title: Concatenated logic gates using four coupled biocatalysts operating in series.
abstract: The assembly of three concatenated enzyme-based logic gates consisting of OR, AND, XOR is described. Four biocatalysts, acetylcholine esterase, choline oxidase, microperoxidase-11, and the NAD+-dependent glucose dehydrogenase, are used to assemble the gates. Four inputs that include acetylcholine, butyrylcholine, O2, and glucose are used to drive the concatenated-gates system. The cofactor NAD+, and its reduced 1,4-dihydro form, NADH, are used as a reporter couple, and these provide an optical output for the gates. The modulus of the absorbance changes of NADH is used as a readout signal.
authors.affiliation: Institute of Chemistry, Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors.forename: Tamara
authors.initials: T
authors.lastname: Niazov
authors.forename: Ronan
authors.initials: R
authors.lastname: Baron
authors.forename: Eugenii
authors.initials: E
authors.lastname: Katz
authors.forename: Oleg
authors.initials: O
authors.lastname: Lioubashevski
authors.forename: Itamar
authors.initials: I
authors.lastname: Willner
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D045304:Cytochromes c / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism; D009243:NAD / Q000737:chemistry* / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism
chemical_list: D009243:NAD; D045304:Cytochromes c; D000429:Alcohol Oxidoreductases; D042843:Glucose 1-Dehydrogenase; C019888:choline oxidase; D010544:Peroxidases; C021799:microperoxidase; D000110:Acetylcholinesterase