title: Isolation of stress responsive Psb A gene from rice (Oryza sativa l.) using differential display.
abstract: Differential display (DD) experiments were performed on drought-tolerant rice (Oryza sativa L.) genotype N22 to identify both upregulated and downregulated partial cDNAs with respect to moisture stress. DNA polymorphism was detected between drought-stressed and control leaf tissues on the DD gels. A partial cDNA showing differential expression, with respect to moisture stress was isolated from the gel. Northern blotting analysis was performed using this cDNA as a probe and it was observed that mRNA corresponding to this transcript was accumulated to high level in rice leaves under water deficit stress. At the DNA sequence level, the partial cDNA showed homology with psb A gene encoding for Dl protein.
authors.affiliation: Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110 012.
authors.forename: Aruna
authors.initials: A
authors.lastname: Tyagi
authors.forename: Arti
authors.initials: A
authors.lastname: Chandra
mesh_terms: D001483:Base Sequence; D001671:Biochemistry / Q000379:methods; D015152:Blotting, Northern; D002736:Chloroplasts / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D018744:DNA, Plant; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics*; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D045332:Photosystem II Protein Complex