Synthesis of glyco(lipo)peptides by liposome-mediated native chemical ligation.
Although native chemical ligation (NCL) is emerging as a powerful method for the assembly of (glyco)peptide building blocks, its applicability is reduced when peptide segments are poorly soluble in aqueous buffer. We have found that incorporating reactants in liposomes allows NCL of lipophilic peptides and lipopeptides. Furthermore, the reaction rates of liposome-mediated NCL are higher than traditional reaction conditions resulting in improved yields. [reaction: see text]
Ingale
Sampat
S
Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
Buskas
Therese
T
Boons
Geert-Jan
GJ
D000595:Amino Acid Sequence; D002021:Buffers; D019496:Cancer Vaccines / Q000737:chemistry; D000939:Epitopes; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D007202:Indicators and Reagents; D008081:Liposomes / Q000737:chemistry*; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000737:chemistry; D012995:Solubility
D002021:Buffers; D019496:Cancer Vaccines; D000939:Epitopes; D006020:Glycopeptides; D007202:Indicators and Reagents; D008081:Liposomes; D018396:Mucin-1