title: Antinociceptive and anti-inflammatory activities of Dicranopteris linearis leaves chloroform extract in experimental animals.
abstract: The present study was carried out to establish the antinociceptive and anti-inflammatory properties of Dicranopteris linearis leaves chloroform extract in experimental animals. The antinociceptive activity was measured using the abdominal constriction, formalin and hot plate tests, while the anti-inflammatory activity was measured using the carrageenan-induced paw edema. The extract, obtained after 72 h soaking of the air-dried leaves in chloroform followed by evaporation under vacuo (40 degrees C) to dryness, was dissolved in dimethyl sulfoxide to the doses of 20, 100 and 200 mg/kg and administered subcutaneously 30 min prior to subjection to the above mentioned assays. The extract, at all doses used, was found to exhibit significant (p<0.05) antinociceptive activity in a dose-dependent manner. However, the significant (p<0.05) anti-inflammatory activity observed occur in a dose-independent manner. As a conclusion, the chloroform extract of D. linearis possesses antinociceptive and anti-inflammatory activity and thus justify its traditional uses by the Malays to treat various ailments.
authors.affiliation: Faculty of Biotechnology and Life Sciences, Universiti Industri Selangor, Jalan Zirkon A7/A, Seksyen 7, 40000 Shah Alam, Selangor, Malaysia. shaza8174@yahoo.com
authors.forename: Zainul Amiruddin
authors.initials: ZA
authors.lastname: Zakaria
authors.forename: Zuleen Delina Fasya
authors.initials: ZD
authors.lastname: Abdul Ghani
authors.forename: Raden Nur Suraya
authors.initials: RN
authors.lastname: Raden Mohd Nor
authors.forename: Hanan Kumar
authors.initials: HK
authors.lastname: Gopalan
authors.forename: Mohd Roslan
authors.initials: MR
authors.lastname: Sulaiman
authors.forename: Fatimah Corazon
authors.initials: FC
authors.lastname: Abdullah
mesh_terms: D000818:Animals; D002725:Chloroform; D004121:Dimethyl Sulfoxide; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000188:drug therapy*; D005334:Fever / Q000188:drug therapy*; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010146:Pain / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012996:Solutions
chemical_list: D010936:Plant Extracts; D012996:Solutions; D002725:Chloroform; D004121:Dimethyl Sulfoxide