The N-terminal domain of Bcl-xL reversibly binds membranes in a pH-dependent manner.
Bcl-xL regulates apoptosis by maintaining the integrity of the mitochondrial outer membrane by adopting both soluble and membrane-associated forms. The membrane-associated conformation does not require a conserved, C-terminal transmembrane domain and appears to be inserted into the bilayer of synthetic membranes as assessed by membrane permeabilization and critical surface pressure measurements. Membrane association is reversible and is regulated by the cooperative binding of approximately two protons to the protein. Two acidic residues, Glu153 and Asp156, that lie in a conserved hairpin of Bcl-xLDeltaTM appear to be important in this process on the basis of a 16% increase in the level of membrane association of the double mutant E153Q/D156N. Contrary to that for the wild type, membrane permeabilization for the mutant is not correlated with membrane association. Monolayer surface pressure measurements suggest that this effect is primarily due to less membrane penetration. These results suggest that E153 and D156 are important for the Bcl-xLDeltaTM conformational change and that membrane binding can be distinct from membrane permeabilization. Taken together, these studies support a model in which Bcl-xL activity is controlled by reversible insertion of its N-terminal domain into the mitochondrial outer membrane. Future studies with Bcl-xL mutants such as E153Q/D156N should allow determination of the relative contributions of membrane binding, insertion, and permeabilization to the regulation of apoptosis.
Thuduppathy
Guruvasuthevan R
GR
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Terrones
Oihana
O
Craig
Jeffrey W
JW
Basañez
Gorka
G
Hill
R Blake
RB
D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability; D017124:Conserved Sequence; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011312:Pressure; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012996:Solutions; D051020:bcl-X Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D012996:Solutions; D051020:bcl-X Protein