[Determination in vitro of rat plasma protein binding rate of sinomenine by using microdialysis method].
OBJECTIVE
To determine in vitro the rat plasma protein binding rate by using microdialysis method.


METHODS
The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method. The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate. The x-intercept of regression equation was the free drug concentration (Cf). The plasma protein binding rate was calculated by using the following equation: f = ( C0 - Cf)/C0.


RESULTS
The binding rate was kept relatively stable in the studied concentration range.


CONCLUSIONS
It is feasible that the plasma protein binding rate can be determined by using microdialysis method.
Zhang
Ying-feng
YF
Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China. fredzhangfeng@163.com
Zhou
Li-ling
LL
Li
Rui
R
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D017551:Microdialysis / Q000379:methods*; D009019:Morphinans / Q000302:isolation & purification / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis; D032841:Sinomenium / Q000737:chemistry
D001798:Blood Proteins; D009019:Morphinans; C009271:sinomenine