An integrated map of p53-binding sites and histone modification in the human ENCODE regions.
TP53 (tumor protein p53; p53) regulates its target genes under various cellular stresses. By combining chromatin immunoprecipitation with oligonucleotide microarrays, we have mapped binding sites of p53 (p53-BS) in the genome of HCT116 human colon carcinoma cells, along with those of acetylated H3, acetylated H4, and methylated H3-K4. We analyzed a 30-Mb portion of the human genome selected as a representative model by the ENCODE Consortium. In the region, we found 37 p53-BS, of which the p53-binding motif was present in 32 (86%). Acetylated histone H3 and H4 were detected at 14 (38%) and 33 (89%) of the p53-BS, respectively. A significant portion (58%) of H4 acetylation in the p53-BS was not accompanied by H3 acetylation. Acetyl H3 were preferentially located at the 5' and 3' ends of genes, whereas acetyl H4 were distributed widely across the genome. These results provide novel insights into how p53 binding coordinates with histone modification in human.
Kaneshiro
Kiyofumi
K
Genome Science Division, Research Center for Advanced Science and Technology (RCAST), University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8904, Japan.
Tsutsumi
Shuichi
S
Tsuji
Shingo
S
Shirahige
Katsuhiko
K
Aburatani
Hiroyuki
H
D000107:Acetylation; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics / Q000378:metabolism; D016158:Genes, p53; D015894:Genome, Human; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D015533:Transcriptional Activation; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D006657:Histones; D016159:Tumor Suppressor Protein p53; D004247:DNA