Dose-related effects of direct hemoperfusion using a cytokine adsorbent column for the treatment of experimental endotoxemia.
OBJECTIVE
To clarify the dose-related effects of direct hemoperfusion using a cytokine adsorbent column (CTR) on the mortality and inflammatory responses to endotoxin-induced shock in rats.


METHODS
Prospective and randomized study.


METHODS
University research laboratory.


METHODS
Male Wistar rats.


METHODS
Forty-eight rats were injected intravenously with Escherichia coli endotoxin (15 mg/kg over 2 min), and then were randomly allocated to one of the following four groups (n = 12 per group): control group, treated without CTR for 120 min; quarter-dose treatment group, treated with CTR 0.25 ml for 120 min; half-dose treatment group, treated with CTR 0.50 ml; and full-dose treatment group, treated with CTR 1.0 ml.


RESULTS
Hemodynamics and arterial blood gases were recorded, and mortality and plasma cytokine concentrations were calculated for the 8-h observation period. The mortality rates 8 h after endotoxin injection were 92%, 58%, 42% and 17% for the control column, quarter-dose, half-dose, and full-dose CTR groups, respectively. The increases in plasma cytokine concentrations were smaller in the half-dose and full-dose CTR treatment groups than in the endotoxemic group.


CONCLUSIONS
The present study showed that CTR treatment dose-dependently decreased the mortality rate and inhibited inflammatory responses in vivo.
Taniguchi
Takumi
T
Department of Emergency and Critical Care Medicine, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, 920-8641, Kanazawa, Japan. takutaniyan@yahoo.co.jp
Kurita
Akihide
A
Mukawa
Chisui
C
Yamamoto
Ken
K
Inaba
Hideo
H
D000704:Analysis of Variance; D000818:Animals; D016207:Cytokines / Q000097:blood*; D019446:Endotoxemia / Q000097:blood / Q000628:therapy*; D006464:Hemoperfusion / Q000379:methods*; D015850:Interleukin-6; D008297:Male; D011446:Prospective Studies; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012772:Shock, Septic / Q000097:blood / Q000628:therapy*; D016019:Survival Analysis; D014409:Tumor Necrosis Factor-alpha
D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha