title: [Evaluation of estrogenic activity for nonylphenol using quantitative real-time RT-PCR method].
abstract: It has been demonstrated that nonylphenol (NP) exerts estrogenic activity. Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to study the VTG-I , VTG-II , CHG-H and CHG-L genes expression in the liver of juvenile medaka exposed to NP at 1, 10, 50, 100 microg/I. for 60 days. The results show that the VTG-I , VTG-II, CHG-H and CHG-L genes expression in the liver of juvenile medaka are induced even at 1 microg/L, significantly. It should be noted that the lowest-observed-effect concentration (LOECs) based on the hepatic vitellogenin (VTG) induction is about 1 microg/L, suggesting that quantitative real-time RT-PCR can detect the estrogenic activity of NP at relatively low concentration, and there is a potential application in evaluating the estrogenic activity of NP in aquatic environment.
authors.affiliation: College of Environmental Science, Peking University, Beijing 100871, China. sailinlin2009@163.com
authors.forename: Lin-lin
authors.initials: LL
authors.lastname: Sai
authors.forename: Zhao-bin
authors.initials: ZB
authors.lastname: Zhang
authors.forename: Jian-ying
authors.initials: JY
authors.lastname: Hu
authors.forename: Yan-feng
authors.initials: YF
authors.lastname: Hou
authors.forename: Dao-jian
authors.initials: DJ
authors.lastname: Sai
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009990:Oryzias / Q000235:genetics*; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014819:Vitellogenins / Q000235:genetics*
chemical_list: D010636:Phenols; D020033:Protein Isoforms; D012333:RNA, Messenger; D014819:Vitellogenins; C025256:nonylphenol