[The study of separating murine embryonic fibroblast from embryonic stem cells by the differential adhesion method].
This study has established a simple method of separating murine embryonic fibroblasts (MEF) from embryonic stem cells (ES cells). The adhesion rates of MEF and ES cells were compared to find out the opitimum time of removing MEF. The result shows that 1.5h and 0.5% gelatin concentration is the opitimum condition of removing MEF. Furthermore, the ES cells which have been purified have the same plating efficiency and the ability of three germ layers differentiation as the unpurified ES cells. After differential adhension, there is strong ALP activity in ES cells. All the results show that the purified ES cells are still in the state of undifferentiation and maintain the pluripotent.
Xu
Chao
C
College of Life Sciences, Zhengjiang University, Hangzhou 310012.
Huang
Hua Rong
HR
Yu
Hong
H
Zhao
Xiao Li
XL
Lu
Yong Liang
YL
Dai
Li Cheng
LC
Yao
Xing
X
D000818:Animals; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology*; D053595:Embryonic Stem Cells / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology