title: Characterization of volatile constituents of Scaligeria tripartita and studies on the antifungal activity against phytopathogenic fungi.
abstract: The chemical composition of the essential oils obtained from stems and leaves, fruits and roots of Scaligeria tripartita oil was analyzed by gas chromatography-mass spectrometry. A total of 38 compounds were identified ranging 89-94% of the oil samples. Geijerene was found as a main compound in the oils of the stems and leaves (37%) and fruits (55%), whereas epoxypseudoisoeugenol angelate (37%) was found as a main compound in the root oil. Oils were subsequently evaluated for their antimalarial, antimicrobial against human pathogenic bacteria or fungi and antifungal activities against plant pathogens. Antifungal activity of Scaligeria oils was observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides using the direct overlay bioautography assay. Chemotaxonomically important pure compounds indicated in the bioautography assay were subsequently evaluated in a 96-well microdilution broth assay. The performance of overpressured layer chromatography (OPLC) and TLC for the analysis of Scaligeria essential oils was also compared.
authors.affiliation: USDA-ARS-NPURU, The University of Mississippi, University, MS 38677, USA.
authors.forename: Nurhayat
authors.initials: N
authors.lastname: Tabanca
authors.forename: Betul
authors.initials: B
authors.lastname: Demirci
authors.forename: K Husnu Can
authors.initials: KH
authors.lastname: Baser
authors.forename: Emil
authors.initials: E
authors.lastname: Mincsovics
authors.forename: Shabana I
authors.initials: SI
authors.lastname: Khan
authors.forename: Melissa R
authors.initials: MR
authors.lastname: Jacob
authors.forename: David E
authors.initials: DE
authors.lastname: Wedge
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D019661:Apiaceae / Q000737:chemistry* / Q000382:microbiology; D002855:Chromatography, Thin Layer; D005658:Fungi / Q000187:drug effects* / Q000472:pathogenicity; D008401:Gas Chromatography-Mass Spectrometry; D008826:Microbial Sensitivity Tests; D014835:Volatilization
chemical_list: D000935:Antifungal Agents