Glycerol nucleoside triphosphates: synthesis and polymerase substrate activities.
[Structure: see text] The synthesis of (S)-glycerol nucleoside triphosphates (gNTPs) and the analysis of their substrate activities for enzymatic polymerization is described. NTPs with simplified carbohydrate backbones such as the tNTPs (alpha-L-threose-NTPs) are polymerase substrates and offer the potential to create non-natural aptamer sequences with simplified backbones through enzymatic means. The acyclic (S)-GNA was modeled after the shortened alpha-threofuranosyl backbone. Here we describe the synthesis of (S)-glycerol NTPs and initial enzymatic testing of this further simplified nucleic acid backbone.
Horhota
Allen T
AT
Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, and Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
Szostak
Jack W
JW
McLaughlin
Larry W
LW
D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005990:Glycerol / Q000737:chemistry*; D015394:Molecular Structure; D009705:Nucleosides / Q000737:chemistry*; D013379:Substrate Specificity
D009705:Nucleosides; D004259:DNA-Directed DNA Polymerase; D005990:Glycerol