Donor-reactive cytokine production after HLA-identical living related kidney transplantation: a protein-array analysis.
In the present pilot study, we investigated which proteins are produced after donor stimulation of peripheral blood mononuclear cells from recipients of HLA-identical living related kidney transplant. We used a protein-array analysis to determine cytokines, chemokines, and growth factors in supernatant from donor-stimulated mixed lymphocyte reaction cultures. Autologous cultures were considered to be negative controls. In 38 out of 42 proteins (90%), the donor response was higher compared with the autologous response. Therefore, we concluded that even after HLA-identical living related kidney transplantation we could measure a donor-reactive response, which we assumed was directed toward minor histocompatibility or non-HLA antigens.
Gerrits
J H
JH
Internal Medicine-Transplantation, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands. j.gerrits@erasmusmc.nl
van de Wetering
J
J
Weimar
W
W
van Besouw
N M
NM
D018925:Chemokines / Q000235:genetics; D016207:Cytokines / Q000235:genetics*; D005190:Family; D015433:Glomerulonephritis, Membranous / Q000276:immunology / Q000601:surgery; D006133:Growth Substances / Q000235:genetics; D006650:Histocompatibility Testing; D006801:Humans; D007378:Interleukins / Q000235:genetics; D016030:Kidney Transplantation / Q000276:immunology / Q000502:physiology*; D019520:Living Donors; D010865:Pilot Projects; D040081:Protein Array Analysis
D018925:Chemokines; D016207:Cytokines; D006133:Growth Substances; D007378:Interleukins