[Combined expression of TSO45W-4BX from Taenia solium and porcine CD58 in Escherichia coli].
OBJECTIVE
To express the TSO45W-4BX of Taenia solium in combination with CD58 as a molecular adjuvant for improving the protective efficacy of the TSO45W-4BX recombinant vaccine.


METHODS
TSO45W-4BX and porcine CD58 genes were amplified by PCR, using recombinant plasmids pGEM-4B and pGEM-CD58 as template respectively. The CD58 fragment was inserted into the recombinant plasmid pGEX-4T-1 with directly ligated TSO45W-4BX. The transformant was induced with IPTG and followed by identifying the integrity of the recombinant containing TS045W-4BX and porcine CD58 with PCR and sequencing. The products were analyzed by SDS-PAGE and Western blotting.


RESULTS
The expression products of Mr 69,000 GST-4BX/CD58 and Mr 41,000 GST-4BX were present mainly in the form of inclusion bodies and soluble substance respectively, and both were recognized by sera of cysticercosis patients.


CONCLUSIONS
The TSO45W-4BX co-expressed with porcine CD58 conserves its immune reactivity.
Luo
Xue-nong
XN
Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, CAAS, Lanzhou, China.
Zheng
Ya-dong
YD
Dou
Yong-xi
YX
Hou
Jun-lin
JL
Jing
Zhi-zhong
ZZ
Cai
Xue-peng
XP
D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis* / Q000235:genetics; D018968:CD58 Antigens / Q000096:biosynthesis*; D003551:Cysticercosis / Q000097:blood* / Q000469:parasitology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010957:Plasmids; D016133:Polymerase Chain Reaction; D013552:Swine; D041201:Taenia solium / Q000235:genetics / Q000276:immunology*
D000953:Antigens, Protozoan; D018968:CD58 Antigens