Investigations of differences in iron oxidation state inside single neurons from substantia nigra of Parkinson's disease and control patients using the micro-XANES technique.
X-ray absorption near edge structure spectroscopy was applied in order to investigate differences in iron chemical state between the nerve cells of substantia nigra (SN) representing Parkinson's disease (PD) and those of control cases. Autopsy samples were cut using a cryotome, and were not fixed and not embedded in paraffin. The comparison of the absorption spectra near the iron K-edge measured in melanized neurons from SN of PD and control samples did not show significant differences in iron oxidation state. Measurements of inorganic reference materials containing iron in the second and third oxidation states indicate that most of the iron in all the nerve cell bodies examined was oxidized and occurred as trivalent ferric iron (Fe(3+)).
Chwiej
Joanna
J
Department of Nuclear Methods, Faculty of Physics and Applied Computer Science, AGH-University of Science and Technology, Al. Mickiewicza 30, 30-059, Cracow, Poland. chwiej@novell.ftj.agh.edu.pl
Adamek
Dariusz
D
Szczerbowska-Boruchowska
Magdalena
M
Krygowska-Wajs
Anna
A
Wojcik
Slawomir
S
Falkenberg
Gerald
G
Manka
Anna
A
Lankosz
Marek
M
D006801:Humans; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D010084:Oxidation-Reduction; D010300:Parkinson Disease / Q000378:metabolism* / Q000473:pathology; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D013194:Staining and Labeling; D013378:Substantia Nigra / Q000737:chemistry / Q000166:cytology / Q000378:metabolism* / Q000473:pathology
D007501:Iron