title: Isolation of Anaplasma phagocytophilum strain Ap-variant 1 in a tick-derived cell line.
abstract: Ten isolates of the Ap-Variant 1 strain of Anaplasma phagocytophilum were made in the Ixodes scapularis (I. scapularis)-derived cell line, ISE6. Two isolates were obtained from laboratory-infected goats and eight isolates were obtained from field-collected I. scapularis ticks. Each isolate showed 16S rRNA sequences identical to those as previously described for the Ap-Variant 1 strain. These are the first tissue culture isolates of the Ap-Variant 1 strain and will allow for further characterization of the biological and antigenic properties of this strain.
authors.affiliation: Centers for Disease Control and Prevention, 1600 Clifton Rd., MS G-13, Atlanta, GA 30333, USA. rfm2@cdc.gov
authors.forename: Robert F
authors.initials: RF
authors.lastname: Massung
authors.forename: Michael L
authors.initials: ML
authors.lastname: Levin
authors.forename: Ulrike G
authors.initials: UG
authors.lastname: Munderloh
authors.forename: David J
authors.initials: DJ
authors.lastname: Silverman
authors.forename: Meghan J
authors.initials: MJ
authors.lastname: Lynch
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Kurtti
mesh_terms: D041081:Anaplasma phagocytophilum / Q000235:genetics* / Q000302:isolation & purification*; D000818:Animals; D002460:Cell Line; D016873:Ehrlichiosis / Q000382:microbiology / Q000473:pathology; D014644:Genetic Variation; D006041:Goats / Q000382:microbiology; D006801:Humans; D013987:Ticks / Q000382:microbiology*