Phytochemical and biological investigation of Aristolochia maurorum L.
Aristolochia maurorum L. of Jordanian origin has been investigated phytochemically, quantitatively, and biologically. Three atypical alkaloids, namely aristolochic acid I (1), aristolochic acid II (2) and aristolochic acid IIIa (3), have been isolated and identified. Of these known 1-phenanthrenecarboxylic acids, 2 and 3 are reported for the first time from this species. The identified compounds 1-3 were first evaluated biologically as cytotoxic agents against the brine shrimp lethality test (BST), in which compound 1 was found to be the most potent (LC50, 4.9 microg/mL). The antiplatelet activity of the methanolic extracts, the acidic fractions of aerial and root parts, and the identified compounds 1-3 were evaluated using an automatic platelet aggregometer and coagulation tracer (APACT 2). Using external reference standards, and a reverse-phase isocratic method, the distribution of aristolochic acid I and aristolochic acid II in different plant parts of Aristolochia maurorum L. during flowering stage was analyzed by PDA-HPLC. A quantitative comparison between two previously reported extraction methods was also made. Roots were found to be the main storage of aristolochic acid I and aristolochic acid II during flowering stage with about 0.22 and 0.108% (w/w), respectively.
Alali
Feras Q
FQ
Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, PO. Box 3030, Irbid 22110, Jordan. Falali@just.edu.jo
Tawaha
Khaled
K
Shehadeh
Mayadah B
MB
Telfah
Suha
S
D002212:Capsicum / Q000187:drug effects / Q000821:virology*; D002244:Carbon / Q000378:metabolism; D002734:Chlorophyll / Q000032:analysis; D006540:Herbicides / Q000494:pharmacology*; D010788:Photosynthesis / Q000187:drug effects; D010942:Plant Viruses / Q000187:drug effects*; D010944:Plants / Q000378:metabolism* / Q000821:virology*
D006540:Herbicides; D002734:Chlorophyll; D002244:Carbon