title: Cytogenetic analysis of B-cell posttransplant lymphoproliferations validates the World Health Organization classification and suggests inclusion of florid follicular hyperplasia as a precursor lesion.
abstract: Cytogenetic abnormalities in B-cell posttransplant lymphoproliferative disorders (PTLD) have not been well characterized. We thus performed cytogenetic analysis of 28 cases of B-cell PTLD, 1 infectious mononucleosis (IM)-like lesion, 9 polymorphic PTLD, 17 monomorphic PTLD, and 1 classical Hodgkin lymphoma (HL), and correlated the karyotypic findings with the phenotype, Epstein-Barr virus infection status, and clinical outcome. Karyotypes of 19 cases of posttransplant florid follicular hyperplasia (FFH) were also analyzed. Informative karyotypes were obtained in 20 (71.4%) of 28 PTLDs and 18 (94.7%) of 19 FFHs. Clonal karyotypic abnormalities were detected in 13 (65%) of 20 PTLDs, including 9 (75%) of 12 monomorphic PTLDs, 2 (33.3%) of 6 polymorphic PTLDs, 1 IM-like lesion, and 1 HL, and 2 (11.1%) of 18 FFHs. Recurrent chromosome breaks at 1q11-21 (n = 6, including 1 FFH), 14q32 (n = 3, including 1 FFH), 16p13 (n = 3), 11q23-24 (n = 2), and 8q24 (c-MYC) (n = 2); gains of chromosome 7 (n = 4), X (n = 3), 2 (n = 3), 12 (n = 2); and loss of chromosome 22 (n = 2, including 1 IM-like lesion) were identified. The presence of cytogenetic abnormalities did not correlate with PTLD phenotype, Epstein-Barr virus infection, or clinical outcome. We describe novel karyotypic aberrations in PTLD and report clonal cytogenetic abnormalities in posttransplant FFH and an IM-like lesion for the first time. Our findings provide validation of the current World Health Organization classification of PTLD and also suggest incorporation of FFH as the earliest recognizable precursor of PTLD.
authors.affiliation: Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Efsevia
authors.initials: E
authors.lastname: Vakiani
authors.forename: Subhadra V
authors.initials: SV
authors.lastname: Nandula
authors.forename: Shivakumar
authors.initials: S
authors.lastname: Subramaniyam
authors.forename: Christian E
authors.initials: CE
authors.lastname: Keller
authors.forename: Bachir
authors.initials: B
authors.lastname: Alobeid
authors.forename: Vundavalli V
authors.initials: VV
authors.lastname: Murty
authors.forename: Govind
authors.initials: G
authors.lastname: Bhagat
mesh_terms: D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002999:Clone Cells / Q000378:metabolism / Q000473:pathology; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000821:virology; D005260:Female; D005838:Genotype; D006801:Humans; D006965:Hyperplasia; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D016031:Liver Transplantation / Q000009:adverse effects; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology*; D008232:Lymphoproliferative Disorders / Q000145:classification / Q000209:etiology* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D016377:Organ Transplantation / Q000009:adverse effects; D010641:Phenotype; D014944:World Health Organization