title: [Effects of sodium arsenite on catalase in human keratinocytes].
abstract: OBJECTIVE
  To evaluate the effects of sodium arsenite on the activity, the mRNA and the protein expression of CAT in human keratinocyte cell line (HaCaT).
  
  
  METHODS
  The activity of catalase (CAT) was detected by ultraviolet direct velocity assay. RT-PCR was used to detect the mRNA expression of CAT and Western blotting was conducted to detect the protein expression of CAT.
  
  
  RESULTS
  If the cells were treated with higher than 5.0 micromol/L sodium arsenite, the activity, mRNA and protein expression of CAT were decreased significantly and in a dosage dependent fashion (P < 0.05).
  
  
  CONCLUSIONS
  CAT is inhibited by sodium arsenite in the transcription, translation and activity levels.
authors.affiliation: Hygiene Department, Colleges of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116027, China.
authors.forename: Xian-ce
authors.initials: XC
authors.lastname: Sun
authors.forename: Feng-yuan
authors.initials: FY
authors.lastname: Piao
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Xu
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Jin
authors.forename: Gui-fan
authors.initials: GF
authors.lastname: Sun
mesh_terms: D018053:Arsenites / Q000633:toxicity*; D015153:Blotting, Western; D002374:Catalase / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017670:Sodium Compounds / Q000633:toxicity*
chemical_list: D018053:Arsenites; D012333:RNA, Messenger; D017670:Sodium Compounds; C017947:sodium arsenite; D002374:Catalase