Molecular anatomy of a trafficking organelle.
Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.
Takamori
Shigeo
S
Department of Neurobiology, Max-Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany.
Holt
Matthew
M
Stenius
Katinka
K
Lemke
Edward A
EA
Grønborg
Mads
M
Riedel
Dietmar
D
Urlaub
Henning
H
Schenck
Stephan
S
Brügger
Britta
B
Ringler
Philippe
P
Müller
Shirley A
SA
Rammner
Burkhard
B
Gräter
Frauke
F
Hub
Jochen S
JS
De Groot
Bert L
BL
Mieskes
Gottfried
G
Moriyama
Yoshinori
Y
Klingauf
Jürgen
J
Grubmüller
Helmut
H
Heuser
John
J
Wieland
Felix
F
Jahn
Reinhard
R
D000818:Animals; D008055:Lipids / Q000737:chemistry; D008958:Models, Molecular; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry; D051381:Rats; D013050:Spectrometry, Fluorescence; D013572:Synaptic Vesicles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
D008055:Lipids; D011506:Proteins