Diallyl sulfide induces the expression of nucleotide excision repair enzymes in the breast of female ACI rats.
Diethylstilbestrol (DES) causes DNA adducts resulting in breast cancer, whereas diallyl sulfide (DAS) inhibits cancer formation. We hypothesize that DAS induces the expression of nucleotide excision repair genes. To test this hypothesis, female ACI rats were treated for 4 days with corn oil, DES, DAS, and DAS/DES (50mg/kg). The expression of P53, Gadd45a, PCNA, and DNA polymerase delta was analyzed by real-time PCR. DES decreased the expression of P53, Gadd45a and PCNA. DAS and DAS/DES increased the expression of all four genes. These results suggest that DAS enhances the ability of breast tissue to repair DNA damage thus preventing cancer.
Green
Mario
M
College of Pharmacy, Florida A&M University, 302 West Pershing Street, Tallahassee, FL 32307, United States. mgreen006@hotmail.com
Newell
Oneil
O
Aboyade-Cole
Ayoola
A
Darling-Reed
Selina
S
Thomas
Ronald D
RD
D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001940:Breast / Q000187:drug effects* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics; D004258:DNA Polymerase III / Q000235:genetics; D004260:DNA Repair; D004054:Diethylstilbestrol / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D009687:Nuclear Proteins / Q000235:genetics; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D051381:Rats; D011912:Rats, Inbred ACI; D013440:Sulfides / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D000097642:GADD45 Proteins
D000498:Allyl Compounds; D016588:Anticarcinogenic Agents; D018797:Cell Cycle Proteins; D004258:DNA Polymerase III; D004054:Diethylstilbestrol; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D013440:Sulfides; D016159:Tumor Suppressor Protein p53; C499073:Gadd45a protein, rat; D000097642:GADD45 Proteins; C038491:allyl sulfide