title: [Molecular identification of Ca2+ channels in human ejaculated sperm].
abstract: OBJECTIVE
  To identify the types of voltage-dependent Ca2+ channels (VDCCs) in human ejaculated sperm using molecular technology.
  
  
  METHODS
  Based on the WHO criteria, the donated semen was screened by computer-aided sperm analysis (CASA), the sperm optimized by swim-up method, and the VDCCs' alpha1 subunits examined by reverse transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Such mRNA messages as alpha1H, alpha1G, alpha1E, alpha1B and alpha1C were detected, but alpha1A and alpha1D were not.
  
  
  CONCLUSIONS
  Human motile sperms express multiple VDCC RNAs, among which T-type and non-L-type channel messages are likely to be predominantly expressed. Not only T-type but also non-L-type calcium channels may be major gates for the external calcium influx, which helps regulate sperm motility and acrosome reaction.
authors.affiliation: Department of Urology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science Technology, Wuhan, Hubei 430030, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Liu
authors.forename: Jia-Gui
authors.initials: JG
authors.lastname: Li
authors.forename: Zhang-Qun
authors.initials: ZQ
authors.lastname: Ye
authors.forename: Xiu-Lai
authors.initials: XL
authors.lastname: Wang
mesh_terms: D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000096:biosynthesis / Q000145:classification* / Q000235:genetics; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D012333:RNA, Messenger; D002118:Calcium