title: Studies on PVP hydrogel-supported luminol chemiluminescence: 2. Luminometer calibration and potential analytical applications.
abstract: The use of poly(N-vinyl-2-pyrrolidone) (PVP) hydrogel-supported luminol chemiluminescence (CL) for the automatic determination of hydrogen peroxide and the quantification of the antiradical capacity of Trolox is described. The hydrogel containing luminol and hemin is prepared directly on a 96-well microplate and can be stored for up to 3 months without significant decrease in CL quantum yields. Furthermore, this system can also be used as a secondary light standard for the calibration of microplate luminometers.
authors.affiliation: Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, 05508-900 São Paulo, SP, Brazil.
authors.forename: Erick Leite
authors.initials: EL
authors.lastname: Bastos
authors.forename: Luiz Francisco Monteiro Leite
authors.initials: LF
authors.lastname: Ciscato
authors.forename: Fernando Heering
authors.initials: FH
authors.lastname: Bartoloni
authors.forename: Luiz Henrique
authors.initials: LH
authors.lastname: Catalani
authors.forename: Paulete
authors.initials: P
authors.lastname: Romoff
authors.forename: Wilhelm Josef
authors.initials: WJ
authors.lastname: Baader
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001331:Automation; D002138:Calibration; D002839:Chromans / Q000737:chemistry / Q000494:pharmacology; D006427:Hemin / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D008165:Luminol / Q000737:chemistry*; D015394:Molecular Structure; D011205:Povidone / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002839:Chromans; D020100:Hydrogels; D008165:Luminol; D006427:Hemin; D006861:Hydrogen Peroxide; D011205:Povidone; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid