Enhanced NTPDase and 5'-nucleotidase activities in diabetes mellitus and iron-overload model.
The activity of the enzymes NTPDase and 5'-nucleotidase was studied in both diabetes mellitus and an associated model of iron-overload. Rats were divided in five groups: citrate (CC), saline (S), diabetic (D), iron-overload (IO), and diabetic iron-overload (DIO). Diabetes was induced with alloxan (150 mg/kg), and iron-overload was induced with iron-dextran (10 intramuscular applications of +/-80 mg/kg). The enzymatic activities were evaluated in the platelets. The results demonstrated an increase in the activity of NTPDase with substrates ATP and ADP (60% and 120%, respectively; P<0.001), and 5'-nucleotidase (60%, P<0.001). This increase was more intense in the IO and DIO groups. The results obtained in vitro showed an activation in ATP, ADP, and AMP hydrolysis between 1 microM and 1,000 microM ferric nitrate concentrations, being more pronounced at 100 microM and decreasing at 1,000 microM. We concluded that diabetes mellitus in association with iron-overload increased the hydrolysis of adenine nucleotides in platelets, contributing to the abnormalities found in these pathological conditions.
Miron
Vinícius Ribas
VR
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brasil.
Bauermann
Liliane
L
Morsch
André Luis Bittencourt
AL
Zanin
Rafael Fernandes
RF
Corrêa
Maísa
M
da Silva
Adriane Cismoski
AC
Mazzanti
Cínthia
C
Morsch
Vera Maria
VM
Lunkes
Gilberto Ignácio
GI
Schetinger
Maria Rosa Chitolina
MR
D015720:5'-Nucleotidase / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000378:metabolism; D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D001081:Apyrase / Q000378:metabolism*; D001786:Blood Glucose / Q000032:analysis; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000201:enzymology*; D004195:Disease Models, Animal; D005290:Ferric Compounds / Q000494:pharmacology; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D006868:Hydrolysis / Q000187:drug effects; D007501:Iron / Q000097:blood; D019190:Iron Overload / Q000139:chemically induced / Q000201:enzymology*; D008297:Male; D009566:Nitrates / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000227:Adenine Nucleotides; D015703:Antigens, CD; D001786:Blood Glucose; D005290:Ferric Compounds; D006454:Hemoglobins; D009566:Nitrates; D007501:Iron; D015720:5'-Nucleotidase; D001081:Apyrase; C067869:CD39 antigen; C025302:ferric nitrate