Cap1p plays regulation roles in redox, energy metabolism and substance transport: an investigation on Candida albicans under normal culture condition.
Cap1p, a transcription factor in Candida albicans, is believed to be required for tolerance to oxidative stress. However, no information is available concerning its function on basal transcriptional profile. In this study, differentially expressed genes between the CAP1-deleted strain and its parental strain under normal culture condition were identified through microarray analysis. Notably, among the 48 down-regulated genes with the deletion of CAP1, there were three clusters, functionally related to intracellular redox, energy metabolism and substance transport. IPF7817, IPF11105 and FDH11, the three putative Cap1p target genes functionally related to redox, were shown to be activated by oxidative stress in a Cap1p-dependent manner. Furthermore, rhodamine 6G efflux analyses demonstrated that Cap1p contributed to the energy-driven efflux. Taken together, these results reveal that Cap1p plays a significant role in redox status regulation, energy metabolism and substance transport under normal culture condition.
Wang
Yan
Y
School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China.
Cao
Ying-Ying
YY
Cao
Yong-Bing
YB
Wang
De-Jun
DJ
Jia
Xin-Ming
XM
Fu
Xu-Ping
XP
Zhang
Jun-Dong
JD
Xu
Zheng
Z
Ying
Kang
K
Chen
Wan-Sheng
WS
Jiang
Yuan-Ying
YY
D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000502:physiology*; D001692:Biological Transport; D002176:Candida albicans / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D020218:Response Elements; D020133:Reverse Transcriptase Polymerase Chain Reaction
C412447:AP1 protein, Candida albicans; D050976:Basic-Leucine Zipper Transcription Factors; D005656:Fungal Proteins