title: Fast manipulation of cellular cAMP level by light in vivo.
abstract: The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACalpha and PACbeta. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells.
authors.affiliation: Max-Planck-Institut für Biophysik, 60439 Frankfurt, Germany.
authors.forename: Saskia
authors.initials: S
authors.lastname: Schröder-Lang
authors.forename: Martin
authors.initials: M
authors.lastname: Schwärzel
authors.forename: Reinhard
authors.initials: R
authors.lastname: Seifert
authors.forename: Timo
authors.initials: T
authors.lastname: Strünker
authors.forename: Suneel
authors.initials: S
authors.lastname: Kateriya
authors.forename: Jens
authors.initials: J
authors.lastname: Looser
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Watanabe
authors.forename: U Benjamin
authors.initials: UB
authors.lastname: Kaupp
authors.forename: Peter
authors.initials: P
authors.lastname: Hegemann
authors.forename: Georg
authors.initials: G
authors.lastname: Nagel
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D000242:Cyclic AMP / Q000032:analysis / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D004789:Enzyme Activation / Q000528:radiation effects; D005056:Euglena gracilis / Q000201:enzymology; D018014:Gene Transfer Techniques; D006801:Humans; D008027:Light; D013997:Time Factors; D014982:Xenopus laevis
chemical_list: D000242:Cyclic AMP; D000262:Adenylyl Cyclases