title: The association of single nucleotide polymorphisms in the MMP-9 genes with susceptibility to acute primary angle closure glaucoma in Taiwanese patients.
abstract: OBJECTIVE
  To study the relationships between single nucleotide polymorphisms (SNPs) of extracellular matrix, matrix metalloproteases (MMPs), tissue inhibitors of MMPs, and other glaucoma-associated genes and acute primary angle closure glaucoma (PACG).
  
  
  METHODS
  We extracted DNA samples from 78 adult patients with acute PACG and 86 control subjects to study the relationships between these specific genes and acute PACG. Genotyping was performed for 35 genes by the GenomeLab SNPstream genotyping system after PCR amplification of chromosomal DNA. The association between these genetic polymorphisms and risk of primary PACG was estimated by chi2 and logistic regression.
  
  
  RESULTS
  The genotyping success rate was 99%. Genotyping for the MMP9 site (rs2664538) was significantly different between the two groups (p=0.000001) and the odds ratio was 2.586 (95% CI: 1.715-3.898, p<0.00001). However, there were no associations of SNPs to other genes in patients with acute PACG.
  
  
  CONCLUSIONS
  Our results reveal that SNP rs2664538, which is located at the MMP9 gene, is likely to be associated with acute PACG.
authors.affiliation: Department of Ophthalmology, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: I-Jong
authors.initials: IJ
authors.lastname: Wang
authors.forename: Ting-Hsuan
authors.initials: TH
authors.lastname: Chiang
authors.forename: Yung-Feng
authors.initials: YF
authors.lastname: Shih
authors.forename: Shao-Chun
authors.initials: SC
authors.lastname: Lu
authors.forename: Luke Long-Kuang
authors.initials: LL
authors.lastname: Lin
authors.forename: Jui-Wen
authors.initials: JW
authors.lastname: Shieh
authors.forename: Tsing-Hong
authors.initials: TH
authors.lastname: Wang
authors.forename: John R
authors.initials: JR
authors.lastname: Samples
authors.forename: Por-Tying
authors.initials: PT
authors.lastname: Hung
mesh_terms: D000208:Acute Disease; D000368:Aged; D044466:Asian People / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015812:Glaucoma, Angle-Closure / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D016015:Logistic Models; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D013624:Taiwan
chemical_list: D020780:Matrix Metalloproteinase 9