Characterization of DNA hybridization on partially aminated diamond by aromatic compounds.
Here, we report a novel method of micropatterning oligonucleotides via aromatic groups as linkers on partially amino-terminated diamond and the inherence on subsequent hybridization. The covalent immobilization of probe oligonucleotides and characterization of immobilized probe oligonucleotides with carboxylic compounds were investigated by X-ray photoelectron spectroscopy (XPS). To confirm the effects of linker flexibility in a low amino group on diamond for probe oligonucleotides, three kinds of dicarboxylic compound--adipic acid, terephthalic acid, and trimesic acid--were used for immobilization of probe oligonucleotides, like linkers; and these oligonucleotides were hybridized with target oligonucleotides labeled with Cy 5 on the micropatterned diamond surface. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.
Yang
Jung-Hoon
JH
School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Song
Kwang-Soup
KS
Zhang
Guo-Jun
GJ
Degawa
Munenori
M
Sasaki
Yoshinori
Y
Ohdomari
Iwao
I
Kawarada
Hiroshi
H
D002232:Carbocyanines / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D018130:Diamond / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013052:Spectrometry, X-Ray Emission / Q000379:methods
D002232:Carbocyanines; D003432:Cross-Linking Reagents; D009841:Oligonucleotides; C085321:cyanine dye 5; D018130:Diamond