Kinetic parameters and thermodynamic values of beta-xylosidase production by Kluyveromyces marxianus.
Kinetic parameters for production of beta-xylosidase by Kluyveromyces marxianus were determined in growth media containing glucose, xylose, cellobiose, sucrose and lactose as carbon sources. K. marxianus achieved maximum beta-xylosidase specific product yield (Y(P/X)) when grown on xylose. Basal level of activity was achieved in cultures grown on glucose. Kinetic parameters of enzyme production and cell mass formation were correlated. Enzyme synthesis was regulated by an induction mechanism and growth-dependent repression mechanism. Thermodynamic analysis revealed that the cell system exerted protection against thermal inactivation. A partially purified enzyme showed good stability when incubated at 60 degrees C and was quite stable at a pH of 5.0-7.0 and may be exploited for commercial applications.
Rajoka
M I
MI
National Institute for Biotechnology and Genetic Engineering, P.O. Box 577, Faisalabad, Pakistan. mirajoka@nibge.org <mirajoka@nibge.org>
D002244:Carbon / Q000737:chemistry / Q000378:metabolism; D004789:Enzyme Activation; D004795:Enzyme Stability; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007716:Kluyveromyces / Q000201:enzymology* / Q000378:metabolism; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D013816:Thermodynamics; D014995:Xylosidases / Q000378:metabolism*
D002244:Carbon; D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase; D009584:Nitrogen