title: A novel RNA synthetic method with a 2'-O-(2-cyanoethoxymethyl) protecting group.
abstract: A novel method for the synthesis of RNA oligomers with 2-cyanoethoxymethyl (CEM) as the 2'-hydroxyl protecting group has been developed. The new method allows the synthesis of oligonucleotides with an efficiency and final purity comparable to that obtained in DNA synthesis.(1) In addition, the CEM method has the potential for application to the synthesis of very long RNA oligonucleotides.
authors.affiliation: Discovery Research Laboratories, Nippon Shinyaku Co. Ltd., 3-14-1 Sakura, Tsukuba City, Ibaraki 305-0003, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Shiba
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kitagawa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Masutomi
authors.forename: Kouichi
authors.initials: K
authors.lastname: Ishiyama
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Ohgi
authors.forename: Junichi
authors.initials: J
authors.lastname: Yano
mesh_terms: D005019:Ethyl Ethers / Q000737:chemistry*; D008738:Methyl Ethers / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: C522071:2-cyanoethyl chloromethyl ether; D005019:Ethyl Ethers; D008738:Methyl Ethers; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D012313:RNA