title: Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family.
abstract: Matrine, one of the main active components from the dry roots of Sophora flavescence, was known to induce apoptosis in a variety of tumor cells in vitro. However, the molecular mechanism of cell apoptosis induced by Matrine remains elusive. Here, we investigated the apoptosis in Matrine-treated human gastric cancer MKN45 cells. The results showed that Matrine could inhibit cell proliferation and induce apoptosis in a dose-dependent manner. Further immunoblots revealed that in Matrine-treated cells, caspase-3, -7 were activated and the pro-apoptotic molecules Bok, Bak, Bax, Puma, and Bim were also up-regulated. Our results suggested that Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family.
authors.affiliation: Cancer Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Luo
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Tiejun
authors.initials: T
authors.lastname: Jiang
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Lu
authors.forename: Wo
authors.initials: W
authors.lastname: Zhang
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Jing
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Linrong
authors.initials: L
authors.lastname: Pang
authors.forename: Kangjie
authors.initials: K
authors.lastname: Chen
authors.forename: Fuming
authors.initials: F
authors.lastname: Qiu
authors.forename: Xiuyan
authors.initials: X
authors.lastname: Yu
authors.forename: Jiahui
authors.initials: J
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
mesh_terms: D000470:Alkaloids / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011807:Quinolizines / Q000633:toxicity*; D013274:Stomach Neoplasms; D000093842:Matrines
chemical_list: D000470:Alkaloids; D019253:Proto-Oncogene Proteins c-bcl-2; D011807:Quinolizines; D053148:Caspase 3; D053179:Caspase 7; D000093842:Matrines