Use of abasic site-containing DNA for electrochemical SNPs detection.
In combination with an electrode modified with an abasic site-containing DNA duplex, flavins selectively bind to thymine with a useful electrochemical response, and flavins could be successfully utilized for detection of thymine-related mutations. The abasic site provides hydrophobic microenvironments for the hydrogen bond-forming ligands can recognize the specific target base in the DNA duplex, which is detected by the difference in the electrochemical response.
Morita
Kotaro
K
Department of Chemistry, Graduate School of Science, Tohoku Univerisity, Aoba-ku, Sendai 980-8578, Japan.
Nishizawa
Seiichi
S
Teramae
Norio
N
D015374:Biosensing Techniques; D004252:DNA Mutational Analysis / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D005415:Flavins / Q000737:chemistry*; D006860:Hydrogen Bonding; D008024:Ligands; D008958:Models, Molecular; D020641:Polymorphism, Single Nucleotide; D012256:Riboflavin / Q000737:chemistry*; D013941:Thymine / Q000737:chemistry*
D015342:DNA Probes; D005415:Flavins; D008024:Ligands; C001922:lumiflavin; D013941:Thymine; D012256:Riboflavin