title: [Detection of serum hepatitis B virus large envelope protein and its relationship with viral replication.].
abstract: BACKGROUND
  To explore the significance of HBV large envelope protein (LHBs) in diagnosing HBV replication in chronic hepatitis B patients.
  
  
  METHODS
  Serum HBV DNA was quantitively detected by using real-time polymerase chain reaction (RT-PCR), the LHBs and Pre-S1 were detected by using enzyme linked immunosorbent assay (ELISA) and HBV markers were detected by time differentiate immunofluorescence assay in 340 serum samples collected from chronic hepatitis B patients.
  
  
  RESULTS
  Serum LHBs level was closely correlated with number of HBV DNA copies (r=0.899, P=0.0380). There was no significant difference between positive rate of LHBs and that of HBV DNA in different HBeAg pattern (P>0.05); the positive rate of LHBs was 83.15%, which was higher than that of Pre-S1 and HBeAg which were 50.54% and 54.48%, respectively. There was significant difference (P less than 0.05).
  
  
  CONCLUSIONS
  The level of serum LHBs can be used to estimate the state of HBV replication and the sensitivity was superior to both Pre-S1 and HBeAg. So it may be used as a new serological marker to detect HBV replication.
authors.affiliation: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou 313000, China. Corresponding author: DAI Li-cheng, E-mail: dlc@hzhospital.com.
authors.forename: L C
authors.initials: LC
authors.lastname: Dai
authors.forename: C N
authors.initials: CN
authors.lastname: Wang
authors.forename: J F
authors.initials: JF
authors.lastname: He
authors.forename: J P
authors.initials: JP
authors.lastname: Qian
authors.forename: W H
authors.initials: WH
authors.lastname: Wang
authors.forename: B N
authors.initials: BN
authors.lastname: Shi
mesh_terms: D004279:DNA, Viral / Q000097:blood; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics; D019694:Hepatitis B, Chronic / Q000821:virology; D006801:Humans; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D006513:Hepatitis B e Antigens