title: Purification and assay of the human INO80 and SRCAP chromatin remodeling complexes.
abstract: Chromatin modifying and remodeling enzymes play critical roles in many aspects of chromosome biology including transcription, replication, recombination, and repair. Our laboratory recently identified and characterized two multisubunit human chromatin remodeling enzymes designated the INO80 and SRCAP complexes. Mechanistic studies revealed that the human INO80 complex catalyzes nucleosome sliding and the SRCAP complex catalyzes ATP-dependent exchange of histone H2A/H2B dimers containing the histone variant H2A.Z into nucleosomes. Here we describe methods for purification and assay of the INO80 and SRCAP chromatin remodeling complexes.
authors.affiliation: Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cai
authors.forename: Jingji
authors.initials: J
authors.lastname: Jin
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Gottschalk
authors.forename: Tingting
authors.initials: T
authors.lastname: Yao
authors.forename: Joan W
authors.initials: JW
authors.lastname: Conaway
authors.forename: Ronald C
authors.initials: RC
authors.lastname: Conaway
mesh_terms: D000251:Adenosine Triphosphatases / Q000302:isolation & purification*; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D002846:Chromatography, Affinity / Q000379:methods*; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D006801:Humans; D046912:Multiprotein Complexes / Q000302:isolation & purification*; D009707:Nucleosomes / Q000378:metabolism; D014157:Transcription Factors / Q000302:isolation & purification*; D000092468:Bromodomain Containing Proteins
chemical_list: D000251:Adenosine Triphosphatases; D002868:Chromosomal Proteins, Non-Histone; D046912:Multiprotein Complexes; D009707:Nucleosomes; D014157:Transcription Factors; C492319:BAZ1A protein, human; D000092468:Bromodomain Containing Proteins; C119652:SRCAP protein, human