title: Time-lapse analysis of stem-cell divisions in the Arabidopsis thaliana root meristem.
abstract: In the Arabidopsis root, asymmetric stem-cell divisions produce daughters that form the different root cell types. Here we report the establishment of a confocal tracking system that allows the analysis of numbers and orientations of cell divisions in root stem cells. The system provides direct evidence that stem cells have lower division rates than cells in the proximal meristem. It also allows tracking of cell division timing, which we have used to analyse the synchronization of root cap divisions. Finally, it gives new insights into lateral root cap formation: epidermal stem-cell daughters can rotate the orientation of the division plane like the stem cell.
authors.affiliation: Department of Molecular Genetics, University of Utrecht, Utrecht, the Netherlands.
authors.forename: Ana
authors.initials: A
authors.lastname: Campilho
authors.forename: Bernardo
authors.initials: B
authors.lastname: Garcia
authors.forename: Henk V D
authors.initials: HV
authors.lastname: Toorn
authors.forename: Henk V
authors.initials: HV
authors.lastname: Wijk
authors.forename: Aurélio
authors.initials: A
authors.lastname: Campilho
authors.forename: Ben
authors.initials: B
authors.lastname: Scheres
mesh_terms: D017360:Arabidopsis / Q000166:cytology*; D002455:Cell Division; D018519:Meristem / Q000166:cytology*; D017440:Photoperiod; D018517:Plant Roots / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors