[Evaluation of estrogenic activity for nonylphenol using quantitative real-time RT-PCR method].
It has been demonstrated that nonylphenol (NP) exerts estrogenic activity. Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to study the VTG-I , VTG-II , CHG-H and CHG-L genes expression in the liver of juvenile medaka exposed to NP at 1, 10, 50, 100 microg/I. for 60 days. The results show that the VTG-I , VTG-II, CHG-H and CHG-L genes expression in the liver of juvenile medaka are induced even at 1 microg/L, significantly. It should be noted that the lowest-observed-effect concentration (LOECs) based on the hepatic vitellogenin (VTG) induction is about 1 microg/L, suggesting that quantitative real-time RT-PCR can detect the estrogenic activity of NP at relatively low concentration, and there is a potential application in evaluating the estrogenic activity of NP in aquatic environment.
Sai
Lin-lin
LL
College of Environmental Science, Peking University, Beijing 100871, China. sailinlin2009@163.com
Zhang
Zhao-bin
ZB
Hu
Jian-ying
JY
Hou
Yan-feng
YF
Sai
Dao-jian
DJ
D000818:Animals; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009990:Oryzias / Q000235:genetics*; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014819:Vitellogenins / Q000235:genetics*
D010636:Phenols; D020033:Protein Isoforms; D012333:RNA, Messenger; D014819:Vitellogenins; C025256:nonylphenol