Molecular cloning and expression analysis of rice OsTVLP1, encoding a protein with similarity to TGF-beta receptor interacting proteins and vacuolar assembly Vam6p/Vps39p proteins.
We describe the cloning and identification of a rice cDNA, OsTVLP1, encoding a protein with similarity to TGF-beta receptor interacting proteins and vacuolar assembly Vam6p/Vps39p proteins. OsTVLP1 has an open reading frame of 2955 bp, which encodes a 984 amino acid protein, containing a citron homology (CNH) domain at its N-terminal and a clathrin heavy-chain repeat homology (CLH) domain at its C-terminal. The expression of OsTVLP1 was induced by treatments with benzothiadiazole (BTH), a chemical activator of plant disease resistance responses, and by infection of the blast fungus, Magnaporthe grisea. Importantly, the expression of OsTVLP1 was activated specifically in disease resistance response induced by BTH and in an incompatible interaction between rice and the blast fungus. Our observations suggest that OsTVLP1 may play a role in rice disease resistance response against pathogen infection.
Hu
Xuebo
X
Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, People's Republic of China.
Song
Fengming
F
Zheng
Zhong
Z
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics; D017434:Protein Structure, Tertiary; D018125:Receptors, Transforming Growth Factor beta; D016415:Sequence Alignment; D013830:Thiadiazoles / Q000494:pharmacology; D014617:Vacuoles / Q000378:metabolism
D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D010940:Plant Proteins; D018125:Receptors, Transforming Growth Factor beta; D013830:Thiadiazoles; C015700:benzo-1,2,3-thiadiazole