[Tissue culture and high-frequency plant regeneration of buckwheat (Fagopyrum esculentum Moench)].
Different explants and compounding proportions of different hormones were comparatively studied in tissue culture of Fagopyrum esculentum Moench and thereafter an efficient plant-regeneration system was established by in vitro F. esculentum Moench culture. On MS medium containing 2.0 mg/L 2,4-D and 1.0 mg/L 6-BA, 89.6% cotyledon segments could be induced to produce calli; however, on MS medium containing 2.0 mg/L 2,4-D and 1.0-2.0 mg/L 6-BA, the induction rate of hypocotyl segments could reach as high as 100%. On MS medium containing 2.0 mg/L 6-BA, 0.1 mg/L IAA and 1 mg/L KT, adventitious buds could be regenerated indirectly from calli or directly from explants, the differentiation rates of cotyledon-derived calli and hypocotyl-derived calli were 42.5% and 73.6% respectively; the calli coming from hypocotyl segments differentiated evidently at a higher rate than the calli originated from cotyledon segments. Well-grown adventitious buds were inoculated on 1/2 MS medium containing 1.0 mg/L IBA and 0.5 mg/L NAA, 100% rooting frequency was obtained. Plantlets grew well and appeared normal with no mortality after being transplanted to soil. Moreover, the survival rate of plantlets reached 91.6%.
Chen
Li Hong
LH
Shaanxi Provincial Key Laboratory of Biotechnology, Northwest University, Xi'an 710069.
Xu
Zi Qin
ZQ
D019612:Fagopyrum / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*; D046509:Tissue Culture Techniques / Q000379:methods