[Resistance to HIV-1 infection of CD4 + T lymphocytes in vitro from chinese HIV-1 exposed seronegative individuals].
OBJECTIVE
To determine the relative resistance to HIV-1 infection of CD4 + T lymphocytes in HIV-exposed seronegative individuals (ESNs) in China.


METHODS
HIV primary isolates were obtained from peripheral whole blood of HIV-infected persons. CD4 + T lymphocytes of Chinese ESNs were separated from peripheral blood mononuclear cells with magnetic cell sorting (MACS). The purified CD4 + T lymphocytes were cocultured with HIV primary isolates. The p24 level was detected and the culture medium was refreshed every 3 days within 2 weeks.


RESULTS
For M tropic HIV strains, p24 level was significantly lower in ESN group than in control group (P < 0.05); for some M tropic HIV strains, even no p24 replicated in ESN group. However, T tropic virus strains had no significant difference between these two groups (P > 0.05).


CONCLUSIONS
CD4 + T lymphocytes of Chinese ESNs may possess relative resistance to M tropic HIV strains, which may be one of the main influencing factors that result in ESN.
Su
Yan-li
YL
Center for AIDS Research, the First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Shang
Hong
H
Liu
Jing
J
Wang
Shu-xiang
SX
Geng
Wen-qing
WQ
Cui
Hua-lu
HL
Jiang
Yong-jun
YJ
Wang
Ya-nan
YN
Zhang
Zi-ning
ZN
Wang
Ya-ting
YT
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D002681:China; D005260:Female; D006678:HIV / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity; D015658:HIV Infections / Q000821:virology*; D018023:HIV Seronegativity / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D012747:Sexual Partners