A cellular FRET-based sensor for beta-O-GlcNAc, a dynamic carbohydrate modification involved in signaling.
beta-O-N-Acetyl-d-glucosamine (O-GlcNAc) is a dynamic carbohydrate modification that is involved in cell signaling and has been implicated in a variety of disease states, including Alzheimer's and type-II diabetes. Despite the importance of this modification, little is known about the spatial and temporal localization of O-GlcNAc during signaling. This is due to the lack of methods for the study of O-GlcNAc in living cell systems. Herein we report the first genetically encoded FRET-based sensor for the detection of O-GlcNAc dynamics in live mammalian cells.
Carrillo
Luz D
LD
Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station, A5300 Austin, TX 78712-0265, USA.
Krishnamoorthy
Lakshmipriya
L
Mahal
Lara K
LK
D000117:Acetylglucosamine / Q000032:analysis*; D015153:Blotting, Western; D002241:Carbohydrates / Q000138:chemical synthesis*; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation*; D006367:HeLa Cells; D006801:Humans; D015398:Signal Transduction
D002241:Carbohydrates; D000117:Acetylglucosamine