title: Survivin and Cyclooxygenase-2 are co-expressed in human and mouse colon carcinoma and in terminally differentiated colonocytes.
abstract: In the evolution of colon rectal cancer (CRC) the imbalance between cell proliferation and apoptosis is considered one of the prominent causes of tumor induction and/or progression. In order to establish the role of anti apoptotic proteins in colon cancer development, we studied with immunohistochemical techniques the expression of Survivin in a mouse model of colon carcinogenesis induced by 1,2-dimethyl-hydrazine treatment. In this mouse model Survivin was over-expressed during tumor development, showing a distribution mimicking that described in the correspondent human malignancies. We also correlated Survivin distribution with COX-2 and beta-Catenin expression patterns. The co-localization of COX-2/beta-Catenin/Survivin in the same epithelial cells in tumor samples lends credence to possible in vivo regulatory effects of COX-2 and beta-Catenin on the intracellular Survivin levels in mouse and human colon cancer.
authors.affiliation: Biochemistry Department, Istituto di Ricerche di Biologia Molecolare I.R.B.M. Merck Research Laboratories, Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Mori
authors.forename: F R
authors.initials: FR
authors.lastname: Piro
authors.forename: C
authors.initials: C
authors.lastname: Della Rocca
authors.forename: G
authors.initials: G
authors.lastname: Mesiti
authors.forename: S
authors.initials: S
authors.lastname: Giampaoli
authors.forename: G
authors.initials: G
authors.lastname: Silvestre
authors.forename: D
authors.initials: D
authors.lastname: Lazzaro
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation; D003106:Colon / Q000378:metabolism / Q000473:pathology*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000166:cytology; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D051034:Inhibitor of Apoptosis Proteins; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D000077022:Survivin; D014018:Tissue Distribution; D051176:beta Catenin / Q000096:biosynthesis
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D000077022:Survivin; D051176:beta Catenin; D051546:Cyclooxygenase 2; D019813:1,2-Dimethylhydrazine