title: Multivariate analysis approach to the plasma protein profile of patients with advanced colorectal cancer.
abstract: The aim of the present study was to identify the pattern of plasma protein species of interest as markers of colorectal cancer (CRC). Using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), the plasma protein profile was determined in nine stage IV CRC patients (study group) and nine clean-colon healthy subjects (control group). Multivariate analysis methods were employed to identify distinctive disease patterns at protein spectrum. In the study and control groups, cluster analysis (CA) on the complete MALDI-MS spectra plasma protein profile showed a distinction between CRC patients and healthy subjects, thus allowing the identification of the most discriminating ionic species. Principal component analysis (PCA) and linear discriminant analysis (LDA) yielded similar grouping results. LDA with leave-one-out cross validation achieved a correct classification rate of 89% in both the patients and the healthy subjects.
authors.affiliation: Department of Pharmacology and Anesthesiology, University of Padova, Italy.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Ragazzi
authors.forename: Salvatore
authors.initials: S
authors.lastname: Pucciarelli
authors.forename: Roberta
authors.initials: R
authors.lastname: Seraglia
authors.forename: Laura
authors.initials: L
authors.lastname: Molin
authors.forename: Marco
authors.initials: M
authors.lastname: Agostini
authors.forename: Mario
authors.initials: M
authors.lastname: Lise
authors.forename: Pietro
authors.initials: P
authors.lastname: Traldi
authors.forename: Donato
authors.initials: D
authors.lastname: Nitti
mesh_terms: D000230:Adenocarcinoma / Q000097:blood*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D016000:Cluster Analysis; D015179:Colorectal Neoplasms / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D015999:Multivariate Analysis; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins