title: Generating vesicular stomatitis virus pseudotype bearing the severe acute respiratory syndrome coronavirus spike envelope glycoprotein for rapid and safe neutralization test or cell-entry assay.
abstract: We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Delta G*SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARS-CoV in trans. The neutralization and infection blocking tests showed that the VSV Delta G*SG and SARS-CoV reacted similarly to SARS-CoV specific antiserum, suggesting the VSVDelta G*SG can be a safe replacement of the live SARS-CoV for neutralization test and cell-entry assay.
authors.affiliation: National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R. China.
authors.forename: Jinying
authors.initials: J
authors.lastname: Ge
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Wen
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
authors.forename: Sen
authors.initials: S
authors.lastname: Hu
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiangang
authors.initials: X
authors.lastname: Kong
authors.forename: Hualan
authors.initials: H
authors.lastname: Chen
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Bu
mesh_terms: D000818:Animals; D002645:Chickens; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D008562:Membrane Glycoproteins / Q000276:immunology*; D009500:Neutralization Tests / Q000592:standards / Q000662:veterinary; D018353:Rhabdoviridae Infections / Q000276:immunology / Q000662:veterinary*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D012449:Safety; D013047:Specific Pathogen-Free Organisms; D064370:Spike Glycoprotein, Coronavirus; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins