Isolation and characterization of acidic glycosphingolipids from the gill of the Pacific Salmon (Oncorhynchus keta): a novel hybrid-type ganglioside with isoglobo- and neolacto-Series.
Monosialosyl gangliosides and sulfoglycolipids in the gill of pacific salmon, Oncorhynchus keta, have been prepared by solvent extraction and DEAE-Sephadex column chromatography. Acidic glycolipid bands (M1-M13) detected by thin layer chromatography were separated by Iatrobeads column chromatography and 13 components were characterized by TLC, compositional analysis, methylation analysis, chemical and enzymatic degradation, liquid secondary ion mass spectrometry and (1)H nuclear magnetic resonance spectroscopy. In addition to the acidic glycolipids with known structures (SM4s, SM3, GM3, LM1, GM1b and V(3)alphaFuc,IV(3)betaGalNAc-GM1a), two fractions (M11 and M13) of unknown monosialosyl gangliosides with TLC mobility slower than GM1a were isolated and characterized as having the following structure with a hybrid of isoglobo- and neolacto-series. [formula: see text] Analysis of fatty acid indicated predominance of C24:1 fatty acid in the upper band (M11) and shorter chain saturated fatty acids in the lower band (M13). The tissue concentrations of M11 and M13 were 1.15 and 0.96 mumol/kg wet weight, respectively.
Niimura
Yukio
Y
Research Center of Biomedical Analysis and Radioisotope, Teikyo University School of Medicine, Tokyo, Japan. yniimura@med.teikyo-u.ac.jp
D020384:Acidic Glycosphingolipids / Q000737:chemistry* / Q000302:isolation & purification*; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D005880:Gills / Q000737:chemistry*; D006017:Glycolipids / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D017685:Oncorhynchus keta; D018629:Spectrometry, Mass, Secondary Ion
D020384:Acidic Glycosphingolipids; D006017:Glycolipids; C011117:sulfoglycolipids