Synthetic blood group antigens for anti-A removal device and their interaction with monoclonal anti-A IgM.
Removal of blood group antibodies against the donor organ prior to ABO-incompatible transplantation can prevent episodes of hyperacute rejection. We are developing a specific antibody filter (SAF) device consisting of immobilized synthetic Atrisaccharide antigens conjugated to polyacrylamide (Atri-PAA) to selectively remove anti-A antibodies directly from whole blood. In this study, we evaluated eight anti-A IgM monoclonal antibodies (mAbs) using Enzyme-Linked Immunosorbent Assay (ELISA) to determine their specificity for binding to Atri-PAA. Five of the eight mAbs met our criteria for specificity by binding to Atri-PAA with at least five times greater affinity compared to the negative controls. These selected mAbs will be studied for their binding characteristics to Atri-PAA which will aid in the development of the SAF. The study of kinetics of antibody removal and quantification of antibody removal will be used in our mathematical model to maximize the antibody removal rate and binding capacity of the SAF.
Solovan
Jennifer C
JC
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15203, USA.
Oh
Heung-Il
HI
Alikhani
Azadeh
A
Gautam
Shalini
S
Vlasova
Katherine
K
Korchagina
Elena Y
EY
Bovin
Nicolai V
NV
Federspiel
William J
WJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D001789:Blood Group Antigens / Q000276:immunology*; D001787:Blood Group Incompatibility; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration / Q000295:instrumentation* / Q000379:methods; D006084:Graft Rejection / Q000517:prevention & control; D007070:Immunoglobulin A / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D007070:Immunoglobulin A; D007075:Immunoglobulin M