title: Antibodies against Toxoplasma gondii in the Pacific bottlenose dolphin (Tursiops aduncus) from the Solomon Islands.
abstract: Serum samples from 58 Pacific bottlenose dolphins (Tursiops aduncus) from the Solomon Islands were tested for the IgG antibody to Toxoplasma gondii by the latex agglutination test (LAT), enzyme-linked immunosorbent assay (ELISA), and immunoblotting. The ELISA cut-off value was taken as OD > or = 0.276, and the final dilution ratio, recognized as positive, was represented by the end titer. In 25 of 58 samples, no antibody activity was detected by LAT and ELISA. In 8 of 58 samples, anti-T. gondii IgG antibodies were detected by both LAT and ELISA, with titers of greater than 1 : 64 and 1 : 160, respectively. By immunoblotting, the 8 serum samples producing higher titers showed specific antibody IgG binding to several antigens on the T. gondii lane, but not on the Neospora caninum lane. No specific bands were noted on the lanes for either parasite in the 25 serum samples for which no antibody activity was detected. The specific binding of IgG antibodies to T. gondii antigens observed for serum samples producing higher titers suggests that Pacific bottlenose dolphins from the Solomon islands are exposed to T. gondii.
authors.affiliation: Laboratory of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro 080-8555, Japan. yomata@obihiro.ac.jp
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Omata
authors.forename: Ted
authors.initials: T
authors.lastname: Hammond
authors.forename: Keiko
authors.initials: K
authors.lastname: Itoh
authors.forename: Koichi
authors.initials: K
authors.lastname: Murata
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D000918:Antibody Specificity; D050681:Bottle-Nosed Dolphin / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015151:Immunoblotting / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood; D007841:Latex Fixation Tests / Q000662:veterinary; D008542:Melanesia / Q000453:epidemiology; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000453:epidemiology* / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G