[Synergistic effects of human papillomavirus and phorbol ester in cell transformation.].
BACKGROUND
To investigate the molecular mechanism of the synergistic effects of human papillomavirus (HPV) and phorbal esters (TPA) in cell transformation.


METHODS
The expression of oncogenes and anti-oncogenes in 293 cell line treated with HPV and TPA was studied by Southern Blot and RNA dot blot.


RESULTS
It was found that the synergistic effect induced the amplification of c-myc (4-8 times), increased expressing level of c-erbB-2 (32-64 times) and decreased expressing level of p16(1/4-1/8).


CONCLUSIONS
The above results show that the synergistic effect has an important role in development of carcinoma.
Cen
S
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Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Zhang
Y
Y
Xu
J J
JJ
Shen
Z Y
ZY
Zeng
Y
Y
D002460:Cell Line; D006801:Humans; D009857:Oncogenes; D027383:Papillomaviridae / Q000235:genetics; D010703:Phorbol Esters; D012333:RNA, Messenger / Q000235:genetics
D010703:Phorbol Esters; D012333:RNA, Messenger