[Preparation of antibody against Memo protein and analysis of expression profile of Memo in mouse tissues].
OBJECTIVE
To prepare and characterize antibody against Memo protein and to detect the tissue distribution of Memo in mice.


METHODS
Fusion protein GST-Memo was expressed and purified, and polyclonal antibody against Memo was prepared by immunizing mice. A FLAG-tagged eukaryotic expression vector pcDNA3-FLAG-Memo was constructed. The specificity of the antibody was detected by Western blot.


RESULTS
An eukaryotic expression vector pcDNA3-FLAG-Memo was obtained. The polyclonal antibody was found to be specific to Memo. Memo protein was widely expressed in mouse tissues using the obtained antibody in Western blot.


CONCLUSIONS
Antibody specific to Memo has been successfully obtained, which provides useful tool for investigation into Memo-associated mechanisms of tumor metastasis and invasiveness.
Ning
Kang
K
Institute of Bioengineering, Academy of Military Medical Sciences, Beijing 100850, China. ningkangnk@163.com
Zhang
Hao
H
Han
Ju-Qiang
JQ
Fan
Hong-Xue
HX
Li
Jie-Zhi
JZ
Li
Rong
R
Ye
Qi-Nong
QN
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D051379:Mice; D019159:Nonheme Iron Proteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*; D009928:Organ Specificity; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism
D000906:Antibodies; C540429:Memo protein, mouse; D019159:Nonheme Iron Proteins; D011993:Recombinant Fusion Proteins