title: The use of Raman spectroscopy to provide an estimation of the gross biochemistry associated with urological pathologies.
abstract: Near-infrared Raman spectroscopy, an optical technique that is able to interrogate biological tissues, has been used to study bladder and prostate tissues, with the objective being to provide a first approximation of gross biochemical changes associated with the process of carcinogenesis. Prostate samples for this study were obtained by taking a chip at TURP, and bladder samples from a biopsy taken at TURBT and TURP, following ethical approval. Spectra were taken from purchased biochemical constituents and different pathologies within the bladder and the prostate. We were then able to determine the biochemical basis for these pathologies by utilising an ordinary least-squares fit. We have shown for the first time that we are able to utilise Raman spectroscopy in determining the biochemical basis for the different pathologies within the bladder and prostate gland. In this way we can achieve a better understanding of disease processes such as carcinogenesis. This could have major implications in the future of the diagnosis of disease within the bladder and the prostate gland.
authors.affiliation: Biophotonics Research Group, Pullman Court, Gloucestershire Royal Hospital, Great Western Road, Gloucester GL1 3NN, UK. n.stone@medical-research-centre.com
authors.forename: Nicholas
authors.initials: N
authors.lastname: Stone
authors.forename: Maria Consuelo
authors.initials: MC
authors.lastname: Hart Prieto
authors.forename: Paul
authors.initials: P
authors.lastname: Crow
authors.forename: Jeremy
authors.initials: J
authors.lastname: Uff
authors.forename: Alistair William
authors.initials: AW
authors.lastname: Ritchie
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D003936:Diagnosis, Computer-Assisted / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis*; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D013059:Spectrum Analysis, Raman / Q000379:methods; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D014571:Urologic Neoplasms / Q000175:diagnosis / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins