title: Individually resolved DNA molecules stretched and embedded in electrospun polymer nanofibers.
abstract: We have used the flow characteristics of an electrospinning jet to elongate and fix DNA molecules. We embedded and observed fluorescently labeled lambda bacteriophage DNA molecules in polyethylene oxide nanofibers. The embedded DNA molecules were imaged using fluorescence microscopy and found to be stretched to lengths approaching the full dyed contour length.
authors.affiliation: School of Applied and Engineering Physics and Department of Physics, Cornell University, Ithaca, New York 14853, USA. Lmb79@cornell.edu
authors.forename: Leon M
authors.initials: LM
authors.lastname: Bellan
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Cross
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Strychalski
authors.forename: Jose
authors.initials: J
authors.lastname: Moran-Mirabal
authors.forename: H G
authors.initials: HG
authors.lastname: Craighead
mesh_terms: D010582:Bacteriophage lambda / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods; D043942:Nanotubes / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D011092:Polyethylene Glycols; D004247:DNA