Immunological localization of syndecan-1 in human endometrium throughout the menstrual cycle.
OBJECTIVE
To examine the expression of syndecan-1 in the endometrium during the menstrual cycle.


METHODS
The expression of syndecan-1 was determined by tissue microarray and immunohistochemistry.


METHODS
Academic clinical and research laboratories.


METHODS
Seventy-one regularly cycling women who underwent endometrial biopsy.


METHODS
Endometrial samples representing five stages of the menstrual cycle were used for the study.


METHODS
Semiquantitative analysis by evaluating the intensity of immunohistochemical reactivity of syndecan-1 by using a modified HSCORE.


RESULTS
Endometrial syndecan-1 was expressed in the luminal and glandular epithelium as well as in the stroma throughout the menstrual cycle in a nonsynchronized fashion. In the luminal epithelium, the expression of syndecan-1 was statistically significantly higher in the mid-secretory phase when compared with the proliferative phase. In the stroma, however, the expression of syndecan-1 was down-regulated after ovulation and remained at a low level through the secretory phases. Differences between the proliferative and mid secretory, as well as between the ovulatory and the early, mid, and late secretory phases, all were statistically significant.


CONCLUSIONS
Syndecan-1 is up-regulated in luminal epithelial cells during the mid-secretory phase and is down-regulated in the stroma during the early to late secretory phases. The differential expression of syndecan-1 coincides with the endometrial remodeling throughout the menstrual cycle.
Lai
Tsung-Hsuan
TH
Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
King
Jeremy A
JA
Shih
Ie-Ming
IeM
Vlahos
Nikos F
NF
Zhao
Yulian
Y
D000328:Adult; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D008597:Menstrual Cycle / Q000378:metabolism*; D053668:Syndecan-1 / Q000378:metabolism*; D014018:Tissue Distribution
C508241:SDC1 protein, human; D053668:Syndecan-1