title: Magnetic silica extraction for low-viremia human immunodeficiency virus type 1 genotyping.
abstract: Nucleic acid extraction and human immunodeficiency virus type 1 (HIV-1) genotyping using the NucliSens miniMAG platform and the TruGene HIV-1 genotyping kit gave HIV-1 sequence data from HIV-1-negative plasma spiked with 100 copies/ml reference HIV-1 RNA and from low-viremia clinical samples (<500 copies/ml) without the need for ultracentrifugation or nested second-round PCR.
authors.affiliation: Clinical Virology Department, GlaxoSmithKline, P.O. Box 13398, Research Triangle Park, NC 27709-3398, USA. daniel.r.mcclernon@gsk.com
authors.forename: D R
authors.initials: DR
authors.lastname: McClernon
authors.forename: E
authors.initials: E
authors.lastname: Ramsey
authors.forename: M St
authors.initials: MS
authors.lastname: Clair
mesh_terms: D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008280:Magnetics; D012367:RNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012822:Silicon Dioxide; D019562:Viral Load; D014766:Viremia / Q000821:virology*
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic; D012822:Silicon Dioxide