title: Ferredoxin/ferredoxin-thioredoxin reductase complex: Complete NMR mapping of the interaction site on ferredoxin by gallium substitution.
abstract: The reduction of ferredoxin-thioredoxin reductase (FTR) by plant-type ferredoxin plays an important role in redox regulation in plants and cyanobacteria. Nuclear magnetic resonance (NMR) was used to map the binding sites on Synechocystis ferredoxin for FTR. A gallium-substituted structural analog of this [2Fe-2S] ferredoxin was obtained by reconstituting the apoprotein in a refolding buffer containing gallium. For the first time, the complete interaction interface of a [2Fe-2S] ferredoxin with a target enzyme has been mapped by NMR chemical shift perturbation with this diamagnetic structural analog.
authors.affiliation: Leiden Institute of Chemistry, Leiden University, Gorlaeus Laboratories, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
authors.forename: Xingfu
authors.initials: X
authors.lastname: Xu
authors.forename: Sung-Kun
authors.initials: SK
authors.lastname: Kim
authors.forename: Peter
authors.initials: P
authors.lastname: Schürmann
authors.forename: Masakazu
authors.initials: M
authors.lastname: Hirasawa
authors.forename: Jatindra N
authors.initials: JN
authors.lastname: Tripathy
authors.forename: Jody
authors.initials: J
authors.lastname: Smith
authors.forename: David B
authors.initials: DB
authors.lastname: Knaff
authors.forename: Marcellus
authors.initials: M
authors.lastname: Ubbink
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D005288:Ferredoxins / Q000737:chemistry / Q000378:metabolism*; D005708:Gallium / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D046939:Synechocystis / Q000201:enzymology*; D017186:Titrimetry
chemical_list: D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; D005708:Gallium; D010088:Oxidoreductases; C021588:ferredoxin-thioredoxin reductase