The association of single nucleotide polymorphisms in the MMP-9 genes with susceptibility to acute primary angle closure glaucoma in Taiwanese patients.
OBJECTIVE
To study the relationships between single nucleotide polymorphisms (SNPs) of extracellular matrix, matrix metalloproteases (MMPs), tissue inhibitors of MMPs, and other glaucoma-associated genes and acute primary angle closure glaucoma (PACG).


METHODS
We extracted DNA samples from 78 adult patients with acute PACG and 86 control subjects to study the relationships between these specific genes and acute PACG. Genotyping was performed for 35 genes by the GenomeLab SNPstream genotyping system after PCR amplification of chromosomal DNA. The association between these genetic polymorphisms and risk of primary PACG was estimated by chi2 and logistic regression.


RESULTS
The genotyping success rate was 99%. Genotyping for the MMP9 site (rs2664538) was significantly different between the two groups (p=0.000001) and the odds ratio was 2.586 (95% CI: 1.715-3.898, p<0.00001). However, there were no associations of SNPs to other genes in patients with acute PACG.


CONCLUSIONS
Our results reveal that SNP rs2664538, which is located at the MMP9 gene, is likely to be associated with acute PACG.
Wang
I-Jong
IJ
Department of Ophthalmology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Chiang
Ting-Hsuan
TH
Shih
Yung-Feng
YF
Lu
Shao-Chun
SC
Lin
Luke Long-Kuang
LL
Shieh
Jui-Wen
JW
Wang
Tsing-Hong
TH
Samples
John R
JR
Hung
Por-Tying
PT
D000208:Acute Disease; D000368:Aged; D044466:Asian People / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015812:Glaucoma, Angle-Closure / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D016015:Logistic Models; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D013624:Taiwan
D020780:Matrix Metalloproteinase 9