title: Proximity ligation: a specific and versatile tool for the proteomic era.
abstract: Knowledge about the total human genome sequence now provides opportunities to study its myriad gene products. However, the presence of alternative splicing, post-translational modifications, and innumerable protein-protein interactions among proteins occurring at widely different concentrations, all combine to place extreme demands on the specificity and sensitivity of assays. The choice of method also depends on matters such as whether proteins will be analyzed in body fluids and lysates, or localized inside single cells. In this review we discuss commonly used detection methods and compare these to the recently-developed proximity ligation technique.
authors.affiliation: University of Uppsala, Department of Genetics and Pathology, Rudbeck laboratory, SE-751 85 Uppsala, Sweden.
authors.forename: Ola
authors.initials: O
authors.lastname: Söderberg
authors.forename: Karl-Johan
authors.initials: KJ
authors.lastname: Leuchowius
authors.forename: Masood
authors.initials: M
authors.lastname: Kamali-Moghaddam
authors.forename: Malin
authors.initials: M
authors.lastname: Jarvius
authors.forename: Sigrun
authors.initials: S
authors.lastname: Gustafsdottir
authors.forename: Edith
authors.initials: E
authors.lastname: Schallmeiner
authors.forename: Mats
authors.initials: M
authors.lastname: Gullberg
authors.forename: Jonas
authors.initials: J
authors.lastname: Jarvius
authors.forename: Ulf
authors.initials: U
authors.lastname: Landegren
mesh_terms: D000818:Animals; D005818:Genetic Engineering; D006801:Humans; D040901:Proteomics / Q000379:methods*