Structural and mechanistic insights into hepatitis C viral translation initiation.
Hepatitis C virus uses an internal ribosome entry site (IRES) to control viral protein synthesis by directly recruiting ribosomes to the translation-start site in the viral mRNA. Structural insights coupled with biochemical studies have revealed that the IRES substitutes for the activities of translation-initiation factors by binding and inducing conformational changes in the 40S ribosomal subunit. Direct interactions of the IRES with initiation factor eIF3 are also crucial for efficient translation initiation, providing clues to the role of eIF3 in protein synthesis.
Fraser
Christopher S
CS
Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA.
Doudna
Jennifer A
JA
D020121:5' Untranslated Regions; D001665:Binding Sites; D039621:Eukaryotic Initiation Factor-3 / Q000737:chemistry / Q000378:metabolism; D016174:Hepacivirus / Q000235:genetics* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D020836:Protein Structure, Quaternary; D012315:RNA Caps / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*
D020121:5' Untranslated Regions; D039621:Eukaryotic Initiation Factor-3; D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins