Isolation and identification of alpha-amylase producing Bacillus sp. from dhal industry waste.
A bacterial strain was isolated from dhal industry red gram waste and identified as Bacillus. A thermostable extracellular amylase was partially purified from the strain. Optimum temperature and pH for the enzyme were found to be 60 degrees C and 6.5, respectively. The maximum amylase production was achieved with maltose as carbon source. Among the nitrogen sources, peptone and yeast extract produced maximum amylase.
Thippeswamy
S
S
Department of Studies in Biochemistry, Gulbarga University, Gulbarga 585 106, Karnataka, India.
Girigowda
K
K
Mulimani
V H
VH
D000681:Amylases / Q000737:chemistry; D001407:Bacillus / Q000201:enzymology* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000737:chemistry; D019649:Food Industry / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D007221:Industry; D009844:Oligosaccharides / Q000737:chemistry; D013213:Starch; D013696:Temperature; D000516:alpha-Amylases / Q000378:metabolism / Q000502:physiology*
D007220:Industrial Waste; D009844:Oligosaccharides; D002244:Carbon; D013213:Starch; D000681:Amylases; D000516:alpha-Amylases