Cytokines, neurotrophins, and oxidative stress in brain disease from mucopolysaccharidosis IIIB.
Mucopolysaccharidosis IIIB (MPS IIIB; Sanfilippo syndrome type B) is characterized by profound neurological deterioration. Because a murine model of MPS IIIB disease is available, we focused on analysis of gene expression in the brain and cerebellum of 7-month-old MPS IIIB mice by pathway-specific filter microarrays designed to probe apoptotic-related, neurotrophic signalling molecules and inflammatory cytokines and receptors. Moreover, we extended the analysis with real-time PCR performed at 1, 3, 7 months after birth. Bdnf was down-regulated in the brain but up-regulated in the cerebellum at 7 months of age, both at RNA and at protein levels. Cbln1 presented a threefold increase in the oldest brains while remaining unaltered in the cerebellum. Ccl3, Casp11, gp91(phox), p67(phox), and p47(phox) showed an increased expression in both brain and cerebellum at each examined time point. Ccl3, in particular, exhibited in both organs and at all times tested approximately a tenfold increase in its expression. Insofar as p47(phox), p67(phox), and gp91(phox) are all components of the phagocyte NADPH oxidase, our results suggest the possible involvement of the reactive oxygen species in the genesis of neurodegeneration in MPS IIIB disease.
Villani
Guglielmo R D
GR
Department of Biochemistry and Medical Biotechnologies, University of Naples Federico II, Naples, Italy. villani@dbbm.unina.it
Gargiulo
Nadia
N
Faraonio
Raffaella
R
Castaldo
Sigismondo
S
Gonzalez Y Reyero
Enrico
E
Di Natale
Paola
P
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D001921:Brain / Q000473:pathology / Q000503:physiopathology; D001927:Brain Diseases / Q000235:genetics* / Q000503:physiopathology; D016207:Cytokines / Q000235:genetics*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D051379:Mice; D009084:Mucopolysaccharidosis III / Q000235:genetics* / Q000503:physiopathology; D019255:NADPH Oxidases / Q000378:metabolism; D009414:Nerve Growth Factors / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016207:Cytokines; D009414:Nerve Growth Factors; D012313:RNA; D019255:NADPH Oxidases