Assay of glutathione in must and wines using capillary electrophoresis and laser-induced fluorescence detection. Changes in concentration in dry white wines during alcoholic fermentation and aging.
Glutathione (GSH) was assayed in must and wine using capillary electrophoresis coupled with laser-induced fluorescence (LIF) detection. Sample preparation involved conjugating thiols with monobromobimane (MBB) in a 2-(N-cyclohexylamino)ethanesulfonic acid [CHES] buffer (179mM). The electrophoretic conditions were 30kV with a capillary length of 105cm from the inlet to the detector (120cm total length) and a 50microm inner diameter. Under these conditions, the complete separation from the other main non-volatile thiols took less than 20min. We also described the optimum conditions for derivatizing wine samples with MBB to increase eletrophoretic sensitivity. The detection limit for glutathione assay is 65nmol/L. This simple, sensitive method provides a specific assay of glutathione in reduced form, as the sample preparation technique does not modify the balance of oxidized and reduced forms. We used this method to monitor changes in the reduced glutathione content of a white wine during alcoholic fermentation and barrel aging.
Lavigne
Valérie
V
SEGUIN MOREAU France, Z.I. Merpins, B.P. 94, 16103 Cognac, France.
Pons
Alexandre
A
Dubourdieu
Denis
D
D001643:Bridged Bicyclo Compounds / Q000032:analysis / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005978:Glutathione / Q000032:analysis* / Q000737:chemistry; D007834:Lasers; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors; D014920:Wine / Q000032:analysis*
D001643:Bridged Bicyclo Compounds; D000431:Ethanol; D005978:Glutathione; C028381:monobromobimane