High-throughput analysis of mumps virus and the virus-specific monoclonal antibody on the arrays of a cationic polyelectrolyte with a spectral SPR biosensor.
We investigated the potential use of a spectral surface plasmon resonance (SPR) biosensor in a high-throughput analysis of mumps virus and a mumps virus-specific mAb on the arrays of a cationic polyelectrolyte, poly(diallyldimethylammonium chloride) (PDDA). The PDDA surface was constructed by electrostatic adsorption of the polyelectrolyte onto a monolayer of 11-mercaptoundecanoic acid (MUA). Poly-L-lysine was also adsorbed onto the MUA monolayer and compared with the PDDA surface in the capacity of mumps virus immobilization. The PDDA surface showed a higher adsorption of mumps virus than the poly-L-lysine surface. The SPR signal caused by the virus binding onto the PDDA surface was proportional to the concentration of mumps virus from 0.5 x 10(5) to 14 x 10(5) pfu/mL. The surface structure of the virus arrays was visualized by atomic force microscopy. Then, a dose-dependent increase in the SPR signal was observed when various concentrations of the antimumps virus antibody in buffer or human serum were applied to the virus arrays, and their interaction was specific. Thus, it is likely that the spectral SPR biosensor based on the cationic polyelectrolyte surface may provide an efficient system for a high-throughput analysis of intact virus and serodiagnosis of infectious diseases.
Kim
Hyun-Soo
HS
Department of Molecular and Cellular Biochemistry, Kangwon National University College of Medicine, Chuncheon, Kangwon-do, Korea.
Jung
Se-Hui
SH
Kim
Sang-Hyun
SH
Suh
In-Bum
IB
Kim
Woo Jin
WJ
Jung
Jae-Wan
JW
Yuk
Jong Seol
JS
Kim
Young-Myeong
YM
Ha
Kwon-Soo
KS
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood; D006801:Humans; D018625:Microscopy, Atomic Force; D009107:Mumps / Q000175:diagnosis / Q000276:immunology; D009109:Mumps virus / Q000276:immunology* / Q000302:isolation & purification*; D011073:Polyamines; D000071228:Polyelectrolytes; D011095:Polyethylenes; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods; D000644:Quaternary Ammonium Compounds; D012698:Serologic Tests; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D011073:Polyamines; D000071228:Polyelectrolytes; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride