Isolation and characterization of a new bacterium capable of biotransforming cis -epoxysuccinic acid to D(-) -tartaric acid.
A bacterial strain 1-3 capable of enantioselectively hydrolyzing cis-epoxysuccinic acid to D(-)-tartaric acid was isolated from soil. Phenotypic characteristics, Biolog GN test and phylogenetic analysis of strain 1-3 indicated that it belongs to the genus Bordetella. cis-Epoxysuccinic acid could be used as a carbon source and inducer for production of cis-epoxysuccinic acid hydrolase. After the purification of the enzymatic biotransformed product, a colorless and scentless crystal was obtained, which was confirmed to be the tartaric acid with laevorotatory optical rotation by the (1)H nuclear magnetic resonance spectrum and optical rotation analysis.
Li
Xia
X
College of Life Sciences, Zhejiang University, Hangzhou, China.
Ma
Xiaohang
X
Zhao
Yuhua
Y
Jia
Xiaoming
X
Kai
Lei
L
Guo
Kangping
K
Zhao
Weifeng
W
D001426:Bacterial Proteins / Q000378:metabolism; D001711:Biotransformation; D001884:Bordetella / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D013386:Succinates / Q000737:chemistry / Q000378:metabolism*; D013644:Tartrates / Q000737:chemistry / Q000378:metabolism*
D001426:Bacterial Proteins; D013386:Succinates; D013644:Tartrates; C017290:2,3-epoxysuccinic acid; D002244:Carbon; D006867:Hydrolases; C029768:tartaric acid