[Regeneration of transgenic Lespedeza thunbergii expressing levansucrase gene (Sac B) from Bacillus subtilis].
The Bacillus subtilis Sac B gene with the vacuolar targeting signal sequence driven by 35S promotor was transferred into Lespedeza thunbergii by Agrobacterium mediated method. Total 62 Kan-resistant plants were obtained, of which 5 plants were proved to be transgenic plants. The transgenic plants were characterized by PCR amplification, PCR-Southern hybridization and RT-PCR. The physiological assay results showed that the transgenic plants were more tolerant to stress than the controls under the condition of 200mmol/L NaCl and 5% PEG, respectively, and that the content of soluble sugar in trnsgenic plants was significantly higher than that of controls in the period of tests (5-15 days) under salt and PEG stress.
Du
Jin-You
JY
Hebei Normal University of Science and Technology, Qinhuangdao 066600, China.
Chen
Xiao-Yang
XY
Zhang
Gui-Rong
GR
Li
Wei
W
Hu
Dong-Nan
DN
Hu
Zan-Min
ZM
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D006602:Hexosyltransferases / Q000235:genetics*; D029884:Lespedeza / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology; D012995:Solubility; D013312:Stress, Physiological / Q000187:drug effects; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics
D002241:Carbohydrates; D012965:Sodium Chloride; D006602:Hexosyltransferases; C021513:levansucrase