Screening of peptide-displaying phage libraries to identify short peptides mimicking carbohydrates.
Peptide-displaying phage technology has numerous applications. Using a specificity-defined monoclonal anti-carbohydrate antibody, we can identify a series of short peptides that mimic the binding specificity of a specific carbohydrate. This chapter introduces pioneering work applying phage display technology to the glycobiology field, presents a step-by-step protocol for phage library screening, and provides useful hints for evaluating results including false positives, all of which should contribute to successful cloning. Thus, biopanning using a monoclonal antibody as the target is described in detail. Because peptides are useful alternatives to carbohydrate ligands, their potential use as structural or functional mimics of carbohydrate-binding proteins is discussed.
Fukuda
Michiko N
MN
Glycobiology Program, Cancer Research Center, The Burnham Institute, La Jolla, CA, USA.
D000911:Antibodies, Monoclonal / Q000737:chemistry; D001435:Bacteriophages; D001483:Base Sequence; D001666:Binding Sites, Antibody; D002241:Carbohydrates / Q000737:chemistry*; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D019151:Peptide Library; D010455:Peptides