The 53-kDa proteolytic product of precursor starch-hydrolyzing enzyme of Aspergillus niger has Taka-amylase-like activity.
The 53-kDa amylase secreted by Aspergillus niger due to proteolytic processing of the precursor starch-hydrolyzing enzyme was resistant to acarbose, a potent alpha-glucosidase inhibitor. The enzyme production was induced when A. niger was grown in starch medium containing the inhibitor. Antibodies against the precursor enzyme cross-reacted with the 54-kDa Taka-amylase protein of A. oryzae. It resembled Taka-amylase in most of its properties and also hydrolyzed starch to maltose of alpha-anomeric configuration. However, it did not degrade maltotriose formed during the reaction and was not inhibited by zinc ions.
Ravi-Kumar
K
K
Department of Food Microbiology, Central Food Technological Research Institute, Mysore 570020, India. r-kadeppagari@northwestern.edu
Venkatesh
K S
KS
Umesh-Kumar
S
S
D001234:Aspergillus niger / Q000201:enzymology*; D006868:Hydrolysis; D010447:Peptide Hydrolases / Q000502:physiology*; D011498:Protein Precursors / Q000502:physiology*; D013213:Starch / Q000378:metabolism*; D000516:alpha-Amylases / Q000502:physiology*
D011498:Protein Precursors; D013213:Starch; D000516:alpha-Amylases; D010447:Peptide Hydrolases