title: Evaluation of purified Taenia solium glycoproteins and recombinant antigens in the serologic detection of human and swine cysticercosis.
abstract: Cysticercosis caused by infection with embryonated eggs of Taenia solium is an important cause of neurological disease worldwide. On the basis of mitochondrial DNA analysis, T. solium is divided into 2 (African/American and Asian) genotypes. Glycoproteins (GPs) in cyst fluid purified from the 2 genotypes of T. solium were characterized and compared with the recombinant chimeric T. solium-Ag1V1/Ag2 protein (Rec-Ag1V1/Ag2) as serodiagnostic antigens. Immunoblot analysis revealed that banding patterns of GPs differed between the 2 genotypes because of posttranslation modification, especially glycosylation. The comparison of native GPs with Rec-Ag1V1/Ag2 by enzyme-linked immunosorbent assay demonstrated that there was no statistical difference in sensitivity. In addition, the conservation of the genes encoding Ag1V1 and Ag2 in T. solium worldwide was verified. These results indicate that Rec-Ag1V1/Ag2 has great potential for usefulness in serodiagnosis as an alternative to native antigens.
authors.affiliation: Department of Parasitology, Asahikawa Medical College, Asahikawa-Hokkaido, Japan.
authors.forename: Marcello Otake
authors.initials: MO
authors.lastname: Sato
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sako
authors.forename: Minoru
authors.initials: M
authors.lastname: Nakao
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasaki
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakaya
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000349:Africa; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D001208:Asia; D015153:Blotting, Western; D003551:Cysticercosis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology*; D006031:Glycosylation; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D016415:Sequence Alignment; D013020:South America; D013045:Species Specificity; D013552:Swine; D041201:Taenia solium / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D006023:Glycoproteins; D015801:Helminth Proteins; D007106:Immune Sera; D011994:Recombinant Proteins