title: [Evaluation of cefoxitin and cefotaxime screening plates for the detection of methicillin-resistance in Staphylococcus aureus].
abstract: Detection of methicillin-resistant Staphylococcus aureus (MRSA) isolates represents a serious problem to low and media level microbiology labs. In this work cefoxitin (FOX) and cefotaxime (CTX) screen plates (AS) (8-16 microg/ml) with and without 4% of NaCl were evaluated to detect MRSA. Sensitivity, specificity, positive and negative predictive values were determined. The AS oxacillin and the agglutination test MRSA-Screen Latex for the detection of PLP2a were used as reference methods for the evaluation of the different studied screening plates. The 100% (94 strains) PLP2a positive were detected as MRSA with FOX (8 microg/ml), and CTX (8 microg/ml with 4% NaCl) AS. The advantage of FOX AS (8 microg/ml) is that it does not need the addition of NaCl, and CTX AS (8 microg/ml with 4% NaCl) is that cefotaxime is an antimicrobial easily accessible in our country.
authors.affiliation: Sección Microbiología, Laboratorio Central, Hospital Dr. José María Cullen Santa Fe, Argentina. roslorenz@hotmail.com
authors.forename: R
authors.initials: R
authors.lastname: Lorenz
authors.forename: E de los A
authors.initials: Ede L
authors.lastname: Méndez
authors.forename: C
authors.initials: C
authors.lastname: Ahumada
authors.forename: A
authors.initials: A
authors.lastname: Nagel
authors.forename: C
authors.initials: C
authors.lastname: Ramos
authors.forename: M A
authors.initials: MA
authors.lastname: Mendosa
authors.forename: M E
authors.initials: ME
authors.lastname: Nardín
authors.forename: S
authors.initials: S
authors.lastname: Morano
authors.forename: A
authors.initials: A
authors.lastname: Mollerach
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002439:Cefotaxime / Q000494:pharmacology*; D002440:Cefoxitin / Q000494:pharmacology*; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D002440:Cefoxitin; D002439:Cefotaxime