Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear.
In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patientos ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.
Lee
Sun Joo
SJ
Department of Parasitology, School of Medicine, Pusan National University, Busan, Korea.
Jeong
Hae Jin
HJ
Lee
Ji Eun
JE
Lee
Jong Soo
JS
Xuan
Ying Hua
YH
Kong
Hyun-Hee
HH
Chung
Dong-Il
DI
Ock
Mee-Sun
MS
Yu
Hak Sun
HS
D000048:Acanthamoeba / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D015823:Acanthamoeba Keratitis / Q000188:drug therapy / Q000469:parasitology*; D000293:Adolescent; D000818:Animals; D000981:Antiprotozoal Agents / Q000008:administration & dosage; D001251:Astigmatism / Q000628:therapy; D001645:Biguanides / Q000008:administration & dosage; D002710:Chlorhexidine / Q000008:administration & dosage; D003261:Contact Lenses / Q000009:adverse effects*; D004272:DNA, Mitochondrial / Q000032:analysis; D016054:DNA, Protozoan / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004202:Disinfectants / Q000008:administration & dosage; D005260:Female; D006801:Humans; D017964:Itraconazole / Q000008:administration & dosage; D009216:Myopia / Q000628:therapy; D021261:Parasitic Sensitivity Tests; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
D000981:Antiprotozoal Agents; D001645:Biguanides; D004272:DNA, Mitochondrial; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D004202:Disinfectants; D012337:RNA, Ribosomal, 18S; D017964:Itraconazole; C031233:polihexanide; D002710:Chlorhexidine