Yersinia enterocolitica isolates of differing biotypes from humans and animals are adherent, invasive and persist in macrophages, but differ in cytokine secretion profiles in vitro.
Previous epidemiological studies have demonstrated a potential link between the serotypes of Yersinia enterocolitica recovered from cattle, sheep and pigs and those isolated from human disease cases. Further studies utilizing amplified fragment length polymorphisms have shown a relationship at the genetic level between strains of biotypes 3 and 4 from humans and livestock, and also suggested that some biotype 1A isolates, classically defined as non-pathogenic, are closely related to biotype 3 and 4 isolates. This study sought to understand further the pathogenic potential of Y. enterocolitica isolates from livestock in Great Britain. A range of surrogate in vitro models, such as invasion of epithelial tissue cultures, survival in cultured macrophages and cytokine secretion response, was employed to assess the pathogenicity of 88 strains. The results suggested that all isolates examined were capable of adhering to and invading epithelial cells and of surviving within macrophages. However, the inflammatory response of the infected macrophages differed with the infecting Y. enterocolitica subtype, with the response to pathogenic biotype 3 and 4 isolates different to that observed with biotype 1A isolates, and with the biotype 3 O : 5,27 isolates recovered exclusively from animals. Infections of porcine tissue also suggested the possibility of host-tissue tropism within Y. enterocolitica subtypes.
McNally
Alan
A
Veterinary Laboratories Agency, Woodham Lane, New Haw, Surrey KT15 3NB, UK.
Dalton
Tracey
T
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E, UK.
Ragione
Roberto M La
RM
Veterinary Laboratories Agency, Woodham Lane, New Haw, Surrey KT15 3NB, UK.
Stapleton
Kenneth
K
Veterinary Laboratories Agency, Woodham Lane, New Haw, Surrey KT15 3NB, UK.
Manning
Georgina
G
Veterinary Laboratories Agency, Woodham Lane, New Haw, Surrey KT15 3NB, UK.
Newell
Diane G
DG
Veterinary Laboratories Agency, Woodham Lane, New Haw, Surrey KT15 3NB, UK.
D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D006113:United Kingdom; D015009:Yersinia Infections / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000254:growth & development / Q000276:immunology / Q000502:physiology*
D016207:Cytokines