[Influence of propyl-thiouracil-induced hypothyroidism on rat gingival mucosa].
This work aimed at verifying the influence of propyl-thiouracil (PTU)-induced thyroid hormone deficiency on gingival mucosa of young male rats, measuring total protein concentration, collagen content and DNA concentration as indices of cellular population. Fifty Sprague-Dawley rats were used. The animals were grouped in: PTU-treated (i.p. 10 mg/d) and control rats (C). The experience was maintained for a period of 10 weeks. Total protein content of gingival mucosa tissue was determined by the Lowry method; hydroxyprolin rate, as prototype amino acid of collagen, was determined using the Newman method, and DNA concentration was measured by Burton's methodology. The results showed decreased amounts of PTU-treated rats gingival total protein content (PTU= 41.23 +/- 24.05 vs. C= 63.36 +/- 18.05); no alterations were seen in hydroxyprolin concentration neither in DNA content of PTU treated rats, respectively (PTU= 2.18 +/- 1.48 vs. C= 2.29 +/- 1.51) and (PTU= 0.33 +/- 0.19 vs. C= 0.46 +/- 0.41). Thus, PTU treatment promotes a decrease in total protein content of rat gingival mucosa that may be interpreted as a decrease in protein synthesis induced by the hypothyroid condition, but with no alteration either in collagen or nucleic acid rates.
Douglas
Nicolás
N
Núcleo de Pesquisas Biológicas, Faculdade de Ciências Biológicas e da Saúde, Universidade Metodista de São Paulo. nicolasd@uol.com.br
Olmedo
Pedro
P
Douglas
Carlos Roberto
CR
Monte
Osmar
O
D000818:Animals; D013956:Antithyroid Agents; D003094:Collagen / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D003124:Colorimetry; D004247:DNA / Q000032:analysis; D004195:Disease Models, Animal; D005881:Gingiva / Q000737:chemistry*; D006909:Hydroxyproline / Q000032:analysis; D007037:Hypothyroidism / Q000139:chemically induced* / Q000378:metabolism; D008297:Male; D011441:Propylthiouracil; D011506:Proteins / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013053:Spectrophotometry; D013974:Thyroxine / Q000096:biosynthesis / Q000097:blood; D014284:Triiodothyronine / Q000096:biosynthesis / Q000097:blood
D013956:Antithyroid Agents; D011506:Proteins; D014284:Triiodothyronine; D011441:Propylthiouracil; D003094:Collagen; D004247:DNA; D013974:Thyroxine; D006909:Hydroxyproline