title: Immunohistochemical analysis of ZnT1, 4, 5, 6, and 7 in the mouse gastrointestinal tract.
abstract: Expression of five zinc transporters (ZnT1, 4, 5, 6, and 7) of the Slc30 family in the mouse gastrointestinal tract was studied by immunohistochemical analysis. Results demonstrated unique expression patterns, levels, and cellular localization among ZnT proteins in the mouse gastrointestinal tract with some overlapping. ZnT1 was abundantly expressed in the epithelium of the esophagus, duodenum of the small intestine, and cecum of the large intestine. ZnT4 was predominantly detected in the large intestine. ZnT5 was mainly expressed in the parietal cell of the stomach and in the absorptive epithelium of the duodenum and jejunum. ZnT6 was predominantly detected in the chief cell of the stomach, columnar epithelial cells of the jejunum, cecum, colon, and rectum. Lastly, ZnT7 was observed in all epithelia of the mouse gastrointestinal tract with the highest expression in the small intestine. Expression of ZnT proteins in the absorptive epithelial cell of the gastrointestinal tract suggests that ZnT proteins may play important roles in zinc absorption and endogenous zinc secretion.
authors.affiliation: Department of Nutrition, University of California at Davis, Davis, California, USA.
authors.forename: Yan Yiu
authors.initials: YY
authors.lastname: Yu
authors.forename: Catherine P
authors.initials: CP
authors.lastname: Kirschke
authors.forename: Liping
authors.initials: L
authors.lastname: Huang
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D004848:Epithelium / Q000378:metabolism; D004947:Esophagus / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000033:anatomy & histology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000378:metabolism; D007420:Intestine, Large / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D026901:Membrane Transport Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D015032:Zinc / Q000378:metabolism*
chemical_list: D027682:Cation Transport Proteins; D026901:Membrane Transport Proteins; C485803:Slc30a1 protein, mouse; C109053:Slc30a4 protein, mouse; C486753:Slc30a5 protein, mouse; C472293:ZnT7 protein, mouse; D015032:Zinc