Studies on novel bacterial translocase I inhibitors, A-500359s. V. Enhanced production of capuramycin and A-500359 A in Streptomyces griseus SANK 60196.
Streptomyces griseus SANK 60196 produces the novel nucleoside antibiotics A-500359 A, C, D and capuramycin. Enhanced production of capuramycin and A-500359 A was achieved through a number of medium modifications and a series of single colony isolations. The addition of maltose instead of glucose as the carbon source in a primary medium resulted in a 20-fold increase in the productivity of capuramycin. Furthermore, the addition of cobalt chloride (CoCl2) and yeast extract to the medium containing maltose drastically altered the production ratio of A-500359 A to capuramycin. Thus, the yield of A-500359 A increased up to 600 microg/ml in an optimal medium, while the yield in the primary medium was 1 microg/ml.
Muramatsu
Yasunori
Y
Lead Discovery Research Laboratories, Sankyo Co., Ltd., 1-2-58 Hiromachi, Shinagawa-ku, Tokyo 140-8710, Japan. yasuno@sankyo.co.jp
Arai
Masatoshi
M
Sakaida
Yoshiharu
Y
Takamatsu
Yasuyuki
Y
Miyakoshi
Shunichi
S
Inukai
Masatoshi
M
D000617:Aminoglycosides / Q000096:biosynthesis*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003035:Cobalt / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D009705:Nucleosides / Q000096:biosynthesis*; D013305:Streptomyces griseus / Q000378:metabolism*
C515842:A-500359 A; C515841:A-500359 C; C515843:A-500359 D; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D003470:Culture Media; D004791:Enzyme Inhibitors; D009705:Nucleosides; C049983:capuramycin; D003035:Cobalt; D008320:Maltose; C018021:cobaltous chloride; D005947:Glucose