title: Rotavirus impairs the biosynthesis of brush-border-associated dipeptidyl peptidase IV in human enterocyte-like Caco-2/TC7 cells.
abstract: Rotavirus is the leading cause of severe dehydrating diarrhoea in infants and young children worldwide. This virus infects mature enterocytes in the small intestine, and induces structural and functional damage. In the present study, we have identified a new mechanism by which rotavirus impairs a brush border-associated intestinal protein. We show that infection of enterocyte-like Caco-2/TC7 cells by rhesus monkey rotavirus (RRV) impairs the biosynthesis of dipeptidyl peptidase IV (DPP IV), an important hydrolase in the digestion of dietary proline-rich proteins. We show that the enzyme activity of DPP IV was reduced, and that rearrangements of the protein occurred at the apical domain of the RRV-infected cells. Using pulse-chase experiments and cell surface immunoprecipitation, we have demonstrated that RRV infection did not affect the stability or apical targeting of DPP IV, but did induce a dramatic decrease in its biosynthesis. Using quantitative RT-PCR, we showed that RRV had no effect on the level of expression of DPP IV mRNA, suggesting that the observed decrease in the biosynthesis of the protein is related to an effect of the virus at the translational level.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Université Paris XI, UMR-S 756, Signalisation et Physiopathologie des Cellules Epithéliales, Faculté de Pharmacie, Châtenay-Malabry, F-92296 France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Beau
authors.forename: Arnaud
authors.initials: A
authors.lastname: Berger
authors.forename: Alain L
authors.initials: AL
authors.lastname: Servin
mesh_terms: D015153:Blotting, Western; D018938:Caco-2 Cells; D002462:Cell Membrane / Q000378:metabolism; D018819:Dipeptidyl Peptidase 4 / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D020895:Enterocytes / Q000166:cytology / Q000201:enzymology* / Q000821:virology; D004789:Enzyme Activation; D004795:Enzyme Stability; D019084:Fluorescent Antibody Technique, Indirect; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D047468:Immunoprecipitation; D008871:Microvilli / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000254:growth & development*
chemical_list: D012333:RNA, Messenger; D018819:Dipeptidyl Peptidase 4