title: Direct visualization of neo-vessel formation following peripheral injection of bone marrow derived CD34+ cells in experimental myocardial damage.
abstract: The definitive fate of peripherally injected PKH26 labelled bone marrow mononuclear cells expressing the CD34+ antigen following experimental myocardial cryodamage in rats (n=10) has been examined by direct visualization on photoconverted light and electron microscopy images. One week after the injection in each rat of about 150,000 CD34+ cells early stage PKH26+ vascular structures were localized in the infarcted areas, suggesting that a potential benefit of this therapeutic approach consists in the regeneration of the vasculature.
authors.affiliation: Istituto di Medicina Cardiovascolare, Centro di Fisiologia Clinica e Ipertensione, University of Milan, IRCCS Ospedale Maggiore Policlinico, Via F. Sforza, 35-20122 Milano, Italy. michele.ciulla@unimi.it
authors.forename: M M
authors.initials: MM
authors.lastname: Ciulla
authors.forename: S
authors.initials: S
authors.lastname: Ferrero
authors.forename: U
authors.initials: U
authors.lastname: Gianelli
authors.forename: R
authors.initials: R
authors.lastname: Paliotti
authors.forename: F
authors.initials: F
authors.lastname: Magrini
authors.forename: P
authors.initials: P
authors.lastname: Braidotti
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis*; D001854:Bone Marrow Cells / Q000737:chemistry / Q000502:physiology*; D016026:Bone Marrow Transplantation; D007150:Immunohistochemistry; D008853:Microscopy; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy*; D018919:Neovascularization, Physiologic; D051381:Rats; D013997:Time Factors
chemical_list: D018952:Antigens, CD34