title: Isolation of an isoflavone-metabolizing, Clostridium-like bacterium, strain TM-40, from human faeces.
abstract: Recently, the biological effects of isoflavones have attracted much attention. Intestinal microbiota plays an important role in the metabolism and bioavailability of isoflavones. However, few reports have discussed intestinal bacteria that metabolize daidzein into dihydrodaidzein. In this study, we isolated the dihydrodaidzein-producing intestinal bacterium TM-40 from a healthy boy's faeces. The bacteria from faecal samples were incubated with daidzein. Among all tested bacteria, one strain (strain TM-40) produced dihydrodaidzein both from daidzein and daidzin. However, in our experimental conditions, strain TM-40 did not produce equol from daidzein. The 16S rRNA partial sequence of strain TM-40 (AB249652) exhibited a 93% similarity to that of Coprobacillus catenaformis (AB030218). This strain seems to be a new species.
authors.affiliation: National Food Research Institute, Tsukuba 305-8642, Japan.
authors.forename: Motoi
authors.initials: M
authors.lastname: Tamura
authors.forename: Tojiro
authors.initials: T
authors.lastname: Tsushida
authors.forename: Kazuki
authors.initials: K
authors.lastname: Shinohara
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002648:Child; D003013:Clostridium / Q000145:classification / Q000235:genetics; D005243:Feces / Q000382:microbiology; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D007529:Isoflavones / Q000378:metabolism*; D008297:Male; D010802:Phylogeny
chemical_list: D007529:Isoflavones; C433163:dihydrodaidzein; C004742:daidzein