title: Fortuitous structure determination of 'as-isolated' Escherichia coli bacterioferritin in a novel crystal form.
abstract: Escherichia coli bacterioferritin was serendipitously crystallized in a novel cubic crystal form and its structure could be determined to 2.5 A resolution despite a high degree of merohedral twinning. This is the first report of crystallographic data on 'as-isolated' E. coli bacterioferritin. The ferroxidase active site contains positive difference density consistent with two metal ions that had co-purified with the protein. X-ray fluorescence studies suggest that the metal composition is different from that of previous structures and is a mix of zinc and native iron ions. The ferroxidase-centre configuration displays a similar flexibility as previously noted for other bacterioferritins.
authors.affiliation: Laboratory of Biophysical Chemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
authors.forename: André
authors.initials: A
authors.lastname: van Eerde
authors.forename: Suzanne
authors.initials: S
authors.lastname: Wolterink-van Loo
authors.forename: John
authors.initials: J
authors.lastname: van der Oost
authors.forename: Bauke W
authors.initials: BW
authors.lastname: Dijkstra
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002570:Ceruloplasmin / Q000737:chemistry / Q000302:isolation & purification; D003300:Copper / Q000032:analysis; D018360:Crystallography, X-Ray / Q000379:methods; D003573:Cytochrome b Group / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000201:enzymology; D005293:Ferritins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D007501:Iron / Q000032:analysis; D008345:Manganese / Q000032:analysis; D008958:Models, Molecular; D011487:Protein Conformation; D015032:Zinc / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; D008345:Manganese; D003300:Copper; D005293:Ferritins; C018032:bacterioferritin; D007501:Iron; D002570:Ceruloplasmin; D015032:Zinc