Production of germline transgenic chickens expressing enhanced green fluorescent protein using a MoMLV-based retrovirus vector.
The Moloney murine leukemia virus (MoMLV) -based retrovirus vector system has been used most often in gene transfer work, but has been known to cause silencing of the imported gene in transgenic animals. In the present study, using a MoMLV-based retrovirus vector, we successfully generated a new transgenic chicken line expressing high levels of enhanced green fluorescent protein (eGFP). The level of eGFP expression was conserved after germline transmission and as much as 100 microg of eGFP could be detected per 1 mg of tissue protein. DNA sequencing showed that the transgene had been integrated at chromosome 26 of the G1 and G2 generation transgenic chickens. Owing to the stable integration of the transgene, it is now feasible to produce G3 generation of homozygous eGFP transgenic chickens that will provide 100% transgenic eggs. These results will help establish a useful transgenic chicken model system for studies of embryonic development and for efficient production of transgenic chickens as bioreactors.
Koo
Bon Chul
BC
Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, Korea.
Kwon
Mo Sun
MS
Choi
Bok Ryul
BR
Kim
Jin-Hoi
JH
Cho
Seong-Keun
SK
Sohn
Sea Hwan
SH
Cho
Eun Jung
EJ
Lee
Hoon Taek
HT
Chang
Wonkyung
W
Jeon
Iksoo
I
Park
Jin-Ki
JK
Park
Jae Bok
JB
Kim
Teoan
T
D000818:Animals; D030801:Animals, Genetically Modified; D002642:Chick Embryo; D002645:Chickens; D047108:Embryonic Development; D005822:Genetic Vectors; D018095:Germ-Line Mutation; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D023421:Models, Animal; D008979:Moloney murine leukemia virus / Q000235:genetics*; D011994:Recombinant Proteins / Q000378:metabolism
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins