Oral immunization of BALB/c mice by intragastric delivery of Streptococcus gordonii-expressing Giardia cyst wall protein 2 decreases cyst shedding in challenged mice.
Giardia lamblia (Giardia duodenalis or Giardia intestinalis) is a protozoan parasite of vertebrates with broad host specificity. Specific antibodies directed against cyst antigens can interfere with the cyst wall-building process. In this study, we engineered Streptococcus gordonii to express a 26 kDa fragment of cyst wall protein 2 (CWP2), containing a relevant B cell epitope, on the cell surface. This is the first report of S. gordonii expressing a protein of parasite origin. As S. gordonii was intended for intestinal delivery of CWP2, it was determined that this oral commensal bacterium is able to persist in the murine intestine for 30 days. Immunization with recombinant streptococci expressing the 26 kDa fragment resulted in higher antibody levels. Specific anti-CWP2 IgA antibodies were detected in fecal samples and anti-CWP2 IgG antibodies were detected in serum demonstrating the efficacy of S. gordonii for intragastric antigen delivery. In a pilot challenge experiment, immunized mice demonstrated a significant 70% reduction in cyst output.
Lee
Peter
P
Institute of Parasitology, McGill University, Macdonald Campus, Ste-Anne de Bellevue, QC, Canada.
Faubert
Gaétan M
GM
D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D004195:Disease Models, Animal; D005260:Female; D016829:Giardia lamblia / Q000235:genetics / Q000276:immunology*; D005873:Giardiasis / Q000276:immunology / Q000517:prevention & control*; D007114:Immunization / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013291:Streptococcus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014612:Vaccines / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D007074:Immunoglobulin G; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D014612:Vaccines