Inactivation of a European strain of West Nile virus in single- donor platelet concentrate using the INTERCEPT blood system.
OBJECTIVE
In order to prevent West Nile virus (WNV) contaminations by transfusion, the French National Blood Service decided to evaluate the INTERCEPT Blood System's efficiency on a European strain.


METHODS
Culture supernatant of WNV was used to infect six platelets concentrates. Viral titre was determined by plaque reduction neutralization test before and after viral inactivation using the INTERCEPT Blood System.


RESULTS
In all assays, the absence of plaque forming unit was observed after viral inactivation. The log reduction observed ranged between > 5.1 logs to > 5.2 logs.


CONCLUSIONS
INTERCEPT Blood System is a commercially viral inactivation method potentially useful in order to prevent WNV transmission by blood products in France during re-emerging outbreaks.
Gallian
P
P
Etablissement Français du Sang Alpes-Méditerranée, Marseille, France. pierre.gallian@efs.sante.fr
Vignoli
C
C
Dombey
A M
AM
Mayaudon
V
V
Lin
L
L
Galichet
V
V
Cantaloube
J F
JF
De Micco
P
P
D001782:Blood Donors; D001792:Blood Platelets / Q000821:virology*; D004196:Disease Outbreaks / Q000517:prevention & control; D018562:Disease Transmission, Infectious / Q000517:prevention & control*; D005060:Europe; D005602:France; D006801:Humans; D017713:Platelet Transfusion / Q000009:adverse effects; D010948:Viral Plaque Assay / Q000379:methods* / Q000592:standards; D038301:Virus Inactivation; D014902:West Nile virus