title: Quantitative determination of gland mucous cells-type mucin using a monoclonal antibody, HIK1083: its pathophysiological changes in human gastric juice.
abstract: BACKGROUND
  Pathological alteration in gastric mucosa is caused by Helicobacter pylori infection and is detectable by histological analysis. In particular, the alteration of gland mucous cells (GMCs)-type mucin, which plays a protective role against H. pylori infection, is critical in the pathogenesis of H. pylori-related gastritis. We established an assay for GMCs-type mucin and quantitatively assessed the pathophysiological changes in its content in human gastric juice samples.
  
  
  METHODS
  The assay method for GMCs-type mucin was based on ELISA using a monoclonal antibody (HIK1083), and was used it to measure GMCs-type mucin in gastric juice obtained from patients with or without H. pylori infection.
  
  
  RESULTS
  All the basic characteristics of the current method were satisfactory to quantify the GMCs-type mucin content in gastric juice. The GMCs-type mucin content, but not total mucin content, was significantly higher in patients with H. pylori infection (n=17; 437+/-476 U, mean+/-SD) than in those without H. pylori infection (n=55; 168+/-322 U, p<0.05).
  
  
  CONCLUSIONS
  The current method is suitable for the quantitative analysis of GMCs-type mucin in gastric juice. The change in GMCs-type mucin content in gastric juice may be possibly implicated in the pathophysiology of the gastric mucosa and in the patient's gastric mucosal lesions.
authors.affiliation: Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
authors.forename: Seiko
authors.initials: S
authors.lastname: Kubota
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Toshiko
authors.initials: T
authors.lastname: Kumagai
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Sugano
authors.forename: Kenji
authors.initials: K
authors.lastname: Kawasaki
authors.forename: Minoru
authors.initials: M
authors.lastname: Tozuka
authors.forename: Taiji
authors.initials: T
authors.lastname: Akamatsu
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsuzawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sugiyama
authors.forename: Makoto
authors.initials: M
authors.lastname: Kurihara
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Katsuyama
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ota
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay; D005750:Gastric Juice / Q000276:immunology / Q000378:metabolism*; D005752:Gastric Mucins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D005753:Gastric Mucosa / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013272:Stomach Diseases / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology*; D013552:Swine; D013696:Temperature
chemical_list: D000911:Antibodies, Monoclonal; D005752:Gastric Mucins