A rational approach to minimal high-resolution cross-reactive arrays.
We report a rational approach to the construction of cross-reactive arrays for steroids consisting of five to seven sensors incorporating modified oligonucleotides. The sensors for our arrays were selected to maximize their differential responses to the two steroids most different in an arbitrarily chosen parameter named "shape-length". The arrays incorporated three previously unreported types of sensors identified through a massive screening effort: (1) three-way junction sensors with neutralized charges within junction; (2) "self-aggregating sensors"; and (3) sensors incorporating fluorophore directly in a three-way junction as a spacer. The arrays were tested on seven steroids and an alkaloid (cocaine) over a range of concentrations, and achieved 92-96% accuracy in class assignments, despite the close structural similarities between steroids.
Green
Eric
E
Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, New York, New York 10032, USA.
Olah
Mark J
MJ
Abramova
Tatiana
T
Williams
Lance R
LR
Stefanovic
Darko
D
Worgall
Tilla
T
Stojanovic
Milan N
MN
D015374:Biosensing Techniques / Q000379:methods*; D003429:Cross Reactions; D005456:Fluorescent Dyes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013256:Steroids / Q000032:analysis* / Q000737:chemistry
D005456:Fluorescent Dyes; D009841:Oligonucleotides; D013256:Steroids