High prevalence of HGV coinfection with HBV or HCV among northeastern Thai blood donors.
Hepatitis G viral (HGV) infection among northeastern Thai blood donors was determined by the nested RT-PCR technique. HGV RNA was amplified by the degenerated helicase primers for a product of the expected size of 83 base pairs were used in this study. Serum samples from 322 of three different categories of northeastern Thai blood donors were included in this study. There were 104 HBsAg and Anti-HCV seronegative blood donors (control group), 100 samples of HBs Ag seropositive blood donors (HBV infected group) and 118 serum samples from anti-HCV seropositive blood donors (HCV infected group). The results demonstrated that HGV RNA was not detected in the control group but was found in 10 individuals (10%) in the HBV infected group and 13 (11%) in the anti-HCV positive blood donors. The prevalences of HGV in both seropositive groups were significantly different from the control group (p = 0.001). HGV co-infection is highly prevalent among northeastern Thai blood donors who are infected with HBV or HCV. The results also reveal that blood donors seronegative for HCV and HBV are a low risk group for HGV infection.
Barusruk
Sahapat
S
Department of Clinical Immunology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand. sahapat@kku.ac.th
Urwijitaroon
Yupa
Y
D001782:Blood Donors; D018178:Flaviviridae Infections / Q000097:blood / Q000150:complications / Q000453:epidemiology*; D029407:GB virus C / Q000302:isolation & purification*; D005784:Gene Amplification; D016174:Hepacivirus / Q000302:isolation & purification; D006509:Hepatitis B / Q000097:blood / Q000150:complications / Q000453:epidemiology*; D006515:Hepatitis B virus / Q000302:isolation & purification; D006526:Hepatitis C / Q000097:blood / Q000150:complications / Q000453:epidemiology*; D006525:Hepatitis, Viral, Human / Q000097:blood / Q000150:complications / Q000453:epidemiology*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013785:Thailand / Q000453:epidemiology
D012367:RNA, Viral