Identification and characterization of a fructose phosphotransferase system in Bifidobacterium breve UCC2003.
In silico analysis of the Bifidobacterium breve UCC2003 genome allowed identification of four genetic loci, each of which specifies a putative enzyme II (EII) protein of a phosphoenolpyruvate:sugar phosphotransferase system. The EII encoded by fruA, a clear homologue of the unique EIIBCA enzyme encoded by the Bifidobacterium longum NCC2705 genome, was studied in more detail. The fruA gene is part of an operon which contains fruT, which is predicted to encode a homologue of the Bacillus subtilis antiterminator LicT. Transcriptional analysis showed that the fru operon is induced by fructose. The genetic structure, complementation studies, and the observed transcription pattern of the fru operon suggest that the EII encoded in B. breve is involved in fructose transport and that its expression is controlled by an antiterminator mechanism. Biochemical studies unequivocally demonstrated that FruA phosphorylates fructose at the C-6 position.
Mazé
Alain
A
Department of Microbiology and Alimentary Pharmabiotic Centre, University of Ireland, Cork, Western Road, Cork, Ireland.
O'Connell-Motherway
Mary
M
Fitzgerald
Gerald F
GF
Deutscher
Josef
J
van Sinderen
Douwe
D
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001644:Bifidobacterium / Q000201:enzymology* / Q000235:genetics; D005632:Fructose / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D005632:Fructose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System