Assay of human gastric mucin as a natural antibiotic against Helicobacter pylori.
Helicobacter pylori infects more than half of the world's population and is considered a leading cause of peptic ulcer and gastric carcinoma. Although a large number of persons are infected with H. pylori, only a limited number of those infected (approximately 3%) develop peptic ulcers and gastric carcinoma. The progression of the disease is restricted by deeper portion of the gastric mucosa, and in many persons glandular atrophy appears to be prevented by mucins secreted in the deeper portion of the mucosa. Recent studies have shown that this inhibitory activity is at least partly due to the expression of alpha1,4-N-acetylglucosamine residues attached to the mucin (MUC6) in the deeper portion of the mucosa. alpha1,4-N-acetylglucosamine residues inhibit cholesterol alpha-glucosyltransferase, the product of which constitutes a major component of H. pylori cell wall. This inhibitory activity is thus regarded as a natural antibiotic function. This chapter describes the assay for antibiotic activity of MUC6 mucin against H. pylori infection and growth as well as inhibition by alpha1,4-N-acetylglucosamine-capped mucin-type oligosaccharides.
Fukuda
Minoru
M
Glycobiology Program, Cancer Research Center, The Burnham Institute for Medical Research, La Jolla, CA, USA.
Kawakubo
Masatomo
M
Ito
Yuki
Y
Kobayashi
Motohiro
M
Lee
Heeseob
H
Nakayama
Jun
J
D000117:Acetylglucosamine / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002784:Cholesterol / Q000378:metabolism; D005752:Gastric Mucins / Q000378:metabolism*; D005753:Gastric Mucosa / Q000737:chemistry / Q000382:microbiology*; D005964:Glucosyltransferases / Q000737:chemistry / Q000378:metabolism; D016481:Helicobacter Infections / Q000378:metabolism; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D051917:Leukosialin / Q000378:metabolism; D008969:Molecular Sequence Data; D055272:Mucin-6; D009077:Mucins / Q000737:chemistry / Q000378:metabolism
D000900:Anti-Bacterial Agents; D005752:Gastric Mucins; D051917:Leukosialin; C524734:MUC6 protein, human; D055272:Mucin-6; D009077:Mucins; D002784:Cholesterol; D005964:Glucosyltransferases; D000117:Acetylglucosamine