Genetic and antigenic typing of border disease virus isolates in sheep from the Iberian Peninsula.
A selection of 10 pestiviruses isolated from sheep from the Iberian Peninsula from 2001 to 2004 was characterised at the molecular level. The 5' untranslated region (5'-UTR) and N(pro)-coding gene were amplified by the reverse transcription-polymerase chain reaction (RT-PCR) and sequenced directly from purified products. All isolates were also typed antigenically with a panel of monoclonal antibodies (mAbs) raised against representative isolates of the four recognised pestivirus species. The genetic typing placed all the isolates in a new tentative type 4 of border disease virus (BDV), which was closely related to a pestivirus recently found in Pyrenean chamois (Rupicapra pyrenaica pyrenaica). Overall, the genotyping indicated a relatively wide diversity of the BDV type 4, which was best defined on the basis of N(pro) sequences. Antigenically, the isolates were recognised by two pan-pestivirus specific anti-NS3 mAbs, but only by some of the anti-glycoprotein specific mAbs raised against BDV, indicating partial antigenic overlap with other BDV isolates.
Valdazo-González
B
B
Departamento de Patología Animal, Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24071 León, Spain.
Alvarez-Martínez
M
M
Sandvik
T
T
D020121:5' Untranslated Regions; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D001882:Border Disease / Q000821:virology*; D018083:Border disease virus / Q000145:classification* / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012756:Sheep
D020121:5' Untranslated Regions; D000911:Antibodies, Monoclonal