Identification and analysis of novel glycolipids in vertebrate brains by HPLC/mass spectrometry.
Glycosphingolipids are a major component of microdomains or lipid rafts in biological membranes. A new member of raft glycolipids, phosphatidylglucoside (PtdGlc), as well as 6-O-Ac-PtdGlc, a form of PtdGlc O-acetylated at position 6 of its glucopyranose ring, is present in central nervous system tissues. Because the glycolipids represent a minor constituent of lipid rafts and because their mass numbers are the same as that of phosphatidylinositol (PI), the glycolipids are difficult to detect and purify. Here we describe methods to purify and identify glycolipids from rodent brain and methods to discriminate PtdGlc from PI in chick spinal cord using HPLC/electrospray ionization ion-trap mass spectrometry.
Nagatsuka
Yasuko
Y
Hirabayashi Laboratory Unit and CREST, Neuronal Circuit Mechanisms Research Group, Brain Science Institute RIKEN, Saitama, Japan.
Tojo
Hiromasa
H
Hirabayashi
Yoshio
Y
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D020404:Glycerophospholipids / Q000737:chemistry; D006017:Glycolipids / Q000032:analysis* / Q000737:chemistry*; D010716:Phosphatidylinositols / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D020404:Glycerophospholipids; D006017:Glycolipids; D010716:Phosphatidylinositols; C429823:phosphatidylglucose