title: Prevalence of human cytomegalovirus UL97 D605E mutation in transplant recipients in China.
abstract: Human cytomegalovirus (CMV) resistance to gancyclovir (GCV) occurs via mutation in the UL97 gene, ethylene diamine tetraacetic acid blood samples were obtained from 23 transplant recipients who received a GCV implant. A nested polymerase chain reaction amplifying UL97 codons 450 to 672 was performed. Nested amplifications were sequenced directly. No known UL97 GCV resistance mutations were found. Eighteen of 23 patients (78%) had revealed mutations at codon 605 (D to E). Mutant D605E may reverse to wild-type during the follow-up treatment. We conclude that human CMV UL97 D605E mutation occurred in Chinese transplant recipients. This mutation may be regarded as a natural sequence variant.
authors.affiliation: The First Affiliated Hospital of Zhejiang University School of Medicine, Key Lab of Combined Multi-organ Transplantation, Hangzhou, Zhejiang, China. linzhou8@mail.hz.zj.cn
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: J
authors.initials: J
authors.lastname: Fan
authors.forename: S S
authors.initials: SS
authors.lastname: Zheng
authors.forename: W H
authors.initials: WH
authors.lastname: Ma
mesh_terms: D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D002681:China; D003062:Codon; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000453:epidemiology / Q000821:virology; D017931:DNA Primers; D005784:Gene Amplification; D014644:Genetic Variation; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D009154:Mutation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D011183:Postoperative Complications / Q000453:epidemiology / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D003062:Codon; D017931:DNA Primers; D017853:Phosphotransferases (Alcohol Group Acceptor); C075365:ganciclovir kinase