title: Serological detection of antibodies against Paracoccidioides brasiliensis in dogs with leishmaniasis.
abstract: The aim of this study was to detect antibodies against Paracoccidioides brasiliensis in dogs seropositive and seronegative for leishmaniasis. Sera from 836 dogs (449 positive and 387 negative to leishmaniasis) were analysed by ELISA and the immunodiffusion test using gp43 and exoantigen, respectively. The analysis of the 836 serum samples by ELISA and the immunodiffusion test showed a positivity of 67.8 % and 7.3%, respectively, for P. brasiliensis infection. The dogs positive to leishmaniasis showed a higher reactivity to gp43 (79.9%) and exoantigen (12.7%) than the negative ones (54.0% and 1.0%, respectively). The higher reactivity to P. brasiliensis antigens may be due to cross-reactivity or a co-infection of dogs by Leishmania and P. brasiliensis. The lower correlation (0.187) observed between reactivity to gp43 and Leishmania antigen reinforces the latter hypothesis.
authors.affiliation: Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Campus Universitário, Londrina, Paraná, 86051-980, Brazil.
authors.forename: L H
authors.initials: LH
authors.lastname: Silveira
authors.forename: I H
authors.initials: IH
authors.lastname: Domingos
authors.forename: K
authors.initials: K
authors.lastname: Kouchi
authors.forename: E N
authors.initials: EN
authors.lastname: Itano
authors.forename: E A
authors.initials: EA
authors.lastname: Silva
authors.forename: V O
authors.initials: VO
authors.lastname: Landgraf
authors.forename: S M
authors.initials: SM
authors.lastname: Werneck
authors.forename: Z P
authors.initials: ZP
authors.lastname: Camargo
authors.forename: M A
authors.initials: MA
authors.lastname: Ono
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D000946:Antigens, Fungal / Q000276:immunology; D001938:Brazil / Q000453:epidemiology; D015897:Comorbidity; D004283:Dog Diseases / Q000453:epidemiology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005656:Fungal Proteins / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D005779:Immunodiffusion; D007896:Leishmaniasis / Q000453:epidemiology*; D008297:Male; D010228:Paracoccidioides / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000453:epidemiology*; D016036:Seroepidemiologic Studies
chemical_list: C103180:43 kDa protein, Paracoccidioides; D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D005656:Fungal Proteins; D006023:Glycoproteins