[Relationship between the malignant mesothelioma and simian virus 40 in China: a study of 17 cases].
OBJECTIVE
To investigate whether simian virus 40 (SV40) was related to patients of malignant mesothelioma in China.


METHODS
Paraffin-embeded samples of 17 patients with malignant mesothelioma were collected. After isolation of DNA from paraffin blocks, polymerase chain reaction (PCR) analyses were performed using three different sets of primer for detection of SV40 large T antigen gene. These samples were also immunohistochemically evaluated for expression of SV40 TAg protein with two different anti-SV40 Tag (Pab101 and Ab-2).


RESULTS
Only one of the three primer pairs successfully amplified SV40 genome in three malignant mesothelioma samples. No immunopositive staining for SV40 TAg was found in any of the samples.


CONCLUSIONS
The study shows that malignant mesothelioma in China may be independent of SV40 infection.
Jin
Mu-lan
ML
Department of Pathology, Beijing Chao Yang Hospital of Capital Medical University, Beijing 100020, China. kinmokuran@163.com
Li
Xue
X
Luo
Jing
J
Zhao
Hong-ying
HY
Liu
Yang
Y
D000328:Adult; D000368:Aged; D000957:Antigens, Viral, Tumor / Q000235:genetics / Q000378:metabolism; D002681:China; D005260:Female; D054884:Host-Pathogen Interactions; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008654:Mesothelioma / Q000473:pathology* / Q000821:virology; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000473:pathology* / Q000821:virology; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D014412:Tumor Virus Infections / Q000473:pathology* / Q000821:virology; D055815:Young Adult
D000957:Antigens, Viral, Tumor