Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library.
The utility of blue fluorescent protein (BFP) has been limited by its low quantum yield and rapid photobleaching. A library targeting residues neighboring the chromophore yielded a variant with enhanced quantum yield (0.55 versus 0.34), reduced pH sensitivity and a 40-fold increase in photobleaching half-life. This BFP, named Azurite, is well expressed in bacterial and mammalian cells and extends the palette of fluorescent proteins that can be used for imaging.
Mena
Marco A
MA
Department of Chemical Engineering, University of California, Santa Barbara, California 93106, USA.
Treynor
Thomas P
TP
Mayo
Stephen L
SL
Daugherty
Patrick S
PS
D005434:Flow Cytometry; D015723:Gene Library; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D038761:Photobleaching; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics
D008164:Luminescent Proteins; D011994:Recombinant Proteins