A novel methodology for imaging new bone formation around bioceramic bone substitutes.
In this study, Frost's bulk-staining in combination with confocal laser scanning microscopy (CLSM) was used to image and characterize ground sections of undecalcified rat bone tissue with in situ bioceramic implants. This was addressed by bulk staining specimens in alcohol-soluble basic fuchsin dye. The ground sections were imaged using CLSM in the confocal fluorescence mode. Confocal images revealed that the newly formed bone could be clearly distinguished from bone marrow and cortical bone, as well as from the implant material.
Gorustovich
Alejandro A
AA
Research Laboratory, National Atomic Energy Commission (CNEA-Regional Noroeste), A4408FTV Salta, Argentina. alegorustov@ciudad.com.ar
Sivak
Matías G
MG
Guglielmotti
María B
MB
D000818:Animals; D018786:Bone Substitutes; D001842:Bone and Bones / Q000473:pathology* / Q000601:surgery*; D002516:Ceramics; D005898:Glass; D008297:Male; D008422:Materials Testing; D018613:Microscopy, Confocal; D016348:Osseointegration; D051381:Rats; D017208:Rats, Wistar; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods
D018786:Bone Substitutes; D012394:Rosaniline Dyes; C425330:bioactive glass 45S5; C025485:basic fuchsin