Possible presence and role of the promoter sequence for eukaryotic RNA polymerase III in bacteria.
The bacterial repetitive sequence IS1, is a translocatable DNA segment. The internal region of IS1 acts as a cis-element to stimulate RNA synthesis from the upstream promoter. The product of the bacterial artA gene works with this cis-element to stimulate transcription. Eukaryotic genes for small RNAs and short interspersed repetitive elements (SINEs) have internal promoters, transcribed by RNA polymerase III (RNAP III). RNAP III requires the multisubunit protein factor TFIIIC in transcription initiation. TFIIIC contains the B-block binding subunit which recognizes the internal promoter. Here, I report that the eukaryotic RNAP III promoter-like sequence was found in the cis-element of bacterial IS1. Mutations in the cis-element which affect transcription were present in the RNAP III promoter-like sequence. The RNAP III promoter sequence of Alu, which is a human SINE, was cloned into Escherichia coli, and was shown to stimulate bacterial transcription like the cis-element of IS1. Furthermore, the primary structures of ArtA protein and B-block binding subunits were compared. The amino acid sequence of ArtA appeared to be similar to the N- and C-terminal regions conserved in many B-block binding subunits. Prokaryotes and eukaryotes have been thought to have inherent transcription machineries. The results shown here, however, suggest a new aspect of the evolution of the RNAP III transcription machinery.
Matsutani
Sachiko
S
Division of Microbiology, National Institute of Health Sciences, Tokyo, 158-8501, Japan. sachiko@nihs.go.jp
D020087:Alu Elements; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D021122:Protein Subunits; D012320:RNA Polymerase III / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics; D019474:Transcription Factors, TFIII / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D000098832:Transcription Factor TFIIIC
C524635:ArtA protein, E coli; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D021122:Protein Subunits; D014157:Transcription Factors; D019474:Transcription Factors, TFIII; D000098832:Transcription Factor TFIIIC; D012320:RNA Polymerase III