Gene expression profiles of metabolic enzyme transcripts in Alzheimer's disease.
The successfully functioning brain is a heavy user of metabolic energy. Alzheimer's disease, in which cognitive faculties decline, may be due, at least in part, to metabolic insufficiency. Using microarray analysis and quantitative RT-PCR, the expression of mRNA transcripts involved in glucose metabolism was investigated in Alzheimer's diseased post-mortem human hippocampal samples. Of the 51 members of the glycolytic, tricarboxylic acid cycle, oxidative phosphorylation, and associated pathways investigated by qPCR, 15 were confirmed to be statistically significantly (p<0.05) down-regulated in Alzheimer's disease. This finding suggests that reductions in the levels of transcripts encoded by genes that participate in energy metabolism may be involved in Alzheimer's disease.
Brooks
Wendy M
WM
Medical Research Council Rosalind Franklin Centre for Genomics Research, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SB, UK.
Lynch
Patrick J
PJ
Ingle
Catherine C
CC
Hatton
Alexander
A
Emson
Piers C
PC
Faull
Richard L M
RL
Starkey
Mike P
MP
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000201:enzymology* / Q000235:genetics* / Q000503:physiopathology; D001921:Brain / Q000201:enzymology* / Q000503:physiopathology; D002952:Citric Acid Cycle / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D004798:Enzymes / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D006019:Glycolysis / Q000235:genetics; D006801:Humans; D008297:Male; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010085:Oxidative Phosphorylation; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004798:Enzymes; D012333:RNA, Messenger