Effects of 50 Hz sinusoidal magnetic fields on Hsp27, Hsp70, Hsp90 expression in porcine aortic endothelial cells (PAEC).
The aim of the present study was to investigate the influence of 50 Hz sinusoidal magnetic field on Hsp27, Hsp70, and Hsp90 expression in a model of primary culture of porcine aortic endothelial cells (PAEC). We took into consideration the Hsp profile in terms of mRNA expression, protein expression and protein localization inside the cells. The choice of the cell system was motivated by the involvement of the endothelial cells in the onset of many diseases; moreover, only few reports describe the effects of extremely low frequency magnetic fields (ELF-MFs) on such cells. ELF-MF exposure induced an increase in the mRNA levels of the three proteins, which was statistically significant for Hsp70. On the contrary, we did not observe any influence on Hsp27, Hsp70, and Hsp90 protein levels. Analysis in situ by immunofluorescence revealed that ELF-MF exposure affected the cellular distribution of Hsp27; in particular a partial relocalization in the nucleus was observed.
Bernardini
Chiara
C
Department of Veterinary Morphophysiology and Animal Production DIMORFIPA, Bologna University, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy. cbernardini@vet.unibo.it
Zannoni
Augusta
A
Turba
Maria Elena
ME
Bacci
Maria Laura
ML
Forni
Monica
M
Mesirca
Pietro
P
Remondini
Daniel
D
Castellani
Gastone
G
Bersani
Ferdinando
F
D000818:Animals; D002478:Cells, Cultured; D004574:Electromagnetic Fields / Q000009:adverse effects*; D004730:Endothelium, Vascular / Q000166:cytology / Q000528:radiation effects*; D015870:Gene Expression / Q000528:radiation effects; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000528:radiation effects*; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis / Q000528:radiation effects*; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000528:radiation effects*; D012333:RNA, Messenger / Q000378:metabolism / Q000528:radiation effects; D034421:Sus scrofa
D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger