title: Applications of flow cytometry to mycoplasmology.
abstract: Flow cytometry has become a valuable tool in different fields of microbiology, such as clinical microbiology, aquatic and environmental microbiology, food microbiology, and biotechnology. It combines direct and rapid assays to determine numbers, biochemical and physiological characteristics of individual cells, revealing the heterogeneity present in a population. This review focuses on the applications of flow cytometry to the field of mycoplasmology. It tries to give a scope of the important breakthroughs which occurred in this field in the last decades, and in the advantages of introducing flow cytometry in research and routine diagnostic procedures of mycoplasmas.
authors.affiliation: Unidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, 35416 Arucas, Spain. passuncao@becarios.ulpgc.es
authors.forename: Patrícia
authors.initials: P
authors.lastname: Assunção
authors.forename: Ruben S
authors.initials: RS
authors.lastname: Rosales
authors.forename: Nuno T
authors.initials: NT
authors.lastname: Antunes
authors.forename: Christian
authors.initials: C
authors.lastname: de la Fe
authors.forename: José B
authors.initials: JB
authors.lastname: Poveda
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial; D000940:Antigenic Variation; D002460:Cell Line; D003470:Culture Media; D005434:Flow Cytometry; D006801:Humans; D009174:Mycoplasma / Q000276:immunology / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis* / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D003470:Culture Media