title: Identification and measurement of illicit drugs and their metabolites in urban wastewater by liquid chromatography-tandem mass spectrometry.
abstract: Residues of illicit drugs and their metabolites that are excreted by humans may flow into and through wastewater treatment plants. The aim of this study was to develop a method for the determination of cocaine, amphetamines, morphine, cannabinoids, methadone, and some of their metabolites in wastewater. Composite 24-h samples from urban treatment plants were enriched with deuterated internal standards before solid-phase extraction. High-pressure liquid chromatography tandem mass spectrometry with multiple reaction monitoring was used for quantitation. Recoveries were generally higher than 80%, and limits of quantifications were in the low nanograms-per-liter range for untreated and treated wastewater. The overall variability of the method was lower than 10% for untreated and 5% for treated wastewater. The method was applied to wastewater samples coming from two treatment plants in Italy and Switzerland. Quantification ranges were found to be 0.2-1 microg/L for cocaine and its metabolite benzoylecgonine, 80-200 ng/L for morphine, 10 ng/L for 6-acetylmorphine, 60-90 ng/L for 11-nor-9-carboxy-Delta9-tetrahydrocannabinol, 10-90 ng/L for methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine, and lower than 20 ng/L for amphetamines. As previously reported for cocaine, this method could be useful to estimate and monitor drug consumption in the population in real time, helping social scientists and authorities to combat drug abuse.
authors.affiliation: Department of Environmental Health Sciences, Mario Negri Institute for Pharmacological Research, Via Eritrea 62, 20157 Milan, Italy. castiglioni@marionegri.it
authors.forename: Sara
authors.initials: S
authors.lastname: Castiglioni
authors.forename: Ettore
authors.initials: E
authors.lastname: Zuccato
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Crisci
authors.forename: Chiara
authors.initials: C
authors.lastname: Chiabrando
authors.forename: Roberto
authors.initials: R
authors.lastname: Fanelli
authors.forename: Renzo
authors.initials: R
authors.lastname: Bagnati
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D002947:Cities; D006801:Humans; D013287:Illicit Drugs / Q000378:metabolism* / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012722:Sewage / Q000032:analysis* / Q000737:chemistry; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D013287:Illicit Drugs; D012722:Sewage