A cleavable amino-thiol linker for reversible linking of amines to DNA.
A cleavable heterobifunctional cross-linker for the reversible conjugation of amines to thiol-modified DNA has been developed and tested. The succinimidyl 2-(vinylsulfonyl)ethyl carbonate (SVEC) was prepared in three steps and tested for its ability to react with amines and thiols. The linker was efficient for binding leucine to a thiol-modified DNA sequence and for releasing the amino acid at pH 11.8.
Højfeldt
Jonas W
JW
Center for Catalysis and Interdisciplinary Nanoscience Center (iNANO), Department of Chemistry, University of Aarhus, Langelandsgade 140, 8000 Aarhus C, Denmark.
Blakskjaer
Peter
P
Gothelf
Kurt V
KV
D000588:Amines / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D000588:Amines; D013438:Sulfhydryl Compounds; D004247:DNA