Effects of azadirachtin on six inorganic cation distributions in Ostrinia furnacalis (G.).
Azadirachtin (Az), as a botanical insecticide, is relatively safe and biodegradable. It affects a wide variety of biological processes, including the reduction of feeding, suspension of molting, death of larvae and pupae, and sterility of emerged adults in a dose-dependent manner. However,the mode of action of this toxin remains obscure. By using ion chromatography, we analyzed changes in six inorganic cation (Li+, Na+, NH4+, K+, Mg2+, and Ca2+) distributions of the whole body and hemolymph in Ostrinia furnacalis (G.) after exposure to sublethal doses of Az. The results showed that Az dramatically interfered with Na+, NH4 +, K+, Mg2+, and Ca2+ distributions in hemolymph of O. furnacalis (G.) and concentrations of these five cations dramatically increased. However, in the whole body, the levels of K+, Mg2+, and Ca2+ significantly, decreased after exposure to Az, except that Na+ and NH4 + remained constant. Li+ was undetected in both the control and treated groups in the whole body and hemolymph. It is suggested that Az exerts its insecticidal effects on O. furnacalis (G.) by interfering with the inorganic cation distributions related to ion channels.
Shi
Ping
P
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, People's Republic of China.
Huang
Zhiwei
Z
Chen
Guichen
G
Zhou
Li
L
Tan
Xiaorong
X
D000641:Ammonia / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002412:Cations / Q000378:metabolism*; D006458:Hemolymph / Q000378:metabolism; D007915:Lepidoptera / Q000378:metabolism*; D036701:Limonins / Q000494:pharmacology*; D008094:Lithium / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D014018:Tissue Distribution
D002412:Cations; D036701:Limonins; D000641:Ammonia; D008094:Lithium; D012964:Sodium; D008274:Magnesium; C010329:azadirachtin; D011188:Potassium; D002118:Calcium