title: Not all cytokine-producing CD8+ T cells suppress simian immunodeficiency virus replication.
abstract: Current assays of CD8+ T-lymphocyte function measure cytokine production rather than the ability of these lymphocytes to suppress viral replication. Here we show that CD8+ T-cell clones recognizing the same epitope vary enormously in the ability to suppress simian immunodeficiency virus SIVmac239 replication in an in vitro suppression assay. However, all Nef(165-173)IW9- and Vif(66-73)HW8-specific clones from elite controllers effectively suppressed SIV replication. Interestingly, in vitro suppression efficacy was not always associated with the ability to produce gamma interferon, tumor necrosis factor alpha, or interleukin-2.
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715-1299, USA.
authors.forename: Chungwon
authors.initials: C
authors.lastname: Chung
authors.forename: Wonhee
authors.initials: W
authors.lastname: Lee
authors.forename: John T
authors.initials: JT
authors.lastname: Loffredo
authors.forename: Benjamin
authors.initials: B
authors.lastname: Burwitz
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Friedrich
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Giraldo Vela
authors.forename: Gnankang
authors.initials: G
authors.lastname: Napoe
authors.forename: Eva G
authors.initials: EG
authors.lastname: Rakasz
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Wilson
authors.forename: David B
authors.initials: DB
authors.lastname: Allison
authors.forename: David I
authors.initials: DI
authors.lastname: Watkins
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis*; D008253:Macaca mulatta; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000502:physiology; D019562:Viral Load; D014779:Virus Replication / Q000276:immunology* / Q000502:physiology
chemical_list: D016207:Cytokines