Comparison of hydroxylated print additives on antibody microarray performance.
Various hydroxylated additives were added to antibody print buffers at different concentrations to stabilize printed antibodies during normal array spot desiccation on commercial polymer-coated microarray slides. Polyvinyl alcohol addition to print buffers produced the most regular spot morphologies, homogeneous intra-spot antibody distribution, uniform fluorescence intensity, and improved analyte capture activity, maintained up to 1 month at 4 degrees C for capturing model analytes, anti-human IL-1beta, IL-4, and TNFalpha, on these microarraying slides.
Wu
Peng
P
Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523-1872, USA.
Grainger
David W
DW
D000906:Antibodies / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D019921:Biotinylation; D006801:Humans; D006900:Hydroxylation; D007091:Image Processing, Computer-Assisted; D053583:Interleukin-1beta; D015847:Interleukin-4 / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D011487:Protein Conformation; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D000906:Antibodies; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4