Historical development of analysing large-scale changes in the human genome.
A widely held belief today is that genomics really only started with the DNA sequence information emanating from the genome programs for various organisms, with the human genome playing the leading role. In fact there is a discernable trail stretching for more than a 100 years from the observations of Boveri on tissue instability involving polyploidy in sea urchin embryos and human tumours to the present day. This historical review follows that trail and shows that many theoretical and technical advantages taken for granted in today's genomics era rely heavily on earlier cytogenetic and gene mapping discoveries. Three specific examples of technical developmental paths involving in situ hybridisation, flow-sorting and DNA reassociation kinetics will be explored. In the mid-1980s the two former approaches merged to give rise to several applications of which chromosome painting and chromosome CGH are arguably the most important. The latter developed into array CGH which has now become the pre-eminent method for detecting micro-imbalances in a large number of targets. A competing emerging technology is that of genome-wide SNP typing, which itself is a product of the much earlier RFLP approach linked to DNA sequence information. Do such approaches spell the final demise of the microscope? Perhaps for narrowly defined activities this may occur, but for addressing general questions, microscopic examination will remain pre-eminent.
Pearson
P L
PL
Department of Genetics and Evolutionary Biology, University of Sao Paulo, Brazil. peterlpearson@uol.com.br
D005821:Genetic Techniques; D015894:Genome, Human; D023281:Genomics / Q000266:history*; D049673:History, 20th Century; D006801:Humans; D009693:Nucleic Acid Hybridization; D012106:Research / Q000639:trends; D017422:Sequence Analysis, DNA