title: Development of a harmonised method for the profiling of amphetamines: IV. Optimisation of sample preparation.
abstract: The suitability of liquid-liquid extraction (LLE) and solid-phase extraction (SPE) for the preparation of impurity extracts intended for gas chromatographic profiling analyses of amphetamine were evaluated. Both techniques were optimised with respect to the extraction of selected target compounds by use of full factorial designs in which the variables affecting the performance were evaluated. Test samples consisted of amphetamine synthesised by the Leuckart reaction, by reductive amination of benzyl methyl ketone and by the nitrostyrene route. The performance of LLE and SPE were comparable in terms of repeatability and recovery of the target compounds. LLE was considered the better choice for the present harmonised amphetamine profiling method due to the lack of information on the long-term stability of SPE columns.
authors.affiliation: Swedish National Laboratory of Forensic Science-SKL, SE-581 94 Linköping, Sweden. kjell-b.andersson@skl.polisen.se
authors.forename: Kjell
authors.initials: K
authors.lastname: Andersson
authors.forename: Kaisa
authors.initials: K
authors.lastname: Jalava
authors.forename: Eric
authors.initials: E
authors.lastname: Lock
authors.forename: Henk
authors.initials: H
authors.lastname: Huizer
authors.forename: Elisabet
authors.initials: E
authors.lastname: Kaa
authors.forename: Alvaro
authors.initials: A
authors.lastname: Lopes
authors.forename: Anneke
authors.initials: A
authors.lastname: Poortman-van der Meer
authors.forename: Michael D
authors.initials: MD
authors.lastname: Cole
authors.forename: Johan
authors.initials: J
authors.lastname: Dahlén
authors.forename: Erkki
authors.initials: E
authors.lastname: Sippola