title: Molecular characterization of Czech and Chinese leek yellow stripe virus isolates from garlic.
abstract: Using an RT-PCR specific for nuclear inclusion b (NIb) and coat protein (CP) genes of Leek yellow stripe virus (LYSV) we detected two Czech (LYSV-5CZ and LYSV-22CZ) and one Chinese (LYSV-16) isolate of LYSV in garlic plants. The RT-PCR products were cloned and sequenced. Phylogenetic analysis based on deduced amino acid sequence of NIb-CP region placed the Czech isolates in the group I and the Chinese isolate in the group II of LYSV.
authors.affiliation: Faculty of Sciences, Palack ý University, 783 71 Olomouc, Czech Republic. milan.navratil@upol.cz
authors.forename: M
authors.initials: M
authors.lastname: Navrátil
authors.forename: D
authors.initials: D
authors.lastname: Safárová
authors.forename: E
authors.initials: E
authors.lastname: Tkadlecová
authors.forename: J
authors.initials: J
authors.lastname: Klukácková
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002681:China; D003001:Cloning, Molecular; D018153:Czech Republic; D012321:DNA-Directed RNA Polymerases; D005737:Garlic / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D014764:Viral Proteins; C061396:nuclear inclusion protein b, mosaic viruses; D012321:DNA-Directed RNA Polymerases