title: Pyrosequencing for rapid identification of carbapenem-hydrolysing OXA-type beta-lactamases in Acinetobacter baumannii.
abstract: Carbapenem-resistant Acinetobacter baumannii isolates producing carbapenem-hydrolysing oxacillinases are emerging worldwide. These enzymes are divided into four phylogenetic subgroups: OXA-23-like, OXA-51-like, OXA-24-like and OXA-58-like. A PCR-based approach linked to pyrosequencing analysis was developed to identify the genes for these beta-lactamases. Carbapenem-hydrolysing oxacillinases were rapidly and unambiguously identified in a collection of carbapenem-resistant clinical isolates of A. baumannii and Acinetobacter junii. Pyrosequencing may provide a rapid tool for identification of OXA variants, thus avoiding delays inherent in classical sequencing methods.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, K.-Bicêtre, France. thierry.naas@bct.ap-hop-paris.fr
authors.forename: T
authors.initials: T
authors.lastname: Naas
authors.forename: L
authors.initials: L
authors.lastname: Poirel
authors.forename: P
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000150:Acinetobacter / Q000201:enzymology / Q000235:genetics; D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000201:enzymology* / Q000235:genetics; D015780:Carbapenems / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D015780:Carbapenems; D017931:DNA Primers; D004269:DNA, Bacterial; D001618:beta-Lactamases; C108449:oxacillinase