title: Lack of toxic effect of technical azadirachtin during postnatal development of rats.
abstract: Azadirachtin, a biopesticide has been evaluated for its possible toxic effects during postnatal development of rats over two generations. Rats were fed 100, 500 and 1000ppm technical azadirachtin through diet which is equivalent to 5, 25 and 50mg/kg body weight of rats. Technical azadirachtin has not produced any adverse effects on reproductive function and data were comparable to control animals over two generations. There were no toxicological effect in parent rats as evidenced by clinical signs of toxicity, enzymatic parameters like AST, ALT, ALP, S. bilirubin, S. cholesterol, total protein and histopathology of liver, brain, kidney and testes/ovary. The litters of F(1B) and F(2B) generations were devoid of any morphological, visceral and teratological changes. The percent cumulative loss and growth index of pups were also comparable to respective controls in successive growth period of 0, 4, 7, 14 and 21 days in two generations. There were no major malformations in fetuses while some insignificant minor skeletal variations like missing 5th sternebrae and bipartite thoracic centre found were not compound or dose related. No significant pathomorphological changes were observed in liver, kidney, brain and gonads of F(2B) pups. In conclusion rats fed technical azadirachtin showed no evidence of cumulative effects on postnatal development and reproductive performance over two generations. Absence of any major adverse reproductive effects in adults as well as in 21 days old pups of F(2B) generation suggest the safe use of technical azadirachtin as a biopesticide.
authors.affiliation: Pesticide Toxicology Laboratory, Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Lucknow, India.
authors.forename: M K
authors.initials: MK
authors.lastname: Srivastava
authors.forename: R B
authors.initials: RB
authors.lastname: Raizada
mesh_terms: D000818:Animals; D031601:Azadirachta; D001921:Brain / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005333:Fetus / Q000187:drug effects*; D005989:Glycerides / Q000008:administration & dosage / Q000633:toxicity; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects; D036701:Limonins / Q000008:administration & dosage / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D018811:Maternal Exposure; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects*; D013729:Terpenes / Q000008:administration & dosage / Q000633:toxicity; D013737:Testis / Q000187:drug effects
chemical_list: D005989:Glycerides; D007306:Insecticides; D036701:Limonins; D013729:Terpenes; C002443:neem oil; C010329:azadirachtin