A microfluidic-based system for analysis of single cells based on Ca2+ flux.
A microfluidic format-based system has been developed for in situ monitoring of the calcium flux response to agonists using Chinese hamster ovary (CHO) cells. The assay is based on measuring the fluorescent intensity of the calcium-sensitive indicator, Fluo-4 AM, and was performed in a modified glass chip channel, whose surface was functionalised using a silanisation method with 3-aminopropyltriethoxysilane (APTS) (enabling the cells to be immobilised on the channel surface). CHO cells calcium flux response was measured for different agonists over a range of concentrations. Cells and reagents were introduced into the chip in a continuous flow as a series of plugs in a given sequence.
Zhang
Xunli
X
Department of Chemistry, The University of Hull, Hull, UK.
Yin
Huabing
H
Cooper
Jon M
JM
Haswell
Stephen J
SJ
D000814:Aniline Compounds / Q000032:analysis; D000818:Animals; D001681:Biological Assay; D016466:CHO Cells; D002118:Calcium / Q000032:analysis*; D002463:Cell Membrane Permeability / Q000187:drug effects; D018914:Cells, Immobilized / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D005456:Fluorescent Dyes / Q000032:analysis; D015759:Ionomycin / Q000494:pharmacology; D044085:Microfluidics / Q000295:instrumentation*; D014544:Uridine Triphosphate / Q000494:pharmacology; D014966:Xanthenes / Q000032:analysis
D000814:Aniline Compounds; C409648:Fluo 4; D005456:Fluorescent Dyes; D014966:Xanthenes; D015759:Ionomycin; D002118:Calcium; D014544:Uridine Triphosphate