title: ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases.
abstract: The regulation of gene expression by estrogen receptor-alpha (ERalpha) requires the coordinated and temporal recruitment of diverse sets of transcriptional co-regulator complexes, which mediate nucleosome remodelling and histone modification. Using ERalpha as bait in a yeast two-hybrid screen, we have identified a novel ERalpha-interacting protein, ZNF366, which is a potent corepressor of ERalpha activity. The interaction between ZNF366 and ERalpha has been confirmed in vitro and in vivo, and is mediated by the zinc finger domains of the two proteins. Further, we show that ZNF366 acts as a corepressor by interacting with other known ERalpha corepressors, namely RIP140 and CtBP, to inhibit expression of estrogen-responsive genes in vivo. Together, our results indicate that ZNF366 may play an important role in regulating the expression of genes in response to estrogen.
authors.affiliation: Department of Oncology, Imperial College London, Du Cane Road, London W12 0NN, UK.
authors.forename: Jorge
authors.initials: J
authors.lastname: Lopez-Garcia
authors.forename: Manikandan
authors.initials: M
authors.lastname: Periyasamy
authors.forename: Ross S
authors.initials: RS
authors.lastname: Thomas
authors.forename: Mark
authors.initials: M
authors.lastname: Christian
authors.forename: Maria
authors.initials: M
authors.lastname: Leao
authors.forename: Parmjit
authors.initials: P
authors.lastname: Jat
authors.forename: Karin B
authors.initials: KB
authors.lastname: Kindle
authors.forename: David M
authors.initials: DM
authors.lastname: Heery
authors.forename: Malcolm G
authors.initials: MG
authors.lastname: Parker
authors.forename: Lakjaya
authors.initials: L
authors.lastname: Buluwela
authors.forename: Tahereh
authors.initials: T
authors.lastname: Kamalati
authors.forename: Simak
authors.initials: S
authors.lastname: Ali
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism; D019556:COS Cells; D002352:Carrier Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D000078723:Nuclear Receptor Interacting Protein 1; D012097:Repressor Proteins / Q000378:metabolism*; D014018:Tissue Distribution; D016335:Zinc Fingers
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D047628:Estrogen Receptor alpha; C000627127:NRIP1 protein, human; D009687:Nuclear Proteins; D000078723:Nuclear Receptor Interacting Protein 1; D012097:Repressor Proteins; C515832:ZNF366 protein, human; D000429:Alcohol Oxidoreductases; C096794:C-terminal binding protein; D006655:Histone Deacetylases