title: Differential cell division history between neutrophils and macrophages in their development from granulocyte-macrophage progenitors.
abstract: The appearance of monocytes before neutrophils in the blood during haematopoietic recovery in myelosuppressive patients is commonly observed, thus suggesting a difference in the cell division history between these two lineages in the differentiation from granulocyte-macrophage (GM) progenitors. We investigated the cell division histories of murine GM progenitors. When analysed by the dye dilution method, GM progenitors gave rise to Gr-1+Fms+ and Gr-1+Fms- cells that passed through similar rounds of cell division during initial 5 d of culture. The Gr-1+Fms+ cells showed morphological features of monocytes, while Gr-1+Fms- cells exhibited an immature morphology of neutrophils. In the subsequent culture, a decline in the number of Gr-1+Fms+ cells was observed, while Gr-1+Fms- cells increased. The proliferation of Gr-1+Fms- cells and no cell division of Gr-1+Fms+ cells were confirmed by DNA staining, Ki-67 expression, membrane dye staining and bromodeoxyuridine incorporation. These Gr-1+Fms- cells acquired mature neutrophil morphology, whereas Gr-1+Fms+ cells became macrophages. These results demonstrate that GM progenitors generate postmitotic monocytes earlier than mature neutrophils. Our data may also offer one explanation for the rapid recovery of monocytes in comparison with neutrophils in the early phase of haematopoietic regeneration.
authors.affiliation: Department of Haematology and Oncology, Mie University Graduate School of Medicine, Mie, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Sugimoto
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Katayama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Masuya
authors.forename: Eri
authors.initials: E
authors.lastname: Miyata
authors.forename: Masaya
authors.initials: M
authors.lastname: Ueno
authors.forename: Kohshi
authors.initials: K
authors.lastname: Ohishi
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nishii
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takakura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiku
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000032:analysis; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D016130:Immunophenotyping / Q000379:methods; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000166:cytology*
chemical_list: D039481:CD11b Antigen; D003470:Culture Media; D016207:Cytokines