title: Transporter assays using solid supported membranes: a novel screening platform for drug discovery.
abstract: Transporters are important targets in drug discovery. However, high throughput-capable assays for this class of membrane proteins are still missing. Here we present a novel drug discovery platform technology based on solid supported membranes. The functional principles of the technology are described, and a sample selection of transporter assays is discussed: the H(+)-dependent peptide transporter PepT1, the gastric proton pump, and the Na(+)/Ca(2+) exchanger. This technology promises to have an important impact on the drug discovery process.
authors.affiliation: IonGate Biosciences, Frankfurt, Germany.
authors.forename: Bela
authors.initials: B
authors.lastname: Kelety
authors.forename: Kerstin
authors.initials: K
authors.lastname: Diekert
authors.forename: Joanna
authors.initials: J
authors.lastname: Tobien
authors.forename: Natalie
authors.initials: N
authors.lastname: Watzke
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Dörner
authors.forename: Petr
authors.initials: P
authors.lastname: Obrdlik
authors.forename: Klaus
authors.initials: K
authors.lastname: Fendler
mesh_terms: D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D015195:Drug Design; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D026901:Membrane Transport Proteins / Q000737:chemistry*; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods
chemical_list: D026901:Membrane Transport Proteins; D008567:Membranes, Artificial