[Effect of ligustrazine, synthetic borneol on lasting time of ciliary movement].
OBJECTIVE
To study the using of the method in vitro and in vivo of lasting time of ciliary movement in ligustrazine, synthetic borneol.


METHODS
(1) Method in vitro-The lasting time of ciliary movement was observed with forty-fold optical microscope after dropping liquid medicine on exteacorporeal frog palate mucosa. Then mucosa was cleared. It was observed whether the ciliary movement was recovered and the lasting time was recorded from recovering to stopping once again. (2) Method in vivo-Liquid medicine or normal saline was dropped on frog palate mucosa by contacting thirty minutes. Then mucosa was cleared. The lasting time of ciliary movement was observed with forty-fold optical microscope by separating palate mucosa.


RESULTS
Relatively percentage of lasting time of ciliary movement of ligustrazine in vitro and in vivo was 9.8%, 87.3%; The relatively percentage of synthetic borneol in vitro and in vivo was 9.3%, 89.5%.


CONCLUSIONS
The method in vitro and in vivo of lasting time of ciliary movement can be the one of the selecting ways of Chinese drug's toxicity of nasal mucosa, and it have virtues and defects respectively. Ligustrazine, synthetic borneol have significantly toxical effect on exteacorporeal lasting time of ciliary movement.
Pan
Jia
J
Department of Pharmacology, Sicuan Institute of Chinese Material Medica, Chengdu 610041, China. panjia028@163.com
Zou
Wen-Jun
WJ
Liu
Jie
J
Zhang
Bai-Jia
BJ
Liu
Ya-Ou
YO
Pan
Xiao-Juan
XJ
D000281:Administration, Intranasal; D000818:Animals; D001892:Camphanes / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D002465:Cell Movement / Q000187:drug effects*; D002923:Cilia / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity; D005260:Female; D008297:Male; D009297:Nasal Mucosa / Q000187:drug effects*; D011719:Pyrazines / Q000494:pharmacology* / Q000633:toxicity; D011893:Rana esculenta; D013995:Time
D001892:Camphanes; D004365:Drugs, Chinese Herbal; D011719:Pyrazines; C022871:isoborneol; C017953:tetramethylpyrazine