title: Automation of fluorous solid-phase extraction for parallel synthesis.
abstract: An automatic fluorous solid-phase extraction (F-SPE) technique is developed by using FluoroFlash SPE cartridges on the RapidTrace workstation. A 10-module workstation has the capability to complete a maximum of 100 SPEs each round in 1-2 h. Another important feature of the RapidTrace system is that it has the capability to load slurry samples onto the F-SPE cartridges. The F-SPE cartridge charged with 2 g of fluorous silica gel is used to purify up to 200 mg of crude sample. Sample loading, elution solvent, cartridge reuse, and SPE reproducibility are evaluated. The automatic SPE system is used for purification of a small urea library generated from amine-scavenging reactions using fluorous dichlorotriazine, a 96-membered amide library generated using 2-chloro-4,6-bis[(perfluorohexyl)propyloxy]-1,3,5-triazine as the coupling agent, and another 96-membered library generated from fluorous Mitsunobu reactions. Approximately 90% of the products have > 90% purity after F-SPE.
authors.affiliation: Fluorous Technologies, Inc, University of Pittsburgh Applied Research Center, Pittsburgh, Pennsylvania 15238, USA. w.zhang@fluorous.com
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yimin
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000588:Amines / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D001331:Automation; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004987:Ethers / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry*; D015394:Molecular Structure; D013237:Stereoisomerism; D014508:Urea / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000302:isolation & purification
chemical_list: D000588:Amines; D004987:Ethers; D006845:Hydrocarbons, Fluorinated; D014508:Urea