Acrosome reaction of mouse epididymal sperm on oocyte zona pellucida.
To improve assessment of the acrosome reaction of mouse epididymal sperm, we employed anti-Izumo1 antibody instead of antibodies against acrosomal proteins. The acrosomal states among acrosome-intact, spontaneously acrosome-reacted, truly acrosome-reacted, and probably dead and/or membrane-damaged sperm were clearly distinguished by combined application of anti-Izumo1 antibody, DNA dye Hoechst 33342, and monoclonal antibody MN7 to paraformaldehyde-fixed sperm. When the acrosome reaction of capacitated epididymal sperm on the oocyte zona pellucida was examined using anti-Izumo1 antibody, approximately 20% of sperm bound onto the zona pellucida were acrosome-reacted 30 min after insemination. We also observed the moment of the acrosome reaction of live sperm on the zona pellucida by time-lapse monitoring using fluorescein isothiocyanate-conjugated anti-Izumo1 antibody.
Yamashita
Misuzu
M
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
Yamagata
Kazuo
K
Tsumura
Keiko
K
Nakanishi
Tomoko
T
Baba
Tadashi
T
D020101:Acrosome Reaction / Q000187:drug effects; D000818:Animals; D000941:Antigens / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D004822:Epididymis / Q000166:cytology*; D005260:Female; D007120:Immunochemistry / Q000379:methods; D007136:Immunoglobulins / Q000276:immunology / Q000378:metabolism; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000187:drug effects; D015044:Zona Pellucida / Q000502:physiology*
D000941:Antigens; D007136:Immunoglobulins; D007476:Ionophores; C498177:Izumo1 protein, mouse; D008565:Membrane Proteins; C403409:acrin 1; D000001:Calcimycin