title: YB-1 is important for an early stage embryonic development: neural tube formation and cell proliferation.
abstract: The eukaryotic Y-box-binding protein-1 (YB-1) is involved in the transcriptional and translational control of many biological processes, including cell proliferation. In clinical studies, the cellular level of YB-1 closely correlates with tumor growth and prognosis. To understand the role of YB-1 in vivo, especially in the developmental process, we generated YB-1 knock-out mice, which are embryonic lethal and exhibit exencephaly associated with abnormal patterns of cell proliferation within the neuroepithelium. beta-Actin expression and F-actin formation were reduced in the YB-1 null embryo and YB-1(-/-) mouse embryonic fibroblasts, suggesting that the neural tube defect is caused by abnormal cell morphology and actin assembly within the neuroepithelium. Fibroblasts derived from YB-1(-/-) embryos demonstrated reduced growth and cell density. A colony formation assay showed that YB-1(-/-) mouse embryonic fibroblasts failed to undergo morphological transformation and remained contact-inhibited in culture. These results demonstrate that YB-1 is involved in early mouse development, including neural tube closure and cell proliferation.
authors.affiliation: Department of Molecular Biology, University of Occupational and Environmental Health, School of Medicine, Yahatanishi-ku, Kitakyushu 807-8555, Japan. uchiumi@med.uoeh-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Uchiumi
authors.forename: Abbas
authors.initials: A
authors.lastname: Fotovati
authors.forename: Takakazu
authors.initials: T
authors.lastname: Sasaguri
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Shibahara
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shimada
authors.forename: Takao
authors.initials: T
authors.lastname: Fukuda
authors.forename: Takanori
authors.initials: T
authors.lastname: Nakamura
authors.forename: Hiroto
authors.initials: H
authors.lastname: Izumi
authors.forename: Teruhisa
authors.initials: T
authors.lastname: Tsuzuki
authors.forename: Michihiko
authors.initials: M
authors.lastname: Kuwano
authors.forename: Kimitoshi
authors.initials: K
authors.lastname: Kohno
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology* / Q000196:embryology*; D020964:Embryo Loss; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology*; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009436:Neural Tube Defects / Q000235:genetics / Q000401:mortality / Q000473:pathology; D051840:Y-Box-Binding Protein 1 / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
chemical_list: D051840:Y-Box-Binding Protein 1