title: Content of glycogen and trehalose and activity of alpha-amylase and trehalase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae.
abstract: BACKGROUND
  The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on metabolism of glycogen and trehalose of the host was studied.
  
  
  METHODS
  Last instar larvae (L7) of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae. At 6, 12, 18 and 24 h after infection concentrations of trehalose and glycogen as well as activity of trehalase and alpha-amylase were determined.
  
  
  RESULTS
  The content of glycogen was lower in insects infected with S. feltiae than in the controls and animals infected with S. affinis. The content of trehalose was higher in insects from both infected groups than in the controls. Its concentration was slightly higher in larvae infected with S. affinis than in those infected with S. feltiae. The activity of alpha-amylase after infection with S. affinis was low. It was significantly higher in insects infected with S. feltiae. In animals of both infected groups, following a significant reduction at 6 h, the activity of trehalase remained at a similar level, higher than in the controls. In the paper the effects of infection with (i) different species of entomopathogenic nematodes and (ii) the importance of the developmental stage of the insect-host for changes in its metabolism of glycogen and trehalose were discussed.
authors.affiliation: Department of Biochemistry, Faculty of Biology, University of Warmia and Mazury, Oczapowskiego lA St., 10-719 Olsztyn, Poland. k.zoltowska@uwm.edu.pl
authors.forename: Krystyna
authors.initials: K
authors.lastname: Zółtowska
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Lopieńiska-Biernat
mesh_terms: D000818:Animals; D006003:Glycogen / Q000032:analysis / Q000378:metabolism*; D006790:Host-Parasite Interactions; D007814:Larva / Q000737:chemistry / Q000378:metabolism / Q000469:parasitology; D009036:Moths / Q000737:chemistry / Q000378:metabolism* / Q000469:parasitology*; D010572:Pest Control, Biological / Q000379:methods; D017168:Rhabditida / Q000502:physiology*; D014198:Trehalase / Q000032:analysis / Q000378:metabolism*; D014199:Trehalose / Q000032:analysis / Q000378:metabolism*; D000516:alpha-Amylases / Q000032:analysis / Q000378:metabolism*
chemical_list: D006003:Glycogen; D014199:Trehalose; D000516:alpha-Amylases; D014198:Trehalase