Expression of interleukin-10 in intestinal lymphocytes detected by an interleukin-10 reporter knockin tiger mouse.
To identify interleukin-10 (IL-10)-producing cells in vivo, we generated a knockin mouse where an internal ribosome entry site (IRES) green fluorescence protein (GFP) element was inserted immediately before the polyadenylation site of the IL-10 gene. GFP fluorescence in cells from these mice was found to correlate positively with IL-10 protein expression. With this model, we found that after multiple T cell receptor (TCR) stimulations, strong expression of IL-10 was produced specifically by intraepithelial lymphocytes (IEL) in the small intestine and colonic lamina propria lymphocytes (cLPL). We found that anti-CD3 treatment induces T regulatory cell 1 (Tr1)-like cells in small intestinal IEL (sIEL) and led to the accumulation of naturally occurring regulatory T (nTreg) cells in colonic LPL (cLPL). These findings highlight the intestine as a unique site for induction of IL-10-producing T cells, which play a critical role in the regulation of inflammation in the gut.
Kamanaka
Masahito
M
Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Kim
Sean T
ST
Wan
Yisong Y
YY
Sutterwala
Fayyaz S
FS
Lara-Tejero
Maria
M
Galán
Jorge E
JE
Harhaj
Ed
E
Flavell
Richard A
RA
D019264:Adoptive Transfer; D000818:Animals; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000276:immunology / Q000378:metabolism; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D018928:Immunity, Mucosal; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000276:immunology; D007422:Intestines / Q000166:cytology / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology*; D051379:Mice; D008817:Mice, Mutant Strains; D013601:T-Lymphocytes / Q000276:immunology*
D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D016753:Interleukin-10; D049452:Green Fluorescent Proteins