Carbon dioxide and pH effects on temperature-sensitive and -insensitive hypothalamic neurons.
The preoptic-anterior hypothalamus (POAH) controls body temperature, and thermoregulatory responses are impaired during hypercapnia. If increased CO(2) or its accompanying acidosis inhibits warm-sensitive POAH neurons, this could provide an explanation for thermoregulatory impairment during hypercapnia. To test this possibility, extracellular electrophysiological recordings determined the effects of CO(2) and pH on the firing rates of both temperature-sensitive and -insensitive neurons in hypothalamic tissue slices from 89 male Sprague-Dawley rats. Firing rate activity was recorded in 121 hypothalamic neurons before, during, and after changing the CO(2) concentration aerating the tissue slice chamber or changing the pH of the solution bathing the tissue slices. Increasing the aeration CO(2) concentration from 5% (control) to 10% (hypercapnic) had no effect on most (i.e., 69%) POAH temperature-insensitive neurons; however, this hypercapnia inhibited the majority (i.e., 59%) of warm-sensitive neurons. CO(2) affected similar proportions of (non-POAH) neurons in other hypothalamic regions. These CO(2) effects appear to be due to changes in pH since the CO(2)-affected neurons responded similarly to isocapnic acidosis (i.e., normal CO(2) and decreased pH) but were not responsive to isohydric hypercapnia (i.e., increased CO(2) and normal pH). These findings may offer a neural explanation for some heat-related illnesses (e.g., exertional heat stroke) where impaired heat loss is associated with acidosis.
Wright
Chadwick L
CL
Department of Physiology and Cell Biology, 201 Hamilton Hall, Ohio State University, 1645 Neil Ave., Columbus, OH 43210, USA.
Boulant
Jack A
JA
D000138:Acidosis / Q000150:complications / Q000503:physiopathology*; D000818:Animals; D001833:Body Temperature Regulation; D002245:Carbon Dioxide / Q000378:metabolism*; D002478:Cells, Cultured; D018883:Heat Stroke / Q000209:etiology / Q000503:physiopathology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006935:Hypercapnia / Q000150:complications / Q000503:physiopathology*; D007031:Hypothalamus / Q000503:physiopathology*; D008297:Male; D009433:Neural Inhibition; D009475:Neurons, Afferent / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002245:Carbon Dioxide