title: Inhibition of HIV-1 replication by an improved hairpin ribozyme that includes an RNA decoy.
abstract: An anti-Tat hairpin ribozyme and a TAR RNA decoy were combined in one molecule. The chimeric molecule strongly inhibited HIV-1 replication (measured as changes in p24 levels in viral replication assays). The inhibitory action of the ribodecozyme (85%) was significantly greater than that shown by ribozyme and a non-catalytic variant carrying the functional decoy RNA domain (55% and 35%, respectively). This represents a significant improvement of the inhibitory efficiency of the ribozyme, suggesting there is an additive inhibitory effect on HIV-1 replication by the catalytic and decoy domains. This strategy could be used to create new inhibitor RNAs with enhanced in vivo performance.
authors.affiliation: Instituto de Parasitología y Biomedicina López-Neyra, CSIC, Parque Tecnológico de Ciencias de la Salud, Avda, del Conocimiento s/n, Granada, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Barroso-DelJesus
authors.forename: Elena
authors.initials: E
authors.lastname: Puerta-Fernández
authors.forename: Natalia
authors.initials: N
authors.lastname: Tapia
authors.forename: Cristina
authors.initials: C
authors.lastname: Romero-López
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Sánchez-Luque
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Martínez
authors.forename: Alfredo
authors.initials: A
authors.lastname: Berzal-Herranz
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D015195:Drug Design; D015696:Gene Products, tat / Q000037:antagonists & inhibitors; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D016337:RNA, Catalytic / Q000494:pharmacology*; D012367:RNA, Viral / Q000037:antagonists & inhibitors*; D014779:Virus Replication / Q000187:drug effects*; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D015696:Gene Products, tat; D016337:RNA, Catalytic; D012367:RNA, Viral; C418481:hairpin ribozyme; D054322:tat Gene Products, Human Immunodeficiency Virus