Preparation of validoxylamine A by biotransformation of validamycin A using resting cells of a recombinant Escherichia coli.
A gene encoding beta-glucosidase was cloned and over-expressed in Escherichia coli. Validamycin A was then biotransformed into validoxylamine A by using the resting recombinant cells. The biotransformation yield reached 92% when the reaction was performed at 37 degrees C for 1 h in the presence of 100 ml sodium phosphate buffer (0.1 M, pH 7.0), 32 mM validamycin A and 0.71 mg dry cell w/ml.
Yin
Yu
Y
Department of Microbiology, Nanjing Agricultural University, Nanjing, PR China. hccbyinyu@hotmail.com
Huang
Weiyi
W
Chen
Daijie
D
D001711:Biotransformation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007294:Inositol / Q000031:analogs & derivatives* / Q000378:metabolism; D013696:Temperature; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism
C052534:validoxylamine A; D007294:Inositol; C003749:validamycins; D001617:beta-Glucosidase