Fast manipulation of cellular cAMP level by light in vivo.
The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACalpha and PACbeta. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells.
Schröder-Lang
Saskia
S
Max-Planck-Institut für Biophysik, 60439 Frankfurt, Germany.
Schwärzel
Martin
M
Seifert
Reinhard
R
Strünker
Timo
T
Kateriya
Suneel
S
Looser
Jens
J
Watanabe
Masakatsu
M
Kaupp
U Benjamin
UB
Hegemann
Peter
P
Nagel
Georg
G
D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D000242:Cyclic AMP / Q000032:analysis / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D004789:Enzyme Activation / Q000528:radiation effects; D005056:Euglena gracilis / Q000201:enzymology; D018014:Gene Transfer Techniques; D006801:Humans; D008027:Light; D013997:Time Factors; D014982:Xenopus laevis
D000242:Cyclic AMP; D000262:Adenylyl Cyclases