title: Characterization of the Caenorhabditis elegans G protein-coupled serotonin receptors.
abstract: Serotonin (5-HT) regulates a wide range of behaviors in Caenorhabditis elegans, including egg laying, male mating, locomotion and pharyngeal pumping. So far, four serotonin receptors have been described in the nematode C. elegans, three of which are G protein-coupled receptors (GPCR), (SER-1, SER-4 and SER-7), and one is an ion channel (MOD-1). By searching the C. elegans genome for additional 5-HT GPCR genes, we identified five further genes which encode putative 5-HT receptors, based on sequence similarities to 5-HT receptors from other species. Using loss-of-function mutants and RNAi, we performed a systematic study of the role of the eight GPCR genes in serotonin-modulated behaviors of C. elegans (F59C12.2, Y22D7AR.13, K02F2.6, C09B7.1, M03F4.3, F16D3.7, T02E9.3, C24A8.1). We also examined their expression patterns. Finally, we tested whether the most likely candidate receptors were able to modulate adenylate cyclase activity in transfected cells in a 5-HT-dependent manner. This paper is the first comprehensive study of G protein-coupled serotonin receptors of C. elegans. It provides a direct comparison of the expression patterns and functional roles for 5-HT receptors in C. elegans.
authors.affiliation: CGMC, CNRS-UMR 5534, Université Claude Bernard Lyon-1, Université Lyon-1, 43 bld du 11 Novembre 1918, 69622 Villeurbanne cedex, France.
authors.forename: Maïté
authors.initials: M
authors.lastname: Carre-Pierrat
authors.forename: David
authors.initials: D
authors.lastname: Baillie
authors.forename: Robert
authors.initials: R
authors.lastname: Johnsen
authors.forename: Rhonda
authors.initials: R
authors.lastname: Hyde
authors.forename: Anne
authors.initials: A
authors.lastname: Hart
authors.forename: Laure
authors.initials: L
authors.lastname: Granger
authors.forename: Laurent
authors.initials: L
authors.lastname: Ségalat
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D019556:COS Cells; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D002522:Chlorocebus aethiops; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D011985:Receptors, Serotonin / Q000235:genetics* / Q000378:metabolism; D043762:Reproductive Behavior; D017386:Sequence Homology, Amino Acid
chemical_list: D029742:Caenorhabditis elegans Proteins; D043562:Receptors, G-Protein-Coupled; D011985:Receptors, Serotonin