Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I.
The interactions between oligonucleotides and inorganic cations have been measured by capillary zone electrophoresis. With increasing concentrations of divalent cations (Ca(2+), Mg(2+), Mn(2+) and Ni(2+)) in the running buffer, the migration behavior was evaluated by calculation of the binding constants. Besides these fundamental studies of binding equilibria, different buffer components, tris(hydroxymethyl)aminomethane and 3-(N-morpholino)propanesulfonic acid, have been investigated and their effects on metal ion binding quantified.
Stettler
Alexandra R
AR
Department of Chemistry, University of Basel, Spitalstrasse 51, 4056, Basel, Switzerland.
Chaurin
Valérie
V
Constable
Edwin C
EC
Housecroft
Catherine E
CE
Schwarz
Maria A
MA
D002118:Calcium / Q000737:chemistry; D002413:Cations, Divalent / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008274:Magnesium / Q000737:chemistry; D008345:Manganese / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D009025:Morpholines / Q000737:chemistry; D009532:Nickel / Q000737:chemistry; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D014325:Tromethamine / Q000737:chemistry
D002413:Cations, Divalent; D008670:Metals; D009025:Morpholines; D009696:Nucleic Acids; D009841:Oligonucleotides; D014325:Tromethamine; C008550:morpholinopropane sulfonic acid; D008345:Manganese; D009532:Nickel; D004247:DNA; D008274:Magnesium; D002118:Calcium