title: Analysis of European and north american maize inbred and hybrid lines by monolithic and perfusion reversed-phase high-performance chromatography and multivariate analysis.
abstract: Monolithic and perfusion RP-HPLC methods have been employed for the separation of maize proteins from several European and North American inbred and hybrid lines in analysis times close to 4 min for the perfusion column and close to 8 min for the monolithic column. A study of the repeatability of the protein extraction conditions and of the perfusion and monolithic RP-HPLC methods was performed, indicating low values of variance for the relative peak areas and for the retention times. On the other hand, a low inter-kernel variability of these chromatographic parameters was also found, confirming the possibility of a variety identification and classification of maize inbred and hybrid lines by using a RP-HPLC analysis of the maize proteins. A multivariate analysis of the chromatographic data was carried out in order to characterize and identify the inbred and hybrid maize lines. Cluster analysis of the data showed how far or closely related were the maize lines. Principal component analysis showed that protein chromatographic data had enough information to distinguish between the different groups of maize lines. Finally, a linear discriminant analysis enabled the correct classification of the inbred and hybrid lines according to their geographical origin showing the best percentage of cases correctly classified for monolithic RP-HPLC.
authors.affiliation: Departamento de Química Analítica e Ingeniería Química, Facultad de Química, Universidad de Alcala, 28871 Alcala de Henares, Madrid, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Rodríguez-Nogales
authors.forename: María Concepción
authors.initials: MC
authors.lastname: Garcia
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Marina
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003433:Crosses, Genetic; D005060:Europe; D015999:Multivariate Analysis; D009656:North America; D010940:Plant Proteins / Q000032:analysis / Q000145:classification / Q000235:genetics*; D003313:Zea mays / Q000737:chemistry / Q000145:classification / Q000235:genetics*
chemical_list: D010940:Plant Proteins