[CD34(+)/CD123(+) cell sorting from the patients with leukemia by Midi MACS method].
The aim of this study was to sort the CD34(+)/CD123(+) cells from the bone marrow cells of patients with acute myeloid leukemia (AML) by Midi MACS method. Firstly, the bone marrow mononuclear cells (BMMNC) were isolated from the patients with AML with Ficoll Paque, CD34(+) cells were then isolated by Midi MACS method followed by the isolation of CD34(+)/CD123(+) cells from the fraction of CD34(+) cells. The enrichment and recovery of CD34(+) and CD34(+)/CD123(+) cells were assayed by FACS technique. The results showed that the enrichment of CD34(+) cells was up to 98.73%, its average enrichment was 95.6%, and the recovery of CD34(+) was 84.6%, its average recovery was 51% after the first round sorting, by the second round sorting, the enrichment of CD34(+)/CD123(+) cells was up to 99.23%, its average enrichment was 83%. With regard to BMMNCs before sorting, the recovery of CD34(+)/CD123(+) was 34%. But, on the CD34(+) cells obtained by the first round sorting, its recovery was 56%. In conclusion, these results confirmed that the method of Midi MACS sorting can be applied to sort CD34(+)/CD123(+) cells from the bone marrow cells of AML patients, which give rise to the similar enrichment and recovery of the sorted cells with that of literature reported by the method of FACS.
Wang
Guang-Ping
GP
Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China. guangpingw@yahoo.com
Cao
Xin-Yu
XY
Xin
Hong-Ya
HY
Li
Qun
Q
Qi
Zhen-Hua
ZH
Chen
Fang-Ping
FP
D018952:Antigens, CD34 / Q000032:analysis*; D001854:Bone Marrow Cells / Q000473:pathology*; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D053650:Interleukin-3 Receptor alpha Subunit / Q000032:analysis*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000473:pathology
D018952:Antigens, CD34; D053650:Interleukin-3 Receptor alpha Subunit