title: Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L.
abstract: A transcription factor RCBF2 which interacts with C-repeat/DRE was isolated from Oryza sativa L. by a yeast one-hybrid method. Analysis of the deduced RCBF2 amino acid sequence revealed that RCBF2 contained a conserved ethylene-responsive element binding protein (EREBP)/AP2 domain of 59 amino acids and a potential nuclear localization sequence. RCBF2 showed a high level of homology with other CBF family members only in AP2 domain. Phylogenetic analysis showed that RCBF2 might be different from other eight DRE-binding proteins on evolutionary relationship. The semi-quantitative RT-PCR (s-Q RT-PCR) analysis indicated the expression of RCBF2 gene was induced by cold, dehydration and high-salinity, but not by abscisic acid, and the transcription of RCBF2 gene accumulated primarily in rice immature seeds, growing point and shoots.
authors.affiliation: Department of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, People's Republic of China.
authors.forename: Jin-Ge
authors.initials: JG
authors.lastname: Liu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qiu-Lin
authors.initials: QL
authors.lastname: Qin
authors.forename: Ri-He
authors.initials: RH
authors.lastname: Peng
authors.forename: Ai-Sheng
authors.initials: AS
authors.lastname: Xiong
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Chen
authors.forename: Fang
authors.initials: F
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
authors.forename: Quan-Hong
authors.initials: QH
authors.lastname: Yao
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D010940:Plant Proteins; C083115:activator protein-2 binding element; C093680:ethylene-responsive element binding protein