Naturally occurring anti-Escherichia coli protein antibodies in the sera of healthy humans cause analytical interference in a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay for serodiagnosis of severe acute respiratory syndrome.
We reported the analytical interference of anti-Escherichia coli protein (EP) antibodies in human sera and residual EP in a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay as a possible source of false positives in severe acute respiratory syndrome serodiagnosis. The rate of false positives was significantly reduced by adding mouse anti-EP antiserum in the blocking step.
Yip
Chi Wai
CW
Department of Zoology, Kadoorie Biological Science Building, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, China.
Hon
Chung Chau
CC
Zeng
Fanya
F
Chow
Ken Y C
KY
Chan
Kwok Hung
KH
Peiris
Joseph S M
JS
Leung
Frederick C C
FC
D000907:Antibodies, Bacterial / Q000276:immunology; D000086462:Coronavirus Nucleocapsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D029968:Escherichia coli Proteins / Q000276:immunology*; D006801:Humans; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology / Q000302:isolation & purification*; D012698:Serologic Tests / Q000379:methods; D045169:Severe Acute Respiratory Syndrome / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000907:Antibodies, Bacterial; D000086462:Coronavirus Nucleocapsid Proteins; D029968:Escherichia coli Proteins; D019590:Nucleocapsid Proteins