title: [The influence of AMPs from HaCaT keratinocytes on some bacteria strains derived from skin changes].
abstract: The aim of this study was to evaluate antimicrobial activity of protein extracts from HaCaT cell line against bacterial strains, isolated from clinical materials, obtained from patients with clinical symptoms of acne (Propionibacterium acnes) and gas gangrene (Clostridium perfringens and Sterptococcus pyogenes). Reference strain of Staphylococcus aureus ATCC 25923 also was used. Protein extracts from cultured HaCaT cells were obtained by 3-fold freezing/defreezing cells in dry ice following by centrifugation and incubated with appropriate bacterial suspension (0.5 McFarland scale) during 6 and 24 hours. We observed time-depending and strain-depending activity of HaCaT--protein extract. Interestingly, high activity was demonstrated against strains of S. pyogenes and C. perfringens. Because of increasing bacterial resistance to antibiotics further studies in the field of antimicrobial peptides are required.
authors.affiliation: Katedra i Zakład Mikrobiologii Lekarskiej Slaskiej Akademii Medycznej w Katowicach.
authors.forename: Barbara
authors.initials: B
authors.lastname: Wiechuła
authors.forename: Iwona
authors.initials: I
authors.lastname: Wilk
authors.forename: Alicja
authors.initials: A
authors.lastname: Ekiel
authors.forename: Jerzy
authors.initials: J
authors.lastname: Tustanowski
authors.forename: Gayane
authors.initials: G
authors.lastname: Martirosian
mesh_terms: D000152:Acne Vulgaris / Q000382:microbiology; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D023181:Antimicrobial Cationic Peptides / Q000276:immunology* / Q000494:pharmacology*; D003872:Dermatitis / Q000382:microbiology; D005738:Gas Gangrene / Q000382:microbiology; D006801:Humans; D015603:Keratinocytes / Q000276:immunology*; D008826:Microbial Sensitivity Tests; D011425:Propionibacterium acnes / Q000502:physiology; D012867:Skin / Q000382:microbiology; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000276:immunology*; D013210:Staphylococcus / Q000187:drug effects* / Q000276:immunology; D013211:Staphylococcus aureus / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides