[Development of Dasypyrum genome specific marker by using wheat microsatellites].
One hundred and two SSR primer pairs, distributed in chromosome 1A to 7A, 1B to 7B, 1D to 7D of Triticum aestivum, were investigated on Dasypyrum breviaristatum, D.villosum, wheat-Dasypyrum amphiploids and its derivatives, with the control of common wheat Chinese Spring and elite wheat cultivars. A specific polymorphic DNA fragment of about 400 bp (415 bp-long by sequenced, named Xgwm301/415) amplified by primer pair Xgwm301 was obtained in all lines containing Dasypyrum chromosomes, but there were not the case in the tested common wheat. Furthermore, PCR analysis was performed on a set of T. aestivum-Dasypyrum addition, the result showed that all the seven pairs of villosum chromosomes contain Xgwm301/415. Therefore, Xgwm301/415 is a genome-specific polymorphic DNA segment for genera of Dasypyrum, and it could be used as a molecular marker for detection of chromosomes of Dasypyrum in wheat.
Liu
Cheng
C
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. lch6688407@163.com
Yang
Zu-June
ZJ
Feng
Juan
J
Zhou
Jian-Ping
JP
Chi
Shi-Hua
SH
Ren
Zheng-Long
ZL
D001483:Base Sequence; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013696:Temperature; D014908:Triticum / Q000166:cytology / Q000235:genetics*
D018744:DNA, Plant; D005819:Genetic Markers