HLA class I epitope discovery in type 1 diabetes: independent and reproducible identification of proinsulin epitopes of CD8 T cells--report of the IDS T Cell Workshop Committee.
Islet autoreactive CD8 T cells are plausible candidates for direct beta cell toxicity in type 1 diabetes (T1DM). In 2005, cellular studies in the pathogenesis of this disease have reached a new milestone. Autoreactive CD8 T cells have been defined and several target islet epitopes of these have been discovered and validated simultaneously in three independent studies. The insulin B10-B18 peptide that displays exceptional binding affinity for HLA-A2 has been reported in all three studies, and its recognition shows an association with autoimmune beta cell destruction and T1DM. These studies imply that CD8 T cell-based HLA tetramers and ELISPOT analyses can be useful to monitor T1DM as well as islet transplantation, and may provide useful tools to assess immunological efficacy of immune intervention trials.
Pinkse
Gabrielle G M
GG
Department of Immunohematology and Blood Transfusion, E3Q, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
Boitard
Christian
C
Tree
Timothy I M
TI
Peakman
Mark
M
Roep
Bart O
BO
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology* / Q000473:pathology; D000939:Epitopes / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology / Q000473:pathology; D051379:Mice; D011384:Proinsulin / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015203:Reproducibility of Results
D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D011384:Proinsulin