title: First observation of cylindrospermopsin in Anabaena lapponica isolated from the boreal environment (Finland).
abstract: The cyanobacterial cytotoxin cylindrospermopsin has been mostly associated with cyanobacteria present in tropical and subtropical regions. Cylindrospermopsin has recently been found in cyanobacterial samples in central and southern Europe but the possible presence of the toxin in northern Europe has been unknown. Fifty-eight field and laboratory culture samples of Finnish cyanobacteria were analyzed by high-performance liquid chromatography combined with UV diode-array detection, multiple reactant monitoring in a triple-quadrupole mass spectrometer (MS), and accurate mass measurements using a time-of-flight MS instrument. Cylindrospermopsin was confirmed by all three techniques in a culture sample of Anabaena lapponica at a concentration of 242 microg cylindrospermopsin per g freeze-dried cyanobacterial material.
authors.affiliation: Department of Biochemistry and Pharmacy, Abo Akademi University, Tykistökatu 6A, FI-20520 Turku, Finland.
authors.forename: Lisa
authors.initials: L
authors.lastname: Spoof
authors.forename: Katri A
authors.initials: KA
authors.lastname: Berg
authors.forename: Jarkko
authors.initials: J
authors.lastname: Rapala
authors.forename: Kirsti
authors.initials: K
authors.lastname: Lahti
authors.forename: Liisa
authors.initials: L
authors.lastname: Lepistö
authors.forename: James S
authors.initials: JS
authors.lastname: Metcalf
authors.forename: Geoffrey A
authors.initials: GA
authors.lastname: Codd
authors.forename: Jussi
authors.initials: J
authors.lastname: Meriluoto
mesh_terms: D000470:Alkaloids; D017033:Anabaena / Q000737:chemistry / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D001427:Bacterial Toxins; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D000087522:Cyanobacteria Toxins; D005387:Finland; D005612:Freeze Drying / Q000379:methods; D005618:Fresh Water / Q000382:microbiology*; D013058:Mass Spectrometry / Q000379:methods; D012623:Seawater / Q000382:microbiology*; D014498:Uracil / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism
chemical_list: D000470:Alkaloids; D001427:Bacterial Toxins; D003470:Culture Media; D000087522:Cyanobacteria Toxins; C089595:cylindrospermopsin; D014498:Uracil