[Protective effects of Xuebijing injection on kidney in rats with sepsis].
OBJECTIVE
To investigate the protective effects of the integrated traditional Chinese and Western medicine Xuebijing injection on the kidney in rats with sepsis.


METHODS
Sepsis model was reproduced in rats with cecal ligation and puncture (CLP). Eighty healthy Wistar rats were randomly divided into four groups: control group, sham-operation group, CLP model group, and Xuebijing group. The two latter groups were divided into four groups of 2, 8, 24, 48-hour subgroups with 8 rats in each subgroup. Serum levels of creatinine (Cr), blood urea nitrogen (BUN), Na(+), K(+) were measured. beta (2)-microglobulin (beta (2)-MG) was determined by radioimmunoassay, urine level of Cr, Na(+), interleukin-6 (IL-6) levels in renal tissue, and fractional excretion of sodium (FENa) were determined by enzyme linked immunoadsorbent assay (ELISA). Light microscopy was used to assess the pathological changes in kidney.


RESULTS
Compared with CLP model group,the level of beta (2)-MG was significantly decreased at 2 hours in Xuebijing group (P<0.01). Similarly, serum BUN content, renal IL-6 level were lowered at 8 hours, and serum contents of BUN as well as Cr were also decreased in Xuebijing group than those in model group at 48 hours after CLP (P<0.05 or P<0.01).


CONCLUSIONS
Xuebijing injection possesses the protective effects on the kidney in rats with sepsis, which may be related with the synthesis and release of IL-6.
Zhu
Xue-qi
XQ
Department of Emergency, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China.
Wang
Lei
L
Liu
Qing-quan
QQ
Yao
Yong-ming
YM
D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D015850:Interleukin-6 / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000188:drug therapy* / Q000473:pathology
D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; C549376:Xuebijing