title: Protein dolichylation in Plasmodium falciparum.
abstract: We performed reverse-phase thin-layer chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC) analysis of polyisoprenoids released by sulfonium-salt cleavage with methyl iodide from Plasmodium falciparum proteins labeled with [3H]FPP or [3H]GGPP and showed that a dolichol of 11 isoprene units is bound to 21-28-kDa protein clusters from trophozoite and schizont stages. The dolichol structure was confirmed by electrospray-ionization mass spectrometry analysis. Treatment with protein synthesis inhibitors and RP-HPLC analysis of the proteolytic digestion products from parasite proteins labeled with [35S]cysteine and [3H]FPP showed that the attachment of dolichol to protein is a post-translational event and probably occurs via a covalent bond to cysteine residues.
authors.affiliation: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Fabio Luiz
authors.initials: FL
authors.lastname: D'Alexandri
authors.forename: Emilia Akemi
authors.initials: EA
authors.lastname: Kimura
authors.forename: Valnice Jesus
authors.initials: VJ
authors.lastname: Peres
authors.forename: Alejandro Miguel
authors.initials: AM
authors.lastname: Katzin
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D004286:Dolichols / Q000737:chemistry / Q000378:metabolism*; D010963:Plasmodium falciparum / Q000737:chemistry / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D004286:Dolichols; D011500:Protein Synthesis Inhibitors; D015800:Protozoan Proteins; D003545:Cysteine