title: Catalase expression in pancreatic alpha cells of diabetic and non-diabetic mice.
abstract: The pancreatic islet beta cells are very sensitive to oxidative stress, probably due to the extremely low level of anti-oxidant enzymes, particularly catalase. In contrast to beta cells, pancreatic alpha cells are significantly more resistant to diabetogenic toxins. However, whether alpha cells express a different level of catalase is not known. The aim of this study was to evaluate catalase expression in alpha cells of diabetic and non-diabetic mice. Diabetes was induced by a single injection of streptozotocin. After 3 weeks of persistent hyperglycemia, pancreatic tissues were collected. Catalase localization in alpha cells was identified by a dual-immunofluorescence staining with anti-glucagon and anti-catalase antibodies. In intact mice, intensive catalase and glucagon immunostaining was found in the peripheral area of islets. Merged images of glucagon and catalase show their localization in the same cell type, namely, alpha cells. Confocal microscopy indicated that the glucagon and catalase staining was distributed throughout the cytoplasm. Similar co-expression of catalase and glucagon was found in the alpha cells of diabetic animals. The results of this study show the intensive catalase expression in alpha cells of diabetic and non-diabetic mice. This knowledge may be useful to better understand the defense mechanisms of pancreatic alpha cells against oxidative stress.
authors.affiliation: Diabetes and Obesity Research Laboratory, Felsenstein Medical Research Center, Sackler Faculty of Medicine, Tel-Aviv University, Petah Tikva, 49100, Israel. kbloch@post.tau.ac.il
authors.forename: Konstantin
authors.initials: K
authors.lastname: Bloch
authors.forename: Elina
authors.initials: E
authors.lastname: Shichman
authors.forename: Marina
authors.initials: M
authors.lastname: Vorobeychik
authors.forename: Daria
authors.initials: D
authors.lastname: Bloch
authors.forename: Pnina
authors.initials: P
authors.lastname: Vardi
mesh_terms: D000818:Animals; D002374:Catalase / Q000302:isolation & purification / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005934:Glucagon / Q000302:isolation & purification / Q000378:metabolism; D050416:Glucagon-Secreting Cells / Q000166:cytology / Q000201:enzymology*; D007150:Immunohistochemistry; D007328:Insulin / Q000302:isolation & purification / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D018384:Oxidative Stress; D013311:Streptozocin
chemical_list: D007328:Insulin; D013311:Streptozocin; D005934:Glucagon; D002374:Catalase