title: bft gene subtyping in enterotoxigenic Bacteroides fragilis isolated from children with acute diarrhea.
abstract: Enterotoxigenic Bacteroides fragilis (ETBF) strains are associated with diarrhea disease in farm animals and young children. In this study, the bft gene subtyping from ETBF strains recovered from one immunodeficient and two immunocompetent children with diarrhea were determined. Thirteen ETBF strains were isolated and by using a multiplex-PCR their bft subtypes were determined. All 13 ETBF strains harbored the bft-1 subtype and by AP-PCR they were clustered in the same group I. This study shows that ETBF strains can be present in acute diarrhea and that bft-1 subtype is often present in these organisms. However, further studies are needed to evaluate the role of this bft-1 subtype in the pathogenesis of diarrhea.
authors.affiliation: Laboratório de Anaeróbios, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil.
authors.forename: Viviane
authors.initials: V
authors.lastname: Nakano
authors.forename: Tânia A T
authors.initials: TA
authors.lastname: Gomes
authors.forename: Mônica A M
authors.initials: MA
authors.lastname: Vieira
authors.forename: Rita de Cássia
authors.initials: Rde C
authors.lastname: Ferreira
authors.forename: Mario Julio
authors.initials: MJ
authors.lastname: Avila-Campos
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000208:Acute Disease; D001427:Bacterial Toxins / Q000235:genetics*; D001441:Bacteroides fragilis / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D002648:Child; D003967:Diarrhea / Q000382:microbiology*; D006678:HIV; D019073:HT29 Cells; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D001427:Bacterial Toxins; C405031:Bacteroides fragilis toxin; D008666:Metalloendopeptidases