title: [Separation and identification of Taxol in the crude extracts of Taxus cuspidata and its callus culture with HPLC-ESI-MS/MS].
abstract: OBJECTIVE
  To study the MS/MS fragmentation mechanism of Taxol, and based on it to establish HPLC-ESI-MS/MS technique to separate and identify Taxol in the crude extracts of Taxus cuspidata and its callus culture, consequently to provide a fast and credible method for the analysis of Taxol in natural products.
  
  
  METHODS
  Optimized the HPLC-ESI-MS/MS parameters for the sample analysis, and then discussed the ionization and cleavage mechanism of Taxol in ESI-MS and ESI-MS/MS, finally identified the Taxol in the samples with retention time, molecular weight and MS/MS spectra.
  
  
  RESULTS
  Elucidated the MS/MS fragmentation mechanism of Taxol, and developed HPLC-ESI-MS/MS method to analyze Taxol in the two samples.
  
  
  CONCLUSIONS
  The HPLC-ESI-MS/MS method is rapid, highly sensitive and specific, so it is suitable for the separation and identification of Taxol in natural products.
authors.affiliation: National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Ji-cheng
authors.initials: JC
authors.lastname: Duan
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liang
authors.forename: Wei-bing
authors.initials: WB
authors.lastname: Zhang
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Zhang
authors.forename: Yu-shu
authors.initials: YS
authors.lastname: Huo
authors.forename: Yu-kui
authors.initials: YK
authors.lastname: Zhang
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D017239:Paclitaxel / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry; D020946:Taxus / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D017239:Paclitaxel