title: DNA vaccine-encoded glycoprotein B of HSV-1 fails to protect chronic morphine-treated mice against HSV-1 challenge.
abstract: The use of morphine has been demonstrated to increase susceptibility to infections. Herpes simplex virus type 1 (HSV-1) is a highly successful pathogen among immunocompromised individuals. In the present study, due to the importance of HSV vaccination in morphine abusers, the effects of chronic morphine exposure on the host response to a HSV-1 gB DNA-based vaccine have been investigated. The study is addressing an important aspect of vaccine development among the susceptible (immunocompromised) hosts. BALB/c mice were exposed to morphine over 11 days. They were then vaccinated with DNA vaccine or KOS strain as a live vaccine. The findings showed that the morphine-treated animals failed to respond to DNA vaccination evaluated by the anti-HSV gB antibody titer, delayed type hypersensitivity (DTH) and lethal HSV-1 challenge. Under the same conditions, the KOS vaccine showed a reduced Ab titer and DTH response in morphine-treated mice, but could protect mice against the lethal challenge and was safe for vaccination of morphine-treated animals.
authors.affiliation: Department of Virology, School of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-111, Tehran, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Jamali
authors.forename: Mohammad H
authors.initials: MH
authors.lastname: Roostaee
authors.forename: Hoorieh
authors.initials: H
authors.lastname: Soleimanjahi
authors.forename: Firouz
authors.initials: F
authors.lastname: Ghaderi Pakdel
authors.forename: Taravat
authors.initials: T
authors.lastname: Bamdad
mesh_terms: D000701:Analgesics, Opioid / Q000009:adverse effects; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002522:Chlorocebus aethiops; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D006561:Herpes Simplex / Q000276:immunology / Q000517:prevention & control*; D022301:Herpes Simplex Virus Vaccines / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D016867:Immunocompromised Host; D051379:Mice; D008807:Mice, Inbred BALB C; D009020:Morphine / Q000009:adverse effects; D011897:Random Allocation; D019444:Vaccines, DNA / Q000276:immunology*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000701:Analgesics, Opioid; D000914:Antibodies, Viral; D006023:Glycoproteins; D022301:Herpes Simplex Virus Vaccines; D019444:Vaccines, DNA; D014764:Viral Proteins; D009020:Morphine