Time-resolved fluorescence imaging of islet cell autoantibodies.
Time-resolved fluorescence of lanthanide chelates has been widely used in bioanalytical assays. Long fluorescence time, large Stokes shift, and minute fading out of the fluorescence over years are major advantages of the lanthanides over the conventional fluorescent dyes. We have now applied time-resolved fluorescence imaging (TRFI) also for measurement of type 1 diabetes mellitus (T1DM)-related islet cell autoantibodies (ICA). Retaining the accuracy of conventional ICA, TRFI has over 10 times better signal-to-noise ratio than the conventional fluorochromes. The technology allows objective determination of fluorescence intensity with the camera and computer software, and serial dilutions for obtaining the antibody titer in autoantibody-positive samples are unnecessary. We now describe the TRFI as a method and its application for measurement of ICA.
Vuorinen
Pauli
P
The JDRF Center for the Prevention of Type I Diabetes in Finland, and University of Turku, Department of Pediatrics, Lemminkaisenkatu 14-18 A 5.krs, 20520 Turku, Finland. pauli.vuorinen@utu.fi
Rulli
Maris
M
Kuusisto
Ari
A
Simell
Satu
S
Simell
Tuula
T
Vahlberg
Tero
T
Ilonen
Jorma
J
Hyöty
Heikki
H
Knip
Mikael
M
Simell
Olli
O
D001323:Autoantibodies / Q000032:analysis* / Q000097:blood; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007515:Islets of Langerhans / Q000276:immunology*; D028581:Lanthanoid Series Elements / Q000032:analysis; D013997:Time Factors
D001323:Autoantibodies; D028581:Lanthanoid Series Elements