Exploration of members of Aspergillus sections Nigri, Flavi, and Terrei for feruloyl esterase production.
The ability of members of Aspergillus sections Nigri, Flavi, and Terrei to produce feruloyl esterases was studied according to their substrate specificity against synthetic methyl esters of hydroxycinnamic acids. Type A feruloyl esterases (FAEA), induced during growth on cereal-derived products, show a preference for the phenolic moiety of substrates that contain methoxy substitutions, as found in methyl sinapinate, whereas type B feruloyl esterases (FAEB) show a preference for the phenolic moiety of substrates that contain hydroxyl substitutions, as occurs in methyl caffeate. All the strains of Aspergillus section Nigri (e.g., A. niger and A. foetidus) were able to produce feruloyl esterases with activity profiles similar to those reported for FAEA and FAEB of A. niger when grown on oat-spelt xylan and sugar beet pulp, respectively. The two genes encoding these proteins, faeA and faeB, were identified by Southern blot analysis. The strains of Aspergillus sections Flavi (e.g., A. flavus, A. flavo-furcatus, and A. tamarii) and Terrei (e.g., A. terreus) were able to produce type A and type B enzymes. faeA was revealed in genomic DNA of these strains, and FAEA was determined by immunodetection in cultures grown in oat-spelt xylan. In addition, type B enzymes, not related to faeB, were efficiently induced by oat-spelt xylan and exhibited very original activity profiles on sugar beet pulp. This work confirms that the members of the genus Aspergillus are good feruloyl esterase producers.
Bouzid
Ourdia
O
Biotechnologie de champignons filamenteux, INRA/Universités de Provence et de la Méditerranée, IFR86 de Biotechnologie agro-industrielle de Marseille, ESIL, France.
Record
Eric
E
Asther
Michèle
M
Haon
Mireille
M
Navarro
David
D
Asther
Marcel
M
Lesage-Meessen
Laurence
L
D001230:Aspergillus / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D001231:Aspergillus flavus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D015139:Blotting, Southern; D015153:Blotting, Western; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D013379:Substrate Specificity; D013997:Time Factors
D017077:Culture Media, Conditioned; D007527:Isoenzymes; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase