title: A new technique for producing recombinant baculovirus directly in silkworm larvae.
abstract: A new technique for the direct production of recombinant baculovirus in the silkworm larvae is described. To assess the utility of this method, a combination of Bombyx mori nucleopolyhedroviral genome, transfer vector and Lipofectin was co-injected directly into newly ecdysed fifth instar, silkworm larvae. The recombinant virus was obtained from the hemolymph of injected larvae and the hemolymph then re-injected into the larvae as an inoculum. This resulted in a high-level production of foreign protein in the silkworm larvae. This technique produces easy and rapid recombinant protein production in silkworms.
authors.affiliation: College of Natural Resources and Life Science, Dong-A University, Busan, Korea. brjin@dau.ac.kr
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Lee
authors.forename: Bo Yeon
authors.initials: BY
authors.lastname: Kim
authors.forename: Yeon Ho
authors.initials: YH
authors.lastname: Je
authors.forename: Soo Dong
authors.initials: SD
authors.lastname: Woo
authors.forename: Hung Dae
authors.initials: HD
authors.lastname: Sohn
authors.forename: Byung Rae
authors.initials: BR
authors.lastname: Jin
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D012831:Bombyx / Q000235:genetics* / Q000821:virology*; D024861:Genetic Enhancement / Q000379:methods; D007814:Larva / Q000235:genetics / Q000821:virology; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014162:Transfection / Q000379:methods*; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D011994:Recombinant Proteins