title: Short-term lifestyle modification alters circulating biomarkers of endothelial health in sedentary, overweight adults.
abstract: Obesity and inactivity are associated with endothelial dysfunction that may contribute to the development of atherosclerosis. We examined the effects of a short-term lifestyle intervention on circulating biomarkers of endothelial health. Nineteen overweight or obese (mean body mass index (BMI): 28.9 +/- 0.7 kg/m2) men and women underwent 6 weeks of body mass reduction induced by moderate energy restriction (approximately 750 kcal/d; 1 kcal = 4.184 kJ) and aerobic training (approximately 400 kcal/d). Fasting serum samples were collected at baseline and after reduction in body mass (week 6) to assess concentrations of nitrotyrosine (NT), secretory phospholipase A2 (sPLA2), and soluble intracellular adhesion molecule-1 (sICAM-1). Body mass was significantly reduced from 81.3 +/- 2.8 to 77.3 +/- 2.6 kg (p < 0.05). Circulating concentrations of NT and sICAM-1 were significantly reduced with treatment (approximately 25% and approximately 10%, respectively), whereas sPLA2 levels were significantly elevated (approximately 45%). Elevations in sPLA2 were negatively correlated with changes in NT (r = -0.58, p = 0.047); reductions in NT did not correlate significantly with reductions in sICAM-1. It appears that circulating markers of endothelial health are susceptible to short-term exercise interventions with modest reduction in body mass, and such a lifestyle modification may improve endothelial health by reducing protein nitration products and cellular adhesion.
authors.affiliation: Department of Nutritional Sciences, University of Missouri, Columbia, MO 65211 USA.
authors.forename: R Scott
authors.initials: RS
authors.lastname: Rector
authors.forename: James R
authors.initials: JR
authors.lastname: Turk
authors.forename: Grace Y
authors.initials: GY
authors.lastname: Sun
authors.forename: Brianne L
authors.initials: BL
authors.lastname: Guilford
authors.forename: Brian W
authors.initials: BW
authors.lastname: Toedebusch
authors.forename: Matt W
authors.initials: MW
authors.lastname: McClanahan
authors.forename: Tom R
authors.initials: TR
authors.lastname: Thomas
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood*; D015992:Body Mass Index; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000503:physiopathology*; D002149:Energy Intake; D015444:Exercise / Q000502:physiology; D005215:Fasting; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D008019:Life Style; D008297:Male; D009765:Obesity / Q000503:physiopathology*; D010741:Phospholipases A / Q000097:blood; D054467:Phospholipases A2; D014443:Tyrosine / Q000031:analogs & derivatives / Q000097:blood
chemical_list: D015415:Biomarkers; D018799:Intercellular Adhesion Molecule-1; C002744:3-nitrotyrosine; D014443:Tyrosine; D010741:Phospholipases A; D054467:Phospholipases A2