Lactobacillus delbrueckii as a potential skin adjuvant for induction of type 1 immune responses.
We evaluated the ability of Lactobacillus delbrueckii UFV H2b20, a probiotic candidate, to stimulate the production of inflammatory cytokines and to induce macrophage activation and Th1 differentiation in peripheral blood mononuclear cells (PBMC) from healthy volunteers. Our results show that PBMC stimulated with heat-killed Lact. delbrueckii produced elevated levels of IL-12, IFN-gamma and TNF-alpha but no IL-10. IFN-gamma production was IL-12 dependent with NK cells as the main source. Furthermore, PBMC infected with Leishmania amazonensis presented elevated microbicidal activity when co-incubated with Lact. delbrueckii. Finally, Lact. delbrueckii was capable of inducing in vitro differentiation of L. amazonensis-specific Th1 cells. These findings suggest that this probiotic may be used as an adjuvant in vaccination protocols.
Castanheira
Laura Gomes
LG
Departamento de Ciências Biológicas, NUPEB, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brasil.
Castro
Juciane Maria de Andrade
JM
Martins-Filho
Olindo de Assis
OA
Nicoli
Jacques Robert
JR
Vieira
Leda Quercia
LQ
Afonso
Luis Carlos Crocco
LC
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000328:Adult; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D048668:Lactobacillus delbrueckii / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008262:Macrophage Activation; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D014611:Vaccination
D000276:Adjuvants, Immunologic; D016207:Cytokines