Evidence for the presence of lipofibroblasts in human lung.
The lipid-containing alveolar interstitial fibroblast (lipofibroblast) is known to be critically involved in rodent lung development, homeostasis, and injury/repair. However, there is lack of information on their presence and function in the human lung. Based on a number of morphological (lipid staining), molecular (presence of characteristic lipogenic and absence of myogenic markers), and functional (triglyceride uptake) characteristics that are the hallmarks of the rodent lung lipofibroblast, using human lung fibroblasts of embryonic (WI-38) and adult origin and lung tissue from human autopsy specimens, the authors for the first time clearly demonstrate the presence of lipofibroblasts in the human lung.
Rehan
Virender K
VK
Department of Pediatrics, Los Angeles Biomedical Research Institute at Harbor-UCLA, David Geffen School of Medicine, University of California, Los Angeles, California 90502, USA. vrehan@labiomed.org
Sugano
Sharon
S
Wang
Ying
Y
Santos
Jamie
J
Romero
Sonia
S
Dasgupta
Chiranjib
C
Keane
Michael P
MP
Stahlman
Mildred T
MT
Torday
John S
JS
D000199:Actins / Q000235:genetics; D000328:Adult; D001391:Azo Compounds; D015415:Biomarkers; D002478:Cells, Cultured; D004396:Coloring Agents; D005333:Fetus / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics; D044162:Parathyroid Hormone-Related Protein / Q000235:genetics; D011650:Pulmonary Alveoli / Q000166:cytology*; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014280:Triglycerides / Q000493:pharmacokinetics*
D000199:Actins; D001391:Azo Compounds; D015415:Biomarkers; D004396:Coloring Agents; D047495:PPAR gamma; D044162:Parathyroid Hormone-Related Protein; D012333:RNA, Messenger; D014280:Triglycerides; C011049:oil red O