The blood peptidome: a higher dimension of information content for cancer biomarker discovery.
The low-molecular-weight range of the circulatory proteome is termed the 'peptidome', and could be a rich source of cancer-specific diagnostic information because it is a 'recording' of the cellular and extracellular enzymatic events that take place at the level of the cancer-tissue microenvironment. This new information archive seems to mainly exist in vivo, bound to high-abundance proteins such as albumin. Measuring panels of peptidome markers might be more sensitive and specific than conventional biomarker approaches. We discuss the advantages and disadvantages of various methods for studying the peptidome.
Petricoin
Emanuel F
EF
Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas Virginia 20110, USA. epetrico@gmu.edu
Belluco
Claudio
C
Araujo
Robyn P
RP
Liotta
Lance A
LA
D015982:Bias; D014408:Biomarkers, Tumor / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D002352:Carrier Proteins / Q000097:blood; D002498:Centrifugation / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D007163:Immunosorbent Techniques; D007553:Isotope Labeling / Q000379:methods; D008970:Molecular Weight; D009369:Neoplasms / Q000097:blood* / Q000473:pathology; D010446:Peptide Fragments / Q000097:blood; D010455:Peptides / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D040081:Protein Array Analysis; D020543:Proteome; D040901:Proteomics / Q000379:methods / Q000639:trends; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D049490:Systems Biology
D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D010446:Peptide Fragments; D010455:Peptides; D020543:Proteome