title: Cadmium response and redoxin targets in Chlamydomonas reinhardtii: a proteomic approach.
abstract: A proteomic approach including two-dimensional electrophoresis and MALDI-TOF analysis has been developed to identify the soluble proteins of the unicellular photosynthetic algae Chlamydomonas reinhardtii. We first described the partial 2D-picture of soluble proteome obtained from whole cells grown on acetate. Then we studied the effects of the exposure of these cells to 150 muM cadmium (Cd). The most drastic effect was the decrease in abundance of both large and small subunits of the ribulose-1,5-bisphosphate carboxylase/oxygenase, in correlation with several other enzymes involved in photosynthesis, Calvin cycle and chlorophyll biosynthesis. Other down-regulated processes were fatty acid biosynthesis, aminoacid and protein biosynthesis. On the other hand, proteins involved in glutathione synthesis, ATP metabolism, response to oxidative stress and protein folding were up-regulated in the presence of cadmium. In addition, we observed that most of the cadmium-sensitive proteins were also regulated via two major cellular thiol redox systems, thioredoxin and glutaredoxin.
authors.affiliation: IBBMC, CNRS UMR 8619, Bat 430, Univ Paris-Sud, Orsay cedex, 91405, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Gillet
authors.forename: Paulette
authors.initials: P
authors.lastname: Decottignies
authors.forename: Solenne
authors.initials: S
authors.lastname: Chardonnet
authors.forename: Pierre
authors.initials: P
authors.lastname: Le Maréchal
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D050260:Carbohydrate Metabolism; D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000378:metabolism*; D002734:Chlorophyll / Q000096:biosynthesis; D005227:Fatty Acids / Q000096:biosynthesis; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D005943:Gluconeogenesis / Q000502:physiology; D005978:Glutathione / Q000378:metabolism; D006038:Glyoxylates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D013213:Starch / Q000096:biosynthesis; D013455:Sulfur / Q000378:metabolism; D013879:Thioredoxins / Q000378:metabolism
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D006038:Glyoxylates; D010940:Plant Proteins; D020543:Proteome; D002104:Cadmium; D002734:Chlorophyll; D013879:Thioredoxins; D013455:Sulfur; D013213:Starch; D005978:Glutathione; C031150:glyoxylic acid; D009584:Nitrogen