma assay for human pentraxin3 as a marker for unstable angina pectoris.
abstract: OBJECTIVE
  Plasma pentraxin 3 (PTX3) levels are increased in patients with acute myocardial infarction, yet its involvement in unstable angina pectoris (UAP) remains unclear. To critically evaluate the role of PTX3 in UAP, a sensitive and precise measurement of PTX3 concentration is needed.
  
  
  RESULTS
  We established a high sensitive plasma ELISA assay system for the detection of PTX3 using monoclonal antibodies. The lower limit of detection of our ELISA was 0.1 ng/mL, sensitivity far greater than the current commercially available kit. Plasma samples were obtained from 162 consecutive patients treated for hypertension, hyperlipidemia, diabetes mellitus, or cardiovascular disease at a physician's office. PTX3 was not associated with any known coronary risk factors. Additionally, we collected plasma samples from 252 consecutive subjects admitted to a university hospital for coronary artery assessment by coronary angiography. PTX3 was significantly increased in patients in whom coronary intervention was performed. We further analyzed the plasma level of PTX3 in 52 patients with effort angina (EAP) and 16 patients with UAP. Compared with the control group, PTX3 were significantly higher in the UAP group.
  
  
  CONCLUSIONS
  The levels of plasma PTX3 were increased in patients with arterial inflammation, especially UAP. This PTX3 detection system will be useful for the prediction of UAP.
authors.affiliation: Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1, Komaba, Meguro, Tokyo, 153-8904, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Inoue
authors.forename: Akira
authors.initials: A
authors.lastname: Sugiyama
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Reid
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ito
authors.forename: Katsumi
authors.initials: K
authors.lastname: Miyauchi
authors.forename: Sei
authors.initials: S
authors.lastname: Mukai
authors.forename: Mina
authors.initials: M
authors.lastname: Sagara
authors.forename: Kyoko
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Satoh
authors.forename: Isao
authors.initials: I
authors.lastname: Kohno
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kurata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ota
authors.forename: Alberto
authors.initials: A
authors.lastname: Mantovani
authors.forename: Takao
authors.initials: T
authors.lastname: Hamakubo
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Daida
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kodama
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000789:Angina, Unstable / Q000097:blood* / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism*; D003324:Coronary Artery Disease / Q000097:blood / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012307:Risk Factors; D012680:Sensitivity and Specificity; D000683:Serum Amyloid P-Component / Q000235:genetics / Q000378:metabolism*; D000099153:Pentraxins
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein; D000683:Serum Amyloid P-Component; D000099153:Pentraxins