title: Selective enrichment of Ser-/Thr-phosphorylated peptides in the presence of Ser-/Thr-glycosylated peptides.
abstract: Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these conditions to achieve a beta-elimination that is highly selective for phosphorylated peptides. This is the first report of selective beta-elimination and enrichment of phosphorylated peptides in the presence of glycosylated peptides.
authors.affiliation: Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
authors.forename: Alex J
authors.initials: AJ
authors.lastname: Poot
authors.forename: Eelco
authors.initials: E
authors.lastname: Ruijter
authors.forename: Timo
authors.initials: T
authors.lastname: Nuijens
authors.forename: Eef H C
authors.initials: EH
authors.lastname: Dirksen
authors.forename: Albert J R
authors.initials: AJ
authors.lastname: Heck
authors.forename: Monique
authors.initials: M
authors.lastname: Slijper
authors.forename: Dirk T S
authors.initials: DT
authors.lastname: Rijkers
authors.forename: Rob M J
authors.initials: RM
authors.lastname: Liskamp
mesh_terms: D006020:Glycopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D010748:Phosphopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics; D012694:Serine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013912:Threonine / Q000737:chemistry
chemical_list: D006020:Glycopeptides; D009842:Oligopeptides; D010748:Phosphopeptides; D013912:Threonine; D012694:Serine