title: In vitro and in vivo anthelmintic activity of crude extracts of Coriandrum sativum against Haemonchus contortus.
abstract: In vitro anthelmintic activities of crude aqueous and hydro-alcoholic extracts of the seeds of Coriandrum sativum (Apiaceae) were investigated on the egg and adult nematode parasite Haemonchus contortus. The aqueous extract of Coriandrum sativum was also investigated for in vivo anthelmintic activity in sheep infected with Haemonchus contortus. Both extract types of Coriandrum sativum inhibited hatching of eggs completely at a concentration less than 0.5 mg/ml. ED(50) of aqueous extract of Coriandrum sativum was 0.12 mg/ml while that of hydro-alcoholic extract was 0.18 mg/ml. There was no statistically significant difference between aqueous and hydro-alcoholic extracts (p>0.05). The hydro-alcoholic extract showed better in vitro activity against adult parasites than the aqueous one. For the in vivo study, 24 sheep artificially infected with Haemonchus contortus were randomly divided into four groups of six animals each. The first two groups were treated with crude aqueous extract of Coriandrum sativum at 0.45 and 0.9 g/kg dose levels, the third group with albendazole at 3.8 mg/kg and the last group was left untreated. Efficacy was tested by faecal egg count reduction (FECR) and total worm count reduction (TWCR). On day 2 post treatment, significant FECR was detected in groups treated with higher dose of Coriandrum sativum (p<0.05) and albendazole (p<0.001). On days 7 and 14 post treatment, significant FECR was not detected for both doses of Coriandrum sativum (p>0.05). Significant (p<0.05) TWCR was detected only for higher dose of Coriandrum sativum compared to the untreated group. Reduction in male worms was higher than female worms. Treatment with both doses of Coriandrum sativum did not help the animals improve or maintain their PCV while those treated with albendazole showed significant increase in PCV (p<0.05).
authors.affiliation: Ethiopian Institute of Agricultural Research, National Animal Health Research Center, P.O. Box 04, Sebeta, Ethiopia. tadesseeguale@yahoo.com
authors.forename: T
authors.initials: T
authors.lastname: Eguale
authors.forename: G
authors.initials: G
authors.lastname: Tilahun
authors.forename: A
authors.initials: A
authors.lastname: Debella
authors.forename: A
authors.initials: A
authors.lastname: Feleke
authors.forename: E
authors.initials: E
authors.lastname: Makonnen
mesh_terms: D015766:Albendazole / Q000627:therapeutic use; D000470:Alkaloids / Q000737:chemistry / Q000627:therapeutic use; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000627:therapeutic use*; D028526:Coriandrum / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000469:parasitology; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000627:therapeutic use; D006188:Haemonchiasis / Q000188:drug therapy* / Q000662:veterinary; D006189:Haemonchus / Q000187:drug effects; D006400:Hematocrit / Q000662:veterinary; D008297:Male; D010270:Parasite Egg Count / Q000662:veterinary; D008517:Phytotherapy / Q000662:veterinary; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use*; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D012639:Seeds; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy* / Q000469:parasitology
chemical_list: D000470:Alkaloids; D000871:Anthelmintics; D005419:Flavonoids; D010936:Plant Extracts; D015766:Albendazole