title: Infusion of hypertonic saline (7.5%) does not change neutrophil oxidative burst or expression of endothelial adhesion molecules after abdominal hysterectomy.
abstract: BACKGROUND
  Previous studies found hypertonicity to affect neutrophils in intact laboratory animals and in human blood cell cultures. We investigated whether infusion of hypertonic saline in a clinical relevant dose before hysterectomy affected peripheral blood neutrophils and their response to surgery.
  
  
  METHODS
  Fifteen women scheduled for open abdominal hysterectomy were randomized double-blindly to infusion of 4 mL/kg 7.5% NaCl, 4 mL/kg 0.9% NaCl, or 32 mL/kg 0.9% NaCl over 20 minutes. Blood was collected at baseline, after infusion, 1, 4, and 24 hours postoperatively for the determination of leukocyte and differential count, neutrophil membrane expression of endothelial adhesion molecules by flow cytometry, and O2- -generation by superoxide dismutase-inhibitable reduction of cytochrome C.
  
  
  RESULTS
  Surgery induced well-known changes in the number and distribution of white blood cells, reduced the expression of adhesion molecules, and halved the superoxide production unrelated to the tonicity or volume of the infused fluids.
  
  
  CONCLUSIONS
  Infusion of a clinically relevant dose of hypertonic saline has no detectable effect on the membrane expression of endothelial adhesion molecules or O2- -generation in circulating neutrophils after elective abdominal hysterectomy.
authors.affiliation: Department of Anesthesia and Intensive Care, Viborg County Hospital, Viborg, DK. jaakp@dadlnet.dk
authors.forename: Jens Aage
authors.initials: JA
authors.lastname: Kolsen-Petersen
authors.forename: Torsten Bøgh
authors.initials: TB
authors.lastname: Rasmussen
authors.forename: Jan
authors.initials: J
authors.lastname: Krog
authors.forename: Marianne
authors.initials: M
authors.lastname: Hokland
authors.forename: Else Kirstine
authors.initials: EK
authors.lastname: Tonnesen
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D015703:Antigens, CD / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004311:Double-Blind Method; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007044:Hysterectomy / Q000379:methods; D016023:Integrins / Q000096:biosynthesis*; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D009994:Osmolar Concentration; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage*; D019087:Selectins / Q000378:metabolism*; D012964:Sodium / Q000097:blood; D013481:Superoxides / Q000378:metabolism*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D006454:Hemoglobins; D016023:Integrins; D012462:Saline Solution, Hypertonic; D019087:Selectins; D019010:Vascular Cell Adhesion Molecule-1; D013481:Superoxides; D012964:Sodium