Overexpression of an Arabidopsis magnesium transport gene, AtMGT1, in Nicotiana benthamiana confers Al tolerance.
Aluminium (Al) toxicity is the most important limiting factor for crop production in acid soil environments worldwide. In some plant species, application of magnesium (Mg(2+)) can alleviate Al toxicity. However, it remains unknown whether overexpression of magnesium transport proteins can improve Al tolerance. Here, the role of AtMGT1, a member of the Arabidopsis magnesium transport family involved in Mg(2+) transport, played in Al tolerance in higher plants was investigated. Expression of 35S::AtMGT1 led to various phenotypic alterations in Nicotiana benthamiana plants. Transgenic plants harbouring 35S::AtMGT1 exhibited tolerance to Mg(2+) deficiency. Element assay showed that the contents of Mg, Mn, and Fe in 35S::AtMGT1 plants increased compared with wild-type plants. Root growth experiment revealed that 100 microM AlCl(3) caused a reduction in root elongation by 47% in transgenic lines, whereas root growth in wild-type plants was inhibited completely. Upon Al treatment, representative transgenic lines also showed a much lower callose deposition, an indicator of increased Al tolerance, than wild-type plants. Taken together, the results have demonstrated that overexpression of ATMGT1 encoding a magnesium transport protein can improve tolerance to Al in higher plants.
Deng
Wei
W
Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, Chongqing 400716, China.
Luo
Keming
K
Li
Demou
D
Zheng
Xuelian
X
Wei
Xiaoyang
X
Smith
William
W
Thammina
Chandra
C
Lu
Litang
L
Li
Yi
Y
Pei
Yan
Y
D000535:Aluminum / Q000378:metabolism / Q000633:toxicity*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D017796:Caulimovirus / Q000235:genetics; D005936:Glucans / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D010641:Phenotype; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D029681:Arabidopsis Proteins; D027682:Cation Transport Proteins; D005936:Glucans; C517535:MGT1 protein, Arabidopsis; D008345:Manganese; C048306:callose; D000535:Aluminum; D007501:Iron; D008274:Magnesium