Immunolocalization of duodenal cytochrome B: a relationship with circulating markers of iron status.
BACKGROUND
The brush border ferric reductase (Dcytb) is critical for the absorption of dietary iron and appears to be expressed on the duodenal enterocyte brush border. The Dcytb expression is increased in severe iron-deficient anaemia, but the situation in a more typical mild iron deficiency is unclear. This study investigated Dcytb expression in patients with normal iron status or mild iron deficiency and its relationships with enterocyte iron status.


METHODS
Duodenal biopsy specimens and blood samples were obtained from 32 patients undergoing routine upper gastrointestinal endoscopy. Twenty-three specimens (six iron-deficient and 17 iron-replete) were processed for light-microscopy (LM) and for immunohistochemistry with antibodies against Dcytb and heavy/light chain ferritin subunits. The nine remaining biopsies (three iron-deficient and six iron-replete) were processed for electron microscopy (EM). Immunolocalization of Dcytb and intracellular ferritin was performed with appropriate primary antibodies followed by 10-nm gold conjugate labels.


RESULTS
The LM process showed a strong negative correlation between immunolabelling intensity of Dcytb on the enterocyte brush border and serum iron saturation (P < 0.001), but only a weak negative correlation between this antigen and haemoglobin (P = 0.08) or serum ferritin concentrations (P = 0.4). EM confirmed anti-Dcytb preferential labelling of microvilli rather than enterocyte cytoplasm (P = 0.001), but preferential antiferritin labelling of cytoplasm (P < 0.02). There was no correlation with enterocyte cytoplasmic ferritin labelling (i.e. enterocyte iron status and Dcytb expression).


CONCLUSIONS
Enterocyte Dcytb brush border expression is increased even in mild iron deficiency and may be related to serum iron saturation. The lack of correlation with enterocyte ferritin expression deserves further study with direct measurement of intracellular iron.
Li
A C Y
AC
Gastrointestinal Laboratory, St Thomas' Hospital, London, UK. drandy@btinternet.com
Warley
A
A
Thoree
V
V
Simpson
R J
RJ
McKie
A T
AT
Kodjabashia
K
K
Thompson
R P H
RP
Powell
J J
JJ
D015415:Biomarkers / Q000097:blood; D003573:Cytochrome b Group / Q000378:metabolism*; D004386:Duodenum / Q000378:metabolism*; D005293:Ferritins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007408:Intestinal Absorption / Q000502:physiology; D007413:Intestinal Mucosa / Q000648:ultrastructure; D007501:Iron / Q000378:metabolism*; D008854:Microscopy, Electron
D015415:Biomarkers; D003573:Cytochrome b Group; D005293:Ferritins; D007501:Iron