title: Cytoskeletal structures, ultrastructural characteristics and the capsule of the basidiomycetous yeast Cryptococcus laurentii.
abstract: The cytoskeleton, capsule and cell ultrastructure were studied during the cell cycle of Cryptococcus laurentii. In an encapsulated strain, cytoplasmic microtubules and a mitotic spindle were detected. Mitosis was preceded by migration of the nucleus into the bud. F-actin failed to be visualised by rhodamine-phalloidin (RhPh) in encapsulated cells and therefore an acapsular strain was used. The following actin structures were found: actin dots, actin cables and cytokinetic ring. Ultrastructural studies showed the presence of a nucleus in the bud before mitosis. A collar-shaped structure was seen at the base of bud emergence. A lamellar cell wall and a rough outer surface of the cells were detected. Cytoskeletal structures found in C. laurentii are similar to those in Cryptococcus neoformans, which is a serious human pathogen.
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Tomesova 12, Brno, 602 00, Czech Republic. mdavid@med.muni.cz
authors.forename: Marek
authors.initials: M
authors.lastname: David
authors.forename: Miroslav
authors.initials: M
authors.lastname: Gabriel
authors.forename: Marie
authors.initials: M
authors.lastname: Kopecká
mesh_terms: D000199:Actins / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002473:Cell Wall / Q000378:metabolism; D003454:Cryptococcus / Q000378:metabolism* / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D008954:Models, Biological; D010590:Phalloidine / Q000031:analogs & derivatives / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry
chemical_list: D000199:Actins; D012235:Rhodamines; C504731:rhodamine-phalloidin; D010590:Phalloidine