title: Poly-(3-hexylthiophene) self-assembled monolayer based cholesterol biosensor using surface plasmon resonance technique.
abstract: Cholesterol oxidase (ChOx) has been covalently immobilized onto 1-fluoro-2-nitro-4-azidobenzene (FNAB) modified poly-(3-hexylthiophene) (P3HT) self-assembled monolayer (SAM) onto gold coated glass plates. These ChOx/FNAB/P3HT/Au bio-electrodes have been characterized using contact angle (CA) measurements, UV-vis spectroscopy, electrochemical impedance technique, cyclic voltammetric technique and atomic force microscopic (AFM) technique, respectively. The ChOx/FNAB/P3HT/Au bio-electrodes were utilized for the estimation of cholesterol concentration in standard solutions using surface plasmon resonance (SPR) technique. It is shown that this SPR biosensor has linearity from 50 to 500 mg/dl of cholesterol in solution with detection limit of 50 mg/dl, sensitivity of 1.0 4 m degrees /(mg dl), reusability of around 15 times and a shelf-life of about 10 weeks when stored at 4 degrees C.
authors.affiliation: Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi, India.
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Arya
authors.forename: Pratima R
authors.initials: PR
authors.lastname: Solanki
authors.forename: S P
authors.initials: SP
authors.lastname: Singh
authors.forename: K
authors.initials: K
authors.lastname: Kaneto
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Pandey
authors.forename: Monika
authors.initials: M
authors.lastname: Datta
authors.forename: Bansi D
authors.initials: BD
authors.lastname: Malhotra
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002784:Cholesterol / Q000032:analysis* / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008567:Membranes, Artificial; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods; D013499:Surface Properties; D013876:Thiophenes / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D013876:Thiophenes; C507295:poly(3-hexylthiophene); D002784:Cholesterol