[The isolated perfused porcine kidney model for investigations concerning surgical therapy procedures].
Experiments to develop innovative surgical therapy procedures are conventionally conducted on animals, as crucial aspects like tissue removal and bleeding disposition cannot be investigated in vitro. Extracorporeal organ models however reflect these aspects and could thus reduce the use of animals for this purpose fundamentally in the future. The aim of this work was to validate the isolated perfused porcine kidney model with regard to its use for surgical purposes on the basis of histological and radiological procedures. The results show that neither storage nor artificial perfusion led to any structural or functional damage which would affect the quality of the organ. The kidney model is highly suitable for simulating the main aspects of renal physiology and allows a constant calibration of perfusion pressure and tissue temperature. Thus, with only a moderate amount of work involved, the kidney model provides a cheap and readily available alternative to conventional animal experiments; it allows standardised experimental settings and provides valid results.
Peters
Kristina
K
Urologische Klinik, Universitaetsklinikum Mannheim, Fakultät fuer klinische Medizin Mannheim der Ruprecht-Karls-Universität Heidelberg, Deutschland. tina_peters@web.de
Michel
Maurice Stephan
MS
Matis
Ulrike
U
Häcker
Axel
A
D000818:Animals; D032984:Ethics, Research; D005858:Germany; D066298:In Vitro Techniques; D007668:Kidney / Q000601:surgery*; D008954:Models, Biological; D010477:Perfusion; D013552:Swine