The relationship between the structure of dermatan sulfate derivatives and their antithrombotic activities.
To study the relationship between the structure of dermatan sulfate (DS) derivatives and their anti-thrombotic activities, DS-derived oligosaccharides (with different structures and relative molecular weight (M(r))) were prepared, and the effects of the DS-derived oligosaccharides on the activities of heparin cofactor II (HCII), activated protein C (APC), blood platelet, and vascular endothelial cells were studied. The major disaccharides of DS and polysulfated dermatan sulfate (PSDS) were IdoA-1-->3-GalNAc-4-OSO(3) and IdoA-2OSO(3)-1-->3-GalNAc4, 6-diOSO(3), respectively. The results showed that the consequence of the thrombotic inhibitory effects of DS and its derivatives were as follows: PSDS>low molecular weight polysulfated dermatan sulfate (LPSDS)>DS. Both DS and PSDS inhibited platelet aggregation in the concentration-dependent manner, and the IC(50) value of DS and PSDS is 12.7+/-1.3 and 28.6+/-0.9 mg/mL, respectively. DS oligosaccharides (DSOSs) and PSDS oligosaccharides (PSDSOSs) both significantly inhibited P-selectin expression on platelet surface (P<0.01), while DSOSs have no different effect compared with PSDSOSs. DSOSs and PSDSOSs significantly enhanced the activity of HCII in inhibiting thrombin in the plasma. The most active PSDSOS was PSDSOS(1) with M(r) of 4959, which enhanced the HCII activity by 91% (P<0.01). The experiments on APC activity showed that DS and its derivatives enhanced APC activity. The most active PSDSOS was PSDSOS(3) with M(r) of 2749, which enhanced the APC activity to 331+/-27% (P<0.01). DSOSs and PSDSOSs enhanced tissue plasminogen activator (t-PA) activity and reduced the plasminogen activator inhibitor (PAI) activity from cultured human umbilical vein endothelial cells (HUVEC), resulting in the ratio of t-PA/PAI going up. PSDSOSs which have the same M(r) as DSOSs produced more active effects in above assays, except for platelet aggregation.
Du
Hai-Yan
HY
Institute of Biochemical and Biotech Drugs, School of Pharmacy, Shandong University, Jinan 250012, China.
Ji
Sheng-Li
SL
Song
Hai-Feng
HF
Ye
Qi-Nong
QN
Cao
Ji-Chao
JC
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D001792:Blood Platelets / Q000378:metabolism; D002240:Carbohydrate Sequence; D002417:Cattle; D003871:Dermatan Sulfate / Q000737:chemistry / Q000494:pharmacology*; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology*; D019007:P-Selectin / Q000096:biosynthesis; D011817:Rabbits; D013329:Structure-Activity Relationship; D013552:Swine; D013917:Thrombin / Q000037:antagonists & inhibitors
D000925:Anticoagulants; D009844:Oligosaccharides; D019007:P-Selectin; D003871:Dermatan Sulfate; D013917:Thrombin