In vivo photoacoustic flow cytometry for monitoring of circulating single cancer cells and contrast agents.
A new photoacoustic flow cytometry was developed for real-time detection of circulating cells, nanoparticles, and contrast agents in vivo. Its capability, integrated with photothermal and optical clearing methods, was demonstrated using a near-infrared tunable laser to characterize the in vivo kinetics of Indocyanine Green alone and single cancer cells labeled with gold nanorods and Indocyanine Green in the vasculature of the mouse ear. In vivo applications are discussed, including selective nanophotothermolysis of metastatic squamous cells, label-free detection of melanoma cells, study of pharmokinetics, and immune response to apoptotic and necrotic cells, with potential translation to humans. The threshold sensitivity is estimated as one cancer cell in the background of 10(7) normal blood cells.
Zharov
Vladimir P
VP
University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA. zharovvladimirp@uams.edu
Galanzha
Ekaterina I
EI
Shashkov
Evgeny V
EV
Khlebtsov
Nicolai G
NG
Tuchin
Valery V
VV
D000162:Acoustics / Q000295:instrumentation*; D000818:Animals; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D003287:Contrast Media; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D005456:Fluorescent Dyes; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D009369:Neoplasms / Q000473:pathology*; D014463:Ultrasonography / Q000295:instrumentation* / Q000379:methods
D003287:Contrast Media; D005456:Fluorescent Dyes