RNA interference induced by siRNAs modified with 4'-thioribonucleosides.
Short interfering RNAs (siRNAs) variously modified with 4'-thioribonucleosides against the photinus luciferase gene and the renilla luciferase gene were tested for their induction of the RNA interference (RNAi) activity in cultured NIH/3T3 cells. Results indicated that modifications with 4'-thioribonucleosides on siRNA against the photinus luciferase were as potent as natural siRNA. On the other hand, modifications with 4'-thioribonucleosides on natural siRNA against the renilla luciferase improved their RNAi activity. These results suggest that 4'-thioribonucleosides might be potentially useful in the development of novel and effective chemically modified siRNAs.
Hoshika
Shuichi
S
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Minakawa
Noriaki
N
Matsuda
Akira
A
D000818:Animals; D001483:Base Sequence; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D051379:Mice; D041681:NIH 3T3 Cells; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000494:pharmacology; D012263:Ribonucleosides / Q000737:chemistry; D013872:Thionucleosides / Q000737:chemistry*
D034741:RNA, Small Interfering; D012263:Ribonucleosides; D013872:Thionucleosides; D008156:Luciferases