Enhanced activity of 3alpha-hydroxysteroid dehydrogenase by addition of the co-solvent 1-butyl-3-methylimidazolium (L)-lactate in aqueous phase of biphasic systems for reductive production of steroids.
The enzyme activity of 3alpha-hydrosteroid dehydrogenase (HSDH) was enhanced by the addition of the co-solvent 1-butyl-3-methylimidazolium (L)-lactate ([Bmim][lactate]) to 50 mM Tris-HCl buffer. When utilizing [Bmim][lactate], the reaction velocity of HSDH increased. Also, reductive production of androsterone was investigated in an aqueous-organic solvent biphasic system containing 5% [Bmim][lactate] as the co-solvent of aqueous phase. In a coupled-enzyme system comprising HSDH and formate dehydrogenase (FDH), a two-fold increase in production rate of androsterone was obtained when utilizing [Bmim][lactate] with NADH regeneration.
Okochi
Mina
M
Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Nakagawa
Izumi
I
Kobayashi
Takeshi
T
Hayashi
Shuhei
S
Furusaki
Shintaro
S
Honda
Hiroyuki
H
D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific) / Q000737:chemistry*; D000738:Androsterone / Q000138:chemical synthesis*; D005560:Formate Dehydrogenases / Q000737:chemistry; D006913:Hydroxysteroid Dehydrogenases / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry; D009243:NAD / Q000737:chemistry*
D052578:Ionic Liquids; D009243:NAD; D019344:Lactic Acid; D000738:Androsterone; D006913:Hydroxysteroid Dehydrogenases; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific); D005560:Formate Dehydrogenases