Immunomodulation produced by a green propolis extract on humoral and cellular responses of mice immunized with SuHV-1.
Despite recent technological advances in vaccine production, most the vaccines depend on the association with adjuvant substances. This work evaluated the adjuvant capacity of an ethanol extract of green propolis associated to inactivated Suid herpesvirus type 1 (SuHV-1) vaccine preparations. Mice inoculated with SuHV-1 vaccine plus aluminum hydroxide and 5mg/dose of propolis extract presented higher levels of antibodies when compared to animals that received the same vaccine without propolis. The use of SuHV-1 vaccine with propolis extract alone did not induce significant levels of antibodies, however it was able to increase the cellular immune response, evidenced by the increase in the expression of mRNA to IFN-gamma. Besides, propolis increased the percentage of protected animals against challenge with a lethal dose of SuHV-1. The effect of green propolis extract on the humoral and cellular immune responses may be exploited for the development of effective vaccines.
Fischer
Geferson
G
Centro de Biotecnologia, Laboratório de Virologia e Imunologia, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), CP 354, 96010-900 Pelotas, RS, Brazil. geferson.fischer@gmail.com
Conceição
Fabricio Rochedo
FR
Leite
Fábio Pereira Leivas
FP
Dummer
Luana Alves
LA
Vargas
Gilberto D'Avila
GD
Hübner
Sílvia de Oliveira
Sde O
Dellagostin
Odir Antônio
OA
Paulino
Niraldo
N
Paulino
Amarílis Scremin
AS
Vidor
Telmo
T
D000276:Adjuvants, Immunologic; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D004587:Electrophoresis, Agar Gel; D000431:Ethanol; D005260:Female; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007111:Immunity, Cellular / Q000187:drug effects*; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011429:Propolis / Q000494:pharmacology*; D011557:Pseudorabies / Q000276:immunology / Q000517:prevention & control; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012997:Solvents
D000276:Adjuvants, Immunologic; D012333:RNA, Messenger; D012997:Solvents; D000431:Ethanol; D007371:Interferon-gamma; D011429:Propolis