title: Involvement of the Vibrio parahaemolyticus pvsC gene in export of the siderophore vibrioferrin.
abstract: The pvsC gene of unknown function has been found in the iron-regulated vibrioferrin biosynthesis operon of Vibrio parahaemolyticus (Tanabe, T. et al., J. Bacteriol. 185: 6938-6949, 2003). The amino acid sequence deduced from the gene showed significant similarity to 12-transmembrane segment efflux pumps belonging to the major facilitator superfamily. A nonpolar deletion of pvsC in V. parahaemolyticus resulted in a reduced release of vibrioferrin into the medium. Vibrioferrin release could be regained by introducing the intact pvsC gene on a complementing plasmid. These results indicate that the pvsC gene product functions as an inner membrane exporter of vibrioferrin.
authors.affiliation: Department of Molecular Biopharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: Tomotaka
authors.initials: T
authors.lastname: Tanabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakao
authors.forename: Teruo
authors.initials: T
authors.lastname: Kuroda
authors.forename: Tomofusa
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D002951:Citrates / Q000378:metabolism*; D017077:Culture Media, Conditioned; D008969:Molecular Sequence Data; D011760:Pyrrolidinones / Q000378:metabolism*; D016415:Sequence Alignment; D017262:Siderophores / Q000378:metabolism*; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002951:Citrates; D017077:Culture Media, Conditioned; D011760:Pyrrolidinones; D017262:Siderophores; C081902:pmrA protein, Bacteria; C090126:vibrioferrin