title: Efficient purification of transglutaminase from recombinant Streptomyces platensis at various scales.
abstract: An efficient system for the fast and efficient purification of transglutaminase from recombinant Streptomyces platensis and expressed in Streptomyces lividans 25-2 is described. Because the purification procedure of this system is flexible, culture broth from laboratory (20 l) and pilot-plant (130 l) fermentations were used to purify the enzyme to electrophoretic homogeneity with high purity (90-95%) and yield (61-77%) within 1 or 2 days.
authors.affiliation: Food Industry Research and Development Institute, Hsinchu, Taiwan, ROC. lsj@firdi.org.tw
authors.forename: Shie-Jea
authors.initials: SJ
authors.lastname: Lin
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Hsieh
authors.forename: Pei-Ming
authors.initials: PM
authors.lastname: Wang
authors.forename: Wen-Shen
authors.initials: WS
authors.lastname: Chu
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D024861:Genetic Enhancement / Q000379:methods*; D010865:Pilot Projects; D011995:Recombination, Genetic / Q000235:genetics; D013302:Streptomyces / Q000201:enzymology* / Q000378:metabolism*; D011503:Transglutaminases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D011503:Transglutaminases