[Characterization of the mRNA profile in ejaculated spermatozoa from healthy fertile men].
OBJECTIVE
To explore the complexity of mRNA in the ejaculated sperm from healthy fertile men.


METHODS
Semen samples were collected from 10 healthy fathers. The swim-up method was adopted to purify the sperm from possible contamination of somatic cells and the spermatozoal total RNA extracted by Trizol was used for SAGE library analysis.


RESULTS
A totle of 21 052 SAGE raw tags were sequenced from 877 clones and 2 712 unique tags that occurred at least twice in the library were given further analysis. 19.7% of the unique tags had no match in the existing SAGE map, representing novel genes. Molecular function analysis revealed 67% of unique tags related to protein binding or nucleic acid binding categories, 41% to catalytic activity, 13% to message transducer activity, and 10% to transporter, structural and transcription regulator activity, respectively.


CONCLUSIONS
There exists a complex repertoire of mRNAs in the ejaculated spermatozoa from fertile men.
Zhao
Yang-Xing
YX
School of Medicine, Shanghai Jiao-tong University, Shanghai 200240, China. yangxingzhao2000@yahoo.com
Li
Qiao-Li
QL
Wang
Zhao-Xia
ZX
Wang
Yi-Fei
YF
Wang
Lian-Yun
LY
Qiao
Zhong-Dong
ZD
D000328:Adult; D004542:Ejaculation; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D013094:Spermatozoa / Q000502:physiology*
D012333:RNA, Messenger