Polyclonal antibodies mediated immobilization of a peroxidase from ammonium sulphate fractionated bitter gourd (Momordica charantia) proteins.
Polyclonal antibody bound Sepharose 4B support has been exploited for the immobilization of bitter gourd peroxidase directly from ammonium sulphate precipitated proteins. Immunoaffinity immobilized bitter gourd peroxidase exhibited high yield of immobilization. IgG-Sepharose 4B bound bitter gourd peroxidase showed a higher stability against heat, chaotropic agents (urea and guanidinium chloride), detergents (cetyl trimethyl ammonium bromide and Surf Excel), proteolytic enzyme (trypsin) and water-miscible organic solvents (propanol, THF and dioxane). The activity of immobilized bitter gourd peroxidase was significantly enhanced in the presence of cetyl trimethyl ammonium bromide and after treatment with trypsin as compared to soluble enzyme.
Fatima
Aiman
A
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, UP, India.
Husain
Qayyum
Q
D000645:Ammonium Sulfate / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D005591:Chemical Fractionation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D029777:Momordica charantia / Q000378:metabolism*; D009195:Peroxidase / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D011485:Protein Binding
D000911:Antibodies, Monoclonal; D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D010940:Plant Proteins; D009195:Peroxidase; D000645:Ammonium Sulfate