Pyrethroid-resistant diamondback moth expresses alternatively spliced sodium channel transcripts with and without T929I mutation.
This study revealed two distinct alternatively spliced exons, A1 and A2, encoding part of the domain IIS4-IIS5 of the para-sodium channel gene in the diamondback moth (DBM). Exons A1 and A2, respectively, revealed 79% and 91% identity at the nucleotide and amino acid levels. Both alternative exons included the T929I site, which has been associated with pyrethroid resistance in DBM. In the pyrethroid-resistant strain, susceptible (Thr) and resistant (Ile) amino acids were encoded at the T929I site in exons A1 and A2, respectively, but in the pyrethroid-susceptible strain, only Thr was encoded at the site in both exons. The transcripts containing exon A1 were expressed constitutively in all developmental stages. The transcripts containing exon A2 were also detected in all developmental stages, but the levels were significantly lower in the 3rd and 4th instar larvae. Tissue-specific data from the 4th instar larvae and adults showed that the expression of transcripts containing exon A2 was higher in heads than in bodies. These findings suggest that alternative splicing of the para-sodium channel gene might produce distinct channels with different sensitivities to pyrethroids, possibly in a tissue-specific manner.
Sonoda
Shoji
S
Research Institute for Bioresources, Okayama University; Kurashiki, Okayama 710-0046, Japan. sonodas@rib.okayama-u.ac.jp
Igaki
Chikako
C
Ashfaq
Muhammad
M
Tsumuki
Hisaaki
H
D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology; D008969:Molecular Sequence Data; D009036:Moths / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D009154:Mutation / Q000235:genetics*; D026023:Permethrin / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*; D015222:Sodium Channels / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D019476:Insect Proteins; D007306:Insecticides; D011722:Pyrethrins; D015222:Sodium Channels; D026023:Permethrin