title: Evaluation of a serological test (indirect ELISA) for the diagnosis of sarcoptic mange in red foxes (Vulpes vulpes).
abstract: Sarcoptic mange occurs in many parts of the world and is common in populations of domestic and wild canids, including red foxes (Vulpes vulpes). In recent years, an indirect antibody enzyme-linked immunosorbent assay (ELISA), with higher sensitivity and specificity than traditional diagnostic methods, has been successfully applied in the diagnosis of sarcoptic mange in dogs. The same ELISA has also demonstrated specific antibodies to Sarcoptes scabiei in experimentally infected red foxes. The aim of this study was to evaluate the indirect ELISA when used to detect antibodies to S. scabiei in field sera from Swedish red foxes. One cohort of both infected and non-infected red foxes (cohort 1; n = 88), and one cohort of apparently non-infected foxes (cohort 2; n = 67) were examined for skin lesions and presence of S. scabiei by thorough visual examination at autopsy and skin scrapings. Samples of blood-tinted body liquid from the abdomen or thorax cavity were collected and analysed by the indirect ELISA. The relative sensitivity and specificity of the ELISA at different cut-offs (OD values) were estimated by comparing the test results to the infection status as determined by examination and skin scrapings. The highest combination of relative sensitivity and specificity, calculated based on cohort 1, was 95.4 and 100.0%, respectively. These estimates were constant for cut-offs 0.150-0.225, which included the cut-off based on the mean plus three standard deviations of test results from cohort 2 (0.165). It is concluded that this test can be useful in diagnosis and epidemiological studies of S. scabiei infection in red foxes.
authors.affiliation: Department of Parasitology (SWEPAR), National Veterinary Institute, Uppsala, Sweden. set.bornstein@sva.se
authors.forename: Set
authors.initials: S
authors.lastname: Bornstein
authors.forename: Jenny
authors.initials: J
authors.lastname: Frössling
authors.forename: Katarina
authors.initials: K
authors.lastname: Näslund
authors.forename: Göran
authors.initials: G
authors.lastname: Zakrisson
authors.forename: Torsten
authors.initials: T
authors.lastname: Mörner
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D005589:Foxes; D008297:Male; D011237:Predictive Value of Tests; D012520:Sarcoptes scabiei / Q000276:immunology*; D012532:Scabies / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D013548:Sweden
chemical_list: D000906:Antibodies