Tyrosine 110 in the measles virus phosphoprotein is required to block STAT1 phosphorylation.
The measles virus (MV) P gene encodes three proteins: P, an essential polymerase cofactor, and C and V, which have multiple functions including immune evasion. We show here that the MV P protein also contributes to immune evasion, and that tyrosine 110 is required to block nuclear translocation of the signal transducer and activator of transcription factors (STAT) after interferon type I treatment. In particular, MV P inhibits STAT1 phosphorylation. This is shown not only by transient expression but also by reverse genetic analyses based on a new functional infectious cDNA derived from a MV vaccine vial (Moraten strain). Our study also identifies a conserved sequence around P protein tyrosine 110 as a candidate interaction site with a cellular protein.
Devaux
Patricia
P
Molecular Medicine Program and Virology and Gene Therapy Graduate Track, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
von Messling
Veronika
V
Songsungthong
Warangkhana
W
Springfeld
Christoph
C
Cattaneo
Roberto
R
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D006367:HeLa Cells; D006801:Humans; D007113:Immunity, Innate; D007370:Interferon Type I / Q000494:pharmacology; D008457:Measles / Q000276:immunology / Q000821:virology*; D008459:Measles virus / Q000378:metabolism*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D050794:STAT1 Transcription Factor / Q000378:metabolism*; D016415:Sequence Alignment; D015398:Signal Transduction; D014443:Tyrosine / Q000502:physiology*; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D007370:Interferon Type I; C055859:P protein, Sendai virus; D010750:Phosphoproteins; D050794:STAT1 Transcription Factor; C069181:V protein, measles virus; D014764:Viral Proteins; D014443:Tyrosine