In vitro induction of differentiation by retinoic acid in an immortalized olfactory neuronal cell line.
In this study, we used a neuronal cell line generated by transfection of rat olfactory epithelium with immortalizing recombinant oncogene E1A of adenovirus-2. The resulting 13.S.1.24 line of transformed cells expressed an antigenic phenotype of olfactory neuronal progenitors. Time-dependency assessments over 1 week of treatment indicated that apoptosis and differentiation induced by retinoic acid (RA) were concomitant. Indeed, RA altered the cell proliferation rate, but it also stimulated differentiation of surviving 13.S.1.24 cells into bipolar olfactory marker protein-immunoreactive neurons. To characterize the nature of the cells we used immunocytochemistry, optical imaging, scanning electron microscopy and atomic force microscopy.
Lakard
Sophie
S
Laboratory of Neurosciences, University of Franche-Comté, Besançon, France. sophie.lakard@univ-fcomte.fr
Lesniewska
Eric
E
Michel
Germaine
G
Lakard
Boris
B
Morrand-Villeneuve
Nadege
N
Versaux-Botteri
Claudine
C
D000262:Adenylyl Cyclases / Q000032:analysis; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002461:Cell Line, Transformed; D049109:Cell Proliferation / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D009419:Nerve Tissue Proteins / Q000032:analysis; D064231:Nestin; D009474:Neurons / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D009831:Olfactory Mucosa / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology*; D014404:Tubulin / Q000032:analysis
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D014404:Tubulin; D014212:Tretinoin; D000262:Adenylyl Cyclases