title: Benzyl butyl phthalate influences actin distribution and cell proliferation in rat Py1a osteoblasts.
abstract: We previously reported that transient administration of phthalates induced actin cytoskeleton disruption in Py1a osteoblasts. However, the mechanism of this transient effect was not elucidated. In this study we provided evidence that the actin cytoskeletal re-established conditions are dependent on new actin expression and synthesis. To assess the role of phthalates in modulating the distribution of actin, confocal and electron microscopy studies were carried out. Results indicated a modification of actin distribution after phthalate administration. In addition, a relation with the nucleoskeletal component lamin A supports the hypothesis that phthalates may participate in regulatory cell processes involving actin in Py1a osteoblasts. The present study also supports the mitogenic effects of phthalates, which involve microfilament disruption, nuclear actin and lamin A. In particular, the increased levels of cyclin D3, which in mammalian cells plays a critical role in G1 to S transition and is a putative proto-oncogene in benzyl butyl phthalate treated cells, suggested a possible effect of the endocrine disruptor in cancer processes.
authors.affiliation: Department of Comparative Morphology and Biochemistry, University of Camerino, Camerino, MC, Italy.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Agas
authors.forename: Maria G
authors.initials: MG
authors.lastname: Sabbieti
authors.forename: Mariolina
authors.initials: M
authors.lastname: Capacchietti
authors.forename: Stefano
authors.initials: S
authors.lastname: Materazzi
authors.forename: Giovanna
authors.initials: G
authors.lastname: Menghi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Materazzi
authors.forename: Marja M
authors.initials: MM
authors.lastname: Hurley
authors.forename: Luigi
authors.initials: L
authors.lastname: Marchetti
mesh_terms: D000199:Actins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D010795:Phthalic Acids / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D000199:Actins; D010795:Phthalic Acids; D012333:RNA, Messenger; C027561:butylbenzyl phthalate