Solution structure of mouse Cripto CFC domain and its inactive variant Trp107Ala.
We report here for the first time the solution structures at pH 3 and pH 6 of the synthetic CFC domain of mouse Cripto and of the point mutated variant W107A that is unable to bind to the Alk4 Cripto receptor. NMR data confirm that the CFC domain has a C1-C4, C2-C6, C3-C5 disulfide pattern and show that structures are rather flexible and globally extended, with three noncanonical antiparallel strands. His104 and Trp107 side chains protrude from a protein edge and are strongly exposed to solvent, supporting previous evidence of direct involvement in receptor binding. On the opposite molecule side, several nonpolar residues are gathered, forming a large hydrophobic patch that supposedly acts as interface with the cell membrane or the adjacent EGF-like domain. A second hydrophilic patch surrounding His104 and Trp107 is present only in the wild type variant, suggesting a possible involvement in modulating Alk4 recognition.
Calvanese
Luisa
L
Dipartimento di Chimica, Università Federico II, Complesso Universitario MSA, via Cintia 45, 80126 Napoli, Italy.
Saporito
Angela
A
Marasco
Daniela
D
D'Auria
Gabriella
G
Minchiotti
Gabriella
G
Pedone
Carlo
C
Paolillo
Livio
L
Falcigno
Lucia
L
Ruvo
Menotti
M
D000595:Amino Acid Sequence; D000818:Animals; D004815:Epidermal Growth Factor / Q000737:chemistry* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000235:genetics; D017354:Point Mutation; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012996:Solutions
D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D012996:Solutions; C489450:Tdgf1 protein, mouse; D004815:Epidermal Growth Factor