Duodenal diazepam-binding protein expression and plasma cholecystokinin after alcoholic pancreatitis.
OBJECTIVE
After the first acute alcohol-induced pancreatitis (AAIP) episode 46 % of patients will have a recurrent attack, but the pathophysiology is unclear. The hyperstimulation of the pancreas with cholecystokinin (CCK) induces acute pancreatitis. Alcohol induces temporary stimulation of the pancreas and CCK could be a mediator. CCK is regulated by releasing peptides - diazepam-binding protein (DBI) being a possible candidate. The aim of this study was to investigate the possible association between CCK plasma levels and DBI expression in patients with AAIP or its recurrence.


METHODS
The study comprised 44 subjects (mean age 42 years): A) Patients with a first episode of AAIP (n = 9); B) patients with three or more episodes of AAIP (n = 11); C) patients with a heavy alcohol consumption, with no detected AAIP (n = 11) and D) healthy controls (n = 13). CCK levels were measured by radioimmunoassay (RIA). Duodenal biopsies were analyzed for DBI mRNA and histology.


RESULTS
There was no significant difference in CCK plasma levels, DBI expression or CCK/DBI ratio between the groups.


CONCLUSIONS
There were no changes in fasting CCK plasma levels or DBI expression. This may suggest that they do not play a major role as risk factors for alcohol-induced pancreatitis.
Pelli
H
H
Department of Gastroenterology and Alimentary Tract Surgery, Tampere University Hospital, Tampere, Finland.
Herzig
K-H
KH
Uotila
S
S
Räty
S
S
Laine
S
S
Sand
J
J
Nordback
I
I
D000328:Adult; D000368:Aged; D000437:Alcoholism / Q000097:blood / Q000378:metabolism*; D002756:Cholagogues and Choleretics / Q000097:blood; D002766:Cholecystokinin / Q000097:blood*; D026261:Diazepam Binding Inhibitor / Q000378:metabolism*; D004386:Duodenum / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019512:Pancreatitis, Alcoholic / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism*; D010865:Pilot Projects; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002756:Cholagogues and Choleretics; D026261:Diazepam Binding Inhibitor; D012333:RNA, Messenger; D002766:Cholecystokinin