title: DNA fragments induction in human fibroblasts by radiations of different qualities.
abstract: Experimental data on DNA double strand break (DSB) induction in human fibroblasts (AG1522), following irradiation with several radiation qualities, namely gamma rays, 0.84 MeV protons, 58.9 MeV u(-1) carbon ions, iron ions of 115 MeV u(-1), 414 MeV u(-1), 1 GeV u(-1), and 5 GeV u(-1), are presented. DSB yields were measured by calibrated Pulsed Field Gel Electrophoresis in the DNA fragment size range 0.023-5.7 Mbp. The DSB yields show little LET dependence, in spite of the large variation of the latter among the beams, and are slightly higher than that obtained using gamma rays. The highest yield was found for the 5 GeV u(-1) iron beam, that gave a value 30% higher than the 1 GeV u(-1) iron beam. A phenomenological method is used to parametrise deviation from randomness in fragment size spectra.
authors.affiliation: Health and Technology Department, Istituto Superiore di Sanità, Viale Regina Elena 299, Roma, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Esposito
authors.forename: M
authors.initials: M
authors.lastname: Belli
authors.forename: A
authors.initials: A
authors.lastname: Campa
authors.forename: R
authors.initials: R
authors.lastname: Cherubini
authors.forename: G
authors.initials: G
authors.lastname: Cuttone
authors.forename: V
authors.initials: V
authors.lastname: Dini
authors.forename: Y
authors.initials: Y
authors.lastname: Furusawa
authors.forename: S
authors.initials: S
authors.lastname: Gerardi
authors.forename: G
authors.initials: G
authors.lastname: Simone
authors.forename: E
authors.initials: E
authors.lastname: Sorrentino
authors.forename: M A
authors.initials: MA
authors.lastname: Tabocchini
mesh_terms: D002478:Cells, Cultured; D004247:DNA / Q000235:genetics* / Q000528:radiation effects*; D004249:DNA Damage; D053938:DNA Fragmentation / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000502:physiology* / Q000528:radiation effects*; D006801:Humans; D011829:Radiation Dosage
chemical_list: D004247:DNA