Improved yields of full-length functional human FGF1 can be achieved using the methylotrophic yeast Pichia pastoris.
We have produced human fibroblast growth factor 1 (hFGF1) in the methylotrophic yeast Pichia pastoris in order to obtain the large amounts of active protein required for subsequent functional and structural characterization. Four constructs were made to examine both intracellular and secreted expression, with variations in the location of the His6 tag at either end of the peptide. hFGF1 could be produced from all four constructs in shake flasks, but production was optimized by growing only the highest-yielding of these strains, which produced hFGF1 intracellularly, under tightly controlled conditions in a 3 L fermentor. One hundred and eight milligrams of pure protein was achieved per liter culture (corresponding to 0.68 mg of protein per gram of wet cells), the function of which was verified using NIH 3T3 cell cultures. This is a 30-fold improvement over previously reported yields of full-length hFGF1.
Fantoni
Adele
A
Magnetic Resonance Center (Centro di Risonanze Magnetiche), University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy.
Bill
Roslyn M
RM
Gustafsson
Lena
L
Hedfalk
Kristina
K
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D016220:Fibroblast Growth Factor 1 / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D051379:Mice; D010843:Pichia / Q000254:growth & development / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology
D017931:DNA Primers; D018076:DNA, Complementary; D011994:Recombinant Proteins; D016220:Fibroblast Growth Factor 1