Expression of hepatitis B surface antigen in Saccharomyces cerevisiae utilizing glyceraldeyhyde-3-phosphate dehydrogenase promoter of Pichia pastoris.
An expression vector constructed from genes of Pichia pastoris was applied for heterologous gene expression in Saccharomyces cerevisiae. Recombinant hepatitis B surface antigen was synthesized by cloning hepatitis B virus 'S' gene under the control of glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter of Pichia pastoris in Saccharomyces cerevisiae. Hepatitis B surface antigen was constitutively expressed, was stable and exhibited approximately 20-22 nm particle formation. Stability and absence of toxicity to the host with the expression vector indicates the expression system can be applied for large-scale production.
Vellanki
Ravi Nagaraj
RN
Centre for Biotechnology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, India.
Komaravelli
Narayana
N
Tatineni
Radhika
R
Mangamoori
Lakshmi Narasu
LN
D015139:Blotting, Southern; D015153:Blotting, Western; D015966:Gene Expression Regulation, Fungal; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008957:Models, Genetic; D010843:Pichia / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D006514:Hepatitis B Surface Antigens; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases