title: Key components of the fission machinery are interchangeable.
abstract: Brefeldin-A ADP-ribosylated substrate (BARS) and dynamin function in membrane fission in distinct intracellular transport pathways, but whether their functions are mechanistically similar is unclear. Here, we show that ARFGAP1, a GTPase-activating protein (GAP) for ADP-ribosylation factor 1 (ARF1), couples to either BARS or endophilin B for vesicle formation by the coat protein I (COPI) complex - a finding that reveals an unanticipated mechanistic flexibility in mammalian COPI transport. Because dynamin is coupled to endophilin A in vesicle formation by the clathrin-coat complex, our finding also predicts that dynamin and ARF GAPs are likely to be functional counterparts in membrane fission among different transport pathways that connect intracellular membrane compartments.
authors.affiliation: Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Jia-Shu
authors.initials: JS
authors.lastname: Yang
authors.forename: Leiliang
authors.initials: L
authors.lastname: Zhang
authors.forename: Stella Y
authors.initials: SY
authors.lastname: Lee
authors.forename: Helge
authors.initials: H
authors.lastname: Gad
authors.forename: Alberto
authors.initials: A
authors.lastname: Luini
authors.forename: Victor W
authors.initials: VW
authors.lastname: Hsu
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D020755:Coat Protein Complex I / Q000378:metabolism; D018699:Coated Vesicles / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D006056:Golgi Apparatus / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D020755:Coat Protein Complex I; D014157:Transcription Factors; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase