title: A role for Phospholipase D in Drosophila embryonic cellularization.
abstract: BACKGROUND
  Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically-translocated Golgi-derived vesicles.
  
  
  RESULTS
  We describe here involvement of the signaling enzyme Phospholipase D (Pld) in regulation of this developmental step. Functional analysis using gene targeting revealed that cellularization is hindered by the loss of Pld, resulting frequently in early embryonic developmental arrest. Mechanistically, chronic Pld deficiency causes abnormal Golgi structure and secretory vesicle trafficking.
  
  
  CONCLUSIONS
  Our results suggest that Pld functions to promote trafficking of Golgi-derived fusion-competent vesicles during cellularization.
authors.affiliation: Department of Pharmacology and Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794-5140, USA. mary.lalonde@uhmc.sunysb.edu <mary.lalonde@uhmc.sunysb.edu>
authors.forename: Mary
authors.initials: M
authors.lastname: LaLonde
authors.forename: Hilde
authors.initials: H
authors.lastname: Janssens
authors.forename: Suyong
authors.initials: S
authors.lastname: Yun
authors.forename: Juan
authors.initials: J
authors.lastname: Crosby
authors.forename: Olga
authors.initials: O
authors.lastname: Redina
authors.forename: Virginie
authors.initials: V
authors.lastname: Olive
authors.forename: Yelena M
authors.initials: YM
authors.lastname: Altshuller
authors.forename: Seok-Yong
authors.initials: SY
authors.lastname: Choi
authors.forename: Guangwei
authors.initials: G
authors.lastname: Du
authors.forename: J Peter
authors.initials: JP
authors.lastname: Gergen
authors.forename: Michael A
authors.initials: MA
authors.lastname: Frohman
mesh_terms: D000818:Animals; D019556:COS Cells; D002460:Cell Line; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C / Q000032:analysis; D002522:Chlorocebus aethiops; D048749:Cytokinesis; D022162:Cytoplasmic Vesicles / Q000737:chemistry / Q000201:enzymology / Q000502:physiology; D004331:Drosophila melanogaster / Q000166:cytology* / Q000196:embryology* / Q000235:genetics / Q000502:physiology; D004789:Enzyme Activation; D005260:Female; D006056:Golgi Apparatus / Q000502:physiology; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D008566:Membranes / Q000378:metabolism; D009154:Mutation; D010739:Phospholipase D / Q000235:genetics / Q000502:physiology*; D023121:RNA, Messenger, Stored / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection
chemical_list: C515538:CX3CL1 protein, human; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C; D008565:Membrane Proteins; D023121:RNA, Messenger, Stored; D010739:Phospholipase D