title: A novel and in situ technique for the quantitative detection of MTBE and benzene degrading bacteria in contaminated matrices.
abstract: A novel and in situ technique is presented here as a better alternative to culture-dependent and PCR-based techniques for the quantitative detection of predominant bacterial species involved in the bioremediation of contaminants. It allowed rapid, specific and in situ identification of Biosep-immobilized eubacteria from MTBE- and benzene-contaminated matrices.
authors.affiliation: University of Tennessee, Knoxville, TN, United States. jbiggers@utk.edu
authors.forename: J P
authors.initials: JP
authors.lastname: Biggerstaff
authors.forename: M
authors.initials: M
authors.lastname: Le Puil
authors.forename: B L
authors.initials: BL
authors.lastname: Weidow
authors.forename: J
authors.initials: J
authors.lastname: Leblanc-Gridley
authors.forename: E
authors.initials: E
authors.lastname: Jennings
authors.forename: J
authors.initials: J
authors.lastname: Busch-Harris
authors.forename: K L
authors.initials: KL
authors.lastname: Sublette
authors.forename: D C
authors.initials: DC
authors.lastname: White
authors.forename: R S
authors.initials: RS
authors.lastname: Alberte
mesh_terms: D001554:Benzene / Q000378:metabolism*; D001673:Biodegradation, Environmental; D020564:Deltaproteobacteria / Q000302:isolation & purification / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008738:Methyl Ethers / Q000032:analysis* / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D008738:Methyl Ethers; D012989:Soil Pollutants; C043243:methyl tert-butyl ether; D001554:Benzene