On-line hyphenation of supercritical fluid extraction and two-dimensional high performance liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometer for the analysis of Ganoderma lucidum.
A novel on-line system combining supercritical fluid extraction (SFE) and two-dimensional high performance liquid chromatography (2D-HPLC) was developed. A trap column and two three-port valves were employed to couple SFE and 2D-HPLC system, which was composed of a CN column and a monolithic silica column, connected by a 10-port dual-position valve. The analytes extracted by supercritical CO2 were completely transferred to the 2D-HPLC system. After separation in two orthogonal modes, the eluents were delivered to APCI-tandem-MS for identification of the samples. In this way, sample preparation, separation, detection, and identification were integrated into an on-line system permitting analysis of the fruiting bodies of Ganoderma lucidum, and at least 73 components in the extract were resolved with calculated peak capacity of up to 1643.
Zhang
Jie
J
National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
Zhang
Lihua
L
Duan
Jicheng
J
Liang
Zhen
Z
Zhang
Weibing
W
Huo
Yushu
Y
Zhang
Yukui
Y
D000097:Acetonitriles / Q000032:analysis; D001274:Atmospheric Pressure; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D003201:Computers; D004867:Equipment Design; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D020940:Reishi / Q000378:metabolism*; D012815:Signal Processing, Computer-Assisted; D012822:Silicon Dioxide / Q000737:chemistry; D012984:Software; D013997:Time Factors
D000097:Acetonitriles; D012822:Silicon Dioxide; C032159:acetonitrile