Measuring the flexibility of immunoglobulin by gold nanoparticles.
We measured the flexibility of Fab and Fc arms of immunoglobulin using gold nanoparticles (GNPs). Enzyme-linked immunosorbent assay was performed to measure the affinity of anti-5 nm GNP antiserum against various sizes of GNPs. The flexibility of Fc was also measured by electron microscopy. The restricted binding affinity indicated that only a very limited amount of freedom was allowed for the Fab-Fab hinge, while Fab-Fc showed a much larger degree of freedom.
Huang
G Steven
GS
Institute of Nanotechnology and Center for Nano Science & Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China. gstevehuang@mail.nctu.edu.tw
Chen
Yu-Shiun
YS
Yeh
Hsiao-Wei
HW
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006046:Gold / Q000737:chemistry*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry*; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D018583:Pliability; D012680:Sensitivity and Specificity
D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D006046:Gold