[The interaction of acridine orange with anionic surfactant and its application to the determination of protein].
The change in the UV-absorption spectrum and fluorescence spectrum of acridine orange(AO) due to the addition of surfactant dodecyi benzene sulfonic acid sodium sait (SDBS) and bovine serum albumin(BSA) was studied. Meanwhile, the effects of the in situ formed AOAO dimer in SDBS as a fluorescence probe and BSA were discussed. A new method for the determination of BSA using fluorescence is presented. The results indicate that the method is sensitive and rapid. The linear range of determination is 0-4. 17 x 10(-7) mol x L(-1). The relative standard deviation is 1.9%, and the detection limit is 8.73 x 10(-10) mol x L(-1).
Shi
Yan
Y
Key Laboratory of Food Science of MOE, Nanchang University, Nanchang 330047, China.
Zheng
Wei-Wan
WW
Wu
Xiao-Xing
XX
Zhou
De-Hong
DH
D000165:Acridine Orange / Q000737:chemistry*; D000818:Animals; D000838:Anions; D001557:Benzenesulfonates / Q000737:chemistry*; D002417:Cattle; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature
D000838:Anions; D001557:Benzenesulfonates; D005456:Fluorescent Dyes; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; C001114:dodecylbenzenesulfonic acid; D000165:Acridine Orange