title: Establishment and characterization of a new cell line, FPS-1, derived from human undifferentiated pleomorphic sarcoma, overexpressing epidermal growth factor receptor and cyclooxygenase-2.
abstract: BACKGROUND
  Undifferentiated pleomorphic sarcoma (UPS) is among the most common soft tissue sarcomas in adults. In order to improve its aggressive course or prognosis and establish new therapeutic methods, molecular genetic and biological characterizations of UPS are required.
  
  
  METHODS
  A new human UPS cell line (FPS-1) was established from UPS of the upper arm of a 79-year-old man. The cell line has been maintained for over 14 months with more than 60 passages. FPS-1 cells were characterized using molecular biological methods.
  
  
  RESULTS
  FPS-1 cells showed the same morphological and immunophenotypical characteristics as the primary tumor. Cytogenetic and molecular analyses revealed a nonsense mutation in exon 6 of the p53 gene. Epidermal growth factor receptor (EGFR) and cyclooxygenase-2 (COX-2) were expressed in FPS-1 cells.
  
  
  CONCLUSIONS
  FPS-1 cells might be useful for investigating biological behavior and developing new molecular targeting antitumor drugs for UPS with EGFR or COX-2 expression.
authors.affiliation: First Department of Pathology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima-shi, Fukushima 960-1295, Japan. paco@fmu.ac.jp
authors.forename: Michiyuki
authors.initials: M
authors.lastname: Hakozaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hojo
authors.forename: Michiko
authors.initials: M
authors.lastname: Sato
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tajino
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yamada
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kikuchi
authors.forename: Masafumi
authors.initials: M
authors.lastname: Abe
mesh_terms: D000368:Aged; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007621:Karyotyping; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012509:Sarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012983:Soft Tissue Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000628:therapy
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D066246:ErbB Receptors