title: Evaluation of broad-spectrum anthelmintic activity in a novel assay against Haemonchus contortus, Trichostrongylus colubriformis and T. sigmodontis in the gerbil Meriones unguiculatus.
abstract: The gerbil Meriones unguiculatus, infected with three species of nematodes, each located in a separate part of the gastrointestinal tract, provided a reliable laboratory assay for the evaluation of broad-spectrum anthelmintic activity. Gerbils harbouring 6-day-old infections of Haemonchus contortus, Trichostrongylus colubriformis and T. sigmodontis were given selected broad-spectrum anthelmintics by gavage. Three benzimidazoles, thiabendazole, oxfendazole and albendazole, a tetrahydropyrimidine, morantel, an imidazothiazole, levamisole hydrochloride, a macrocyclic lactone, ivermectin and an experimental natural product, paraherquamide, were active against all three nematodes at various dosages. Trichostrongylus colubriformis was most sensitive to levamisole hydrochloride, morantel, thiabendazole and paraherquamide whereas ivermectin, oxfendazole and albendazole were more effective against H. contortus. All compounds were active against the caecal nematode T. sigmodontis although it was less sensitive than T. colubriformis. Haemonchus contortus was more sensitive than T. sigmodontis to all anthelmintics tested except thiabendazole.
authors.affiliation: stanton94@earthlink.net
authors.forename: D A
authors.initials: DA
authors.lastname: Ostlind
authors.forename: S
authors.initials: S
authors.lastname: Cifelli
authors.forename: W G
authors.initials: WG
authors.lastname: Mickle
authors.forename: S K
authors.initials: SK
authors.lastname: Smith
authors.forename: D V
authors.initials: DV
authors.lastname: Ewanciw
authors.forename: B
authors.initials: B
authors.lastname: Rafalko
authors.forename: T
authors.initials: T
authors.lastname: Felcetto
authors.forename: A
authors.initials: A
authors.lastname: Misura
mesh_terms: D015766:Albendazole / Q000627:therapeutic use; D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use*; D001562:Benzimidazoles / Q000627:therapeutic use; D005260:Female; D005849:Gerbillinae / Q000469:parasitology*; D006188:Haemonchiasis / Q000188:drug therapy; D006189:Haemonchus / Q000187:drug effects; D007212:Indolizines / Q000627:therapeutic use; D007559:Ivermectin; D007978:Levamisole / Q000627:therapeutic use; D008297:Male; D009015:Morantel / Q000627:therapeutic use; D021261:Parasitic Sensitivity Tests; D013141:Spiro Compounds / Q000627:therapeutic use; D013827:Thiabendazole / Q000627:therapeutic use; D014251:Trichostrongyloidea / Q000187:drug effects*; D014252:Trichostrongyloidiasis / Q000188:drug therapy* / Q000662:veterinary; D014254:Trichostrongylus / Q000187:drug effects
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; D007212:Indolizines; D013141:Spiro Compounds; D007978:Levamisole; D007559:Ivermectin; D009015:Morantel; D015766:Albendazole; D013827:Thiabendazole; C011030:oxfendazole; C063985:paraherquamide