Successful RNA extraction from various human postmortem tissues.
Recently, several authors described the observation that RNA degradation does not correlate with the postmortem interval (PMI), but rather with other parameters like environmental impact and the circumstances of death. Therefore, the question arose if the analysis of gene expression could be a valuable tool in forensic genetics to contribute to the determination of the cause of death. In our study, six human tissues obtained from six individuals with PMI varying between 15 and 118 h were used for total RNA extraction. Quantification was performed using a GAPDH real-time assay, and the quality of mRNA was checked by amplification of different fragment lengths of the GAPDH transcript. In our set of samples, nearly all tissues in all PMI revealed satisfactory results, while skeletal muscle, followed by brain and heart, gave the best results. No correlation between PMI and RNA degradation could be detected, as very good results were observed for all tissues from the individual with the longest PMI. The highly promising results obtained in this study raise hopes that in the near future several fields of forensic investigation may profit from additional information about gene expression patterns and their correlation with pathological findings.
Heinrich
Marielle
M
Institute of Legal Medicine, University of Freiburg, Albertstr. 9, 79104, Freiburg, Germany. marielle.heinrich@uniklinik-freiburg.de
Matt
Katja
K
Lutz-Bonengel
Sabine
S
Schmidt
Ulrike
U
D001344:Autopsy / Q000379:methods*; D002423:Cause of Death; D020869:Gene Expression Profiling / Q000379:methods*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011180:Postmortem Changes; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013997:Time Factors; D014018:Tissue Distribution
D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases