Chemiluminescent detection of DNA hybridization using gold nanoparticles as labels.
A chemiluminescent (CL) detection method has been developed for DNA hybridization. The assay relies on a sandwich-type DNA hybridization in which gold nanoparticles modified with alkylthiol-capped oligonucleotide strands are used as probes to monitor the presence of the specific target DNA. The AuCl(4)(-), which is the dissolving product of the gold nanoparticles anchored on the DNA hybrids, serves as an analyte in the H(2)O(2)-luminol- AuCl(4)(-) CL reaction for the indirect measurement of the target DNA. The combination of the remarkable sensitivity of the CL analysis with the large number of AuCl(4)(-) released from each DNA hybrid allows a detection limit at levels as low as 0.1 pM of the target DNA. Moreover, with a further silver amplification step, the detection limit will be pushed down to the femtomolar domain.
Li
Zheng-Ping
ZP
College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, Hebei, China. lzpbd@mail.hbu.edu.cn
Liu
Cheng-Hui
CH
Fan
Yong-Shan
YS
Duan
Xin-Rui
XR
D004247:DNA / Q000032:analysis*; D006046:Gold; D008163:Luminescent Measurements; D008165:Luminol; D053768:Metal Nanoparticles; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis; D012680:Sensitivity and Specificity
D015345:Oligonucleotide Probes; D008165:Luminol; D006046:Gold; D004247:DNA