title: Screening of a glutamic acid-binding aptamer from arginine-modified DNA library.
abstract: A binder that is specific for a target will be applicable to detection of a variety of molecules such as bioactive substances, environmental contaminants and so on. Nucleic acid aptamers obtained by in vitro selection method are interesting and promising binders. To enhance the ability of nucleic acid aptamers, we prepared a combinatorial modified DNA library containing arginyl uracil base by using arginyl dUTP, then attempted screening of glutamic acid-binding aptamers from the library by in vitro selection method.
authors.affiliation: Department of Applied Chemistry, Fuclty of Engneering, Gunma University, 1-5-1 Tenjin-chou, Kiryu, Gunma 376-8515, Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Kazuomi
authors.initials: K
authors.lastname: Ohsawa
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kasamatsu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shoji
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sawai
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ozaki
mesh_terms: D052158:Aptamers, Peptide / Q000032:analysis* / Q000737:chemistry; D001120:Arginine / Q000031:analogs & derivatives* / Q000737:chemistry; D001665:Binding Sites; D003856:Deoxyuracil Nucleotides / Q000737:chemistry*; D015723:Gene Library; D018698:Glutamic Acid / Q000032:analysis*; D052156:SELEX Aptamer Technique
chemical_list: D052158:Aptamers, Peptide; D003856:Deoxyuracil Nucleotides; C520188:arginyl-5-((6-aminohexylcarbamoyl)methyl)-deoxyuridine triphosphate; D018698:Glutamic Acid; D001120:Arginine