Direct visualization of neo-vessel formation following peripheral injection of bone marrow derived CD34+ cells in experimental myocardial damage.
The definitive fate of peripherally injected PKH26 labelled bone marrow mononuclear cells expressing the CD34+ antigen following experimental myocardial cryodamage in rats (n=10) has been examined by direct visualization on photoconverted light and electron microscopy images. One week after the injection in each rat of about 150,000 CD34+ cells early stage PKH26+ vascular structures were localized in the infarcted areas, suggesting that a potential benefit of this therapeutic approach consists in the regeneration of the vasculature.
Ciulla
M M
MM
Istituto di Medicina Cardiovascolare, Centro di Fisiologia Clinica e Ipertensione, University of Milan, IRCCS Ospedale Maggiore Policlinico, Via F. Sforza, 35-20122 Milano, Italy. michele.ciulla@unimi.it
Ferrero
S
S
Gianelli
U
U
Paliotti
R
R
Magrini
F
F
Braidotti
P
P
D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis*; D001854:Bone Marrow Cells / Q000737:chemistry / Q000502:physiology*; D016026:Bone Marrow Transplantation; D007150:Immunohistochemistry; D008853:Microscopy; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy*; D018919:Neovascularization, Physiologic; D051381:Rats; D013997:Time Factors
D018952:Antigens, CD34