title: [Liquid chromatography-tandem electrospray ionization ion trap mass spectrometric assay for the metabolites of jatrorrhizine in rat urine].
abstract: OBJECTIVE
  To identify the main metabolites of jatrorrhizine in rat urine.
  
  
  METHODS
  The rat urine samples were collected 0 - 72 h after ig 12 mg x kg(-1) jatrorrhizine, then the samples were purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by combining liquid chromatography and tandem electrospray ionization ion trap mass spectrometry (LC-ESI/ITMS(n)). Identification and structural elucidation of the metabolites were performed by comparing the changes in molecular masses, retention-times and full scan MS(n) spectra with those of the parent drug.
  
  
  RESULTS
  At least seven phase I metabolites (such as de-methyl, de-hydrogen and hydroxyl metabolites) and eleven phase II metabolites (such as glucuronide conjugates and methyl-conjugates) were identified in rat urine.
  
  
  CONCLUSIONS
  The developed LC-ESI/ITMS(n) method is not only simple and rapid but also sensitive and specific for the identification of metabolites of jatrorrhizine in rat urine.
authors.affiliation: Hubei Province Key Laboratory of Bio-Technology for Traditional Chinese Medicine, Hubei University, Wuhan 430062, China.
authors.forename: Feng-mei
authors.initials: FM
authors.lastname: Han
authors.forename: Ming-ming
authors.initials: MM
authors.lastname: Zhu
authors.forename: Huai-xia
authors.initials: HX
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D001599:Berberine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D039323:Coptis / Q000737:chemistry; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: C055785:jatrorrhizine; D001599:Berberine