title: Novel sites of aldose reductase immunolocalization in normal and streptozotocin-diabetic rats.
abstract: Glucose metabolism by aldose reductase (AR) is implicated in the pathogenesis of many diabetic complications, including neuropathy. We have re-evaluated the distribution of AR in the sciatic nerve and dorsal root ganglion (DRG) of normal rats, expanded these observations to describe the location of AR in the spinal cord and footpad skin, and investigated whether diabetes alters the distribution of AR. In sciatic nerve, AR was restricted to cytoplasm of myelinated Schwann cells and endothelial cells of epineurial, but not endoneurial, blood vessels. AR immunoreactivity (IR) was present in satellite cells in the DRG. In skin, AR-IR was detected in vascular endothelial cells, Schwann cells of myelinated fibers, and axons of perivascular sympathetic nerves. AR was localized selectively to oligodendrocytes of the white matter of spinal cord. The distribution of AR-IR in sciatic nerve, DRG, skin, and spinal cord was not altered by up to 12 weeks of streptozotocin-induced diabetes. Identification of perineuronal satellite cells, oligodendrocytes, and perivascular sympathetic nerves as AR-expressing cells reveals them as cellular sites with the potential to contribute to diabetic neuropathy.
authors.affiliation: Department of Pathology (Neuropathology), School of Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Jiang
authors.forename: Nigel A
authors.initials: NA
authors.lastname: Calcutt
authors.forename: Khara M
authors.initials: KM
authors.lastname: Ramos
authors.forename: Khara M
authors.initials: KM
authors.lastname: Rames
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Mizisin
mesh_terms: D000449:Aldehyde Reductase / Q000378:metabolism*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D042783:Endothelial Cells / Q000201:enzymology; D005260:Female; D005727:Ganglia, Spinal / Q000201:enzymology*; D007150:Immunohistochemistry; D009836:Oligodendroglia / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D027161:Satellite Cells, Perineuronal / Q000201:enzymology; D012583:Schwann Cells / Q000201:enzymology; D012584:Sciatic Nerve / Q000201:enzymology*; D012867:Skin / Q000201:enzymology* / Q000294:innervation; D013116:Spinal Cord / Q000201:enzymology*; D013311:Streptozocin
chemical_list: D013311:Streptozocin; D000449:Aldehyde Reductase