Electrochemical biosensor for catechol using agarose-guar gum entrapped tyrosinase.
An electrochemical biosensor using tyrosinase was constructed for the determination of catechol. The enzyme was extracted from a plant source Amorphophallus companulatus and entrapped in agarose-guar gum composite biopolymer matrix. Catechol was determined by direct reduction of biocatalytically liberated quinone species at -0.1 V versus Ag/AgCl (3M KCl). The response was found to be linear and concentration dependent in the range of 6 x 10(-5) to 8 x 10(-4)M with a lower detection limit of 6 microM. It has reusability up to 20 cycles and a shelf life of more than 2 months when stored at 4 degrees C.
Tembe
Sanket
S
Department of Chemistry, University of Pune, Ganeshkhind, Pune 411 007, India.
Inamdar
Shaukat
S
Haram
Santosh
S
Karve
Meena
M
D'Souza
S F
SF
D016227:Benzoquinones / Q000378:metabolism; D001704:Biopolymers / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D002396:Catechols / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D004784:Environmental Monitoring / Q000295:instrumentation; D005685:Galactans; D008351:Mannans; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D053149:Plant Gums; D012680:Sensitivity and Specificity
D016227:Benzoquinones; D001704:Biopolymers; D002396:Catechols; D005685:Galactans; D008351:Mannans; D053149:Plant Gums; C004532:quinone; C007894:guar gum; D014442:Monophenol Monooxygenase; C034221:catechol