title: Structural stabilization and functional improvement of horseradish peroxidase upon modification of accessible lysines: experiments and simulation.
abstract: Horseradish peroxidase (HRP) is an important heme enzyme with enormous medical diagnostic, biosensing, and biotechnological applications. Thus, any improvement in the applicability and stability of the enzyme is potentially interesting. We previously reported that covalent attachment of an electron relay (anthraquinone 2-carboxylic acid) to the surface-exposed Lys residues successfully improves electron transfer properties of HRP. Here we investigated structural and functional consequences of this modification, which alters three accessible charged lysines (Lys-174, Lys-232, and Lys-241) to the hydrophobic anthraquinolysine residues. Thermal denaturation and thermoinactivation studies demonstrated that this kind of modification enhances the conformational and operational stability of HRP. The melting temperature increased 3 degrees C and the catalytic efficiency enhanced by 80%. Fluorescence and circular dichroism investigations suggest that the modified HRP benefits from enhanced aromatic packing and more buried hydrophobic patches as compared to the native one. Molecular dynamics simulations showed that modification improves the accessibility of His-42 and the heme prosthetic group to the peroxide and aromatic substrates, respectively. Additionally, the hydrophobic patch, which functions as a binding site or trap for reducing aromatic substrates, is more extended in the modified enzyme. In summary, this modification produces a new derivative of HRP with enhanced electron transfer properties, catalytic efficiency, and stability for biotechnological applications.
authors.affiliation: Laboratory of Microanalysis, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Navid
authors.initials: N
authors.lastname: Mogharrab
authors.forename: Hedayatollah
authors.initials: H
authors.lastname: Ghourchian
authors.forename: Mehriar
authors.initials: M
authors.lastname: Amininasab
mesh_terms: D001665:Binding Sites; D002384:Catalysis; D002942:Circular Dichroism; D003198:Computer Simulation; D004795:Enzyme Stability; D006735:Horseradish Peroxidase / Q000737:chemistry*; D007700:Kinetics; D008239:Lysine / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D006735:Horseradish Peroxidase; D008239:Lysine