Differential expression of phospholipases D1 and D2 in mouse tissues.
The differential expression of phospholipase D (PLD) isozymes, which include PLD1 and PLD2, was examined in various murine tissues, including the cerebrum, cerebellum, heart, lung, liver, spleen, stomach, pancreas, ileum, colon, adrenal gland, kidneys, testes, ovaries, and uterus. In Western blot analysis, only PLD1 was detected in the heart and ovary, while only PLD2 was detected in the pancreas and ileum. Both PLD1 and PLD2 were strongly expressed in the cerebrum, cerebellum, and lung, and both were also expressed in the liver, spleen, stomach, colon, kidney, testes, and uterus. Immunohistochemistry showed intense PLD immunostaining in the cerebrum, cerebellum, lungs, intestines, and testis, and weak PLD immunostaining in the liver, kidneys, spleen, and heart. These findings suggest that PLD1 and PLD2 are differentially expressed in the various organs of mice, and that each PLD isozyme plays a distinct role in each organ.
Kim
Heechul
H
Department of Veterinary Medicine, Cheju National University, Jejudaehakro 66, Aradong, Jeju city, Jeju-do 690-756, South Korea.
Lee
Jeeyoung
J
Kim
Seungjoon
S
Shin
Min Kyoung
MK
Min
Do Sik
do S
Shin
Taekyun
T
D000818:Animals; D015153:Blotting, Western; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology; D005260:Female; D041981:Gastrointestinal Tract / Q000166:cytology / Q000201:enzymology; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D032383:Myocytes, Cardiac / Q000166:cytology / Q000201:enzymology; D009928:Organ Specificity; D010739:Phospholipase D / Q000235:genetics* / Q000378:metabolism*; D021381:Protein Transport; D013687:Telencephalon / Q000166:cytology / Q000201:enzymology; D014566:Urogenital System / Q000166:cytology / Q000201:enzymology
C105606:phospholipase D2; D010739:Phospholipase D; C106801:phospholipase D1