title: Elevated levels of phosphorylated fibrinogen-alpha-isoforms and differential expression of other post-translationally modified proteins in the plasma of ovarian cancer patients.
abstract: We evaluated the differentially expressed proteins in the plasma of ovarian cancer (OVC) patients using 2-D SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with post-translational modification (PTM) specific stains after the removal of six high-abundance proteins. The pooled plasma from patients with stage III or IV OVC was compared to a pooled postmenopausal age-matched control. Several proteins were identified as differentially expressed in the plasma of OVC patients. Among them, the phosphorylated fibrinogen-alpha-chain isoform (containing fibrinopeptide-A) was found to be up-regulated. Previously in our laboratory, phosphorylated fibrinopeptide-A was found to be up-regulated in the low molecular weight fraction of serum derived from OVC patients. We examined the levels of phosphorylated fibrinogen-alpha-chain in each patient that constituted the pooled plasma using Western blot, mass spectrometry (MS), and PTM specific stains. Phosphoprotein bands containing fibrinogen-alpha-chain fragments showed up-regulation in all OVC patients.
authors.affiliation: Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ogata
authors.forename: Carrie J
authors.initials: CJ
authors.lastname: Heppelmann
authors.forename: Carrie J
authors.initials: CJ
authors.lastname: Heppelmann
authors.forename: M Cristine
authors.initials: MC
authors.lastname: Charlesworth
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Madden
authors.forename: Melinda N
authors.initials: MN
authors.lastname: Miller
authors.forename: Kimberly R
authors.initials: KR
authors.lastname: Kalli
authors.forename: William A
authors.initials: WA
authors.lastname: Cliby
authors.forename: H Robert
authors.initials: HR
authors.lastname: Bergen
authors.forename: Diana A
authors.initials: DA
authors.lastname: Saggese
authors.forename: David C
authors.initials: DC
authors.lastname: Muddiman
mesh_terms: D000368:Aged; D001798:Blood Proteins / Q000032:analysis*; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005344:Fibrinopeptide A / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood*
chemical_list: D001798:Blood Proteins; D005344:Fibrinopeptide A