PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections.
We developed a real-time PCR which allowed the highly sensitive detection of Naegleria fowleri in histological brain tissue sections from experimentally infected mice. This genus-specific small-subunit (18S) rRNA gene-based PCR can complement conventional (immuno-) histology for the diagnosis of primary amoebic meningoencephalitis in paraffin-embedded brain necropsy specimens that had been fixed in formalin buffered with phosphate-buffered saline.
Schild
Marc
M
Institute of Parasitology, University of Berne, Länggass-Strasse 122, CH-3012 Bern, Switzerland.
Gianinazzi
Christian
C
Gottstein
Bruno
B
Müller
Norbert
N
D000562:Amebiasis / Q000175:diagnosis / Q000469:parasitology; D000818:Animals; D001921:Brain / Q000469:parasitology*; D016054:DNA, Protozoan / Q000032:analysis; D005404:Fixatives; D005557:Formaldehyde; D008590:Meningoencephalitis / Q000175:diagnosis* / Q000469:parasitology; D051379:Mice; D008810:Mice, Inbred C57BL; D016848:Naegleria fowleri / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D016612:Paraffin Embedding / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods*
D016054:DNA, Protozoan; D005404:Fixatives; D005557:Formaldehyde