Proteomic analysis of log to stationary growth phase Lactobacillus plantarum cells and a 2-DE database.
Lactobacillus plantarum is part of the natural microbiota of many food fermentations as well as the human gastro-intestinal tract. The cytosolic fraction of the proteome of L. plantarum WCFS1, whose genome has been sequenced, was studied. 2-DE was used to investigate the proteins from the cytosolic fraction isolated from mid- and late-log, early- and late-stationary phase cells to generate reference maps of different growth conditions offering more knowledge of the metabolic behavior of this bacterium. From this fraction, a total of 200 protein spots were identified by MALDI-MS and a proteome production map was constructed to facilitate further studies such as detection of suitable biomarkers for specific growth conditions. More than half (57%) of the identified proteins were predicted to be involved in metabolic pathways of the bacterium. The protein profile changed during the growth of the bacteria such that 29% of the identified proteins involved in anabolic pathways were at least twofold up-regulated throughout the mid- and late-exponential and early-stationary phases. In the late-stationary phase, six proteins involved in stress or with a potential role for survival during starvation were up-regulated significantly.
Cohen
David P A
DP
Laboratory of Microbiology, Wageningen University and Research Center, Wageningen, The Netherlands. David.Cohen@wur.nl
Renes
Johan
J
Bouwman
Freek G
FG
Zoetendal
Erwin G
EG
Mariman
Edwin
E
de Vos
Willem M
WM
Vaughan
Elaine E
EE
D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D048191:Lactiplantibacillus plantarum / Q000254:growth & development* / Q000378:metabolism*; D040901:Proteomics / Q000379:methods* / Q000706:statistics & numerical data; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins