Comparative DNA analysis of two vanA plasmids from Enterococcus faecium strains isolated from poultry and a poultry farmer in Norway.
The DNA sequences of two plasmids carrying vanA, pVEF1 (39,626 bp) and pVEF2 (39,714 bp), were determined. Forty-three shared coding sequences were identified, and the only nucleotide difference was an 88-bp indel. A postsegregational killing system was identified. This system possibly explains the persistence of the vanA gene cluster in Norwegian poultry farms.
Sletvold
H
H
Department of Pharmacy, Faculty of Medicine, University of Tromsø, and Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway. hege.sletvold@farmasi.uit.no
Johnsen
P J
PJ
Simonsen
G S
GS
Aasnaes
B
B
Sundsfjord
A
A
Nielsen
K M
KM
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D016984:Enterococcus faecium / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010957:Plasmids; D011200:Poultry / Q000382:microbiology*; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C071437:VanA ligase, Bacteria; D019729:Carbon-Oxygen Ligases