Not all cytokine-producing CD8+ T cells suppress simian immunodeficiency virus replication.
Current assays of CD8+ T-lymphocyte function measure cytokine production rather than the ability of these lymphocytes to suppress viral replication. Here we show that CD8+ T-cell clones recognizing the same epitope vary enormously in the ability to suppress simian immunodeficiency virus SIVmac239 replication in an in vitro suppression assay. However, all Nef(165-173)IW9- and Vif(66-73)HW8-specific clones from elite controllers effectively suppressed SIV replication. Interestingly, in vitro suppression efficacy was not always associated with the ability to produce gamma interferon, tumor necrosis factor alpha, or interleukin-2.
Chung
Chungwon
C
Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715-1299, USA.
Lee
Wonhee
W
Loffredo
John T
JT
Burwitz
Benjamin
B
Friedrich
Thomas C
TC
Giraldo Vela
Juan Pablo
JP
Napoe
Gnankang
G
Rakasz
Eva G
EG
Wilson
Nancy A
NA
Allison
David B
DB
Watkins
David I
DI
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis*; D008253:Macaca mulatta; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000502:physiology; D019562:Viral Load; D014779:Virus Replication / Q000276:immunology* / Q000502:physiology
D016207:Cytokines