title: [Cloning and expression of the genes encoding glycerol dehydratase reactivase and identification of its biological activity].
abstract: The gdrA, gdrB gene coding glycerol dehydratase reactivase factor were amplified by using the genomic DNA of Klebsiella pneumoniae as the template. The gdrA and gdrB were inserted in pMD-18T to yield the recombinant cloning vector pMD-gdrAB. After the DNA sequence was determined, the gdrAB gene was subcloned into expression vector pET-28a(+) to yield the recombinant expression vector pET-28gdrAB. Under screening pressure by ampicillin and kanamycin simultaneously, the activity of glycerol dehydratase reactivase was characterized by coexpression of pET-32gldABC, which carry the gldABC gene encoding glycerol dehydratase, and pET-28gdrAB in E. coli BL21(DE3).
authors.affiliation: Key Laboratory of Industrial Biotechnology Fujian Province, Hua Qiao University, Quanzhou 362021, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.forename: Bai-Shan
authors.initials: BS
authors.lastname: Fang
authors.forename: Yan
authors.initials: Y
authors.lastname: Hong
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Wang
authors.forename: Jin-Xia
authors.initials: JX
authors.lastname: Lin
authors.forename: Gui-Lan
authors.initials: GL
authors.lastname: Liu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006836:Hydro-Lyases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; D006836:Hydro-Lyases; C120061:glycerol dehydratase reactivating factor, Klebsiella pneumoniae