Fabrication and characterization of semicircular detection electrodes for contactless conductivity detector - CE microchips.
This study uses simple and reliable microfabrication techniques to fabricate CE biochips, integrating a novel contactless conductivity detector in a miniaturized detection system in a microfluidic biochip. The off-channel electrodes are deposited around side channels by Au sputtering and patterned using a standard "lift-off" process. A vacuum fusion bonding process is employed to seal the lower substrate containing the microchannels and the electrodes to an upper glass cover plate. The variations in the capacitance between the semicircular detection electrodes in the side channels are measured as different samples and ions pass through the detection region of the CE separation channel. Samples of Rhodamine B, commercial sports drinks, mineral waters, and a red wine, respectively, are mixed in different buffer solutions, separated, and successfully detected using the developed device. The semicircular detection electrodes for the contactless conductivity detector have microscale dimensions and provide a valuable contribution to the realization of the lab-on-a-chip concept.
Lee
Chia-Yen
CY
Department of Mechanical and Automation Engineering, Da-Yeh University, Changhua, Taiwan.
Chen
C M
CM
Chang
Guan-Liang
GL
Lin
Che-Hsin
CH
Fu
Lung-Ming
LM
D003217:Conductometry / Q000379:methods; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry; D008839:Microelectrodes; D008900:Mineral Waters / Q000032:analysis; D012235:Rhodamines / Q000032:analysis; D014920:Wine / Q000032:analysis
D008900:Mineral Waters; D012235:Rhodamines; D006046:Gold; C029773:rhodamine B