title: Digital autofocus methods for automated microscopy.
abstract: Automatic focusing of microscope images is an essential part of modern high-throughput microscopy. This chapter describes implementation of a robust autofocus system appropriate for using either air or oil immersion objectives in robotic imaging. Both hardware and software algorithms are described, and caveats of using viscous immersion media with multifield scanning are detailed.
authors.affiliation: Department of Pharmacology, Linebarger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Feimo
authors.initials: F
authors.lastname: Shen
authors.forename: Louis
authors.initials: L
authors.lastname: Hodgson
authors.forename: Klaus
authors.initials: K
authors.lastname: Hahn
mesh_terms: D000465:Algorithms; D001331:Automation; D019295:Computational Biology / Q000379:methods; D003201:Computers; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D015233:Models, Statistical; D011381:Programming Languages; D012984:Software