title: In vitro biocompatibility evaluation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer in fibroblast cells.
abstract: Among the various biomaterials available for tissue engineering and therapeutic applications, microbial polyhydroxyalkanoates offer the most diverse range of thermal and mechanical properties. In this study, the biocompatibility of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB); containing 50 mol % of 4-hydroxybutyrate] copolymer produced by Delftia acidovorans was evaluated. The cytotoxicity, mode of cell death, and genotoxicity of P(3HB-co-4HB) extract against V79 and L929 fibroblast cells were assessed using MTT assay, acridine orange/propidium iodide staining, and alkaline comet assay, respectively. Our results demonstrate that P(3HB-co-4HB) treated on both cell lines were comparable with clinically-used Polyglactin 910, where more than 60% of viable cells were observed following 72-h treatment at 200 mg/mL. Further morphological investigation on the mode of cell death showed an increase in apoptotic cells in a time-dependent manner in both cell lines. On the other hand, P(3HB-co-4HB) at 200 mg/mL showed no genotoxic effects as determined by alkaline comet assay following 72-h treatment. In conclusion, our study indicated that P(3HB-co-4HB) compounds showed good biocompatibility in fibroblast cells suggesting that it has potential to be used for future medical applications.
authors.affiliation: Biomedical Science Department, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia.
authors.forename: Ee Ling
authors.initials: EL
authors.lastname: Siew
authors.forename: Nor Fadilah
authors.initials: NF
authors.lastname: Rajab
authors.forename: Annuar Bin
authors.initials: AB
authors.lastname: Osman
authors.forename: Kumar
authors.initials: K
authors.lastname: Sudesh
authors.forename: Salmaan Hussain
authors.initials: SH
authors.lastname: Inayat-Hussain
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D020552:Comet Assay; D006224:Cricetinae; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006885:Hydroxybutyrates / Q000494:pharmacology* / Q000633:toxicity; D008422:Materials Testing; D051379:Mice; D011091:Polyesters / Q000494:pharmacology* / Q000633:toxicity; D023822:Tissue Engineering; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D006885:Hydroxybutyrates; D011091:Polyesters; C068056:poly(3-hydroxybutyrate-co-4-hydroxybutyrate); D000094122:Polyhydroxybutyrates