Seeing is believing: real-time cellular activity assay for phospholipase A2.
Quantitative real-time in situ activity assays are necessary for determining the physiological function and regulation of enzymes. A paper in this issue reports the synthesis of a series of new fluorogenic phospholipids that allow fast real-time measurements of cellular activity and head group selectivity of an important family of enzyme, phospholipases.
Cho
Wonhwa
W
Department of Chemistry (M/C 111), 845 West Taylor Street, University of Illinois at Chicago, Chicago, Illinois 60607-7061, USA. wcho@uic.edu
D003199:Computer Systems; D004789:Enzyme Activation / Q000502:physiology; D007424:Intracellular Fluid / Q000201:enzymology*; D010741:Phospholipases A / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D054467:Phospholipases A2
D010741:Phospholipases A; D054467:Phospholipases A2