title: Inhibitory effects of different antioxidants on hyaluronan depolymerization.
abstract: Hyaluronan (HA) was depolymerized by hydroxyl radicals generated from hydrogen peroxide and cupric ions. Inhibition of HA degradation by four well-known antioxidants was investigated, as HA can scavenge reactive oxygen species (ROS). Change in hyaluronan molecular weight was observed by size-exclusion chromatography. Inhibition of HA degradation was estimated from the retention times observed. It was found that HA degradation was inhibited in a clearly concentration-dependent manner by mannitol, thiourea and vinpocetine. Propofol also inhibited the depolymerization, but its concentration-dependent effect was not so clear. The antioxidant concentrations at which HA degradation was decreased by 50% were 42 microM for thiourea; 1.35 microM for vinpocetine; and 0.39 microM for propofol. A concentration of 26.51 mM of mannitol was needed to attain the same inhibitory effect. Although many factors are involved in a therapeutic response, the results obtained in this study support the idea that HA may be protected from ROS attack by the concomitant use of well-known antioxidants.
authors.affiliation: Department of Physiology, University of Leon, E-24071 Leon, Spain.
authors.forename: Gracia
authors.initials: G
authors.lastname: Mendoza
authors.forename: Ana I
authors.initials: AI
authors.lastname: Alvarez
authors.forename: Mivis M
authors.initials: MM
authors.lastname: Pulido
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Molina
authors.forename: Gracia
authors.initials: G
authors.lastname: Merino
authors.forename: Rebeca
authors.initials: R
authors.lastname: Real
authors.forename: Peter
authors.initials: P
authors.lastname: Fernandes
authors.forename: Julio G
authors.initials: JG
authors.lastname: Prieto
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000494:pharmacology; D008353:Mannitol / Q000494:pharmacology; D008970:Molecular Weight; D011108:Polymers / Q000378:metabolism*; D015742:Propofol / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000494:pharmacology; D013890:Thiourea / Q000494:pharmacology; D014748:Vinca Alkaloids / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D011108:Polymers; D017382:Reactive Oxygen Species; D014748:Vinca Alkaloids; D017665:Hydroxyl Radical; D008353:Mannitol; C013983:vinpocetine; D006820:Hyaluronic Acid; D006861:Hydrogen Peroxide; D013890:Thiourea; D015742:Propofol