title: Characterization of an early gene (wsv477) from shrimp white spot syndrome virus (WSSV).
abstract: White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. The viral early genes play a key role in DNA replication and virus proliferation. In this study, a WSSV gene (wsv477) encoding 208 amino acid peptides was characterized as an early gene. The temporal analysis showed that the wsv477 gene was first transcribed at 4 h post-infection, suggesting that it was an early gene. The wsv477 gene was expressed in Escherichia coli and purified. Subsequently the specific antibody was raised using the purified fusion protein (GST-WSV477). Western blot revealed that the wsv477 gene was expressed at 6 h post-infection in vivo. As indicated by GTP-binding assay, the WSV477 protein had GTP-binding activity.
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, Xiamen, 361005, P. R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Han
authors.forename: Jianyang
authors.initials: J
authors.lastname: Xu
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004267:DNA Viruses / Q000235:genetics / Q000378:metabolism*; D033364:Decapoda / Q000821:virology*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D046848:White spot syndrome virus 1 / Q000235:genetics*
chemical_list: D014764:Viral Proteins