title: Rapid expression of Mycobacterium avium subsp. paratuberculosis recombinant proteins for antigen discovery.
abstract: Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease, a chronic granulomatous enteritis of ruminants and other species. Detection of infection in animals is hampered by the lack of sensitive and specific diagnostic assays. We describe here an approach that utilizes translationally active PCR fragments for the rapid in vitro transcription and translation of recombinant proteins for antigen discovery in M. avium subsp. paratuberculosis. The investigations showed that the MAP1272c protein selectively reacts with sera from Johne's disease-positive cattle and represents an antigen of potential utility in M. avium subsp. paratuberculosis immunodiagnostics.
authors.affiliation: Department of Microbiology, University of Minnesota, 1500 Gortner Ave., St. Paul, MN 55108, USA.
authors.forename: Lingling
authors.initials: L
authors.lastname: Li
authors.forename: Shirin
authors.initials: S
authors.lastname: Munir
authors.forename: John P
authors.initials: JP
authors.lastname: Bannantine
authors.forename: Srinand
authors.initials: S
authors.lastname: Sreevatsan
authors.forename: Sagarika
authors.initials: S
authors.lastname: Kanjilal
authors.forename: Vivek
authors.initials: V
authors.lastname: Kapur
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology / Q000378:metabolism*; D010283:Paratuberculosis / Q000175:diagnosis; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D011993:Recombinant Fusion Proteins