title: Fish-isolated Naegleria strains and their phylogeny inferred from ITS and SSU rDNA sequences.
abstract: Effort was made to identify Naegleria strains isolated from organs of fish, using phylogenetic analyses of SSU rDNA and ITS sequences. Eighteen fish-isolated strains studied enlarged substantially the so far available set of Naegleria strains characterized by both molecular markers. The phylogenetic analyses of separate and concatenated SSU rDNA and ITS sequences revealed phylogenetic relationships of strains under study; however, they failed to solve classification of fish-isolated strains into species. The sequence similarity of strain-representatives of Naegleria species as well as data obtained on intragenomic variation of ITS sequences discouraged the authors from the definition of new species. The results of the present study provide evidence of a need to re-evaluate the current practice of setting boundaries between species of the genus Naegleria. Sequences obtained in this study have been deposited in GenBank with accession numbers DQ768714-DQ768743.
authors.affiliation: Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic. iva@paru.cas.cz
authors.forename: Iva
authors.initials: I
authors.lastname: Dyková
authors.forename: Hana
authors.initials: H
authors.lastname: Pecková
authors.forename: Ivan
authors.initials: I
authors.lastname: Fiala
authors.forename: Helena
authors.initials: H
authors.lastname: Dvoráková
mesh_terms: D000562:Amebiasis / Q000469:parasitology*; D000818:Animals; D001483:Base Sequence; D005393:Fish Diseases / Q000469:parasitology*; D005399:Fishes / Q000469:parasitology*; D008969:Molecular Sequence Data; D009253:Naegleria / Q000145:classification / Q000235:genetics*; D010802:Phylogeny