Hexadecanedionic acid-sepharose 4B: A new tool for preparation of albumin-depleted plasma.
Serum and plasma are the major sources of human material for clinical molecular diagnostics and drug discovery. However, due to the high abundance of some proteins, of which serum albumin (SA) is most prominent, lower-abundance proteins often remain undetectable in proteomic analysis of these body fluids. We have used hexadecanedionic acid (HDA) immobilized to Sepharose 4B to develop an affinity resin that is effective in the removal of SA from plasma. Two-dimensional gel analysis of the SA-depleted samples shows a significant enhancement of the low-abundance proteins and highly specific capture of serum albumin. The HDA resin shows better performance in terms of specificity than dye-based resins.
Soskic
Vukic
V
ProteoSys AG, Carl-Zeiss-Strasse 51, 55129 Mainz, Germany. vukic.soskic@proteosys.com
Schwall
Gerhard
G
Nyakatura
Elke
E
Poznanovic
Slobodan
S
Stegmann
Werner
W
Schrattenholz
Andre
A
D000345:Affinity Labels / Q000737:chemistry*; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D010169:Palmitic Acids; D012685:Sepharose; D044967:Serum / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000345:Affinity Labels; D010169:Palmitic Acids; D012709:Serum Albumin; C012346:hexadecanedioic acid; D012685:Sepharose