pEGFP-T, a novel T-vector for the direct, unidirectional cloning and analysis of PCR-amplified promoters.
A novel T-vector, designated as pEGFP-T, was constructed which could be used for direct and unidirectional cloning and analysis of promoters. The method involves minimizing the non-recombinant background of the T-vector when used to clone PCR products, and maintaining only the forward orientation of the PCR products prior to transformation. The usefulness of this vector is demonstrated in cloning and analyzing promoters.
Wang
Baoli
B
Key Research Lab of Hormone and Development Affiliated to the Public Health Ministry, Institute of Endocrinology, Tianjin Medical University, Tianjin, PR China. zhe0412@yahoo.com.cn.
Li
Xiaoxia
X
Zheng
Fang
F
Liu
Rui
R
Quan
Jinxing
J
Liang
Hui
H
Guo
Shanyi
S
Guo
Gang
G
Zhang
Jingyu
J
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic / Q000235:genetics*
D049452:Green Fluorescent Proteins