Assembly of feline calicivirus-like particle and its immunogenicity.
Virus-like particles (VLPs) were produced in insect cells infected with a recombinant baculovirus containing the capsid gene of feline calicivirus strain F9 (FCV-F9). The FCV VLPs were morphologically and antigenically similar to the native virus and contained a single capsid protein with a molecular weight of approximately 60 kDa that reacted with FCV antiserum. Moreover, following immunization of rabbits, VLPs were able to elicit neutralizing antibodies against several FCV strains isolated from clinical samples. Our preliminary results showed that FCV-VLP could be considered a candidate vaccine against FCV infections.
Di Martino
Barbara
B
Department of Scienze Biomediche Comparate, University of Teramo, Piazza Aldo Moro 45, 64100 Teramo, Italy.
Marsilio
Fulvio
F
Roy
Polly
P
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D017250:Caliciviridae Infections / Q000276:immunology / Q000662:veterinary*; D017927:Calicivirus, Feline / Q000276:immunology* / Q000502:physiology*; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D002371:Cat Diseases / Q000276:immunology* / Q000821:virology*; D002415:Cats; D015967:Gene Expression Regulation, Viral; D007114:Immunization / Q000662:veterinary; D009500:Neutralization Tests; D011817:Rabbits; D019065:Virus Assembly / Q000502:physiology*
D000914:Antibodies, Viral; D036022:Capsid Proteins