The subcellular localisation of trypanosome RRP6 and its association with the exosome.
The exosome, a complex of 3'-exoribonucleases and associated proteins, is involved in the degradation of eukaryotic mRNAs in the cytoplasm, and has RNA processing and quality control functions in the nucleus. In yeast, the nuclear exosome differs from the cytoplasmic one in that it contains an additional non-essential component, Rrp6p. In contrast, a small proportion of human RRP6 has been shown to localise to the cytoplasm as well. When we purified the Trypanosoma brucei exosome from cytosolic extracts we found RRP6, apparently in stoichiometric amounts. We here confirm that RRP6 is in the trypanosome cytoplasm and nucleus. The level of RRP6 was unaffected by depletion of core exosome subunits by RNA interference and over-expression of tagged RRP6 was possible, indicating that RRP6 can be present independent of exosome association.
Haile
Simon
S
ZMBH, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany.
Cristodero
Marina
M
Clayton
Christine
C
Estévez
Antonio M
AM
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism*; D007425:Intracellular Membranes / Q000201:enzymology*; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D034622:RNA Interference; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000235:genetics
D005095:Exoribonucleases