Overexpression of Dicer enhances RNAi-mediated gene silencing by short-hairpin RNAs (shRNAs) in human cells.
RNA interference (RNAi) is a process in which small-interfering RNA (siRNA) induces a sequence-dependent degradation of mRNA. In recent years, vector-based siRNA expression systems have been developed by several groups. In these systems, short-hairpin RNAs (shRNAs) have been widely chosen for first transcriptional products which are processed to siRNAs by Dicer-dependent cleavage. In this study, we demonstrate that overexpression of Dicer enhances the effect of shRNA-dependent gene silencing in mammalian cells. Therefore, these results indicate that Dicer-dependent cleavage of shRNAs is an important step for shRNA-mediated gene silencing.
Mikuma
Toshiyasu
T
Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba Science City 305-8562, Japan.
Kawasaki
Hiroaki
H
Yamamoto
Yasuhiko
Y
Taira
Kazunari
K
D000818:Animals; D004331:Drosophila melanogaster / Q000201:enzymology*; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008954:Models, Biological; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D043244:Ribonuclease III / Q000235:genetics* / Q000378:metabolism*
D034741:RNA, Small Interfering; D012343:RNA, Transfer; D008156:Luciferases; D043244:Ribonuclease III