Cadmium effects on populations of root nuclei in two pea genotypes inoculated or not with the arbuscular mycorrhizal fungus Glomus mosseae.
Plants possess a broad range of strategies to cope with cadmium (Cd) stress, including the arbuscular mycorrhizal (AM) symbiosis. In cell responses towards Cd, the contribution of changes in ploidy levels is still unclear. We used flow cytometry to investigate if nuclear ploidy changes are involved in response mechanisms toward Cd and to analyze the effect of the symbiotic status on populations of nuclei. The impact of Cd was investigated in roots of two pea (Pisum sativum L.) genotypes differing in their Cd-sensitivity (Cd-sensitive VIR4788 and Cd-tolerant VIR7128). In pea seedlings grown under hydropony, 25 and 250 microM Cd concentrations lead to an increase in 4 C together with a decrease in 2 C nuclei. The same genotypes, grown in soil/sand substrate, were inoculated or not with the AM fungus Glomus mosseae BEG12 and treated or not with Cd at transplanting (Cd1) or 2 weeks after (Cd2). The Cd2 increased the proportion of 6 and 8 C nuclei in the mycorrhizal VIR4788 and in the non-mycorrhizal VIR7128 genotypes. Thus, changes in ploidy levels reflect pea responses towards Cd, which are modulated by the symbiotic interaction. The Cd-induced increase in ploidy may account for changes in DNA transcription and/or translation.
Repetto
Ombretta
O
Department of Environmental and Life Science, University of Piemonte Orientale 'Amedeo Avogadro', Via Bellini 25G, 15100, Alessandria, Italy.|UMR 1088 INRA/CNRS5484/UB, PME (Plante-Microbe-Environnement) INRA-CMSE, Domaine d'Epoisses, BP 86510, 21065, Dijon Cedex, France.
Massa
Nadia
N
Department of Environmental and Life Science, University of Piemonte Orientale 'Amedeo Avogadro', Via Bellini 25G, 15100, Alessandria, Italy.
Gianinazzi-Pearson
Vivienne
V
UMR 1088 INRA/CNRS5484/UB, PME (Plante-Microbe-Environnement) INRA-CMSE, Domaine d'Epoisses, BP 86510, 21065, Dijon Cedex, France.
Dumas-Gaudot
Eliane
E
UMR 1088 INRA/CNRS5484/UB, PME (Plante-Microbe-Environnement) INRA-CMSE, Domaine d'Epoisses, BP 86510, 21065, Dijon Cedex, France. dumas@epoisses.inra.fr.
Berta
Graziella
G
Department of Environmental and Life Science, University of Piemonte Orientale 'Amedeo Avogadro', Via Bellini 25G, 15100, Alessandria, Italy.
D002104:Cadmium / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects*; D005434:Flow Cytometry; D005838:Genotype; D038821:Mycorrhizae / Q000502:physiology*; D018532:Pisum sativum / Q000187:drug effects* / Q000235:genetics / Q000382:microbiology; D011003:Ploidies
D002104:Cadmium