title: [Role of MAPK signal transduction pathway phosphorylation induced by granulocyte-macrophage-colony stimulating factor in the proliferation of hormone-independent prostate cancer].
abstract: OBJECTIVE
  To investigate the phosphorylation intensity of MAPK pathway molecular Erk1/2 and the proliferation of prostate cancer cell line PC-3M.
  
  
  METHODS
  Flow cytometry and RT-PCR were employed to study the ratio of different cell cycles and phases, respectively, before and after GM-CSF stimulation. Erk1/2 phosphorylation intensity was examined by Western blot simultaneously.
  
  
  RESULTS
  The rate of PC-3M cells at S and G2/M stages and the expression intensity of Ki-67 increased after GM-CSF incubation in a dose-dependent manner. The phosphorylation intensity of Erk1/2 increased remarkably after stimulation with GM-CSF.
  
  
  CONCLUSIONS
  The intensification of Erk1/2 phosphorylation is one important molecular mechanism of the proliferation of hormone-independent prostate cancer.
authors.affiliation: Institute of Urology, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China. zhaohuichen@hust.edu.cn
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Chen
authors.forename: Hua-Fang
authors.initials: HF
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Ju
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Xiao
authors.forename: Fu-Qing
authors.initials: FQ
authors.lastname: Zeng
authors.forename: Chuan-Guo
authors.initials: CG
authors.lastname: Xiao
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism / Q000502:physiology*; D009376:Neoplasms, Hormone-Dependent / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D010766:Phosphorylation; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D019394:Ki-67 Antigen; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D019950:Mitogen-Activated Protein Kinase 1; D020929:Mitogen-Activated Protein Kinase Kinases