title: Bongkrekic acid and atractyloside inhibits chloride channels from mitochondrial membranes of rat heart.
abstract: The aim of this work was to characterize the effect of bongkrekic acid (BKA), atractyloside (ATR) and carboxyatractyloside (CAT) on single channel properties of chloride channels from mitochondria. Mitochondrial membranes isolated from a rat heart muscle were incorporated into a bilayer lipid membrane (BLM) and single chloride channel currents were measured in 250/50 mM KCl cis/trans solutions. BKA (1-100 microM), ATR and CAT (5-100 microM) inhibited the chloride channels in dose-dependent manner. The inhibitory effect of the BKA, ATR and CAT was pronounced from the trans side of a BLM and it increased with time and at negative voltages (trans-cis). These compounds did not influence the single channel amplitude, but decreased open dwell time of channels. The inhibitory effect of BKA, ATR and CAT on the mitochondrial chloride channel may help to explain some of their cellular and/or subcellular effects.
authors.affiliation: Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34 Bratislava, Slovak Republic.
authors.forename: Lubica
authors.initials: L
authors.lastname: Malekova
authors.forename: Viera
authors.initials: V
authors.lastname: Kominkova
authors.forename: Miroslav
authors.initials: M
authors.lastname: Ferko
authors.forename: Peter
authors.initials: P
authors.lastname: Stefanik
authors.forename: Olga
authors.initials: O
authors.lastname: Krizanova
authors.forename: Attila
authors.initials: A
authors.lastname: Ziegelhöffer
authors.forename: Adam
authors.initials: A
authors.lastname: Szewczyk
authors.forename: Karol
authors.initials: K
authors.lastname: Ondrias
mesh_terms: D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D015153:Blotting, Western; D001865:Bongkrekic Acid / Q000378:metabolism* / Q000494:pharmacology; D018118:Chloride Channels / Q000037:antagonists & inhibitors*; D004305:Dose-Response Relationship, Drug; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D051336:Mitochondrial Membranes / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats
chemical_list: D018118:Chloride Channels; D001865:Bongkrekic Acid; D001278:Atractyloside; C003853:carboxyatractyloside