Latent protein LANA2 from Kaposi's sarcoma-associated herpesvirus interacts with 14-3-3 proteins and inhibits FOXO3a transcription factor.
The Kaposi's sarcoma-associated herpesvirus latent protein LANA2 has been suggested to have an important role in the transforming activity of the virus based on its capacity to inhibit p53 and PKR-dependent apoptosis as well as the interferon-dependent response. Here, we describe a novel interaction between LANA2 and both the phosphoserine/phosphothreonine-binding 14-3-3 proteins and the transcription factor FOXO3a. In addition, our results indicate that LANA2 inhibits the transcriptional activity of FOXO3a and blocks the G2/M arrest induced by 14-3-3 protein overexpression. These results suggest a novel mechanism by which LANA2 may promote tumorigenesis.
Muñoz-Fontela
Cesar
C
Microbiologia II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Marcos-Villar
Laura
L
Gallego
Pedro
P
Arroyo
Javier
J
Da Costa
Marco
M
Pomeranz
Karen M
KM
Lam
Eric W-F
EW
Rivas
Carmen
C
D048948:14-3-3 Proteins / Q000378:metabolism*; D000956:Antigens, Viral / Q000494:pharmacology*; D002460:Cell Line; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000037:antagonists & inhibitors*; D019288:Herpesvirus 8, Human / Q000737:chemistry*; D006801:Humans; D009687:Nuclear Proteins / Q000494:pharmacology*; D012514:Sarcoma, Kaposi / Q000503:physiopathology; D017735:Virus Latency / Q000235:genetics / Q000502:physiology
D048948:14-3-3 Proteins; D000956:Antigens, Viral; C499517:FOXO3 protein, human; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D009687:Nuclear Proteins; C109540:latency-associated nuclear antigen