title: The crude venom from the sea anemone Stichodactyla helianthus induces haemolysis and slight peroxidative damage in rat and human erythrocytes.
abstract: The haemolytic and peroxidative effects of crude venom of the sea anemone Stichodactyla helianthus were evaluated in rat and human erythrocytes. Venom extract caused a significant concentration-dependent effect on haemolysis (release of haemoglobin). Human erythrocytes were more sensitive (0.094 mg protein/ml) than those of the rats (0.3787 mg protein/ml). In contrast, a light effect on lipid peroxidation (LP, an index of oxidative damage to membrane lipids) was recorded. The concentrations needed to produce a significant effect on LP in rat and human erythrocytes were, respectively, 2-fold and 7-fold higher than those required to produce significant haemolysis. The differential effect of S. helianthus venom on haemolysis and oxidation of membrane lipids is not common for venoms of other sea anemones, which usually show a tightly related effect on LP and haemolytic damage.
authors.affiliation: Universidad Nacional Autónoma de México, Instituto de Ciencias del Mar y Limnología, Unidad Académica Puerto Morelos, 77500, Cancún, Quintana Roo, México.
authors.forename: Heidi Irais
authors.initials: HI
authors.lastname: Monroy-Estrada
authors.forename: Lourdes
authors.initials: L
authors.lastname: Segura-Puertas
authors.forename: Sonia
authors.initials: S
authors.lastname: Galván-Arzate
authors.forename: Abel
authors.initials: A
authors.lastname: Santamaría
authors.forename: Judith
authors.initials: J
authors.lastname: Sánchez-Rodríguez
mesh_terms: D000818:Animals; D003064:Cnidarian Venoms / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D011042:Poisons / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012615:Sea Anemones / Q000737:chemistry* / Q000378:metabolism; D013045:Species Specificity; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D003064:Cnidarian Venoms; D011042:Poisons; D017392:Thiobarbituric Acid Reactive Substances