Computational studies of ligand-receptor interactions in bitter taste receptors.
Phenylthiocarbamide tastes intensely bitter to some individuals, but others find it completely tasteless. Recently, it was suggested that phenylthiocarbamide elicits bitter taste by interacting with a human G protein-coupled receptor (hTAS2R38) encoded by the PTC gene. The phenylthiocarbamide nontaster trait was linked to three single nucleotide polymorphisms occurring in the PTC gene. Using the crystal structure of bovine rhodopsin as template, we generated the 3D structure of hTAS2R38 bitter taste receptor. We were able to map on the receptor structure the amino acids affected by the genetic polymorphisms and to propose molecular functions for two of them that explained the emergence of the nontaster trait. We used molecular docking simulations to find that phenylthiocarbamide exhibited a higher affinity for the target receptor than the structurally similar molecule 6-n-propylthiouracil, in line with recent experimental studies. A 3D model was constructed for the hTAS2R16 bitter taste receptor as well, by applying the same protocol. We found that the recently published experimental ligand binding affinity data for this receptor correlated well with the binding scores obtained from our molecular docking calculations.
Miguet
Laurence
L
CERMAV BP53, F-38041 Grenoble Cedex 9, France.
Zhang
Ziding
Z
Grigorov
Martin G
MG
D000818:Animals; D002417:Cattle; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D010670:Phenylthiourea / Q000378:metabolism; D011110:Polymorphism, Genetic; D011485:Protein Binding; D011487:Protein Conformation; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism* / Q000502:physiology; D012243:Rhodopsin; D013649:Taste / Q000235:genetics*; D013651:Taste Disorders / Q000235:genetics / Q000378:metabolism
D008024:Ligands; D043562:Receptors, G-Protein-Coupled; D010670:Phenylthiourea; D012243:Rhodopsin