Effects of repeated desflurane and sevoflurane anesthesia on enzymatic free radical scavanger system.
BACKGROUND
To investigate the possible effects of repeated sevoflurane and desflurane anesthesia on hepatocellular system by evaluating the free radical metabolism, hepatocellular enzymes and histopatholgical changes in rats.


METHODS
Four groups of animals were studied. Sevoflurane 2% (v/v) and desflurane 6% (v/v) in air/O2 were administered to animals in group II (n=9) and III (n=9) respectively. 100% (v/v) O(2) was administered in group IV (n=9). Administration was done for 60 minutes over 3 days. Nine animals were allocated to control group (group I), superoxide dismutase (SOD), catalase (CAT), glutathion peroxidase (GSH-Px), glutathione-s-transferase (GST) and thiobarbituric acid reactive substances (TBARS) were studied. Also electron microscopy was performed.


RESULTS
Catalase, SOD, GSH-Px, GST activities and TBARS levels were significantly higher in groups II and III than in group I (p<0.05). All parameters were significantly higher in groups II versus group IV (p<0.05). On the other hand, SOD, GSH-Px and GST activities were significantly elevated in group III than IV, but CAT activity and TBARS levels were not significantly. Catalase, SOD, GSH-Px, GST but not TBARS levels were significantly higher in groups II and III than in group IV (p<0.05). TBARS levels were higher in group III than in group IV, but this elevation was not statistically significant. CAT, SOD and GSH-Px activities were significantly higher in groups II than in group III (p<0.05).


CONCLUSIONS
Although electron microscopy findings were similar for group II and III, we can conclude that sevoflurane might cause more cellular damage than desflurane by causing higher activation of free radical metabolising enzymes.
Dikmen
Bayazit
B
Ankara Numune Hospital, Department of Anesthesiology & Reanimation, Gazi University Medical Faculty, 06510, Beşevler, Ankara, Turkiye.
Unal
Yusuf
Y
Pampal
H Kutluk
HK
Nurlu
Nilhan
N
Kurtipek
Omer
O
Canbolat
Orhan
O
Ozoğul
Candan
C
Kavutcu
Mustafa
M
D018685:Anesthetics, Inhalation / Q000633:toxicity*; D000818:Animals; D002374:Catalase / Q000378:metabolism; D000077335:Desflurane; D005609:Free Radicals / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007530:Isoflurane / Q000031:analogs & derivatives* / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D008738:Methyl Ethers / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000077149:Sevoflurane; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D018685:Anesthetics, Inhalation; D005609:Free Radicals; D008738:Methyl Ethers; D017392:Thiobarbituric Acid Reactive Substances; D000077149:Sevoflurane; D000077335:Desflurane; D007530:Isoflurane; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase