Oxygen transfer model in recombinant Pichia pastoris and its application in biomass estimation.
An oxygen transfer model was established for Pichia pastoris growing on glycerol and methanol in a stirred tank bioreactor and expressing a recombinant human serum albumin (rHSA). This was based on pseudo-steady state mass balance, where the volumetric O(2) transfer coefficient, k (L) a, was estimated as a function of power input per unit volume and aeration rate. Under pseudo-steady state, the O(2) transfer rate model matched the O(2) uptake rate obtained from a previous macrokinetic model. This procedure was also applied to estimate biomass concentration by using the on-line rolling identification approach.
Liang
Jieming
J
Department of Automation, Shanghai Jiao Tong University, 800 Dongchuan Lu, Shanghai, PR China.
Yuan
Jingqi
J
D019149:Bioreactors / Q000382:microbiology*; D049109:Cell Proliferation; D003198:Computer Simulation; D006801:Humans; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010843:Pichia / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis; D012709:Serum Albumin / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins; D012709:Serum Albumin; D010100:Oxygen