[Effects of sodium arsenite on catalase in human keratinocytes].
OBJECTIVE
To evaluate the effects of sodium arsenite on the activity, the mRNA and the protein expression of CAT in human keratinocyte cell line (HaCaT).


METHODS
The activity of catalase (CAT) was detected by ultraviolet direct velocity assay. RT-PCR was used to detect the mRNA expression of CAT and Western blotting was conducted to detect the protein expression of CAT.


RESULTS
If the cells were treated with higher than 5.0 micromol/L sodium arsenite, the activity, mRNA and protein expression of CAT were decreased significantly and in a dosage dependent fashion (P < 0.05).


CONCLUSIONS
CAT is inhibited by sodium arsenite in the transcription, translation and activity levels.
Sun
Xian-ce
XC
Hygiene Department, Colleges of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116027, China.
Piao
Feng-yuan
FY
Wang
Yi
Y
Xu
Yuan-yuan
YY
Li
Xin
X
Li
Bing
B
Jin
Ya-ping
YP
Sun
Gui-fan
GF
D018053:Arsenites / Q000633:toxicity*; D015153:Blotting, Western; D002374:Catalase / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017670:Sodium Compounds / Q000633:toxicity*
D018053:Arsenites; D012333:RNA, Messenger; D017670:Sodium Compounds; C017947:sodium arsenite; D002374:Catalase