Detection of Rickettsia rickettsii and Rickettsia sp. in blood clots in 24 patients from different municipalities of the State of Sao Paulo, Brazil.
The authors detected Rickettsia genus organisms using shell vial and polymerase chain reaction (PCR)/sequencing analysis in blood clots in patients suspected of having Brazilian spotted fever (BSF). DNA was detected using PCR with three sets of primers to access the gltA, ompA, and ompB genes. Sequence analysis was carried out using an automatic sequencer with Bioedit software. Seventy-five percent of the culture samples were positive and all samples amplified rickettsial gene fragments. To date, 46% of the samples have been sequenced.
Gehrke
Flávia Sousa
FS
Instituto de Ciências Biomédicas II, Av. Prof. Lineu Prestes, 1374, Cidade Universtitária, 05508-900 São Paulo, Brazil. fgehrke@usp.br
Mendes do Nascimento
Elvira Maria
EM
Rodrigues de Souza
Eliana
E
Colombo
Silvia
S
Jacintho da Silva
Luiz
L
Schumaker
Teresinha Tizu Sato
TT
D001777:Blood Coagulation; D001938:Brazil / Q000453:epidemiology; D017931:DNA Primers; D006801:Humans; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012281:Rickettsia / Q000235:genetics / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000097:blood / Q000453:epidemiology*; D012284:Rickettsia rickettsii / Q000235:genetics / Q000302:isolation & purification*; D012373:Rocky Mountain Spotted Fever / Q000097:blood / Q000453:epidemiology
D017931:DNA Primers