Quantum dot optical encoded polystyrene beads for DNA detection.
A novel multiplex analysis technology based on quantum dot (QD) optical encoded beads was studied. Carboxyl functionalized polystyrene beads, about 100 microm in size, were precisely encoded by the various ratios of two types of QDs whose emission wavelengths are 576 and 628 nm, respectively. Then the different encoded beads were covalently immobilized with different probes in the existing of sulfo-NHS and 1-[3-(Dimethylamino) propyl]-3-ethylcarbodiimide methiodide, and the probe density could reach to 3.1 mmol/g. These probe-linked encoded beads were used to detect the target DNA sequences in complex DNA solution by hybridization. Hybridization was visualized using fluorescein isothiocynate-labeled DNA sequences. The results show that the QDs and target signals can be obviously identified from a single-bead-level spectrum. This technology can detect DNA targets effectively with a detection limit of 0.2 microg/mL in complex solution.
Cao
Yuan-Cheng
YC
Key Laboratory of Biomedical Photonics of Ministry of Education, HuBei Bioinformatics and Molecular Imaging Key Laboratory, Huazhong University of Science and Technology, College of Life Science and Technology, Wuhan, HuBei, China 430074.
Liu
Tian-Cai
TC
Hua
Xiao-Feng
XF
Zhu
Xiao-Xia
XX
Wang
Hai-Qiao
HQ
Huang
Zhen-Li
ZL
Zhao
Yuan-Di
YD
Liu
Man-Xi
MX
Luo
Qing-Ming
QM
D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods*; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D011137:Polystyrenes / Q000737:chemistry*; D045663:Quantum Dots; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods
D011137:Polystyrenes; D004247:DNA