title: In vitro studies on the release of isoniazid incorporated in poly(epsilon-caprolactone).
abstract: A polymeric micro- and nanosphere formulation using poly (epsilon-caprolactone) (PCL) to entrap an antituberculosis drug, isoniazid (INH), was developed and characterized. The microspheres were prepared by a solvent evaporation method using ethyl acetate, PCL and INH as the organic phase and water and Tween 40 as the aqueous phase. The nanospheres were prepared by a spontaneous emulsification solvent diffusion method using 40% ethanol in acetone (v/v), PCL and INH as the organic phase and water and Tween 40 as the aqueous phase. After freeze-drying, these systems were characterized by scanning electron microscopy (SEM), particle size analysis, determination of entrapped INH content, in vitro INH release and brine shrimp toxicity bioassay.
authors.affiliation: Instituto de Química, Biological Chemistry Laboratory, Universidade Estadual de Campinas, C.P. 6159, Campinas CEP 13083-970, S.P., Brazil. duran@iqm.unicamp.br
authors.forename: N
authors.initials: N
authors.lastname: Durán
authors.forename: A F
authors.initials: AF
authors.lastname: De Oliveira
authors.forename: M M M
authors.initials: MM
authors.lastname: De Azevedo
mesh_terms: D000818:Animals; D000904:Antibiotics, Antitubercular / Q000493:pharmacokinetics; D001156:Artemia / Q000187:drug effects; D002208:Caproates / Q000737:chemistry / Q000493:pharmacokinetics*; D003692:Delayed-Action Preparations / Q000493:pharmacokinetics; D004339:Drug Compounding / Q000379:methods*; D016503:Drug Delivery Systems; D007538:Isoniazid / Q000493:pharmacokinetics*; D007783:Lactones / Q000737:chemistry / Q000493:pharmacokinetics*; D008863:Microspheres; D043942:Nanotubes / Q000737:chemistry; D010316:Particle Size; D011108:Polymers / Q000737:chemistry / Q000493:pharmacokinetics; D018675:Toxicity Tests
chemical_list: D000904:Antibiotics, Antitubercular; D002208:Caproates; D003692:Delayed-Action Preparations; D007783:Lactones; D011108:Polymers; C121056:caprolactone; D007538:Isoniazid