title: Sensitive and specific method for detecting G protein-coupled receptor mRNAs.
abstract: G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.
authors.affiliation: Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institute of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA. arnehansen@nidcr.nih.gov
authors.forename: Arne
authors.initials: A
authors.lastname: Hansen
authors.forename: Yidong
authors.initials: Y
authors.lastname: Chen
authors.forename: Jason M
authors.initials: JM
authors.lastname: Inman
authors.forename: Quang N
authors.initials: QN
authors.lastname: Phan
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: Qi
authors.forename: Charlie C
authors.initials: CC
authors.lastname: Xiang
authors.forename: Miklos
authors.initials: M
authors.lastname: Palkovits
authors.forename: Natasha
authors.initials: N
authors.lastname: Cherman
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Kuznetsov
authors.forename: Pamela G
authors.initials: PG
authors.lastname: Robey
authors.forename: Eva
authors.initials: E
authors.lastname: Mezey
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Brownstein
mesh_terms: D002460:Cell Line; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled