title: Potato tuber proteomics: comparison of two complementary extraction methods designed for 2-DE of acidic proteins.
abstract: Two protein extraction procedures were tested in order to remove interfering compounds prior to 2-DE of potato tubers. These methods using SDS lysis buffer and phenol-phase extraction were compared regarding the quality of the resulting 2-D gel. While the resolution of SDS extracts on semipreparative gels seems better, both methods lead to similar extraction yields and total number of spots. The procedures are complementary regarding the Mr range of preferentially extracted proteins.
authors.affiliation: Plant Biology Unit, Gembloux Agricultural University, Gembloux, Belgium. delaplace.p@fsagx.ac.be
authors.forename: Pierre
authors.initials: P
authors.lastname: Delaplace
authors.forename: Froukje
authors.initials: F
authors.lastname: van der Wal
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Dierick
authors.forename: Jan H G
authors.initials: JH
authors.lastname: Cordewener
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fauconnier
authors.forename: Patrick
authors.initials: P
authors.lastname: du Jardin
authors.forename: Antoine H P
authors.initials: AH
authors.lastname: America
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D019800:Phenol; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D035281:Plant Tubers / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012967:Sodium Dodecyl Sulfate; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D010940:Plant Proteins; D019800:Phenol; D012967:Sodium Dodecyl Sulfate