Morphology of the lateral pterygoid muscle associated to the mandibular condyle in the human prenatal stage.
The lateral pterygoid muscle (LPM) inserts at the condyle and the articular disc and plays a central role in mandibular movement via the Temporomandibular Articular Complex. The aim of this study was to examine the association between the morphology of LPM muscular fascicles and the degree of mineralization of the mandibular condyle in the prenatal stage employing structural, ultrastructural and microanalytical evaluation. Sixteen human fetuses at 11-37 weeks of gestation, with no apparent pathology and resulting from spontaneous abortions, were included in the study. Samples from lateral pterygoid muscle and the mandibular condyle were processed for light microscopy and electron microscopy and microanalysis. Desmin immunolabeling (dilution 1: 25 Dako) and alpha sarcomeric actin immunolabeling (dilution 1:50 Dako) employing the avidin-biotin system were used in paraffin embedded samples. Contralateral samples were examine by transmission electron microscopy. Four condyles (at 17-21 weeks of gestation) were used to measure the relative content of calcium and phosphorous employing the X-ray diffraction microanalytical technique. At 11-16 weeks of gestation, the LPM was composed of secondary myotubes associated to satellite cells and nerve fibers. At 18 weeks, the muscle exhibited multiple compact fascicles and the condyle showed a thin, external, subperiostal mineralized layer with few central bone spicules. At 20 weeks, at the site of insertion of the LPM, the bone trabeculae of the condyle contained an electrondense matrix with abundant mineralization nuclei. At 17-21 weeks of gestation no significant variations in the contents of phosphorous and calcium were observed. At 24 weeks, transmission electron calcium and microscopy studies revealed a marked increase in the functional units of the muscle fascicles. Also, at this age muscle fibers exhibited differences in the expression of desmin and alpha sarcomeric actin. At 37 weeks the muscle became multipennate in appearance, exhibiting a more complex organization than younger fetuses. Alpha sarcomeric actin labeling became light with age. This results suggest that between 16 and 22 weeks of gestation the differentiation and maturation process of the muscle fibers precedes and prevails over the development and mineralization process from mandibular condyle. The rudimentary performance of the prenatal LPM would be one of the factors that regulate the process of ossification at the level of the mandibular condyle. The rate of ossification would increase starting from 22 of gestation week.
Carranza
Miriam L
ML
Department of Oral Biology, Histology, Faculty of Dentistry, National University of Córdoba, Argentina.
Carda
Carmen
C
Simbrón
Alicia
A
Quevedo
María C Sánchez
MC
Celaya
Gabriela
G
de Ferraris
Maria Elsa Gómez
ME
D000199:Actins / Q000032:analysis; D002113:Calcification, Physiologic; D002118:Calcium / Q000032:analysis; D003893:Desmin / Q000032:analysis; D005333:Fetus; D005865:Gestational Age; D006801:Humans; D008335:Mandibular Condyle / Q000196:embryology*; D009024:Morphogenesis; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal; D010758:Phosphorus / Q000032:analysis; D011626:Pterygoid Muscles / Q000196:embryology*; D018709:Statistics, Nonparametric; D019224:Temporomandibular Joint Disc / Q000196:embryology
D000199:Actins; D003893:Desmin; D010758:Phosphorus; D002118:Calcium