Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family.
Matrine, one of the main active components from the dry roots of Sophora flavescence, was known to induce apoptosis in a variety of tumor cells in vitro. However, the molecular mechanism of cell apoptosis induced by Matrine remains elusive. Here, we investigated the apoptosis in Matrine-treated human gastric cancer MKN45 cells. The results showed that Matrine could inhibit cell proliferation and induce apoptosis in a dose-dependent manner. Further immunoblots revealed that in Matrine-treated cells, caspase-3, -7 were activated and the pro-apoptotic molecules Bok, Bak, Bax, Puma, and Bim were also up-regulated. Our results suggested that Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family.
Luo
Cong
C
Cancer Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.
Zhu
Yongliang
Y
Jiang
Tiejun
T
Lu
Xiaoyong
X
Zhang
Wo
W
Jing
Qifeng
Q
Li
Jia
J
Pang
Linrong
L
Chen
Kangjie
K
Qiu
Fuming
F
Yu
Xiuyan
X
Yang
Jiahui
J
Huang
Jian
J
D000470:Alkaloids / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011807:Quinolizines / Q000633:toxicity*; D013274:Stomach Neoplasms; D000093842:Matrines
D000470:Alkaloids; D019253:Proto-Oncogene Proteins c-bcl-2; D011807:Quinolizines; D053148:Caspase 3; D053179:Caspase 7; D000093842:Matrines