Beta-adaptin: key molecule for microglial scavenger receptor function under oxidative stress.
Scavenger receptors internalize chemically modified low density lipoprotein particles (ac-LDL) and other ligands through the process of receptor-mediated endocytosis. During this investigation using amyloid-beta as a natural ligand for the SR, we studied under a ligand-induced oxidative stress condition, changes in protein expression of several adaptor proteins important in the organization of the endocytic machinery in microglia and macrophages. Differential expression experiments of beta-adaptin, alpha-adaptin, SR-AI, and SR-BI in RAW (macrophages) and EOC (microglia) cells were performed according to dosage and exposure time to amyloid-beta. Our results show that according to dosage, amyloid-beta produces an oxidative stress state that importantly affects the availability of beta-adaptin. Under these conditions, RT-PCR assays show that beta-adaptin mRNA is normally synthesized, reason why protein translation or protein structure of beta-adaptin might be altered. These observations might have impact in the understanding of the mechanisms microglia employ to process amyloid-beta in the brain.
Manzano-León
Natalia
N
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México, DF, Mexico.
Delgado-Coello
Blanca
B
Guaderrama-Díaz
Margarita
M
Mas-Oliva
Jaime
J
D033966:Adaptor Protein Complex beta Subunits / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000008:administration & dosage*; D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051116:Receptors, Scavenger / Q000378:metabolism*
D033966:Adaptor Protein Complex beta Subunits; D016229:Amyloid beta-Peptides; D051116:Receptors, Scavenger