[Analysis of POU1F1 gene polymorphisms in Qinchuan cattle and Chinese Holstein cattle].
PCR-RFLP was applied to analyze the polymorphisms of POU1F1 gene in 218 Qinchuan cattle (QQ) and Chinese Holstein cattle (HC). Results demonstrated Hinf I polymorphisms in the 451 bp PCR product in the two populations. The frequencies of alleles A/B in QQ and HC populations were 0.232/0.768 and 0.132/0.868, respectively. The frequencies of three genotypes AA, AB and BB were 0.030/0.403/0.567 and 0.007/0.251/0.742, respectively. Qinchuan cattle population was at Hardy-Weinberg equilibrium at this locus, but Chinese Holstein cattle population was not. The gene heterozygosity/effective allele gene number/Shannon information entropy/polymorphism information content of Qinchuan cattle and Chinese Holstein cattle populations were listed for 0.356/1.553/0.541/0.292 and 0.229/1.297/0.390/0.203, respectively. All indices were higher in the Qinchuan cattle population.
Yan
Lin-Jun
LJ
Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China. ylj_23@sohu.com
Liu
Bo
B
Fang
Xin-Tang
XT
Chen
Hong
H
Zhang
Run-Feng
RF
Bao
Bin
B
Zhang
Hai-Jun
HJ
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002681:China; D005091:Exons / Q000235:genetics; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D007438:Introns / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D050817:Transcription Factor Pit-1 / Q000235:genetics*
D050817:Transcription Factor Pit-1