title: Ca2+ and Cu2+ supplementation increases mannitol production by Candida magnoliae.
abstract: Supplementation with CaCl(2).2H(2)O (50 mg l(-1)) or CuSO(4).5H(2)O (10 mg l(-1)) improved mannitol production by Candida magnoliae by 14.5 and 18.6% (25 and 32 g/L), respectively. When used in combination, they acted synergistically: Ca(2+) decreased the intracellular concentration of mannitol 30%, whereas Cu(2+ )increased the intracellular activity of mannitol dehydrogenase 1.6-times more than control. Ca(2+) probably works by altering the permeability of cells to mannitol, whereas, Cu(2+) increases the activity of an enzyme responsible for mannitol biosynthesis.
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, Korea.
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
authors.forename: Hee-Yeol
authors.initials: HY
authors.lastname: Song
authors.forename: In-Won
authors.initials: IW
authors.lastname: Kim
mesh_terms: D002118:Calcium / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D003300:Copper / Q000494:pharmacology*; D008353:Mannitol / Q000378:metabolism*; D008354:Mannitol Dehydrogenases / Q000378:metabolism; D009172:Mycology / Q000379:methods
chemical_list: D008353:Mannitol; D003300:Copper; D008354:Mannitol Dehydrogenases; D002118:Calcium