title: Layer-by-layer assembly of polyelectrolyte films improving cytocompatibility to neural cells.
abstract: The using of layer-by-layer assembly polyelectrolyte (PE) films has been suggested as a new versatile technique for surface modification aimed at tissue engineering and cell-based chips. In this study, we investigated the surface morphology of the hyaluronic acid (HA)-based PE films deposited on the amino-functionalized glass slides using atomic force microscopy. These thin films (bilayer number <9) were measured to have nanoscale roughness ranging from 10 to 100 nm. Then the primary hippocampal and cortical neural cells were cultured on the PE films, respectively. After 5 days of culturing, the cytocompatibility to neural cells was evaluated by cellular morphology, neurite outgrowth, and microtubule-associated protein 2 expressions. From the present results, the HA-based PE films were found to be able to support neural cell adhesion and neurite development, especially for the polycation-ending films. It is suggested these HA-based multilayer PE films or similar build-ups could thus be used in the future as a way to modify surfaces for nerve scaffolds and neuron-based chips.
authors.affiliation: Key Laboratory for Oral Biomedical Engineering, Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China.
authors.forename: Zhong-Rong
authors.initials: ZR
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.forename: Bing-Fang
authors.initials: BF
authors.lastname: Liu
authors.forename: Qun-Yuan
authors.initials: QY
authors.lastname: Xu
authors.forename: Fu-Zhai
authors.initials: FZ
authors.lastname: Cui
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004573:Electrolytes / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D036103:Nanotechnology; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D004573:Electrolytes; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D011108:Polymers; D006820:Hyaluronic Acid