Plant proteomics and glycosylation.
In plant cells, as in other eucaryotic cells, glycosylation is one of the most studied posttranslational events. It can be of two types, N- or O-glycosylation, depending on the linkage involved between the protein backbone and the oligosaccharide moiety. In this review, we present different methods, commonly used in our laboratory, to study the glycosylation of plant proteins. These approaches rely on blot detection with glycan-specific probes, as well as specific deglycosylation of the glycoproteins, followed by mass spectrometry analysis. Such experiments not only allow determination of whether the protein is a glycoprotein, but also how and where it is glycosylated. The last part of this chapter is dedicated to the specific purification and identification of glycoprotein populations in plant cells, so-called glycoproteomics.
Fitchette
Anne-Catherine
AC
CNRS UMR 6037, IFRMP 23, GDR 2590, Université de Rouen, Mont Saint-Aignan, France.
Dinh
Olivia Tran
OT
Faye
Loïc
L
Bardor
Muriel
M
D015153:Blotting, Western; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D006031:Glycosylation; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
D006023:Glycoproteins; D010940:Plant Proteins