Plant viral capsids as nanobuilding blocks: construction of arrays on solid supports.
The virions of Cowpea mosaic virus (CPMV) can be regarded as programmable nanobuilding blocks with a diameter of approximately 28 nm. The particles display a number of features that can be exploited for nanoscale material fabrication. In this study we use the virus-derived building blocks for construction of arrays on solid supports. Biotin-modified CPMV particles are used with Streptavidin as a linker molecule in order to enable self-assembly of arrays from the surface up by a layer-by-layer approach. CPMV particles with different fluorescent labels, which enable differential detection of each layer, have been immobilized on surfaces and arranged in defined layers. This approach provides novel structured arrays which have the potential for development as functional devices at the nanoscale.
Steinmetz
Nicole F
NF
Department of Biological Chemistry and Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, United Kingdom.
Calder
Grant
G
Lomonossoff
George P
GP
Evans
David J
DJ
D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D002213:Capsid / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes / Q000494:pharmacology; D006046:Gold / Q000737:chemistry; D008956:Models, Chemical; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry; D049329:Nanostructures; D036103:Nanotechnology / Q000379:methods*; D010942:Plant Viruses / Q000648:ultrastructure; D040081:Protein Array Analysis / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D001710:Biotin; D006046:Gold; D003545:Cysteine