title: [Construction and preliminary application of a human natural phage antibody library with diversity].
abstract: OBJECTIVE
  To construct a human natural phage single-chain antibody (scFv) library with diversity.
  
  
  METHODS
  V(H) and V(L) genes were amplified by RT-PCR and hemi-PCR from peripheral blood lymphocytes of healthy persons. The V genes were assembled to form scFv by overlap PCR and cloned into phagemid pCANTAB-5E, and then transformed into E. coli TG1 by electroporation to construct a human natural phage scFv library. The diversity and gene family of antibody gene were analysed by sequencing and the specific antibodies against various antigens were screened through bio-panning.
  
  
  RESULTS
  A human natural phage scFv library with diversity and 2x10(8) sink size was constructed successfully. The specific human scFvs against 5 antigens were obtained by bio-panning.
  
  
  CONCLUSIONS
  A human natural phage scFv library with diversity is constructed successfully and can be applied to human antibody preparation.
authors.affiliation: The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Research Center for Medical Molecular Virology of Fujian Province, Xiamen 361005, China. wxluo@jingxian.xmu.edu.cn
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Wen-Xin
authors.initials: WX
authors.lastname: Luo
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Li
authors.forename: Ying-Wei
authors.initials: YW
authors.lastname: Chen
authors.forename: Ming-Qiao
authors.initials: MQ
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Ning-Shao
authors.initials: NS
authors.lastname: Xia
mesh_terms: D000906:Antibodies / Q000032:analysis / Q000235:genetics* / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000032:analysis / Q000235:genetics / Q000276:immunology; D019151:Peptide Library; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D000906:Antibodies; D000941:Antigens; D007135:Immunoglobulin Variable Region; D019151:Peptide Library