Role of salicylic acid in alleviating oxidative damage in rice roots (Oryza sativa) subjected to cadmium stress.
Time-dependent changes in enzymatic and non-enzymatic antioxidants, and lipid peroxidation were investigated in roots of rice (Oryza sativa) grown hydroponically with Cd, with or without pretreatment of salicylic acid (SA). Exposure to 50 microM Cd significantly decreased root growth, and activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), but increased the concentrations of H(2)O(2), malondialdehyde (MDA), ascorbic acid (AsA), glutathione (GSH) and non-protein thiols (NPT). However, pretreatment with 10 microM SA enhanced the activities of antioxidant enzymes and the concentrations of non-enzymatic antioxidants, but lowered the concentrations of H(2)O(2) and MDA in the Cd-stressed rice compared with the Cd treatment alone. Pretreatment with SA alleviated the Cd-induced inhibition of root growth. The results showed that pretreatment with SA enhanced the antioxidant defense activities in Cd-stressed rice, thus alleviating Cd-induced oxidative damage and enhancing Cd tolerance. The possible mechanism of SA-induced H(2)O(2) signaling in mediating Cd tolerance was discussed.
Guo
B
B
Department of Plant Nutrition, College of Natural Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, PR China.
Liang
Y C
YC
Zhu
Y G
YG
Zhao
F J
FJ
D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000032:analysis; D002104:Cadmium / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D004781:Environmental Exposure / Q000009:adverse effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005978:Glutathione / Q000032:analysis; D006861:Hydrogen Peroxide / Q000032:analysis; D018527:Hydroponics; D008315:Malondialdehyde / Q000032:analysis; D012275:Oryza / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry* / Q000187:drug effects; D020156:Salicylic Acid / Q000032:analysis / Q000494:pharmacology*; D012989:Soil Pollutants / Q000633:toxicity*; D013438:Sulfhydryl Compounds / Q000032:analysis; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D004791:Enzyme Inhibitors; D012989:Soil Pollutants; D013438:Sulfhydryl Compounds; D002104:Cadmium; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D020156:Salicylic Acid; D001205:Ascorbic Acid