title: Sensitivity and specificity of immunoglobulin G titer for the diagnosis of mumps virus in infected patients depending on vaccination status.
abstract: The aim of the study was to evaluate the usefulness of serological detection of mumps IgM and titration of IgG in patients with acute parotitis according to their vaccination status. The detection of mumps virus RNA in saliva by RT-PCR was used as reference. 116 patients (109 of them previously vaccinated) with mumps RT-PCR-negative results and 21 (19 vaccinated) with mumps RT-PCR-positive results were studied. Mumps-specific IgM and IgG were assayed by EIA (Enzygnost, Dade Behring, Germany). IgM results were expressed as positive or negative. For IgG, several cut-offs were calculated using receiver operating characteristic (ROC) curves. Seven RT-PCR-positive and five RT-PCR-negative patients showed IgM-positive results (sensitivity 33.3% and specificity 95.7%). Among vaccinated patients, the sensitivity and specificity of IgM were 26.3% (5/19) and 99.1% (108/109). For IgG, a titer of 5,000 in all the patients showed a sensitivity of 76.2% (16/21) and a specificity of 83.6% (97/116). In vaccinated patients, the corresponding figures for this cut-off were 84.2% (16/19) and 83.5% (91/109), respectively. Although IgM detection against mumps is highly specific, its sensitivity is very low in immunized subjects. In this group, the titration of IgG could serve as an additional diagnostic tool.
authors.affiliation: Laboratorio Regional de Salud Pública, Instituto de Salud Pública de la Comunidad de Madrid, Madrid, Spain.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Sanz
authors.forename: María Del Mar
authors.initials: Mdel M
authors.lastname: Mosquera
authors.forename: Juan Emilio
authors.initials: JE
authors.lastname: Echevarría
authors.forename: Marisa
authors.initials: M
authors.lastname: Fernández
authors.forename: Nieves
authors.initials: N
authors.lastname: Herranz
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
authors.forename: Fernando
authors.initials: F
authors.lastname: De Ory
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood; D009107:Mumps / Q000175:diagnosis* / Q000517:prevention & control; D009109:Mumps virus / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013183:Sputum / Q000821:virology; D014611:Vaccination
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M