Antibody production with yeasts and filamentous fungi: on the road to large scale?
Yeasts and filamentous fungi have gained significant interest for the production of recombinant antibodies and antibody fragments. The opportunities and constraints of antibody (fragment) production in these hosts are highlighted as well as cell engineering strategies to overcome the constraints. Following aspects are addressed: folding, assembly and secretion of antibody related proteins, process optimization to improve productivity and quality, proteolysis, and, as a major point of interest, glycosylation.
Gasser
Brigitte
B
Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria.
Mattanovich
Diethard
D
D000906:Antibodies / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005658:Fungi / Q000378:metabolism*; D007136:Immunoglobulins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D015003:Yeasts / Q000378:metabolism*
D000906:Antibodies; D007136:Immunoglobulins; D011994:Recombinant Proteins