Effect of exercise training on in vitro LDL oxidation and free radical-induced hemolysis: the HERITAGE Family Study.
Oxidant stress and overproduction of reactive oxygen species (ROS) contribute to the development of cardiovascular disease. Oxidative modifications of low-density lipoproteins (LDL) are thought to play an early and critical role in atherogenesis. LDL oxidation can be reproduced in vitro, but results usually show a large interindividual variation not entirely explained by the environment. Free radical-induced hemolysis is also proposed to reveal the overall antioxidant capacity. The roles of genetic factors and exercise on the variability of both measures were investigated. The study was conducted in 146 healthy individuals from 28 families participating in a 20-week exercise-training program. In addition to important biological and environmental influences on variation, significant familial aggregation was detected in all oxidation measures. Exercise did not significantly modify the LDL oxidation parameters, but significantly increased resistance was observed in the free radical-induced hemolysis, especially in women, this effect was not observed in smokers. In total, the findings suggest the presence of familial effects in the response to ex vivo oxidation. Further, smoking negates the beneficial effect of exercise training on erythrocyte resistance to free radical-induced hemolysis. These observations emphasize the importance of context in the evaluation of exercise and oxidant stress.
Blache
Denis
D
INSERM U 498, Biochimie des Lipoprotéines et Interactions Vasculaires, and Faculté de Médecine, Université de Bourgogne, Dijon, France. dblache@u-bourgogne.fr
Lussier-Cacan
Suzanne
S
Gagnon
Jacques
J
Leon
Arthur S
AS
Rao
D C
DC
Skinner
James S
JS
Wilmore
Jack H
JH
Rankinen
Tuomo
T
Bouchard
Claude
C
Davignon
Jean
J
D000293:Adolescent; D000328:Adult; D016021:Epidemiologic Studies; D004912:Erythrocytes; D015444:Exercise; D005260:Female; D005609:Free Radicals / Q000494:pharmacology*; D006461:Hemolysis; D006801:Humans; D008055:Lipids; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction
D005609:Free Radicals; D008055:Lipids; D008077:Lipoproteins, LDL