Prolactin-induced changes in protein expression in human pancreatic islets.
Ex vivo islet cell culture prior to transplantation appears as an attractive alternative for treatment of type 1 diabetes. Previous results from our laboratory have demonstrated beneficial effects of human prolactin (rhPRL) treatment on human islet primary cultures. In order to probe into the molecular events involved in the intracellular action of rhPRL in these cells, we set out to identify proteins with altered expression levels upon rhPRL cell treatment, using two-dimensional (2D) gel electrophoresis and mass spectrometry (MS). An average of 300 different protein spots were detected, 14 of which were modified upon rhPRL treatment (p<0.01), of which 12 were successfully identified using MS and grouped according to their biological functions. In conclusion, our study provides, for the first time, information about proteins that could be critically involved in PRL's action on human pancreatic islets, and facilitate identification of new and specific targets involved in islet cell function and proliferation.
Labriola
L
L
Department of Biochemistry, Chemistry Institute, University of Sao Paulo, Av. Prof. Lineu Prestes, 748, Bloco 9 Superior Sala 964, São Paulo 05508-900 SP, Brazil.
Ferreira
G Bomfim
GB
Montor
W R
WR
Demasi
M A A
MA
Pimenta
D C
DC
Lojudice
F H
FH
Genzini
T
T
Goldberg
A C
AC
Eliaschewitz
F G
FG
Sogayar
M C
MC
D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D011388:Prolactin / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D046509:Tissue Culture Techniques
D011993:Recombinant Fusion Proteins; D011388:Prolactin