DNA-binding and cleavage studies of novel binuclear copper(II) complex with 1,1'-dimethyl-2,2'-biimidazole ligand.
DNA-binding properties of novel binulear copper(II) complex [Cu(2)(Dmbiim)(4)(H(2)O)(2)](ClO(4))(4).6H(2)O, where Dmbiim = 1,1'-Dimethyl-2,2'-biimidazole are investigated using electronic absorption spectroscopy, fluorescence spectroscopy, viscosity measurement and voltammetry. The results show that the copper(II) complex interacts with DNA through minor groove binding. The interaction between the complex and DNA has also been investigated by gel electrophoresis, interestingly, we found that the copper(II) complex can cleave circular plasmid pBR322 DNA efficiently in the presence of AH(2) (ascorbic acid) at pH 8.0 and 37 degrees C.
Li
Yanping
Y
Institute of Molecular Science, Chemical Biology and Molecular Engineering, Laboratory of Education Ministry, Shanxi University, Taiyuan 030006, PR China.
Wu
Yanbo
Y
Zhao
Jing
J
Yang
Pin
P
D001483:Base Sequence; D001665:Binding Sites; D003300:Copper / Q000378:metabolism / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D004563:Electrochemistry; D007093:Imidazoles / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D008958:Models, Molecular; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010957:Plasmids / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D014783:Viscosity
D007093:Imidazoles; D009942:Organometallic Compounds; D003300:Copper; D004247:DNA