title: [Association between calcium-sensing receptor protein expression and rat cardiomyocyte apoptosis].
abstract: OBJECTIVE
  To investigate the relationship between calcium-sensing receptor protein (CaSR) expression and rat cardiomyocyte apoptosis and related signal transduction pathways.
  
  
  METHODS
  The CaSR, BCl2, Caspase3 protein and ERK1/2 phosphorylation or non-phosphorylation were detected by Western blot. Cardiomyocyte apoptosis was detected by flow cytometry and immunofluorescence.
  
  
  RESULTS
  CaSR protein was detected in rat cardiac tissue and CaSR activator gadolinium (GdCl3) induced cardiomyocyte apoptosis and increased ERK1/2 phosphorylation and expression of BCl2 and activated Caspase3. The selective mitogen-activated protein kinase (MAPK) inhibitor PD98059 abolished gadolinium -induced ERK1/2 activation and BCl2 expression, further increased the activation of Caspase3 and cardiomyocyte apoptosis.
  
  
  CONCLUSIONS
  Our results demonstrate the CaSR existence in cardiomyocytes and CaSR activation by gadolinium can induce myocyte apoptosis by activating Caspase3 and tyrosine protein kinase pathway.
authors.affiliation: Department of Pathophysiology, Harbin Medical University, Harbin 150086, China.
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Sun
authors.forename: Chang-qing
authors.initials: CQ
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Sa
authors.initials: S
authors.lastname: Shi
authors.forename: Wei-hua
authors.initials: WH
authors.lastname: Zhang
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Zhao
authors.forename: Yan-qiao
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Han
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Han
authors.forename: Chun-ming
authors.initials: CM
authors.lastname: Jiang
authors.forename: Quan-feng
authors.initials: QF
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D005260:Female; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D044169:Receptors, Calcium-Sensing / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction
chemical_list: D012333:RNA, Messenger; D044169:Receptors, Calcium-Sensing; D053148:Caspase 3