[Evaluation of hydroalcoholic extract of Aroeira (Shinus Terebinthifolius Raddi) in the healing process of wound skin in rats].
OBJECTIVE
To evaluate the healing effect of the topic administration of the hydroalcoholic extract of aroeira in open wounds in the dorsocostal region of rats.


METHODS
Sixty Wistar rats, males, were used. In all animals one skin fragment was removed, with two centimeters in diameter. Inhalatory anesthesia with ethyl ether was done, The rats were divided in two groups of 30 animals: aroeira group, submitted to an topic application of the hydroalcoholic extract of the plant, and control group, with the same process but with 0.9% saline solution. Each group was divided in three sub-groups of 10 animals according to the period of observation: 7, 14 and 21 days. The areas of the injuries were analyzed by the macroscopic aspect and digital planimetry, and the resected segments of the wounds were studied by optic microscopy stained with hematoxilin-eosine and trichromium of Masson.


RESULTS
The macroscopic findings showed early complete reepitelization in the control group by the 14th day. According to the digital planimetry the average wound areas of the rats from the control group (0.5278 cm(2)) had been smaller than those from the wounds of the rats of the aroeira group (0.6897 cm(2)), with statistical significance to the 14th day (p=0.036). The histologic study showed statistical difference (p=0.023) regarding the mononuclear cells in 14th day of evaluation, with larger number in the aroeira group, without significant differences regarding other parameters in all the remaining days.


CONCLUSIONS
The topic use of the hydroalcoholic extract of aroeira delayed the reepitelization of the skin wounds in rats.
Branco Neto
Manoel Lages Castelo
ML
Departamento de Cirurgia, Universidade Federal do Maranhao.
Ribas Filho
Jurandir Marcondes
JM
Malafaia
Osvaldo
O
Oliveira Filho
Marco Antonio de
MA
Czeczko
Nicolau Gregori
NG
Aoki
Sonia
S
Cunha
Regina
R
Fonseca
Vinicius Ribas
VR
Teixeira
Humberto Marten
HM
Aguiar
Luiz Roberto Farion de
LR
D027926:Anacardiaceae / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D007249:Inflammation / Q000188:drug therapy; D007274:Injections, Intraperitoneal; D008297:Male; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000293:injuries* / Q000648:ultrastructure; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D000893:Anti-Inflammatory Agents; D010936:Plant Extracts