title: Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library.
abstract: The utility of blue fluorescent protein (BFP) has been limited by its low quantum yield and rapid photobleaching. A library targeting residues neighboring the chromophore yielded a variant with enhanced quantum yield (0.55 versus 0.34), reduced pH sensitivity and a 40-fold increase in photobleaching half-life. This BFP, named Azurite, is well expressed in bacterial and mammalian cells and extends the palette of fluorescent proteins that can be used for imaging.
authors.affiliation: Department of Chemical Engineering, University of California, Santa Barbara, California 93106, USA.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Mena
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Treynor
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Mayo
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Daugherty
mesh_terms: D005434:Flow Cytometry; D015723:Gene Library; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D038761:Photobleaching; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D008164:Luminescent Proteins; D011994:Recombinant Proteins