title: [Preparation of graded zirconia-hydroxyapatite composite bioceramic and its immunocompatibility in vitro].
abstract: Graded Zirconia-hydroxyapatite composite bioceramic and simplex Zirconia-hydroxyapatite composite bioceramic were produced, and the extractes of these two of materials were made to evaluate their immunocompatibility. Scanning electron microscope (SEM) was used to detect the character of the surface of the graded composite bioceramic. Holding and without holding phytohemagglutinin (PHA), proliferation and activation of peripheralblood monocytes(PBMCs) cultured in the two extracts were studied. Cultured in PHA after 72 hours, the proliforation rate of the graded composite group was significantly higher than the simplex composite group (P < 0.01). There was no difference of apoptosis of PBMCs of the two groups (P > 0.05). Cultured in PHA after 24 hours, the ratio of CD3/CD69 positive PBMCs of the simple composite group was significantly higher than that of the graded composite material group (P < 0.01). The numbers of PBMCs activated by the graded composite material group were less than that of the simply composite material group and the technique of graded composite will be helpful to improve its immunocompatibility.
authors.affiliation: Department of Orthopaedics, Second Affiliated Hospital, Zhejiang University, Hangzhou 310009, China. quanrenfu@126.com
authors.forename: Renfu
authors.initials: R
authors.lastname: Quan
authors.forename: Disheng
authors.initials: D
authors.lastname: Yang
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wu
authors.forename: Hongbin
authors.initials: H
authors.lastname: Wang
authors.forename: Xudong
authors.initials: X
authors.lastname: Miao
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D003776:Dental Porcelain / Q000737:chemistry / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D015040:Zirconium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D003776:Dental Porcelain; D017886:Durapatite; D015040:Zirconium; C028541:zirconium oxide