Mouse hepatitis virus does not induce Beta interferon synthesis and does not inhibit its induction by double-stranded RNA.
Mouse hepatitis virus (MHV) does not induce interferon (IFN) production in fibroblasts or bone marrow-derived dendritic cells. In this report, we show that the essential IFN-beta transcription factors NF-kappaB and IFN regulatory factor 3 are not activated for nuclear translocation and gene induction during infection. However, MHV was unable to inhibit the activation of these factors and subsequent IFN-beta production induced by poly(I:C). Further, MHV infection did not inhibit IFN-beta production mediated by known host pattern recognition receptors (PRRs) (RIG-I, Mda-5, and TLR3). These results are consistent with the notion that double-stranded RNA, produced during MHV infection, is not accessible to cellular PRRs.
Zhou
Haixia
H
Department of Microbiology, University of Iowa, Bowen Science Building 3-730, Iowa City, IA 52242, USA.
Perlman
Stanley
S
D000818:Animals; D002460:Cell Line; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D050838:Interferon Regulatory Factor-3 / Q000235:genetics / Q000378:metabolism; D000072640:Interferon-Induced Helicase, IFIH1; D016899:Interferon-beta / Q000096:biosynthesis*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D006517:Murine hepatitis virus / Q000145:classification / Q000276:immunology / Q000472:pathogenicity*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D011070:Poly I-C / Q000494:pharmacology; D012330:RNA, Double-Stranded / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011956:Receptors, Cell Surface; D051196:Toll-Like Receptor 3 / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D000097905:Roundabout Proteins
D053487:DEAD-box RNA Helicases; D050838:Interferon Regulatory Factor-3; D000072640:Interferon-Induced Helicase, IFIH1; D016899:Interferon-beta; D008565:Membrane Proteins; D016328:NF-kappa B; D009419:Nerve Tissue Proteins; D011070:Poly I-C; D012330:RNA, Double-Stranded; D011956:Receptors, Cell Surface; D051196:Toll-Like Receptor 3; C118348:Robo3 protein, mouse; D000097905:Roundabout Proteins; C510592:Ifih1 protein, mouse