title: Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment.
abstract: This study defines the in vitro phenomenon of ciliated bovine bronchial epithelial cell (BBEC) detachment from the basal epithelium and regulation of cilia motility mediated through protein kinase C epsilon (PKCepsilon). The authors determined the time course of activation and downregulation of PKCepsilon by the known PKC activator phorbol 12-myristate 13-acetate (PMA) and demonstrate that chemical inhibition of PKC by calphostin C or the novel PKC isoform inhibitor Ro 31-8220 induced striking detachment of ciliated BBECs from the basal cell monolayer within 1 hour, independent of apoptosis or necrotic cell death. The results of this study support a possible novel PKCepsilon-mediated signaling pathway through which ciliated cell attachment is maintained.
authors.affiliation: Pulmonary, Critical Care and Sleep Medicine Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198-5300, USA.
authors.forename: Rebecca E
authors.initials: RE
authors.lastname: Slager
authors.forename: Joseph H
authors.initials: JH
authors.lastname: Sisson
authors.forename: Jaqueline A
authors.initials: JA
authors.lastname: Pavlik
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Johnson
authors.forename: Jeremy R
authors.initials: JR
authors.lastname: Nicolarsen
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Jerrells
authors.forename: Todd A
authors.initials: TA
authors.lastname: Wyatt
mesh_terms: D000818:Animals; D001980:Bronchi / Q000166:cytology*; D002273:Carcinogens / Q000494:pharmacology; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002923:Cilia / Q000187:drug effects / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D009281:Naphthalenes / Q000494:pharmacology; D051744:Protein Kinase C-epsilon / Q000037:antagonists & inhibitors* / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology* / Q000201:enzymology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D002273:Carcinogens; D004791:Enzyme Inhibitors; D007211:Indoles; D009281:Naphthalenes; C061465:calphostin complex; D051744:Protein Kinase C-epsilon; D013755:Tetradecanoylphorbol Acetate; C064758:Ro 31-8220