High production of laccase by a new basidiomycete, Trametes sp.
A new basidiomycete, Trametes sp. 420, produced laccase at 6,810 U l(-1) (268 mg, 25.4 U mg(-1) protein for guaiacol) in glucose medium and 7,870 U l(-1) (310 mg) in cellobiose medium with induction by 0.5 mM Cu(2+) and 6 mM o-toluidine. Laccase isozyme E (LacE) was the sole laccase in the fermentation products. It was stable at pH 5-9 and below 70 degrees C over 30 min. The K (m) values of LacE for four substrates (guaiacol ABTS, 2,6-dimethoxyphenol and syringaldazine) varied from 5 to 245 muM. The activity of LacE was strongly inhibited by NaN(3) but not by EDTA or dimethylsulfoxide. LacE at 0.5 U l(-1) could decolorize industrial dyes. The open reading frame of the lacE gene was 2,130 bp and was interrupted by 10 introns. It displayed a high homology to laccases from other fungi.
Tong
Pingui
P
School of Life Science & Modern Experiment Technology Center, Anhui University, Hefei, Anhui, China. yzxiao@ahu.edu.cn
Hong
Yuzhi
Y
Xiao
Yazhong
Y
Zhang
Min
M
Tu
Xiaoming
X
Cui
Tengjiao
T
D001487:Basidiomycota / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002475:Cellobiose / Q000378:metabolism; D003300:Copper / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D019810:Sodium Azide / Q000494:pharmacology; D013379:Substrate Specificity; D013696:Temperature; D014052:Toluidines / Q000494:pharmacology
D014052:Toluidines; D002475:Cellobiose; D003300:Copper; D019810:Sodium Azide; D004492:Edetic Acid; C023622:2-toluidine; D042845:Laccase; D005947:Glucose