title: Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens.
abstract: Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitivities and specificities.
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Kwannan
authors.initials: K
authors.lastname: Nantavisai
authors.forename: Mathirut
authors.initials: M
authors.lastname: Mungthin
authors.forename: Peerapan
authors.initials: P
authors.lastname: Tan-ariya
authors.forename: Ram
authors.initials: R
authors.lastname: Rangsin
authors.forename: Tawee
authors.initials: T
authors.lastname: Naaglor
authors.forename: Saovanee
authors.initials: S
authors.lastname: Leelayoova
mesh_terms: D000818:Animals; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005374:Filtration / Q000379:methods; D005872:Giardia / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D005873:Giardiasis / Q000469:parasitology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012335:RNA, Ribosomal / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan; D012335:RNA, Ribosomal