title: Naturally occurring anti-Escherichia coli protein antibodies in the sera of healthy humans cause analytical interference in a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay for serodiagnosis of severe acute respiratory syndrome.
abstract: We reported the analytical interference of anti-Escherichia coli protein (EP) antibodies in human sera and residual EP in a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay as a possible source of false positives in severe acute respiratory syndrome serodiagnosis. The rate of false positives was significantly reduced by adding mouse anti-EP antiserum in the blocking step.
authors.affiliation: Department of Zoology, Kadoorie Biological Science Building, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, China.
authors.forename: Chi Wai
authors.initials: CW
authors.lastname: Yip
authors.forename: Chung Chau
authors.initials: CC
authors.lastname: Hon
authors.forename: Fanya
authors.initials: F
authors.lastname: Zeng
authors.forename: Ken Y C
authors.initials: KY
authors.lastname: Chow
authors.forename: Kwok Hung
authors.initials: KH
authors.lastname: Chan
authors.forename: Joseph S M
authors.initials: JS
authors.lastname: Peiris
authors.forename: Frederick C C
authors.initials: FC
authors.lastname: Leung
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D000086462:Coronavirus Nucleocapsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D029968:Escherichia coli Proteins / Q000276:immunology*; D006801:Humans; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology / Q000302:isolation & purification*; D012698:Serologic Tests / Q000379:methods; D045169:Severe Acute Respiratory Syndrome / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000086462:Coronavirus Nucleocapsid Proteins; D029968:Escherichia coli Proteins; D019590:Nucleocapsid Proteins