title: [Preparation of antibody against Memo protein and analysis of expression profile of Memo in mouse tissues].
abstract: OBJECTIVE
  To prepare and characterize antibody against Memo protein and to detect the tissue distribution of Memo in mice.
  
  
  METHODS
  Fusion protein GST-Memo was expressed and purified, and polyclonal antibody against Memo was prepared by immunizing mice. A FLAG-tagged eukaryotic expression vector pcDNA3-FLAG-Memo was constructed. The specificity of the antibody was detected by Western blot.
  
  
  RESULTS
  An eukaryotic expression vector pcDNA3-FLAG-Memo was obtained. The polyclonal antibody was found to be specific to Memo. Memo protein was widely expressed in mouse tissues using the obtained antibody in Western blot.
  
  
  CONCLUSIONS
  Antibody specific to Memo has been successfully obtained, which provides useful tool for investigation into Memo-associated mechanisms of tumor metastasis and invasiveness.
authors.affiliation: Institute of Bioengineering, Academy of Military Medical Sciences, Beijing 100850, China. ningkangnk@163.com
authors.forename: Kang
authors.initials: K
authors.lastname: Ning
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Ju-Qiang
authors.initials: JQ
authors.lastname: Han
authors.forename: Hong-Xue
authors.initials: HX
authors.lastname: Fan
authors.forename: Jie-Zhi
authors.initials: JZ
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Qi-Nong
authors.initials: QN
authors.lastname: Ye
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D051379:Mice; D019159:Nonheme Iron Proteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*; D009928:Organ Specificity; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000906:Antibodies; C540429:Memo protein, mouse; D019159:Nonheme Iron Proteins; D011993:Recombinant Fusion Proteins