title: MEKC: an update focusing on practical aspects.
abstract: This paper reviews recent methodological and instrumental advances in MEKC. Improvements in sensitivity arising from the use of on-line sample concentration (sweeping, stacking, and combination of both protocols) and derivatization (in-capillary reactions and coupling with flow-injection systems) and improvements in resolution obtained by changing the composition of the BGE (e.g., with organic modifiers, ionic liquids, nonionic and zwitterionic surfactants, mixed micelles, and vesicles) or using coated capillaries are discussed in detail. In addition, MS and LIF spectroscopy are examined in relation to their advantages and restrictions as applied to MEKC analysis. Some thoughts on potential future directions are also expressed.
authors.affiliation: Department of Analytical Chemistry, University of Cordoba, Cordoba, Spain. qa1sirom@uco.es
authors.forename: Manuel
authors.initials: M
authors.lastname: Silva
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D004573:Electrolytes / Q000737:chemistry; D005470:Fluorometry; D013058:Mass Spectrometry; D012680:Sensitivity and Specificity; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D004573:Electrolytes; D013501:Surface-Active Agents