title: RNA interference of Hemolin causes depletion of phenoloxidase activity in Hyalophora cecropia.
abstract: Melanization is regulated by the prophenoloxidase cascade and functions as a response to intruding microorganisms in invertebrates. When injecting dsRNA of the lepidopteran immune protein hemolin in pupae of Hyalophora cecropia (Lepidoptera: Saturniidae), we observed a significant reduction in phenoloxidase activity after 24 h, but not after 72 h. The link between hemolin and the prophenoloxidase system suggests that hemolin is a pattern recognition protein important for the triggering of the prophenoloxidase cascade in the defence against bacterial infections.
authors.affiliation: Department of Genetics, Microbiology and Toxicology, Stockholm University, 106 91 Stockholm, Sweden.
authors.forename: Olle
authors.initials: O
authors.lastname: Terenius
authors.forename: Raul
authors.initials: R
authors.lastname: Bettencourt
authors.forename: So Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Wenli
authors.initials: W
authors.lastname: Li
authors.forename: Kenneth
authors.initials: K
authors.lastname: Söderhäll
authors.forename: Ingrid
authors.initials: I
authors.lastname: Faye
mesh_terms: D000818:Animals; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007915:Lepidoptera / Q000201:enzymology* / Q000276:immunology*; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D034622:RNA Interference / Q000276:immunology*
chemical_list: D007136:Immunoglobulins; D019476:Insect Proteins; C066846:hemolin; D014442:Monophenol Monooxygenase