Biomarker candidate identification in Yersinia pestis using organism-wide semiquantitative proteomics.
The accurate mass and time tag mass spectrometry method and clustering analysis were used to compare the abundance change of 992 Yersinia pestis proteins under four contrasting growth conditions (26 and 37 degrees C, with or without Ca2+) that mimicked growth states in either a flea vector or mammalian host. Eighty-nine proteins were observed to have similar abundance change profiles to 29 known virulence associated proteins, providing identification of additional biomarker candidates. Eighty-seven hypothetical proteins, which clustered into 5 distinct clusters of like-protein abundance change, were identified as unique biomarkers related specifically to growth condition.
Hixson
Kim K
KK
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA.
Adkins
Joshua N
JN
Baker
Scott E
SE
Moore
Ronald J
RJ
Chromy
Brett A
BA
Smith
Richard D
RD
McCutchen-Maloney
Sandra L
SL
Lipton
Mary S
MS
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010449:Peptide Mapping; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D014357:Trypsin; D015010:Yersinia pestis / Q000737:chemistry / Q000254:growth & development*
D001426:Bacterial Proteins; D014357:Trypsin