title: Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis.
abstract: Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. narisara@tropmedres.ac
authors.forename: Narisara
authors.initials: N
authors.lastname: Chantratita
authors.forename: Vanaporn
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: Aunchalee
authors.initials: A
authors.lastname: Thanwisai
authors.forename: Direk
authors.initials: D
authors.lastname: Limmathurotsakul
authors.forename: Allen C
authors.initials: AC
authors.lastname: Cheng
authors.forename: Wirongrong
authors.initials: W
authors.lastname: Chierakul
authors.forename: Nicholas P J
authors.initials: NP
authors.lastname: Day
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Peacock
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D016957:Burkholderia pseudomallei / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests; D008554:Melioidosis / Q000175:diagnosis* / Q000453:epidemiology; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial