Enhancement of growth and polysaccharide production in suspension cultures of protocorm-like bodies from Dendrobium huoshanense by the addition of putrescine.
Putrescine at 0.6 mM stimulated protocorm-like body growth and polysaccharide synthesis in suspension cultures of Dendrobium huoshanense. The specific growth rate of protocorm-like body increased from 0.047 to 0.056 day(-1), and the maximum dry weight and polysaccharide production reached 33.2 and 2.94 g l(-1), respectively, while they were 24.6 and 2.12 g l(-1), respectively, in the control. The administration of polyamine inhibitor, alpha-DL-difluoromethylarginine, at 1 mM, decreased protocorm-like body growth and polysaccharide production to 21.4 and 1.76 g l(-1), respectively.
Wei
Ming
M
Department of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, PR China.
Jiang
Shao-Tong
ST
Luo
Jian-Ping
JP
D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D031667:Dendrobium / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D018525:Germination / Q000187:drug effects / Q000502:physiology; D011134:Polysaccharides / Q000096:biosynthesis*; D011700:Putrescine / Q000008:administration & dosage*; D012639:Seeds / Q000187:drug effects / Q000502:physiology*
D011134:Polysaccharides; D011700:Putrescine