title: Effects of addition of sodium lauryl sulfate on frozen-thawed canine spermatozoa.
abstract: The addition of Orvus ES paste (OEP) to extender may be essential for preparing frozen dog semen. The major ingredient of OEP is sodium lauryl sulfate (SLS). In this study, we compared the effect of SLS on frozen dog semen with that of OEP. There were no significant differences between the 2-mg/ml SLS group and OEP group concerning sperm motility, viability and the percentage of viable sperm with intact acrosomes after freeze-thawing. These results suggest that the effectiveness of frozen dog semen extender containing 2 mg/ml of SLS is similar effective to that demonstrated for OEP.
authors.affiliation: Department of Reproduction, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hori
authors.forename: Hanae
authors.initials: H
authors.lastname: Kaseki
authors.forename: Youko
authors.initials: Y
authors.lastname: Fukuhara
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Oba
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Mizutani
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kawakami
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Tsutsui
mesh_terms: D000177:Acrosome / Q000502:physiology; D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000502:physiology*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012967:Sodium Dodecyl Sulfate; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D012967:Sodium Dodecyl Sulfate