title: Effects of acute exhaustive physical exercise upon glutamine metabolism of lymphocytes from trained rats.
abstract: Transitory immunosupression is reported after intense exercise, especially after an increase in training overload and in overtraining. The influence of intense exercise on plasma hormones and glutamine concentration may contribute to this effect. However, the effect of such exercise-induced changes upon lymphocyte and glutamine metabolism is not known. We compared glutamine metabolism in lymphocytes in sedentary (SED) and trained rats. Rats from the moderate group (MOD) swam for 6 weeks, 1 h/day, in water at 32+/-1 degrees C, with a load of 5.5% body weight attached to the tail. Animals from the exhaustive group (EXT) trained like MOD, with training increasing to 3 times 1 h a day during the last week, with 150 min rest between each bout. Animals were killed immediately after the last training bout. We observed reduced concentrations of plasma glucose (p<0.05), glutamine (p<0.05), glutamate (p<0.05) in EXT compared to SED. In MOD, decreases in glutamine (p<0.05) were observed. Analyzing lymphocyte metabolism, we observed an increase in lactate production and glutamine consumption (p<0.05) in MOD (p<0.05) compared to SED and a decrease in glutamine consumption (p<0.05) and aspartate production in EXT. An increase in the proliferative response of lymphocytes in MOD and EXT was also observed when stimulated by ConA and LPS similarly to SED. Acute exercise promoted decreased glutamine plasma concentration and changes in glutamine metabolism that did not impair lymphocyte proliferation in exhaustive trained rats.
authors.affiliation: University of São Paulo, Institute of Biomedical Sciences, São Paulo, Brazil. rvca@usp.br
authors.forename: Ronaldo Vagner Thomatieli
authors.initials: RV
authors.lastname: Santos
authors.forename: Erico Chagas
authors.initials: EC
authors.lastname: Caperuto
authors.forename: Luis Fernando Bicudo Pereira
authors.initials: LF
authors.lastname: Costa Rosa
mesh_terms: D000641:Ammonia / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D049109:Cell Proliferation; D005080:Exercise Test; D018698:Glutamic Acid / Q000378:metabolism*; D005973:Glutamine / Q000097:blood; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D005082:Physical Exertion / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D005973:Glutamine; D018698:Glutamic Acid; D000641:Ammonia