[Isoflavone accumulation associated with cell structural changes in Maackia amurensis suspension cultures elicited by methyl jasmonate, salicylic acid and nitric oxide].
Comparison between changes in isoflavone accumulation and cell structure in Maackia amurensis suspension cultures elicited by methyl jasmonate (MeJA),salicylic acid (SA) and nitric oxide (NO) was studied. The results suggested that MeJA, SA and NO can all stimulate isoflavone production remarkably. After 9d treatment with 200 micromol/L MeJA,100 micromol/L SA and 50 micromol/L SNP, the isoflavone content was 417.18%, 185.45% and 222.45% of the control, respectively. At the same time, the electron-dense body (EDB) could be easily found in the cells, while its number achieved most after 9 d treatment,and the number of EDB and isoflavone content were positive correlated. It was deduced that elicitors stimulated plant secondary metabolites production associated with changes in cell structure.
Luo
Jian Ping
JP
School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009. jianpingluo@sohu.com
Xia
Ning
N
Shen
Guo Dong
GD
D000085:Acetates / Q000494:pharmacology*; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D007529:Isoflavones / Q000378:metabolism*; D029901:Maackia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000494:pharmacology*; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D020156:Salicylic Acid / Q000494:pharmacology*
D000085:Acetates; D003517:Cyclopentanes; D007529:Isoflavones; D054883:Oxylipins; D010937:Plant Growth Regulators; D009569:Nitric Oxide; C072239:methyl jasmonate; D020156:Salicylic Acid