Direct vaccination with pseudotype baculovirus expressing murine telomerase induces anti-tumor immunity comparable with RNA-electroporated dendritic cells in a murine glioma model.
Baculovirus pseudotyped with vesicular stomatitis virus G protein (Bac-VSV-G) was found to efficiently transduce and express transgenes on mammalian cells. In this study, this recombinant virus was used for induction of anti-tumor immunity against murine telomerase reverse transcriptase (mTERT) and was compared with RNA-electroporated dendritic cells (DCs) in a murine glioma model. Splenocytes from the mice vaccinated with Bac-VSV-G expressing mTERT (Bac-VSVG-mTERT) showed significantly increased numbers of mTERT-specific IFN-gamma-secreting T cells using an ELISPOT technique, and also showed increased NK cell activity. In addition, the TERT-specific T cells activated by Bac-VSVG-mTERT and mTERT RNA-electroporated DCs were predominantly CD4+ T cells and CD8+ T cells, respectively. The protective anti-tumor effect of Bac-VSVG-mTERT was similar to that of mTERT RNA-electroporated DCs. These results suggest that the pseudotype baculovirus expressing TERT may be a good candidate for a genetic vaccine for use in the treatment of malignant gliomas.
Kim
Chang-Hyun
CH
Catholic Research Institute of Medical Science, Catholic University of Korea, 505 Banpo-Dong, Seocho-Gu, Seoul 137-701, Republic of Korea.
Yoon
Jong-Sub
JS
Sohn
Hyun-Jung
HJ
Kim
Chung-Kwon
CK
Paik
Soon-Young
SY
Hong
Yong-Kil
YK
Kim
Tai-Gyu
TG
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D001932:Brain Neoplasms / Q000276:immunology*; D002460:Cell Line; D017931:DNA Primers; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D018274:Electroporation; D005260:Female; D005910:Glioma / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018411:Spodoptera; D019098:Telomerase / Q000008:administration & dosage* / Q000235:genetics
D017931:DNA Primers; D019098:Telomerase