Condensation of interphase chromosomes in nuclei of synchronized CHO cells.
The escape of individual interphase chromosomes from nuclei of reversibly permeabilized Chinese hamster ovary (CHO) cells was utilized for the visualization of condensing interphase chromosomes in a cell cycle-dependent manner in synchronized cells. Major interphase chromosomal forms include: (a) mid-S-phase globular chromosomes at 3.0 C-value, (b) late mid-S-phase fibrous hemicircular forms (3.3 C), (c) late-S-phase supercoiled ribbons (3.7 C), and (d) end-S-phase elongated, bent prechromosomal structures (4.0 C).
Banfalvi
Gaspar
G
Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen, Hungary. bgaspar@delfin.klte.hu
D000818:Animals; D016466:CHO Cells; D002467:Cell Nucleus / Q000648:ultrastructure*; D002469:Cell Separation; D033481:Chromosomes, Mammalian / Q000378:metabolism* / Q000648:ultrastructure; D006224:Cricetinae; D005434:Flow Cytometry; D007399:Interphase; D008856:Microscopy, Fluorescence