title: Distribution of NMDA receptor subunit NR1 in arctic ground squirrel central nervous system.
abstract: Hibernation is a natural model of neuroprotection and adult synaptic plasticity. NMDA receptors (NMDAR), which play key roles in excitotoxicity and synaptic plasticity, have not been characterized in a hibernating species. Tolerance to excitotoxicity and cognitive enhancement in Arctic ground squirrels (AGS, Spermophilus parryii) suggests that NMDAR expression may decrease in hibernation and increase upon arousal. NMDAR consist of at least one NMDAR1 (NR1) subunit, which is required for receptor function. Localization of NR1 reflects localization of the majority, if not all, NMDAR complexes. The purpose of this study, therefore, was to characterize the distribution of NR1 subunits in AGS central nervous system using immunohistochemistry. In addition, we compare NR1 expression in hippocampus of hibernating AGS (hAGS) and inter-bout euthermic AGS (ibeAGS) and assess changes in cell somata size using NR1 stained sections in three hippocampal sub-regions (CA1, CA3, and dentate gyrus). For the first time, we report that immunoreactivity of anti-NR1 is widely distributed throughout the central nervous system in AGS and is similar to other species. No differences exist in the expression and distribution of NR1 in hAGS and ibeAGS. However, we report a significant decrease in size of hippocampal CA1 and dentate gyrus NR1-expressing neuronal somata during hibernation torpor.
authors.affiliation: Alaskan Basic Neuroscience Program, Institute of Arctic Biology, University of Alaska, Fairbanks, AK 99775, USA. huzhao@ucsd.edu
authors.forename: Huiwen W
authors.initials: HW
authors.lastname: Zhao
authors.forename: Sherri L
authors.initials: SL
authors.lastname: Christian
authors.forename: Marina R
authors.initials: MR
authors.lastname: Castillo
authors.forename: Abel
authors.initials: A
authors.lastname: Bult-Ito
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Drew
mesh_terms: D000818:Animals; D001831:Body Temperature / Q000502:physiology; D001931:Brain Mapping; D048429:Cell Size; D002490:Central Nervous System / Q000033:anatomy & histology / Q000378:metabolism*; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism*; D006605:Hibernation / Q000502:physiology*; D006624:Hippocampus / Q000033:anatomy & histology / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009498:Neurotoxins / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D012589:Sciuridae / Q000033:anatomy & histology / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: C409466:NR1 NMDA receptor; D009498:Neurotoxins; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid