title: [Encapsulating hepatocytes with chitosan in physiological conditions].
abstract: Prepared from 15.3% N-acetylated chitosan (FNC), half N-acetylated chitosan (HNC) possesses a good solubility in a weak basic solution, guaranteeing the formation of microcapsules by the coacervating reaction between HNC and methacrylic acid (MAA)-hydroxyethyl methacrylate (HEMA)-methyl methacrylate (MMA) (MAA-HEMA-MMA) terpolymer under physiological conditions. When hepatocytes were encapsulated in such 3-dimensional microenvironment, as compared to monolayer culture, cell functions, including P450 activity, urea production and albumin release, were well supported. The prepared microcapsules have good mechanical stability and permeability.
authors.affiliation: Department of Chemical Engineering, Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330029, China. envzjh@ncu.edu.cn
authors.forename: Jianhang
authors.initials: J
authors.lastname: Zhu
authors.forename: Bao
authors.initials: B
authors.lastname: Zhang
authors.forename: Xiluan
authors.initials: X
authors.lastname: Yan
authors.forename: Xuejun
authors.initials: X
authors.lastname: Lao
authors.forename: Hanry
authors.initials: H
authors.lastname: Yu
mesh_terms: D000818:Animals; D002214:Capsules; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008689:Methacrylates; D020366:Methylmethacrylate; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods
chemical_list: D002214:Capsules; D008689:Methacrylates; D011108:Polymers; D020366:Methylmethacrylate; C008384:methacrylic acid; C005044:hydroxyethyl methacrylate; D048271:Chitosan