title: Down-regulation of RUNX1, RUNX3 and CBFbeta in hepatocellular carcinomas in an early stage of hepatocarcinogenesis.
abstract: BACKGROUND
  Our previous studies suggested that deficient function of RUNX3 protein is causally related to development and progression of human gastric cancer. RUNX3 is mapped to 1p36, which is frequently deleted in hepatocellular carcinomas (HCC), therefore, these tumors were investigated for expression and copy number changes of RUNX3 and other Runt-related genes, RUNX1, RUNX2, and their co-factor CBFP. Similarly nearby uninvolved liver showing cirrhosis or normal histology was investigated in conjunction with various clinicopathological factors.
  
  
  METHODS
  Copy number change and expression change of RUNX family genes in 35 hepatocellular carcinoma specimens and adjoining liver with cirrhosis (LC) or normal histology were estimated using quantitative reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization.
authors.affiliation: Department of Surgery and Regenerative Medicine, Division of Surgery and Physiology of Digestive System, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kawaramachi-dori, Kyoto 602-8566, Japan.
authors.forename: Kouji
authors.initials: K
authors.lastname: Miyagawa
authors.forename: Chouhei
authors.initials: C
authors.lastname: Sakakura
authors.forename: Susumu
authors.initials: S
authors.lastname: Nakashima
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kin
authors.forename: Yuenn
authors.initials: Y
authors.lastname: Nakase
authors.forename: Kosei
authors.initials: K
authors.lastname: Ito
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yamagishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ida
authors.forename: Shujiro
authors.initials: S
authors.lastname: Yazumi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Chiba
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Ito
authors.forename: Akeo
authors.initials: A
authors.lastname: Hagiwara
mesh_terms: D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics*; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics*; D050657:Core Binding Factor alpha Subunits / Q000235:genetics*; D017931:DNA Primers; D015536:Down-Regulation; D017353:Gene Deletion; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008099:Liver / Q000378:metabolism; D008103:Liver Cirrhosis / Q000235:genetics; D008113:Liver Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D050677:Core Binding Factor Alpha 3 Subunit; D050657:Core Binding Factor alpha Subunits; D017931:DNA Primers; C493728:RUNX1 protein, human; C493729:Runx3 protein, human; C406688:core binding factor alpha