Human recombinant PLD2 can repress p65 activity of guinea pigs of chronic asthma in vivo.
This article is to investigate the effect of human recombinant phospholipase D2 (rhPLD2) in vivo on the expression of nuclear transcription factor p65 in chronic asthma of guinea pigs. After treating the guinea pigs with chronic asthma by rhPLD2, the crude nuclear extraction was assayed with TransAM Transcription Factor Assay Kit for the activity of pulmo tissue nuclear transcription factor p65. Compared with the healthy guinea pigs, the activity of nuclear transcription factor p65 in guinea pigs of chronic asthma is much higher than that of control groups. Our results showed that rhPLD2 markedly depressed the activity of p65 when the guinea pigs were attacked by chronic asthma.
Zhu
Ling
L
Immunology Department and Center of Neuroscience, Fujian Medical University, Fujian, China. zhling62@pub6.fz.fj.cn
Zou
Weibin
W
Yu
Chuanxing
C
Lin
Junjin
J
He
Xiaoli
X
D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology; D001249:Asthma / Q000188:drug therapy* / Q000503:physiopathology*; D002908:Chronic Disease; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006801:Humans; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010739:Phospholipase D / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D051996:Transcription Factor RelA / Q000378:metabolism*
D018927:Anti-Asthmatic Agents; D011994:Recombinant Proteins; D051996:Transcription Factor RelA; C105606:phospholipase D2; D010739:Phospholipase D