Impaired platelet mitochondrial activity in Alzheimer's disease and mild cognitive impairment.
Mitochondrial abnormalities are found in Alzheimer's disease (AD), but previous reports have not examined at-risk groups. In subjects with AD, mild cognitive impairment (MCI), and non-demented aged controls, platelet and lymphocyte mitochondria were isolated and analyzed for Complexes I, III, and IV of the electron transport chain. Western blots were used to control for differential enrichment of samples. Results demonstrated significant declines in Complexes III and IV in AD, and a significant decline in Complex IV in MCI. This report confirms mitochondrial deficiencies in AD, extends them to MCI, and suggests they are present at the earliest symptomatic stages of disease.
Valla
Jon
J
Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Arizona Alzheimer's Disease Consortium, 350 W. Thomas Road, Phoenix, AZ 85013, USA. jvalla@chw.edu
Schneider
Lonnie
L
Niedzielko
Tracy
T
Coon
Keith D
KD
Caselli
Richard
R
Sabbagh
Marwan N
MN
Ahern
Geoffrey L
GL
Baxter
Leslie
L
Alexander
Gene
G
Walker
Douglas G
DG
Reiman
Eric M
EM
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology*; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism*; D003072:Cognition Disorders / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology