title: Ageing in yeast does not enhance prion generation.
abstract: The yeast prions [URE3] and [PSI(+)] are self-propagating amyloids of Ure2p and Sup35p, respectively. The analogous transmissible spongiform encephalopathies of mammals and other amyloidoses are largely diseases of later life. From normal strains lacking the prions, we isolated old cells and measured the frequency of de novo [URE3] and [PSI(+)] prion generation. We find no evidence that ageing of yeast increases the frequency of prion occurrence.
authors.affiliation: Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0830, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: Shewmaker
authors.forename: Reed B
authors.initials: RB
authors.lastname: Wickner
mesh_terms: D000682:Amyloid / Q000378:metabolism; D005979:Glutathione Peroxidase; D010454:Peptide Termination Factors; D011328:Prions / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000682:Amyloid; D010454:Peptide Termination Factors; D011328:Prions; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D005979:Glutathione Peroxidase; C067020:URE2 protein, S cerevisiae