title: Identification of the FHL2 transcriptional coactivator as a new functional target of the E7 oncoprotein of human papillomavirus type 16.
abstract: We identified the transcriptional coactivator FHL2 as a novel target of the human papillomavirus type 16 (HPV-16) E7 oncoprotein, which plays a major role in cell transformation. The interaction with FHL2 is abolished by mutations in conserved regions 1 and 2 and in the C-terminal zinc finger domain of E7, all required for its transforming potential. We found that E7 impairs the coactivator function of FHL2 on both beta-catenin/LCF-dependent and AP-1-dependent promoters. Thus, the interaction with HPV-16 E7 leads to a promoter-specific impairment of FHL2 function and this may contribute to cell transformation.
authors.affiliation: Tyrolean Cancer Research Institute at the Medical University Innsbruck, 6020 Innsbruck, Austria.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Campo-Fernández
authors.forename: Dieter
authors.initials: D
authors.lastname: Morandell
authors.forename: Frédéric R
authors.initials: FR
authors.lastname: Santer
authors.forename: Werner
authors.initials: W
authors.lastname: Zwerschke
authors.forename: Pidder
authors.initials: P
authors.lastname: Jansen-Dürr
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002472:Cell Transformation, Viral; D018398:Homeodomain Proteins / Q000378:metabolism*; D052162:Human papillomavirus 16 / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D060850:LIM-Homeodomain Proteins; D051379:Mice; D009124:Muscle Proteins / Q000378:metabolism*; D009154:Mutation; D041681:NIH 3T3 Cells; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C105987:FHL2 protein, human; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; D009124:Muscle Proteins; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D014157:Transcription Factors; C059731:oncogene protein E7, Human papillomavirus type 16