Identification of differentially expressed genes induced by ozone stress in sensitive and tolerant poplar hybrids.
The differential expression of genes induced by an acute ozone treatment was analysed in two poplar clones, i.e. Populus deltoides x maximowiczii, Eridano clone, and Populus x euoramericana, I-214 clone, respectively, sensitive and tolerant to this pollutant, performing suppression subtractive hybridization (SSH). From the obtained cDNA libraries several clones were obtained, which corresponded to differentially regulated genes. Preliminary expression analyses of four genes, Fs23A-LRP, Ft33B-CaBP, Ft312B-WRKY, and Ft32C-WAK identified by the primary screening, were conducted by semi-quantitative RT-PCR to evaluate the ozone responsiveness of the libraries. The most interesting finding is the co-activation of a wall associated kinase and a WRKY transcription factor in response to O(3) stress.
Rizzo
Milena
M
Dipartimento di Biologia delle Piante Agrarie, Sezione di Genetica, Università di Pisa, Via Matteotti 1/B Pisa, Italy. mrizzo@agr.unipi.it
Bernardi
Rodolfo
R
Salvini
Mariangela
M
Nali
Cristina
C
Lorenzini
Giacomo
G
Durante
Mauro
M
D000393:Air Pollutants / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015723:Gene Library; D006824:Hybridization, Genetic; D009693:Nucleic Acid Hybridization; D010126:Ozone / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics*; D032107:Populus / Q000187:drug effects / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity
D000393:Air Pollutants; D010940:Plant Proteins; D010126:Ozone