title: [Study on influence of processing methods on chemical constituents in Radix Paeoniae Alba].
abstract: OBJECTIVE
  The influence of processing methods on chemical constituents in Radix Paeoniae Alba was observed.
  
  
  METHODS
  A HPLC method was used for analyzing the changes of eight major constituents, namely gallic acid, paeoniflorin sulfonate, catechin, paeoniflorin sulfonate, albiflorin, paeoniflorin, benzoic acid, pentagalloylglucose and benzoylpaeoniflorin, with the three processing procedures of decorticating, boiling and fumigating by burning of sulphur. Analysis was performed using a Zorbax SB-C18 column (4.6 mm x 250 mm, 5 microm) with the mixture of acetonitrile (A) and 0.015% phosphoric acid solution as mobile phase in gradient mode. The detection wavelength was set at 230 nm and the column temperature was at 30 degrees C.
  
  
  RESULTS
  Except for gallic acid and pentagalloylglucose, the other constituents decreased during procedure of decorticating and boiling. Fumigating by burning of sulphur would produce a new compound, paeoniflorin sulfonate, which was a byproduct from the reaction of paeoniflorin with SO2.
  
  
  CONCLUSIONS
  The significant changes were produced in chemical constituents of Radix Paeoniae Alba during three processing procedures. Therefore, the processing of Radix Paeoniae Alba should be strictly controlled and standardized.
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Peking University, Beijing 100083, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
authors.forename: Rong-Xia
authors.initials: RX
authors.lastname: Liu
authors.forename: Hong-Zhu
authors.initials: HZ
authors.lastname: Guo
authors.forename: Zhong-Ning
authors.initials: ZN
authors.lastname: Zhu
authors.forename: Kai-Shun
authors.initials: KS
authors.lastname: Bi
authors.forename: De-An
authors.initials: DA
authors.lastname: Guo
mesh_terms: D001565:Benzoates / Q000032:analysis* / Q000737:chemistry; D001952:Bridged-Ring Compounds / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005707:Gallic Acid / Q000032:analysis*; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry; D006358:Hot Temperature; D047348:Hydrolyzable Tannins / Q000032:analysis*; D015394:Molecular Structure; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013455:Sulfur; D013678:Technology, Pharmaceutical / Q000379:methods; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D005707:Gallic Acid; D005960:Glucosides; D047348:Hydrolyzable Tannins; D039821:Monoterpenes; D013455:Sulfur; C015423:peoniflorin; D006027:Glycosides; C435084:pentagalloylglucose