Rational installation of an allosteric effector on a designed ribozyme.
We designed and constructed an allosterically controllable ribozyme with rational molecular design. An allosteric modular unit that is under the control of an organic molecule was installed to an artificially designed ribozyme on the basis of the fact that the RNA is an assemblage of functional modular units. The ribozyme was successfully converted to allosterically controllable form.
Kuramitsu
Shohei
S
Graduate School of Biostudies, Kyoto University, 606-8502, Kyoto, Japan.
Ikawa
Yoshiya
Y
Inoue
Tan
T
D000494:Allosteric Regulation; D001483:Base Sequence; D005818:Genetic Engineering; D008969:Molecular Sequence Data; D016337:RNA, Catalytic / Q000737:chemistry* / Q000187:drug effects; D013806:Theophylline / Q000494:pharmacology
D016337:RNA, Catalytic; D013806:Theophylline