Fiber-based single cell analysis of reporter gene expression in yeast two-hybrid systems.
Yeast two-hybrid (Y2H) systems conventionally are used when screening for protein-protein interactions. A recurring problem with Y2H systems is the high prevalence of both false positives and false negatives. Distinguishing between real results and artifacts to characterize a protein-protein interaction properly can often be time-consuming and laborious. In this article, we report a new method for statistically evaluating single cell reporter gene transcriptional activation and expression in yeast two-hybrid systems. The system is based on statistical analysis of many yeast cells contained in a fiber-optic array, allowing for thorough characterization of reporter gene expression at different stringency levels.
Whitaker
Ragnhild Dragøy
RD
Department of Chemistry, Tufts University, Medford, MA 02155, USA.
Walt
David R
DR
D000704:Analysis of Variance; D005800:Genes, Fungal; D017930:Genes, Reporter; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D020798:Two-Hybrid System Techniques