title: Molecular cloning and characterization of mouse aquaporin 6.
abstract: In the rat kidney, aquaporin (AQP) 6 is localized in the intracellular vesicle membranes of type-A intercalated cells of the collecting duct; mouse AQP6 (mAQP6) has not been characterized. Although mAQP6 was originally cloned from cDNA in a mouse cerebellum library (GenBank NM 175087), we have independently cloned a cDNA encoding mAQP6 from an adult kidney cDNA library (C57BL/6J strain). We identified two different spliced variants of mAQP6: mAQP6a and mAQP6b. The mAQP6a isoform is almost identical to that of rat AQP6, whereas mAQP6b is identical to that reported in the mouse cerebellum library mentioned above. We found that the mRNA expression of these two spliced variants is regulated in a tissue-specific and age-dependent manner. Functional analyses of water and ion permeation revealed that mAQP6a functions like rat AQP6 and that mAQP6b does not function as either a water channel or an ion channel under our experimental conditions.
authors.affiliation: Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205-2185, USA.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nagase
authors.forename: Johan
authors.initials: J
authors.lastname: Agren
authors.forename: Akiko
authors.initials: A
authors.lastname: Saito
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.forename: Peter
authors.initials: P
authors.lastname: Agre
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hazama
authors.forename: Masato
authors.initials: M
authors.lastname: Yasui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D051403:Aquaporin 6 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D003001:Cloning, Molecular; D015870:Gene Expression; D007668:Kidney / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D051381:Rats; D016415:Sequence Alignment; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D051403:Aquaporin 6