Identification of homologous genes of T. annulata proteins in the genome of Theileria sp. (China).
Homologues to previously described Theileria (T.) annulata genes (T. annulata surface protein [TaSP], putative T. annulata membrane protein [TaD]) were successfully amplified by polymerase chain reaction (PCR) from Theileria sp. (China) merozoite cDNA, with 88% identity to TaD; TcSP partial cDNA, 94% identity to TaSP. Moreover, homologues to a secretory protein of T. annulata (TaSE), with a sequence identity of 99% on the cDNA level (TcSE partial cDNA) and to a potential membrane protein of T. lestoquardi (Clone-5), with a sequence identity of 100% on the genomic level (Tc Clone-5) but lacking an intron at positions 1894-1928 were identified.
Miranda
Joana P G
JP
Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.
Bakheit
Mohammed
M
Schneider
Ilka
I
Haller
Daniel
D
Ahmed
Jabbar S
JS
Yin
Hong
H
Oliva
Abel G
AG
Seitzer
Ulrike
U
D000818:Animals; D005784:Gene Amplification; D015723:Gene Library; D017125:Genes, Protozoan; D018503:Genome, Protozoan / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D015800:Protozoan Proteins / Q000235:genetics*; D016052:Protozoan Vaccines; D017386:Sequence Homology, Amino Acid; D016796:Theileria annulata / Q000235:genetics*; D013801:Theileriasis / Q000175:diagnosis* / Q000469:parasitology / Q000517:prevention & control
D008565:Membrane Proteins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C456259:TaSP protein, Theileria annulata