title: Discriminatory powers of molecular typing techniques for methicillin-resistant Staphylococcus aureus in a university hospital, Thailand.
abstract: Discriminatory powers of various molecular techniques were evaluated for typing of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Siriraj Hospital, Bangkok, Thailand. Thirty MRSA isolates were randomly selected in this study. They were characterized by pulsed-field gel electrophoresis, Clal-mecA and Clal-Tn554 polymorphisms, ribotyping, and PCR-based methods including SCCmec typing, spa and coa gene polymorphism, and repeat units in hypervariable region downstream of mecA. Individual molecular typing technique distinguished those MRSA isolates into 2 to 5 types. Eleven genetic backgrounds of MRSA isolates were elucidated by combination of typing methods with trimethoprim/sulfamethoxazole (TMP/SXT) susceptibility. Combination of all typing methods including TMP/SXT susceptibility yielded a discriminatory index of 0.94. Combination of PCR-based methods and TMP/SXT susceptibility, with the discriminatory index of 0.89, is a practical typing approach suitable for rapid epidemiological investigation of MRSA isolates in a hospital setting.
authors.affiliation: Department of Clinical Microbiology, Center for Research and Development, Medical Diagnostic Laboratory, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen , Thailand.
authors.forename: Chotechana
authors.initials: C
authors.lastname: Wilailuckana
authors.forename: Chanwit
authors.initials: C
authors.lastname: Tribuddharat
authors.forename: Chuntima
authors.initials: C
authors.lastname: Tiensasitorn
authors.forename: Pintip
authors.initials: P
authors.lastname: Pongpech
authors.forename: Penphun
authors.initials: P
authors.lastname: Naenna
authors.forename: Siriporn
authors.initials: S
authors.lastname: Rugdeekha
authors.forename: Chertsak
authors.initials: C
authors.lastname: Dhiraputra
authors.forename: Somwang
authors.initials: S
authors.lastname: Danchaivijitr
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D021521:Ribotyping; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000453:epidemiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D013785:Thailand
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial