Kinetics of the immune response to the (F1+V) vaccine in models of bubonic and pneumonic plague.
Protection against aerosol challenge with > 300 MLD of Yersinia pestis was observed 7 days after a single immunisation of mice with the F1+V vaccine. At day 60, mice were protected against injected challenge (10(7)MLD) in a vaccine dose-related manner. Recall responses to rV in splenocytes ex vivo at day 98 correlated significantly (p<0.001) with the immunising dose-level of V antigen; no memory response or anti-V serum IgG was detected in killed whole cell vaccine (KWCV) recipients. This may explain the susceptibility of KWCV recipients to aerosol challenge and the enhanced protection conferred by the F1+V sub-unit vaccine, particularly since the anti-F1 responses induced by either vaccine were similarly IgG1-polarised.
Williamson
E D
ED
Dstl Porton Down, Salisbury, Wilts SP4 0JQ, UK. dewilliamson@dstl.gov.uk
Stagg
A J
AJ
Eley
S M
SM
Taylor
R
R
Green
M
M
Jones
S M
SM
Titball
R W
RW
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010930:Plague / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D010931:Plague Vaccine / Q000276:immunology* / Q000494:pharmacology; D052899:Pore Forming Cytotoxic Proteins / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology / Q000494:pharmacology; D015010:Yersinia pestis / Q000276:immunology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; C043908:LcrV protein, Yersinia; D010931:Plague Vaccine; D052899:Pore Forming Cytotoxic Proteins; D022223:Vaccines, Subunit; C086725:caf1 protein, Yersinia pestis