Homocysteine increases neuronal damage in hippocampal slices receiving oxygen and glucose deprivation.
Homocystinuria is an inherited metabolic disorder caused by severe deficiency of cystationine beta-synthase activity, resulting in the tissue accumulation of homocysteine (Hcy). Affected patients usually present many signs and symptoms such as seizures, mental retardation, atherosclerosis and stroke. The aim of this study is to evaluate in vivo and in vitro effects of Hcy using hippocampal slices from Wistar rats exposed to oxygen and glucose deprivation (OGD), followed by reoxygenation, an in vitro model of hypoxic-ischemic events. Neural cell injury was quantified by the measurement of lactate dehydrogenase (LDH) released from damaged cells into the extracellular fluid. The results showed that both in vivo and in vitro Hcy increased the LDH released to de incubation medium, suggesting an increase of tissue damage caused by OGD. This fact can be related with the high incidence of stroke in homocystinuric patients.
Tagliari
Bárbara
B
Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil.
Zamin
Lauren L
LL
Salbego
Christianne G
CG
Netto
Carlos Alexandre
CA
Wyse
Angela T S
AT
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D016923:Cell Death; D005947:Glucose / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006710:Homocysteine / Q000097:blood / Q000494:pharmacology*; D006712:Homocystinuria / Q000378:metabolism* / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009410:Nerve Degeneration / Q000378:metabolism* / Q000473:pathology; D009924:Organ Culture Techniques; D010100:Oxygen / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology
D006710:Homocysteine; D007770:L-Lactate Dehydrogenase; D005947:Glucose; D010100:Oxygen