An experimental, non-uremic rabbit model of peritoneal dialysis.
Peritoneal dialysis (PD) is a well established method of depuration in uremic patients. Standard dialysis solutions currently in use are not biocompatible with the peritoneal membrane. Studying effects of dialysate on peritoneal membrane in humans is still a challenge. There is no consensus on the ideal experimental model so far. We, therefore, wanted to develop a new experimental non-uremic rabbit model of peritoneal dialysis, which would be practical, easy to conduct, not too costly, and convenient to investigate the long-term effect of dialysis fluids. The study was done on 17 healthy Chinchilla male and female rabbits, anesthetized with Thiopental in a dose of 0.5 mg/kg body mass. A catheter, specially made from Tro-soluset (Troge Medical GMBH, Hamburg, Germany) infusion system, was then surgically inserted and tunneled from animals' abdomen to their neck. The planned experimental procedure was 4 weeks of peritoneal dialysate instillation. The presented non-uremic rabbit model of peritoneal dialysis is relatively inexpensive, does not require sophisticated technology and was well tolerated by the animals. Complications such as peritonitis, dialysis fluid leakage, constipation and catheter obstruction were negligible. This model is reproducible and can be used to analyze the effects of different dialysis solutions on the rabbit peritoneal membrane.
Zunic-Bozinovski
S
S
Institute of Pathophysiology, School of Medicine, University of Belgrade, Serbia. nzunic@ptt.yu
Lausevic
Z
Z
Krstic
S
S
Jovanovic
N
N
Trbojevic-Stankovic
J
J
Stojimirovic
B
B
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000494:pharmacology; D002408:Catheters, Indwelling; D015314:Dialysis Solutions / Q000009:adverse effects*; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D010530:Peritoneal Dialysis / Q000379:methods*; D010532:Peritoneal Diseases / Q000139:chemically induced / Q000517:prevention & control; D010537:Peritoneum / Q000187:drug effects* / Q000648:ultrastructure; D011817:Rabbits; D016896:Treatment Outcome; D014511:Uremia / Q000628:therapy*
D001672:Biocompatible Materials; D015314:Dialysis Solutions