[The relationship between endothelin-1 and tumor necrosis factor-alpha and myocardial microcirculation dysfunction].
OBJECTIVE
To investigate the relationship between endothelin-1 (ET-1) and tumor necrosis factor-alpha and myocardial microcircular dysfunction during coronary microembolization (CME).


METHODS
CME was induced in 10 miniswine by selective infusion of microspheres (45 microm) into left anterior descending artery (LAD). We measured (1) coronary sinus level of ET-1, TNF-alpha using radioimmunoassay; (2) CFR, a measure of microvascular integrity, using Doppler flow wire in LAD at baseline and different doses of microspheres.


RESULTS
CFR decrease significantly with different doses of microspheres (vs. baseline, P < 0.05). Level of ET-1, TNF-alpha increased significantly with doses of 5 x 10(4) and peaked with 10 x 10(4). Interestingly, ET-1 progressively decrease while TNF-alpha persistently elevated from doses of 12 x 10(4) to 15 x 10(4). There are reverse correlations between ET-1 and CFR (r = -0.31, P < 0.05).


CONCLUSIONS
The extent of microvascular injury wasn't linearly related to the extent of ME, where, it closely associated with myocardial ET-1.
Zhang
Qing-yong
QY
Department of Cardiology, the NO.6 People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China. yeakcardio@yahoo.com.cn
Ge
Jun-bo
JB
Zhu
Jian-hua
JH
Chen
Jun-zhu
JZ
Zhang
Liang-hui
LH
Qian
Ju-ying
JY
D000818:Animals; D003328:Coronary Thrombosis / Q000503:physiopathology*; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000097:blood*; D008833:Microcirculation / Q000503:physiopathology*; D013552:Swine; D013556:Swine, Miniature; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
D019332:Endothelin-1; D014409:Tumor Necrosis Factor-alpha