title: Synthesis, characterization, and utility of thermoresponsive natural/unnatural product macroligands for affinity chromatography.
abstract: [Structure: see text] The synthesis and characterization of thermoresponsive, water-soluble poly-N-isopropyl acrylamide (PNIPAM) derived macroligands displaying cyclosporin A (CsA) and dexamethasone (Dex) for use as novel affinity resins are described. Characterization of these soluble macroligands, including ligand loading and integrity, was determined by 1H NMR spectroscopy. One of the CsA macroligands was used in a protein affinity experiment to capture known binding proteins of CsA, the cyclophilins, from Jurkat T-cell lysates.
authors.affiliation: Department of Chemistry, Texas A&M University, P.O. Box 30012, College Station, Texas 77842-3012, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.forename: Ananthapadmanab
authors.initials: A
authors.lastname: Sivaramakrishnan
authors.forename: Krishan
authors.initials: K
authors.lastname: Ponnamperuma
authors.forename: Woon-Kai
authors.initials: WK
authors.lastname: Low
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.forename: Jun O
authors.initials: JO
authors.lastname: Liu
authors.forename: David E
authors.initials: DE
authors.lastname: Bergbreiter
authors.forename: Daniel
authors.initials: D
authors.lastname: Romo
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D021983:Cyclophilins; D016572:Cyclosporine / Q000737:chemistry*; D003907:Dexamethasone / Q000737:chemistry*; D005938:Glucocorticoids / Q000737:chemistry; D006801:Humans; D007166:Immunosuppressive Agents / Q000737:chemistry; D019169:Jurkat Cells; D008024:Ligands; D015394:Molecular Structure; D011485:Protein Binding
chemical_list: D005938:Glucocorticoids; D007166:Immunosuppressive Agents; D008024:Ligands; D003907:Dexamethasone; D016572:Cyclosporine; D021983:Cyclophilins