title: Nosocomial outbreak of Pseudomonas aeruginosa endophthalmitis.
abstract: We describe an outbreak of nosocomial endophthalmitis due to a common source, which was determined to be trypan blue solution prepared in the hospital's pharmacy service. We assume that viable bacteria probably gained access to the trypan blue stock solution during cooling after autoclaving. The temporal cluster of Pseudomonas aeruginosa endophthalmitis was readily perceived on the basis of clinical and microbiological findings, and an exogenous source of contamination was unequivocally identified by means of DNA fingerprinting.
authors.affiliation: Servicio de Microbiologia, Hospitales Universitarios Virgen del Rocio, Sevilla, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Mateos
authors.forename: R
authors.initials: R
authors.lastname: Valencia
authors.forename: M J
authors.initials: MJ
authors.lastname: Torres
authors.forename: A
authors.initials: A
authors.lastname: Cantos
authors.forename: M
authors.initials: M
authors.lastname: Conde
authors.forename: J
authors.initials: J
authors.lastname: Aznar
mesh_terms: D000368:Aged; D002021:Buffers; D002386:Cataract / Q000175:diagnosis; D004396:Coloring Agents / Q000008:administration & dosage; D003428:Cross Infection / Q000453:epidemiology* / Q000209:etiology / Q000382:microbiology; D016172:DNA Fingerprinting / Q000379:methods; D004196:Disease Outbreaks; D009877:Endophthalmitis / Q000453:epidemiology* / Q000209:etiology / Q000382:microbiology; D004866:Equipment Contamination; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000209:etiology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014343:Trypan Blue / Q000008:administration & dosage
chemical_list: D002021:Buffers; D004396:Coloring Agents; D014343:Trypan Blue