Regulation of eukaryotic DNA replication by proliferation associated protein, PA2G4, in vitro.
To search a novel factor possibly involved in the regulation of DNA replication for eukaryotic cells, we fractionated the Xenopus egg extract frequently used as a cell free DNA replication system, and obtained a single polypeptide of 50 KDa on SDS-polyacrylamide gel electrophoresis. Data of the amino acid sequence reduced from cloned gene of this protein revealed that this might be a Xenopus homolog of human PA2G4 that was known as a proliferation-associated protein or a cell cycle-regulated protein. Recombinant mouse PA2G4 protein also showed the inhibitory effect on the DNA replication reaction in Xenopus egg extract with the single-stranded DNA template. These results strongly suggest that PA2G4 may have an important role on the regulation of DNA replication in eukaryotic cells.
Izuta
Shunji
S
Department of Biological Science, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
Kitahara
Miki
M
Hamaguchi
Tomoko
T
D000818:Animals; D049109:Cell Proliferation; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D004268:DNA-Binding Proteins; D005057:Eukaryotic Cells / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism*; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins / Q000378:metabolism*; D014982:Xenopus laevis / Q000378:metabolism*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C521148:PA2G4 protein, Xenopus; C095938:Pa2g4 protein, mouse; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins; D004247:DNA