title: Yeast flavohemoglobin protects against nitrosative stress and controls ferric reductase activity.
abstract: The role of Saccharomyces cerevisiae flavohemoglobin (Yhb1) is controversial and far from understood. This study compares the effects of nitrosative and oxidative challenge on the yeast mutant lacking the YHB1 gene. Growth of the mutant was impaired by nitrosoglutathione and peroxynitrite, whereas increased sensitivity to reactive oxygen species was not observed. Increased levels of intracellular NO(*) after incubation with NO(*) donors were found in the mutants cells as compared to the wild-type cells. Deletion of the YHB1 gene was found to augment the reduction of Fe(3+) by yeast cells which suggests that flavohemoglobin participates in regulation of the activity of plasma membrane ferric reductase(s).
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, Rzeszow, Poland. alewinska@o2.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Lewinska
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
mesh_terms: D002455:Cell Division / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D003676:Deferoxamine / Q000494:pharmacology; D049308:Dioxygenases; D004305:Dose-Response Relationship, Drug; D038181:FMN Reductase / Q000378:metabolism*; D005292:Ferricyanides / Q000378:metabolism; D017353:Gene Deletion; D006420:Hemeproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D009569:Nitric Oxide / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009603:Nitroso Compounds / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D010396:Penicillamine / Q000031:analogs & derivatives / Q000494:pharmacology; D030421:Peroxynitrous Acid / Q000494:pharmacology; D026422:S-Nitrosoglutathione / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013997:Time Factors
chemical_list: D005292:Ferricyanides; D006420:Hemeproteins; D020030:Nitric Oxide Donors; D009603:Nitroso Compounds; C110881:S-nitro-N-acetylpenicillamine; D029701:Saccharomyces cerevisiae Proteins; C007931:hexacyanoferrate III; C094210:2,2'-(hydroxynitrosohydrazono)bis-ethanamine; D030421:Peroxynitrous Acid; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D049308:Dioxygenases; C101796:YHB1 protein, S cerevisiae; D038181:FMN Reductase; C023903:ferric citrate iron reductase; D010396:Penicillamine; D003676:Deferoxamine