A liquid chromatography-mass spectrometry method for the quantitation of aristololactam-I in rat plasma.
A sensitive method with liquid chromatography-electrospray ionization mass spectrometry has been developed and validated for the determination of aristololactam-I in rat plasma after oral administration of aristolochic acid-I using finesteride as the internal standard. Chromatographic separation was achieved on a Lichrospher C(18) column using methanol:0.05% acetic acid in water (71:29, v/v) as a mobile phase delivered at a flow rate of 1 mL/min. The assay was linear for aristololactam-I over the range 0.3-300 ng/mL. The analysis of quality control samples demonstrated precision with coefficient of variation less than 20% (n = 5). Absolute recovery of aristololactam-I was 90.4-97.3%. The LC-MS method for the determination of aristololactam-I is sensitive, specific and can be used to investigate the toxicokinetics of aristololactam-I.
Ling
Zhen
Z
New Drug Screen Centre, China Pharmaceutical University, Nanjing, 210038, People's Republic of China.
Jiang
Zhen-zhou
ZZ
Huang
Xin
X
Zhang
Lu-yong
LY
Xu
Xiao-yue
XY
D000818:Animals; D034341:Aristolochic Acids / Q000097:blood* / Q000633:toxicity; D002138:Calibration; D002853:Chromatography, Liquid; D008297:Male; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
D034341:Aristolochic Acids; C121135:aristolactam I