The formation of g=2.49-species of cytochrome P450 in the rat liver by PCB126 oral administration: identification of heme axial ligands by EPR spectroscopy.
Rat livers and microsomes were subjected to electron paramagnetic resonance (EPR) measurements at 77 K. The EPR spectra of the livers from the control group, carbon tetrachloride-, 3-methylcholanthrene-, and 3,3',4,4',5-pentachlorobiphenyl (PCB126)-treated rats exhibited an EPR spectrum at g=2.40, 2.24, and 1.93, which is characteristic of P450 in a resting state. The liver of the PCB126-treated rats showed an additional distinct EPR spectrum at g=2.49, 2.26, and 1.87 (g=2.49-species). The heme environmental structure of g=2.49-species was identified by crystal field analysis using three EPR g-values of the microsome treated with various chemicals. These results indicated that g=2.49-species is a hemeprotein with cysteine thiolate at the 5th coordination site, and a nitrogenous ligand at the 6th site.
Morita
Hidetoshi
H
School of Veterinary Medicine, Azabu University, Sagamihara. morita@azabu-u.ac.jp
Yoshikawa
Hiroshi
H
Takizawa
Tatsuya
T
Shirai
Mitsuyuki
M
Akahori
Fumiaki
F
Yoshimura
Tetsuhiko
T
D000284:Administration, Oral; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004578:Electron Spin Resonance Spectroscopy; D004790:Enzyme Induction; D005260:Female; D006418:Heme / Q000378:metabolism*; D008024:Ligands; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D008024:Ligands; D006418:Heme; D003577:Cytochrome P-450 Enzyme System