Isolation of Anaplasma phagocytophilum strain Ap-variant 1 in a tick-derived cell line.
Ten isolates of the Ap-Variant 1 strain of Anaplasma phagocytophilum were made in the Ixodes scapularis (I. scapularis)-derived cell line, ISE6. Two isolates were obtained from laboratory-infected goats and eight isolates were obtained from field-collected I. scapularis ticks. Each isolate showed 16S rRNA sequences identical to those as previously described for the Ap-Variant 1 strain. These are the first tissue culture isolates of the Ap-Variant 1 strain and will allow for further characterization of the biological and antigenic properties of this strain.
Massung
Robert F
RF
Centers for Disease Control and Prevention, 1600 Clifton Rd., MS G-13, Atlanta, GA 30333, USA. rfm2@cdc.gov
Levin
Michael L
ML
Munderloh
Ulrike G
UG
Silverman
David J
DJ
Lynch
Meghan J
MJ
Kurtti
Timothy J
TJ
D041081:Anaplasma phagocytophilum / Q000235:genetics* / Q000302:isolation & purification*; D000818:Animals; D002460:Cell Line; D016873:Ehrlichiosis / Q000382:microbiology / Q000473:pathology; D014644:Genetic Variation; D006041:Goats / Q000382:microbiology; D006801:Humans; D013987:Ticks / Q000382:microbiology*