The Arabidopsis histidine phosphotransfer proteins are redundant positive regulators of cytokinin signaling.
Arabidopsis thaliana histidine phosphotransfer proteins (AHPs) are similar to bacterial and yeast histidine phosphotransfer proteins (HPts), which act in multistep phosphorelay signaling pathways. A phosphorelay pathway is the current model for cytokinin signaling. To assess the role of AHPs in cytokinin signaling, we isolated T-DNA insertions in the five AHP genes that are predicted to encode functional HPts and constructed multiple insertion mutants, including an ahp1,2,3,4,5 quintuple mutant. Single ahp mutants were indistinguishable from wild-type seedlings in cytokinin response assays. However, various higher-order mutants displayed reduced sensitivity to cytokinin in diverse cytokinin assays, indicating both a positive role for AHPs in cytokinin signaling and functional overlap among the AHPs. In contrast with the other four AHPs, AHP4 may play a negative role in some cytokinin responses. The quintuple ahp mutant showed various abnormalities in growth and development, including reduced fertility, increased seed size, reduced vascular development, and a shortened primary root. These data indicate that most of the AHPs are redundant, positive regulators of cytokinin signaling and affect multiple aspects of plant development.
Hutchison
Claire E
CE
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280, USA.
Li
Jie
J
Argueso
Cristiana
C
Gonzalez
Monica
M
Lee
Eurie
E
Lewis
Michael W
MW
Maxwell
Bridey B
BB
Perdue
Tony D
TD
Schaller
G Eric
GE
Alonso
Jose M
JM
Ecker
Joseph R
JR
Kieber
Joseph J
JJ
D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D003583:Cytokinins / Q000378:metabolism* / Q000494:pharmacology; D004269:DNA, Bacterial / Q000378:metabolism; D006639:Histidine / Q000378:metabolism*; D018546:Hypocotyl / Q000166:cytology / Q000187:drug effects; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010770:Phosphotransferases / Q000378:metabolism*; D010802:Phylogeny; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects; D018520:Plant Shoots / Q000166:cytology / Q000187:drug effects; D036226:Seedlings / Q000166:cytology / Q000187:drug effects; D012639:Seeds / Q000166:cytology / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects
D029681:Arabidopsis Proteins; D003583:Cytokinins; D004269:DNA, Bacterial; C036483:T-DNA; D002734:Chlorophyll; D006639:Histidine; D010770:Phosphotransferases