Animal models for disease: knockout, knock-in, and conditional mutant mice.
Diseases with a genetic basis can be modeled with knockout, knock-in, and conditional mutant gene-targeted mice. In the following, we provide detailed protocols for gene targeting. Gene targeting of embryonic stem cells can be accomplished by laboratories equipped for tissue culture. Alternatively, many gene-targeting services divide the work of targeting with a customer lab. In this collaborative situation, knowledge of the entire process helps ensure a successful outcome. The construction of chimeras for germ-line transmission is not described here, because this procedure is beyond the means of most laboratories, typically is provided by transgenic core facilities, and is best learned through hands-on demonstration.
LePage
David F
DF
Department of Genetics, Case Western Reserve University, Cleveland, OH, USA.
Conlon
Ronald A
RA
D000818:Animals; D015139:Blotting, Southern; D002318:Cardiovascular Diseases / Q000235:genetics; D033481:Chromosomes, Mammalian; D004247:DNA / Q000302:isolation & purification; D004195:Disease Models, Animal; D053595:Embryonic Stem Cells; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors; D019426:Integrases; D051379:Mice / Q000235:genetics*; D018345:Mice, Knockout; D014162:Transfection / Q000379:methods
D004247:DNA; C045073:Cre recombinase; D019426:Integrases