Expression and RNA interference-induced silencing of the dammarenediol synthase gene in Panax ginseng.
Panax ginseng is one of the most highly valued herbal medicines in the Orient, where it has gained an almost magical reputation for being able to maintain the quality of life. The root of ginseng contains noble tetracyclic triterpenenoid saponins, which are thought to be the major effective ingredients in P. ginseng. The first committed step in ginsenoside synthesis is the cyclization of 2,3-oxidosqualene to dammarenediol II by oxidosqualene cyclase, dammarenediol synthase (DDS). The gene encoding DDS has been characterized. Here, we investigated the expression of the DDS gene together with the genes involved in ginsenoside biosynthesis (SS, SE, PNX, PNY, PNY2 and PNZ). Expression of DDS mRNA was higher in flower buds compared with root, leaf and petiole of ginseng plants. Elicitor (methyl jasmonate) treatment up-regulated the expression of DDS mRNA. Ectopic expression of DDS in a yeast mutant (erg7) lacking lanosterol synthase resulted in the production of dammarenediol and hydroxydammarenone which were confirmed by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (LC/APCIMS). RNA interference (RNAi) of DDS in transgenic P. ginseng resulted in silencing of DDS expression which leads to a reduction of ginsenoside production to 84.5% in roots. These results indicate that expression of DDS played a vital role in the biosynthesis of ginsenosides in P. ginseng.
Han
Jung Yeon
JY
College of Pharmacy, Kangwon National University, Chunchon 200-701, Republic of Korea.
Kwon
Yong Soo
YS
Yang
Deok Chun
DC
Jung
Young Rim
YR
Choi
Yong Eui
YE
D000085:Acetates / Q000494:pharmacology; D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D036145:Ginsenosides / Q000096:biosynthesis; D008969:Molecular Sequence Data; D054883:Oxylipins; D005894:Panax / Q000201:enzymology* / Q000235:genetics*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics
D000085:Acetates; D003517:Cyclopentanes; D018076:DNA, Complementary; D036145:Ginsenosides; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; C072239:methyl jasmonate; D019883:Alkyl and Aryl Transferases; C514651:dammarenediol-II synthase, Panax ginseng