Ca channel currents inhibited by serum from select patients with Guillain-Barré syndrome.
We performed an electrophysiological study demonstrating inhibition of spontaneous muscle action potentials within a coculture of rat muscle and spinal cord by exposure to serum, as well as purified IgG, from patients with the acute motor axonal neuropathy (AMAN) variant of Guillain-Barré syndrome (GBS). However, exposure to serum from two patients with the acute inflammatory demyelinating polyneuropathy (AIDP) form of GBS had no effect. Using a whole-cell recording technique, we then investigated the effects of serum and purified IgG from patients with GBS on voltage-dependent calcium channel (VDCC) currents in nerve growth factor-differentiated PC12 cells. Serum from patients with GBS (AMAN) inhibited VDCC currents in PC12 cells, which was fully reversible by washing with the bath solution. Similarly, purified IgG from the serum of two patients with GBS (AMAN) also inhibited VDCC currents in PC12 cells. In contrast, sera from patients with AIDP and healthy volunteers did not affect VDCC currents in PC12 cells. These results suggest that muscle weakness in some patients with GBS might be induced by inhibition of Ca2+ channel currents within motor nerve terminals.
Nakatani
Y
Y
Department of Pharmacotherapeutics, Showa Pharmaceutical University, Machida, Tokyo, Japan.
Kawakami
K
K
Nagaoka
T
T
Utsunomiya
I
I
Tanaka
K
K
Yoshino
H
H
Miyatake
T
T
Hoshi
K
K
Taguchi
K
K
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D018920:Coculture Techniques; D020275:Guillain-Barre Syndrome / Q000097:blood* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D032342:Muscle Cells / Q000276:immunology / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000276:immunology / Q000378:metabolism; D009469:Neuromuscular Junction / Q000276:immunology / Q000378:metabolism*; D009474:Neurons / Q000276:immunology / Q000378:metabolism*; D016716:PC12 Cells; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D044967:Serum; D013116:Spinal Cord / Q000276:immunology / Q000378:metabolism
D015220:Calcium Channels; D007074:Immunoglobulin G