High expression of survivin and down-regulation of Stat-3 characterize the feto-maternal interface in failing murine pregnancies during the implantation period.
The materno-fetal interface has for long been considered as an immune privileged biological site and thus understanding the mechanisms underlying fetal survival have been the focus of intense research. In adults, survivin and Stat-3 proteins are involved in tolerance as well as the induction of apoptosis. However, the role of these molecules in pregnancy and development has not been addressed. We have evaluated the expression of survivin and Stat-3 in allogeneic mouse models of low abortions (CBA/J x Balb/c), abortion prone (CBA/J x DBA/2J) and stress-triggered abortions from DBA/2J-mated CBA/J mice. We show that survivin is over-expressed in abortion-prone mating on gestation day 7.5. This effect was also found in stress-exposed mice, whereas expression was low in normal pregnancy mice. The phosphorylated Stat-3 (p-Stat-3) was down regulated in high abortion mating compared with low abortion mating, CBA/J x Balb/c. The level of apoptosis was similar in the three groups studied. Our results suggest that high expression of survivin and low expression of p-Stat-3 are involved in pregnancy loss in mice.
Garcia
Mariana G
MG
Charité, Medicine University of Berlin, Biomedical Research Building (BMFZ), Raum 2.0549, Campus Virchow, Augustenburger Platz 1, 13353 Berlin, Germany.
Tirado-Gonzalez
Irene
I
Handjiski
Bori
B
Tometten
Mareike
M
Orsal
Arif S
AS
Hajos
Silvia E
SE
Fernández
Nelson
N
Arck
Petra C
PC
Blois
Sandra M
SM
D000022:Abortion, Spontaneous / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D003656:Decidua / Q000737:chemistry / Q000378:metabolism; D015536:Down-Regulation; D010064:Embryo Implantation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins; D008431:Maternal-Fetal Exchange; D051379:Mice; D008815:Mice, Inbred Strains; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000378:metabolism*; D010920:Placenta / Q000737:chemistry / Q000378:metabolism*; D011247:Pregnancy; D012097:Repressor Proteins; D050796:STAT3 Transcription Factor / Q000032:analysis / Q000378:metabolism*; D000077022:Survivin; D015854:Up-Regulation
C117749:Birc5 protein, mouse; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D012097:Repressor Proteins; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D000077022:Survivin