Dialysis membrane-induced oxidative stress: role of heme oxygenase-1.
Dialysis membranes have been reported to induce monocyte apoptosis. We studied the role of oxidative stress in the induction of dialysis membrane-induced monocyte apoptosis. Superoxide dismutase, a superoxide scavenger, prevented dialysis membrane-induced monocyte apoptosis. Similarly, other antioxidants also inhibited dialysis membrane- induced apoptosis. In addition, the interaction of dialysis membranes with monocytes was associated with the generation of molecules leading to oxidative stress such as superoxide and TBARS. Interestingly, pre-induction of heme oxygenase (HO)-1 by hemin prevented dialysis membrane-induced monocyte apoptosis, whereas inhibition of HO-1 activity (treatment with tin protoporphyrin, SN-P) enhanced dialysis membrane-induced monocyte apoptosis. We suggest that oxidative injury associated with dialysis membrane and monocyte interaction plays a role in monocyte injury. Pre-induction of HO-1 may attenuate dialysis membrane-induced monocyte apoptosis.
Bhaskaran
Madhu
M
Division of Kidney Diseases and Hypertension, North Shore University Hospital, Manhasset and Long Island Jewish Medical Center, New Hyde Park, NY, USA.
Radhakrishnan
Neetu
N
Patni
Hitesh
H
Singh
Priyanka
P
Chaudhary
Ahmad N
AN
Singhal
Pravin C
PC
D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis; D002482:Cellulose / Q000031:analogs & derivatives / Q000494:pharmacology; D053938:DNA Fragmentation; D051547:Heme Oxygenase-1 / Q000502:physiology*; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D015227:Lipid Peroxidation; D008567:Membranes, Artificial; D009000:Monocytes / Q000378:metabolism*; D018384:Oxidative Stress; D006435:Renal Dialysis / Q000009:adverse effects*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D008567:Membranes, Artificial; D013481:Superoxides; C005062:acetylcellulose; D006427:Hemin; D002482:Cellulose; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase