title: Use of capillary electrophoresis and poly(ethylene oxide) as the coating agent for the determination of substances related to heroin addiction and treatment.
abstract: The purpose of this study was to identify and quantify morphine, codeine, methadone, and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine using capillary electrophoresis in urine specimens. Adequate peak separation was achieved using nearly neutral pH phosphate buffer and poly(ethylene oxide) as the coating agent. This dynamic coating of the inner surface of the capillary was obtained by rinsing with a solution containing this compound. The electroosmotic flow and the interactions between analytes and the capillary wall surface were reduced, while resolution and reproducibility were thus improved. Detection limits were appropriate for usual analytical requirements in forensic laboratories.
authors.affiliation: GeneDia S.r.l., Lammari, Lucca, Italy.
authors.forename: Niccoló
authors.initials: N
authors.lastname: Di Pietro
authors.forename: Giorgio
authors.initials: G
authors.lastname: Placanica
authors.forename: Italbo
authors.initials: I
authors.lastname: Fiorini
authors.forename: Clementina
authors.initials: C
authors.lastname: Manera
authors.forename: Caterina
authors.initials: C
authors.lastname: Orlando
authors.forename: Flaminia
authors.initials: F
authors.lastname: Palombo
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Saccomanni
authors.forename: Vittorio
authors.initials: V
authors.lastname: Salotti
authors.forename: Marco
authors.initials: M
authors.lastname: Macchia
mesh_terms: D003061:Codeine / Q000652:urine*; D019075:Electrophoresis, Capillary; D003932:Heroin / Q000378:metabolism; D006556:Heroin Dependence; D006801:Humans; D008691:Methadone / Q000652:urine*; D009020:Morphine / Q000652:urine*; D011092:Polyethylene Glycols; D011759:Pyrrolidines / Q000652:urine*; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D011759:Pyrrolidines; D011092:Polyethylene Glycols; D003932:Heroin; D009020:Morphine; D008691:Methadone; D003061:Codeine; C010724:2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine