title: Microglial cell line established from prion protein-overexpressing mice is susceptible to various murine prion strains.
abstract: Several lines of evidence suggest that microglia have important roles in the pathogenesis of prion diseases. Here, we establish a novel microglial cell line (MG20) from neonatal tga20 mice that overexpress murine prion protein. After exposure to Chandler scrapie, we observed the replication and accumulation of disease-associated forms of the prion protein in MG20 cells up to the 15th passage. Furthermore, MG20 cells were susceptible to ME7, Obihiro scrapie, and bovine spongiform encephalopathy agents. Thus, MG20 cell lines persistently infected with various murine prion strains provide a useful model for the study of the pathogenesis of prion diseases.
authors.affiliation: Research Center for Prion Diseases, National Institute of Animal Health, Kannondai 3-1-5, Tsukuba, Ibaraki 305-8602, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Iwamaru
authors.forename: Takato
authors.initials: T
authors.lastname: Takenouchi
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Ogihara
authors.forename: Megumi
authors.initials: M
authors.lastname: Hoshino
authors.forename: Masuhiro
authors.initials: M
authors.lastname: Takata
authors.forename: Morikazu
authors.initials: M
authors.lastname: Imamura
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Tagawa
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hayashi-Kato
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ushiki-Kaku
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okada
authors.forename: Morikazu
authors.initials: M
authors.lastname: Shinagawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitani
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D051379:Mice; D017628:Microglia / Q000166:cytology* / Q000378:metabolism; D018620:PrPSc Proteins / Q000235:genetics / Q000378:metabolism*; D011328:Prions; D012608:Scrapie / Q000473:pathology*
chemical_list: D018620:PrPSc Proteins; D011328:Prions