title: Isolation and characterization of a novel bradykinin potentiating peptide (BPP) from the skin secretion of Phyllomedusa hypochondrialis.
abstract: Bradykinin potentiating peptides (BPPs) from Bothrops jararaca venom were first described in the middle of 1960s and were the first natural inhibitors of the angiotensin-converting enzyme (ACE). BPPs present a classical motif and can be recognized by their typical pyroglutamyl (Pyr)/proline rich sequences presenting, invariably, a proline residue at the C-terminus. In the present study, we describe the isolation and biological characterization of a novel BPP isolated from the skin secretion of the Brazilian tree-frog Phyllomedusa hypochondrialis. This new BPP, named Phypo Xa presents the sequence Pyr-Phe-Arg-Pro-Ser-Tyr-Gln-Ile-Pro-Pro and is able to potentiate bradykinin activities in vivo and in vitro, as well as efficiently and competitively inhibit ACE. This is the first canonical BPP (i.e. Pyr-Aaa(n)-Gln-Ile-Pro-Pro) to be found not only in the frog skin but also in any other natural source other than the snake venoms.
authors.affiliation: Laboratório Especial de Toxinologia Aplicada, Center for Applied Toxinology (CAT/CEPID), Instituto Butantan, Avenida Vital Brasil 1500, São Paulo, SP 05503-900, Brazil.
authors.forename: Katia
authors.initials: K
authors.lastname: Conceição
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Konno
authors.forename: Robson Lopes
authors.initials: RL
authors.lastname: de Melo
authors.forename: Marta M
authors.initials: MM
authors.lastname: Antoniazzi
authors.forename: Carlos
authors.initials: C
authors.lastname: Jared
authors.forename: Juliana M
authors.initials: JM
authors.lastname: Sciani
authors.forename: Isaltino M
authors.initials: IM
authors.lastname: Conceição
authors.forename: Benedito C
authors.initials: BC
authors.lastname: Prezoto
authors.forename: Antônio Carlos Martins
authors.initials: AC
authors.lastname: de Camargo
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Pimenta
mesh_terms: D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001001:Anura / Q000235:genetics / Q000378:metabolism*; D001794:Blood Pressure / Q000187:drug effects; D001920:Bradykinin / Q000378:metabolism* / Q000494:pharmacology; D004357:Drug Synergism; D005260:Female; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009842:Oligopeptides / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000378:metabolism
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D009842:Oligopeptides; C006791:bradykinin potentiating factors; D001920:Bradykinin