title: Quantitative expression of apoptosis-regulating genes in endometrium from women with and without endometriosis.
abstract: OBJECTIVE
  To quantitate antiapoptotic and proapoptotic gene expression in endometrial cells (ECs) of women with and without endometriosis.
  
  
  METHODS
  Determination of transcript abundance (TA) of apoptosis-regulating genes in eutopic and ectopic endometrial cells.
  
  
  METHODS
  Institute for the Study and Treatment of Endometriosis, Chicago, Illinois, and university-based research laboratories.
  
  
  METHODS
  Women with (n = 10) and without (n = 6) endometriosis.
  
  
  METHODS
  None.
  
  
  METHODS
  Quantitative virtually multiplexed transcript abundance measurement (VMTA) of the BCL2, BCLxL, defender against cell death-1 (DAD-1), BCLxS, P53, Caspase-1, and proliferating cell nuclear antigen (PCNA) genes.
  
  
  RESULTS
  The TA ratio of antiapoptotic to proapoptotic isoforms of the BCL-X gene favors survival in eutopic and ectopic ECs from women with endometriosis, but not control ECs. This was found throughout the menstrual cycle for ectopic ECs. Eutopic but not ectopic ECs also expressed increased TA of the antiapoptotic DAD-1 gene in endometriosis. Eutopic and ectopic ECs from women with endometriosis expressed decreased TA of p53 and Caspase-1 compared to ECs from women without endometriosis. Expression of these genes was not correlated with the proliferative state of ECs based on TA of the PCNA gene.
  
  
  CONCLUSIONS
  Dysregulation in expression of pro- and antiapoptotic regulatory genes characterizes eutopic and ectopic ECs from women with endometriosis. These results are consistent with apoptotic resistance and enhanced survival of ECs in endometriosis.
authors.affiliation: Cancer Institute, Departments of Surgery and Medicine, Medical University of Toledo, Toledo, Ohio 43614-5809, USA. donald.braun@ctca-hope.com
authors.forename: Donald P
authors.initials: DP
authors.lastname: Braun
authors.forename: Jianchi
authors.initials: J
authors.lastname: Ding
authors.forename: Fehr
authors.initials: F
authors.lastname: Shaheen
authors.forename: James C
authors.initials: JC
authors.lastname: Willey
authors.forename: Nasir
authors.initials: N
authors.lastname: Rana
authors.forename: W Paul
authors.initials: WP
authors.lastname: Dmowski
mesh_terms: D000328:Adult; D051017:Apoptosis Regulatory Proteins / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans
chemical_list: D051017:Apoptosis Regulatory Proteins; D015415:Biomarkers