Activation of redox-systems of monocytes by hydrogen peroxide.
In this work the influence of H2O2 on the ability of human blood monocytes to generate ROS upon stimulation of cells by adhesion to glass surface and fMLP was studied using the luminol-dependent chemiluminescence (LDCL) method. Pretreatment of cells with H2O2 increased the adhesiveness of monocytes and ROS generation. Superoxide generation by cells in response to fMLP depended on the duration of pretreatment and the concentration of H2O2. The stimulatory effect on fMLP-induced LDCL of cells further depended on the Ca2+ concentration in the medium and on the activities of phospholipase A2, cyclooxygenases, and Mek1/2.
Krjukov
Alexei A
AA
Department of Biophysics, Belarus State University, Minsk, Belarus.
Semenkova
Galina N
GN
Cherenkevich
Sergei N
SN
Gerein
Valentin
V
D002122:Calcium Chloride / Q000378:metabolism; D002448:Cell Adhesion; D054513:Group IV Phospholipases A2; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry / Q000378:metabolism; D048369:MAP Kinase Kinase 1 / Q000378:metabolism; D048370:MAP Kinase Kinase 2 / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010084:Oxidation-Reduction; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
D017382:Reactive Oxygen Species; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008165:Luminol; D006861:Hydrogen Peroxide; D011451:Prostaglandin-Endoperoxide Synthases; D048369:MAP Kinase Kinase 1; D048370:MAP Kinase Kinase 2; D010741:Phospholipases A; D054513:Group IV Phospholipases A2; D054467:Phospholipases A2; D002122:Calcium Chloride