Stabilization of liposomes through enzymatic polymerization of DNA.
Combining supramolecular self-assembly of lipids with enzymatic triggered DNA interfacial polymerization allows construction of composite nanocapsules. Covalent grafting of oligonucleotides functionalizes the surface of liposomes. Subsequent addition of an enzyme called terminal deoxynucleotidyl transferase elongates the single-stranded DNA. The elongated DNA hybridizes, creating a random network. The short segments of double-stranded DNA provides a substrate for the Klenow fragment of E. coli DNA polymerase, which synthesizes a double-strand DNA, reinforcing the network. Alternate action of both enzymes leads to a three-dimensional network anchored on the liposome surface.
Ruysschaert
Tristan
T
Institut Pharmacologie et Biologie Structurale, UMR CNRS Université Paul Sabatier, 5089 Toulouse, France.
Paquereau
Laurent
L
Winterhalter
Mathias
M
Fournier
Didier
D
D000577:Amides / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004253:DNA Nucleotidylexotransferase / Q000737:chemistry*; D004256:DNA Polymerase I / Q000737:chemistry*; D004075:Diglycerides / Q000737:chemistry; D005456:Fluorescent Dyes; D008081:Liposomes / Q000737:chemistry*; D008301:Maleimides / Q000737:chemistry; D018613:Microscopy, Confocal; D009841:Oligonucleotides / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry
C516518:1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-n-(4-(p-maleimidophenyl)butyramide); D000577:Amides; D003432:Cross-Linking Reagents; D004075:Diglycerides; D005456:Fluorescent Dyes; D008081:Liposomes; D008301:Maleimides; D009841:Oligonucleotides; D010713:Phosphatidylcholines; D004247:DNA; D004253:DNA Nucleotidylexotransferase; D004256:DNA Polymerase I