Simple multichannel system for the measurement of the net water flux across biological tissues.
This paper describes the development of a simple system for measurement of net water movement through biological membrane barriers. The system is based on the detection of a water meniscus inside a polyethylene tube, which reflects the water movement inside one hemichamber of a modified Ussing chamber containing a membrane barrier. The detection device consists of a commercial computer-controlled flat bed scanner and specifically developed software. This system allows one to perform a relatively high number of individual experiments per physical unit. It is a flexible and affordable device, which allows comparatively more information per unit to be obtained than previously described methods.
Fernández-Miyakawa
Mariano E
ME
California Animal Health and Food Safety Laboratory, San Bernardino Branch, University of California-Davis, 105 W Central Avenue, San Bernardino, CA 92408, USA. mmiyakaw@cahfs.ucdavis.edu
Dorr
Ricardo
R
Fernández
Luis E
LE
Uzal
Francisco A
FA
Ibarra
Cristina
C
D001834:Body Water / Q000378:metabolism*; D003016:Clostridium perfringens; D003205:Computing Methodologies; D004847:Epithelial Cells; D006801:Humans; D008566:Membranes / Q000502:physiology*; D012984:Software