title: Expression of the iron-activated nspA and secY genes in Neisseria meningitidis group B by Fur-dependent and -independent mechanisms.
abstract: Our whole-genome microarray studies of Neisseria meningitidis MC58 previously identified a set of 153 genes whose transcription was activated during growth in iron. In this study, Fur-mediated regulation of the iron-activated nspA gene was confirmed, whereas iron-activated regulation of the secY gene was demonstrated to be Fur independent. Analysis of the Fur binding sequences in the nspA gene and an additional iron-activated and Fur-regulated gene identified a hexameric (G/T)ATAAT unit in the operator regions of these genes similar to that observed in Fur- and iron-repressed genes. These studies indicate that the expression of the iron-activated nspA and secY genes in N. meningitidis occur by Fur-dependent and -independent mechanisms, respectively.
authors.affiliation: Department of Medicine, Section of Molecular Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
authors.forename: Yazdani B
authors.initials: YB
authors.lastname: Shaik
authors.forename: Susan
authors.initials: S
authors.lastname: Grogan
authors.forename: Michael
authors.initials: M
authors.lastname: Davey
authors.forename: Shite
authors.initials: S
authors.lastname: Sebastian
authors.forename: Sulip
authors.initials: S
authors.lastname: Goswami
authors.forename: Borys
authors.initials: B
authors.lastname: Szmigielski
authors.forename: Caroline A
authors.initials: CA
authors.lastname: Genco
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D018983:DNA Footprinting; D024202:Electrophoretic Mobility Shift Assay; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D007501:Iron / Q000378:metabolism; D038541:Neisseria meningitidis, Serogroup B / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C105506:NspA protein, Neisseria; D009841:Oligonucleotides; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; D007501:Iron