title: Bacillus foraminis sp. nov., isolated from a non-saline alkaline groundwater.
abstract: A low-G+C-content Gram-positive bacterium, designated CV53T, phylogenetically related to species of the genus Bacillus, was isolated from a highly alkaline non-saline groundwater environment (pH 11.4). This organism comprised rod-shaped cells, was aerobic, did not display spore formation, was catalase- and oxidase-negative, had an optimum growth temperature of 40 degrees C and had an optimum pH of approximately 7.0-8.5. Optimal growth was observed in the absence of NaCl, but growth did occur at NaCl concentrations up to 3.0%. The strain possessed an A1gamma-type peptidoglycan cell wall and the major respiratory quinone was MK-7. The predominant fatty acids were anteiso-C15:0, iso-C15:0 and anteiso-C17:0. The G+C content of the DNA was 43.1 mol%. Phylogenetic analyses of the 16S rRNA gene sequence revealed that the novel isolate is closely related to the type strain of Bacillus jeotgali, forming a coherent cluster supported by bootstrap analysis at a confidence level of 90%. The pairwise similarity of the 16S rRNA gene sequences of the two strains is 97.7%. On the basis of the phylogenetic analyses and the distinct phenotypic characteristics, strain CV53T represents a novel species within the genus Bacillus, for which we propose the name Bacillus foraminis sp. nov. The type strain is CV53T (=LMG 23174T=CIP 108889T).
authors.affiliation: Centro de Neurociências de Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.|Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Igor
authors.initials: I
authors.lastname: Tiago
authors.affiliation: Centro de Neurociências de Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.|Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Carlos
authors.initials: C
authors.lastname: Pires
authors.affiliation: Centro de Neurociências de Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.|Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Vítor
authors.initials: V
authors.lastname: Mendes
authors.affiliation: Instituto do Ambiente e Vida, Universidade de Coimbra, 3004-517 Coimbra, Portugal.|Departamento de Bioquímica, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Paula V
authors.initials: PV
authors.lastname: Morais
authors.affiliation: Departamento de Bioquímica, Universidade de Coimbra, 3004-517 Coimbra, Portugal.|Centro de Neurociências de Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Milton S
authors.initials: MS
authors.lastname: da Costa
authors.affiliation: Centro de Neurociências de Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.|Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: António
authors.initials: A
authors.lastname: Veríssimo
mesh_terms: D000332:Aerobiosis; D001407:Bacillus / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D020459:Genes, rRNA; D006131:Growth Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000032:analysis / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D011809:Quinones / Q000032:analysis / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006131:Growth Inhibitors; D010457:Peptidoglycan; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D010088:Oxidoreductases; D002374:Catalase