Bacisubin, an antifungal protein with ribonuclease and hemagglutinating activities from Bacillus subtilis strain B-916.
An antifungal protein, with a molecular mass of 41.9 kDa, and designated as bacisubin, was isolated from a culture of Bacillus subtilis strain B-916. The isolation procedure consisted of ion exchange chromatography on DEAE-Sepharose Fast Flow, and fast protein liquid chromatography on Phenyl Sepharose 6 Fast Flow and hydroxyapatite columns. The protein was adsorbed on all three chromatographic media. Bacisubin exhibited inhibitory activity on mycelial growth in Magnaporthe grisease, Sclerotinia sclerotiorum, Rhizoctonia solani, Alternaria oleracea, A. brassicae and Botrytis cinerea. The IC50 values of its antifungal activity toward the last four fungal species were 4.01 microM, 0.087 microM, 0.055 microM and 2.74 microM, respectively. Bacisubin demonstrated neither protease activity, nor protease inhibitory activity. However, it manifested ribonuclease and hemagglutinating activities.
Liu
Yongfeng
Y
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Chen
Zhiyi
Z
Ng
T B
TB
Zhang
Jie
J
Zhou
Mingguo
M
Song
Fuping
F
Lu
Fan
F
Liu
Youzhou
Y
D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D001412:Bacillus subtilis / Q000737:chemistry* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D006384:Hemagglutination / Q000187:drug effects; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D011817:Rabbits; D012260:Ribonucleases / Q000235:genetics / Q000302:isolation & purification
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; D012260:Ribonucleases