Expression of ephrin in retinal neovascularization and iris rubeosis.
We investigated expression of ephrin-B2 and Eph-B4 in the retinal tissues of six primate eyes with neovascularization and iris rubeosis secondary to laser-induced central retinal vein occlusion and in tissue from 10 human eyes with proliferative diabetic retinopathy. Two primate eyes with rubeosis and retinal neovascularization were enucleated 1, 2 and 4 weeks after the creation of central retinal vein occlusion. Antibodies were localized using the avidin-biotin reaction. In the primate eyes, ephrin-B2 was negative at I week and positive at 2 and 4 weeks in the rubeotic tissue, but was positive only at 2 weeks in the retinal neovascular membrane. Eph-B4 was negative in all the primate eye specimens. In the human tissue, ephrin-B2 was detected in two of the five eyes with rubeosis and three of the five eyes with retinal neovascularization. These data suggest that ephrin-B2 is a key regulator of neovascularization.
Yuuki
T
T
Department of Ophthalmology, Gunma University School of Medicine, Gunma, Japan. ta.yuuki555@jcom.home.ne.jp
Kanda
T
T
Kishi
S
S
D042501:Angiogenic Proteins / Q000032:analysis; D000818:Animals; D003930:Diabetic Retinopathy; D004195:Disease Models, Animal; D036388:Ephrin-B2 / Q000032:analysis* / Q000235:genetics / Q000502:physiology*; D036342:Ephrins / Q000032:analysis / Q000235:genetics / Q000502:physiology; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D007499:Iris Diseases / Q000473:pathology; D008251:Macaca; D009389:Neovascularization, Pathologic / Q000473:pathology; D036224:Receptor, EphB4 / Q000032:analysis; D015356:Retinal Artery Occlusion; D015861:Retinal Neovascularization / Q000209:etiology / Q000473:pathology*; D013997:Time Factors
D042501:Angiogenic Proteins; D036388:Ephrin-B2; D036342:Ephrins; D036224:Receptor, EphB4