title: Identification of non-specific alkaline phosphatases in hyphal cells of the fungus Neurospora crassa by in situ histochemistry.
abstract: The present study was designed to identify alkaline phosphatases in non-permeabilized hyphal cells of the fungus Neurospora crassa by staining these enzymatic activities with a modified azo dye coupling method. Our strategy allowed the identification of three non-specific alkaline phosphatase activities, one of them possibly being a novel putative enzyme, which is not responsive to either Mg(2+) or EDTA. Another alkaline phosphatase activity, whose location in the hyphal cell is regulated by phosphate, is stimulated by Mg(2+), inhibited by EDTA, and somehow dependent on the expression of the pho-2(+) -encoded Pi-repressible alkaline phosphatase.
authors.affiliation: Pró-Reitoria de Pesquisa, Pós-Graduação e Extensão, Universidade de Uberaba, Uberaba, MG, Brasil.
authors.forename: G
authors.initials: G
authors.lastname: Thedei
authors.forename: A
authors.initials: A
authors.lastname: Rossi
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000235:genetics; D002462:Cell Membrane; D004492:Edetic Acid; D006651:Histocytochemistry; D025301:Hyphae / Q000201:enzymology*; D009492:Neurospora crassa / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D004492:Edetic Acid; D000469:Alkaline Phosphatase