Delivery after transfer of frozen-thawed embryos from in vitro-matured oocytes in a woman at risk for ovarian hyperstimulation syndrome.
The present report describes the birth of a healthy infant after cryopreservation of embryos produced from in vitro-matured oocytes retrieved from a woman at risk of developing ovarian hyperstimulation syndrome (OHSS) during conventional in vitro fertilization (IVF) cycles. A conventional long protocol including gonadotropin-releasing hormone agonist (GnRHa) and gonadotropins induced a risk of OHSS. Oocyte retrieval was performed on day 11 of the cycle, and 27 immature oocytes were obtained. Following incubation for 24 h in maturation medium, 74.1% (20/27) of the oocytes were at the metaphase II stage. Fourteen oocytes (14/20, 70.0%) were fertilized after intracytoplasmic sperm injection (ICSI) with her husband's spermatozoa and cultured for 3 days. On day 4 following oocyte retrieval, three embryos at the 8-16 cell stage were transferred into the woman's uterus, and five spare embryos were frozen. Since the fresh embryo transfer failed to result in pregnancy, three post-thaw embryos were transferred into the woman three months later. Transfer of the frozen embryos resulted in pregnancy with delivery of a healthy infant girl.
Chen
Juan
J
Jiangsu Province Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing, PR China.
Qian
Yun
Y
Feng
Ting
T
Cai
Ling-Bo
LB
Liu
Jia-Yin
JY
Zhang
Yan
Y
Ding
Wei
W
Mao
Yun-Dong
YD
D000328:Adult; D002478:Cells, Cultured; D015925:Cryopreservation; D036861:Delivery, Obstetric; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D006801:Humans; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D016471:Ovarian Hyperstimulation Syndrome / Q000209:etiology; D011247:Pregnancy; D012307:Risk Factors