Long-term feeding a high-fat diet causes histological and parasitological effects on murine schistosomiasis mansoni outcome.
This study investigated whether long-term feeding a high-fat diet (HFC) has an effect on schistosomiasis mansoni outcome compared to standard chow diet (SC). Swiss Webster female mice (3 wk old) fed each diet over 5 months, and then were infected with 50 Schistosoma mansoni cercariae. Their nutritional status was assessed by monitoring growth rates twice a week and measuring serum levels of lipoproteins. Mice were euthanised 63 days after infection. Parasitological and liver histological analyses were performed. The levels of TC, HDL-C and LDL-C, fecal and tissue schistosome eggs were statistically different (p<0.05) between groups. Livers from HFC mice showed exudative, exudative/exudative-productive, exudative-productive and productive granulomas, some degree of hepatic steatosis and focal necrosis. Mice fed normal-chow did not present productive granulomas and hepatic steatosis. The morphometric evaluation of hepatic granulomas did not reach statistical significance (p>0.05) between diets assayed. The high-fat diet for long-term produces effects on schistosomiasis mansoni outcome.
Neves
Renata Heisler
RH
Laboratory of Helminthology Romero Lascasas Porto, Biomedical Center, Department of Pathology and Laboratories, Faculty of Medical Sciences, State University of Rio de Janeiro, Brazil.
Miranda de Barros Alencar
Alba Cristina
AC
Costa-Silva
Michele
M
Aguila
Márcia Barbosa
MB
Mandarim-de-Lacerda
Carlos Alberto
CA
Machado-Silva
José Roberto
JR
Gomes
Delir Corrêa
DC
D000818:Animals; D002784:Cholesterol / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects*; D004195:Disease Models, Animal; D005243:Feces / Q000469:parasitology; D005260:Female; D006099:Granuloma / Q000209:etiology / Q000473:pathology; D008099:Liver / Q000469:parasitology / Q000473:pathology; D051379:Mice; D009765:Obesity / Q000150:complications*; D010270:Parasite Egg Count; D012550:Schistosoma mansoni / Q000254:growth & development; D012555:Schistosomiasis mansoni / Q000209:etiology* / Q000473:pathology
D004041:Dietary Fats; D002784:Cholesterol