title: Production of scFv-Fc fusion protein using genetically manipulated quails.
abstract: The use of transgenic avian species as a transgenic bioreactor for the production of recombinant proteins has been proposed. In recent years, although various procedures for generating transgenic chickens have been reported, the expression of a useful protein at a commercially feasible level has rarely been attained. In this study, we injected a concentrated retroviral vector into quail embryos to generate genetically manipulated quails that produce recombinant proteins. We found that transgene expression in the whole body at a high level was observed for viral injection into the heart of the developing embryos after a 48-h incubation. For the practical production of a useful protein, a retroviral vector encoding an anti-prion scFv-Fc gene under the control of the beta-actin promoter was injected into quail embryos. The quails that hatched stably produced scFv-Fc at a high level in their serum and egg white. The production of scFv-Fc was maintained throughout the breeding period. scFv-Fc purified from the egg white retained the antigen-binding activity. This system exhibited the potential of transgenic quails for the commercial production of recombinant proteins.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kawabe
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kamihira
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Ono
authors.forename: Kenji
authors.initials: K
authors.lastname: Kyogoku
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Nishijima
authors.forename: Shinji
authors.initials: S
authors.lastname: Iijima
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D003370:Coturnix / Q000235:genetics*; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism*; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
chemical_list: D007141:Immunoglobulin Fc Fragments; D007135:Immunoglobulin Variable Region; D011993:Recombinant Fusion Proteins