Crystallization of Ranasmurfin, a blue-coloured protein from Polypedates leucomystax.
Ranasmurfin, a previously uncharacterized approximately 13 kDa blue protein found in the nests of the frog Polypedates leucomystax, has been purified and crystallized. The crystals are an intense blue colour and diffract to 1.51 A with P2(1) symmetry and unit-cell parameters a = 40.9, b = 59.9, c = 45.0 A, beta = 93.3 degrees . Self-rotation function analysis indicates the presence of a dimer in the asymmetric unit. Biochemical data suggest that the blue colour of the protein is related to dimer formation. Sequence data for the protein are incomplete, but thus far have identified no model for molecular replacement. A fluorescence scan shows a peak at 9.676 keV, indicating that the protein binds zinc and suggesting a route for structure solution.
McMahon
Stephen A
SA
Centre for Biomolecular Science and The Scottish Structural Proteomics Facility, The University of St Andrews Fife KY16 9RH, Scotland, UK.
Walsh
Martin A
MA
Ching
Rosalind Tan Yan
RT
Carter
Lester G
LG
Dorward
Mark
M
Johnson
Kenneth A
KA
Liu
Huanting
H
Oke
Muse
M
Bloch
Carlos
C
Kennedy
Malcolm W
MW
Latiff
Aishah A
AA
Cooper
Alan
A
Taylor
Garry L
GL
White
Malcolm F
MF
Naismith
James H
JH
D000818:Animals; D001001:Anura; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation
D002352:Carrier Proteins