Cinnamtannin B-1 from bay wood reduces abnormal intracellular Ca2+ homeostasis and platelet hyperaggregability in type 2 diabetes mellitus patients.
Type 2 diabetes mellitus induces a number of cardiovascular disorders, including platelet hyperactivity and hyperaggregability, which is associated to an increased oxidant production and abnormal cytosolic Ca2+ mobilization. In the present study, we have investigated the effect of cinnamtannin B-1 obtained from bay wood on oxidants production, Ca2+ mobilization and aggregation in platelets from type 2 diabetic donors. Pretreatment of platelets with cinnamtannin B-1 reversed the enhanced oxidants production and Ca2+ mobilization, including Ca2+ entry, evoked by thapsigargin plus ionomycin or thrombin, observed in platelets from diabetic subjects, so that in the presence of cinnamtannin B-1 Ca2+ entry was similar in platelets from healthy and diabetic subjects. In addition, cinnamtannin B-1 reduced thrombin-induced aggregation in platelets from type 2 diabetic subjects. We conclude that cinnamtannin B-1 exerts an effective antioxidant action in platelets from patients with type 2 diabetes mellitus and reverses the enhanced Ca2+ mobilization and hyperaggregability.
Bouaziz
A
A
Unité de Recherche de Biochimie, Institut Supérieur de Biotechnologie, 5019 Monastir, Tunisia.
Salido
S
S
Linares-Palomino
P J
PJ
Sanchez
A
A
Altarejos
J
J
Bartegi
A
A
Salido
Ginés M
GM
Rosado
Juan A
JA
D000872:Anthocyanins / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002318:Cardiovascular Diseases / Q000209:etiology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications; D006706:Homeostasis; D006801:Humans; D066298:In Vitro Techniques; D027442:Laurus / Q000737:chemistry*; D028321:Plant Preparations / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D044945:Proanthocyanidins; D017382:Reactive Oxygen Species / Q000378:metabolism
D000872:Anthocyanins; D000975:Antioxidants; D028321:Plant Preparations; D044945:Proanthocyanidins; D017382:Reactive Oxygen Species; C517872:cinnamtannin B-1; D002118:Calcium