Antibody-conjugated soybean oil-filled calcium phosphate nanoshells for targetted delivery of hydrophobic molecules.
Hollow calcium phosphate nanoparticles capable of encapsulating poorly water-soluble molecules were produced by self-assembly. Previously reported were solid calcium phosphate nanoparticles and water-filled calcium phosphate nanocapsules suited for encapsulating mostly hydrophilic, but not hydrophobic compounds. Here, calcium phosphate was deposited around 100 nm diameter, 1,2-dioleoyl-sn-glycero-3-phosphate stabilized soybean oil nanoemulsions using either calcium chloride or NaOH titrations to achieve shell thickness between 20-70 nm. The surface was functionalized with carboxylic acid via the addition of carboxyethylphosphonic acid to attach Molecular Probes AB-594C antibody using sulpho-n-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride with an efficiency of approximately 70%, while retaining near complete antibody function. Hydrophobic pyrene was encapsulated with an efficiency of 95%, at concentrations much higher than its water solubility limit, and exhibited spectral features characteristic of a hydrophobic environment. These materials can be used in the targeted delivery of many useful, yet poorly water-soluble pharmaceutical and nutraceutical compounds.
Schmidt
H T
HT
Chemical and Biomolecular Engineering and Center for Molecularly Engineered Materials, University of Notre Dame, Notre Dame, IN 46556, USA.
Kroczynski
M
M
Maddox
J
J
Chen
Y
Y
Josephs
R
R
Ostafin
A E
AE
D000906:Antibodies / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002122:Calcium Chloride / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D004396:Coloring Agents; D003692:Delayed-Action Preparations / Q000008:administration & dosage; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives / Q000737:chemistry; D004339:Drug Compounding / Q000379:methods; D004655:Emulsions / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron / Q000379:methods; D053761:Nanocomposites / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D010316:Particle Size; D012972:Sodium Hydroxide / Q000737:chemistry; D012995:Solubility; D013024:Soybean Oil
D000906:Antibodies; D000911:Antibodies, Monoclonal; D002130:Calcium Phosphates; D004396:Coloring Agents; D003692:Delayed-Action Preparations; D004655:Emulsions; D009943:Organophosphorus Compounds; D012972:Sodium Hydroxide; C003128:beta-phosphonopropionic acid; D004295:Dihydroxyphenylalanine; D013024:Soybean Oil; C059246:DOPA 3-phosphate; C020243:calcium phosphate; D002122:Calcium Chloride