[Pharmacokinetics and tissue distribution of atractylenolide III in rats].
OBJECTIVE
[corrected] To establish an HPLC method for the analysis of pharmacokinetics and tissue distribution of atractylenolide III in rats.


METHODS
The biological samples were extracted with ether. The chromatographic conditions were as follows: Hypersil ODS column (150 mm x 4.6 mm, 5 microm) was used. The mobile phase was methnol/warter (67 : 33) with a flow rate of 1.0 ml/min under the column temperature of 25 degrees C, and the detection wavelength was set at 220 nm.


RESULTS
The recovery of the method was 85.12% (RSD = 5.57%). The linear range was 0.2 microg/ml - 18.5 microg/ml (r = 0.9996) in rat plasma. The Lowest Limit of detection was 0.10 microg/ ml (S/N > 3). The within-day and between-day precision were from 0.98% to 6.19% and 12.95% to 15.48%, respectively. After oral administration of atractylenolide III (100 mg/kg), the concentration-time profiles of atractylenonlide III fit a two compartment model. In main effect tissues, the atractylenolide III concentration was followed as in order C(lung) > C(cerebellum) > C(heart) > C(cerebrum), and that was C(spleen) > C(liver) > C(kidney) in eliminated tissues.


CONCLUSIONS
The method is accurate, stable and reliable, and can be used for the investigation of atractylenolide III in plasma and tissues of rats.
Li
Cui-Qin
CQ
School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
He
Lang-Chong
LC
Deng
Ting
T
D000818:Animals; D019540:Area Under Curve; D031174:Atractylodes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000493:pharmacokinetics; D005260:Female; D007783:Lactones / Q000302:isolation & purification / Q000493:pharmacokinetics*; D008168:Lung / Q000378:metabolism; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000493:pharmacokinetics*; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution
D004365:Drugs, Chinese Herbal; D007783:Lactones; D012717:Sesquiterpenes; C424802:atractylenolide III