[Selective determination for calf thymus DNA by a resonance light scattering technique with morin-Ce(IV) system].
The interaction of DNA and the binary complex formed between Ce(IV) and morin was studied. With the maximum scattering peak located at 320 nm, the enhanced RLS was proportional to the concentration of ctDNA in the range 0-25 microg x mL(-1) and the detection limit (3sigma) of 0.3 microg x mL(-1) was obtained. Under the optimized conditions, four synthetic samples were determined with recoveries in the range 93.7%-108.4%. The proposed method is particularly attractive for its high selectivity for ctDNA in the presence of hsDNA by using certain sensitive spectrofluorometer since the intensity of RLS for hsDNA is far weaker than that for ctDNA.
Zhou
Min
M
Institute of Chemistry, Northwest Normal University, Lanzhou 730070, China.
Ma
Yong-Jun
YJ
Zhang
De-Yi
DY
Chen
Hui
H
D000818:Animals; D002417:Cattle; D002563:Cerium / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry*; D008027:Light; D008297:Male; D009942:Organometallic Compounds / Q000737:chemistry; D009994:Osmolar Concentration; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012965:Sodium Chloride / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013464:Sulfuric Acids / Q000737:chemistry; D047309:Flavones
D002563:Cerium; D004247:DNA; D005419:Flavonoids; D009942:Organometallic Compounds; D012965:Sodium Chloride; D013464:Sulfuric Acids; C008548:morin; D047309:Flavones; C427203:calf thymus DNA; C033158:sulfuric acid