title: Substituent effects on the in situ activation of the double activated cross-linking reaction.
abstract: We have previously reported that the oligonucleotides (ODN) containing 2-amino-6-(1-ethylsulfinyl)vinyl purine derivatives (2) exhibit selective and efficient cross-linking to a cytidine at the target site under neutral conditions. In addition, bis-alkylsulfinyl derivative (3b) as the stable precursor of 2 showed the moderate reactivity. In this study, we have searched other bis-alkylsulfinyl precursors to achieve higher cross-linking ability, and found that acetamidoethyl and hydroxyethyl derivatives showed highly efficient and selective reactivity.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582 Japan.
authors.forename: Fumi
authors.initials: F
authors.lastname: Nagatsugi
authors.forename: Sayaka
authors.initials: S
authors.lastname: Mori
authors.forename: Md Monsur
authors.initials: MM
authors.lastname: Ali
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ogata
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sasaki
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry*; D003596:Cytosine / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011687:Purines / Q000737:chemistry*; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: C432203:2-amino-6-vinylpurine; D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D011687:Purines; D014753:Vinyl Compounds; D003596:Cytosine