title: Bone-marrow-derived mesenchymal stem cells as a target for cytomegalovirus infection: implications for hematopoiesis, self-renewal and differentiation potential.
abstract: Mesenchymal stem cells (MSCs) in bone marrow (BM) regulate the differentiation and proliferation of adjacent hematopoietic precursor cells and contribute to the regeneration of mesenchymal tissues, including bone, cartilage, fat and connective tissue. BM is an important site for the pathogenesis of human cytomegalovirus (HCMV) where the virus establishes latency in hematopoietic progenitors and can transmit after reactivation to neighboring cells. Here we demonstrate that BM-MSCs are permissive to productive HCMV infection, and that HCMV alters the function of MSCs: (i) by changing the repertoire of cell surface molecules in BM-MSCs, HCMV modifies the pattern of interaction between BM-MSCs and hematopoietic cells; (ii) HCMV infection of BM-MSCs undergoing adipogenic or osteogenic differentiation impaired the process of differentiation. Our results suggest that by altering BM-MSC biology, HCMV may contribute to the development of various diseases.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07103, USA.
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Smirnov
authors.forename: Ryhor
authors.initials: R
authors.lastname: Harbacheuski
authors.forename: Anita
authors.initials: A
authors.lastname: Lewis-Antes
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Pranela
authors.initials: P
authors.lastname: Rameshwar
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Kotenko
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology / Q000821:virology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000472:pathogenicity / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000821:virology*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology / Q000821:virology*; D014774:Virulence
chemical_list: D008565:Membrane Proteins