Probing biocatalytic transformations with CdSe-ZnS QDs.
CdSe/ZnS QDs enable the optical probing of the biocatalytic oxidation of tyrosine derivatives and of the scission of peptides by thrombin. CdSe/ZnS QDs were modified with tyrosine methyl ester or with a tyrosine-containing peptide. The tyrosine units were reacted with tyrosinase/O2 to yield the respective l-DOPA and quinone derivatives. The luminescence of QDs modified by the enzyme-generated quinone units is quenched. The quinone-functionalized peptide associated with the QDs was cleaved by thrombin, a process that restored the luminescence of the QDs.
Gill
Ron
R
Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Freeman
Ronit
R
Xu
Jian-Ping
JP
Willner
Itamar
I
Winograd
Shira
S
Shweky
Itzik
I
Banin
Uri
U
D015374:Biosensing Techniques; D001711:Biotransformation; D019187:Cadmium Compounds / Q000737:chemistry*; D002384:Catalysis; D005453:Fluorescence; D015394:Molecular Structure; D014442:Monophenol Monooxygenase / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D014443:Tyrosine / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry*
D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D014443:Tyrosine; C058667:cadmium selenide; D014442:Monophenol Monooxygenase; C031238:zinc sulfide; D010100:Oxygen