Fabrication and application of carbohydrate microarray for analyzing human serum antibody-carbohydrate interaction.
We introduced a strategy for preparing a carbohydrate microarray and demonstrated its utility for characterizing carbohydrate binding and activities. We isolated the lipopolysaccharide (LPS) components from different bacteria and explored the possibility of immobilizing these glycoconjugates on a high-binding polystyrene plate. Carbohydrate-specific combination was examined by observing the binding of the blood group B analogic LPS O-polysaccharide from Escherichia coli on the high-binding polystyrene plate and anti-B from a broad spectra antibody of human blood serum. Strong binding of antibodies was screened, as it was evident that relative response value is two times higher than control. The hybridization results indicated that this method is a reliable technique for the detection of human intestinal bacteria and is expected to be applied in diagnostics and seroepidemiology.
Huang
Gang-Liang
GL
State Key Laboratory of Microbial Technology (SKLMT), Shandong University, Jinan, Shandong, 250100, People's Republic of China. hgl226@126.com.
Mei
Xin-Ya
XY
State Key Laboratory of Microbial Technology (SKLMT), Shandong University, Jinan, Shandong, 250100, People's Republic of China.
Zhang
Han-Xing
HX
State Key Laboratory of Microbial Technology (SKLMT), Shandong University, Jinan, Shandong, 250100, People's Republic of China.
Wang
Peng-George
PG
State Key Laboratory of Microbial Technology (SKLMT), Shandong University, Jinan, Shandong, 250100, People's Republic of China.
D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry; D044967:Serum; D011137:Polystyrenes; D016036:Seroepidemiologic Studies; D002241:Carbohydrates / Q000737:chemistry; D004926:Escherichia coli; D007136:Immunoglobulins
D008070:Lipopolysaccharides; D011137:Polystyrenes; D002241:Carbohydrates; D007136:Immunoglobulins