Molecular typing of novel Rickettsia rickettsii isolates from Arizona.
Seven isolates of Rickettsia rickettsii were obtained from a skin biopsy, two whole-blood specimens, and from Rhipicephalus sanguineus ticks from eastern Arizona. Molecular typing of seven isolates of R. rickettsii and DNA samples from two other Rh. sanguineus ticks infected with R. rickettsii was conducted by PCR and DNA sequencing of rompA and 12 variable-number tandem repeat regions (VNTRs). All DNA specimens from Arizona were identical to each other and to reference human and Dermacentor andersoni isolates of R. rickettsii from Montana in their rOmpA gene sequences and 10 VNTRs. Two of the twelve VNTRs had differences in the number of repeat sequences in isolates from Arizona compared to those from Montana, thus conferring the novelty of the Rh. sanguineus-associated R. rickettsii.
Eremeeva
Marina E
ME
Viral and Rickettsial Zoonoses Branch, CDC, Atlanta, GA 30333, USA. MEremeeva@cdc.gov
Bosserman
Elizabeth
E
Zambrano
Maria
M
Demma
Linda
L
Dasch
Gregory A
GA
D001130:Arizona; D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012284:Rickettsia rickettsii / Q000235:genetics* / Q000302:isolation & purification*
D017931:DNA Primers; D004269:DNA, Bacterial