title: Dialysis-related genotoxicity: sister chromatid exchanges and DNA lesions in T and B lymphocytes of uremic patients. Genomic damage in patients on hemodiafiltration.
abstract: OBJECTIVE
  Patients with chronic renal failure show the presence of massive oxidative genome damage but the role played by dialysis is still a controversial issue. The aim of our study was to verify the genomic damage in B- and T-lymphocyte subpopulations of uremic patients after a single hemodiafiltration session.
  
  
  METHODS
  We enrolled 30 patients on maintenance acetate-free biofiltration and 25 age-matched healthy volunteers and studied chromosomal alterations.
  
  
  RESULTS
  Our data show that the basal levels of DNA damage, the number of sister chromatid exchanges and basal high-frequency cells levels are significantly higher in patients on hemodiafiltration than in controls and in T lymphocytes than in B cells.
  
  
  CONCLUSIONS
  These findings suggest that hemodialytic treatment could represent a potential source of damage, maybe through the oxidative action of the extracorporeal circuit components, which might explain the well-known T-specific immunodeficiency correlated with uremia.
authors.affiliation: Chair of Nephrology, Department of Internal Medicine, University of Messina, Messina, Italy. buemim@unime.it
authors.forename: M
authors.initials: M
authors.lastname: Buemi
authors.forename: F
authors.initials: F
authors.lastname: Floccari
authors.forename: C
authors.initials: C
authors.lastname: Costa
authors.forename: C
authors.initials: C
authors.lastname: Caccamo
authors.forename: N
authors.initials: N
authors.lastname: Belghity
authors.forename: S
authors.initials: S
authors.lastname: Campo
authors.forename: F
authors.initials: F
authors.lastname: Pernice
authors.forename: G
authors.initials: G
authors.lastname: Bonvissuto
authors.forename: G
authors.initials: G
authors.lastname: Coppolino
authors.forename: A
authors.initials: A
authors.lastname: Barillà
authors.forename: M
authors.initials: M
authors.lastname: Criseo
authors.forename: E
authors.initials: E
authors.lastname: Crascì
authors.forename: L
authors.initials: L
authors.lastname: Nostro
authors.forename: A
authors.initials: A
authors.lastname: Arena
mesh_terms: D001402:B-Lymphocytes / Q000473:pathology; D004249:DNA Damage; D005260:Female; D017583:Hemodiafiltration / Q000009:adverse effects*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000150:complications* / Q000473:pathology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D012854:Sister Chromatid Exchange; D013601:T-Lymphocytes / Q000473:pathology; D014511:Uremia / Q000150:complications / Q000473:pathology / Q000628:therapy