title: Interleukin-10 gene transfer: prevention of multiple organ injury in a murine cecal ligation and puncture model of sepsis.
abstract: BACKGROUND
  The aim of this study was to determine the effect of immunoregulatory cytokine interleukin-10 (IL-10) gene therapy on multiple organ injury (MOI) induced by a cecal ligation and puncture (CLP) model of sepsis in mice.
  
  
  METHODS
  Male Balb/c mice subjected to CLP were treated with either an hIL-10-carrying vector or an empty control vector. We assessed the degree of lung, liver, and kidney tissue destruction biochemically by measuring myeloperoxidase (MPO) and malondialdehyde (MDA) activity. Histologic assessments were based on neutrophil infiltration in lung and liver tissue. IL-10 protein expression was examined immunohistochemically, and ultrastructural changes in the liver were studied by transmission electron microscopy. We analyzed the expression of tumor necrosis factor-alpha (TNFalpha) mRNA by reverse transcription polymerase chain reaction 3, 8, and 24 hours after CLP in all organs.
  
  
  RESULTS
  Organ damage was significantly reduced by hIL-10 gene transfer, which was associated at the tissue level with reduced MPO activity in the liver, lung, and kidney and decreased leukocyte sequestration and MDA formation in the lung. The liver MDA was not significantly higher in the hIL-10 gene therapy group than in the controls and seemed not to be affected by hIL-10 gene transfer. The reduced portal tract neutrophilic infiltration and preserved ultrastructure of the hepatocytes also showed that tissue function was not impaired. The lung and kidney TNFalpha mRNA expression was suppressed markedly in the hIL-10 gene therapy group, but liver TNFalpha mRNA expression varied over time.
  
  
  CONCLUSIONS
  These findings showed that IL-10 gene therapy significantly attenuated sepsis-induced MOI.
authors.affiliation: Department of General Surgery, Pamukkale University, School of Medicine, Kinikli; Kampüsü A408, 2000, Denizli, Turkey. bkabay@pau.edu.tr
authors.forename: Burhan
authors.initials: B
authors.lastname: Kabay
authors.forename: Cetin
authors.initials: C
authors.lastname: Kocaefe
authors.forename: Atac
authors.initials: A
authors.lastname: Baykal
authors.forename: Hilmi
authors.initials: H
authors.lastname: Ozden
authors.forename: Cengiz
authors.initials: C
authors.lastname: Baycu
authors.forename: Zafer
authors.initials: Z
authors.lastname: Oner
authors.forename: Meral
authors.initials: M
authors.lastname: Ozgüç
authors.forename: Iskender
authors.initials: I
authors.lastname: Sayek
mesh_terms: D000818:Animals; D002432:Cecum / Q000601:surgery*; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000008:administration & dosage / Q000032:analysis / Q000627:therapeutic use*; D008026:Ligation; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009102:Multiple Organ Failure / Q000209:etiology / Q000517:prevention & control*; D020556:Neutrophil Infiltration; D009195:Peroxidase / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018805:Sepsis / Q000150:complications / Q000628:therapy*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D014950:Wounds, Penetrating
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D008315:Malondialdehyde; D009195:Peroxidase