Response of interleukin-1beta in the magnocellular system to salt-loading.
Drinking 2% NaCl decreases interleukin (IL)-1beta in the neural lobe and enhances IL-1 Type 1 receptor expression in magnocellular neurones and pituicytes. To quantify cytokine depletion from the neural lobe during progressive salt loading and determine whether the changes are reversible and correspond with stores of vasopressin (VP) or oxytocin (OT), rats were given water on day 0 and then 2% NaCl to drink for 2, 5, 8 or 5 days followed by 5 days of water (rehydration). Control rats drinking only water were pair-fed amounts eaten by 5-day salt-loaded animals. Animals were decapitated on day 8, the neural lobe frozen and plasma hormones analysed by radioimmunoassay (OT, VP) or enzyme-linked immunosorbent assay (IL-1beta). IL-1beta, VP and OT in homogenates of the neural lobe were quantified by immunocapillary electrophoresis with laser-induced fluorescence detection. Differences were determined by ANOVA, Tukey's t-test, Dunnett's procedure, Fisher's least significant difference and linear regression analysis. In response to salt-loading, rats lost body weight similar to pair-fed controls, drank progressively more 2% NaCl and excreted greater urine volumes. Plasma VP increased at days 2 and 8 of salt-loading, whereas osmolality, OT and cytokine were enhanced after 8 days with IL-1beta remaining elevated after rehydration. In the neural lobe, all three peptides decreased progressively with increasing duration of salt-loading (IL-1beta, r2 = 0.98; OT, r2 = 0.94; VP, r2 = 0.93), beginning on day 2 (IL-1beta; VP) or 5 (OT), with only VP replenished by rehydration. IL-1beta declined more closely (P < 0.0001; ANOVA interaction analysis) with OT (r2 = 0.96) than VP (r2 = 0.86), indicative of corelease from the neural lobe during chronic dehydration. Local effects of IL-1beta on magnocellular terminals, pituicytes and microglia in the neural lobe with activation of forebrain osmoregulatory structures by circulating cytokine may sustain neurosecretion of OT and VP during prolonged salt-loading.
Summy-Long
J Y
JY
Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA. jsl2@psu.edu
Hu
S
S
Pruss
A
A
Chen
X
X
Phillips
T M
TM
D000704:Analysis of Variance; D000818:Animals; D003681:Dehydration / Q000097:blood / Q000139:chemically induced; D053583:Interleukin-1beta / Q000097:blood*; D008297:Male; D009994:Osmolar Concentration; D010121:Oxytocin / Q000097:blood*; D010904:Pituitary Gland, Posterior / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010909:Pituitary Hormones, Posterior / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D017673:Sodium Chloride, Dietary; D018709:Statistics, Nonparametric; D014667:Vasopressins / Q000097:blood*; D014882:Water-Electrolyte Balance / Q000187:drug effects / Q000502:physiology*
D053583:Interleukin-1beta; D010909:Pituitary Hormones, Posterior; D017673:Sodium Chloride, Dietary; D014667:Vasopressins; D010121:Oxytocin