Specific cell behavior of human fibroblast onto carbohydrate surface detected by glycoblotting films.
We synthesized an aminooxyl polymer that is reactive with the reduced end of carbohydrates using our sugar-displaying approach. The carbohydrates were easily immobilized on the polymer film (glycoblotting film) by simple immersion in a in sugar solution through stable oxime bond. The in vitro behaviors of human fibroblasts on the carbohydrate-coated surface were investigated. The adhesion of human fibroblasts on the cellobiose- and cellotriose-coated surfaces was much greater than on the other coated surfaces and the noncoated surface. This result indicated that simple structural differences in carbohydrates induced biological changes in human cells, especially cell adhesion. Our approach provides a high-throughput assay system for carbohydrate-related cell adhesion and proliferation.
Onodera
Tomohiro
T
Department of Orthopaedic Surgery, Hokkaido University School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo 060-8638, Japan.
Niikura
Kenichi
K
Iwasaki
Norimasa
N
Nagahori
Noriko
N
Shimaoka
Hideyuki
H
Kamitani
Ryusuke
R
Majima
Tokifumi
T
Minami
Akio
A
Nishimura
Shin-Ichiro
S
D002241:Carbohydrates / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans
D002241:Carbohydrates