title: Detection of galectin-3 by novel peptidic photoprobes.
abstract: Photoprobes were prepared with specificity for binding, labeling, and visualizing galectin-3 in a mixture of proteins. The probes were derived from a galectin-3 binding 15-mer peptide sequence in which a benzophenone photolabel was incorporated at the N-terminus and in another case as a phenyl alanine replacement in the middle of the sequence. Detection of galectin-3 was possible in Escherichia coli lysates that were spiked with various amounts of galectin-3.
authors.affiliation: Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80082, 3508 TB Utrecht, The Netherlands.
authors.forename: Monique
authors.initials: M
authors.lastname: van Scherpenzeel
authors.forename: Martin
authors.initials: M
authors.lastname: van der Pot
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Arnusch
authors.forename: Rob M J
authors.initials: RM
authors.lastname: Liskamp
authors.forename: Roland J
authors.initials: RJ
authors.lastname: Pieters
mesh_terms: D001577:Benzophenones / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000737:chemistry; D037502:Galectin 3 / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D010777:Photochemistry
chemical_list: D001577:Benzophenones; D037502:Galectin 3; D007202:Indicators and Reagents; D010455:Peptides