title: Effects of autonomic agonists and immunomodulatory cytokines on polymeric immunoglobulin receptor expression by cultured rat and human salivary and colonic cell lines.
abstract: OBJECTIVE
  Immunoglobulin A (IgA) is transported across glandular epithelial cells by polymeric immunoglobulin receptor (plgR), with each receptor molecule participating in only one round of transcytosis. Nerve-related stimuli rapidly increase salivary secretion of IgA, while concentrations are increased in the autoimmune disease Sjögren's syndrome. Our aim here was to determine whether autonomic agonists and cytokines present in Sjögren's-affected glands can up-regulate salivary cell plgR expression.
  
  
  METHODS
  Cultures of rat parotid acinar cells (PAR C5) and human submandibular gland ductal cells (HSG) were exposed to carbachol or adrenaline for 24 h and to interleukin-4 and/or interferon-gamma for 48 h. The human colonic cell line HT-29 served as a positive control for cytokine response. plgR mRNA was quantified by reverse transcription and real-time PCR and protein expression was examined by immunoblotting.
  
  
  RESULTS
  Carbachol increased plgR mRNA levels significantly in all cells but adrenaline did so only with PAR cells (P<0.05). HSG and HT-29 cells both up-regulated plgR gene transcription on exposure to interleukin-4 and interferon-gamma either alone or in combination (P<0.05). By contrast, production of plgR mRNA in PAR cells tended to decrease in response to all cytokine treatments. plgR protein levels rose in line with mRNA expression in cytokine-treated HT-29 cultures (P<0.05).
  
  
  CONCLUSIONS
  Autonomimetics can up-regulate plgR transcription in transformed and neoplastic salivary and colonic cells, although intracellular coupling mechanisms require further investigation. Immunomodulatory cytokines increased plgR expression in one of the salivary cell lines, but additional work is needed to establish whether this occurs in Sjögren's patients.
authors.affiliation: Salivary Research Unit, King's College London Dental Institute at Guy's, King's College and St Thomas' Hospitals, London, UK. stephen.cox@kcl.ac.uk
authors.forename: S W
authors.initials: SW
authors.lastname: Cox
authors.forename: L E
authors.initials: LE
authors.lastname: Ebersole
authors.forename: G H
authors.initials: GH
authors.lastname: Carpenter
authors.forename: G B
authors.initials: GB
authors.lastname: Proctor
mesh_terms: D000322:Adrenergic Agonists / Q000494:pharmacology; D000818:Animals; D001337:Autonomic Agents / Q000819:agonists*; D002217:Carbachol / Q000494:pharmacology; D002460:Cell Line; D018679:Cholinergic Agonists / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology*; D004837:Epinephrine / Q000494:pharmacology; D019073:HT29 Cells; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D010306:Parotid Gland / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D019056:Receptors, Polymeric Immunoglobulin / Q000032:analysis / Q000187:drug effects*; D018987:Salivary Ducts / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D012859:Sjogren's Syndrome / Q000276:immunology*; D013363:Submandibular Gland / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000322:Adrenergic Agonists; D001337:Autonomic Agents; D018679:Cholinergic Agonists; D016207:Cytokines; D012333:RNA, Messenger; D019056:Receptors, Polymeric Immunoglobulin; D015847:Interleukin-4; D007371:Interferon-gamma; D002217:Carbachol; D004837:Epinephrine