Immunostimulating effects of the polyphenol-rich fraction of sugar cane (Saccharum officinarum L.) extract in chickens.
The phagocytic activity of peripheral blood leukocytes (PBL) in chickens orally administered sugar cane extracts (SCE) or polyphenol-rich fraction (PRF) of SCE (500 mg/kg/day) for 3 consecutive days increased significantly, when compared with that of saline-administered control chickens. Chickens orally administered SCE or PRF (500 mg/kg/day) for 3 consecutive days showed significantly higher antibody responses against sheep red blood cells and Brucella abortus than control chickens. In addition, oral administration of SCE or PRF also resulted in a significant increase in the number of IgM- and IgG-plaque forming cell responses of PBL, intestinal leukocytes and splenocytes, when compared with those of control chickens. Furthermore, delayed type hypersensitivity responses to human gamma globulin significantly increased in chickens orally administered SCE or PRF, compared with those of control chickens when evaluated on the basis of net increased wattle thickness at 24, 48 and 72 h after challenge. These results suggest that PRF of SCE has an immunostimulating effect in chickens.
Hikosaka
Kenji
K
Department of Parasitology, National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan.
El-Abasy
Moshira
M
Koyama
Yukari
Y
Motobu
Maki
M
Koge
Kenji
K
Isobe
Takashi
T
Kang
Chung-Boo
CB
Hayashidani
Hideki
H
Onodera
Takashi
T
Wang
Pi-Chao
PC
Matsumura
Masatoshi
M
Hirota
Yoshikazu
Y
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002003:Brucella abortus / Q000502:physiology; D002645:Chickens / Q000276:immunology* / Q000382:microbiology; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D059808:Polyphenols; D031786:Saccharum / Q000737:chemistry*; D012756:Sheep / Q000097:blood
D000276:Adjuvants, Immunologic; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols