Crystallization and preliminary X-ray analysis of RNA oligomers containing CUG repeats which induce type 1 myotonic dystrophy.
To reveal specific structure of CUG tandem repeats, which induce type 1 myotonic dystrophy, several fragments with different number of repeats have been synthesized, purified and crystallized. Their native PAGEs show that the structural stabilities of (CUG)6 and (CUG)7 are different, suggesting that when they adopt a hairpin form, the loops have different structures depending on the even and odd numbers of repeats. A preliminary structure of (CUG)6 crystal shows that the fragment forms a duplex similar to an A-form RNA.
Sato
Yoshiteru
Y
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.
Kimura
Kousei
K
Takénaka
Akio
A
D020029:Base Pairing; D001483:Base Sequence; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D009223:Myotonic Dystrophy / Q000235:genetics*; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D012313:RNA / Q000737:chemistry* / Q000235:genetics*; D055672:Static Electricity; D019680:Trinucleotide Repeat Expansion
D009692:Nucleic Acid Heteroduplexes; D012313:RNA