title: Disulfide trapping the mechanosensitive channel MscL into a gating-transition state.
abstract: The mechanosensitive channel of large conductance, MscL, serves as a biological emergency release valve protecting bacteria from acute osmotic downshock, and is to date the best characterized mechanosensitive channel. The N-terminal region of the protein has been shown to be critical for function by random, site-directed, and deletion mutagenesis, yet is structurally poorly understood. One model proposes that the extreme N-termini form a cluster of amphipathic helices that serves as a cytoplasmic second gate, separated from the pore-forming transmembrane domain by a "linker". Here, we have utilized cysteine trapping of single-cysteine mutated channels to determine the proximity, within the homopentameric complex, of residues within and just peripheral to this proposed linker. Our results indicate that all residues in this region can form disulfide bridges, and that the percentage of dimers increases when the channel is gated in vivo. Functional studies suggest that oxidation traps one of these mutated channels, N15C, into a gating-transition state that retains the capacity to obtain both fully open and closed states. The data are not easily explained by current models for the smooth transition from closed-to-open states, but predict that an asymmetric movement of one or more of the subunits commonly occurs upon gating.
authors.affiliation: Department of Physiology, University of Texas-Southwestern Medical Center, Dallas, Texas 75390-9040, USA.
authors.forename: Irene
authors.initials: I
authors.lastname: Iscla
authors.forename: Gal
authors.initials: G
authors.lastname: Levin
authors.forename: Robin
authors.initials: R
authors.lastname: Wray
authors.forename: Paul
authors.initials: P
authors.lastname: Blount
mesh_terms: D003545:Cysteine / Q000737:chemistry* / Q000235:genetics; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D015640:Ion Channel Gating; D007473:Ion Channels / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D040542:Mechanotransduction, Cellular; D008958:Models, Molecular; D009154:Mutation; D009997:Osmotic Pressure; D010084:Oxidation-Reduction; D018408:Patch-Clamp Techniques
chemical_list: D004220:Disulfides; D029968:Escherichia coli Proteins; D007473:Ion Channels; C086596:MscL protein, E coli; D003545:Cysteine