Development and validation of a competitive ELISA kit for the serological diagnosis of ovine, caprine and bovine brucellosis.
A competitive ELISA (Brucella-Ab c-ELISA) was standardized and validated for the detection of Brucella antibodies in cattle, sheep and goat sera using a monoclonal antibody (MAb 4B5A) produced against Brucella melitensis biotype 2. The specificity and sensitivity of the assay were 100% to a 67.5% cut-off point (B/Bo%). When compared with an indirect ELISA, the Brucella-Ab c-ELISA did not demonstrate cross-reactions when testing positive sera for antibodies to some Enterobacteriaceae. A comparison was made between the Brucella-Ab c-ELISA and the complement fixation and Rose Bengal tests. Results demonstrated that the Brucella-Ab c-ELISA is a valuable tool for the serological diagnosis of bovine and ovine/caprine brucellosis.
Portanti
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e, del Molise G. Caporale. Campo Boario, 64100 Teramo, Italy. o.portanti@izs.it
Tittarelli
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Di Febo
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Luciani
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Mercante
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Conte
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Lelli
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D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D002007:Brucellosis, Bovine / Q000097:blood / Q000175:diagnosis*; D002417:Cattle; D003168:Complement Fixation Tests / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary*; D006041:Goats; D011786:Quality Control; D012016:Reference Values; D015203:Reproducibility of Results; D012395:Rose Bengal; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D000907:Antibodies, Bacterial; D012395:Rose Bengal