title: The effect of two different doses comprising the simultaneous administration of intravenous B-complex vitamins and oral folic acid on serum homocysteine levels in hemodialysis patients.
abstract: BACKGROUND
  Several regimens using different doses of folic acid (FA) alone or supplemented with B-complex vitamins (BCVs) have been tested for their ability to reduce total homocysteine (tHcy) serum levels in hemodialysis (HD) patients. In the present study, we assessed the effect of two different doses comprising the simultaneous administration of intravenous (IV) BCVs and an oral FA supplementation on serum tHCy levels in HD patients.
  
  
  METHODS
  In a cohort of 49 patients (31 male, 18 female) undergoing chronic HD treatment for a mean of 40.0+/-40.7 months, serum concentrations of tHcy, folate and vitamin-B12 (vB12) were determined at the end of three sequential periods as follows: 20 weeks without any BCV and/or FA supplementation (period A), 20 weeks with a dose comprising the simultaneous administration of IV BCVs and an oral supplementation of 5 mg of FA once a week (period B), and 20 weeks with a dose comprising the simultaneous administration of IV BCVs and an oral supplementation of 5 mg of FA thrice a week (period C). An IV dose of BCVs consisting of a 5 mL solution containing vitamin B1 (250 mg), vitamin B6 (250 mg) and vitamin B12 (1.5 mg) was administered at the end of hemodialysis.
  
  
  RESULTS
  Mean serum tHcy levels were significantly higher at the end of period A relative to levels at the end of periods B and C (35.8+/-23 micromol/L vs. 22.0+/-17.6 and 15.0+/-4.5 micromol/L, respectively; p<0.000001). Mean serum folate levels and mean serum vB12 levels were significantly lower at the end of period A relative to levels at the end of periods B and C (p<0.000001). Mean serum tHcy levels were lowest at the end of period C (p<0.000001 in comparison to periods A and B), and 26 of the 49 HD patients (67.3%) possessed tHcy levels below 16 micromol/L.
  
  
  CONCLUSIONS
  In HD patients, high doses consisting of the simultaneous administration of IV BCVs and an oral FA supplementation resulted in the efficient reduction of serum tHcy levels.
authors.affiliation: Renal Unit, Biochemical Laboratory, G. Papanikolaou General Hospital, Thessaloniki, Greece. sobolis@hol.gr
authors.forename: Kostas
authors.initials: K
authors.lastname: Sombolos
authors.forename: Anna
authors.initials: A
authors.lastname: Papaioannou
authors.forename: Fotini
authors.initials: F
authors.lastname: Christidou
authors.forename: Taisir
authors.initials: T
authors.lastname: Natse
authors.forename: Gerasimos
authors.initials: G
authors.lastname: Bamichas
authors.forename: Lazaros
authors.initials: L
authors.lastname: Gionanlis
authors.forename: George
authors.initials: G
authors.lastname: Katsaris
authors.forename: Evagelia
authors.initials: E
authors.lastname: Progia
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage*; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D014803:Vitamin B Complex / Q000008:administration & dosage*
chemical_list: D006710:Homocysteine; D014803:Vitamin B Complex; D005492:Folic Acid