title: NMR investigation of DNA primer-template models: structural insights into dislocation mutagenesis in DNA replication.
abstract: Slipped frameshift intermediates can occur when DNA polymerase slows or stalls at sites of DNA lesions. However, this phenomenon is much less common when unmodified DNA is replicated. In order to study the effect of templating bases on the alignment of primer-templates, NMR structural investigation has been performed on primer-template oligonucleotide models which mimic the situation that dNTP has just been incorporated opposite template. NMR evidence reveals the occurrence of misalignment when dGTP is incorporated opposite template T with a downstream nucleotide C. Depending on the template sequence, further extension of the primer can lead to realignment.
authors.affiliation: Department of Chemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
authors.forename: Lai Man
authors.initials: LM
authors.lastname: Chi
authors.forename: Sik Lok
authors.initials: SL
authors.lastname: Lam
mesh_terms: D020137:Base Pair Mismatch; D020029:Base Pairing; D017931:DNA Primers / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics*; D016368:Frameshift Mutation; D008957:Models, Genetic; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods
chemical_list: D017931:DNA Primers