Engagement of the CD160 activating NK cell receptor leads to its association with CD2 in circulating human NK cells.
CD160 activating NK cell receptor is a glycosylphosphatidylinositol-anchored molecule. Upon specific engagement by its HLA class I physiological ligand, CD160 NK cell receptor triggers cytotoxicity and release of different cytokines, including IL-6. How CD160 intracellular signaling is mediated following its specific ligation is unknown. One key element of this signaling may be through the co-activation of other receptors associated to CD160. Using confocal microscopy and freshly isolated human peripheral blood NK cells, we observed a co-polarization of CD160 and CD2 after their respective specific engagement. These results demonstrate that CD160 activating NK cell receptor, upon its specific engagement, physically associates to the CD2 co-activating receptor present in the lipid raft that might play a role as signaling molecule.
Rabot
Magali
M
INSERM U563, CHU Purpan, Toulouse, France.
Bensussan
Armand
A
Le Bouteiller
Philippe
P
D015703:Antigens, CD / Q000378:metabolism*; D018801:CD2 Antigens / Q000378:metabolism*; D058851:GPI-Linked Proteins; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D021962:Membrane Microdomains / Q000276:immunology; D018613:Microscopy, Confocal; D011971:Receptors, Immunologic / Q000378:metabolism*
D015703:Antigens, CD; C114505:CD160 protein, human; D018801:CD2 Antigens; D058851:GPI-Linked Proteins; D015395:Histocompatibility Antigens Class I; D011971:Receptors, Immunologic