[Background study of HIV-1 drug resistant mutations in treatment-naive patients in liaoning province].
OBJECTIVE
To collect background information on drug resistance mutations in treatment-naïve HIV-1 infected individuals in Liaoning Province.


METHODS
Samples from 91 antiretroviral therapy-naïve patients were collected. The entire protease gene and 1-290 amino acids of the reverse transcriptase gene were amplified by nested PCR from provirus DNA and sequenced. The results were analyzed with HIVdb-Drug Resistance Algorithm, and genotypic resistance mutations were determined to particular anti-HIV drugs.


RESULTS
Totally 91 sequences were obtained, 3 of which displayed M46I mutations in the protease gene. Minor resistance mutation rate to protease inhibitors was 100%, including types of L63P (60.4%), V77I (60.4%), M36I/V (31.9%), A71V/T (22.0%), L10I (8.8%), and K20R (6.6%). Only one sequence carried reverse transcriptase related resistance mutations M184I.


CONCLUSIONS
About 4.4% of HIV-1 infected individuals in Liaoning Province carried strains with drug resistance mutations. Most treatment-naïve HIV-1 infected individuals in Liaoning Province were sensitive to the currently available antiviral medicines, but antiviral treatment must be in accordance with the strict procedures to keep better adherence and avoid the prevalence of drug-resistant strains.
Han
Xiao-xu
XX
Key Laboratory of AIDS Immunology of Ministry of Health, the First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Zhang
Min
M
Dai
Di
D
Lu
Chunming
C
Zhang
Zi-ning
ZN
Liu
Jing
J
Wang
Ya-nan
YN
Jiang
Yong-jun
YJ
Shang
Hong
H
D000328:Adult; D002681:China / Q000453:epidemiology; D024882:Drug Resistance, Viral / Q000235:genetics*; D005260:Female; D015658:HIV Infections / Q000188:drug therapy / Q000453:epidemiology*; D016333:HIV Protease / Q000235:genetics*; D054303:HIV Reverse Transcriptase / Q000235:genetics; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D009154:Mutation; D017422:Sequence Analysis, DNA
D054303:HIV Reverse Transcriptase; D016333:HIV Protease