Repeated methamphetamine administration alters expression of the NMDA receptor channel epsilon2 subunit and kinesins in the mouse brain.
Repeated amphetamine administration results in behavioral sensitization. Behavioral sensitization related to abuse and/or relapse may be associated with stable changes in gene expression. To explore the participating genes, we examined the changes in gene expression levels 24 h or 21 days (long-term withdrawal period) after chronic methamphetamine (METH) treatment for 2 weeks. The expression of several genes related to glutamatergic neural transmission was altered, although changes in the corresponding protein expression were not always consistent with the results for mRNA expression. Of interest, in the frontal cortex of mice treated with METH for 2 weeks, protein expression levels of KIF17 and the N-methyl-D-asparate (NMDA) receptor channel epsilon2 subunit (NRepsilon2) were concomitantly increased. The alteration in expression of these proteins, KIF17 and NRepsilon2, might be a part of the molecular basis of the behavioral sensitization to METH.
Yamamoto
Hideko
H
Division of Psychobiology, Tokyo Institute of Psychiatry, Tokyo 156-8585, Japan. yamahide@prit.go.jp
Imai
Kazuhide
K
Kamegaya
Etsuko
E
Takamatsu
Yukio
Y
Irago
Masashi
M
Hagino
Yoko
Y
Kasai
Sinya
S
Shimada
Kiyo
K
Yamamoto
Toshifumi
T
Sora
Ichiro
I
Koga
Hisashi
H
Ikeda
Kazutaka
K
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D015259:Dopamine Agents / Q000494:pharmacology*; D016547:Kinesins / Q000378:metabolism*; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*
D015259:Dopamine Agents; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D008694:Methamphetamine; D016547:Kinesins