A novel zinc-finger protein ZNF436 suppresses transcriptional activities of AP-1 and SRE.
Mitogen-activated protein kinases (MAPKs) are evolutionary conserved enzymes in cell signal transduction connecting cell-surface receptors to critical regulatory targets within cells and control cell survival, adaptation, and proliferation. Previous studies revealed that zinc finger proteins are involved in the regulation of the MAPK signaling pathways. Here we report the identification and characterization of a novel human zinc finger protein, ZNF436. The cDNA of ZNF436 is 3.8 kb, encoding 470 amino acids in the nucleus. The protein is highly conserved in evolution across different vertebrate species from rat to human. RT-PCR indicates that ZNF436 is expressed in all the human fetal tissues examined, with a high level in brain and heart. Overexpression of pCMV-tag2A-ZNF436 in the COS-7 cells represses the transcriptional activities of SRE and AP-1. These results suggest that ZNF436 is a member of the zinc finger transcription factor family and may act as a negative regulator in gene transcription mediated by the MAPK signaling pathways.
Li
Yongqing
Y
The Center for Heart Development, College of Life Sciences, Hunan Normal University, 410081, Changsha, Hunan, People's Republic of China.
Du
Xiaoyan
X
Li
Fang
F
Deng
Yun
Y
Yang
Zhiyuan
Z
Wang
Yuequn
Y
Pen
Zhonglu
Z
Wang
Zequn
Z
Yuan
Wuzhou
W
Zhu
Chuanbing
C
Wu
Xiushan
X
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015536:Down-Regulation; D019143:Evolution, Molecular; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006321:Heart / Q000196:embryology; D006801:Humans; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D010802:Phylogeny; D017385:Sequence Homology; D014018:Tissue Distribution; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; C502774:SRE protein, human; D018808:Transcription Factor AP-1; D014157:Transcription Factors; C522122:ZNF436 protein, human; D049452:Green Fluorescent Proteins