[Arterial blood pressure variations: homocysteine and nitric oxide synthase gene polymorphisms (NOS3)].
BACKGROUND
The nitric oxide synthase (NOS3) G894T gene polymorphism seems as a genetic determinant of total homocysteine (tHcy) concentrations through an effect on folate catabolism. We tested for a significant contribution to blood pressure values for the NOS3 G894T and 4a/b gene polymorphisms and whether those changes could explain the modulating effect on tHcy concentrations.


METHODS
We analyzed 158 healthy men. The NOS3 gene polymorphisms were determined by polymerase chain reaction (PCR) and restriction fragment analysis (G894T) and by PCR (4a/b). Total homocysteine concentrations were evaluated by the fluorescence polarization immunoassay method.


RESULTS
In our population we did not obtain a significant contribution of the G894T to blood pressure variations. However, tHcy mean concentration values differed according G894T genotypes (P = 0.01). Interestingly, we did not obtain a significant modulating effect on tHcy concentrations according to 4a/b genotypes although the 4a/b genotype distribution was statistically associated with blood pressure variations.


CONCLUSIONS
Our results showed a modulating effect of the NOS3 4a/b gene variant on tHcy concentrations that is at least partially provoked by discrete blood pressure increments. Nevertheless, our multivariate analysis did not show a statistical significant role for the NOS3 G894T gene polymorphism on tHcy concentrations.
Rodríguez Esparragón
F J
FJ
Unidad de Investigación, Servicio de Nefrología, Hospital de Gran Canaria Dr Negrín, Universidad de Las Palmas de Gran Canaria.
Rodríguez Pérez
J C
JC
Macías Reyes
A
A
Hernández Trujillo
Y
Y
González Leiza
M
M
Caballero Hidalgo
A
A
D000328:Adult; D001794:Blood Pressure / Q000502:physiology*; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011110:Polymorphism, Genetic
D006710:Homocysteine; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III