Development of an immunosensor for the diagnosis of bovine anaplasmosis.
An optical immunosensor based in major surface protein 5 (MSP5) of Anaplasma marginale was developed towards detection of anti-Anaplasma sp. antibodies in acute infection as well as in vaccinated cattle. This study was performed using recombinant MSP5 covalently immobilised in controlled pore glass (CPG) beads to detect anti-MSP5 antibodies in serum samples. The quantification is based on the measurement of the Cy5 fluorescence of the detection antibody, anti bovine IgG, after reaction with serum. Sera were collected in enzootic and tick-free regions of Argentina. The immunosensor showed a detection range of 1.2 g/ml to 48 g/ml of antibody in sera, with a sensitivity of 93% and a specificity of 70%. The optical immunosensor developed is suitable for quantification of antibodies in sera of naturally or experimentally infected animals.
Silva
Marta
M
Instituto de Biologia Experimental e Tecnológica/Instituto de Tecnologia Química e Biológica, Apartado 12, P-2781-901 Oeiras, Portugal.
Wilkowsky
Silvina
S
De Echaide
Susana Torioni
ST
Farber
Marisa
M
Oliva
Abel
A
D000372:Agglutination Tests / Q000662:veterinary; D042322:Anaplasma marginale / Q000276:immunology*; D000712:Anaplasmosis / Q000175:diagnosis* / Q000453:epidemiology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001096:Arachnid Vectors / Q000382:microbiology; D001118:Argentina / Q000453:epidemiology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007159:Immunologic Tests / Q000295:instrumentation / Q000379:methods / Q000592:standards / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013987:Ticks / Q000382:microbiology
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C111578:major surface protein 5, Anaplasma