title: Development of a novel luminol chemiluminescent method catalyzed by gold nanoparticles for determination of estrogens.
abstract: It has been found that gold nanoparticles (nano-Au) enhance the chemiluminescence (CL) of the luminol-hydrogen peroxide system and that estrogens inhibit these CL signals in alkaline solution. CL spectra, UV-visible spectra, X-ray photoelectron spectra (XPS), and transmission electron microscopy (TEM) were used to investigate the mechanism of the CL enhancement. On the basis of the inhibition, a flow-injection CL method has been established for determination of three natural estrogens. Under the optimized conditions, the linear range for determination of the estrogens was 0.07 to 7.0 micromol L(-1) for estrone, 0.04 to 10 micromol L(-1) for estradiol, and 0.1 to 10 micromol L(-1) for estriol. The detection limits were 3.2 nmol L(-1) for estrone, 7.7 nmol L(-1) for estradiol, and 49 nmol L(-1) for estriol, with RSD of 2.9, 2.6, and 1.8%, respectively. This method has been used for analysis of estrogens in commercial tablets and in urine samples from pregnant women.
authors.affiliation: Anhui Key Laboratory of Functional Molecular Solids, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000 Anhui, China. liyx72@yahoo.com
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.forename: Po
authors.initials: P
authors.lastname: Wang
authors.forename: Lun
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D002384:Catalysis; D004967:Estrogens / Q000032:analysis* / Q000652:urine; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol; D053768:Metal Nanoparticles; D008722:Methods; D011247:Pregnancy; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis; D013607:Tablets / Q000032:analysis
chemical_list: D004967:Estrogens; D013607:Tablets; D008165:Luminol; D006046:Gold