title: Pharmacokinetics, tissue distribution, metabolism, and excretion of depside salts from Salvia miltiorrhiza in rats.
abstract: Salviae miltiorrhiza, a traditional Chinese medical herb known as "Danshen," has been widely used in clinics to improve blood circulation, relieve blood stasis, and treat coronary heart disease. Depside salts from S. miltiorrhiza are a novel drug in which magnesium lithospermate B and its analogs are the active components. The pharmacokinetics, tissue distribution, metabolism, and excretion of three of the major components, lithospermic acid B, rosmarinic acid (RA), and lithospermic acid (LA), were studied by liquid chromatography-tandem mass spectrometry following intravenous administration in Sprague-Dawley rats. The elimination half-lives for LSB, RA, and LA were 1.04, 0.75, and 2.0 h, respectively, when 60 mg/kg S. miltiorrhiza depside salts were administrated. The areas under the curve for LSB, RA, and LA were 51.6, 6.6, and 25.2 mg . h/l, respectively, and the values decreased in the individual tissues in the following order: kidney > lung > liver > heart > spleen > brain for LSB; kidney > lung > heart > liver > spleen > brain for RA; and heart > lung > kidney > liver > spleen > brain for LA. After intravenous administration of 60 mg/kg S. miltiorrhiza depside salts, 86% of the LSB was excreted in the bile within 6 h. The main metabolites M1 and M2 were found in the serum. Overall, the results show that depside salts from S. miltiorrhiza are rapidly and widely distributed to tissues after intravenous administration in rats but that they are also rapidly cleared and excreted.
authors.affiliation: State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Yu
authors.forename: Youli
authors.initials: Y
authors.lastname: Lu
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Gu
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Lijiang
authors.initials: L
authors.lastname: Xuan
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000493:pharmacokinetics*; D002934:Cinnamates / Q000493:pharmacokinetics*; D053630:Depsides / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D014018:Tissue Distribution; D000097123:Rosmarinic Acid
chemical_list: D001572:Benzofurans; D002934:Cinnamates; D053630:Depsides; D000097123:Rosmarinic Acid; C046833:lithospermic acid; C076944:salvianolic acid B