title: Constituents of the roots of Pueraria lobata inhibit formation of advanced glycation end products (AGEs).
abstract: Two isoflavone C-glucosides, puerarin (1) and PG-3 (2), a but-2-enolide, (+/-)-puerol B (3), two isoflavone O-glucosides, daidzin (4) and genistin (5), and three pterocarpans, (-)-medicarpin (6), (-)-glycinol (7) and (-)-tuberosin (8), were isolated from a MeOH extract of the roots of Pueraria lobata, using an in vitro bioassay based on the inhibition of the formation of advanced glycation end products (AGEs) to monitor chromatographic fractionation. The structures of 1-8 were determined by spectroscopic data interpretation, particularly by 1D- and 2D-NMR studies, and by comparison of these data with values in the literature. All of the isolates (1-8) were evaluated for their inhibitory activity on AGEs formation in vitro. Of these, puerarin (1), PG-3 (2), and (+/-)-puerol B (3) exhibited more potent inhibitory activity than the positive control aminoguanidine.
authors.affiliation: Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Yun Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Ga Young
authors.initials: GY
authors.lastname: Lee
authors.forename: Dae Sik
authors.initials: DS
authors.lastname: Jang
authors.forename: Ki Hwan
authors.initials: KH
authors.lastname: Bae
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Kim
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods
chemical_list: C517305:3'-methoxypuerarin; D005960:Glucosides; D017127:Glycation End Products, Advanced; D007529:Isoflavones; D010636:Phenols; D010936:Plant Extracts; C517304:puerol B; D015107:4-Butyrolactone; C033607:puerarin