title: Purification by silica gel chromatography using dialysis tubing and characterization of sophorolipids produced from Candida bombicola grown on glucose and arachidonic acid.
abstract: The yeast Candida bombicola (ATCC 22214) grown on primary carbon source glucose (100 g l(-1)) and secondary carbon, arachidonic acid (2 g l(-1)) produced mixture of sophorolipids up to 1.44 g l(-1). The crude product was a heterogeneous mixture of sophorolipids, which are glycolipids of sophorose linked to the fatty acid through glycosidic bond between omega and omega-1 carbon of arachidonic acid. The derived sophorolipids were isolated by silica gel chromatography using dialysis tubing. The purified sophorolipids were characterized by ESI-MS and FT-IR. Acid hydrolysis of the resolved sophorolipids were characterized by ESI-MS for the presence of 20-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid (20-HETE) and 19-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid (19-HETE), compounds of pronounced pharmaceutical importance.
authors.affiliation: Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
authors.forename: Sachin
authors.initials: S
authors.lastname: Shah
authors.forename: Asmita
authors.initials: A
authors.lastname: Prabhune
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism*; D002175:Candida / Q000378:metabolism*; D002850:Chromatography, Gel / Q000295:instrumentation / Q000379:methods*; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D005936:Glucans / Q000737:chemistry; D005947:Glucose / Q000378:metabolism*; D006017:Glycolipids / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D015394:Molecular Structure; D012822:Silicon Dioxide / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005936:Glucans; D006017:Glycolipids; D016718:Arachidonic Acid; D012822:Silicon Dioxide; D005947:Glucose; C003328:sophorose