title: Human osteoblast-like cell adhesion on titanium substrates covalently functionalized with synthetic peptides.
abstract: Biomaterials to be used for the production of endosseous devices in dental, orthopedic and maxillo-facial applications, might be designed to support, guide and enhance osteoblast adhesion. Cell recruitment onto biomaterial surface is a fundamental step within the complex process responsible for implant osseointegration; this process involves several proteins from the extra cellular matrix (ECM), cytoskeleton and cell membrane. A new strategy to improve endosseous implant integration is based on preparing biomimetic surfaces able to present adhesive factors to cells. Osteoblast adhesion takes place by at least two different mechanisms: the most investigated one implies the interaction with RGD sequences via cell-membrane integrin receptors; a further mechanism concerns the interaction between cell-membrane heparan sulfate proteoglycans and heparin-binding sites of ECM proteins. In the present study two different biomimetic surfaces were obtained by covalently grafting two adhesive peptides on oxidized titanium substrates after silanization: an RGD-containing peptide and a peptide mapped on human vitronectin. The two sequences are known to act via different adhesive mechanisms. The amount of human osteoblasts adhered onto peptide-enriched or not enriched titanium oxidized surfaces and the strength of cell binding were estimated, thus comparing the capacity of the bioactive substrates in promoting cell adhesion.
authors.affiliation: Department of Chemical Process Engineering, University of Padova, via Marzolo 9, 35131, Padova, Italy. andrea.bagno@unipd.it
authors.forename: Andrea
authors.initials: A
authors.lastname: Bagno
authors.forename: Alessandro
authors.initials: A
authors.lastname: Piovan
authors.forename: Monica
authors.initials: M
authors.lastname: Dettin
authors.forename: Alessia
authors.initials: A
authors.lastname: Chiarion
authors.forename: Paola
authors.initials: P
authors.lastname: Brun
authors.forename: Roberta
authors.initials: R
authors.lastname: Gambaretto
authors.forename: Giovanni
authors.initials: G
authors.lastname: Fontana
authors.forename: Carlo
authors.initials: C
authors.lastname: Di Bello
authors.forename: Giorgio
authors.initials: G
authors.lastname: Palù
authors.forename: Ignazio
authors.initials: I
authors.lastname: Castagliuolo
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000235:genetics; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005260:Female; D006801:Humans; D016023:Integrins / Q000235:genetics; D008297:Male; D008422:Materials Testing; D008875:Middle Aged; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology*; D011509:Proteoglycans / Q000235:genetics; D013499:Surface Properties; D014025:Titanium
chemical_list: D015815:Cell Adhesion Molecules; D020099:Coated Materials, Biocompatible; D016023:Integrins; D011509:Proteoglycans; C013065:cell aggregation factors; D014025:Titanium