title: Levels of platelet activating factor in gingival crevice fluid following periodontal surgical therapy.
abstract: OBJECTIVE
  Elevated levels of platelet activating factor (PAF), a potent inflammatory phospholipid mediator, have been previously detected in gingival tissues and gingival crevice fluid (GCF) in periodontal disease. However, the role of this mediator during wound healing after periodontal surgery remains unclear. The hypothesis, a relationship between PAF levels and periodontal healing, was tested by measuring PAF levels in GCF samples collected from sites that had undergone guided tissue regeneration (GTR) or flap surgery.
  
  
  METHODS
  Using a split-mouth design, 30 intrabony defects were randomly assigned to treatment with GTR (group 1) or to flap surgery (group 2). GCF was sampled pre-operatively and at 6-, 12- and 24-wk follow-up evaluation visits. PAF levels in GCF were analyzed by high-performance liquid chromatography (HPLC).
  
  
  RESULTS
  Both treatment modalities significantly reduced the probing pocket depth and improved the clinical attachment level (p < 0.01). Compared with pre-operative values, the GCF volume and PAF levels were significantly decreased at postoperative weeks 6, 12 and 24 in both groups (p < 0.01). There were also significant differences in GCF volume and PAF levels at all time points up to 24 wks in both groups (p < 0.01). No statistically significant differences were observed in any of the parameters investigated between the two groups (p > 0.05).
  
  
  CONCLUSIONS
  PAF is detectable in GCF by HPLC and showed a continuous decrease at all the time points monitored following periodontal surgical therapy. This suggests that changes in the levels of this mediator in GCF might be useful for monitoring the progress of periodontal repair and regeneration.
authors.affiliation: Faculty of Dentistry, Department of Periodontology, Ondokuzmayis University, Samsun, Turkey.
authors.forename: G C
authors.initials: GC
authors.lastname: Keles
authors.forename: B O
authors.initials: BO
authors.lastname: Cetinkaya
authors.forename: I
authors.initials: I
authors.lastname: Isildak
authors.forename: H
authors.initials: H
authors.lastname: Koprulu
authors.forename: G
authors.initials: G
authors.lastname: Acikgoz
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid; D004812:Epidemiologic Methods; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D016557:Guided Tissue Regeneration, Periodontal / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010510:Periodontal Diseases / Q000601:surgery*; D010514:Periodontal Pocket / Q000601:surgery; D010972:Platelet Activating Factor / Q000032:analysis*; D013524:Surgical Flaps; D014945:Wound Healing
chemical_list: D010972:Platelet Activating Factor