title: Application of RNA interference for inhibiting the replication of feline immunodeficiency virus in chronically infected cell lines.
abstract: RNA interference (RNAi) is a process in which double-stranded RNA induces the post-transcriptional sequence-specific degradation of homologous messenger RNA. The present study was carried out to apply the RNAi technology to inhibit the replication of feline immunodeficiency virus (FIV). Four small interfering RNAs (siRNAs) homologous to the FIV gag gene were synthesized and transfected into a feline fibroblastic cell line chronically infected with FIV (CRFK/FIV). These synthetic siRNAs efficiently inhibited the replication of FIV. Next, we examined the effect of retroviral vector-mediated transfer of FIV-specific short hairpin RNA (shRNA) on the replication of FIV in a feline T-cell line chronically infected with FIV (FL4). The retroviral vector-mediated transfer of FIV-specific shRNA was shown to markedly inhibit the replication of FIV in the FL4 cells. These results provide useful information for the development of RNAi-based gene therapy strategy to control FIV infection.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Tokyo, Japan. kbaba@virus.kyoto-u.ac.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Baba
authors.forename: Fuminori
authors.initials: F
authors.lastname: Mizukoshi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Goto-Koshino
authors.forename: Asuka
authors.initials: A
authors.lastname: Setoguchi-Mukai
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Fujino
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000517:prevention & control* / Q000821:virology; D002415:Cats; D002460:Cell Line; D017931:DNA Primers / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D015750:Genes, gag / Q000235:genetics; D016089:Immunodeficiency Virus, Feline / Q000187:drug effects* / Q000502:physiology; D016180:Lentivirus Infections / Q000517:prevention & control / Q000662:veterinary*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry / Q000494:pharmacology*; D012190:Retroviridae / Q000502:physiology; D013997:Time Factors; D014162:Transfection / Q000662:veterinary; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D017931:DNA Primers; D034741:RNA, Small Interfering