Pretreatment of hypertonic saline can increase endogenous interleukin 10 release to attenuate hepatic ischemia reperfusion injury.
Ischemia-reperfusion (I/R) injury of the liver occurs in many clinical cases. Many steps are associated with hepatic I/R injury, including the release of many inflammatory molecules and infiltration of neutrophils into the liver. Recent studies revealed that hypertonic saline (HTS) has a strong anti-inflammatory effect and can inhibit a varity of neutrophil functions. So pretreatment with HTS may attenuate the liver injury associated with I/R. In this study, rats were divided into three groups: the sham group (S group), hepatic I/R group (I/R group), and HTS pretreatment group (HTS group). Serum ALT and myeloperoxidase (MPO) activity were determined. Serum tumor necrosis factor alpha (TNF-alpha) and interleukin 10 (IL-10) were determined by enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction analysis was used to assess the mRNA expressions of TNF-alpha and IL-10. Protein expressions of TNF-alpha, IL-10, STAT3, and phosphorylated STAT3 were analyzed by Western blot. Results showed that HTS pretreatment can augment the release of endogenous IL-10 by activating STAT3 in the process of hepatic I/R injury. Serum ALT levels, MPO activity in liver, generation of TNF-alpha, and infiltration of neutrophils in liver were inhibited in the HTS group. So we concluded that HTS pretreatment attenuates hepatic I/R injury by increasing the release of endogenous IL-10.
Ke
Qing-Hong
QH
Department of Hepatobiliary and Pancreatic Surgery The First Affiliated Hospital, College of Medicine, Key Lab of Combined Muti-organ Transplantation, Ministry of Health, Zhejiang University, Hangzhou, 310003, P.R. China. keqinghong@zju.edu.cn
Zheng
Shu-Sen
SS
Liang
Ting-Bo
TB
Xie
Hai-Yang
HY
Xia
Wei-Liang
WL
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D016753:Interleukin-10 / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008107:Liver Diseases / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D050796:STAT3 Transcription Factor / Q000378:metabolism; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D050796:STAT3 Transcription Factor; D012462:Saline Solution, Hypertonic; C494088:Stat3 protein, rat; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009195:Peroxidase; D000410:Alanine Transaminase