title: The t(14;19)(q32;q13)-positive small B-cell leukaemia: a clinicopathologic and cytogenetic study of seven cases.
abstract: The t(14;19)(q32;q13), involving the BCL3 locus at chromosome 19q13 and the immunoglobulin heavy chain gene at 14q32, is a rare recurrent cytogenetic abnormality identified in B-cell neoplasms, most of which have been classified as chronic lymphocytic leukaemia (CLL) in the literature. We describe the clinicopathological, immunophenotypic and cytogenetic findings in seven patients with B-cell neoplasms associated with t(14;19)(q32;q13). There were five men and two women, with a median age of 48 years (range 33-68). All had absolute lymphocytosis, six had lymphadenopathy, and one had splenomegaly. Lymphocytes in blood and bone marrow aspirate smears were predominantly small and cytologically atypical. Flow cytometric immunophenotyping showed an atypical immunophenotype with low CLL scores. The growth pattern in bone marrow biopsy specimens was interstitial to diffuse; immunohistochemical stains were positive for bcl3 and negative for cyclin D1. Lymph node biopsy specimens of two patients revealed total architectural effacement by neoplasm with proliferation centres. In addition to t(14;19), cytogenetic studies demonstrated trisomy 12 in five patients. These results suggest that B-cell neoplasms with the t(14;19)(q32;q13) present frequently as leukaemia composed of small B-lymphocytes and share many features with CLL. However, these neoplasms also differ from CLL cytologically and in their immunophenotype.
authors.affiliation: Department of Hematopathology, MD Anderson Cancer Centre, The University of Texas, Houston, TX 77030, USA. yhuh@mdanderson.org
authors.forename: Yang O
authors.initials: YO
authors.lastname: Huh
authors.forename: Lynne V
authors.initials: LV
authors.lastname: Abruzzo
authors.forename: George Z
authors.initials: GZ
authors.lastname: Rassidakis
authors.forename: Nilima
authors.initials: N
authors.lastname: Parry-Jones
authors.forename: Ellen
authors.initials: E
authors.lastname: Schlette
authors.forename: Vasantha
authors.initials: V
authors.lastname: Brito-Bapabulle
authors.forename: Estella
authors.initials: E
authors.lastname: Matutes
authors.forename: Andrew
authors.initials: A
authors.lastname: Wotherspoon
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Keating
authors.forename: L Jeffrey
authors.initials: LJ
authors.lastname: Medeiros
authors.forename: Daniel
authors.initials: D
authors.lastname: Catovsky
mesh_terms: D000328:Adult; D000081242:B-Cell Lymphoma 3 Protein; D002871:Chromosome Banding; D002883:Chromosomes, Human, Pair 14; D002888:Chromosomes, Human, Pair 19; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015448:Leukemia, B-Cell / Q000145:classification / Q000235:genetics*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000145:classification; D008297:Male; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014178:Translocation, Genetic
chemical_list: D000081242:B-Cell Lymphoma 3 Protein; C000631344:BCL3 protein, human; D007143:Immunoglobulin Heavy Chains; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors