title: Agrobacterium-mediated transformation of yellow lupin to generate callus tissue producing HBV surface antigen in a long-term culture.
abstract: The idea of an oral vaccine administered as a portion of plant tissue requires a high level of antigen production. An improved protocol for the induction of transgenic yellow lupin calli or tumours, reaching 44% of transformation rate, is presented here. It has been developed by using the nptII marker gene and the uidA reporter gene as well as various Agrobacterium strains and plant explants. This method of seedling and hypocotyl transformation was applied to raise calli or tumours producing a small surface antigen of Hepatitis B Virus (S-HBsAg). Lupin tissue lines were long-term cultured on selection media maintaining the growth rate and high expression level of the native form of S-HBs, up to 6 microg per g of fresh tissue.
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland. tpni@igr.poznan.pl
authors.forename: Tomasz
authors.initials: T
authors.lastname: Pniewski
authors.forename: Józef
authors.initials: J
authors.lastname: Kapusta
authors.forename: Andrzej
authors.initials: A
authors.lastname: Płucienniczak
mesh_terms: D049109:Cell Proliferation; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis* / Q000235:genetics; D031297:Lupinus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified; D012231:Rhizobium / Q000235:genetics* / Q000378:metabolism; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic
chemical_list: D006514:Hepatitis B Surface Antigens