Effects of the 2',4'-BNA modification on the sequence specificity of molecular beacons.
Molecular beacons (MBs) are stem-loop hairpin oligonucleotide probes with an internally quenched fluorophore. These probes recognize their targets with higher specificity than conventional linear probes. To further enhance sequence-specificity of MBs, we have designed and synthesized MBs having 2',4'-BNA modification. Thermodynamic analysis revealed that the MBs with the 2',4'-BNA-modified stem region have high sequence-specificity. In addition, the 2',4'-BNA modification in the loop region was also found to be efficient for discrimination of one base mismatch.
Fujii
Shiho
S
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Obika
Satoshi
S
Imanishi
Takeshi
T
D020137:Base Pair Mismatch; D001483:Base Sequence; D005456:Fluorescent Dyes / Q000737:chemistry*; D011905:Genes, ras; D006801:Humans; D009691:Nucleic Acid Denaturation; D015345:Oligonucleotide Probes / Q000737:chemistry*; D013816:Thermodynamics
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes