Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.).
A robust Agrobacterium-mediated transformation procedure was developed for Rigel, a commercial cultivar of evening primrose, and used to deliver a cDNA encoding a Delta(6)-desaturase from borage under the control of a cauliflower mosaic virus (CaMV) 35S promoter. Analysis of the transformed plants demonstrated an altered profile of polyunsaturated fatty acids, with an increase in gamma-linolenic acid and octadecatetraenoic acid in leaf tissues when compared with control lines.
de Gyves
Emilio Mendoza
EM
CPI Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK.
Sparks
Caroline A
CA
Sayanova
Olga
O
Lazzeri
Paul
P
Napier
Johnathan A
JA
Jones
Huw D
HD