Microchimerism evaluation in recipients of living-related or unrelated deceased allograft renal transplants.
The presence of microchimerism in the peripheral blood of solid organ graft recipients has been associated with long-term solid organ acceptance, immunologic tolerance, and less aggressive immunosuppressive therapy. Molecular biology assays are among the most sensitive methods to detect microchimerism, primarily to evaluate Y chromosome sequences in females as indirect evidence of circulating male nucleated donor cells. We screened for the presence of the SRY sequence region in peripheral blood of 13 female recipients of male kidney grafts: 5 living-related and 8 deceased grafts. Only patients who received grafts from related living donors exhibited microchimerism. Five of 13 patients studied exhibited better graft outcomes, including the 4 who were positive for the SRY sequences.
Crispim
J C d O
JC
Department of Biochemistry and Immunology, FMRP-USP Brazil, Ribeiro Preto, Sao Paulo, Brazil. janacrispim@rpm.fmrp.usp.br
Wastowski
I J
IJ
Faggioni
L P C
LP
Costa
R
R
Saber
L
L
Donadi
E A
EA
D000328:Adult; D001483:Base Sequence; D002102:Cadaver; D041322:Chromosomes, Human, Y; D017931:DNA Primers; D005190:Family; D005260:Female; D006650:Histocompatibility Testing; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology / Q000502:physiology*; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D014019:Tissue Donors; D018183:Transplantation Chimera
D017931:DNA Primers