title: Atomic force microscopy investigation of Mason-Pfizer monkey virus and human immunodeficiency virus type 1 reassembled particles.
abstract: Particles of DeltaProCANC, a fusion of capsid (CA) and nucleocapsid (NC) protein of Mason-Pfizer monkey virus (M-PMV), which lacks the amino terminal proline, were reassembled in vitro and visualized by atomic force microscopy (AFM). The particles, of 83-84 nm diameter, exhibited ordered domains based on trigonal arrays of prominent rings with center to center distances of 8.7 nm. Imperfect closure of the lattice on the spherical surface was affected by formation of discontinuities. The lattice is consistent only with plane group p3 where one molecule is shared between contiguous rings. There are no pentameric clusters nor evidence that the particles are icosahedral. Tubular structures were also reassembled, in vitro, from two HIV fusion proteins, DeltaProCANC and CANC. The tubes were uniform in diameter, 40 nm, but varied in length to a maximum of 600 nm. They exhibited left handed helical symmetry based on a p6 hexagonal net. The organization of HIV fusion proteins in the tubes is significantly different than for the protein units in the particles of M-PMV DeltaProCANC.
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California, Irvine, 560 SH, Irvine, CA 92697-3900, USA.
authors.forename: Yu G
authors.initials: YG
authors.lastname: Kuznetsov
authors.forename: P
authors.initials: P
authors.lastname: Ulbrich
authors.forename: S
authors.initials: S
authors.lastname: Haubova
authors.forename: T
authors.initials: T
authors.lastname: Ruml
authors.forename: A
authors.initials: A
authors.lastname: McPherson
mesh_terms: D006678:HIV / Q000648:ultrastructure*; D006801:Humans; D046911:Macromolecular Substances; D016093:Mason-Pfizer monkey virus / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D014764:Viral Proteins / Q000302:isolation & purification / Q000378:metabolism; D014771:Virion / Q000648:ultrastructure*; D019065:Virus Assembly
chemical_list: D046911:Macromolecular Substances; D014764:Viral Proteins