title: Subtractive hybridization magnetic bead capture: a new technique for the recovery of full-length ORFs from the metagenome.
abstract: A new method for the recovery of full-length open reading frames from metagenomic nucleic acid samples is reported. This technique, based on subtractive hybridization magnetic bead capture technology, has the potential to access multiple gene variants from a single amplification reaction. It is now widely accepted that classical microbiological methods provide only limited access to the true microbial biodiversity (less than 1%). The desire to access a higher proportion of the metagenome has led to the development of efficient environmental nucleic acid extraction technologies and to a range of sequence-dependent and sequence-independent gene discovery techniques. These methods avoid many of the limitations of culture-dependent gene targeting.
authors.affiliation: Advanced Research Centre for Applied Microbiology, Department of Biotechnology, University of the Western Cape, Cape Town, South Africa.
authors.forename: Quinton C
authors.initials: QC
authors.lastname: Meyer
authors.forename: Stephanie G
authors.initials: SG
authors.lastname: Burton
authors.forename: Don A
authors.initials: DA
authors.lastname: Cowan
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D018390:Gene Targeting / Q000379:methods; D023281:Genomics / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods*; D008280:Magnetics; D008863:Microspheres; D016366:Open Reading Frames / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA