title: Selective repression of translation by the brome mosaic virus 1a RNA replication protein.
abstract: Differential expression of viral replication proteins is essential for successful infection. We report here that overexpression of the brome mosaic virus (BMV) 1a protein can repress viral RNA replication in a dosage-dependent manner. Using RNA replication-incompetent reporter constructs, repression of translation from BMV RNA1 and RNA2 was observed, suggesting that the effect on translation of the BMV RNA replication proteins is responsible for the decrease in RNA levels. Furthermore, repression of translation by 1a required the B box in the 5'-untranslated region (5' UTR); BMV RNA3 that lacks a B box in its 5' UTR is not subject to 1a-mediated translational inhibition. Mutations in either the methyltransferase or the helicase-like domains of 1a reduced the repression of replication and translation. These results suggest that in addition to its known functions in BMV RNA synthesis, 1a also regulates viral gene expression.
authors.affiliation: Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Yi
authors.forename: K
authors.initials: K
authors.lastname: Gopinath
authors.forename: C Cheng
authors.initials: CC
authors.lastname: Kao
mesh_terms: D020121:5' Untranslated Regions / Q000502:physiology; D017795:Bromovirus / Q000235:genetics*; D015536:Down-Regulation; D015967:Gene Expression Regulation, Viral; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis*; D014026:Nicotiana; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins