Penile, urethral, and seminal sampling for diagnosis of human papillomavirus infection in men.
Methods that used specimens from three genital sites (penile brushing [PB], urethral brushing [UB], and the retrieval of semen [SE]) from 50 men were examined for human papillomavirus (HPV) DNA detection. The rates of detection by PB, UB, SE, PB and UB, and PB and SE were 88.9%, 50.0%, 33.3%, 100%, and 97.2%, respectively. The use of PB and UB appears to be the most accurate method; as an alternative to UB, the use of SE with PB could be used to improve the rate of HPV DNA detection in men.
Giovannelli
Lucia
L
Dipartimento di Igiene e Microbiologia, Università di Palermo, Palermo, Italy. lucia.giov@unipa.it
Migliore
Maria Colomba
MC
Capra
Giuseppina
G
Caleca
Maria Pia
MP
Bellavia
Carmelina
C
Perino
Antonio
A
Viviano
Enza
E
Matranga
Domenica
D
Ammatuna
Pietro
P
D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D008297:Male; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D010413:Penis / Q000821:virology*; D016133:Polymerase Chain Reaction; D012661:Semen / Q000821:virology*; D013048:Specimen Handling / Q000379:methods*; D014521:Urethra / Q000821:virology*; D014860:Warts / Q000821:virology
D004279:DNA, Viral