Chiral resolution of tryptophan derivatives by CE using canine serum albumin and bovine serum albumin as chiral selectors.
This work deals with the application of BSA and canine serum albumin (CSA) for enantioseparation of tryptophan derivatives with CE. The aim of this work was the investigation of the influence of different functional groups of tryptophan derivatives on enantioseparation. CSA as a chiral selector was tested to compare its selector properties with those of BSA. The enantiomers of the tryptophan derivatives were separated by adding BSA or CSA to the BGE. The influence of pH, temperature, BSA and CSA concentration and organic modifiers was investigated. It was found that the stereoselectivity for the different tryptophan derivatives is dependent on the albumin species. It turned out that the different functional groups of the derivatives showed a significant influence on stereoselectivity.
Hödl
Heike
H
Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Karl-Franzens University, Graz, Austria.
Koidl
Julia
J
Schmid
Martin G
MG
Gübitz
Gerald
G
D000818:Animals; D002417:Cattle; D004285:Dogs; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D012709:Serum Albumin / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013237:Stereoisomerism; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*
D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D014364:Tryptophan