Formation of DHP-derived DNA adducts in vivo from dietary supplements and chinese herbal plant extracts containing carcinogenic pyrrolizidine alkaloids.
We recently determined that the metabolism of a series of tumorigenic pyrrolizidine alkaloids resulted in the formation of a set of 6,7-dihydro-7-hydroxy-1-hydroxymethyl-5H-pyrrolizine (DHP)-derived DNA adducts. These DHP-derived DNA adducts have been proposed as potential biomarkers of pyrrolizidine alkaloid tumorigenicity, as well as pyrrolizidine alkaloid exposure. In this paper, we report that DHP-derived DNA adducts are formed in the liver of female F344 rats, gavaged with three dietary supplements (comfrey root extract, comfrey compound oil, and coltsfoot root extract), or an extract of a Chinese herbal plant, flos farfara (Kuan Tong Hua).
Chou
Ming W
MW
Division of Biochemical Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, USA. mchou@nctr.fda.gov
Fu
Peter P
PP
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D018736:DNA Adducts / Q000378:metabolism*; D019587:Dietary Supplements / Q000633:toxicity*; D004095:Dihydropyridines / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000633:toxicity*; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D011763:Pyrrolizidine Alkaloids / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344
D018736:DNA Adducts; D004095:Dihydropyridines; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011763:Pyrrolizidine Alkaloids