Evaluation of SAT-1, SAT-2 and GalNAcT-1 mRNA in colon cancer by real-time PCR.
By qualitative and quantitative PCR, we evaluated the expression of three messengers coding for SAT-1, SAT-2 and GalNAcT-1 in human samples of intestinal cancer and some cell lines (breast cancer and melanomas). Qualitative PCR demonstrated, in human tissues but not in the cell lines examined, the presence of an mRNA that lacks hexon 3; experiments performed on transfected SKMEL-28 excluded a regulative role of this noncanonical mRNA. Data from real-time PCR, statistically analysed by ANOVA indicated that the mRNA expression of all the considered glycosyltransferases (SAT-1, SAT-2 and GalNAcT-1) was significantly different in tumours versus their own control. The ganglioside patterns in the examined samples did not correlate with mRNA expression; this finding demonstrates that ganglioside expression is the result of a very complex balance between anabolic and catabolic enzyme activities. Although this study is still preliminary, it opens a new possibility for neoplastic prognosis finding potential molecular markers among the mRNAs that codify for glycosyltransferases.
Gornati
Rosalba
R
Dipartimento di Biotecnologie e Scienze Molecolari, Università dell'Insubria, Via J.H. Dunant 3, 21100, Varese, Italy. rosalba.gornati@uninsubria.it
Chini
Valentina
V
Rimoldi
Simona
S
Meregalli
Maurizio
M
Schiaffino
Eugenio
E
Bernardini
Giovanni
G
D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D000368:Aged; D000704:Analysis of Variance; D027321:Anion Transport Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D045744:Cell Line, Tumor; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D003110:Colonic Neoplasms / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D005260:Female; D005732:Gangliosides / Q000032:analysis / Q000096:biosynthesis; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D000076988:Sulfate Transporters; D014162:Transfection; D000097763:Polypeptide N-acetylgalactosaminyltransferase; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D027321:Anion Transport Proteins; D005732:Gangliosides; D017350:N-Acetylgalactosaminyltransferases; D012333:RNA, Messenger; D000076988:Sulfate Transporters; D000097763:Polypeptide N-acetylgalactosaminyltransferase; D000099228:Diamine N-Acetyltransferase; C473560:SLC26A1 protein, human