Changes in self-reactive IgG antibody repertoire after treatment of experimental autoimmune encephalomyelitis with anti-allergic drugs.
We reduced EAE severity by using two anti-allergic drugs. A control group of mice received i.p. injections of PBS as vehicle while a further two groups were treated either with pyrilamine, a histamine receptor 1 antagonist or with CV6209, a platelet activating factor receptor antagonist. Our results showed that the blockade of the responses to both histamine and PAF leads together to a decline in clinical signs of EAE and significant changes in the serum IgG recognition of some healthy brain antigenic targets. We characterized two discriminant antigens: internexin neuronal intermediate filament protein, and malate dehydrogenase 1, which were able to clearly distinguish untreated mice from treated mice. Their role as potent targets in pathogenic and/or neuroprotective processes is discussed.
El Behi
Mohamed
M
Laboratoire d'immunologie EA2686, Faculté de Médecine, 1, Place de Verdun, 59045 Lille Cedex, France.
Zéphir
Hélène
H
Lefranc
Didier
D
Dutoit
Virginie
V
Dussart
Patricia
P
Devos
Patrick
P
Dessaint
Jean Paul
JP
Vermersch
Patrick
P
Prin
Lionel
L
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D000888:Antibodies, Anti-Idiotypic / Q000097:blood / Q000276:immunology*; D002490:Central Nervous System / Q000276:immunology; D004586:Electrophoresis; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005260:Female; D006633:Histamine Antagonists / Q000494:pharmacology; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology*; D007381:Intermediate Filament Proteins / Q000276:immunology; D008291:Malate Dehydrogenase / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors; D010980:Platelet Membrane Glycoproteins / Q000037:antagonists & inhibitors; D040901:Proteomics; D011726:Pyridinium Compounds / Q000494:pharmacology; D011738:Pyrilamine / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D018926:Anti-Allergic Agents; D000888:Antibodies, Anti-Idiotypic; D006633:Histamine Antagonists; D006634:Histamine H1 Antagonists; D007074:Immunoglobulin G; D007381:Intermediate Filament Proteins; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D011726:Pyridinium Compounds; D043562:Receptors, G-Protein-Coupled; C046643:alpha-internexin; C039351:platelet activating factor receptor; C053321:CV 6209; D008291:Malate Dehydrogenase; D011738:Pyrilamine