A microsatellite linkage map of the blacklip abalone, Haliotis rubra.
There is considerable scope for genetic improvement of cultured blacklip abalone Haliotis rubra in Australia using molecular marker-assisted, selective-breeding practices. Such improvement is dependent on the availability of primary genetic resources, such as a genetic linkage map. This study presents a first-generation linkage map of H. rubra, containing 122 microsatellite markers typed in a single full-sib family. These loci mapped to 17 and 20 linkage groups for the male and female respectively, and when aligned, the consensus map represented 18 linkage groups. The male linkage map contained 102 markers (one unlinked) covering 621 cM with an average intermarker spacing of 7.3 cM, and the female map contained 98 markers (eight unlinked) covering 766 cM with an average intermarker spacing of 9.8 cM. Analysis of markers informative in both parents showed a significantly higher recombination rate in the female parent, with an average male-to-female recombination ratio of 1:1.45 between linked pairs of markers. This linkage map represents a significant advancement in the genetic resource available for H. rubra and provides a framework for future quantitative trait loci mapping and eventual implementation of marker-assisted selection.
Baranski
M
M
AKVAFORSK (Institute for Aquaculture Research AS), As N-1432, Norway. matt.baranski@akvaforsk.no
Loughnan
S
S
Austin
C M
CM
Robinson
N
N
D000818:Animals; D001315:Australia; D002874:Chromosome Mapping; D005260:Female; D049851:Gastropoda / Q000235:genetics*; D016678:Genome; D008297:Male; D018895:Microsatellite Repeats; D011995:Recombination, Genetic; D012737:Sex Factors