The effect of systemic prednisolone on propylene glycol-induced otitis media in guinea pig.
OBJECTIVE
In this histopathologic study, we investigated the effect of systemic prednisolone on propylene glycol-induced otitis media in guinea pig ear.


METHODS
Ten adult guinea pigs received systemic prednisolone (10 mg, IM, single dose) together with a single application of 60% propylene glycol (0.2 ml, per ear) to both middle ears. Ten control guinea pigs were given a single application of 60% propylene glycol (0.2 ml) in the left ear, and a single application of saline (0.2 ml) in the right ear. Four weeks later, the animals were sacrificed and temporal bones were removed and prepared for histological evaluation. Paraffin-embedded specimens were horizontally sectioned at 7 microns. One of each five sections was stained with hematoxylin and eosin, and studied under light microscopy.


RESULTS
In the control group, otitis media developed in six of nine propylene glycol-administered ears (66%), whereas all saline-treated ears remained intact. In the prednisolone group, only one (6%) of 16 ears developed otitis media and cholesteatoma. The difference was statistically significant.


CONCLUSIONS
Our results suggest that systemic prednisolone has a potent effect to prevent propylene glycol-induced otitis media in guinea pig ears.
Kayhan
Fatma Tülin
FT
Department of Otolaryngology, Dr. Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey. ftulinkayhan@hotmail.com
Algün
Zeynep
Z
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007273:Injections, Intramuscular; D010033:Otitis Media / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D011239:Prednisolone / Q000008:administration & dosage*; D019946:Propylene Glycol
D000893:Anti-Inflammatory Agents; D019946:Propylene Glycol; D011239:Prednisolone