title: 'Genome gating'; polarized intranuclear trafficking of influenza virus RNPs.
abstract: Many viruses exploit cellular polarity to constrain the assembly and release of progeny virions to a desired surface. Influenza virus particles are released only from the apical surface of epithelial cells and this polarization is partly owing to specific targeting of the viral membrane proteins to the apical plasma membrane. The RNA genome of the virus is transcribed and replicated in the nucleus, necessitating nuclear export of the individual ribonucleoprotein (RNP) segments before they can be incorporated into budding virus particles. We show that the process of polarized virus assembly begins in the nucleus with the RNPs adopting a novel asymmetric distribution at the inner nuclear membrane prior to their export to the cytoplasm. The viral nucleoprotein, the major protein component of RNPs, displays the same polarized intranuclear distribution in the absence of other influenza virus components, suggesting the existence of a hitherto unrecognized polarity within the mammalian cell nucleus.
authors.affiliation: Department of Pathology, Division of Virology, University of Cambridge, Cambridge, UK.
authors.forename: Debra
authors.initials: D
authors.lastname: Elton
authors.forename: Maria Joao
authors.initials: MJ
authors.lastname: Amorim
authors.forename: Liz
authors.initials: L
authors.lastname: Medcalf
authors.forename: Paul
authors.initials: P
authors.lastname: Digard
mesh_terms: D000818:Animals; D002460:Cell Line; D016764:Cell Polarity; D006224:Cricetinae; D004847:Epithelial Cells / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D009685:Nuclear Envelope / Q000821:virology*; D012261:Ribonucleoproteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D019065:Virus Assembly / Q000502:physiology*
chemical_list: D012261:Ribonucleoproteins; D014764:Viral Proteins