Non-lethal heat shock protects gnotobiotic Artemia franciscana larvae against virulent Vibrios.
Brine shrimp Artemia were exposed under gnotobiotic conditions to a non-lethal heat shock (NLHS) from 28 to 32, 37 and 40 degrees C. Different recovery periods (2, 6, 12 and 24h) and different heat-exposure times (15, 30, 45 and 60 min) were tested. After these NLHS, Artemia was subsequently challenged with Vibrio. Challenge tests were performed in stressed and unstressed nauplii at concentrations of 10(7) cells ml(-1) of pathogenic bacteria, Vibrio campbellii and Vibrio proteolyticus. A NLHS with an optimal treatment of 37 degrees C for 30 min and a subsequent 6h recovery period resulted in a cross-protection against pathogenic Vibrio. A 100% increase in the larval survival (P < 0.05) was observed. We have also demonstrated by Western blot that a NLHS increases the expression of HSP-70 in heat-shocked (HS) treated animals. This report is the first to reveal a cross protection of a NLHS against deleterious bacterial challenges in living crustaceans. The putative role of heat shock proteins (HSPs) in this process is discussed.
Yik Sung
Yeong
Y
Laboratory for Aquaculture & Artemia Reference Center, Faculty of Bioscience Engineering, Ghent University, Rozier 44, 9000 Gent, Belgium. yeong@kustem.edu.my
Van Damme
Els J M
EJ
Sorgeloos
Patrick
P
Bossier
Peter
P
D000818:Animals; D001156:Artemia / Q000382:microbiology*; D015153:Blotting, Western; D005856:Germ-Free Life; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*; D006358:Hot Temperature; D007814:Larva / Q000382:microbiology; D016019:Survival Analysis; D013997:Time Factors; D014733:Vibrio / Q000472:pathogenicity*; D014774:Virulence
D018840:HSP70 Heat-Shock Proteins