Pneumococcal peptidoglycan-polysaccharides induce the expression of interleukin-8 in airway epithelial cells by way of nuclear factor-kappaB, nuclear factor interleukin-6, or activation protein-1 dependent mechanisms.
Cell envelope compounds of bacteria trigger immune and inflammatory reactions by way of chemokines/cytokines. In this study, we demonstrated that pneumococcal peptidoglycan-polysaccharides (PGPS) induced the production of interleukin (IL)-8 by way of nuclear factor (NF)-kappaB, nuclear factor interleukin (NF-IL)6, and activation protein (AP)-1 dependent mechanisms in the human bronchial epithelial cells (NL-20) in a dose- and time-dependent manner in vitro, and the mutation of either the NF-kappaB, NF-IL6, or AP-1 binding sites in the promoter of IL-8 abrogated the IL-8 transcriptional activity. In a similar way, lipopolysaccharides induced the promoter activation of IL-8 in NL-20. However, the PGPS-induced IL-8 promoter activation in rodent middle ear epithelial cells required NF-kappaB and NF-IL6 but not AP-1.
Tsuchiya
Katsuyuki
K
Department of Otolaryngology, University of Minnesota Otitis Media Research Center, University of Minnesota School of Medicine, Minneapolis, Minnesota, USA.
Toyama
Katsuhiro
K
Tsuprun
Vladimir
V
Hamajima
Yuki
Y
Kim
Youngki
Y
Ondrey
Frank G
FG
Lin
Jizhen
J
D000818:Animals; D001980:Bronchi / Q000166:cytology; D051743:CCAAT-Enhancer-Binding Protein-delta / Q000187:drug effects* / Q000378:metabolism; D002460:Cell Line; D004432:Ear, Middle / Q000166:cytology; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D012333:RNA, Messenger; D051381:Rats; D013296:Streptococcus pneumoniae; D018808:Transcription Factor AP-1 / Q000187:drug effects* / Q000378:metabolism
D016209:Interleukin-8; D008070:Lipopolysaccharides; D016328:NF-kappa B; D011135:Polysaccharides, Bacterial; D012333:RNA, Messenger; D018808:Transcription Factor AP-1; D051743:CCAAT-Enhancer-Binding Protein-delta