[Comparative study on accumulation of Ah-receptor agonists in contaminated soil based on EROD bioassay and chemical analysis].
The potential ecological risk by wastewater or reclaimed water for irrigation is of great concerns in recent years, but little work was done on the chronic toxicities through long term accumulation of persistent organic chemicals in soil. In present work, concentration of Ah-receptor agonists in soil organic extract was measured by an ethoxyresorfin O-deethylase (EROD) bioassay, which was calibrated and expressed by the 2, 3, 7, 8-tetrachlorodibenzo- p-dioxin (2, 3, 7, 8-TCDD) toxic equivalent (TEQbio). Simultaneously, 16 PAHs in soil were analyzed and their TEQs (total as TEQ(PAHs)) were calculated according to their toxic equivalent factors (TEFs) cited from literature. By bioassay, it was found that the concentration level of Ah-receptor agonists in soil irrigated using reclaimed water could be as high as 97.4 ng/kg, which was obviously higher than that in background soil using ground water irrigation regime (56.0 ng/kg). In comparing the results from bioassay and chemical analysis, the percentage of TEQ(PAHs) in TEQbio increased from 10.3% in background soil to 78.6% in the soil irrigated by reclaimed water. Use of reclaimed water for irrigation could result in the accumulation of Ah-receptor agonists in soil,and a major part of them in this case could be attributed to the accumulation of 16 priority PAHs in soils.
Yu
Guo
G
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. yuguodaxia@126.com
Wu
Wen-yong
WY
Liu
Hong-lu
HL
Xiao
Rui-yang
RY
Wang
Chun-xia
CX
Wang
Zi-jian
ZJ
D000818:Animals; D001681:Biological Assay / Q000379:methods; D045744:Cell Line, Tumor; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D000072317:Polychlorinated Dibenzodioxins / Q000032:analysis* / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists*; D012989:Soil Pollutants / Q000032:analysis* / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity
D000072317:Polychlorinated Dibenzodioxins; D011084:Polycyclic Aromatic Hydrocarbons; D018336:Receptors, Aryl Hydrocarbon; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1