title: Incidence and distribution pattern of Rickettsia felis in peridomestic fleas from Andalusia, Southeast Spain.
abstract: The presence of Rickettsia felis was investigated in three species of pulicid fleas (Ctenocephalides felis, Ctenocephalides canis and Pulex irritans) collected in 38 locales in Andalusia (Spain) over the period 1999-2004. Amplification of a fragment of OmpB gene was positive in 54.17 % of lots of Ct. felis. The identity of the PCR bands was confirmed as R. felis by sequence data obtained directly from the PCR amplicon. No rickettsia was found in Ct. canis nor P. irritans.
authors.affiliation: Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Parage Las Lagunillas s/n, 23071-Jaén, Spain. jmarquez@ujaen.es
authors.forename: F J
authors.initials: FJ
authors.lastname: Márquez
authors.forename: M A
authors.initials: MA
authors.lastname: Muniain
authors.forename: J J
authors.initials: JJ
authors.lastname: Rodríguez-Liebana
authors.forename: M D
authors.initials: MD
authors.lastname: Del Toro
authors.forename: M
authors.initials: M
authors.lastname: Bernabeu-Wittel
authors.forename: A J
authors.initials: AJ
authors.lastname: Pachón
mesh_terms: D000818:Animals; D005843:Geography; D006801:Humans; D015994:Incidence; D012282:Rickettsia Infections / Q000145:classification / Q000453:epidemiology*; D041161:Rickettsia felis; D005423:Siphonaptera / Q000145:classification / Q000382:microbiology*; D013030:Spain / Q000453:epidemiology