[Transfection of dominant negative MyD88 decreases IL-8 production in bacteria-infected airway epithelial cells].
Interleukin-8 (IL-8) is an important activator and chemoattratant of neutrophils and has been implicated in airway inflammatory diseases. To explore the new gene therapeutic strategies for airway inflammation, plasmid expressing dominant negative myeloid differentiation protein (MyD88 DN) was constructed and transfected into human airway epithelial cell lines A549 and SPC-A-I. The cells were challenged with M. tuberculosis, P. aeruginosa or K. pneumoniae and the release of IL-8 was measured using ELISA. The results showed that the supernatants of M. tuberculosis and R. aeruginosa enhanced IL-8 release from the epithelial cells; and transfection of MyD88 DN diminished this effect. MyD88 DN also reduced IL-8 release from cells induced by live bacteria of P. aeruginosa or K. pneumoniae. These data suggest that MyD88 could be used as a target gene in the gene therapy of airway inflammation.
Feng
Yan
Y
Research Institute of Infection and Immunity of West China Medical Center, Sichuan University, Chengdu.
Wang
Fang
F
Chen
Xiangwen
X
Feng
Yun
Y
Huang
Ning
N
Wang
Boyao
B
Wu
Qi
Q
D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D009169:Mycobacterium tuberculosis; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics*; D011550:Pseudomonas aeruginosa; D014162:Transfection
D016209:Interleukin-8; C507779:MYD88 protein, human; D053594:Myeloid Differentiation Factor 88