Preparation of monoclonal antibodies for detection and identification of Francisella tularensis.
Monoclonal antibodies (MAbs) against Francisella tularensis were obtained. Three MAbs specifically reacted with F. tularensis, while four MAbs reacted with other members of the genus Francisella as well. Fluorescent isothiocyanate-conjugated MAbs unequivocally stained bacterial cells in specimens from experimentally infected mice. Two MAbs agglutinated F. tularensis antigen in the agglutination tests. These MAbs should improve methods for detection and identification of F. tularensis.
Hotta
Akitoyo
A
Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Tokyo 162-8640, Japan. ahotta@nih.go.jp
Uda
Akihiko
A
Fujita
Osamu
O
Tanabayashi
Kiyoshi
K
Yamada
Akio
A
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D005604:Francisella tularensis / Q000276:immunology / Q000302:isolation & purification*; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014406:Tularemia / Q000175:diagnosis
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal