title: Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering.
abstract: The aim of this study was to produce a natural, acellular matrix from porcine bladder tissue for use as a scaffold in developing a tissue-engineered bladder replacement. Full-thickness, intact porcine bladders were decellularised by distention and immersion in hypotonic buffer containing 0.1% (w/v) SDS and nuclease enzymes. Histological analysis of the resultant matrices showed they were completely acellular; that the major structural proteins had been retained and that there were some residual poorly soluble intracellular proteins. The amount of DNA per mg dry weight of fresh porcine bladder was 2.8 (+/-0.1) microg/mg compared to 0.1 (+/-0.1) microg/mg in decellularised bladder and biochemical analysis showed proportional differences in the hydroxyproline and glycosaminoglycan content of the tissue before and after decellularisation. Uniaxial tensile testing indicated that decellularisation did not significantly compromise the ultimate tensile strength of the tissue. There was, however, an increase in the collagen and elastin phase slopes indicating decreased extensibility. Cytotoxicity assays using porcine smooth muscle cell cultures excluded the presence of soluble toxins in the biomaterial. In summary, a full-thickness natural acellular matrix retaining the major structural components and strength of the urinary bladder has been successfully developed. The matrix is biocompatible with bladder-derived cells and has potential for use in urological surgery and tissue-engineering applications.
authors.affiliation: Jack Birch Unit of Molecular Carcinogenesis, Department of Biology, University of York, Heslington, York YO10 5YW, UK.
authors.forename: Fiona
authors.initials: F
authors.lastname: Bolland
authors.forename: Sotiris
authors.initials: S
authors.lastname: Korossis
authors.forename: Stacy-Paul
authors.initials: SP
authors.lastname: Wilshaw
authors.forename: Eileen
authors.initials: E
authors.lastname: Ingham
authors.forename: John
authors.initials: J
authors.lastname: Fisher
authors.forename: John N
authors.initials: JN
authors.lastname: Kearney
authors.forename: Jennifer
authors.initials: J
authors.lastname: Southgate
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001705:Bioprosthesis; D002474:Cell-Free System / Q000637:transplantation; D005109:Extracellular Matrix / Q000502:physiology* / Q000637:transplantation; D008297:Male; D008422:Materials Testing; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D001743:Urinary Bladder / Q000737:chemistry* / Q000166:cytology / Q000502:physiology* / Q000637:transplantation
chemical_list: D001672:Biocompatible Materials