Medium optimization by combination of response surface methodology and desirability function: an application in glutamine production.
An optimization strategy based on desirability function approach (DFA) together with response surface methodology (RSM) has been used to optimize production medium in L-glutamine fermentation. Fermentation problems often force to reach a compromise between different experimental variables in order to achieve the most suitable strategy applying in industrial production. The importance of the use of multi-objective optimization methods lies in the ability to cope with this kind of problems. A sequential RSM with different combinations of glucose and (NH(4))(2)SO(4) was performed to attain the optimal medium (OM-1) in glutamine production. Based on the result of RSM and the evaluation of production cost, a more economical optimal medium (OM-2) was obtained with the aid of DFA. In DFA study, glutamate, the main by-product in glutamine fermentation as another response was considered. Compared with OM-1 in validated experiment, similar amounts of glutamine were obtained in OM-2 while the concentration of glutamate and the production cost decreased by 53.6 and 7.1%, respectively.
Li
Jinshan
J
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
Ma
Cuiqing
C
Ma
Yanhe
Y
Li
Yan
Y
Zhou
Wei
W
Xu
Ping
P
D001709:Biotechnology / Q000191:economics / Q000379:methods; D048230:Corynebacterium glutamicum / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D018698:Glutamic Acid / Q000032:analysis; D005973:Glutamine / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism
D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D005973:Glutamine; D018698:Glutamic Acid; D005947:Glucose