[The analysis of somatic antigens extracted from Aspiculuris tetraptera (Oxyuridae) and their role in eliciting immune response in laboratory mice].
BACKGROUND
The aim of this study was isolation and examination of Aspiculuris tetraptera somatic proteins and somatic antigens role in eliciting of immune response in laboratory mice.


METHODS
In our investigation 40 laboratory mice (BALB/c strain) were used. To extract the somatic proteins Tris-HCl buffer with NaCl and Triton X-100 was used. The analysis of somatic antigens was undertaken by Western blotting.


RESULTS
The study showed the presence of 14 protein bands ranging from approximately 82 to 28 kDa. Glicoproteins detection revealed 13 bands in range between approximately 70 to 30 kDa. There was no reaction observed with immunoglobulins IgA. Comparision of these results with earlier studies concerning S. obvelata somatic antigens show that there are proteins and glicoproteins with the same molecular weights for both species. It is also observed that S. obvelata somatic extract is more diversed and have higher antigenicity than A. tetraptera. Hence, we may suppose this fact could favour easier colonization of the host by A. tetraptera.
Zaleśny
Grzegorz
G
Zakład Parazytologii, Instytut Genetyki i Mikrobiologii, Uniwersytet Wrocławski, ul. Przybyszewskiego 63/77, 51-148 Wrocław. zalesny@microb.uni.wroc.pl
Perec-Matysiak
Agnieszka
A
Okulewicz
Anna
A
D000818:Animals; D000830:Animals, Laboratory / Q000276:immunology* / Q000469:parasitology*; D000906:Antibodies / Q000032:analysis / Q000276:immunology*; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D051379:Mice; D010123:Oxyuriasis / Q000662:veterinary*; D010124:Oxyuroidea / Q000737:chemistry* / Q000302:isolation & purification
D000906:Antibodies; D000941:Antigens