[The investigation of FFA fixative solution for retina].
OBJECTIVE
To search for an effective fixative solution for retina.


METHODS
One group of retinas were fixed in an admixture of formalin, dehydrated alcohol and glacial acetic acid (FFA). The other group of retinas were fixed in 10% neutral formalin.HE and immunohistochemistry were performed. Then compare the difference under the light microscope.


RESULTS
The structure of the retinas fixed in FFA fixative solution was clear. The cells were arranged and compacted. Few exfoliation of the tissue from the glass slide was observed.


CONCLUSIONS
FFA fixative solution is superior to 10% neutral formalin for the fixation of retina.
Zhang
Wenxin
W
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. zoc201@yahoo.com
Li
Yongping
Y
Lin
Jianxian
J
Ni
Li
L
Zhang
Huiying
H
D019342:Acetic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D000431:Ethanol / Q000008:administration & dosage* / Q000494:pharmacology; D005404:Fixatives / Q000494:pharmacology*; D005557:Formaldehyde / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000166:cytology*; D016707:Tissue Fixation / Q000379:methods
D005404:Fixatives; D005557:Formaldehyde; D000431:Ethanol; D019342:Acetic Acid