Infusion of hypertonic saline (7.5%) does not change neutrophil oxidative burst or expression of endothelial adhesion molecules after abdominal hysterectomy.
BACKGROUND
Previous studies found hypertonicity to affect neutrophils in intact laboratory animals and in human blood cell cultures. We investigated whether infusion of hypertonic saline in a clinical relevant dose before hysterectomy affected peripheral blood neutrophils and their response to surgery.


METHODS
Fifteen women scheduled for open abdominal hysterectomy were randomized double-blindly to infusion of 4 mL/kg 7.5% NaCl, 4 mL/kg 0.9% NaCl, or 32 mL/kg 0.9% NaCl over 20 minutes. Blood was collected at baseline, after infusion, 1, 4, and 24 hours postoperatively for the determination of leukocyte and differential count, neutrophil membrane expression of endothelial adhesion molecules by flow cytometry, and O2- -generation by superoxide dismutase-inhibitable reduction of cytochrome C.


RESULTS
Surgery induced well-known changes in the number and distribution of white blood cells, reduced the expression of adhesion molecules, and halved the superoxide production unrelated to the tonicity or volume of the infused fluids.


CONCLUSIONS
Infusion of a clinically relevant dose of hypertonic saline has no detectable effect on the membrane expression of endothelial adhesion molecules or O2- -generation in circulating neutrophils after elective abdominal hysterectomy.
Kolsen-Petersen
Jens Aage
JA
Department of Anesthesia and Intensive Care, Viborg County Hospital, Viborg, DK. jaakp@dadlnet.dk
Rasmussen
Torsten Bøgh
TB
Krog
Jan
J
Hokland
Marianne
M
Tonnesen
Else Kirstine
EK
D000328:Adult; D000704:Analysis of Variance; D015703:Antigens, CD / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004311:Double-Blind Method; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007044:Hysterectomy / Q000379:methods; D016023:Integrins / Q000096:biosynthesis*; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D009994:Osmolar Concentration; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage*; D019087:Selectins / Q000378:metabolism*; D012964:Sodium / Q000097:blood; D013481:Superoxides / Q000378:metabolism*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D015703:Antigens, CD; D006454:Hemoglobins; D016023:Integrins; D012462:Saline Solution, Hypertonic; D019087:Selectins; D019010:Vascular Cell Adhesion Molecule-1; D013481:Superoxides; D012964:Sodium