title: High-throughput analysis of mumps virus and the virus-specific monoclonal antibody on the arrays of a cationic polyelectrolyte with a spectral SPR biosensor.
abstract: We investigated the potential use of a spectral surface plasmon resonance (SPR) biosensor in a high-throughput analysis of mumps virus and a mumps virus-specific mAb on the arrays of a cationic polyelectrolyte, poly(diallyldimethylammonium chloride) (PDDA). The PDDA surface was constructed by electrostatic adsorption of the polyelectrolyte onto a monolayer of 11-mercaptoundecanoic acid (MUA). Poly-L-lysine was also adsorbed onto the MUA monolayer and compared with the PDDA surface in the capacity of mumps virus immobilization. The PDDA surface showed a higher adsorption of mumps virus than the poly-L-lysine surface. The SPR signal caused by the virus binding onto the PDDA surface was proportional to the concentration of mumps virus from 0.5 x 10(5) to 14 x 10(5) pfu/mL. The surface structure of the virus arrays was visualized by atomic force microscopy. Then, a dose-dependent increase in the SPR signal was observed when various concentrations of the antimumps virus antibody in buffer or human serum were applied to the virus arrays, and their interaction was specific. Thus, it is likely that the spectral SPR biosensor based on the cationic polyelectrolyte surface may provide an efficient system for a high-throughput analysis of intact virus and serodiagnosis of infectious diseases.
authors.affiliation: Department of Molecular and Cellular Biochemistry, Kangwon National University College of Medicine, Chuncheon, Kangwon-do, Korea.
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Se-Hui
authors.initials: SH
authors.lastname: Jung
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: In-Bum
authors.initials: IB
authors.lastname: Suh
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Kim
authors.forename: Jae-Wan
authors.initials: JW
authors.lastname: Jung
authors.forename: Jong Seol
authors.initials: JS
authors.lastname: Yuk
authors.forename: Young-Myeong
authors.initials: YM
authors.lastname: Kim
authors.forename: Kwon-Soo
authors.initials: KS
authors.lastname: Ha
mesh_terms: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood; D006801:Humans; D018625:Microscopy, Atomic Force; D009107:Mumps / Q000175:diagnosis / Q000276:immunology; D009109:Mumps virus / Q000276:immunology* / Q000302:isolation & purification*; D011073:Polyamines; D000071228:Polyelectrolytes; D011095:Polyethylenes; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods; D000644:Quaternary Ammonium Compounds; D012698:Serologic Tests; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D011073:Polyamines; D000071228:Polyelectrolytes; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride