Dietary fructooligosaccharides alter the cultivable faecal population of rats but do not stimulate the growth of intestinal bifidobacteria.
The effect of fructans on the cultivable faecal community of Bio Breeding rats fed diets containing 5% (m/v) food-grade fructooligosaccharide (FOS) was investigated. Culturing of faecal material using chicory inulin as the sole carbohydrate source revealed the presence of a greater diversity of inulin-utilizing bacterial species in FOS-fed rats as compared with the control rats, although both contained species which effectively utilized inulin. The majority of cultivable inulin-utilizing species fell within the Clostridium coccoides group and Clostridium leptum subgroup, some of which were related to previously cultured butyrate-producing bacteria from the intestines of various animals. The impact of FOS on the growth of the indigenous bifidobacteria community and three inulin-utilizing isolates was assessed using real-time polymerase chain reaction. While dietary FOS was found to stimulate the growth of all three inulin-utilizing isolates, no growth stimulation of the indigenous bifidobacteria community occurred over the duration of the feeding trial.
Gourgue-Jeannot
C
C
Bureau of Nutritional Research, Food Directorate, Health Products and Foods Branch, Health Canada, Banting Research Centre, Ottawa, ON, Canada.
Kalmokoff
M L
ML
Kheradpir
E
E
Kwan
J
J
Lampi
B J
BJ
McAllister
M
M
Brooks
S P J
SP
D000818:Animals; D001644:Bifidobacterium / Q000254:growth & development* / Q000302:isolation & purification; D018651:Cichorium intybus / Q000378:metabolism; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D004032:Diet; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004063:Digestion; D005243:Feces / Q000382:microbiology*; D006094:Gram-Positive Bacteria / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D007422:Intestines / Q000382:microbiology*; D007444:Inulin / Q000378:metabolism; D009844:Oligosaccharides / Q000008:administration & dosage* / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D011913:Rats, Inbred BB; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003470:Culture Media; D004040:Dietary Carbohydrates; D009844:Oligosaccharides; C116580:fructooligosaccharide; D007444:Inulin