Catecholamine secretion from rat foetal adrenal chromaffin cells and hypoxia sensitivity.
The adrenal medulla chromaffin cells (AMCs) secrete catecholamines in response to various types of stress. We examined the hypoxia-sensitivity of catecholamine secretion by rat foetal chromaffin cells in which the innervation by the splanchnic nerve is not established. The experiments were performed in primary cultured cells from two different ages of foetuses (F15 and F19). Membrane potential of AMCs was monitored with the patch clamp technique, and the catecholamine secretion was detected by amperometry. We found that: (1) AMCs from F19 foetuses showed hypoxia-induced catecholamine release. (2) This hypoxia-induced secretion is produced by membrane depolarization generated by an inhibition of Ca(2+)-activated K(+) current [I (K(Ca))] current. (3) Chromaffin precursor cells from F15 foetuses secrete catecholamine. The quantal release is calcium-dependent, but the size of the quantum is reduced. (4) In the precursor cells, a hypoxia-induced membrane hyperpolarization is originated by an ATP-sensitive K(+) current [I (K(ATP))] activation. (5) During the prenatal period, at F15, the percentage of the total outward current for I (K(ATP)) and I (K(Ca)) was 50 and 29.5%, respectively, whereas at F19, I (K(ATP)) is reduced to 14%, and I (K(Ca)) became 64% of the total current. We conclude that before birth, the age-dependent hypoxia response of chromaffin cells is modulated by the functional activity of K(ATP) and K(Ca) channels.
Bournaud
R
R
Laboratoire de Neurobiologie Cellulaire et Moléculaire, Institut Alfred Fessard CNRS UPR 9040, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France. bournaud@nbcm.cnrs-gif.fr
Hidalgo
J
J
Yu
H
H
Girard
E
E
Shimahara
T
T
D000255:Adenosine Triphosphate / Q000502:physiology; D000313:Adrenal Medulla / Q000166:cytology* / Q000196:embryology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000378:metabolism*; D004594:Electrophysiology; D047109:Fetal Development / Q000502:physiology; D005333:Fetus / Q000166:cytology; D005865:Gestational Age; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology; D024681:Potassium Channels, Calcium-Activated / Q000037:antagonists & inhibitors / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
D002395:Catecholamines; D015221:Potassium Channels; D024681:Potassium Channels, Calcium-Activated; D000255:Adenosine Triphosphate; D002118:Calcium