title: [Resistance to HIV-1 infection of CD4 + T lymphocytes in vitro from chinese HIV-1 exposed seronegative individuals].
abstract: OBJECTIVE
  To determine the relative resistance to HIV-1 infection of CD4 + T lymphocytes in HIV-exposed seronegative individuals (ESNs) in China.
  
  
  METHODS
  HIV primary isolates were obtained from peripheral whole blood of HIV-infected persons. CD4 + T lymphocytes of Chinese ESNs were separated from peripheral blood mononuclear cells with magnetic cell sorting (MACS). The purified CD4 + T lymphocytes were cocultured with HIV primary isolates. The p24 level was detected and the culture medium was refreshed every 3 days within 2 weeks.
  
  
  RESULTS
  For M tropic HIV strains, p24 level was significantly lower in ESN group than in control group (P < 0.05); for some M tropic HIV strains, even no p24 replicated in ESN group. However, T tropic virus strains had no significant difference between these two groups (P > 0.05).
  
  
  CONCLUSIONS
  CD4 + T lymphocytes of Chinese ESNs may possess relative resistance to M tropic HIV strains, which may be one of the main influencing factors that result in ESN.
authors.affiliation: Center for AIDS Research, the First Affiliated Hospital, China Medical University, Shenyang 110001, China.
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Su
authors.forename: Hong
authors.initials: H
authors.lastname: Shang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Shu-xiang
authors.initials: SX
authors.lastname: Wang
authors.forename: Wen-qing
authors.initials: WQ
authors.lastname: Geng
authors.forename: Hua-lu
authors.initials: HL
authors.lastname: Cui
authors.forename: Yong-jun
authors.initials: YJ
authors.lastname: Jiang
authors.forename: Ya-nan
authors.initials: YN
authors.lastname: Wang
authors.forename: Zi-ning
authors.initials: ZN
authors.lastname: Zhang
authors.forename: Ya-ting
authors.initials: YT
authors.lastname: Wang
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D002681:China; D005260:Female; D006678:HIV / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity; D015658:HIV Infections / Q000821:virology*; D018023:HIV Seronegativity / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D012747:Sexual Partners