title: Pathway-specific differences between tumor cell lines and normal and tumor tissue cells.
abstract: BACKGROUND
  Cell lines are used in experimental investigation of cancer but their capacity to represent tumor cells has yet to be quantified. The aim of the study was to identify significant alterations in pathway usage in cell lines in comparison with normal and tumor tissue.
  
  
  METHODS
  This study utilized a pathway-specific enrichment analysis of publicly accessible microarray data and quantified the gene expression differences between cell lines, tumor, and normal tissue cells for six different tissue types. KEGG pathways that are significantly different between cell lines and tumors, cell lines and normal tissues and tumor and normal tissue were identified through enrichment tests on gene lists obtained using Significance Analysis of Microarrays (SAM).
  
  
  RESULTS
  Cellular pathways that were significantly upregulated in cell lines compared to tumor cells and normal cells of the same tissue type included ATP synthesis, cell communication, cell cycle, oxidative phosphorylation, purine, pyrimidine and pyruvate metabolism, and proteasome. Results on metabolic pathways suggested an increase in the velocity nucleotide metabolism and RNA production. Pathways that were downregulated in cell lines compared to tumor and normal tissue included cell communication, cell adhesion molecules (CAMs), and ECM-receptor interaction. Only a fraction of the significantly altered genes in tumor-to-normal comparison had similar expressions in cancer cell lines and tumor cells. These genes were tissue-specific and were distributed sparsely among multiple pathways.
  
  
  CONCLUSIONS
  Significantly altered genes in tumors compared to normal tissue were largely tissue specific. Among these genes downregulation was a major trend. In contrast, cell lines contained large sets of significantly upregulated genes that were common to multiple tissue types. Pathway upregulation in cell lines was most pronounced over metabolic pathways including cell nucleotide metabolism and oxidative phosphorylation. Signaling pathways involved in adhesion and communication of cultured cancer cells were downregulated. The three way pathways comparison presented in this study brings light into the differences in the use of cellular pathways by tumor cells and cancer cell lines.
authors.affiliation: Center for Integrated Bioinformatics, School of Biomedical Engineering, Science and Health Systems, Bossone 714, Drexel University, 3143 Chestnut Street, Philadelphia, PA 19104, USA. ame28@drexel.edu
authors.forename: Adam
authors.initials: A
authors.lastname: Ertel
authors.forename: Arun
authors.initials: A
authors.lastname: Verghese
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Byers
authors.forename: Michael
authors.initials: M
authors.lastname: Ochs
authors.forename: Aydin
authors.initials: A
authors.lastname: Tozeren
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D030541:Databases, Genetic; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015398:Signal Transduction / Q000235:genetics* / Q000502:physiology; D014407:Tumor Cells, Cultured