title: In vitro and in vivo activity of SKI-606, a novel Src-Abl inhibitor, against imatinib-resistant Bcr-Abl+ neoplastic cells.
abstract: Resistance to imatinib represents an important scientific and clinical issue in chronic myelogenous leukemia. In the present study, the effects of the novel inhibitor SKI-606 on various models of resistance to imatinib were studied. SKI-606 proved to be an active inhibitor of Bcr-Abl in several chronic myelogenous leukemia cell lines and transfectants, with IC(50) values in the low nanomolar range, 1 to 2 logs lower than those obtained with imatinib. Cells expressing activated forms of KIT or platelet-derived growth factor receptor (PDGFR), two additional targets of imatinib, were unaffected by SKI-606, whereas activity was found against PIM2. SKI-606 retained activity in cells where resistance to imatinib was caused by BCR-ABL gene amplification and in three of four Bcr-Abl point mutants tested. In vivo experiments confirmed SKI-606 activity in models where resistance was not caused by mutations as well as in cells carrying the Y253F, E255K, and D276G mutations. Modeling considerations attribute the superior activity of SKI-606 to its ability to bind a conformation of Bcr-Abl different from imatinib.
authors.affiliation: Department of Clinical Medicine, S. Gerardo Hospital-University of Milano-Bicocca, Monza, Italy.
authors.forename: Miriam
authors.initials: M
authors.lastname: Puttini
authors.forename: Addolorata Maria Luce
authors.initials: AM
authors.lastname: Coluccia
authors.forename: Frank
authors.initials: F
authors.lastname: Boschelli
authors.forename: Loredana
authors.initials: L
authors.lastname: Cleris
authors.forename: Edoardo
authors.initials: E
authors.lastname: Marchesi
authors.forename: Arianna
authors.initials: A
authors.lastname: Donella-Deana
authors.forename: Shaheen
authors.initials: S
authors.lastname: Ahmed
authors.forename: Sara
authors.initials: S
authors.lastname: Redaelli
authors.forename: Rocco
authors.initials: R
authors.lastname: Piazza
authors.forename: Vera
authors.initials: V
authors.lastname: Magistroni
authors.forename: Federica
authors.initials: F
authors.lastname: Andreoni
authors.forename: Leonardo
authors.initials: L
authors.lastname: Scapozza
authors.forename: Franca
authors.initials: F
authors.lastname: Formelli
authors.forename: Carlo
authors.initials: C
authors.lastname: Gambacorti-Passerini
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001549:Benzamides; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D000069439:Dasatinib; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D016044:Fusion Proteins, bcr-abl / Q000235:genetics / Q000378:metabolism; D005838:Genotype; D006801:Humans; D000068877:Imatinib Mesylate; D020014:K562 Cells; D051379:Mice; D008819:Mice, Nude; D008958:Models, Molecular; D009154:Mutation / Q000235:genetics; D009369:Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D009570:Nitriles / Q000737:chemistry / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology*; D011804:Quinolines / Q000737:chemistry / Q000494:pharmacology*; D016019:Survival Analysis; D013844:Thiazoles / Q000494:pharmacology; D020298:U937 Cells; D023041:Xenograft Model Antitumor Assays / Q000379:methods; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; D001549:Benzamides; D009570:Nitriles; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D011743:Pyrimidines; D011804:Quinolines; D013844:Thiazoles; C471992:bosutinib; D000068877:Imatinib Mesylate; D016044:Fusion Proteins, bcr-abl; D019061:src-Family Kinases; D000069439:Dasatinib