title: Recovery of Salmonella using a combination of selective enrichment media and antimicrobial resistance of isolates in meat in Thailand.
abstract: From November 2004 to March 2005, 50 samples (chicken, pork and beef) of registered meat and non-registered meat were purchased from supermarkets and retail markets located in Bangkok, Thailand. Each sample was evaluated for Salmonella spp by a conventional method using combination of selective enrichment media (RV+MSRV) and compared with selective enrichment medium alone (DIASALM). Our study revealed the performance of RV+MSRV for the detection of Salmonella spp was significantly better than those of DIASALM alone since the recovery of Salmonella spp in both groups of meat was high using RV+MSRV, particularly in the registered meat. In addition, the recovery of serovars in registered meat was significantly higher than those in non-registered meat. Antimicrobial resistance of 62 Salmonella isolates in both groups of meat was determined for 10 antimicrobial drugs using the disk diffusion method. The results show that 100% of isolates from both groups were susceptible to amoxicillin/clavulanic acid, ciprofloxacin, cefotaxime and norfloxacin and 50-60% of isolates from both groups were resistant to tetracycline, streptomycin and ampicillin. Sixty percent of Salmonella isolates from meat showed multiresistance antimicrobial patterns.
authors.affiliation: WHO National Salmonella and Shigella Center, National Institute of Health, Department of Medical Science, Ministry of Public Health, Nonthaburi.
authors.forename: Aroon
authors.initials: A
authors.lastname: Bangtrakulnonth
authors.forename: Srirat
authors.initials: S
authors.lastname: Pornrungwong
authors.forename: Chaiwat
authors.initials: C
authors.lastname: Pulsrikarn
authors.forename: Sumalee
authors.initials: S
authors.lastname: Boonmar
authors.forename: Keiji
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000633:toxicity*; D002417:Cattle; D002645:Chickens; D003470:Culture Media; D052978:Disk Diffusion Antimicrobial Tests; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D008460:Meat / Q000382:microbiology*; D012475:Salmonella / Q000187:drug effects* / Q000302:isolation & purification*; D013552:Swine; D013785:Thailand
chemical_list: D000890:Anti-Infective Agents; D003470:Culture Media