CellProfiler: image analysis software for identifying and quantifying cell phenotypes.
Biologists can now prepare and image thousands of samples per day using automation, enabling chemical screens and functional genomics (for example, using RNA interference). Here we describe the first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler. CellProfiler can address a variety of biological questions quantitatively, including standard assays (for example, cell count, size, per-cell protein levels) and complex morphological assays (for example, cell/organelle shape or subcellular patterns of DNA or protein staining).
Carpenter
Anne E
AE
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Jones
Thouis R
TR
Lamprecht
Michael R
MR
Clarke
Colin
C
Kang
In Han
IH
Friman
Ola
O
Guertin
David A
DA
Chang
Joo Han
JH
Lindquist
Robert A
RA
Moffat
Jason
J
Golland
Polina
P
Sabatini
David M
DM
D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D007091:Image Processing, Computer-Assisted; D008957:Models, Genetic; D009154:Mutation; D010641:Phenotype; D015203:Reproducibility of Results; D012984:Software