title: Effect of ret/PTC 1 rearrangement on transcription and post-transcriptional regulation in a papillary thyroid carcinoma model.
abstract: BACKGROUND
  microRNAs (miRNAs) are a group of non-coding single stranded RNAs measuring approximately 22 nt in length that have been found to control cell growth, differentiation and apoptosis. miRNAs negatively regulate their target genes and recently have been implicated in tumourigenesis. Furthermore, miRNA expression profiling correlates with various cancers, with these genes thought to act as both tumour suppressors and oncogenes. ret/PTC 1 is an oncogene with constitutive kinase activity implicated in the development of papillary thyroid carcinoma (PTC). This rearrangement leads to aberrant MAPK activation that is implicated in PTC tumourigenesis.
  
  
  OBJECTIVE
  The aim of this study was to identify the effect that ret/PTC 1 has on transcription and post-transcriptional regulation in PTC by using DNA microarray and microRNA analysis.
  
  
  RESULTS
  DNA microarray analysis revealed a group of genes differentially expressed between normal thyroid cell lines and those harbouring a ret/PTC 1 rearrangement.Furthermore, a unique miRNA expression signature differentiated between PTC cell lines with ret/PTC 1 and a normal thyroid cell line. 21 miRNAs showed significant overexpression and 14 miRNAs showed underexpression in these cell lines when compared to normal thyroid. Several of these up/down regulated miRNAs may be involved in PTC pathogenesis.
authors.affiliation: Dept. of Histopathology, University of Dublin, Trinity College, Dublin, Ireland. sucahill@tcd.ie
authors.forename: Susanne
authors.initials: S
authors.lastname: Cahill
authors.forename: Paul
authors.initials: P
authors.lastname: Smyth
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Finn
authors.forename: Karen
authors.initials: K
authors.lastname: Denning
authors.forename: Richard
authors.initials: R
authors.lastname: Flavin
authors.forename: Esther M
authors.initials: EM
authors.lastname: O'Regan
authors.forename: Jinghuan
authors.initials: J
authors.lastname: Li
authors.forename: Astrid
authors.initials: A
authors.lastname: Potratz
authors.forename: Simone M
authors.initials: SM
authors.lastname: Guenther
authors.forename: Richard
authors.initials: R
authors.lastname: Henfrey
authors.forename: John J
authors.initials: JJ
authors.lastname: O'Leary
authors.forename: Orla
authors.initials: O
authors.lastname: Sheils
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002291:Carcinoma, Papillary / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015321:Gene Rearrangement; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D015514:Oncogene Proteins, Fusion; D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases; C093573:ret-PTC fusion oncoproteins, human