Molecular analysis of poplar defense against herbivory: comparison of wound- and insect elicitor-induced gene expression.
In order to characterize defense responses of hybrid poplar (Populus trichocarpax P. deltoides), we profiled leaf transcript patterns elicited by wounding and by regurgitant from forest tent caterpillar (FTC; Malacosoma disstria), a Lepidopteran defoliator of poplars. Macroarrays were used to compare transcript profiles. Both FTC-regurgitant (FTC-R) and mechanical wounding with pliers elicited expression of a variety of genes, and for these genes our analysis indicated that these treatments induced qualitatively similar responses. Similarly, a comparison of responses of directly treated and systemically induced leaves indicated extensive overlap in the sets of induced genes. FTC-R was found to contain the insect-derived elicitor volicitin. The simulated herbivory treatments resulted in the induction of genes involved in poplar defense and secondary metabolism. We also identified wound-responsive genes with roles in primary metabolism, including a putative invertase, lipase, and acyl-activating enzyme; some of these genes may have roles in defense signaling. In addition, we found three unknown genes containing a ZIM motif which may represent novel transcription factors.
Major
Ian T
IT
Centre for Forest Biology and Department of Biology, University of Victoria, Stn CSC, PO Box 3020, Victoria, BC, V8W 3 N5, Canada.
Constabel
C Peter
CP
D000818:Animals; D020224:Expressed Sequence Tags; D005247:Feeding Behavior / Q000502:physiology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D017343:Genes, Plant; D007814:Larva / Q000502:physiology; D009036:Moths / Q000502:physiology*; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D032107:Populus / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000378:metabolism
D010940:Plant Proteins; D014361:Trypsin Inhibitors