Electrochemical detection of short sequences related to the hepatitis B virus using MB on chitosan-modified CPE.
A novel electrochemical DNA biosensor based on methylene blue (MB) and chitosan-modified carbon paste electrode (CCPE) for short DNA sequences and polymerase chain reaction (PCR) amplified real samples related to the hepatitis B virus (HBV) hybridization detection is presented. Differential pulse voltammetry (DPV) was used to investigate the surface coverage and hybridization event. The decrease in the peak current of MB, an electroactive label, was observed upon hybridization of probe with the target. Numerous factors affecting the target hybridization and indicator binding reaction are optimized to maximize the sensitivity.
Mandong
Guo
G
Department of Chemistry, Shanxi Normal University, Linfen 041004, Shanxi, China. guomd@dns.sxtu.edu.cn <guomd@dns.sxtu.edu.cn>
Yanqing
Li
L
Hongxia
Guo
G
Xiaoqin
Wu
W
Lifang
Fan
F
D001483:Base Sequence; D002244:Carbon / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification*; D017403:In Situ Hybridization / Q000295:instrumentation* / Q000379:methods; D008751:Methylene Blue / Q000737:chemistry*; D008839:Microelectrodes; D008969:Molecular Sequence Data; D009824:Ointments / Q000737:chemistry; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry / Q000235:genetics
D020099:Coated Materials, Biocompatible; D004279:DNA, Viral; D009824:Ointments; D009841:Oligonucleotides; D002244:Carbon; D048271:Chitosan; D008751:Methylene Blue