Combining stir bar sorptive extraction and MEKC for the determination of polynuclear aromatic hydrocarbons in environmental and biological matrices.
In this work, stir bar sorptive extraction and liquid desorption was combined with MEKC and diode-array detection (SBSE-LD-MEKC-DAD) for the determination of polynuclear aromatic hydrocarbons (PAHs) in aqueous medium, using biphenyl, fluorene, anthracene, phenanthrene, fluoranthene and pyrene as model compounds. MEKC-DAD conditions and parameters affecting SBSE-LD efficiency are fully discussed. Assays performed on aqueous samples spiked at trace levels, yielded recoveries ranging from 55.5 +/- 6.1% (pyrene) to 70.7 +/- 7.1% (anthracene), under optimized experimental conditions. The methodology proved to be nearly described by the octanol-water partition coefficients (K(PDMS/W) approximately K(O/W)). The analytical performance showed good precision (<12.0%), suitable detection limits (2-11 microg/L) and convenient linear dynamic ranges (r(2)>0.99) from 5 to 25 microg/L for anthracene and 25 to 125 microg/L for the remaining compounds. The application of the proposed methodology to environmental water, sediments and fish bile matrices demonstrated good selectivity and accuracy. SBSE-LD combined with MEKC-DAD was shown to be an easy, reliable and robustness methodology, as well as a good analytical alternative to monitor environmental priority pollutants.
do Rosário
Pedro M A
PM
Chemistry and Biochemistry Department, University of Lisbon, Lisbon, Portugal.
Nogueira
José M F
JM
D000818:Animals; D001646:Bile / Q000737:chemistry; D012634:Bodily Secretions / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary; D005399:Fishes; D019015:Geologic Sediments / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000379:methods*; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000302:isolation & purification
D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D014867:Water