Neuroglobin and cytoglobin overexpression protects human SH-SY5Y neuroblastoma cells against oxidative stress-induced cell death.
Although reactive oxygen species (ROS) at physiological concentrations are required for normal cell function, excessive production of ROS is detrimental to cells. Neuroglobin and cytoglobin are two globins, whose functions are still a matter of debate. A potential role in the detoxification of ROS is suggested. The influence of neuroglobin and cytoglobin on cell death after oxidative stress in human neuroblastoma SH-SY5Y cells was evaluated. Exposure of SH-SY5Y cells to paraquat or H(2)O(2) resulted in a concentration- and time-dependent induction of apoptotic and necrotic cell death. H(2)O(2) was 16 times more potent to induce cell death as compared to paraquat. SH-SY5Y cells transfected with plasmid DNA containing the neuroglobin or cytoglobin sequence showed enhanced survival after exposure to 300 microM H(2)O(2) for 24h as compared to untransfected controls. This finding suggests that neuroglobin and cytoglobin protect SH-SY5Y cells against oxidative stress-induced cell death.
Fordel
Elke
E
Department of Biomedical Sciences, University of Antwerp, Belgium.
Thijs
Liesbet
L
Martinet
Wim
W
Lenjou
Marc
M
Laufs
Tilmann
T
Van Bockstaele
Dirk
D
Moens
Luc
L
Dewilde
Sylvia
S
D015153:Blotting, Western / Q000379:methods; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D000078842:Cytoglobin; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000379:methods; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology*; D005914:Globins / Q000378:metabolism*; D006540:Herbicides / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009447:Neuroblastoma; D000078787:Neuroglobin; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010269:Paraquat / Q000494:pharmacology; D013997:Time Factors
C000627265:CYGB protein, human; D000078842:Cytoglobin; D006540:Herbicides; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D016877:Oxidants; D005914:Globins; D006861:Hydrogen Peroxide; D010269:Paraquat