Silybin reduces lipid peroxidation of rat hepatocyte membrane caused by cyclosporin A.
An effect of cyclosporin A on lipid peroxidation in isolated rat hepatocytes was tested. A significant increase in lipid peroxidation marker (the concentration of lipofuscin-like pigments) was observed in samples incubated with cyclosporin A in comparison with the control. When hepatoprotective flavonoid silybin was added, the production of lipofuscin-like pigments decreased significantly. This result indicates a potential positive role of silybin in lowering of cyclosporin A side effects associated with the production of reactive oxygen species and plasma membrane damage.
Moulisová
V
V
Department of Plant Physiology, Faculty of Biological Sciences, University of South Bohemia, Ceske Budejovice, 370 05, Czech Republic. vladimira.moulisova@email.cz
Srbová
M
M
Jedlicková
O
O
Sebestian
J
J
Jegorov
A
A
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D015394:Molecular Structure; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D000077385:Silybin; D012838:Silymarin / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors
D000975:Antioxidants; D008563:Membrane Lipids; D012838:Silymarin; D000077385:Silybin; D016572:Cyclosporine