Molecular characterization of miniature porcine RANTES and its chemotactic effect on human mononuclear cells.
To elucidate the potential role of porcine RANTES (Regulated upon Activation Normal T cells Expressed and Secreted) in xenograft rejection, we investigated its chemotactic activity for human mononuclear cells, as well as the effect of human cytokines on its expression in porcine endothelial cells. Porcine RANTES cDNA was successfully cloned from aortic endothelial cells of miniature pigs, and its protein expression was induced by transfection. Its deduced amino acid sequence was 83.5% identical to that of human RANTES. Porcine RANTES triggered transmigration of human mononuclear cells across the species barrier, and this chemotactic effect was suppressed by anti-RANTES neutralizing antibodies. The chemotactic effect of porcine RANTES was most prominent on human monocytes. Human tumor necrosis factor-alpha induced significant expression of porcine RANTES messenger RNA in endothelial cells; however both human interferon-gamma and interleukin-1beta failed. These results suggest that porcine RANTES can play an important role in xenotransplant rejection, through participating in the interaction between porcine endothelial cells and human monocytes.
Yang
Jaeseok
J
Transplantation Research Institute, Seoul National University College of Medicine, Seoul, Korea.
Cho
Bumrae
B
Choi
Inho
I
Kim
Dong Hee
DH
Kim
Sung Dae
SD
Koh
Hyun Sook
HS
Ro
Han
H
Oh
Kook Hwan
KH
Chung
Junho
J
Kim
Jae Young
JY
Ahn
Curie
C
Kim
Suhnggwon
S
Lee
Jung Sang
JS
D000595:Amino Acid Sequence; D000818:Animals; D018946:Chemokine CCL5 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000235:genetics; D016207:Cytokines / Q000494:pharmacology; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000187:drug effects / Q000276:immunology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D013552:Swine
D018946:Chemokine CCL5; D016207:Cytokines; D012333:RNA, Messenger