title: Role of phosphoenolpyruvate in the NADP-isocitrate dehydrogenase and isocitrate lyase reaction in Escherichia coli.
abstract: Phosphoenolpyruvate inhibited Escherichia coli NADP-isocitrate dehydrogenase allosterically (Ki of 0.31 mM) and isocitrate lyase uncompetitively (Ki' of 0.893 mM). Phosphoenolpyruvate enhances the uncompetitive inhibition of isocitrate lyase by increasing isocitrate, which protects isocitrate dehydrogenase from the inhibition, and contributes to the control through the tricarboxylic acid cycle and glyoxylate shunt.
authors.affiliation: Department of Biochemistry, Aichi Medical University School of Medicine, Aichi 489-1195, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ogawa
authors.forename: Keiko
authors.initials: K
authors.lastname: Murakami
authors.forename: Hirotada
authors.initials: H
authors.lastname: Mori
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Ishii
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
authors.forename: Masataka
authors.initials: M
authors.lastname: Yoshin
mesh_terms: D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007521:Isocitrate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007522:Isocitrate Lyase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007523:Isocitrates / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D009249:NADP / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D007523:Isocitrates; D009249:NADP; D010728:Phosphoenolpyruvate; C034219:isocitric acid; D007521:Isocitrate Dehydrogenase; D007522:Isocitrate Lyase