title: Structure-based cross-linking of NF-kappaB p50 homodimer and decoy bearing a novel 2'-disulfide trapping site.
abstract: Double-stranded oligodeoxyribonucleotides with engineered disulfide units were successfully used for covalent trapping of cysteine containing proteins. In particular, an efficient cross-linking of NF-kappaB p50 homodimer to a sequence-specific decoy was demonstrated. The results suggest that the synthetic oligonucleotides bearing a novel 2'-disulfide trapping site can be used as new tools to study and manipulate biological systems.
authors.affiliation: Chemistry Department, M.V. Lomonossov Moscow State University, Leninskie Gori, Moscow, Russia.
authors.forename: Valeri
authors.initials: V
authors.lastname: Metelev
authors.forename: Andrey
authors.initials: A
authors.lastname: Romanenkov
authors.forename: Elena
authors.initials: E
authors.lastname: Kubareva
authors.forename: Evgeniy
authors.initials: E
authors.lastname: Zubin
authors.forename: Nikolai
authors.initials: N
authors.lastname: Polouchine
authors.forename: Timofei
authors.initials: T
authors.lastname: Zatsepin
authors.forename: Nikolai
authors.initials: N
authors.lastname: Molochkov
authors.forename: Tatiana
authors.initials: T
authors.lastname: Oretskaya
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry*; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry*; D052002:NF-kappa B p50 Subunit / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D004220:Disulfides; D052002:NF-kappa B p50 Subunit; D009838:Oligodeoxyribonucleotides