Effects of ischemia-reperfusion injury on apolipoprotein M expression in the liver.
The present study investigated the expression pattern of apolipoprotein M (apoM) mRNA in a rat model of hepatic ischemia-reperfusion injury (IRI). Animals were ischemic for 1 hour followed by various reperfusion times. As expected, serum alanine aminotransferase levels were significantly increased under IRI, which indicated the severity of liver injury. Hepatic mRNA levels of HSP70, which is the most common characterized protein within the family of heat-shock proteins (HSP), were significantly increased after 0.5 to 3 hours of IRI. Plasma C-reactive protein, high-density lipoprotein-cholesterol, and lipoprotein (a) levels were significantly increased after 1-hour ischemia followed by 0.5 to 3 hours of reperfusion. Interestingly, similar to HSP70, apoM mRNA levels in the liver were gradually increased after 0.5 to 3 hours of IRI, whereas it returned to a lower level after 6 or 24 hours of IRI, which indicated that hepatic apoM expression was significantly influenced by the acute phase of IRI. However, plasma apoM levels were not increased in parallel, even slightly decreasing after 0.5 or 1 hour of IRI. We concluded that apoM mRNA expression pattern, like HSP70, in the liver showed rapid, significant changes during hepatic local IRI.
Xu
X
X
Research Institute of Organ Transplantation, The Affiliated TongJi Hospital of Huazhong University of Science and Technology, Wuhan, China.
Ye
Q
Q
Xu
N
N
He
X
X
Luo
G
G
Zhang
X
X
Zhu
J
J
Zhang
Y
Y
Nilsson-Ehle
P
P
D000818:Animals; D001053:Apolipoproteins / Q000235:genetics* / Q000378:metabolism; D000075243:Apolipoproteins M; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D054834:Lipocalins; D008099:Liver / Q000378:metabolism*; D008102:Liver Circulation; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion / Q000379:methods; D015427:Reperfusion Injury / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C400523:APOM protein, human; D001053:Apolipoproteins; D000075243:Apolipoproteins M; D018840:HSP70 Heat-Shock Proteins; D054834:Lipocalins; D012333:RNA, Messenger