title: Determination of 6-gingerol in ginger (Zingiber officinale) using high-performance thin-layer chromatography.
abstract: A sensitive and accurate High-Performance TLC (HPTLC) method has been developed to determine the quantity of 6-gingerol in rhizomes of Zingiber officinale (family: Zingiberaceae), commonly known as ginger. Methanol extracts of rhizomes from three different sources were used for HPTLC, n-hexane, and diethyl ether (40:60 v/v) as the mobile phase. The Rf of 6-gingerol was found to be 0.40. The calibration plot was linear in the range of 250-1200 ng of 6-gingerol and the correlation coefficient of 0.9997 was indicative of good linear dependence of peak area on concentration. The mean quantity of 6-gingerol was found to be 60.44+/-2.53 mg/g of ginger extract. The method permits reliable quantification of 6-gingerol and good resolution and separation of 6-gingerol from other constituents of ginger. To study the accuracy and precision of the method, recovery studies were performed by the method of standard addition. Recovery values from 99.79 to 99.84% showed the excellent reliability and reproducibility of the method. The proposed HPTLC method for quantitative monitoring of 6-gingerol in ginger can be used for routine quality testing of ginger extracts.
authors.affiliation: School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.
authors.forename: Sujay
authors.initials: S
authors.lastname: Rai
authors.forename: Kakali
authors.initials: K
authors.lastname: Mukherjee
authors.forename: Mainak
authors.initials: M
authors.lastname: Mal
authors.forename: Atul
authors.initials: A
authors.lastname: Wahile
authors.forename: Bishnu Pada
authors.initials: BP
authors.lastname: Saha
authors.forename: Pulok K
authors.initials: PK
authors.lastname: Mukherjee
mesh_terms: D002396:Catechols; D002855:Chromatography, Thin Layer / Q000379:methods* / Q000706:statistics & numerical data; D005233:Fatty Alcohols / Q000032:analysis*; D005504:Food Analysis / Q000379:methods / Q000706:statistics & numerical data; D020939:Zingiber officinale / Q000737:chemistry*
chemical_list: D002396:Catechols; D005233:Fatty Alcohols; C007845:gingerol