Toward hybridization assays without PCR using universal nanoamplicons.
An innovative scheme for signal amplification using random tetramer-modified gold nanoparticles, termed "nanoamplicons," has been developed for hybridization assay without PCR. Large numbers of nanoamplicons could be integrated onto one target, providing much greater amplification than the larger nanoparticles usually adopted. Using M13mp18 single-strand DNA as a target, this concept is shown to be a feasible approach to detecting 0.17 amol L(-1) DNA without target amplification, based on microgravimetric detection of the adsorption of the probe-target-nanoamplicons complex via thiol-gold binding. To our knowledge, this method has a sensitivity that is close to that of PCR and superior to those of nanoparticle-based methods reported previously. Additionally, this novel nanoamplicon could be prepared in the same way and used for all diagnostic tests; such universality would make the nanoamplicons highly advantageous for the generalization and standardization of bioassays, and when applying this new technology in clinical laboratories.
Mo
Z-H
ZH
Micro-System Research Center, College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, People's Republic of China. zhihmo@cqu.edu.cn
Wei
X-L
XL
D004247:DNA / Q000032:analysis* / Q000235:genetics; D053758:Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction
D004247:DNA