Biocompatibility of furcal perforation repair material using cell culture technique: Ketac Molar versus ProRoot MTA.
OBJECTIVE
The objective of this study was to evaluate the cytotoxicity of furcal perforation repair materials, GI and MTA, using cell culture technique.


METHODS
The extract of ProRoot MTA and Ketac Molar were treated on PDL cells in a 96-well tissue-culture plate. Cell proliferation after an incubation period of 3 days was determined by using MTT assay.


RESULTS
The growth of cultured human periodontal fibroblast cells were suppressed by both perforation repair materials. The percent of cell viability in the Ketac Molar group was lower than in the ProRoot MTA group (P = .000).


CONCLUSIONS
Although Ketac Molar has the advantage of adhering to dentine, it is more cytotoxic to the PDL cells than MTA. In selecting the perforation repair material, it is recommended not only to consider the sealing ability of the material with dentine but also the biocompatibility of material to the underlying tissue.
Vajrabhaya
La-ongthong
LO
Department of Operative Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand. dtlvj@mahidol.ac.th <dtlvj@mahidol.ac.th>
Korsuwannawong
Suwanna
S
Jantarat
Jeeraphat
J
Korre
Sumeth
S
D017607:Aluminum Compounds / Q000633:toxicity*; D017610:Calcium Compounds / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004338:Drug Combinations; D005347:Fibroblasts / Q000187:drug effects; D005899:Glass Ionomer Cements / Q000633:toxicity*; D006801:Humans; D008422:Materials Testing; D010087:Oxides / Q000633:toxicity*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D018677:Tooth Injuries / Q000628:therapy*; D014092:Tooth Root / Q000293:injuries
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D005899:Glass Ionomer Cements; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C110712:ketac-molar; C086631:mineral trioxide aggregate