title: [Preparation and testing of buffered eye drops containing pilocarpine chloride with timolol maleate].
abstract: OBJECTIVE
  In glaucoma therapy beta-blocator, timolol-maleate, is the first-line medicine of selection, whereas a miotic, pilocarpine chloride, is one of the oldest medicines used for the treatment of this illness. They are applied as eye drops. In order to achieve a better therapeutic effect and improve life quality of the ill, we produced and tested eye drops formulations based on combining pilocarpinechloride and timolol maleate in buffers of diferent pH values.
  
  
  METHODS
  Following the general pharmacopoeial eye drops preparation regulation, we prepared formulations, of solution type, of pilocarpine chloride and timolol maleate combination, of pilocarpine choloride alone and of timolol maleate alone. A modified phosphate buffer according to Sorensen at 7.4, 7.7 and 8.0 pH values was used as a solvent. The quality of the produced formulations was examined using physical and physico-chemical methods and biological tests. Following pharmacopoeial regulations, we examined clarity, pH value and sterilty. Pilocarpine chloride level was determined by means of ion-par (High Performance Liguid Chromatography) RP-HPLC method, whereas UV/VIS absorption spectrophotometry was used for determining the level of both timolol maleate, and timolol.
  
  
  RESULTS
  Results showed that monocomponent and combined preparations complied with the regulation demands. With the increase in the pH value of the solution pilocarpine chloride level decreased in relation to its initial content, whereas timolol level showed a tendency of moderate increase.
  
  
  CONCLUSIONS
  Magistrally prepared pilocarpine chloride eye drops with timolol maleate have satisfied all the required conditions for an ophtalmological preparation. A modified phosphate buffer according to Sorensen at 7.4 pH value proved to be the most optimal solvent.
authors.affiliation: Dom zdravlja Mladenovac, Apoteka ",Zdravlje", Mladenovac, Srbija.
authors.forename: Danijela
authors.initials: D
authors.lastname: Rakić
authors.forename: Mirjana
authors.initials: M
authors.lastname: Antunović
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000032:analysis*; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004339:Drug Compounding; D006863:Hydrogen-Ion Concentration; D008916:Miotics / Q000032:analysis*; D009883:Ophthalmic Solutions / Q000737:chemistry*; D010862:Pilocarpine / Q000032:analysis*; D013999:Timolol / Q000032:analysis*
chemical_list: D000319:Adrenergic beta-Antagonists; D002021:Buffers; D004338:Drug Combinations; D008916:Miotics; D009883:Ophthalmic Solutions; D010862:Pilocarpine; D013999:Timolol