N-linked oligosaccharides on the low density lipoprotein receptor homolog SorLA/LR11 are modified with terminal GalNAc-4-SO4 in kidney and brain.
Sorting protein-related receptor (SorLA/LR11) is a highly conserved mosaic receptor that is expressed by cells in a number of different tissues including principal cells of the collecting ducts in the kidney and neurons in the central and peripheral nervous systems. SorLA/LR11 has features that indicate it serves as a sorting receptor shuttling between the plasma membrane, endosomes, and the Golgi. We have found that a fraction of SorLA/LR11 that is synthesized in the kidney and the brain bears N-linked oligosaccharides that are modified with terminal beta1,4-linked GalNAc-4-SO(4). Oligosaccharides located in the vacuolar sorting (Vps) 10p domain (Vps10p domain) are modified with beta1,4-linked GalNAc when the Vps10p domain is expressed in cells along with either of two recently cloned protein-specific beta1,4GalNAc-transferases, GalNAcTIII and GalNAcTIV. Either of two sequences with basic amino acids located within the Vps10p domain is able to mediate recognition by these beta1,4GalNAc-transferases. The highly specific modification of oligosaccharides in the Vps10p domain of SorLA/LR11 with terminal GalNAc-4-SO(4) suggests that this unusual modification may modulate the interaction of SorLA/LR11 with proteins and influence their trafficking.
Fiete
Dorothy
D
Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Mi
Yiling
Y
Oats
Edward L
EL
Beranek
Mary C
MC
Baenziger
Jacques U
JU
D000116:Acetylgalactosamine / Q000031:analogs & derivatives* / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D011973:Receptors, LDL / Q000737:chemistry* / Q000502:physiology; D013463:Sulfuric Acid Esters / Q000737:chemistry*
D006023:Glycoproteins; D026901:Membrane Transport Proteins; D009844:Oligosaccharides; D011973:Receptors, LDL; C491168:Sorl1 protein, mouse; D013463:Sulfuric Acid Esters; C504597:phenyl 2-acetamido-2-deoxy-4-O-sulfonatogalactopyranoside; D000116:Acetylgalactosamine