title: Quantitative analysis of ochratoxin A in wine and beer using solid phase extraction and high performance liquid chromatography-fluorescence detection.
abstract: A reliable and accurate method is described for the quantitative analysis of ochratoxin A (OTA) in wine and beer. The method involves the use of disposable non-polar polymeric and aminopropyl solid-phase extraction cartridges to isolate the mycotoxin from alcoholic beverages. Extracts were subsequently analysed using reverse-phase high-performance liquid chromatography-fluorescence detection with post column ammoniation to improve the limit of detection. The precision of the method determined at three levels in both wine and beer was less than 5% (RSD). Standard addition studies in both wine and beer showed that the recovery of OTA varied between 90 and 106% over a concentration range of 0.016-1.284 microg l-1. The detection and quantification limits were shown to be better than 0.004 (S/N = 3) and 0.016 microg l-1 (S/N = 10) for both beer and wine.
authors.affiliation: A Joint Venture Between CSIRO and the Victorian Government, Food Science Australia, PO Box 52, North Ryde, NSW 1670, Australia. varelis@iit.com
authors.forename: Peter
authors.initials: P
authors.lastname: Varelis
authors.forename: Su-Lin L
authors.initials: SL
authors.lastname: Leong
authors.forename: Ailsa
authors.initials: A
authors.lastname: Hocking
authors.forename: Georgina
authors.initials: G
authors.lastname: Giannikopoulos
mesh_terms: D001515:Beer / Q000032:analysis*; D002273:Carcinogens / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis; D009793:Ochratoxins / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods; D014920:Wine / Q000032:analysis*
chemical_list: D002273:Carcinogens; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A