title: Effects of red wine on ochratoxin A toxicity in intestinal Caco-2/TC7 cells.
abstract: Ochratoxin A (OTA) is found in a variety of foods and beverages, including red wine. OTA was reported to be nephrotoxic, immunotoxic, hepatotoxic and a potential carcinogen, with yet uncharacterized mechanisms. Consumption of contaminated wines might contribute up to 13% of OTA daily human intake. Potentially chronic exposure has therefore raised public health concern. OTA toxicity in the presence of de-alcoholated red wine was investigated in human intestinal Caco-2/TC7 cells, differentiated on filter supports, by measuring tight junction (TJ) permeability, morphological alterations of TJ proteins and occurrence of apoptosis. Cells were treated with OTA, in the presence of de-alcoholated red wine, for 48h and the ability to recover from the effects of OTA was evaluated after 24h in complete medium. OTA treatment increased TJ permeability and caused intracellular redistribution of claudin-4. However, cells were able to restore permeability and correct localization of claudin-4 following 24h recovery. Conversely, in the presence of red wine, OTA produced faster and irreversible increase in TJ permeability, intracellular delocalization of claudin-4 and extensive apoptosis. Our results point at a possible synergy between OTA and some red wine components, such as polyphenols, in the induction of apoptotic cell death.
authors.affiliation: National Research Institute on Food and Nutrition, Rome, Italy. ranaldi@inran.it
authors.forename: G
authors.initials: G
authors.lastname: Ranaldi
authors.forename: E
authors.initials: E
authors.lastname: Mancini
authors.forename: S
authors.initials: S
authors.lastname: Ferruzza
authors.forename: Y
authors.initials: Y
authors.lastname: Sambuy
authors.forename: G
authors.initials: G
authors.lastname: Perozzi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D018938:Caco-2 Cells; D062506:Claudin-4; D017097:Electric Impedance; D005506:Food Contamination; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis; D009793:Ochratoxins / Q000633:toxicity*; D010539:Permeability; D010750:Phosphoproteins / Q000032:analysis; D019108:Tight Junctions / Q000187:drug effects; D014920:Wine / Q000009:adverse effects*; D062826:Zonula Occludens-1 Protein
chemical_list: C568157:CLDN4 protein, human; D062506:Claudin-4; D008565:Membrane Proteins; D009793:Ochratoxins; D010750:Phosphoproteins; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; C025589:ochratoxin A