Mixing two differently labeled substrates in one immobilized metal assay for phosphochemicals assay to improve data quality.
Jezequel-Sur
Sylvie
S
Department of Automated Biotechnology, Merck & Company, North Wales, PA 19454, USA. sylvie_sur@merck.com
Murray
Justin
J
Lucumi
Edinson
E
Ferrer
Marc
M
Zuck
Paul
P
D000595:Amino Acid Sequence; D002232:Carbocyanines / Q000737:chemistry; D000071877:Checkpoint Kinase 1; D031541:Fluorescence Resonance Energy Transfer; D008670:Metals / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D011494:Protein Kinases / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry; D013379:Substrate Specificity
C114557:5-carboxytetramethylrhodamine succinimidyl ester; D002232:Carbocyanines; D008670:Metals; D010455:Peptides; D010710:Phosphates; D012235:Rhodamines; C085321:cyanine dye 5; D011494:Protein Kinases; D000071877:Checkpoint Kinase 1