title: Diallyl sulfide induces the expression of nucleotide excision repair enzymes in the breast of female ACI rats.
abstract: Diethylstilbestrol (DES) causes DNA adducts resulting in breast cancer, whereas diallyl sulfide (DAS) inhibits cancer formation. We hypothesize that DAS induces the expression of nucleotide excision repair genes. To test this hypothesis, female ACI rats were treated for 4 days with corn oil, DES, DAS, and DAS/DES (50mg/kg). The expression of P53, Gadd45a, PCNA, and DNA polymerase delta was analyzed by real-time PCR. DES decreased the expression of P53, Gadd45a and PCNA. DAS and DAS/DES increased the expression of all four genes. These results suggest that DAS enhances the ability of breast tissue to repair DNA damage thus preventing cancer.
authors.affiliation: College of Pharmacy, Florida A&M University, 302 West Pershing Street, Tallahassee, FL 32307, United States. mgreen006@hotmail.com
authors.forename: Mario
authors.initials: M
authors.lastname: Green
authors.forename: Oneil
authors.initials: O
authors.lastname: Newell
authors.forename: Ayoola
authors.initials: A
authors.lastname: Aboyade-Cole
authors.forename: Selina
authors.initials: S
authors.lastname: Darling-Reed
authors.forename: Ronald D
authors.initials: RD
authors.lastname: Thomas
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001940:Breast / Q000187:drug effects* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics; D004258:DNA Polymerase III / Q000235:genetics; D004260:DNA Repair; D004054:Diethylstilbestrol / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D009687:Nuclear Proteins / Q000235:genetics; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D051381:Rats; D011912:Rats, Inbred ACI; D013440:Sulfides / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D000097642:GADD45 Proteins
chemical_list: D000498:Allyl Compounds; D016588:Anticarcinogenic Agents; D018797:Cell Cycle Proteins; D004258:DNA Polymerase III; D004054:Diethylstilbestrol; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D013440:Sulfides; D016159:Tumor Suppressor Protein p53; C499073:Gadd45a protein, rat; D000097642:GADD45 Proteins; C038491:allyl sulfide