Use of sequence-based typing for investigation of a case of nosocomial legionellosis.
A fatal case of nosocomial legionellosis in a low prevalence region (Calgary, Alberta, Canada) prompted investigation into the source of infection. Hospital water systems contaminated with Legionella pneumophila have been shown to pose a risk to compromised patients. Typing of an L. pneumophila serogroup 1 strain isolated from the patient using sequence-based typing (SBT) and amplified fragment length polymorphism (AFLP) analysis linked it to a persistent and widespread strain isolated from the hospital water system establishing a nosocomial mode of acquisition. Different SBT and AFLP patterns were determined for non-epidemiologically linked cases and isolates from different hospitals.
Wong
Sallene
S
Provincial Laboratory for Public Health (Microbiology), Calgary site, Calgary, Alberta, Canada.
Pabbaraju
Kanti
K
Provincial Laboratory for Public Health (Microbiology), Calgary site, Calgary, Alberta, Canada.
Burk
Veronica F
VF
Provincial Laboratory for Public Health (Microbiology), Calgary site, Calgary, Alberta, Canada.
Broukhanski
George C
GC
Ontario Ministry of Health and Long-Term Care, Toronto, Ontario, Canada.
Fox
Julie
J
Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.|Provincial Laboratory for Public Health (Microbiology), Calgary site, Calgary, Alberta, Canada.
Louie
Tom
T
Infection Prevention and Control, Calgary Health Region, Calgary, Alberta, Canada.
Mah
Manuel W
MW
Infection Prevention and Control, Calgary Health Region, Calgary, Alberta, Canada.
Bernard
Kathryn
K
National Microbiology Laboratory, Winnipeg, Manitoba, Canada.
Tilley
Peter A G
PAG
Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.|Provincial Laboratory for Public Health (Microbiology), Calgary site, Calgary, Alberta, Canada.
D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D002170:Canada / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000209:etiology*; D004269:DNA, Bacterial / Q000235:genetics; D017809:Fatal Outcome; D005260:Female; D006801:Humans; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007876:Legionellosis / Q000453:epidemiology / Q000209:etiology*; D008666:Metalloendopeptidases / Q000235:genetics; D008969:Molecular Sequence Data; D018410:Pneumonia, Bacterial / Q000453:epidemiology / Q000209:etiology*; D012150:Polymorphism, Restriction Fragment Length; D018272:Porins / Q000235:genetics; D012307:Risk Factors; D020539:Sequence Analysis, Protein; D013045:Species Specificity; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D018272:Porins; C072453:major outer membrane protein, Legionella pneumophila; D008666:Metalloendopeptidases