Age-dependent increase of discoidin domain receptor 2 and matrix metalloproteinase 13 expression in temporomandibular joint cartilage of type IX and type XI collagen-deficient mice.
Our previous studies demonstrated that mutations in type IX and type XI collagens in mice caused osteoarthritis (OA)-like changes in knee and temporomandibular (TM) joints. We also found that the overexpression of matrix metalloproteinase 13 (Mmp-13) was probably due to the up-regulation of a collagen receptor, discoidin domain receptor 2 (Ddr2), which was responsible for knee cartilage degeneration in mutant mice. The objective of our study was to determine whether the expression of Mmp-3, Mmp-13 and Ddr2 was increased in OA-like TM joints in mutant mice using immunohistochemistry. We found that the staining for Ddr2, Mmp-13 and Mmp-derived type II collagen fragments in tissue sections from 6-month-old mice was increased in TM joints of the mutant mice. In contrast, we found no difference in the staining for Mmp-3 amongst the two mutant mice and their wild-type littermates. We conclude that, similar to previous observations in knee joints, the overexpression of Ddr2 and Mmp-13 may be responsible for the OA-like change in TM joints in mutant mice.
Lam
N P
NP
Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA 02115, USA.
Li
Y
Y
Waldman
A B
AB
Brussiau
J
J
Lee
P L
PL
Olsen
B R
BR
Xu
L
L
D000375:Aging / Q000473:pathology*; D000818:Animals; D002358:Cartilage, Articular / Q000201:enzymology / Q000473:pathology*; D024043:Collagen Type II / Q000032:analysis; D024081:Collagen Type IX / Q000172:deficiency* / Q000235:genetics; D024161:Collagen Type XI / Q000172:deficiency* / Q000235:genetics; D000070818:Discoidin Domain Receptors; D004195:Disease Models, Animal; D005838:Genotype; D006579:Heterozygote; D007150:Immunohistochemistry; D053509:Matrix Metalloproteinase 13 / Q000032:analysis*; D019278:Matrix Metalloproteinase 3 / Q000032:analysis; D051379:Mice; D008817:Mice, Mutant Strains; D009154:Mutation / Q000235:genetics; D010003:Osteoarthritis / Q000201:enzymology / Q000473:pathology*; D010009:Osteochondrodysplasias / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis*; D011975:Receptors, Mitogen / Q000032:analysis*; D013705:Temporomandibular Joint Disorders / Q000201:enzymology / Q000473:pathology*
D024043:Collagen Type II; D024081:Collagen Type IX; D024161:Collagen Type XI; D011975:Receptors, Mitogen; D000070818:Discoidin Domain Receptors; D020794:Receptor Protein-Tyrosine Kinases; D053509:Matrix Metalloproteinase 13; D019278:Matrix Metalloproteinase 3