Interaction of N-linked glycans, having multivalent GlcNAc termini, with GM3 ganglioside.
GM3 ganglioside interacts specifically with complex-type N-linked glycans having multivalent GlcNAc termini, as shown for (1) and (2) below. (1) Oligosaccharides (OS) isolated from ConA-non-binding N-linked glycans of ovalbumin, whose structures were identified as penta-antennary complex-type with bisecting GlcNAc, having five or six GlcNAc termini (OS B1, B2), or bi-antennary complex-type having two GlcNAc termini (OS I). OS I is a structure not previously described. (2) Multi-antennary complex-type N-linked OS isolated from fetuin, treated by sialidase followed by beta-galactosidase, having three or four GlcNAc termini exposed. These OS, conjugated to phosphatidylethanolamine (PE), showed clear interaction with (3)H-labeled liposomes containing GM3, when various doses of OS-PE conjugate were adhered by drying to multi-well polystyrene plates. Interaction was clearly observed only with liposomes containing GM3, but not LacCer, Gb4, or GalNAcalpha1-3Gb4 (Forssman antigen). GM3 interaction with PE conjugate of OS B1 or B2 was stronger than that with PE conjugate of OS I. GM3 interacted clearly with PE conjugate of N-linked OS from desialylated and degalactosylated fetuin, but not native fetuin. No binding was observed to cellobiose-PE conjugate, or to OS-PE conjugate lacking GlcNAc terminus. Thus, GM3, but not other GSL liposomes, interacts with various N-linked OS having multiple GlcNAc termini, in general. These findings suggest that the concept of carbohydrate-to-carbohydrate interaction can be extended to interaction of specific types of N-linked glycans with specific GSLs. Natural occurrence of such interaction to define cell biological phenomena is under investigation.
Yoon
Seon-Joo
SJ
Division of Biomembrane Research, Pacific Northwest Research Institute, and Department of Pathobiology, University of Washington, Seattle, WA, USA.
Nakayama
Ken-Ichi
K
Takahashi
Noriko
N
Yagi
Hirokazu
H
Utkina
Natalia
N
Wang
Helen Ying
HY
Kato
Koichi
K
Sadilek
Martin
M
Hakomori
Sen-itiroh
SI
D000818:Animals; D002240:Carbohydrate Sequence; D005679:G(M3) Ganglioside / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D008081:Liposomes; D009439:Neuraminidase / Q000494:pharmacology; D010047:Ovalbumin / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000737:chemistry; D001616:beta-Galactosidase / Q000494:pharmacology
D005679:G(M3) Ganglioside; D006023:Glycoproteins; D008081:Liposomes; D010714:Phosphatidylethanolamines; D011134:Polysaccharides; D000509:alpha-Fetoproteins; C483858:phosphatidylethanolamine; D010047:Ovalbumin; D009439:Neuraminidase; D001616:beta-Galactosidase