Biotransformation enzymes in Cunninghamella blakesleeana (NCIM-687).
Presence of higher enzyme levels of aminopyrine N-demethylase, aniline hydroxylase and 11-beta hydroxylase activities were observed in Cunninghamella blakesleeana grown in potato-dextrose medium for 96 h. The enzyme activity preferred NADPH as a cofactor and showed inhibition with CO, indicating cytochrome P450 mediated reactions. A significant increase in aniline hydroxylase enzyme activity was observed when mycelia incubated in incubation medium containing different inducers (viz. camphor, cholesterol, naphthalene, veratrole, phenobarbital, n -hexadecane and ethyl alcohol) when compared with mycelia incubated in same way but in absence of inducers. Cunninghamella blakesleeana (NCIM 687) have shown the ability to degrade cholesterol, camphor and naphthalene when 96 h grown mycelia incubated in incubation medium containing these organic compounds.
Bhosale
Sanjyot
S
Department of Biochemistry, Shivaji University, Kolhapur-416 004, India.
Saratale
Ganesh
G
Govindwar
Sanjay
S
D000633:Aminopyrine N-Demethylase / Q000378:metabolism*; D000815:Aniline Hydroxylase / Q000378:metabolism*; D001711:Biotransformation; D002164:Camphor / Q000493:pharmacokinetics; D002784:Cholesterol / Q000493:pharmacokinetics; D020093:Cunninghamella / Q000201:enzymology* / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D015786:Cytochromes b5 / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009281:Naphthalenes / Q000493:pharmacokinetics
D009281:Naphthalenes; C031721:naphthalene; D002164:Camphor; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D006899:Mixed Function Oxygenases; D000815:Aniline Hydroxylase; D000633:Aminopyrine N-Demethylase