The effect of erythrodiol-3-acetate on the expressions of matrix metalloproteinase-1 and type-1 procollagen caused by ultraviolet irradiated cultured primary old aged human skin fibroblasts.
Methanol and aqueous extracts of Styrax japonica used traditionally for the treatments of skin elastic materials were screened in vitro for the matrix metalloproteinase (MMP)-1 inhibitor actions. The methylene chloride soluble fraction of methanol extract from the stems of S. japonica showed significant MMP-1 inhibition in primary old aged human skin fibroblasts caused by ultraviolet (UV) irradiation. Main triterpenoids were isolated by repeated column chromatography. Among them, the triterpenoid erythrodiol-3-acetate reduced the expression of MMP-1 and induced the expression of type-1 procollagen at the protein levels in a dose-dependent manner caused by UV irradiated cultured old aged human skin fibroblasts. Taken together, our results suggest that erythrodiol-3-acetate plays an important role in the skin aging process caused by UV irradiation.
Moon
H-I
HI
Molecular Neuroscience and Vascular Biology Laboratory, Department of Surgery, University of Kentucky Medical Center, Lexington, Kentucky 40536, USA. himun@snu.ac.kr
Lee
J
J
Chung
J H
JH
D000368:Aged; D000369:Aged, 80 and over; D002478:Cells, Cultured; D024042:Collagen Type I / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D061965:Matrix Metalloproteinase Inhibitors; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D015595:Skin Aging / Q000528:radiation effects; D032362:Styrax / Q000737:chemistry
D024042:Collagen Type I; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; C517014:erythrodiol-3-acetate; D009828:Oleanolic Acid; D020781:Matrix Metalloproteinase 1