title: Marinobacter vinifirmus sp. nov., a moderately halophilic bacterium isolated from a wine-barrel-decalcification wastewater.
abstract: A halophilic, Gram-negative, motile, non-sporulating bacterium designated strain FB1T was isolated from a wine-barrel-decalcification wastewater. The organism comprises straight rods and has a strictly respiratory metabolism with O2. Strain FB1T grows optimally at 20-30 degrees C and 5-6% NaCl. The predominant fatty acids were found to be C18:1omega9c (30.4%), C16:0 (25.7%), C12:0 3-OH (10.3%), C16:1omega9c (9.7%) and C16:1omega7c (8.4%). A phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain forms a coherent cluster within the genus Marinobacter. The highest level of 16S rRNA gene sequence similarity (97.9%) exhibited by strain FB1T was with the type strain of Marinobacter excellens. However, the level of DNA-DNA relatedness between the novel strain and M. excellens CIP 107,686T was only 31.2%. The DNA G+C content of strain FB1T was 58.7 mol%. On the basis of phenotypic and genotypic characteristics, and also phylogenetic evidence, strain FB1T is considered to represent a novel species of the genus Marinobacter, for which the name Marinobacter vinifirmus sp. nov. is proposed. The type strain is FB1T (=DSM 17747T=CCUG 52119T).
authors.affiliation: IRD, UMR180 MicroBiotech (IRD/Université de Provence/Université de la Méditerranée), Biotechnologie Microbienne des Environnements Chauds, IFR86-IBAIM (INRA/IRD/Université de Provence/Université de la Méditerranée), ESIL, F-13288 Marseille, France.
authors.forename: Pierre-Pol
authors.initials: PP
authors.lastname: Liebgott
authors.affiliation: IRD, UMR180 MicroBiotech (IRD/Université de Provence/Université de la Méditerranée), Biotechnologie Microbienne des Environnements Chauds, IFR86-IBAIM (INRA/IRD/Université de Provence/Université de la Méditerranée), ESIL, F-13288 Marseille, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Casalot
authors.affiliation: IRD, UMR180 MicroBiotech (IRD/Université de Provence/Université de la Méditerranée), Biotechnologie Microbienne des Environnements Chauds, IFR86-IBAIM (INRA/IRD/Université de Provence/Université de la Méditerranée), ESIL, F-13288 Marseille, France.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Paillard
authors.affiliation: IRD, UMR180 MicroBiotech (IRD/Université de Provence/Université de la Méditerranée), Biotechnologie Microbienne des Environnements Chauds, IFR86-IBAIM (INRA/IRD/Université de Provence/Université de la Méditerranée), ESIL, F-13288 Marseille, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Lorquin
authors.affiliation: IRD, UMR180 MicroBiotech (IRD/Université de Provence/Université de la Méditerranée), Biotechnologie Microbienne des Environnements Chauds, IFR86-IBAIM (INRA/IRD/Université de Provence/Université de la Méditerranée), ESIL, F-13288 Marseille, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Labat
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D005516:Food Microbiology; D020459:Genes, rRNA; D005840:Gentian Violet; D007220:Industrial Waste; D053520:Marinobacter / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010100:Oxygen / Q000378:metabolism; D010619:Phenazines; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D007220:Industrial Waste; D010619:Phenazines; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D005840:Gentian Violet; D010100:Oxygen