title: Spectroscopic analysis of DNA duplexes including T-T mismatches.
abstract: The DNA duplex with tandem T x T mismatches has been studied by NMR spectroscopy. For this study, we synthesized decamer duplex with two successive T x T mismatches, TT10:d(CGCGTTGTCC) x d(GGACTTCGCG). From two-dimensional (2D) 1H-1H NOESY spectrum, we were able to trace sequential NOE walks between base protons and anomeric protons (H1'), and assigned all the base protons and anomeric protons. These results indicate that the overall structure of the duplex is a right-handed duplex, with two successive T x T mismatches stacked in the helix.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Shuji
authors.initials: S
authors.lastname: Oda
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kondo
authors.forename: Akira
authors.initials: A
authors.lastname: Ono
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis* / Q000235:genetics; D011740:Pyrimidine Dimers / Q000032:analysis* / Q000235:genetics
chemical_list: D009692:Nucleic Acid Heteroduplexes; D011740:Pyrimidine Dimers; D004247:DNA