title: Monoclonal C5-1 antibody produced in transgenic alfalfa plants exhibits a N-glycosylation that is homogenous and suitable for glyco-engineering into human-compatible structures.
abstract: Structural analysis of the N-glycosylation of alfalfa proteins was investigated in order to evaluate the capacity of this plant to perform this biologically important post-translational modification. We show that, in alfalfa, N-linked glycans are processed into a large variety of mature oligosaccharides having core-xylose and core alpha(1,3)-fucose, as well as terminal Lewis(a) epitopes. In contrast, expression of the C5-1 monoclonal antibody in alfalfa plants results in the production of plant-derived IgG1 which is N-glycosylated by a predominant glycan having a alpha(1,3)-fucose and a beta(1,2)-xylose attached to a GlcNAc2Man3GlcNAc2 core. Since this core is common to plant and mammal N-linked glycans, it therefore appears that alfalfa plants have the ability to produce recombinant IgG1 having a N-glycosylation that is suitable for in vitro or in vivo glycan remodelling into a human-compatible plantibody. For instance, as proof of concept, in vitro galactosylation of the alfalfa-derived C5-1 mAb resulted in a homogenous plantibody harbouring terminal beta(1,4)-galactose residues as observed in the mammalian IgG.
authors.affiliation: CNRS-UMR 6037, IFRMP 23, Université de Rouen, 76821 Mont Saint Aignan, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Bardor
authors.forename: Corinne
authors.initials: C
authors.lastname: Loutelier-Bourhis
authors.forename: Thomas
authors.initials: T
authors.lastname: Paccalet
authors.forename: Pascal
authors.initials: P
authors.lastname: Cosette
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Fitchette
authors.forename: Louis-P
authors.initials: LP
authors.lastname: Vézina
authors.forename: Sonia
authors.initials: S
authors.lastname: Trépanier
authors.forename: Michèle
authors.initials: M
authors.lastname: Dargis
authors.forename: Réal
authors.initials: R
authors.lastname: Lemieux
authors.forename: Catherine
authors.initials: C
authors.lastname: Lange
authors.forename: Loïc
authors.initials: L
authors.lastname: Faye
authors.forename: Patrice
authors.initials: P
authors.lastname: Lerouge