Cloning, expression and identification of the gene of human single-chain variable fragment antibody against Hepatitis B virus surface antigen.
Expression of single-chain variable fragment (scFv) antibodies on the surface of bacteriophage is widely used to prepare antibodies with pre-defined specificities. A phage antibody library containing the gene for scFv antibody against Hepatitis B virus surface antigen (HBsAg) was panned with HBsAg immobilized on microtiter plate wells. After five rounds of panning 30 phage clones specific to HBsAg were obtained and one selected clone was sequenced. It was found to consist of 789 bp and its amino acid sequence and specifically detected the respective antigen in the patients but not in healthy persons.
Lun
Y Z
YZ
Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 110027, P.R. China.
Cheng
J
J
Zhong
Y W
YW
Zhao
B C
BC
D000595:Amino Acid Sequence; D000918:Antibody Specificity; D003001:Cloning, Molecular; D006510:Hepatitis B Antibodies / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007128:Immunoglobulin Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D019151:Peptide Library; D017422:Sequence Analysis, DNA
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D019151:Peptide Library