title: Gene expression profiling of early involuting mammary gland reveals novel genes potentially relevant to human breast cancer.
abstract: Mammary gland involution represents one of the most dramatic examples of programmed cell death/apoptosis and tissue regression during development, yet large gaps still exist in the understanding of the mechanisms involved, and the key factors that trigger involution, are not yet identified. With the focus on identifying "novel" genes associated with mammary gland regression, we used microarray analysis to examine differentially expressed genes during early mammary gland involution in the mouse. We then examined the relevance of candidate genes to human tumorigenesis and identified a number of genes not previously implicated or not well characterized in human breast cancer. The expression levels of these genes in human breast cancer were confirmed in breast cancer cell lines and breast tumor tissues. This pilot study demonstrates proof of principle that through the analysis of gene expression during mammary gland involution, it may be possible to identify "novel" genes relevant human breast cancer.
authors.affiliation: University of Manitoba, Department of Pathology, Winnipeg, Manitoba, Canada.
authors.forename: Anne
authors.initials: A
authors.lastname: Blanchard
authors.forename: Robert
authors.initials: R
authors.lastname: Shiu
authors.forename: Stephanie
authors.initials: S
authors.lastname: Booth
authors.forename: Garrett
authors.initials: G
authors.lastname: Sorensen
authors.forename: Nicole
authors.initials: N
authors.lastname: DeCorby
authors.forename: Andreea
authors.initials: A
authors.lastname: Nistor
authors.forename: Paul
authors.initials: P
authors.lastname: Wong
authors.forename: Etienne
authors.initials: E
authors.lastname: Leygue
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Myal
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D062445:Claudin-1; D005260:Female; D020869:Gene Expression Profiling; D052138:Genes, Neoplasm; D006801:Humans; D008321:Mammary Glands, Animal / Q000254:growth & development / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction
chemical_list: C568108:CLDN1 protein, human; D062445:Claudin-1; C568113:Cldn1 protein, mouse; D008565:Membrane Proteins