Terbutaline inhibits corticotropin-releasing hormone (CRH) expression in human trophoblast cells.
OBJECTIVE
To evaluate the effect of beta-adrenergic agonists on the regulation of the expression of the placental corticotropin-releasing hormone (CRH) gene.


METHODS
Term placentae were collected at the time of elective cesarean section, and trophoblast cells were harvested, isolated, and cultured. The isolated trophoblasts were plated, cultured and subsequently treated with cortisol, terbutaline, RU486, or vehicle control. CRH expression, mRNA abundance of CRH, and the housekeeping gene beta-actin were evaluated by Northern blot analysis.


RESULTS
Exposure of the trophoblasts to terbutaline (10(-8) M) inhibited the expression of the CRH gene as depicted by Northern blot analysis. Co-addition of terbutaline (10(-8) M) and RU486 (10(-6) M) did not block the stimulatory effects of RU486 on placental trophoblast cells.


CONCLUSIONS
The beta-adrenergic agonist terbutaline inhibits the expression of CRH in human trophoblasts. This finding may provide insight into the mechanism of action of terbutaline as a tocolytic agent.
Kontopoulos
Eftichia V
EV
Department of Obstetrics, Gynecology and Reproductive Sciences, Robert Wood Johnson University Hospital/Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick, USA. ekontopo@hsc.usf.edu
Chandras
Christina
C
Karalis
Katia
K
D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D013726:Terbutaline / Q000494:pharmacology*; D014327:Trophoblasts / Q000378:metabolism*
D012333:RNA, Messenger; D003346:Corticotropin-Releasing Hormone; D013726:Terbutaline