Identification of non-specific alkaline phosphatases in hyphal cells of the fungus Neurospora crassa by in situ histochemistry.
The present study was designed to identify alkaline phosphatases in non-permeabilized hyphal cells of the fungus Neurospora crassa by staining these enzymatic activities with a modified azo dye coupling method. Our strategy allowed the identification of three non-specific alkaline phosphatase activities, one of them possibly being a novel putative enzyme, which is not responsive to either Mg(2+) or EDTA. Another alkaline phosphatase activity, whose location in the hyphal cell is regulated by phosphate, is stimulated by Mg(2+), inhibited by EDTA, and somehow dependent on the expression of the pho-2(+) -encoded Pi-repressible alkaline phosphatase.
Thedei
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Pró-Reitoria de Pesquisa, Pós-Graduação e Extensão, Universidade de Uberaba, Uberaba, MG, Brasil.
Rossi
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A
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000235:genetics; D002462:Cell Membrane; D004492:Edetic Acid; D006651:Histocytochemistry; D025301:Hyphae / Q000201:enzymology*; D009492:Neurospora crassa / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D013194:Staining and Labeling; D013997:Time Factors
D004492:Edetic Acid; D000469:Alkaline Phosphatase