Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding.
By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (approximately 1 cm2) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm2), and specific surface-based protein capture from a sample volume (100 microL) containing a low amount of protein (160 attomol in pure buffer and 400 attomol in serum). The design criteria for this surface modification were achieved using QCM-D (quartz crystal microbalance with energy dissipation monitoring) of serum protein adsorption onto PLL-g-PEG-modified oxidized PDMS. Equally good, or almost as good, results were obtained for oxidized SU-8, Topas, and poly(methyl metacrylate) (PMMA), demonstrating the generic potential of PLL-g-PEG for surface modification in various micro-fluidic applications.
Marie
Rodolphe
R
Division of Solid State Physics, Lund University, P.O. Box 118, SE-221 00, Lund, Sweden.
Beech
Jason P
JP
Vörös
Janos
J
Tegenfeldt
Jonas O
JO
Höök
Fredrik
F
D000327:Adsorption; D001710:Biotin / Q000737:chemistry; D003460:Crystallization; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D044085:Microfluidics / Q000379:methods*; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D019946:Propylene Glycol / Q000737:chemistry; D011485:Protein Binding; D012828:Silicones / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties
D004129:Dimethylpolysiloxanes; D011108:Polymers; D012828:Silicones; D011107:Polylysine; D011092:Polyethylene Glycols; C013830:baysilon; D019946:Propylene Glycol; D001710:Biotin; D019809:Streptavidin