title: Isolation of chloroplast proteins from Arabidopsis thaliana for proteome analysis.
abstract: This chapter describes a simple protocol for large-scale chloroplast purification for proteome analysis. The protocol has not been tested for protein import activity but is optimized for chloroplast proteome yield and purity and minimal protein degradation.
authors.affiliation: Department of Plant Biology, Cornell University, Ithaca, New York, USA.
authors.forename: Klaas J
authors.initials: KJ
authors.lastname: van Wijk
authors.forename: Jean-Benoit
authors.initials: JB
authors.lastname: Peltier
authors.forename: Lisa
authors.initials: L
authors.lastname: Giacomelli
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D002458:Cell Fractionation; D002736:Chloroplasts / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D012996:Solutions
chemical_list: D010940:Plant Proteins; D012996:Solutions