title: Steel factor controls midline cell death of primordial germ cells and is essential for their normal proliferation and migration.
abstract: During germ-cell migration in the mouse, the dynamics of embryo growth cause many germ cells to be left outside the range of chemoattractive signals from the gonad. At E10.5, movie analysis has shown that germ cells remaining in the midline no longer migrate directionally towards the genital ridges, but instead rapidly fragment and disappear. Extragonadal germ cell tumors of infancy, one of the most common neonatal tumors, are thought to arise from midline germ cells that failed to die. This paper addresses the mechanism of midline germ cell death in the mouse. We show that at E10.5, the rate of apoptosis is nearly four-times higher in midline germ cells than those more laterally. Gene expression profiling of purified germ cells suggests this is caused by activation of the intrinsic apoptotic pathway. We then show that germ cell apoptosis in the midline is activated by down-regulation of Steel factor (kit ligand) expression in the midline between E9.5 and E10.5. This is confirmed by the fact that removal of the intrinsic pro-apoptotic protein Bax rescues the germ-cell apoptosis seen in Steel null embryos. Two interesting things are revealed by this: first, germ-cell proliferation does not take place in these embryos after E9.0; second, migration of germ cells is highly abnormal. These data show first that changing expression of Steel factor is required for normal midline germ cell death, and second, that Steel factor is required for normal proliferation and migration of germ cells.
authors.affiliation: Division of Developmental Biology, Cincinnati Children's Hospital Research Foundation, Cincinnati, OH 45229, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Runyan
authors.forename: Kyle
authors.initials: K
authors.lastname: Schaible
authors.forename: Kathleen
authors.initials: K
authors.lastname: Molyneaux
authors.forename: Zhuoqiao
authors.initials: Z
authors.lastname: Wang
authors.forename: Linda
authors.initials: L
authors.lastname: Levin
authors.forename: Christopher
authors.initials: C
authors.lastname: Wylie
mesh_terms: D000818:Animals; D017209:Apoptosis; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005835:Genitalia / Q000166:cytology / Q000196:embryology; D005854:Germ Cells / Q000166:cytology*; D006066:Gonads / Q000166:cytology / Q000196:embryology; D049452:Green Fluorescent Proteins; D008297:Male; D008643:Mesentery / Q000196:embryology; D051379:Mice; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism; D019089:Stem Cell Factor / Q000235:genetics / Q000502:physiology*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D019089:Stem Cell Factor; D051028:bcl-2-Associated X Protein; D049452:Green Fluorescent Proteins; D019009:Proto-Oncogene Proteins c-kit