title: Immunohistochemical MHC-II and interleukin 2-R (CD25) expression in lymph nodes of pigs with spontaneous postweaning multisystemic wasting syndrome (PMWS).
abstract: Immunohistochemical expression of immunocompetent cells bearing major histocompatibility complex (MHC-II) and interleukin 2-R (IL2-R) (CD25) molecules was performed on lymph nodes with spontaneous postweaning multisystemic wasting syndrome (PMWS). Control lymph nodes displayed intense diffuse immunoreactivity to MHC-II in both follicles and interfollicular areas. A marked reduction of follicular MHC-II immunoreactivity and inconsistent staining of histiocytes in interfollicular areas was observed in PMWS cases with a slight lymphoid depletion; in those cases with moderate to severe lymphoid depletion, there was a progressive decrease in MHC-II expression. In controls and in slightly depleted nodes, IL2-R was equally expressed in interfollicular tissue and in follicles, whereas in moderate and severe cases, it was detected in interfollicular remnants only. Immunohistochemical staining was scored semiquantitatively. The mean MHC-II score was significantly reduced in PMWS cases compared with controls (Spearman test), whereas there was no difference in the IL2-R score. The evident reduction of MHC-II immunoreactivity suggests an impairment in MHC-II linked antigen presenting cell expression.
authors.affiliation: Section of General Pathology and Anatomic Pathology, Via Tolara di Sopra, 50, 40064 Ozzano Emilia, Bologna, Italy. lmandrioli@vet.unibo.it
authors.forename: L
authors.initials: L
authors.lastname: Mandrioli
authors.forename: G
authors.initials: G
authors.lastname: Sarli
authors.forename: M
authors.initials: M
authors.lastname: Zengarini
authors.forename: S
authors.initials: S
authors.lastname: Panarese
authors.forename: P S
authors.initials: PS
authors.lastname: Marcato
mesh_terms: D000818:Animals; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D007150:Immunohistochemistry; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D008198:Lymph Nodes / Q000378:metabolism* / Q000473:pathology; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000378:metabolism*; D013552:Swine
chemical_list: D000949:Histocompatibility Antigens Class II; D053645:Interleukin-2 Receptor alpha Subunit