title: Combining stir bar sorptive extraction and MEKC for the determination of polynuclear aromatic hydrocarbons in environmental and biological matrices.
abstract: In this work, stir bar sorptive extraction and liquid desorption was combined with MEKC and diode-array detection (SBSE-LD-MEKC-DAD) for the determination of polynuclear aromatic hydrocarbons (PAHs) in aqueous medium, using biphenyl, fluorene, anthracene, phenanthrene, fluoranthene and pyrene as model compounds. MEKC-DAD conditions and parameters affecting SBSE-LD efficiency are fully discussed. Assays performed on aqueous samples spiked at trace levels, yielded recoveries ranging from 55.5 +/- 6.1% (pyrene) to 70.7 +/- 7.1% (anthracene), under optimized experimental conditions. The methodology proved to be nearly described by the octanol-water partition coefficients (K(PDMS/W) approximately K(O/W)). The analytical performance showed good precision (<12.0%), suitable detection limits (2-11 microg/L) and convenient linear dynamic ranges (r(2)>0.99) from 5 to 25 microg/L for anthracene and 25 to 125 microg/L for the remaining compounds. The application of the proposed methodology to environmental water, sediments and fish bile matrices demonstrated good selectivity and accuracy. SBSE-LD combined with MEKC-DAD was shown to be an easy, reliable and robustness methodology, as well as a good analytical alternative to monitor environmental priority pollutants.
authors.affiliation: Chemistry and Biochemistry Department, University of Lisbon, Lisbon, Portugal.
authors.forename: Pedro M A
authors.initials: PM
authors.lastname: do Rosário
authors.forename: José M F
authors.initials: JM
authors.lastname: Nogueira
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry; D012634:Bodily Secretions / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary; D005399:Fishes; D019015:Geologic Sediments / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000379:methods*; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D014867:Water