Generating vesicular stomatitis virus pseudotype bearing the severe acute respiratory syndrome coronavirus spike envelope glycoprotein for rapid and safe neutralization test or cell-entry assay.
We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Delta G*SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARS-CoV in trans. The neutralization and infection blocking tests showed that the VSV Delta G*SG and SARS-CoV reacted similarly to SARS-CoV specific antiserum, suggesting the VSVDelta G*SG can be a safe replacement of the live SARS-CoV for neutralization test and cell-entry assay.
Ge
Jinying
J
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R. China.
Wen
Zhiyun
Z
Wang
Xijun
X
Hu
Sen
S
Liu
Yonggang
Y
Kong
Xiangang
X
Chen
Hualan
H
Bu
Zhigao
Z
D000818:Animals; D002645:Chickens; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D008562:Membrane Glycoproteins / Q000276:immunology*; D009500:Neutralization Tests / Q000592:standards / Q000662:veterinary; D018353:Rhabdoviridae Infections / Q000276:immunology / Q000662:veterinary*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D012449:Safety; D013047:Specific Pathogen-Free Organisms; D064370:Spike Glycoprotein, Coronavirus; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins