title: Changes in growth hormone (GH) messenger RNA (GH mRNA) expression in the rat anterior pituitary after single interferon (IFN) alpha administration.
abstract: BACKGROUND
  Interferon alpha (IFN-alpha) is a cytokine with pleiotropic effects which, via different pathways, influences the secretion of certain cytokines and hormones. Growth hormone (GH) secreted from the pituitary has physiological effects on various target tissues. The question is how IFN-alpha administered in various types of disease influences GH secretion. This study investigated the acute effect of IFN-alpha on GH mRNA expression in the rat anterior pituitary.
  
  
  OBJECTIVE
  The aim of the study was to measure the cellular expression of GH mRNA by in situ hybridisation in the anterior pituitary after a single administration of IFN-alpha.
  
  
  METHODS
  Rats were administered an intraperitoneal injection of IFN-alpha or saline. The rat pituitaries were taken 2 and 4 hours after IFN/saline administration and kept frozen until in situ hybridisation histochemistry. A 31-base(35)S-labelled oligonucleotide probe complementary to part of the exonic mRNA sequence coding for GH mRNA was used. All control and experimental sections were hybridised in the same hybridisation reaction.
  
  
  RESULTS
  Acute administration of interferon alpha increased GH mRNA expression in the anterior pituitary in the 4-hour group in comparison with the control group, and there was no difference between the control group and the 2-hour rats.
  
  
  CONCLUSIONS
  A single IFN-alpha administration was found to exert an influence on anterior pituitary GH mRNA expression. These observations may pave the way for presenting a possible new action of IFN-alpha.
authors.affiliation: Department of Internal Medicine, Bytom, Silesian Medical University, Katowice, Poland. jacek_roma@poczta.onet.pl
authors.forename: Wojciech
authors.initials: W
authors.lastname: Romanowski
authors.forename: Ryszard
authors.initials: R
authors.lastname: Braczkowski
authors.forename: Janusz
authors.initials: J
authors.lastname: Kosiewicz
authors.forename: Ilona
authors.initials: I
authors.lastname: Korzonek
authors.forename: Ewa
authors.initials: E
authors.lastname: Nowakowska-Zajdel
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Muc-Wierzgoń
authors.forename: Barbara
authors.initials: B
authors.lastname: Zubelewicz-Szkodzińska
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects*; D013006:Growth Hormone / Q000235:genetics*; D017403:In Situ Hybridization; D007274:Injections, Intraperitoneal; D016898:Interferon-alpha / Q000008:administration & dosage*; D008297:Male; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D016898:Interferon-alpha; D012333:RNA, Messenger; D013006:Growth Hormone