Growth kinetics and Pho84 phosphate transporter activity of Saccharomyces cerevisiae under phosphate-limited conditions.
The effect of phosphate (P ( i )) concentration on the growth behavior of Saccharomyces cerevisiae strain CEN.PK113-5D in phosphate-limited batch and chemostat cultures was studied. The range of dilution rates used in the present study was 0.08-0.45 h(-1). The batch growth of yeast cells followed Monod relationship, but growth of the cells in phosphate-limited chemostat showed change in growth kinetics with increasing dilution rates. The difference in growth kinetics of the yeast cells in phosphate-limited chemostat for dilution rates below and above approximately 0.2 h(-1) has been discussed in terms of the batch growth kinetic data and the change in the metabolic activity of the yeast cells. Immunological detection of a C-terminally myc epitope-tagged Pho84 fusion protein indicated derepressive expression of the Pho84 high-affinity P ( i ) transporter in the entire range of dilution rates employed in this study. Phosphate transport activity mediated by Pho84 transporter was highest at very low dilution rates, i.e. 0.08-0.1 h(-1), corresponding to conditions in which the amount of synthesized Pho84 was at its maximum.
Shokrollahzadeh
Soheila
S
Institute of Chemical Technologies, Iranian Research Organization for Science and Technology, Tehran, Iran. soheila.shokrollahzadeh@irost.com
Bonakdarpour
Babak
B
Vahabzadeh
Farzaneh
F
Sanati
Mehri
M
D018929:Cell Culture Techniques; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D010710:Phosphates / Q000032:analysis / Q000494:pharmacology*; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D028081:Proton-Phosphate Symporters / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics
D003470:Culture Media; C105495:PHO84 protein, S cerevisiae; D010710:Phosphates; D016271:Proto-Oncogene Proteins c-myc; D028081:Proton-Phosphate Symporters; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins