title: Improved reliability of lymphoma diagnostics via PCR-based clonality testing: report of the BIOMED-2 Concerted Action BHM4-CT98-3936.
abstract: The diagnosis of malignant lymphoma is a recognized difficult area in histopathology. Therefore, detection of clonality in a suspected lymphoproliferation is a valuable diagnostic criterion. We have developed primer sets for the detection of rearrangements in the B- and T-cell receptor genes as reliable tools for clonality assessment in lymphoproliferations suspected for lymphoma. In this issue of Leukemia, the participants of the BIOMED-2 Concerted Action CT98-3936 report on the validation of the newly developed clonality assays in various disease entities. Clonality was detected in 99% of all B-cell malignancies and in 94% of all T-cell malignancies, whereas the great majority of reactive lesions showed polyclonality. The combined BIOMED-2 results are summarized in a guideline, which can now be implemented in routine lymphoma diagnostics. The use of this standardized approach in patients with a suspect lymphoproliferation will result in improved diagnosis of malignant lymphoma.
authors.affiliation: Department of Pathology, UMC St Radboud, Nijmegen, The Netherlands.
authors.forename: J H J M
authors.initials: JH
authors.lastname: van Krieken
authors.forename: A W
authors.initials: AW
authors.lastname: Langerak
authors.forename: E A
authors.initials: EA
authors.lastname: Macintyre
authors.forename: M
authors.initials: M
authors.lastname: Kneba
authors.forename: E
authors.initials: E
authors.lastname: Hodges
authors.forename: R Garcia
authors.initials: RG
authors.lastname: Sanz
authors.forename: G J
authors.initials: GJ
authors.lastname: Morgan
authors.forename: A
authors.initials: A
authors.lastname: Parreira
authors.forename: T J
authors.initials: TJ
authors.lastname: Molina
authors.forename: J
authors.initials: J
authors.lastname: Cabeçadas
authors.forename: P
authors.initials: P
authors.lastname: Gaulard
authors.forename: B
authors.initials: B
authors.lastname: Jasani
authors.forename: J F
authors.initials: JF
authors.lastname: Garcia
authors.forename: M
authors.initials: M
authors.lastname: Ott
authors.forename: M L
authors.initials: ML
authors.lastname: Hannsmann
authors.forename: F
authors.initials: F
authors.lastname: Berger
authors.forename: M
authors.initials: M
authors.lastname: Hummel
authors.forename: F
authors.initials: F
authors.lastname: Davi
authors.forename: M
authors.initials: M
authors.lastname: Brüggemann
authors.forename: F L
authors.initials: FL
authors.lastname: Lavender
authors.forename: E
authors.initials: E
authors.lastname: Schuuring
authors.forename: P A S
authors.initials: PA
authors.lastname: Evans
authors.forename: H
authors.initials: H
authors.lastname: White
authors.forename: G
authors.initials: G
authors.lastname: Salles
authors.forename: P J T A
authors.initials: PJ
authors.lastname: Groenen
authors.forename: P
authors.initials: P
authors.lastname: Gameiro
authors.forename: Ch
authors.initials: Ch
authors.lastname: Pott
authors.forename: J J M van
authors.initials: JJ
authors.lastname: Dongen
mesh_terms: D005188:False Negative Reactions; D015321:Gene Rearrangement; D006801:Humans; D008223:Lymphoma / Q000235:genetics* / Q000473:pathology*; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D015203:Reproducibility of Results
chemical_list: D011948:Receptors, Antigen, T-Cell