title: New polymorphic microsatellites in Glossina pallidipes (Diptera: Glossinidae) and their cross-amplification in other tsetse fly taxa.
abstract: We report the development and characterization of three new microsatellite markers in the tsetse fly, Glossina pallidipes (Diptera: Glossinidae). Fifty-eight alleles were scored in 192 individuals representing six natural populations. Allelic diversity ranged from 9 to 28 alleles per locus (mean 19.3 +/- 5.5). Averaged across loci, observed heterozygosity was 0.581 +/- 0.209, and expected heterozygosity was 0.619 +/- 0.181. Cross-species amplifications of the G. pallidipes loci in other tsetse fly taxa are reported.
authors.affiliation: Department of Entomology, Iowa State University, Ames, IA 50011-3222, USA.
authors.forename: J O
authors.initials: JO
authors.lastname: Ouma
authors.forename: J G
authors.initials: JG
authors.lastname: Marquez
authors.forename: E S
authors.initials: ES
authors.lastname: Krafsur
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018429:Glossinidae / Q000145:classification / Q000235:genetics*; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D013045:Species Specificity
chemical_list: D017931:DNA Primers