Molecular authentication of Alisma orientale by PCR-RFLP and ARMS.
As a widely used medicinal plant, Alisma orientale is always a possible target for fraudulent labeling. The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (nrDNA) of the six species of genus Alisma were sequenced, and two variant sites were found to be specific for A. orientale. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis and amplification refractory mutation system (ARMS) analysis were applied to the ITS region for the identification of A. orientale. A restriction site for PSTI useful for PCR-RFLP analysis was detected and a pair of diagnostic primers DFZX-JB02S and DFZX-JB02X were designed for ARMS.
Li
Xuexia
X
Jiangsu Key Laboratory for Biodiversity & Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, P. R. China.
Ding
Xiaoyu
X
Chu
Bihai
B
Ding
Ge
G
Gu
Sun
S
Qian
Liang
L
Wang
Ying
Y
Zhou
Qi
Q
D034902:Alisma / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis*; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D006801:Humans; D008517:Phytotherapy; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D017931:DNA Primers; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer