Research on biomodulatory effect of natural compounds.
OBJECTIVE
The purpose of this study was to determine whether the extract isolated from the artichoke Cynara cardunculus L. (ECC) had antimutagenic effect and was able to enhance the therapeutic effect of cytostatic drug cis-platinum (cis-Pt).


METHODS
The potential antimutagenic activity of ECC was assayed by a test on sex-linked recessive lethal mutations detection in Drosophila melanogaster males treated with ethylmethane sulfonate (EMS). The possible enhancement of cytostatic/cytotoxic effect of cis-Pt by ECC was evaluated in the cell revitalization assay by measuring cell viability via Trypan blue exclusive assay using mouse leukemia cells L1210.


RESULTS
EMS was both toxic and genotoxic in D. melanogaster males. It statistically significantly increased the frequency of sex-linked recessive lethal mutations in comparison to the negative control. Furthermore, ECC statistically significantly reduced the genotoxic effect of EMS. It acted in a desmutagenic manner via EMS inactivation. In the cell revitalization assay, ECC enhanced the cytotoxic/cytostatic effect of cis-Pt. The therapeutic potential of ECC was established on the basis of statistically significantly lowered recovery of cis-Pt pre-treated mouse leukemia cells in the presence of ECC.


CONCLUSIONS
The results imply that the extract isolated from artichoke C. cardunculus L. has marked beneficial activities antimutagenic and therapeutic effect enhancing) and its potential biomedical application in the combination therapy of cancer and some neurodegenerative diseases may be suggested.
Miadoková
Eva
E
Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia. miadokova@fns.uniba.sk
Nadová
Slavomíra
S
Trebatická
Mária
M
Grolmus
Ján
J
Kopásková
Marcela
M
Rauko
Peter
P
Mucaji
Pavel
P
Grancai
Daniel
D
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D031943:Cynara scolymus / Q000737:chemistry*; D004331:Drosophila melanogaster; D004357:Drug Synergism; D005260:Female; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D016587:Antimutagenic Agents; D000970:Antineoplastic Agents; D010936:Plant Extracts; D002945:Cisplatin