Calcium modulates interactions between bacteria and hydroxyapatite.
Bacterial adhesion onto hydroxyapatite is known to depend on the surface properties of both the biomaterial and the bacterial strain, but less is known about the influence of the composition of the aqueous medium. Here, the adhesion of Streptococcus mutans and 3 different Lactobacilli on powdered hydroxyapatite was shown to change with Ca2+ concentration. The effect depends on the surface properties of each strain. Adhesion of Lactobacillus fermentum and salivarius (and of Streptococcus mutans at low Ca2+) was enhanced with increasing Ca2+ concentration. Lactobacillus casei was efficiently removed by adhesion on hydroxyapatite, even without Ca2+ addition, and the effect of this ion was only marginal. The results are interpreted in terms of Ca2+-mediated adhesion, and relative to the hydrophobic properties of each strain and the electrical properties of the bacterial and solid surfaces (electrophoretic mobility).
Venegas
S C
SC
Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, 4000, San Miguel de Tucumán, Tucumán, Argentina.
Palacios
J M
JM
Apella
M C
MC
Morando
P J
PJ
Blesa
M A
MA
D000327:Adsorption; D001422:Bacterial Adhesion / Q000187:drug effects*; D001672:Biocompatible Materials / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry*; D004586:Electrophoresis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007778:Lactobacillus / Q000145:classification / Q000187:drug effects*; D007780:Lacticaseibacillus casei / Q000187:drug effects; D052197:Limosilactobacillus fermentum / Q000187:drug effects; D011208:Powders; D012463:Saliva / Q000502:physiology; D013295:Streptococcus mutans / Q000187:drug effects*; D013499:Surface Properties
D001672:Biocompatible Materials; D011208:Powders; D017886:Durapatite; D002118:Calcium