title: Ultrahigh-resolution study of protein atomic displacement parameters at cryotemperatures obtained with a helium cryostat.
abstract: Two X-ray data sets for a complex of human aldose reductase (h-AR) with the inhibitor IDD 594 and the cofactor NADP(+) were collected from two different parts of the same crystal to a resolution of 0.81 A at 15 and 60 K using cold helium gas as cryogen. The contribution of temperature to the atomic B values was estimated by comparison of the independently refined models. It was found that although being slightly different for different kinds of atoms, the differences (deltaB) in the isotropic equivalents B of atomic displacement parameters (ADPs) were approximately constant (about 1.7 A(2)) for well ordered atoms as the temperature was increased from 15 to 60 K. The mean value of this difference varied according to the number of non-H atoms covalently bound to the parent atom. Atoms having a B value of higher than 8 A(2) at 15 K showed much larger deviations of deltaB from the average value, which might reflect partial occupancy of atomic sites. An analysis of the anisotropy of ADPs for individual atoms revealed an increase in the isotropy of ADPs with the increase of the temperature from 15 to 60 K. In a separate experiment, a 0.93 A resolution data set was collected from a different crystal of the same complex at 100 K using cold nitrogen as a cryogen. The effects of various errors on the atomic B values were estimated by comparison of the refined models and the temperature-dependent component was inferred. It was found that both decreasing the data redundancy and increasing the resolution cutoff led to an approximately constant increase in atomic B values for well ordered atoms.
authors.affiliation: Structural Biology Center, Biosciences Division, Argonne National Laboratory, Argonne, Illinois 60439, USA.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Petrova
authors.forename: Stephan
authors.initials: S
authors.lastname: Ginell
authors.forename: Andre
authors.initials: A
authors.lastname: Mitschler
authors.forename: Isabelle
authors.initials: I
authors.lastname: Hazemann
authors.forename: Thomas
authors.initials: T
authors.lastname: Schneider
authors.forename: Alexandra
authors.initials: A
authors.lastname: Cousido
authors.forename: Vladimir Y
authors.initials: VY
authors.lastname: Lunin
authors.forename: Andrzej
authors.initials: A
authors.lastname: Joachimiak
authors.forename: Alberto
authors.initials: A
authors.lastname: Podjarny
mesh_terms: D000449:Aldehyde Reductase / Q000737:chemistry*; D016880:Anisotropy; D002244:Carbon; D003080:Cold Temperature; D018360:Crystallography, X-Ray; D005615:Freezing; D006371:Helium; D006801:Humans; D008958:Models, Molecular; D010100:Oxygen; D011487:Protein Conformation
chemical_list: D006371:Helium; D002244:Carbon; D000449:Aldehyde Reductase; D010100:Oxygen