[Novel mutations of cathepsin C gene in two Chinese patients with Papillon-Lefèvre syndrome].
OBJECTIVE
To investigate the mutational characteristics of cathepsin C (CTSC) gene in two Chinese patients with Papillon-Lefèvre syndrome (PLS), and provide molecular basis for research of the pathogenesis of PLS.


METHODS
Peripheral blood samples were obtained from patients and their parents respectively. Genomic DNA were extracted after consents. Polymerase chain reaction, direct DNA sequencing and restriction enzyme reaction were performed to screen mutations of CTSC gene.


RESULTS
Compound heterozygous mutations of CTSC gene were identified in the two patients. Patient I carried the G139R and S260P mutations, patient II had the R250X and C258W mutations. The parents were heterozygous carriers without the clinical feature of PLS. None of the mutations were detected in normal controls. Furthermore, the S260P and C258W changes were novel mutations of CTSC gene, which had not been reported previously.


CONCLUSIONS
Mutations of CTSC gene are responsible for the phenotype of Papillon-Lefèvre syndrome in two Chinese patients. The results extend the mutation spectrum of CTSC gene and also provide basis for gene diagnosis of PLS in China.
Yang
Yuan
Y
Department of Pedodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Bai
Xiao-wen
XW
Liu
Hong-sheng
HS
Cao
Cai-fang
CF
Ge
Li-hong
LH
D044466:Asian People / Q000235:genetics; D020789:Cathepsin C / Q000235:genetics*; D002675:Child, Preschool; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D020125:Mutation, Missense / Q000235:genetics*; D010214:Papillon-Lefevre Disease / Q000201:enzymology / Q000235:genetics*
C533918:CTSC protein, human; D020789:Cathepsin C