title: The cell membrane-shielding function of eicosapentaenoic acid for Escherichia coli against exogenously added hydrogen peroxide.
abstract: The colony-forming ability of catalase-deficient Escherichia coli mutant genetically modified to produce eicosapentaenoic acid (EPA) showed less decrease than in a control strain producing no EPA, when treated with 0.3mM hydrogen peroxide (H(2)O(2)) under non-growth conditions. H(2)O(2)-induced protein carbonylation was enhanced in cells lacking EPA. The amount of fatty acids was decreased more significantly for cells lacking EPA than for those producing EPA. Much lower intracellular concentrations of H(2)O(2) were detected for cells with EPA than those lacking EPA. These results suggest that cellular EPA can directly protect cells against oxidative damage by shielding the entry of exogenously added H(2)O(2).
authors.affiliation: Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Nishida
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Orikasa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Watanabe
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Okuyama
mesh_terms: D002462:Cell Membrane / Q000187:drug effects*; D015169:Colony Count, Microbial; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D006861:Hydrogen Peroxide / Q000633:toxicity*; D050050:Protein Carbonylation / Q000187:drug effects
chemical_list: D015118:Eicosapentaenoic Acid; D006861:Hydrogen Peroxide