title: Analgesic activity of affinin, an alkamide from Heliopsis longipes (Compositae).
abstract: Heliopsis longipes (Compositae) is a Mexican plant used as analgesic in pain toothache. A solution of 10mug/ml of dichloromethane extract from this plant showed analgesic activity determined by means of GABA release in mice brain slices. Through a bioassay-directed separation, fractions G-1, G-2, G-4 and G-6 at the same concentration were active. Affinin was the unique and common active compound, and evoke the GABA release 0.5min after administration at 1x10(-4)M concentration. Inactive compound were undeca-2E-en-8,10-dyinoic acid isobutylamide, hinokinin, 2'-hydroxyhinokinin, 3beta-sn-glyceroyl-(1''-palmitoxy)urs-12-ene, 13(18)-ursen-3beta-ol, 13(18)-ursen-3beta-acetate, beta-sitosterol and stigmasterol. The analgesic activity of Heliopsis longipes could be associated to affinin.
authors.affiliation: Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, Mexico. myolanda@uaem.mx
authors.forename: María Yolanda
authors.initials: MY
authors.lastname: Rios
authors.forename: A Berenice
authors.initials: AB
authors.lastname: Aguilar-Guadarrama
authors.forename: María Del Carmen
authors.initials: Mdel C
authors.lastname: Gutiérrez
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000302:isolation & purification; D000475:Alkenes / Q000302:isolation & purification / Q000494:pharmacology*; D000577:Amides / Q000302:isolation & purification / Q000494:pharmacology*; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D052117:Benzodioxoles; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004149:Dioxoles / Q000302:isolation & purification; D005260:Female; D066298:In Vitro Techniques; D017705:Lignans / Q000302:isolation & purification; D008519:Medicine, Traditional; D008752:Methylene Chloride; D008800:Mexico; D051379:Mice; D010146:Pain / Q000188:drug therapy*; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D053284:Polyunsaturated Alkamides / Q000302:isolation & purification / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000187:drug effects / Q000378:metabolism
chemical_list: D000475:Alkenes; D000577:Amides; D000700:Analgesics; D052117:Benzodioxoles; D004149:Dioxoles; D017705:Lignans; C489347:N-isobutyl-2E-decenamide; D010936:Plant Extracts; D053284:Polyunsaturated Alkamides; C475934:hinokinin; D005680:gamma-Aminobutyric Acid; D008752:Methylene Chloride; D015107:4-Butyrolactone