Signature-tagged mutagenesis: barcoding mutants for genome-wide screens.
DNA signature tags (molecular barcodes) facilitate functional screens by identifying mutants in mixed populations that have a reduced or increased adaptation to a particular environment. Many innovative adaptations and refinements in the technology have been described since its original use with Salmonella; they have yielded a wealth of information on a broad range of biological processes--mainly in bacteria, but also in yeast and other fungi, viruses, parasites and, most recently, in mammalian cells. By combining whole-genome microarrays and comprehensive ordered libraries of mutants, high-throughput functional screens can now be achieved on a genomic scale.
Mazurkiewicz
Piotr
P
Department of Infectious Diseases, Centre for Molecular Microbiology and Infection, Imperial College London, Armstrong Road, London SW7 2AZ, UK.
Tang
Christoph M
CM
Boone
Charles
C
Holden
David W
DW
D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000254:growth & development; D015723:Gene Library; D016678:Genome; D006801:Humans; D016254:Mutagenesis, Insertional; D009154:Mutation / Q000235:genetics*; D034622:RNA Interference; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development