title: Short regions of sequence identity between the genomes of human and rodent parvoviruses and their respective hosts occur within host genes for the cytoskeleton, cell adhesion and Wnt signalling.
abstract: Our understanding of the mechanism(s) of pathogenesis and persistence of vertebrate parvoviruses remains incomplete. With the recent availability of the complete genome sequences of human, rat and mouse, and the ability to search these sequences and to locate matches to exact genomic regions, further insight into the interaction of parvoviruses with their human and rodent hosts is possible. To determine the extent and nature of sequence identity between candidate parvoviruses and their respective hosts, blast searches of the genome sequences of adeno-associated virus, parvovirus B19, mouse parvovirus, the prototype strain and immunosuppressant variant of minute virus of mouse, Kilham rat virus and rat parvovirus were performed against the genome(s) of their respective hosts (human, rat and mouse) using the resources of the NCBI and the Celera Discovery System. Regions of identity and similarity were mapped to their precise location in their particular host genome. For each virus, between one and 12 identical regions were found. Each identical region was 17-26 nt and was generally found at multiple sites within the particular host genome. These identical regions were predominantly located in non-coding regions of particular host genes and in intergenic regions. The ontology of host genes in which identical regions were found for each of the nine virus-host interactions highlighted several pathways/processes, including the cytoskeleton, cell adhesion and Wnt signalling. Within each virus species, these homologous regions were highly conserved (100 % identity in 16 out of 23 alignments where more than one sequence was available). All of these aspects suggest a particular advantage to the viruses of the presence of these sequences.
authors.affiliation: Department of Cellular and Molecular Medicine, St George's University of London, Cranmer Terrace, London SW17 0RE, UK.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Kerr
authors.affiliation: ZLB Behring AG, Wankdorfstrasse 10, CH-3000 Bern 22, Switzerland.
authors.forename: Nicola
authors.initials: N
authors.lastname: Boschetti
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000235:genetics; D019295:Computational Biology; D003599:Cytoskeleton / Q000235:genetics; D021901:DNA, Intergenic; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D051379:Mice; D017992:Parvovirus / Q000235:genetics*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction / Q000235:genetics; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: D021901:DNA, Intergenic; D004279:DNA, Viral; D051153:Wnt Proteins