[Encapsulating hepatocytes with chitosan in physiological conditions].
Prepared from 15.3% N-acetylated chitosan (FNC), half N-acetylated chitosan (HNC) possesses a good solubility in a weak basic solution, guaranteeing the formation of microcapsules by the coacervating reaction between HNC and methacrylic acid (MAA)-hydroxyethyl methacrylate (HEMA)-methyl methacrylate (MMA) (MAA-HEMA-MMA) terpolymer under physiological conditions. When hepatocytes were encapsulated in such 3-dimensional microenvironment, as compared to monolayer culture, cell functions, including P450 activity, urea production and albumin release, were well supported. The prepared microcapsules have good mechanical stability and permeability.
Zhu
Jianhang
J
Department of Chemical Engineering, Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330029, China. envzjh@ncu.edu.cn
Zhang
Bao
B
Yan
Xiluan
X
Lao
Xuejun
X
Yu
Hanry
H
D000818:Animals; D002214:Capsules; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008689:Methacrylates; D020366:Methylmethacrylate; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods
D002214:Capsules; D008689:Methacrylates; D011108:Polymers; D020366:Methylmethacrylate; C008384:methacrylic acid; C005044:hydroxyethyl methacrylate; D048271:Chitosan