Antioxidant activity of a novel extract from bamboo grass (AHSS) against ischemia-reperfusion injury in rat small intestine.
Production of free radical species in cells and body tissues is known to cause many pathological disorders. Therefore, free radical scavengers play an important role in the prevention of various human diseases. Bamboo grass, Sasa senanensis, is a native Japanese plant. Sasa has been used for medicine in Japan for many centuries. In this study, we investigated the antioxidative activity of Absolutely Hemicellulose Senanensis (AHSS), a novel extract from Sasa. In the first part of this study, we found that AHSS has antioxidant activities by the assay using superoxide anion-2-methyl-6-methoxyphenylethynylimidazopyrazynone (MPEC) reaction kit. We then confirmed its antioxidative activity using a rat ischemia and subsequent reperfusion (I/R) injury model. Breakdown of the intestinal wall caused by intestinal I/R was attenuated by pretreatment with AHSS. Moreover, AHSS inhibited the production of lipid peroxide by intestinal I/R. AHSS could be an important source of ingredients for use in functional foods and other applications.
Kurokawa
Toshimitsu
T
Department of Clinical Pharmaceutics & Therapeutics, Graduate School of Pharmaceutics Sciences, Hokkaido University, Sapporo, Japan.
Itagaki
Shirou
S
Yamaji
Toshihiko
T
Nakata
Chie
C
Noda
Toshihiro
T
Hirano
Takeshi
T
Iseki
Ken
K
D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007421:Intestine, Small / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology; D008054:Lipid Peroxides / Q000037:antagonists & inhibitors / Q000378:metabolism; D049449:Luminescence; D008297:Male; D008641:Mesenteric Vascular Occlusion / Q000150:complications; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D031787:Sasa / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
D000975:Antioxidants; D004791:Enzyme Inhibitors; D008054:Lipid Peroxides; D010936:Plant Extracts; D000493:Allopurinol; D014969:Xanthine Oxidase