title: Proteinase inhibitors from Streptomyces with antiviral activity.
abstract: An extensive screening study for the production of proteolytic inhibitors has been carried out on 75 Streptomyces strains. It was found that 18 of the strains and/or their variants (24%) produced proteinaceous substances, which belonged to the group of typical serine protease inhibitors. 23 samples were tested for inhibitory activity on the replication of influenza virus A/Germany/34, strain Rostock (H7N1) (A/Rostock) in chicken embryonic fibroblast (CEF) cells. Eleven of the tested samples (52.2%) significantly inhibited viral growth. Further the specific inhibitory effect on the replication of influenza virus A/Aichi/2/68 (H3N2) (A/Aichi) in Madin-Darby canine kidney (MDCK) cells and on the growth of herpes simplex virus type 1, strain DA (HSV-1) in Madin-Darby bovine kidney (MDBK) cells was tested. Nine samples significantly inhibited A/Aichi and four - HSV-1. The most effective inhibitors, produced by Streptomyces sp. 225b (SS 225b) and Streptomyces chromofuscus 34-1 (SS 34-1) protected mice from mortality in the experimental influenza A/Aichi virus infection.
authors.affiliation: Institute of Microbiology, Bulgarian Academy of Sciences, Bulgaria. jserkedjieva@microbio.bas.bg
authors.forename: Julia
authors.initials: J
authors.lastname: Serkedjieva
authors.forename: Lidiya
authors.initials: L
authors.lastname: Angelova
authors.forename: Mimi
authors.initials: M
authors.lastname: Remichkova
authors.forename: Iskra
authors.initials: I
authors.lastname: Ivanova
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D002642:Chick Embryo; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D005285:Fermentation; D005347:Fibroblasts; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000254:growth & development; D053122:Influenza A Virus, H3N2 Subtype / Q000187:drug effects / Q000254:growth & development; D051379:Mice; D008813:Mice, Inbred ICR; D009976:Orthomyxoviridae Infections / Q000821:virology*; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D011480:Protease Inhibitors