Proteins modified with DNAzymes or aptamers act as biosensors or biosensor labels.
Hybrid systems composed of a glucose oxidase (GOx)/peroxidase-mimicking DNAzyme, and of microperoxidase-11 (MP-11)/anti-thrombin aptamer were synthesized. The hybrid systems were employed as amplifying labels for the colorimetric or chemiluminescence detection of an enzyme functions, and thrombin analysis, respectively. In the GOx/DNAzyme system, the GOx-mediated oxidation of glucose led to the formation of H(2)O(2), and this activated the oxidation of ABTS to a colored product, or to the generation of chemiluminescence in the presence of luminol. The MP-11/anti-thrombin aptamer enabled the amplified analysis of thrombin by the MP-11-mediated generation of chemiluminescence in the presence of luminol/H(2)O(2).
Shlyahovsky
Bella
B
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Li
Di
D
Katz
Eugenii
E
Willner
Itamar
I
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods; D021881:DNA, Catalytic / Q000737:chemistry*; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods; D010544:Peroxidases / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods; D013917:Thrombin / Q000032:analysis* / Q000737:chemistry
D052157:Aptamers, Nucleotide; D021881:DNA, Catalytic; D005949:Glucose Oxidase; D010544:Peroxidases; C021799:microperoxidase; D013917:Thrombin; D005947:Glucose