Deletion and recombination events between the DNA-A and DNA-B components of Indian cassava-infecting geminiviruses generate defective molecules in Nicotiana benthamiana.
Cloned DNA-B components, belonging to the bipartite begomoviruses Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV), family Geminiviridae, when co-inoculated along with previously cloned DNA-A components of the respective viruses onto the experimental host Nicotiana benthamiana, generated defective DNAs (def-DNA) ranging in size from 549 to 1555 nucleotides. All the cloned def-DNAs contained the common region (CR) as well as portions of either DNA-A or DNA-B and, in a few cases, both DNA-A and DNA-B, representing recombinant products, the junction points of which correspond to repeats of 2-11 bases found in the parental molecules. The DNA-B-derived def-DNAs were, in some cases, associated with a decrease in levels of DNA-B, with a concomitant change in the symptoms from downward leaf curling in the older leaves to upward leaf-rolling in newly emerging leaves, more typical of monopartite begomoviruses.
Patil
Basavaprabhu L
BL
University of Delhi South Campus, Department of Plant Molecular Biology, New Delhi 110021, India.
Dutt
Nitin
N
Briddon
Rob W
RW
Bull
Simon E
SE
Rothenstein
Dirk
D
Borah
Basanta K
BK
Dasgupta
Indranil
I
Stanley
John
J
Jeske
Holger
H
D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D017791:Geminiviridae / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D002365:Manihot / Q000821:virology; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000235:genetics*; D014026:Nicotiana / Q000821:virology
D004279:DNA, Viral