Improved resolution in (13)C solid-state spectra through spin-state-selection.
The application of a spin-state-selective coherence transfer experiment (INADEQUATE-SSS) to solid-state NMR spectroscopy is described. Two-dimensional (13)C double-quantum/single-quantum spectra without J splittings in both dimensions lead to enhanced spectral resolution. The method is demonstrated to significantly improve the spectral resolution of the crowded C'-C(alpha) region of two proteins.
Verel
René
R
Physical Chemistry, ETH Zurich, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland.
Manolikas
Theofanis
T
Siemer
Ansgar B
AB
Meier
Beat H
BH
D000465:Algorithms; D002247:Carbon Isotopes / Q000737:chemistry*; D003198:Computer Simulation; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D012680:Sensitivity and Specificity; D013113:Spin Labels
D002247:Carbon Isotopes; D013113:Spin Labels