title: Functional genomics of histone modification and non-histone chromosomal proteins using the polytene chromosomes of Drosophila.
abstract: Knowing the genomic distribution of chromosomal proteins and of histone modifications provides essential insight into function. The giant polytene chromosomes of the Drosophila larval salivary glands provide a high-resolution genomic map with a resolution exceeded only by chromatin immunoprecipitation. Immunofluorescence localization of chromosomal proteins and specific post-translational modifications of histones is a simple and rapid tool for the functional genomics of chromosomal proteins.
authors.affiliation: Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, MO 63104, USA. eissenjc@slu.edu
authors.forename: Joel C
authors.initials: JC
authors.lastname: Eissenberg
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics; D023281:Genomics / Q000379:methods*; D006657:Histones / Q000378:metabolism*; D012469:Salivary Glands / Q000378:metabolism
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; D006657:Histones