Increase of NKG2D ligands and sensitivity to NK cell-mediated cytotoxicity of tumor cells by heat shock and ionizing radiation.
In this study, we have investigated if current cancer therapeutic modalities including hyperthermia and ionizing radiation can increase the expression of NKG2D ligands in human cancer cell lines. The expressions of NKG2D ligands were induced by both heat shock and ionizing radiation in various cell lines including KM12, NCI-H23, HeLa and A375 cells with peaks at 2 h and 9 h after treatment, respectively, although inducibility of each NKG2D ligand was various depending on cell lines. During the induction of NKG2D ligands, heat shock protein 70 was induced by heat shock but not by ionizing radiation. These results were followed by increased susceptibilities to NK cell-mediated cytolysis after treatment with heat shock and ionizing radiation. These results suggest that heat shock and ionizing radiation induce NKG2D ligands and consequently might lead to increased NK cell-mediated cytotoxicity in various cancer cells.
Kim
Joo-Young
JY
Department of Biochemistry, Pusan National University School of Medicine, Busan 602-739, Korea.
Son
Young-Ok
YO
Park
Soon-Won
SW
Bae
Jae-Ho
JH
Chung
Joo Seop
JS
Kim
Hyung Hoi
HH
Chung
Byung-Seon
BS
Kim
Sun-Hee
SH
Kang
Chi-Dug
CD
D000954:Antigens, Surface / Q000378:metabolism / Q000528:radiation effects; D003602:Cytotoxicity, Immunologic / Q000502:physiology* / Q000528:radiation effects*; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism / Q000528:radiation effects; D006367:HeLa Cells; D018869:Heat-Shock Response / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods; D007694:Killer Cells, Natural / Q000276:immunology*; D008024:Ligands; D055655:NK Cell Lectin-Like Receptor Subfamily K; D009369:Neoplasms / Q000276:immunology / Q000532:radiotherapy* / Q000628:therapy; D011839:Radiation, Ionizing; D011971:Receptors, Immunologic / Q000378:metabolism*; D055607:Receptors, Natural Killer Cell; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D018840:HSP70 Heat-Shock Proteins; C527085:KLRK1 protein, human; D008024:Ligands; D055655:NK Cell Lectin-Like Receptor Subfamily K; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell