Evaluation of wound healing effect on skin-defect nude mice by using human dermis-derived mesenchymal stem cells.
We report that human dermis-derived mesenchymal stem cells (hDMSCs) possess differentiation potential of epidermis facilitating wound healing in skin-defect nude mice in combination with the treatment using gelatin/thermosensitive poly N-isopropylacrylamide (pNIPAAm)/polypropylene (PP). The results showed that the rate of cell growth and wound recovery in the hDMSC and gelatin/pNIPAAm/PP-treated group was significantly greater than those in the gelatin/pNIPAAm/PP-treated only group (P < .01). The reepithelialization marker of human pan-cytokeratin was also significantly increased on days 14 and day 21 in the wound site of hDMSCs and gelatin/pNIPAAm/PP-treated group. Furthermore, the stem cell marker of human CD13 gradually decreased during the period of wound healing. In sum, this novel method provided a transferring system for stem cell therapy, maintaining its temperature-sensitive property of easy peeling by lower temperature treatment.
Perng
C-K
CK
Department of Surgery, Division of Plastic and Reconstructive Surgery, Tatung University Taiwan, Taipei, Taiwan.
Ku
H-H
HH
Chiou
S-H
SH
Chen
I-L
IL
Tsai
F-T
FT
Yang
Y-P
YP
Chang
K Y
KY
Kao
C-L
CL
D000818:Animals; D020405:Dermis / Q000166:cytology; D004195:Disease Models, Animal; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008819:Mice, Nude; D012867:Skin / Q000473:pathology*; D014183:Transplantation, Heterologous; D014945:Wound Healing / Q000502:physiology*