title: Genetic resistance to Sarcocystis miescheriana in pigs following experimental infection.
abstract: Clinical and parasitological traits of Sarcocystis miescheriana differ in Pietrain and Meishan pigs. For further description and characterization of the genetic basis of this variation a F(2) family based on Pietrain boars and Meishan sows as founders was generated. One hundred and thirty-nine F(2) pigs were challenged orally at an age of 100 days with 50,000 sporozysts to produce the typical clinical picture of a moderate dose Sarcocystis infection. Heritabilities were estimated for clinical and clinical-chemical traits, for specific antibody responses to the infection and for bradyzoite numbers found in skeletal (Musculus longissimus dorsi: M.l.d.) and heart muscles at necropsy 70 days post-infection (p.i.) Apart from several low to moderate heritabilities, high heritabilities were observed for bradyzoite numbers in the M.l.d. (0.68), IgM antibody levels (0.74) during the acute (14 days p.i.) and titres of specific IgG antibodies (0.42) in the early stage of cyst formation (42 days p.i.). Marked heritabilities of these traits, which are basic for acute phase of the disease (14 days p.i.) or chronic Sarcocystosis presume genes that explain sufficient shares of variance (QTL). The model is considered valuable for screening of gene variants associated with resistance/susceptibility to Sarcocystis infection. Such gene variants could then be used in susceptibility-scoring or selection programs in the future.
authors.affiliation: Department of Swine Diseases, University of Giessen, D-35392 Giessen, Germany. gerald.reiner@vetmed.uni-giessen.de
authors.forename: G
authors.initials: G
authors.lastname: Reiner
authors.forename: S
authors.initials: S
authors.lastname: Hepp
authors.forename: B
authors.initials: B
authors.lastname: Hertrampf
authors.forename: D
authors.initials: D
authors.lastname: Kliemt
authors.forename: U
authors.initials: U
authors.lastname: Mackenstedt
authors.forename: A
authors.initials: A
authors.lastname: Daugschies
authors.forename: H
authors.initials: H
authors.lastname: Zahner
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D007074:Immunoglobulin G / Q000097:blood / Q000235:genetics; D007075:Immunoglobulin M / Q000097:blood / Q000235:genetics; D008297:Male; D012522:Sarcocystis / Q000502:physiology*; D012523:Sarcocystosis / Q000235:genetics / Q000469:parasitology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000235:genetics* / Q000469:parasitology*
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M