Discovering potential pathogens among fungi identified as nonsporulating molds.
Fungal infections are increasing, particularly among immunocompromised hosts, and a rapid diagnosis is essential to initiate antifungal therapy. Often fungi cannot be identified by conventional methods and are classified as nonsporulating molds (NSM). We sequenced internal transcribed spacer regions from 50 cultures of NSM and found 16 potential pathogens that can be associated with clinical disease. In selected clinical settings, identification of NSM could prove valuable and have an immediate impact on patient management.
Pounder
June I
JI
Associated Regional and University Pathologists, Inc., Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA. june.pounder@aruplab.com
Simmon
Keith E
KE
Barton
Claudia A
CA
Hohmann
Sheri L
SL
Brandt
Mary E
ME
Petti
Cathy A
CA
D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D016867:Immunocompromised Host; D016533:Mycological Typing Techniques / Q000379:methods*; D009181:Mycoses / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer