Identification of genes differentially regulated by transcription factor, AP-2delta.
In an effort to better understand the transcriptional program regulated by transcription factor, AP-2delta, we used cDNA microarray to evaluate the relative expression of human genes in AD293 cells by exogenous expression of AP-2delta. Microarray showed 29 genes that were up-regulated and 39 genes with a down-regulated expression pattern. Among the identified genes were those encoding transcription factors, signal transduction molecules, kinases, as well as genes regulating cell growth, differentiation, and cell adhesion, a set of genes suggested to be regulated by AP-2. The results of microarray was validated for some regulated genes by real-time PCR analysis. These observations shed novel insight into the mechanism of AP-2delta action and provide a range of target genes for further investigation.
Sun
Liyun
L
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Huang
Shengdong
S
Wu
Qihan
Q
Gu
Shaohua
S
Fu
Xuping
X
Yu
Ke
K
Lu
Fanglin
F
Ji
Chaoneng
C
Feng
Congjing
C
Sun
Ruping
R
Xie
Yi
Y
Mao
Yumin
Y
D001665:Binding Sites; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050656:Transcription Factor AP-2 / Q000378:metabolism*
D050656:Transcription Factor AP-2