title: Steroid-1-dehydrogenase of Mycobacterium sp. VKM Ac-1817D strain producing 9alpha-hydroxy-androst-4-ene-3,17-dione from sitosterol.
abstract: The strain of Mycobacterium sp. VKM Ac-1817D forms 9alpha-hydroxy-androst-4-ene-3,17-dione (9-OH-AD) as a major product from sitosterol. The formation of 9-OH-AD was accompanied with its partial destruction due to residual steroid-1-dehydrogenase (St1DH) activity. The activity was found to be induced by androst-4-ene-3,17-dione (AD), while other intermediates of sitosterol oxidation did not influence 1(2)-dehydrogenation. The enzyme is located mainly in the cytosolic fraction. The cytosolic St1DH (dimer, M (r) approximately 58 kDa) was partially purified by ammonium sulfate fractionation, ion-exchange chromatography on DEAE-Sepharose and Phenyl-Sepharose, and gel filtration on Bio-Gel A-0.5M. It expressed the St1DH activity toward both AD and 9-OH-AD.
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia.
authors.forename: G V
authors.initials: GV
authors.lastname: Sukhodolskaya
authors.forename: V M
authors.initials: VM
authors.lastname: Nikolayeva
authors.forename: S M
authors.initials: SM
authors.lastname: Khomutov
authors.forename: M V
authors.initials: MV
authors.lastname: Donova
mesh_terms: D000645:Ammonium Sulfate; D000735:Androstenedione / Q000031:analogs & derivatives* / Q000096:biosynthesis; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000737:chemistry; D004790:Enzyme Induction; D008954:Models, Biological; D015394:Molecular Structure; D008970:Molecular Weight; D009161:Mycobacterium / Q000201:enzymology* / Q000235:genetics; D010088:Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism*; D012855:Sitosterols / Q000378:metabolism*
chemical_list: D012855:Sitosterols; D000735:Androstenedione; C076717:9-hydroxy-4-androstene-3,17-dione; C025473:gamma-sitosterol; D010088:Oxidoreductases; C022656:3-oxosteroid delta(1) dehydrogenase; D000645:Ammonium Sulfate