[Expressions of Bcl-2 and Caspase-3 in the internal organs of rats when tetramine was administered].
OBJECTIVE
To find whether Bcl-2 and Caspase-3 take part in the pathophysiological mechanism of tetramine toxification.


METHODS
Sixty Sprague-Dawley rats were divided into normal control group, the sham poisoned group, high dose poisoned group, low dose poisoned group. High dose poisoned group were administered 1.0 mg/kg weight body tetramine by mouth, however low dose poisoned group was administered tetramine 0.1 mg/kg weight body by mouth. The rats of the sham poisoned group were administered water, and rats of normal control group were given nothing. Bcl-2 and Caspase-3 were detected by immunohistochemistry staining and the results were assessed by image analysis system.


RESULTS
The expressions of Bcl-2 and Caspase-3 in all organs were similar, ie, Bcl-2 and Caspase-3 expressed obviously in all organs of high dose poisoned group; in all organs of low dose poisoned group, they were hardly detected at 30 min after administration, however, at 3 h after administration, they could be detected obviously; Bcl-2 got to peak at 6 h-3 d after administration and Caspase-3 got to peak at 24 h-3 d after administration.


CONCLUSIONS
Bcl-2 and Caspase-3 take part in the pathophysiological procedure of tetramine poisoned rats.
Wang
Ye
Y
West China Preclinical and Forensic Medicine School, Sichuan University, Chengdu 610041, China. wangyh11@163.com
Shen
Li-Hua
LH
Chen
Wei-Jie
WJ
Liu
Min
M
Li
Fan
F
Liao
Zhi-Gang
ZG
D000818:Animals; D001952:Bridged-Ring Compounds / Q000008:administration & dosage / Q000506:poisoning*; D053148:Caspase 3 / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
D001952:Bridged-Ring Compounds; D019253:Proto-Oncogene Proteins c-bcl-2; C505410:Casp3 protein, rat; D053148:Caspase 3; C010349:tetramethylenedisulfotetramine