title: Lipopolysaccharide-stimulated responses in rat aortic endothelial cells by a systems biology approach.
abstract: The vascular endothelium plays an important role in regulating immune and inflammatory responses to resist pathogens infection. Although it has been known that lipopolysaccharide (LPS) is a critical inducer of sepsis or endotoxemia, the systematic responses of LPS-stimulation in endothelial cells (ECs) are still unclear. The present study aims to analyze the late-phase responses of LPS-induced rat aortic ECs by using systematic biology approaches, including rat cDNA microarray, 2-DE and MALDI-TOF MS/MS, and cytokine protein array. Furthermore, to improve the efficiency of analysis of the bulk systematic data of rat, we designed a set of bioinformatic tools to convert and integrate these rat data into the corresponding human genes or proteins IDs based on BioCarta, KEGG, and Gene Ontology databases. Using the systematic analysis, it was shown that LPS could promote some signaling or metabolic pathways as well as pathophysiologic phenomena of proliferation, atherogenesis, inflammation, and apoptosis through activated nuclear factor-kappaB pathway in ECs. Interestingly, ECs also activated the mediators of anti-inflammation, antiapoptosis, and antioxidation to protect themselves. Moreover, the expressions of altered genes, proteins, and their involvement in the hypothetical signaling pathway can provide further understanding of inflammation associated responses in ECs.
authors.affiliation: Department and Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Hsiang-Wen
authors.initials: HW
authors.lastname: Tseng
authors.forename: Hsueh-Fen
authors.initials: HF
authors.lastname: Juan
authors.forename: Hsuan-Cheng
authors.initials: HC
authors.lastname: Huang
authors.forename: John Yi-Chung
authors.initials: JY
authors.lastname: Lin
authors.forename: Supachok
authors.initials: S
authors.lastname: Sinchaikul
authors.forename: Tzi-Chung
authors.initials: TC
authors.lastname: Lai
authors.forename: Chieh-Fu
authors.initials: CF
authors.lastname: Chen
authors.forename: Shui-Tein
authors.initials: ST
authors.lastname: Chen
authors.forename: Guei-Jane
authors.initials: GJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects*; D002478:Cells, Cultured; D016000:Cluster Analysis; D016207:Cytokines / Q000494:pharmacology; D030541:Databases, Genetic; D004730:Endothelium, Vascular / Q000187:drug effects*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008954:Models, Biological; D018448:Models, Immunological; D016328:NF-kappa B / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D049490:Systems Biology
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B