Genotyping of Newcastle disease viruses isolated from 2002 to 2004 in China.
The main function region of the fusion (F) protein gene of 124 strains of Newcastle disease virus isolated from 2002 to 2004 in China was amplified and sequenced for further phylogenetic and residue substitutive analysis. Most of the isolates were classified into genotype VIIc, VIId, VIf, and VIb, while others into genotype IX, III, or II. The genotype IX, a unique genotype which includes strain F48, the first Chinese virulent NDV strain isolated in 1948, were still found inducing sporadic infections in certain areas. Subgenotype VIIc, VIId, and VIIe viruses, which were distributed in clusters in the phylogenetic tree distinct from members of subgenotypes VIIa and VIIb, were responsible for most outbreaks in China and circulated predominantly in China in recent years. Strain NDV03-026, an isolate of the genotype II which was normally lentogenic, was found carrying (112)RRQKRF(117) motif at the cleavage site of F protein as the virulent strain.
Wang
Zhiliang
Z
National Reference Laboratory for Newcastle Disease, Animal Quarantine Institute, Ministry of Agriculture, 369 Nanjing Road, Qingdao, P.C. 266032, People's Republic of China. zlwang111@yahoo.com.cn
Liu
Hualei
H
Xu
Jiangtao
J
Bao
Jingyue
J
Zheng
Dongxia
D
Sun
Chengying
C
Wei
Rong
R
Song
Cuiping
C
Chen
Jiming
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D002681:China; D016000:Cluster Analysis; D005838:Genotype; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D011200:Poultry; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms
D012367:RNA, Viral