The protective efficacy of chlamydial protease-like activity factor vaccination is dependent upon CD4+ T cells.
We have previously determined the protective efficacy of intranasal vaccination with chlamydial protease-like activity factor (CPAF) against genital chlamydial infection. Since T-helper 1 (Th1) responses are important for anti-chlamydial immunity, we examined the contribution of CD4(+) T cells in CPAF mediated immunity against intravaginal (i.vag.) Chlamydia muridarum infection in C57BL/6 mice. CPAF+IL-12 vaccination induced antigen-specific CD4(+) T cells that secreted elevated levels of IFN-gamma, and generated strong humoral responses. The protective effects of CPAF vaccination against genital chlamydial challenge were abrogated by anti-CD4 neutralizing antibody treatment. Moreover, anti-chlamydial immunity could be adoptively transferred to naïve recipients using CPAF-specific CD4(+) T cells. Therefore, CPAF mediated anti-chlamydial immunity is highly dependent upon antigen-specific CD4(+) T cells.
Murphey
Cathi
C
Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USA.
Murthy
Ashlesh K
AK
Meier
Patricia A
PA
Neal Guentzel
M
M
Zhong
Guangming
G
Arulanandam
Bernard P
BP
D000281:Administration, Intranasal; D019264:Adoptive Transfer; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D049109:Cell Proliferation; D002689:Chlamydia / Q000201:enzymology* / Q000276:immunology*; D010450:Endopeptidases / Q000276:immunology*; D005260:Female; D018664:Interleukin-12 / Q000008:administration & dosage / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010057:Oviducts / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology
D001428:Bacterial Vaccines; D018664:Interleukin-12; D010450:Endopeptidases; C425923:CPA factor