Application of femtosecond laser ablation for detaching grown protein crystals from glass capillary tube.
We developed a novel technique for detaching protein crystals from glass capillary tube using the counter diffusion crystallization technique by femtosecond laser irradiation. X-ray diffraction analysis demonstrated that femtosecond laser irradiation has little effect on crystallinity. This technique will contribute to progress in structural genomics as a powerful tool.
Kashii
Masafumi
M
Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. mkashii-osk@umin.ac.jp
Fujisawa
Ryo
R
Adachi
Hiroaki
H
Mori
Yusuke
Y
Sasaki
Takatomo
T
Koga
Yuichi
Y
Takano
Kazufumi
K
Kanaya
Shigenori
S
Matsumura
Hiroyoshi
H
Inoue
Tsuyoshi
T
Murakami
Satoshi
S
Sugamoto
Kazuomi
K
Yoshikawa
Hideki
H
D003460:Crystallization / Q000379:methods*; D005898:Glass; D007834:Lasers; D011506:Proteins / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure*; D013048:Specimen Handling / Q000379:methods*
D011506:Proteins