Development of a glucose-6-phosphate biosensor based on coimmobilized p-hydroxybenzoate hydroxylase and glucose-6-phosphate dehydrogenase.
This work reports the development of an amperometric glucose-6-phosphate biosensor by coimmobilizing p-hydroxybenzoate hydroxylase (HBH) and glucose-6-phosphate dehydrogenase (G6PDH) on a screen-printed electrode. The principle of the determination scheme is as follows: G6PDH catalyzes the specific dehydrogenation of glucose-6-phosphate by consuming NADP(+). The product, NADPH, initiates the irreversible the hydroxylation of p-hydroxybenzoate by HBH in the presence of oxygen to produce 3,4-dihydroxybenzoate, which results in a detectable signal due to its oxidation at the working electrode. The sensor shows a broad linear detection range between 2 microM and 1000 microM with a low detection limit of 1.2 microM. Also, it has a fast measuring time which can achieve 95% of the maximum current response in 20s after the addition of a given concentration of glucose-6-phosphate with a short recovery time (2 min).
Cui
Yue
Y
Department of Chemical Engineering and Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. kecuiyue@ust.hk <kecuiyue@ust.hk>
Barford
John P
JP
Renneberg
Reinhard
R
D015109:4-Hydroxybenzoate-3-Monooxygenase / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003067:Coenzymes / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D019298:Glucose-6-Phosphate / Q000032:analysis* / Q000737:chemistry; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003067:Coenzymes; D004800:Enzymes, Immobilized; D019298:Glucose-6-Phosphate; D005954:Glucosephosphate Dehydrogenase; D015109:4-Hydroxybenzoate-3-Monooxygenase