Comparison of immune response against Orientia tsutsugamushi, a causative agent of scrub typhus, in 4-week-old and 10-week-old scrub typhus-infected laboratory mice using enzyme-linked immunosorbent assay technique.
Using the indirect enzyme-linked immunosorbent assay (ELISA) technique, we compared the immune response of specific antibodies (IgM and IgG) in serum samples of scrub typhus-infected and non-infected mice against Orientia sutsugamushi (a causative agent of scrub typhus). Two different age groups (4-week-old and 10-week-old) of ICR laboratory mice were infected with O. tsutsugamushi by the animal passage procedure. Serum samples were detected for scrub typhus-specific antibodies using ELISA technique. Results from determining the presence of IgM and IgG in the serum samples obtained from scrub typhus-infected mice showed that the IgG was first detected on day-13 after the initial infection in both the 4-week-old and 10-week-old mice. The IgG titer levels of both groups were not significantly different. Although the presence of IgM in the in serum samples obtained from scrub typhus-infected mice was first detected on day 13 in the 4-week-old mice and on day-12 in the 10-week-old mice, the IgM titer in the 4-week-old mice was slightly lower than in the 10-week-old mice. Clinical observations of the scrub typhus-infected mice showed that the older mice become ill on day 9 whereas the younger mice exhibited the symptoms on day 12. Considering the earlier detection and slightly higher level of specific IgM antibody, it could be interpreted that the older mice may appear to have responded against O. tsutsugamushi faster than the younger mice.
Lerdthusnee
Kriangkrai
K
Department of Entomology, USAMC-AFRIMS, 315/6 Rajvithi Road, Phya Thai, Bangkok 10400, Thailand. kRiangkRAil@afrims.org.
Jenkitkasemwong
Supak
S
Insuan
Sucheera
S
Leepitakrat
Warisa
W
Monkanna
Taweesak
T
Khlaimanee
Nittaya
N
Chareonsongsermkij
Weerayut
W
Leepitakrat
Surachai
S
Chayaphum
Kwanta
K
Jones
James W
JW
D000375:Aging; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice; D008925:Mites / Q000382:microbiology; D012285:Orientia tsutsugamushi / Q000276:immunology*; D012612:Scrub Typhus / Q000097:blood / Q000276:immunology* / Q000635:transmission; D013997:Time Factors
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M