Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens.
Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitivities and specificities.
Nantavisai
Kwannan
K
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Mungthin
Mathirut
M
Tan-ariya
Peerapan
P
Rangsin
Ram
R
Naaglor
Tawee
T
Leelayoova
Saovanee
S
D000818:Animals; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005374:Filtration / Q000379:methods; D005872:Giardia / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D005873:Giardiasis / Q000469:parasitology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012335:RNA, Ribosomal / Q000235:genetics; D012680:Sensitivity and Specificity
D017931:DNA Primers; D016054:DNA, Protozoan; D012335:RNA, Ribosomal