Insect satiety: sulfakinin localization and the effect of drosulfakinin on protein and carbohydrate ingestion in the blow fly, Phormia regina (Diptera: Calliphoridae).
Sulfakinins, which are satiety factors in invertebrates, have previously been shown to inhibit feeding in the German cockroach and desert locust. This study examines the occurrence of sulfakinin immunoreactivity and the role of sulfakinin as a feeding satiety factor in the black blow fly, Phormia regina. Specifically, this study examines the effect of sulfakinin on two of the blow fly's nutrient requirements (i.e., carbohydrates and proteins). We observed sulfakinin immunoreactive cells in the brains of both male and female flies. We found that drosulfakinin I (DrmSKI, FDDY[SO(3)H]GHMRFa) significantly inhibited carbohydrate feeding by 44% at the most effective dose (10 nmol) in female flies. Statistically, there was no significant effect on males; however, injections of 10 nmol DrmSKI reduced carbohydrate feeding by 34% compared to the sham. Drosulfakinin had no effect on protein feeding and no significant inhibition was detected in females or males. The results of this study lend further support to the idea that carbohydrate and protein feeding are regulated by separate control mechanisms, especially in Calliphoridae.
Downer
K E
KE
Department of Plant, Soil and Insect Sciences, Division of Entomology, University of Massachusetts, Amherst, MA 01003, USA. kelley.e.downer@aphis.usda.gov
Haselton
A T
AT
Nachman
R J
RJ
Stoffolano
J G
JG
D000818:Animals; D001921:Brain / Q000378:metabolism; D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D004175:Diptera / Q000187:drug effects* / Q000378:metabolism; D005247:Feeding Behavior / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005724:Ganglia / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D009479:Neuropeptides / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D021381:Protein Transport; D012528:Satiety Response / Q000502:physiology*; D012727:Sex Characteristics
D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D036341:Intercellular Signaling Peptides and Proteins; D009479:Neuropeptides; D009842:Oligopeptides; D010455:Peptides; C056936:drosulfakinin 1; C056938:drosulfakinin II