Early diagnosis of rickettsioses by electrochemiluminscence.
The diagnosis of acute rickettsioses during the acute phase of the disease is challenging. We present preliminary evidence that antigen-capture using streptavidin-coated magnetic beads with biotinylated anti-rickettsia typhi rabbit polyclonal antibodies followed by electrochemiluminescent detection with ruthenylated antibodies of the same specificity could be used for the diagnosis of rickettsial diseases in the acute phase.
Wen
Gary
G
Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, R. 0609, Galveston, TX 77555-0609, USA.
McBride
Jere W
JW
Zhang
Xiaofeng
X
Olano
Juan P
JP
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000302:isolation & purification; D004563:Electrochemistry / Q000379:methods; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D011817:Rabbits; D012282:Rickettsia Infections / Q000175:diagnosis*; D012286:Rickettsia typhi; D014437:Typhus, Endemic Flea-Borne / Q000175:diagnosis
D000907:Antibodies, Bacterial