title: Bovine papillomavirus type-2 DNA and expression of E5 and E7 oncoproteins in vascular tumours of the urinary bladder in cattle.
abstract: Cattles suffering from chronic enzootic haematuria frequently develop urinary bladder tumours of both epithelial and mesenchymal origin mainly haemangioma and its malignant counterpart. The role of the bovine papillomavirus type-2 (BPV-2) and of its major transforming oncoprotein in naturally occurring urothelial carcinogenesis has been recently clarified. E5 interacts in vivo as in vitro with the beta receptor for the platelet-derived growth factor (PDGF). However, studies regarding tumours of mesenchymal origin such as those arising from blood vessels are lacking. We show that the BPV-2 is present in 100% of the vascular tumours of the urinary bladder examined. Twenty-six out of twenty-seven tumour samples (96%) expressed E5 while 20 out of 27 (74%) tumour samples expressed E7. The two viral oncoproteins were not expressed in normal endothelial cells. Additionally, they co-localize in neoplastic endothelial cells as demonstrated by confocal immunofluorescence. PDGFbeta receptor was also shown to be expressed and co-localizes with E5 in neoplastic blood vessels. Our results demonstrate, for the first time, that the BPV-2 is present in high percentage in tumours of mesenchymal origin arising in its natural host. Furthermore, the expression of the two viral oncoproteins confirm that the virus may have a causative role in the neoplastic process.
authors.affiliation: Department of Pathology and Animal health, University of Naples Federico II, Via F. Delpino, 1, 80137, Naples, Italy. borzacch@unina.it
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Borzacchiello
authors.forename: Valeria
authors.initials: V
authors.lastname: Russo
authors.forename: Claudia
authors.initials: C
authors.lastname: Spoleto
authors.forename: Sante
authors.initials: S
authors.lastname: Roperto
authors.forename: Liviu
authors.initials: L
authors.lastname: Balcos
authors.forename: Consuelo
authors.initials: C
authors.lastname: Rizzo
authors.forename: Aldo
authors.initials: A
authors.lastname: Venuti
authors.forename: Franco
authors.initials: F
authors.lastname: Roperto
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases; D004279:DNA, Viral / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000662:veterinary* / Q000821:virology*; D019043:Vascular Neoplasms / Q000235:genetics* / Q000821:virology*
chemical_list: D004279:DNA, Viral; D015513:Oncogene Proteins; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D020797:Receptor, Platelet-Derived Growth Factor beta