title: Detection of Coxiella burnetii in market chicken eggs and mayonnaise.
abstract: We tried to detect C. burnetii in market chicken eggs and mayonnaise by nested PCR assay. The PCR target was the com 1 gene of C. burnetii. The positive rate for egg and mayonnaise samples was 4.2% and 17.6%, respectively. Direct sequence of some of the positive egg samples shows mutations whereas no mutation was found in the positive mayonnaise samples. The number of molecules of the Q fever agent is estimated at 10(4) to 10(6) per egg, according to our quantitative PCR test.
authors.affiliation: Institute for Preventive Medicine against Zoonosis (IPMZ), 4569-1, Komoridani, Oyabe City, Toyama Prefecture, 932-0133 Japan. info@jinjyuken.gr.jp
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Tatsumi
authors.forename: Andreas
authors.initials: A
authors.lastname: Baumgartner
authors.forename: Ying
authors.initials: Y
authors.lastname: Qiao
authors.forename: Ikkyu
authors.initials: I
authors.lastname: Yamamoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D016997:Coxiella burnetii / Q000235:genetics / Q000302:isolation & purification*; D004531:Eggs / Q000382:microbiology*; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D016133:Polymerase Chain Reaction