Spectroelectrochemistry of type II cytochrome c3 on a glycosylated self-assembled monolayer.
A modified silver electrode was prepared by the self-assembly of a thiol-derivatized neoglycoconjugate, forming a 2D surface with maltose functionality. This self-assembled-monolayer-modified electrode was utilized for adsorption and spectroelectrochemical studies of tetraheme-containing type II cytochrome c3. The glycosylated surface allowed for the determination of the hemes' redox potentials and demonstrated enhanced spectroelectrochemical performance, in comparison to the widely used self-assembled monolayer of 11-mercapto-undecanoic acid.
Gomes
Inês
I
REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Di
Roberto E
RE
Pereira
Patrícia M
PM
Pereira
Inês A C
IA
Saraiva
Lígia M
LM
Penadés
Soledad
S
Franco
Ricardo
R
D000327:Adsorption; D001671:Biochemistry / Q000295:instrumentation* / Q000379:methods; D003574:Cytochrome c Group / Q000737:chemistry*; D003901:Desulfovibrio / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D005227:Fatty Acids / Q000737:chemistry; D006031:Glycosylation; D006418:Heme / Q000737:chemistry; D008024:Ligands; D008320:Maltose / Q000737:chemistry; D010084:Oxidation-Reduction; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties
C505222:11-mercaptoundecanoic acid; D003574:Cytochrome c Group; D005227:Fatty Acids; D008024:Ligands; D013438:Sulfhydryl Compounds; D006418:Heme; D008320:Maltose; C017165:cytochrome c(3)