title: Stereoselective determination of benalaxyl in plasma by chiral high-performance liquid chromatography with diode array detector and application to pharmacokinetic study in rabbits.
abstract: A chiral high-performance liquid chromatography method with diode array detector was developed and validated for stereoselective determination of benalaxyl (BX) in rabbit plasma. Good separation was achieved at 20 degrees C using cellulose tris-(3,5-dimethylphenylcarbamate) as chiral stationary phase, a mixture of n-hexane and 2-propanol (97:3) as mobile phase at a flow rate of 1.0 ml/min. The assay method was linear over a range of concentrations (0.25-25 microg/ml) in plasma and the mean recovery was greater than 90% for both enantiomers. The limits of quantification and detection for both enantiomers in plasma were 0.25 and 0.1 microg/ml, respectively. Intra- and interday relative standard deviations (RSDs) did not exceed 10% for three-tested concentrations. The method was successfully applied to pharmacokinetic studies of BX enantiomers in rabbits. The result suggested that the pharmacokinetics of BX enantiomers was stereoselective in rabbits.
authors.affiliation: Department of Applied Chemistry, China Agricultural University, Beijing 100094, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qiu
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Wang
authors.forename: Wentao
authors.initials: W
authors.lastname: Zhu
authors.forename: Guifang
authors.initials: G
authors.lastname: Jia
authors.forename: Xinquan
authors.initials: X
authors.lastname: Wang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D011817:Rabbits; D013237:Stereoisomerism
chemical_list: C052866:benalaxyl; D000409:Alanine