title: Development of glass-ceramic scaffolds for bone tissue engineering: characterisation, proliferation of human osteoblasts and nodule formation.
abstract: Glass-ceramic macroporous scaffolds for tissue engineering have been developed using a polyurethane sponge template and bioactive glass powders. The starting glass (CEL2) belongs to the system SiO(2)-P(2)O(5)-CaO-MgO-Na(2)O-K(2)O and has been synthesised by a conventional melting-quenching route. A slurry of CEL2 powder, polyvinyl alcohol and water has been prepared in order to coat, by impregnation, the polymeric template. An optimised thermal treatment was then use to remove the sponge and to sinter the glass powders, leading to a glass-ceramic replica of the template. Morphological observations, image analyses, mechanical tests and in vitro tests showed that the obtained devices are good candidates as scaffolds for bone-tissue engineering, in terms of pore-size distribution, pore interconnection, surface roughness, and both bioactivity and biocompatibility. In particular, a human osteoblast cell line (MG-63) seeded onto the scaffold after a standardised preconditioning route in simulated body fluid showed a high degree of cell proliferation and a good ability to produce calcium nodules. The obtained results were enhanced by the addition of bone morphogenetic proteins after cell seeding.
authors.affiliation: Materials Science and Chemical Engineering Department, Polytechnic of Turin, Italy. chiara.vitale@polito.it
authors.forename: C
authors.initials: C
authors.lastname: Vitale-Brovarone
authors.forename: E
authors.initials: E
authors.lastname: Verné
authors.forename: L
authors.initials: L
authors.lastname: Robiglio
authors.forename: P
authors.initials: P
authors.lastname: Appendino
authors.forename: F
authors.initials: F
authors.lastname: Bassi
authors.forename: G
authors.initials: G
authors.lastname: Martinasso
authors.forename: G
authors.initials: G
authors.lastname: Muzio
authors.forename: R
authors.initials: R
authors.lastname: Canuto
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D002516:Ceramics / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D011142:Polyvinyl Alcohol / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta; D011142:Polyvinyl Alcohol