Validation of a Rickettsia prowazekii-specific quantitative real-time PCR cassette and DNA extraction protocols using experimentally infected lice.
The presence of R. prowazekii in lice using R. prowazekii-specific qPCR cassettes showed the changes of the rickettsial burden in the lice post-infection.
Rozmajzl
Patrick J
PJ
NMRC-IDD/RDD, 503 Robert Grant Ave., Silver Spring, MD 20910-7500, USA. RozmajzlP@nmrc.navy.mil
Houhamdi
Linda
L
Jiang
Ju
J
Raoult
Didier
D
Richards
Allen L
AL
D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004195:Disease Models, Animal; D008003:Phthiraptera / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012283:Rickettsia prowazekii / Q000235:genetics* / Q000302:isolation & purification; D014438:Typhus, Epidemic Louse-Borne / Q000175:diagnosis*
D004269:DNA, Bacterial