Micellanized spectrophotometric method for the determination of beryllium using haematoxylin.
A simple and sensitized spectrophotometric method for the determination of trace amounts of beryllium has been described. The method is based on the formation of a ternary complex by the reaction of beryllium with haematoxylin in the presence of micellar medium (cationic surfactant, cetyltrimethylammonium bromide). The ternary complex of beryllium has a maximum absorbance at 592 nm and showed an excellent sensitivity (molar absorption coefficient of 7.07 x 10(4)L mol(-1)cm(-1) and the Sandell's sensitivity being 1.27 x 10(-4) microg cm(-2)) and reproducibility (within-day precision: R.S.D.<or=0.36%, n=5, between-day precision: R.S.D.<or=0.65%, n=5). Linearity was achieved over the range 0.01-0.1 microg mL(-1) of beryllium with a correlation coefficient of 0.9999. The effect of foreign substances on the determination has been examined. The proposed procedure has been applied to the determination of beryllium in water samples.
Dayananda
B P
BP
Department of Chemistry, University of Mysore, Manasagangothri, Mysore 570006, India.
Revanasiddappa
H D
HD
Kiran Kumar
T N
TN
D001608:Beryllium / Q000032:analysis*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D006416:Hematoxylin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D013053:Spectrophotometry / Q000379:methods*; D013501:Surface-Active Agents / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry
D002593:Cetrimonium Compounds; D008823:Micelles; D013501:Surface-Active Agents; D014867:Water; D001608:Beryllium; D006416:Hematoxylin; D000077286:Cetrimonium