Influence of bioreactor scale and complex medium on probing control of glucose feeding in cultivations of recombinant strains of Escherichia coli.
The objective of this work was to evaluate the performance of a feedback glucose control strategy (the probing strategy) in production relevant bioreactors with complex and mineral media. Experimental results from fed-batch cultivations with two recombinant Escherichia coli constructs expressing two different human therapeutic proteins were used to assess the performance and limitations of the glucose probing technique. Even though the performance of the probing strategy was affected by scale and complex media, this methodology rapidly identified a glucose feed protocol similar to an experimentally derived feed regime. This methodology may serve as a powerful tool for industrial process development and in optimization of glucose feed regimes when transferring process technology from one bioreactor system to another.
Velut
Stéphane
S
Automation, Carl Bro AB, Carl Gustafs väg 4, 20509 Malmö, Sweden. stephane.velut@carlbro.se
Castan
Andreas
A
Short
Kevin A
KA
Axelsson
Jan Peter
JP
Hagander
Per
P
Zditosky
Barry A
BA
Rysenga
Christopher W
CW
de Maré
Lena
L
Haglund
Jan
J
D019149:Bioreactors / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007218:Industrial Microbiology; D010100:Oxygen / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
D003470:Culture Media; D011994:Recombinant Proteins; D005947:Glucose; D010100:Oxygen