Time-resolved methods in Biophysics. 1. A novel pump and probe surface-enhanced resonance Raman approach for studying biological photoreceptors.
This article describes a method, based on surface-enhanced resonance Raman (SERR) spectroscopy, for studying the reaction dynamics of photoreceptors immobilized on metal electrodes. Time resolution and fresh sample conditions are achieved by synchronizing the rotational and translational motion of a novel kinematic electrode with the duration and time delay between the pump and probe events. The power and sensitivity of the method is illustrated by studying the photocycle of the sensory photoreceptor NpSRII and its sensitivity to the applied electric field. The results are compared with time-resolved resonance Raman measurements in solution.
Naumann
Hendrik
H
Technische Universität Berlin, Institut für Chemie, Max Volmer Laboratorium für Biophysikalische Chemie, Sekr. PC 14, Strasse des 17. Juni 135, D-10623, Berlin, Germany.
Klare
Johann P
JP
Engelhard
Martin
M
Hildebrandt
Peter
P
Murgida
Daniel H
DH
D001703:Biophysics / Q000379:methods*; D010786:Photoreceptor Cells / Q000378:metabolism*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods*; D013997:Time Factors