Lipopolysaccharide-binding and neutralizing activities of surfactin C in experimental models of septic shock.
To evaluate the anti-endotoxin activity of surfactin C, we studied its lipopolysaccharide-binding activity in vitro and therapeutic efficacy in experimental models of gram-negative septic shock. The ability of surfactin C to bind LPS from Escherichia coli O111:B4 was determined using a limulus chromogenic assay. Male ICR mice and Sprague-Dawley rats were given intraperitoneal administration of 1x10(9) colony forming units of E. coli ATCC 25922. After bacterial challenge, all animals were randomized to receive intraperitoneally saline, polymyxin B or surfactin C. Surfactin C not only completely bound to the LPS (its median effective concentration being 13.75 microM) but also improved the survival and reduced of the number of inoculated bacteria in the mouse model of septic shock. Surfactin C reduced the plasma endotoxin, tumor necrosis factor-alpha and nitric oxide levels in response to septic shock in rats.
Hwang
Youn-Hwan
YH
College of Veterinary Medicine, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon, Korea.
Park
Byung-Kwon
BK
Lim
Jong-Hwan
JH
Kim
Myoung-Seok
MS
Park
Seung-Chun
SC
Hwang
Mi-Hyun
MH
Yun
Hyo-In
HI
D000818:Animals; D004731:Endotoxins / Q000097:blood; D004926:Escherichia coli; D055666:Lipopeptides; D008070:Lipopolysaccharides / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000097:blood; D010456:Peptides, Cyclic / Q000378:metabolism / Q000627:therapeutic use*; D011112:Polymyxin B / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D012772:Shock, Septic / Q000382:microbiology / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D004731:Endotoxins; D055666:Lipopeptides; D008070:Lipopolysaccharides; D010456:Peptides, Cyclic; D014409:Tumor Necrosis Factor-alpha; C009365:surfactin peptide; D009569:Nitric Oxide; D011112:Polymyxin B