Plasma volume expansion of 5% albumin, 4% gelatin, 6% HES 130/0.4, and normal saline under increased microvascular permeability in the rat.
OBJECTIVE
To compare the colloids 5% albumin, 4% gelatin, and 6% HES 130/0.4 with one another and with normal saline regarding their plasma expanding effects at increased permeability and to compare the results with those from a previous study at normal permeability.


METHODS
Prospective controlled randomized laboratory study in a university research laboratory.


METHODS
48 adult male Sprague-Dawley rats.


METHODS
Permeability was increased by an injection of 0.5 ml dextran 70 using the fact that dextran causes anaphylactic reaction in the rat. Plasma volume was determined ((125)I albumin tracer technique) after anesthesia, 1 h after dextran injection (before infusion for 10-15 min of 20 ml/kg bw of each of the colloids or 80 ml/kg saline), and 3 h later. Blood pressure, hematocrit, blood gases, and electrolytes were measured. CVP was measured in four rats.


RESULTS
Plasma volume was 41.1+/-1.9 ml/kg at baseline (n=9), and 29.1+/-4.1 ml/kg (n=35) 1 h after the dextran injection. Three hours after infusion of the plasma expander plasma volume had increased by 17.1+/-3.4 ml/kg in the albumin group, 7.9+/-3.6 ml/kg in the gelatin group, 7.4+/-4.4 ml/kg in the HES group, and 12.2+/-3.1 ml/kg in the saline group. It was unchanged in a control group given no solution (n=7 for all groups).


CONCLUSIONS
Albumin was a more effective plasma volume expander than gelatin or HES or saline (saline in 4 times larger volume). Gelatin and HES were equally effective. All solutions showed a smaller plasma expanding effect than observed in a previous study with normal permeability.
Dubniks
Maris
M
Department of Anesthesiology and Intensive Care, Lund University Hospital, 22185, Lund, Sweden.
Persson
Johan
J
Grände
Per-Olof
PO
D000418:Albumins / Q000494:pharmacology*; D000818:Animals; D002199:Capillary Permeability; D005780:Gelatin / Q000494:pharmacology*; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology*; D008297:Male; D010952:Plasma Substitutes / Q000494:pharmacology*; D010953:Plasma Volume / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000418:Albumins; C485123:HES 130-0.4; D006895:Hydroxyethyl Starch Derivatives; D010952:Plasma Substitutes; D005780:Gelatin