title: Synthesis and application of new amine-modified oligonucleotides using H-phosphonate chemistry.
abstract: Oligodeoxynucleotide probes tethered with amine linkers are commonly used for development of DNA based biosensor tools for studying gene expression and biological assays. However, the current preparation methods for synthesizing amine tethered probes often show defects during acid deprotection and during hydrophobic purification with the amine linker protecting groups. Here, we developed a prospective preparation method of using modified H-phosphonate chemistry to prepare amine tethered oligonucleotide probes. The present developed method will be helpful for preparing efficient amine tethered oligodeoxynucleotide probes that can be used in DNA biosensor applications.
authors.affiliation: Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan.
authors.forename: Nagendra Kumar
authors.initials: NK
authors.lastname: Kamisetty
authors.forename: Seung Pil
authors.initials: SP
authors.lastname: Pack
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nonogawa
authors.forename: Kamakshaiah Charyulu
authors.initials: KC
authors.lastname: Devarayapalli
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kodaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Makino
mesh_terms: D000588:Amines / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000737:chemistry; D063065:Organophosphonates / Q000737:chemistry
chemical_list: D000588:Amines; D015345:Oligonucleotide Probes; D063065:Organophosphonates