Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus).
A stable GFP-expressing (GFP(+)LJES1) cell strain was developed from the LJES1 cells obtained from sea perch (Lateolabrax japonicus,) embryos. GFP(+)LJES1 cells were induced in vitro by RA to differentiate into a variety of cell types and also had the ability to form embryoid body-like structures in suspension culture. To determine the differentiation potential of LJES1 cells in vivo, GFP(+)LJES1 cells were transplanted into sea perch and zebrafish embryos at mid-blastula stage. Twenty out of 478 transplanted sea perch embryos contained GFP-expressing LJES1 cells 24 h after microinjection. Fifteen chimera embryos developed into fry. In these chimeras, the GFP(+)LJES1 cells contributed to a variety of tissues including the head and trunk. In zebrafish, 221 embryos were microinjected with GFP(+)LJES1 cells and 22 chimera embryos and fries expressing GFP were obtained. Donor GFP(+)LJES1 cells contributed to various tissues in head and trunk of zebrafish embryos and hatched fry.
Chen
Song-Lin
SL
Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Qingdao 266071, China. chensl@ysfri.ac.cn
Sha
Zhen-Xia
ZX
Ye
Han-Qing
HQ
Liu
Yang
Y
Tian
Yong-Sheng
YS
Hong
Yunhan
Y
Tang
Qi-Sheng
QS
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D002678:Chimera / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008856:Microscopy, Fluorescence; D010471:Perches / Q000196:embryology* / Q000502:physiology*; D014162:Transfection / Q000662:veterinary; D015027:Zebrafish / Q000196:embryology
D049452:Green Fluorescent Proteins