title: Hydrogenosomal activity of Trichomonas vaginalis cultivated under different iron conditions.
abstract: To evaluate whether iron concentration in TYM medium influence on hydrogenosomal enzyme gene expression and hydrogenosomal membrane potential of Trichomonas vaginalis, trophozoites were cultivated in irondepleted, normal and iron-supplemented TYM media. The mRNA of hydrogenosomal enzymes, such as pyruvate ferredoxin oxidoreductase (PFOR), hydrogenase, ferredoxin and malic enzyme, was increased with iron concentrations in T. vaginalis culture media, measured by RT-PCR. Hydrogenosomal membrane potentials measured with DiOC6 also showed similar tendency, e.g. T. vaginalis cultivated in iron-depleted and iron-supplemented media for 3 days showed a significantly reduced and enhanced hydrogenosomal membrane potential compared with that of normal TYM media, respectively. Therefore, it is suggested that iron may regulate hydrogenosomal activity through hydrogenosomal enzyme expression and hydrogenosomal membrane potential.
authors.affiliation: Department of Biochemistry and Molecular Biology, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Yong-Seok
authors.initials: YS
authors.lastname: Kim
authors.forename: Hyun-Ouk
authors.initials: HO
authors.lastname: Song
authors.forename: Ik-Hwa
authors.initials: IH
authors.lastname: Choi
authors.forename: Soon-Jung
authors.initials: SJ
authors.lastname: Park
authors.forename: Jae-Sook
authors.initials: JS
authors.lastname: Ryu
mesh_terms: D000818:Animals; D003470:Culture Media; D005288:Ferredoxins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000235:genetics / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D008564:Membrane Potentials; D015388:Organelles / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology*; D050743:Pyruvate Synthase / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014246:Trichomonas vaginalis / Q000254:growth & development*
chemical_list: D003470:Culture Media; D005288:Ferredoxins; D006859:Hydrogen; D007501:Iron; D008291:Malate Dehydrogenase; D006864:Hydrogenase; D050743:Pyruvate Synthase