title: Experimental characterization and modelling of analytical monolithic column.
abstract: Hydrodynamics, equilibrium and kinetics of adsorption in a silica-based monolithic column Chromolith Performance RP-18e (Merck KgaA, Germany) have been studied. The column permeability was calculated according to the Darcy law for laminar flow. The efficiency of the monolithic column was characterized through the height equivalent to a theoretical plate (HETP) for myoglobin, phenol and progesterone. The 2-D single channel mathematical model has been applied to describe the adsorption dynamics. Parabolic velocity profile, axial and radial diffusion in the monolith channel, linear driving force model for the mass transfer in the monolith channel skeleton wall and linear adsorption equilibrium were assumed. The mathematical model gives good prediction of the experimental elution peaks.
authors.affiliation: Laboratory of Separation and Reaction Engineering (LSRE), Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal.
authors.forename: Michal
authors.initials: M
authors.lastname: Zabka
authors.forename: Mirjana
authors.initials: M
authors.lastname: Minceva
authors.forename: Alírio E
authors.initials: AE
authors.lastname: Rodrigues
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D008855:Microscopy, Electron, Scanning; D008962:Models, Theoretical; D009211:Myoglobin / Q000302:isolation & purification; D019800:Phenol / Q000302:isolation & purification; D011374:Progesterone / Q000302:isolation & purification; D012822:Silicon Dioxide
chemical_list: D009211:Myoglobin; D019800:Phenol; D011374:Progesterone; D012822:Silicon Dioxide