title: Post TLC developing technique for tyrosinase inhibitor detection.
abstract: Post TLC developing technique was developed to detect substances which can inhibit tyrosinase activity. The method involved spraying the TLC plate or chromatographic paper containing sample spot(s) with tyrosinase and l-tyrosine solutions successively. A positive result could be visualized directly as white spot(s) against a brownish-purple background. The method can either be used as a quick screening method for tyrosinase inhibitor detection or a guiding procedure for an isolation of tyrosinase inhibitors from mixtures or natural product extracts. The technique is sensitive enough to give a clear result in the presence of only 6 ng glabridin.
authors.affiliation: Department of Chemistry, Faculty of Science, Chulalongkorn University, Payatai, Bangkok 10330, Thailand.
authors.forename: Sakulna
authors.initials: S
authors.lastname: Wangthong
authors.forename: Ilhum
authors.initials: I
authors.lastname: Tonsiripakdee
authors.forename: Thitinun
authors.initials: T
authors.lastname: Monhaphol
authors.forename: Ratchanee
authors.initials: R
authors.lastname: Nonthabenjawan
authors.forename: Supason Pattanaargson
authors.initials: SP
authors.lastname: Wanichwecharungruang
mesh_terms: D000363:Agaricales; D002855:Chromatography, Thin Layer / Q000379:methods*; D005656:Fungal Proteins / Q000037:antagonists & inhibitors; D007529:Isoflavones; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D010455:Peptides / Q000032:analysis*; D010636:Phenols / Q000032:analysis; D011753:Pyrones / Q000032:analysis*; D012015:Reference Standards; D012680:Sensitivity and Specificity
chemical_list: D005656:Fungal Proteins; D007529:Isoflavones; D010455:Peptides; D010636:Phenols; D011753:Pyrones; C011890:kojic acid; D014442:Monophenol Monooxygenase; C107601:glabridin