Bowman-birk proteinase inhibitor confers heavy metal and multiple drug tolerance in yeast.
Cultured Coptis japonica cells show tolerance to various toxic compounds. By yeast functional screening of cadmium (Cd) plates with its cDNA library, we isolated a gene encoding Bowman-Birk proteinase inhibitor (CjBBI). The yeast transformant of CjBBI showed multiple tolerance to various drugs adding to Cd, and revealed reduced Cd accumulation in cells. Preferential organs for Cjbbi expression were aerial parts of intact plants, and the subcellular localization of CjBBI was shown, using its green fluorescent protein fusion, to be the apoplast. Induction of Cjbbi expression by Cd treatment suggested that CjBBI was responsible for the tolerance to Cd observed in C. japonica cells.
Shitan
Nobukazu
N
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, 611-0011 Japan.
Horiuchi
Ken-ichi
K
Sato
Fumihiko
F
Yazaki
Kazufumi
K
D001483:Base Sequence; D039323:Coptis / Q000502:physiology*; D017931:DNA Primers; D004361:Drug Tolerance; D019216:Metals, Heavy / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics; D014361:Trypsin Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*
D017931:DNA Primers; D019216:Metals, Heavy; D010940:Plant Proteins; D014361:Trypsin Inhibitors