Synthesis and application of new amine-modified oligonucleotides using H-phosphonate chemistry.
Oligodeoxynucleotide probes tethered with amine linkers are commonly used for development of DNA based biosensor tools for studying gene expression and biological assays. However, the current preparation methods for synthesizing amine tethered probes often show defects during acid deprotection and during hydrophobic purification with the amine linker protecting groups. Here, we developed a prospective preparation method of using modified H-phosphonate chemistry to prepare amine tethered oligonucleotide probes. The present developed method will be helpful for preparing efficient amine tethered oligodeoxynucleotide probes that can be used in DNA biosensor applications.
Kamisetty
Nagendra Kumar
NK
Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan.
Pack
Seung Pil
SP
Nonogawa
Mitsuru
M
Devarayapalli
Kamakshaiah Charyulu
KC
Kodaki
Tsutomu
T
Makino
Keisuke
K
D000588:Amines / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000737:chemistry; D063065:Organophosphonates / Q000737:chemistry
D000588:Amines; D015345:Oligonucleotide Probes; D063065:Organophosphonates