Effects of enantiomer and isomer permeation enhancers on transdermal delivery of ligustrazine hydrochloride.
Enantiomers and isomers, such as D-limonene, L-limonene, and alpha-terpinene, were selected as enhancers. The effects and mechanisms of penetration enhancers on in vitro transdermal delivery of ligustrazine hydrochloride (LH) across hairless porcine dorsal skin were investigated. Transdermal fluxes of LH through porcine skin were determined in vitro by Franz-type diffusion cells. D-limonene, L-limonene, and alpha-terpinene could significantly promote the transdermal fluxes of LH, but no statistical difference (p > 0.05) between them was found. The lag time of L-limonene and alpha-terpinene were 2.55 and 2.20 times compared with that of D-limonene. Fourier transform-infrared (FTIR) was carried out to analyze the effects of enhancers on the biophysical natures of the stratum corneum (SC) and the permeation enhancement mechanism. FTIR spectra revealed that the changes of peak shift and peak area due to C-H stretching vibrations in the SC lipids were associated with the selected enhancers. All of them could perturb and extract the SC lipids to different extent and L-limonene showed obvious changes. Morphological changes of the skin treated with enhancers were monitored by a scanning electron microscope (SEM). The extraction of the SC lipids by the enhancers led to the disruption of SC and the desquamated SC flake. Apparent density (AD) was newly proposed to estimate the desquamated extent of SC flake. The results showed that the enantiomers and isomers enhanced the permeation of LH by pleiotropic mechanisms.
Zhang
Chun-feng
CF
School of Chinese Materia Medica, China Pharmaceutical University, Nanjing, China.
Yang
Zhong-lin
ZL
Luo
Jia-bo
JB
D000277:Adjuvants, Pharmaceutic / Q000493:pharmacokinetics*; D000279:Administration, Cutaneous; D000818:Animals; D053138:Cyclohexenes; D004337:Drug Carriers; D066298:In Vitro Techniques; D007641:Keratolytic Agents / Q000494:pharmacology; D000077222:Limonene; D008055:Lipids; D010539:Permeability; D011719:Pyrazines / Q000008:administration & dosage / Q000493:pharmacokinetics*; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013237:Stereoisomerism; D013552:Swine; D013729:Terpenes
D000277:Adjuvants, Pharmaceutic; D053138:Cyclohexenes; D004337:Drug Carriers; D007641:Keratolytic Agents; D008055:Lipids; D011719:Pyrazines; D013729:Terpenes; D000077222:Limonene; C017953:tetramethylpyrazine