Enhanced bronchial expression of extracellular matrix proteins in chronic obstructive pulmonary disease.
Remodeling of airways and blood vessels is an important feature in chronic obstructive pulmonary disease (COPD). By using immunohistochemical analysis, we examined bronchial expression patterns of various extracellular matrix (ECM) components such as collagens (subtypes I, III, and IV), fibronectin, and laminin beta2 in patients with COPD (forced expiratory volume in 1 second [FEV1] <or=75%; n = 15) and without COPD (FEV1 >or=85%; n = 16) and correlated expression data with lung function. Quantitative analysis revealed enhanced levels (P < .01) of total collagens I, III, and IV in surface epithelial basement membrane (SEBM) and collagens I and III in bronchial lamina propria (P < .02) and adventitia (P < .05) in COPD. Distinct and increased (P < .05) vascular expression of fibronectin accounts for intimal vascular fibrosis, whereas laminin beta2 (P < .05) was elevated in airway smooth muscle (ASM). FEV1 values inversely correlated with collagens in the SEBM, fibronectin in bronchial vessels, and laminin in the ASM. Our data suggest that COPD exhibits increased bronchial deposition of ECM proteins that contribute to deteriorated lung function and airway remodeling.
Kranenburg
Andor R
AR
Department of Pharmacology, Erasmus MC, University Medical Centre, Rotterdam, the Netherlands.
Willems-Widyastuti
Anna
A
Moori
Wolter J
WJ
Sterk
Peter J
PJ
Alagappan
Vijay K T
VK
de Boer
Willem I
WI
Sharma
Hari S
HS
D000328:Adult; D000368:Aged; D001980:Bronchi / Q000737:chemistry / Q000473:pathology* / Q000503:physiopathology; D024042:Collagen Type I / Q000032:analysis; D024061:Collagen Type III / Q000032:analysis; D024141:Collagen Type IV / Q000032:analysis; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D005260:Female; D005353:Fibronectins / Q000032:analysis; D005541:Forced Expiratory Volume; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology
D024042:Collagen Type I; D024061:Collagen Type III; D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; C058783:laminin beta2