title: Immune enhancing effects of Lactobacillus acidophilus LAFTI L10 and Lactobacillus paracasei LAFTI L26 in mice.
abstract: The immune enhancing properties of Lactobacillus acidophilus LAFTI L10 and Lactobacillus paracasei LAFTI L26 in mice were investigated. Each mouse (BALB/c) was orally fed with cultures of either L. acidophilus or L. paracasei at 10(8) CFU/50 mul per day for 14 days. The effect of these strains on immunoglobulin A, interleukin-10 and interferon gamma producing cells in the gut immune system was determined by immunofluorescence assays. Systemic immune responses were analysed in mice serum upon euthanising after a 14-day feeding trial to estimate cytokines such as IL-10 and IFN-gamma by enzyme-linked immunosorbent assays. L. acidophilus and L. paracasei strains demonstrated an increase in the number of IgA producing cells, IL-10 and IFN-gamma cytokine producing cells in the small intestine. In the systemic immune response, mice fed with L. acidophilus or L. paracasei also enhanced the secretion of anti-inflammatory cytokine (IL-10) and pro-inflammatory cytokine (IFN-gamma). The results of this study suggest that L. acidophilus and L. paracasei were able to enhance specific gut and systemic immune responses in mice.
authors.affiliation: Centre for Plant and Food Science, University of Western Sydney, Hawkesbury Campus, Locked Bag 1797, Penrith South DC, NSW 1797, Australia.
authors.forename: Gunaranjan
authors.initials: G
authors.lastname: Paturi
authors.forename: Michael
authors.initials: M
authors.lastname: Phillips
authors.forename: Mark
authors.initials: M
authors.lastname: Jones
authors.forename: Kasipathy
authors.initials: K
authors.lastname: Kailasapathy
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D004797:Enzyme-Linked Immunosorbent Assay; D019085:Fluorescent Antibody Technique, Direct; D007070:Immunoglobulin A / Q000378:metabolism*; D007371:Interferon-gamma / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000096:biosynthesis*; D007421:Intestine, Small / Q000276:immunology* / Q000382:microbiology*; D007778:Lactobacillus / Q000276:immunology*; D007779:Lactobacillus acidophilus / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation
chemical_list: D007070:Immunoglobulin A; D016753:Interleukin-10; D007371:Interferon-gamma