title: Suitable restriction enzymes for pulsed-field gel electrophoresis analysis of Candida tropicalis.
abstract: This study was designed to pursue the identification of optimal restriction enzyme for pulsed-field gel electrophoresis (PFGE) analysis of Candida tropicalis. Twelve restriction enzymes were tested on C. tropicalis strains. Our results indicate that BssHII, NaeI, and RsrII were useful enzymes for PFGE analysis, and NaeI was the best for PFGE analysis of C. tropicalis isolates.
authors.affiliation: Mycotic Diseases Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taipei 11561, Taiwan.
authors.forename: Hsiao-Hui
authors.initials: HH
authors.lastname: Chou
authors.forename: Chien-Shun
authors.initials: CS
authors.lastname: Chiou
authors.forename: Kuo-Wei
authors.initials: KW
authors.lastname: Chen
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Li
mesh_terms: D041022:Candida tropicalis / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D004271:DNA, Fungal / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005838:Genotype; D006801:Humans; D016533:Mycological Typing Techniques
chemical_list: D004271:DNA, Fungal; C060630:endodeoxyribonuclease NaeI; C043032:endodeoxyribonuclease RsrII; D015252:Deoxyribonucleases, Type II Site-Specific