title: New crystal forms of Diocleinae lectins in the presence of different dimannosides.
abstract: Studying the interactions between lectins and sugars is important in order to explain the differences observed in the biological activities presented by the highly similar proteins of the Diocleinae subtribe. Here, the crystallization and preliminary X-ray data of Canavalia gladiata lectin (CGL) and C. maritima lectin (CML) complexed with Man(alpha1-2)Man(alpha1)OMe, Man(alpha1-3)Man(alpha1)OMe and Man(alpha1-4)Man(alpha1)OMe in two crystal forms [the complexes with Man(alpha1-3)Man(alpha1)OMe and Man(alpha1-4)Man(alpha1)OMe crystallized in space group P3(2) and those with Man(alpha1-2)Man(alpha1)OMe crystallized in space group I222], which differed from those of the native proteins (P2(1)2(1)2 for CML and C222 for CGL), are reported. The crystal complexes of ConA-like lectins with Man(alpha1-4)Man(alpha1)OMe are reported here for the first time.
authors.affiliation: Departamento de Física-IBILCE-Universidade Estadual Paulista, São José do Rio Preto 15054-000, Brazil.
authors.forename: Frederico Bruno Mendes Batista
authors.initials: FB
authors.lastname: Moreno
authors.forename: Gustavo Arruda
authors.initials: GA
authors.lastname: Bezerra
authors.forename: Taianá Maia
authors.initials: TM
authors.lastname: de Oliveira
authors.forename: Emmanuel Prata
authors.initials: EP
authors.lastname: de Souza
authors.forename: Bruno Anderson Matias
authors.initials: BA
authors.lastname: da Rocha
authors.forename: Raquel Guimarães
authors.initials: RG
authors.lastname: Benevides
authors.forename: Plínio
authors.initials: P
authors.lastname: Delatorre
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
authors.forename: Walter Filgueira
authors.initials: WF
authors.lastname: de Azevedo
mesh_terms: D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry; D018360:Crystallography, X-Ray; D007887:Fabaceae / Q000737:chemistry*; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D037121:Plant Lectins