[DNA amplification of Plasmodium vivax parasites from Giemsa-stained blood smears].
OBJECTIVE
To develop methods of extracting DNA from malaria parasites on Giemsa-stained blood smears.


METHODS
Improved Na2HPO4 method and Chelex-100 ion-exchange technique were used to extract DNA from Giemsa-stained or unstained blood smears. Nested PCR was employed for amplification and identification of allelotype in the Plasmodium vivax merozoite surface protein-1 (PvMSP-1).


RESULTS
Target DNA bands appeared in all samples of unstained thick blood smears, while no DNA bands were visible in the fixed and stained thin smears. Both methods identified PvMSP-1 alleles from smears with parasitemia of > or = 0.01%.


CONCLUSIONS
It is feasible to identify PvMSP-1 alleles from Giemsa-stained blood smear.
Xiao
Fang-zhen
FZ
Fujian Provincial Center for Diseases Control and Prevention, Fuzhou, China.
Zhang
Shan-ying
SY
Xu
Long-shan
LS
Huang
Jiang-hong
JH
Xie
Han-guo
HG
Ou
Yang-rong
YR
D000483:Alleles; D000818:Animals; D001399:Azure Stains; D016054:DNA, Protozoan / Q000097:blood*; D006801:Humans; D010966:Plasmodium vivax / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
D001399:Azure Stains; D016054:DNA, Protozoan