title: Detection of Osteopontin in the pericyst of human hepatic Echinococcus granulosus.
abstract: It aims at investigating the expression and distribution of the Osteopontin (OPN) in the pericyst of human hepatic Echinococcus granulosus and their related significances. Sixty pericysts excised by "sub-adventitial cystectomy" were studied. OPN was detected in 80% (48/60) of cysts by Western blotting and distributed in the side of "exocyst" layer directing to the parasite, also macrophages were identified in the vicinity of OPN by immunohistochemistry staining. The coexpression of OPN and CD68 was observed by immunofluorescence double labeling and analyzed by Image-Pro Plus 5.1; with special stain techniques, variable degrees of calcium deposits were observed in 80% (48/60) cysts, and the calcium deposits concurrencely found with the OPN expression. The selective distribution of OPN, calcium in the "exocyst" provides a new pathological evidence for the "sub-adventitial cystectomy" we developed. The pericyst of hepatic E. granulosus consists of two detachable layers with different formative mechanisms: the "exocyst" layer directing towards the cyst of parasite was the result of granulomatous reaction; also the results suggest OPN is one regulator in the granulomatous reaction and calcification of "exocyst".
authors.affiliation: Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China. pengxy2000@yahoo.com
authors.forename: Xinyu
authors.initials: X
authors.lastname: Peng
authors.forename: Jianhui
authors.initials: J
authors.lastname: Li
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Wu
authors.forename: Shijie
authors.initials: S
authors.lastname: Zhang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Niu
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Chen
authors.forename: Jin
authors.initials: J
authors.lastname: Yao
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D015153:Blotting, Western; D002114:Calcinosis; D003560:Cysts / Q000378:metabolism*; D004444:Echinococcosis, Hepatic / Q000378:metabolism*; D048209:Echinococcus granulosus; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000276:immunology / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism*; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D053495:Osteopontin; C067980:CD68 antigen, human; D000097382:CD68 Molecule