title: Activity and mode of action against fungal phytopathogens of bovine lactoferricin-derived peptides.
abstract: OBJECTIVE
  To evaluate the activity against fungal phytopathogens of two synthetic peptides derived from the protein bovine lactoferricin: the antibacterial active core of six amino acid residues (LfcinB(20-25)) and an extension of 15 amino acids (LfcinB(17-31)).
  
  
  RESULTS
  In vitro activity against fungal pathogens was determined and compared with that against model micro-organisms. Activity was demonstrated against fungi of agronomic relevance. Distinct antimicrobial properties in vitro were found for the two peptides. LfcinB(17-31) had growth inhibitory activity higher than LfcinB(20-25). However, LfcinB(17-31) was not fungicidal to quiescent conidia of Penicillium digitatum at the concentrations assayed, while LfcinB(20-25) killed conidia more efficiently. Microscopical observations showed that the mycelium of P. digitatum treated with LfcinB(17-31) developed alterations of growth, sporulation and chitin deposition, and permeation of hyphal cells. In experimental inoculations of mandarins, both peptides showed limited protective effect against the disease caused by P. digitatum.
  
  
  CONCLUSIONS
  LfcinB(20-25) and LfcinB(17-31) peptides were shown to have antimicrobial activity against plant pathogenic filamentous fungi, with distinct properties and mode of action.
  
  
  CONCLUSIONS
  LfcinB(20-25) and LfcinB(17-31) peptides offer novel alternatives to develop resistant plants by molecular breeding.
authors.affiliation: Departamento de Ciencia de los Alimentos, Instituto de Agroquímica y Tecnología de Alimentos (IATA) - CSIC, Valencia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Muñoz
authors.forename: J F
authors.initials: JF
authors.lastname: Marcos
mesh_terms: D000818:Animals; D002417:Cattle; D002957:Citrus / Q000382:microbiology; D007781:Lactoferrin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010407:Penicillium / Q000187:drug effects* / Q000648:ultrastructure; D010446:Peptide Fragments / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology; D010944:Plants / Q000382:microbiology*
chemical_list: D010446:Peptide Fragments; C082171:lactoferricin B; D007781:Lactoferrin