Repeated use of immobilized Gluconobacter oxydans cells for conversion of glycerol to dihydroxyacetone.
Dihydroxyacetone (DHA) is of great interest in the fine chemical and pharmaceutical industry; therefore, the discovery of suitable biocatalysts for the efficient production of it is very necessary. In the experiment, Gluconobacter oxydans was immobilized in polyvinyl alcohol (PVA). Various parameters of the immobilized cells were investigated. The results have shown that the optimal conversion conditions by the immobilized cells were at 30 degrees C and pH 6.0. The immobilized cells remained very active over the period of 14 days for storage and only lost 10% of its original activity. Repeated use of immobilized cells for conversion of glycerol to DHA was carried out in a 1.5 L stirred tank reactor, the average conversion rate was about 86%. Despite the high shear stress, bead shape was not affected, even after five consecutive conversion cycles. The regenerated biocatalyst could recover 90% of its initial activity.
Wei
Shenghua
S
State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, PR China.
Song
Qingxun
Q
Wei
Dongzhi
D
D019149:Bioreactors / Q000382:microbiology; D002384:Catalysis; D018914:Cells, Immobilized; D004098:Dihydroxyacetone / Q000096:biosynthesis* / Q000737:chemistry; D020600:Gluconobacter oxydans / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D005990:Glycerol / Q000737:chemistry / Q000378:metabolism*; D007218:Industrial Microbiology; D011142:Polyvinyl Alcohol / Q000737:chemistry
D011142:Polyvinyl Alcohol; D004098:Dihydroxyacetone; D005990:Glycerol