Formation of Pseudorabies virus glycoprotein E/I complex in baculovirus recombinant system.
Glycoproteins E (gE) and I (gI) of Pseudorabies virus (PRV) form a non-covalently bound complex to which a number of functions have been attributed. The gE/gI complex formation was studied using a series of full-length and truncated forms of gE and gI expressed in baculovirus recombinant system. Both glycoproteins were truncated by stepwise removal of their C-terminal parts and their ability to form the complex was studied by radioimmunoprecipitation. It was found that N-terminal domains of gE and gI containing first 122 and 106 aa, respectively, were sufficient for the complex formation.
Tyborowska
J
J
Department of Molecular Virology, University of Gdansk, Kladki 24, 80-822 Gdansk, Poland. jtybor@biotech.univ.gda.pl
Reszka
N
N
Kochan
G
G
Szewczyk
B
B
D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000378:metabolism*; D015531:Radioimmunoprecipitation Assay; D011995:Recombination, Genetic; D018411:Spodoptera / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
D014759:Viral Envelope Proteins; C089580:glycoprotein E, Suid herpesvirus 1; C042966:pseudorabies virus glycoproteins