Genotyping of Edwardsiella tarda isolated from freshwater fish culture system.
The applicability of PCR-RFLP of 16S rDNA and conventional phenotypic methods for differentiation of Edwardsiella tarda associated in freshwater fish culture system was studied. In this study, by conventional biochemical tests and antibiotic resistant patterns 2 and 14 groups were obtained. But these methods failed to discriminate the isolates habitat wise. However, PCR-RFLP of 16S rDNA was found to be specific to detect habitat-specific isolates. All the fish isolates belonging to particular genotypes were found only in fish, not in water or sediment. Some of the genotypes were exclusively present in water and sediment. This study indicates the prevalence of site-specific genotypes in freshwater ecosystems. Molecular method is found to be superior to discriminate the E. tarda habitat wise to conventional typing methods.
Acharya
Madushmita
M
Microbiology Section, Central Institute of Freshwater Aquaculture, Kaushalyaganga Bhubaneswar 751002, Orissa, India.
Maiti
N K
NK
Mohanty
S
S
Mishra
Pushpita
P
Samanta
M
M
D000818:Animals; D017756:Aquaculture; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D020609:Edwardsiella tarda / Q000145:classification / Q000235:genetics*; D005399:Fishes / Q000382:microbiology*; D005838:Genotype; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal