First evidence of higher female recombination in a species with temperature-dependent sex determination: the saltwater crocodile.
The first evidence of genetic linkage and sex-specific recombination in the order Crocodylia is reported. This study was conducted using a resource pedigree of saltwater crocodiles consisting of 16 known-breeding pairs (32 adults) and 101 juveniles. A total of 21 microsatellite loci were available for analysis. Ten of the 21 loci showed linkage with 4 linkage groups: 3 pairwise (Cj131/Cj127, CUD68/Cj101, and Cj107/Cp10) and 1 four-locus (Cj122, CUD78, Cj16, and Cj104) being found. Linkage analysis on the 21 loci revealed evidence of sex-specific differences in recombination rates. All 5 nonzero interlocus intervals were longer in females than in males, with the 4-loci linkage group 3-fold longer in females than in males (41.63 cM and 14.1 cM, respectively). This is the first report of sex-specific recombination rates in a species that exhibits temperature-dependent sex determination.
Isberg
Sally Robyn
SR
Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Science, University of Sydney, NSW, Australia.
Johnston
Scott Maxwell
SM
Chen
Yizhou
Y
Moran
Christopher
C
D000489:Alligators and Crocodiles / Q000235:genetics*; D000818:Animals; D005260:Female; D008040:Genetic Linkage; D008297:Male; D018895:Microsatellite Repeats; D011995:Recombination, Genetic; D019849:Sex Determination Processes; D013045:Species Specificity; D013696:Temperature