title: Functionality of NGF-protected PC12 cells following exposure to 6-hydroxydopamine.
abstract: 6-Hydroxydopamine (6-OHDA) is often used in models of Parkinson's disease since it can selectively target and kill dopaminergic cells of the substantia nigra. In this study, pre-treatment of PC12 cells with nerve growth factor (NGF) inhibited apoptosis and necrosis by 6-OHDA, including caspase activity and lactate dehydrogenase release. Notably, cells exposed to 6-OHDA in the presence of NGF were subsequently capable of proliferation (when replated without NGF), or neurite outgrowth (with continued presence of NGF). Following 7 days growth in the presence of NGF, expression of betaIII tubulin and tyrosine hydroxylase and increased intracellular catecholamines was detectable in PC12 cells, features characteristic of functional dopaminergic neurons. NGF-pre-treated PC12 cells retained expression of betaIII-tubulin and tyrosine hydroxylase, but not catecholamine content following 6-OHDA exposure. These data indicate that NGF-protected cells maintained some aspects of functionality and were subsequently capable of proliferation or differentiation.
authors.affiliation: Department of Biochemistry, National University of Ireland, Galway, Ireland.
authors.forename: Edel T
authors.initials: ET
authors.lastname: Kavanagh
authors.forename: John P
authors.initials: JP
authors.lastname: Loughlin
authors.forename: Kate Reed
authors.initials: KR
authors.lastname: Herbert
authors.forename: Peter
authors.initials: P
authors.lastname: Dockery
authors.forename: Afshin
authors.initials: A
authors.lastname: Samali
authors.forename: Karen M
authors.initials: KM
authors.lastname: Doyle
authors.forename: Adrienne M
authors.initials: AM
authors.lastname: Gorman
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000032:analysis; D002395:Catecholamines / Q000032:analysis; D002454:Cell Differentiation; D049109:Cell Proliferation; D009336:Necrosis; D020932:Nerve Growth Factor / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D016627:Oxidopamine / Q000037:antagonists & inhibitors* / Q000633:toxicity; D016716:PC12 Cells; D051381:Rats; D014404:Tubulin / Q000032:analysis; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D015415:Biomarkers; D002395:Catecholamines; D014404:Tubulin; D016627:Oxidopamine; D020932:Nerve Growth Factor; D014446:Tyrosine 3-Monooxygenase