title: Novel noncoding RNA from human Y distal heterochromatic block (Yq12) generates testis-specific chimeric CDC2L2.
abstract: The human Y chromosome, because it is enriched in repetitive DNA, has been very intractable to genetic and molecular analyses. There is no previous evidence for developmental stage- and testis-specific transcription from the male-specific region of the Y (MSY). Here, we present evidence for the first time for a developmental stage- and testis-specific transcription from MSY distal heterochromatic block. We isolated two novel RNAs, which localize to Yq12 in multiple copies, show testis-specific expression, and lack active X-homologs. Experimental evidence shows that one of the above Yq12 noncoding RNAs (ncRNAs) trans-splices with CDC2L2 mRNA from chromosome 1p36.3 locus to generate a testis-specific chimeric beta sv13 isoform. This 67-nt 5'UTR provided by the Yq12 transcript contains within it a Y box protein-binding CCAAT motif, indicating translational regulation of the beta sv13 isoform in testis. This is also the first report of trans-splicing between a Y chromosomal and an autosomal transcript.
authors.affiliation: Centre for Cellular and Molecular Biology, Uppal Road Hyderabad-500 007, AP, India.
authors.forename: Zeenath
authors.initials: Z
authors.lastname: Jehan
authors.forename: Sambandam
authors.initials: S
authors.lastname: Vallinayagam
authors.forename: Shrish
authors.initials: S
authors.lastname: Tiwari
authors.forename: Suman
authors.initials: S
authors.lastname: Pradhan
authors.forename: Lalji
authors.initials: L
authors.lastname: Singh
authors.forename: Amritha
authors.initials: A
authors.lastname: Suresh
authors.forename: Hemakumar M
authors.initials: HM
authors.lastname: Reddy
authors.forename: Y R
authors.initials: YR
authors.lastname: Ahuja
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Jesudasan
mesh_terms: D000328:Adult; D001483:Base Sequence; D015152:Blotting, Northern; D041322:Chromosomes, Human, Y / Q000235:genetics*; D018844:Cyclin-Dependent Kinases / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006570:Heterochromatin / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D006570:Heterochromatin; D020033:Protein Isoforms; D022661:RNA, Untranslated; C447612:CDK11a protein, human; D018844:Cyclin-Dependent Kinases