title: Penetration of beta-lactoglobulin into monoglyceride monolayers. dynamics, interactions, and topography of mixed films.
abstract: In this work we have analyzed the penetration of betalactoglobulin into a monoglyceride monolayer (monopalmitin or monoolein) spread at the air-water interface and its effects on the structural, dilatational, and topographical characteristics of mixed films. Dynamic tensiometry, surface film balance, Brewster angle microscopy (BAM), and surface dilatational rheology have been used, maintaining the temperature constant at 20 degrees C and the pH and ionic strength at 7 and 0.05 M, respectively. The initial surface pressure (mN/m) of the spread monoglyceride monolayer (pii(MONOGLYCERIDE)) at 10, 20, and the collapse point is the variable studied. Beta-lactoglobulin can penetrate into a spread monoglyceride monolayer at every surface pressure. The penetration of beta-lactoglobulin into the monoglyceride monolayer with a more condensed structure, at the collapse point of the monoglyceride, requires monoglyceride molecular loss by collapse and/or desorption. However, the structural, topographical, and dilatational characteristics of monoglyceride penetrated by beta-lactoglobulin mixed monolayers are essentially dominated by the presence of monoglyceride (either monopalmitin or monoolein) in the mixed film. In fact, monoglyceride molecules have the capacity to re-enter the monolayer after expansion and recompression of the mixed monolayer. Thus, monoglyceride molecular loss by collapse and/or desorption is reversible. The topography of the monolayer under dynamic conditions corroborates these conclusions.
authors.affiliation: Departamento de Ingeniería Química, Facultad de Química, Universidad de Sevilla, C/. Prof. García González 1, 41012-Seville, Spain.
authors.forename: Marta Cejudo
authors.initials: MC
authors.lastname: Fernández
authors.forename: Cecilio Carrera
authors.initials: CC
authors.lastname: Sánchez
authors.forename: Rosario Rodríguez
authors.initials: RR
authors.lastname: Niño
authors.forename: Juan M Rodríguez
authors.initials: JM
authors.lastname: Patino
mesh_terms: D000388:Air; D005989:Glycerides / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007782:Lactoglobulins / Q000737:chemistry*; D008853:Microscopy; D008958:Models, Molecular; D050178:Monoglycerides / Q000737:chemistry*; D012212:Rheology; D013499:Surface Properties; D013696:Temperature; D014867:Water
chemical_list: D005989:Glycerides; D007782:Lactoglobulins; D050178:Monoglycerides; D014867:Water; C005953:monoolein; C027735:palmitoyl glycerol