In vivo high-speed imaging of individual cells in fast blood flow.
In vivo, label-free, high-speed (up to 10,000 with the potential for 40,000 frames per second), high-resolution (up to 300 nm) real-time continuous imaging with successive framing of circulating individual erythrocytes, leukocytes, and platelets in fast blood flow is developed. This technique, used in an animal model, reveals the extremely high dynamic deformability of erythrocytes in natural flow. Potential applications of this technique are discussed with focus on time-resolved monitoring of the cell deformation dynamics in the native biological environment, which may have diagnostic value for the early diagnosis of diseases.
Zharov
Vladimir P
VP
Philips Classic Laser Biomedical Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205-7199, USA.
Galanzha
Ekaterina I
EI
Menyaev
Yulian
Y
Tuchin
Valery V
VV
D000818:Animals; D001773:Blood Cells / Q000166:cytology; D001783:Blood Flow Velocity / Q000502:physiology; D004912:Erythrocytes / Q000166:cytology*; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods*; D008297:Male; D008638:Mesenteric Arteries / Q000166:cytology*; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted