title: Adenoviral sensors for high-content cellular analysis.
abstract: To maximize the potential of high-content cellular analysis for investigating complex cellular signaling pathways and processes, we have generated a library of adenoviral encoded cellular sensors based on protein translocation and reporter gene activation that enable a diverse set of assays to be applied to lead compound profiling in drug discovery and development. Adenoviral vector transduction is an efficient and technically simple system for expression of cellular sensors in diverse cell types, including primary cells. Adenoviral vector-mediated transient expression of cellular sensors, either as fluorescent protein fusions or live cell gene reporters, allows rapid assay development for profiling the activities of candidate drugs across multiple cellular systems selected for biological and physiological relevance to the target disease state.
authors.affiliation: GE Healthcare, the Maynard Centre, Forest Farm, Whitchurch, Cardiff, UK.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Kendall
authors.forename: Ray
authors.initials: R
authors.lastname: Ismail
authors.forename: Nick
authors.initials: N
authors.lastname: Thomas
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D006224:Cricetinae; D015195:Drug Design; D004345:Drug Industry / Q000379:methods*; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D021381:Protein Transport; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D011965:Receptors, Glucocorticoid; D049452:Green Fluorescent Proteins