Microfluidic enzyme-linked immunosorbent assay technology.
In this chapter, we have presented an overview of microfluidic enzyme-linked immunosorbent assay (ELISA) by first introducing the principle of immunoassay, ELISA, and microfabricated devices, followed by a discussion of microfabrication technology and the characterization of microfluidic components. Significant advances in laboratory technology are contributing to the further understanding of microfluidic function, surface modification and immobilization, which lead to the development of improved biomolecule detection methods and prospective applications. For the future, the exploitation of more robust-manufacturing processes and integrated assay systems in an automatic fashion with much reduced assay time and reagent consumption will allow for the effective detection and quantification of biological agents that are of interest in medical diagnostics, food safety surveillance, and environmental monitoring.
Lee
L James
LJ
Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio, USA.
Yang
Shang-Tiang
ST
Lai
Siyi
S
Bai
Yunling
Y
Huang
Wei-Cho
WC
Juang
Yi-Je
YJ
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005517:Foodborne Diseases / Q000175:diagnosis; D015658:HIV Infections / Q000175:diagnosis; D006801:Humans; D044085:Microfluidics; D009369:Neoplasms / Q000175:diagnosis