title: [The interaction of acridine orange with anionic surfactant and its application to the determination of protein].
abstract: The change in the UV-absorption spectrum and fluorescence spectrum of acridine orange(AO) due to the addition of surfactant dodecyi benzene sulfonic acid sodium sait (SDBS) and bovine serum albumin(BSA) was studied. Meanwhile, the effects of the in situ formed AOAO dimer in SDBS as a fluorescence probe and BSA were discussed. A new method for the determination of BSA using fluorescence is presented. The results indicate that the method is sensitive and rapid. The linear range of determination is 0-4. 17 x 10(-7) mol x L(-1). The relative standard deviation is 1.9%, and the detection limit is 8.73 x 10(-10) mol x L(-1).
authors.affiliation: Key Laboratory of Food Science of MOE, Nanchang University, Nanchang 330047, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Wei-Wan
authors.initials: WW
authors.lastname: Zheng
authors.forename: Xiao-Xing
authors.initials: XX
authors.lastname: Wu
authors.forename: De-Hong
authors.initials: DH
authors.lastname: Zhou
mesh_terms: D000165:Acridine Orange / Q000737:chemistry*; D000818:Animals; D000838:Anions; D001557:Benzenesulfonates / Q000737:chemistry*; D002417:Cattle; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature
chemical_list: D000838:Anions; D001557:Benzenesulfonates; D005456:Fluorescent Dyes; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; C001114:dodecylbenzenesulfonic acid; D000165:Acridine Orange