title: The nuclear proteome of chickpea (Cicer arietinum L.) reveals predicted and unexpected proteins.
abstract: Nuclear proteins constitute a highly organized, complex network that plays diverse roles during cellular development and other physiological processes. The yeast nuclear proteome corresponds to about one-fourth of the total cellular proteins, suggesting the involvement of the nucleus in a number of diverse functions. In an attempt to understand the complexity of plant nuclear proteins, we have developed a proteome reference map of a legume, chickpea, using two-dimensional gel electrophoresis (2-DE). Approximately, 600 protein spots were detected, and LC-ESI-MS/MS analyses led to the identification of 150 proteins that have been implicated in a variety of cellular functions. The largest percentage of the identified proteins was involved in signaling and gene regulation (36%), while 17% were involved in DNA replication and transcription. The chickpea nuclear proteome indicates the presence of few new nuclear proteins of unknown functions vis-à-vis many known resident proteins. To the best of our knowledge, this is the first report of a nuclear proteome of an unsequenced genome.
authors.affiliation: National Centre for Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Aarti
authors.initials: A
authors.lastname: Pandey
authors.forename: Mani Kant
authors.initials: MK
authors.lastname: Choudhary
authors.forename: Deepti
authors.initials: D
authors.lastname: Bhushan
authors.forename: Arnab
authors.initials: A
authors.lastname: Chattopadhyay
authors.forename: Subhra
authors.initials: S
authors.lastname: Chakraborty
authors.forename: Asis
authors.initials: A
authors.lastname: Datta
authors.forename: Niranjan
authors.initials: N
authors.lastname: Chakraborty
mesh_terms: D002853:Chromatography, Liquid; D029863:Cicer / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D009687:Nuclear Proteins / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D009687:Nuclear Proteins; D010940:Plant Proteins