Phosphorothioate oligonucleotides reduce mitochondrial outer membrane permeability to ADP.
G3139, an antisense Bcl-2 phosphorothioate oligodeoxyribonucleotide, induces apoptosis in melanoma and other cancer cells. This apoptosis happens before and in the absence of the downregulation of Bcl-2 and thus seems to be Bcl-2-independent. Binding of G3139 to mitochondria and its ability to close voltage-dependent anion-selective channel (VDAC) have led to the hypothesis that G3139 acts, in part, by interacting with VDAC channels in the mitochondrial outer membrane (21). In this study, we demonstrate that G3139 is able to reduce the mitochondrial outer membrane permeability to ADP by a factor of 6 or 7 with a K(i) between 0.2 and 0.5 microM. Because VDAC is responsible for this permeability, this result strengthens the aforesaid hypothesis. Other mitochondrial respiration components are not affected by [G3139] up to 1 microM. Higher levels begin to inhibit respiration rates, decrease light scattering and increase uncoupled respiration. These results agree with accumulating evidence that VDAC closure favors cytochrome c release. The speed of this effect (within 10 min) places it early in the apoptotic cascade with cytochrome c release occurring at later times. Other phosphorothioate oligonucleotides are also able to induce VDAC closure, and there is some length dependence. The phosphorothioate linkages are required to induce the reduction of outer membrane permeability. At levels below 1 microM, phosphorothioate oligonucleotides are the first specific tools to restrict mitochondrial outer membrane permeability.
Tan
Wenzhi
W
Department of Biology, University of Maryland, College Park, MD 20742, USA.
Lai
Johnathan C
JC
Miller
Paul
P
Stein
C A
CA
Colombini
Marco
M
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D015640:Ion Channel Gating; D008081:Liposomes / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects* / Q000378:metabolism; D008933:Mitochondrial Swelling / Q000187:drug effects; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010539:Permeability; D010743:Phospholipids / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D013873:Thionucleotides / Q000494:pharmacology*; D050994:Voltage-Dependent Anion Channels / Q000502:physiology
D008081:Liposomes; D016376:Oligonucleotides, Antisense; D010743:Phospholipids; D019253:Proto-Oncogene Proteins c-bcl-2; D013873:Thionucleotides; D050994:Voltage-Dependent Anion Channels; D000244:Adenosine Diphosphate; C408162:oblimersen; D045304:Cytochromes c