title: Conformational organization of the 3' untranslated region in the tomato bushy stunt virus genome.
abstract: The 3' untranslated regions (UTRs) of positive-strand RNA viruses often form complex structures that facilitate various viral processes. We have examined the RNA conformation of the 352 nucleotide (nt) long 3' UTR of the Tomato bushy stunt virus (TBSV) genome with the goal of defining both local and global structures that are important for virus viability. Gel mobility analyses of a 3'-terminal 81 nt segment of the 3' UTR revealed that it is able to form a compact RNA domain (or closed conformation) that is stabilized by a previously proposed tertiary interaction. RNA-RNA gel shift assays were used to provide the first physical evidence for the formation of this tertiary interaction and revealed that it represents the dominant or "default" structure in the TBSV genome. Further analysis showed that the tertiary interaction involves five base pairs, each of which contributes differently to overall complex stability. Just upstream from the 3'-terminal domain, a long-distance RNA-RNA interaction involving 3' UTR sequences was found to be required for efficient viral RNA accumulation in vivo and to also contribute to the formation of the 3'-terminal domain in vitro. Collectively, these results provide a comprehensive overview of the conformational and functional organization of the 3' UTR of the TBSV genome.
authors.affiliation: Department of Biology, York University, Toronto, Ontario, Canada M3J 1P3.
authors.forename: Hong
authors.initials: H
authors.lastname: Na
authors.forename: Marc R
authors.initials: MR
authors.lastname: Fabian
authors.forename: K Andrew
authors.initials: KA
authors.lastname: White
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics; D038621:Regulatory Sequences, Ribonucleic Acid; D017864:Tombusvirus / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral; D038621:Regulatory Sequences, Ribonucleic Acid