title: Efficient generation of 2'-deoxyuridin-5-yl at 5'-(G/C)AA(X)U(X)U-3' (X = Br, I) sequences in duplex DNA under UV-irradiation.
abstract: A highly sequence-specific photoreaction of 5'-(G/C)AA(X)U(X)U-3' was found by using 450-base pair DNA fragments containing (X)U under UV-irradiation (X = Br, I). The molecular basis of this high photoreactivity was investigated in detail using synthetic octanucleotides. Based on the experimental data, the mechanism for the efficient photoreaction was proposed.
authors.affiliation: School of Biomedical Science, Tokyo Medical and Dental University, 2-3-10 Surugadai, Kanda, Chiyoda, Tokyo 101-0062, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Bando
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D001483:Base Sequence; D001966:Bromine / Q000737:chemistry*; D004247:DNA / Q000138:chemical synthesis* / Q000235:genetics / Q000528:radiation effects*; D007455:Iodine / Q000737:chemistry*; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000138:chemical synthesis* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014529:Uridine / Q000138:chemical synthesis*
chemical_list: D009692:Nucleic Acid Heteroduplexes; D004247:DNA; D007455:Iodine; D001966:Bromine; D014529:Uridine