Preclinical imaging of mammary intraepithelial neoplasia with positron emission tomography.
Small-animal imaging with positron emission tomography (PET) has become a valuable tool for evaluating preclinical models of breast cancer and other diseases. In this review, we examine a number of issues related to preclinical imaging studies with PET, using transgenic models of ductal carcinoma in situ and metastasis as specific examples. We discuss imaging components such as reconstruction, normalization, and extraction of quantitative parameters. We also analyze the effect of longitudinal correlations on cohort size and present some simple statistical techniques for determining cohort sizes that may be helpful in designing preclinical imaging studies. We describe studies that are greatly facilitated by access to non-invasive imaging data including a study involving multiple endpoints and another investigating metastasis. We conclude with a brief survey of emerging approaches in small-animal PET imaging.
Abbey
Craig K
CK
Department of Biomedical Engineering, University of California, Davis, CA 95616, USA. Abbey@psych.ucsb.edu
Borowsky
Alexander D
AD
Gregg
Jeffery P
JP
Cardiff
Robert D
RD
Cherry
Simon R
SR
D000818:Animals; D002278:Carcinoma in Situ / Q000000981:diagnostic imaging*; D018270:Carcinoma, Ductal, Breast / Q000000981:diagnostic imaging* / Q000556:secondary*; D004195:Disease Models, Animal; D018450:Disease Progression; D019788:Fluorodeoxyglucose F18; D008137:Longitudinal Studies; D008207:Lymphatic Metastasis; D008321:Mammary Glands, Animal / Q000000981:diagnostic imaging / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000473:pathology; D051379:Mice; D049268:Positron-Emission Tomography / Q000379:methods* / Q000639:trends*; D014915:Whole-Body Counting / Q000295:instrumentation / Q000639:trends
D019788:Fluorodeoxyglucose F18