Bongkrekic acid and atractyloside inhibits chloride channels from mitochondrial membranes of rat heart.
The aim of this work was to characterize the effect of bongkrekic acid (BKA), atractyloside (ATR) and carboxyatractyloside (CAT) on single channel properties of chloride channels from mitochondria. Mitochondrial membranes isolated from a rat heart muscle were incorporated into a bilayer lipid membrane (BLM) and single chloride channel currents were measured in 250/50 mM KCl cis/trans solutions. BKA (1-100 microM), ATR and CAT (5-100 microM) inhibited the chloride channels in dose-dependent manner. The inhibitory effect of the BKA, ATR and CAT was pronounced from the trans side of a BLM and it increased with time and at negative voltages (trans-cis). These compounds did not influence the single channel amplitude, but decreased open dwell time of channels. The inhibitory effect of BKA, ATR and CAT on the mitochondrial chloride channel may help to explain some of their cellular and/or subcellular effects.
Malekova
Lubica
L
Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34 Bratislava, Slovak Republic.
Kominkova
Viera
V
Ferko
Miroslav
M
Stefanik
Peter
P
Krizanova
Olga
O
Ziegelhöffer
Attila
A
Szewczyk
Adam
A
Ondrias
Karol
K
D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D015153:Blotting, Western; D001865:Bongkrekic Acid / Q000378:metabolism* / Q000494:pharmacology; D018118:Chloride Channels / Q000037:antagonists & inhibitors*; D004305:Dose-Response Relationship, Drug; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D051336:Mitochondrial Membranes / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats
D018118:Chloride Channels; D001865:Bongkrekic Acid; D001278:Atractyloside; C003853:carboxyatractyloside