Inositol phosphoglycan P-type in preeclampsia: a novel marker?
A state of insulin resistance has been demonstrated in active preeclampsia, and women with clinical evidence of insulin resistance are at higher risk to develop this syndrome during pregnancy. Recently, inositol phosphoglycan P-type, a putative second messenger of insulin action, has been implicated in the pathophysiology of preeclampsia and is increased in the placenta, amniotic fluid, and maternal urine of preeclamptic women compared with normal pregnant women. We report here a case-control study to assess the potential of urinary levels of inositol phosphoglycan P-type as a screening test for preeclampsia. Twenty-seven preeclamptic women and 47 healthy pregnant women were recruited. A polyclonal antibody-based ELISA was developed to detect levels of inositol phosphoglycan P-type in urine. Its content in urinary specimens was found to be 30-fold higher in preeclamptic subjects than control subjects (329.1+/-21.8 versus 9.2+/-1.5; P<0.001), with a higher level in all of the preeclamptic cases. For 6 women who developed preeclampsia, >1 gestational date sample of urine was available, and retrospective analysis showed a significant time-related increase of the urinary level of inositol phosphoglycan P-type <or=7 weeks before clinical diagnosis of preeclampsia. Urinary level of inositol phosphoglycan P-type increased after diagnosis indicating a possible pathophysiological threshold level and steeply decreased after delivery.
Williams
Philip J
PJ
Department of Molecular Pathology, Molecular Medicine Unit, Royal Free and University College Medical School, London, United Kingdom.
Gumaa
Khalid
K
Scioscia
Marco
M
Redman
Christopher W
CW
Rademacher
Thomas W
TW
D000328:Adult; D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007295:Inositol Phosphates / Q000652:urine*; D011134:Polysaccharides / Q000652:urine*; D011225:Pre-Eclampsia / Q000209:etiology / Q000652:urine*; D011247:Pregnancy; D012307:Risk Factors
D015415:Biomarkers; D007295:Inositol Phosphates; D011134:Polysaccharides; C028039:inositol phosphate glycan