A cytokinin perception mutant colonized by Rhizobium in the absence of nodule organogenesis.
In legumes, Nod-factor signaling by rhizobia initiates the development of the nitrogen-fixing nodule symbiosis, but the direct cell division stimulus that brings about nodule primordia inception in the root cortex remains obscure. We showed that Lotus japonicus plants homozygous for a mutation in the HYPERINFECTED 1 (HIT1) locus exhibit abundant infection-thread formation but fail to initiate timely cortical cell divisions in response to rhizobial signaling. We demonstrated that the corresponding gene encodes a cytokinin receptor that is required for the activation of the nodule inception regulator Nin and nodule organogenesis.
Murray
Jeremy D
JD
Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford Street, London, Ontario N5V 4T3, Canada.
Karas
Bogumil J
BJ
Sato
Shusei
S
Tabata
Satoshi
S
Amyot
Lisa
L
Szczyglowski
Krzysztof
K
D000483:Alleles; D020561:Alphaproteobacteria / Q000254:growth & development / Q000502:physiology*; D002455:Cell Division; D003583:Cytokinins / Q000378:metabolism* / Q000494:pharmacology; D017343:Genes, Plant; D000071677:Histidine Kinase; D008070:Lipopolysaccharides / Q000378:metabolism; D000070116:Lotus / Q000235:genetics / Q000382:microbiology* / Q000502:physiology*; D009154:Mutation; D009586:Nitrogen Fixation; D019441:Plant Epidermis / Q000166:cytology / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D053204:Root Nodules, Plant / Q000166:cytology / Q000254:growth & development* / Q000382:microbiology; D015398:Signal Transduction; D013559:Symbiosis
D003583:Cytokinins; D008070:Lipopolysaccharides; C403406:NIN protein, Lotus japonicus; D010940:Plant Proteins; D011956:Receptors, Cell Surface; D011494:Protein Kinases; D000071677:Histidine Kinase