Characterization of Osh3, an oxysterol-binding protein, in filamentous growth of Saccharomyces cerevisiae and Candida albicans.
OSH3 is one of the seven yeast homologues of the oxysterol binding proteins (OSBPs) which have the major binding affinity to the oxysterols and function as regulator of cholesterol biosynthesis in mammals. Mutational analysis of OSH3 showed that OSH3 plays a regulatory role in the yeast-to-hyphal transition through its oxysterol-binding domain in Saccharomyces cerevisiae. The OSH3 gene was also identified in the pathogenic yeast Candida albicans. Deletion of OSH3 caused a defect in the filamentous growth, which is the major cause of the C. albicans pathogencity. The filamentation defect of the mutation in the MAPK-associated transcription factor, namely cph1Delta was suppressed by overexpression of OSH3. These findings suggest the regulatory roles of OSH3 in the yeast filamentous growth and the functional conservations of OSH3 in S. cerevisiae and C. albicans.
Hur
Hyang-sook
HS
Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University,Daejeon 305-764, Republic of Korea.
Ryu
Ji-Ho
JH
Kim
Kwang-Hoon
KH
Kim
Jinmi
J
D002176:Candida albicans / Q000235:genetics* / Q000254:growth & development; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D008957:Models, Genetic; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D011987:Receptors, Steroid / Q000235:genetics* / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics; D000098825:Oxysterol Binding Proteins
D005656:Fungal Proteins; D011987:Receptors, Steroid; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000098825:Oxysterol Binding Proteins