title: Circulating tumoral cells lack circadian-rhythm in hospitalized metastasic breast cancer patients.
abstract: BACKGROUND
  The relationship between breast cancer and circadian rhythm variation has been extensively studied. Increased breast tumorigenesis has been reported in melatonin-suppressed experimental models and in observational studies.
  
  
  OBJECTIVE
  Circulating Tumor Cells (CTC) circadian- rhythm may optimize the timing of therapies. This is a prospective experimental study to ascertain the day-time and night-time CTC levels in hospitalized metastasic breast cancer (MBC) patients.
  
  
  METHODS
  CTC are isolated and enumerated from a 08:00 AM and 08:00 PM blood collections. 23 MBC and 23 healthy volunteers entered the study. 69 samples were collected (23 samples at 08:00 AM and 23 samples at 08:00 PM from MBC; 23 samples from healthy volunteers). Results from two patients were rejected due to sample processing errors. No CTC were isolated from healthy-volunteers.
  
  
  CONCLUSIONS
  No-differences between daytime and night-time CTC were observed. Therefore, we could not ascertain CTC circadian-rhythm in hospitalized metastasic breast cancer patients.
authors.affiliation: Servicio de Oncología Médica. Hospital Universitario San Carlos. Madrid. Spain. jagsaenz@yahoo.com
authors.forename: José Angel
authors.initials: JA
authors.lastname: García-Sáenz
authors.forename: Miguel
authors.initials: M
authors.lastname: Martín
authors.forename: Marisa
authors.initials: M
authors.lastname: Maestro
authors.forename: Marta
authors.initials: M
authors.lastname: Vidaurreta
authors.forename: Silvia
authors.initials: S
authors.lastname: Veganzones
authors.forename: Laura
authors.initials: L
authors.lastname: Villalobos
authors.forename: Laura
authors.initials: L
authors.lastname: Rodríguez-Lajusticia
authors.forename: Sara
authors.initials: S
authors.lastname: Rafael
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Sanz-Casla
authors.forename: Antonio
authors.initials: A
authors.lastname: Casado
authors.forename: Javier
authors.initials: J
authors.lastname: Sastre
authors.forename: Manuel
authors.initials: M
authors.lastname: Arroyo
authors.forename: Eduardo
authors.initials: E
authors.lastname: Díaz-Rubio
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use; D018931:Antineoplastic Agents, Hormonal / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000097:blood* / Q000188:drug therapy / Q000473:pathology; D002452:Cell Count; D002453:Cell Cycle; D002940:Circadian Rhythm; D020847:Estrogen Receptor Modulators / Q000627:therapeutic use; D004967:Estrogens; D005260:Female; D006801:Humans; D009362:Neoplasm Metastasis / Q000473:pathology*; D009376:Neoplasms, Hormone-Dependent / Q000097:blood / Q000188:drug therapy / Q000473:pathology; D009360:Neoplastic Cells, Circulating; D011446:Prospective Studies
chemical_list: D000970:Antineoplastic Agents; D018931:Antineoplastic Agents, Hormonal; D020847:Estrogen Receptor Modulators; D004967:Estrogens