title: Ribonucleopeptides recognize the phosphotyrosine residue.
abstract: Tools for selective recognition and sensing of kinase-catalyzed phosphorylation of Tyr or Ser/Thr residues located on a protein surface are essential for understanding signal transduction cascades of living cell. A stable complex of RNA and peptide (ribonucleopeptide) provides a new approach to tailor a receptor for small molecules. In vitro selection of an RNA-derived pool of ribonucleopeptide afforded a ribonucleopeptide receptor specific for phosphotyrosine (pTyr). Moreover, the ribonucleopeptide receptors are conveniently converted to fluorescent sensors for pTyr.
authors.affiliation: Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Susumu
authors.initials: S
authors.lastname: Yoshikawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Morii
mesh_terms: D001665:Binding Sites; D020650:Combinatorial Chemistry Techniques; D005456:Fluorescent Dyes / Q000737:chemistry*; D015694:Gene Products, rev / Q000737:chemistry; D008024:Ligands; D010455:Peptides / Q000737:chemistry*; D019000:Phosphotyrosine / Q000032:analysis*; D012313:RNA / Q000737:chemistry*; D012261:Ribonucleoproteins / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D015694:Gene Products, rev; D008024:Ligands; D010455:Peptides; D012261:Ribonucleoproteins; D019000:Phosphotyrosine; D012313:RNA