Sensitive and specific method for detecting G protein-coupled receptor mRNAs.
G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.
Hansen
Arne
A
Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institute of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA. arnehansen@nidcr.nih.gov
Chen
Yidong
Y
Inman
Jason M
JM
Phan
Quang N
QN
Qi
Zhi-Qing
ZQ
Xiang
Charlie C
CC
Palkovits
Miklos
M
Cherman
Natasha
N
Kuznetsov
Sergei A
SA
Robey
Pamela G
PG
Mezey
Eva
E
Brownstein
Michael J
MJ
D002460:Cell Line; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D012680:Sensitivity and Specificity
D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled