Comparison of sensitivity and specificity in three commercial foot-and-mouth disease virus non-structural protein ELISA kits with swine sera in Taiwan.
Three commercialized ELISA kits for the detection of antibodies to the non-structural proteins (NSPs) of FMD virus were compared, using sera from uninfected, vaccinated, challenged and naturally infected pigs. The kinetics of the antibody response to NSPs was compared on sequential serum samples in swine from challenge studies and outbreaks. The results showed that ELISA A (UBI) and ELISA B (CEDI) had better sensitivity than that of the 3ABC recombinant protein-based ELISA C (Chekit). The peak for detection of antibodies to NSPs in ELISA C was significantly delayed in sera from natural infection and challenged swine as compared to the ELISA A and B. The sensitivity of the three ELISAs gradually declined during the 6-month post-infection as antibodies to NSP decline. ELISA kits A and B detected NSP antibody in 50% of challenged pigs by the 9-10th-day and 7-8th-day post-challenge, respectively. ELISA B and C had better specificity than ELISA A on sequential serum samples obtained from swine immunized with a type O FMD vaccine commercially available in Taiwan. Antibody to NSPs before vaccination was not detected in swine not exposed to FMD virus, however, antibody to NSPs was found in sera of some pigs after vaccination. All assays had significantly lower specificity when testing sera from repeatedly vaccinated sows and finishers in 1997 that were tested after the 1997 FMD outbreak. However, when testing sera from repeatedly vaccinated sows or finishers in 2003-2004, the specificity for ELISAs A, B and C were significantly better than those in 1997. This effect was less marked for ELISA A. The ELISA B was the best test in terms of the highest sensitivity and specificity and the lowest reactivity with residual NSP in vaccinates.
Chen
S P
SP
Division of Animal Medicine, Animal Technology Institute Taiwan, Chunan, Miaoli, Taiwan. spchen@mail.atit.org.tw
Ellis
T M
TM
Lee
M C
MC
Cheng
I C
IC
Yang
P C
PC
Lin
Y L
YL
Jong
M H
MH
Robertson
I D
ID
Edwards
J R
JR
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary*; D005536:Foot-and-Mouth Disease / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D013624:Taiwan; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D014765:Viral Vaccines
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines