title: Cell adhesion and migration assays.
abstract: Adhesion and migration are basic responses of living cells to environmental stimuli. Such responses are central to a broad range of physiological processes, such as the immune response, repair of injured tissues, and prevention of excessive bleeding. Cell adhesion and migration also contributes to pathologies, including vascular and inflammatory diseases, as well as tumor growth and metastasis. These cellular responses depend on engagement of adhesion receptors by components of the extracellular matrix or molecules present on the surface of other cells. Hence, cell adhesion and migration assays are crucial methods in cell biology. In this chapter, several detailed protocols describing cell adhesion and migration assays are presented, and advantages and disadvantages of each method are discussed.
authors.affiliation: Joseph J. Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, The Cleveland Clinic Foundation, OH, USA.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Soloviev
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Pluskota
authors.forename: Edward F
authors.initials: EF
authors.lastname: Plow
mesh_terms: D002318:Cardiovascular Diseases / Q000503:physiopathology*; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D003584:Cytological Techniques / Q000379:methods*; D006801:Humans