Protective activity of andrographolide and arabinogalactan proteins from Andrographis paniculata Nees. against ethanol-induced toxicity in mice.
To find out the active principles against ethanol-induced toxicity in mice, Andrographis paniculata Nees. (Ap) was chosen and isolated andrographolide (ANDRO) and arabinogalactan proteins (AGPs). ANDRO was detected by HPTLC, FTIR and quantified by HPLC (10mg/g of Ap powder). AGPs was detected by beta-glucosyl Yariv staining of SDS-PAGE gel, FTIR and quantified by single radial gel diffusion assay with beta-glucosyl Yariv reagent (0.5mg/g Ap powder). The mice are pretreated intra-peritoneally (i.p.) with different doses (62.5, 125, 250, and 500mg/kg) of body weight of mice] of ANDRO and AGPs for 7 days and then ethanol (7.5g/kg of body weight) was injected, i.p. Besides, silymarin was used as standard hepatoprotective agent for comparative study with ANDRO and AGPs. The ameliorative activity of ANDRO and AGP against hepatic renal alcohol toxicity was measured by assessing GOT, GPT, ACP, ALP and LP levels in liver and kidney. It has been observed that pretreatment of mice with ANDRO and AGPs at 500mg/kg of body weight and 125mg/kg of body weight respectively could able to minimize the toxicity in compare to ethanol treated group as revealed by the different enzymatic assay in liver and kidney tissues and the results were comparable with silymarin. Hence, out of several ill-defined compounds present in Ap, ANDRO and AGPs are the potential bioactive compounds responsible for protection against ethanol-induced toxicity.
Singha
Prajjal K
PK
Microbiology and Immunology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, West Bengal, India.
Roy
Somenath
S
Dey
Satyahari
S
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D035685:Andrographis / Q000737:chemistry; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002492:Central Nervous System Depressants; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol; D005685:Galactans / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides; D007194:India; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects; D008108:Liver Diseases, Alcoholic / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D051379:Mice; D010696:Phloroglucinol / Q000031:analogs & derivatives; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D012838:Silymarin / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared
D002492:Central Nervous System Depressants; D004224:Diterpenes; D005685:Galactans; D005960:Glucosides; D020011:Protective Agents; D012838:Silymarin; C104977:Yariv reagent; D000431:Ethanol; C030419:andrographolide; D010696:Phloroglucinol; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; C005653:arabinogalactan