title: Use of sequence-based typing for investigation of a case of nosocomial legionellosis.
abstract: A fatal case of nosocomial legionellosis in a low prevalence region (Calgary, Alberta, Canada) prompted investigation into the source of infection. Hospital water systems contaminated with Legionella pneumophila have been shown to pose a risk to compromised patients. Typing of an L. pneumophila serogroup 1 strain isolated from the patient using sequence-based typing (SBT) and amplified fragment length polymorphism (AFLP) analysis linked it to a persistent and widespread strain isolated from the hospital water system establishing a nosocomial mode of acquisition. Different SBT and AFLP patterns were determined for non-epidemiologically linked cases and isolates from different hospitals.
authors.affiliation: Provincial Laboratory for Public Health (Microbiology), Calgary site, Calgary, Alberta, Canada.
authors.forename: Sallene
authors.initials: S
authors.lastname: Wong
authors.affiliation: Provincial Laboratory for Public Health (Microbiology), Calgary site, Calgary, Alberta, Canada.
authors.forename: Kanti
authors.initials: K
authors.lastname: Pabbaraju
authors.affiliation: Provincial Laboratory for Public Health (Microbiology), Calgary site, Calgary, Alberta, Canada.
authors.forename: Veronica F
authors.initials: VF
authors.lastname: Burk
authors.affiliation: Ontario Ministry of Health and Long-Term Care, Toronto, Ontario, Canada.
authors.forename: George C
authors.initials: GC
authors.lastname: Broukhanski
authors.affiliation: Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.|Provincial Laboratory for Public Health (Microbiology), Calgary site, Calgary, Alberta, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: Fox
authors.affiliation: Infection Prevention and Control, Calgary Health Region, Calgary, Alberta, Canada.
authors.forename: Tom
authors.initials: T
authors.lastname: Louie
authors.affiliation: Infection Prevention and Control, Calgary Health Region, Calgary, Alberta, Canada.
authors.forename: Manuel W
authors.initials: MW
authors.lastname: Mah
authors.affiliation: National Microbiology Laboratory, Winnipeg, Manitoba, Canada.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Bernard
authors.affiliation: Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.|Provincial Laboratory for Public Health (Microbiology), Calgary site, Calgary, Alberta, Canada.
authors.forename: Peter A G
authors.initials: PAG
authors.lastname: Tilley
mesh_terms: D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D002170:Canada / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000209:etiology*; D004269:DNA, Bacterial / Q000235:genetics; D017809:Fatal Outcome; D005260:Female; D006801:Humans; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007876:Legionellosis / Q000453:epidemiology / Q000209:etiology*; D008666:Metalloendopeptidases / Q000235:genetics; D008969:Molecular Sequence Data; D018410:Pneumonia, Bacterial / Q000453:epidemiology / Q000209:etiology*; D012150:Polymorphism, Restriction Fragment Length; D018272:Porins / Q000235:genetics; D012307:Risk Factors; D020539:Sequence Analysis, Protein; D013045:Species Specificity; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D018272:Porins; C072453:major outer membrane protein, Legionella pneumophila; D008666:Metalloendopeptidases