In vitro TNF-alpha and IL-6 production by adherent peripheral blood mononuclear cells obtained from type 1 and type 2 diabetic patients evaluated according to the metabolic control.
Tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) levels were evaluated in lipopolysaccharide (LPS)-stimulated cell cultured monocytes obtained from 24 type 1 and type 2 diabetic patients presenting inadequate (IN) or adequate (AD) metabolic control, and in 21 healthy individuals paired to patients for sex and age. The TNF-alpha levels in stimulated cultures of diabetic patients were similar to healthy individuals, and type 1 diabetic patients showed increased IL-6 supernatant levels. The tendency toward increased TNF-alpha and IL-6 levels was observed with metabolic control of type 1 and type 2 diabetic patients, suggesting that the control of diabetes improves the capacity of activation and maintenance of the proinflammatory immune response.
Foss-Freitas
Maria Cristina
MC
Department of Medicine, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Monte Alegre, 14049-900 Ribeirão Preto (SP), Brazil. mcfoss@fmrp.usp.br
Foss
Norma Tiraboschi
NT
Donadi
Eduardo Antonio
EA
Foss
Milton Cesar
MC
D001786:Blood Glucose / Q000032:analysis; D016022:Case-Control Studies; D002448:Cell Adhesion; D002478:Cells, Cultured; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000235:genetics / Q000276:immunology*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics / Q000276:immunology*; D005215:Fasting; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D019410:Interleukin-16 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D001786:Blood Glucose; D006442:Glycated Hemoglobin; D019410:Interleukin-16; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha