title: Immunoblot analysis for IgE-reactive components of fetal calf serum in dogs that developed allergic reactions after non-rabies vaccination.
abstract: We previously demonstrated the presence of IgE directed to fetal calf serum (FCS) in the sera from dogs that developed allergic reactions after vaccination. In this study, by an immunoblot analysis, we investigated the IgE-reactive components of FCS using sera from 16 dogs that exhibited allergic reactions after vaccination. The immunoblot analysis revealed that several FCS proteins of approximately 25-, 50-, 66-, 75-, 120-, and 175-kDa strongly reacted with IgE in the sera from dogs that showed post-vaccination allergic reactions. The 66-kDa band was detected in the sera from 14 of the 16 dog serum samples analyzed in the immunoblot analysis for FCS, and it was speculated to be albumin based on its molecular weight; however, serum IgE reactivity to bovine serum albumin could be detected in only four of the 14 dog samples. These findings demonstrated that a variety of FCS components including albumin could function as allergens in dogs that developed allergic reactions after vaccination.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
authors.forename: Keitaro
authors.initials: K
authors.lastname: Ohmori
authors.forename: Kenichi
authors.initials: K
authors.lastname: Masuda
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: DeBoer
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000209:etiology*; D004285:Dogs; D005260:Female; D006967:Hypersensitivity / Q000209:etiology / Q000662:veterinary*; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D008970:Molecular Weight; D012710:Serum Albumin, Bovine / Q000009:adverse effects*; D014611:Vaccination / Q000009:adverse effects* / Q000662:veterinary*
chemical_list: D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E