title: N-cadherin is a useful marker for the progenitor of cardiomyocytes differentiated from mouse ES cells in serum-free condition.
abstract: Cardiomyocytes are known to differentiate spontaneously from embryonic stem (ES) cells when they formed aggregates, so called "embryoid bodies", in the presence of serum. In this study, we explored the induction of cardiomyocytes from mouse ES cells in chemically defined serum-free medium by using a mesoderm-inducing factor, BMP4. Comparing the different inductive methods, we found a candidate cell surface marker, N-cadherin, for cardiomyocyte progenitors from ES cells. N-cadherin-positive cells highly expressed cardiogenic markers, Nkx2.5, Tbx5, and Isl1, and showed a high differentiation rate into cardiomyocyte lineage. These results indicate that N-cadherin can be a useful cell surface marker for the progenitors of cardiomyocyte differentiated from ES cells in the serum-free culture.
authors.affiliation: Department of Biological Sciences, Graduate School of Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Honda
authors.forename: Akira
authors.initials: A
authors.lastname: Kurisaki
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ohnuma
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Okochi
authors.forename: Tatsuo S
authors.initials: TS
authors.lastname: Hamazaki
authors.forename: Makoto
authors.initials: M
authors.lastname: Asashima
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology; D015820:Cadherins / Q000032:analysis*; D002454:Cell Differentiation; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D051542:Hepatocyte Nuclear Factor 3-beta / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology*; D016133:Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D015820:Cadherins; C496503:Foxa2 protein, mouse; D051542:Hepatocyte Nuclear Factor 3-beta