Facile separation and determination of Aconitine alkaloids in traditional Chinese medicines by CE with tris(2,2'-bipyridyl) ruthenium(II)-based electrochemiluminescence detection.
A facile CE method coupled with tris(2,2'-bipyridyl) ruthenium(II)-based electrochemiluminescence [Ru(bpy)(3) (2+)] detection was developed for simultaneous determination of Aconitum alkaloids, i.e., hypaconitine (HA), aconitine (AC), and mesaconitine (MA) in baseline separation. The optimal separation of these Aconitum alkaloids was achieved in a fused-silica capillary column (50 cm x 25 microm id) with 30 mM phosphate solution (pH 8.40) as running buffer at 12 kV applied voltage. The three alkaloids can be determined within 10 min by a single run. The calibration curves showed a linear range from 2.0 x 10(-7) to 2.0 x 10(-5) M for HA, 3.4 x 10(-7) to 1.7 x 10(-5) M for AC, and 3.8 x 10(-7) to 1.9 x 10(-5) M for MA. The RSDs for all analytes were below 3.01%. Good linear relationships were found with correlation coefficients for all analytes exceeding 0.993. The detection limits were 2.0 x 10(-8) M for HA, 1.7 x 10(-7) M for AC, and 1.9 x 10(-7) M for MA under optimal conditions. This method was successfully applied to determine the three alkaloids in Aconitum plants.
Yin
Jianyuan
J
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P. R. China.
Guo
Weiwei
W
Du
Yan
Y
Wang
Erkang
E
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000737:chemistry; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000302:isolation & purification*; D002138:Calibration; D056831:Coordination Complexes; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D005456:Fluorescent Dyes / Q000737:chemistry; D008163:Luminescent Measurements; D017975:Ruthenium Compounds
D015082:2,2'-Dipyridyl; D000470:Alkaloids; D056831:Coordination Complexes; D004365:Drugs, Chinese Herbal; D005456:Fluorescent Dyes; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds