Performance of a commercial immunoglobulin M antibody capture assay using analyte-specific reagents to screen for interfering factors during a West Nile virus epidemic season in Nebraska.
In 2003, the Nebraska Public Health Laboratory tested more than 10,371 serum and 516 cerebral spinal fluid specimens. Results showed that without performing the interfering factors screen for specimens in the low positive index value range of >1.1 to <or=3.5, a false positivity rate of 6.5% would have been realized.
Sambol
Anthony R
AR
Nebraska Public Health Laboratory, University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, NE 68198-6495, USA. asambol@unmc.edu
Hinrichs
Steven H
SH
Hogrefe
Wayne R
WR
Schweitzer
Beth K
BK
D000914:Antibodies, Viral / Q000097:blood*; D007075:Immunoglobulin M; D009329:Nebraska; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D012189:Retrospective Studies; D012698:Serologic Tests; D014762:Viral Interference / Q000276:immunology*; D014901:West Nile Fever / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D014902:West Nile virus / Q000276:immunology*
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic