title: Chaetominine, a cytotoxic alkaloid produced by endophytic Chaetomium sp. IFB-E015.
abstract: Chaetominine (1), an alkaloidal metabolite with a new framework, was characterized from the solid-substrate culture of Chaetomium sp. IFB-E015, an endophytic fungus on the apparently healthy Adenophora axilliflora leaves. Its structure was determined by a combination of its spectral data and single-crystal X-ray diffraction analysis, with its absolute configuration elucidated by Marfey's method. Chaetominine was more cytotoxic than 5-fluorouracil against the human leukemia K562 and colon cancer SW1116 cell lines. [structure: see text]
authors.affiliation: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, People's Republic of China.
authors.forename: Rui H
authors.initials: RH
authors.lastname: Jiao
authors.forename: Shu
authors.initials: S
authors.lastname: Xu
authors.forename: Jun Y
authors.initials: JY
authors.lastname: Liu
authors.forename: Hui M
authors.initials: HM
authors.lastname: Ge
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.forename: Chen
authors.initials: C
authors.lastname: Xu
authors.forename: Hai L
authors.initials: HL
authors.lastname: Zhu
authors.forename: Ren X
authors.initials: RX
authors.lastname: Tan
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification / Q000494:pharmacology*; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D029422:Campanulaceae / Q000382:microbiology; D045744:Cell Line, Tumor; D002597:Chaetomium / Q000737:chemistry*; D002942:Circular Dichroism; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology; D018360:Crystallography, X-Ray; D004354:Drug Screening Assays, Antitumor; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D026121:Indole Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D020014:K562 Cells; D009682:Magnetic Resonance Spectroscopy; D018515:Plant Leaves / Q000382:microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000903:Antibiotics, Antineoplastic; D000964:Antimetabolites, Antineoplastic; D026121:Indole Alkaloids; D013778:Tetrazolium Salts; D013844:Thiazoles; C522312:chaetominine; C022616:thiazolyl blue; D005472:Fluorouracil