title: Immunoglobulin class (IgG, IgM) determination by dithiothreitol in sensitized kidney transplant candidates.
abstract: Immunoglobulin class plays an important role in the histocompatibility crossmatch test to predict hyperacute rejection in kidney transplantation. The existing data indicates that immunoglobulin (Ig) M antibodies, particularly when they are autoantibodies, are not deleterious to the renal allograft. We used the reducing agent dithiotreitol (DTT) to inactivate IgM but not IgG in the crossmatch assay to help sensitized patients have the chance for successful transplantation. In this descriptive study, 57 candidates for kidney transplantation with final positive crossmatches who had a history of panel-reactive antibody (PRA) greater than 30% were selected. Two of 57 patients had systemic lupus erythematous (SLE). The sera of patients were treated by DTT and then measured for cytotoxicity against donor lymphocytes and a panel of 12 cells using the complement-dependent cytotoxicity (CDC) method. Autocrossmatch was also performed to differentiate autoantibodies and alloantibodies by the CDC method. Of the 57 patients, six subjects (10.53%) had IgM and 51 patients (89.47%) IgG in their serum against donor lymphocytes. Also against panel cells, 39 of 57 patients (68.43%) had IgG, three patients (5.26%) had IgM, and 15 patients (26.31%) had both IgG and IgM antibodies. Autolymphocytotoxic antibodies were detected in 1.75% patients (1 of 57) who had SLE. According to our results, 5.26% of the patients who were IgM-positive and IgG-negative for both crossmatch and PRA assays may experience successful kidney transplantation.
authors.affiliation: Department of Immunology, Medicine Faculty, Isfahan University of Medical Sciences, Isfahan, Iran. khooladadi@royaninstitute.org
authors.forename: L
authors.initials: L
authors.lastname: Khodadadi
authors.forename: M
authors.initials: M
authors.lastname: Adib
authors.forename: A
authors.initials: A
authors.lastname: Pourazar
mesh_terms: D003602:Cytotoxicity, Immunologic; D004229:Dithiothreitol; D006650:Histocompatibility Testing; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007132:Immunoglobulin Isotypes / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood*; D007202:Indicators and Reagents; D016030:Kidney Transplantation / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000276:immunology; D014850:Waiting Lists
chemical_list: D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D007202:Indicators and Reagents; D004229:Dithiothreitol