Molecular epidemiological analysis of Newcastle disease virus isolated in China in 2005.
Eighty-three strains of Newcastle disease virus (NDV) were obtained from outbreaks in chickens, pigeons, geese, and ducks in China in 2005 and characterized genotypically. The main functional region of the F gene (535 nucleotides) was amplified and sequenced. A phylogenetic tree based on nucleotides 47-435 of the F gene was created using sequences from 83 isolates and representative NDV sequences obtained from GenBank. Phylogenetic analysis showed that all newly characterized strains belonged to six genetic groups: I, II, III, VIb, VIIc, and VIId. All the isolates belonging to groups I and II (14 total) were lentogenic according to the amino acid sequences of the fusion protein cleavage site, and either V4 or LaSota-type, depending on the vaccines that were used. Most isolates (64 total) were classified in group VIId, a predominant genotype responsible for most Newcastle disease outbreaks since the end of the last century. One strain, NDV05-055, was in group VIIc, three pigeon strains were in group VIb, and one isolate, NDV05-041, was in group III, and characterized as a velogenic strain. This study revealed that genotype VIId was the major NDV strain responsible for the 2005 ND epizoonosis that occurred in China.
Liu
Hualei
H
National Reference Laboratory for Newcastle Disease, China Animal Health and Epidemiology Center, Qingdao 266032, China.
Wang
Zhiliang
Z
Wu
Yangong
Y
Zheng
Dongxia
D
Sun
Chengying
C
Bi
Daorong
D
Zuo
Yuanyuan
Y
Xu
Tiangang
T
D000818:Animals; D002645:Chickens / Q000821:virology; D002681:China / Q000453:epidemiology; D010856:Columbidae / Q000821:virology; D004372:Ducks / Q000821:virology; D005777:Geese / Q000821:virology; D017720:Molecular Epidemiology; D009521:Newcastle Disease / Q000453:epidemiology / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000235:genetics / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014760:Viral Fusion Proteins / Q000235:genetics*
D012367:RNA, Viral; D014760:Viral Fusion Proteins