CHO glycosylation mutants: proteoglycans.
Most glycosaminoglycan (GAG)-defective mutants have been isolated and characterized from Chinese hamster ovary (CHO) cells. Wild-type and GAG-defective CHO cells have been used by several hundreds of laboratories to study how altering the GAG structure of proteoglycans affects fundamental properties of cells, such as bacterial/viral infection, signaling, protein degradation, and cell adhesion. This chapter describes methods used to construct and characterize new CHO cell lines with gain-of-function GAG structures. These novel CHO cell lines allow herpes simplex virus (HSV) entry or have anticoagulant properties that are not possessed by wild-type CHO cells. The method used to study GAG biosynthetic mechanisms that control specific GAG sequence assembly is also described.
Zhang
Lijuan
L
Washington University School of Medicine, St. Louis, MO, USA.
Lawrence
Roger
R
Frazier
Beth A
BA
Esko
Jeffrey D
JD
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D006025:Glycosaminoglycans / Q000096:biosynthesis* / Q000235:genetics*; D006031:Glycosylation; D009154:Mutation; D011509:Proteoglycans / Q000096:biosynthesis* / Q000235:genetics*
D006025:Glycosaminoglycans; D011509:Proteoglycans