Substrate-inducible versions of internal alternative NADH: ubiquinone oxidoreductase from Yarrowia lipolytica.
In standard laboratory strains of the obligate aerobic yeast Yarrowia lipolytica, respiratory chain complex I (proton-translocating NADH : ubiquinone oxidoreductase) is an essential enzyme, since alternative NADH dehydrogenase activity is located exclusively at the external face of the mitochondrial inner membrane. Deletions and other loss-of-function mutations in genes for nuclear coded subunits of complex I can be obtained only when an internal version of the latter enzyme, termed NDH2i, is introduced. In contrast to recent findings with Neurospora crassa, external alternative NADH dehydrogenase activity is dispensable in complex I deletion strains of Y. lipolytica. We used regulable promoters to create strains which express internal alternative NADH dehydrogenase in a substrate-dependent manner. The ability to switch between complex I-dependent and -independent mode of growth simply by changing the carbon source is an important prerequisite for screens for both loss-of-function and inhibitor resistance mutation. The isocitrate lyase promoter (pICL1), in combination with a NDH2i allele that results in reduced expression and activity, was most promising. In the presence of complex I inhibitors, this construct allowed growth on acetate, but not on glucose minimal media. A somewhat higher background was observed with the acyl-CoA oxidase 2 (pPOX2) promoter on glucose minimal media.
Garofano
Aurelio
A
Universität Frankfurt, Fachbereich Medizin, Zentrum der Biologischen Chemie, Theodor-Stern-Kai 7, Haus 26, 60590 Frankfurt am Main, Germany.
Eschemann
Andrea
A
Brandt
Ulrich
U
Kerscher
Stefan
S
D003470:Culture Media / Q000737:chemistry; D042967:Electron Transport Complex I / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D004790:Enzyme Induction / Q000235:genetics; D007522:Isocitrate Lyase / Q000235:genetics; D008928:Mitochondria / Q000201:enzymology; D009245:NADH Dehydrogenase / Q000235:genetics; D011401:Promoter Regions, Genetic; D025062:Yarrowia / Q000201:enzymology* / Q000235:genetics*
D003470:Culture Media; D009245:NADH Dehydrogenase; D007522:Isocitrate Lyase; D042967:Electron Transport Complex I