Amelioration of graft ischemia-reperfusion injury by breviscapine in rat small bowel transplantation.
OBJECTIVE
We sought to evaluate the effects of breviscapine to ameliorate graft ischemia-reperfusion (I/R) injury in a rat small bowel transplantation model.


METHODS
Thirty-six recipients were randomly divided into three groups (n = 12): operative controls, in which grafts were implanted immediately after harvesting; an I/R control group with grafts preserved in cold lactated Ringer's solution at 4 degrees C for 4 hours before transplantation; and a breviscapine group wherein the graft was treated in the same way as the I/R control group but breviscapine (25 mg/kg/d) was injected intraperitoneally into both the donors and the recipients for 3 days before the operation of and into the recipients after transplantation. We compared the pathological scores for I/R injury, apoptosis index, and content of malondialdehyde (MDA) in the graft.


RESULTS
Breviscapine diminished the pathological change caused by I/R injury (breviscapine vs I/R control on 24 hours after operation, 1.50 +/- 0.55 vs 2.17 +/- 0.75; P < .05), decreased the apoptotic index (breviscapine vs I/R control at 24 hours after operation, 27.33 +/- 0.167 vs 73.83 +/- 0.077; P < .05), and reduced the graft tissue content of MDA (breviscapine vs I/R control on 24 hours after operation, 1.717 +/- 0.131 vs 3.167 +/- 0.196; P < .05).


CONCLUSIONS
Breviscapine may protect the transplanted small intestine against I/R injury during transplantation in rats.
Ma
R
R
Department of General Surgery, Qilu Hospital of Shandong University, Shandong, China.
Sun
J-L
JL
Zhang
X-Q
XQ
Dai
Y
Y
Sun
J-Z
JZ
D000818:Animals; D004365:Drugs, Chinese Herbal; D005419:Flavonoids / Q000627:therapeutic use*; D007421:Intestine, Small / Q000637:transplantation*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D014184:Transplantation, Homologous / Q000473:pathology*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C061097:breviscapine