Functional role of the cofactor on activation process of L-histidine dependent ribozyme.
The activity of an L-histidine dependent ribozyme was analyzed with many pseudo-cofactors to investigate the activation process of the ribozyme. As a result, only L-histidine was able to induce the ribozyme activity among all chemicals used in this study. On the other hand, many L-histidine analogues inhibited the activation of the ribozyme by L-histidine, indicating the binding site permitted the analogues' access. These results suggest that the ribozyme recognizes L-histidine and express its activity highly specifically via an activation process followed by the histidine binding.
Kawakami
Junji
J
Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Maiya
Chisako
C
Sugimoto
Naoki
N
D003067:Coenzymes / Q000378:metabolism*; D004789:Enzyme Activation; D006639:Histidine / Q000031:analogs & derivatives / Q000378:metabolism*; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*
D003067:Coenzymes; D016337:RNA, Catalytic; D006639:Histidine