title: High resolution tiling-path BAC array deletion mapping suggests commonly involved 3p21-p22 tumor suppressor genes in neuroblastoma and more frequent tumors.
abstract: The recurrent loss of 3p segments in neuroblastoma suggests the implication of 1 or more tumor suppressor genes but thus far few efforts have been made to pinpoint their detailed chromosomal position. To achieve this goal, array-based comparative genomic hybridization was performed on a panel of 23 neuroblastoma cell lines and 75 primary tumors using a tiling-path bacterial artificial chromosome array for chromosome 3p. A total of 45 chromosome 3 losses were detected, including whole chromosome losses, large terminal deletions and interstitial deletions. The latter, observed in cell lines as well as a number of distal deletions detected in primary tumors, allowed us to demarcate 3 minimal regions of loss of 3.6 Mb [3p21.31-p21.2, shortest regions of overlap (SRO)1], 1.4 Mb (3p22.3-3p22.2, SRO2) and 3.8 Mb (3p25.3-p25.1, SRO3) in size. The present data significantly extend previous findings and now firmly establish critical regions on 3p implicated in neuroblastoma. Interestingly, the 2 proximal regions coincide with previously defined SROs on 3p21.3 in more frequent tumors including lung and breast cancer. As such, similar tumor suppressor genes may play a critical role in development or progression of a variety of neoplasms, including neuroblastoma.
authors.affiliation: Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium.
authors.forename: Jasmien
authors.initials: J
authors.lastname: Hoebeeck
authors.forename: Evi
authors.initials: E
authors.lastname: Michels
authors.forename: Björn
authors.initials: B
authors.lastname: Menten
authors.forename: Nadine
authors.initials: N
authors.lastname: Van Roy
authors.forename: Angelika
authors.initials: A
authors.lastname: Eggert
authors.forename: Alexander
authors.initials: A
authors.lastname: Schramm
authors.forename: Katleen
authors.initials: K
authors.lastname: De Preter
authors.forename: Nurten
authors.initials: N
authors.lastname: Yigit
authors.forename: Els
authors.initials: E
authors.lastname: De Smet
authors.forename: Anne
authors.initials: A
authors.lastname: De Paepe
authors.forename: Geneviève
authors.initials: G
authors.lastname: Laureys
authors.forename: Jo
authors.initials: J
authors.lastname: Vandesompele
authors.forename: Frank
authors.initials: F
authors.lastname: Speleman
mesh_terms: D045744:Cell Line, Tumor; D002872:Chromosome Deletion; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D020451:Contig Mapping / Q000379:methods; D018450:Disease Progression; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor / Q000502:physiology; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D009693:Nucleic Acid Hybridization / Q000379:methods*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D025521:Tumor Suppressor Proteins