Protein-bound dinitrosyl-iron complexes appearing in blood of rabbit added with a low-molecular dinitrosyl-iron complex: EPR studies.
The formation of protein-bound dinitrosyl-iron complexes (DNIC) in blood plasma and packed red cell fraction has been demonstrated by the EPR method in the experiments on rabbits which were i/v injected with the low-molecular DNIC with thiosulphate. This formation was ensured by transfer of Fe(+)(NO(+))(2) moieties from low-molecular DNIC onto serum albumin or hemoglobin molecules. Protein-bound DNICs appeared immediately after low-molecular DNIC injection followed with gradually decreasing their amounts. The complexes could be detected by EPR technique during more than two days. The addition of water-soluble NO scavenger, the iron complex with N-methyl-d-glucamine dithiocarbamate (MGD) resulted in decomposition of a part of protein-bound DNICs and in effective excretion of secondary products (mainly mononitrosyl-iron complexes with MGD) from the blood flow.
Timoshin
Alexander A
AA
Russian Cardiologic Research-and-Production Complex, Russian Ministry of Health, Moscow, Russia.
Vanin
Anatoly F
AF
Orlova
Tsvetina R
TR
Sanina
Natalia A
NA
Ruuge
Enno K
EK
Aldoshin
Sergey M
SM
Chazov
Evgeny I
EI
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D007501:Iron / Q000097:blood* / Q000737:chemistry; D009589:Nitrogen Oxides / Q000097:blood* / Q000737:chemistry; D011817:Rabbits
D001798:Blood Proteins; D009589:Nitrogen Oxides; C073661:dinitrosyl iron complex; D007501:Iron