title: O-Acetylation in the O-specific polysaccharide isolated from Shigella flexneri serotype 2a.
abstract: Shigella flexneri causes diarrheal diseases especially in infants and children in developing countries. Modifications of the lipopolysaccharide (LPS) molecule, like bacteriophage-mediated glucosylation and acetylation of the O-specific chain (O-SP), are important for the LPS antigenicity and consequently for the immunogenicity of the polysaccharide-based vaccines against shigellosis. Here, we report the degree of O-acetylation and the localisation of O-acetyl groups and side-chain glucose substitution in the O-SP (scheme) in different preparations of S. flexneri type 2a LPS. [structure: see text]
authors.affiliation: National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA. kielbj@mail.nih.gov
authors.forename: Joanna
authors.initials: J
authors.lastname: Kubler-Kielb
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
authors.forename: Chiayung
authors.initials: C
authors.lastname: Chu
authors.forename: Rachel
authors.initials: R
authors.lastname: Schneerson
mesh_terms: D000107:Acetylation; D002240:Carbohydrate Sequence; D006031:Glycosylation; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D012763:Shigella flexneri / Q000737:chemistry*
chemical_list: D019081:O Antigens