The parthenogenetic activation of canine oocytes with Ca-EDTA by various culture periods and concentrations.
In the present study, canine oocytes were exposed to various concentrations of and durations of exposure to EDTA saturated with Ca(2+) (Ca-EDTA), a cell membrane-impermeable metal ion chelator, to determine if parthenogenetic activation could be induced. When oocytes were cultured for 48 or 72 h in parthenogenetic activation medium (PAM) without Ca-EDTA (control) or PAM supplemented with 1 or 5mM Ca-EDTA, the highest rate of pronuclear formation (PN) was obtained in oocytes cultured in 1mM Ca-EDTA for 48 h (8.0%; P<0.05). There was no pronuclear formation in the control group (PAM without Ca-EDTA). Oocytes treated with 5mM Ca-EDTA for 48 h or 1mM Ca-EDTA for 72 h formed a parthenogenetic pronucleus (3.1 and 4.5, respectively). However, there was no pronuclear formation in oocytes treated with 5mM Ca-EDTA for 72 h. In summary, exposure to Ca-EDTA can induce pronuclear formation in canine oocytes.
Lee
Sang Ryeul
SR
School of Life Science and Biotechnology, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, Daegu 702-701, Republic of Korea.
Kim
Jae-Wook
JW
Kim
Bong Soo
BS
Kim
Myoung Ok
MO
Kim
Sung Hyun
SH
Yoo
Dong Hun
DH
Shin
Mi-Jung
MJ
Lee
Sanggyu
S
Park
Young Sik
YS
Park
Yong Bok
YB
Ha
Ji Hong
JH
Ryoo
Zae Young
ZY
D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology*; D046508:Culture Techniques / Q000662:veterinary*; D004285:Dogs / Q000502:physiology*; D004492:Edetic Acid / Q000494:pharmacology*; D005260:Female; D009865:Oocytes / Q000187:drug effects*; D010312:Parthenogenesis / Q000187:drug effects*; D013997:Time Factors
D002614:Chelating Agents; D004492:Edetic Acid