title: High molecular weight adiponectin reduces apolipoprotein B and E release in human hepatocytes.
abstract: Low circulating levels of high molecular weight adiponectin (HMW-Apm) have been linked to dyslipidaemia and systemic HMW-Apm negatively correlates with very low density lipoprotein (VLDL), apolipoprotein B (ApoB), and ApoE and is positively associated with ApoA-I. Therefore, it was investigated whether HMW-Apm alters the hepatic synthesis of ApoB, ApoE, and ApoA-I or the activity of the hepatic ATP-binding cassette transporter A1 (ABCA1), as the main determinant of plasma HDL. HMW-Apm reduces hepatic ApoB and ApoE release whereas ABCA1 protein, activity and ApoA-I were not altered. Global gene expression analysis revealed that hepatic nuclear factor 4-alpha (HNF4-alpha) and HNF4-alpha regulated genes like ApoB are downregulated by HMW-Apm and this was confirmed at the mRNA and protein level. Therefore it is concluded that HMW-adiponectin may ameliorate dyslipidaemia by reducing the hepatic release of ApoB and ApoE, whereas ABCA1 function and ApoA-I secretion are not influenced.
authors.affiliation: Department of Internal Medicine I, Regensburg University Medical Center, D-93042 Regensburg, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Neumeier
authors.forename: Alexander
authors.initials: A
authors.lastname: Sigruener
authors.forename: Elke
authors.initials: E
authors.lastname: Eggenhofer
authors.forename: Johanna
authors.initials: J
authors.lastname: Weigert
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Weiss
authors.forename: Andreas
authors.initials: A
authors.lastname: Schaeffler
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Schlitt
authors.forename: Charalampos
authors.initials: C
authors.lastname: Aslanidis
authors.forename: Pompiliu
authors.initials: P
authors.lastname: Piso
authors.forename: Thomas
authors.initials: T
authors.lastname: Langmann
authors.forename: Gerd
authors.initials: G
authors.lastname: Schmitz
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schölmerich
authors.forename: Christa
authors.initials: C
authors.lastname: Buechler
mesh_terms: D052242:Adiponectin / Q000008:administration & dosage / Q000737:chemistry; D001055:Apolipoproteins B / Q000378:metabolism*; D001057:Apolipoproteins E / Q000378:metabolism*; D002478:Cells, Cultured; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D001055:Apolipoproteins B; D001057:Apolipoproteins E