Calcium-regulated fusion of yolk granules is important for yolk degradation during early embryogenesis of Rhodnius prolixus Stahl.
This study examined the process of membrane fusion of yolk granules (YGs) during early embryogenesis of Rhodnius prolixus. We show that eggs collected at days 0 and 3 after oviposition contain different populations of YGs, for example day-3 eggs are enriched in large YGs (LYGs). Day-3 eggs also contain the highest free [Ca(2+)] during early embryogenesis of this insect. In vitro incubations of day-0 YGs with [Ca(2+)] similar to those found in day-3 eggs resulted in the formation of LYGs, as observed in vivo. Fractionation of LYGs and small YGs (SYGs) and their subsequent incubation with the fluorescent membrane marker PKH67 showed a calcium-dependent transference of fluorescence from SYGs to LYGs, possibly as the result of membrane fusion. Acid phosphatase and H(+)-PPase activities were remarkably increased in day-3 LYGs and in calcium-treated day-0 LYGs. Both fractions were found to contain vitellins as major components, and incubation of YGs with calcium induced yolk proteolysis in vitro. Altogether, our results suggest that calcium-induced membrane fusion events take part in yolk degradation, leading to the assembly of the yolk mobilization machinery.
Ramos
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IB
Laboratório de Entomologia Médica, Instituto de Biofísica Carlos Chagas Filho (IBCCF), Universidade Federal do Rio de Janeiro (UFRJ), Cidade Universitária--Ilha do Fundão, 21941-590 Rio de Janeiro, RJ, Brasil.
Miranda
K
K
de Souza
W
W
Oliveira
D M P
DM
Lima
A P C A
AP
Sorgine
M H F
MH
Machado
E A
EA
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004527:Egg Proteins / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development; D006867:Hydrolases / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D010063:Ovum / Q000166:cytology*; D017494:Proton Pumps / Q000378:metabolism; D012239:Rhodnius / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D047048:Vitellins / Q000378:metabolism
D004527:Egg Proteins; D017494:Proton Pumps; D047048:Vitellins; D006867:Hydrolases; D002118:Calcium