title: [Characteristics of SRAP marker in detecting polymorphism of cucumber genome].
abstract: When SRAP (Sequence-Related Amplified Polymorphism) marker was used in constructing genetic map and analyzing QTL for high temperature resistance in cucumber (Cucumis sativus L.), it exhibited certain characteristics in detecting genomic polymorphism. When each forward primer was combined with different reverse primers, the number of primer combinations that produced polymorphism ranged from five to eight. When each reverse primer was in combination with different forward primers, the number of polymorphic primer combinations ranged from two to eleven. The reverse primers SA4 or EM6 produced identical polymorphic bands when combined with all the forward primers tested. These bands might be amplified by the reverse primers. The polymorphic bands amplified from OD3ME11 co-segregated in the F2 population. The utilization of these characteristics in our research was discussed.
authors.affiliation: College of Life Sciences, Nankai University, Tianjin 300071, China. paozhifeng1@sina.com
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Qian
authors.forename: Gui-Hua
authors.initials: GH
authors.lastname: Zhang
authors.forename: Fei-Xue
authors.initials: FX
authors.lastname: Chen
authors.forename: Yi-Ke
authors.initials: YK
authors.lastname: Han
authors.forename: De-Fu
authors.initials: DF
authors.lastname: Chen
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Du
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: Chen
mesh_terms: D018553:Cucumis sativus / Q000235:genetics* / Q000502:physiology; D017931:DNA Primers; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D006358:Hot Temperature; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci
chemical_list: D017931:DNA Primers; D005819:Genetic Markers