[Studies on in vitro culture of adventitious root in Salvia miltiorrhiza].
OBJECTIVE
To study the culture of adventitious root of Salvia miltiorrhiza in vitro systemically.


METHODS
Effects of sucrose concentrations, medium pH, inoculum size and plant growth regulators on adventitious root growth and secondary metabolites production in S. miltiorrhiza were investigated.


RESULTS
With the increase of initial sucrose concentration, adventitious root growth rates increased and tanshinone II A content decreased, while content of protocatechuic aldehyde showed a broken line change and its highest production was obtained under 30 g x L(-1) sucrose in the medium. As for the effect of medium pH, medium pH of 6.5, 5.5 (or 6.0) and 5.8 was favorable for adventitious root growth, tanshinone II A and protocatechuic aldehyde synthesis respectively. Furthermore, adventitious root growth, rate was greatly increased when inoculum size was 2.5%. MS medium added with 0.5 mg x L(-1) KT was much favorable for tanshinone II A and protocatechuic aldehyde accumulation.


CONCLUSIONS
Parameters including sucrose concentrations, medium pH, inoculum size and plant growth regulators have distinct effects on the in vitro culture of adventitious root growth and secondary metabolites synthesis of S. miltiorrhiza.
Chen
Wei
W
The College of Pharmaceuticals Science and Technology, Tianjin University, Tianjin 300072, China.
Guo
Xiao-Hong
XH
Gao
Wen-Yuan
WY
Chen
Hai-Xia
HX
Huang
Lu-Qi
LQ
Xiao
Pei-Gen
PG
D045784:Abietanes; D001547:Benzaldehydes / Q000378:metabolism; D002396:Catechols / Q000378:metabolism; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D010616:Phenanthrenes / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000254:growth & development* / Q000378:metabolism; D013395:Sucrose; D046509:Tissue Culture Techniques
D045784:Abietanes; D001547:Benzaldehydes; D002396:Catechols; D003470:Culture Media; D010616:Phenanthrenes; D010937:Plant Growth Regulators; C021751:tanshinone; C005581:protocatechualdehyde; D013395:Sucrose