Expression and effect of transforming growth factor-alpha and tumor necrosis factor-alpha in human pheochromocytoma.
This study observed the expression of transforming growth factor-alpha (TGF-alpha) and tumor necrosis factor-alpha (TNF-alpha) in pheochromocytoma (PHEO) tissue and examined their effects on the proliferation and apoptosis of human PHEO cells. The mRNA and protein expressions of TGF-alpha and TNF-alpha were higher in PHEO tissues than in normal adrenal medullary tissues, and their expressions varied with pathological features. TGF-alpha and TNF-alpha stimulated the proliferation of primary human PHEO cells, but had no effect on the cell apoptosis. Both TGF-alpha and TNF-alpha might be involved in the pathogenesis of human PHEO. TNF-alpha needs to be further investigated before its treatment of PHEO can be realized in clinical practice.
Tong
An-Li
AL
Department of Endocrinology, Peking Union Medical College Hospital, 100730 Beijing, China. zengzhengpei@yahoo.com.cn
Zeng
Zheng-Pei
ZP
Li
Han-Zhong
HZ
Yang
Di
D
Lu
Lin
L
Li
Ming
M
D000310:Adrenal Gland Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017209:Apoptosis; D049109:Cell Proliferation; D006801:Humans; D007150:Immunohistochemistry; D010673:Pheochromocytoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016211:Transforming Growth Factor alpha / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D016211:Transforming Growth Factor alpha; D014409:Tumor Necrosis Factor-alpha