Evolution of a Saccharomyces cerevisiae metabolic pathway in Escherichia coli.
The Saccharomyces cerevisiae glycerol pathway (GPD1 and GPP2) was evolved in vivo in Escherichia coli. The central metabolism of E. coli was engineered to link glucose consumption and glycerol production. The engineered strain was evolved in a chemostat culture and a high glycerol producer was rapidly obtained. The evolution of the strain was associated to a deletion between GPD1 and GPP2, resulting in the production of a fusion protein with both glycerol-3-P dehydrogenase and glycerol-3-P phosphatase activities. The higher efficiency of the fusion protein was due to partial glycerol-3-P channeling between the two active sites. The evolved strain produces glycerol from glucose at high yield, concentration and productivity.
Meynial Salles
Isabelle
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Laboratoire de Biotechnologie-Bioprocédés, UMR-INSA/CNRS 5504, UMR INSA/INRA 792, 135 avenue de Rangueil, 31077 Toulouse cedex 4, France.
Forchhammer
Nynne
N
Croux
Christian
C
Girbal
Laurence
L
Soucaille
Philippe
P
D000222:Adaptation, Physiological / Q000235:genetics; D005075:Biological Evolution; D004926:Escherichia coli / Q000235:genetics*; D019143:Evolution, Molecular; D005818:Genetic Engineering / Q000379:methods; D014644:Genetic Variation / Q000235:genetics; D005990:Glycerol / Q000378:metabolism*; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+) / Q000235:genetics*; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics*
D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D010744:Phosphoric Monoester Hydrolases; C040842:glycerol-1-phosphatase; D005990:Glycerol