title: The development of crystallization and X-ray crystallography method of long stem DNA.
abstract: We clarified stabilization mechanism of left-handed Z-DNA by X-ray crystal structure analysis. I was able to achieve it by burying big minor groove of Z-DNA because metal ion and polyamine connected it to minor groove of Z-DNA. On that occasion I understood that an electric charge and length of polyamine gave big influence to a combination brain very much. I succeeded in crystallization of Z-DNA of a long chain, X-ray crystal structure analysis by examining these combination ability.
authors.affiliation: Osaka University of Pharmaceutical Sciences 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ohishi
authors.forename: Koji
authors.initials: K
authors.lastname: Tukamoto
authors.forename: Mamiko
authors.initials: M
authors.lastname: Odoko
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Grzeskowiak
authors.forename: Naoyoshi
authors.initials: N
authors.lastname: Maezaki
authors.forename: Nobuo
authors.initials: N
authors.lastname: Okabe
authors.forename: Tetsuaki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Ishida
mesh_terms: D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray; D043542:DNA, Z-Form / Q000737:chemistry*; D008670:Metals / Q000737:chemistry; D009690:Nucleic Acid Conformation; D011073:Polyamines / Q000737:chemistry
chemical_list: D043542:DNA, Z-Form; D008670:Metals; D011073:Polyamines