Conformational restriction blocks glutamate receptor desensitization.
Desensitization is a universal feature of ligand-gated ion channels. Using the crystal structure of the GluR2 L483Y mutant channel as a guide, we attempted to build non-desensitizing kainate-subtype glutamate receptors. Success was achieved for GluR5, GluR6 and GluR7 with intermolecular disulfide cross-links but not by engineering the dimer interface. Crystallographic analysis of the GluR6 Y490C L752C dimer revealed relaxation from the active conformation, which functional studies reveal is not sufficient to trigger desensitization. The equivalent non-desensitizing cross-linked GluR2 mutant retained weak sensitivity to a positive allosteric modulator, which had no effect on GluR2 L483Y. These results establish that the active conformation of AMPA and kainate receptors is conserved and further show that their desensitization requires dimer rearrangements, that subtle structural differences account for their diverse functional properties and that the ligand-binding core dimer is a powerful regulator of ion-channel activity.
Weston
Matthew C
MC
Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA.
Schuck
Peter
P
Ghosal
Alokesh
A
Rosenmund
Christian
C
Mayer
Mark L
ML
D000595:Amino Acid Sequence; D002460:Cell Line; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D004229:Dithiothreitol; D004594:Electrophysiology; D006801:Humans; D008024:Ligands; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D015202:Protein Engineering; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D017470:Receptors, Glutamate / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D040681:Structural Homology, Protein; D014443:Tyrosine / Q000235:genetics / Q000378:metabolism
D004220:Disulfides; D008024:Ligands; D017470:Receptors, Glutamate; D014443:Tyrosine; D008239:Lysine; D004229:Dithiothreitol