Analysis of expressed sequence tags from skeletal muscle-specific cDNA library of Chinese native Xiang pig.
A Longissimus Dorsi muscle cDNA library of Xiang Pig was constructed, and 131 randomly isolated clones were sequenced in this study. The results of bioinformatics analysis showed that 131 ESTs represented 109 unique clones sequences, of which 99 showed homology to previously identified genes in humans or other mammals, 3 matched other uncharacterized expressed sequence tags (ESTs), and 7 showed no significant matches to sequences already present in DNA databases. No protein matches were found for 10 ESTs. Functional analysis of the ESTs showed that a considerable proportion of them encoded proteins involved in gene/protein expression (45.46%). Other classes included genes involved in metabolism (10.10%), cell structure/motility (10.10%), cell/organism defense (5.05%), cell signaling/communication (2.02%), and cell division (0.0%). Unclassified genes constituted the remaining 27.27%. This study reported the results of the first gene expression profile analysis of Chinese native Xiang Pig skeletal muscle cells, thereby greatly facilitating the functional study of candidate genes involved in muscle growth as well as in the improvement of meat quality in domestic pigs.
Wang
Xiu-Li
XL
The National Laboratories for Agrobiotechnobogy, China Agricultural University, Beijing 100094, China.
Wu
Ke-Liang
KL
Li
Ning
N
Li
Chang-Lv
CL
Qiu
Xue-Mei
XM
Wang
Ai-Hua
AH
Wu
Chang-Xin
CX
D000818:Animals; D002681:China; D003001:Cloning, Molecular; D019295:Computational Biology; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015723:Gene Library; D018482:Muscle, Skeletal / Q000378:metabolism*; D016366:Open Reading Frames; D009928:Organ Specificity; D017422:Sequence Analysis, DNA; D013552:Swine / Q000145:classification* / Q000235:genetics*