title: Cellular Localization of Inhibin alpha-subunit, PKB/Akt and FoxO3a proteins in the ovaries of minipigs.
abstract: Experiments were conducted to examine the cellular localization of inhibin alpha-subunit, protein kinase B (PKB/Akt), and FoxO3a proteins in the ovaries of minipigs, Chinese Xiang pigs, by immunohistochemistry. The results indicated that inhibin alpha-subunits were localized in the granulosa cells of follicles at all stages but were not localized in corpora lutea. PKB was localized in the granulosa cells of primordial follicles and in the basal layers of the granulosa cells of preantral and antral follicles, but were not localized in atretic follicles and corpora lutea. FoxO3a was localized in the granulosa cells of follicles at all stages and was extensively localized in the cytoplasma of the luteinized granulosa cells of corpora lutea. Together, the stage- and cell-specific expression patterns of inhibin alpha-subunit, FoxO3a, and PKB suggest that these proteins might play potential roles in follicular development, atresia, and luteinization in the minipig.
authors.affiliation: Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Meng
authors.forename: Fangxiong
authors.initials: F
authors.lastname: Shi
authors.forename: Zhenqi
authors.initials: Z
authors.lastname: Zhou
authors.forename: Ruihua
authors.initials: R
authors.lastname: Huang
authors.forename: Gentao
authors.initials: G
authors.lastname: Liu
authors.forename: Gen
authors.initials: G
authors.lastname: Watanabe
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Taya
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D006107:Granulosa Cells / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007265:Inhibins / Q000378:metabolism*; D010053:Ovary / Q000166:cytology* / Q000502:physiology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D013552:Swine; D013556:Swine, Miniature
chemical_list: D051858:Forkhead Transcription Factors; C094377:inhibin-alpha subunit; D007265:Inhibins; D051057:Proto-Oncogene Proteins c-akt