[Galanin increases the survival rate of hippocampal cells injured by H2O2 in vitro].
The method of primary hippocampal nerve cell culture was used to study the injury effect of H2O2 and the protective effect of galanin (GAL) and GAL receptor agonists. Result demonstrated that H2O2 has obvious dose relative toxicity to hippocampal cells in vitro. GAL and GAL's nonselective agonist GAL1-11, GalR2's selective agonist GAL2-11 can increase the survival rate of hippocampal cells suffered form H2O2. All the protective effects can be blocked by nonselective antagonist M35. The result indicates that GAL can protect hippocampal cells from oxidative injury in vitro, which is most probably mediated by GalR2.
Zhang
Ye
Y
School of Life Science of East China Normal University, Shanghai 200062.
Shan
Liang
L
Lou
Xiang Xin
XX
Yuan
Chong Gang
CG
D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D019004:Galanin / Q000235:genetics / Q000494:pharmacology* / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006624:Hippocampus / Q000166:cytology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007150:Immunohistochemistry; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D044092:Receptor, Galanin, Type 2 / Q000819:agonists / Q000235:genetics / Q000502:physiology; D044088:Receptors, Galanin / Q000819:agonists / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005904:Glial Fibrillary Acidic Protein; D044092:Receptor, Galanin, Type 2; D044088:Receptors, Galanin; D019004:Galanin; D006861:Hydrogen Peroxide