title: [Study on antitumor activities of huanglian jiedu decoction].
abstract: OBJECTIVE
  To study the antitumor activity of Huanglian Jiedu decoction (HLJDT).
  
  
  METHODS
  Antitumor activities were tested in mice with experimental tumor H22 in vivo, and the thymus index, spleen index and tumor inhibitory rate were evaluated. The effects on cancer cells from human were investigated in vitro using serum pharmacological approach. Swille, SPC-A-1, SGC-7901 and MCF-7 cancer cells were incubated in culture media containing serum from mice medicated with HLJDT. The inhibitory effects of HLJDT serum were observed by MTT assay.
  
  
  RESULTS
  HLJDT showed significant antitumor activities on H22 in mice. All of the HLJDT serum in different dosage groups could highly inhibit the proliferation of 4 cancer cell lines from human.
  
  
  CONCLUSIONS
  The HLJDT can significantly inhibit the tumor H22 in mice in a dose-dependent manner, the drug serum has obvious anticancer effects against Swille, SPC-A-1, SGC-7901 and MCF-7.
authors.affiliation: School of Pharmacy, Jilin University, Changchun 130021, China. sunjiankw@163.com
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Qing-Hui
authors.initials: QH
authors.lastname: Wen
authors.forename: Yu
authors.initials: Y
authors.lastname: Song
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Jin
authors.forename: Ji-Sheng
authors.initials: JS
authors.lastname: Ma
authors.forename: Qiu-Li
authors.initials: QL
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D013950:Thymus Gland / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal