title: Dynamics of 17alpha-ethynylestradiol exposure in rainbow trout (Oncorhynchus mykiss): absorption, tissue distribution, and hepatic gene expression pattern.
abstract: 17alpha-Ethynylestradiol (EE2) is a synthetic estrogen identified in sewage effluents. To understand better the absorption kinetics of EE2 and the induction of vitellogenin (VTG) and estrogen receptor alpha (ERalpha) mRNA, we subjected male rainbow trout (Onchorynchus mykiss) to continuous water exposures of 125 ng/L of EE2 for up to 61 d. Trout were either repetitively sampled for blood plasma or serially killed at selected time intervals. Vitellogenin, ERalpha mRNA, and EE2 were measured using enzyme-linked immunosorbent assay and using quantitative polymerase chain reaction and gas chromatography-mass spectrometry, respectively. In separate experiments, trout were exposed to EE2 for 7 d, and hepatic gene expression was assessed using a low- and high-density cDNA microarray. The EE2 was rapidly absorbed by the trout, with an apparent equilibrium at 16 h in plasma and liver. The ERalpha mRNA levels also increased rapidly, reaching near-peak levels by 48 h. In contrast, plasma levels of VTG continuously increased for 19 d. After 61 d, tissues with the highest levels of VTG were the liver, kidney, and testes. Microarray-based gene expression studies provided unexpected results. In some cases, known estrogen-responsive genes (e.g., ERalpha) were unresponsive, whereas many of the genes that have no apparent link to estrogen function or EE2 toxicity were significantly altered in expression. Of the two microarray approaches tested in the present study, the high-density array appeared to be superior because of the improved quality of the hybridization signal and the robustness of the response in terms of the number of genes identified as being EE2 responsive.
authors.affiliation: Pacific Northwest National Laboratory-Marine Sciences Laboratory, 1529 West Sequim Bay Road, Sequim, Washington 98382, USA.
authors.forename: Ann D
authors.initials: AD
authors.lastname: Skillman
authors.forename: James J
authors.initials: JJ
authors.lastname: Nagler
authors.forename: Sharon E
authors.initials: SE
authors.lastname: Hook
authors.forename: Jack A
authors.initials: JA
authors.lastname: Small
authors.forename: Irvin R
authors.initials: IR
authors.lastname: Schultz
mesh_terms: D000818:Animals; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D004997:Ethinyl Estradiol / Q000493:pharmacokinetics* / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014819:Vitellogenins / Q000032:analysis / Q000097:blood
chemical_list: D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D014819:Vitellogenins; D004997:Ethinyl Estradiol