DNA damage in mouse lymphocytes exposed to curcumin and copper.
Dietary polyphenolics, such as curcumin, have shown antioxidant and anti-inflammatory effects. Some antioxidants cause DNA strand breaks in excess of transition metal ions, such as copper. The aim of this study was to evaluate the in vitro effect of curcumin in the presence of increasing concentrations of copper to induce DNA damage in murine leukocytes by the comet assay. Balb-C mouse lymphocytes were exposed to 50 microM curcumin and various concentrations of copper (10 microM, 100 microM and 200 microM). Cellular DNA damage was detected by means of the alkaline comet assay. Our results show that 50 microM curcumin in the presence of 100-200 microM copper induced DNA damage in murine lymphocytes. Curcumin did not inhibit the oxidative DNA damage caused by 50 microM H2O2 in mouse lymphocytes. Moreover, 50 microM curcumin alone was capable of inducing DNA strand breaks under the tested conditions. The increased DNA damage by 50 mM curcumin was observed in the presence of various concentrations of copper, as detected by the alkaline comet assay.
Urbina-Cano
Patricia
P
Laboratorio de Citogenética Genotoxicidad y Biomonitoreo, Instituto de Genética Humana Dr. Enrique Corona Rivera, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Jalisco, Mexico.
Bobadilla-Morales
Lucina
L
Ramírez-Herrera
Mario A
MA
Corona-Rivera
Jorge R
JR
Mendoza-Magaña
Maria L
ML
Troyo-Sanromán
Rogelio
R
Corona-Rivera
Alfredo
A
D000818:Animals; D020552:Comet Assay; D003300:Copper / Q000633:toxicity*; D003474:Curcumin / Q000633:toxicity*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005260:Female; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C
D003300:Copper; D003474:Curcumin; C029892:cupric chloride