title: Development and application of RAPD-SCAR marker for identification of Phyllanthus emblica LINN.
abstract: Correct genotype identification of medicinal plant material remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. The present study was carried out to develop DNA based marker for identification of Phyllanthus emblica LINN. A putative marker (1.1 kb) specific for P. emblica was identified by Random Amplified Polymorphic DNA (RAPD) technique. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon. The SCAR marker was found useful for identification of P. emblica in its commercial samples and Triphalachurna, a multi-component Ayurvedic formulation.
authors.affiliation: Bioprospecting Laboratory, Interdisciplinary School of Health Sciences, University of Pune, India.
authors.forename: Warude
authors.initials: W
authors.lastname: Dnyaneshwar
authors.forename: Chavan
authors.initials: C
authors.lastname: Preeti
authors.forename: Joshi
authors.initials: J
authors.lastname: Kalpana
authors.forename: Patwardhan
authors.initials: P
authors.lastname: Bhushan
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D029803:Phyllanthus / Q000145:classification / Q000235:genetics; D029842:Phyllanthus emblica / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers