Fatty acids reduce the tensile strength of fungal hyphae during cephalosporin C production in Acremonium chrysogenum.
Fragmentation rate constants, which can be used to estimate the tensile strength of fungal hyphae, were used to elucidate relationships between morphological changes and addition of fatty acids during cephalosporin C production in Acremonium chrysogenum M35. The number of arthrospores increased gradually during fermentation, and, in particular, was higher in the presence of rice oil, oleic acid or linoleic acid than in their absence. Because supplementation of rice oil or fatty acids increased cephalosporin C, we concluded that differentiation to arthrospores is related to cephalosporin C production. To estimate the relative tensile strengths of fungal hyphae, fragmentation rate constants (k (frag)) were measured. When rice oil, oleic acid, or linoleic acid were added into medium, fragmentation rate constants were higher than for the control, and hyphal tensile strengths reduced. The relative tensile strength of fungal hyphae, however was not constant presumably due to differences in physiological state.
Kim
Jong Chae
JC
Department of Chemical and Biological Engineering, Korea University, Seoul, Korea.
Song
Yoon Seok
YS
Lee
Dong Hwan
DH
Kang
Seong Woo
SW
Kim
Seung Wook
SW
D000164:Acremonium / Q000502:physiology* / Q000648:ultrastructure*; D002511:Cephalosporins / Q000378:metabolism*; D005227:Fatty Acids / Q000502:physiology*; D025301:Hyphae / Q000502:physiology*; D013718:Tensile Strength / Q000502:physiology
D002511:Cephalosporins; D005227:Fatty Acids; C025163:cephalosporin C