TRB3 interacts with CtIP and is overexpressed in certain cancers.
TRB3, a human homolog of Drosophila Tribbles, has been recently shown as a critical negative regulator of Akt and S6 kinase activation in a number of cellular processes. Here we found that TRB3 interacted with an important cell cycle regulator CtIP (CtBP-interacting protein) and the interaction involved the C-terminus of both proteins. Interestingly, TRB3 and CtIP co-localized to the nucleus in HeLa cells and exhibited a unique dot-like pattern. Finally, we demonstrated that TRB3 was overexpressed in multiple tumor tissues. Since CtIP plays important roles in cell cycle checkpoint control and it has been implicated in tumorigenesis, our data suggest that TRB3 may be involved in these biological processes through interacting with CtIP.
Xu
Jianmin
J
Department of Surgery, Zhongshan Hospital, Fudan University Medical School, 180 Fenglin Rd., Shanghai 200032, China.
Lv
Shun
S
Qin
Yan
Y
Shu
Fang
F
Xu
Yanjuan
Y
Chen
Jian
J
Xu
Bing-e
BE
Sun
Xiaoqing
X
Wu
Jun
J
D001402:B-Lymphocytes; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D017931:DNA Primers; D004706:Endodeoxyribonucleases; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000201:enzymology / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism* / Q000037:antagonists & inhibitors; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D013601:T-Lymphocytes; D014162:Transfection
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D017931:DNA Primers; D009687:Nuclear Proteins; D010446:Peptide Fragments; D012097:Repressor Proteins; C474831:TRIB3 protein, human; D017346:Protein Serine-Threonine Kinases; D004706:Endodeoxyribonucleases; C410741:RBBP8 protein, human