title: Simple multichannel system for the measurement of the net water flux across biological tissues.
abstract: This paper describes the development of a simple system for measurement of net water movement through biological membrane barriers. The system is based on the detection of a water meniscus inside a polyethylene tube, which reflects the water movement inside one hemichamber of a modified Ussing chamber containing a membrane barrier. The detection device consists of a commercial computer-controlled flat bed scanner and specifically developed software. This system allows one to perform a relatively high number of individual experiments per physical unit. It is a flexible and affordable device, which allows comparatively more information per unit to be obtained than previously described methods.
authors.affiliation: California Animal Health and Food Safety Laboratory, San Bernardino Branch, University of California-Davis, 105 W Central Avenue, San Bernardino, CA 92408, USA. mmiyakaw@cahfs.ucdavis.edu
authors.forename: Mariano E
authors.initials: ME
authors.lastname: Fernández-Miyakawa
authors.forename: Ricardo
authors.initials: R
authors.lastname: Dorr
authors.forename: Luis E
authors.initials: LE
authors.lastname: Fernández
authors.forename: Francisco A
authors.initials: FA
authors.lastname: Uzal
authors.forename: Cristina
authors.initials: C
authors.lastname: Ibarra
mesh_terms: D001834:Body Water / Q000378:metabolism*; D003016:Clostridium perfringens; D003205:Computing Methodologies; D004847:Epithelial Cells; D006801:Humans; D008566:Membranes / Q000502:physiology*; D012984:Software