[Comparative study on in vitro stationary culture system or superfusion culture system of cow mammary tissue].
Dairy cow mammary tissue was cultured in superfusion system or stationary system, and the influence of these two methods in the activity and ultrastructure of tissue was investigated according to LDH vigor, trypan blue dying, agrose gel electrophoresis, transmission microscope observation. The results showed that the mammary tissue cultured in superfusion system could keep normal tissue activity and ultrastructure within 12-60 h in DMEM plus 10% calf serum, while mammary tissue stationary culture could keep normal tissue activity and ultrastructure within 60-108 h. Both culture systems had some advantages and disadvantages.
Di
He-Shuang
HS
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Du
Juan
J
Wang
Li-Gang
LG
Yang
Yuan-Yuan
YY
Wang
Gen-Lin
GL
D000818:Animals; D019149:Bioreactors; D002417:Cattle; D005260:Female; D007700:Kinetics; D008321:Mammary Glands, Animal / Q000648:ultrastructure; D008854:Microscopy, Electron; D010477:Perfusion; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000379:methods*