title: Quantitative analysis of herpes simplex virus type 1-specific memory B cells generated by different routes of infection.
abstract: We compared the herpes simplex virus type 1 (HSV)-specific memory B cell (MBC) populations generated by footpad and intranasal infection in mice. Both routes of infection generated transient antibody-secreting cell responses in the draining lymph nodes and spleen, and sustained circulating IgG. HSV-specific IgG MBCs, analyzed by limiting dilution assay approximately 8 weeks after infection, were distributed in a range of lymph nodes and in the spleen and Peyer's patches. Overall, the route of infection had little effect on the MBC frequency in each anatomical location. Interestingly, after both routes of infection there was a trend towards preferential MBC accumulation in the mediastinal lymph node. Intravaginal challenge of mice primed by footpad or intranasal infection generated similar secondary IgG responses. Our findings indicate that the widespread dispersion of MBCs to lymphoid tissues throughout the body is largely independent of the route of infection, but may be influenced by tissue-specific factors.
authors.affiliation: Department of Microbiology, M409 Walters Life Sciences Bldg., 1414 W. Cumberland Ave, University of Tennessee, Knoxville, TN 37996, USA. dvanitha@utk.edu
authors.forename: Daisy J
authors.initials: DJ
authors.lastname: Vanitha
authors.forename: Hye Mee
authors.initials: HM
authors.lastname: Joo
authors.forename: Barry T
authors.initials: BT
authors.lastname: Rouse
authors.forename: Mark Y
authors.initials: MY
authors.lastname: Sangster
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D006561:Herpes Simplex / Q000097:blood / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D007114:Immunization; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007156:Immunologic Memory; D008198:Lymph Nodes / Q000276:immunology; D018655:Lymphocyte Count; D008482:Mediastinum; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D010581:Peyer's Patches / Q000276:immunology; D013154:Spleen / Q000276:immunology
chemical_list: D007074:Immunoglobulin G