The effects of hypoxia-ischemia on neutral amino acid transporters in the developing rat brain.
The neutral amino acid transporters SNAT1-3 and ASCT1 play critical roles in the recycling of glutamine, and subsequently glutamate, via the glutamine-glutamate cycle. Hypoxia-ischemia was induced in rat pups using the Rice-Vannucci model. Brains were harvested at 1 h, 24 h and 7 days after ischemia. The expression of NAATs was evaluated using immunoblotting, real-time PCR, and immunohistochemistry. Results were compared with age-matched controls and shams. SNAT1 mRNA decreased at 1 h after injury in both hemispheres when compared with the control animals and correlated with a decrease in protein expression at 24 h in the hippocampus and cortex. SNAT1 protein expression increased globally at 7 days after injury and specifically in the hippocampus. Finally, SNAT2 and 3 demonstrated subtle changes in various brain regions after injury. These data suggest that neutral amino acid transporters remain largely intact after hypoxia-ischemia.
Leibovici
Avital
A
Department of Pediatrics, University of Florida, Gainesville, FL 32610-0296, USA. leiboa@peds.ufl.edu
Rossignol
Candace
C
Montrowl
Jennifer A
JA
Erickson
Jeffery D
JD
Varoqui
Helene
H
Watanabe
Masahiko
M
Chaudhry
Farrukh A
FA
Bredahl
May Kristin L
MK
Anderson
Kevin J
KJ
Weiss
Michael D
MD
D026924:Amino Acid Transport System A / Q000235:genetics / Q000378:metabolism*; D026962:Amino Acid Transport System ASC / Q000235:genetics / Q000378:metabolism; D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics / Q000378:metabolism; D021542:Amino Acids, Neutral / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism* / Q000503:physiopathology*; D015151:Immunoblotting; D007150:Immunohistochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
D026924:Amino Acid Transport System A; D026962:Amino Acid Transport System ASC; D026905:Amino Acid Transport Systems; D026921:Amino Acid Transport Systems, Neutral; D021542:Amino Acids, Neutral; C420504:Slc1a4 protein, rat; C486716:Slc38a1 protein, rat; C412568:Slc38a2 protein, rat; C403222:system N protein 1