title: Genomic analysis of a case of multifocal adenocarcinoma in ulcerative colitis.
abstract: Long-standing ulcerative colitis is associated with an elevated risk of developing colonic adenocarcinoma. A very limited group of patients present with multiple synchronous cancers. This could be due to either a multifocal presentation of the same neoplastic clone or different tumors arising in a large area of polyclonal dysplastic colonic mucosa ("field cancerization"). Here, we describe a patient with long-standing colitis and three different tumors in the rectosigmoid part of the large bowel. Clonal evaluation of the lesions was performed by array-based comparative genomic hybridization. These three neoplasms showed a comparable pattern of genomic alterations characterized by gains of chromosomes 12, 13, and 20. Noteworthy, dysplastic mucosa distal to the three cancers displayed a completely different pattern of genomic changes indicating that different cell lineages were present. In addition, all three carcinomas were microsatellite stable and revealed identical immunoprofiles for several cancer-associated genes. We conclude that these three multifocal tumors must have originated from the same preneoplastic lineage.
authors.affiliation: Department of Pathology, Erasmus Medical Center, Josephine Nefkens Institute, Dr. Molewaterplein 50, 3015GE Rotterdam, The Netherlands. h.vandekken@erasmusmc.nl
authors.forename: Herman
authors.initials: H
authors.lastname: van Dekken
authors.forename: Josiane C
authors.initials: JC
authors.lastname: Wink
authors.forename: Kees J
authors.initials: KJ
authors.lastname: Vissers
authors.forename: Ronald
authors.initials: R
authors.lastname: van Marion
authors.forename: Patrick F
authors.initials: PF
authors.lastname: Franken
authors.forename: Monique M C P
authors.initials: MM
authors.lastname: Hoogmans
authors.forename: Winand N M
authors.initials: WN
authors.lastname: Dinjens
authors.forename: W Ruud
authors.initials: WR
authors.lastname: Schouten
authors.forename: Ernst J
authors.initials: EJ
authors.lastname: Kuipers
authors.forename: C Janneke
authors.initials: CJ
authors.lastname: van der Woude
mesh_terms: D000230:Adenocarcinoma / Q000209:etiology / Q000235:genetics*; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D003093:Colitis, Ulcerative / Q000150:complications*; D003110:Colonic Neoplasms / Q000209:etiology / Q000235:genetics*; D005260:Female; D006801:Humans; D053842:Microsatellite Instability; D009693:Nucleic Acid Hybridization