iNOs expression is stimulated by the major surface protein (rWSP) from Wolbachia bacterial endosymbiont of Dirofilaria immitis following subcutaneous injection in mice.
The bacterial endosymbiont Wolbachia of several species of filarial nematodes plays an important role in the inflammatory pathology of filariasis. Nitric oxide (NO) production has also been implicated in the immune response during filarial infections. Here we present data indicating that a recombinant Wolbachia surface protein (rWSP) induces iNOs mRNA expression and NO production, as well as IFN-gamma and a Th1-type antibody response, in inoculated BALB/c mice. This effect is not observed when mice are inoculated with a recombinant heat shock protein from Wolbachia (GroEL).
Morchón
R
R
Laboratorio de Parasitologia, Universidad de Salamanca, Salamanca, Spain.
Bazzocchi
C
C
López-Belmonte
J
J
Martín-Pacho
J R
JR
Kramer
L H
LH
Grandi
G
G
Simón
F
F
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D018834:Chaperonin 60 / Q000276:immunology; D004183:Dirofilaria immitis / Q000382:microbiology* / Q000502:physiology; D004184:Dirofilariasis / Q000276:immunology / Q000469:parasitology; D007249:Inflammation / Q000276:immunology; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D016753:Interleukin-10 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger; D011994:Recombinant Proteins / Q000276:immunology; D013559:Symbiosis; D018417:Th1 Cells / Q000276:immunology; D020577:Wolbachia / Q000276:immunology* / Q000502:physiology
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D018834:Chaperonin 60; D012333:RNA, Messenger; D011994:Recombinant Proteins; C112171:Wsp protein, Wolbachia; D016753:Interleukin-10; D015847:Interleukin-4; D009569:Nitric Oxide; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse