Down-regulation of proteolytic complexes following EBV activation in BL cells.
In Burkitt's lymphoma cells, Epstein Barr virus (EBV) latency products interact with the ubiquitin-proteasome system to promote episomal maintenance and immunological evasion while the tripeptidylpeptidase II (TPPII) functions as an alternative protease. In the present study, we have examined the activities and levels of the proteasome and TPPII complex in Raji and in Akata cells after induction of EBV lytic cycle. The results show that the chymotrypsin-like and caspase-like activities of the proteasome were substantially reduced in Raji and Akata cells. Similarly, TPPII activity was diminished in both cell lines but was recovered in Akata cells at longer time after induction. Protein levels of the alpha/beta subunits of the 20S proteasome and TPPII concentration decreased to different extents after EBV activation, whereas the ubiquitin binding S6' subunit of the 19S regulatory complex increased three to fourfold along with the levels of ubiquitin-conjugates. Collectively, these observations demonstrate impairment of two major cellular proteolytic systems at the onset of EBV lytic infection.
Matusali
Giulia
G
Department of Public Health Sciences, University "La Sapienza", P. le A. Moro 5, 00185 Rome, Italy.
De Leo
Alessandra
A
Gavioli
Riccardo
R
Bertelli
Laura
L
Di Renzo
Livia
L
Mattia
Elena
E
D002051:Burkitt Lymphoma / Q000201:enzymology*; D002460:Cell Line; D015536:Down-Regulation; D004854:Herpesvirus 4, Human / Q000502:physiology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D014775:Virus Activation
D010447:Peptide Hydrolases