[Effect of carbachol on dendritic cell function in the lipopolysaccharides induced murine sepsis model].
OBJECTIVE
To explore the mechanism of the effect of carbachol in the treatment of sepsis at the angle of modulation of dendritic cell (DC) activity.


METHODS
Thirty male C57BL/6 mice were randomized into three groups: normal control, sepsis and carbachol. The sepsis model of mice was reproduced by the injection of lipopolysaccharides (LPS). The expression of interleukin-1 beta (IL-1 beta) and IL-12 p70 positive cells were measured by immunohistochemistry method. The changes in DC activity in the spleen of mice were studied using flow cytometry.


RESULTS
In LPS group, the number of splenic DC was increased, but it was not statistically significant. The expression of DC molecules including major histocompatibility complex-II (MHC-II), CD86 and the rate of positive cells containing IL-1 beta and IL-12 p70 were significantly higher than those in normal controls (all P<0.05). While in carbachol treatment group, the number of splenic DC had no significant change, the expression of IL-1 beta was down-regulated, dramatically. The expression of MHC-II, CD86 and IL-12 p70 were also lowered (all P<0.05).


CONCLUSIONS
Carbachol can lower the DC activity, suggesting that modulation of DC activity may lower extensive immune and inflammatory response in the early phases of sepsis.
Tian
Guang
G
Department of Pathology, Burn Institute, First Affiliated Hospital of General Hospital of PLA, Beijing 100037, China.
Lu
Jiang-yang
JY
Hu
Sen
S
Lü
Yi
Y
Wang
Hong-wei
HW
Yang
Yi
Y
D000818:Animals; D051940:B7-2 Antigen / Q000276:immunology / Q000378:metabolism; D039521:CD11c Antigen / Q000276:immunology / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology*; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D005802:Genes, MHC Class II / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology / Q000378:metabolism; D053583:Interleukin-1beta / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D018805:Sepsis / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000378:metabolism
D051940:B7-2 Antigen; D039521:CD11c Antigen; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D018664:Interleukin-12; D002217:Carbachol