Partial molecular characterization of a mild isolate of Grapevine fanleaf virus from South Moravia, Czech Republic.
An atypical mild isolate HV5 of Grapevine fanleaf virus (GFLV) was found in a South Moravian viticulture region in Czech Republic. Partial sequence of its RNA2 was determined and compared with available sequences of typical GFLV isolates. Two genomic regions, namely a 814 nt-long one spanning the movement protein (MP) gene and a 5'-part of the coat protein (CP) gene. and a 1426 nt-long one covering a part of the CP gene and the adjacent 3'-non-coding region (3'-NCR) were analyzed. Although no HV5-specific molecular features could be found in the two regions, marked differences were observed in the 3'-NCR. There was a 54 nt-long portion in which the sequence identity of some compared isolates was only 54.7%. Moreover, an unique one-nucleotide deletion occurred in the HV5 3'-NCR. These changes were also reflected in the predicted RNA secondary structure of this region. Particular biological behavior of GFLV HV5 isolate, namely a symptomless infection, could be related to the observed molecular differences.
Komínek
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Research Institute of Crop Production, Drnovská 507, 161 06 Prague 6-Ruzyne, Czech Republic. kominek@vurv.cz
Bryxiová
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Glasa
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D020413:3' Untranslated Regions / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D018153:Czech Republic; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D053830:Plant Viral Movement Proteins / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D027843:Vitis / Q000821:virology*
D020413:3' Untranslated Regions; D036022:Capsid Proteins; D053830:Plant Viral Movement Proteins