DNA isolation from goat blood using different brands of household detergents and its downstream application.
Rapid isolation of DNA from goat blood using different brands of detergents available in Indian market, is reported. The integrity and efficiency of these DNA preparations were compared with genomic DNA isolated by a standard kit (Flexi gene DNA kit), using amplification of exon 2 of CYP19 (aromatase) gene. The similar and significant amplification of this gene was obtained using genomic DNA isolated by kit and various detergents. However, among the detergents used, the Rin and Ezee were found to be the best to get DNA of high purity comparable to that obtained by kit.
Kumar
O Suneel
OS
Molecular Endocrinology Lab, Animal Biochemistry Division, National Dairy Research Institute, Karnal 132 001, India.
Sharma
M K
MK
Singh
Dheer
D
D000818:Animals; D001141:Aromatase / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification; D003902:Detergents; D004587:Electrophoresis, Agar Gel; D006041:Goats / Q000097:blood* / Q000235:genetics; D016133:Polymerase Chain Reaction
D003902:Detergents; D004247:DNA; D001141:Aromatase