GABA-induced intracellular Ca2+ elevation in the embryonic chick brainstem slice.
We examined the intracellular Ca2+ ([Ca2+]i) elevation evoked by GABA in an 8-day embryonic chick brainstem slice using a Ca imaging technique with Ca green-1 AM. When small quantities of GABA were pressure-ejected on the surface of the slice, the [Ca2+]i elevation was clearly detected. The GABA-induced [Ca2+]i elevation was eliminated in a Ca2+-free solution, whereas the previously reported GABA-induced light-scattering change was independent of extracellular Ca2+. Although, micro-application of glycine or glutamate also induced [Ca2+]i elevation, these changes were smaller than that by GABA. These results suggest that the GABA-induced [Ca2+]i elevation is due to Ca2+ entry resulting from membrane depolarization and may play an important role in the development of the central nervous system (CNS).
Momose-Sato
Yoko
Y
Department of Physiology, Tokyo Medical and Dental University Graduate School and Faculty of Medicine, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
Kinoshita
Masae
M
Sato
Katsushige
K
D000818:Animals; D001933:Brain Stem / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002642:Chick Embryo; D045604:Extracellular Fluid / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D005680:gamma-Aminobutyric Acid; D002118:Calcium