The expression of phosphatidic acid phosphatase 2a, which hydrolyzes lipids to generate diacylglycerol, is regulated by p73, a member of the p53 family.
p73, a p53-related gene, is essential for a development of animals, while p53 is important for tumor formation. And little is known about the target genes specifically regulated by p73. Identifying the specific targets of p73 is important to understand the physiological roles of p73. To identify the genes specifically regulated by p73, we conducted serial analysis of gene expression to quantitatively evaluate messenger RNA populations. We found that the gene for phosphatidic acid phosphatase 2a (PAP2a), an enzyme that hydrolyzes lipids to generate diacylglycerol, was specifically upregulated by ectopic production of p73beta. The promoter region of this gene contains an element that is functionally responsive to p73beta. And the quantity of PAP2a protein was upregulated by ectopic production of p73beta. These results suggest that the expression of PAP2a is directly regulated by p73.
Ishida
Takeshi
T
Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto, Japan.
Iwai
Akio
A
Hijikata
Makoto
M
Shimotohno
Kunitada
K
D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004075:Diglycerides / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D006868:Hydrolysis; D050356:Lipid Metabolism / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism*; D010711:Phosphatidate Phosphatase / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
D004268:DNA-Binding Proteins; D004075:Diglycerides; D009687:Nuclear Proteins; D025521:Tumor Suppressor Proteins; C112948:lipid phosphate phosphatase; D010711:Phosphatidate Phosphatase