Altered gene expression of caspase-10, death receptor-3 and IGFBP-3 in preeclamptic placentas.
Enhanced apoptosis has been observed in the placentas of women with preeclampsia, but few studies have examined changes at the molecular level. This study was designed to detect genes specifically expressed in full-term preeclamptic placentas. Tissue samples were collected immediately after cesarean delivery from 11 normal and 8 preeclamptic placentas at 35-40 weeks of gestation. Total RNAs were extracted and hybridized to a cDNA microarray. Results were confirmed by reverse-transcription polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry. Hematoxylin and eosin and TUNEL staining were also performed to confirm apoptosis in preeclamptic placentas. Among 205 genes, three were up- or down-regulated in preeclamptic placentas. The expression of caspase-10 and death receptor 3 (DR-3) was significantly increased, whereas insulin-like growth factor binding protein-3 (IGFBP-3) was strongly down-regulated. RT-PCR analysis and Western blotting confirmed these effects. Immunohistochemical analysis showed that the DR-3, caspase-10 and IGFBP-3 proteins were localized in the syncytial membrane. Apoptosis in the trophoblast was also increased in term placentas from women with pregnancies complicated by preeclampsia. These results suggest that caspase-10, DR-3 and IGFBP-3 are involved in apoptosis in the preeclamptic placenta.
Han
Jae Yoon
JY
Department of Anatomy and Neurobiology, Institute of Health Science, Gyeongsang National University, Jinju 660-751, Korea.
Kim
Yoon Sook
YS
Cho
Gyeong Jae
GJ
Roh
Gu Seob
GS
Kim
Hyun Joon
HJ
Choi
Won Jun
WJ
Paik
Won Young
WY
Rho
Gyu Jin
GJ
Kang
Sang Soo
SS
Choi
Wan Sung
WS
D017209:Apoptosis / Q000235:genetics; D053455:Caspase 10 / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D011225:Pre-Eclampsia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D053219:Receptors, Tumor Necrosis Factor, Member 25 / Q000235:genetics*; D015854:Up-Regulation
D018972:Insulin-Like Growth Factor Binding Protein 3; D053219:Receptors, Tumor Necrosis Factor, Member 25; D053455:Caspase 10