[In vitro detection of immune sera to Plasmodium falciparum chimeric protein-2.9 for inhibition of parasite growth by LDH method].
OBJECTIVE
To investigate the in vitro inhibition effect of the rabbit serum immunized with P. falciparum chimeric protein on the parasites by lactate dehydrogenase (LDH) method.


METHODS
Sera of rabbits immunized with P. falciparum chimeric protein were used as test sample, with an initial parasitemia of (0.4 +/- 0.1)% and 2% hematocrit suspension. After 40-42 h of cultivation, LDH assay and a traditional microscopical method were applied to obtain the parasite inhibition rate and infection rate of erythrocytes.


RESULTS
Sera of rabbits immunized with the protein showed a strong in vitro inhibition effect on P. falciparum. There was no significant difference of inhibition rates detected by the two methods (97% and 100% respectively) (P>0.05).


CONCLUSIONS
The LDH method applied in the in vitro inhibition assay for P. falciparum seems simple, reliable and has generated a satisfactory result.
Qu
Li
L
Department of Etiologic Biology, The Second Military Medical University, Shanghai 200433, China.
Pan
Wei-qing
WQ
D000818:Animals; D002135:Calcium-Binding Proteins / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000276:immunology / Q000302:isolation & purification*; D011494:Protein Kinases / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D011817:Rabbits
D002135:Calcium-Binding Proteins; D007106:Immune Sera; D015800:Protozoan Proteins; D007770:L-Lactate Dehydrogenase; D011494:Protein Kinases; C079866:PfCPK protein, Plasmodium falciparum