Neural crest-derived cells with stem cell features can be traced back to multiple lineages in the adult skin.
Given their accessibility, multipotent skin-derived cells might be useful for future cell replacement therapies. We describe the isolation of multipotent stem cell-like cells from the adult trunk skin of mice and humans that express the neural crest stem cell markers p75 and Sox10 and display extensive self-renewal capacity in sphere cultures. To determine the origin of these cells, we genetically mapped the fate of neural crest cells in face and trunk skin of mouse. In whisker follicles of the face, many mesenchymal structures are neural crest derived and appear to contain cells with sphere-forming potential. In the trunk skin, however, sphere-forming neural crest-derived cells are restricted to the glial and melanocyte lineages. Thus, self-renewing cells in the adult skin can be obtained from several neural crest derivatives, and these are of distinct nature in face and trunk skin. These findings are relevant for the design of therapeutic strategies because the potential of stem and progenitor cells in vivo likely depends on their nature and origin.
Wong
Christine E
CE
Department of Biology, Institute of Cell Biology, Swiss Federal Institute of Technology ETH Zurich, CH-8093 Zurich, Switzerland.
Paratore
Christian
C
Dours-Zimmermann
María T
MT
Rochat
Ariane
A
Pietri
Thomas
T
Suter
Ueli
U
Zimmermann
Dieter R
DR
Dufour
Sylvie
S
Thiery
Jean Paul
JP
Meijer
Dies
D
Beermann
Friedrich
F
Barrandon
Yann
Y
Sommer
Lukas
L
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000328:Adult; D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism; D005145:Face; D005260:Female; D005455:Fluorescent Antibody Technique; D018859:Hair Follicle / Q000166:cytology / Q000502:physiology; D006609:High Mobility Group Proteins / Q000378:metabolism; D006801:Humans; D008297:Male; D008544:Melanocytes / Q000166:cytology / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000502:physiology; D009432:Neural Crest / Q000166:cytology* / Q000502:physiology; D009457:Neuroglia / Q000166:cytology / Q000502:physiology; D055757:SOXE Transcription Factors; D012867:Skin / Q000166:cytology*; D014157:Transcription Factors / Q000378:metabolism
D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; C527923:SOX10 protein, human; D055757:SOXE Transcription Factors; C527924:Sox10 protein, mouse; D014157:Transcription Factors