title: Effect of drug-induced ascorbic acid release in the striatum and the nucleus accumbens in hippocampus-lesioned rats.
abstract: The mechanism of ethanol, morphine, methamphetamine (MAP), and nicotine-induced ascorbic acid (AA) release in striatum, and nucleus accumbens (NAc) is not well understood. Our previous study showed that the glutamatergic system was involved in the addictive drug-induced AA release in NAc and striatum. Furthermore, frontal decortication eliminates drug-induced ascorbic acid release in the striatum but not in the NAc. In the present study, the roles of the hippocampus in drug-induced AA release in the striatum and NAc were studied by using microdialysis coupled to high performance liquid chromatography with electrochemical detection (HPLC-ECD). Ethanol (3.0 g/kg, i.p.), methamphetamine (3.0 mg/kg, i.p.), and nicotine (1.5 mg/kg, i.p.) significantly stimulated AA release in the striatum and NAc, respectively. Morphine (20 mg/kg, i.p.) significantly stimulated AA release in the striatum, but not in the NAc. After hippocampal lesion by kainic acid, AA release induced by ethanol, methamphetamine, and nicotine could be eliminated in NAc, but not in the striatum. These results suggest that the hippocampus might be a common and necessary area in addictive drug-induced AA release in the NAc, which also imply that different pathways might be involved in drug-induced AA release in the striatum and the NAc of the rats.
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Dai
authors.forename: Jing Yu
authors.initials: JY
authors.lastname: Yang
authors.forename: Pei Fei
authors.initials: PF
authors.lastname: Gu
authors.forename: Yue
authors.initials: Y
authors.lastname: Hou
authors.forename: Chun Fu
authors.initials: CF
authors.lastname: Wu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D001923:Brain Chemistry / Q000187:drug effects; D001930:Brain Injuries / Q000139:chemically induced / Q000473:pathology; D002492:Central Nervous System Depressants / Q000494:pharmacology; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology; D006624:Hippocampus / Q000293:injuries / Q000473:pathology / Q000503:physiopathology*; D007395:Interneurons / Q000502:physiology; D007608:Kainic Acid; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology; D017551:Microdialysis / Q000379:methods; D009020:Morphine / Q000494:pharmacology; D009294:Narcotics / Q000494:pharmacology; D009538:Nicotine / Q000494:pharmacology; D018722:Nicotinic Agonists / Q000494:pharmacology; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002492:Central Nervous System Depressants; D000697:Central Nervous System Stimulants; D009294:Narcotics; D018722:Nicotinic Agonists; D000431:Ethanol; D008694:Methamphetamine; D009538:Nicotine; D009020:Morphine; D001205:Ascorbic Acid; D007608:Kainic Acid