Methionine synthase reductase polymorphisms are associated with serum osteocalcin levels in postmenopausal women.
Homocysteine (Hcy) is thought to play an important role in the development of osteoporosis and fracture. Methionine synthase reductase (MTRR) is an enzyme involved in the conversion of Hcy to methionine. We hypothesized that certain genetic polymorphisms of MTRR leading to reduced enzyme activity may cause hyperhomocysteinemia and affect bone metabolism. We therefore examined the associations of the A66G and C524T polymorphisms of the MTRR gene with bone mineral density (BMD) and serum osteocalcin levels in postmenopausal women. Although we did not detect any significant associations between MTRR polymorphisms and BMD or serum osteocalcin levels, we found that the 66G/524C haplotype, which has reduced enzyme activity, was significantly associated with serum osteocalcin levels in a gene-dose dependent manner (P = 0.002). That is, the highest osteocalcin levels (34.5 +/- 16.8 ng/ml) were observed in subjects bearing two copies, intermediate osteocalcin levels (32.6 +/- 14.4 ng/ml) were observed in subjects bearing one copy, and the lowest levels of osteocalcin (28.8 +/- 10.9 ng/ml) were observed in subjects bearing no copies. These results suggest that the 66G/524C haplotype of the MTRR gene affect bone turn over rate.
Kim
Duk Jae
DJ
Division of Endocrinology and Metabolism, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Korea.
Park
Byung Lae
BL
Koh
Jung-Min
JM
Kim
Ghi Su
GS
Kim
Lyoung Hyo
LH
Cheong
Hyun Sup
HS
Shin
Hyoung Doo
HD
Hong
Jung-Min
JM
Kim
Tae-Ho
TH
Shin
Hong-In
HI
Park
Eui Kyun
EK
Kim
Shin-Yoon
SY
D000368:Aged; D000369:Aged, 80 and over; D015519:Bone Density; D005260:Female; D005272:Femur Neck / Q000000981:diagnostic imaging; D005287:Ferredoxin-NADP Reductase / Q000235:genetics* / Q000502:physiology; D005838:Genotype; D006801:Humans; D008161:Lumbosacral Region / Q000000981:diagnostic imaging; D008875:Middle Aged; D015675:Osteocalcin / Q000097:blood*; D011110:Polymorphism, Genetic; D017698:Postmenopause / Q000097:blood*; D011859:Radiography
D015675:Osteocalcin; C111241:methionine synthase reductase; D005287:Ferredoxin-NADP Reductase