[Study of the efficacy of a monoclonal antibody biotin-avidin system for the diagnosis of schistosomiasis japonica].
OBJECTIVE
To explore the diagnostic efficiency of circulating antigen using the TM5.28 mAB-biotin-avidin system for the detection of schistosomiasis japonica.


METHODS
A mAb-biotin-avidin system was set up using a TM5.28 mAB which was prepared against a gut associated antigen of Schistosoma japonicum. Detection was performed on the sera from 50 acute schistosomiasis patients, 224 chronic patients, 49 advanced patients and 46 schistosomiasis patients who were followed up at 6 months and 12 months post treatment. In addition, 19 cases of clonorchiasis, 31 cases of paragonimiasis, 23 cases of hepatitis B and 100 healthy individuals were also included.


RESULTS
The system showed sensitivity of 83.1% and specificity of 94.0% when applied to detect chronic schistosomiasis and healthy persons respectively, while 94.0% to acute schistosomiasis. The Youden's index of the system was 0.771. The rate of cross-reaction to paragonimiasis, clonorchiasis and hepatitis B was 12.9%, 15.8% and 13.0% respectively. The rates of negative turning were 43.9% and 62.1% respectively in chronic schistosomiasis at the 6 month and 12 month intervals after treatment. Geometric mean of the OD values also decreased from 0.172 before treatment to 0.081 at 6 months and 0.068 at 12 months after treatment with a reduction rate of 60.30%. The detection rate in the heavy infected population reached a maximum of 90.0%. This was similar in moderate and light infected populations, i.e., 83.9% and 82.1%, respectively.


CONCLUSIONS
The TM5.28 mAb-biotin-avidin system showed a relatively high efficiency in the diagnosis of schistosomiasis and a high negative turning rate after treatment. It is, therefore, a valuable tool for the estimation of prevalence in endemic populations, as well as individual diagnosis and for assessing the effect of chemotherapy.
Chen
Hong-gen
HG
Department of Diagnosis, Jiangxi Provincial Institute of Parasitic Diseases, Nanchang 330046, China.
Zeng
Xiao-jun
XJ
Ge
Jun
J
Jiang
Wei-sheng
WS
Kikuchi
Mihoko
M
Hirayama
Kenji
K
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001360:Avidin / Q000276:immunology; D001710:Biotin / Q000276:immunology; D002459:Cell Fusion; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D012554:Schistosomiasis japonica / Q000175:diagnosis* / Q000276:immunology; D012698:Serologic Tests
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin