title: Parenteral administration of glutamine modulates acute phase response in postparturient dairy cows.
abstract: The objective of this study was to investigate whether administration of L-Gln would affect mediators of acute phase response in postparturient dairy cows. Twenty-four multiparous Holstein cows were blocked by the expected day of calving and randomly assigned to 1 of the 3 treatment groups (n = 8/group): 1) i.v. infusion of 10 L of 0.85% NaCl (control), 2) i.v. infusion of 106, or 3) 212 g/d of L-Gln mixed with 10 L of 0.85% NaCl solution; each treatment was given 8 h/d for each of 7 consecutive days starting on d 1 after calving. Blood samples were collected 1 wk before the expected day of parturition as well as on d 0, 7, 14, and 21 after parturition; plasma concentrations of serum amyloid A (SAA), haptoglobin, and lipopolysaccharide-binding protein were measured by ELISA, and alpha(1)-acid glycoprotein was assessed by radial immunodiffusion. Concentrations of SAA, haptoglobin, and alpha(1)-acid glycoprotein increased in control cows after parturition, reaching peak values on d 0 or 7 postpartum (60, 1,093, and 963 microg/mL, respectively). Cows infused with 106 g/d of L-Gln had greater concentrations of SAA in plasma on d 14 and 21 compared with controls (62.8 vs. 30.2 and 71.1 vs. 34.5 microg/mL, respectively). Cows infused with 212 g/d of L-Gln had greater concentrations of SAA on d 7 (82.5 vs. 53.9 microg/mL) and lower concentrations of haptoglobin on d 14 and 21 postpartum compared with controls (264 vs. 621 and 175 vs. 587 microg/mL, respectively). Cows treated with 106 and 212 g/d of L-Gln had greater plasma lipopolysaccharide-binding protein concentrations on d 7 compared with control group (50.0 and 35.6 vs. 10.8 microg/mL, respectively). There were no treatment differences with respect to milk yield and DM intake during the experimental period. In conclusion, our data indicate that i.v. administration of L-Gln modulated acute phase mediators in dairy cows after parturition and warrants further research into the mechanisms behind these effects.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada T6G 2P5.
authors.forename: A
authors.initials: A
authors.lastname: Jafari
authors.forename: D G V
authors.initials: DG
authors.lastname: Emmanuel
authors.forename: R J
authors.initials: RJ
authors.lastname: Christopherson
authors.forename: J R
authors.initials: JR
authors.lastname: Thompson
authors.forename: G K
authors.initials: GK
authors.lastname: Murdoch
authors.forename: J
authors.initials: J
authors.lastname: Woodward
authors.forename: C J
authors.initials: CJ
authors.lastname: Field
authors.forename: B N
authors.initials: BN
authors.lastname: Ametaj
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis / Q000187:drug effects*; D000210:Acute-Phase Reaction / Q000276:immunology / Q000662:veterinary*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002417:Cattle / Q000276:immunology*; D003612:Dairying / Q000379:methods*; D004032:Diet / Q000662:veterinary; D004435:Eating / Q000187:drug effects; D005260:Female; D005973:Glutamine / Q000008:administration & dosage / Q000494:pharmacology*; D007263:Infusions, Parenteral / Q000662:veterinary; D007774:Lactation / Q000187:drug effects; D016018:Least-Squares Analysis; D049590:Postpartum Period; D011247:Pregnancy; D011897:Random Allocation; D013997:Time Factors
chemical_list: D000209:Acute-Phase Proteins; D005973:Glutamine