A proteomic analysis of the effect of MAPK pathway activation on L-glutamate-induced neuronal cell death.
Oxidative stress has been implicated in the pathogenesis of neuronal degenerative diseases. It is also widely known that oxidative stress induces mitogen-activated protein kinase (MAPK) signaling cascades. In this study, we used proteomic analysis to investigate the role of the MAPK pathway in oxidative stress-induced neuronal cell death. The results demonstrated that several proteins, including eukaryotic translation elongation factor 2 (eEF2) and enolase I, showed a differential expression pattern during the neuronal cell death process, and this was MAPK pathway dependent. Several chaperone and cytoskeletal proteins including heat shock protein 70, calreticulin, vimentin, prolyl 4-hydroxylase beta polypeptide, and transgelin 2 were up-or down-regulated, despite their expressions not depending on the MAPK pathway. These findings strongly suggest that the expressions of proteins which play protective roles are independent of the MAPK pathway. On the other hand, eEF2 and enolase I may be the downstream targets of the MAPK pathway.
Kang
Sunghyun
S
Korean Research Institute of Bioscience and Biotechnology, 52 Eoeun-Dong, Yusung-Gu, Daejeon, Republic of Korea.
Kim
Eun Young
EY
Bahn
Young Jae
YJ
Chung
Jin Woong
JW
Lee
Do Hee
DH
Park
Sung Goo
SG
Yoon
Tae-Sung
TS
Park
Byoung Chul
BC
Bae
Kwang-Hee
KH
D002070:Butadienes / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D004789:Enzyme Activation / Q000187:drug effects; D020869:Gene Expression Profiling; D018698:Glutamic Acid / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects*; D009570:Nitriles / Q000494:pharmacology; D040901:Proteomics
D002070:Butadienes; D009570:Nitriles; C113580:U 0126; D018698:Glutamic Acid; D020928:Mitogen-Activated Protein Kinases