Novel fluorescent proteins for high-content screening.
The development of fast microscopic imaging devices has enabled the application of automated fluorescence microscopy to pharmaceutical high-throughput drug-discovery assays, referred to as high-content screening (HCS). Initially, green fluorescent protein and its derivatives from Aequorea Victoria, and later anthozoan fluorescent proteins (FPs) have become potent tools as live-cell markers in HCS assays. We illustrate the broad applicability of classic and novel FPs to drug-discovery assays, giving example applications of the use of FPs in multiplexed imaging as fluorescent timers, photosensitizers and pulse-chase labels, and for robotically integrated compound testing.
Wolff
Michael
M
Department of Lead Discovery, Boehringer Ingelheim Pharma GmbH and Co. KG, Birkendorfer Str.65, D-88397 Biberach, Germany.
Wiedenmann
Joerg
J
Nienhaus
G Ulrich
GU
Valler
Martin
M
Heilker
Ralf
R
D000818:Animals; D015195:Drug Design; D004353:Drug Evaluation, Preclinical; D008163:Luminescent Measurements / Q000379:methods; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism*; D013678:Technology, Pharmaceutical / Q000379:methods; D000097573:Red Fluorescent Protein
D008164:Luminescent Proteins; D004364:Pharmaceutical Preparations; D000097573:Red Fluorescent Protein