Interleukin-1beta and interleukin-6 stimulate matrix metalloproteinase-9 secretion in cultured myenteric glia.
Western blotting of culture media of myenteric glia stained positive for GFAP revealed increased secretion of matrix metalloproteinase-9 (MMP-9) in interleukin-1beta (IL-1beta) stimulated cells (10 ng/mL) versus control (142 +/- 19 versus 42 +/- 19, P < 0.05). Interleukin-6 (IL-6) stimulated cells also showed increased expression of MMP-9 (10 ng/mL) versus control (69 +/- 14 versus 4 +/- 2, P < 0.01). Control and cytokine-stimulated cells secreted MMP-2 constituitively. Gelatin zymography demonstrated that products were biologically active. Cytoplasmic staining for MMP-9 was detected in IL-1beta and IL-6-stimulated cells but was negligible in controls. Cultured myenteric glia are responsive to IL-1beta and IL-6 stimulation by secreting MMPs into the extracellular environment.
Lin
Theodore
T
Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, USA.
Zhang
Weizhen
W
Fan
Yongyi
Y
Mulholland
Michael
M
D000818:Animals; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism; D005110:Extracellular Space / Q000201:enzymology / Q000276:immunology; D006168:Guinea Pigs; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D009197:Myenteric Plexus / Q000166:cytology* / Q000276:immunology; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D015213:Neuroimmunomodulation / Q000502:physiology*
D017077:Culture Media, Conditioned; D053583:Interleukin-1beta; D015850:Interleukin-6; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9