title: [Detection of leptospira in the vitreous body of horses without ocular diseases and of horses with equine recurrent uveitis (ERU) using transmission-electron microscopy].
abstract: Equine recurrent uveitis (ERU) is caused by persistent intraocular leptospira, which appear to use the vitreous body as a refuge. The detection of leptospira in the vitreous body of horses with spontaneous ERU by histological methods has not yet been described. Thirty eight vitreous body samples from 36 horses with ERU (collected during vitrectomy), and 10 vitreous body samples obtained from 5 horses without ocular disease (control group) were examined by transmission electron microscopy. Prior to sample collection, 2 ml of a leptospira culture suspension were injected into the vitreous body of 2 eyes enucleated from horses of the control group. The detection of leptospira in samples, experimentally inoculated with these bacteria was uncomplicated; in vitreous body samples from horses with spontaneous ERU the detection was successful in only a few cases (3/38). The morphologically varying envelope of leptospira in vitreous body samples of horses which developed ERU spontaneously suggests the existence of a bacterial masquerade in vivo.
authors.affiliation: Klinik für Pferde der Ludwig-Maximilians-Universität München. g.niedermaier@pferd.vetmed.uni-muenchen.de
authors.forename: G
authors.initials: G
authors.lastname: Niedermaier
authors.forename: B
authors.initials: B
authors.lastname: Wollanke
authors.forename: R
authors.initials: R
authors.lastname: Hoffmann
authors.forename: S
authors.initials: S
authors.lastname: Brem
authors.forename: H
authors.initials: H
authors.lastname: Gerhards
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D015818:Eye Infections, Bacterial / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D006734:Horse Diseases / Q000175:diagnosis* / Q000382:microbiology; D006736:Horses; D007919:Leptospira / Q000302:isolation & purification / Q000648:ultrastructure*; D007922:Leptospirosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000379:methods / Q000662:veterinary; D012008:Recurrence; D016896:Treatment Outcome; D014605:Uveitis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D014821:Vitrectomy / Q000379:methods / Q000662:veterinary; D014822:Vitreous Body / Q000382:microbiology* / Q000601:surgery / Q000648:ultrastructure