Elucidation of carotenoid patterns in citrus products by means of comprehensive normal-phase x reversed-phase liquid chromatography.
A novel approach for carotenoid analysis has been developed. Orange essential oil and juice carotenoids were separated by means of comprehensive dual-gradient elution HPLC, using normal phase with a microbore silica column in the first dimension (first D), reversed phase with a monolithic C18 column in the second dimension (second D), and a 10-port switching valve as an interface. An on-line photodiode array detector was used in order to obtain absorption spectra. Peak identification was obtained by combining retention data with the UV-visible spectra.
Dugo
Paola
P
Dipartimento di Scienze degli Alimenti e dell'Ambiente, Facoltà di Scienze MM.FF.NN., Università di Messina, Salita Sperone, 98166 Messina, Italy.
Skeriková
Veronika
V
Kumm
Tiina
T
Trozzi
Alessandra
A
Jandera
Pavel
P
Mondello
Luigi
L
D001628:Beverages / Q000032:analysis*; D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D002338:Carotenoids; D012822:Silicon Dioxide