title: Retrotransposon-based S-SAP as a platform for the analysis of genetic variation and linkage in globe artichoke.
abstract: A high copy number of retrotransposon sequences are present and widely dispersed in plant genomes. Their activity generates a considerable degree of sequence polymorphism. Here, we report the cloning of CYRE-5, a long-terminal repeat carrying retrotransposon-like sequence in Cynara cardunculus L., and its exploitation to develop a DNA fingerprinting assay across 22 accessions, including both cultivated (globe artichoke and cultivated cardoon) and wild (wild cardoon) types. The effectiveness of the sequence-specific amplified polymorphism (S-SAP) platform is compared with that of amplified fragment length polymorphism (AFLP). A genetic linkage analysis, based on a hybrid population between 2 globe artichoke varietal types, resulted in the inclusion of 29 S-SAP loci in the core genetic map, confirming their dispersed distribution across the globe artichoke genome.
authors.affiliation: Di.Va.P.R.A. Plant Genetics and Breeding, University of Turin, via L. da Vinci 44, I-10095 Grugliasco (Turin), Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Acquadro
authors.forename: Ezio
authors.initials: E
authors.lastname: Portis
authors.forename: Andrea
authors.initials: A
authors.lastname: Moglia
authors.forename: Franco
authors.initials: F
authors.lastname: Magurno
authors.forename: Sergio
authors.initials: S
authors.lastname: Lanteri
mesh_terms: D031943:Cynara scolymus / Q000235:genetics*; D008040:Genetic Linkage; D014644:Genetic Variation; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D018626:Retroelements
chemical_list: D018626:Retroelements