Enhanced effect of fluorescent whitening agent on peroral infection for recombinant baculovirus in the host Bombyx mori L.
The low efficiency of the oral infectivity of recombinant polyhedrin-negative baculovirus is a major bottleneck in the application of the baculovirus expression system in the silkworm (Bombyx mori L). In this study, the effects of a fluorescent whitening agent on improving the oral infection for the recombinant Bombyx mori nuclear polyhedrosis virus in silkworm larva and their possible mechanism were investigated. The results showed that the peroral infection can be remarkably enhanced by adding VBL into the larval artificial diet. The maximum infection rate reached as high as 90% with the concentration of VBL (1%), which was then considered as optimal. The total protease activity and pH value of the larval intestinal juice were found to be lower when compared to the control, indicating an abnormal physiological change of the larval digestive system by VBL, which, in turn, resulted in improved peroral infection of recombinant virus.
Wang
Bing
B
Department of Sericulture, Shandong Agricultural University, Taian, China 271018.
Shang
Jinyan
J
Liu
Xunli
X
Cui
Weizheng
W
Wu
Xiaofeng
X
Zhao
Na
N
D010832:6-Phytase / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology*; D012831:Bombyx / Q000276:immunology / Q000821:virology*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000276:immunology; D007814:Larva / Q000276:immunology / Q000821:virology; D009055:Mouth / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000254:growth & development*; D011994:Recombinant Proteins / Q000096:biosynthesis
D001555:Benzene Derivatives; D005456:Fluorescent Dyes; D011994:Recombinant Proteins; C517570:fluorescent whitening agent VBL; D010832:6-Phytase