Detection of Coxiella burnetii in market chicken eggs and mayonnaise.
We tried to detect C. burnetii in market chicken eggs and mayonnaise by nested PCR assay. The PCR target was the com 1 gene of C. burnetii. The positive rate for egg and mayonnaise samples was 4.2% and 17.6%, respectively. Direct sequence of some of the positive egg samples shows mutations whereas no mutation was found in the positive mayonnaise samples. The number of molecules of the Q fever agent is estimated at 10(4) to 10(6) per egg, according to our quantitative PCR test.
Tatsumi
Noriyuki
N
Institute for Preventive Medicine against Zoonosis (IPMZ), 4569-1, Komoridani, Oyabe City, Toyama Prefecture, 932-0133 Japan. info@jinjyuken.gr.jp
Baumgartner
Andreas
A
Qiao
Ying
Y
Yamamoto
Ikkyu
I
Yamaguchi
Kazuo
K
D016997:Coxiella burnetii / Q000235:genetics / Q000302:isolation & purification*; D004531:Eggs / Q000382:microbiology*; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D016133:Polymerase Chain Reaction