Electrokinetic migration of acidic drugs across a supported liquid membrane.
Electrokinetic cross membrane extraction of acidic drugs was demonstrated for the first time. The acidic drugs were extracted from an alkaline aqueous donor solution (300 microl), through a thin supported liquid membrane of 1-heptanol sustained in the pores of the wall of a porous hollow fiber, and into an aqueous alkaline acceptor solution (30 microl) present inside the lumen of the hollow fiber by the application of a d.c. electrical potential. The negative electrode was placed in the donor solution, and the positive electrode was placed in the acceptor solution. Optimal extractions were accomplished with 1-heptanol as the supported liquid membrane, with 50 V as the driving force, and with pH 12.0 in both the donor and acceptor solutions, respectively (NaOH). Equilibrium extraction conditions were obtained after 5 min of operation with the whole assembly agitated at 1200 rpm. Eleven different acidic drugs were extracted with recovery values between 8 and 100%, and initial data supported that electrokinetic cross membrane extraction provided repeatable data and linear response between original donor concentration and final acceptor concentration of the acidic model compounds.
Balchen
Marte
M
School of Pharmacy, University of Oslo, Blindern, 0316 Oslo, Norway.
Gjelstad
Astrid
A
Rasmussen
Knut Einar
KE
Pedersen-Bjergaard
Stig
S
D000143:Acids / Q000302:isolation & purification*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary; D005480:Flurbiprofen / Q000302:isolation & purification; D015248:Gemfibrozil / Q000302:isolation & purification; D019850:Heptanol / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007660:Ketoprofen / Q000302:isolation & purification; D008567:Membranes, Artificial; D009288:Naproxen / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D011339:Probenecid / Q000302:isolation & purification; D015203:Reproducibility of Results; D014859:Warfarin / Q000302:isolation & purification
D000143:Acids; D008567:Membranes, Artificial; D004364:Pharmaceutical Preparations; D009288:Naproxen; D005480:Flurbiprofen; D014859:Warfarin; D019850:Heptanol; D007660:Ketoprofen; D011339:Probenecid; D015248:Gemfibrozil