title: Synthesis of glyco(lipo)peptides by liposome-mediated native chemical ligation.
abstract: Although native chemical ligation (NCL) is emerging as a powerful method for the assembly of (glyco)peptide building blocks, its applicability is reduced when peptide segments are poorly soluble in aqueous buffer. We have found that incorporating reactants in liposomes allows NCL of lipophilic peptides and lipopeptides. Furthermore, the reaction rates of liposome-mediated NCL are higher than traditional reaction conditions resulting in improved yields. [reaction: see text]
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
authors.forename: Sampat
authors.initials: S
authors.lastname: Ingale
authors.forename: Therese
authors.initials: T
authors.lastname: Buskas
authors.forename: Geert-Jan
authors.initials: GJ
authors.lastname: Boons
mesh_terms: D000595:Amino Acid Sequence; D002021:Buffers; D019496:Cancer Vaccines / Q000737:chemistry; D000939:Epitopes; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D007202:Indicators and Reagents; D008081:Liposomes / Q000737:chemistry*; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000737:chemistry; D012995:Solubility
chemical_list: D002021:Buffers; D019496:Cancer Vaccines; D000939:Epitopes; D006020:Glycopeptides; D007202:Indicators and Reagents; D008081:Liposomes; D018396:Mucin-1