Generation of transgenic mice for cardiovascular research.
The transgenic mouse technology is a powerful tool that can be used for creating animal models for cardiovascular disease to identify molecular pathogenic mechanisms and for identifying the physiological functions of a novel gene. A transgenic animal can be generated by several methods, which include microinjection of a DNA fragment into the pronucleus, embryonic stem cell manipulation and injection, sperm-mediated transgenesis, and viral infection of preimplanted embryos. The microinjection method is one of the most widely used approaches. This method involves four steps: (1) collection of fertilized eggs from the superovulated female, (2) injection of DNA into the pronucleus of fertilized eggs, (3) transfer of the injected eggs back into the oviduct of a pseudopregnant foster recipient, allowing the eggs to develop into pups, and (4) identification of the transgenic founder and establishment of transgenic lines through further breeding.
Tian
Xiao-Li
XL
Department of Molecular Cardiology, The Cleveland Clinic Foundation, OH, USA.
Wang
Qing K
QK
D000818:Animals; D001706:Biopsy; D002318:Cardiovascular Diseases / Q000235:genetics; D004247:DNA / Q000302:isolation & purification; D004195:Disease Models, Animal; D018628:Gene Dosage; D015870:Gene Expression; D017930:Genes, Reporter; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections; D016372:RNA, Antisense; D013480:Superovulation; D019076:Transgenes; D015053:Zygote
D016372:RNA, Antisense; D004247:DNA