title: Identification of genes differentially regulated by transcription factor, AP-2delta.
abstract: In an effort to better understand the transcriptional program regulated by transcription factor, AP-2delta, we used cDNA microarray to evaluate the relative expression of human genes in AD293 cells by exogenous expression of AP-2delta. Microarray showed 29 genes that were up-regulated and 39 genes with a down-regulated expression pattern. Among the identified genes were those encoding transcription factors, signal transduction molecules, kinases, as well as genes regulating cell growth, differentiation, and cell adhesion, a set of genes suggested to be regulated by AP-2. The results of microarray was validated for some regulated genes by real-time PCR analysis. These observations shed novel insight into the mechanism of AP-2delta action and provide a range of target genes for further investigation.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Sun
authors.forename: Shengdong
authors.initials: S
authors.lastname: Huang
authors.forename: Qihan
authors.initials: Q
authors.lastname: Wu
authors.forename: Shaohua
authors.initials: S
authors.lastname: Gu
authors.forename: Xuping
authors.initials: X
authors.lastname: Fu
authors.forename: Ke
authors.initials: K
authors.lastname: Yu
authors.forename: Fanglin
authors.initials: F
authors.lastname: Lu
authors.forename: Chaoneng
authors.initials: C
authors.lastname: Ji
authors.forename: Congjing
authors.initials: C
authors.lastname: Feng
authors.forename: Ruping
authors.initials: R
authors.lastname: Sun
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
mesh_terms: D001665:Binding Sites; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050656:Transcription Factor AP-2 / Q000378:metabolism*
chemical_list: D050656:Transcription Factor AP-2