[Kinetic properties of butyrylcholinesterases immobilised on pH-sensitive field-effect transistor surface and inhibitory action of steroidal glycoalkaloids on these enzymes].
The inhibitory action of steroid glycoalkaloids alpha-solanine, alpha-chaconine and tomatine on horse and human serum butyryl cholinesterases immobilized on the pH-sensitive field-effect transistors has been studied. Using acetyl- and butyryl choline as substrates, the optimal pH and the apparent kinetic parameters (< K(m) >, < V(max) >) of immobilized butyryl cholinesterases have been calculated in the absence of inhibitors. The affinity of each enzyme to glycoalkaloids has been estimated from calculation of apparent inhibition constants < K(i) > and inhibition coefficients i(0.5). Application of the studied cholinesterases for biosensoric determination of glycoalkaloids in the wide range of concentrations (10(-7)-10(-4) M) in different media has been discussed.
Benilova
I V
IV
Arkhypova
V M
VM
Dziadevych
S V
SV
Jaffrezic-Renault
N
N
Martelet
C
C
Soldatkin
O P
OP
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002091:Butyrylcholinesterase / Q000737:chemistry*; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012680:Sensitivity and Specificity; D012991:Solanaceous Alkaloids / Q000032:analysis* / Q000737:chemistry; D014173:Transistors, Electronic
D002800:Cholinesterase Inhibitors; D004800:Enzymes, Immobilized; D012991:Solanaceous Alkaloids; D002091:Butyrylcholinesterase