title: Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I.
abstract: The interactions between oligonucleotides and inorganic cations have been measured by capillary zone electrophoresis. With increasing concentrations of divalent cations (Ca(2+), Mg(2+), Mn(2+) and Ni(2+)) in the running buffer, the migration behavior was evaluated by calculation of the binding constants. Besides these fundamental studies of binding equilibria, different buffer components, tris(hydroxymethyl)aminomethane and 3-(N-morpholino)propanesulfonic acid, have been investigated and their effects on metal ion binding quantified.
authors.affiliation: Department of Chemistry, University of Basel, Spitalstrasse 51, 4056, Basel, Switzerland.
authors.forename: Alexandra R
authors.initials: AR
authors.lastname: Stettler
authors.forename: Valérie
authors.initials: V
authors.lastname: Chaurin
authors.forename: Edwin C
authors.initials: EC
authors.lastname: Constable
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Housecroft
authors.forename: Maria A
authors.initials: MA
authors.lastname: Schwarz
mesh_terms: D002118:Calcium / Q000737:chemistry; D002413:Cations, Divalent / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008274:Magnesium / Q000737:chemistry; D008345:Manganese / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D009025:Morpholines / Q000737:chemistry; D009532:Nickel / Q000737:chemistry; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D014325:Tromethamine / Q000737:chemistry
chemical_list: D002413:Cations, Divalent; D008670:Metals; D009025:Morpholines; D009696:Nucleic Acids; D009841:Oligonucleotides; D014325:Tromethamine; C008550:morpholinopropane sulfonic acid; D008345:Manganese; D009532:Nickel; D004247:DNA; D008274:Magnesium; D002118:Calcium