Effects of sonicated Prevotella intermedia, Fusobacterium nucleatum and Lactobacillus casei extracts on interleukin-8 production by human dental pulp cells.
The objective of this study was to determine the effects of Prevotella intermedia, Fusobacterium nucleatum and Lactobacillus casei on the production of IL-8 by human dental pulp cells. Human dental pulp cells from teeth of young patients (aged 18-25 years) were cultured and tested with sonicated P. intermedia ATCC 25611, F. nucleatum ATCC 25586 and L. casei ATCC 4646 extracts. IL-8 secreted into the culture supernatants were measured at 6, 12 and 24 hours using a quantitative sandwich enzyme immunoassay technique. Cell viability was evaluated using trypan blue exclusion technique. IL-8 production by human dental pulp cells increased significantly at 12 and 24 hours after exposure to P. intermedia and F. nucleatum, whereas L. casei extract exhibited low IL-8 production. The sonicated bacterial extracts did not significantly affect viability or total number of dental pulp cells.
Thaweboon
Boonyanit
B
Department of Microbiology, Faculty of Dentistry, Mahidol University, 6 Yothi Road, Bangkok 10400, Thailand. boonitdt@yahoo.com
Thaweboon
Sroisiri
S
Choonharuangdej
Suwan
S
Suppakpatana
Prapan
P
D000293:Adolescent; D000328:Adult; D001424:Bacterial Infections / Q000378:metabolism*; D002470:Cell Survival; D002478:Cells, Cultured; D003782:Dental Pulp / Q000378:metabolism / Q000382:microbiology*; D045182:Fusobacteria; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D007780:Lacticaseibacillus casei; D018713:Prevotella intermedia
D016209:Interleukin-8