Development of a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys (Macaca fascicularis).
To develop a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys (Macaca fascicularis), 148 microsatellite markers were selected from the human genome database. The polymorphisms and inheritance of PCR products were determined by screening twenty unrelated monkeys and by analysis of three families, respectively. As a result, 106 primers (72%) gave PCR products of the size expected for humans and rhesus monkeys. Among these products, polymorphism and single-gene inheritance in cynomolgus monkeys was observed for 66 markers (62%). The average number of alleles at the 66 polymorphic loci was 5.86 (range 2-10), and average heterozygosity was 0.63 (range 0.10-0.88). This is the first report of microsatellite markers for cynomolgus monkeys. Chromosomal mapping of these markers is now in progress.
Kikuchi
Toshihiko
T
Tsukuba Primate Research Center, National Institute of Biomedical Innovation, 1-1 Hachimandai, Tskukuba, Ibaraki 305-0843, Japan.
Hara
Masayuki
M
Terao
Keiji
K
D000818:Animals; D017931:DNA Primers; D005260:Female; D005819:Genetic Markers / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods*; D016678:Genome / Q000235:genetics*; D040582:Inheritance Patterns / Q000235:genetics; D008252:Macaca fascicularis / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D017931:DNA Primers; D005819:Genetic Markers