1,25-Dihydroxyvitamin D3 regulates genes responsible for detoxification in intestine.
1Alpha,25-Dihydroxyvitamin D(3) (1,25-(OH)(2)D(3)), the biologically active form of vitamin D(3), not only plays a major role in mammalian calcium and phosphorous homeostasis but also exerts pleiotropic effects on cell proliferation, differentiation and the immune system. Further, vitamin D is believed to play a significant role in the prevention of colon, prostate, and breast cancer and in reducing the risk of autoimmune diseases. To gain insight into the mechanism whereby vitamin D can have such diverse actions, we have employed microarray technology. We studied the effect of a single dose of 1,25-(OH)(2)D(3) on gene expression in the intestine of vitamin D-deficient rats. Within 6 h, 1,25-(OH)(2)D(3) stimulates the expression of several phase I and phase II biotransformation genes. There is also an increased expression of antioxidant genes. These results support the idea that vitamin D is a significant factor in detoxification and protection against environmental toxins.
Kutuzova
Galina D
GD
Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706-1544, USA.
DeLuca
Hector F
HF
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001711:Biotransformation; D002117:Calcitriol / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004386:Duodenum / Q000187:drug effects* / Q000201:enzymology*; D004851:Epoxide Hydrolases / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism; D008658:Inactivation, Metabolic / Q000235:genetics*; D008297:Male; D050216:Metabolic Detoxication, Phase I / Q000235:genetics; D050217:Metabolic Detoxication, Phase II / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013250:Steroid Hydroxylases / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014166:Transferases / Q000235:genetics / Q000378:metabolism; D014808:Vitamin D Deficiency; D014815:Vitamins / Q000494:pharmacology*
D000975:Antioxidants; D012333:RNA, Messenger; D014815:Vitamins; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D006419:Heme Oxygenase (Decyclizing); D014166:Transferases; D004851:Epoxide Hydrolases; D002117:Calcitriol