Molecular cloning and nucleotide sequence of CYP6BF1 from the diamondback moth, Plutella xylostella.
A novel cDNA clong encoding a cytochrome P450 was screened from the insecticide-susceptible strain of Plutella xylostella (L.) (Lepidoptera:Yponomeutidae). The nucleotide sequence of the clone, designated CYP6BF1, was determined. This is the first full-length sequence of the CYP6 family from Plutella xylostella (L.). The cDNA is 1661bp in length and contains an open reading frame from base pairs 26 to 1570, encoding a protein of 514 amino acid residues. It is similar to the other insect P450s in gene family 6, including CYP6AE1 from Depressaria pastinacella, (46%). The GenBank accession number is AY971374.
Li
Hongshan
H
Yancheng Academy of Agricultural Sciences, Yancheng, 224002, China. lhs158@sohu.com
Dai
Huaguo
H
Wei
Hui
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D008969:Molecular Sequence Data; D009036:Moths / Q000201:enzymology* / Q000235:genetics*
D003577:Cytochrome P-450 Enzyme System