Analysis of PPAR alpha function in human kidney cell line using siRNA.
The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. PPARalpha is mainly expressed in the liver, kidney, heart and muscle. PPARalpha activates fatty acid catabolism, stimulates gluconeogenesis and ketone body synthesis and is involved in the control of lipoprotein assembly. Although PPARalpha is well characterized in the liver, its physiological function is unknown in the kidney. To investigate the intimate function of PPARalpha in the kidney, we analyzed the target gene expression in human metastatic renal cell carcinoma cell line, Caki-1, using small interfering RNA (siRNA) against PPARalpha and real-time RT-PCR methods. We found that some selected genes (long-chain fatty-acid-CoA ligase (FACL1), carnitine palmitoyltransferase 1A (CPT1A), adipose differentiation-related protein (ADRP) and aquaporin 3 (AQP3)) were down-regulated by PPARalpha siRNA. These results suggest that PPARalpha regulates fatty acid metabolism and body water homeostasis in this cell line.
Tachibana
Keisuke
K
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Anzai
Naohiko
N
Ueda
Chihiro
C
Katayama
Tatsuya
T
Kirino
Takayoshi
T
Takahashi
Rika
R
Yamasaki
Daisuke
D
Ishimoto
Kenji
K
Tanaka
Toshiya
T
Hamakubo
Takao
T
Ueda
Yukihiko
Y
Arai
Hiroyuki
H
Sakai
Juro
J
Kodama
Tatsuhiko
T
Doi
Takefumi
T
D001834:Body Water / Q000378:metabolism; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D047493:PPAR alpha / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D034741:RNA, Small Interfering
D005227:Fatty Acids; D047493:PPAR alpha; D034741:RNA, Small Interfering