[Significance of hepatitis B virus PreS1-Ag, PreS2-Ag, large protein, PreS2-Ab detection and the prediction of HBV DNA replication.].
BACKGROUND
To explore the significance of hepatitis B virus PreS1-Ag, PreS2-Ag, large protein (LP) detection and the prediction of viral replication.


METHODS
PreS1-Ag, PreS2-Ag, LP and HBV markers were measured by enzyme linked immunosorbent assay (ELISA) in 201 cases of infected serum. Serum HBV DNA level was quantitatively detected by real-time polymerase chain reaction (PCR).


RESULTS
There were significant differences in positive rate between the PreS1-Ag, PreS2-Ag, LP, and HBsAg; the positive rate of PreS2-Ag and LP were higher than that of the HBeAg. No significant differences were found in the positive rates between LP and the levels of HBV DNA and there was a positive correlation between quantitations of HBV DNA and HBV-LP.


CONCLUSIONS
Serum PreS1-Ag, PreS2-Ag and LP were laboratory markers that can accurately reflect HBV DNA reproduction, and were helpful complementarity to traditional HBV M. There is a close correlation between the number of copies of HBV DNA and the levels of HBV-LP.
Mao
Y L
YL
Center of Clinical Laboratory, The No. 302 Hospital of The People's Liberation Army, Beijing 100039, China.
Li
B A
BA
Ma
H B
HB
Sun
Z Q
ZQ
Shi
J B
JB
Li
X H
XH
Xu
J
J
Wang
X F
XF
Yang
L H
LH
D004261:DNA Replication; D004279:DNA, Viral / Q000097:blood; D006509:Hepatitis B / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D011498:Protein Precursors / Q000097:blood
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors