[Genetic variation in the 5' flanking region of bovine TLR4 gene and correlation with mastitis].
Toll-like receptor 4 recognizes pathogen ligands and mediates signaling to initiate innate and adaptive immune responses. In this experiment, a 477 bp segment of the 5'-flanking region of TLR4 gene of Chinese Holstein, Sanhe cattle and Chinese simmental was amplified by polymerase chain reaction. After sequencing, a polymorphic site in amplified production of TLR4 was identified of having either a G or a C at position 245. This polymorphism in the three populations was detected by digesting the fragment with restriction endonuclease Msp I. Results showed that both alleles (A and B) were found in the three populations and the value of polymorphism information content indicated that this was a moderate polymorphism. Chi2 test indicated that the polymorphism locus in Sanhe cattle did not fit Hardy-Weinberg equilibrium (P< 0.05). In addition, the effect of the TLR4 polymorphism on somatic cell score was analyzed, and the results indicated that the somatic cell score were significantly affected by lactation month and the type of breeds(P<0.05), but not by different genotypes (P > 0.05).
Wang
Xing-Ping
XP
Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100094, China. nwsuafwxp@163.com
Xu
Shang-Zhong
SZ
Ma
Teng-He
TH
Gao
Xue
X
Ren
Hong-Yan
HY
Chen
Jin-Bao
JB
D024506:5' Flanking Region / Q000235:genetics*; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D006579:Heterozygote; D007774:Lactation; D008414:Mastitis, Bovine / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000378:metabolism
D051197:Toll-Like Receptor 4; D004262:DNA Restriction Enzymes