title: Effect of selenium-deficient diet on tubular epithelium in normal rats.
abstract: Selenium (Se) deficiency reduces glutathione peroxidase (GPx) activity, resulting in increased oxidative stress. We examined how Se deficiency induces renal injury via oxidative stress over time during the Se-deficient period. Seventy-two male Wistar rats were divided into two groups and fed either a control or Se-deficient diet. Rats were sacrificed on weeks 1, 2, 4, 6, 9, and 12. Blood and urine samples were collected, and the kidneys were removed. Urinalysis was performed, and creatinine clearance (Ccr) was calculated. Expressions of cellular GPx (cGPx) and phospholipid hydroperoxidase GPx (PHGPx) mRNA and GPx activity were measured. Histology was evaluated by light microscopy with immunohistochemistry for 4-hydroxy-2-nonenal (HNE) and vimentin. The Se-deficient diet caused significant decreases in GPx activity and cGPx mRNA expression but no change in PHGPx mRNA, together with significant proteinuria and glucosuria and slight decline in Ccr. The Se-deficient diet induced calcification in the kidney and increased the distribution of HNE and vimentin immunostaining in proximal tubuli, particularly around the outer medulla stripe. However, the histological damage did not progress after 6 weeks of deficiency. Se deficiency induces proteinuria and glucosuria with renal calcification, which may be primarily induced by injury of proximal tubuli via oxidative stress.
authors.affiliation: Department of Pediatrics, Kochi Medical School, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, 783-8505, Japan. fujiedam@med.kochi-u.ac.jp
authors.forename: Mikiya
authors.initials: M
authors.lastname: Fujieda
authors.forename: Keishi
authors.initials: K
authors.lastname: Naruse
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hamauzu
authors.forename: Eriko
authors.initials: E
authors.lastname: Miyazaki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Riyo
authors.initials: R
authors.lastname: Enomoto
authors.forename: Eibai
authors.initials: E
authors.lastname: Lee
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Ohta
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Wakiguchi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Enza
mesh_terms: D000818:Animals; D003404:Creatinine / Q000652:urine; D004848:Epithelium / Q000473:pathology / Q000503:physiopathology; D005979:Glutathione Peroxidase / Q000378:metabolism; D006029:Glycosuria / Q000209:etiology / Q000378:metabolism; D007684:Kidney Tubules / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D018384:Oxidative Stress / Q000502:physiology; D011507:Proteinuria / Q000209:etiology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage / Q000172:deficiency* / Q000502:physiology
chemical_list: D003404:Creatinine; D005979:Glutathione Peroxidase; D012643:Selenium