[Tear antioxidant potential in young adults].
OBJECTIVE
To study the antioxidant status of human tears, stimulated by onion fumes and the possible influence of the life habits thereon were measured.


METHODS
Subjects were ten healthy young adults, who answered a questionnaire about smoking, alcohol ingestion, fruit, vegetable, cereal, and vitamin intake and/or intake of other drugs, and physical exercise habits. Chemoluminescensce of luminol was used to analyze the total reactive antioxidant potential (TRAP), inhibition of piragollol was used to measure superoxide dismutase (SOD) and absorbance of H2O2 at 240 nm was used to identify catalase.


RESULTS
Mean +/- SD value for total reactive antioxidant potential was 33.8+/-11.5 microM and for superoxide dismutase 10.8+/-1.9 U/mL. Catalase was not identified. Regular exercise was associated with increased total reactive antioxidant potential values (p=0.021), with a difference of 18.6 microM between individuals who exercise at least once a week and sedentary individuals. Male gender and total reactive antioxidant potential values were statistically associated (p=0.013), with a difference of 16.3 microM between genders. There was an association between smoking and increased superoxide dismutase values (p=0.041), with a difference of 3.3 U/mL between smokers of more than five cigarettes/day and non-smokers. Also, vitamin C intake and superoxide dismutase values were associated (p=0.018); the difference for vitamin C takers was 3.3 U/mL.


CONCLUSIONS
Reflex tearing antioxidants were measurable in healthy young adults, and different variables apparently influenced their values.
Gus
Patrícia Ioschpe
PI
Universidade de São Paulo, São Paulo, SP, Brasil.
Belló-Klein
Adriane
A
Llesuy
Susana
S
Quinto
Guilherme Goulart
GG
Matos
Guilherme Herrmann
GH
Bechara
Samir Jacob
SJ
D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D002374:Catalase / Q000378:metabolism*; D015444:Exercise; D005260:Female; D006801:Humans; D008019:Life Style; D049449:Luminescence; D008165:Luminol; D008297:Male; D012907:Smoking; D013482:Superoxide Dismutase / Q000378:metabolism*; D011795:Surveys and Questionnaires; D013666:Tears / Q000737:chemistry* / Q000201:enzymology
D000975:Antioxidants; D008165:Luminol; D002374:Catalase; D013482:Superoxide Dismutase