Characterisation of several heterogeneous species of defective RNAs derived from RNA 3 of cucumber mosaic virus.
Preparations of double-stranded RNAs (dsRNAs) extracted from Nicotiana tabacum cv Xanthi plants infected with a subgroup IB isolate of Cucumber mosaic virus (CMV) were found to contain a heterogeneous population of defective RNAs (D-RNAs) derived from RNA 3. Characterised D-RNAs ranged in size from 1.5 to 1.9 kb and were derived either by a single in-frame deletion within the 3a or 3b genes or by means of double in-frame deletions within both genes. Also, northern blot hybridisation showed two other types of RNA derived from RNA 3: (a) RNA species of ca. 0.7 kb containing the 3'-terminus but lacking the 5'-terminus, which could be 3'-coterminal subgenomic of D-RNAs derived from the 3b gene and (b) RNA species of unknown origin of ca. 0.8 kb containing the 5'-terminus but lacking the 3'-terminus.
López
C
C
Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universidad Politécnica de Valencia (COMAV-UPV), Valencia, Spain. clopez@upvnet.upv.es
Aramburu
J
J
Galipienso
L
L
Nuez
F
F
D001483:Base Sequence; D017799:Cucumovirus / Q000235:genetics*; D017931:DNA Primers; D003673:Defective Viruses / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D012330:RNA, Double-Stranded; D012367:RNA, Viral