Induction of hemolin gene expression by bacterial cell wall components in eri-silkworm, Samia cynthia ricini.
A cDNA clone encoding hemolin was isolated from fat body of immunized Samia cynthia ricini larvae based on subtractive suppression hybridization method. The cDNA encodes 413 amino acid residue open reading frame with an 18 residue predicted signal peptide. The expression of the gene was strongly induced in fat body and midgut by an injection of bacterial cells or peptidoglycans, but very weakly by lipopolysaccharide. The mRNA expression in the fat body was detected as early as 3 h post-injection, and reached the peak level at 12 h.
Bao
Yanyuan
Y
Department of Biochemistry and Biotechnology, Tottori University, Tottori 680-8553, Japan.
Yamano
Yoshiaki
Y
Morishima
Isao
I
D000818:Animals; D001419:Bacteria / Q000737:chemistry; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism; D005216:Fat Body / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D007136:Immunoglobulins / Q000096:biosynthesis / Q000235:genetics*; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics*; D016366:Open Reading Frames; D010457:Peptidoglycan / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D007136:Immunoglobulins; D019476:Insect Proteins; D010457:Peptidoglycan; D012333:RNA, Messenger; C066846:hemolin