title: TNT transformation products are affected by the growth conditions of Raoultella terrigena.
abstract: High concentrations of 2,4,6-trinitrotoluene (TNT) and related nitroaromatic compounds are commonly found in soil and groundwater at former explosive plants. The bacterium, Raoultella terrigena strain HB, isolated from a contaminated site, converts TNT into the corresponding amino products. Radio-HPLC analysis with [(14)C]TNT identified aminodinitrotoluene, diaminonitrotoluene and azoxy-dimers as the main metabolites. Transformation rate and the type of metabolites that predominated in the culture medium and within the cells were significantly influenced by the culture conditions. The NAD(P)H-dependent enzymatic reduction of nitro-substituted compounds by cell-free extracts of R. terrigena was evaluated in vitro.
authors.affiliation: Johannes Gutenberg-University Mainz, Institute of Microbiology and Wine Research, Mainz, Germany. hclaus@uni-mainz.de
authors.forename: Harald
authors.initials: H
authors.lastname: Claus
authors.forename: Nina
authors.initials: N
authors.lastname: Perret
authors.forename: Tobias
authors.initials: T
authors.lastname: Bausinger
authors.forename: Gregor
authors.initials: G
authors.lastname: Fels
authors.forename: Johannes
authors.initials: J
authors.lastname: Preuss
authors.forename: Helmut
authors.initials: H
authors.lastname: König
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D004755:Enterobacteriaceae / Q000254:growth & development* / Q000378:metabolism*; D014303:Trinitrotoluene / Q000378:metabolism*
chemical_list: D014303:Trinitrotoluene