title: Expression levels of CXC chemokine receptors 3 are associated with clinical phenotype of type 1 diabetes.
abstract: Type 1 diabetes is recognized as one of T helper 1 cell (Th1)-mediated diseases. The purpose of this article was to investigate the expression levels of CXC chemokine receptor 3 (CXCR3) and CC chemokine receptor 5 (CCR5) on CD4 T cells as Th1 markers in Japanese patients with type 1 diabetes and control subjects. A total of 72 patients with type 1 diabetes and 24 healthy subjects were enrolled. Their peripheral mononuclear cells were obtained and stained with anti-CXCR3, anti-CCR5, and anti-CD4 monoclonal antibodies. Flow-cytometric analysis was performed and patients were classified according to their onset pattern as fulminant, typical, or slow onset. Statistical analysis was performed using ANOVA. CXCR3 expression on CD4 T cells in patients with a fulminant pattern of onset was significantly lower than that in the other groups, and that in patients with a typical pattern of onset was significantly higher than that in the other groups. CCR5 expression on CD4 T cells was not different among the three clinical phenotypes. CXCR3 expression level is associated with the onset pattern of type 1 diabetes. Further studies are needed to clarify the role of chemokines in type 1 diabetes.
authors.affiliation: Department of Internal Medicine, Kitasato Institute Hospital, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan. yamada-s@kitasato.or.jp
authors.forename: Satoru
authors.initials: S
authors.lastname: Yamada
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Oikawa
authors.forename: Gen
authors.initials: G
authors.lastname: Sakai
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Atsumi
authors.forename: Taro
authors.initials: T
authors.lastname: Maruyama
authors.forename: Akira
authors.initials: A
authors.lastname: Shimada
mesh_terms: D000328:Adult; D017668:Age of Onset; D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016022:Case-Control Studies; D003922:Diabetes Mellitus, Type 1 / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D005819:Genetic Markers / Q000276:immunology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D019713:Receptors, CCR5 / Q000235:genetics / Q000276:immunology; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000235:genetics / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology / Q000473:pathology
chemical_list: C515187:CXCR3 protein, human; D005819:Genetic Markers; D019713:Receptors, CCR5; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine