title: Expression, purification, and preliminary X-ray crystallographic analysis of the complex of G(alphai3)-RGS5 from human with GDP/Mg2+)/AlF4-.
abstract: Regulator of G-protein signaling 5 (RGS5), an inhibitor of Gq and Gi activation, is a member of the small RGS protein subfamily. However, despite significant process in the investigation of RGS5, no structure is yet available. In order to elucidate the mechanism of the RGS5 in G protein signaling pathway, we have overexpressed the RGS5 and Galphai(3) from human in Escherichia coli and crystallized the complex of RGS5 and Galphai(3) proteins with GDP/Mg(2+)/AlF(4)(-) at 3.0 A resolution using a synchrotron radiation source. The complex crystals belong to the tetragonal space group P4(1)2(1)2 or P4(3)2(1)2, with unit cell parameters a=b=95.9 A, and c=138.8 A. Assuming one complex protein in the crystallographic asymmetric unit, the calculated Matthews parameter (V(M)) is 2.57 A(3)/Da and solvent content is 52.2 %.
authors.affiliation: Life Sciences Division, Biomedical Research Center, Korea Institute of Science and Technology, Division of Biotechnology, Korea University, Seoul 136-701, South Korea. chahong@korea.ac.kr.
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Rhee
authors.forename: Ki-Hyun
authors.initials: KH
authors.lastname: Nam
authors.forename: Won-Ho
authors.initials: WH
authors.lastname: Lee
authors.forename: Young-Gyu
authors.initials: YG
authors.lastname: Ko
authors.forename: Eunice Eunkyeong
authors.initials: EE
authors.lastname: Kim
authors.forename: Kwang Yeon
authors.initials: KY
authors.lastname: Hwang
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D005459:Fluorides / Q000737:chemistry* / Q000378:metabolism; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008274:Magnesium / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D020710:RGS Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015290:Second Messenger Systems / Q000502:physiology
chemical_list: D017607:Aluminum Compounds; D046912:Multiprotein Complexes; D020710:RGS Proteins; C491349:RGS5 protein, human; D006153:Guanosine Diphosphate; C050992:tetrafluoroaluminate; D019204:GTP-Binding Proteins; C498819:GNAI3 protein, human; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D008274:Magnesium; D005459:Fluorides