title: [Study on interaction between sulfonylurea herbicides and catalase by fluorescence spectroscopy].
abstract: The binding of Sulfonylurea herbicides to catalase in aqueous solution was studied using fluorescence spectroscopy. It was shown that herbicides have a strong ability to quench the catalase fluorescence mainly through a static quenching procedure. The binding constant K and the number of binding site n were calculated according to the fluorescence quenching results. For chlorsufuron, K=8.69 x 10(5) L x mol(-1) and n = 1.16; for metsufuron methyl, K = 1.01 x 10(6) L x mol(-1) and n = 1.21; and for bensufuron methyl, K = 3.52 x 10(3) L x mol(-1), n = 0.77. It is clear that the binding of metsufuron methyl with catalase is stronger than that of chlorsufuron, while the binding of chlorsufuron stronger than that of bensufuron methyl.
authors.affiliation: Department of Chemistry, College of Science, Huazhong Agriculture University, Wuhan 430070, China.
authors.forename: Fa-Bing
authors.initials: FB
authors.lastname: Ye
authors.forename: Yuan-Yan
authors.initials: YY
authors.lastname: Dong
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Xiao-Man
authors.initials: XM
authors.lastname: Mo
authors.forename: Xian-Wen
authors.initials: XW
authors.lastname: Hu
mesh_terms: D000465:Algorithms; D001190:Arylsulfonates / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D002374:Catalase / Q000737:chemistry* / Q000378:metabolism; D005453:Fluorescence; D006540:Herbicides / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013453:Sulfonylurea Compounds / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001190:Arylsulfonates; D006540:Herbicides; D013453:Sulfonylurea Compounds; C050296:metsulfuron methyl; D002374:Catalase