Optimization of CZE for analysis of phytochemical bioactive compounds.
Advantages of CZE such as high efficiency, low cost, short analysis time, and easy implementation result in its wide applications for analysis of phytochemical bioactive compounds (e.g. flavonoids, alkaloids, terpenoids, phenolic acid, saponins, anthraquinones and coumarins). However, several aspects, including sample preparation, separation, and detection have significant effects on CZE analysis. Therefore, optimization of these procedures is necessary for development of the method. In this review, sample preparation such as extraction method and preconcentration, separation factors including buffer type, concentration and pH, additives, voltage and temperature, as well as detection, e.g. direct and indirect UV detection, LIF and MS were discussed for optimization of CZE analysis on phytochemical bioactive compounds. The optimized strategies were also reviewed.
Li
Peng
P
Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau, China.
Li
Shao Ping
SP
Wang
Yi Tao
YT
D001688:Biological Products / Q000032:analysis / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards*; D028321:Plant Preparations / Q000737:chemistry*; D013057:Spectrum Analysis
D001688:Biological Products; D028321:Plant Preparations