Quantitative proteomic analysis of the budding yeast cell cycle using acid-cleavable isotope-coded affinity tag reagents.
Quantitative profiling of proteins, the direct effectors of nearly all biological functions, will undoubtedly complement technologies for the measurement of mRNA. Systematic proteomic measurement of the cell cycle is now possible by using stable isotopic labeling with isotope-coded affinity tag reagents and software tools for high-throughput analysis of LC-MS/MS data. We provide here the first such study achieving quantitative, global proteomic measurement of a time-course gene expression experiment in a model eukaryote, the budding yeast Saccharomyces cerevisiae, during the cell cycle. We sampled 48% of all predicted ORFs, and provide the data, including identifications, quantitations, and statistical measures of certainty, to the community in a sortable matrix. We do not detect significant concordance in the dynamics of the system over the time-course tested between our proteomic measurements and microarray measures collected from similarly treated yeast cultures. Our proteomic dataset therefore provides a necessary and complementary measure of eukaryotic gene expression, establishes a rich database for the functional analysis of S. cerevisiae proteins, and will enable further development of technologies for global proteomic analysis of higher eukaryotes.
Flory
Mark R
MR
Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459, USA. mflory@wesleyan.edu
Lee
Hookeun
H
Bonneau
Richard
R
Mallick
Parag
P
Serikawa
Kyle
K
Morris
David R
DR
Aebersold
Ruedi
R
D002247:Carbon Isotopes; D002453:Cell Cycle / Q000502:physiology*; D005800:Genes, Fungal / Q000235:genetics; D007553:Isotope Labeling / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry
D002247:Carbon Isotopes; D029701:Saccharomyces cerevisiae Proteins