Glia maturation factor gamma (GMFG): a cytokine-responsive protein during hematopoietic lineage development and its functional genomics analysis.
Human hematopoiesis was evaluated using the techniques of controlled stem cell differentiation, two-dimensional gel electrophoresis-based proteomics, and functional genomics. We provide the first report that glia maturation factor gamma (GMFG) is a cytokine-responsive protein in erythropoietin-induced and granulocyte-colony stimulating factor-induced hematopoietic lineage development. Results from global functional genomics analysis indicate that GMFG possesses several other features: hematopoietic tissue-specific gene expression, a promoter concentrated with high-score hematopoiesis-specific transcription factors, and possible molecular coevolution with a rudimentary blood/immune system. On the basis of our findings, we hypothesize that GMFG is a hematopoietic-specific protein that may mediate the pluripotentiality and lineage commitment of human hematopoietic stem cells.
Shi
Ying
Y
Molecular and Clinical Hematology Branch, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, MD 20892-1822, USA. yingshi@mail.nih.gov
Chen
Ling
L
Liotta
Lance A
LA
Wan
Hong-Hui
HH
Rodgers
Griffin P
GP
D000595:Amino Acid Sequence; D001854:Bone Marrow Cells / Q000166:cytology; D019070:Cell Lineage; D019295:Computational Biology / Q000379:methods; D016207:Cytokines / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004921:Erythropoietin / Q000378:metabolism; D023281:Genomics / Q000379:methods*; D020904:Glia Maturation Factor / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D040901:Proteomics / Q000379:methods; D017386:Sequence Homology, Amino Acid
D016207:Cytokines; D020904:Glia Maturation Factor; C117487:glia maturation factor gamma; D004921:Erythropoietin