title: Rapid and efficient MALDI-TOF MS peak detection of 2-nitrobenzenesulfenyl-labeled peptides using the combination of HPLC and an automatic spotting apparatus.
abstract: In this paper, we report MALDI-TOF ms analysis of 2-nitrobenzenesulfenyl (NBS) labeled peptides with the powerful aid of an LC-automatic spotting system. using this approach we analyzed mammalian sera (rat and mouse) as biological samples to demonstrate performance. The labeling was carried out using a binary set of 2-nitrobenzenesulfenyl chloride (heavy and light), which modified tryptophan residues in sample proteins. Approximately 1600 doublet peaks were detected in the mass spectrum, some of which had more than threefold differences in their intensities. systematic separation/spotting followed by mass analysis of the NBS-labeled peptides derived from biological samples is described for the first time. This method has proved to be an effective application of NBS-labeled peptides and can be a powerful technique for quantitative analysis of proteins expressed in biological systems.
authors.affiliation: Life Science Laboratory, Shimadzu Corporation, Kyoto, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Iida
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kuyama
authors.forename: Makoto
authors.initials: M
authors.lastname: Watanabe
authors.forename: Chikako
authors.initials: C
authors.lastname: Toda
authors.forename: Ei-ichi
authors.initials: E
authors.lastname: Matsuo
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kido
authors.forename: Eiji
authors.initials: E
authors.lastname: Ando
authors.forename: Susumu
authors.initials: S
authors.lastname: Tsunasawa
authors.forename: Osamu
authors.initials: O
authors.lastname: Nishimura
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D009578:Nitrobenzenes; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009578:Nitrobenzenes; D010446:Peptide Fragments; C002732:2-nitrobenzenesulfenyl chloride