DNA elements modulating the KARS12 chromosomal replicator in Kluyveromyces lactis.
Eukaryotic chromosomal DNA replication is initiated by a highly conserved set of proteins that interact with cis-acting elements on chromosomes called replicators. Despite the conservation of replication initiation proteins, replicator sequences show little similarity from species to species in the small number of organisms that have been examined. Examination of replicators in other species is likely to reveal common features of replicators. We have examined a Kluyeromyces lactis replicator, KARS12, that functions as origin of DNA replication on plasmids and in the chromosome. It contains a 50-bp region with similarity to two other K. lactis replicators, KARS101 and the pKD1 replication origin. Replacement of the 50-bp sequence with an EcoRI site completely abrogated the ability of KARS12 to support plasmid and chromosomal DNA replication origin activity, demonstrating this sequence is a common feature of K. lactis replicators and is essential for function, possibly as the initiator protein binding site. Additional sequences up to 1 kb in length are required for efficient KARS12 function. Within these sequences are a binding site for a global regulator, Abf1p, and a region of bent DNA, both of which contribute to the activity of KARS12. These elements may facilitate protein binding, protein/protein interaction and/or nucleosome positioning as has been proposed for other eukaryotic origins of DNA replication.
Irene
Carmela
C
Dipartimento di Biologia Cellulare e dello Sviluppo, Università La Sapienza, Piazzale A. Moro, 5, Roma, Italy.
Maciariello
Clelia
C
Micheli
Gioacchino
G
Theis
James F
JF
Newlon
Carol S
CS
Fabiani
Lucia
L
D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015825:Chromosomes, Fungal / Q000235:genetics; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D007716:Kluyveromyces / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D018741:Replication Origin
D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins