Determination of glucuronidated 5-hydroxytryptophol (GTOL), a marker of recent alcohol intake, by ELISA technique.
OBJECTIVE
To compare markers of alcohol consumption.


METHODS
Measurement of urinary ethyl glucuronide, 5-hydroxytryptophol, 5-hydroxytryptophol glucuronide, 5-hydroxyindole acetic acid in 10 patients during alcohol withdrawal.


RESULTS
5-Hydroxytryptophol glucuronide was measured by ELISA with good analytical precision, its diagnostic specificity and sensitivity was better than that of 5-hydroxytryptophol and its correlation was closer to ethyl glucuronide than to 5-hydroxytryptophol.


CONCLUSIONS
Determination of 5-hydroxytryptophol glucuronide by ELISA offers promising results in detection of previous alcohol consumption.
Dierkes
Jutta
J
Institute of Clinical Chemistry, Otto von Guericke University, Magdeburg, Germany.
Wolfersdorf
Manfred
M
Borucki
Katrin
K
Weinmann
Wolfgang
W
Wiesbeck
Gerhard
G
Beck
Olof
O
Borg
Stefan
S
Wurst
Friedrich M
FM
D000328:Adult; D000428:Alcohol Drinking / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D020719:Glucuronides / Q000032:analysis*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D006917:Hydroxytryptophol / Q000031:analogs & derivatives* / Q000032:analysis*; D008297:Male; D013375:Substance Withdrawal Syndrome
C500750:5-hydroxytryptophol glucuronide; D015415:Biomarkers; D020719:Glucuronides; D006917:Hydroxytryptophol; D006897:Hydroxyindoleacetic Acid