title: Application of response surface methodology to cell immobilization for the production of palatinose.
abstract: Response surface methodology (RSM), based on multivariate non-linear model, was applied to study the interactions and optimization of the immobilization parameters for cell entrapment, namely alginate concentration, cell loading and bead diameter using Erwinia rhapontici NCPPB 1578 that produced palatinose. ANOVA analysis and statistical parameters calculations showed that RSM could be used effectively to model and improve a complex system like cell immobilization. Palatinose yield was increased by 40%. The maximum yield of 140 mg/ml was achieved in a batch of 1h at alginate concentration of 5% w/v, cell loading of 5 g l(-1) and 2.25 mm bead diameter. Thus, the E. rhapontici NCPPB 1578 immobilization in alginate bead and subsequent palatinose yield was successfully improved by application of RSM technique.
authors.affiliation: Department of Food Engineering and Technology, Institute of Chemical Technology, Nathalal Parikh Marg, Matunga (E), Mumbai 400 019, India.
authors.forename: Piyushkumar
authors.initials: P
authors.lastname: Mundra
authors.forename: Kiran
authors.initials: K
authors.lastname: Desai
authors.forename: S S
authors.initials: SS
authors.lastname: Lele
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004885:Erwinia / Q000378:metabolism*; D007534:Isomaltose / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis
chemical_list: D007534:Isomaltose; C008189:isomaltulose