Penicillin acylase catalysis in the presence of ionic liquids.
Several ionic liquids were used as reaction media for penicillin G acylase catalysis. In all the assayed ionic liquids, [bmim]PF6 proved good media for PGA-catalyzed hydrolysis. A novel [bmim]PF6/water two-phase system is provided for 6-aminopenicillanic acid (APA) production, which will be more benefical than aquous batch systems used widely in industrial production of APA.
Zhang
Wei-Guo
WG
State Key Laboratory of Bioreactor Engineering, Institute of New World Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
Wei
Dong-Zhi
DZ
Yang
Xue-Peng
XP
Song
Qing-Xun
QX
D002384:Catalysis; D004789:Enzyme Activation; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology*; D006868:Hydrolysis; D052578:Ionic Liquids / Q000737:chemistry*; D010399:Penicillin Amidase / Q000737:chemistry*; D010400:Penicillin G / Q000737:chemistry*; D013379:Substrate Specificity
D052578:Ionic Liquids; D010399:Penicillin Amidase; D010400:Penicillin G