title: Antiproliferative constituents from umbelliferae plants. IX. New triterpenoid glycosides from the fruits of Bupleurum rotundifolium.
abstract: The MeOH extract of the fruits of Bupleurum rotundifolium showed inhibitory activity against human gastric adenocarcinoma (MK-1) cell growth. Bioactivity-guided fractionation of the MeOH extract led to the isolation of four new triglycosides of 13beta,28-epoxy oleanane-type triterpenes, named rotundiosides O, Q, S and T; 12 new glycosides of oleanane-type triterpenes, named rotundiosides J-N, P, R, U-Y, and others; echinocystic acid 3-O-sulfate; and three known oleanane-type triterpene glycosides, rotundiosides A, F and G. The structures of the new isolates were determined based on chemical and spectroscopic evidence. The GI(50) of isolates against MK-1, HeLa and B16F10 cell lines are reported.
authors.affiliation: Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan. fujioka@fukuoka-u.ac.jp
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Fujioka
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yoshida
authors.forename: Hiroko
authors.initials: H
authors.lastname: Shibao
authors.forename: Tsuneatsu
authors.initials: T
authors.lastname: Nagao
authors.forename: Miyako
authors.initials: M
authors.lastname: Yoshida
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Matsunaga
authors.forename: Jiro
authors.initials: J
authors.lastname: Takata
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Karube
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Iwase
authors.forename: Hikaru
authors.initials: H
authors.lastname: Okabe
authors.forename: Kunihide
authors.initials: K
authors.lastname: Mihashi
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D029972:Bupleurum / Q000737:chemistry*; D045744:Cell Line, Tumor; D005638:Fruit / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006027:Glycosides; D014315:Triterpenes