Functional genomics of histone modification and non-histone chromosomal proteins using the polytene chromosomes of Drosophila.
Knowing the genomic distribution of chromosomal proteins and of histone modifications provides essential insight into function. The giant polytene chromosomes of the Drosophila larval salivary glands provide a high-resolution genomic map with a resolution exceeded only by chromatin immunoprecipitation. Immunofluorescence localization of chromosomal proteins and specific post-translational modifications of histones is a simple and rapid tool for the functional genomics of chromosomal proteins.
Eissenberg
Joel C
JC
Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, MO 63104, USA. eissenjc@slu.edu
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics; D023281:Genomics / Q000379:methods*; D006657:Histones / Q000378:metabolism*; D012469:Salivary Glands / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; D006657:Histones