Both trivalent and hexavalent chromium strongly alter in vitro germination and ultrastructure of kiwifruit pollen.
Due to its widespread industrial use, chromium is considered a dangerous environmental pollutant. It is known to inhibit plant growth and development. The present study provides the first evidence of the toxicity of this metal on the male haploid generation of a higher plant. Both Cr(III) and Cr(VI) species, supplied as CrCl(3) and CrO(3), respectively, exerted a strong dose-dependent inhibitory effect on kiwifruit pollen tube emergence and growth. Cr(III) resulted more effective than Cr(VI) in the 16-75microM interval; moreover, complete inhibition of germination was attained at much lower doses than Cr(VI). Also tube morphology was affected. While the plasma membrane was still undamaged in the large majority of the treated pollen grains, dramatic ultrastructural alterations were induced by chromium including chromatin condensation, swelling of mitochondria, cytoplasmic vacuolization, and perturbed arrangement of endoplasmic reticulum cisternae. Thus, it seems that the impact of the two chromium species on kiwifruit pollen may result in severe compromission of both essential structures and functions of the male gametophyte.
Speranza
Anna
A
Dipartimento di Biologia Evoluzionistica Sperimentale, Università di Bologna, via Irnerio 42, 40126 Bologna, Italy. anna.speranza@unibo.it
Ferri
Paola
P
Battistelli
Michela
M
Falcieri
Elisabetta
E
Crinelli
Rita
R
Scoccianti
Valeria
V
D029042:Actinidia / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure; D002712:Chlorides / Q000633:toxicity*; D017608:Chromium Compounds / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity*; D018525:Germination / Q000187:drug effects*; D046529:Microscopy, Electron, Transmission; D011058:Pollen / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure
D002712:Chlorides; D017608:Chromium Compounds; D004785:Environmental Pollutants; C028801:chromium trioxide; C017133:chromous chloride