Highly sensitive HLA-DNA typing from formalin-fixed and paraffin-embedded tissue samples.
Nucleotide sequences have been determined for more than 1700 different alleles at the core of the human leukocyte antigen (HLA) system. The highly polymorphic character of these genes affects adaptive immune response and is also useful for forensic applications. HLA typing from formalin-fixed and paraffin-embedded tissue provides abundant useful information for both clinical settings and forensic investigations. This study, which investigated the potential use of DNA from formalin-fixed and paraffin-embedded tissue samples in an HLA PCR sequence-specific primer and probe (SPP) system, showed that tissue fixed in formalin for less than 3 days and embedded in paraffin can serve as a useful source of DNA for PCR-SPP typing kits.
Ota
Masao
M
Department of Legal Medicine, Shinshu University School of Medicine, Asahi, Matsumoto, Japan. otamasao@sch.md.shinshu-u.ac.jp
Shimada
Kazunori
K
Asamura
Hideki
H
Katsuyama
Yoshihiko
Y
Fukushima
Hirofumi
H
D001344:Autopsy; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D005554:Forensic Medicine; D005557:Formaldehyde; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D017931:DNA Primers; D006680:HLA Antigens; D005557:Formaldehyde; D004247:DNA