title: Interaction between hydrogen sulfide/cystathionine gamma-lyase and carbon monoxide/heme oxygenase pathways in aortic smooth muscle cells.
abstract: OBJECTIVE
  To investigate the interaction between hydrogen sulfide (H2S)/cystathionine gamma-lyase (CSE) and carbon monoxide (CO)/heme oxygenase (HO) pathways in aortic smooth muscle cells (ASMC).
  
  
  METHODS
  The ASMCs were divided into the following groups: (1) the control group; (2) the zinc protoporphyrin (ZnPP) 20 micromol/L group; (3) the propargylglycine (PPG) 2 mmol/L, 4 mmol/L and 10 mmol/L groups; and (4) the sodium hydrosulfide (NaHS) 1 x 10(-5) mol/L, 1 x 10(-4) mol/L and 1 x 10(-3) mol/L groups. Each of the groups was further divided into 6 h, 12 h, 18 h and 24 h subgroups. The CO level, represented by carboxyhemoglobin (HbCO) content was measured using a spectrophotometric method and H2S content was detected by a sensitive electrode method. CSE and HO-1 expressions were detected by Western blotting.
  
  
  RESULTS
  The H2S content in the medium and CSE expression by ASMC were markedly increased by ZnPP compared with the control group. HbCO content in the medium and HO-1 expression by the ASMC started strengthening following 24 h treatment with PPG at 2 mmol/L, but were further strengthened following 18 h and 24 h treatment with PPG at 4 mmol/L compared with the controls (P < 0.01). PPG at 10 mmol/L increased the HbCO level in the medium following 18 h treatment and increased HO-1 expression by the ASMC following 12 h treatment. Moreover, NaHS at 1 x 10(-5) mol/L and 1 x 10(-4) mol/L decreased the HbCO level in the medium and HO-1 expression by the ASMC after 6 h and 12 h treatment, while NaHS at 1 x 10(-3) mol/L decreased them at all time points of the treatments.
  
  
  CONCLUSIONS
  The results suggested that endogenous CO/HO and H2S/CSE pathways inhibited each other in ASMC under physiological conditions.
authors.affiliation: Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.
authors.forename: Hong-fang
authors.initials: HF
authors.lastname: Jin
authors.forename: Jun-bao
authors.initials: JB
authors.lastname: Du
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Li
authors.forename: Yan-fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Yin-fang
authors.initials: YF
authors.lastname: Liang
authors.forename: Chao-shu
authors.initials: CS
authors.lastname: Tang
mesh_terms: D000480:Alkynes / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000378:metabolism; D002248:Carbon Monoxide / Q000378:metabolism*; D002263:Carboxyhemoglobin / Q000378:metabolism; D002478:Cells, Cultured; D003542:Cystathionine gamma-Lyase / Q000378:metabolism*; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D011524:Protoporphyrins / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013440:Sulfides / Q000494:pharmacology
chemical_list: D000480:Alkynes; D011524:Protoporphyrins; D013440:Sulfides; C017803:zinc protoporphyrin; C009055:propargylglycine; D002248:Carbon Monoxide; D002263:Carboxyhemoglobin; D051547:Heme Oxygenase-1; D003542:Cystathionine gamma-Lyase; C025451:sodium bisulfide; D005998:Glycine; D006862:Hydrogen Sulfide