title: Carotenoids extraction from Japanese persimmon (Hachiya-kaki) peels by supercritical CO(2) with ethanol.
abstract: The extraction of carotenoids from Japanese persimmon peels by supercritical fluid extraction (SFE), of which the solvent was CO(2), was performed. In order to enhance the yield and selectivity of the extraction, some portion of ethanol (5 - 20 mol%) was added as an entrainer. The extraction temperature ranged from 313 to 353 K and the pressure was 30 MPa. The effect of temperature on the extraction yield of carotenoids was investigated at 10 mol% of the ethanol concentration in the extraction solvent, and a suitable temperature was found to be 333 K among the temperatures studied with respect to the carotenoid yield. With increasing the entrainer amount from 0 to 10 mol% at a constant temperature (333 K), the carotenoid yield in the extraction was improved, whereas the selectivity of the extracted carotenoids was drastically depressed. We also conducted qualitative and quantitative analyses for the carotenoid components in the extract by HPLC, and analyzed the extraction behavior of each individual carotenoid (alpha-carotene, beta-carotene, beta-cryptoxanthin, lycopene, lutein, and zeaxanthin). The selectivity of each carotenoid changed with the elapsed time and its time evolution was dependent on the carotenoid component, indicating that the location profile and the content can be important factors to understand the SFE behavior of each carotenoid in persimmon peels.
authors.affiliation: Research Center of Supercritical Fluid Technology, Tohoku University.
authors.forename: Mayako
authors.initials: M
authors.lastname: Takahashi
authors.forename: Hiromoto
authors.initials: H
authors.lastname: Watanabe
authors.forename: Junko
authors.initials: J
authors.lastname: Kikkawa
authors.forename: Masaki
authors.initials: M
authors.lastname: Ota
authors.forename: Masaru
authors.initials: M
authors.lastname: Watanabe
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Inomata
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Sato
mesh_terms: D002245:Carbon Dioxide; D002338:Carotenoids / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D029788:Diospyros / Q000737:chemistry*; D000431:Ethanol; D005638:Fruit / Q000737:chemistry*
chemical_list: D002245:Carbon Dioxide; D002338:Carotenoids; D000431:Ethanol