title: Early injection of OP-1 during distraction osteogenesis accelerates new bone formation in rabbits.
abstract: Distraction osteogenesis (DO) is a surgical technique for generating new bone by applying controlled distraction of two bony segments post osteotomy. A limitation of the technique is the long time required for the new bone to consolidate. We investigated the effect of injecting osteogenic protein 1 (OP-1) at the beginning of distraction in a rabbit model of DO. Regenerate bone was evaluated using radiology, densitometry, micro-computed tomography (microCT) and histomorphometry. Immunohistochemsitry was used to evaluate changes in expression of various ligands, growth factors and receptors following OP-1 treatment. Compared to the control, a two-fold increase in bone volume was apparent for treated groups at 3 weeks post injection. An upregulation of almost all of the 41 genes examined was observed. Results suggested that applying OP-1 early during distraction can accelerate bone formation by the activation of numerous pathways. This study provides further insights on strategies to improve bone regeneration rate in DO.
authors.affiliation: Shriners Hospital, Montreal Children Hospital, Division of Orthopaedics, McGill University, Montreal, Que., Canada.
authors.forename: Manuela
authors.initials: M
authors.lastname: Mandu-Hrit
authors.forename: Tasima
authors.initials: T
authors.lastname: Haque
authors.forename: Dominique
authors.initials: D
authors.lastname: Lauzier
authors.forename: Maria
authors.initials: M
authors.lastname: Kotsiopriftis
authors.forename: Frank
authors.initials: F
authors.lastname: Rauch
authors.forename: Maryam
authors.initials: M
authors.lastname: Tabrizian
authors.forename: Janet E
authors.initials: JE
authors.lastname: Henderson
authors.forename: Reggie C
authors.initials: RC
authors.lastname: Hamdy
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D019857:Osteogenesis, Distraction / Q000379:methods*; D011817:Rabbits; D011859:Radiography; D013977:Tibia / Q000000981:diagnostic imaging / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D036341:Intercellular Signaling Peptides and Proteins; D016212:Transforming Growth Factor beta