Challenges related to analysis of protein spot volumes from two-dimensional gel electrophoresis as revealed by replicate gels.
Assumptions that need to be considered prior to statistical analysis of protein spot volumes from two-dimensional gel electrophoresis (2-DE) data are studied using replicate gels of the same sample. The most important observation is that the data tables of protein spot volumes from 2-DE images contain a large number of missing values, which are not consistent with the presence or absence of the proteins. This implies both loss of information and problems for the subsequent statistical analysis. Challenges with 2-DE protein spot volumes are viewed in light of multiple gel comparisons and multivariate data analysis.
Grove
Harald
H
Matforsk AS, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway.
Hollung
Kristin
K
Uhlen
Anne Kjersti
AK
Martens
Harald
H
Faergestad
Ellen Mosleth
EM
D003627:Data Interpretation, Statistical; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D015999:Multivariate Analysis; D011506:Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D014908:Triticum / Q000235:genetics
D011506:Proteins