Mitotic kinesin-like protein 2 binds and colocalizes with papillomavirus E2 during mitosis.
MKlp2 is a kinesin-like motor protein of the central mitotic spindle required for completion of cytokinesis. Papillomavirus E2 is a sequence specific DNA binding protein that regulates viral transcription and replication and is responsible for partitioning viral episomes into daughter cells during cell division. We demonstrate that MKlp2 specifically associates with the E2 protein during mitosis. Using chromatin immunoprecipitation, we show viral genomes are in complex with MKlp2 only within this stage of cell cycle. By immunofluorescence, a subpopulation of papillomavirus E2 colocalizes with MKlp2 in the midbody/midplate during late mitosis. We conclude that during specific stages of mitosis, the papillomavirus E2 protein binds to MKlp2, and infer that association with this motor protein ensures viral genome partitioning during cytokinesis.
Yu
Ting
T
Department of Medicine, University of Massachusetts Medical School, 364 Plantation St., Worcester, MA 01605, USA.
Peng
Yu-Cai
YC
Androphy
Elliot J
EJ
D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D008938:Mitosis; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D027383:Papillomaviridae / Q000502:physiology*; D030361:Papillomavirus Infections / Q000821:virology; D011485:Protein Binding; D021381:Protein Transport; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
D004268:DNA-Binding Proteins; C051550:E2 protein, Bovine papillomavirus; C486567:KIF15 protein, human; D009856:Oncogene Proteins, Viral; D014764:Viral Proteins; C076036:oncogene protein E2, Human papillomavirus type 1; D016547:Kinesins