Pancreatic autoimmunity induction with insulin B:9-23 peptide and viral mimics in the NZB mouse.
To create a new experimental model of diabetes, we used the New Zealand Black (NZB) mouse as a potential model. NZB mice were immunized with B:9-23 insulin peptide in IFA and the viral mimic, poly(A:U). No diabetes was observed but blood glucose was significantly higher in the B:9-23 peptide group compared to controls. Insulin autoantibodies (IAA) were only induced in groups given the B:9-23 peptide. B:9-23 alone induced peri-insulitis. We demonstrate insulin autoimmunity in the NZB mouse using the insulin peptide B:9-23 and viral mimics. The reason for the protection from diabetes despite the presence of autoimmunity is currently not established.
Devendra
Devasenan
D
Barbara Davis Center for Childhood Diabetes, P.O. Box 6511, Aurora, CO 80010, USA.
Miao
Dongmei
D
Nakayama
Maki
M
Eisenbarth
George S
GS
Liu
Edwin
E
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D015551:Autoimmunity / Q000187:drug effects*; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005500:Follow-Up Studies; D007328:Insulin / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000276:immunology / Q000473:pathology; D051379:Mice; D008817:Mice, Mutant Strains; D010446:Peptide Fragments / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D011063:Poly A-U / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors
D000276:Adjuvants, Immunologic; D001786:Blood Glucose; D007328:Insulin; D010446:Peptide Fragments; D011063:Poly A-U