Long-term haematopoietic reconstitution and survival without interleukin-7 in a murine syngeneic bone marrow transplantation model.
We created a syngeneic mouse bone marrow transplantation (BMT) model to examine the effect of endogenous interleukin-7 (IL-7) on long-term (>or=140 days) haematopoietic reconstitution and survival after BMT. Wild-type (WT) IL-7(+/+) and knockout (KO) IL-7(-/-) mice were lethally irradiated and transplanted with bone marrow. Survival is best (85.7%) in the group WT grafts transplanted into WT recipients (WT-->WT) with a trend towards poorer survival in the other groups (WT-->KO: 60%, KO-->WT: 50%, KO-->KO: 45.5%, differences statistically not significant). If the recipient is deficient for IL-7-producing cells, T- and B-cell reconstitution remain incomplete. If the graft lacks IL-7-producing cells there is a significant delay in T- and NK-cell reconstitution. Interestingly, in the absence of IL-7, T-cell reconstitution is neither delayed nor incomplete because of an expansion of TCRalphabeta(+)/CD4(-)/CD8(-) double negative T cells. Long-term survival and lymphocyte reconstitution after syngeneic BMT can occur despite the absence of IL-7.
Feyen
O
O
Department of Pediatric Oncology, Hematology and Immunology, University Children's Hospital Düsseldorf, Düsseldorf, Germany.
Göbel
U
U
Schneider
D T
DT
Burdach
S E G
SE
Niehues
T
T
D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology; D016026:Bone Marrow Transplantation; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D006413:Hematopoietic System / Q000166:cytology / Q000502:physiology; D015851:Interleukin-7 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D007694:Killer Cells, Natural / Q000737:chemistry / Q000166:cytology; D038041:Lymphopoiesis / Q000235:genetics*; D051379:Mice / Q000235:genetics / Q000502:physiology*; D018345:Mice, Knockout; D023421:Models, Animal; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D013601:T-Lymphocytes / Q000737:chemistry / Q000166:cytology
D015704:CD4 Antigens; D016827:CD8 Antigens; D015851:Interleukin-7; D016693:Receptors, Antigen, T-Cell, alpha-beta