Optimization of culture conditions and scale-up to pilot and plant scales for coenzyme Q10 production by Agrobacterium tumefaciens.
This report describes the optimization of culture conditions for coenzyme Q(10) (CoQ(10)) production by Agrobacterium tumefaciens KCCM 10413, an identified high-CoQ(10)-producing strain (Kim et al., Korean patent. 10-0458818, 2002b). Among the conditions tested, the pH and the dissolved oxygen (DO) levels were the key factors affecting CoQ(10) production. When the pH and DO levels were controlled at 7.0 and 0-10%, respectively, a dry cell weight (DCW) of 48.4 g l(-1) and a CoQ(10) production of 320 mg l(-1) were obtained after 96 h of batch culture, corresponding to a specific CoQ(10) content of 6.61 mg g-DCW(-1). In a fed-batch culture of sucrose, the DCW, specific CoQ(10) content, and CoQ(10) production increased to 53.6 g l(-1), 8.54 mg g-DCW(-1), and 458 mg l(-1), respectively. CoQ(10) production was scaled up from a laboratory scale (5-l fermentor) to a pilot scale (300 l) and a plant scale (5,000 l) using the impeller tip velocity (V (tip)) as a scale-up parameter. CoQ(10) production at the laboratory scale was similar to those at the pilot and plant scales. This is the first report of pilot- and plant-scale productions of CoQ(10) in A. tumefaciens.
Ha
Suk-Jin
SJ
BioNgene Co., Ltd., 10-1 Myungryun-Dong Chongro-Ku, Seoul 110-521, South Korea.
Kim
Sang-Yong
SY
Seo
Jin-Ho
JH
Oh
Deok-Kun
DK
Lee
Jung-Kul
JK
D016960:Agrobacterium tumefaciens / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D003067:Coenzymes / Q000096:biosynthesis; D046508:Culture Techniques / Q000379:methods; D007218:Industrial Microbiology; D009584:Nitrogen / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis
D003067:Coenzymes; D014451:Ubiquinone; D002244:Carbon; C024989:coenzyme Q10; D009584:Nitrogen