Selective breeding for catalepsy changes the distribution of microsatellite D13Mit76 alleles linked to the 5-HT serotonin receptor gene in mice.
Catalepsy (pronounced motor inhibition) is a natural defensive reaction against predator. Recently, the quantitative trait locus for catalepsy was mapped on mouse chromosome 13 near the 5-HT(1A) serotonin receptor gene. Here, the linkage between catalepsy and the 5-HT(1A) receptor gene was verified using breeding experiment. Selective breeding for high predisposition to catalepsy was started from backcross BC[CBA x (CBA x AKR)] generation between catalepsy-prone (CBA) and catalepsy-resistant (AKR) mouse strains. CBA and AKR strains also differed in the 5-HT(1A) receptor functional activity. A rapid increase of cataleptic percentage from 21.2% in the backcrosses to 71% in the third generation of selective breeding (S3) was shown. The fragment of chromosome 13 including the 5-HT(1A) receptor gene was marked with D13Mit76 microsatellite. Breeding for catalepsy increased the concentration of CBA-derived and decreased the concentration of AKR-derived alleles of microsatellite D13Mit76 in the S1 and S2. All mice of the S9 and S12 were homozygous for CBA-derived allele of D13Mit76 marker. Mice of the S12 showed CBA-like receptor activity. These findings indicate that selective breeding for behavior can involve selection of polymorphic variants of the 5-HT(1A) receptor gene.
Kondaurova
E M
EM
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
Bazovkina
D V
DV
Kulikov
A V
AV
Popova
N K
NK
D000818:Animals; D002375:Catalepsy / Q000235:genetics*; D002874:Chromosome Mapping; D004195:Disease Models, Animal; D046352:Freezing Reaction, Cataleptic / Q000502:physiology; D008040:Genetic Linkage; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006824:Hybridization, Genetic; D007178:Inbreeding; D051379:Mice; D008806:Mice, Inbred AKR; D008808:Mice, Inbred CBA; D018895:Microsatellite Repeats; D044282:Receptor, Serotonin, 5-HT1A / Q000235:genetics*; D013045:Species Specificity
D005819:Genetic Markers; D044282:Receptor, Serotonin, 5-HT1A