Cellular uptake of magnetic nanoparticle is mediated through energy-dependent endocytosis in A549 cells.
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery, bioimaging, and biosensors, detailed information of the cellular uptake process of the nanoparticles is essential. Thus, this study was performed to elucidate the precise mechanism by which the lung cancer cells uptake the magnetic nanoparticles. Lung cells were chosen for this study because inhalation is the most likely route of exposure and lung cancer cells were also found to uptake magnetic nanoparticles rapidly in preliminary experiments. The lung cells were pretreated with different metabolic inhibitors. Our results revealed that low temperature disturbed the uptake of magnetic nanoparticles into the cells. Metabolic inhibitors also prevented the delivery of the materials into cells. Use of TEM clearly demonstrated that uptake of the nanoparticles was mediated through endosomes. Taken together, our results demonstrate that magnetic nanoparticles can be internalized into the cells through an energy-dependent endosomal-lysosomal mechanism.
Kim
Jun-Sung
JS
Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Yoon
Tae-Jong
TJ
Yu
Kyeong-Nam
KN
Noh
Mi-Suk
MS
Woo
Minah
M
Kim
Byung-Geol
BG
Lee
Kee-Ho
KH
Sohn
Byung-Hyuk
BH
Park
Seung-Bum
SB
Lee
Jin-Kyu
JK
Cho
Myung-Haing
MH
D001672:Biocompatible Materials / Q000493:pharmacokinetics*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000502:physiology; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D018942:Macrolides / Q000494:pharmacology; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000008:administration & dosage*; D019810:Sodium Azide / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature
D001672:Biocompatible Materials; D018942:Macrolides; C057620:bafilomycin A; D013395:Sucrose; D019810:Sodium Azide