title: Protective immunity in grouper (Epinephelus coioides) following exposure to or injection with Cryptocaryon irritans.
abstract: The protective immunity of grouper (Epinephelus coioides) against Cryptocaryon irritans was determined after immunisation by surface exposure or intraperitoneal injection. Specific antibody titres of immunised fish serum and skin culture supernatant were determined by enzyme-linked immunosorbent assay and immobilisation assays. Specific antibody can be detected in some immunised fish at Week 1 and in all immunised fish at Week 2, and the peaks were between Weeks 4-6. Specific antibody was still evident in the serum and skin of immunised fish at Week 8, and provided good protection against challenge with C. irritans. These findings indicated that humoral and skin mucosal immunity play important roles in fish against C. irritans infection.
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Sciences, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, PR China.
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Luo
authors.forename: Ming-Quan
authors.initials: MQ
authors.lastname: Xie
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
authors.forename: An-Xing
authors.initials: AX
authors.lastname: Li
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D016798:Ciliophora / Q000276:immunology*; D016770:Ciliophora Infections / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology* / Q000469:parasitology; D018928:Immunity, Mucosal / Q000276:immunology; D007114:Immunization / Q000662:veterinary; D007274:Injections, Intraperitoneal; D010473:Perciformes / Q000276:immunology*; D012867:Skin / Q000276:immunology; D013997:Time Factors
chemical_list: D000913:Antibodies, Protozoan