Quantitative determination of gland mucous cells-type mucin using a monoclonal antibody, HIK1083: its pathophysiological changes in human gastric juice.
BACKGROUND
Pathological alteration in gastric mucosa is caused by Helicobacter pylori infection and is detectable by histological analysis. In particular, the alteration of gland mucous cells (GMCs)-type mucin, which plays a protective role against H. pylori infection, is critical in the pathogenesis of H. pylori-related gastritis. We established an assay for GMCs-type mucin and quantitatively assessed the pathophysiological changes in its content in human gastric juice samples.


METHODS
The assay method for GMCs-type mucin was based on ELISA using a monoclonal antibody (HIK1083), and was used it to measure GMCs-type mucin in gastric juice obtained from patients with or without H. pylori infection.


RESULTS
All the basic characteristics of the current method were satisfactory to quantify the GMCs-type mucin content in gastric juice. The GMCs-type mucin content, but not total mucin content, was significantly higher in patients with H. pylori infection (n=17; 437+/-476 U, mean+/-SD) than in those without H. pylori infection (n=55; 168+/-322 U, p<0.05).


CONCLUSIONS
The current method is suitable for the quantitative analysis of GMCs-type mucin in gastric juice. The change in GMCs-type mucin content in gastric juice may be possibly implicated in the pathophysiology of the gastric mucosa and in the patient's gastric mucosal lesions.
Kubota
Seiko
S
Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
Yamauchi
Kazuyoshi
K
Kumagai
Toshiko
T
Sugano
Mitsutoshi
M
Kawasaki
Kenji
K
Tozuka
Minoru
M
Akamatsu
Taiji
T
Matsuzawa
Kenji
K
Sugiyama
Atsushi
A
Kurihara
Makoto
M
Katsuyama
Tsutomu
T
Ota
Hiroyoshi
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay; D005750:Gastric Juice / Q000276:immunology / Q000378:metabolism*; D005752:Gastric Mucins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D005753:Gastric Mucosa / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013272:Stomach Diseases / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology*; D013552:Swine; D013696:Temperature
D000911:Antibodies, Monoclonal; D005752:Gastric Mucins