title: A fully electronic sensor for the measurement of cDNA hybridization kinetics.
abstract: Ion sensitive field effect transistors (ISFET) are candidates for a new generation of fully electrical DNA sensors. To this purpose, we have modified ISFET sensors by adsorbing on their Si(3)N(4) surface poly-L-lysine and single (as well as double) stranded DNA. Once coupled to an accurate model of the oppositely charged layers adsorbed on the surface, the proposed sensor allows quantitatively evaluating the adsorbed molecules densities, as well as estimating DNA hybridization kinetics.
authors.affiliation: DEI, Department of Information Engineering, Padova University, Italy. leonardo.bandiera@gmail.com
authors.forename: L
authors.initials: L
authors.lastname: Bandiera
authors.forename: G
authors.initials: G
authors.lastname: Cellere
authors.forename: S
authors.initials: S
authors.lastname: Cagnin
authors.forename: A
authors.initials: A
authors.lastname: De Toni
authors.forename: E
authors.initials: E
authors.lastname: Zanoni
authors.forename: G
authors.initials: G
authors.lastname: Lanfranchi
authors.forename: L
authors.initials: L
authors.lastname: Lorenzelli
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D018076:DNA, Complementary / Q000032:analysis* / Q000378:metabolism; D007700:Kinetics; D009693:Nucleic Acid Hybridization
chemical_list: D018076:DNA, Complementary