[Simultaneous determination of total flavone and total saponin in Gynostemma pentaphyllum by signal multiplier spectrophotometry].
Important chemical constituents analysis for the total flavone and total saponin in Gynostemma pentaphyllum is described. The colour reactions of flavones and saponines with vanillin-perchloric acid in acetic acid produced the good absorptions at 451 and 547 nm, but the absorption peaks too overlapped to be determined simultaneously. A new method for the total flavone and the total saponin in Gynostemma pentaphyllum to be determined by signal multiplier spectrophotometry simultaneously without any preliminary separation was proposed. For quantitative analysis, the rutinum as a standard of the total flavone and the ginsenoside Rb1 as standard of the total saponin were applied. The experiment results showed that the regression equations of concentration and deltaA were obtained: deltaAflavone = 0.0133+4.417 0Cflavone, relation coefficient rflavone = 0.9994, and the total flavone concentrations were in 0-0. 16 microg x mI(-1) with deltaA obeying linear relation; deltaAsaporin = 2.775 5Csaponin -0.8881 x 10(-2), relation coefficient rsaponin = 0.9991, and the total saponin concentrations were in 0-0.30 microg x mL(-1) with deltaA obeying linear relation respectively. The recovery ratio was 104.0%-113.0% and 86.8%-94.6% respectively. The RSDflavone was less than 0.58% (n = 9) and RSDsaponin was less than 0. 35% (n=9) respectively. The proposed method is simple, rapid accurate and feasible.
Hui
Rui-Hua
RH
Department of Chemistry, Anshan Normal University, Anshan 114005, China.
Hou
Dong-Yan
DY
Li
Tie-Chun
TC
Liu
Xiao-Yuan
XY
D005240:Feasibility Studies; D047309:Flavones; D005419:Flavonoids / Q000032:analysis*; D036145:Ginsenosides / Q000032:analysis; D029774:Gynostemma / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods*
D047309:Flavones; D005419:Flavonoids; D036145:Ginsenosides; D012503:Saponins; C442759:ginsenoside Rb1; C043562:flavone