HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum.
A sensitive and simple high-performance liquid chromatography (HPLC) method was applied for the quantitative determination of four major triterpenoids (ganoderic acids C(2), B, K and H) in rat urine after oral administration of total triterpenoids from Ganoderma lucidum. The urine sample was extracted with dichloromethane-ethyl acetate (90:10) after acidification by hydrochloric acid (0.2 mol/ml). Chromatographic separation was achieved on a Zorbax SB-C(18) column (250 mm x 4.6 mm, 5 microm) at 35 degrees C, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.2 ml/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. The within- and between-day assay coefficients of variation for the four triterpenoids in urine were less than 9% and the extraction recovery of this method was higher than 90%. Using this method, the excretion profile of the triterpenoids in rat urine after oral administration of total triterpenoids of G. lucidum was revealed for the first time.
Wang
Xiao-Ming
XM
Shanghai Research Center for TCM Modernization, Shanghai Institute of Materia Medica, Shanghai Institute for Biological Sciences, Guo Shoujing Road 199, Zhangjiang, Shanghai, PR China.
Guan
Shu-Hong
SH
Liu
Rong-Xia
RX
Sun
Jiang-Hao
JH
Liang
Yan
Y
Yang
Min
M
Wang
Wei
W
Bi
Kai-Shun
KS
Guo
De-An
DA
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D020940:Reishi / Q000737:chemistry*; D015203:Reproducibility of Results; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014315:Triterpenes / Q000493:pharmacokinetics / Q000652:urine*
D007202:Indicators and Reagents; D014315:Triterpenes