[Effect of calpain inhibitor I on ikappaBalpha expression and cytokine secretion in RAW264.7 cells attacked with LPS].
OBJECTIVE
To explore the effect of calpain inhibitor I(CI-I) on ikappaBalpha expression and cytokine secretion in RAW264.7 cells after LPS attack.


METHODS
RAW264.7 cells were pretreated with CI-I for 1 h and attacked with LPS. The ikappaBalpha expression and TNF-alpha and IL-6 secretion were detected by Western blot and ELISA, respectively.


RESULTS
CI-I inhibited the decrease of ikappaBalpha expression in RAW264.7 cells treated with LPS. The secretion of both TNF-alpha and IL-6 secretion was notably increased after RAW264.7 cells were treated with LPS for 4 h or 8 h. CI-I and dexamethasone(DEX) inhibited these effects, and the combination of DEX and CI-I had synergistic effect.


CONCLUSIONS
CI-I and DEX can inhibit the decrease of ikappaBalpha expression and prevent TNF-alpha and IL-6 secretion in RAW264.7 cells attacked with LPS, which contributes to the alleviation of cellular injury.
Cheng
Xiao-Gang
XG
Institute of Combined Injury, Laboratory of Combined Injury of State Key Labortory of Trauma, Burn and Combined Injury, Department of Preventive Medcine, Third Military Medical University, Chongqing 400038, China. xiaogangchen@yahoo.com
Su
Yong-Ping
YP
Luo
Cheng-Ji
CJ
Liu
Xiao-Hong
XH
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006023:Glycoproteins / Q000494:pharmacology*; D020672:I-kappa B Proteins / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D016207:Cytokines; D006023:Glycoproteins; D020672:I-kappa B Proteins; D008070:Lipopolysaccharides; C000606205:Nfkbia protein, mouse; D012333:RNA, Messenger; C039304:calpain inhibitors; D000072000:NF-KappaB Inhibitor alpha; D003907:Dexamethasone; D002118:Calcium