Biocompatible superparamagnetic iron oxide nanoparticle dispersions stabilized with poly(ethylene glycol)-oligo(aspartic acid) hybrids.
Methoxypoly(ethylene glycol)-oligo(aspartic acid) (MPEG-Asp(n)-NH(2), n = 2-5) hybrid block copolymers were synthesized and used as stabilizers to prepare superparamagnetic Fe(3)O(4) nanoparticles with magnetite as the inner core and and poly(ethylene glycol) as the hydrophilic outer shell. The aqueous dispersions of the nanoparticles were stable at pH 2-11 and in 1M NaCl solution, when repeat number, n, was 3 or more. Transmission electron microscopy showed that the nanoparticles, stabilized with MPEG-Asp(3)-NH(2), were about 14 nm in diameter. Magnetic measurements indicated that MPEG-Asp(3)-NH(2)-coated iron oxide nanoparticles showed superparamagnetic behavior. Cell adhesion assay and in vitro cell viability/cytotoxicity studies showed that MPEG-Asp(3)-NH(2)-coated iron oxide nanoparticles had less effect on cell adhesion/viability and morphology, and less cytotoxicity compared with uncoated, poly (acrylic acid)-coated, and MPEG-poly(acrylic acid)-coated iron oxide nanoparticles.
Wan
Shourong
S
Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China.
Huang
Junsheng
J
Guo
Miao
M
Zhang
Hongkai
H
Cao
Youjia
Y
Yan
Husheng
H
Liu
Keliang
K
D000818:Animals; D001224:Aspartic Acid; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D020099:Coated Materials, Biocompatible; D003911:Dextrans; D052203:Ferrosoferric Oxide; D007501:Iron; D058185:Magnetite Nanoparticles; D008422:Materials Testing; D051379:Mice; D053758:Nanoparticles / Q000648:ultrastructure; D049329:Nanostructures; D010087:Oxides; D010316:Particle Size; D011092:Polyethylene Glycols
D020099:Coated Materials, Biocompatible; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; D001224:Aspartic Acid; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide