Development of an enzyme-linked immunosorbent assay for quantitative determination of quizalofop-p-ethyl.
Accurate quantification of quizalofop-p-ethyl is essential for it may do harm to humans and animals through both water and food. Currently, detection of quizalofop-p-ethyl mainly relies on methods such as gas chromatography, high performance liquid chromatography, and gas chromatography-mass spectrometry. Although these techniques are reliable, they are relatively expensive and time-consuming because of multistep sample cleanup. To address this, we developed a competitive indirect enzyme-linked immunosorbent assay (ciELISA) with a polyclonal antibody against quizalofop-p-ethyl that was generated in our lab. The IC(50) of detection was 0.03495 microg/mL, and the lowest detection limit reached 0.00192 microg/mL. Furthermore, the method had high specificity for it did not cross-react with other structure-related compounds. When water and soil samples that were fortified with quizalofop-p-ethyl were analyzed by this ELISA, recoveries were in the range of 89-110% from water and 81-108% from soil. Good correlations between this immunoassay and gas chromatography data were obtained for residues of quizalofop-p-ethyl in water and soil. Our data indicate that this method is a convenient analytical technique for monitoring quizalofop-p-ethyl in waters without extraction and the extra cleanup step and in soil without the cleanup step.
Zeng
Deyi
D
Department of Pesticide, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Shi
Haiyan
H
Li
Bo
B
Wang
Minghua
M
Song
Baoan
B
D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006540:Herbicides / Q000032:analysis*; D011422:Propionates / Q000032:analysis*; D011810:Quinoxalines / Q000032:analysis*; D011817:Rabbits; D014874:Water Pollutants, Chemical / Q000032:analysis*
D006540:Herbicides; D011422:Propionates; D011810:Quinoxalines; D014874:Water Pollutants, Chemical; C064835:quizalofop-ethyl