title: [DNA amplification of Plasmodium vivax parasites from Giemsa-stained blood smears].
abstract: OBJECTIVE
  To develop methods of extracting DNA from malaria parasites on Giemsa-stained blood smears.
  
  
  METHODS
  Improved Na2HPO4 method and Chelex-100 ion-exchange technique were used to extract DNA from Giemsa-stained or unstained blood smears. Nested PCR was employed for amplification and identification of allelotype in the Plasmodium vivax merozoite surface protein-1 (PvMSP-1).
  
  
  RESULTS
  Target DNA bands appeared in all samples of unstained thick blood smears, while no DNA bands were visible in the fixed and stained thin smears. Both methods identified PvMSP-1 alleles from smears with parasitemia of > or = 0.01%.
  
  
  CONCLUSIONS
  It is feasible to identify PvMSP-1 alleles from Giemsa-stained blood smear.
authors.affiliation: Fujian Provincial Center for Diseases Control and Prevention, Fuzhou, China.
authors.forename: Fang-zhen
authors.initials: FZ
authors.lastname: Xiao
authors.forename: Shan-ying
authors.initials: SY
authors.lastname: Zhang
authors.forename: Long-shan
authors.initials: LS
authors.lastname: Xu
authors.forename: Jiang-hong
authors.initials: JH
authors.lastname: Huang
authors.forename: Han-guo
authors.initials: HG
authors.lastname: Xie
authors.forename: Yang-rong
authors.initials: YR
authors.lastname: Ou
mesh_terms: D000483:Alleles; D000818:Animals; D001399:Azure Stains; D016054:DNA, Protozoan / Q000097:blood*; D006801:Humans; D010966:Plasmodium vivax / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D001399:Azure Stains; D016054:DNA, Protozoan