Telemetric recording of intrapleural pressure.
BACKGROUND
Monitoring of intrapleural pressure (IPP) is used for evaluation of lung function in a number of pathophysiological conditions. We describe a telemetric method of non-invasive monitoring of the IPP in conscious animals intermittently or continuously for a prolonged period of time.


METHODS
After IACUC approval, six mongrel dogs were used for the study. After sedation, each dog was intubated and anesthetized using 0.5% Isoflurane. A telemetric implant model TL11M2-D70-PCT from Data Science International was secured subcutaneously. The pressure sensor tip of the catheter from the implant was inserted into the pleural space, and the catheter was secured with sutures. The IPP signals were recorded at a sampling rate of 100 points/second for 30 to 60 min daily for 4 days. From these recordings, the total mean negative IPP (mmHg), and the total mean negative IPP for a standard time of 30 min were calculated. In addition, the actual inspiratory and expiratory pressures were also measured from stable recording of the IPP waveforms.


RESULTS
In six dogs, the total mean +/- SD negative IPP was -10.8 +/- 10.6 mmHg. After normalizing with respect to acquisition time it was -13.2 +/- 11.2 mmHg/min. The actual inspiratory pressure was -19.7 +/- 15.3, and the expiratory pressure was -11.0 +/- 12.9.


CONCLUSIONS
Our study demonstrates that telemetric monitoring of IPP can be performed reliably and non-invasively in conscious experimental animals. The values for IPP in our study are compatible with the results of other investigators who used different methods of IPP measurement. Further work may show this method to be helpful in understanding the pathophysiology of various breathing disorders.
Ednick
Mathew D
MD
Department of Pediatrics, Maimonides Infants and Children's Hospital, Brooklyn, New York 11219, USA.
Pagala
Murali
M
Barakat
John-Pierre
JP
Nino
Gustavol
G
Shah
Prashant
P
Cunningham
Joseph N
JN
Vaynblat
Mikhail
M
Kazachkov
Mikhail
M
D000818:Animals; D002404:Catheterization; D003243:Consciousness; D004285:Dogs; D045853:Exhalation / Q000502:physiology*; D001239:Inhalation / Q000502:physiology*; D008365:Manometry / Q000295:instrumentation* / Q000379:methods; D023421:Models, Animal; D009043:Motor Activity; D035422:Pleural Cavity / Q000502:physiology*; D011312:Pressure; D013686:Telemetry / Q000295:instrumentation* / Q000379:methods