Evaluation of the role of H pylori infection in pathogenesis of gastric cancer by immunoblot assay.
OBJECTIVE
To elucidate the different serological reactions to H pylori using the immunoblotting technique for further understanding of its pathogenic role in gastric cancer.


METHODS
A total of 54 patients were divided into two groups after upper gastrointestinal endoscopy: normal control group (25 patients) and gastric cancer group (29 patients). Both groups were further divided into H pylori (+) and H pylori (-) subgroups based on the results of CLO test, Giemsa staining and culture. Sera were further analyzed with the immunoblotting technique (HelicoBlot 2.0, Genelabs Diagnostics, Singapore).


RESULTS
The positive rate of the immunoblotting test was as high as 88.9% in the H pylori (-) gastric cancer group and only 14.3% in the H pylori (-) normal control group with a statistically significant difference.


CONCLUSIONS
The prevalence of H pylori infection is higher in gastric cancer patients than in the normal controls, suggesting that H pylori may play a role in the pathogenesis of gastric cancer.
Yang
Kuo-Ching
KC
Division of Gastroenterology, Department of Internal Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, China.
Chu
Alexander
A
Liao
Chao-Sheng
CS
Lin
Yu-Min
YM
Wang
Gen-Min
GM
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000276:immunology; D015153:Blotting, Western / Q000379:methods*; D016022:Case-Control Studies; D005260:Female; D016481:Helicobacter Infections / Q000150:complications* / Q000175:diagnosis / Q000453:epidemiology; D016480:Helicobacter pylori / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000382:microbiology*
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G