Methods and platforms for the quantification of splice variants' expression.
The relatively limited number of human protein encoding genes highlights the importance of the diversity generated at the level of the mRNA transcripts. As alternative RNA splicing plays a key role in mediating this diversity, it becomes critical to develop the tools and platforms that will deliver quantitative information on the specific expression levels associated with splice isoforms. This chapter describes the constraints generated by this global transcriptome analysis and the state-of-the-art techniques and products available to the scientific community.
Bracco
Laurent
L
ExonHit Therapeutics, 65 Boulevard Masséna, F-75013 Paris.
Throo
Emeline
E
Cochet
Olivier
O
Einstein
Richard
R
Maurier
Florence
F
D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D005091:Exons / Q000235:genetics; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D046228:Microarray Analysis; D015335:Molecular Probes; D013194:Staining and Labeling
D015335:Molecular Probes