In vivo sampling with solid phase microextraction.
This review discusses the most recent developments and future challenges in the application of solid phase microextraction (SPME) for sampling of live biological samples. The emphasis is placed on applications of fiber SPME for analysis of volatile emissions and drugs in biological fluids. The method development section highlights the main parameters that need to be considered in the case of in vivo experiments: extraction techniques, selection of extraction phases, calibration procedures, determination of free concentrations, and automation.
Musteata
Florin Marcel
FM
Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada.
Pawliszyn
Janusz
J
D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D009930:Organic Chemicals / Q000032:analysis*; D012867:Skin / Q000378:metabolism*; D052616:Solid Phase Extraction / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D014835:Volatilization
D009930:Organic Chemicals