[Study on antitumor activities of huanglian jiedu decoction].
OBJECTIVE
To study the antitumor activity of Huanglian Jiedu decoction (HLJDT).


METHODS
Antitumor activities were tested in mice with experimental tumor H22 in vivo, and the thymus index, spleen index and tumor inhibitory rate were evaluated. The effects on cancer cells from human were investigated in vitro using serum pharmacological approach. Swille, SPC-A-1, SGC-7901 and MCF-7 cancer cells were incubated in culture media containing serum from mice medicated with HLJDT. The inhibitory effects of HLJDT serum were observed by MTT assay.


RESULTS
HLJDT showed significant antitumor activities on H22 in mice. All of the HLJDT serum in different dosage groups could highly inhibit the proliferation of 4 cancer cell lines from human.


CONCLUSIONS
The HLJDT can significantly inhibit the tumor H22 in mice in a dose-dependent manner, the drug serum has obvious anticancer effects against Swille, SPC-A-1, SGC-7901 and MCF-7.
Sun
Jian
J
School of Pharmacy, Jilin University, Changchun 130021, China. sunjiankw@163.com
Wen
Qing-Hui
QH
Song
Yu
Y
Li
Xia
X
Jin
Jin
J
Ma
Ji-Sheng
JS
Zhou
Qiu-Li
QL
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D013950:Thymus Gland / Q000473:pathology
D000972:Antineoplastic Agents, Phytogenic; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal