Recombinant galectins of male and female Haemonchus contortus do not hemagglutinate erythrocytes of their natural host.
Recombinant galectins of female and male adult worms of Haemonchus contortus were expressed in Escherichia coli and their hemagglutinating activities to human and different animal erythrocytes were analyzed. The results showed that female and male galectins could be highly expressed in E. coli using a temperature-sensitive plasmid, with the recombinant protein being mainly appeared in inclusion bodies. Hemagglutinating activity assays showed that both of the galectins hemagglutinated human A, B, O type, dog, rabbit, chicken and mouse erythrocytes at the high concentration of 40 microg/well, but did not hemagglutinate erythrocytes of the natural host of H. contortus, the goat. Sugar inhibition assays confirmed that, out of eight sugars tested, only lactose was effective to inhibit agglutination of human type B erythrocytes by the recombinant galectins.
Li
Chunhua
C
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Wei
Xiaofeng
X
Xu
Lixin
L
Li
Xiangrui
X
D000818:Animals; D002241:Carbohydrates; D002645:Chickens / Q000097:blood; D004285:Dogs / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects*; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D037161:Galectins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial; D006041:Goats / Q000097:blood; D006189:Haemonchus / Q000235:genetics / Q000378:metabolism*; D015801:Helminth Proteins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice / Q000097:blood; D020033:Protein Isoforms; D011817:Rabbits / Q000097:blood; D011994:Recombinant Proteins
D002241:Carbohydrates; D037161:Galectins; D015801:Helminth Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins