title: Identification and expression of natriuretic peptide receptor type-A and -B mRNA in freshwater and seawater rainbow trout.
abstract: Natriuretic peptide receptors mediate the physiological response of natriuretic peptide hormones. One of the natriuretic peptide receptor types is the particulate guanylyl cyclase receptors, of which there are two identified: NPR-A and NPR-B. In fishes, these have been sequenced and characterized in eels, medaka, and dogfish shark (NPR-B only). The euryhaline rainbow trout provides an opportunity to further pursue examination of the system in teleosts. In this study, partial rainbow trout NPR-A-like and NPR-B-like mRNA sequences were identified via PCR and cloning. The sequence information was used in real-time PCR to examine mRNA expression in a variety of tissues of freshwater rainbow trout and rainbow trout acclimated to 35 parts per thousand seawater for a period of 10 days. In the excretory kidney and posterior intestine, real-time PCR analysis showed greater expression of NPR-B in freshwater fish than in those adapted to seawater; otherwise, there was no difference in the expression of the individual receptors in fresh water or seawater. In general, the expression of the NPR-A and NPR-B type receptors was quite low. These findings indicate that NPR-A and NPR-B mRNA expression is minimally altered under the experimental regime used in this study.
authors.affiliation: Faculty of Science and Technology, School of Life and Environmental Sciences, Deakin University, Pigdons Road, Waurn Ponds, 3217, Geelong, Australia.
authors.forename: Scott
authors.initials: S
authors.lastname: Nankervis
authors.forename: Mark
authors.initials: M
authors.lastname: Powell
authors.forename: Janet
authors.initials: J
authors.lastname: McLeod
authors.forename: Tes
authors.initials: T
authors.lastname: Toop
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D005618:Fresh Water; D005786:Gene Expression Regulation; D006162:Guanylate Cyclase / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D017461:Receptors, Atrial Natriuretic Factor / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D012623:Seawater; D014882:Water-Electrolyte Balance / Q000235:genetics / Q000502:physiology; D000099242:Atrial Natriuretic Factor Receptor B
chemical_list: D006162:Guanylate Cyclase; D012333:RNA, Messenger; D017461:Receptors, Atrial Natriuretic Factor; D000099242:Atrial Natriuretic Factor Receptor B; C091715:atrial natriuretic factor receptor A