Modulation of ATP-induced inward currents by docosahexaenoic acid and other fatty acids in rat nodose ganglion neurons.
The effects of docosahexaenoic acid (DHA) and other fatty acids on P2X-receptor-mediated inward currents in rat nodose ganglion neurons were studied using the nystatin perforated patch-clamp technique. DHA accelerated the desensitization rate of the ATP-induced current. DHA showed use-dependent inhibition of the peak ATP-induced current. Other polyunsaturated fatty acids, such as arachidonic acid and eicosapentaenoic acid, displayed a similar use-dependent inhibition. The inhibitory effects of saturated fatty acids including palmitic acid and arachidic acid were weaker than those of polyunsaturated fatty acids. The results suggest that fatty acids may modulate the P2X receptor-mediated response when the channel is in the open-state.
Eto
Kei
K
Department of Physiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Arimura
Yukiko
Y
Mizuguchi
Hiroko
H
Nishikawa
Masazumi
M
Noda
Mami
M
Ishibashi
Hitoshi
H
D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D000818:Animals; D003627:Data Interpretation, Statistical; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D005227:Fatty Acids / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009620:Nodose Ganglion / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000502:physiology
D005227:Fatty Acids; D007473:Ion Channels; D018048:Receptors, Purinergic P2; D004281:Docosahexaenoic Acids; D000255:Adenosine Triphosphate