Inhibition of HIV-1 replication by an improved hairpin ribozyme that includes an RNA decoy.
An anti-Tat hairpin ribozyme and a TAR RNA decoy were combined in one molecule. The chimeric molecule strongly inhibited HIV-1 replication (measured as changes in p24 levels in viral replication assays). The inhibitory action of the ribodecozyme (85%) was significantly greater than that shown by ribozyme and a non-catalytic variant carrying the functional decoy RNA domain (55% and 35%, respectively). This represents a significant improvement of the inhibitory efficiency of the ribozyme, suggesting there is an additive inhibitory effect on HIV-1 replication by the catalytic and decoy domains. This strategy could be used to create new inhibitor RNAs with enhanced in vivo performance.
Barroso-DelJesus
Alicia
A
Instituto de Parasitología y Biomedicina López-Neyra, CSIC, Parque Tecnológico de Ciencias de la Salud, Avda, del Conocimiento s/n, Granada, Spain.
Puerta-Fernández
Elena
E
Tapia
Natalia
N
Romero-López
Cristina
C
Sánchez-Luque
Francisco José
FJ
Martínez
Miguel Angel
MA
Berzal-Herranz
Alfredo
A
D001483:Base Sequence; D002478:Cells, Cultured; D015195:Drug Design; D015696:Gene Products, tat / Q000037:antagonists & inhibitors; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D016337:RNA, Catalytic / Q000494:pharmacology*; D012367:RNA, Viral / Q000037:antagonists & inhibitors*; D014779:Virus Replication / Q000187:drug effects*; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D016337:RNA, Catalytic; D012367:RNA, Viral; C418481:hairpin ribozyme; D054322:tat Gene Products, Human Immunodeficiency Virus