title: Human eosinophil major basic protein 2: location of disulfide bonds and free sulfhydryl groups.
abstract: Eosinophil granule major basic protein 2 (MBP2 or major basic protein homolog) is a paralog of major basic protein (MBP1) and, similar to MBP1, is cytotoxic and cytostimulatory in vitro. MBP2, a small protein of 13,433 Da molecular weight, contains 10 cysteine residues. Mass spectrometry shows two cystine disulfide linkages (Cys20-Cys115 and Cys92-Cys107) and 6 cysteine residues with free sulfhydryl groups (Cys2, Cys23, Cys42, Cys43, Cys68, and Cys96). MBP2, similar to MBP1, has conserved motifs in common with C-type lectins. The disulfide bond locations are conserved among human MBP1, MBP2 and C-type lectins.
authors.affiliation: Department of Dermatology, School of Medicine, University of Utah, Room 4RI22 SOM 30 North 1900 East, Salt Lake City, Utah, 84132, USA. lori.wagner@hsc.utah.edu
authors.forename: Lori A
authors.initials: LA
authors.lastname: Wagner
authors.forename: Lyo E
authors.initials: LE
authors.lastname: Ohnuki
authors.forename: Krishna
authors.initials: K
authors.lastname: Parsawar
authors.forename: Gerald J
authors.initials: GJ
authors.lastname: Gleich
authors.forename: Chad C
authors.initials: CC
authors.lastname: Nelson
mesh_terms: D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry; D003553:Cystine / Q000032:analysis* / Q000737:chemistry*; D047093:Eosinophil Major Basic Protein; D005033:Ethylmaleimide / Q000737:chemistry; D006801:Humans; D010449:Peptide Mapping; D011509:Proteoglycans / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D016415:Sequence Alignment; D021241:Spectrometry, Mass, Electrospray Ionization; D014357:Trypsin / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D011509:Proteoglycans; C074129:PRG2 protein, human; D003553:Cystine; D047093:Eosinophil Major Basic Protein; D014357:Trypsin; D003545:Cysteine; D005033:Ethylmaleimide