Calcineurin interacts with KIN-29, a Ser/Thr kinase, in Caenorhabditis elegans.
Calcineurin is a Ca2+/Calmodulin activated Ser/Thr phosphatase that is well conserved from yeast to human. In Caenorhabditis elegans, tax-6 and cnb-1 encode catalytic and regulatory subunits of calcineurin, respectively. We performed yeast two-hybrid screening using TAX-6 as a bait to identify calcineurin interacting proteins. KIN-29 is one of proteins that specifically interacted with TAX-6. KIN-29 is a Ser/Thr kinase previously shown to be involved in regulating gene expression of a subset of chemoreceptors in specific neurons. Both TAX-6 and KIN-29 are expressed in hypodermis, muscles, and neurons. Moreover, both calcineurin and kin-29 mutants exhibit similar phenotypes, namely small body size, small brood size, and slow growth. Here we describe specific genetic interaction between tax-6 and kin-29 in regulating body size, serotonin mediated egg laying, and chemoreceptor expression.
Singaravelu
Gunasekaran
G
Department of Life Science, GIST, 1 Oryong-Dong, Buk-Gu, Gwangju 500-712, Republic of Korea.
Song
Hyun-Ok
HO
Ji
Yon Ju
YJ
Jee
Changhoon
C
Park
Byung-Jae
BJ
Ahnn
Joohong
J
D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins; D019703:Calcineurin / Q000378:metabolism*; D002384:Catalysis; D018507:Gene Expression Regulation, Developmental; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D010063:Ovum / Q000187:drug effects; D010641:Phenotype; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D018035:Receptors, Odorant / Q000378:metabolism; D012701:Serotonin / Q000494:pharmacology
D029742:Caenorhabditis elegans Proteins; D021122:Protein Subunits; D018035:Receptors, Odorant; C514941:Str-2 protein, C elegans; D012701:Serotonin; C448260:kin-29 protein, C elegans; D017346:Protein Serine-Threonine Kinases; D019703:Calcineurin