title: [Mechanism of acute liver injury induced by crushing hindlimbs in rabbits].
abstract: OBJECTIVE
  To investigate the role of oxidative stress in acute liver injury during crushing hindlimbs in rabbit.
  
  
  METHODS
  The crushing injury model in rabbit was established by intermittent crushing the hind limbs of rabbit with standard weight. The ALT and AST activities were spectrophotometrically measured. The weight ratio (wet/dry,W/D) of livers was measured with scale, and the pathologic changes were observed. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total anti-oxidant capacity (T-AOC) as well as malondialdehyde (MDA) level were spectrophotometrically measured.
  
  
  RESULTS
  As compared with control rabbits, crushing hindlimbs of rabbits induced acute liver injury with the increase in ALT and AST activities in serum,which were 4.31 (P < 0.01) and 10.54 times (P < 0.01) of control group respectively, there were cellular swellings and slight congestion of hepatic sinuses. In addition,crushing hind-limbs elicited significant decrease in SOD,CAT,GSH-Px activity and T-AOC to 17%, 29%, 24% and 21% (P < 0.01) compared with control group respectively, whereas MDA level markedly enhanced.
  
  
  CONCLUSIONS
  Crushing hindlimbs of rabbits induced acute liver injury and significant decrease in anti-oxidant capacity, the latter maybe play an important role in crushing hind-limbs of rabbits-elicited the acute liver injury.
authors.affiliation: Department of Forensic Medicine, Medical School of Soochow University, Suzhou 215123, China.
authors.forename: Ye-Xing
authors.initials: YX
authors.lastname: Han
authors.forename: Zhen-Yong
authors.initials: ZY
authors.lastname: Gu
authors.forename: Bin
authors.initials: B
authors.lastname: Cong
authors.forename: Xin-Hai
authors.initials: XH
authors.lastname: Cen
authors.forename: Dong-Xu
authors.initials: DX
authors.lastname: Liu
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
mesh_terms: D000208:Acute Disease; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006614:Hindlimb / Q000293:injuries*; D008099:Liver / Q000293:injuries / Q000378:metabolism / Q000473:pathology*; D008107:Liver Diseases / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D011817:Rabbits; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase