Sorbitol regulates energy transfer from allophycocyanin to the terminal emitter within phycobilisomes in Synechocystis sp.
The effects of sorbitol on energy transfer of phycobilisomes (PBSs) in vivo were investigated in a chlN deletion mutant of Synechocystis sp. PCC 6803. When the mutant was grown in the dark, it contained intact and functional PBSs but essentially no chlorophyll or photosystems. Therefore, the structural and functional changes of the mutant PBSs in vivo can be detected by measurement of low temperature (77 K) and room temperature fluorescence emission spectra. Our results, for the first time, demonstrate that sorbitol decreases the energy transfer from allophycocyanin to the terminal emitter, indicating the site for osmotic regulation of excitation transfer in PBSs.
Liu
Xingguo
X
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
Zhao
Jingjing
J
Wu
Qingyu
Q
D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism; D004735:Energy Transfer / Q000187:drug effects; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics; D009997:Osmotic Pressure; D045524:Phycobilisomes / Q000737:chemistry / Q000378:metabolism*; D010798:Phycocyanin / Q000737:chemistry / Q000378:metabolism*; D013012:Sorbitol / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D046939:Synechocystis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D045524:Phycobilisomes; C002497:allophycocyanin; D010798:Phycocyanin; D002734:Chlorophyll; D013012:Sorbitol