title: Nitrogen removal from wastewater using simultaneous nitrate reduction and anaerobic ammonium oxidation in single reactor.
abstract: The effects of C/N ratio and total organic carbon (TOC) loading on nitrogen removal through simultaneous nitrate reduction and anaerobic ammonium oxidation in a single reactor were examined. Granular sludge taken from a methane fermentation reactor was placed in an upflow reactor and supplied with synthetic wastewater containing nitrate at a C/N ratio of 1 to grow heterotrophic denitrifying bacteria. When nitrogen removal ratio reached 30%, anammox sludge attached to nonwoven-carrier was added into the same reactor and then ammonia was added to the synthetic wastewater. Nitrogen removal ratio was markedly increased to 80-94%. In this system, nitrogen removal ratio was affected by C/N ratio and TOC loading, not by the amount of granular sludge. A stable isotopic analysis using 15N-labeled nitrate showed that N2 gas was formed by anammox reaction.
authors.affiliation: Matsudo Research Laboratory, Hitachi Plant Technologies, 537 Kami-Hongo, Matsudo-shi, Chiba 271-0064, Japan. tatsuo.sumino.cj@hitachi-pt.com
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Sumino
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Isaka
authors.forename: Hajime
authors.initials: H
authors.lastname: Ikuta
authors.forename: Yuko
authors.initials: Y
authors.lastname: Saiki
authors.forename: Toyokazu
authors.initials: T
authors.lastname: Yokota
mesh_terms: D000693:Anaerobiosis; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007220:Industrial Waste / Q000517:prevention & control; D009566:Nitrates / Q000378:metabolism*; D009584:Nitrogen / Q000302:isolation & purification / Q000493:pharmacokinetics*; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000493:pharmacokinetics*; D018508:Water Purification / Q000379:methods*
chemical_list: D007220:Industrial Waste; D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D012722:Sewage; D014874:Water Pollutants, Chemical; D009584:Nitrogen