title: Early diagnosis of rickettsioses by electrochemiluminscence.
abstract: The diagnosis of acute rickettsioses during the acute phase of the disease is challenging. We present preliminary evidence that antigen-capture using streptavidin-coated magnetic beads with biotinylated anti-rickettsia typhi rabbit polyclonal antibodies followed by electrochemiluminescent detection with ruthenylated antibodies of the same specificity could be used for the diagnosis of rickettsial diseases in the acute phase.
authors.affiliation: Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, R. 0609, Galveston, TX 77555-0609, USA.
authors.forename: Gary
authors.initials: G
authors.lastname: Wen
authors.forename: Jere W
authors.initials: JW
authors.lastname: McBride
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhang
authors.forename: Juan P
authors.initials: JP
authors.lastname: Olano
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000302:isolation & purification; D004563:Electrochemistry / Q000379:methods; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D011817:Rabbits; D012282:Rickettsia Infections / Q000175:diagnosis*; D012286:Rickettsia typhi; D014437:Typhus, Endemic Flea-Borne / Q000175:diagnosis
chemical_list: D000907:Antibodies, Bacterial