LIF detection of peptides and proteins in CE.
CE- and microchip-based separations coupled with LIF are powerful tools for the separation, detection and determination of biomolecules. CE with certain configurations has the potential to detect a small number of molecules or even a single molecule, thanks to the high spatial coherence of the laser source which permits the excitation of very small sample volumes with high efficiency. This review article discusses the use of LIF detection for the analysis of peptides and proteins in CE. The most common laser sources, basic instrumentation, derivatization modes and set-ups are briefly presented and special attention is paid to the different fluorogenic agents used for pre-, on- and postcapillary derivatization of the functional groups of these compounds. A table summarizing major applications of these derivatization reactions to the analysis of peptides and proteins in CE-LIF and a bibliography with 184 references are provided which covers papers published to the end of 2005.
García-Campaña
Ana M
AM
Department of Analytical Chemistry, University of Granada, Granada, Spain. amgarcia@ugr.es
Taverna
Myriam
M
Fabre
Huguette
H
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D005470:Fluorometry; D007834:Lasers; D008164:Luminescent Proteins / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*
D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D010455:Peptides; D011506:Proteins