title: Is HLA class II profile relevant for the study of large-scale differentially expressed genes in type 1 diabetes mellitus patients?
abstract: We have previously identified 30 differentially expressed genes when comparing recently diagnosed type 1 diabetes mellitus (DM-1) patients and controls paired for sex, age, and ethnic background. In this article we performed the hierarchical clustering of these genes taking into account the human-leukocyte-antigen (HLA)-DRB1/DQB1 profile. The dendrogram obtained using the Cluster program grouped patients and controls into three clusters, one including individuals with no susceptibility alleles, another including individuals with at least three susceptibility alleles, and a third intermingling susceptibility/protective alleles. In addition to other variables, the results of the present article suggest that the major histocompatibility complex (MHC) class II profile may be of relevance for the study of a large-scale differentially expressed genes.
authors.affiliation: Divisão de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo-USP, 14049-900 São Paulo, S.P., Brazil.
authors.forename: Diane M
authors.initials: DM
authors.lastname: Rassi
authors.forename: Cristina M
authors.initials: CM
authors.lastname: Junta
authors.forename: Ana L
authors.initials: AL
authors.lastname: Fachin
authors.forename: Paula
authors.initials: P
authors.lastname: Sandrin-Garcia
authors.forename: Stephano S
authors.initials: SS
authors.lastname: Mello
authors.forename: Ana P M
authors.initials: AP
authors.lastname: Fernandes
authors.forename: Neife N H S
authors.initials: NN
authors.lastname: Deghaide
authors.forename: Maria C
authors.initials: MC
authors.lastname: Foss-Freitas
authors.forename: Milton C
authors.initials: MC
authors.lastname: Foss
authors.forename: Elza T
authors.initials: ET
authors.lastname: Sakamoto-Hojo
authors.forename: Geraldo A S
authors.initials: GA
authors.lastname: Passos
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Donadi
mesh_terms: D000465:Algorithms; D000483:Alleles; D016022:Case-Control Studies; D002648:Child; D016000:Cluster Analysis; D003922:Diabetes Mellitus, Type 1 / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D005260:Female; D015870:Gene Expression; D006683:HLA-DQ Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D008297:Male; D008660:Metabolism / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II