title: Isolation and expression profiling of genes upregulated in bone marrow-derived mononuclear cells of rheumatoid arthritis patients.
abstract: We have comprehensively identified the genes whose expressions are augmented in bone marrow-derived mononuclear cells (BMMC) from patients with Rheumatoid Arthritis (RA) as compared with BMMCs from Osteoarthritis (OA) patients, and named them AURA after augmented in RA. Both stepwise subtractive hybridization and microarray analyses were used to identify AURA genes, which were confirmed by northern blot analysis and/or reverse transcription polymerase chain reaction (RT-PCR). We also assessed their expression levels in individual patients by quantitative real-time RT-PCR. Of 103 AURA genes we have identified, the mRNA levels of the following 10 genes, which are somehow related to immune responses, were increased in many of the RA patients: AREG (=AURA9), FK506-binding protein 5 (FKBP5 = AURA45), C-type lectin superfamily member 9 (CLECSF9 = AURA24), tyrosylprotein sulfotransferase 1 (TPST1 = AURA52), lymphocyte G0/G1 switch gene (G0S2 = AURA8), chemokine receptor 4 (CXCR4 = AURA86), nuclear factor-kappa B (NF-kappaB = AURA25) and two genes of unknown function (FLJ11106 = AURA1, BC022398 = AURA2 and XM_058513 = AURA17). Since AREG was most significantly increased in many of the RA patients, we subjected it to further analysis and found that AREG-epidermal growth factor receptor signaling is highly activated in synovial cells isolated from RA patients, but not in OA synoviocytes. We propose that the expression profiling of these AURA genes may improve our understanding of the pathogenesis of RA.
authors.affiliation: Center of Arthroplasty, Kyowakai Hospital, Suita, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Nakamura
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Shimaoka
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tougan
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Onda
authors.forename: Daisuke
authors.initials: D
authors.lastname: Okuzaki
authors.forename: Hanjun
authors.initials: H
authors.lastname: Zhao
authors.forename: Azumi
authors.initials: A
authors.lastname: Fujimori
authors.forename: Norikazu
authors.initials: N
authors.lastname: Yabuta
authors.forename: Ippei
authors.initials: I
authors.lastname: Nagamori
authors.forename: Akie
authors.initials: A
authors.lastname: Tanigawa
authors.forename: Jun
authors.initials: J
authors.lastname: Sato
authors.forename: Takenori
authors.initials: T
authors.lastname: Oda
authors.forename: Kenji
authors.initials: K
authors.lastname: Hayashida
authors.forename: Ryuji
authors.initials: R
authors.lastname: Suzuki
authors.forename: Masao
authors.initials: M
authors.lastname: Yukioka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nojima
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ochi
mesh_terms: D066258:Amphiregulin; D001172:Arthritis, Rheumatoid / Q000235:genetics*; D001854:Bone Marrow Cells / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000302:isolation & purification*; D066255:EGF Family of Proteins; D020869:Gene Expression Profiling / Q000379:methods*; D005796:Genes / Q000235:genetics; D006023:Glycoproteins / Q000502:physiology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology; D007962:Leukocytes / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D010003:Osteoarthritis / Q000235:genetics; D013582:Synovial Fluid / Q000166:cytology; D015854:Up-Regulation
chemical_list: C587848:AREG protein, human; D066258:Amphiregulin; D018076:DNA, Complementary; D066255:EGF Family of Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins