Electron probe microanalysis of cytoplasmic concentrations of elements in a single cell in culture and suspension.
Electron probe microanalysis is a method for evaluation of concentrations of elements at the subcellular level, effective in studies of an individual cell in culture or suspension. Intracellular concentrations of cytoplasmic ions (K+, Na+, Cl-) most rapidly and markedly reacting to changes is an adequate criterion for evaluating the traumatism of manipulations used in cell technologies. Using electron probe microanalysis it will be possible to develop special procedures (for example, therapeutic cloning) for reprogramming or cryopreservation, when intactness of intracellular balance of element serves as the criterion of cell status.
Pogorelov
A G
AG
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino.
Gol'dstein
D V
DV
D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D002713:Chlorine / Q000737:chemistry; D003593:Cytoplasm / Q000378:metabolism; D004577:Electron Probe Microanalysis / Q000379:methods*; D004853:Epoxy Resins / Q000737:chemistry; D006801:Humans; D011188:Potassium / Q000737:chemistry; D012964:Sodium / Q000737:chemistry
D004853:Epoxy Resins; D002713:Chlorine; C004875:EPON; D012964:Sodium; D011188:Potassium