[Rh antigen stability of mPEG modified red blood cells].
The objective of study was to investigate the Rh antigen stability of mPEG-modified RBC. RBC membrane protein SDS-PAGE technology was used to analyze the combination of the mPEG modified RBC membrane protein with mPEG molecules; the RBC ghost coagulation test and 4 degrees C CPD-preserved modified RBC mixed with matched blood were used to observe the stability of RBC Rh antigen camouflaged by mPEG. The results showed that the blood groups of stored mPEG-modified RBC were kept consistency before or after simulating transfusion, i.e. mixture of modified RBC with matched bloods, while the plasma hemoglobin after simulating transfusion was not only within the normal range during the storage, but also less than that before simulating transfusion even after incubation at 37 degrees C. The electrophoresis pattern stained with iodine and Coomassie blue displayed the bands of mPEG combined with RBC membrane protein and the slow mobility of membrane protein. The hemagglutination of PEGylation RBC ghosts did not take place and mPEG still covered the antigen. In conclusion, mPEG-SPA can bind the erythrocyte with its extracted membrane protein in both ghosts and living erythrocytes.
Qiu
Yan
Y
Beijing Red Cross Blood Center, Beijing 100088, China. qiu444cn@yahoo.com.cn
Zha
Yi
Y
Tan
Ying-Xia
YX
Zhang
Yang-Pei
YP
D004910:Erythrocyte Membrane / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000097:blood; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D065227:Transfusion Reaction
D007518:Isoantibodies; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol