Neurite guidance on protein micropatterns generated by a piezoelectric microdispenser.
In this study, we developed a microdispenser technique in order to create protein patterns for guidance of neurites from cultured adult mouse dorsal root ganglia (DRG). The microdispenser is a micromachined silicon device that ejects 100 picolitre droplets and has the ability to position the droplets with a precision of 6-8 microm. Laminin and bovine serum albumin (BSA) was used to create adhesive and non-adhesive protein lines on polystyrene surfaces (cell culture dishes). Whole-mounted DRGs were then positioned close to the patterns and neurite outgrowth was monitored. The neurites preferred to grow on laminin lines as compared to the unpatterned plastic. When patterns were made from BSA the neurites preferred to grow in between the lines on the unpatterned plastic surface. We conclude that microdispensing can be used for guidance of sensory neurites. The advantages of microdispensing is that it is fast, flexible, allows deposition of different protein concentrations and enables patterning on delicate surfaces due to its non-contact mode of operation. It is conceivable that microdispensing can be utilized for the creation of protein patterns for guiding neurites to obtain in vitro neural networks, in tissue engineering or rapid screening for guiding proteins.
Gustavsson
Per
P
Department of Cell and Organism Biology, Lund University, Helgonavägen 3B, SE 223 62, Lund, Sweden. per.gustavsson@cob.lu.se
Johansson
Fredrik
F
Kanje
Martin
M
Wallman
Lars
L
Linsmeier
Cecilia Eriksson
CE
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D016764:Cell Polarity; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology; D007797:Laminin / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D008845:Microinjections / Q000295:instrumentation / Q000379:methods*; D016501:Neurites / Q000502:physiology*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D014732:Vibration
D020099:Coated Materials, Biocompatible; D007797:Laminin; D012710:Serum Albumin, Bovine