[Changes of matrix metalloproteinases activities in pulmonary fibrosis rats].
OBJECTIVE
To observe the changes of matrix metalloproteinases (MMPs) activities in pulmonary fibrosis rats.


METHODS
Eighty male SD rats were randomly divided into sham group (n = 40) and bleomycin group (BLM, n = 40), in which SD rats were injected with a single intratracheal dose of sham saline or bleomycin respectively. On day 1, 3, 7, 14, and 28 following bleomycin or saline instillation, rats were randomly killed, and serum from abdominal aorta, alveolar fluid from the bronchoalveolar lavage, and the lung homogenate were collected and then stored at -80 degrees C. MMPs activity was determined by zymography.


RESULTS
Compared with sham group, the levels of MMP-9 in all samples were augmented. MMP-9 activities in the serum were highest on day 3 than those on day 1 and day 7, and in lung tissue homogenate were highest on day 7; however, no significant differences were found between BLM group and sham group on day 14 and day 28; and that of bronchoalveolar lavage fluid (BALF) was highest on day 7 than those on day 1 and day 14, while no significant difference existed between BLM group and sham group on day 28. Serum MMP-2 level did not change from day 1 to day 28, while the level of BALF MMP-2 began to increase after day 14, even on day 28. Lung tissue homogenate MMP-2 level began to increase early on day 3 and continued to day 28.


CONCLUSIONS
The sources and effects of MMP-2 and MMP-9 differ in BLM-induced rat pulmonary fibrosis.
Kong
Lu
L
Laboratory of Biochemistry and Molecular Biology, Capital Medical University, Beijing 100069, China. kongluxinxiang@sina.com.cn
Pan
Ying
Y
Yu
He-fen
HF
Wang
Ji-feng
JF
Niu
Jian-zhao
JZ
D000818:Animals; D001761:Bleomycin / Q000633:toxicity; D004195:Disease Models, Animal; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001761:Bleomycin; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9