title: [Radiation-inducible promoters-mediated cdglytk gene in the treatment of buccal carcinoma in golden hamster].
abstract: OBJECTIVE
  To observe the therapeutic effect of CDglyTK gene mediated by radiation-inducible promoters in the treatment of buccal carcinoma in Golden Hamster.
  
  
  METHODS
  Animal models of buccal carcinoma in golden hamster were established by painting 0.5% dimethyl-benzanthracene. The plasmids pcDNA (+) 3.1/E-CDglyTK were transfected into tumors by lipofectamine. 24 h later, the tumors were exposed to 3 Gy irradiation. Animals were monitored at regular intervals for volume of tumors. CDglyTK mRNA was assayed by RT-PCR. Apoptosis and proliferating cell nuclear antigen were detected respectively by in situ end-labeling and immunohistochemical methods.
  
  
  RESULTS
  Compared with control groups, the tumor was suppressed obviously by CDglyTK gene therapy combined with 3 Gy induction radiation. The expression of CDglyTK gene could be detected by RT-PCR in the transfected tumor, and up-regulation of CDglyTK expression was found in tumor exposed to radiation (P < 0.05). There was significant difference in apoptosis index or proliferation index between tumor without irradiation and tumor with irradiation (P < 0.05).
  
  
  CONCLUSIONS
  The radiation-inducible promoter can be served as a molecular switch to regulate the expression of CDglyTK gene in buccal carcinoma in golden hamster, and low dose induction radiation can significantly improve the therapeutic effects.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Yu
authors.forename: Hong-zhang
authors.initials: HZ
authors.lastname: Huang
authors.forename: Xiao-wen
authors.initials: XW
authors.lastname: Hu
authors.forename: Xi-qiang
authors.initials: XQ
authors.lastname: Liu
authors.forename: Hai-kuo
authors.initials: HK
authors.lastname: Tang
authors.forename: An-xun
authors.initials: AX
authors.lastname: Wang
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging / Q000628:therapy*; D002610:Cheek / Q000000981:diagnostic imaging*; D006224:Cricetinae; D043525:Cytosine Deaminase / Q000235:genetics; D041722:Genes, Transgenic, Suicide / Q000235:genetics*; D015316:Genetic Therapy / Q000379:methods*; D008647:Mesocricetus; D009062:Mouth Neoplasms / Q000532:radiotherapy / Q000628:therapy*; D011401:Promoter Regions, Genetic / Q000528:radiation effects*; D011859:Radiography; D018139:Simplexvirus / Q000201:enzymology; D013937:Thymidine Kinase / Q000235:genetics
chemical_list: D013937:Thymidine Kinase; D043525:Cytosine Deaminase