Effectiveness of short-contact topical tretinoin in promoting wound healing in db/db mice.
We have investigated the effects of short-contact topical application of tretinoin using 16 male db/db mice by creating two full-thickness wounds on the skin of the back. A 0.1% tretinoin aqueous gel was applied to one wound for five minutes daily for five successive days while only aqueous gel was applied to the other (control). The mean (SD) percentage surface area unhealed before and after treatment were 0.88 (0.3) and 0.64 (0.15). In tretinoin-treated mice and non-treated mice (controls), the mean (SD) thicknesses of granulation were 1.383 (697) micro m and 683 (413) micro m, the density of capillary vessels in granulation was 12.2 (5.5)% and 5.7 (3.9)%, respectively. Differences between the two groups were significant for each variable. Wound healing was accelerated with short-contact topical application of tretinoin in db/db mice.
Toyama
Takuya
T
Department of Plastic and Reconstructive Surgery, Kyorin University School of Medicine, Tokyo, Japan. takuya@kyorin-u.ac.jp
Ohura
Norihiko
N
Kurita
Masakazu
M
Momosawa
Akira
A
Harii
Kiyonori
K
D000818:Animals; D005782:Gels; D006097:Granulation Tissue / Q000473:pathology; D007641:Keratolytic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D014212:Tretinoin / Q000008:administration & dosage / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D005782:Gels; D007641:Keratolytic Agents; D014212:Tretinoin