A new species of Epichloë symbiotic with Chinese grasses.
Epichloë species are fungal symbionts (endophytes) of grasses, six European and four North American biological species in genus Epichloë have been described in previous researches. In this study we describe a new Epichloë species, Epichloë yangzii Li et Wang, found in natural symbioses with Roegneria kamoji native to China. We investigated the host specificity, morphology, interfertility tests and molecular phylogenetic evidences of this new species. The results indicated that E. yangzii is host specific and seedborne. Most morphological characteristics of this new species are typical in the genus. However differences are evident in several features including size of perithecia, asci and ascospores. In mating tests E. yangzii was not interfertile with E. elymi isolates from related hosts in genera Elymus. Phylogenetic relationships based on sequences of beta-tubulin gene (tub2) introns and translation elongation factor 1-alpha gene (tef1) introns showed that members of the new species grouped into exclusive clades with high bootstrap value.
Li
Wei
W
Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Ji
Yan-ling
YL
Yu
Han-shou
HS
Wang
Zhi-wei
ZW
D031702:Agropyron / Q000033:anatomy & histology / Q000382:microbiology* / Q000502:physiology; D002967:Claviceps / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D039642:Eukaryotic Initiation Factors / Q000235:genetics; D016681:Genome, Fungal; D010802:Phylogeny; D013559:Symbiosis; D014404:Tubulin / Q000235:genetics
D039642:Eukaryotic Initiation Factors; D014404:Tubulin