[Expression of rabbit neutrophile peptide-1 in nitrate reductase-deficient mutant of Chlorella ellipsoidea].
Application of transgenic Cholrella as bioreactor to express rabbit neutrophile pepetide-1 (NP-1) shows great practical value. In this paper, an NP-1 expression vector containing two selective marker genes NPTII and nitrate reductase gene was constructed. The NP-1 gene was transformed into the nitrate reductase-deficient mutant nrm-4 of Chlorella ellipsoidea via electroporation, and the transgenic alga expressed the active NP-1 were obtained.
Zhang
Xiao-Yu
XY
State Key Laboratory of Plant Genomics and National Plant Gene Research Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. xiaoyuzh8000@163.com
Wang
Peng
P
Zhao
Shi-Min
SM
Li
Xia
X
Shen
Xin
X
Sun
Yong-Ru
YR
Chu
Cheng-Cai
CC
Wang
Yi-Qin
YQ
D000818:Animals; D000898:Antibiosis; D019149:Bioreactors; D002708:Chlorella / Q000201:enzymology / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics; D018274:Electroporation; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D009154:Mutation; D050901:Nitrate Reductase / Q000172:deficiency* / Q000235:genetics; D030781:Organisms, Genetically Modified; D011817:Rabbits; D023084:alpha-Defensins / Q000096:biosynthesis* / Q000235:genetics*
D023084:alpha-Defensins; D050901:Nitrate Reductase