Synthesis, characterization, and utility of thermoresponsive natural/unnatural product macroligands for affinity chromatography.
[Structure: see text] The synthesis and characterization of thermoresponsive, water-soluble poly-N-isopropyl acrylamide (PNIPAM) derived macroligands displaying cyclosporin A (CsA) and dexamethasone (Dex) for use as novel affinity resins are described. Characterization of these soluble macroligands, including ligand loading and integrity, was determined by 1H NMR spectroscopy. One of the CsA macroligands was used in a protein affinity experiment to capture known binding proteins of CsA, the cyclophilins, from Jurkat T-cell lysates.
Zhou
Min
M
Department of Chemistry, Texas A&M University, P.O. Box 30012, College Station, Texas 77842-3012, USA.
Sivaramakrishnan
Ananthapadmanab
A
Ponnamperuma
Krishan
K
Low
Woon-Kai
WK
Li
Chunmei
C
Liu
Jun O
JO
Bergbreiter
David E
DE
Romo
Daniel
D
D002846:Chromatography, Affinity / Q000379:methods*; D021983:Cyclophilins; D016572:Cyclosporine / Q000737:chemistry*; D003907:Dexamethasone / Q000737:chemistry*; D005938:Glucocorticoids / Q000737:chemistry; D006801:Humans; D007166:Immunosuppressive Agents / Q000737:chemistry; D019169:Jurkat Cells; D008024:Ligands; D015394:Molecular Structure; D011485:Protein Binding
D005938:Glucocorticoids; D007166:Immunosuppressive Agents; D008024:Ligands; D003907:Dexamethasone; D016572:Cyclosporine; D021983:Cyclophilins