title: Expression of CD27 on peripheral CD4+ T-lymphocytes correlates with the development of severe acute graft-versus-host disease after allogeneic bone marrow transplantation.
abstract: Allogeneic immune responses during hematopoietic reconstitution play central roles in beneficial and adverse effects after allogeneic bone marrow transplantation (allo-BMT). Appropriate regulation of the immune responses might improve the outcome of allo-BMT. However, a useful marker for monitoring allogeneic immune responses remains to be established. We enrolled 22 consecutive patients who underwent myeloablative allo-BMT between March 2002 and March 2006 and examined the relationship between CD27 expression on peripheral blood T-lymphocytes, a possible marker for naive/effector phenotypes, and clinical events, especially acute graft-versus-host disease (aGVHD). In 8 patients with aGVHD of grades II to IV, the CD27+/CD27- ratios of CD4+ (but not CD8+) T-lymphocytes were significantly higher after allo-BMT, even at day 21, than the ratios in patients with aGVHD of grade 0 or I and remained high after day 21. In contrast, the ratios were low after day 21 following allo-BMT in 14 patients with aGVHD of grade 0 or I. Moreover, the clinical analysis suggested a relationship between the ratio and aGVHD grade. Thus, we showed that the CD27+/CD27- ratio in CD4+ T-lymphocytes may have value in predicting the development of severe aGVHD and may correlate with clinical symptoms of aGVHD.
authors.affiliation: Department of Hematology and Oncology, Graduate School of Medicine, Osaka University, Osaka, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Maeda
authors.forename: Jun
authors.initials: J
authors.lastname: Ishikawa
authors.forename: Shinya
authors.initials: S
authors.lastname: Inoue
authors.forename: Hitomi
authors.initials: H
authors.lastname: Matsunaga
authors.forename: Satoru
authors.initials: S
authors.lastname: Kosugi
authors.forename: Masamichi
authors.initials: M
authors.lastname: Shiraga
authors.forename: Kenji
authors.initials: K
authors.lastname: Oritani
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Kanakura
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tomiyama
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry*; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis*; D006801:Humans; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012720:Severity of Illness Index; D013997:Time Factors; D014181:Transplantation Immunology; D014184:Transplantation, Homologous; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000032:analysis*
chemical_list: D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7