Isoptericola dokdonensis sp. nov., isolated from soil.
A Gram-positive, non-motile, rod- or coccoid-shaped Isoptericola-like bacterium, strain DS-3(T), was isolated from a soil sample from Dokdo, Korea, and its taxonomic position was investigated by a polyphasic approach. The organism grew optimally at 30 degrees C and pH 7.0-8.0. Strain DS-3(T) had the peptidoglycan type based on l-lys-d-Asp, and galactose, glucose, rhamnose and ribose as the whole-cell sugars. It contained MK-9(H(4)) as the predominant menaquinone and anteiso-C(15 : 0) and iso-C(15 : 0) as the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids. The DNA G+C content was 74.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-3(T) was most closely related to members of the genus Isoptericola. Similarity values between the 16S rRNA gene sequences of strain DS-3(T) and the type strains of Isoptericola species ranged from 98.0 to 98.4 %. DNA-DNA relatedness values (11-23 %) and differential phenotypic properties demonstrated that strain DS-3(T) was distinguishable from recognized Isoptericola species. On the basis of phenotypic properties and phylogenetic and genetic distinctiveness, strain DS-3(T) represents a novel species in the genus Isoptericola, for which the name Isoptericola dokdonensis sp. nov. is proposed. The type strain is DS-3(T) (=KCTC 19128(T)=CIP 108921(T)).
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Laboratory of Microbial Function, PO Box 115, Yusong, Taejon, Korea.
Schumann
Peter
P
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Kang
So-Jung
SJ
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Laboratory of Microbial Function, PO Box 115, Yusong, Taejon, Korea.
Jung
Seo-Youn
SY
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Laboratory of Microbial Function, PO Box 115, Yusong, Taejon, Korea.
Oh
Tae-Kwang
TK
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Laboratory of Microbial Function, PO Box 115, Yusong, Taejon, Korea.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S