A single-nucleotide synonymous mutation in the gag gene controlling human immunodeficiency virus type 1 virion production.
Viral factors as well as host ones play major roles in the disease progression of human immunodeficiency virus type 1 (HIV-1) infection. We have examined cytotoxic T-lymphocyte activity and HIV-1 DNA PCR results of 312 high-risk seronegative drug users in northern Thailand and identified four seronegative cases positive for both assays. Furthermore, we have identified a synonymous mutation in nucleotide position 75 of the gag p17 gene (A426G) of HIV-1 that belongs to the CRF01_AE virus circulating in Thailand. The replication-competent HIV-1 clone containing the A426G mutation demonstrated a dramatic reduction of virion production and perturbation of viral morphogenesis without affecting viral protein synthesis in cells.
Hamano
Takaichi
T
National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Matsuo
Kazuhiro
K
Hibi
Yurina
Y
Victoriano
Ann Florence B
AF
Takahashi
Naoko
N
Mabuchi
Yosio
Y
Soji
Tsuyoshi
T
Irie
Shinji
S
Sawanpanyalert
Pathom
P
Yanai
Hideki
H
Hara
Takashi
T
Yamazaki
Shudo
S
Yamamoto
Naoki
N
Okamoto
Takashi
T
D004279:DNA, Viral / Q000235:genetics; D015750:Genes, gag / Q000235:genetics / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D009154:Mutation; D009711:Nucleotides; D014771:Virion / Q000502:physiology*
D004279:DNA, Viral; D009711:Nucleotides