title: Effect of preparation conditions on the nutrient release properties of alginate-whey protein granular microspheres.
abstract: The effect of preparation conditions on nutrient release from alginate (AL)-whey protein isolate (WPI) granular microspheres obtained by an emulsification/internal cold gelation method was studied by varying WPI/AL ratio, microsphere diameter, total polymer concentration and riboflavin loading. Microsphere size distribution and nutrient encapsulation efficiency (EE) were examined. Riboflavin release profiles were investigated in simulated gastric and intestinal fluids. Values for EE above 80% were obtained for most microspheres, with the notable exceptions of high AL or pure AL. Variations in WPI/AL ratio, granule size and nutrient loading have major impact on nutrient release. Microspheres prepared with a WPI/AL ratio of 8:2, a riboflavin concentration of 1% in the initial aqueous phase and diameters near 94 microm retained the vitamin in SGF and released it in SIF. By careful process design, granular microspheres with potential as oral delivery vehicles for bioactive compounds may be developed.
authors.affiliation: Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Sainte-Foy, Canada.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Chen
authors.forename: Muriel
authors.initials: M
authors.lastname: Subirade
mesh_terms: D000284:Administration, Oral; D000464:Alginates / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004339:Drug Compounding; D004355:Drug Stability; D004655:Emulsions; D005750:Gastric Juice / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007419:Intestinal Secretions / Q000737:chemistry; D008863:Microspheres; D008894:Milk Proteins / Q000737:chemistry*; D010316:Particle Size; D011208:Powders; D012256:Riboflavin / Q000008:administration & dosage / Q000737:chemistry*; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D014803:Vitamin B Complex / Q000008:administration & dosage / Q000737:chemistry*; D000067816:Whey Proteins
chemical_list: D000464:Alginates; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004655:Emulsions; D006603:Hexuronic Acids; D008894:Milk Proteins; D011208:Powders; D000067816:Whey Proteins; D014803:Vitamin B Complex; D020723:Glucuronic Acid; D012256:Riboflavin