title: Integrated isotachophoretic preconcentration with zone electrophoresis separation on a quartz microchip for UV detection of flavonoids.
abstract: A quartz microchip integrated isotachophoretic (ITP) preconcentration with zone electrophoresis (ZE) separation was fabricated using a novel multi-point pressure method featured in normal temperature and lower pressure during bonding process. ITP followed by subsequential ZE of two flavonoids, quercetin and isorhamnetin on the microchip was performed consecutively on the homemade microfluidic workstation with UV detection, resulting in a decreased detectable concentration of 32-fold, compared to the ZE mode only, and their detection limits decreased down to 0.2 microg/mL and 1.2 microg/mL, respectively.
authors.affiliation: Dalian Institute of Chemical Physics, Graduate School of Chinese Academy of Sciences, Dalian, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.forename: Xiaomian
authors.initials: X
authors.lastname: Zhou
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Huaiqing
authors.initials: H
authors.lastname: Huang
authors.forename: Zhongpeng
authors.initials: Z
authors.lastname: Dai
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qin
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
mesh_terms: D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D011791:Quartz / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation*
chemical_list: D005419:Flavonoids; D011791:Quartz