title: Detection of anti-heat shock protein 90 beta (Hsp90beta) antibodies in cerebrospinal fluid.
abstract: Antibodies against heat shock protein 90 beta (Hsp90beta) recognize the antigen on the cell surface of the oligodendrocyte precursor cells and cause a decrease of oligodendrocyte population in cell cultures. These antibodies have been found in patients with multiple sclerosis (MS). This report describes an original and sensitive method to detect anti-Hsp90beta antibodies in cerebrospinal fluid (CSF) using a western blot procedure. We have developed the method for autoantibody detection using Hsp90beta from cell membrane fraction instead of commercial Hsp90beta as antigen. The presence of anti-Hsp90beta antibodies in CSF of MS patients may play a pathogenic role in MS, and a large-scale study is needed to establish a possible diagnostic value of these antibodies in MS patients.
authors.affiliation: Department of Investigation, Hospital Ramón y Cajal, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Cid
authors.forename: M
authors.initials: M
authors.lastname: García-Villanueva
authors.forename: M
authors.initials: M
authors.lastname: Salinas
authors.forename: A
authors.initials: A
authors.lastname: Alcázar
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000134:cerebrospinal fluid*; D015153:Blotting, Western / Q000379:methods; D002457:Cell Extracts / Q000737:chemistry / Q000276:immunology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000276:immunology; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D018841:HSP90 Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D047468:Immunoprecipitation; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000276:immunology; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid / Q000276:immunology; D009836:Oligodendroglia / Q000737:chemistry / Q000276:immunology; D051381:Rats
chemical_list: D001323:Autoantibodies; D002457:Cell Extracts; D018841:HSP90 Heat-Shock Proteins