Quantitative genetic analysis in Saccharomyces cerevisiae using epistatic miniarray profiles (E-MAPs) and its application to chromatin functions.
The use of the budding yeast Saccharomyces cerevisiae as a simple eukaryotic model system for the study of chromatin assembly and regulation has allowed rapid discovery of genes that influence this complex process. The functions of many of the proteins encoded by these genes have not yet been fully characterized. Here, we describe a high-throughput methodology that can be used to illuminate gene function and discuss its application to a set of genes involved in the creation, maintenance and remodeling of chromatin structure. Our technique, termed E-MAPs, involves the generation of quantitative genetic interaction maps that reveal the function and organization of cellular proteins and networks.
Schuldiner
M
M
Department of Cellular and Molecular Pharmacology, The California Institute for Quantitative Biomedical Research, UCSF, Mission Bay Campus, Byers Hall, 1700 4th Street, San Francisco, California 94143-2540, USA. mschuldiner@cmp.ucsf.edu
Collins
S R
SR
Weissman
J S
JS
Krogan
N J
NJ
D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D019295:Computational Biology; D004843:Epistasis, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D025941:Protein Interaction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
D002843:Chromatin; D029701:Saccharomyces cerevisiae Proteins