[Characteristics of SRAP marker in detecting polymorphism of cucumber genome].
When SRAP (Sequence-Related Amplified Polymorphism) marker was used in constructing genetic map and analyzing QTL for high temperature resistance in cucumber (Cucumis sativus L.), it exhibited certain characteristics in detecting genomic polymorphism. When each forward primer was combined with different reverse primers, the number of primer combinations that produced polymorphism ranged from five to eight. When each reverse primer was in combination with different forward primers, the number of polymorphic primer combinations ranged from two to eleven. The reverse primers SA4 or EM6 produced identical polymorphic bands when combined with all the forward primers tested. These bands might be amplified by the reverse primers. The polymorphic bands amplified from OD3ME11 co-segregated in the F2 population. The utilization of these characteristics in our research was discussed.
Qian
Wen-Cheng
WC
College of Life Sciences, Nankai University, Tianjin 300071, China. paozhifeng1@sina.com
Zhang
Gui-Hua
GH
Chen
Fei-Xue
FX
Han
Yi-Ke
YK
Chen
De-Fu
DF
Du
Sheng-Li
SL
Chen
Xi-Wen
XW
D018553:Cucumis sativus / Q000235:genetics* / Q000502:physiology; D017931:DNA Primers; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D006358:Hot Temperature; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci
D017931:DNA Primers; D005819:Genetic Markers