Synergistic effect of TGFbeta-3 on chondrogenic differentiation of rabbit chondrocytes in thermo-reversible hydrogel constructs blended with hyaluronic acid by in vivo test.
In this study, a hydrogel composite, based on the thermo-reversible hydrogel of p(NiPAAm-co-AAc) and hyaluronic acid (HA) was used as an injectable cell and growth factor carrier for cartilage tissue engineering applications. Rabbit chondrocytes were embedded in blended hydrogel composites co-encapsulated with the transforming growth factor beta-3 (TGFbeta-3). The blended hydrogel with the embedded chondrocytes and HA co-encapsulating unloaded growth factors and those with the thermo-reversible hydrogel were used as the controls to examine the effects of TGFbeta-3 on neocartilage formation. The blended hydrogel loaded with TGFbeta-3 embedded with chondrocytes were injected subcutaneously into the nude mice. The mice were monitored for 8 weeks after the injection. Both the differentiation and level of cartilage-specific ECM production were significantly higher in the presence of HA and growth factor than in the control without the growth factor. The level of cartilage associated ECM proteins was examined by immunohistochemical staining (collagen types II and X) as well as by Safranin-O and Alcian blue (GAG) staining. The results showed the potential application of blended hydrogel mixed with the growth factor to neocartilage formation.
Na
Kun
K
Division of Biotechnology, The Catholic University of Korea, 43-1 Yokkok 2-dong, Wonmi-gu, Bucheon 420-743, Republic of Korea.
Kim
Sungwon
S
Woo
Dae-Gyun
DG
Sun
Bo Kyung
BK
Yang
Han Na
HN
Chung
Hyung-Min
HM
Park
Keun-Hong
KH
D000178:Acrylamides / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry; D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000254:growth & development*; D002454:Cell Differentiation / Q000187:drug effects*; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D016207:Cytokines / Q000008:administration & dosage / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism*; D006651:Histocytochemistry; D006820:Hyaluronic Acid / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D051379:Mice; D008819:Mice, Nude; D011108:Polymers / Q000737:chemistry; D011817:Rabbits; D053782:Transforming Growth Factor beta3 / Q000008:administration & dosage / Q000494:pharmacology*
D000178:Acrylamides; D000179:Acrylates; D016207:Cytokines; C090629:N-isopropylacrylamide-acrylic acid copolymer; D011108:Polymers; D053782:Transforming Growth Factor beta3; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006820:Hyaluronic Acid