title: Expression of interleukin-10 in intestinal lymphocytes detected by an interleukin-10 reporter knockin tiger mouse.
abstract: To identify interleukin-10 (IL-10)-producing cells in vivo, we generated a knockin mouse where an internal ribosome entry site (IRES) green fluorescence protein (GFP) element was inserted immediately before the polyadenylation site of the IL-10 gene. GFP fluorescence in cells from these mice was found to correlate positively with IL-10 protein expression. With this model, we found that after multiple T cell receptor (TCR) stimulations, strong expression of IL-10 was produced specifically by intraepithelial lymphocytes (IEL) in the small intestine and colonic lamina propria lymphocytes (cLPL). We found that anti-CD3 treatment induces T regulatory cell 1 (Tr1)-like cells in small intestinal IEL (sIEL) and led to the accumulation of naturally occurring regulatory T (nTreg) cells in colonic LPL (cLPL). These findings highlight the intestine as a unique site for induction of IL-10-producing T cells, which play a critical role in the regulation of inflammation in the gut.
authors.affiliation: Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
authors.forename: Masahito
authors.initials: M
authors.lastname: Kamanaka
authors.forename: Sean T
authors.initials: ST
authors.lastname: Kim
authors.forename: Yisong Y
authors.initials: YY
authors.lastname: Wan
authors.forename: Fayyaz S
authors.initials: FS
authors.lastname: Sutterwala
authors.forename: Maria
authors.initials: M
authors.lastname: Lara-Tejero
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Galán
authors.forename: Ed
authors.initials: E
authors.lastname: Harhaj
authors.forename: Richard A
authors.initials: RA
authors.lastname: Flavell
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000276:immunology / Q000378:metabolism; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D018928:Immunity, Mucosal; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000276:immunology; D007422:Intestines / Q000166:cytology / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology*; D051379:Mice; D008817:Mice, Mutant Strains; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D016753:Interleukin-10; D049452:Green Fluorescent Proteins