title: Condensation of interphase chromosomes in nuclei of synchronized CHO cells.
abstract: The escape of individual interphase chromosomes from nuclei of reversibly permeabilized Chinese hamster ovary (CHO) cells was utilized for the visualization of condensing interphase chromosomes in a cell cycle-dependent manner in synchronized cells. Major interphase chromosomal forms include: (a) mid-S-phase globular chromosomes at 3.0 C-value, (b) late mid-S-phase fibrous hemicircular forms (3.3 C), (c) late-S-phase supercoiled ribbons (3.7 C), and (d) end-S-phase elongated, bent prechromosomal structures (4.0 C).
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen, Hungary. bgaspar@delfin.klte.hu
authors.forename: Gaspar
authors.initials: G
authors.lastname: Banfalvi
mesh_terms: D000818:Animals; D016466:CHO Cells; D002467:Cell Nucleus / Q000648:ultrastructure*; D002469:Cell Separation; D033481:Chromosomes, Mammalian / Q000378:metabolism* / Q000648:ultrastructure; D006224:Cricetinae; D005434:Flow Cytometry; D007399:Interphase; D008856:Microscopy, Fluorescence