title: pEGFP-T, a novel T-vector for the direct, unidirectional cloning and analysis of PCR-amplified promoters.
abstract: A novel T-vector, designated as pEGFP-T, was constructed which could be used for direct and unidirectional cloning and analysis of promoters. The method involves minimizing the non-recombinant background of the T-vector when used to clone PCR products, and maintaining only the forward orientation of the PCR products prior to transformation. The usefulness of this vector is demonstrated in cloning and analyzing promoters.
authors.affiliation: Key Research Lab of Hormone and Development Affiliated to the Public Health Ministry, Institute of Endocrinology, Tianjin Medical University, Tianjin, PR China. zhe0412@yahoo.com.cn.
authors.forename: Baoli
authors.initials: B
authors.lastname: Wang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Zheng
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Jinxing
authors.initials: J
authors.lastname: Quan
authors.forename: Hui
authors.initials: H
authors.lastname: Liang
authors.forename: Shanyi
authors.initials: S
authors.lastname: Guo
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Jingyu
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D049452:Green Fluorescent Proteins