[Study of fetal lymphocyte of intrahepatic cholestasis of pregnancy].
OBJECTIVE
To explore effect of fetal lymphocyte on pathogenesis of intrahepatic cholestasis of pregnancy (ICP).


METHODS
Twenty pregnant women with ICP and 20 normal pregnant women were enrolled in the study. The single mixed lymphocyte culture/reaction (MLC/MLR) was conducted using inactive lymphocyte obtained from maternal peripheral blood and lymphocyte of cord blood from fetus. Antigen-induced-lymphocyte-proliferation-reaction was used for dermic soluble antigen and decidual soluble antigen obtained from maternal blood and cord blood from fetus. The intense of proliferation was calculated and compared between normal and ICP-complicated pregnancies.


RESULTS
(1) The level of intense of proliferation of fetal lymphocyte was significantly increased in ICP group 2.75 +/- 0.36 than those of normal control group 1.45 +/- 0.19 in single mixed lymphocyte culture (P < 0.05). (2) The level of intense of proliferation of fetal lymphocyte was significantly increased in ICP group 1.45 +/- 0.19 than those of normal control group 0.67 +/- 0.24 in decidual soluble antigen induced lymphocyte proliferation reaction (P < 0.05). (3) The level of intense of proliferation of fetal lymphocyte was significantly increased in ICP group (1.22 +/- 0.44) than those of normal control group (0.66 +/- 0.27) in dermic soluble antigen induced lymphocyte proliferation reaction.


CONCLUSIONS
(1) The fetal lymphocyte may be one of the effector cells in pathogenesis of ICP. (2) The disturbance of fatal-maternal immune-tolerance is one of the important mechanisms underlying ICP.
Qin
Lang
L
Department of Obstetrics and Gynecology, Second Affiliated Hospital and Immunological of Sichuan University, Chengdu 610041, China.
Liu
Shu-Yun
SY
Xing
Ai-Yun
AY
Tan
Zong-Jian
ZJ
Cai
Mei-Ying
MY
Zhao
Zhong-Rong
ZR
D000328:Adult; D049109:Cell Proliferation; D002478:Cells, Cultured; D002780:Cholestasis, Intrahepatic / Q000097:blood / Q000276:immunology*; D003656:Decidua / Q000276:immunology; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood / Q000276:immunology*