[Radiation-inducible promoters-mediated cdglytk gene in the treatment of buccal carcinoma in golden hamster].
OBJECTIVE
To observe the therapeutic effect of CDglyTK gene mediated by radiation-inducible promoters in the treatment of buccal carcinoma in Golden Hamster.


METHODS
Animal models of buccal carcinoma in golden hamster were established by painting 0.5% dimethyl-benzanthracene. The plasmids pcDNA (+) 3.1/E-CDglyTK were transfected into tumors by lipofectamine. 24 h later, the tumors were exposed to 3 Gy irradiation. Animals were monitored at regular intervals for volume of tumors. CDglyTK mRNA was assayed by RT-PCR. Apoptosis and proliferating cell nuclear antigen were detected respectively by in situ end-labeling and immunohistochemical methods.


RESULTS
Compared with control groups, the tumor was suppressed obviously by CDglyTK gene therapy combined with 3 Gy induction radiation. The expression of CDglyTK gene could be detected by RT-PCR in the transfected tumor, and up-regulation of CDglyTK expression was found in tumor exposed to radiation (P < 0.05). There was significant difference in apoptosis index or proliferation index between tumor without irradiation and tumor with irradiation (P < 0.05).


CONCLUSIONS
The radiation-inducible promoter can be served as a molecular switch to regulate the expression of CDglyTK gene in buccal carcinoma in golden hamster, and low dose induction radiation can significantly improve the therapeutic effects.
Yu
Dong-sheng
DS
Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.
Huang
Hong-zhang
HZ
Hu
Xiao-wen
XW
Liu
Xi-qiang
XQ
Tang
Hai-kuo
HK
Wang
An-xun
AX
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging / Q000628:therapy*; D002610:Cheek / Q000000981:diagnostic imaging*; D006224:Cricetinae; D043525:Cytosine Deaminase / Q000235:genetics; D041722:Genes, Transgenic, Suicide / Q000235:genetics*; D015316:Genetic Therapy / Q000379:methods*; D008647:Mesocricetus; D009062:Mouth Neoplasms / Q000532:radiotherapy / Q000628:therapy*; D011401:Promoter Regions, Genetic / Q000528:radiation effects*; D011859:Radiography; D018139:Simplexvirus / Q000201:enzymology; D013937:Thymidine Kinase / Q000235:genetics
D013937:Thymidine Kinase; D043525:Cytosine Deaminase