title: Amygdala adenosine A1 receptors have no anticonvulsant effect on piriform cortex-kindled seizures in rat.
abstract: Adenosine is an endogenous anticonvulsant that exerts its effects through A1 receptors. As the piriform/amygdala is a critical circuit for limbic seizure propagation, in this study, the role of basolateral amygdala A1 receptors on piriform cortex (PC)-kindled seizures was investigated. Rats were kindled by daily electrical stimulation of PC. In fully kindled animals, bilateral intra-amygdala N6-cyclohexyladenosine (CHA; 10-500 micromol/L, a selective A1 receptor agonist) had no effect on kindled-seizure parameters. However, bilateral intra-amygdala 2% lidocaine (reversal neuronal inhibitor) reduced the kindled seizure severity. There was significant increase in stage 4 latency and decrease in stage 5 duration. Bilateral lesion of basolateral amygdala of kindled animals (by electrical DC current) reduced the kindled seizure severity more dramatically. Our results showed afterdischarge duration, stage 5 duration, and seizure duration were decreased and stage 4 latency increased significantly. In addition, daily intra-amygdala CHA had no significant effect on PC kindling acquisition. Therefore, it may be concluded that although the basolateral amygdala neuronal activity has a critical role in the propagation of epileptic seizures from PC, the amygdala A1 receptors have no role in this regard. On the other hand, amygdala A1 receptors have no anticonvulsant or antiepileptogenic effect on PC-kindled seizures.
authors.affiliation: Department of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, I.R. Iran.
authors.forename: Parviz
authors.initials: P
authors.lastname: Shahabi
authors.forename: Javad
authors.initials: J
authors.lastname: Mirnajafi-Zadeh
authors.forename: Yaghoub
authors.initials: Y
authors.lastname: Fathollahi
authors.forename: Narges
authors.initials: N
authors.lastname: Hoseinmardi
authors.forename: Mohammad Ebrahim
authors.initials: ME
authors.lastname: Rezvani
authors.forename: Ali
authors.initials: A
authors.lastname: Eslami-far
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D058907:Adenosine A1 Receptor Agonists; D000679:Amygdala / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D004558:Electric Stimulation; D008012:Lidocaine / Q000494:pharmacology; D008297:Male; D009833:Olfactory Pathways / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D043682:Receptor, Adenosine A1 / Q000502:physiology*; D012640:Seizures / Q000503:physiopathology*
chemical_list: D058907:Adenosine A1 Receptor Agonists; D043682:Receptor, Adenosine A1; C027513:N(6)-cyclohexyladenosine; D008012:Lidocaine; D000241:Adenosine