title: Mycotoxins and other secondary metabolites produced in vitro by Penicillium paneum Frisvad and Penicillium roqueforti Thom isolated from baled grass silage in Ireland.
abstract: Secondary metabolites produced by Penicillium paneum and Penicillium roqueforti from baled grass silage were analyzed. A total of 157 isolates were investigated, comprising 78 P. paneum and 79 P. roqueforti isolates randomly selected from more than 900 colonies cultured from bales. The findings mostly agreed with the literature, although some metabolites were not consistently produced by either fungus. Roquefortine C, marcfortine A, and andrastin A were consistently produced, whereas PR toxin and patulin were not. Five silage samples were screened for fungal metabolites, with two visually moldy samples containing up to 20 mg/kg of roquefortine C, mycophenolic acid, and andrastin A along with minor quantities (0.1-5 mg/kg) of roquefortines A, B, and D, festuclavine, marcfortine A, and agroclavine. Three visually nonmoldy samples contained low amounts of mycophenolic acid and andrastin A. The ability of both molds to produce a diverse range of secondary metabolites in vitro and in silage should be a concern to livestock producers.
authors.affiliation: Teagasc, Grange Beef Research Centre, Dunsany, County Meath, Ireland. martin.obrien@teagasc.ie
authors.forename: Martin
authors.initials: M
authors.lastname: O'Brien
authors.forename: Kristian F
authors.initials: KF
authors.lastname: Nielsen
authors.forename: Padraig
authors.initials: P
authors.lastname: O'Kiely
authors.forename: Patrick D
authors.initials: PD
authors.lastname: Forristal
authors.forename: Hubert T
authors.initials: HT
authors.lastname: Fuller
authors.forename: Jens C
authors.initials: JC
authors.lastname: Frisvad
mesh_terms: D006801:Humans; D066298:In Vitro Techniques; D007494:Ireland; D009183:Mycotoxins / Q000096:biosynthesis*; D010407:Penicillium / Q000145:classification / Q000378:metabolism*; D006109:Poaceae / Q000382:microbiology*; D012820:Silage / Q000382:microbiology
chemical_list: D009183:Mycotoxins