Circulating tumoral cells lack circadian-rhythm in hospitalized metastasic breast cancer patients.
BACKGROUND
The relationship between breast cancer and circadian rhythm variation has been extensively studied. Increased breast tumorigenesis has been reported in melatonin-suppressed experimental models and in observational studies.


OBJECTIVE
Circulating Tumor Cells (CTC) circadian- rhythm may optimize the timing of therapies. This is a prospective experimental study to ascertain the day-time and night-time CTC levels in hospitalized metastasic breast cancer (MBC) patients.


METHODS
CTC are isolated and enumerated from a 08:00 AM and 08:00 PM blood collections. 23 MBC and 23 healthy volunteers entered the study. 69 samples were collected (23 samples at 08:00 AM and 23 samples at 08:00 PM from MBC; 23 samples from healthy volunteers). Results from two patients were rejected due to sample processing errors. No CTC were isolated from healthy-volunteers.


CONCLUSIONS
No-differences between daytime and night-time CTC were observed. Therefore, we could not ascertain CTC circadian-rhythm in hospitalized metastasic breast cancer patients.
García-Sáenz
José Angel
JA
Servicio de Oncología Médica. Hospital Universitario San Carlos. Madrid. Spain. jagsaenz@yahoo.com
Martín
Miguel
M
Maestro
Marisa
M
Vidaurreta
Marta
M
Veganzones
Silvia
S
Villalobos
Laura
L
Rodríguez-Lajusticia
Laura
L
Rafael
Sara
S
Sanz-Casla
María Teresa
MT
Casado
Antonio
A
Sastre
Javier
J
Arroyo
Manuel
M
Díaz-Rubio
Eduardo
E
D000970:Antineoplastic Agents / Q000627:therapeutic use; D018931:Antineoplastic Agents, Hormonal / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000097:blood* / Q000188:drug therapy / Q000473:pathology; D002452:Cell Count; D002453:Cell Cycle; D002940:Circadian Rhythm; D020847:Estrogen Receptor Modulators / Q000627:therapeutic use; D004967:Estrogens; D005260:Female; D006801:Humans; D009362:Neoplasm Metastasis / Q000473:pathology*; D009376:Neoplasms, Hormone-Dependent / Q000097:blood / Q000188:drug therapy / Q000473:pathology; D009360:Neoplastic Cells, Circulating; D011446:Prospective Studies
D000970:Antineoplastic Agents; D018931:Antineoplastic Agents, Hormonal; D020847:Estrogen Receptor Modulators; D004967:Estrogens