title: Disruption of actin filaments by latrunculin B affects cell wall construction in Picea meyeri pollen tube by disturbing vesicle trafficking.
abstract: The involvement of actin filaments (AFs) in vesicle trafficking, cell wall construction and tip growth was investigated during pollen tube development of Picea meyeri. Pollen germination and tube elongation were inhibited in a dose-dependent manner by the latrunculin B (LatB) treatment. The fine AFs were broken down into disorganized fragments showing a tendency to aggregate. FM4-64 labeling revealed that the dynamic balance of vesicle trafficking was perturbed due to F-actin disruption and the fountain-like cytoplasmic pattern changed into disorganized Brownian movement. The configuration and/or distribution of cell wall components, such as pectins, callose and cellulose, as well as arabinogalactan proteins changed in obvious ways after the LatB application. Fourier transform infrared (FTIR) analysis further established significant changes in the chemical composition of the wall material. Our results indicate that depolymerization of AFs affects the distribution and configuration of cell wall components in Picea meyeri pollen tube by disturbing vesicle trafficking.
authors.affiliation: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Chen
authors.forename: Nianjun
authors.initials: N
authors.lastname: Teng
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wu
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Jozef
authors.initials: J
authors.lastname: Samaj
authors.forename: Frantisek
authors.initials: F
authors.lastname: Baluska
authors.forename: Jinxing
authors.initials: J
authors.lastname: Lin
mesh_terms: D000199:Actins / Q000378:metabolism*; D002473:Cell Wall / Q000502:physiology* / Q000648:ultrastructure; D002482:Cellulose / Q000378:metabolism; D018525:Germination; D015388:Organelles / Q000502:physiology; D010368:Pectins / Q000378:metabolism; D028222:Picea / Q000254:growth & development / Q000502:physiology*; D010940:Plant Proteins / Q000378:metabolism*; D011058:Pollen / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D021381:Protein Transport / Q000502:physiology; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000199:Actins; D010940:Plant Proteins; D010368:Pectins; D002482:Cellulose