title: Validation of a Rickettsia prowazekii-specific quantitative real-time PCR cassette and DNA extraction protocols using experimentally infected lice.
abstract: The presence of R. prowazekii in lice using R. prowazekii-specific qPCR cassettes showed the changes of the rickettsial burden in the lice post-infection.
authors.affiliation: NMRC-IDD/RDD, 503 Robert Grant Ave., Silver Spring, MD 20910-7500, USA. RozmajzlP@nmrc.navy.mil
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Rozmajzl
authors.forename: Linda
authors.initials: L
authors.lastname: Houhamdi
authors.forename: Ju
authors.initials: J
authors.lastname: Jiang
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.forename: Allen L
authors.initials: AL
authors.lastname: Richards
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004195:Disease Models, Animal; D008003:Phthiraptera / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012283:Rickettsia prowazekii / Q000235:genetics* / Q000302:isolation & purification; D014438:Typhus, Epidemic Louse-Borne / Q000175:diagnosis*
chemical_list: D004269:DNA, Bacterial