title: Hepatic intra-arterial injection of 3-bromopyruvate in rabbit VX2 tumor.
abstract: OBJECTIVE
  To evaluate the antitumoral effects of an intra-arterial injection of 3-bromopyruvate (3-BrPA) on liver VX2 tumor in rabbits.
  
  
  METHODS
  Twenty rabbits with surgically implanted liver VX2 tumors were used. The rabbits were divided into three groups: a control, a saline, and a 3-BrPA group. Four rabbits were not treated at all, and they served as the control group. The saline group (n = 6) received only intra-arterial saline injection. The 3-BrPA group (n = 10) received an intra-arterial injection of 3-bromopyruvate through the hepatic artery. The delivered amounts of 3-bromopyruvate were as follows: 25 ml of 0.5 mM in six rabbits, 25 ml of 1.0 mM in two rabbits, and 25 ml of 2.0 mM in two rabbits. Four days after intra-arterial injection, the rabbits were sacrificed and histopathologic analysis of the explanted livers was performed with comparison of the tumor necrosis ratio (a percentage of the necrotic area versus the entire tumorous area) in each group.
  
  
  RESULTS
  The mean tumor necrosis ratio was 12.5+/-4.2%, 44.8+/-24.7%, and 49.4+/-14.3% in the control, saline, and 3-BrPA groups, respectively. Between the control and the saline group, and between the control and the 3-BrPA group the mean tumor necrosis ratio appeared to be significantly different (P<0.05). However, there was no statistical difference in the mean tumor necrosis ratio between the saline and the 3-BrPA group (P = 0.416).
  
  
  CONCLUSIONS
  A single session of intra-arterial injection of 3-BrPA showed no better results in terms of tumor necrosis than that of saline injection in a rabbit VX2 tumor model.
authors.affiliation: Department of Radiology, Kangwon National University College of Medicine, Kangwon-do, Korea.
authors.forename: S W
authors.initials: SW
authors.lastname: Shin
authors.forename: H
authors.initials: H
authors.lastname: Han
authors.forename: S W
authors.initials: SW
authors.lastname: Choo
authors.forename: B C
authors.initials: BC
authors.lastname: Yoo
authors.forename: C K
authors.initials: CK
authors.lastname: Park
authors.forename: Y S
authors.initials: YS
authors.lastname: Do
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: D H
authors.initials: DH
authors.lastname: Lee
authors.forename: D
authors.initials: D
authors.lastname: Choi
authors.forename: I-W
authors.initials: IW
authors.lastname: Choo
authors.forename: S S
authors.initials: SS
authors.lastname: Kim
authors.forename: J Y
authors.initials: JY
authors.lastname: Lee
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D006499:Hepatic Artery; D007269:Injections, Intra-Arterial; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy*; D009368:Neoplasm Transplantation; D011773:Pyruvates / Q000008:administration & dosage / Q000494:pharmacology*; D011817:Rabbits; D018709:Statistics, Nonparametric
chemical_list: D004791:Enzyme Inhibitors; D011773:Pyruvates; C017092:bromopyruvate