Calcitonin gene-related peptide inhibits early B cell development in vivo.
Recent in vitro studies suggest that calcitonin gene-related peptide (CGRP) inhibits early B cell differentiation; however, there is no evidence in the intact animal for a role for CGRP in B cell development. Here, we show that in vivo treatment of mice with CGRP reduces the number of IL-7 responsive B cell progenitors in bone marrow. A single CGRP treatment reduces IL-7-responsive B cell progenitors by up to 40% for up to 72 h. The reduction is dose-dependent and can be blocked by a CGRP receptor antagonist, CGRP(8-37). CGRP in serum following injection is highly elevated at 30 min but returns to basal levels by 4 h, suggesting that a single injection of CGRP has long-lasting effects on B cell development. This report provides the first direct in vivo evidence that CGRP, a neuropeptide with multiple effects on mature lymphocytes, also plays a regulatory role in early B cell development in the bone marrow.
Schlomer
Jerome J
JJ
Department of Microbiology, University of Kentucky College of Medicine, Lexington, KY 40536, USA.
Storey
Benjamin B
BB
Ciornei
Radu-Tudor
RT
McGillis
Joseph P
JP
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology; D015740:Calcitonin Gene-Related Peptide / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D003114:Colony-Forming Units Assay; D007275:Injections, Intravenous; D015851:Interleukin-7 / Q000037:antagonists & inhibitors / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013329:Structure-Activity Relationship
D015851:Interleukin-7; D015740:Calcitonin Gene-Related Peptide