title: A proteomic analysis of the effect of MAPK pathway activation on L-glutamate-induced neuronal cell death.
abstract: Oxidative stress has been implicated in the pathogenesis of neuronal degenerative diseases. It is also widely known that oxidative stress induces mitogen-activated protein kinase (MAPK) signaling cascades. In this study, we used proteomic analysis to investigate the role of the MAPK pathway in oxidative stress-induced neuronal cell death. The results demonstrated that several proteins, including eukaryotic translation elongation factor 2 (eEF2) and enolase I, showed a differential expression pattern during the neuronal cell death process, and this was MAPK pathway dependent. Several chaperone and cytoskeletal proteins including heat shock protein 70, calreticulin, vimentin, prolyl 4-hydroxylase beta polypeptide, and transgelin 2 were up-or down-regulated, despite their expressions not depending on the MAPK pathway. These findings strongly suggest that the expressions of proteins which play protective roles are independent of the MAPK pathway. On the other hand, eEF2 and enolase I may be the downstream targets of the MAPK pathway.
authors.affiliation: Korean Research Institute of Bioscience and Biotechnology, 52 Eoeun-Dong, Yusung-Gu, Daejeon, Republic of Korea.
authors.forename: Sunghyun
authors.initials: S
authors.lastname: Kang
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Young Jae
authors.initials: YJ
authors.lastname: Bahn
authors.forename: Jin Woong
authors.initials: JW
authors.lastname: Chung
authors.forename: Do Hee
authors.initials: DH
authors.lastname: Lee
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Park
authors.forename: Tae-Sung
authors.initials: TS
authors.lastname: Yoon
authors.forename: Byoung Chul
authors.initials: BC
authors.lastname: Park
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
mesh_terms: D002070:Butadienes / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D004789:Enzyme Activation / Q000187:drug effects; D020869:Gene Expression Profiling; D018698:Glutamic Acid / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects*; D009570:Nitriles / Q000494:pharmacology; D040901:Proteomics
chemical_list: D002070:Butadienes; D009570:Nitriles; C113580:U 0126; D018698:Glutamic Acid; D020928:Mitogen-Activated Protein Kinases