title: Hybrid composites of calcium phosphate granules, fibrin glue, and bone marrow for skeletal repair.
abstract: Synthetic bone substitutes, such as calcium phosphate ceramics, give good results in clinical applications. In order to adapt to surgical sites, bioceramics come in the form of blocks or granules, and are either dense or porous. Combining these bioceramics with fibrin glue provides a mouldable and self-hardening composite biomaterial with the biochemical properties of each component. Critical-sized defects in the femoral condyle of rabbits were filled with TricOs/fibrin glue/bone marrow hybrid/composite material. The TricOs granules (1-2 mm) were composed of hydroxyapatite and beta tricalcium phosphate (60/40 in weight). The fibrin glue was composed of fibrinogen, thrombin and other biological factors and mixed with MBCP granules either simultaneously or sequentially. Bone marrow was also added to the MBCP/fibrin composite prior to filling the defects. After 3, 6, 12, and 24 weeks of implantation, the newly-formed bone was analysed with histology, histomorphometry and mechanical tests. The newly-formed bone had grown centripetally. Simultaneous application of fibrin glue showed better results for mechanical properties than sequential application after 6 weeks. Around 40% of bone had formed after 24 weeks in the three groups. Although the addition of bone marrow did not improve bone formation, the MBCP/fibrin material could be used in clinical bone filling applications.
authors.affiliation: Inserm, U791, Laboratoire d'Ingénierie Ostéo-Articulaire et Dentaire, LIOAD, Nantes, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Le Nihouannen
authors.forename: Eric
authors.initials: E
authors.lastname: Goyenvalle
authors.forename: Eric
authors.initials: E
authors.lastname: Aguado
authors.forename: Paul
authors.initials: P
authors.lastname: Pilet
authors.forename: Melitta
authors.initials: M
authors.lastname: Bilban
authors.forename: Guy
authors.initials: G
authors.lastname: Daculsi
authors.forename: Pierre
authors.initials: P
authors.lastname: Layrolle
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001853:Bone Marrow / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry; D005260:Female; D005269:Femur / Q000473:pathology / Q000601:surgery; D015718:Fibrin Tissue Adhesive / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration; D010316:Particle Size; D011817:Rabbits; D013499:Surface Properties
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D015718:Fibrin Tissue Adhesive; C020243:calcium phosphate