The collapsin response mediator protein 1 (CRMP-1) and the promyelocytic leukemia zinc finger protein (PLZF) bind to UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE), the key enzyme of sialic acid biosynthesis.
Sialic acids (Sia) are expressed as terminal sugars in many glycoconjugates. They are involved in a variety of cell-cell interactions and therefore play an important role during development and regeneration. UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) is the key enzyme in the de novo synthesis of Sia and it is a regulator of cell surface sialylation. Inactivation of GNE in mice results in early embryonic lethality. Mutations in the GNE gene are of clinical relevance in hereditary inclusion body myopathy, but these mutations do not necessarily decrease the enzymatic activity of GNE. In this study, we searched for novel function of the GNE protein beside its enzymatic function in the Sia biosynthesis. We here report the identification of novel GNE-interacting proteins. Using a human prey matrix we identified four proteins interacting with GNE in a yeast two-hybrid assay. For two of them, the collapsin response mediator protein 1 and the promyelocytic leukemia zinc finger protein, we could verify protein-protein interaction with GNE.
Weidemann
Wenke
W
Institut für Biochemie und Molekularbiologie, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Arnimallee 22, 14195 Berlin-Dahlem, Germany.
Stelzl
Ulrich
U
Lisewski
Ulrike
U
Bork
Kaya
K
Wanker
Erich E
EE
Hinderlich
Stephan
S
Horstkorte
Rüdiger
R
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002238:Carbohydrate Epimerases / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D018922:HL-60 Cells; D006639:Histidine; D006801:Humans; D047468:Immunoprecipitation; D051741:Kruppel-Like Transcription Factors; D019158:N-Acetylneuraminic Acid / Q000096:biosynthesis*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009842:Oligopeptides; D016716:PC12 Cells; D010750:Phosphoproteins / Q000378:metabolism*; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D011485:Protein Binding; D051381:Rats; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D000098855:Collapsin Response Mediator Protein 1
D000906:Antibodies; D004268:DNA-Binding Proteins; C471213:His-His-His-His-His-His; D051741:Kruppel-Like Transcription Factors; D009419:Nerve Tissue Proteins; D009842:Oligopeptides; D010750:Phosphoproteins; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D014157:Transcription Factors; D000098855:Collapsin Response Mediator Protein 1; C092037:ZBTB16 protein, human; D006639:Histidine; D002238:Carbohydrate Epimerases; C100033:UDP acetylglucosamine-2-epimerase; D019158:N-Acetylneuraminic Acid