title: Influence of mycoplasma contamination on the concentration and composition of extracellular RNA.
abstract: Kinetics of extracellular RNA accumulation in culture medium and at the cell surface along with their composition and distribution among cell-free and cell-surface-bound fractions were investigated in mycoplasma-contaminated and mycoplasma-free HeLa cells. It was shown that the mycoplasma infection influenced the concentration and kinetics of accumulation of total extracellular RNA and the distribution of specific RNA fragments among cell-free and cell-surface-bound fractions. Fragments of immature rRNA were found in culture of mycoplasma-infected HeLa cells. The data obtained indicate the existence of selective mechanisms providing binding of RNA with cell surface and their excretion out of cells.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, 8 Lavrentiev Ave., Novosibirsk 630090, Russia.
authors.forename: Evgeniy S
authors.initials: ES
authors.lastname: Morozkin
authors.forename: Elena Y
authors.initials: EY
authors.lastname: Rykova
authors.forename: Valentin V
authors.initials: VV
authors.lastname: Vlassov
authors.forename: Pavel P
authors.initials: PP
authors.lastname: Laktionov
mesh_terms: D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D009174:Mycoplasma / Q000378:metabolism*; D009175:Mycoplasma Infections; D012313:RNA / Q000737:chemistry / Q000378:metabolism
chemical_list: D012313:RNA