Effect of 2-deoxy-D-glucose on various malignant cell lines in vitro.
BACKGROUND
2-Deoxy-D-glucose (2-DG) is an analog of glucose that is preferentially captured by tumors and is accumulated in transformed cells, because the phosphorylated molecule (2-DG-6P) cannot be metabolized or diffused outside the cells. Targeted with a fluorine atom, 18F-DG is currently used to visualize malignant tumors (PET scan). Although cancer cells have been reported to be strongly dependent on glycolysis (Warburg effect), very few reports have studied the inhibitory effects of 2-DG on cancer.


METHODS
Our objective was to study, in a large panel of human malignant cells of various origins (ovarian, squamous, cerebral, hepatic, colonic and mesothelial), if the inhibitory activity of 2DG against tumor growth could be considered a general phenomenon and to determine its effect on the cell cycle.


RESULTS
Four types of response in the different cell lines were observed when cells were cultured in the presence of 2-DG (5 mM) continuous exposure: proliferation slow down; proliferation arrest without signs of apoptosis; strong cell cycle arrest accompanied by moderate apoptosis induction; massive apoptosis.


CONCLUSIONS
2-DG appears as an interesting new therapeutic agent against cancer in vitro, and should be tested in in vivo studies.
Zhang
Xiao Dong
XD
Groupe Régional d'Etudes sur le Cancer (Grecan), Unité "Biologie et Thérapies Innovantes des Cancers Localement Agressifs", CLCC François Baclesse, Caen, France. x.zhang@baclesse.fr
Deslandes
Edwige
E
Villedieu
Marie
M
Poulain
Laurent
L
Duval
Marilyne
M
Gauduchon
Pascal
P
Schwartz
Laurent
L
Icard
Philippe
P
D000963:Antimetabolites / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003847:Deoxyglucose / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000963:Antimetabolites; D003847:Deoxyglucose; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3