Evidence for an association of TP53 codon 72 polymorphism with breast cancer risk.
BACKGROUND
A common Arg/Pro polymorphism at codon 72 of the TP53 gene has been investigated as a risk factor for cancer in different populations. So far, the results have been controversial. Our purpose was to investigate the association of this polymorphism with breast carcinoma in women from Southern Brazil, a high-risk area for breast cancer.


METHODS
Blood samples collected from 118 women with primary breast carcinoma and from 202 female blood donors were analyzed through polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing.


RESULTS
The relative frequency of each allele was 0.75 for Arg and 0.25 for Pro in patients with cancer, and 0.62 for Arg and 0.38 for Pro in normal controls (P < 0.001). The Arg/Arg genotype was significantly associated with an increased risk for breast cancer (OR 2.9; 95% CI 1.43-3.6; P < 0.002). No correlation between the genotype distribution and specific prognostic predictors for the disease outcome was observed.


CONCLUSIONS
TP53 codon 72 polymorphism might be implicated in breast carcinogenesis, with the Arg/Arg genotype being associated with an increased susceptibility for this malignancy.
Damin
Andrea P S
AP
Laboratory of Molecular Biology and Department of Pathology, Fundação Faculdade Federal de Ciências Médicas de Porto Alegre (FFFCMPA), Rua Sarmento Leite 245, Porto Alegre, RS 90050-170, Brazil. andreapsd@terra.com.br
Frazzon
Ana P G
AP
Damin
Daniel C
DC
Roehe
Adriana
A
Hermes
Vanessa
V
Zettler
Claudio
C
Alexandre
Claudio O P
CO
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001120:Arginine / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D001943:Breast Neoplasms / Q000453:epidemiology / Q000235:genetics*; D044584:Carcinoma, Ductal / Q000453:epidemiology / Q000235:genetics*; D018275:Carcinoma, Lobular / Q000453:epidemiology / Q000235:genetics*; D003062:Codon; D005260:Female; D016158:Genes, p53; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011392:Proline / Q000235:genetics; D012307:Risk Factors
D003062:Codon; D001120:Arginine; D011392:Proline