AFLP markers for the R-gene in the flea beetle, Phyllotreta nemorum, conferring resistance to defenses in Barbarea vulgaris.
A so-called R-gene renders the yellow-striped flea beetle Phyllotreta nemorum L. (Coleoptera: Chrysomelidae: Alticinae) resistant to the defenses of the yellow rocket Barbarea vulgaris R.Br. (Brassicacea) and enables it to use it as a host plant in Denmark. In this study, genetic markers for an autosomal R-gene, inherited as a single, dominant locus in flea beetles from the Danish locality "Kvaerkeby" are described, and a genetic linkage map around this particular R-gene is constructed, using the technique of AFLP (Amplified Fragment Length Polymorphism).
Breuker
Casper J
CJ
Section of Evolutionary Biology, Institute of Biology, Leiden University, P.O. Box 9516, 2300 RA Leiden, The Netherlands. casper_j_breuker@yahoo.co.uk
Victoir
Kathleen
K
De Jong
Peter W
PW
van der Meijden
Eddy
E
Brakefield
Paul M
PM
Vrieling
Klaas
K
D000818:Animals; D031216:Barbarea / Q000378:metabolism*; D002874:Chromosome Mapping; D001517:Coleoptera / Q000235:genetics*; D005260:Female; D017344:Genes, Insect / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D008297:Male; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic
D005819:Genetic Markers