[A new real-time method monitoring behaviors of living cells in vitro-PCC].
A method combinating piezoelectric-biochip technique with cell culture technique in vitro has been developed. Piezoelectric cell-based chip (PCC) can continously monitor the behaviors of living cells attached to or spreaded on surfaces. The method measures the resonant frequency (f), the dissipation energy (D) and impedance (Z), in real time. These multi parameters can inflect information of cells qualitively and quantitively. This paper covers principle, materials and methods, results and discussion of the new technique, simultaniously indicates its application and trends.
Wei
Xiaolan
X
College of Bimedical Engineering of Chongqing University, Chongqing 400044, China.
Mo
Zhihong
Z
D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods; D017097:Electric Impedance; D004566:Electrodes; D066298:In Vitro Techniques; D013997:Time Factors