Gene delivery by combination of novel liposomal bubbles with perfluoropropane and ultrasound.
Microbubbles and ultrasound have recently been investigated with a view to improving the transfection efficiency of non-viral gene delivery systems. However, microbubbles are unstable and their targeting ability is insufficient for clinical use. To circumvent these problems, we developed novel polyethyleneglycol (PEG) modified liposomes (Bubble liposomes) containing perfluoropropane, which is an ultrasound imaging gas. Here, we used ultrasound to induce cavitation in Bubble liposomes and then investigated their ability to deliver genes in vitro and in vivo. Bubble liposomes could deliver plasmid DNA to many cell types without cytotoxicity. Additionally, in vivo gene delivery, Bubble liposomes were more effective delivery into femoral artery than lipofection method. Thus, Bubble liposomes might be efficient and novel non-viral tools for gene delivery.
Suzuki
Ryo
R
Department of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University, 1091-1 Suwarashi, Sagamiko-cho, Sagamihara, Kanagawa, Japan.
Takizawa
Tomoko
T
Negishi
Yoichi
Y
Hagisawa
Kosuke
K
Tanaka
Kumiko
K
Sawamura
Kaori
K
Utoguchi
Naoki
N
Nishioka
Toshihiko
T
Maruyama
Kazuo
K
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D005079:Excipients; D005347:Fibroblasts; D005466:Fluorocarbons; D018014:Gene Transfer Techniques; D008081:Liposomes / Q000737:chemistry*; D008156:Luciferases / Q000235:genetics; D051379:Mice; D010957:Plasmids / Q000235:genetics; D014162:Transfection; D014465:Ultrasonics
D005079:Excipients; D005466:Fluorocarbons; D008081:Liposomes; D004247:DNA; C042852:perflutren; D008156:Luciferases