Clinical features and hormonal profiles of cloprostenol-induced early abortions in heifers monitored by ultrasonography.
BACKGROUND
The present study describes the clinical features and plasma profiles of bovine pregnancy-associated glycoprotein 1 (bPAG1), the main metabolite of prostaglandin F2alpha (PG metabolite) and progesterone (P4) in heifers in which early abortions were induced.


METHODS
Early abortions were induced in four heifers with cloprostenol and monitored by ultrasonography. Blood samples were collected and the plasma were analyzed for bPAG 1, P4 and PG metabolite.


RESULTS
The foetal heartbeat rates varied from 170-186 beats per minute for all foetuses up to the date of cloprostenol treatment. Foetal death was confirmed within two days after cloprostenol treatment. Prior to cloprostenol injection, blood plasma concentrations of bPAG1, PG metabolite and P4 varied from 8.4-40.0 ng/mL, 158-275 pmol/L and 20.7-46.9 nmol/L, respectively. After the foetus expelled, the plasma level of bPAG1 began to decrease but the decrease was small and gradual. The estimated half-life of bPAG1 was 1.8-6.6 days. The plasma level of the PG metabolite started to have short lasting peaks (above 300 pmol/L) within three hours after cloprostenol treatment. The plasma concentrations of P4 dropped sharply to less than 4 nmol/L after 24 hours of cloprostenol injection.


CONCLUSIONS
The current findings indicated that after early closprostenol-induced foetal death, the plasma concentration of bPAG1 decreased gradually and showed a tendency of variation with the stages of pregnancy.
Lobago
Fikre
F
Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Box 7054, SE-750 07, Uppsala, Sweden. fikre_lobago@yahoo.com <fikre_lobago@yahoo.com>
Gustafsson
Hans
H
Bekana
Merga
M
Beckers
Jean-François
JF
Kindahl
Hans
H
D000028:Abortion, Induced / Q000379:methods / Q000662:veterinary*; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000097:blood*; D002417:Cattle / Q000502:physiology*; D003008:Cloprostenol / Q000008:administration & dosage / Q000494:pharmacology*; D015237:Dinoprost / Q000097:blood; D005260:Female; D005313:Fetal Death / Q000097:blood / Q000139:chemically induced* / Q000000981:diagnostic imaging; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood*; D011374:Progesterone / Q000097:blood; D013997:Time Factors; D014463:Ultrasonography / Q000662:veterinary
D011257:Pregnancy Proteins; D003008:Cloprostenol; D011374:Progesterone; D015237:Dinoprost; D016282:Aspartic Acid Endopeptidases; C094766:pregnancy-associated glycoprotein 1