Proteome profiles of mucosal immunoglobulin uptake in inflamed porcine gut.
Acquisition of passive immunity by endocytosis of intact immunoglobulins (Ig) from colostrum is critical for prevention of intestinal and systemic diseases in neonatal mammals. We compared proteome patterns of healthy and inflamed gut tissues from pre-term piglets to investigate the effect of inflammation on acquisition of passive immunity. A clear difference in the two-dimensional gel electrophoresis protein patterns between healthy and inflamed intestinal tissues was observed, suggesting that inflamed tissues failed to absorb and transfer Ig from colostrum to epithelial cells. We have mapped and identified the Ig proteins that are taken up by healthy intestinal tissues, and found that isoforms of the IgA and IgG heavy chain and Ig kappa and lambda light chains were internalized. Our results indicate that colostrum protein uptake in the porcine gut is a selective process that is obstructed in inflamed pre-term gut.
Danielsen
Marianne
M
Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, Tjele, Denmark.
Thymann
Thomas
T
Jensen
Bent Borg
BB
Jensen
Ole Nørregaard
ON
Sangild
Per Torp
PT
Bendixen
Emøke
E
D000818:Animals; D000831:Animals, Newborn; D003126:Colostrum / Q000276:immunology / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020345:Enterocolitis, Necrotizing / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D007136:Immunoglobulins / Q000097:blood / Q000378:metabolism*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D040081:Protein Array Analysis; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D034421:Sus scrofa
D007136:Immunoglobulins; D020543:Proteome