[Expression of selected genes involved in transport of ions in papillary thyroid carcinoma].
BACKGROUND
The studies of papillary thyroid cancer (PTC) gene expression profile have shown changes in expression of genes involved in transport of several ions. The aim of our study was a real-time PCR evaluation of three of them: KCNJ2, SLC4A4 and SLC34A2.


METHODS
The analysis was carried out in PTC tumors and normal thyroid samples gained from 38 patients. Real-time quantitative PCR (Q-PCR) was performed (Taqman) with beta-glucuronidase (GUS) as the reference gene.


RESULTS
We observed 20 x increase of SLC34A2 expression in PTC. This gene encodes Na+/PO(4) (3-) cotransporter. Considerable increase of gene expression has been shown also for KCNJ2, encoding a potassium ion channel. SLC4A4, which encodes the Na+/HCO(3) (2-) cotransporter, exhibited a 7-fold decrease in PTC.


CONCLUSIONS
The performed study revealed that SLC34A2 gene exhibited the most distinct change in expression and may become a molecular marker of papillary thyroid cancer.
Gałeza-Kulik
Monika
M
Department of Nuclear Medicine and Endocrine Oncology, M. Sklodowska-Curie Cancer Center and Institute of Oncology, Gliwice Branch,Wybrzeże Armii Krajowej 15, Gliwice.
Zebracka
Jadwiga
J
Szpak-Ulczok
Sylwia
S
Czarniecka
Aleksandra Krawczyk Agnieszka
AK
Kukulska
Aleksandra
A
Gubała
Elzbieta
E
Stojcev
Zoran
Z
Wiench
Małgorzata
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002277:Carcinoma; D002291:Carcinoma, Papillary; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb / Q000235:genetics*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics*
D014408:Biomarkers, Tumor; C493557:SLC34A2 protein, human; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb