Physico-chemical-mechanical and in vitro biological properties of calcium phosphate cements with doped amorphous calcium phosphates.
Calcium phosphate cements (CPCs) are successfully used as bone substitutes in dentistry and orthopaedic applications. This study investigated the physico-chemical-mechanical properties of and in vitro biological properties (cell response) of CPCs prepared with amorphous calcium carbonate phosphate (ACCP) doped with magnesium (ACCP-Mg), zinc (ACCp-Zn) or fluoride (ACCP-F) ions. The experimental CPC consisted of alpha-TCP, doped ACCP, and MPCM powders as matrix and biphasic calcium phosphate (BCP) granules. X-ray diffraction analysis showed that the matrix converted to apatite with poor crystallinity (reflecting small crystal size) after setting for 24 h, while BCP remained apparently unchanged. Cements with ACCP-F (F-CPC) had shorter setting times and greater compressive strength compared to cements with ACCP-Mg (Mg-CPC) or ACCP-Zn (Zn-CPC). Scanning electron microscopy (SEM) showed that crystals set on Mg-CPC and Zn-CPC were smaller compared to those on F-CPC. The total porosity of Mg-CPC was greater compared to Zn-CPC or F-CPC. Osteoblast-like cells, MC3T3-E1, remained viable and maintained their ability to express alkaline phosphatase in contact with the CPCs with doped ACCPs.
Julien
Marion
M
Inserm, U 791, 1 place A. Ricordeau, Nantes, F-44042, France.
Khairoun
Ibrahim
I
LeGeros
Racquel Z
RZ
Delplace
Severine
S
Pilet
Paul
P
Weiss
Pierre
P
Daculsi
Guy
G
Bouler
Jean Michel
JM
Guicheux
Jerome
J
D016475:3T3 Cells; D000818:Animals; D001843:Bone Cements / Q000737:chemistry* / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000008:administration & dosage* / Q000737:chemistry*; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006244:Hardness; D008422:Materials Testing; D051379:Mice; D008968:Molecular Conformation; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties
D001843:Bone Cements; D002130:Calcium Phosphates; C020243:calcium phosphate