title: Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative transposase from Thermoplasma acidophilum encoded by the Ta0474 gene.
abstract: IS200 transposases, originally identified in Salmonella typhimurium LT2, are present in many bacteria and archaea and are distinct from other groups of transposases. To facilitate further structural comparisons among IS200-like transposases, structural analysis has been initiated of a putative transposase from Thermoplasma acidophilum encoded by the Ta0474 gene. Its 137-residue polypeptide shows high levels of sequence similarity to other members of the IS200 transposase family. The protein was overexpressed in intact form in Escherichia coli and crystallized at 297 K using a reservoir solution consisting of 100 mM Na HEPES pH 7.5 and 20%(v/v) ethanol. X-ray diffraction data were collected to 1.78 A. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 65.00, b = 34.07, c = 121.58 A, alpha = 90, beta = 100.20, gamma = 90 degrees. Four monomers, representing two copies of a dimeric molecule, are present in the asymmetric unit, giving a crystal volume per protein weight (V(M)) of 2.02 A(3) Da(-1) and a solvent content of 39.2%.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Ji Yong
authors.initials: JY
authors.lastname: Kang
authors.forename: Hyung Ho
authors.initials: HH
authors.lastname: Lee
authors.forename: Do Jin
authors.initials: DJ
authors.lastname: Kim
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Han
authors.forename: Olesya
authors.initials: O
authors.lastname: Kim
authors.forename: Hyoun Sook
authors.initials: HS
authors.lastname: Kim
authors.forename: Sang Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Se Won
authors.initials: SW
authors.lastname: Suh
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry; D001483:Base Sequence; D018360:Crystallography, X-Ray; D017931:DNA Primers; D019766:Genes, Archaeal; D008969:Molecular Sequence Data; D013822:Thermoplasma / Q000235:genetics*; D019895:Transposases / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D019843:Archaeal Proteins; D017931:DNA Primers; D019895:Transposases