Glycodelin mediates the increase in vascular endothelial growth factor in response to oxidative stress in the endometrium.
OBJECTIVE
We hypothesized that glycodelin stimulates vascular endothelial growth factor (VEGF) expression in response to oxidative stress.


METHODS
EM42 (human endometrial epithelial cell line) and primary endometrial epithelial cells were subjected to oxidative stress with minimally oxidized low density lipoprotein (mLDL). Cells were also incubated with no LDL (control) or native LDL (nLDL). Each condition was incubated with and without glycodelin antibody. Glycodelin and VEGF protein and messenger RNA (mRNA) levels were analyzed. Primary cells were cultured with glycodelin peptide to evaluate the effect on VEGF protein and mRNA.


RESULTS
Glycodelin and VEGF protein and mRNA were higher for cells grown with mLDL (P < .05), while glycodelin antibody attenuated the increase in VEGF protein (P < .01). Glycodelin peptide increased VEGF mRNA and protein (P < .05).


CONCLUSIONS
Glycodelin may act as an autocrine factor within endometriotic implants to increase VEGF expression during oxidative stress.
Park
John K
JK
Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA, USA.
Song
MingQing
M
Dominguez
Celia E
CE
Walter
Mary F
MF
Santanam
Nalini
N
Parthasarathy
Sampath
S
Murphy
Ana A
AA
D000906:Antibodies / Q000494:pharmacology; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology; D018384:Oxidative Stress / Q000502:physiology*; D011257:Pregnancy Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
D000906:Antibodies; D000071816:Glycodelin; D006023:Glycoproteins; D008077:Lipoproteins, LDL; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C099252:oxidized low density lipoprotein