Iron- and 4-hydroxy-2-alkylquinoline-containing periplasmic inclusion bodies of Pseudomonas aeruginosa: a chemical analysis.
Dark aggregated particles were seen on pellets of iron-rich, mid-logarithmic phase Pseudomonas aeruginosa. Transmission electron microscopy of these cells showed inclusion bodies in periplasmic vacuoles. Aggregated particles isolated from the spent medium of these cells contained iron as indicated by atomic absorption spectroscopy and by electron paramagnetic resonance spectroscopy that revealed Fe(3+). Scanning electron microscopy/energy dispersive X-ray analysis of whole cells revealed the presence of iron-containing particles beneath the surface of the cell, indicating that the isolated aggregates were the intracellular inclusion bodies. Collectively, mass spectroscopy and nuclear magnetic resonance spectroscopy of the isolated inclusion bodies revealed the presence of 3,4-dihydroxy-2-heptylquinoline which is the Pseudomonas quinolone signaling compound (PQS) and an iron chelator; 4-hydroxy-2-heptylquinoline (pseudan VII), which is an iron chelator, antibacterial compound and precursor of PQS; 4-hydroxy-2-nonylquinoline (pseudan IX) which is an iron chelator and antibacterial compound; 4-hydroxy-2-methylquinoline (pseudan I), and 4-hydroxy-2-nonylquinoline N-oxide.
Royt
Paulette W
PW
Molecular and Microbiology Department, George Mason University, Fairfax, VA 22030, USA. proyt@gmu.edu
Honeychuck
Robert V
RV
Pant
Ramesh R
RR
Rogers
Magnus L
ML
Asher
Ludmila V
LV
Lloyd
John R
JR
Carlos
W E
WE
Belkin
Harvey E
HE
Patwardhan
Swati
S
D002855:Chromatography, Thin Layer; D004578:Electron Spin Resonance Spectroscopy; D008401:Gas Chromatography-Mass Spectrometry; D006912:Hydroxyquinolines / Q000737:chemistry*; D002479:Inclusion Bodies / Q000737:chemistry* / Q000648:ultrastructure; D007501:Iron / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000254:growth & development / Q000648:ultrastructure; D021241:Spectrometry, Mass, Electrospray Ionization
D006912:Hydroxyquinolines; D007501:Iron