title: Short report: Severe Plasmodium falciparum malaria in Cameroon: associated with the glutathione S-transferase M1 null genotype.
abstract: Glutathione S-transferases (GST) are a family of enzymes involved in phase-II detoxification of endogenous and xenobiotic compounds. Polymorphisms in GST genes have been associated with susceptibility to different diseases. In this study we determined the frequencies of polymorphisms in GSTM1, GSTT1, and GSTP1 in DNA of 138 children from Cameroon, presenting with uncomplicated malaria (N = 19), malaria with minor complications (N = 81), or severe malaria (N = 38). Analyses of GSTM1 and GSTT1 were performed using PCR-multiplex procedure, while GSTP1 was done by PCR-RFLP. Subjects presenting with malaria with complications were found more often of the GSTM1-null genotype (58-64%) as compared with those with uncomplicated malaria (32%), a difference that was statistically significant. We conclude that the GSTM1-null genotype is associated with malaria with complications.
authors.affiliation: Department of Pharmacology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
authors.forename: Reginald A
authors.initials: RA
authors.lastname: Kavishe
authors.forename: Jan B
authors.initials: JB
authors.lastname: Koenderink
authors.forename: Matthew B B
authors.initials: MB
authors.lastname: McCall
authors.forename: Wilbert H M
authors.initials: WH
authors.lastname: Peters
authors.forename: Bert
authors.initials: B
authors.lastname: Mulder
authors.forename: Cornelus C
authors.initials: CC
authors.lastname: Hermsen
authors.forename: Robert W
authors.initials: RW
authors.lastname: Sauerwein
authors.forename: Frans G M
authors.initials: FG
authors.lastname: Russel
authors.forename: Andre J A M
authors.initials: AJ
authors.lastname: Van der Ven
mesh_terms: D000818:Animals; D002163:Cameroon / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008288:Malaria / Q000150:complications / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D010963:Plasmodium falciparum / Q000235:genetics / Q000378:metabolism
chemical_list: D005982:Glutathione Transferase