Quantification of organic eluates from polymerized resin-based dental restorative materials by use of GC/MS.
Residual monomers, additives and degradation products from resin-based dental restorative materials eluted into the oral cavity may influence the biocompatibility of these materials. Emphasis has been placed on studies addressing cytotoxic, genotoxic and estrogenic potential of these substances. A prerequisite for analyzing the potential of exposure to eluted compounds from dental materials is reliable quantification methods, both real time and accelerated measurements. The purpose of the present study was to quantify nine eluates; 2-hydroxyethyl methacrylate (HEMA), hydroquinone monomethyl ether (MEHQ), camphorquinone (CQ), butylated hydroxytoluene (BHT), ethyl 4-(dimethylamino)benzoate (DMABEE), triethylene glycoldimethacrylate (TEGDMA), trimethylolpropane trimethacrylate (TMPTMA), oxybenzone (HMBP) and drometrizole (TIN P) leaching from specimens of four commonly used resin-based dental materials in ethanol and an aqueous solution. All analyses were performed by use of GC/MS, each component was quantified separately and the results presented in microg mm(-2). This study has shown that elution from various materials differs significantly, not only in the types of eluates, but also regarding amounts of total and of single components. A high amount of HMBP, a UV stabilizer with potential estrogenic activity, was detected from one material in both solutions.
Michelsen
Vibeke Barman
VB
Department of Oral Sciences, Cariology, Faculty of Dentistry, University of Bergen, Armauer Hansens Hus, 5021 Bergen, Norway. vibeke.michelsen@odont.uib.no
Moe
Grete
G
Skålevik
Rita
R
Jensen
Einar
E
Lygre
Henning
H
D002138:Calibration; D003764:Dental Materials / Q000737:chemistry*; D003793:Dental Restoration, Permanent; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009930:Organic Chemicals / Q000032:analysis*; D011108:Polymers / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003764:Dental Materials; D009930:Organic Chemicals; D011108:Polymers