Studying drug-plasma protein interactions by two-injector microchip electrophoresis frontal analysis.
We developed a simple, rapid, and sensitive two-injector microchip electrophoresis frontal analysis (MCE-FA) method for studying drug-plasma protein interactions. In this method, large volumes of a reference sample and drug-plasma protein mixture were simultaneously introduced into the respective sections of the microchannel through the separated injectors and then electrophoresed. Since the reference sample did not meet with the interacting species during migration, it could be used as an external standard. The interaction between heparin and HSA was quantitatively characterized as a model system. The binding constant was found to be (1.53 +/- 0.01) x 10(4) M(-1).
Liu
Xiaojun
X
Dalian institute of Chemical Physics, Chinese Academy of Sciences, Dalian, PR China.
Liang
Aiye
A
Shen
Zheng
Z
Liu
Xin
X
Zhang
Yu
Y
Dai
Zhongpeng
Z
Xiong
Bohui
B
Lin
Bingcheng
B
D000925:Anticoagulants / Q000097:blood / Q000378:metabolism; D004341:Drug Evaluation / Q000295:instrumentation / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D017022:Flow Injection Analysis; D006493:Heparin / Q000097:blood / Q000378:metabolism; D006801:Humans; D004364:Pharmaceutical Preparations / Q000097:blood / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*
D000925:Anticoagulants; D004364:Pharmaceutical Preparations; D012709:Serum Albumin; D006493:Heparin