Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides.
Various plant polysaccharide derived mono- and oligosaccharides were derivatized with the fluorescent 9-aminopyrene-1,4,6-trisulfonate (APTS) and subjected to capillary electrophoresis (CE) in combination with laser induced fluorescence (LIF) detection. CE-LIF was suitable for mol-based quantification of various APTS-monosaccharides. CE-LIF of APTS-oligosaccharides showed high resolutions, while analysis times were at maximum 15 min. The coupling of CE to electrospray-iontrap mass spectrometery (MS) with online UV detection showed to be a powerful technique in the identification of APTS-oligosaccharides. For the first time, various APTS-xylo-oligosaccharides, having either no, O-acetyl, arabinosyl or xylosyl substitutions at varying positions, were identified by using CE-LIF and CE-MS(n).
Kabel
Mirjam A
MA
Wageningen University, Department of Agrotechnology and Food Sciences, Laboratory of Food Chemistry, Bomenweg 2, 6703 HD Wageningen, The Netherlands.
Heijnis
Walter H
WH
Bakx
Edwin J
EJ
Kuijpers
René
R
Voragen
Alphons G J
AG
Schols
Henk A
HA
D001089:Arabinose / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D009844:Oligosaccharides / Q000032:analysis / Q000302:isolation & purification; D010944:Plants / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis*; D011721:Pyrenes / Q000032:analysis* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013451:Sulfonic Acids / Q000032:analysis* / Q000302:isolation & purification; D014466:Ultraviolet Rays; D014994:Xylose / Q000031:analogs & derivatives
C492698:9-aminopyrene-1,4,6-trisulfonic acid; D009844:Oligosaccharides; D011134:Polysaccharides; D011721:Pyrenes; D013451:Sulfonic Acids; D014994:Xylose; D001089:Arabinose