[Effects of huanglian jiedu decoction on signal transduction of insulin receptor and insulin receptor substrate in adipose tissue of insulin resistant rats].
OBJECTIVE
To investigate the effects of Huanglian Jiedu decoction (HLJD) on protein expressions and tyrosine phosphorylation levels of insulin receptor (InsR) and insulin receptor substrate-1 (IRS-1) in adipose tissue of insulin resistant (IR) rats, and explore its possible molecular mechanism in improving IR.


METHODS
IR model was induced by intravenous injection of a small dose of streptozotocin combined with high fat and caloricity diet feeding in Wistar rats. And the model rats in the testing group were treated with HJD for 10 weeks. Fasting serum glucose and insulin were determined, and protein expressions and tyrosine phosphorylation levels of InsR and IRS-1 in adipose tissue of epididymides were detected by immunoprecipitation and Western blot.


RESULTS
The protein expression of IRS-1 and the tyrosine phosphorylation levels of InsR and IRS-1 increased significantly in model rats treated with HLJD, compared with those in the untreated model rats.


CONCLUSIONS
HLJD could increase the tyrosine phosphorylation levels of InsR and IRS-1 in adipose tissue in IR rats, which maybe one of its mechanisms in lowering blood glucose and improving insulin sensitivity of the target tissues.
Ye
Ai-li
AL
Institute of Integrative Traditional Chinese and Western Medicine, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan.
Lu
Fu-er
FE
Xu
Li-jun
LJ
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000378:metabolism / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D047468:Immunoprecipitation; D007328:Insulin / Q000097:blood; D055504:Insulin Receptor Substrate Proteins; D007333:Insulin Resistance; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011972:Receptor, Insulin / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
D048868:Adaptor Proteins, Signal Transducing; D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; C061676:oren gedoku to; D011972:Receptor, Insulin