title: Delivery after transfer of frozen-thawed embryos from in vitro-matured oocytes in a woman at risk for ovarian hyperstimulation syndrome.
abstract: The present report describes the birth of a healthy infant after cryopreservation of embryos produced from in vitro-matured oocytes retrieved from a woman at risk of developing ovarian hyperstimulation syndrome (OHSS) during conventional in vitro fertilization (IVF) cycles. A conventional long protocol including gonadotropin-releasing hormone agonist (GnRHa) and gonadotropins induced a risk of OHSS. Oocyte retrieval was performed on day 11 of the cycle, and 27 immature oocytes were obtained. Following incubation for 24 h in maturation medium, 74.1% (20/27) of the oocytes were at the metaphase II stage. Fourteen oocytes (14/20, 70.0%) were fertilized after intracytoplasmic sperm injection (ICSI) with her husband's spermatozoa and cultured for 3 days. On day 4 following oocyte retrieval, three embryos at the 8-16 cell stage were transferred into the woman's uterus, and five spare embryos were frozen. Since the fresh embryo transfer failed to result in pregnancy, three post-thaw embryos were transferred into the woman three months later. Transfer of the frozen embryos resulted in pregnancy with delivery of a healthy infant girl.
authors.affiliation: Jiangsu Province Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Qian
authors.forename: Ting
authors.initials: T
authors.lastname: Feng
authors.forename: Ling-Bo
authors.initials: LB
authors.lastname: Cai
authors.forename: Jia-Yin
authors.initials: JY
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Ding
authors.forename: Yun-Dong
authors.initials: YD
authors.lastname: Mao
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D015925:Cryopreservation; D036861:Delivery, Obstetric; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D006801:Humans; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D016471:Ovarian Hyperstimulation Syndrome / Q000209:etiology; D011247:Pregnancy; D012307:Risk Factors