title: The parthenogenetic activation of canine oocytes with Ca-EDTA by various culture periods and concentrations.
abstract: In the present study, canine oocytes were exposed to various concentrations of and durations of exposure to EDTA saturated with Ca(2+) (Ca-EDTA), a cell membrane-impermeable metal ion chelator, to determine if parthenogenetic activation could be induced. When oocytes were cultured for 48 or 72 h in parthenogenetic activation medium (PAM) without Ca-EDTA (control) or PAM supplemented with 1 or 5mM Ca-EDTA, the highest rate of pronuclear formation (PN) was obtained in oocytes cultured in 1mM Ca-EDTA for 48 h (8.0%; P<0.05). There was no pronuclear formation in the control group (PAM without Ca-EDTA). Oocytes treated with 5mM Ca-EDTA for 48 h or 1mM Ca-EDTA for 72 h formed a parthenogenetic pronucleus (3.1 and 4.5, respectively). However, there was no pronuclear formation in oocytes treated with 5mM Ca-EDTA for 72 h. In summary, exposure to Ca-EDTA can induce pronuclear formation in canine oocytes.
authors.affiliation: School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, Daegu 702-701, Republic of Korea.
authors.forename: Sang Ryeul
authors.initials: SR
authors.lastname: Lee
authors.forename: Jae-Wook
authors.initials: JW
authors.lastname: Kim
authors.forename: Bong Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Myoung Ok
authors.initials: MO
authors.lastname: Kim
authors.forename: Sung Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Dong Hun
authors.initials: DH
authors.lastname: Yoo
authors.forename: Mi-Jung
authors.initials: MJ
authors.lastname: Shin
authors.forename: Sanggyu
authors.initials: S
authors.lastname: Lee
authors.forename: Young Sik
authors.initials: YS
authors.lastname: Park
authors.forename: Yong Bok
authors.initials: YB
authors.lastname: Park
authors.forename: Ji Hong
authors.initials: JH
authors.lastname: Ha
authors.forename: Zae Young
authors.initials: ZY
authors.lastname: Ryoo
mesh_terms: D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology*; D046508:Culture Techniques / Q000662:veterinary*; D004285:Dogs / Q000502:physiology*; D004492:Edetic Acid / Q000494:pharmacology*; D005260:Female; D009865:Oocytes / Q000187:drug effects*; D010312:Parthenogenesis / Q000187:drug effects*; D013997:Time Factors
chemical_list: D002614:Chelating Agents; D004492:Edetic Acid