Peptidomics of identified neurons demonstrates a highly differentiated expression pattern of FXPRLamides in the neuroendocrine system of an insect.
FXPRLamides are insect neuropeptides that mediate such diverse functions as pheromone biosynthesis, visceral muscle contraction, and induction of diapause. Although multiple forms occur in every insect studied so far, little is known about a possible functional differentiation and/or differences in the cellular expression pattern of these messenger molecules. In this study, we performed a mass spectrometric survey of all FXPRLamide-expressing neurosecretory neurons in the CNS of Periplaneta americana. That species combines a very well characterized peptidergic system with relatively easy accessible neurosecretory cells suitable for dissection. In addition to the extensive mass spectrometric analyses of single cells, the projection of the FXPRLamide-expressing neurons was studied with three antisera specifically recognizing different FXPRLamides. The following conclusions can be drawn from this first comprehensive peptidomic approach on insect neurons. 1) A high degree of differentiation in the expression of FXPRLamides exists; not fewer then four cell types containing different sets of FXPRLamides were observed. 2) A low level of colocalization with other neuropeptides was found in these neurons. 3) A comparison with FXPRLamide-expressing neurons of other insects shows a high degree of conservation in the localization and projection of these neurons, which is not corroborated by a similar conservation of the corresponding peptide sequences. 4) Although the methods for cell identification, dissection, and sample preparation for mass spectrometry were kept as simple as possible, it was unambiguously shown that this approach is generally suitable for routine analysis of single identified neurons of insects.
Predel
Reinhard
R
Institut für Allgemeine Zoologie und Tierphysiologie, Friedrich-Schiller-Universität, D-07743 Jena, Germany. b6prre@pan.zoo.uni-jena.de
Eckert
Manfred
M
Pollák
Edit
E
Molnár
László
L
Scheibner
Olaf
O
Neupert
Susanne
S
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure; D002490:Central Nervous System / Q000378:metabolism* / Q000648:ultrastructure; D017952:Ganglia, Invertebrate / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D013058:Mass Spectrometry; D046529:Microscopy, Electron, Transmission; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D009479:Neuropeptides / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D009490:Neurosecretory Systems / Q000378:metabolism* / Q000648:ultrastructure; D010526:Periplaneta / Q000378:metabolism* / Q000648:ultrastructure; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D040901:Proteomics / Q000379:methods; D013045:Species Specificity
D009479:Neuropeptides; C079229:pyrokinin