Genome-wide atlas of gene expression in the adult mouse brain.
Molecular approaches to understanding the functional circuitry of the nervous system promise new insights into the relationship between genes, brain and behaviour. The cellular diversity of the brain necessitates a cellular resolution approach towards understanding the functional genomics of the nervous system. We describe here an anatomically comprehensive digital atlas containing the expression patterns of approximately 20,000 genes in the adult mouse brain. Data were generated using automated high-throughput procedures for in situ hybridization and data acquisition, and are publicly accessible online. Newly developed image-based informatics tools allow global genome-scale structural analysis and cross-correlation, as well as identification of regionally enriched genes. Unbiased fine-resolution analysis has identified highly specific cellular markers as well as extensive evidence of cellular heterogeneity not evident in classical neuroanatomical atlases. This highly standardized atlas provides an open, primary data resource for a wide variety of further studies concerning brain organization and function.
Lein
Ed S
ES
Allen Institute for Brain Science, Seattle, Washington 98103, USA. edl@alleninstitute.org
Hawrylycz
Michael J
MJ
Ao
Nancy
N
Ayres
Mikael
M
Bensinger
Amy
A
Bernard
Amy
A
Boe
Andrew F
AF
Boguski
Mark S
MS
Brockway
Kevin S
KS
Byrnes
Emi J
EJ
Chen
Lin
L
Chen
Li
L
Chen
Tsuey-Ming
TM
Chin
Mei Chi
MC
Chong
Jimmy
J
Crook
Brian E
BE
Czaplinska
Aneta
A
Dang
Chinh N
CN
Datta
Suvro
S
Dee
Nick R
NR
Desaki
Aimee L
AL
Desta
Tsega
T
Diep
Ellen
E
Dolbeare
Tim A
TA
Donelan
Matthew J
MJ
Dong
Hong-Wei
HW
Dougherty
Jennifer G
JG
Duncan
Ben J
BJ
Ebbert
Amanda J
AJ
Eichele
Gregor
G
Estin
Lili K
LK
Faber
Casey
C
Facer
Benjamin A
BA
Fields
Rick
R
Fischer
Shanna R
SR
Fliss
Tim P
TP
Frensley
Cliff
C
Gates
Sabrina N
SN
Glattfelder
Katie J
KJ
Halverson
Kevin R
KR
Hart
Matthew R
MR
Hohmann
John G
JG
Howell
Maureen P
MP
Jeung
Darren P
DP
Johnson
Rebecca A
RA
Karr
Patrick T
PT
Kawal
Reena
R
Kidney
Jolene M
JM
Knapik
Rachel H
RH
Kuan
Chihchau L
CL
Lake
James H
JH
Laramee
Annabel R
AR
Larsen
Kirk D
KD
Lau
Christopher
C
Lemon
Tracy A
TA
Liang
Agnes J
AJ
Liu
Ying
Y
Luong
Lon T
LT
Michaels
Jesse
J
Morgan
Judith J
JJ
Morgan
Rebecca J
RJ
Mortrud
Marty T
MT
Mosqueda
Nerick F
NF
Ng
Lydia L
LL
Ng
Randy
R
Orta
Geralyn J
GJ
Overly
Caroline C
CC
Pak
Tu H
TH
Parry
Sheana E
SE
Pathak
Sayan D
SD
Pearson
Owen C
OC
Puchalski
Ralph B
RB
Riley
Zackery L
ZL
Rockett
Hannah R
HR
Rowland
Stephen A
SA
Royall
Joshua J
JJ
Ruiz
Marcos J
MJ
Sarno
Nadia R
NR
Schaffnit
Katherine
K
Shapovalova
Nadiya V
NV
Sivisay
Taz
T
Slaughterbeck
Clifford R
CR
Smith
Simon C
SC
Smith
Kimberly A
KA
Smith
Bryan I
BI
Sodt
Andy J
AJ
Stewart
Nick N
NN
Stumpf
Kenda-Ruth
KR
Sunkin
Susan M
SM
Sutram
Madhavi
M
Tam
Angelene
A
Teemer
Carey D
CD
Thaller
Christina
C
Thompson
Carol L
CL
Varnam
Lee R
LR
Visel
Axel
A
Whitlock
Ray M
RM
Wohnoutka
Paul E
PE
Wolkey
Crissa K
CK
Wong
Victoria Y
VY
Wood
Matthew
M
Yaylaoglu
Murat B
MB
Young
Rob C
RC
Youngstrom
Brian L
BL
Yuan
Xu Feng
XF
Zhang
Bin
B
Zwingman
Theresa A
TA
Jones
Allan R
AR
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism*; D019295:Computational Biology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016678:Genome / Q000235:genetics*; D023281:Genomics; D006624:Hippocampus / Q000033:anatomy & histology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger