title: Secretory granules of hypophyseal and pancreatic endocrine cells contain proteins of the neuronal postsynaptic density.
abstract: The PDZ domain-containing protein Shank is a master scaffolding protein of the neuronal postsynaptic density and directly or indirectly links neurotransmitter receptors and cell adhesion molecules to the actin-based cytoskeleton. ProSAP/Shank proteins have recently also been detected in several non-neuronal cells in which they are mostly concentrated in the apical subplasmalemmal cytoplasm. In contrast, we have previously reported a more widespread cytoplasmic immunostaining pattern for the ProSAP1/Shank2 protein in endocrine cells at the light-microscopic level. Therefore, in the present study, we have determined the ultrastructural localization of ProSAP1/Shank2 and the ProSAP/Shank-interacting proteins ProSAPiP1 and IRSp53 in pancreatic islet and adenohypophyseal cells by using immunogold staining techniques. Dense immunolabeling of secretory granules including the granule core in cells such as hypophyseal somatotrophs and pancreatic B-cells indicates the unexpected presence of ProSAP/Shank and ProSAP/Shank-interacting proteins in the hormone-storing compartment of endocrine cells. Thus, ProSAP/Shank and certain ProSAP/Shank-interacting proteins exhibit distinct subcellular localizations in the different cell types, raising the possibility that the function of ProSAP/Shank proteins is more diverse than has been envisaged to date.
authors.affiliation: Department of Cell Biology, Centre of Anatomy, Hannover Medical School, 30625 Hannover, Germany. redecker.peter@mh-hannover.de
authors.forename: Peter
authors.initials: P
authors.lastname: Redecker
authors.forename: Jürgen
authors.initials: J
authors.lastname: Bockmann
authors.forename: Tobias M
authors.initials: TM
authors.lastname: Böckers
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D005260:Female; D007515:Islets of Langerhans / Q000166:cytology*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons; D010902:Pituitary Gland / Q000166:cytology*; D021381:Protein Transport; D051381:Rats; D017208:Rats, Wistar; D022142:Secretory Vesicles / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C503229:Baiap2 protein, rat; D002352:Carrier Proteins; C523118:Lzts3 protein, rat; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C485686:Shank2 protein, rat; C033652:postsynaptic density proteins