The human CMV-UL86 peptide 981-1003 shares a crossreactive T-cell epitope with the encephalitogenic MOG peptide 34-56, but lacks the capacity to induce EAE in rhesus monkeys.
Rhesus monkeys immunized with MOG(34-56), a dominant T-cell epitope from myelin/oligodendrocyte glycoprotein, develop an acute neurological disease resembling acute disseminated encephalomyelitis (ADEM) in humans. The typical large demyelinated lesions and mononuclear infiltrates in the monkey brains are caused by MOG(34-56) T-cells. We show that MOG(34-56)-reactive CD4+ and CD8+ T-cells are induced in monkeys immunized with a peptide from the human CMV major capsid protein (UL86; 981-1003), that shares sequence similarity with MOG(34-56). Monkeys sensitized against the viral peptide and subsequently challenged with MOG(34-56) display histological signs of encephalitis, but do not show overt neurological signs.
Brok
Herbert P M
HP
Department of Immunobiology, Biomedical Primate Research Centre, 2288 GJ Rijswijk, The Netherlands.
Boven
Leonie
L
van Meurs
Marjan
M
Kerlero de Rosbo
Nicole
N
Celebi-Paul
Liesbeth
L
Kap
Yolanda S
YS
Jagessar
Anwar
A
Hintzen
Rogier Q
RQ
Keir
Geoff
G
Bajramovic
Jeffrey
J
Ben-Nun
Avraham
A
Bauer
Jan
J
Laman
Jon D
JD
Amor
Sandra
S
't Hart
Bert A
BA
D000818:Animals; D000906:Antibodies / Q000097:blood; D001921:Brain / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology* / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000276:immunology; D003429:Cross Reactions; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D008253:Macaca mulatta / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000473:pathology; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000276:immunology / Q000494:pharmacology; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments / Q000276:immunology* / Q000494:pharmacology; D011092:Polyethylene Glycols; D011994:Recombinant Proteins / Q000276:immunology; D013116:Spinal Cord / Q000473:pathology
D000906:Antibodies; D036022:Capsid Proteins; D018984:Epitopes, T-Lymphocyte; D006023:Glycoproteins; C571326:MOG protein, human; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D011994:Recombinant Proteins; C443660:major capsid protein, Cytomegalovirus; C517781:myelin oligodendrocyte glycoprotein (34-56); C517783:polyoxyethanyl 600 cholesteryl sebacate; D011092:Polyethylene Glycols; D002784:Cholesterol