title: Synchronization and superovulation of mature cycling gilts for the collection of pronuclear stage embryos.
abstract: An efficient protocol was developed to synchronize and superovulate mature pigs for the collection of pronuclear stage embryos suitable for DNA microinjection. A timed and coordinated regimen of Lutalyse, PG600 and Chorulon along with daily checking for estrus allowed synchronization of groups of gilts having estrous cycles at regular intervals. Pigs 10-16 days after the beginning of standing estrus have been successfully synchronized into estrus using this protocol. A standard dose of each drug was used independent of size or age of the animal. One protocol averaged 38.9 ovulations and 31.1 one-cell embryos recovered per animal.
authors.affiliation: Department of Animal Science, North Carolina State University, Campus Box 7621, Raleigh, NC 27695-7621, USA.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Sommer
authors.forename: E Bruce
authors.initials: EB
authors.lastname: Collins
authors.forename: Jose L
authors.initials: JL
authors.lastname: Estrada
authors.forename: Robert M
authors.initials: RM
authors.lastname: Petters
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D015237:Dinoprost / Q000031:analogs & derivatives / Q000494:pharmacology; D004622:Embryo, Mammalian; D030762:Estrous Cycle; D004973:Estrus Synchronization / Q000379:methods*; D005260:Female; D013480:Superovulation / Q000187:drug effects / Q000502:physiology*; D013552:Swine / Q000502:physiology*
chemical_list: D006063:Chorionic Gonadotropin; D015237:Dinoprost; C010714:dinoprost tromethamine