title: Isolation of protoplast from aerial mycelia and fruit-body formation from regenerated mycelia in Pleurotus cornucopiae.
abstract: We have developed a simple and efficient method for protoplast isolation from Pleurotus cornucopiae. Protoplasts were isolated from aerial mycelia cultured on potato dextrose agar medium without time-consuming propagation in liquid culture. Protoplast yield was significantly increased by means of a decompressing pretreatment of mycelia in enzyme solution and a subsequent enzyme reaction with vibrational mixing. The isolated protoplasts regenerated mycelia and these mycelia formed fruit-bodies without any morphological abnormalities.
authors.affiliation: Department of Biology, Faculty of Science and Engineering, Konan University, Okamoto, Higashinada-ku, Kobe, Japan.
authors.forename: Masahide
authors.initials: M
authors.lastname: Mizoguchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kitano
authors.forename: Wataru
authors.initials: W
authors.lastname: Takahashi
authors.forename: Fuyuki
authors.initials: F
authors.lastname: Sugawara
authors.forename: Osamu
authors.initials: O
authors.lastname: Tanaka
mesh_terms: D020076:Pleurotus / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism*; D014732:Vibration