title: Suppression of HCV RNA replication by baculovirus-mediated shRNA expression.
abstract: We describe a HCV core-short-hairpin RNA (shRNA) expressed from a human U6 Pol II promoter, which synergistically enhanced the inhibition of HCV replication in HCV replicon. We also describe the construction of recombinant baculovirus expressing shRNAs of the HCV core gene under the control of the U6 promoter. The anti-HCV shRNAs may provide a practical basis for applying shRNA-based gene therapy to the treatment of HCV.
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Kaneko
authors.forename: Nobushige
authors.initials: N
authors.lastname: Tamai
authors.forename: Naoko
authors.initials: N
authors.lastname: Miyano-Kurosaki
authors.forename: Kahoko
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Kunitada
authors.initials: K
authors.lastname: Shimotohno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takaku
mesh_terms: D016367:Baculoviridae / Q000235:genetics*; D005822:Genetic Vectors; D016174:Hepacivirus / Q000235:genetics* / Q000502:physiology; D034622:RNA Interference; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D014758:Viral Core Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D014779:Virus Replication
chemical_list: D022661:RNA, Untranslated; D012367:RNA, Viral; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus