[Content comparison of pinoresinol diglucoside in original and reborn bark of Eucommia ulmoides].
OBJECTIVE
To evaluate the reborn barks quality, antihypertensive effective component of pinoresnol diglucoside (PDG) in three times reborn barks were determined.


METHODS
A YWG C18 column (10 microm, 250 x 4.6 mm) was used with a mobile phase of methanol-water (30:75) and flow rate of 1.0 (ml/min). The detective wave-length was set at 277nm and the column temperature at room temperature.


RESULTS
PDG in the first reborn barks are slightly higher than the original ones, and in the second reborn barks are similar with the barks before girdling (the fist reborn barks), but in the third reborn barks, PDG are much lower than the barks before girdling (the second reborn barks).


CONCLUSIONS
In order to ensure reborn barks quality, we suggest that the girdling bark regeneration can be made two times, the time between the first and the second girdling is not less than five years. PDG in the third reborn barks should be enhanced.
Feng
Suo-Min
SM
Biology and Medicine Key Laboratory of Northwest University, Xi'an 710069, China.
Gan
Zhi-Jie
ZJ
Zhai
Xi-Feng
XF
Fu
Peng-Fei
PF
Sun
Wen-Ji
WJ
D002851:Chromatography, High Pressure Liquid; D003247:Conservation of Natural Resources; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D031283:Eucommiaceae / Q000737:chemistry* / Q000378:metabolism; D017705:Lignans / Q000096:biosynthesis / Q000302:isolation & purification*; D024301:Plant Bark / Q000033:anatomy & histology / Q000737:chemistry / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry*; D012038:Regeneration; D013997:Time Factors; D014465:Ultrasonics
D004365:Drugs, Chinese Herbal; D017705:Lignans; C013200:pinoresinol diglucoside