title: [Characterization of the mRNA profile in ejaculated spermatozoa from healthy fertile men].
abstract: OBJECTIVE
  To explore the complexity of mRNA in the ejaculated sperm from healthy fertile men.
  
  
  METHODS
  Semen samples were collected from 10 healthy fathers. The swim-up method was adopted to purify the sperm from possible contamination of somatic cells and the spermatozoal total RNA extracted by Trizol was used for SAGE library analysis.
  
  
  RESULTS
  A totle of 21 052 SAGE raw tags were sequenced from 877 clones and 2 712 unique tags that occurred at least twice in the library were given further analysis. 19.7% of the unique tags had no match in the existing SAGE map, representing novel genes. Molecular function analysis revealed 67% of unique tags related to protein binding or nucleic acid binding categories, 41% to catalytic activity, 13% to message transducer activity, and 10% to transporter, structural and transcription regulator activity, respectively.
  
  
  CONCLUSIONS
  There exists a complex repertoire of mRNAs in the ejaculated spermatozoa from fertile men.
authors.affiliation: School of Medicine, Shanghai Jiao-tong University, Shanghai 200240, China. yangxingzhao2000@yahoo.com
authors.forename: Yang-Xing
authors.initials: YX
authors.lastname: Zhao
authors.forename: Qiao-Li
authors.initials: QL
authors.lastname: Li
authors.forename: Zhao-Xia
authors.initials: ZX
authors.lastname: Wang
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Lian-Yun
authors.initials: LY
authors.lastname: Wang
authors.forename: Zhong-Dong
authors.initials: ZD
authors.lastname: Qiao
mesh_terms: D000328:Adult; D004542:Ejaculation; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D012333:RNA, Messenger