Evaluation of an alkaline phosphatase-labeled oligonucleotide probe for the detection and enumeration of the thermostable-related hemolysin (trh) gene of Vibrio parahaemolyticus.
Reliable methods are needed to detect total and pathogenic Vibrio parahaemolyticus. One marker of V. parahaemolyticus virulence is the thermostable-related hemolysin. We developed an alkaline phosphatase-labeled DNA probe method for the specific detection and enumeration of trh-positive V. parahaemolyticus by colony hybridization. The probe was tested against a panel of 200 bacterial strains and determined to be specific for trh-positive V. parahaemolyticus. Additionally, the trh alkaline phosphatase probe colony hybridization was successfully used to detect and enumerate trh-positive V. parahaemolyticus in seafood and water samples collected from the United States and the United Kingdom.
Nordstrom
Jessica L
JL
U.S. Food and Drug Administration, Gulf Coast Seafood Laboratory, 1 Iberville Drive, P.O. Box 158, Dauphin Island, Alabama 36528, USA. jessica.nordstrom@fda.hhs.gov
Rangdale
Rachel
R
Vickery
Michael C L
MC
Phillips
Andrea M B
AM
Murray
Shelley L
SL
Wagley
Sariqa
S
DePaola
Angelo
A
D000469:Alkaline Phosphatase; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification*; D003257:Consumer Product Safety; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006460:Hemolysin Proteins / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D015345:Oligonucleotide Probes; D017747:Seafood / Q000382:microbiology*; D013045:Species Specificity; D006113:United Kingdom; D014481:United States; D014736:Vibrio parahaemolyticus / Q000235:genetics*; D014871:Water Microbiology
D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D015345:Oligonucleotide Probes; C073845:hemolysin, Vibrio; D000469:Alkaline Phosphatase