title: Effects of mutations in the GanB/RgsA G protein mediated signalling on the autolysis of Aspergillus nidulans.
abstract: Physiological changes taking place in carbon-starved, autolysing cultures of Aspergillus (Emericella) nidulans strains with mutations in the GanB/RgsA heterotrimeric G protein signalling pathway were studied and compared. Deletion of the ganB, rgsA or both genes did not alter markedly either the autolytic loss of biomass or the extracellular chitinase production. However, they caused a significant decrease in the proteinase formation, which was detected by measuring both extracellular enzyme activity and the transcription of the prtA gene. The deletion mutants also showed significantly higher specific gamma -glutamyltranspeptidase activities than the control strain. Deletion of the rgsA gene affected the glutathione peroxidase and catalase formation, as well as the peroxide content of the cells. The concomitant initiations of cell death and developmental genomic programmes may be interconnected via heterotrimeric G-protein signalling and subsequent changes in intracellular ROS levels in ageing A. nidulans.
authors.affiliation: Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen, H-4010 Debrecen, P.O. Box: 63, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Molnár
authors.forename: Tamás
authors.initials: T
authors.lastname: Emri
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Zavaczki
authors.forename: Tünde
authors.initials: T
authors.lastname: Pusztahelyi
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism; D020555:GTP-Binding Protein Regulators / Q000235:genetics* / Q000378:metabolism; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D009154:Mutation; D010447:Peptide Hydrolases / Q000378:metabolism; D020710:RGS Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D020555:GTP-Binding Protein Regulators; D044385:GTP-Binding Protein alpha Subunits; D020710:RGS Proteins; D002374:Catalase; D005979:Glutathione Peroxidase; D005723:gamma-Glutamyltransferase; D002688:Chitinases; D010447:Peptide Hydrolases; D005947:Glucose