Identification and Characterization of a Schizosaccharomyces pombe RNA Polymerase II Elongation Factor with Similarity to the Metazoan Transcription Factor ELL.
ELL family transcription factors activate the rate of transcript elongation by suppressing transient pausing by RNA polymerase II at many sites along the DNA. ELL-associated factors 1 and 2 (EAF1 and EAF2) bind stably to ELL family members and act as strong positive regulators of their transcription activities. Orthologs of ELL and EAF have been identified in metazoa, but it has been unclear whether such RNA polymerase II elongation factors are utilized in lower eukaryotes. Using bioinformatic and biochemical approaches, we have identified a new Schizosaccharomyces pombe RNA polymerase II elongation factor that is composed of two subunits designated SpELL and SpEAF, which share weak sequence similarity with members of the metazoan ELL and EAF families. Like mammalian ELL-EAF, SpELL-SpEAF stimulates RNA polymerase II transcription elongation and pyrophosphorolysis. In addition, like many yeast RNA polymerase II elongation factors, deletion of the SpELL gene renders S. pombe sensitive to the drug 6-azauracil. Finally, phylogenetic analyses suggest that the SpELL and SpEAF proteins are evolutionarily conserved in many fungi but not in Saccharomyces cerevisiae.
Banks
Charles A S
CA
Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
Kong
Stephanie E
SE
Spahr
Henrik
H
Florens
Laurence
L
Martin-Brown
Skylar
S
Washburn
Michael P
MP
Conaway
Joan W
JW
Mushegian
Arcady
A
Conaway
Ronald C
RC
D000595:Amino Acid Sequence; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000235:genetics* / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D018411:Spodoptera; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology*; D035602:Transcriptional Elongation Factors / Q000235:genetics* / Q000378:metabolism; D014498:Uracil / Q000031:analogs & derivatives / Q000494:pharmacology
D000963:Antimetabolites; C431383:EAF1 protein, human; C472771:EAF2 protein, human; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D035602:Transcriptional Elongation Factors; C518824:eaf1 protein, S pombe; C518823:ell1 protein, S pombe; D014498:Uracil; D012319:RNA Polymerase II; C100248:azauracil