title: GenomeRNAi: a database for cell-based RNAi phenotypes.
abstract: RNA interference (RNAi) has emerged as a powerful tool to generate loss-of-function phenotypes in a variety of organisms. Combined with the sequence information of almost completely annotated genomes, RNAi technologies have opened new avenues to conduct systematic genetic screens for every annotated gene in the genome. As increasing large datasets of RNAi-induced phenotypes become available, an important challenge remains the systematic integration and annotation of functional information. Genome-wide RNAi screens have been performed both in Caenorhabditis elegans and Drosophila for a variety of phenotypes and several RNAi libraries have become available to assess phenotypes for almost every gene in the genome. These screens were performed using different types of assays from visible phenotypes to focused transcriptional readouts and provide a rich data source for functional annotation across different species. The GenomeRNAi database provides access to published RNAi phenotypes obtained from cell-based screens and maps them to their genomic locus, including possible non-specific regions. The database also gives access to sequence information of RNAi probes used in various screens. It can be searched by phenotype, by gene, by RNAi probe or by sequence and is accessible at http://rnai.dkfz.de.
authors.affiliation: German Cancer Research Center, Boveri-Group Signaling and Functional Genomics, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Horn
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Arziman
authors.forename: Juerg
authors.initials: J
authors.lastname: Berger
authors.forename: Michael
authors.initials: M
authors.lastname: Boutros
mesh_terms: D000818:Animals; D030541:Databases, Genetic; D023281:Genomics; D020407:Internet; D015341:Nucleic Acid Probes / Q000737:chemistry; D010641:Phenotype; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry; D014584:User-Computer Interface
chemical_list: D015341:Nucleic Acid Probes; D034741:RNA, Small Interfering