title: RNASEN regulates cell proliferation and affects survival in esophageal cancer patients.
abstract: OBJECTIVE
  MicroRNAs (miRNA) are small noncoding RNAs thought to be involved in physiologic and developmental processes by negatively regulating the expression of target genes. Little is known about the role of miRNAs in normal and cancer cells. It is possible that deregulation of miRNA may contribute to the oncogenesis of some cancers. We studied the expression level of the miRNA processing enzyme (DICER1, DGCR8, and RNASEN) in esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  The expression levels of DICER1, DGCR8, and RNASEN mRNA in 73 ESCC tissues were compared with that in corresponding normal esophageal epithelium by Taqman real-time reverse-transcription PCR. We also examined RNASEN protein expression in 27 cell lines. The role of RNASEN in cell proliferation in ESCC cells was assessed by small interfering RNA. Paraffin sections of ESCC patients were immunohistochemically investigated.
  
  
  RESULTS
  We found that RNASEN expression levels were enhanced in a fraction of esophageal cancers. Multivariate Cox regression analysis showed that the prognostic effect of RNASEN (P = 0.0036) seems to be independent of disease stage (P = 0.0060). Knockdown of RNASEN in esophageal cancer cell lines resulted in a 46% to 85% reduction in cell number. In an immunohistochemical study, the intensity of RNASEN expression was often increased in the tumor compared with that in normal epithelium.
  
  
  CONCLUSIONS
  The relationship between the RNASEN expression and the prognosis of the ESCC patients warrants a further study on the role of miRNA and tumor progression.
authors.affiliation: Department of Surgery II, Nagoya City University Medical School, Nagoya, Japan.
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Sugito
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ishiguro
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kuwabara
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kimura
authors.forename: Akira
authors.initials: A
authors.lastname: Mitsui
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kurehara
authors.forename: Takuya
authors.initials: T
authors.lastname: Ando
authors.forename: Ryota
authors.initials: R
authors.lastname: Mori
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Takashima
authors.forename: Ryo
authors.initials: R
authors.lastname: Ogawa
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Fujii
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000401:mortality* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D053487:DEAD-box RNA Helicases / Q000378:metabolism; D004722:Endoribonucleases / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000401:mortality* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D052138:Genes, Neoplasm / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis; D011379:Prognosis; D011506:Proteins / Q000378:metabolism; D016601:RNA-Binding Proteins; D043244:Ribonuclease III; D012260:Ribonucleases / Q000378:metabolism / Q000502:physiology*; D015996:Survival Rate
chemical_list: C474043:DGCR8 protein, human; D009363:Neoplasm Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; D004722:Endoribonucleases; D012260:Ribonucleases; C408087:DICER1 protein, human; C517918:DROSHA protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases