title: Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the regulator AcrR from Escherichia coli.
abstract: This paper describes the cloning, expression, purification and preliminary X-ray data analysis of the AcrR regulatory protein. The Escherichia coli AcrR is a member of the TetR family of transcriptional regulators. It regulates the expression of the AcrAB multidrug transporter. Recombinant AcrR with a 6xHis tag at the C-terminus was expressed in E. coli and purified by metal-affinity chromatography. The protein was crystallized using hanging-drop vapor diffusion. X-ray diffraction data were collected from cryocooled crystals at a synchrotron light source. The best crystal diffracted to 2.5 A. The space group was determined to be P3(2), with unit-cell parameters a = b = 46.61, c = 166.16 A.
authors.affiliation: Department of Physics and Astronomy, Iowa State University, Ames, IA 50011, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Xi
authors.initials: X
authors.lastname: Qiu
authors.forename: Chih-Chia
authors.initials: CC
authors.lastname: Su
authors.forename: Feng
authors.initials: F
authors.lastname: Long
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Gu
authors.forename: Gerry
authors.initials: G
authors.lastname: McDermott
authors.forename: Edward W
authors.initials: EW
authors.lastname: Yu
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: C515217:AcrR protein, E coli; D017931:DNA Primers; D029968:Escherichia coli Proteins; D012097:Repressor Proteins