title: Methods for analysis of O-linked modifications on epidermal growth factor-like and thrombospondin type 1 repeats.
abstract: The identification of novel forms of O-linked glycosylation on epidermal growth factor and thrombospondin type 1 repeats, and their emerging functional significance, require the development of new methods for their analysis. This chapter describes detailed methods to analyze both the structure and the site of modification of O-fucose and O-glucose glycans on proteins. These methods use both traditional biochemical methods of carbohydrate composition analysis and electrospray ionization-mass spectrometry of glycopeptides.
authors.affiliation: Department of Biochemistry and Cell Biology, SUNY at Stony Brook, Stony Brook, New York, USA.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Nita-Lazar
authors.forename: Robert S
authors.initials: RS
authors.lastname: Haltiwanger
mesh_terms: D000818:Animals; D001671:Biochemistry / Q000379:methods*; D004815:Epidermal Growth Factor / Q000032:analysis / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D019700:Thrombospondin 1 / Q000032:analysis / Q000737:chemistry*
chemical_list: D019700:Thrombospondin 1; D004815:Epidermal Growth Factor; D010100:Oxygen