title: A peptide with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Russula paludosa.
abstract: Hot water extracts of 16 species of mushrooms, including both edible and medicinal mushrooms, were screened for human immunodeficiency virus (HIV)-1 reverse transcriptase (RT) inhibitory activity. Extracts of Lactarius camphoratus, Trametes suaveolens, Sparassis crispa, Pleurotus sajor-caju, Pleurotus pulmonarius, and Russula paludosa elicited over 50% inhibition when tested at the concentration of 1 mg/ml. The extract of R. paludosa demonstrated the highest inhibitory activity on HIV-1 RT (97.6%). Fraction SU2, purified from R. paludosa extract by anion exchange chromatography on DEAE-cellulose and gel filtration on Superdex 75, exhibited potent inhibitory activity on HIV-1 RT. At the concentrations of 1 mg/ml, 0.2 mg/ml, and 0.04 mg/ml, the inhibition ratios were 99.2%, 89.3%, and 41.8%, respectively, giving an IC50 of 11 microM. The molecular mass of SU2 was 4.5 kDa and its N-terminal amino acid sequence was determined to be KREHGQHCEF. The peptide was devoid of hemagglutinating, ribonuclease, antifungal, protease, protease inhibitory, and laccase activities.
authors.affiliation: Department of Chemistry, Peking University, Beijing 100080, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Wang
authors.forename: H X
authors.initials: HX
authors.lastname: Wang
authors.forename: T B
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D019380:Anti-HIV Agents / Q000302:isolation & purification* / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D018894:Reverse Transcriptase Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D019380:Anti-HIV Agents; D005656:Fungal Proteins; D010455:Peptides; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase