Structure-based cross-linking of NF-kappaB p50 homodimer and decoy bearing a novel 2'-disulfide trapping site.
Double-stranded oligodeoxyribonucleotides with engineered disulfide units were successfully used for covalent trapping of cysteine containing proteins. In particular, an efficient cross-linking of NF-kappaB p50 homodimer to a sequence-specific decoy was demonstrated. The results suggest that the synthetic oligonucleotides bearing a novel 2'-disulfide trapping site can be used as new tools to study and manipulate biological systems.
Metelev
Valeri
V
Chemistry Department, M.V. Lomonossov Moscow State University, Leninskie Gori, Moscow, Russia.
Romanenkov
Andrey
A
Kubareva
Elena
E
Zubin
Evgeniy
E
Polouchine
Nikolai
N
Zatsepin
Timofei
T
Molochkov
Nikolai
N
Oretskaya
Tatiana
T
D003432:Cross-Linking Reagents / Q000737:chemistry*; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry*; D052002:NF-kappa B p50 Subunit / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry
D003432:Cross-Linking Reagents; D004220:Disulfides; D052002:NF-kappa B p50 Subunit; D009838:Oligodeoxyribonucleotides