Dynamic domains and geometrical properties of HIV-1 gp120 during conformational changes induced by CD4 binding.
The HIV-1 gp120 exterior envelope glycoprotein undergoes a series of conformational rearrangements while sequentially interacting with the receptor CD4 and coreceptor CCR5 or CXCR4 on the surface of host cells to initiate virus entry. Both the crystal structures of the HIV-1 gp120 core bound by the CD4 and antigen 17b, and the SIV gp120 core pre-bound by the CD4 are known. We have performed dynamic domain studies on the homology models of the CD4-bound and unliganded HIV-1 gp120 with modeled V3 and V4 loops to explore details of conformational changes, hinge axes, and hinge bending regions in the gp120 structures upon CD4 binding. Four dynamic domains were clustered and intricately motional modes for domain pairs were discovered. Together with the detailed comparative analyses of geometrical properties between the unliganded and liganded gp120 models, an induced fit model was proposed to explain events accompanying the CD4 engagement to the gp120, which provided new insight into the dynamics of the molecular induced binding mechanism that complements the molecular dynamics and crystallographic studies.
Liu
Shu-Qun
SQ
Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, 650091, People's Republic of China.
Liu
Shi-Xi
SX
Fu
Yun-Xin
YX
D001665:Binding Sites; D015704:CD4 Antigens / Q000737:chemistry / Q000378:metabolism*; D018360:Crystallography, X-Ray; D015699:HIV Envelope Protein gp120 / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D054398:Receptors, CCR4; D019718:Receptors, CXCR4 / Q000737:chemistry* / Q000378:metabolism; D019707:Receptors, Chemokine / Q000737:chemistry* / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism
C515354:CCR4 protein, human; D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D054398:Receptors, CCR4; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine; D014764:Viral Proteins