title: Combined effect of betaine and trehalose on osmotic tolerance of Escherichia coli in mineral salts medium.
abstract: In mineral salts medium, supplementing with betaine in combination with increased production of endogenous osmoprotectant from a second copy of the trehalose biosynthetic genes (otsBA) improved growth of E. coli and increased the MIC for xylose, glucose, sodium lactate and NaCl. With these compounds, this combination was more effective than either betaine or trehalose alone. With succinate, this combination was no more effective than betaine alone. Neither approach improved tolerance to ethanol. A combination of betaine and increased trehalose may improve strain productivity for many bioproducts by promoting growth in the presence of high sugar concentrations.
authors.affiliation: Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA.
authors.forename: E N
authors.initials: EN
authors.lastname: Miller
authors.forename: L O
authors.initials: LO
authors.lastname: Ingram
mesh_terms: D001622:Betaine / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D009997:Osmotic Pressure / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology; D019354:Sodium Lactate / Q000494:pharmacology; D014199:Trehalose / Q000096:biosynthesis*; D014994:Xylose / Q000494:pharmacology
chemical_list: D003470:Culture Media; D001622:Betaine; D012965:Sodium Chloride; D014994:Xylose; D014199:Trehalose; D005947:Glucose; D019354:Sodium Lactate