title: Protection of Procambarus clarkii against white spot syndrome virus using recombinant oral vaccine expressed in Pichia pastoris.
abstract: The potential for oral vaccination of crayfish against white spot syndrome virus was investigated. The envelope proteins VP19 and VP28 were expressed in yeast (Pichia pastoris). The expressed proteins were used as oral vaccines in different forms viz., in whole culture form, whole culture sonicated form, whole culture centrifuged supernatant form, and cell residue form. The recombinant proteins were mixed with food pellets and fed to crayfish for 25 days. The vaccinated groups were divided into two even groups and challenged on the 3rd and 21st day of post vaccination. Among different vaccine groups the relative percent survival (RPS) values of sonicated form and supernatant form vaccines were found the best and met the criterion (>RPS 60%) of effective vaccine even after 21st day of post vaccination. Development of vaccine by using recombinant proteins VP19 and VP28 in yeast as expression vector was feasible with significant effects.
authors.affiliation: Department of Animal Biotechnology, Genetic Engineering Laboratory, Feed Science Institute, Zhejiang University, 164, Qiu Tao Bei Lu, Hangzhou 310029, China. rajeev@zju.edu.cn
authors.forename: Rajeev Kumar
authors.initials: RK
authors.lastname: Jha
authors.forename: Zi Rong
authors.initials: ZR
authors.lastname: Xu
authors.forename: Shi Juan
authors.initials: SJ
authors.lastname: Bai
authors.forename: Jian Yu
authors.initials: JY
authors.lastname: Sun
authors.forename: Wei Fen
authors.initials: WF
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003400:Astacoidea / Q000821:virology*; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000235:genetics; D010843:Pichia / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D016019:Survival Analysis; D013997:Time Factors; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D046848:White spot syndrome virus 1 / Q000276:immunology*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; C446941:VP19 protein, white spot syndrome virus; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines