title: Bionanoconjugation via click chemistry: The creation of functional hybrids of lipases and gold nanoparticles.
abstract: A simple and versatile method for the preparation of functional enzyme-gold nanoparticle conjugates using "click" chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalized Thermomyces lanuginosus lipase has been attached to azide-functionalized water-soluble gold nanoparticles under retention of enzymatic activity. The products have been characterized by gel electrophoresis and a fluorometric lipase activity assay. It is estimated that the equivalent of approximately seven fully active lipase molecules are attached to each nanoparticle.
authors.affiliation: Centre for Nanoscale Science, Department of Chemistry, University of Liverpool, Liverpool, L69 7ZD, U.K.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Brennan
authors.forename: Nikos S
authors.initials: NS
authors.lastname: Hatzakis
authors.forename: T Robert
authors.initials: TR
authors.lastname: Tshikhudo
authors.forename: Nijole
authors.initials: N
authors.lastname: Dirvianskyte
authors.forename: Valdemaras
authors.initials: V
authors.lastname: Razumas
authors.forename: Shamkant
authors.initials: S
authors.lastname: Patkar
authors.forename: Jesper
authors.initials: J
authors.lastname: Vind
authors.forename: Allan
authors.initials: A
authors.lastname: Svendsen
authors.forename: Roeland J M
authors.initials: RJ
authors.lastname: Nolte
authors.forename: Alan E
authors.initials: AE
authors.lastname: Rowan
authors.forename: Mathias
authors.initials: M
authors.lastname: Brust
mesh_terms: D006046:Gold / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D006046:Gold; D008049:Lipase