Identification of MCP/CD46 analogue on bovine erythrocytes using the new monoclonal antibody IVA-520.
MCP/CD46 is a widely distributed C3b/C4b binding regulatory glycoprotein of the complement system that has been identified on all human peripheral blood cells except erythrocytes. In this paper, we describe the identification of bovine CD46 on all blood cells, including erythrocytes, with the newly prepared monoclonal antibody IVA-520. This antibody cross-reacts with human and pig cells. Furthermore, the molecule identified by IVA-520 functionally behaves as the MCP molecule, showing cofactor activity for the factor I-mediated cleavage of bovine C3 complement factor.
Antalíková
Jana
J
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Moyzesova 61, 900 28 Ivanka pri Dunaji, Slovak Republic. Jana.Antalikova@savba.sk
Simon
Michal
M
Jankovicová
Jana
J
Horovská
Lubica
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D051916:Membrane Cofactor Protein / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D013552:Swine
D000911:Antibodies, Monoclonal; D051916:Membrane Cofactor Protein