Spectrophotometric determination of aminomethylbenzoic acid using sodium 1,2-naphthoquinone-4-sulfonate as the chemical derivative chromogenic reagent.
A new method has been established for the determination of aminomethylbenzoic acid using sodium 1,2-naphthoquinone-4-sulfonate as the chemical derivative chromogenic reagent. This method is based on the formation of a pink compound from the reaction of aminomethylbenzoic acid and sodium 1,2-naphthoquinone-4-sulfonate. The nucleophilic substitution reaction proceeds quantitatively in pH 12.0 buffer solution. The stoichiometric ratio of the reaction, maximum absorption wavelength and the value of epsilon(430) were 1:1, 430 nm, and 2.87 x 10(3)L mol(-1)cm(-1), respectively. Beer's law was obeyed in the range of 0.80-80 mg/L of aminomethylbenzoic acid. The data have been filled to a linear regression equation A=0.03183+0.01658C (mg/L), with a correlation coefficient of 0.9996. The detection limit is 0.11 mg/L, R.S.D. is 0.54%, and average recovery is over 99.6%. This paper further improves the determination of aminomethylbenzoic acid compared to the previous methods. The kinetic property and reaction mechanism have also been discussed. This proposed method has been successfully applied to the determination of aminomethylbenzoic acid in injection of aminomethylbenzoic acid with satisfactory results.
Li
Quan-Min
QM
College of Chemistry and Environmental Science, Henan Normal University, Henan Key Laboratory for Environmental Pollution Control, Xinxiang, Henan 45300, PR China. mercury6068@hotmail.com
Yang
Zhan-Jun
ZJ
D010129:4-Aminobenzoic Acid / Q000652:urine; D002138:Calibration; D002863:Chromogenic Compounds / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009285:Naphthoquinones / Q000737:chemistry*; D015203:Reproducibility of Results; D012996:Solutions / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D062366:para-Aminobenzoates
D002863:Chromogenic Compounds; D009285:Naphthoquinones; D012996:Solutions; D012997:Solvents; D013501:Surface-Active Agents; D062366:para-Aminobenzoates; C011942:1,2-naphthoquinone-4-sulfonate; C015668:4-aminomethylbenzoic acid; D010129:4-Aminobenzoic Acid