title: Isolation and characterization of a new bacterium capable of biotransforming cis -epoxysuccinic acid to D(-) -tartaric acid.
abstract: A bacterial strain 1-3 capable of enantioselectively hydrolyzing cis-epoxysuccinic acid to D(-)-tartaric acid was isolated from soil. Phenotypic characteristics, Biolog GN test and phylogenetic analysis of strain 1-3 indicated that it belongs to the genus Bordetella. cis-Epoxysuccinic acid could be used as a carbon source and inducer for production of cis-epoxysuccinic acid hydrolase. After the purification of the enzymatic biotransformed product, a colorless and scentless crystal was obtained, which was confirmed to be the tartaric acid with laevorotatory optical rotation by the (1)H nuclear magnetic resonance spectrum and optical rotation analysis.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Ma
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Jia
authors.forename: Lei
authors.initials: L
authors.lastname: Kai
authors.forename: Kangping
authors.initials: K
authors.lastname: Guo
authors.forename: Weifeng
authors.initials: W
authors.lastname: Zhao
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001711:Biotransformation; D001884:Bordetella / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D013386:Succinates / Q000737:chemistry / Q000378:metabolism*; D013644:Tartrates / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D013386:Succinates; D013644:Tartrates; C017290:2,3-epoxysuccinic acid; D002244:Carbon; D006867:Hydrolases; C029768:tartaric acid