Regulatory effects of vasoactive intestinal peptide on the migration of mature dendritic cells.
The neuropeptide vasoactive intestinal peptide (VIP), released within lymphoid organs from nerve terminals and/or immune cells, plays a significant anti-inflammatory role. It was reported that VIP can induce regulatory dendritic cells (DCs) and promote Th2-type responses. However, the regulatory effect of VIP on the migration and expression of chemokine receptors by DC is mostly unknown. In the present study, we show that VIP exerts a differential effect on the expression of CCR1 and CCR7 by lipopolysaccharide (LPS)-treated mature DCs (mDCs) at both protein and mRNA levels. It up-regulates CCR1 expression but down-regulates CCR7 expression in LPS-stimulated mature DC, thereby differentially regulating the migration of mature DCs in response to CCL5 and CCL19. Our data indicate that VIP functions as a key endogenous anti-inflammatory agent by inhibiting migration of mDCs to draining lymph nodes, thus preventing the induction of an inflammatory immune response.
Weng
Yuesong
Y
Institute of Immunology, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, PR China.
Sun
Juyun
J
Wu
Qianqian
Q
Pan
Jianping
J
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D054415:Chemokine CCL19; D018946:Chemokine CCL5; D019742:Chemokines, CC / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000502:physiology*; D015536:Down-Regulation; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000378:metabolism; D054389:Receptors, CCR1; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; C515461:Ccl19 protein, mouse; C503460:Ccl5 protein, mouse; C515301:Ccr1 protein, mouse; C515371:Ccr7 protein, mouse; D054415:Chemokine CCL19; D018946:Chemokine CCL5; D019742:Chemokines, CC; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D054389:Receptors, CCR1; D054400:Receptors, CCR7; D019707:Receptors, Chemokine; D014660:Vasoactive Intestinal Peptide