Quantification of free and total sialic acid excretion by LC-MS/MS.
BACKGROUND
The main purpose for measuring urinary free sialic acid (FSA) is to diagnose sialic acid (SA) storage diseases. Elevated amounts of conjugated sialic acid (CSA) are observed in several diseases indicating the need to quantify CSA as well. A LC-MS/MS method for quantification of FSA and total sialic acid (TSA) in urine is developed and validated.


METHODS
FSA is analyzed directly after filtration of urine samples. For determination of TSA an enzymatic (neuraminidase) and a chemical (acid) hydrolysis were compared. 13C3-sialic acid was used as internal standard. LC-MS/MS was performed in negative electrospray ionisation mode with multiple reaction monitoring of transitions m/z 308.2-->87.0 (SA) and m/z 311.2-->90.0 (13C3-SA). CSA was calculated by subtracting FSA from TSA.


RESULTS
Limit of detection for FSA and TSA was 0.3 and 1.7 micromol/L, respectively. Limit of quantification for FSA and TSA was 1.0 and 5.0 micromol/L. Intra- and inter-assay variations of FSA were 4.6% and 6.6% (n=10) for FSA and 6.5% and 3.6% (n=10) for TSA. Linearity was tested till 7800 micromol/L (r2=0.9998). Values of SA analyzed after neuraminidase- or acid hydrolysis treatment were comparable. Urine samples from patients with inborn errors of SA (related) metabolism were analyzed and compared with age-related reference values.


CONCLUSIONS
A method has been developed for routine determination of urinary FSA and TSA. The method is rapid, specific, robust and sensitive. Age-related reference values for FSA, TSA and CSA were determined and improved diagnostic efficacy.
van der Ham
Maria
M
Department of Metabolic and Endocrine Diseases, University Medical Centre Utrecht, The Netherlands.
Prinsen
Berthil H C M T
BH
Huijmans
Jan G M
JG
Abeling
Nicolaas G G M
NG
Dorland
Bert
B
Berger
Ruud
R
de Koning
Tom J
TJ
de Sain-van der Velden
Monique G M
MG
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002853:Chromatography, Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D019158:N-Acetylneuraminic Acid / Q000032:analysis / Q000652:urine*; D015203:Reproducibility of Results; D029461:Sialic Acid Storage Disease / Q000652:urine; D053719:Tandem Mass Spectrometry / Q000379:methods*
D019158:N-Acetylneuraminic Acid