Immunosuppression of xenograft rejection in porcine kidney PK15 cells by porcine IL-18.
Xenotransplantation, the transplantation of cells, tissues or organs between individuals of different species, would resolve the current shortage of organs, but rejection remains the major hurdle to successful xenotransplantation. In the present study, we analyzed mixed lymphocyte reactions (MLRs) and used 51Cr release assays in order to identify the proliferation and expansion of mouse CD8+ cytotoxic T lymphocyte cells against PK15, PK15/pIL-18 or PK15/mIL-18 cells. In addition, we identified T cell populations in mouse splenocytes and lymph node cells using two-color flow cytometry. It was found that the CD8+ T cells of xenograft recipients proliferated extensively and that the survival rates of populations of PK15/mIL-18 or PK15/pIL-18 cells were higher than untransfected controls. Moreover, CD3+ T cells were increased in mice injected with PK15 cells or PK15/pIL-18 cells but PK15/pIL-18 cell numbers were lower in lymph nodes than untransfected controls. CD8+ T cells numbers were reduced in the lymph nodes of PK15/pIL-18 injected mice. These results suggest that porcine IL-18 regulates anti-pig cellular rejection in C57BL/6 mice.
Choi
Yun Sil
YS
Clinical Biochemistry Laboratory, Department of Pharmacy, College of Pharmacy, Daejeon 305-764, Korea.
Kim
Young Kwan
YK
Shim
Jung Hyun
JH
Kim
Eun Mi
EM
Kang
Hyung Sik
HS
Yoon
Do Young
DY
Muneta
Yoshihiro
Y
Myung
Pyung Keun
PK
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000637:transplantation*; D005260:Female; D005822:Genetic Vectors / Q000138:chemical synthesis; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D007165:Immunosuppression Therapy / Q000379:methods*; D020382:Interleukin-18 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D007668:Kidney / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine; D013601:T-Lymphocytes / Q000378:metabolism; D014018:Tissue Distribution; D014162:Transfection; D019076:Transgenes / Q000276:immunology / Q000502:physiology; D014180:Transplantation; D014183:Transplantation, Heterologous
D016207:Cytokines; D020382:Interleukin-18; D012333:RNA, Messenger