Determination of propoxyphene in oral fluid.
The determination of propoxyphene in oral fluid using solid-phase extraction and gas chromatography-mass spectrometry is described for the first time. The method employs collection of oral fluid with the Quantisal device, immunoassay screening of the specimen, confirmation of the positive screened samples after extraction using cation exchange/hydrophobic solid-phase extraction columns, optimized derivative formation, and gas chromatography-mass spectrometry in electron impact mode. Validated parameters including selectivity, linearity, accuracy, intra- and interday precision, extraction efficiency, and limit of quantitation were all within acceptable limits. The method was applied to authentic specimens taken from an individual prescribed propoxyphene following surgery.
Rana
Sumandeep
S
Immunalysis Corporation, 829 Towne Center Drive, Pomona, California 91767, USA.
Moore
Christine
C
Vincent
Michael
M
Coulter
Cynthia
C
Agrawal
Alpana
A
Soares
James
J
D000328:Adult; D011431:Dextropropoxyphene / Q000032:analysis* / Q000276:immunology; D005260:Female; D053593:Forensic Toxicology / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007118:Immunoassay; D009294:Narcotics / Q000032:analysis* / Q000276:immunology; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry* / Q000276:immunology; D012680:Sensitivity and Specificity
D009294:Narcotics; D011431:Dextropropoxyphene