[Rapid identification of compounds in cortex moutan by liquid chromatography-tandem mass spectrometry].
OBJECTIVE
To analyze the chemical constituents of cortex moutan by liquid chromatography coupled with electrospray ionization mass spectrometry.


METHODS
An on-line optimized HPLC-DAD/MS2 technique was employed.


RESULTS
In the negative ion detection mode, 38 components such as monoterpene glucosides, galloylglucoses and acetophenones were isolated. Among them, over thirty compounds were identified, including paeonol, paeonilflorin, oxypaeoniflorin, benzoylpaeoniflorin, benzoyloxypaeoniflorin, galloylpaeoniflorin, galloyloxypaeoniflorin, mundanpioside A, C, D, E, H, etc. by the high performance liquid chromatography with diode-array detection in parallel with electrospray ionization and quadrupole-time of flight tandem mass spectrometry (HPLC-DAD/ESI-MS2).


CONCLUSIONS
This method can be used to rapidly determine the constituents of cortex moutan.
Xu
Shun-jun
SJ
Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
Yang
Liu
L
Zhang
Mian
M
Wang
Zheng-tao
ZT
D000098:Acetophenones / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D005960:Glucosides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D039821:Monoterpenes / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D029596:Paeonia / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000098:Acetophenones; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; D010936:Plant Extracts; C038881:moutan cortex; C517303:mudanpioside H; C047593:oxypaeoniflora; C013638:paeonol