title: Crassolides A-F, cembranoids with a trans-fused lactone from the soft coral Sarcophyton crassocaule.
abstract: Six new polyoxygenated cembrane-based diterpenoids possessing a trans-fused alpha-methylene-gamma-lactone, crassocolides A-F (1-6), have been isolated along with the known compound 7 from the ethyl acetate extract of a Taiwanese soft coral, Sarcophyton crassocaule. The structures of 1-6 have been established by detailed spectroscopic analysis, including 2D NMR (1H-1H COSY, HMQC, HMBC, and NOESY) spectroscopy, while their absolute configurations were determined using a modified Mosher's method for 1. The structure of 5 was further proven by X-ray diffraction analysis. The full assignment of NMR data of 7 is reported herein for the first time. The cytotoxicity of crassocolides 1-4, 6, and 7 against a limited panel of cancer cells was also determined.
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan, Republic of China.
authors.forename: Ho-Cheng
authors.initials: HC
authors.lastname: Huang
authors.forename: Atallah F
authors.initials: AF
authors.lastname: Ahmed
authors.forename: Jui-Hsin
authors.initials: JH
authors.lastname: Su
authors.forename: Chih-Hua
authors.initials: CH
authors.lastname: Chao
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
authors.forename: Michael Y
authors.initials: MY
authors.lastname: Chiang
authors.forename: Jyh-Horng
authors.initials: JH
authors.lastname: Sheu
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013237:Stereoisomerism
chemical_list: D000970:Antineoplastic Agents; D004224:Diterpenes