Effect of fly ash inhalation on biochemical and histomorphological changes in rat liver.
The effect of fly ash inhalation (4h daily, 5 days a week) for 28 days on the deposition of metal ions and histopathological changes in the liver and serum clinical enzymes has been studied. The results showed an increase in the concentration of metals such as cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), and lead (Pb) in the tissues of exposed rats. The level of metals varied from metal to metal and from organ to organ. Level of serum enzymes such as serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, and alkaline phosphatase were increased in fly ash exposed rats using whole body inhalation exposure as compared to sham controls. Histopathological studies of rat liver exposed to fly ash revealed infiltration of mononuclear cells in and around the portal triads, which seems to be laden with fly ash particles. Hepatocytes showed necrotic changes such as pyknotic nuclei, karyorrhexis, and karyolytic. These changes were more towards the centrolobular areas than the midzonal and periportal areas. These findings demonstrate that the toxic metals of inhaled fly ash in rats may get translocated into extrapulmonary organs, become deposited and hence may manifest their toxic effects on different tissues.
Mani
U
U
Inhalation Toxicology Laboratory, Industrial Toxicology Research Centre, PO Box 80, M. G. Marg, Lucknow 226 001, India. manitoxicology@yahoo.co.in
Prasad
A K
AK
Suresh Kumar
V
V
Lal
Kewal
K
Kanojia
R K
RK
Chaudhari
B P
BP
Murthy
R C
RC
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002244:Carbon / Q000633:toxicity*; D060729:Coal Ash; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D007220:Industrial Waste / Q000009:adverse effects*; D019570:Inhalation Exposure / Q000009:adverse effects*; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D019216:Metals, Heavy / Q000032:analysis / Q000378:metabolism; D009336:Necrosis / Q000139:chemically induced / Q000473:pathology; D052638:Particulate Matter / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
D060729:Coal Ash; D007220:Industrial Waste; D019216:Metals, Heavy; D052638:Particulate Matter; D002244:Carbon; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase