Fluorecent staining for study of extracellular polymeric substances in membrane biofouling layers.
Membrane biofouling by microbial products adversely impacts the feasibility of adopting membrane bioreactors (MBRs) for treating wastewater. The fouling layer structure determines the pressure drop across the fouling layer. Three-dimensional distributions of nucleic acids, proteins, alpha-D-GLUCOPYRANOSE POLYSACCHARIDES, AND B-D-glucopyranose polysaccharides in the fouling layer formed on a mixed cellulose ester membrane were generated utilizing a quadruple staining protocol combined with confocal laser scanning microscopy (CLSM). For the first time, this study constructed a three-dimensional volumetric grids model representing the fouling layer structure on the basis of a series of CLSM images. Quantitative structural information about the fouling layer was extracted from the CLSM images.
Chen
Ming-Yuan
MY
Chemical Engineering Department, National Taiwan University, Taipei, Taiwan.
Lee
Duu-Jong
DJ
Yang
Z
Z
Peng
X F
XF
Lai
J Y
JY
D001419:Bacteria / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D001704:Biopolymers / Q000032:analysis; D019149:Bioreactors; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000494:pharmacology; D007834:Lasers; D008567:Membranes, Artificial; D018613:Microscopy, Confocal; D011108:Polymers / Q000032:analysis* / Q000737:chemistry; D012722:Sewage; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical; D018508:Water Purification / Q000295:instrumentation* / Q000379:methods
D001704:Biopolymers; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D011108:Polymers; D012722:Sewage; D014874:Water Pollutants, Chemical