title: Physico-chemical-mechanical and in vitro biological properties of calcium phosphate cements with doped amorphous calcium phosphates.
abstract: Calcium phosphate cements (CPCs) are successfully used as bone substitutes in dentistry and orthopaedic applications. This study investigated the physico-chemical-mechanical properties of and in vitro biological properties (cell response) of CPCs prepared with amorphous calcium carbonate phosphate (ACCP) doped with magnesium (ACCP-Mg), zinc (ACCp-Zn) or fluoride (ACCP-F) ions. The experimental CPC consisted of alpha-TCP, doped ACCP, and MPCM powders as matrix and biphasic calcium phosphate (BCP) granules. X-ray diffraction analysis showed that the matrix converted to apatite with poor crystallinity (reflecting small crystal size) after setting for 24 h, while BCP remained apparently unchanged. Cements with ACCP-F (F-CPC) had shorter setting times and greater compressive strength compared to cements with ACCP-Mg (Mg-CPC) or ACCP-Zn (Zn-CPC). Scanning electron microscopy (SEM) showed that crystals set on Mg-CPC and Zn-CPC were smaller compared to those on F-CPC. The total porosity of Mg-CPC was greater compared to Zn-CPC or F-CPC. Osteoblast-like cells, MC3T3-E1, remained viable and maintained their ability to express alkaline phosphatase in contact with the CPCs with doped ACCPs.
authors.affiliation: Inserm, U 791, 1 place A. Ricordeau, Nantes, F-44042, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Julien
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Khairoun
authors.forename: Racquel Z
authors.initials: RZ
authors.lastname: LeGeros
authors.forename: Severine
authors.initials: S
authors.lastname: Delplace
authors.forename: Paul
authors.initials: P
authors.lastname: Pilet
authors.forename: Pierre
authors.initials: P
authors.lastname: Weiss
authors.forename: Guy
authors.initials: G
authors.lastname: Daculsi
authors.forename: Jean Michel
authors.initials: JM
authors.lastname: Bouler
authors.forename: Jerome
authors.initials: J
authors.lastname: Guicheux
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001843:Bone Cements / Q000737:chemistry* / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000008:administration & dosage* / Q000737:chemistry*; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006244:Hardness; D008422:Materials Testing; D051379:Mice; D008968:Molecular Conformation; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; C020243:calcium phosphate