[A preliminary study in establishment of mice model of experimental uveal melanoma].
OBJECTIVE
To explore the methods to establish a nude mice model of experimental uveal melanoma by the implantation the primary cultured cells into anterior chamber or subcutaneous injection.


METHODS
35 nude mice, which applied with the SPF grade standard by national healthy department, were divided into 3 groups: anterior chamber group (15), subcutaneous A group (10) and subcutaneous B group (10), respectively. (2 - 3) x 10(5) melanoma cells were inoculated into the anterior chamber of each nude mouse, while 2 x 10(6) cells were inoculated into every subcutaneous of the hind legs in A group. Besides this, the fresh mass of the tumor come from enucleated eye were transplanted into every subcutaneous of the hind legs in B group. Then the condition of transplanted tumor were observed under the slit lamp and naked eye, all nude mice were followed for 3 months the rates of tumor induction were compared.


RESULTS
The results of the rates of tumor induction in the 3 groups were 7 (46.6%) of 15, 2 (20%) of 10 and 0 of 10, respectively. The discrepancy were of significance (chi(2) = 7.080, P = 0.029).


CONCLUSIONS
Uveal melanoma can be induced successfully by inoculation the primary cultured human uveal melanoma cells into anterior chamber or subcutaneous in nude mice. The results suggest that the ration of the tumor growth is higher in subcutaneous mass transplant than any other groups.
Cheng
Hao
H
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. chrischenghao@sina.com
Wu
Zhong-yao
ZY
Zheng
Jian-liang
JL
Yang
Hua-sheng
HS
Yan
Jian-hua
JH
Huang
Dan-ping
DP
Xia
Yang
Y
D000328:Adult; D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D014604:Uveal Neoplasms / Q000473:pathology*