Alpha(0)-thalassemia and related disorders in northeast Thailand: a molecular and hematological characterization.
Alpha(0)-thalassemia is the most severe form of alpha-thalassemia commonly encountered in Asians. To provide relevant information for effective prevention and control of this disorder, we have examined the molecular basis and hematological features of alpha(0)-thalassemia-related disorders in northeast Thailand. A multiplex polymerase chain reaction for simultaneous detection of the southeast Asian (SEA) and the THAI alpha(0)-thalassemia determinants was developed and used for screening of 1,541 Thai individuals who were positive at the preliminary screening in an ongoing thalassemia control program. Alpha(0)-thalassemia deletions were detected in 397 (25.8%) cases, 396 with the SEA deletion and 1 with the THAI deletion. While the latter was found in association with the Hb Constant Spring in a patient with severe Hb H disease, the former was encountered in as many as 12 thalassemia genotypes whose hematological features were comparatively presented. The results obtained should prove useful in carrier screening and prenatal diagnosis programs of this common genetic disorder in the region.
Sae-ung
Nattaya
N
Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Fucharoen
Goonnapa
G
Sanchaisuriya
Kanokwan
K
Fucharoen
Supan
S
D004909:Erythrocyte Indices; D006400:Hematocrit; D006446:Hemoglobin E / Q000235:genetics; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008403:Mass Screening; D016133:Polymerase Chain Reaction; D013785:Thailand / Q000453:epidemiology; D017085:alpha-Thalassemia / Q000097:blood* / Q000453:epidemiology / Q000235:genetics
D006446:Hemoglobin E