Sequence analysis of the nucleoprotein gene of Asian lineage peste des petits ruminants vaccine virus.
The complete nucleotide sequence of the nucleocapsid (N) protein of the peste-des-petits ruminants vaccine virus (PPRV Sungri/96) belonging to the Asian lineage was determined. The gene was 1692 nucleotides in length and encoded a polypeptide of 525 amino acids. The PPRV Sungri/96 N gene has a nucleotide homology of 92% for PPRV Nigeria 75/1 to 55.5% for canine distemper virus. At amino acid level the homology was 94.1% with PPRV Nigeria 75/1, while with other morbilliviruses, PPRV Sungri/96 had only 71.4-64.9% amino acid identity. The phosphorylation prediction reveals eight conserved sites across morbilliviruses, whereas in the C-terminal portion of the protein the sites are not conserved. Phylogenetic analysis of different N proteins of morbilliviruses revealed five well-defined clusters as observed previously. To the best of our knowledge this is the first report describing the nucleocapsid gene sequence of PPRV Indian isolate.
Muthuchelvan
D
D
Central Institute of Fisheries Technology, Cochin, India.
Sanyal
A
A
Balamurugan
V
V
Dhar
P
P
Bandyopadhyay
S K
SK
D000595:Amino Acid Sequence; D000818:Animals; D001208:Asia; D001483:Base Sequence; D002522:Chlorocebus aethiops; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000235:genetics*; D029021:Peste-des-Petits-Ruminants / Q000821:virology*; D018107:Peste-des-petits-ruminants virus / Q000235:genetics*; D010802:Phylogeny; D014709:Vero Cells; D014765:Viral Vaccines / Q000235:genetics*
D009698:Nucleoproteins; D014765:Viral Vaccines