Influence of the two-component system SaeRS on global gene expression in two different Staphylococcus aureus strains.
The two-component system SaeRS consisting of the histidin kinase SaeS and the response regulator SaeR is known to act on virulence gene expression in Staphylococcus aureus. In order to get a more comprehensive picture on SaeR-regulated genes, we studied the contribution of the two-component system on global gene expression by using both the proteomic and transcriptomic approach. Altogether, a loss of SaeRS resulted in a decreased amount of at least 17 extracellular proteins and two cell surface-associated proteins, among them several important virulence factors such as HlgA, HlgB, HlgC, LukF, and LukM. SaeRS activates the expression of these genes at the transcriptional level. The amount of the five proteins Aur, SspA, SsaA, Plc, and GlpQ was negatively influenced by SaeRS. However, the transcription of the corresponding genes was not affected by the two-component system. SaeRS had also no measurable influence on the transcription of the regulatory genes agr, sarA, arlRS, and sigB that contribute to the regulation of SaeRS-dependent virulence factors identified in this investigation. Our results clearly show that SaeRS is strongly involved in the tight temporal control of virulence factor expression in S. aureus. Its precise role within the regulatory network remains to be determined.
Rogasch
Kathrin
K
Institut für Mikrobiologie, Ernst-Moritz-Arndt-Universität, F.-L.-Jahn-Strasse 15, D-17487 Greifswald, Germany.
Rühmling
Vanessa
V
Pané-Farré
Jan
J
Höper
Dirk
D
Weinberg
Christin
C
Fuchs
Stephan
S
Schmudde
Mareike
M
Bröker
Barbara M
BM
Wolz
Christiane
C
Hecker
Michael
M
Engelmann
Susanne
S
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D000071677:Histidine Kinase; D009154:Mutation; D011494:Protein Kinases / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics*; D014157:Transcription Factors; D014158:Transcription, Genetic; D037521:Virulence Factors / Q000378:metabolism
D001426:Bacterial Proteins; C433805:SaeR protein, Staphylococcus aureus; D014157:Transcription Factors; D037521:Virulence Factors; D011494:Protein Kinases; D000071677:Histidine Kinase