Fast methods of enantiopurity determination for the Soai reaction: towards a general enantioenrichment detector?
The Soai reaction system possesses a remarkable combination of characteristics (enantioselective autocatalysis, strong positive nonlinearity leading to asymmetric amplification, ability to be triggered by wide variety of exogenous enantioenriched materials) that make it a potential starting point for a generalized detector for enantioenrichment. In this study we apply standard approaches used in pharmaceutical process research to the problem of developing a rapid method for analysis of the enantiopurity of the Soai reaction product. Several methods for rapid analysis (<1 min per sample) are described, including an approach using chiral supercritical fluid chromatography (SFC) and an approach using achiral chromatography with circular dichroism (CD) detection. Some thoughts on incorporation into a device for generalized enantioenrichment detection are presented.
Welch
Christopher J
CJ
Analysis and Preparative Separations Group, Department of Process Research, Merck Research Laboratories, Merck & Co., Inc, NJ 07065, USA. christopher_welch@merck.com
Biba
Mirlinda
M
Sajonz
Peter
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002942:Circular Dichroism; D013237:Stereoisomerism