Dynamin 2 associates with complexins and is found in the acrosomal region of mammalian sperm.
Previous data showed that complexin I, a SNARE regulatory protein, is localized in and/or around the acrosome and is necessary for the acrosome reaction in sperm. To understand how complexin I regulates the acrosome reaction, we used complexin-GST pulldown assays to identify interacting proteins. We showed that both complexins I and II bound mouse sperm dynamin 2. Dynamin 2 is a 100 kDa GTPase essential to many aspects of endocytosis but its potential role in exocytosis is unknown. Dynamin 2 is expressed in rat testis and widely expressed in other tissues; however, the function of dynamin 2 in germ cells is uncertain. Dynamin 2 protein was detected in mouse testis and was most abundant in or around the developing acrosome of spermatids. In addition, dynamin 2 was co-localized with complexin I in the acrosomal region of mammalian sperm. Its co-localization and interaction with complexin I suggest that dynamin 2 may play a role during acrosome formation and/or acrosomal exocytosis.
Zhao
Longmei
L
Department of Animal Sciences, University of Illinois, Urbana, Illinois 61801, USA.
Shi
Xudong
X
Li
Lingjun
L
Miller
David J
DJ
D000177:Acrosome / Q000378:metabolism*; D020101:Acrosome Reaction / Q000502:physiology; D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D034285:Dynamin II / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011485:Protein Binding; D013079:Sperm Maturation / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine; D013737:Testis / Q000378:metabolism
D033942:Adaptor Proteins, Vesicular Transport; D009419:Nerve Tissue Proteins; C095992:complexin I; C095993:complexin II; D034285:Dynamin II