A semiautomatic instrument for the radioautographic coating technique.
By means of a mechanical coating instrument a fast, simple method to coat specimens with liquid nuclear track emulsion has been devised for quantitative light and electron microscopic radioautography. In both cases, the section is mounted on a glass slide. After the vertically held slide has been immersed in the melted emulsion, the instrument withdraws it at a slow, constant speed. As a result, the specimen is coated with a thin, uniform emulsion layer composed of homogeneously distributed silver bromide crystals. The thickness of the emulsion coat may be standardized by selection of an optimal combination of emulsion dilution, temperature and withdrawal speed.
Kopriwa
B M
BM
Department of Anatomy, McGill University, Montreal, Canada.
D001345:Autoradiography / Q000295:instrumentation / Q000379:methods; D001965:Bromides / Q000737:chemistry; D004655:Emulsions / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D008099:Liver / Q000737:chemistry / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity; D018030:Silver Compounds / Q000737:chemistry; D013048:Specimen Handling / Q000379:methods; D013499:Surface Properties
D001965:Bromides; D004655:Emulsions; D018030:Silver Compounds; D005780:Gelatin; C047751:silver bromide