title: Endovascular treatment of experimental aneurysms by use of fibroblast-coated platinum coils: an angiographic and histopathologic study.
abstract: OBJECTIVE
  The purpose of this study was to determine whether implanting exogenous fibroblasts on platinum coils could enhance intra-aneurysmal fibrosis. Hypotheses included: (1) fibroblast-coated (FBC) platinum coils can improve angiographic results after embolization; and (2) FBC platinum coils can accelerate histological healing of embolized aneurysms.
  
  
  METHODS
  Experimental aneurysms in rabbits were embolized with control platinum coils (n=18) or FBC coils (n=18). Subjects were euthanized at 14 days, 1 month, 3 months and 6 months after implantation. Digital subtraction angiography was used to evaluate stability after embolization. Histological samples were examined with a grading system (range, 0 to 12) based on neck and dome healing.
  
  
  RESULTS
  Histology total scores and fibrosis ratio at 14 days were significantly greater in the FBC coil group compared with controls (6.6+/-1.9 versus 2.5+/-1.1, 1.2+/-0.6% versus 0.2+/-0.3%, respectively; P=0.0090). Cavities embolized with FBC coils showed cellular proliferation and thrombus organization, with an endothelialized membrane bridging the neck. There were no differences between groups in the later timepoints. The FBC coil group showed radiographic stability in 11 (61%) cases, coil compaction in 2 (11%) cases, and progressive occlusion in 5 (28%) cases. No progressive occlusion was seen in controls; 3 (17%) of 18 control cases exhibited coil compaction (P=0.0546).
  
  
  CONCLUSIONS
  FBC coils can accelerate early histological healing compared with control coils in the rabbit aneurysm model.
authors.affiliation: Neuroradiology Research Laboratory, Department of Radiology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
authors.forename: Daying
authors.initials: D
authors.lastname: Dai
authors.forename: Yong Hong
authors.initials: YH
authors.lastname: Ding
authors.forename: Mark A
authors.initials: MA
authors.lastname: Danielson
authors.forename: Ramanathan
authors.initials: R
authors.lastname: Kadirvel
authors.forename: Larry W
authors.initials: LW
authors.lastname: Hunter
authors.forename: Wen-Zhi
authors.initials: WZ
authors.lastname: Zhan
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Helm
authors.forename: Debra A
authors.initials: DA
authors.lastname: Lewis
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Cloft
authors.forename: Gary C
authors.initials: GC
authors.lastname: Sieck
authors.forename: David F
authors.initials: DF
authors.lastname: Kallmes
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015415:Biomarkers; D002478:Cells, Cultured; D002533:Cerebral Angiography; D004195:Disease Models, Animal; D004621:Embolization, Therapeutic / Q000295:instrumentation* / Q000379:methods*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D002532:Intracranial Aneurysm / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D008297:Male; D032390:Myoblasts, Smooth Muscle; D010984:Platinum / Q000627:therapeutic use; D019736:Prostheses and Implants / Q000592:standards / Q000639:trends*; D011817:Rabbits; D016896:Treatment Outcome; D014746:Vimentin / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology
chemical_list: D000199:Actins; D015415:Biomarkers; D010984:Platinum; D014746:Vimentin; C498862:Acta2 protein, rat