Analgesic activity of affinin, an alkamide from Heliopsis longipes (Compositae).
Heliopsis longipes (Compositae) is a Mexican plant used as analgesic in pain toothache. A solution of 10mug/ml of dichloromethane extract from this plant showed analgesic activity determined by means of GABA release in mice brain slices. Through a bioassay-directed separation, fractions G-1, G-2, G-4 and G-6 at the same concentration were active. Affinin was the unique and common active compound, and evoke the GABA release 0.5min after administration at 1x10(-4)M concentration. Inactive compound were undeca-2E-en-8,10-dyinoic acid isobutylamide, hinokinin, 2'-hydroxyhinokinin, 3beta-sn-glyceroyl-(1''-palmitoxy)urs-12-ene, 13(18)-ursen-3beta-ol, 13(18)-ursen-3beta-acetate, beta-sitosterol and stigmasterol. The analgesic activity of Heliopsis longipes could be associated to affinin.
Rios
María Yolanda
MY
Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, Mexico. myolanda@uaem.mx
Aguilar-Guadarrama
A Berenice
AB
Gutiérrez
María Del Carmen
Mdel C
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000302:isolation & purification; D000475:Alkenes / Q000302:isolation & purification / Q000494:pharmacology*; D000577:Amides / Q000302:isolation & purification / Q000494:pharmacology*; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D052117:Benzodioxoles; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004149:Dioxoles / Q000302:isolation & purification; D005260:Female; D066298:In Vitro Techniques; D017705:Lignans / Q000302:isolation & purification; D008519:Medicine, Traditional; D008752:Methylene Chloride; D008800:Mexico; D051379:Mice; D010146:Pain / Q000188:drug therapy*; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D053284:Polyunsaturated Alkamides / Q000302:isolation & purification / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000187:drug effects / Q000378:metabolism
D000475:Alkenes; D000577:Amides; D000700:Analgesics; D052117:Benzodioxoles; D004149:Dioxoles; D017705:Lignans; C489347:N-isobutyl-2E-decenamide; D010936:Plant Extracts; D053284:Polyunsaturated Alkamides; C475934:hinokinin; D005680:gamma-Aminobutyric Acid; D008752:Methylene Chloride; D015107:4-Butyrolactone