[The application of large-scale yeast two-hybrid to study protein--protein interaction].
The principle of yeast two-hybrid were summarized, the basis for large scale yeast two-hybrid screening of protein-protein interaction was given and the main application of yeast two-hybrid in large scale screening of protein-protein interaction was presented. The problems of large scale yeast two-hybrid screening of protein-protein interaction were also discussed. Because there were a lot of false positives and false negatives in the result of protein-protein interaction obtained by large-scale yeast two-hybrid screening, the employment of other methods to study protein-protein interaction in large scale in parallel was proposed if possible.
Wu
Zhi-Hao
ZH
Beijing Institute of Radiation Medicine, Beijing 100850, China. wuzh@hupo.org.cn
Wang
Jian
J
He
Fu-Chu
FC
D000818:Animals; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D011506:Proteins