Mapping candidate genes for Drosophila melanogaster resistance to the parasitoid wasp Leptopilina boulardi.
Drosophila melanogaster resistance against the parasitoid wasp Leptopilina boulardi is under the control of a single gene (Rlb), with two alleles, the resistant one being dominant. Using strains bearing deletions, we previously demonstrated that the 55E2-E6; 55F3 region on chromosome 2R is involved in the resistance phenomenon. In this paper, we first restricted the Rlb containing region by mapping at the molecular level the breakpoints of the Df(2R)Pc66, Df(2R)P34 and Df(2R)Pc4 deficiencies, using both chromosomal in situ hybridization and Southern analyses. The resistance gene was localized in a 100 kb fragment, predicted to contain about 10 different genes. Male recombination genetic experiments were then performed, leading to identification of two possible candidates for the Rlb gene. Potential involvement of one of this genes, edl/mae, is discussed.
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Institut de Recherche sur la Biologie de l'Insecte - UMR CNRS 6035, Université François Rabelais, Parc Grandmont, 37200 Tours, France.
Espagne
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Lemeunier
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Pascual
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Carton
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Periquet
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Poirie
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D000818:Animals; D002874:Chromosome Mapping; D003360:Cosmids / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000469:parasitology*; D017344:Genes, Insect; D005809:Genes, Regulator; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D007814:Larva / Q000235:genetics / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008957:Models, Genetic; D011995:Recombination, Genetic; D014863:Wasps
D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C092343:Rlb1 protein, Drosophila; C428945:edl protein, Drosophila