title: Human hepatic cytochrome p450-specific metabolism of parathion and chlorpyrifos.
abstract: Organophosphorus pesticides (OPs) remain a potential concern to human health because of their continuing worldwide use. Thiophosphorus OPs, once bioactivated by cytochromes P450 (P450s), form oxon metabolites, which are potent acetylcholinesterase inhibitors. This study investigated the rate of desulfation (activation) and dearylation (detoxification) of parathion and chlorpyrifos in human liver microsomes. In addition, recombinant human P450s were used to quantify, for the first time, the P450-specific kinetic variables (K(m) and V(max)) for each compound for future use in refining human physiologically based pharmacokinetic/pharmacodynamic (PBPK/PD) models of OP exposure. CYP1A2, 2B6, 2C9, 2C19, 3A4, 3A5, and 3A7 were found to be active to a widely varying degree in parathion metabolism, whereas all, with the exception of CYP2C9, were also found to be active in chlorpyrifos metabolism. CYP2B6 and CYP2C19 demonstrated low K(m) and high V(max) values for the metabolism of both model compounds, which supports their role as the primary enzymes that regulate metabolism at low-level human exposures to OPs. With K(m) and V(max) values of 0.61 microM, 4827 pmol/min/nmol P450 and 0.81 microM, 12,544 pmol/min/nmol for formation of paraoxon and chlorpyrifos-oxon, respectively, CYP2B6 favored the desulfation reaction. CYP2C19 activity favored dearylation with K(m) and V(max) values of 0.60 microM, 2338 pmol/min/nmol P450 and 1.63 microM, 13,128 pmol/min/nmol for formation of p-nitrophenol and 3,4,5-tricholorpyrindinol, respectively. P450-specific kinetic parameters for OP metabolism will be used with age-dependent hepatic P450 content to enhance PBPK/PD models so that OP exposures can be modeled to protect human health in different age groups.
authors.affiliation: University at Buffalo, State University of New York, Department of Pharmacology and Toxicology, Buffalo, New York, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Foxenberg
authors.forename: Barbara P
authors.initials: BP
authors.lastname: McGarrigle
authors.forename: James B
authors.initials: JB
authors.lastname: Knaak
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Kostyniak
authors.forename: James R
authors.initials: JR
authors.lastname: Olson
mesh_terms: D004390:Chlorpyrifos / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006801:Humans; D007306:Insecticides / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D010278:Parathion / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D007306:Insecticides; D011994:Recombinant Proteins; D010278:Parathion; D003577:Cytochrome P-450 Enzyme System; D004390:Chlorpyrifos