A method for discriminating a Japanese chicken, the Nagoya breed, using microsatellite markers.
The Nagoya breed native to Japan is popular as a dual-purpose breed for eggs and meat. The current study describes a method to discriminate between the Nagoya breed and other breeds and commercial stocks of chicken. Four strains of the Nagoya breed established at the Aichi-ken Agricultural Research Center were analyzed using 25 microsatellite markers. In these strains, 5 of the markers (ABR0015, ABR0257, ABR0417, ABR0495, and ADL0262) had a single allele. Other chicken samples (448) of various breeds and hybrids were analyzed using the same 5 markers. None of these chicken samples had the same allele combination as the Nagoya breed strains. These 5 microsatellite markers provide a practical method to accurately discriminate the Nagoya breed from other chicken breeds.
Nakamura
A
A
Poultry Laboratory, Animal Husbandry Research Division, Aichi-ken Agricultural Research Center, Sagamine, Yazako, Nagakute, Aichi 480-1193, Japan.
Kino
K
K
Minezawa
M
M
Noda
K
K
Takahashi
H
H
D000818:Animals; D001947:Breeding; D002645:Chickens / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D007564:Japan; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*
D004247:DNA