Ovarian tumors in Rbp9 mutants of Drosophila induce an immune response.
The Drosophila protein, Rbp9, is homologous to human Hu, which is reported to be involved in small cell lung cancer. Rbp9 functions in cystocyte differentiation, and mutations in Rbp9 cause ovarian tumors. Here we show that the antimicrobial peptide, Attacin, is upregulated in Rbp9 mutants, especially in ovaries where tumors form. Upregulation seems to result from activation of the NF-kappaB pathway since we detected nuclear localization of Relish in Rbp9 mutant ovaries but not in wild type ovaries. Inactivation of NF-kappaB in the Rbp9 mutant allows prolonged survival of malformed egg chambers. We conclude that Drosophila initiates an anti-tumor defense response via activation of NF-kappaB.
Kim
Jihyun
J
Department of Molecular Biology, School of Natural Sciences, Sejong University, Seoul 143-747, Korea.
Kim-Ha
Jeongsil
J
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D004195:Disease Models, Animal; D004330:Drosophila / Q000276:immunology*; D029721:Drosophila Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D005260:Female; D020869:Gene Expression Profiling; D008297:Male; D009154:Mutation; D016328:NF-kappa B / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000502:physiology; D010051:Ovarian Neoplasms / Q000235:genetics / Q000276:immunology; D010053:Ovary / Q000276:immunology / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D014157:Transcription Factors / Q000032:analysis; D015854:Up-Regulation
C488989:AttC protein, Drosophila; D029721:Drosophila Proteins; D016328:NF-kappa B; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; C079911:Rbp9 protein, Drosophila; C101626:Rel protein, Drosophila; D014157:Transcription Factors