The immunohistochemical outline of p27kip1, cyclin B1 and cyclin D3 in pleomorphic adenomas.
OBJECTIVE
Pleomorphic adenomas of salivary glands are benign lesions which may sometimes relapse even after complete surgical removal. This risk has led to the search for methods to provide predictive data on the biological behaviour of such neoplasia. The authors intend to evaluate the degree of cellular aggression of these tumours by finding prognostic data using the antigens involved in cellular proliferative activity. Therefore they have chosen for this study: p27kip1, cyclin B1 and Cyclin D3.


METHODS
Seventeen mixed tumours, 2 of them relapsed, underwent the direct immunohystochemical PAP technique for the determination of antigens p27kip1, cyclins B1 and D3 of the tissue.


RESULTS
The results obtained show that the verification of these markers may reveal a potential risk of biological deviation and that their expression is independent of the degree of cellularity in neoplasias.


CONCLUSIONS
On the basis of the results, the conclusion is drawn that there is no relation between the expressivity of the mentioned antigens and histological characters of pleomorphic adenomas.
Rullo
R
R
Division of Oral and Maxillofacial Surgery Department of Odontostomatological, Orthodontic and Surgical Sciences, Second University of Naples, Naples, Italy. rosario.rullo@unina2.it
Festa
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VM
Accardo
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Ferraraccio
F
F
D008949:Adenoma, Pleomorphic / Q000737:chemistry* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology; D019926:Cyclin B / Q000032:analysis* / Q000276:immunology; D056744:Cyclin B1; D056743:Cyclin D3; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000032:analysis* / Q000276:immunology; D016213:Cyclins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008875:Middle Aged; D011379:Prognosis; D012468:Salivary Gland Neoplasms / Q000737:chemistry* / Q000473:pathology
D014408:Biomarkers, Tumor; C534699:CCNB1 protein, human; C534696:CCND3 protein, human; D019926:Cyclin B; D056744:Cyclin B1; D056743:Cyclin D3; D016213:Cyclins; D050760:Cyclin-Dependent Kinase Inhibitor p27