title: The use of transcriptional profiles to predict adult mosquito age under field conditions.
abstract: Age is a critical determinant of an adult female mosquito's ability to transmit a range of human pathogens. Despite its central importance, relatively few methods exist with which to accurately determine chronological age of field-caught mosquitoes. This fact is a major constraint on our ability to fully understand the relative importance of vector longevity to disease transmission in different ecological contexts. It also limits our ability to evaluate novel disease control strategies that specifically target mosquito longevity. We report the development of a transcriptional profiling approach to determine age of adult female Aedes aegypti under field conditions. We demonstrate that this approach surpasses current cuticular hydrocarbon methods for both accuracy of predicted age as well as the upper limits at which age can be reliably predicted. The method is based on genes that display age-dependent expression in a range of dipteran insects and, as such, is likely to be broadly applicable to other disease vectors.
authors.affiliation: School of Integrative Biology, University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Peter E
authors.initials: PE
authors.lastname: Cook
authors.forename: Leon E
authors.initials: LE
authors.lastname: Hugo
authors.forename: Iñaki
authors.initials: I
authors.lastname: Iturbe-Ormaetxe
authors.forename: Craig R
authors.initials: CR
authors.lastname: Williams
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Chenoweth
authors.forename: Scott A
authors.initials: SA
authors.lastname: Ritchie
authors.forename: Peter A
authors.initials: PA
authors.lastname: Ryan
authors.forename: Brian H
authors.initials: BH
authors.lastname: Kay
authors.forename: Mark W
authors.initials: MW
authors.lastname: Blows
authors.forename: Scott L
authors.initials: SL
authors.lastname: O'Neill
mesh_terms: D000375:Aging / Q000235:genetics*; D000818:Animals; D009033:Culicidae / Q000235:genetics*; D020869:Gene Expression Profiling; D014158:Transcription, Genetic / Q000235:genetics*