The physical and functional interaction of NDRG2 with MSP58 in cells.
NDRG2, a member of N-Myc downstream regulated gene family, exerts the important functions in cell differentiation and tumor suppression. Although the ectopic expressed Ndrg2 inhibits the proliferation of tumor cells, its intracellular signal transduction pathway is hardly known. Here, we identified MSP58, a 58-kDa microspherule protein, as an interacting partner of human Ndrg2 by using yeast two-hybrid screening. The interaction was confirmed by glutathione S-transferase pull-down assay in vitro and by co-immune-precipitation assay in vivo. The forkhead associated domain of MSP58 is essential for its interaction with Ndrg2. Ndrg2 could co-localize with MSP58 in nuclear of HeLa cell during cell stress. Furthermore, the modulation of Ndrg2 level influences the cell cycle process together with MSP58. In conclusion, the findings offered a novel insight into the physiological roles of Ndrg2.
Zhang
Jing
J
The Department of Biochemistry and Molecular Biology and The State Key Laboratory of Cancer Biology, The Fourth Military Medical University, Xi'an, China 710032, China.
Liu
Junye
J
Li
Xia
X
Li
Fuyang
F
Wang
Lifeng
L
Zhang
Jian
J
Liu
Xinping
X
Shen
Lan
L
Liu
Na
N
Deng
Yanchun
Y
Yang
Angang
A
Han
Hua
H
Zhao
Mujun
M
Yao
Libo
L
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D002522:Chlorocebus aethiops; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins
C113292:MCRS1 protein, human; C466228:NDRG2 protein, human; D009687:Nuclear Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; D025521:Tumor Suppressor Proteins