title: A liquid chromatography-mass spectrometry method for the quantitation of aristololactam-I in rat plasma.
abstract: A sensitive method with liquid chromatography-electrospray ionization mass spectrometry has been developed and validated for the determination of aristololactam-I in rat plasma after oral administration of aristolochic acid-I using finesteride as the internal standard. Chromatographic separation was achieved on a Lichrospher C(18) column using methanol:0.05% acetic acid in water (71:29, v/v) as a mobile phase delivered at a flow rate of 1 mL/min. The assay was linear for aristololactam-I over the range 0.3-300 ng/mL. The analysis of quality control samples demonstrated precision with coefficient of variation less than 20% (n = 5). Absolute recovery of aristololactam-I was 90.4-97.3%. The LC-MS method for the determination of aristololactam-I is sensitive, specific and can be used to investigate the toxicokinetics of aristololactam-I.
authors.affiliation: New Drug Screen Centre, China Pharmaceutical University, Nanjing, 210038, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ling
authors.forename: Zhen-zhou
authors.initials: ZZ
authors.lastname: Jiang
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Lu-yong
authors.initials: LY
authors.lastname: Zhang
authors.forename: Xiao-yue
authors.initials: XY
authors.lastname: Xu
mesh_terms: D000818:Animals; D034341:Aristolochic Acids / Q000097:blood* / Q000633:toxicity; D002138:Calibration; D002853:Chromatography, Liquid; D008297:Male; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D034341:Aristolochic Acids; C121135:aristolactam I