title: Golgin-160 promotes cell surface expression of the beta-1 adrenergic receptor.
abstract: Golgin-160 is a ubiquitously expressed peripheral Golgi membrane protein that is important for transduction of certain pro-apoptotic signals at the Golgi complex. However, the role of golgin-160 in normal Golgi structure and function is unknown. Here, we show that depletion of golgin-160 using RNA interference (RNAi) does not affect Golgi morphology or constitutive membrane traffic in HeLa cells. However, depletion of golgin-160 leads to significantly decreased cell surface levels of exogenously expressed beta1-adrenergic receptor (beta1AR), which can be rescued by expression of RNAi-resistant forms of golgin-160. Furthermore, overexpression of golgin-160 leads to higher surface levels of beta1AR. Golgin-160 is localized mostly in the cis and medial regions of the Golgi stack by immunoelectron microscopy, suggesting that it does not directly promote incorporation of beta1AR into transport vesicles at the trans Golgi network. Golgin-160 interacts with beta1AR in vitro, and we mapped the interaction to a region between residues 140 and 257 in the head of golgin-160 and the third intracellular loop of beta1AR. Our results support the idea that golgin-160 may promote efficient surface delivery of a subset of cargo molecules.
authors.affiliation: Department of Cell Biology, Johns Hopkins University School of Medicine, 725 Wolfe Street, Baltimore, MD 21205, USA.
authors.forename: Stuart W
authors.initials: SW
authors.lastname: Hicks
authors.forename: Troy A
authors.initials: TA
authors.lastname: Horn
authors.forename: J Michael
authors.initials: JM
authors.lastname: McCaffery
authors.forename: David M
authors.initials: DM
authors.lastname: Zuckerman
authors.forename: Carolyn E
authors.initials: CE
authors.lastname: Machamer
mesh_terms: D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism / Q000648:ultrastructure; D000077614:Golgi Matrix Proteins; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D011485:Protein Binding; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000235:genetics; D018342:Receptors, Adrenergic, beta-1 / Q000378:metabolism*
chemical_list: D001324:Autoantigens; C108388:GOLGA3 protein, human; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; D034741:RNA, Small Interfering; D018342:Receptors, Adrenergic, beta-1