The suppressive effect of resveratrol on protein kinase C theta in peripheral blood T lymphocytes in a rat liver transplantation model.
Our aim was to study the effect of resveratrol on the expressions of protein kinase C isotypes (PKC alpha, theta) in peripheral blood lymphocytes and on the expression of IkappaB kinase-beta (IKK beta) in lymphocytes in allografts in a rat liver transplantation model.


METHODS
Orthotopic liver transplantations (OLT) were performed from Sprague Dawley rats to Wistar rats. The recipients were divided into two groups after OLT. In the RES group, resveratrol was given intraperitoneally once a day (100 mg kg(-1)) after OLT, whereas in the control group vehicle buffer was given. The expressions of PKC alpha, theta in peripheral blood lymphocytes, expression of IKK beta in lymphocytes in allograft, and survival periods were compared between the groups.


RESULTS
The mean survival period after OLT in the RES group was significantly longer than that in control group (P < .05). On posttransplant day 7, the expression of PKC theta in peripheral blood lymphocytes in the RES group was significantly decreased compared with that in the control group (P < .05), whereas there was no obvious difference in the expressions of PKC alpha between the two groups (P > .05), and the positive rate of IKK beta protein in lymphocytes in allografts in RES group was significantly decreased compared with that in the control group (P < .05).


CONCLUSIONS
Resveratrol showed an immunosuppressive effect on lymphocytes for allograft rejection in the rat. Down-regulation of the expression of PKC theta in peripheral blood lymphocytes may be part of the mechanism.
Wu
S-L
SL
Department of General and Transplantation Surgery, University Hospital Essen, Essen, Germany. shengliyili@hotmail.com
Yu
L
L
Jiao
X-Y
XY
Meng
K-W
KW
Pan
C-E
CE
D000818:Animals; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007527:Isoenzymes / Q000235:genetics*; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D011493:Protein Kinase C / Q000235:genetics*; D000074121:Protein Kinase C-theta; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013267:Stilbenes / Q000627:therapeutic use*; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D014665:Vasodilator Agents / Q000627:therapeutic use
D007527:Isoenzymes; D013267:Stilbenes; D014665:Vasodilator Agents; C489591:Prkcq protein, rat; D011493:Protein Kinase C; D000074121:Protein Kinase C-theta; D000077185:Resveratrol