title: Expression pattern of chemokine receptors and chemokine release in inflammatory erythroderma and Sézary syndrome.
abstract: BACKGROUND
  Erythroderma can be caused by inflammatory dermatoses or cutaneous T-cell lymphoma. Even if chemokines and their receptors are involved in the skin-selective lymphocyte recruitment, their role in inflammatory erythroderma is yet unclear.
  
  
  OBJECTIVE
  To evaluate the chemokine release (TARC, MDC, IP-10) and to define the expression pattern of Th1- (CCR5, CXCR3) and Th2-related (CCR4) chemokine receptors in inflammatory erythroderma and Sézary syndrome (SS).
  
  
  METHODS
  Flow cytometry has been carried out on both circulating and skin-infiltrating T lymphocytes; serum chemokine levels have been evaluated using ELISA techniques.
  
  
  RESULTS
  CCR4, CCR5 and CXCR3 were expressed on about 40% of peripheral blood lymphocytes and on the majority of skin-infiltrating lymphocytes in the inflammatory erythroderma patients, whereas the leukemic CD4+CD26- subpopulation in SS was characterized by a high CCR4 expression without a concurrent increase in CCR5 or CXCR3. TARC, MDC and IP-10 serum levels were significantly increased in both erythrodermic and SS patients.
  
  
  CONCLUSIONS
  Our results confirm that SS is a Th2 disorder with a selective expression of CCR4, whereas inflammatory erythroderma shares an overexpression of both Th1- and Th2-related chemokine receptors, suggesting an activation of different pathways driving reactive lymphocytes to the skin.
authors.affiliation: Section of Dermatology, Department of Medical Sciences and Human Oncology, University of Turin, Turin, Italy. mariateresa.fierro@unito.it
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Fierro
authors.forename: Alessandra
authors.initials: A
authors.lastname: Comessatti
authors.forename: Pietro
authors.initials: P
authors.lastname: Quaglino
authors.forename: Michela
authors.initials: M
authors.lastname: Ortoncelli
authors.forename: Simona
authors.initials: S
authors.lastname: Osella Abate
authors.forename: Renata
authors.initials: R
authors.lastname: Ponti
authors.forename: Mauro
authors.initials: M
authors.lastname: Novelli
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Bernengo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D054414:Chemokine CCL17; D018925:Chemokines / Q000097:blood / Q000378:metabolism*; D019742:Chemokines, CC / Q000032:analysis / Q000097:blood; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D003873:Dermatitis, Exfoliative / Q000097:blood / Q000276:immunology* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D054398:Receptors, CCR4; D019713:Receptors, CCR5 / Q000032:analysis / Q000097:blood; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000032:analysis* / Q000097:blood; D018121:Receptors, Cytokine / Q000032:analysis / Q000097:blood; D012751:Sezary Syndrome / Q000097:blood / Q000276:immunology* / Q000473:pathology; D012878:Skin Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: C106230:CCL17 protein, human; C515354:CCR4 protein, human; C515187:CXCR3 protein, human; D054414:Chemokine CCL17; D018925:Chemokines; D019742:Chemokines, CC; C104577:IP10-Mig receptor; D054398:Receptors, CCR4; D019713:Receptors, CCR5; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine