A new technique for producing recombinant baculovirus directly in silkworm larvae.
A new technique for the direct production of recombinant baculovirus in the silkworm larvae is described. To assess the utility of this method, a combination of Bombyx mori nucleopolyhedroviral genome, transfer vector and Lipofectin was co-injected directly into newly ecdysed fifth instar, silkworm larvae. The recombinant virus was obtained from the hemolymph of injected larvae and the hemolymph then re-injected into the larvae as an inoculum. This resulted in a high-level production of foreign protein in the silkworm larvae. This technique produces easy and rapid recombinant protein production in silkworms.
Lee
Kwang Sik
KS
College of Natural Resources and Life Science, Dong-A University, Busan, Korea. brjin@dau.ac.kr
Kim
Bo Yeon
BY
Je
Yeon Ho
YH
Woo
Soo Dong
SD
Sohn
Hung Dae
HD
Jin
Byung Rae
BR
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D012831:Bombyx / Q000235:genetics* / Q000821:virology*; D024861:Genetic Enhancement / Q000379:methods; D007814:Larva / Q000235:genetics / Q000821:virology; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014162:Transfection / Q000379:methods*; D014776:Virus Cultivation / Q000379:methods*
D011994:Recombinant Proteins