title: Analysis of ligand binding curves on basis of mean intrinsic thermodynamic quantities.
abstract: The mean intrinsic thermodynamic quantity can be defined by considering the relative population of complex species in the solution and the value of intrinsic thermodynamic quantity corresponds to each step of ligation. In the present study a new method is introduced for analysis of experimental ligand binding data on basis of mean intrinsic thermodynamic quantities. In this regard, a deviation parameter was defined by comparing the non-interacting system with the cooperative interactive one. This parameter can be calculated just by estimation of the first binding constant. A set of relations between this deviation parameter and other binding characteristics, such as mean intrinsic Gibbs free energy of binding and mean Gibbs free energy of site-site interaction, have been developed. This model presents binding mechanism in a unified way that is simple, yet stringent, more straightforward, more reliable and informative. This analyzing method has been successfully applied for evaluation of various systems such as oxygen binding to hemoglobin, laurate and warfarin binding to human serum albumin, and reveals some new biological features of these binding systems.
authors.affiliation: Laboratory of Biophysical Chemistry, Department of Chemistry, Isfahan University, Isfahan 81746-73441, Iran. bordbar@chem.ui.ac.ir
authors.forename: Abdol-Khalegh
authors.initials: AK
authors.lastname: Bordbar
authors.forename: Hamid
authors.initials: H
authors.lastname: Dazhampanah
authors.forename: Seyed Habib-Allah
authors.initials: SH
authors.lastname: Mousavi
authors.forename: Thomas
authors.initials: T
authors.lastname: Haertle
mesh_terms: D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007848:Laurates / Q000378:metabolism; D008024:Ligands; D008956:Models, Chemical; D010100:Oxygen / Q000378:metabolism; D010313:Partial Pressure; D012709:Serum Albumin / Q000378:metabolism; D013816:Thermodynamics; D014859:Warfarin / Q000378:metabolism
chemical_list: D006454:Hemoglobins; D007848:Laurates; D008024:Ligands; D012709:Serum Albumin; D014859:Warfarin; D010100:Oxygen