title: Analysis of the soluble ATP-binding proteome of plant mitochondria identifies new proteins and nucleotide triphosphate interactions within the matrix.
abstract: The interactions of ATP inside plant mitochondria were investigated by identifying the soluble nucleotide binding proteome captured using immobilized ATP. Selected proteins were separated by 1D SDS-PAGE and 2D IEF-SDS-PAGE and identified by ESI-Q-TOF MS/MS. A range of highly enriched proteins were identified from the mitochondrial proteome, including 14-3-3 proteins and RNA binding proteins, as well as proteins known to contain nucleotide binding domains and/or to be inhibited or stimulated by ATP.
authors.affiliation: ARC Centre of Excellence in Plant Energy Biology and School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, M316, Crawley 6009, WA, Australia.
authors.forename: Jun
authors.initials: J
authors.lastname: Ito
authors.forename: Joshua L
authors.initials: JL
authors.lastname: Heazlewood
authors.forename: A Harvey
authors.initials: AH
authors.lastname: Millar
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000032:analysis / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis / Q000378:metabolism; D017360:Arabidopsis / Q000737:chemistry*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D013058:Mass Spectrometry; D008928:Mitochondria / Q000737:chemistry*; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics
chemical_list: D018528:ATP-Binding Cassette Transporters; D048868:Adaptor Proteins, Signal Transducing; D024101:Mitochondrial Proteins; D010940:Plant Proteins