[Effect of high volume hemofiltration on pulmonary surfactant protein in endotoxin induced acute lung injury in dog].
OBJECTIVE
To explore the effect of high volume hemofiltration (HVHF) on pulmonary surfactant protein (SP) in endotoxin induced acute lung injury (ALI) in dogs.


METHODS
Sixteen healthy male mongrel dogs were given lipopolysaccharide(LPS 650 mug/kg) via central vein within 30 minutes. After the reproduction of the model, they were divided into two groups randomly (n=8). One group received the treatment of HVHF and mechanical ventilation (MV, treatment group), while another received only MV (model group). Parameters of arterial blood gas and respiratory mechanics were recorded at basic values, after reproduction of the experimental model, and 1, 2 and 4 hours after HVHF. Content of SP-B in lung tissue homogenate was measured by protein Western blot.


RESULTS
After injection of LPS, partial pressure of oxygen in artery (PaO(2)) and PaO(2)/fractional concentration of inspired oxygen (FiO(2)) began to decrease (both P<0.05). PaO(2)/FiO(2) <300 mm Hg (1 mm Hg=0.133 kPa) when ALI was reproduced. PaO(2) and PaO(2)/FiO(2) were higher in treatment group than those in model group 4 hours after HVHF (both P<0.01). Inspiratory resistance of airway (Raw) and peak inspiratory pressure (PIP) in model group were kept stable after MV. Lung dynamic compliance (Cdyn) and lung total compliance (Ctot) in model group were both decreased while ventilatory work of breathing (WOBvent) increased 4 hours after MV (all P<0.01). All parameters in the treatment group were kept stable and differences in Cdyn and Ctot were significant at 4 hours after HVHF compared to model group (P<0.01 and P<0.05). Content of SP-B in lung tissue homogenate was significantly higher in treatment group than that in model group (P<0.01).


CONCLUSIONS
HVHF could effectively increase the content of SP-B in lung to prevent aggravation of respiratory mechanics and improve oxygenation.
Zeng
Zhen-guo
ZG
Intensive Care Unit,the First Hospital Affiliated to Nanchang University, Nanchang 330006, Jiangxi, China.
Liu
Fen
F
Jiang
Rong
R
Zhu
Feng
F
Nie
Cheng
C
Qian
Ke-jian
KJ
D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism* / Q000628:therapy; D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D006440:Hemofiltration; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000378:metabolism; D008297:Male; D037701:Pulmonary Surfactant-Associated Protein B / Q000378:metabolism*
D008070:Lipopolysaccharides; D037701:Pulmonary Surfactant-Associated Protein B