title: Impact of surface energy and roughness on cell distribution and viability.
abstract: The aim of this study was to assess the respective impacts of the surface energy and surface roughness of bare and coated steels on biofouling and sanitisation. Bioadhesion of Staphylococcus aureus CIP 53.154 was studied on two stainless steel surfaces with smooth or specific micro-topography. Two coatings were also studied: silicon oxide (hydrophilic) and polysiloxane (hydrophobic). On smooth surfaces, adhesion was reduced on an apolar coating and cell viability increased with the surface polarity. A specific micro-topography decreased the level of bacterial adhesion on bare surfaces by a factor ten. On this surface, only single adherent cells were observed, contrasting with cells in clusters on smoother surfaces. As a consequence, cell repartition influenced bacterial viability. Most isolated adherent cells were dead whereas cells in clusters were still alive. In addition, the quaternary ammonium chloride used in sanitisation, acted at once both as a tensio-active molecule and a biocide. It only displaced adherent cells but did not remove them.
authors.affiliation: Arcelor Group, Ugine & Alz Research Center, Isbergues, France. audrey.allion@bio-rad.com
authors.forename: Audrey
authors.initials: A
authors.lastname: Allion
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Baron
authors.forename: Laurence
authors.initials: L
authors.lastname: Boulange-Petermann
mesh_terms: D001422:Bacterial Adhesion; D015169:Colony Count, Microbial; D004202:Disinfectants / Q000494:pharmacology; D004203:Disinfection / Q000379:methods; D004866:Equipment Contamination; D006801:Humans; D050296:Microbial Viability; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D013193:Stainless Steel / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000254:growth & development* / Q000502:physiology*; D013499:Surface Properties
chemical_list: D004202:Disinfectants; D000644:Quaternary Ammonium Compounds; D013193:Stainless Steel