Prevalence of human cytomegalovirus UL97 D605E mutation in transplant recipients in China.
Human cytomegalovirus (CMV) resistance to gancyclovir (GCV) occurs via mutation in the UL97 gene, ethylene diamine tetraacetic acid blood samples were obtained from 23 transplant recipients who received a GCV implant. A nested polymerase chain reaction amplifying UL97 codons 450 to 672 was performed. Nested amplifications were sequenced directly. No known UL97 GCV resistance mutations were found. Eighteen of 23 patients (78%) had revealed mutations at codon 605 (D to E). Mutant D605E may reverse to wild-type during the follow-up treatment. We conclude that human CMV UL97 D605E mutation occurred in Chinese transplant recipients. This mutation may be regarded as a natural sequence variant.
Zhou
L
L
The First Affiliated Hospital of Zhejiang University School of Medicine, Key Lab of Combined Multi-organ Transplantation, Hangzhou, Zhejiang, China. linzhou8@mail.hz.zj.cn
Fan
J
J
Zheng
S S
SS
Ma
W H
WH
D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D002681:China; D003062:Codon; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000453:epidemiology / Q000821:virology; D017931:DNA Primers; D005784:Gene Amplification; D014644:Genetic Variation; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D009154:Mutation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D011183:Postoperative Complications / Q000453:epidemiology / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003062:Codon; D017931:DNA Primers; D017853:Phosphotransferases (Alcohol Group Acceptor); C075365:ganciclovir kinase