Efficient purification of transglutaminase from recombinant Streptomyces platensis at various scales.
An efficient system for the fast and efficient purification of transglutaminase from recombinant Streptomyces platensis and expressed in Streptomyces lividans 25-2 is described. Because the purification procedure of this system is flexible, culture broth from laboratory (20 l) and pilot-plant (130 l) fermentations were used to purify the enzyme to electrophoretic homogeneity with high purity (90-95%) and yield (61-77%) within 1 or 2 days.
Lin
Shie-Jea
SJ
Food Industry Research and Development Institute, Hsinchu, Taiwan, ROC. lsj@firdi.org.tw
Hsieh
Yi-Fang
YF
Wang
Pei-Ming
PM
Chu
Wen-Shen
WS
D005591:Chemical Fractionation / Q000379:methods*; D024861:Genetic Enhancement / Q000379:methods*; D010865:Pilot Projects; D011995:Recombination, Genetic / Q000235:genetics; D013302:Streptomyces / Q000201:enzymology* / Q000378:metabolism*; D011503:Transglutaminases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D011503:Transglutaminases