Microglial cell line established from prion protein-overexpressing mice is susceptible to various murine prion strains.
Several lines of evidence suggest that microglia have important roles in the pathogenesis of prion diseases. Here, we establish a novel microglial cell line (MG20) from neonatal tga20 mice that overexpress murine prion protein. After exposure to Chandler scrapie, we observed the replication and accumulation of disease-associated forms of the prion protein in MG20 cells up to the 15th passage. Furthermore, MG20 cells were susceptible to ME7, Obihiro scrapie, and bovine spongiform encephalopathy agents. Thus, MG20 cell lines persistently infected with various murine prion strains provide a useful model for the study of the pathogenesis of prion diseases.
Iwamaru
Yoshifumi
Y
Research Center for Prion Diseases, National Institute of Animal Health, Kannondai 3-1-5, Tsukuba, Ibaraki 305-8602, Japan.
Takenouchi
Takato
T
Ogihara
Kazumasa
K
Hoshino
Megumi
M
Takata
Masuhiro
M
Imamura
Morikazu
M
Tagawa
Yuichi
Y
Hayashi-Kato
Hiroko
H
Ushiki-Kaku
Yuko
Y
Shimizu
Yoshihisa
Y
Okada
Hiroyuki
H
Shinagawa
Morikazu
M
Kitani
Hiroshi
H
Yokoyama
Takashi
T
D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D051379:Mice; D017628:Microglia / Q000166:cytology* / Q000378:metabolism; D018620:PrPSc Proteins / Q000235:genetics / Q000378:metabolism*; D011328:Prions; D012608:Scrapie / Q000473:pathology*
D018620:PrPSc Proteins; D011328:Prions