title: [Isolation and purification of puerarin from puerarin extractive by chelate complex chromatography].
abstract: Puerarin, an isoflavone compound, is an important ingredient in traditional Chinese medicine, which has the good medical effects on hypertension and angina. A new type of separation and purification method for isolating puerarin was developed using chelate complex chromatography. The stationary phase was prepared with silica gel containing 7% of Cu(OAc)2 in the chloroform. After the chloroform was evaporated, it was used as chelate complex chromatographic stationary phase to purify puerarin extractive. Pure puerarin was obtained when a mixture of chloroform and methanol (10:1, v/v) was used as eluent. In comparison with a common silica gel column, the chelate complex chromatographic column was more convenient and efficient. The purity and recovery of puerarin, and column capacity were improved 11%, 12% and 200%, respectively. Under the optimized experimental conditions, the purity of puerarin could be as high as with a recovery of 92%.
authors.affiliation: Engineering Research Center of Bio-Process of Ministry of Education, Hefei University of Technology, Hefei 230069, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Pan
authors.forename: Chuanxun
authors.initials: C
authors.lastname: Yuan
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Dai
mesh_terms: D002317:Cardiovascular Agents / Q000302:isolation & purification*; D002845:Chromatography; D056831:Coordination Complexes; D003300:Copper; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007529:Isoflavones / Q000302:isolation & purification*; D009942:Organometallic Compounds
chemical_list: D002317:Cardiovascular Agents; D056831:Coordination Complexes; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D009942:Organometallic Compounds; C015092:cupric acetate; D003300:Copper; C033607:puerarin