title: [Influence of meliorated AS-PCR primer for ABO genotyping].
abstract: OBJECTIVE
  To improve the correct rate of ABO genotyping by meliorating AS-PCR primer.
  
  
  METHODS
  The primer P1 was changed into primer P1' by substituting the fifth base G for C of 3' end and the ABO genotyping results of primer P1 and P1' was compared and analysed.
  
  
  RESULTS
  The non-specific product of OO typing is reducing and the wrong genotyping of OO and AO was avoided by meliorating AS-PCR primer.
  
  
  CONCLUSIONS
  The rates of wrong ABO genotyping results could be effectively reduced by using altering primer P1'.
authors.affiliation: Weifang Public Security Bureau, Weifang 261061, China.
authors.forename: Xin-Jie
authors.initials: XJ
authors.lastname: Wang
authors.forename: Li-Jiang
authors.initials: LJ
authors.lastname: Diao
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Feng
authors.forename: Gui-Ping
authors.initials: GP
authors.lastname: Lu
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000017:ABO Blood-Group System; D017931:DNA Primers