title: [The method of the simultaneous determination of the citrinin and ochratoxin A content in cereals and cereal products].
abstract: The simplifyed method of simultaneous determination of the citrinin (CT) and ochratoxin A (OTA) in cereals is described. The extraction of mycotoxins was carried out by small volumes of solvents (water/aceton/hexane) without additional sample clean up and concentration. The extracts were analysed by HPLC using mixture of methanol/ethylacetate/phosphoric acid, pH 2.2 as a mobile phase and fluorescence detection (lamda ex 330 nm, lamda em 495 nm). The reliability and reproducibility of results were improved by usage of internal standard--methyl-derivative of ochratoxin A. Average recovery of CT and OTA was 70%, relative standard deviation 12% and 7%, respectively, limit of detection 0.003 mg/kg.
authors.forename: K I
authors.initials: KI
authors.lastname: Eller
authors.forename: V V
authors.initials: VV
authors.lastname: Pimenova
authors.forename: M G
authors.initials: MG
authors.lastname: Kiseleva
authors.forename: O I
authors.initials: OI
authors.lastname: Perederiaev
authors.forename: I V
authors.initials: IV
authors.lastname: Aksenov
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Medvedev
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002953:Citrinin / Q000032:analysis*; D002523:Edible Grain / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A; D002953:Citrinin