12(S)-HPETE induces itch-associated scratchings in mice.
The itch-associated responses evoked by intradermal injection of 12(S)-HPETE and leukotriene B4 were compared in ICR-mice. 12(S)-HPETE and leukotriene B4 (0.01-0.2 nmol/site) induced scratching of the injected site, respectively; the dose-responses were a peak at 0.05 nmol/site (12(S)-HPETE) or 0.03 nmol/site (leukotriene B4). The scratching response by 12(S)-HPETE (0.05 nmol/site) started within 1 min, peaked in the first 10 min period, had almost subsided by 25 min whereas the effect of leukotriene B4 peaked in the second 10 min. The effect of leukotriene B4 is slightly stronger than that of 12(S)-HPETE in 40 min of count. The scratching induced by 12(S)-HPETE was inhibited by capsaicin, naltrexon, and LY255283. These results suggest the possibility that 12-lipoxygenase product can be added to a new member of an endogenous itch mediator in the skin.
Kim
Dae-Kwon
DK
Dermatologic Drug Research, AmorePacific Corporation R&D Center, Yongin-si, Gyeonggi-do, 449-729, Republic of Korea.
Kim
Hyung-June
HJ
Sung
Ki-Sa
KS
Kim
Hyuk
H
Cho
Sun-A
SA
Kim
Kwang-Mi
KM
Lee
Chang-Hoon
CH
Kim
Jung-Ju
JJ
D000818:Animals; D002211:Capsaicin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007975:Leukotriene B4 / Q000494:pharmacology; D015289:Leukotrienes / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009271:Naltrexone / Q000494:pharmacology; D011537:Pruritus / Q000139:chemically induced*; D018102:Receptors, Leukotriene B4 / Q000037:antagonists & inhibitors
D015289:Leukotrienes; D018102:Receptors, Leukotriene B4; D007975:Leukotriene B4; D009271:Naltrexone; C021201:12-HPETE; D002211:Capsaicin