Heat stress induced apoptosis in BC-8 cells derived from AK-5 tumor involves downregulation of Bcl-2 and generation of reactive oxygen species.
BC-8, a rat histiocytoma undergoes apoptosis after heat shock, which is due to lack of an effective heat shock response. Heat shock induced generation of free radicals, which in turn are involved in the induction of apoptotic death in BC-8 cells. Treatment of BC-8 cells with N-acetylcysteine partially inhibited the heat induced apoptosis. Introduction of Bcl-2 gene in these cells did not protect them from apoptotic death, whereas transfection with hsp-70 gene did render these cells resistant to heat induced apoptosis transiently. Heat shock also downregulated the expression of Bcl-2 and p53 in these cells. These observations suggested that the heat shock induced apoptosis was mediated through reactive oxygen species and controlled upstream of Bcl-2 check point.
Khar
Ashok
A
Centre for Cellular & Molecular Biology, Uppal Road, Hyderabad 500 007, India. khar@ccmb.res.in
Kumari
A Leela
AL
Pardhasaradhi
B V V
BV
Varalakshmi
Ch
Ch
Rangaraj
Nandini
N
D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D016166:Free Radical Scavengers / Q000494:pharmacology; D019254:Genes, bcl-2; D016158:Genes, p53; D005978:Glutathione / Q000378:metabolism; D018869:Heat-Shock Response; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*
D017304:Annexin A5; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D005978:Glutathione; D000111:Acetylcysteine