title: Thrombin-activable fibrinolysis inhibitor (TAFI) zymogen is an active carboxypeptidase.
abstract: Thrombin-activable fibrinolysis inhibitor (TAFI) is a carboxypeptidase found in human plasma, presumably as an inactive zymogen. The current dogma is that proteolytic activation by thrombin/thrombomodulin generates the active enzyme (TAFIa), which down-regulates fibrinolysis by removing C-terminal lysine residues from partially degraded fibrin. In this study, we have shown that the zymogen exhibits continuous and stable carboxypeptidase activity against large peptide substrates, and we suggest that the activity down-regulates fibrinolysis in vivo.
authors.affiliation: Center for Insoluble Protein Structure at the Department of Molecular Biology, Science Park, University of Aarhus, Gustav Wieds Vej 10c, 8000 Aarhus C, Denmark.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Valnickova
authors.forename: Ida B
authors.initials: IB
authors.lastname: Thøgersen
authors.forename: Jan
authors.initials: J
authors.lastname: Potempa
authors.forename: Jan J
authors.initials: JJ
authors.lastname: Enghild
mesh_terms: D000595:Amino Acid Sequence; D025901:Carboxypeptidase B2 / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism; D005342:Fibrinolysis; D006706:Homeostasis; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D010958:Plasminogen / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity
chemical_list: D004792:Enzyme Precursors; D010446:Peptide Fragments; D010958:Plasminogen; D025901:Carboxypeptidase B2