Increased lipids in non-lipogenic tissues are indicators of the severity of type 2 diabetes in mice.
We hypothesised that the molecular changes triggered in type 2 diabetes might cause phenotypic changes in the lipid fraction of tissues. We compared tissue lipid profiles of inbred lean B6-Bom with those of the obese B6-ob/ob and diabetic BKS-db/db mice and found that genetically diabetic mice significantly accumulate fat (especially monounsaturated fatty acids, MUFA) in non-lipogenic tissues such as the eye (MUFA, 2-fold), skeletal muscle (MUFA, 13-fold) and pancreas (MUFA, 16-fold). In contrast, the B6-ob/ob mice which manifest a milder form of type 2 diabetes use the liver as their predominant lipid depot (MUFA 91-fold increase, as compared to lean mice values). The lipids in the BKS-db/db skeletal muscle and pancreas were also significantly enriched with linoleic acid (LA, (9-fold and 6-fold, respectively); and alpha-linolenic acid (ALA, 8.5-fold and 8-fold, respectively). MUFA, LA and ALA accumulation in the non-lipogenic tissues of BKS-db/db mice was associated with reduced liver stearoyl-CoA desaturase-1 expression.
Campbell-Tofte
Joan
J
Department of Medicinal Chemistry, Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK-2100 Copenhagen, Denmark. jct@dfuni.dk
Hansen
Harald S
HS
Mu
Huiling
H
Mølgaard
Per
P
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D015536:Down-Regulation; D005123:Eye / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005786:Gene Expression Regulation; D050356:Lipid Metabolism / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008820:Mice, Obese; D018482:Muscle, Skeletal / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
D015415:Biomarkers; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D014280:Triglycerides; D013230:Stearoyl-CoA Desaturase