Satellite cells isolated from adult Hanwoo muscle can proliferate and differentiate into myoblasts and adipose-like cells.
This study examined whether adult bovine muscle satellite cells from 30-month-old Hanwoo cattle are multipotential. The satellite cells were found to have the potential to proliferate and differentiate into myoblasts with the formation of multinucleated cells. In addition, treatment with the peroxisome proliferator activating receptor-gamma (PPARgamma) agonist, rosiglitazone, promoted their trans-differentiation into adipocytes with significant increases in glycerol accumulation and glycerol-3-phosphate dehydrogenase activity. Western blot analysis revealed that increased levels of the adipocyte fatty acid-binding protein, PPARgamma and of CCAAT/enhancer-binding protein were closely related to rosiglitazone-induced differentiation of the cells. These findings demonstrate that satellite cells from adult Hanwoo cattle are multipotent, and that their trans-differentiation into adipocytes can be induced by rosiglitazone.
Kook
Sung-Ho
SH
Institute of Oral Biosciences, Chonbuk National University, Chonju 561-756, Korea.
Choi
Ki-Choon
KC
Son
Young-Ok
YO
Lee
Kyuung-Yeol
KY
Hwang
In-Ho
IH
Lee
Hyun-Jeong
HJ
Chang
Jong-Soo
JS
Choi
In-Ho
IH
Lee
Jeong-Chae
JC
D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D039902:Multipotent Stem Cells; D032446:Myoblasts / Q000166:cytology*; D047495:PPAR gamma / Q000819:agonists; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology*
D047495:PPAR gamma