Production of penicillin acylase by a recombinant Escherichia coli using cheese whey as substrate and inducer.
Cheese whey (CW) is the major subproduct from cheese manufacturing and it is considered as a waste pollutant since its high content of lactose. In this work a fermentation process for the production of penicillin acylase (PA) by a recombinant Escherichia coli and using CW as unique carbon source and inducer was developed. A design factorial 3(2) was used to evaluate the influence of independent variables (dissolved oxygen and CW concentration) on the ability of E. coli W3110/pPA102 to produce PA. Maximum specific PA activity of 781 U g(-1) was attained at 5 g L(-1) of CW and 3% dissolved oxygen. The results showed that CW can be used successfully as unique carbon source and inducer for the production of recombinant proteins using constructions driven by the lac promoter and this way reducing the discharges of that pollutant to the environment.
De León-Rodríguez
Antonio
A
División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Apartado Postal 3-74 Tangamanga, 78231 San Luis Potosí, S.L.P., Mexico. aleonr@ipicyt.edu.mx
Rivera-Pastrana
Dulce
D
Medina-Rivero
Emilio
E
Flores-Flores
José Luis
JL
Estrada-Baltazar
Alejandro
A
Ordóñez-Acevedo
Leandro G
LG
de la Rosa
Ana P Barba
AP
D002611:Cheese; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D007763:Lac Operon; D007785:Lactose / Q000378:metabolism; D010399:Penicillin Amidase / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
D003470:Culture Media; D011994:Recombinant Proteins; D010399:Penicillin Amidase; D007785:Lactose