title: Drosophila homologs of mammalian TNF/TNFR-related molecules regulate segregation of Miranda/Prospero in neuroblasts.
abstract: During neuroblast (NB) divisions, cell fate determinants Prospero (Pros) and Numb, together with their adaptor proteins Miranda (Mira) and Partner of Numb, localize to the basal cell cortex at metaphase and segregate exclusively to the future ganglion mother cells (GMCs) at telophase. In inscuteable mutant NBs, these basal proteins are mislocalized during metaphase. However, during anaphase/telophase, these mutant NBs can partially correct these earlier localization defects and redistribute cell fate determinants as crescents to the region where the future GMC "buds" off. This compensatory mechanism has been referred to as "telophase rescue". We demonstrate that the Drosophila homolog of the mammalian tumor-necrosis factor (TNF) receptor-associated factor (DTRAF1) and Eiger (Egr), the homolog of the mammalian TNF, are required for telophase rescue of Mira/Pros. DTRAF1 localizes as an apical crescent in metaphase NBs and this apical localization requires Bazooka (Baz) and Egr. The Mira/Pros telophase rescue seen in inscuteable mutant NBs requires DTRAF1. Our data suggest that DTRAF1 binds to Baz and acts downstream of Egr in the Mira/Pros telophase rescue pathway.
authors.affiliation: Drosophila Neurobiology Lab, Institute of Molecular and Cell Biology, Proteos Building, 61 Biopolis Drive, Singapore 138673, Singapore.
authors.forename: Huashan
authors.initials: H
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Cai
authors.forename: William
authors.initials: W
authors.lastname: Chia
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D002352:Carrier Proteins; D018797:Cell Cycle Proteins / Q000378:metabolism*; D019070:Cell Lineage; D016764:Cell Polarity; D020090:Chromosome Segregation; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004330:Drosophila / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D007605:Juvenile Hormones / Q000378:metabolism; D008322:Mammals; D008565:Membrane Proteins / Q000378:metabolism; D008677:Metaphase; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000166:cytology*; D009687:Nuclear Proteins / Q000378:metabolism*; D021381:Protein Transport; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D017385:Sequence Homology; D047990:TNF Receptor-Associated Factor 1 / Q000378:metabolism; D013692:Telophase; D014157:Transcription Factors / Q000378:metabolism*; D048069:Tumor Necrosis Factors / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D007605:Juvenile Hormones; D008565:Membrane Proteins; C107613:Mira protein, Drosophila; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C115164:PON protein, Drosophila; D018124:Receptors, Tumor Necrosis Factor; D047990:TNF Receptor-Associated Factor 1; D014157:Transcription Factors; D048069:Tumor Necrosis Factors; C400774:baz protein, Drosophila; C464573:egr protein, Drosophila; C098678:insc protein, Drosophila; C060606:numb protein, Drosophila; C073099:pros protein, Drosophila