[Transfer of a rye small chromosomal segment with powdery mildew-resistant gene(s) into common wheat (Triticum aestivum L.)].
The advanced progeny lines (BC1F5) from the monosomic addition lines between common wheat cultivar Mianyang 11, which is highly susceptible to powdery mildew, and an inbred rye line R12 were analyzed for selection of wheat-rye translocations. Based on a rye-specific repetitive sequence of pSc20H, which spread over all chromosomes of rye but did not existed in wheat, a set of PCR primer was designed and used to identify the rye chromosome segments in wheat. From 300 of the BC1F5 progeny lines 70 were found to contain chromosome composition of rye. An advanced line, 96II691-830-98, originated from 6R monosomic addition line was observed to be immune to powdery mildew, different from its wheat parent Mianyang 11. A small segment of rye chromosome at telomere in a pair of wheat chromosome in the line was found by means of GISH. The results indicated that a small segment of rye chromosome 6R carrying the gene(s) for resistance to powdery mildew has been transferred into common wheat. In the progeny of monosomic addition lines a high frequency of wheat-alien species translocation with various segments of chromosomes could be found by application of both PCR and GISH technique.
Fu
Shu-Lan
SL
State Key Laboratory for Plant Genetics and Breeding, Sichuan Agricultural University, Ya'an 625014, China. shulanfu@sohu.com
Tang
Zong-Xiang
ZX
Zhang
Huai-Qiong
HQ
Yang
Zu-Jun
ZJ
Ren
Zheng-Long
ZL
D002843:Chromatin / Q000378:metabolism; D032461:Chromosomes, Plant / Q000235:genetics*; D018014:Gene Transfer Techniques; D007113:Immunity, Innate / Q000235:genetics*; D017403:In Situ Hybridization; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012434:Secale / Q000166:cytology / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D014908:Triticum / Q000235:genetics*
D002843:Chromatin