Ischemic preconditioning and kidney transplantation: in vivo nitric oxide monitoring in a rat ischemia-reperfusion experimental model.
Direct nitric oxide measurement in live tissue would help us to understand its role in ischemia-reperfusion injury and its relationship to ischemic preconditioning (IP). We constructed four experimental groups of ischemia-reperfusion in the rat kidney: G1 were controls; G2, 1 hour of renal ischemia; G3 and G4: one or two 15/10 minute cycles, respectively, of IP prior to 1 hour of ischemia. Real-time in vivo nitric oxide measurements were compared with functional parameters of kidney damage at 24 hours. The peaks of nitric oxide production in the IP periods increased less in the rising curve of nitric oxide production during the 1 hour ischemia time. No improvement in the IP groups was observed based on serum creatinine levels at 24 hours.
Herrero
F
F
Department of Medical and Surgical Science, University of Cantabria, Santander, Spain. herrerop@ono.com
Morales
D
D
Baamonde
C
C
Salas
E
E
Berrazueta
J R
JR
Casanova
D
D
D000818:Animals; D003404:Creatinine / Q000097:blood; D004784:Environmental Monitoring; D019194:Ischemic Preconditioning; D016030:Kidney Transplantation / Q000379:methods* / Q000502:physiology*; D023421:Models, Animal; D009569:Nitric Oxide / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D009569:Nitric Oxide; D003404:Creatinine