title: A simple and inexpensive method for investigating microbiological, enzymatic, or inorganic catalysis using standard histology and microbiology laboratory equipment: assembly, mass transfer properties, hydrodynamic conditions and evaluation.
abstract: We introduce a generic, simple, and inexpensive method for performing microbiological, enzymatic, or inorganic catalysis with solids using standard histology and microbiology laboratory equipment. Histology cassettes were used to standardize hydrodynamic conditions and to protect the catalysts and their solid supports. Histology cassettes have the following advantages: they are readily available, inexpensive, solvent and acid resistant, automatable, and the slots in the cassette walls allow liquid to circulate freely. Standard Erlenmeyer flasks were used as reaction vessels. We developed a new camera to observe the movement and position of the histology cassettes as well as the liquid in the Erlenmeyer flasks. The camera produces a stable image of the rotating liquid in the Erlenmeyer flask. This visualization method revealed that in a 250 ml Erlenmeyer flask, stable operating conditions are achieved at a shaking frequency of 300 rpm and a fill volume of 30 ml. In vessels with vertical walls, such as beakers or laboratory bottles, the movement of the histology cassette is not reproducible. Mass transfer characterization using a biological model system and the chemical sulfite-oxidation method revealed that the histology cassette does not influence gas-liquid mass transfer.
authors.affiliation: Biochemical Engineering, RWTH Aachen University, Sammelbau Biologie, Worringerweg 1, 52056, Aachen, Germany. seletzky@biovt.rwth-aachen.de
authors.forename: J M
authors.initials: JM
authors.lastname: Seletzky
authors.forename: K
authors.initials: K
authors.lastname: Otten
authors.forename: S
authors.initials: S
authors.lastname: Lotter
authors.forename: J
authors.initials: J
authors.lastname: Fricke
authors.forename: C P
authors.initials: CP
authors.lastname: Peter
authors.forename: H R
authors.initials: HR
authors.lastname: Maier
authors.forename: J
authors.initials: J
authors.lastname: Büchs
mesh_terms: D002384:Catalysis; D048230:Corynebacterium glutamicum / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry*; D006652:Histological Techniques / Q000295:instrumentation*; D007091:Image Processing, Computer-Assisted; D007287:Inorganic Chemicals / Q000737:chemistry*; D019563:Mechanics; D008828:Microbiological Techniques / Q000295:instrumentation* / Q000379:methods; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D012212:Rheology; D013447:Sulfites / Q000737:chemistry; D014741:Video Recording
chemical_list: D004798:Enzymes; D007287:Inorganic Chemicals; D013447:Sulfites; D010100:Oxygen