title: Use of partial 16S rRNA gene sequencing for identification of Legionella pneumophila and non-pneumophila Legionella spp.
abstract: We examined 49 Legionella species, 26 L. pneumophila and 23 non-pneumophila Legionella spp., using partial 16S rRNA gene sequencing. This approach accurately identified all the L. pneumophila isolates, characterized all non-pneumophila Legionella isolates as such within this genus, and classified most (20/23; 87%) of the non-pneumophila Legionella isolates to the species level.
authors.affiliation: The Cleveland Clinic, Ohio, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Wilson
authors.forename: U
authors.initials: U
authors.lastname: Reischl
authors.forename: G S
authors.initials: GS
authors.lastname: Hall
authors.forename: G W
authors.initials: GW
authors.lastname: Procop
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial; D020459:Genes, rRNA; D006801:Humans; D007875:Legionella / Q000145:classification* / Q000235:genetics; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S