Selective inhibition of Alu retrotransposition by APOBEC3G.
The non-LTR retrotransposon LINE-1 (L1) comprises approximately 17% of the human genome, and the L1-encoded proteins can function in trans to mediate the retrotransposition of non-autonomous retrotransposons (i.e., Alu and probably SVA elements) and cellular mRNAs to generate processed pseudogenes. Here, we have examined the effect of APOBEC3G and APOBEC3F, cytidine deaminases that inhibit Vif-deficient HIV-1 replication, on Alu retrotransposition and other L1-mediated retrotransposition processes. We demonstrate that APOBEC3G selectively inhibits Alu retrotransposition in an ORF1p-independent manner. An active cytidine deaminase site is not required for the inhibition of Alu retrotransposition and the resultant integration events lack G to A or C to T hypermutation. These data demonstrate a differential restriction of L1 and Alu retrotransposition by APOBEC3G, and suggest that the Alu ribonucleoprotein complex may be targeted by APOBEC3G.
Hulme
Amy E
AE
Department of Human Genetics, The University of Michigan Medical School, Ann Arbor, MI 48109, USA. ahulme@umich.edu
Bogerd
Hal P
HP
Cullen
Bryan R
BR
Moran
John V
JV
D000071480:APOBEC-3G Deaminase; D020087:Alu Elements; D001483:Base Sequence; D003564:Cytidine Deaminase; D043525:Cytosine Deaminase / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000235:genetics; D006801:Humans; D020084:Long Interspersed Nucleotide Elements; D009700:Nucleoside Deaminases / Q000235:genetics / Q000378:metabolism*; D011544:Pseudogenes; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D011994:Recombinant Proteins; D012097:Repressor Proteins; D004247:DNA; D009700:Nucleoside Deaminases; C492906:APOBEC3F protein, human; D043525:Cytosine Deaminase; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase