[Occurrence of adhesin genes in coagulase-negative Staphylococcus aureus strains].
Sixty fenotypically coagulase-negative Staphylococcus aureus strains were screened for the presence of six adhesion genes. The strains were isolated from varied clinical samples and varied patients in 16 medical centers, in majority from the region of Gdansk. Multiplex PCR in two primer sets was used for detection of the following genes: bbp (bone binding protein), cna (collagen binding protein), ebp (elastin binding protein)and fnbB (fibronectin B binding protein), fib (fibrinogen bindng protein) and clfA (clunmping factor A). More than half (57%) of the examined population harbored four genes: fnbB,fib, cna i clfA. All of the strains were found to be clfA positive and 90% of them were positive for fnbB, 90% for fib and 67% for cna. All of these genes were significantly more common in MRSA than in MSSA. The particular genes were occurred in strains derived from varied clinical samples.
Wiśniewska
Katarzyna
K
Katedra Mikrobiologii, Zakład Mikrobiologii Lekarskiej AM w Gdańsku. kwis@amg.gda.pl
Garbacz
Katarzyna
K
Piechowicz
Lidia
L
D018829:Adhesins, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000302:isolation & purification; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003030:Coagulase / Q000378:metabolism*; D006801:Humans; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000378:metabolism*
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003030:Coagulase; C089677:Fib protein, Staphylococcus aureus; C048165:adhesin, Staphylococcus aureus