title: Trefoil factor 2 in gland mucous cell mucin in the mucous gel covering normal or damaged gastric mucosa using the Mongolian gerbil model.
abstract: OBJECTIVE
  Trefoil factor 2 (TFF2) is localized in gastric gland mucous cells. The purpose of the study was to determine whether TFF2 and gastric mucin are localized in mucous cells and in the surface mucous gel layer (SMGL) of the normal gastric mucosa or in the mucoid cap adherent to gastric mucosal lesions in Mongolian gerbils.
  
  
  METHODS
  Gastric mucosal lesions were induced in Mongolian gerbils using oral administration of Helicobacter pylori (H. pylori), subcutaneous administration of indomethacin, or oral administration of 30% ethanol. Tissue samples were fixed in Carnoy's solution for preservation of the SMGL, dehydrated, and embedded in paraffin. Histochemical staining for gastric mucins and immunostaining for TFF2 were performed.
  
  
  RESULTS
  It was found that surface mucous cell mucin and gland mucous cell mucin were segregated in the SMGL covering the normal gastric mucosa, and the mucin of the mucoid cap covering the mucosal lesions was primarily gland mucous cell mucin. There was a co-localization of TFF2 in gland mucous cell mucin in gland mucous cells, the SMGL, and the mucoid cap.
  
  
  CONCLUSIONS
  The co-localization of TFF2 in gland mucous cells and in the adherent mucus suggests a physical interaction between TFF2 and gland mucous cell mucin, and the participation of TFF2 trapped in the adherent mucus functions in mucosal defense, healing, and repair.
authors.affiliation: Department of Surgery, School of Medicine, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Hayama
authors.forename: Michitaka
authors.initials: M
authors.lastname: Nakamura
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Fukuto
authors.initials: F
authors.lastname: Maruta
authors.forename: Shinichi
authors.initials: S
authors.lastname: Miyagawa
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ota
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D000431:Ethanol / Q000494:pharmacology; D005752:Gastric Mucins / Q000032:analysis*; D005753:Gastric Mucosa / Q000737:chemistry* / Q000166:cytology / Q000187:drug effects; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000378:metabolism; D016480:Helicobacter pylori; D006651:Histocytochemistry; D007150:Immunohistochemistry; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D010455:Peptides / Q000032:analysis*; D000071164:Trefoil Factor-2
chemical_list: D005752:Gastric Mucins; D010455:Peptides; D000071164:Trefoil Factor-2; D000431:Ethanol; D007213:Indomethacin