Genetic diversity analysis of traditional and improved Indonesian rice (Oryza sativa L.) germplasm using microsatellite markers.
The archipelago of Indonesia has a long history of rice production across a broad range of rice-growing environments resulting in a diverse array of local Indonesian rice varieties. Although some have been incorporated into modern breeding programs, the vast majority of these landraces remain untapped. To better understand this rich source of genetic diversity we have characterized 330 rice accessions, including 246 Indonesian landraces and 63 Indonesian improved cultivars, using 30 fluorescently-labeled microsatellite markers. The landraces were selected across 21 provinces and include representatives of the classical subpopulations of cere, bulu, and gundil rices. A total of 394 alleles were detected at the 30 simple sequence repeat loci, with an average number of 13 alleles per locus across all accessions, and an average polymorphism information content value of 0.66. Genetic diversity analysis characterized the Indonesian landraces as 68% indica and 32% tropical japonica, with an indica gene diversity of 0.53 and a tropical japonica gene diversity of 0.56, and a Fst of 0.38 between the two groups. All of the improved varieties sampled were indica, and had an average gene diversity of 0.46. A set of high quality Indonesian varieties, including Rojolele, formed a separate cluster within the tropical japonicas. This germplasm presents a valuable source of diversity for future breeding and association mapping efforts.
Thomson
Michael J
MJ
Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar 3A, Bogor, 16111, Indonesia.
Septiningsih
Endang M
EM
Suwardjo
Fatimah
F
Santoso
Tri J
TJ
Silitonga
Tiur S
TS
McCouch
Susan R
SR
D032461:Chromosomes, Plant / Q000235:genetics; D005456:Fluorescent Dyes; D005819:Genetic Markers; D014644:Genetic Variation; D007214:Indonesia; D018895:Microsatellite Repeats / Q000235:genetics*; D012275:Oryza / Q000145:classification / Q000166:cytology* / Q000235:genetics*; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D011157:Population Dynamics
D005456:Fluorescent Dyes; D005819:Genetic Markers