Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization.
The entire gene cluster involved in the biosynthesis of angucyclines Sch 47554 and Sch 47555 was cloned, sequenced, and characterized. Analysis of the nucleotide sequence of genomic DNA spanning 77.5-kb revealed a total of 55 open reading frames, and the deduced products exhibited strong sequence similarities to type II polyketide synthases, deoxysugar biosynthetic enzymes, and a variety of accessory enzymes. The involvement of this gene cluster in the pathway of Sch 47554 and Sch 47555 was confirmed by genetic inactivation of the aromatase, including a portion of the ketoreductase, which was disrupted by inserting the thiostrepton gene.
Basnet
Devi Bahdur
DB
Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University, Asan 336-708, Korea.
Oh
Tae-Jin
TJ
Vu
Thi Thu Hang
TT
Sthapit
Basundhara
B
Liou
Kwangkyoung
K
Lee
Hei Chan
HC
Yoo
Jin-Cheol
JC
Sohng
Jae Kyung
JK
D001483:Base Sequence; D001551:Benz(a)Anthracenes / Q000737:chemistry / Q000378:metabolism; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D048630:Polyketide Synthases / Q000235:genetics; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
D001551:Benz(a)Anthracenes; C081748:Sch 47554; C081749:Sch 47555; D048630:Polyketide Synthases