[Expression of Pin1, beta-catenin and cyclin D1 in salivary adenoid cystic carcinoma and its significance].
OBJECTIVE
To investigate the expression of Pin1, beta-catenin and cyclin D1 in salivary adenoid cystic carcinomas (SACC) and to evaluate the role of beta-catenin and Pin1 in SACC carcinogenesis.


METHODS
The expressions of Pin1, beta-catenin and cyclin D1 were examined in the specimens of 65 patients with SACC by immunohistochemistry, and Pin1 protein and mRNA expressions detected by Western blot and RT-PCR in four SACC cell lines.


RESULTS
Pin1 was overexpressed in 51 cases of ACC (78%), and 41 (63%) cases showed positive immunoreactivity for cyclin D1 protein in the nuclear fraction in tumor tissues. Fourteen (22%) cases showed positive immunoreactivity for beta-catenin protein in the nuclear/cytoplasmic fraction in tumor tissues, 6 of which exhibited quite evident expression of beta-catenin in nucleolus. The expression of membranous beta-catenin was down-regulated in most of the patients with lymph node metastasis (11/14).


CONCLUSIONS
The results suggest that Pin1 and beta-catenin signalling pathway were activated in SACC and might play a pivotal role in SACC carcinogenesis and metastasis.
Zhou
Chuan-xiang
CX
Department of Oral Pathology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Gao
Yan
Y
D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000378:metabolism; D006801:Humans; D008207:Lymphatic Metastasis; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D009361:Neoplasm Invasiveness; D019696:Peptidylprolyl Isomerase / Q000378:metabolism*; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology*; D051176:beta Catenin / Q000378:metabolism*
C530104:CCND1 protein, human; C495270:CTNNB1 protein, human; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D051176:beta Catenin; D019938:Cyclin D1; C000606846:PIN1 protein, human; D019696:Peptidylprolyl Isomerase