title: Identification of proteoglycan-binding proteins.
abstract: There are many proteins that bind to proteoglycans; they include proteins in extracellular matrices, growth factors or cytokines, plasma proteins, transmembrane proteins, and cytoplasmic proteins as listed in this chapter. Proteins that bind to a proteoglycan have been searched by using a proteoglycan as a ligand. Alternatively, a ligand protein has been used to find a proteoglycan as a binding partner. When the glycosaminoglycan (GAG) portion of a proteoglycan is responsible for the binding, a native proteoglycan is necessary for the analysis of binding. When the protein portion is responsible for the binding, a recombinant core protein without GAG chains may be used for analysis. This chapter describes experimental procedures dealing with two native proteoglycans, versican (PG-M) and syndecan-4 (ryudocan). Versican has been identified as a proteoglycan with binding capability to a growth factor, midkine. Purified syndecan-4 has been used to identify proteins that bind to the proteoglycan.
authors.affiliation: Department of Health Science, Faculty of Psychological and Physical Sciences, Aichi Gakuin University, Aichi, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Muramatsu
authors.forename: Hisako
authors.initials: H
authors.lastname: Muramatsu
authors.forename: Tetsuhito
authors.initials: T
authors.lastname: Kojima
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008024:Ligands; D051379:Mice; D000078792:Midkine; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D053671:Syndecan-4 / Q000737:chemistry* / Q000378:metabolism; D053675:Versicans / Q000737:chemistry* / Q000378:metabolism
chemical_list: D016207:Cytokines; D008024:Ligands; D011506:Proteins; D053671:Syndecan-4; D053675:Versicans; D000078792:Midkine