title: Fast LC-MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine.
abstract: A fast method was designed for the simultaneous determination of amphetamine (A), methamphetamine (MA), PMA, MDA, MDMA, MDEA and MBDB in urine. The drugs were analysed by LC (ESI)-MS/MS, after a simple liquid-liquid extraction in the presence of the deuterated analogues. Reverse phase separation on an Atlantis dC18 Intelligent Speed column was achieved in less than 4 min under gradient conditions, and the total run time was 8 min. The method was fully validated, including linearity (1-1000 ng/mL for A, MDMA, MDEA and MBDB; 2-1000 ng/mL for MDA and PMA; 1-200 ng/mL for MA; r2>0.99 for all compounds), recovery (>80%), within-day and between-day precision and accuracy (CV and MRE<12.7% for intermediate level and ULOQ, and <17.2% for LLOQ), limit of detection (0.2 ng/mL for MDMA, MDEA and MBDB; 0.5 ng/mL for A, MA and PMA; 1 ng/mL for MDA) and quantitation (1 ng/mL for A, MA, MDMA, MDEA and MBDB; 2 ng/mL for MDA and PMA) and relative ion intensities. No matrix effect was observed. The procedure proved to be sensitive, specific and rapid, and was applied to real forensic cases.
authors.affiliation: Forensic Toxicology Service, Institute of Legal Medicine, University of Santiago de Compostela, C/San Francisco s/n, 15782 Santiago de Compostela, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Concheiro
authors.forename: Susana Maria dos Santos Sadler
authors.initials: SM
authors.lastname: Simões
authors.forename: Oscar
authors.initials: O
authors.lastname: Quintela
authors.forename: Ana
authors.initials: A
authors.lastname: de Castro
authors.forename: Mário João Rodrigues
authors.initials: MJ
authors.lastname: Dias
authors.forename: Angelines
authors.initials: A
authors.lastname: Cruz
authors.forename: Manuel
authors.initials: M
authors.lastname: López-Rivadulla
mesh_terms: D000293:Adolescent; D000328:Adult; D000662:Amphetamines / Q000652:urine*; D005260:Female; D053593:Forensic Toxicology / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006213:Hallucinogens / Q000652:urine*; D006801:Humans; D008297:Male; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000662:Amphetamines; D006213:Hallucinogens