[Establishment of miniCTTA multiplex set with fluorescence-labeled primers].
OBJECTIVE
To establish a miniCTTA multiplex set including short tandem repeat (STR) markers as CSF1PO, TH01, and TPOX and amelogenin gene, whose amplified fragments are shorter than 130 bp.


METHODS
The length of the gene fragments amplified with fluorescence-labeled primers were analyzed by ABI3100 Genetic Analyzer for genotyping of 100 unrelated individuals, 10 genealogies and 30 highly degraded specimens.


RESULTS
The genotypes derived form miniCTTA were consistent with the results by AmpFLSTR Identifiler kit.


CONCLUSIONS
MiniCTTA multiplex set is useful for personal identification and paternity test, especially applicable for degraded DNA sample.
Liu
Chao
C
Institute of Forensic Medicine, Southern Medical University, Guangzhou 510515, China. liuchaogz@21cn.com
Wang
Hui-pin
HP
Sun
Hong-yu
HY
Wang
Hui-jun
HJ
D053523:Amelogenin / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D016172:DNA Fingerprinting; D017931:DNA Primers; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D015203:Reproducibility of Results
D053523:Amelogenin; D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA