title: [Pathology of AIDS-related lymphadenopathy and its relationship to expression of CD4 + CD25 + regulatory T cells in lymphoid node tissue].
abstract: OBJECTIVE
  To observe the pathology of AIDS-related lymphadenopathy and its relationship to the expression and distribution of CD4 + CD25 + regulatory T cells in lymphoid node tissue.
  
  
  METHODS
  Totally 22 biopsy and 13 autopsy lymphoid node tissues from HIV-positive patients were examined under microscopy and pathological staging was performed. Specific marker for CD4 + CD25 + regulatory T cells in lymphoid node tissue was detected with anti-Foxp3 monoclonal antibody by immunohistochemistry.
  
  
  RESULTS
  Among all the 35 specimens, 5, 4, 14, and 12 specimens were histopathologically staged from 1 to 4, respectively. FoxP3 were detected in all lymphoid node tissues. The distribution of FoxP3-positive lymphocytes were mainly in intermediate zone of follicle and cortical area in stages 1 and 2. The counts of FoxP3-positive lymphocytes remarkably decreased in stages 3 and 4, following depletion of lymphocytes.
  
  
  CONCLUSIONS
  CD4 + CD25 + regulatory T cells exist in lymphoid node tissue of patients with HIV infection. Their amounts decrease or deplete along with the progression of AIDS-related lymphadenopathy.
authors.affiliation: Department of Pathology, Beijing Ditan Hospital, Beijing 100011, China. langzw@21cn.com
authors.forename: Zhen-wei
authors.initials: ZW
authors.lastname: Lang
authors.forename: Pei-qing
authors.initials: PQ
authors.lastname: Ma
authors.forename: Hong-xin
authors.initials: HX
authors.lastname: Zhao
authors.forename: Meng-Dong
authors.initials: MD
authors.lastname: Lan
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Dao
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Shen
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Shi
authors.forename: Zhi-chun
authors.initials: ZC
authors.lastname: Ma
authors.forename: Pei-zhi
authors.initials: PZ
authors.lastname: Wang
authors.forename: Fu-jie
authors.initials: FJ
authors.lastname: Zhang
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000473:pathology; D000328:Adult; D018791:CD4 Lymphocyte Count; D005260:Female; D051858:Forkhead Transcription Factors / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000276:immunology* / Q000473:pathology; D008206:Lymphatic Diseases / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors