MtHAP2-1 is a key transcriptional regulator of symbiotic nodule development regulated by microRNA169 in Medicago truncatula.
In the model legume Medicago truncatula, we identified a new transcription factor of the CCAAT-binding family, MtHAP2-1, for which RNA interference (RNAi) and in situ hybridization experiments indicate a key role during nodule development, possibly by controlling nodule meristem function. We could also show that MtHAP2-1 is regulated by microRNA169, whose overexpression leads to the same nodule developmental block as MtHAP2-1 RNAi constructs. The complementary expression pattern of miR169 and MtHAP2-1 and the phenotype of miR169-resistant MtHAP2-1 nodules strongly suggest, in addition, that the miR169-mediated restriction of MtHAP2-1 expression to the nodule meristematic zone is essential for the differentiation of nodule cells.
Combier
Jean-Philippe
JP
LIPM (Laboratoire des Interactions Plantes-Microorganismes) INRA-CNRS (Institut de National de la Recherche-Centre national de la recherche scientifique), 31326 Castanet-Tolosan, France.
Frugier
Florian
F
de Billy
Françoise
F
Boualem
Adnane
A
El-Yahyaoui
Fikri
F
Moreau
Sandra
S
Vernié
Tatiana
T
Ott
Thomas
T
Gamas
Pascal
P
Crespi
Martin
M
Niebel
Andreas
A
D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D046913:Medicago truncatula / Q000254:growth & development*; D018519:Meristem / Q000166:cytology / Q000648:ultrastructure; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012322:RNA Precursors / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053204:Root Nodules, Plant / Q000166:cytology / Q000254:growth & development*; D013559:Symbiosis; D014158:Transcription, Genetic
D035683:MicroRNAs; D010940:Plant Proteins; D012322:RNA Precursors; D012333:RNA, Messenger