Down-regulation of RUNX1, RUNX3 and CBFbeta in hepatocellular carcinomas in an early stage of hepatocarcinogenesis.
BACKGROUND
Our previous studies suggested that deficient function of RUNX3 protein is causally related to development and progression of human gastric cancer. RUNX3 is mapped to 1p36, which is frequently deleted in hepatocellular carcinomas (HCC), therefore, these tumors were investigated for expression and copy number changes of RUNX3 and other Runt-related genes, RUNX1, RUNX2, and their co-factor CBFP. Similarly nearby uninvolved liver showing cirrhosis or normal histology was investigated in conjunction with various clinicopathological factors.


METHODS
Copy number change and expression change of RUNX family genes in 35 hepatocellular carcinoma specimens and adjoining liver with cirrhosis (LC) or normal histology were estimated using quantitative reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization.
Miyagawa
Kouji
K
Department of Surgery and Regenerative Medicine, Division of Surgery and Physiology of Digestive System, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kawaramachi-dori, Kyoto 602-8566, Japan.
Sakakura
Chouhei
C
Nakashima
Susumu
S
Yoshikawa
Tetsuji
T
Kin
Shuichi
S
Nakase
Yuenn
Y
Ito
Kosei
K
Yamagishi
Hisakazu
H
Ida
Hiroshi
H
Yazumi
Shujiro
S
Chiba
Tsutomu
T
Ito
Yoshiaki
Y
Hagiwara
Akeo
A
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics*; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics*; D050657:Core Binding Factor alpha Subunits / Q000235:genetics*; D017931:DNA Primers; D015536:Down-Regulation; D017353:Gene Deletion; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008099:Liver / Q000378:metabolism; D008103:Liver Cirrhosis / Q000235:genetics; D008113:Liver Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D050676:Core Binding Factor Alpha 2 Subunit; D050677:Core Binding Factor Alpha 3 Subunit; D050657:Core Binding Factor alpha Subunits; D017931:DNA Primers; C493728:RUNX1 protein, human; C493729:Runx3 protein, human; C406688:core binding factor alpha