[Nuclear transfer of goat somatic cells transgenic for human lactoferrin].
Transgenic animal mammary gland bioreactors are being used to produce recombinant proteins with appropriate post-translational modifications, and nuclear transfer of transgenic somatic cells is a more powerful method to produce mammary gland bioreactor. Here we describe efficient gene transfer and nuclear transfer in goat somatic cells. Gene targeting vector pGBC2LF was constructed by cloning human lactoferrin (LF) gene cDNA into exon 2 of the milk goat beta-casein gene, and the endogenous start condon was replaced by that of human LF gene. Goat fetal fibroblasts were transfected with linearized pGBC2LF and 14 cell lines were positive according to PCR and Southern blot. The transgenic cells were used as donor cells of nuclear transfer, and some of reconstructed embryos could develop to blastocyst in vitro.
Li
Lan
L
Institute of Animal Reproduction, Development and Genetic Engineering, Department of Animal Sciences, Laiyang Agriculture College, Qingdao 266109, China.
Shen
Wei
W
Pan
Qing-Yu
QY
Min
Ling-Jiang
LJ
Sun
Yu-Jiang
YJ
Fang
Yong-Wei
YW
Deng
Ji-Xian
JX
Pan
Qing-Jie
QJ
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D006041:Goats / Q000033:anatomy & histology / Q000235:genetics; D006801:Humans; D007781:Lactoferrin / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000378:metabolism; D053652:Nuclear Transfer Techniques; D014162:Transfection
D018076:DNA, Complementary; D007781:Lactoferrin