Expression of an Nkx3.1-CRE gene using ROSA26 reporter mice.
The genetic locus of Nkx3.1, an early murine marker of sclerotome and prostate development, was disrupted by a knock in of CRE recombinase via homologous recombination in embryonic stem cells. Cell fate mapping revealed previously unidentified cell lineages expanded from Nkx3.1-expressing cell populations and recapitulated reported Nkx3.1 expression patterns. In lineage trace experiments of E18.5 Nkx3.1-CRE; R26R embryos novel staining was observed in areas of the lungs, portions of the duodenum, and vertebral elements of the skeleton. beta-galactosidase activity measured in Nkx3.1-CRE; R26R and Nkx3.2-CRE; R26R embryos was observed in overlapping regions of the sclerotome but no apparent change in Nkx3.1 expression was seen in the Nkx3.2 mutants by in situ hybridization.
Stanfel
M N
MN
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA.
Moses
K A
KA
Carson
J A
JA
Zimmer
D B
DB
DeMayo
F
F
Schwartz
R J
RJ
Zimmer
W E
WE
D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage / Q000235:genetics*; D017931:DNA Primers; D004386:Duodenum / Q000378:metabolism; D053595:Embryonic Stem Cells; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism*; D017403:In Situ Hybridization; D008168:Lung / Q000378:metabolism; D051379:Mice; D013131:Spine / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D001616:beta-Galactosidase
D017931:DNA Primers; D018398:Homeodomain Proteins; C102837:Nkx3-1 protein, mouse; D014157:Transcription Factors; D001616:beta-Galactosidase