Kaempferia parviflora ethanolic extract promoted nitric oxide production in human umbilical vein endothelial cells.
The rhizomes of Kaempferia parviflora (KP) (Zingiberaceae) have been used in Thai traditional medicine for health promotion and for the treatment of digestive disorders and gastric ulcer. This study investigated effect of KP on endothelial function. Studies in human umbilical vein endothelial cells (HUVEC) showed that KP dose-dependently increased nitrite concentrations in culture media after 48 h incubation. eNOS mRNA and protein expression were also enhanced. The induction of eNOS mRNA was detected at 4 h and plateau at 48 h while iNOS expression was not observed. These data demonstrate that KP has a great potential for a supplemental use in vascular endothelial health promotion.
Wattanapitayakul
Suvara K
SK
Department of Pharmacology, Srinakharinwirot University, Sukhumvit 23, Wattana, Bangkok 10110, Thailand. suvara@swu.ac.th
Suwatronnakorn
Maneewan
M
Chularojmontri
Linda
L
Herunsalee
Angkana
A
Niumsakul
Somchit
S
Charuchongkolwongse
Suphan
S
Chansuvanich
Nuchattra
N
D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008518:Medicine, East Asian Traditional; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000187:drug effects / Q000235:genetics; D009573:Nitrites / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000187:drug effects; D027343:Rhizome; D013785:Thailand; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism; D030521:Zingiberaceae / Q000737:chemistry*
D009573:Nitrites; D010936:Plant Extracts; D012333:RNA, Messenger; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III