Is there any association between nek3 and cancers with frequent 13q14 deletion?
Chromosomal region 13q14 is frequently deleted in prostate cancer. nek3, a protein kinase related gene, is located on this region. Analysis of the coding region of nek3 showed an A insertion/deletion polymorphism in a stretch of adenines at the end of exon 9, with 2 alleles showing either 7 or 8 adenines. In addition we found a variant human NEK3 transcript, which lacks the entire exon 10 due to alternative splicing. The frequency of A8 allele is statistically higher in prostate cancer samples (p < 0.001) than normal controls, indicating that tumor samples preferentially express a full length protein. On the contrary, normal samples have a higher frequency for the A7 allele, expressing preferentially a shorter protein. To test if this association is a common feature in cancers with frequent 13q14 alterations, we analyzed cell lines established from oral, lung, and hepatocellular cancers. An association between nek3 A insertion/deletion polymorphism and cancers with alterations at 13q14 is observed.
Hernández
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M
Universidad de La Laguna, Facultad de Biología, Departamento de Genética, Tenerife, Islas Canarias, Spain.
Almeida
T A
TA
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002872:Chromosome Deletion; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D000072379:NIMA-Related Kinases; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D012333:RNA, Messenger; C486224:NEK3 protein, human; D000072379:NIMA-Related Kinases; D017346:Protein Serine-Threonine Kinases