A newly isolated organic solvent tolerant Staphylococcus saprophyticus M36 produced organic solvent-stable lipase.
Thirty-eight high lipase activity strains were isolated from soil, seawater, and Brassica napus. Among them, a novel organic solvent tolerant bacterium (strain M36) was isolated from the seawater in Jiangsu, China. Isolate M36 was able to grow at high concentration of benzene or toluene up to 40% (vol/vol), and later identified as Staphylococcus saprophyticus by biochemical test and 16s ribosomal DNA sequence. No work on Staphylococcus producing lipase with organic solvent tolerance has been reported so far. The lipase of strain M36 whose activity in liquid medium was 42 U mL(-1) at 24-h incubation time was stable in the presence of 25% (vol/vol) p-xylene, benzene, toluene, and hexane.
Fang
Yaowei
Y
College of Food Science and Technology, Nanjing Agriculture University, Nanjing, China.
Lu
Zhaoxin
Z
Lv
Fengxia
F
Bie
Xiaomei
X
Liu
Shu
S
Ding
Zhongyang
Z
Xu
Weifeng
W
D001554:Benzene / Q000378:metabolism / Q000494:pharmacology; D029688:Brassica napus / Q000382:microbiology; D002681:China; D004361:Drug Tolerance; D004795:Enzyme Stability; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology; D012988:Soil Microbiology; D012997:Solvents / Q000378:metabolism* / Q000494:pharmacology*; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification*
D012997:Solvents; D008049:Lipase; D001554:Benzene