title: Viral load in 1-day-old and 6-week-old chickens infected with chicken anaemia virus by the intraocular route.
abstract: Although the effects of chicken anaemia virus (CAV) infection have frequently been investigated in young chickens, there have been few studies of the pathogenesis of CAV infection in older birds. The aim of the work reported here was to study viral loads in 6-week-old chickens and to compare these with those seen in younger birds. Specific pathogen free chickens were inoculated at 1 day or at 6 weeks of age with 10(4) median tissue culture infective doses of CAV by the intraocular route. Chicks infected when 1 day old were euthanized at day 14, 18 or 22 post inoculation (p.i.), and those infected when 6 weeks old at day 16, 18 or 20 p.i. Their body and thymus weights were determined and samples were collected from their spleen, liver and thymus. A quantitative polymerase chain reaction assay was developed and used to determine the number of viral genome copies in the tissue samples. In both age groups, viral genome concentrations increased in all organs up to day 18 p.i. and reached a peak in the spleen and liver at day 18 p.i. The peak viral concentrations in the thymus were detected at day 18 in the younger birds and at day 20 p.i. in older chickens. These studies have shown that exposure to CAV in older birds leads to similar levels of active viral replication to those seen in younger birds, and may result in subclinical infections in older birds with the potential to increase susceptibility to other infectious agents.
authors.affiliation: Department of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia. a.kaffashi@pgrad.unimelb.edu.au
authors.forename: Amir
authors.initials: A
authors.lastname: Kaffashi
authors.forename: Amir H
authors.initials: AH
authors.lastname: Noormohammadi
authors.forename: Melinda L
authors.initials: ML
authors.lastname: Allott
authors.forename: Glenn F
authors.initials: GF
authors.lastname: Browning
mesh_terms: D000375:Aging; D000818:Animals; D001835:Body Weight; D017727:Chicken anemia virus / Q000302:isolation & purification*; D002645:Chickens; D018173:Circoviridae Infections / Q000097:blood* / Q000821:virology*; D008099:Liver / Q000821:virology; D013154:Spleen / Q000821:virology; D013950:Thymus Gland / Q000821:virology; D019562:Viral Load / Q000662:veterinary*