Mice deficient in Dmrt7 show infertility with spermatogenic arrest at pachytene stage.
Genes including DM domain regulate sexual development in diverse metazoan phyla. One of these genes, Dmrt7, was expressed only in testes of adult mice. To determine the role of Dmrt7 in mice, we generated Dmrt7-knockout mice (Dmrt7-/-). Although the Dmrt7-/- showed normal growth, null males were infertile. No sperm was detected in the epididymis of Dmrt7-/- adult males. Absence of spermatids in a histological analysis, decreased expression of Ccna1 mRNA and the accumulation of SCP3-positive spermatocytes showed the arrest of spermatogenesis at the pachytene stage in the Dmrt7-knockout mice.
Kawamata
Masaki
M
Department of Molecular Biology, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
Nishimori
Katsuhiko
K
D000818:Animals; D018797:Cell Cycle Proteins; D019925:Cyclin A / Q000096:biosynthesis; D056750:Cyclin A1; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000378:metabolism*; D004822:Epididymis / Q000166:cytology / Q000378:metabolism; D007248:Infertility, Male / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009687:Nuclear Proteins / Q000378:metabolism; D049471:Pachytene Stage / Q000502:physiology*; D013087:Spermatids / Q000166:cytology / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000172:deficiency / Q000378:metabolism*
C503747:Ccna1 protein, mouse; D018797:Cell Cycle Proteins; D019925:Cyclin A; D056750:Cyclin A1; D004268:DNA-Binding Proteins; C486538:Dmrt2 protein, mouse; D009687:Nuclear Proteins; C490062:Sycp3 protein, mouse; D014157:Transcription Factors