title: Function of two beta-carotenes near the D1 and D2 proteins in photosystem II dimers.
abstract: The antenna proteins in photosystem II (PSII) not only promote energy transfer to the photosynthetic reaction center (RC) but provide also an efficient cation sink to re-reduce chlorophyll a if the electron transfer (ET) from the Mn-cluster is inhibited. Using the newest PSII dimer crystal structure (3.0 A resolution), in which 11 beta-carotene molecules (Car) and 14 lipids are visible in the PSII monomer, we calculated the redox potentials (Em) of one-electron oxidation for all Car (Em(Car)) by solving the Poisson-Boltzmann equation. In each PSII monomer, the D1 protein harbors a previously unlocated Car (CarD1) in van der Waals contact with the chlorin ring of ChlZ(D1). Each CarD1 in the PSII dimer complex is located in the interface between the D1 and CP47 subunits, together with another four Car of the other PSII monomer and several lipid molecules. The proximity of Car bridging between CarD1 and plastoquinone/Q(A) may imply a direct charge recombination of Car+Q(A)-. The calculated Em(CarD1) and Em(ChlZ(D1)) are, respectively, 83 and 126 mV higher than Em(CarD2) and Em(ChlZ(D2)), which could explain why CarD2+ and ChlZ(D2)+ are observed rather than the corresponding CarD1+ and ChlZ(D1)+.
authors.affiliation: Institute of Chemistry and Biochemistry/Crystallography, Free University of Berlin, Berlin, Germany. hzi1@psu.edu <hzi1@psu.edu>
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikita
authors.forename: Bernhard
authors.initials: B
authors.lastname: Loll
authors.forename: Jacek
authors.initials: J
authors.lastname: Biesiadka
authors.forename: Jan
authors.initials: J
authors.lastname: Kern
authors.forename: Klaus-Dieter
authors.initials: KD
authors.lastname: Irrgang
authors.forename: Athina
authors.initials: A
authors.lastname: Zouni
authors.forename: Wolfram
authors.initials: W
authors.lastname: Saenger
authors.forename: Ernst-Walter
authors.initials: EW
authors.lastname: Knapp
mesh_terms: D000458:Cyanobacteria / Q000737:chemistry*; D004579:Electron Transport / Q000502:physiology; D008956:Models, Chemical; D008958:Models, Molecular; D010084:Oxidation-Reduction; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000378:metabolism; D019207:beta Carotene / Q000737:chemistry* / Q000378:metabolism
chemical_list: D045332:Photosystem II Protein Complex; D019207:beta Carotene