title: A rapid and simple HPLC method for the analysis of propofol in biological fluids.
abstract: A selective and sensitive high-performance liquid chromatographic method for the analysis of propofol in biological samples was developed. Propofol and thymol (internal standard) were analysed on a Purospher RP-18 endcapped (75 mmx4 mm, 3 microm) stationary phase using acetonitrile and water (65:35, v/v) as eluents at a flow rate of 0.6 mL/min. The excitation and emission wavelengths were 276 and 310 nm, respectively. Sample treatment consisted of deproteinization by acetonitrile containing the internal standard and direct injection of the supernatant. Mean analytical recovery were 105% (CV 2.0%) at concentrations ranging from 0.05 to 10 mg/L. The quantification limit was 3 ng/mL for a 500 microL sample plasma volume and 5 ng/mL for a 500 microL blood sample. The intra-day and inter-day precisions were lower than 5.5% for three concentrations assessed (0.05, 1.0 and 10.0 mg/L). Considering the column size and the flow rate, the separation was achieved with an analysis time less than 6 min with a reduced consumption of solvent. This rapid HPLC method using a simple treatment procedure is sensitive enough for monitoring propofol in human biological samples.
authors.affiliation: Université de Lyon, and Hospices Civils de Lyon, Laboratoire de Pharmacocinétique Clinique, Hôpital Cardiologique, Bron, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Cussonneau
authors.forename: Els
authors.initials: E
authors.lastname: De Smet
authors.forename: Kristof
authors.initials: K
authors.lastname: Lantsoght
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Salvi
authors.forename: Magali
authors.initials: M
authors.lastname: Bolon-Larger
authors.forename: Roselyne
authors.initials: R
authors.lastname: Boulieu
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D018686:Anesthetics, Intravenous / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015742:Propofol / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D018686:Anesthetics, Intravenous; D012997:Solvents; D014867:Water; D015742:Propofol; C032159:acetonitrile