title: Intrathecal administration of Cav3.2 and Cav3.3 antisense oligonucleotide reverses tactile allodynia and thermal hyperalgesia in rats following chronic compression of dorsal root of ganglion.
abstract: OBJECTIVE
  The present study aimed to elucidate the role of T-subtype calcium channels (Cav3.1, Cav3.2, and Cav3.3) in the pathogenesis of neuropathic pain at spinal level.
  
  
  METHODS
  The chronic compression of the dorsal root ganglion (CCD) rat model was adopted. The antisense oligonucleotide of Cav3.1, Cav3.2, and Cav3.3 or normal saline (NS) were intrathecally administered twice per day from the first day to the fourth day after operation. Paw mechanical withdrawal threshold and paw thermal withdrawal latency were measured to evaluate the tactile allodynia and thermal hyperalgesia, respectively.
  
  
  RESULTS
  CCD rats developed reliable tactile allodynia and thermal hyperalgesia after operation. Intrathecal administration of antisense oligonucleotide of Cav3.2 and Cav3.3 significantly relieved tactile allodynia and thermal hyperalgesia in CCD rats, but not Cav3.1.
  
  
  CONCLUSIONS
  Cav3.2 and Cav3.3 subtype calcium channels in the spinal cord may play an important role in the pathogenesis of neuropathic pain, which may contribute to the management of the neuropathic pain.
authors.affiliation: Department of Anesthesiology, the First Peopleos Hospital of FoShan and Affiliated FoShan Hospital of Sun-Yat Sen University, Foshan 528000, China.
authors.forename: Xian-jie
authors.initials: XJ
authors.lastname: Wen
authors.forename: Zhang-jun
authors.initials: ZJ
authors.lastname: Li
authors.forename: Zhi-xin
authors.initials: ZX
authors.lastname: Chen
authors.forename: Zhi-yuan
authors.initials: ZY
authors.lastname: Fang
authors.forename: Chen-xiang
authors.initials: CX
authors.lastname: Yang
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Yin-ming
authors.initials: YM
authors.lastname: Zeng
mesh_terms: D000818:Animals; D020747:Calcium Channels, T-Type / Q000235:genetics* / Q000378:metabolism; D005727:Ganglia, Spinal; D006358:Hot Temperature; D006930:Hyperalgesia / Q000503:physiopathology*; D007278:Injections, Spinal; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D009408:Nerve Compression Syndromes / Q000378:metabolism / Q000503:physiopathology*; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage / Q000494:pharmacology*; D010146:Pain / Q000503:physiopathology*; D010147:Pain Measurement / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism
chemical_list: C469757:CACNA1I protein, human; C509958:Cacna1h protein, rat; D020747:Calcium Channels, T-Type; D026901:Membrane Transport Proteins; D016376:Oligonucleotides, Antisense