title: Molecular anatomy of a trafficking organelle.
abstract: Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.
authors.affiliation: Department of Neurobiology, Max-Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Takamori
authors.forename: Matthew
authors.initials: M
authors.lastname: Holt
authors.forename: Katinka
authors.initials: K
authors.lastname: Stenius
authors.forename: Edward A
authors.initials: EA
authors.lastname: Lemke
authors.forename: Mads
authors.initials: M
authors.lastname: Grønborg
authors.forename: Dietmar
authors.initials: D
authors.lastname: Riedel
authors.forename: Henning
authors.initials: H
authors.lastname: Urlaub
authors.forename: Stephan
authors.initials: S
authors.lastname: Schenck
authors.forename: Britta
authors.initials: B
authors.lastname: Brügger
authors.forename: Philippe
authors.initials: P
authors.lastname: Ringler
authors.forename: Shirley A
authors.initials: SA
authors.lastname: Müller
authors.forename: Burkhard
authors.initials: B
authors.lastname: Rammner
authors.forename: Frauke
authors.initials: F
authors.lastname: Gräter
authors.forename: Jochen S
authors.initials: JS
authors.lastname: Hub
authors.forename: Bert L
authors.initials: BL
authors.lastname: De Groot
authors.forename: Gottfried
authors.initials: G
authors.lastname: Mieskes
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Moriyama
authors.forename: Jürgen
authors.initials: J
authors.lastname: Klingauf
authors.forename: Helmut
authors.initials: H
authors.lastname: Grubmüller
authors.forename: John
authors.initials: J
authors.lastname: Heuser
authors.forename: Felix
authors.initials: F
authors.lastname: Wieland
authors.forename: Reinhard
authors.initials: R
authors.lastname: Jahn
mesh_terms: D000818:Animals; D008055:Lipids / Q000737:chemistry; D008958:Models, Molecular; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry; D051381:Rats; D013050:Spectrometry, Fluorescence; D013572:Synaptic Vesicles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D008055:Lipids; D011506:Proteins