Orientation and penetration depth of monolayer-bound p40phox-PX.
X-ray reflectivity was used to study the interaction of the PX domain of p40(phox) protein (p40(phox)-PX) with a Langmuir monolayer of a mixture of SOPC (1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine), SOPS (1-stearoyl-2-oleoyl-sn-glycero-3-phosphoserine), and DPPtdIns(3)P (1,2-dipalmitoylphosphatidylinositol 3-phosphate) lipids supported on a buffered aqueous solution. The reflectivity is analyzed in terms of the known crystallographic structure of the p40(phox)-PX domain and a slab model that represents the lipid layer, yielding an electron density profile of the lipid layer and bound PX domains. This analysis determines the angular orientation and penetration depth of the p40(phox)-PX domain bound to the SOPC/SOPS/DPPtdIns(3)P monolayer. The best fit orientation is characterized by the following angles: theta = 30 +/- 10 degrees and phi = 140 +/- 30 degrees. These angles describe rotations, about axes in a coordinate system fixed to the domain, that are required to orient the domain with respect to the lipid layer at the interface. The protein penetrated into the lipid layer by 9 +/- 2 A, indicating that the protein penetrated into the headgroup region, but not deeply into the hydrocarbon region of the monolayer. In this analysis, polar Tyr(94) and hydrophobic Val(95) penetrated deepest into the lipid monolayer. The backbone of these residues was approximately 5 A above the headgroup-buffer interface, i.e., at the level of the SOPC/SOPS lipid phosphates. Positively charged Lys(92) and Lys(98) were also near the SOPC/SOPS lipid phosphates. This position of the protein allows for a favorable electrostatic contribution to binding.
Málková
Sárka
S
Department of Physics, University of Illinois, Chicago, Illinois 60607-7059, USA.
Stahelin
Robert V
RV
Pingali
Sai V
SV
Cho
Wonhwa
W
Schlossman
Mark L
ML
D008567:Membranes, Artificial; D019255:NADPH Oxidases / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D018129:Phosphatidylinositol Phosphates / Q000737:chemistry; D010718:Phosphatidylserines / Q000737:chemistry; D014961:X-Ray Diffraction
D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D018129:Phosphatidylinositol Phosphates; D010718:Phosphatidylserines; C027891:1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1