title: [Subtype and sequence analysis of the ENV gene of HIV-1 drug users in Guangdong Pearl River delta.].
abstract: BACKGROUND
  To determine the subtype of HIV-1 of drug users (DUS) in Guangdong Pearl River delta.
  
  
  METHODS
  HIV-1 pro-viral DNA from buffy coat of 43 DUS in Guangdong was amplified by nested PCR. The C2-V3 regions of HIV-1 ENV gene was sequenced directly from the PCR product and analyzed.
  
  
  RESULTS
  The 43 DUS were confirmed to be infected with four HIV-1 subtype or Circulating Recombinant Form (CRFs): 07-BC(n=29), AE(n=9), 08-BC(n=3) and B(n=2). Genetic distances showed that the AE group was the closest to CM240 strain isolated in Thailand which is mainly circulating in sexually transmitted infector. The 07-BC group was the closest to C54A strain isolated in Northeastern China. The 08-BC group was the closest to 97CNGX-9F strain isolated in Guangxi, China. The B strain was the closest to rl42 strain isolated in Thailand.
  
  
  CONCLUSIONS
  HIV-1 CRFs 07-BC predominates in DUS in Guangdong Pearl River delta.
authors.affiliation: Center for Diseases Control and Prevention of Guangdong Province, Guangzhou 510300, China.
authors.forename: J
authors.initials: J
authors.lastname: Yan
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: P F
authors.initials: PF
authors.lastname: Ma
authors.forename: P
authors.initials: P
authors.lastname: Lin
authors.forename: H
authors.initials: H
authors.lastname: Xing
authors.forename: L M
authors.initials: LM
authors.lastname: Diao
authors.forename: Y M
authors.initials: YM
authors.lastname: Shao
mesh_terms: D001483:Base Sequence; D002681:China; D055030:Drug Users; D015752:Genes, env; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D045483:Rivers; D017421:Sequence Analysis