[The influence of chronic and of acute irradiation on the polymorphism of RAPD-markers in offspring for irradiated mice].
On mice lines BALB/c and CBA/lac was performed the study of molecular-genetics effects in mice progeny after the chronic (dose rate -0.0017 Gy/day, total dose -0.36 Gy) and acute (dose range 1-3 Gy) exposure of y-radiation on the parents. For variability analysis was used technique of amplification DNA with series of random primers (RAPD-assay). Random primers were used as single primer and in mixture of ones. In this work were held the comparative analysis of the genetic radiosensitivity for stem spermatogonia and spermatides. After the acute exposure the dose dependence for levels of polymorphism of RAPD-markers were obtained. After the chronic irradiation, significant differences from control group were obtained only by use primers mixture M1. Comparative analysis of the genetic radiosensitivity of different stages of mice spermatogenesis are display is similar sensitivity of stem spermatogonia and spermatides after doses of irradiation 1 Gy and 3 Gy. Indicated that after irradiation by dose 2 Gy, spermatogonia are more sensitivity than spermatides.
Miazin
A E
AE
Pomerantseva
M D
MD
Ramaĭia
L K
LK
Shaĭkhaev
G O
GO
Shevchenko
V A
VA
D000818:Animals; D000831:Animals, Newborn; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D005819:Genetic Markers / Q000235:genetics / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D011110:Polymorphism, Genetic; D011836:Radiation Tolerance / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D013087:Spermatids / Q000528:radiation effects; D013091:Spermatogenesis / Q000187:drug effects* / Q000235:genetics; D013093:Spermatogonia / Q000528:radiation effects
D005819:Genetic Markers