Cloning, chromosomal localization and expression patterns of porcine Kruppel-like factors 4, -5, -7 and the early growth response factor 2.
Kruppel-like factors (KLF) and the early growth response factor 2 (EGR2) are important zinc finger transcription factors in vertebrates. We have cloned the full length coding sequence (CDS) of porcine KLF5, KLF7 and EGR2, which are 1374, 909 and 1416 bp, respectively. KLF4, KLF5 and EGR2 were then chromosomally mapped to porcine 1q28-29, 11q13-14 and 14q23-25, respectively. Moreover, the tissue expression patterns of KLF4, KLF5, KLF7 and EGR2 imply their probable roles in specific tissues. This is the first report of the basic study of these zinc finger transcription factors in pigs; this information will be helpful for future functional studies.
Yang
H W
HW
Laboratory of Biochemistry, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
Xia
T
T
Chen
Z L
ZL
Feng
S Q
SQ
Peng
Y
Y
Zhou
L
L
Gan
L
L
Yang
Z Q
ZQ
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D051767:Early Growth Response Protein 2 / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D009928:Organ Specificity; D013045:Species Specificity; D013552:Swine / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
D051767:Early Growth Response Protein 2; C497328:Egr2 protein, rat; C000715469:Klf4 protein, rat; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors