title: Proteolytic activation of Vibrio mimicus (Vm) major outer membrane protein haemagglutinin (HA) with Vm-HA/protease: Implication for understanding bacterial adherence.
abstract: Vibrio mimicus (Vm) haemagglutinins (HAs), such as an extracellular HA/protease (Vm-HA/protease) and a major outer membrane protein-HA (Vm-OMPHA), have been recognized as the putative adherence factors for the bacterium. However, the mechanism by which HAs coordinate the adherence function of the bacterium remains as yet unknown. We report herein the positive interaction between Vm-HA/protease and Vm-OMPHA resulting in significant enhancement of the haemagglutinating ability. In this interaction, no cleaved polypeptide was detected; however, limited proteolysis of Vm-OMPHA was confirmed by SDS-PAGE. The proteolytic activation of the native cell-associated Vm-OMPHA by limited proteolysis was also demonstrated in several V. mimicus strains. Proteolytic activation of OMPHA was also achieved with various proteases from bacterial and eukaryotic sources. These findings may indicate a novel coordination of V. mimicus HAs in the adherence of the bacterium.
authors.affiliation: Laboratory of Enteric Microbiology, International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh. munirul@icddrb.org
authors.forename: Munirul
authors.initials: M
authors.lastname: Alam
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Miyoshi
authors.forename: Kabir Uddin
authors.initials: KU
authors.lastname: Ahmed
authors.forename: Nur A
authors.initials: NA
authors.lastname: Hasan
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Tomochika
authors.forename: Sumio
authors.initials: S
authors.lastname: Shinoda
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D004789:Enzyme Activation; D006388:Hemagglutinins / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000378:metabolism*; D044164:Vibrio mimicus / Q000378:metabolism* / Q000502:physiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D006388:Hemagglutinins; D008666:Metalloendopeptidases; C039814:hemagglutinin-protease