title: Sum-frequency generation spectroscopy of DNA monolayers.
abstract: The anchoring of thiolated single-stranded DNA (HS-ssDNA) monolayers onto platinum substrates was investigated by sum-frequency generation spectroscopy. Different buffer solutions were used for the preparation of the adlayers. Vibrational fingerprints in the 2700-3100 cm(-1) spectral range showed the intercalation of Tris/EDTA (TE) buffer molecules within the HS-ssDNA self-assembled monolayer. Buffer contribution to SFG can be quenched either by using SFG inactive molecules like KH(2)PO(4)/K(2)HPO(4)/NaCl (PBS) or by repeated rinsing of the DNA layer with pure water. Comparing the SFG spectra of HS-ssDNA and mercaptohexanol (MCH), which had been self-assembled onto the same substrate, enabled us to infer ordering of the anchor arms and strong disordering of the DNA strands of HS-ssDNA monolayers self-assembled on platinum.
authors.affiliation: Laboratoire de Spectroscopie Moléculaire de Surface, Université de Namur, 61 rue de Bruxelles, B-5000 Namur, Belgium. yannick.sartenaer@fundp.ac.be
authors.forename: Yannick
authors.initials: Y
authors.lastname: Sartenaer
authors.forename: Gérard
authors.initials: G
authors.lastname: Tourillon
authors.forename: Laurent
authors.initials: L
authors.lastname: Dreesen
authors.forename: Dan
authors.initials: D
authors.lastname: Lis
authors.forename: Alaa A
authors.initials: AA
authors.lastname: Mani
authors.forename: Paul A
authors.initials: PA
authors.lastname: Thiry
authors.forename: André
authors.initials: A
authors.lastname: Peremans
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D004247:DNA