title: [Inhibitory effect of weikangfu recipe on growth of mouse S180 tumor and its apoptotic induction].
abstract: OBJECTIVE
  To study the growth inhibition of Weikangfu recipe on S180 tumor and its apoptotic induction.
  
  
  METHODS
  S180-bearing mice were orally administrated with different dosages of Weikangfu recipe, and the growth inhibition was evaluated; apoptotic cells induced were detected by flow cytometry and DNA agarose gel electrophoresis.
  
  
  RESULTS
  Weikangfu recipe showed significant inhibition on the growth of S180 tumor in a dose-dependent manner, compared with the control group. From apoptotic analyses, Weikangfu recipe induced a dose-dependent apoptosis of S180 tumor cells and arrested the cell cycle distribution at G0-G1 phase. At the same time, the up-regulation of p53 and bax and down-regulation of bcl-2 were observed in S180 tumor cells of the treated groups.
  
  
  CONCLUSIONS
  Our findings demonstrate that Weikangfu recipe can significantly inhibit the growth of S180 tumor and induce apoptosis through expression alteration of p53, bax and bcl-2.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China. niexiaohua2000@126.com
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Nie
authors.forename: Bao-Jun
authors.initials: BJ
authors.lastname: Shi
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Tao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D010946:Plants, Medicinal / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011897:Random Allocation; D012510:Sarcoma 180 / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C510020:weikangfu