title: Elucidation of carotenoid patterns in citrus products by means of comprehensive normal-phase x reversed-phase liquid chromatography.
abstract: A novel approach for carotenoid analysis has been developed. Orange essential oil and juice carotenoids were separated by means of comprehensive dual-gradient elution HPLC, using normal phase with a microbore silica column in the first dimension (first D), reversed phase with a monolithic C18 column in the second dimension (second D), and a 10-port switching valve as an interface. An on-line photodiode array detector was used in order to obtain absorption spectra. Peak identification was obtained by combining retention data with the UV-visible spectra.
authors.affiliation: Dipartimento di Scienze degli Alimenti e dell'Ambiente, Facoltà di Scienze MM.FF.NN., Università di Messina, Salita Sperone, 98166 Messina, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.forename: Veronika
authors.initials: V
authors.lastname: Skeriková
authors.forename: Tiina
authors.initials: T
authors.lastname: Kumm
authors.forename: Alessandra
authors.initials: A
authors.lastname: Trozzi
authors.forename: Pavel
authors.initials: P
authors.lastname: Jandera
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
mesh_terms: D001628:Beverages / Q000032:analysis*; D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D002338:Carotenoids; D012822:Silicon Dioxide