Rapid site-selective hydrolysis of double-stranded DNA by use of Ce(IV)/EDTA and PNA bearing phosphate group.
In order to hydrolyze double-stranded DNA efficiently at the target site, two pseudo-complementary peptide nucleic acids (pcPNAs) bearing phosphate group were combined with Ce(IV)/EDTA complex (EDTA = ethylenediamine-N,N,N',N'-tetraacetate). The phosphate groups as metal-binding ligands were placed near the target site, and concentrated the Ce(IV) complex thereto. As the result, the site-selective hydrolysis was notably promoted, compared with the scission by the cutters involving unmodified pcPNAs.
Aiba
Yuichiro
Y
Research center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Mori
Masao
M
Yamamoto
Yoji
Y
Komiyama
Makoto
M
D002563:Cerium; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D003851:Deoxyribonucleases / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry*; D006868:Hydrolysis; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry
D004277:DNA, Single-Stranded; D020135:Peptide Nucleic Acids; D010710:Phosphates; D002563:Cerium; D004247:DNA; D004492:Edetic Acid; D003851:Deoxyribonucleases