title: Antiplasmodial and GABA(A)-benzodiazepine receptor binding activities of five plants used in traditional medicine in Mali, West Africa.
abstract: Extracts of five medicinal plants: Boscia angustifolia, Cissus quadrangularis, Securidaca longipedunculata, Stylosanthes erecta and Trichilia emetica, used traditionally in Malian traditional medicine were screened for in vitro antiplasmodial activity and GABA(A)-benzodiazepine receptor binding activity. Four extracts showed significant antiplasmodial activities, with the dichloromethane extract of leaf of Securidaca longipedunculata being the most active (IC(50) of 7 microg/ml [95% CI: 5-9]). The dichloromethane extract of leaf of Trichilia emetica, in addition to its antiplasmodial activity (IC(50): 12 microg/ml [95% CI: 12-14]), exhibited a good binding activity to the GABA(A)-benzodiazepine receptor, while water and methanol extracts of the same plant did not show any activity. A strong GABA(A)-receptor complex binding activity was observed in the methanol extract of aerial part of Stylosanthes erecta. The results in this study justify some of the traditional indications of the plants investigated and may thus be candidates for Improved Traditional Medicines in Mali.
authors.affiliation: University of Oslo, School of Pharmacy, Department of Pharmaceutical Chemistry, PO Box 1068 Blindern, N-0316 Oslo, Norway.
authors.forename: Sekou
authors.initials: S
authors.lastname: Bah
authors.forename: Anna K
authors.initials: AK
authors.lastname: Jäger
authors.forename: Anne
authors.initials: A
authors.lastname: Adsersen
authors.forename: Drissa
authors.initials: D
authors.lastname: Diallo
authors.forename: Berit Smestad
authors.initials: BS
authors.lastname: Paulsen
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects / Q000469:parasitology; D008288:Malaria / Q000188:drug therapy; D008302:Mali; D016488:Medicine, African Traditional; D000432:Methanol; D008752:Methylene Chloride; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000187:drug effects*; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism; D014867:Water
chemical_list: D000962:Antimalarials; D010936:Plant Extracts; D011963:Receptors, GABA-A; D014867:Water; D008752:Methylene Chloride; D000432:Methanol