title: The physical and functional interaction of NDRG2 with MSP58 in cells.
abstract: NDRG2, a member of N-Myc downstream regulated gene family, exerts the important functions in cell differentiation and tumor suppression. Although the ectopic expressed Ndrg2 inhibits the proliferation of tumor cells, its intracellular signal transduction pathway is hardly known. Here, we identified MSP58, a 58-kDa microspherule protein, as an interacting partner of human Ndrg2 by using yeast two-hybrid screening. The interaction was confirmed by glutathione S-transferase pull-down assay in vitro and by co-immune-precipitation assay in vivo. The forkhead associated domain of MSP58 is essential for its interaction with Ndrg2. Ndrg2 could co-localize with MSP58 in nuclear of HeLa cell during cell stress. Furthermore, the modulation of Ndrg2 level influences the cell cycle process together with MSP58. In conclusion, the findings offered a novel insight into the physiological roles of Ndrg2.
authors.affiliation: The Department of Biochemistry and Molecular Biology and The State Key Laboratory of Cancer Biology, The Fourth Military Medical University, Xi'an, China 710032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Junye
authors.initials: J
authors.lastname: Liu
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Fuyang
authors.initials: F
authors.lastname: Li
authors.forename: Lifeng
authors.initials: L
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Xinping
authors.initials: X
authors.lastname: Liu
authors.forename: Lan
authors.initials: L
authors.lastname: Shen
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Deng
authors.forename: Angang
authors.initials: A
authors.lastname: Yang
authors.forename: Hua
authors.initials: H
authors.lastname: Han
authors.forename: Mujun
authors.initials: M
authors.lastname: Zhao
authors.forename: Libo
authors.initials: L
authors.lastname: Yao
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D002522:Chlorocebus aethiops; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins
chemical_list: C113292:MCRS1 protein, human; C466228:NDRG2 protein, human; D009687:Nuclear Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; D025521:Tumor Suppressor Proteins