Ectopic expression of Plk1 leads to activation of the spindle checkpoint.
Polo-like kinase 1 (Plk1), the best characterized member of the mammalian polo-like kinase family, is well regulated throughout the cell cycle, and is inhibited following DNA damage. Chk1 plays a key role in the response to DNA damage. We recently reported that Chk1 is required for mitotic progression through negative regulation of Plk1. Here, we report the phenotypes of cultured cells upon ectopic expression of various forms of Plk1. Epitopic expression of Plk1 led to mitotic arrest, whereas coexpression of Chk1 could release this mitotic block. Moreover, the Plk1 expression-induced mitotic block was also released by inactivation of the spindle-assembly checkpoint.
Tang
Jiabin
J
Department of Biochemistry and the Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA.
Erikson
Raymond L
RL
Liu
Xiaoqi
X
D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000502:physiology*; D049109:Cell Proliferation; D002470:Cell Survival; D000071877:Checkpoint Kinase 1; D002875:Chromosomes / Q000648:ultrastructure; D004249:DNA Damage; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008938:Mitosis; D010641:Phenotype; D011494:Protein Kinases / Q000096:biosynthesis* / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000502:physiology*; D034622:RNA Interference; D008941:Spindle Apparatus; D000097869:Polo-Like Kinase 1
D018797:Cell Cycle Proteins; D000071877:Checkpoint Kinase 1; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1; C000605867:CHEK1 protein, human