title: Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration.
abstract: Cancer cell motility and invasion are critical targets for anticancer therapeutics. Whereas in vitro models could be designed for rapid screening with a view to investigate these targets, careful consideration must be given to the construction of appropriate model systems. Most investigations focus on two-dimensional (2-D) assays despite the fact that increasing evidence suggests that migration across rigid and planar substrates fails to recapitulate in vivo behavior. In contrast, few systems enable three-dimensional (3-D) cell migration to be quantitatively analyzed. We previously developed a digital holographic microscope (DHM) working in transmission with a partially spatial coherence source. This configuration avoids the noise artifacts of laser illumination and makes possible the direct recording of information on the 3-D structure of samples consisting of multiple objects embedded in scattering media, such as cell cultures in matrix gels. The software driving our DHM system is equipped with a time-lapse ability that enables the 3-D trajectories of living cells to be reconstituted and quantitatively analyzed.
authors.affiliation: Department of Physical Chemistry, Microgravity Research Center, Faculty of Applied Sciences, Université Libre de Bruxelles, Brussels, Belgium 1050.
authors.forename: Frank
authors.initials: F
authors.lastname: Dubois
authors.forename: Catherine
authors.initials: C
authors.lastname: Yourassowsky
authors.forename: Olivier
authors.initials: O
authors.lastname: Monnom
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Legros
authors.forename: Olivier
authors.initials: O
authors.lastname: Debeir
authors.forename: Philippe
authors.initials: P
authors.lastname: Van Ham
authors.forename: Robert
authors.initials: R
authors.lastname: Kiss
authors.forename: Christine
authors.initials: C
authors.lastname: Decaestecker
mesh_terms: D002452:Cell Count / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D045744:Cell Line, Tumor; D002465:Cell Movement; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005354:Fibrosarcoma / Q000473:pathology*; D006696:Holography / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D009361:Neoplasm Invasiveness; D010363:Pattern Recognition, Automated / Q000379:methods*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*