AMI is associated with polymorphisms in the NOS3 and FGB but not in PAI-1 genes in young adults.
BACKGROUND
We investigated the relationship between NOS3, FGB and PAI-1 polymorphisms and endothelial dysfunction and risk factors for acute myocardial infarction (AMI) in young adults.


METHODS
Endothelial function was measured by response to flow mediated vasodilation (FMV) and induced by nitrate (FMN). Biochemical parameters were measured by standard enzymatic methods and plasma total nitrate was analyzed by the NOA system. NOS3 (T-786C, G894T and intron 4A/B STR), FGB (C-148T and G-455A) and PAI-1 (4G/5G) polymorphisms were determined by PCR-RFLP.


RESULTS
Concentrations of total and LDL cholesterol, apo B, triglycerides, nitrate, PAI-1 and fibrinogen were higher and apo AI, HDL cholesterol and FMV were lower in AMI patients than in controls (p<0.001). PAI-1 (p<0.001) but not nitrate was higher in AMI patients with low response to FMV. NOS3 T-786C and FGB C-148T polymorphisms were associated with AMI (p<0.050). NOS3 T-786C was also related to hypertension (p=0.049). NOS3 intron 4A/B STR was associated with increased concentrations of total cholesterol and apo B. NOS3-786TT/894GT haplotype was associated with increased FMV (p=0.018) than the other haplotypes.


CONCLUSIONS
Our data suggest NOS3 and FGB polymorphisms are associated with AMI. NOS3 is also related to hypertension, endothelial dysfunction and variation on serum cholesterol in young adults with AMI.
Sampaio
Marcelo Ferraz
MF
Instituto Dante Pazzanese de Cardiologia, Sao Paulo, SP, Brazil.
Hirata
Mario Hiroyuki
MH
Hirata
Rosario Dominguez Crespo
RD
Santos
Fabiana Cristina Pereira
FC
Picciotti
Raffaella
R
Luchessi
André Ducati
AD
de Quateli Doi
Sonia
S
Armaganijan
Dikran
D
Batlouni
Michel
M
D000328:Adult; D017023:Coronary Angiography; D005260:Female; D005340:Fibrinogen / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009566:Nitrates / Q000097:blood / Q000627:therapeutic use; D052250:Nitric Oxide Synthase Type III / Q000235:genetics* / Q000378:metabolism; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics / Q000378:metabolism; D011110:Polymorphism, Genetic / Q000235:genetics*; D012039:Regional Blood Flow; D012307:Risk Factors
D009566:Nitrates; D017395:Plasminogen Activator Inhibitor 1; D005340:Fibrinogen; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III