title: Kinin B1 and B2 receptors are overexpressed in the hippocampus of humans with temporal lobe epilepsy.
abstract: Molecular biology tools have been employed to investigate the participation of peptides in human temporal lobe epilepsy (TLE). Active polypeptides and their receptors have been related to several brain processes, such as inflammation, apoptosis, brain development, K(+) and Ca(2+) channels' activation, cellular growth, and induction of neuronal differentiation. Previous works have shown a neuroprotector effect for kinin B2 receptor and a deleterious, pro-epileptogenic action for kinin B1 receptor in animal models of TLE. The present work was delineated to analyze the kinin B1 and B2 receptors expression in the hippocampus of patients presenting refractory mesial TLE. The hippocampi were removed during the patients surgery in a procedure used for seizure control and compared with tissues obtained after autopsy. Nissl staining was performed to study the tissue morphology and immunohistochemistry, and Western blot was used to compare the distribution and levels of both receptors in the hippocampus. In addition, real time PCR was employed to analyze the gene expression of these receptors. Nissl staining showed sclerotic hippocampi with hilar, granular, and pyramidal cell loss in TLE patients. Immunohistochemistry and Western blot analyses showed increased expression of kinin B1 and B2 receptors but the real-time PCR data demonstrated increased mRNA level only for kinin B2 receptors, when compared with controls. These data show for the first time a relationship between human TLE and the kallikrein-kinin system, confirming ours previous results, obtained from experimental models of epilepsy.
authors.affiliation: Departamento de Neurologia Experimental, Universidade Federal de São Paulo, Sao Paulo, Brazil.
authors.forename: Sandra Regina
authors.initials: SR
authors.lastname: Perosa
authors.forename: Gustavo Adolfo
authors.initials: GA
authors.lastname: Argañaraz
authors.forename: Eduardo Massatoshi
authors.initials: EM
authors.lastname: Goto
authors.forename: Luciana Gilbert Pessoa
authors.initials: LG
authors.lastname: Costa
authors.forename: Ana Carla
authors.initials: AC
authors.lastname: Konno
authors.forename: Pedro Paulo Vasconcellos
authors.initials: PP
authors.lastname: Varella
authors.forename: Joselita Ferreira Carvalho
authors.initials: JF
authors.lastname: Santiago
authors.forename: João Bosco
authors.initials: JB
authors.lastname: Pesquero
authors.forename: Mauro
authors.initials: M
authors.lastname: Canzian
authors.forename: Debora
authors.initials: D
authors.lastname: Amado
authors.forename: Elza Marcia
authors.initials: EM
authors.lastname: Yacubian
authors.forename: Henrique
authors.initials: H
authors.lastname: Carrete
authors.forename: Ricardo Silva
authors.initials: RS
authors.lastname: Centeno
authors.forename: Esper Abrão
authors.initials: EA
authors.lastname: Cavalheiro
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Silva
authors.forename: Maria da Graça Naffah
authors.initials: Mda G
authors.lastname: Mazzacoratti
mesh_terms: D000328:Adult; D015153:Blotting, Western / Q000379:methods; D004833:Epilepsy, Temporal Lobe / Q000473:pathology*; D015870:Gene Expression / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis; D043783:Receptor, Bradykinin B1 / Q000235:genetics / Q000378:metabolism*; D043782:Receptor, Bradykinin B2 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D012333:RNA, Messenger; D043783:Receptor, Bradykinin B1; D043782:Receptor, Bradykinin B2