title: Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus.
abstract: We recently mapped Idd4 to a 5.2 cM interval on chromosome 11 with two subloci, Idd4.1 and Idd4.2, in nonobese diabetic (NOD) mice. Based on the localization of platelet-activating factor acetylhydrolase Ib1 (PAF-AHIb1) and the decreased activity of PAF-AH in type 1 diabetes (T1D) patients, we hypothesized that PAF-AHIb1 in Idd4.1 is a candidate gene. The PAF-AHIb1 gene in NOD mice was cloned and sequenced, and its expression and function were studied. No polymorphisms were detected in PAF-AHIb1 cDNA between NOD and B6 mice. The expression of PAF-AH Ib1 at the mRNA and protein levels was found to be similar in different tissues between NOD and B6 mice. PAF-AH activity does not differ in the pancreatic islets or spleen between NOD and B6 mice. Our findings suggest that PAF-AH Ib1 may not be a diabetes-susceptibility gene in the Idd4.1 sublocus.
authors.affiliation: Laboratory of Autoimmune Diabetes, Robarts Research Institute, 100 Perth Drive, London, Ontario, Canada. del@robarts.ca
authors.forename: Qing-Sheng
authors.initials: QS
authors.lastname: Mi
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Marsha
authors.initials: M
authors.lastname: Grattan
authors.forename: Zai-Zhao
authors.initials: ZZ
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Sivilotti
authors.forename: Jing-Xiang
authors.initials: JX
authors.lastname: She
authors.forename: Terry L
authors.initials: TL
authors.lastname: Delovitch
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology / Q000503:physiopathology*; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D051379:Mice; D016688:Mice, Inbred NOD; D008817:Mice, Mutant Strains; D016513:Mice, SCID
chemical_list: D005819:Genetic Markers; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase