title: Preparation of monoclonal antibodies for detection and identification of Francisella tularensis.
abstract: Monoclonal antibodies (MAbs) against Francisella tularensis were obtained. Three MAbs specifically reacted with F. tularensis, while four MAbs reacted with other members of the genus Francisella as well. Fluorescent isothiocyanate-conjugated MAbs unequivocally stained bacterial cells in specimens from experimentally infected mice. Two MAbs agglutinated F. tularensis antigen in the agglutination tests. These MAbs should improve methods for detection and identification of F. tularensis.
authors.affiliation: Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Tokyo 162-8640, Japan. ahotta@nih.go.jp
authors.forename: Akitoyo
authors.initials: A
authors.lastname: Hotta
authors.forename: Akihiko
authors.initials: A
authors.lastname: Uda
authors.forename: Osamu
authors.initials: O
authors.lastname: Fujita
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Tanabayashi
authors.forename: Akio
authors.initials: A
authors.lastname: Yamada
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D005604:Francisella tularensis / Q000276:immunology / Q000302:isolation & purification*; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014406:Tularemia / Q000175:diagnosis
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal