title: Influence of haemodialysis and left ventricular failure on peripheral A(2A) adenosine receptor expression.
abstract: BACKGROUND
  Haemodialysis (HD) sometimes accelerates left ventricular failure (LVF). As adenosine (ADO) is strongly implicated in cardiovascular functions, particularly via A(2A) receptor activation and as changes of peripheral A(2A) receptors mirror changes occurring in the cardiovascular system, we examined the influence of HD and LVF on both ADO plasma concentration and the expression of A(2A) receptors (i.e. Bmax, K(D) and mRNA amount) of peripheral blood mononuclear cells.
  
  
  METHODS
  This cross-sectional study included 61 chronic renal failure (CRF) patients: 41 without LVF (24 haemodialysed and 17 undialysed) and 20 with LVF (9 haemodialysed and 11 undialysed). Ten LVF patients without CRF and 10 healthy subjects were also examined.
  
  
  RESULTS
  (i) Bmax values of CRF patients without LVF were significantly decreased in undialysed patients compared with haemodialysed patients, and compared with controls (69 +/- 25 vs 98 +/- 33 vs 180 +/- 60 fmol/mg of protein, P < 0.05). Bmax values of CRF patients with LVF were lower in undialysed patients than in haemodialysed patients (60 +/- 27 vs 101 +/- 27 fmol/mg of protein, P < 0.05). Bmax values of LVF patients without CRF were lower than in controls (51 +/- 19 vs 180 +/- 60 fmol/mg of protein). (ii) A(2A) mRNA expression was increased in haemodialysed patients compared with controls (20.2 +/- 0.75 vs 17.6 +/- 1.3, P < 0.05). (iii) ADO plasma levels were high in haemodialysed patients and further increased during the HD sessions.
  
  
  CONCLUSIONS
  The number of A(2A) receptors was decreased by CRF with or without LVF. However, this decrease was less important in haemodialysed patients. The changes in peripheral A(2A) receptor expression suggest a significant inflammatory response to HD and heart or kidney failure. Whether these changes do reflect alterations in cardiomyocytes needs further investigation.
authors.affiliation: FRE2738 CNRS/Université de la Méditerranée, Faculté de Médecine Nord, Bd P. Dramard, 13015 Marseille, France.
authors.forename: Louis
authors.initials: L
authors.lastname: Carrega
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Fenouillet
authors.forename: Philippe
authors.initials: P
authors.lastname: Giaime
authors.forename: Annie
authors.initials: A
authors.lastname: Charavil
authors.forename: Laurence
authors.initials: L
authors.lastname: Mercier
authors.forename: Victoria
authors.initials: V
authors.lastname: Gerolami
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Berge-Lefranc
authors.forename: Yvon
authors.initials: Y
authors.lastname: Berland
authors.forename: Jean
authors.initials: J
authors.lastname: Ruf
authors.forename: Alain
authors.initials: A
authors.lastname: Saadjian
authors.forename: Bertrand
authors.initials: B
authors.lastname: Dussol
authors.forename: Regis
authors.initials: R
authors.lastname: Guieu
mesh_terms: D000241:Adenosine / Q000097:blood; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D001806:Blood Urea Nitrogen; D002845:Chromatography; D003404:Creatinine / Q000097:blood; D003430:Cross-Sectional Studies; D005260:Female; D005612:Freeze Drying; D015870:Gene Expression; D006333:Heart Failure / Q000209:etiology* / Q000235:genetics / Q000503:physiopathology; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000235:genetics; D007676:Kidney Failure, Chronic / Q000097:blood / Q000150:complications / Q000628:therapy*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics*; D043705:Receptor, Adenosine A2A / Q000097:blood / Q000235:genetics*; D006435:Renal Dialysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index; D013318:Stroke Volume / Q000502:physiology; D018487:Ventricular Dysfunction, Left / Q000150:complications* / Q000235:genetics / Q000503:physiopathology
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D043705:Receptor, Adenosine A2A; D003404:Creatinine; D000241:Adenosine