Site-selective RNA scission by PNA-Lu(III) hybrid system.
By using two PNA (PNA = peptide nucleic acid) additives, one-base gap structure was formed in RNA. Upon treatment of this gap-forming RNA substrate with Lu(III) ion, the phosphodiester linkage at 5'-side of the unpaired nucleotide was mainly cleaved. Promising feature of PNA as a sequence recognizing moiety of man-made RNA cutters was evidenced.
Yamamoto
Yoji
Y
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Kimura
Mayumi
M
Kato
Mayu
M
Kuzuya
Akinori
A
Komiyama
Makoto
M
D006868:Hydrolysis; D008187:Lutetium / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry*
D020135:Peptide Nucleic Acids; D008187:Lutetium; D012313:RNA; D012260:Ribonucleases