title: Signature-tagged mutagenesis: barcoding mutants for genome-wide screens.
abstract: DNA signature tags (molecular barcodes) facilitate functional screens by identifying mutants in mixed populations that have a reduced or increased adaptation to a particular environment. Many innovative adaptations and refinements in the technology have been described since its original use with Salmonella; they have yielded a wealth of information on a broad range of biological processes--mainly in bacteria, but also in yeast and other fungi, viruses, parasites and, most recently, in mammalian cells. By combining whole-genome microarrays and comprehensive ordered libraries of mutants, high-throughput functional screens can now be achieved on a genomic scale.
authors.affiliation: Department of Infectious Diseases, Centre for Molecular Microbiology and Infection, Imperial College London, Armstrong Road, London SW7 2AZ, UK.
authors.forename: Piotr
authors.initials: P
authors.lastname: Mazurkiewicz
authors.forename: Christoph M
authors.initials: CM
authors.lastname: Tang
authors.forename: Charles
authors.initials: C
authors.lastname: Boone
authors.forename: David W
authors.initials: DW
authors.lastname: Holden
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000254:growth & development; D015723:Gene Library; D016678:Genome; D006801:Humans; D016254:Mutagenesis, Insertional; D009154:Mutation / Q000235:genetics*; D034622:RNA Interference; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development