title: Genetic interaction of Gsc and Dkk1 in head morphogenesis of the mouse.
abstract: Mouse embryos lacking Gsc and Dkk1 function display severe deficiencies in craniofacial structures which are not found in either Dkk1 homozygous null or Gsc homozygous null mutant embryos. Loss of Gsc has a dosage-related effect on the severity of head truncation phenotype in Dkk1 heterozygous embryos. The synergistic effect of these mutations in enhancing head truncation provides direct evidence of a genetic interaction between Gsc and Dkk1, which display overlapping expression in the prechordal mesoderm. In the absence of Gsc activity, the expression of Dkk1, WNT genes and a transgenic reporter for WNT signalling are altered. Our results show that Gsc and Dkk1 functions are non-redundant in the anterior mesendoderm for normal anterior development and Gsc may influence Wnt signalling as a negative regulator.
authors.affiliation: Embryology Unit, Children’s Medical Research Institute, Locked Bag 23, Wentworthville, NSW 2145, Australia.
authors.forename: Samara L
authors.initials: SL
authors.lastname: Lewis
authors.forename: Poh-Lynn
authors.initials: PL
authors.lastname: Khoo
authors.forename: R
authors.initials: R
authors.lastname: Andrea De Young
authors.forename: Heidi
authors.initials: H
authors.lastname: Bildsoe
authors.forename: Maki
authors.initials: M
authors.lastname: Wakamiya
authors.forename: Richard R
authors.initials: RR
authors.lastname: Behringer
authors.forename: Mahua
authors.initials: M
authors.lastname: Mukhopadhyay
authors.forename: Heiner
authors.initials: H
authors.lastname: Westphal
authors.forename: Patrick P L
authors.initials: PP
authors.lastname: Tam