title: Separation, characterization and identification of boar seminal plasma proteins.
abstract: Methods used for the isolation, separation and characterization of boar seminal plasma proteins are discussed, as well as techniques applied to study their binding properties. Attention is paid to interactions of these proteins with different types of saccharides and glycoconjugates, with membrane phospholipids, and to interactions between proteins. Boar seminal plasma contains different types of proteins: spermadhesins of the AQN and AWN families; DQH and PSP proteins belong to the most abundant. Some of these proteins are bound to the sperm surface during ejaculation and thus protein-coating layers of sperm are formed. Sperms coated with proteins participate in different types of interactions occurring in the course of the reproduction process, e.g. formation of the oviductal sperm reservoir, sperm capacitation, oocyte recognition and sperm binding to the oocyte.
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo n. 2, 166 37 Prague 6, Czech Republic. vjon@img.cas.cz
authors.forename: V
authors.initials: V
authors.lastname: Jonáková
authors.forename: P
authors.initials: P
authors.lastname: Manásková
authors.forename: M
authors.initials: M
authors.lastname: Tichá
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D011485:Protein Binding; D015203:Reproducibility of Results; D029589:Seminal Plasma Proteins / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism; D013075:Sperm Capacitation; D013552:Swine
chemical_list: D029589:Seminal Plasma Proteins; C425042:spermadhesin