Sequence analysis of a novel insertion site of transposon IS10.
A sacB mutant was obtained by transposon IS10 inactivation of a plasmid pXT3sacB carrying the sacB gene. Sequencing of this mutant plasmid DNA (GenBank accession No. AY580883.1) showed that the IS10 flanking the 22 bp inverted repeats were 5'-CTGAGAGATCCCCTCATAATTT-3' and 5'-AAATCATTAGGGGATTCATCAG-3', which were the similar to those published in reports previously. However, the target sequence adjacent to IS10 was 5'-TGCTTGGTT-3' instead of the previously reported 5'-NGCTNAGCN-3'. To our knowledge, this is the first report on the novel insertion site of IS10. In addition, Southern blot hybridization confirmed that the mobile IS10 originated from the chromosomal DNA of the host strain Escherichia coli DH5alpha and that there were two copies in the DH5alpha genome.
Xiang
Tai-He
TH
School of Life Sciences, Hangzhou Normal College, Hangzhou 310036, China. xthcn@163.com
Wang
Li-Lin
LL
Wang
Hui-Zhong
HZ
D001483:Base Sequence; D015139:Blotting, Southern; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000235:genetics*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes