Developmental competence of cat oocytes from ovaries stored at various temperature for 24 h.
We conducted two experiments to investigate the effects of storage temperature and period for cat ovaries on the meiotic and developmental competence of oocytes collected from the ovaries. In Experiment 1, ovaries were stored in physiological saline for 24 h at 4 C, 23-25 C, or 38 C (cold, room, and incubator temperature groups, respectively), and then oocytes were collected from the ovaries in each group. Morphologically intact oocytes were then selected and cultured in maturation medium for 24 h. Significantly more oocytes reached metaphase II (MII) in the cold temperature group (53.4%) than in the room and incubator temperature groups (20.0 and 2.4%, respectively). In Experiment 2, ovaries were stored in physiological saline at room temperature for 0, 6, 12 or 18 h, and then they were stored at 4 C (cold storage) until reaching a total storage period of 24 h. After storage of the ovaries, morphologically intact oocytes were matured, fertilized with frozen-thawed spermatozoa, and cultured in vitro. The rates of morphologically intact oocytes obtained from the ovaries stored at room temperature for 0, 6, 12 or 18 h were 35.3, 30.0, 26.4 and 14.7%, respectively, and the rates of intact oocytes that reached MII were 63.2, 36.4, 26.5 and 11.9%, respectively. The results suggested that the numbers of morphologically intact oocytes and intact oocytes that reached MII after in vitro maturation decrease gradually as the period of storage at room temperature before cold storage increases. Only oocytes from ovaries stored for 6 h developed to the blastocyst stage after in vitro maturation and fertilization when ovaries were stored at room temperature before cold storage. These results indicate that 24 h storage of ovaries at high temperatures (>23 C) decreases the meiotic competence of oocytes. The quality and developmental competence of oocytes are influenced by the length of storage at room temperature before cold storage.
Naoi
Hideaki
H
Laboratory of Animal Reproduction, United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
Otoi
Takeshige
T
Shimamura
Takano
T
Karja
Ni Wayan Kurniani
NW
Agung
Budiyanto
B
Shimizu
Ryohei
R
Taniguchi
Masayasu
M
Nagai
Takashi
T
D000818:Animals; D002415:Cats; D002970:Cleavage Stage, Ovum; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D008540:Meiosis; D009865:Oocytes / Q000502:physiology*; D009926:Organ Preservation / Q000379:methods*; D010053:Ovary / Q000166:cytology* / Q000502:physiology; D013696:Temperature; D013997:Time Factors