Differences in replication capacity between enterovirus 71 isolates obtained from patients with encephalitis and those obtained from patients with herpangina in Taiwan.
The cellular-tropism and biological characteristics of enterovirus 71 (EV71) isolates in Taiwan (TW) were studied. Growth curve experiments were conducted using cell lines that were possibly exhibited pathogenesis, and RT-PCR and sequencing tests were undertaken to amplify the 5' non-coding region (5'-NCR). The encephalitis isolate EV71 TW98NTU2078 was PBMC-tropic, temperature-resistant (Tr) at 40 degrees C, and easier to replicate in HTB-14 (astrocytoma) than the herpangina isolate EV71 TW98NTU1186 (The viral yields were 100-fold higher than those of the herpangina isolate EV71 TW98NTU1186 at 96 hr post infection.). The herpangina isolate EV71 TW98NTU1186 was non-PBMC-tropic, and temperature-sensitive (Ts) at 40 degrees C. The replication of EV71 TW98NTU1186 in HTB-14 was lower. No EV71 isolate infected HTB-37 (human colon adenocarcinoma cells). The encephalitis EV71 isolate exhibited better replication and transmission in PBMCs and astrocytes than did the EV71 isolate without CNS involvement.
Kung
Chien-Min
CM
Department of Medical Technology, Yuanpei University, Hsinchu, Taiwan, Republic of China.
King
Chwan-Chuen
CC
Lee
Chun-Nan
CN
Huang
Li-Min
LM
Lee
Ping-Ing
PI
Kao
Chuan-Liang
CL
D000818:Animals; D002522:Chlorocebus aethiops; D018792:Encephalitis, Viral / Q000150:complications* / Q000453:epidemiology; D004770:Enterovirus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000150:complications* / Q000453:epidemiology; D006557:Herpangina / Q000150:complications* / Q000453:epidemiology; D006801:Humans; D013624:Taiwan / Q000453:epidemiology; D014709:Vero Cells; D014779:Virus Replication