Development of a liquid chromatography/electrospray ionization tandem mass spectrometric method for the determination of hydroxyl radical.
A rapid method combining liquid chromatography with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was developed for the determination of the hydroxyl radical (.OH). .OH generated via Fenton reaction was spin-trapped by 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and then analyzed by LC/ESI-MS/MS in multiple reaction monitoring (MRM) mode, using N-methyl-2-pyrrolidone (NMP) as the internal standard. The peak area ratio of DMPO-OH to NMP positively correlated with the concentration of .OH. The relative standard deviation (RSD) of the method was 1.13% (n = 8). The present method was successfully applied to evaluate the .OH scavenging capacity of several phenolic acids.
Yang
Fen
F
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Zhang
Ruiping
R
He
Jiuming
J
Abliz
Zeper
Z
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical / Q000032:analysis* / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D016877:Oxidants / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019097:Spin Trapping / Q000379:methods*
C045076:Fenton's reagent; D016877:Oxidants; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D007501:Iron