title: Fabrication of PDMS/glass microchips by twofold replication of PDMS and its application in genetic analysis.
abstract: In this paper, we describe a simple method for fabrication of high quality poly(dimethylsiloxane) (PDMS)/glass microchip by twofold replica molding of PDMS. This technique first served to transfer the negative microchannels from the glass template to the PDMS substrate as a master, and then this PDMS master with positive microchannels was used to replicate the PDMS replica with negative microchannels. Finally, the PDMS replica was bound to a glass sheet by UV radiation. The fabricated microchips were successfully applied for the detection of C677T mutation from the human methylenetetrahydrofolate reductase gene.
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai Jiaotong University, Shanghai, PR China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Qu
authors.forename: Fuming
authors.initials: F
authors.lastname: Sang
authors.forename: Jicun
authors.initials: J
authors.lastname: Ren
mesh_terms: D001483:Base Sequence; D004252:DNA Mutational Analysis / Q000295:instrumentation / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D004129:Dimethylpolysiloxanes / Q000138:chemical synthesis*; D004586:Electrophoresis / Q000379:methods; D004867:Equipment Design; D005898:Glass; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics; D008855:Microscopy, Electron, Scanning; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D017354:Point Mutation; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004129:Dimethylpolysiloxanes; D042965:Methylenetetrahydrofolate Reductase (NADPH2)