[Beneficial effects of human umbilical cord blood mononuclear cells transplantation on rats with experimental myocardial infarction].
OBJECTIVE
Human umbilical cord blood contains abundant immature stem/progenitor cells, which may contribute to the repair of infarcted myocardium. Present study aimed to explore the feasibility and effects of human umbilical cord blood mononuclear cells (HUCBC) transplantation for the treatment of myocardial infarction.


METHODS
Forty-five male Wistar rats were randomly divided into three groups: (1) Myocardial infarction (MI plus vehicle, n = 15), (2) MI plus cell transplantation (HUCBC were implanted into the peri-infarct area immediately after MI, n = 15), (3) Normal control group (n = 15). After echocardiography and hemodynamic measurements, the rats were sacrificed for histological and immunochemical examinations one month post MI.


RESULTS
The transplanted HUCBC survived and participated the repair process in host heart. Significantly improved left ventricular function was evidenced by echocardiography in cell transplantation group compared to the MI control group. Left ventricular end-diastolic pressure was significantly reduced LVEDP (21.08 +/- 8.10) vs (30.82 +/- 9.59) mm Hg, P < 0.05], +dp/dt(max) [(4.29 +/- 1.27) vs (3.24 +/- 0.75) mm Hg/ms, P < 0.05] and -dp/dt(max) increased [(3.71 +/- 0.79) vs (3.00 +/- 0.49) mm Hg/ms, P < 0.05] in cell transplantation rats compared with MI control rats. vWF immunostaining examination showed significantly increased microvessels within the boundary of infarcted myocardium in cell transplantation group compared to the MI control group (P < 0.01).


CONCLUSIONS
HUCBC transplantation may improve cardiac function in MI rats by promoting microvessel formation.
Hu
Cheng-heng
CH
Department of Cardiology, First Affiliated Hospital, SUN Yat-sen University, Guangzhou 510080, China.
Wu
Gui-fu
GF
Wang
Xiao-qing
XQ
Yang
Yan-hua
YH
He
Xiao-hong
XH
Du
Zhi-min
ZM
D000818:Animals; D036101:Cord Blood Stem Cell Transplantation; D006801:Humans; D008297:Male; D009203:Myocardial Infarction / Q000473:pathology / Q000601:surgery*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar