Promotion of acid-mediated cleavage of oligonucleotide P3'->N5' phosphoroamidates by triplex formation: a novel approach to sequence-specific DNA detection.
The 5'-amino-2',4'-BNA, bearing a locked N-type sugar conformation and a P3'-->N5' phosphoramidate linkage, was found to be rapidly cleaved by acid-mediated hydrolysis when they formed a triplex with the target dsDNA. The fluorophore and quencher moieties were introduced into the 5'-amino-2',4'-BNA-modified oligonucleotide which was used as a novel and innovative DNA detection probe.
Tomizu
Masaharu
M
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Nakagawa
Osamu
O
Obika
Satoshi
S
Imanishi
Takeshi
T
D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D015345:Oligonucleotide Probes / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D013050:Spectrometry, Fluorescence
D015342:DNA Probes; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D009943:Organophosphorus Compounds; C434331:phosphoramidite; C051802:triplex DNA; D004247:DNA