Utilization of hydrophobic bacterium Rhodococcus opacus B-4 as whole-cell catalyst in anhydrous organic solvents.
Rhodococcus opacus strain B-4, which has recently been isolated as an organic solvent-tolerant bacterium, has a high hydrophobicity and exhibits a high affinity for hydrocarbons. This bacterium was able to survive for at least 5 days in organic solvents, including n-tetradecane, oleyl alcohol, and bis(2-ethylhexyl) phthalate (BEHP), which contained water less than 1% (w/v). The biocatalytic ability of R. opacus B-4 was demonstrated in the essentially nonaqueous BEHP using indigo production from indole as a model conversion. By the catabolism of oleic acid for NADH regeneration, indigo production increased up to 71.6 microg ml(-1) by 24 h.
Yamashita
Shiho
S
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, Japan.
Satoi
Masafumi
M
Iwasa
Yoshihiro
Y
Honda
Kohsuke
K
Sameshima
Yuka
Y
Omasa
Takeshi
T
Kato
Junichi
J
Ohtake
Hisao
H
D000473:Alkanes / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D004051:Diethylhexyl Phthalate / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D005233:Fatty Alcohols / Q000494:pharmacology; D005285:Fermentation; D007203:Indigo Carmine; D007211:Indoles / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D050296:Microbial Viability; D009243:NAD / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism; D012240:Rhodococcus / Q000187:drug effects* / Q000378:metabolism; D012997:Solvents / Q000494:pharmacology*
D000473:Alkanes; D000900:Anti-Bacterial Agents; D003470:Culture Media; D005233:Fatty Alcohols; D007211:Indoles; D012997:Solvents; C024713:n-tetradecane; D009243:NAD; C010268:oleyl alcohol; D019301:Oleic Acid; C030374:indole; D004051:Diethylhexyl Phthalate; D007203:Indigo Carmine