Determination of active components of Ginkgo biloba in human urine by capillary high-performance liquid chromatography/mass spectrometry with on-line column-switching purification.
Ginkgo biloba is one of the most popular herbal nutritional supplements, with terpene lactones and flavonoids being the two major active components. An on-line purification high-performance liquid chromatography/mass spectrometry (HPLC/MS) method was successfully developed for the quantitative determination of flavonoids and terpene lactones excreted in human urine after ingesting the herbal supplement. Satisfactory separation was obtained using a C18 capillary column made in-house with sample clean-up and pre-concentration achieved using a C18 pre-column with column switching. High selectivity and limits of detection of 1-18 ng/mL were achieved using a selected ion monitoring (SIM) scan in negative ion mode; the on-line solid-phase extraction (SPE) recovery of the active components in Ginkgo biloba determined in this study was greater than 75%.
Ding
Shujing
S
Mass Spectrometry Research Unit, Grove Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.
Dudley
Ed
E
Chen
Lijuan
L
Plummer
Sue
S
Tang
Jiandong
J
Newton
Russell P
RP
Brenton
A Gareth
AG
D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005419:Flavonoids / Q000652:urine*; D020441:Ginkgo biloba / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D010936:Plant Extracts / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016482:Urinalysis / Q000295:instrumentation / Q000379:methods*
D005419:Flavonoids; D010936:Plant Extracts