title: Quantification of seven arsenic compounds in seafood products by liquid chromatography/electrospray ionization-single quadrupole mass spectrometry (LC/ESI-MS).
abstract: A liquid chromatography/electrospray ionization-single quadrupole mass spectrometry (LC/ESI-MS) method was developed to quantify seven arsenic compounds: arsenate (As(V)), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine (AB), trimethylarsine oxide (TMAO), arsenocholine (AC) and tetramethylarsonium ion (TEMA), widely found in seafood. The arsenicals separated by anion- or cation-exchange LC were all readily identified under the optimized ESI-MS conditions. Linear calibration curves constructed by plotting the peak area counts of molecular ions against the arsenic concentrations were obtained for all seven arsenic compounds. The limits of quantification (S/N = 10) were 800, 600, 50, 10, 5, 5 and 5 ng ml-1 for As(V), MMA, DMA, AB, TMAO, AC and TEMA, respectively. The LC/ESI-MS method was found to be useful to quantify arsenic compounds in seafood by model experiments using the mid-gut gland and muscle of a shellfish (Buccinid whelks). Spiking experiments verified the accuracy of the method.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan-4, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: T D
authors.initials: TD
authors.lastname: Ninh
authors.forename: Y
authors.initials: Y
authors.lastname: Nagashima
authors.forename: K
authors.initials: K
authors.lastname: Shiomi
mesh_terms: D000818:Animals; D001152:Arsenicals / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D049851:Gastropoda / Q000737:chemistry; D017747:Seafood / Q000032:analysis*; D012758:Shellfish / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D001152:Arsenicals