title: Some factors affecting successful vitrification of mouse blastocysts.
abstract: The effects of temperature and exposure time to vitrification solutions on In vitro survival of mouse blastocysts were investigated. Blastocysts were first exposed for 10 min to vitrification Solution 1 (VS1) containing 10% glycerol-20% 1,2 propanediol in phosphate buffered saline (PBS), then to vitrification Solution 2 (VS2) with 25 % glycerol-25% 1,2 propanediol for various periods either at room temperature or at 4 degrees C. At room temperature survival dropped quickly, while at 4 degrees C an increase in survival was observed. It is concluded that the viability of mouse blastocyts after vitrification is dependent on the temperature and duration of equilibration in vitrification solutions.
authors.affiliation: Department of Animal Reproduction, Faculty of Veterinary Medicine, Brussels, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Van Der Zwalmen
authors.forename: B
authors.initials: B
authors.lastname: Gaurois
authors.forename: F J
authors.initials: FJ
authors.lastname: Ectors
authors.forename: K
authors.initials: K
authors.lastname: Touati
authors.forename: A
authors.initials: A
authors.lastname: Massip
authors.forename: F
authors.initials: F
authors.lastname: Ectors