title: Oxidative modification of cytochrome c by hydrogen peroxide.
abstract: Oxidative alteration of mitochondrial cytochrome c has been linked to disease and is one of the causes of pro-apoptotic events. We have investigated the modification of cytochrome c by H2O2. When cytochrome c was incubated with H2O2, oligomerization of the protein increased and the formation of carbonyl derivatives and dityrosine was stimulated. Radical scavengers prevented these effects suggesting that free radicals are implicated in the H2O2-mediated oligomerization. Oligomerization was significantly inhibited by the iron chelator, deferoxamine. During incubation of deoxyribose with cytochrome c and H2O2, damage to the deoxyribose occurred in parallel with the release of iron from cytochrome c. When cytochrome c that had been exposed to H2O2 was analyzed by amino acid analysis, the tyrosine, histidine and methionine residues proved to be particularly sensitive. These results suggest that H2O2-mediated cytochrome c oligomerization is due to oxidative damage resulting from free radicals generated by a combination of the peroxidase activity of cytochrome c and the Fenton reaction of free iron released from the oxidatively-damaged protein.
authors.affiliation: Department of Genetic Engineering, Cheongju University, Korea.
authors.forename: Nam Hoon
authors.initials: NH
authors.lastname: Kim
authors.forename: Moon Sik
authors.initials: MS
authors.lastname: Jeong
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Kang
mesh_terms: D000818:Animals; D002417:Cattle; D045304:Cytochromes c / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007501:Iron; D010084:Oxidation-Reduction; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: C045076:Fenton's reagent; D014443:Tyrosine; D045304:Cytochromes c; D006861:Hydrogen Peroxide; C007543:dityrosine; D007501:Iron