Identification of differentially expressed genes in omental adipose tissues of obese patients by suppression subtractive hybridization.
To identify differentially expressed genes between obese individuals and normal control, we have undertaken suppression subtractive hybridization (SSH). Omental adipose tissues were obtained via abdominal surgery for appendicitis in both 13 obese subjects [BMI (body mass index) >30 kg/m2] and 13 normal subjects (BMI >18 and <25 kg/m2). Following SSH, about one thousand clones were sequenced and found to derive from 426 different genes. These predominately expressed genes included genes involved in lipid metabolism, cytokines, signal transduction, GLUT4 translocation, cell cycle and growth, cytoskeleton, and others. Although more detailed analyses are necessary, it is anticipated that further study of genes identified will provide insights into their specific roles in the etiology of obesity.
Qiu
Jie
J
Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210029, China.
Ni
Yu-hui
YH
Gong
Hai-xia
HX
Fei
Li
L
Pan
Xiao-qin
XQ
Guo
Mei
M
Chen
Rong-hua
RH
Guo
Xi-rong
XR
D000273:Adipose Tissue / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D009852:Omentum / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*
D020543:Proteome