Dynamics of single-cell gene expression.
Cellular behavior has traditionally been investigated by utilizing bulk-scale methods that measure average values for a population of cells. Such population-wide studies mask the behavior of individual cells and are often insufficient for characterizing biological processes in which cellular heterogeneity plays a key role. A unifying theme of many recent studies has been a focus on the development and utilization of single-cell experimental techniques that are capable of probing key biological phenomena in individual living cells. Recently, novel information about gene expression dynamics has been obtained from single-cell experiments that draw upon the unique capabilities of fluorescent reporter proteins.
Longo
Diane
D
Department of Bioengineering, University of California at San Diego, La Jolla, CA 92093-0412, USA. hasty@ucsd.edu
Hasty
Jeff
J
D000818:Animals; D001412:Bacillus subtilis / Q000166:cytology*; D002477:Cells / Q000378:metabolism*; D004926:Escherichia coli / Q000166:cytology*; D005786:Gene Expression Regulation; D044085:Microfluidics