title: Elevated sister chromatid exchange frequencies in dividing human peripheral blood lymphocytes exposed to 50 Hz magnetic fields.
abstract: The in vitro cytomolecular technique, sister chromatid exchange (SCE), was applied to test the clastogenic potentiality of extremely low frequency (ELF) electromagnetic fields (EMFs) on human peripheral blood lymphocytes (HPBLs). SCE frequencies were scored in dividing peripheral blood lymphocytes (PBLs) from six healthy male blood donors in two rounds of experiments, R1 and R2, to determine reproducibility. Lymphocyte cultures in the eight experiments conducted in each round were exposed to 50 Hz sinusoidal (continuous or pulsed) or square (continuous or pulsed) MFs at field strengths of 1 microT or 1 mT for 72 h. A significant increase in the number of SCEs/cell in the grouped experimental conditions compared to the controls was observed in both rounds. The highest SCE frequency in R1 was 10.03 for a square continuous field, and 10.39 for a square continuous field was the second highest frequency in R2. DNA crosslinking at the replication fork is proposed as a model which could explain the mechanistic link between ELF EMF exposure and increased SCE frequency.
authors.affiliation: Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand.
authors.forename: M A
authors.initials: MA
authors.lastname: Wahab
authors.forename: J V
authors.initials: JV
authors.lastname: Podd
authors.forename: B I
authors.initials: BI
authors.lastname: Rapley
authors.forename: R E
authors.initials: RE
authors.lastname: Rowland
mesh_terms: D001973:Bromodeoxyuridine / Q000378:metabolism; D004261:DNA Replication; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D012854:Sister Chromatid Exchange
chemical_list: D001973:Bromodeoxyuridine