title: Effects of microbial inhibitors and modified atmosphere packaging on growth of Listeria monocytogenes and Salmonella enterica Typhimurium and on quality attributes of injected pork chops and sliced cured ham.
abstract: This study was designed to determine the inhibitory effects of modified atmosphere packaging (MAP), both alone and in combination with potassium lactate and sodium diacetate (PLSD), on selected pathogens common to pork products. Effects of the treatments on product quality also were assessed. The hypothesis was that high-CO2 MAP would increase the effectiveness of PLSD for inhibition of pork pathogens. Fresh chops from untreated pork loins and loins that were injected with PLSD were inoculated with Salmonella enterica Typhimurium, and slices of untreated hams and hams that were injected with PLSD were inoculated with Listeria monocytogenes. The samples were subjected to vacuum packaging (VP) or MAP with high concentrations (99.5 to 100%) of CO2. Pathogens were enumerated periodically during storage at 4 and 10 degrees C. Storage of pork chops at 4 degrees C slowed the growth of Salmonella Typhimurium, and there was little difference in pathogen numbers between VP and MAP samples. L. monocytogenes growth on ham slices at 4 degrees C was inhibited for up to 28 days by all of the treatments, but after 28 days, the VP-PLSD treatment had a greater inhibitory effect than did the other treatments. At 10 degrees C, the PLSD and MAP treatments each effectively inhibited the growth of the pathogens on pork chops and ham slices when compared with controls (VP). However, the results obtained with MAP plus PLSD at 10 degrees C were not different from those obtained with either MAP or PLSD alone. Therefore, the hypothesis was not supported; the high-CO2 atmosphere of the MAP did not increase the effectiveness of PLSD for inhibition of pathogens.
authors.affiliation: Department of Animal Science, 215 Meat Laboratory, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Michaelsen
authors.forename: Joseph G
authors.initials: JG
authors.lastname: Sebranek
authors.forename: James S
authors.initials: JS
authors.lastname: Dickson
mesh_terms: D000818:Animals; D002245:Carbon Dioxide; D015169:Colony Count, Microbial; D004357:Drug Synergism; D018857:Food Packaging / Q000379:methods*; D005520:Food Preservatives / Q000494:pharmacology*; D006801:Humans; D007773:Lactates / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000254:growth & development*; D008461:Meat Products / Q000382:microbiology / Q000592:standards; D010100:Oxygen / Q000378:metabolism; D017680:Potassium Compounds / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000254:growth & development*; D019346:Sodium Acetate / Q000494:pharmacology; D013552:Swine; D013696:Temperature; D013997:Time Factors; D014618:Vacuum
chemical_list: D005520:Food Preservatives; D007773:Lactates; D017680:Potassium Compounds; D002245:Carbon Dioxide; D019346:Sodium Acetate; D010100:Oxygen