RNA interference of Hemolin causes depletion of phenoloxidase activity in Hyalophora cecropia.
Melanization is regulated by the prophenoloxidase cascade and functions as a response to intruding microorganisms in invertebrates. When injecting dsRNA of the lepidopteran immune protein hemolin in pupae of Hyalophora cecropia (Lepidoptera: Saturniidae), we observed a significant reduction in phenoloxidase activity after 24 h, but not after 72 h. The link between hemolin and the prophenoloxidase system suggests that hemolin is a pattern recognition protein important for the triggering of the prophenoloxidase cascade in the defence against bacterial infections.
Terenius
Olle
O
Department of Genetics, Microbiology and Toxicology, Stockholm University, 106 91 Stockholm, Sweden.
Bettencourt
Raul
R
Lee
So Young
SY
Li
Wenli
W
Söderhäll
Kenneth
K
Faye
Ingrid
I
D000818:Animals; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007915:Lepidoptera / Q000201:enzymology* / Q000276:immunology*; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D034622:RNA Interference / Q000276:immunology*
D007136:Immunoglobulins; D019476:Insect Proteins; C066846:hemolin; D014442:Monophenol Monooxygenase