Multimodal image-guided enzyme/prodrug cancer therapy.
The conjugate of bacterial cytosine deaminase (bCD) and poly-l-lysine (PLL) that was functionalized with biotin, rhodamine, and Gd3+-DOTA was synthesized and characterized. It demonstrated high relaxivity, improved enzymatic specificity to prodrug 5-fluorocytosine, low cytotoxicity, efficient cell uptake, and high enzymatic stability in fresh mouse serum and human breast cancer cell culture.
Li
Cong
C
JHU ICMIC Program, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Winnard
Paul T
PT
Takagi
Tomoyo
T
Artemov
Dmitri
D
Bhujwalla
Zaver M
ZM
D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics / Q000628:therapy; D045744:Cell Line, Tumor; D043525:Cytosine Deaminase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D005437:Flucytosine / Q000493:pharmacokinetics* / Q000494:pharmacology; D006571:Heterocyclic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008027:Light; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011107:Polylysine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D011355:Prodrugs / Q000493:pharmacokinetics*; D012235:Rhodamines / Q000737:chemistry* / Q000378:metabolism; D012542:Scattering, Radiation
D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; D011355:Prodrugs; D012235:Rhodamines; C069063:polylysine-(Gd-DOTA); D011107:Polylysine; D005437:Flucytosine; D043525:Cytosine Deaminase