Transporter assays using solid supported membranes: a novel screening platform for drug discovery.
Transporters are important targets in drug discovery. However, high throughput-capable assays for this class of membrane proteins are still missing. Here we present a novel drug discovery platform technology based on solid supported membranes. The functional principles of the technology are described, and a sample selection of transporter assays is discussed: the H(+)-dependent peptide transporter PepT1, the gastric proton pump, and the Na(+)/Ca(2+) exchanger. This technology promises to have an important impact on the drug discovery process.
Kelety
Bela
B
IonGate Biosciences, Frankfurt, Germany.
Diekert
Kerstin
K
Tobien
Joanna
J
Watzke
Natalie
N
Dörner
Wolfgang
W
Obrdlik
Petr
P
Fendler
Klaus
K
D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D015195:Drug Design; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D026901:Membrane Transport Proteins / Q000737:chemistry*; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods
D026901:Membrane Transport Proteins; D008567:Membranes, Artificial