[Dosage and kinetics of MMC release of a collagen implant used as a delivery device in glaucoma surgery in the rabbit eye].
OBJECTIVE
To determine the absorption and the release of mitomycin-C from a collagen implant and tissue impregnation in the anterior structures of the rabbit eye.


METHODS
Determining the quantity of mitomycin-C that a collagen implant can absorb with the difference between dry and soaked weight. Mitomycin-C release was measured in vitro using spectrophotometry. The measurement was repeated using a bioassay. Ocular tissue impregnation was determined in 12 eyes of six rabbits. Sclera, implant, aqueous, and ciliary body specimens were collected for concentration measurement using HPLC from 1 to 6 h after surgery.


RESULTS
The mean mitomycin-C quantity absorbed in the implant was 3.22+/-0.2 microg. In vitro release was 0.13 mg/ml after 10 min and 0.05 microg/ml at 6 h. The bioassay showed almost no antifibrotic activity in sclera. In vivo release of mitomycin-C was high from the first to the sixth hour.


CONCLUSIONS
After filtering surgery, mitomycin-C in the collagen implant is clearly released and ocular tissues are effectively impregnated.
Adjadj
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E
Hôpital Ophtalmique Jules Gonin, Université de Lausanne, Suisse. elias_adjadj@yahoo.fr
Roy
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S
Zimmermann
C
C
Shaarawy
T
T
Flammer
J
J
Mermoud
A
A
Drewe
J
J
D000818:Animals; D002921:Cicatrix / Q000517:prevention & control; D003094:Collagen; D016503:Drug Delivery Systems; D018463:Filtering Surgery / Q000009:adverse effects; D005901:Glaucoma / Q000601:surgery*; D016685:Mitomycin / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D019736:Prostheses and Implants; D011817:Rabbits
D016685:Mitomycin; D003094:Collagen