title: A cleavable amino-thiol linker for reversible linking of amines to DNA.
abstract: A cleavable heterobifunctional cross-linker for the reversible conjugation of amines to thiol-modified DNA has been developed and tested. The succinimidyl 2-(vinylsulfonyl)ethyl carbonate (SVEC) was prepared in three steps and tested for its ability to react with amines and thiols. The linker was efficient for binding leucine to a thiol-modified DNA sequence and for releasing the amino acid at pH 11.8.
authors.affiliation: Center for Catalysis and Interdisciplinary Nanoscience Center (iNANO), Department of Chemistry, University of Aarhus, Langelandsgade 140, 8000 Aarhus C, Denmark.
authors.forename: Jonas W
authors.initials: JW
authors.lastname: Højfeldt
authors.forename: Peter
authors.initials: P
authors.lastname: Blakskjaer
authors.forename: Kurt V
authors.initials: KV
authors.lastname: Gothelf
mesh_terms: D000588:Amines / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000588:Amines; D013438:Sulfhydryl Compounds; D004247:DNA