title: Recovery of drugs of abuse from the Immunalysis Quantisal oral fluid collection device.
abstract: Drug recovery from a new oral fluid collection device was assessed. The evaluation was performed in vitro at three physiologically relevant concentrations for the following substances: amphetamine, methamphetamine, morphine, codeine, cocaine, benzoylecgonine, methadone, oxazepam, and Delta(9)-tetrahydrocannabinol (THC). Drug-free and drug-fortified controls were prepared and their concentration verified by liquid chromatography-tandem mass spectrometry. Aliquots of the controls were then "collected" with the device (n=3) using the manufacturer's recommended procedure. Collected samples were stored for 12 h to simulate shipping before analysis. Fresh, non-"collected" aliquots of each pool (n=3) were concurrently analyzed. The drug recoveries from the Quantisal were expressed as a mean percentage of the concurrently analyzed aliquots that were not subjected to device collection. Recoveries for oxazepam exceeded 97%, for amphetamine and methamphetamine exceeded 93%, and for opioids all exceeded 91%. The recoveries of cocaine were >91% and >82% for its polar metabolite, benzoylecgonine. Especially noteworthy was the recovery of THC from the Quantisal collector (81.3-91.4%). When compared with recoveries reported from other collection devices, the Quantisal was clearly superior.
authors.affiliation: Center for Human Toxicology, University of Utah, Salt Lake City, Utah 84108, USA.
authors.forename: Oscar
authors.initials: O
authors.lastname: Quintela
authors.forename: Dennis J
authors.initials: DJ
authors.lastname: Crouch
authors.forename: David M
authors.initials: DM
authors.lastname: Andrenyak
mesh_terms: D002851:Chromatography, High Pressure Liquid; D053593:Forensic Toxicology / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis* / Q000276:immunology; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D013287:Illicit Drugs