Chondrogenesis of human periosteum-derived progenitor cells in atelocollagen.
Periosteum-derived progenitor cells (PDPCs) could be differentiated into cartilage using atelocollagen as a carrier and in the presence of transforming growth factor-beta3 (TGF-beta3). Chondrogenesis was verified by RT-PCR and Western blotting. Expression of the type II collagen mRNA was found from the differentiated PDPCs in atelocollagen 3 weeks after chondrogenic induction. The chondrogenic potential of the PDPCs was also verified by histochemical staining for type II collagen protein. Increased production of glycosaminoglycan shows that the PDPCs in atelocollagen could differentiate into chondrocytes under a chondrogenic environment. PDPCs can therefore be used as a cell source for cell-based therapies targeted toward the articular cartilage of the knee.
Choi
Yong-Soo
YS
Department of Biological Engineering, Inha University, Incheon, 402-751, Korea, kimdi@inha.ac.kr.
Lim
Sang-Min
SM
Shin
Hyun-Chong
HC
Lee
Chang-Woo
CW
Kim
Sang-Lin
SL
Kim
Dong-Il
DI
D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D020219:Chondrogenesis / Q000187:drug effects / Q000502:physiology*; D003094:Collagen / Q000378:metabolism*; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D007120:Immunochemistry; D008875:Middle Aged; D010521:Periosteum / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology
D024043:Collagen Type II; D006025:Glycosaminoglycans; D012333:RNA, Messenger; D053782:Transforming Growth Factor beta3; C059821:atelocollagen; D003094:Collagen