title: A single-nucleotide synonymous mutation in the gag gene controlling human immunodeficiency virus type 1 virion production.
abstract: Viral factors as well as host ones play major roles in the disease progression of human immunodeficiency virus type 1 (HIV-1) infection. We have examined cytotoxic T-lymphocyte activity and HIV-1 DNA PCR results of 312 high-risk seronegative drug users in northern Thailand and identified four seronegative cases positive for both assays. Furthermore, we have identified a synonymous mutation in nucleotide position 75 of the gag p17 gene (A426G) of HIV-1 that belongs to the CRF01_AE virus circulating in Thailand. The replication-competent HIV-1 clone containing the A426G mutation demonstrated a dramatic reduction of virion production and perturbation of viral morphogenesis without affecting viral protein synthesis in cells.
authors.affiliation: National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
authors.forename: Takaichi
authors.initials: T
authors.lastname: Hamano
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Matsuo
authors.forename: Yurina
authors.initials: Y
authors.lastname: Hibi
authors.forename: Ann Florence B
authors.initials: AF
authors.lastname: Victoriano
authors.forename: Naoko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Yosio
authors.initials: Y
authors.lastname: Mabuchi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Soji
authors.forename: Shinji
authors.initials: S
authors.lastname: Irie
authors.forename: Pathom
authors.initials: P
authors.lastname: Sawanpanyalert
authors.forename: Hideki
authors.initials: H
authors.lastname: Yanai
authors.forename: Takashi
authors.initials: T
authors.lastname: Hara
authors.forename: Shudo
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Okamoto
mesh_terms: D004279:DNA, Viral / Q000235:genetics; D015750:Genes, gag / Q000235:genetics / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D009154:Mutation; D009711:Nucleotides; D014771:Virion / Q000502:physiology*
chemical_list: D004279:DNA, Viral; D009711:Nucleotides