title: Reagentless ultrasensitive specific DNA array detection based on responsive polymeric biochips.
abstract: Self-assembled molecular structures immobilized on solid substrates and composed of fluorophore-tagged oligonucleotide probes and an optical polymeric transducer were investigated for the trace level detection of DNA target molecules. Rapid and efficient energy transfer between the polymeric transducer and fluorophores within the molecular aggregates leads to a massive intrinsic amplification of the fluorescence signal and to the label-free detection of as little as 300 DNA molecules, with the specificity required for the detection of single-nucleotide mismatches. This capacity for attomolar detection levels while the sensing structures are attached onto solid supports could lead to the development of biochip platforms for fast and simple PCR-free multitarget DNA detection.
authors.affiliation: Department of Chemistry and Centre de Recherches sur la Science et L'ingénierie des Macromolécules (CERSIM), Université Laval, Québec, Canada G1K 7P4.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Najari
authors.forename: Hoang Anh
authors.initials: HA
authors.lastname: Ho
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Gravel
authors.forename: Philippe
authors.initials: P
authors.lastname: Nobert
authors.forename: Denis
authors.initials: D
authors.lastname: Boudreau
authors.forename: Mario
authors.initials: M
authors.lastname: Leclerc
mesh_terms: D020137:Base Pair Mismatch / Q000235:genetics; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000032:analysis* / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D004247:DNA