Analysis of molecular biology techniques for the diagnosis of human papillomavirus infection and cervical cancer prevention.
The objective of the present study was to evaluate the usefulness of molecular methodologies to access human papillomavirus genome in the genital tract. Samples from 136 women aged 17 to 52 years old obtained from the Dr. Sérgio Franco Laboratories between 2000 and 2001, were analyzed by the hybrid capture assay and amplified by PCR with generic primers MY09/MY11 and specific primers for types 16, 18, 31, 33, 35, 58. Viral genome was detected in 71.3% of the samples by hybrid capture and 75% by amplification. When cytopathology was used as a reference method for screening lesions, hybrid capture (p=0) and amplification (p=0.002) presented positive association. The 3 methods showed absolute agreement when cytopathology confirmed papillomavirus infection and high grade intraepithelial lesion. Disagreements occurred for 10 cases: seven inflammatory cases positive by PCR and negative for hybrid capture and 3 low squamous intraepithelial lesions positive for hybrid capture but negative for amplification. In conclusion, hybrid capture was shown to be sensitive and specific enough for use in clinical routines. Moreover, the evaluation of viral load values obtained by this method were shown to be related to the severity of the lesion and merit further studies to analyze the possible association with risk of progression to malignancy.
Carestiato
Fernanda Nahoum
FN
Laboratório de Diagnóstico Virológico, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, RJ.
Silva
Kátia Cristina da
KC
Balthazar
Daniela Signorelli
DS
Silva
Licínio
L
Marinho
Marisa
M
Oliveira
Ledy Horto Dos Santos
LH
Cavalcanti
Silvia Maria Baeta
SM
D000293:Adolescent; D000328:Adult; D005260:Female; D016679:Genome, Viral; D006801:Humans; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D002577:Uterine Cervical Diseases / Q000175:diagnosis / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000517:prevention & control / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000517:prevention & control / Q000821:virology