title: High-throughput measurements of biochemical responses using the plate::vision multimode 96 minilens array reader.
abstract: The plate::vision is a high-throughput multimode reader capable of reading absorbance, fluorescence, fluorescence polarization, time-resolved fluorescence, and luminescence. Its performance has been shown to be quite comparable with other readers. When the reader is integrated into the plate::explorer, an ultrahigh-throughput screening system with event-driven software and parallel plate-handling devices, it becomes possible to run complicated assays with kinetic readouts in high-density microtiter plate formats for high-throughput screening. For the past 5 years, we have used the plate::vision and the plate::explorer to run screens and have generated more than 30 million data points. Their throughput, performance, and robustness have speeded up our drug discovery process greatly.
authors.affiliation: Research Discovery Technologies, Hoffmann-La Roche Inc., Nutley, NJ, USA.
authors.forename: Kuo-Sen
authors.initials: KS
authors.lastname: Huang
authors.forename: David
authors.initials: D
authors.lastname: Mark
authors.forename: Frank Ulrich
authors.initials: FU
authors.lastname: Gandenberger
mesh_terms: D000042:Absorption; D000251:Adenosine Triphosphatases / Q000737:chemistry; D001331:Automation; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D020650:Combinatorial Chemistry Techniques; D015195:Drug Design; D004345:Drug Industry / Q000639:trends; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D008956:Models, Chemical; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000251:Adenosine Triphosphatases