title: Methodological improvements on extraction of nuclear proteins and its preliminary analysis during the maize (Zea mays L.) endosperm development.
abstract: A procedure to obtain endosperm protein extracts was standardized. After confirming the enrichment with nuclear proteins by immunodetection, the protein profiles of extracts from different seed development stages were compared by SDS-PAGE that showed the existence of several differentially expressed proteins.
authors.affiliation: Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro / Av. Alberto Lamego 2000-CEP 28015-602-Campos dos Goytacazes- RJ-Brasil. ferreirabs@uenf
authors.forename: B S
authors.initials: BS
authors.lastname: Ferreira
authors.forename: A T
authors.initials: AT
authors.lastname: Branco
authors.forename: M A
authors.initials: MA
authors.lastname: de Oliveira
authors.forename: M G
authors.initials: MG
authors.lastname: Pereira
authors.forename: G A
authors.initials: GA
authors.lastname: de Souza Filho
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D009687:Nuclear Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011489:Protein Denaturation; D012639:Seeds / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*
chemical_list: D009687:Nuclear Proteins