title: High throughput purification of amino-modified oligonucleotides and its application to novel detection system of gene expression.
abstract: We report here a new amino-modifier reagent, which enable high-throughput purification of amino-modified oligonucleotides. Either monomethoxytrityl (MMT) or trifluoroacetyl (TFA) group has been used as the protecting group for the primary amine when amino-terminal oligonucleotides are prepared. Generally, the removal of MMT requires the stringent acidic treatment after the oligonucleotide synthesis. We chemically synthesized a novel amino-modifier with the MMT protection. It was found that the new amino-modifier released MMT group under mild acidic condition, and then rapid purification of diverse amino-modified oligonucleotides could be achieved with cartridge column of reverse phase. Furthermore, we tried to construct the new detection system to study gene expression using the amino-modified oligonucleotides. The new amino-modifier will be useful for molecular biology by facilitating the construction of oligonucleotide library and improving the chemical reactivity.
authors.affiliation: Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science & Technology (AIST Hokkaido), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
authors.forename: Naoshi
authors.initials: N
authors.lastname: Kojima
authors.forename: Maiko
authors.initials: M
authors.lastname: Sugino
authors.forename: Akiko
authors.initials: A
authors.lastname: Mikami
authors.forename: Ken
authors.initials: K
authors.lastname: Nonaka
authors.forename: Yumi
authors.initials: Y
authors.lastname: Fujinawa
authors.forename: Yoji
authors.initials: Y
authors.lastname: Ueda
authors.forename: Kousuke
authors.initials: K
authors.lastname: Sato
authors.forename: Eiko
authors.initials: E
authors.lastname: Ohtsuka
authors.forename: Kenichi
authors.initials: K
authors.lastname: Matsubara
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Komatsu
mesh_terms: D000588:Amines / Q000737:chemistry; D020869:Gene Expression Profiling; D007202:Indicators and Reagents; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000588:Amines; D007202:Indicators and Reagents; D015345:Oligonucleotide Probes