title: Cloning and overexpression of ribosomal protein L39 gene from deltamethrin-resistant Culex pipiens pallens.
abstract: The complete sequence of ribosomal protein L39 has been cloned from deltamethrin-resistant strain of Culex pipiens pallens (DR1 strain). Quantitative RT-PCR analysis indicated that the RPL39 transcription level was 23.4 times higher in DR1 strain than in susceptible strain at 4th instar larvae. The RPL39 expression was also found to be consistently higher throughout the life cycle of DR1 strain. A protein of predicted size 17 kDa has been detected by Western blotting in RPL39-transfected mosquito C6/36 cells. These RPL39-transfected cells also showed enhanced deltamethrin resistance compared to plasmid vector-transfected cells as determined by methyl tritiated thymidine ((3)H-TdR) incorporation. These results indicate that RPL39 is expressed at higher levels in DR1 strain, and may confer some insecticide resistance in Cx. pipiens pallens.
authors.affiliation: Department of Pathogenic Biology, Nanjing Medical University, Nanjing, Jiangsu Province, PR China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Tan
authors.forename: Lixin
authors.initials: L
authors.lastname: Sun
authors.forename: Donghui
authors.initials: D
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Jin
authors.initials: J
authors.lastname: Qian
authors.forename: Xiaobang
authors.initials: X
authors.lastname: Hu
authors.forename: Weijie
authors.initials: W
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.forename: Changliang
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D003465:Culex / Q000737:chemistry / Q000187:drug effects / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009570:Nitriles / Q000494:pharmacology*; D010802:Phylogeny; D011722:Pyrethrins / Q000494:pharmacology*; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D014162:Transfection
chemical_list: D000199:Actins; D018076:DNA, Complementary; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D012269:Ribosomal Proteins; C040223:ribosomal protein L39; C017180:decamethrin; D012313:RNA