TNT transformation products are affected by the growth conditions of Raoultella terrigena.
High concentrations of 2,4,6-trinitrotoluene (TNT) and related nitroaromatic compounds are commonly found in soil and groundwater at former explosive plants. The bacterium, Raoultella terrigena strain HB, isolated from a contaminated site, converts TNT into the corresponding amino products. Radio-HPLC analysis with [(14)C]TNT identified aminodinitrotoluene, diaminonitrotoluene and azoxy-dimers as the main metabolites. Transformation rate and the type of metabolites that predominated in the culture medium and within the cells were significantly influenced by the culture conditions. The NAD(P)H-dependent enzymatic reduction of nitro-substituted compounds by cell-free extracts of R. terrigena was evaluated in vitro.
Claus
Harald
H
Johannes Gutenberg-University Mainz, Institute of Microbiology and Wine Research, Mainz, Germany. hclaus@uni-mainz.de
Perret
Nina
N
Bausinger
Tobias
T
Fels
Gregor
G
Preuss
Johannes
J
König
Helmut
H
D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D004755:Enterobacteriaceae / Q000254:growth & development* / Q000378:metabolism*; D014303:Trinitrotoluene / Q000378:metabolism*
D014303:Trinitrotoluene