title: Effect of Agave tequilana juice on cell wall polysaccharides of three Saccharomyces cerevisiae strains from different origins.
abstract: In this study, a characterization of cell wall polysaccharide composition of three yeasts involved in the production of agave distilled beverages was performed. The three yeast strains were isolated from different media (tequila, mezcal and bakery) and were evaluated for the beta(1,3)-glucanase lytic activity and the beta-glucan/ mannan ratio during the fermentation of Agave tequilana juice and in YPD media (control). Fermentations were performed in shake flasks with 30 g l(-1) sugar concentration of A. tequilana juice and with the control YPD using 30 g l(-1) of glucose. The three yeasts strains showed different levels of beta-glucan and mannan when they were grown in A. tequilana juice in comparison to the YPD media. The maximum rate of cell wall lyses was 50% lower in fermentations with A. tequilana juice for yeasts isolated from tequila and mezcal than compared to the bakery yeast.
authors.affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Normalistas 800 Col. Colinas de la Normal, Guadalajara, Jalisco C.P. 44270, México. blancaaguilaru@hotmail.com
authors.forename: Blanca
authors.initials: B
authors.lastname: Aguilar-Uscanga
authors.forename: Javier
authors.initials: J
authors.lastname: Arrizon
authors.forename: Jesús
authors.initials: J
authors.lastname: Ramirez
authors.forename: Josué
authors.initials: J
authors.lastname: Solis-Pacheco
mesh_terms: D027882:Agave / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D005285:Fermentation; D005516:Food Microbiology; D043326:Glucan 1,3-beta-Glucosidase / Q000032:analysis; D008351:Mannans / Q000032:analysis; D010936:Plant Extracts / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D047071:beta-Glucans / Q000032:analysis
chemical_list: D008351:Mannans; D010936:Plant Extracts; D011134:Polysaccharides; D047071:beta-Glucans; D043326:Glucan 1,3-beta-Glucosidase; C117648:beta-1,3-exoglucanase