title: Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators.
abstract: DNA sequencing by synthesis (SBS) on a solid surface during polymerase reaction offers a paradigm to decipher DNA sequences. We report here the construction of such a DNA sequencing system using molecular engineering approaches. In this approach, four nucleotides (A, C, G, T) are modified as reversible terminators by attaching a cleavable fluorophore to the base and capping the 3'-OH group with a small chemically reversible moiety so that they are still recognized by DNA polymerase as substrates. We found that an allyl moiety can be used successfully as a linker to tether a fluorophore to 3'-O-allyl-modified nucleotides, forming chemically cleavable fluorescent nucleotide reversible terminators, 3'-O-allyl-dNTPs-allyl-fluorophore, for application in SBS. The fluorophore and the 3'-O-allyl group on a DNA extension product, which is generated by incorporating 3'-O-allyl-dNTPs-allyl-fluorophore in a polymerase reaction, are removed simultaneously in 30 s by Pd-catalyzed deallylation in aqueous buffer solution. This one-step dual-deallylation reaction thus allows the reinitiation of the polymerase reaction and increases the SBS efficiency. DNA templates consisting of homopolymer regions were accurately sequenced by using this class of fluorescent nucleotide analogues on a DNA chip and a four-color fluorescent scanner.
authors.affiliation: Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA. ju@c2b2.columbia.edu
authors.forename: Jingyue
authors.initials: J
authors.lastname: Ju
authors.forename: Dae Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Lanrong
authors.initials: L
authors.lastname: Bi
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Meng
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Bai
authors.forename: Zengmin
authors.initials: Z
authors.lastname: Li
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Li
authors.forename: Mong Sano
authors.initials: MS
authors.lastname: Marma
authors.forename: Shundi
authors.initials: S
authors.lastname: Shi
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: John R
authors.initials: JR
authors.lastname: Edwards
authors.forename: Aireen
authors.initials: A
authors.lastname: Romu
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Turro
mesh_terms: D001483:Base Sequence; D003116:Color; D004247:DNA / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D009711:Nucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005456:Fluorescent Dyes; D009711:Nucleotides; D004247:DNA