title: Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6).
abstract: Cancer/testis (CT) antigens are the protein products of germ line-associated genes that are activated in a wide variety of tumors and can elicit autologous cellular and humoral immune responses. CT antigens can be divided between those that are encoded on the X chromosome (CT-X antigens) and those that are not (non-X CT antigens). Among the CT-X antigens, the melanoma antigen gene (MAGE) family, defined by a shared MAGE homology domain (MHD), is the largest. CT-X genes are frequently expressed in a coordinate manner in cancer cells, and their expression appears to be modulated by epigenetic mechanisms. The expression of CT-X genes is associated with advanced disease and poor outcome in different tumor types. We used the yeast two-hybrid system to identify putative MHD-interacting proteins. The MHD of MAGE-C1 (CT7) was used as bait to screen a human testis cDNA library. This study identified NY-ESO-1 (CT6) as a MAGE-C1 binding partner. Immunoprecipitation and immunofluorescence staining confirmed MAGE-C1 interaction with NY-ESO-1, and cytoplasmic co-localization of both proteins in melanoma cells. Co-expression of these two genes was found to occur in cancer cell lines from different origins, as well as in primary tumors (multiple myeloma and non-small cell lung cancer samples). This is the first report of direct interaction between two CT antigens and may be pertinent in the light of the frequently coordinated expression of these proteins.
authors.affiliation: Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA.
authors.forename: Hearn J
authors.initials: HJ
authors.lastname: Cho
authors.forename: Otavia L
authors.initials: OL
authors.lastname: Caballero
authors.forename: Sacha
authors.initials: S
authors.lastname: Gnjatic
authors.forename: Valéria C C
authors.initials: VC
authors.lastname: Andrade
authors.forename: Gisele W
authors.initials: GW
authors.lastname: Colleoni
authors.forename: Andre L
authors.initials: AL
authors.lastname: Vettore
authors.forename: Hasina H
authors.initials: HH
authors.lastname: Outtz
authors.forename: Sheila
authors.initials: S
authors.lastname: Fortunato
authors.forename: Nasser
authors.initials: N
authors.lastname: Altorki
authors.forename: Cathy A
authors.initials: CA
authors.lastname: Ferrera
authors.forename: Ramon
authors.initials: R
authors.lastname: Chua
authors.forename: Achim A
authors.initials: AA
authors.lastname: Jungbluth
authors.forename: Yao-Tseng
authors.initials: YT
authors.lastname: Chen
authors.forename: Lloyd J
authors.initials: LJ
authors.lastname: Old
authors.forename: Andrew J G
authors.initials: AJ
authors.lastname: Simpson
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D005786:Gene Expression Regulation; D015723:Gene Library; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D011485:Protein Binding; D013737:Testis / Q000737:chemistry; D014407:Tumor Cells, Cultured; D020798:Two-Hybrid System Techniques
chemical_list: D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; C110905:MAGEC1 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins