title: Self-splicing of a group IIC intron: 5' exon recognition and alternative 5' splicing events implicate the stem-loop motif of a transcriptional terminator.
abstract: Bacterial IIC introns are a newly recognized subclass of group II introns whose ribozyme properties have not been characterized in detail. IIC introns are typically located downstream of transcriptional terminator motifs (inverted repeat followed by T's) or other inverted repeats in bacterial genomes. Here we have characterized the self-splicing activity of a IIC intron, B.h.I1, from Bacillus halodurans. B.h.I1 self-splices in vitro through hydrolysis to produce linear intron, but interestingly, additional unexpected products were formed that were highly dependent on ionic conditions. These products were determined to represent alternative splicing events at the 5' junction and cleavages throughout the RNA transcript. The alternative splicing and cleavage events occurred at cryptic splice sites containing stem-loop and IBS1 motifs, suggesting that the 5' exon is recognized by both elements. These results provide the first example of a group II intron that uses 5' splice sites nonadjacent to the ribozyme structure. Furthermore, the data suggest that IIC introns differ from IIA and IIB introns with respect to 5' exon definition, and that the terminator stem-loop substitutes in part for the missing IBS2-EBS2 (intron and exon binding sites 2) interaction.
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Drive. NW, Calgary, Alberta T2N 1N4, Canada.
authors.forename: Navtej
authors.initials: N
authors.lastname: Toor
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Robart
authors.forename: Joshua
authors.initials: J
authors.lastname: Christianson
authors.forename: Steven
authors.initials: S
authors.lastname: Zimmerly
mesh_terms: D017398:Alternative Splicing; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D005091:Exons; D014644:Genetic Variation; D007438:Introns; D009154:Mutation; D009690:Nucleic Acid Conformation; D022821:RNA Splice Sites; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry*; D013728:Terminator Regions, Genetic
chemical_list: D022821:RNA Splice Sites; D012329:RNA, Bacterial; D016337:RNA, Catalytic