title: Proteome mapping of the Trichoderma reesei 20S proteasome.
abstract: The 26S proteasome, a multicatalytic protease comprising the catalytic 20S core particle and the 19S regulatory particle has a crucial role in cellular protein quality control. We have used a chromatography-based approach to purify and map the protein content of the 20S core particle from the industrially-exploited filamentous fungus Trichoderma reesei. There are no previous reports on the isolation or proteomic mapping of the proteasome from any filamentous fungus. From the reference map, 13 of the 14 20S proteasome subunits and many related proteins that co-purified with the 20S proteasome have been identified. These include 78 kDa glucose-regulated protein (BIP) and several chaperones including heat shock proteins involved in the unfolded protein response (UPR). Some proteasome interacting proteins (PIPs) were also identified on the proteome map and included 14-3-3-like protein, glyceraldehyde-3-phosphate dehydrogenase, transaldolase, actin, translation elongation factor, enolase, ATPase in the ER (CDC48), and eukaryotic initiation factor. We present here a master map for the 20S catalytic core to pave the way for future differential display studies addressing intracellular degradation of endogenous and foreign proteins in filamentous fungi.
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, 2109, Australia.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Grinyer
authors.forename: Liisa
authors.initials: L
authors.lastname: Kautto
authors.forename: Mathew
authors.initials: M
authors.lastname: Traini
authors.forename: Robert D
authors.initials: RD
authors.lastname: Willows
authors.forename: Junior
authors.initials: J
authors.lastname: Te'o
authors.forename: Peter
authors.initials: P
authors.lastname: Bergquist
authors.forename: Helena
authors.initials: H
authors.lastname: Nevalainen
mesh_terms: D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D020543:Proteome; D046988:Proteasome Endopeptidase Complex