title: The effect of Na+-K+ ATPase inhibition by ouabain on histamine release from human cutaneous mast cells.
abstract: BACKGROUND
  There are controversial reports on the effect of sodium-potassium adenosine triphosphatase (Na+-K+ ATPase) inhibition on mast cell mediator release. Some of them have indicated that ouabain (strophanthin G), a specific Na+-K+ ATPase inhibitor, inhibited the release, whereas the others have shown that ouabain had no effect or even had a stimulatory effect on the mediator secretion. Most of these studies have utilized animal-derived mast cells. The aim of this study was to determine the effect of Na+-K+ ATPase inhibition on human skin mast cells.
  
  
  METHODS
  Unpurified and purified mast cells were obtained from newborn foreskins and stimulated by calcium ionophore A23187 (1 microM) for 30 min following a 1 hr incubation with various concentrations (10(-4) to 10(-8) M) of ouabain. Histamine release was assayed by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  The results indicated that ouabain had no significant effect on the non-immunologic histamine release from human skin mast cells, in vitro.
  
  
  CONCLUSIONS
  Na+-K+ ATPase inhibition by ouabain had no significant effect on the non-immunologic histamine release from human cutaneous mast cells and suggested differences between human and animal mast cells.
authors.affiliation: Department of Dermatology, Inonu University School of Medicine, 44069, Malatya, Turkey. mstfsenol@hotmail.com
authors.forename: Mustafa
authors.initials: M
authors.lastname: Senol
authors.forename: I Halil
authors.initials: IH
authors.lastname: Ozerol
authors.forename: Asha V
authors.initials: AV
authors.lastname: Patel
authors.forename: David P
authors.initials: DP
authors.lastname: Skoner
mesh_terms: D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D052816:Foreskin / Q000166:cytology; D006632:Histamine / Q000032:analysis; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D010042:Ouabain; D006632:Histamine; D000254:Sodium-Potassium-Exchanging ATPase