title: Time-resolved responses to chemoattractant, characteristic of the front and tail of Dictyostelium cells.
abstract: In a gradient of chemoattractant, Dictyostelium cells are orientated with their front directed toward the source and their tail pointing into the opposite direction. The front region is specified by the polymerization of actin and the tail by the recruitment of filamentous myosin-II. We have dissected these front and tail responses by exposing cells to an upshift of cyclic AMP. A sharp rise and fall of polymerized actin within 10s is accompanied by the recruitment of proteins involved in turning actin polymerization on or off. The cortical accumulation of myosin-II starts when the front response has declined, supporting the concept of divergent signal transmission and adaptation pathways.
authors.affiliation: Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, D-82152 Martinsried, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Etzrodt
authors.forename: Hellen C F
authors.initials: HC
authors.lastname: Ishikawa
authors.forename: Jeremie
authors.initials: J
authors.lastname: Dalous
authors.forename: Annette
authors.initials: A
authors.lastname: Müller-Taubenberger
authors.forename: Till
authors.initials: T
authors.lastname: Bretschneider
authors.forename: Günther
authors.initials: G
authors.lastname: Gerisch
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000818:Animals; D016764:Cell Polarity / Q000187:drug effects*; D002630:Chemotactic Factors / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology; D004023:Dictyostelium / Q000166:cytology* / Q000187:drug effects*; D009218:Myosins / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D015800:Protozoan Proteins / Q000378:metabolism; D013997:Time Factors; D054378:Acetogenins
chemical_list: D015107:4-Butyrolactone; D000199:Actins; D002630:Chemotactic Factors; D000242:Cyclic AMP; D009218:Myosins; D015800:Protozoan Proteins; C438920:coronin acetogenin; D054378:Acetogenins