Homologs of the sexually induced gene 1 (sig1) product constitute the stramenopile mastigonemes.
The tripartite tubular mastigoneme on the anterior flagellum is a morphological feature that characterizes the stramenopiles. Mastigonemes are significant and potentially informative structures not only from the viewpoint of systematics, but also of cell biology. Nevertheless, few biochemical studies have been reported on stramenopile mastigonemes. The flagella of Scytosiphon lomentaria (Phaeophyceae) were successfully isolated and analyzed using SDS-PAGE followed by protein sequencing. The partial amino acid sequence of one flagellar protein (115kDa) showed high similarity with the sexually induced gene 1 (sig1) product of centric diatoms. A polyclonal antibody against the 115-kDa protein reacted not only to the shaft of mastigonemes in Scytosiphon lomentaria, but also another distinctly different stramenopile flagellate, Sulcochrysis biplastida (Dictyochophyceae). Therefore, we propose that the 115-kDa protein (i.e. Sig1 homologs) is a constituent of the tubular shaft of the mastigoneme.
Honda
Daiske
D
Department of Biology, Faculty of Science and Engineering, Konan University, Okamoto, Higashinada, Kobe 658-8501, Japan. dhonda@konan-u.ac.jp
Shono
Takanori
T
Kimura
Kei
K
Fujita
Satoshi
S
Iseki
Mineo
M
Makino
Yumiko
Y
Murakami
Akio
A
D000595:Amino Acid Sequence; D000818:Animals; D048028:DNA, Algal / Q000032:analysis; D056890:Eukaryota / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D005407:Flagella / Q000648:ultrastructure*; D008969:Molecular Sequence Data; D000459:Phaeophyceae / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D048028:DNA, Algal; D011506:Proteins