Evaluation of sex differences on mitochondrial bioenergetics and apoptosis in mice.
It has been postulated that the differences in longevity observed between organisms of different sexes within a species can be attributed to differences in oxidative stress. It is generally accepted that differences are due to the higher female estrogen levels. However, in some species males live the same or longer despite their lower estrogen values. Therefore, in the present study, we analyze key parameters of mitochondrial bioenergetics, oxidative stress and apoptosis in the B6 (C57Bl/6J) mouse strain. There are no differences in longevity between males and females in this mouse strain, although estrogen levels are higher in females. We did not find any differences in heart, skeletal muscle and liver mitochondrial oxygen consumption (State 3 and State 4) and ATP content between male and female mice. Moreover, mitochondrial H(2)O(2) generation and oxidative stress levels determined by cytosolic protein carbonyls and concentration of 8-hydroxy-2'-deoxyguanosine in mitochondrial DNA were similar in both sexes. In addition, markers of apoptosis (caspase-3, caspase-9 and mono- and oligonucleosomes: the apoptosis index) were not different between male and female mice. These data show that there are no differences in mitochondrial bioenergetics, oxidative stress and apoptosis due to gender in this mouse strain according with the lack of differences in longevity. These results support the Mitochondrial Free Radical Theory of Aging, and indicate that oxidative stress generation independent of estrogen levels determines aging rate.
Sanz
Alberto
A
Department of Animal Physiology-II, Faculty of Biology, Complutense University, Madrid 28040, Spain. albsanz@bio.ucm.es
Hiona
Asimina
A
Kujoth
Gregory C
GC
Seo
Arnold Y
AY
Hofer
Tim
T
Kouwenhoven
Evelyn
E
Kalani
Rizwan
R
Prolla
Tomas A
TA
Barja
Gustavo
G
Leeuwenburgh
Christiaan
C
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015415:Biomarkers / Q000032:analysis; D020169:Caspases / Q000032:analysis; D004272:DNA, Mitochondrial / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D008099:Liver / Q000378:metabolism; D008136:Longevity / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D012737:Sex Factors
D015415:Biomarkers; D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species; D020169:Caspases