[Introduction of transpozon Tn9 to endogenic plasmids of Erwinia carotovora during lysogenization of cells by coliphage P1].
It has been shown that phage P1 of Escherichia coli is able not only to lysogenize the cells of Erwinia carotovora but also to be a source of transpozon Tn9 for mutagenesis of cryptic plasmids of this important phytopathogen. Since the amount of the introduction in one of plasmids pCA25::Tn9 is 3.8 kb, at an average, it is supposed that Tn9 is inherited as a double tandem structure. A convenient method is offered for selecting bacterial clones carrying the plasmid pCA25::Tn9 which is based on counterselection of P1-lysogens at high concentrations of chloramphenicol - 100-120 microg/ml.
Sergeeva
Zh Iu
ZhIu
Burova
L M
LM
Tovkach
F I
FI
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017101:Bacteriophage P1 / Q000235:genetics*; D002701:Chloramphenicol / Q000494:pharmacology; D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008242:Lysogeny / Q000235:genetics*; D016973:Pectobacterium carotovorum / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D002701:Chloramphenicol