title: Periprostatic tissues intraoperative frozen section during retrograde radical retropubic prostatectomy.
abstract: OBJECTIVE
  To evaluate usefulness of periprostatic tissues intraoperative frozen sections (PTs IFSs) during RRP for prostate cancer, in order to find local extraprostatic neoplastic spreading and to eventually modify intervention and resection limits during surgery.
  
  
  METHODS
  From January 1998 to June 2004, 259 consecutive patients underwent RRP at our department for clinically organ-confined prostate cancer; PTs IFSs were prospectively performed in all cases at membranous urethra after removal of prostatic apex, at whole neurovascular bundle (NVB) or at fibroadipose tissue subtended between prostatic capsule and NVB during extrafascial or nerve sparing (NS) RRP respectively, at middle portion of Denonvillier's fascia, at detrusor ring after removal of the prostate. IFSs positivity was followed by further excision at the corresponding site during intervention. RRP pathological specimen was handled and examined according to European Association of Urology (EAU) guidelines. Student's t-test and chi-square test were used for statistic analysis, matching patients with or without positive PTs IFSs for bioptic Gleason sum, preoperative serum PSA, clinical stage and lymph nodal involvement.
  
  
  RESULTS
  PTs IFSs were positive 75 times in 63 patients out of 259. Pathological stage considering PTs IFSs overlapped 2002 TNM definitive pathological stage in 228 patients. The remaining 31 cases showed PTs neoplastic involvement at IFSs. These latter patients did not show prostatic capsular infiltration at definitive pathology. We demostrated intraoperative extraprostatic cancer spreading that was unrecognizable at definitive pathology. PTs neoplastic spreading changed NS RRP in extrafascial procedure in 17 patients out of 121 with preoperative planned NS RRP There were no significant statistic differences between patients with or without positive periprostatic margins (PMs), regarding preoperative serum PSA, bioptic Gleason sum and clinical stage (Student's t-test); lymph nodal involvement rate was not significantly different in both groups (chi-square test).
  
  
  CONCLUSIONS
  PTs IFSs during RRP were feasible and effective in order to achieve better local pathological staging in 12% of patients, to modify planned nerve sparing RRP in extrafascial procedure in 14% of cases and to wide intraoperatively surgical resection margins in 24% of patients.
authors.affiliation: Department of Urology, Ospedale San Lazzaro, Alba, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Fasolis
authors.forename: Paolo
authors.initials: P
authors.lastname: Degiuli
authors.forename: Mauro
authors.initials: M
authors.lastname: Lancia
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Fasolo
authors.forename: Enrico
authors.initials: E
authors.lastname: Conti
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Sebastiani
authors.forename: Sergio
authors.initials: S
authors.lastname: Lacquaniti
mesh_terms: D000368:Aged; D005629:Frozen Sections; D006801:Humans; D007432:Intraoperative Period; D008297:Male; D011446:Prospective Studies; D011467:Prostate / Q000473:pathology*; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000601:surgery*