Characterization of red cell membrane proteins as a function of red cell density: annexin VII in different forms of hereditary spherocytosis.
Fresh human blood samples were collected from healthy controls and splenectomized and unsplenectomized patients with hereditary spherocytosis due to band 3 or ankyrin and spectrin deficiency. The erythrocytes were separated into age-related fractions using self-forming Percoll density gradients. Membrane proteins were analysed by 2D electrophoresis and identified by mass spectrometry. Annexin VII was present in reticulocytes but was then lost as the cells matured. A different pattern was found in band 3-deficient samples: annexin VII was in fact present in both mature and immature red cell membranes. Cytoskeletal anomalies may then influence the turn-over of annexin VII during erythrocyte maturation.
Caterino
Marianna
M
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli "Federico II", Via Pansini, 5 80131 Napoli, Italy.
Ruoppolo
Margherita
M
Orrù
Stefania
S
Savoia
Marcella
M
Perrotta
Silverio
S
Del Vecchio
Luigi
L
Salvatore
Francesco
F
Stewart
Gordon W
GW
Iolascon
Achille
A
D001457:Anion Exchange Protein 1, Erythrocyte / Q000172:deficiency; D017487:Ankyrins / Q000172:deficiency; D017310:Annexin A7 / Q000378:metabolism*; D002452:Cell Count; D015180:Electrophoresis, Gel, Two-Dimensional; D004905:Erythrocyte Aging; D004910:Erythrocyte Membrane / Q000737:chemistry*; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry; D021381:Protein Transport; D013049:Spectrin / Q000172:deficiency; D013103:Spherocytosis, Hereditary / Q000378:metabolism*
D001457:Anion Exchange Protein 1, Erythrocyte; D017487:Ankyrins; D017310:Annexin A7; D008565:Membrane Proteins; D013049:Spectrin