title: Optimization of an ethanol production medium in very high gravity fermentation.
abstract: Concentrations of Mg(2+), glycine, yeast extract, biotin, acetaldehyde and peptone were optimized by a uniform design process for ethanol production by Saccharomyces cerevisiae. Using non-linear step-wise regression analysis, a predictive mathematical model was established. Concentrations of Mg(2+) and peptone were identified as the critical factors: 50 mM Mg(2+) and 1.5% (w/v) peptone in the medium increased the final ethanol titre from 14.2% (v/v) to 17% (v/v) in 48 h.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China.
authors.forename: Fan-Qiang
authors.initials: FQ
authors.lastname: Wang
authors.forename: Cui-Juan
authors.initials: CJ
authors.lastname: Gao
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000079:Acetaldehyde / Q000494:pharmacology; D001710:Biotin / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005998:Glycine / Q000494:pharmacology; D018471:Hypergravity; D007218:Industrial Microbiology / Q000379:methods*; D008962:Models, Theoretical; D010461:Peptones / Q000494:pharmacology; D012044:Regression Analysis; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism
chemical_list: D003470:Culture Media; D010461:Peptones; D000431:Ethanol; D001710:Biotin; D000079:Acetaldehyde; D005998:Glycine