title: Detection of Rickettsia rickettsii and Rickettsia sp. in blood clots in 24 patients from different municipalities of the State of Sao Paulo, Brazil.
abstract: The authors detected Rickettsia genus organisms using shell vial and polymerase chain reaction (PCR)/sequencing analysis in blood clots in patients suspected of having Brazilian spotted fever (BSF). DNA was detected using PCR with three sets of primers to access the gltA, ompA, and ompB genes. Sequence analysis was carried out using an automatic sequencer with Bioedit software. Seventy-five percent of the culture samples were positive and all samples amplified rickettsial gene fragments. To date, 46% of the samples have been sequenced.
authors.affiliation: Instituto de Ciências Biomédicas II, Av. Prof. Lineu Prestes, 1374, Cidade Universtitária, 05508-900 São Paulo, Brazil. fgehrke@usp.br
authors.forename: Flávia Sousa
authors.initials: FS
authors.lastname: Gehrke
authors.forename: Elvira Maria
authors.initials: EM
authors.lastname: Mendes do Nascimento
authors.forename: Eliana
authors.initials: E
authors.lastname: Rodrigues de Souza
authors.forename: Silvia
authors.initials: S
authors.lastname: Colombo
authors.forename: Luiz
authors.initials: L
authors.lastname: Jacintho da Silva
authors.forename: Teresinha Tizu Sato
authors.initials: TT
authors.lastname: Schumaker
mesh_terms: D001777:Blood Coagulation; D001938:Brazil / Q000453:epidemiology; D017931:DNA Primers; D006801:Humans; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012281:Rickettsia / Q000235:genetics / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000097:blood / Q000453:epidemiology*; D012284:Rickettsia rickettsii / Q000235:genetics / Q000302:isolation & purification*; D012373:Rocky Mountain Spotted Fever / Q000097:blood / Q000453:epidemiology
chemical_list: D017931:DNA Primers