title: [Determination in vitro of rat plasma protein binding rate of sinomenine by using microdialysis method].
abstract: OBJECTIVE
  To determine in vitro the rat plasma protein binding rate by using microdialysis method.
  
  
  METHODS
  The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method. The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate. The x-intercept of regression equation was the free drug concentration (Cf). The plasma protein binding rate was calculated by using the following equation: f = ( C0 - Cf)/C0.
  
  
  RESULTS
  The binding rate was kept relatively stable in the studied concentration range.
  
  
  CONCLUSIONS
  It is feasible that the plasma protein binding rate can be determined by using microdialysis method.
authors.affiliation: Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China. fredzhangfeng@163.com
authors.forename: Ying-feng
authors.initials: YF
authors.lastname: Zhang
authors.forename: Li-ling
authors.initials: LL
authors.lastname: Zhou
authors.forename: Rui
authors.initials: R
authors.lastname: Li
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D017551:Microdialysis / Q000379:methods*; D009019:Morphinans / Q000302:isolation & purification / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis; D032841:Sinomenium / Q000737:chemistry
chemical_list: D001798:Blood Proteins; D009019:Morphinans; C009271:sinomenine