The effect of Na+-K+ ATPase inhibition by ouabain on histamine release from human cutaneous mast cells.
BACKGROUND
There are controversial reports on the effect of sodium-potassium adenosine triphosphatase (Na+-K+ ATPase) inhibition on mast cell mediator release. Some of them have indicated that ouabain (strophanthin G), a specific Na+-K+ ATPase inhibitor, inhibited the release, whereas the others have shown that ouabain had no effect or even had a stimulatory effect on the mediator secretion. Most of these studies have utilized animal-derived mast cells. The aim of this study was to determine the effect of Na+-K+ ATPase inhibition on human skin mast cells.


METHODS
Unpurified and purified mast cells were obtained from newborn foreskins and stimulated by calcium ionophore A23187 (1 microM) for 30 min following a 1 hr incubation with various concentrations (10(-4) to 10(-8) M) of ouabain. Histamine release was assayed by enzyme-linked immunosorbent assay (ELISA).


RESULTS
The results indicated that ouabain had no significant effect on the non-immunologic histamine release from human skin mast cells, in vitro.


CONCLUSIONS
Na+-K+ ATPase inhibition by ouabain had no significant effect on the non-immunologic histamine release from human cutaneous mast cells and suggested differences between human and animal mast cells.
Senol
Mustafa
M
Department of Dermatology, Inonu University School of Medicine, 44069, Malatya, Turkey. mstfsenol@hotmail.com
Ozerol
I Halil
IH
Patel
Asha V
AV
Skoner
David P
DP
D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D052816:Foreskin / Q000166:cytology; D006632:Histamine / Q000032:analysis; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D010042:Ouabain; D006632:Histamine; D000254:Sodium-Potassium-Exchanging ATPase