Vaccination with a recombinant chicken FGFR-1 bypasses immunological tolerance against self-FGFR-1 in mice.
The possibility that a recombinant protein vaccine based on xenogeneic homologous FGFR-1 of chicken induces production of autoantibodies against self-FGFR-1 in BALB/c mice was examined by using ELISA, Western blot analysis and ELISPOT assay respectively. Autoantibodies against mouse FGFR-1 were identified by Western blot analysis and ELISA. Compared with the two control groups, the number of APBCs, which were detected by ELISPOT assay, was significantly increased in the spleens of mice immunized with cFR1 (P < 0.05). IgG1 and IgG2b, which were detected by ELISA, were the major subclasses and were substantially increased in response to chicken FGFR-1 when compared with control group. The recombinant chicken FGFR-1 protein used as a vaccine can induce autoantibodies against self-FGFR-1 in mice and provide a basis for the active immunotherapy of tumor angiogenesis.
Zheng
Shaojiang
S
Department of Pathology, School of Basic Science, Tongji Medical College, Huazhong University Science and Technology, Wuhan 430030, China.
Huang
Fengying
F
Zheng
Shaoping
S
Wang
Wei
W
Yin
Hui
H
Wu
Renliang
R
D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D002645:Chickens; D005260:Female; D007108:Immune Tolerance / Q000276:immunology; D016233:Immunotherapy, Active / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use
D001323:Autoantibodies; D019496:Cancer Vaccines; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D051496:Receptor, Fibroblast Growth Factor, Type 1