Pulmonary effects and biopersistence of deposited silicon carbide whisker after 1-year inhalation in rats.
Silicon carbide whisker (SiCW) is a durable asbestos substitute. To clarify the biopersistence, the changes of geometrical configurations of deposited whiskers, and potential carcinogenicity and fibrogenicity, 42 Wistar male rats were exposed to SiCW for 6 h/day, 5 days/wk for 1 yr by inhalation. The mass median aerodynamic diameter (geometric standard deviation, GSD), the geometric mean fiber diameter (GSD), and the geometric mean fiber length (GSD) were 2.4 microm (2.4), 0.5 microm (1.5) and 2.8 microm (2.3), respectively. The daily average exposure concentrations were 2.6 +/- 0.4 mg/m3 (98 +/- 19 fibers/ml) and the rats were sacrificed at 6 days and 3, 6, and 12 mo after the exposure. The SiCW amount deposited in each rat lung at 6 days after the exposure, determined by an x-ray diffraction method, was 5.3 +/- 1.4 mg. The biological half-time (BHT) was 16 mo calculated from the deposited SiCW at 6 days and 3, 6, and 12 mo. The BHT was more prolonged than normal physiological clearance. The diameter of SiCW in the lung at each sacrificed time during 12 mo of clearance after the inhalation did not change. However, as for the length of SiCW in the lung, longer SiCW tended to be retained in the lung as the clearance time increased, especially after 6 mo. The histopathological examination revealed bronchoalveolar hyperplasia (BAH) in 2 rats at 1 yr after the exposure and severe fibrotic changes around aggregated SiCW.
Akiyama
Izumi
I
Occupational Health Care Office, Mishima Plant, Toray Industries, Inc., Shizuoka, Japan. Izumi_Akiyama@nts.toray.co.jp
Ogami
Akira
A
Oyabu
Takako
T
Yamato
Hiroshi
H
Morimoto
Yasuo
Y
Tanaka
Isamu
I
D000280:Administration, Inhalation; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D017554:Carbon Compounds, Inorganic / Q000493:pharmacokinetics / Q000633:toxicity*; D006965:Hyperplasia / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D008136:Longevity / Q000187:drug effects; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D019016:Mineral Fibers / Q000633:toxicity; D009929:Organ Size / Q000187:drug effects; D010316:Particle Size; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D017655:Silicon Compounds / Q000493:pharmacokinetics / Q000633:toxicity*; D014961:X-Ray Diffraction
D017554:Carbon Compounds, Inorganic; D019016:Mineral Fibers; D017655:Silicon Compounds; C022088:silicon carbide