Comparison of artificial synthesis methods of gene.
The comparative study of the methods for constructing the artificial genes have done. Equine interferon alpha-1 gene and porcine interferon alpha-1 gene were synthesized respectively by several methods from short DNA oligomers and each gene was evaluated about the accuracy of the gene sequence. The gene made by the SPR method was the most accurate.
Tsuchiya
Yoshinori
Y
Exotic Disease Research Station, National Institute of Animal Health, Kodaira, Tokyo, Japan.
Morioka
Kazuki
K
Shirai
Junsuke
J
Yoshida
Kazuo
K
Inumaru
Shigeki
S
D000818:Animals; D004259:DNA-Directed DNA Polymerase; D005813:Genes, Synthetic; D005821:Genetic Techniques; D006736:Horses / Q000235:genetics; D016898:Interferon-alpha / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D013552:Swine / Q000235:genetics
D016898:Interferon-alpha; D009838:Oligodeoxyribonucleotides; D004259:DNA-Directed DNA Polymerase