Identification and characterization of novel activity-dependent transcription factors in rat cortical neurons.
Using gene chip analyses, we have identified novel neuronal activity-dependent genes. Application of 25 mM KCl to mature (14-day culture) rat cortical neurons resulted in more than 1.5-fold induction of 19 genes and reduction of 42 genes among 1200 neural genes. Changes in the overall gene expression profiles appeared to be related to the reduction of excitability and induction of cellular survival signals. Among the genes identified, three transcriptional modulators [encoding Cbp/p300-interacting transactivator with ED-rich tail 2 (CITED2), CCAAT/enhancer binding protein beta (C/EBPbeta) and neuronal orphan receptor-1, (NOR1)] were newly identified as activity-dependent transcription factors, and two of these (CITED2 and NOR1) were found to be influenced by electroconvulsive shock (ECS). NOR1 was induced in specific brain regions by behavioral activation, such as exposure to a novel environment. Because the brain regions that exhibited the induction of these newly identified neuronal activity-dependent transcriptional modulators were distinct from those showing the induction of previously identified activity-dependent genes such as c-fos, these genes might be useful markers for mapping neuronal activity in vivo.
Sun
Woong
W
Department of Anatomy, College of Medicine, Brain Korea 21 Program, Korea University, Seoul, Korea.
Choi
Sang-Hyun
SH
Park
Soon Kwon
SK
Kim
Soon Jung
SJ
Noh
Mi Ra
MR
Kim
Eun Hae
EH
Kim
Hyun Ju
HJ
Kim
Hyun
H
D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004597:Electroshock / Q000379:methods; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011189:Potassium Chloride / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013223:Statistics as Topic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015533:Transcriptional Activation / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects
C517260:CITED2 protein, rat; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C091333:Nr4a3 protein, rat; D014157:Transcription Factors; D011189:Potassium Chloride