title: Targeted chromosome elimination from ES-somatic hybrid cells.
abstract: To engineer a stem cell genome, we developed a technology for targeted elimination of chromosomes from mouse embryonic stem (ES)-somatic hybrid cells. Here we demonstrate the use of a universal chromosome elimination cassette (CEC) for elimination of a single embryonic stem cell (ESC)-derived chromosome 11 or 12, and also both copies of chromosome 6, which harbor pluripotency-associated genes including Nanog. We attribute hybrid-cell pluripotency to the expression of Nanog from the reprogrammed somatic-cell nuclei.
authors.affiliation: Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Matsumura
authors.forename: Masako
authors.initials: M
authors.lastname: Tada
authors.forename: Tomomi
authors.initials: T
authors.lastname: Otsuji
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yasuchika
authors.forename: Norio
authors.initials: N
authors.lastname: Nakatsuji
authors.forename: Azim
authors.initials: A
authors.lastname: Surani
authors.forename: Takashi
authors.initials: T
authors.lastname: Tada
mesh_terms: D000818:Animals; D002872:Chromosome Deletion; D002875:Chromosomes / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D005821:Genetic Techniques; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D006822:Hybrid Cells; D051379:Mice; D000071317:Nanog Homeobox Protein; D013234:Stem Cells
chemical_list: D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse