title: Single-nucleotide polymorphism frequency in a set of selected lines of bread wheat (Triticum aestivum L.).
abstract: Information on single-nucleotide polymorphisms (SNPs) in hexaploid bread wheat is still scarce. The goal of this study was to detect SNPs in wheat and examine their frequency. Twenty-six bread wheat lines from different origins worldwide were used. Specific PCR-products were obtained from 21 genes and directly sequenced. SNPs were discovered from the alignment of these sequences. The overall sequence polymorphism observed in this sample appears to be low; 64 single-base polymorphisms were detected in approximately 21.5 kb (i.e., 1 SNP every 335 bp). The level of polymorphism is highly variable among the different genes studied. Fifty percent of the genes studied contained no sequence polymorphism, whereas most SNPs detected were located in only 2 genes. As expected, taking into account a synthetic line created with a wild Triticum tauschii parent increases the level of polymorphism (101 SNPs; 1 SNP every 212 bp). The detected SNPs are available at http://urgi.versailles.inra.fr/GnpSNP">http://urgi.versailles.inra.fr/GnpSNP. Data on linkage disequilibrium (LD) are still preliminary. They showed a significant level of LD in the 2 most polymorphic genes. To conclude, the genome size of hexaploid wheat and its low level of polymorphism complicate SNP discovery in this species.
authors.affiliation: INRA, UMR1095, Amélioration et Santé des Plantes, 234 avenue du Bézet, Clermont-Ferrand, F-63100 France. ravel@clermont.inra.fr
authors.forename: Catherine
authors.initials: C
authors.lastname: Ravel
authors.forename: Sébastien
authors.initials: S
authors.lastname: Praud
authors.forename: Alain
authors.initials: A
authors.lastname: Murigneux
authors.forename: Aurélie
authors.initials: A
authors.lastname: Canaguier
authors.forename: Frédéric
authors.initials: F
authors.lastname: Sapet
authors.forename: Delphine
authors.initials: D
authors.lastname: Samson
authors.forename: François
authors.initials: F
authors.lastname: Balfourier
authors.forename: Philippe
authors.initials: P
authors.lastname: Dufour
authors.forename: Boulos
authors.initials: B
authors.lastname: Chalhoub
authors.forename: Dominique
authors.initials: D
authors.lastname: Brunel
authors.forename: Michel
authors.initials: M
authors.lastname: Beckert
authors.forename: Gilles
authors.initials: G
authors.lastname: Charmet
mesh_terms: D001939:Bread; D030561:Databases, Nucleic Acid; D017353:Gene Deletion; D005787:Gene Frequency; D018745:Genome, Plant; D006239:Haplotypes; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide; D011123:Polyploidy; D014908:Triticum / Q000235:genetics*