Functional nanofibrous scaffolds for bone reconstruction.
DNA compaction, encapsulation and stabilization strategies as well as a scheme for DNA chain stabilization by complex formation with modified fullerenes for gene delivery are discussed. DNA can be compacted in organic solvents and encapsulated with amphiphilic triblock copolymers. The rapid removal of the solvent mixtures by electrospinning together with a biodegradable polymer preserves the globular DNA conformation and can be used for bone reconstruction applications. Cationic fullerene surfactants can decorate and stabilize DNA coils in aqueous solution. The complex formation process is studied by static light scattering and analyzed in detail.
Burger
Christian
C
Chemistry Department, Stony Brook University, Stony Brook, NY 11794-3400, USA.
Chu
Benjamin
B
D000256:Adenoviridae / Q000235:genetics; D001672:Biocompatible Materials / Q000737:chemistry; D001704:Biopolymers / Q000737:chemistry; D001861:Bone Regeneration / Q000235:genetics / Q000502:physiology*; D002021:Buffers; D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D037741:Fullerenes / Q000737:chemistry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D019344:Lactic Acid / Q000737:chemistry; D008027:Light; D008956:Models, Chemical; D049329:Nanostructures / Q000737:chemistry* / Q000627:therapeutic use*; D009690:Nucleic Acid Conformation; D009930:Organic Chemicals / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D012542:Scattering, Radiation; D012997:Solvents / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D001704:Biopolymers; D002021:Buffers; D002412:Cations; D037741:Fullerenes; D009930:Organic Chemicals; D011091:Polyesters; D011108:Polymers; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C016240:polycaprolactone; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004247:DNA