title: Immune enhancing effects of recombinant bovine IL-18 on foot-and-mouth disease vaccination in mice model.
abstract: Foot-and-mouth disease (FMD) is a highly contagious disease in cloven-hoofed animals and can cause a considerable socio-economic loss for affected countries. Interleukin-18 (IL-18) is a pleiotropic cytokine and plays important role in both the development of a functional immune system as well as the response of the organism to infection. In the present study, bovine IL-18 (BoIL-18), Foot-and-mouth disease virus VP1 and VP1/BoIL-18 fusion genes were cloned and expressed in pichia pastoris (P. pastoris) and subsequently immune effects were evaluated to study the immune enhancing effects of recombinant BoIL-18 (rBoIL-18) on FMD vaccination. The results showed that the genes encoding for BoIL-18, VP1 and VP1/BoIL-18 are successfully expressed in P. pastoris and the expressed recombinant VP1 (rVP1) proteins could induce both humoral and marginal cell-mediated immune responses in mice, while the co-inoculation with rBoIL-18 could markedly enhance both of immune responses, and the inoculation of the fusion product rVP1/BoIL-18 showed even more dramatic immune responses, suggesting rBoIL-18 has a potential to enhance the efficacy of vaccination against FMDV infection.
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing 100094, PR China.
authors.forename: Xi-Ju
authors.initials: XJ
authors.lastname: Shi
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D049109:Cell Proliferation; D003001:Cloning, Molecular; D016207:Cytokines / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis; D007202:Indicators and Reagents; D020382:Interleukin-18 / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism; D014614:Vaccines, Synthetic / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D020382:Interleukin-18; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines