Use of molecular markers and flow cytometry to preserve ancient Annurca apple germplasm.
The old Annurca apple cultivar (Malus domestica), particularly appreciated for its peculiar flavor and crispy flesh, was studied in order to preserve its ancient germplasm. Twelve clones of Annurca were analyzed using random amplified polymorphic DNA (RAPD) and simple-sequence repeat (SSR) markers. Two out of 30 RAPD primers and nine out of ten SSR primers were able to discriminate all the clones analyzed. Data were confirmed by measuring DNA content using flow cytometry. The results provide a good procedure to improve germplasm field management, in order to removing redundant material in the Annurca collection. This represents an efficient way to create a data bank in order to preserve the genetic variability of the Annurca cultivar.
Iannaccone
Marco
M
School of Biotechnological Sciences, University of Naples Federico II, Portici, Naples, Italy.
Palumbo
Daniela
D
Ventimiglia
Ilaria
I
Patocchi
Andrea
A
Spigno
Paola
P
Capparelli
Rosanna
R
D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry / Q000379:methods*; D005638:Fruit / Q000235:genetics*; D014644:Genetic Variation; D027845:Malus / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods
D017931:DNA Primers; D018744:DNA, Plant