[Studies on aclacinomycin spectral characters and the interaction between aclacinomycin and DNA by spectroscopic method].
The interaction of aclacinomycin(ACR) and DNA was investigated by fluorescence spectrum, and the characteristics of the fluorescence and absorption of aclacinomycin (ACR) were studied. The results indicate that there are two situations: in the case that the concentration ratio of ACR to DNA is small, the ACR is intercalated into the stacked base pairs of DNA; in the other case that the concentration ratio of ACR to DNA is great, the interaction between ACR and DNA is complex. The binding constant of the interaction between ACR and DNA, calculated by the fluorescence titration method, is 2.7 x 10(6) mol x L(-1), and the binding site number is about 0.67 base pairs.
Hu
Min
M
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
Zhang
Zhen-Xi
ZX
Shen
Guo-Li
GL
Liu
Ya-Li
YL
D015250:Aclarubicin / Q000031:analogs & derivatives* / Q000737:chemistry; D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000031:analogs & derivatives / Q000737:chemistry; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence
C011157:aclacinomycins; C052890:ethidium-DNA complex; D015250:Aclarubicin; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium