title: Leakage along apical root fillings with and without smear layer using two different leakage models: a two-month longitudinal ex vivo study.
abstract: OBJECTIVE
  To compare two different experimental models when measuring leakage along root fillings with or without smear layer.
  
  
  METHODS
  One hundred and twenty single-rooted teeth were prepared to size 50 and allocated to two groups: fluid transport model (n = 60) and glucose penetration model (n = 60). The roots in each group were divided into three subgroups of 20 teeth each. Smear layer was left in place in group 1 but removed in groups 2 and 3. In groups 1 and 2 canals were filled with laterally compacted gutta-percha cones and AH 26. Group 3 was laterally compacted with Resilon cones and Epiphany sealer. The coronal portion of the filling was removed to assure only 4 mm of filling remained in the canal. Leakage of glucose was evaluated by measuring its concentration once a week for a total period of 56 days using a glucose penetration model. Fluid transport was evaluated by measuring the movement of an air-bubble using a fluid transport model, 1 and 8 weeks after canal filling. Differences between the groups in glucose concentrations and fluid transport were statistically analysed with the Kruskal-Wallis and the Mann-Whitney tests. The level of significance was set at alpha = 0.05.
  
  
  RESULTS
  Glucose penetration was significantly different between the three groups after the first 8 days (P < 0.05). Resilon leaked the most throughout the experiment period. No significant difference (P > 0.05) existed between the two gutta-percha groups at all time intervals (Mann-Whitney test). In the fluid transportation model, no statistically significant differences were observed between all three experimental groups (P > 0.05) at either 1 or 8 weeks after filling (Kruskal-Wallis test).
  
  
  CONCLUSIONS
  Under the conditions of this study, the glucose penetration model was more sensitive in detecting leakage along root fillings. Removing the smear layer before filling did not improve the sealing of the apical 4 mm of filling. Resilon allowed more glucose penetration but the same amount of fluid transport as the gutta-percha root fillings.
authors.affiliation: Department of Cariology Endodontology Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands. h.shemesh@acta.nl
authors.forename: H
authors.initials: H
authors.lastname: Shemesh
authors.forename: M-K
authors.initials: MK
authors.lastname: Wu
authors.forename: P R
authors.initials: PR
authors.lastname: Wesselink
mesh_terms: D003763:Dental Leakage; D005947:Glucose / Q000032:analysis; D006801:Humans; D008137:Longitudinal Studies; D012387:Root Canal Filling Materials / Q000737:chemistry*; D012389:Root Canal Obturation / Q000379:methods; D016369:Smear Layer; D018709:Statistics, Nonparametric
chemical_list: D012387:Root Canal Filling Materials; C486892:resilon sealer; D005947:Glucose