Ex vivo perfusion with mitomycin C containing solution prolongs heart graft survival in rats.
Mitomycin C (MMC) is an alkylating agent which suppresses allogeneic T-cell responses. We analyzed the effect of graft perfusion with MMC on transplant survival. Hearts from Brown-Norway (BN) rats were perfused ex vivo with MMC-containing solution, stored and implanted into Lewis (LEW) rats. In order to analyze the in vivo effect of MMC, recipients received MMC posttransplantation or were pretreated with MMC-incubated donor-derived peripheral blood mononuclear cells (PBMCs). The results show that MMC-perfusion significantly prolongs graft survival. Treatment of recipients with MMC has no effect, whereas MMC-treated donor PBMCs injected into the recipient prolong graft survival. Our findings indicate that the targeted perfusion of donor hearts with MMC-containing solution protects the graft from rejection.
Wang
Daohu
D
Institute for Immunology, Department of Transplantation Immunology, University of Heidelberg, Heidelberg, Germany.
Kleist
Christian
C
Ehser
Sandra
S
Opelz
Gerhard
G
Terness
Peter
P
D000818:Animals; D017209:Apoptosis; D006085:Graft Survival / Q000187:drug effects*; D006321:Heart / Q000187:drug effects*; D016027:Heart Transplantation; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*; D010477:Perfusion; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D012996:Solutions / Q000494:pharmacology; D014019:Tissue Donors
D012996:Solutions; D016685:Mitomycin