Short monolithic columns--a breakthrough in purification and fast quantification of tomato mosaic virus.
Drawbacks of conventional virus purification methods have led to the development of new, mostly chromatography-based methods. Short monolithic columns are stationary phases intended for purification of large molecules. In this work efficient chromatographic purification of tomato mosaic virus (ToMV) from plant material is described. Based on short monolithic column, the purification process was shortened from 5 days to 2 hours. High viral purity was achieved and recovery of chromatographic step was up to 90%. In addition, these columns enabled preliminary quantification of the virus in just a few minutes, much faster than other quantification methods (e.g. enzyme-linked immunosorbent assay or real-time polymerase chain reaction) which take 1-2 days. These results demonstrate the potential of short monolith column technology for purification and analysis of different viruses.
Kramberger
Petra
P
BIA Separations, d.o.o., Teslova 30, SI-1000 Ljubljana, Slovenia. petra.kramberger@monoliths.com
Peterka
Matjaz
M
Boben
Jana
J
Ravnikar
Maja
M
Strancar
Ales
A
D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D015203:Reproducibility of Results; D013997:Time Factors; D014027:Tobacco Mosaic Virus / Q000302:isolation & purification*