[Type IV collagenase expression in human prostate carcinoma cell lines with different metastasis potentials].
OBJECTIVE
To investigate the correlation between type IV collagenase expression and metastatic potential of human prostate carcinoma cells.


METHODS
Human prostate carcinoma cell lines (LNCaP and C4-2B) with different metastatic potentials were selected. Using SABC immunocytochemical staining and zymographic analysis, we detected the difference of type IV collagenase expression and activity between the two cell lines.


RESULTS
The expression of type IV collagenase (MMP-2, MMP-9) could be detected in both LNCaP and C4-2B, mainly in cytoplasm, much higher in C4-2B than in LNCaP (P < 0.01). The activities of type IV collagenase in the conditioned medium of prostatic carcinoma cell LNCaP was the lowest, and the quantitative estimation values of MMP-2 and MMP-9 were 0.89 +/- 0.02 and 0.86 +/- 0.01, respectively. However, the quantitative estimation values of MMP-2 and MMP-9 in the conditioned medium of C4-2B were 96.32 +/- 4.36 and 33.89 +/- 1.84, respectively. Compared with LNCaP, the activity of type IV collagenase in C4-2B was much higher (P < 0.01).


CONCLUSIONS
Compared with LNCaP, the higher production capability of type IV collagenase in C4-2B may possibly contribute to its invasive and metastatic potentials. The expression of type IV collagenase of human prostate carcinoma cells is closely correlated to the metastatic and invasive potential.
Zhang
Lin-Lin
LL
Department of Urology, First Affiliated Hospital of Xi'an Jiaotong University Medical School, Xi'an, Shaanxi 710061, China. zhangll1026@stu.xjtu.edu.cn
He
Da-Lin
DL
Li
Xiang
X
Li
Lei
L
Zhu
Guo-Dong
GD
Wang
Xin-Yang
XY
D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000473:pathology
D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9