[A simulated study on mapping QTL in a segregating sub-population].
For mapping QTLs, phenotypes of the traits in segregating population derived from the cross between two isogenic lines of the targeted QTL may reflect its genotype if the effect of the QTL is relatively large. In order to map the QTL, it is necessary to use a large sample under the high density of markers around the QTL. However, it increases experimental costs. In order to save the costs, it is possible to map the QTL using the sub-population that consists of plants with homogenous recessive. In this paper, the sub-population was used to estimate the recombination fraction between the marker and the QTL, and its standard error for F2, backcross (BC), double haploid (DH) and recombinant inbred lines (RIL) populations, respectively. The results from Monte Carlo simulation showed that the estimation of recombination fraction based on the sub-population is consistent with that obtained from the full population, and the precision of the former is same as that of the later under the same sample size.
Lin
Fei
F
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. 0576linfei@163.com
Wan
Su-Qin
SQ
Cheng
Li-Guo
LG
Lü
Hai-Yan
HY
Li
Guang-Jun
GJ
Zhang
Yuan-Ming
YM
D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D008957:Models, Genetic; D009010:Monte Carlo Method; D040641:Quantitative Trait Loci; D011995:Recombination, Genetic; D018401:Sample Size; D012680:Sensitivity and Specificity
D005819:Genetic Markers