title: Ocular lesions and experimental choline deficiency.
abstract: Previous studies have shown ocular haemorrhages in choline-deficient rats. The aim of this paper is to study further the relationship between ocular and renal lesions and biochemical alterations in rats fed a choline-deficient diet. Fifty one weanling male Wistar rats, were divided into two groups. Thirty one of them were fed a choline-deficient diet and the rest was fed a choline-supplemented diet ad libitum. Animals from both groups were killed between the fifth and the eighth day. Urea, creatinine and homocysteine concentrations in blood were determined. Eyes were used for light microscopy study; high resolution light microscopy and the study of the retina as "rétine a plat". Kidneys were studied by light microscopy. Choline-supplemented rats did not show ocular or renal lesion. Choline-deficient rats that showed renal lesions, tubular or cortical necrosis, did not always have ocular changes. There were no ocular changes in the only choline-deficient rat without renal lesion. The ocular changes consisted mainly in haemorrhage in both cameras and ciliary and vitreous bodies. Correlations between ocular and renal lesion (r = 0.72, p < 0.0001, CI 95%: 0.48-0.86); ocular lesion and creatinine (r = 0.86, p < 0.0001, Cl 95%: 0.72-0.93) and ocular lesion and urea (r = 0.70, p < 0.0001, Cl 95%: 0.44-0.85) were positive. Choline-deficiency induces ocular haemorrhagic lesions after the development of renal necrosis. The ocular pathology could be due to the immaturity of the ocular vasculature at this age. The hyaloid, choroid and retinal system are involved.
authors.affiliation: Laboratorio de Investigaciones Oftalmológicas y Visuales, Centro de Patología Experimental, Departamento de Patología, Facultad de Medicina, Universidad de Buenos Aires, Argentina. georginaossani@yahoo.com.ar
authors.forename: Georgina P
authors.initials: GP
authors.lastname: Ossani
authors.forename: David
authors.initials: D
authors.lastname: Pelayes
authors.forename: María L
authors.initials: ML
authors.lastname: Diaz
authors.forename: Nestor R
authors.initials: NR
authors.lastname: Lago
authors.forename: Silvia L
authors.initials: SL
authors.lastname: Fariña
authors.forename: Alberto J
authors.initials: AJ
authors.lastname: Monserrat
authors.forename: Jorge O
authors.initials: JO
authors.lastname: Zarate
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002796:Choline Deficiency / Q000150:complications / Q000473:pathology*; D003404:Creatinine / Q000097:blood; D004032:Diet; D004195:Disease Models, Animal; D005123:Eye / Q000098:blood supply / Q000648:ultrastructure*; D005131:Eye Injuries / Q000150:complications / Q000473:pathology*; D006710:Homocysteine / Q000097:blood; D007673:Kidney Cortex Necrosis / Q000209:etiology / Q000473:pathology*; D007683:Kidney Tubular Necrosis, Acute / Q000209:etiology / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012166:Retinal Hemorrhage / Q000209:etiology / Q000473:pathology; D012720:Severity of Illness Index; D014508:Urea / Q000097:blood
chemical_list: D006710:Homocysteine; D014508:Urea; D003404:Creatinine