title: Rapid pulsed-field gel electrophoresis protocol for subtyping of Streptococcus suis serotype 2.
abstract: A rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping of Streptococcus suis serotype 2 was developed and evaluated using 27 clinical isolates from 22 epidemiologically unrelated patients. Results were matched against antibiogram, virulence genotyping and multi locus sequence typing (MLST). PFGE appeared to be the most discriminatory with numerical index of discrimination (D) equal to 0.87.
authors.affiliation: Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong SAR, China.
authors.forename: Cindy K Y
authors.initials: CK
authors.lastname: Luey
authors.forename: Yiu Wai
authors.initials: YW
authors.lastname: Chu
authors.forename: Terence K M
authors.initials: TK
authors.lastname: Cheung
authors.forename: Catherine C P
authors.initials: CC
authors.lastname: Law
authors.forename: Man Yu
authors.initials: MY
authors.lastname: Chu
authors.forename: Danny T L
authors.initials: DT
authors.lastname: Cheung
authors.forename: Kai Man
authors.initials: KM
authors.lastname: Kam
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D008826:Microbial Sensitivity Tests; D012703:Serotyping; D017011:Streptococcus suis / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity; D013997:Time Factors; D014774:Virulence / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial