Spectrophotometric determination of fluoxetine by batch and flow injection methods.
A rapid, simple, and accurate spectrophotometric method is presented for the determination of fluoxetine by batch and flow injection analysis methods. The method is based on fluoxetine competitive complexation reaction with phenolphthalein-beta-cyclodextrin (PHP-beta-CD) inclusion complex. The increase in the absorbance of the solution at 554 nm by the addition of fluoxetine was measured. The formation constant for fluoxetin-beta-CD was calculated by non-linear least squares fitting. Fluoxetine can be determined in the range 7.0 x 10(-6)-2.4 x 10(-4) mol l(-1) and 5.0 x 10(-5)-1.0 x 10(-2) mol l(-1) by batch and flow methods, respectively. The limit of detection and limit of quantification were respectively 4.13 x 10(-6) mol l(-1) and 1.38 x 10(-5) mol l(-1) for batch and 2.46 x 10(-5) mol l(-1) and 8.22 x 10(-5) mol l(-1) for flow method. The sampling rate in flow injection analysis method was 80+/-5 samples h(-1). The method was applied to the determination of fluoxetine in pharmaceutical formulations and after addition to human urine samples.
Afkhami
Abbas
A
Faculty of Chemistry, Bu-Ali-Sina University, Hamadan 65174, Iran. afkhami@basu.ac.ir
Madrakian
Tayyebeh
T
Khalafi
Lida
L
D000928:Antidepressive Agents / Q000737:chemistry*; D005473:Fluoxetine / Q000737:chemistry*; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*
D000928:Antidepressive Agents; D004364:Pharmaceutical Preparations; D005473:Fluoxetine