title: Dialysis membrane-induced oxidative stress: role of heme oxygenase-1.
abstract: Dialysis membranes have been reported to induce monocyte apoptosis. We studied the role of oxidative stress in the induction of dialysis membrane-induced monocyte apoptosis. Superoxide dismutase, a superoxide scavenger, prevented dialysis membrane-induced monocyte apoptosis. Similarly, other antioxidants also inhibited dialysis membrane- induced apoptosis. In addition, the interaction of dialysis membranes with monocytes was associated with the generation of molecules leading to oxidative stress such as superoxide and TBARS. Interestingly, pre-induction of heme oxygenase (HO)-1 by hemin prevented dialysis membrane-induced monocyte apoptosis, whereas inhibition of HO-1 activity (treatment with tin protoporphyrin, SN-P) enhanced dialysis membrane-induced monocyte apoptosis. We suggest that oxidative injury associated with dialysis membrane and monocyte interaction plays a role in monocyte injury. Pre-induction of HO-1 may attenuate dialysis membrane-induced monocyte apoptosis.
authors.affiliation: Division of Kidney Diseases and Hypertension, North Shore University Hospital, Manhasset and Long Island Jewish Medical Center, New Hyde Park, NY, USA.
authors.forename: Madhu
authors.initials: M
authors.lastname: Bhaskaran
authors.forename: Neetu
authors.initials: N
authors.lastname: Radhakrishnan
authors.forename: Hitesh
authors.initials: H
authors.lastname: Patni
authors.forename: Priyanka
authors.initials: P
authors.lastname: Singh
authors.forename: Ahmad N
authors.initials: AN
authors.lastname: Chaudhary
authors.forename: Pravin C
authors.initials: PC
authors.lastname: Singhal
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis; D002482:Cellulose / Q000031:analogs & derivatives / Q000494:pharmacology; D053938:DNA Fragmentation; D051547:Heme Oxygenase-1 / Q000502:physiology*; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D015227:Lipid Peroxidation; D008567:Membranes, Artificial; D009000:Monocytes / Q000378:metabolism*; D018384:Oxidative Stress; D006435:Renal Dialysis / Q000009:adverse effects*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008567:Membranes, Artificial; D013481:Superoxides; C005062:acetylcellulose; D006427:Hemin; D002482:Cellulose; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase