title: Transcriptional profiles of chicken embryo cell cultures following infection with infectious bursal disease virus.
abstract: Infectious bursal disease virus (IBDV) is the causative agent of infectious bursal disease in chickens and causes a significant economic loss for the poultry industry. Little is understood about the mechanism involved in the host responses to IBDV infection. For better understanding the IBDV-host interaction, we measured steady-state levels of transcripts from 28 cellular genes of chicken embryo (CE) cell cultures infected with IBDV vaccine stain Bursine-2 during a 7-day infection course by use of the quantitative real-time RT-PCR SYBR green method. Of the genes tested, 21 genes (IRF-1, IFN 1-2 promoter, IFNAR-1, IRF-10, IFN-gamma, 2',5'-OAS, IAP-1, caspase 8, TRAIL-like, STAT-3, IL-6, IL-8, MIP-3 alpha, MHC-I, MHC-II, TVB, GLVR-1, OTF, IL-13R alpha, ST3GAL-VI and PGK) showed an increased expression. The remaining seven genes (IFNAR-2, IFN-alpha, NF-kappaB subunit p65, BLRcp38, DDX1, G6PDH and UB) showed a constant expression or only slight alteration. Apparently, the host genes involved in pro-inflammatory response and apoptosis, interferon-regulated proteins, and the cellular immune response were affected by IBDV infection, indicating involvement in the complex signaling pathways of host responses to the infection. This study thus contributes to the understanding of the pathogenesis of IBD and provides an insight into the virus-host interaction.
authors.affiliation: Department of Poultry, Fish and Fur Animals, Danish Institute for Food and Veterinary Research, Aarhus, Denmark. yil@dfvf.dk
authors.forename: Y P
authors.initials: YP
authors.lastname: Li
authors.forename: K J
authors.initials: KJ
authors.lastname: Handberg
authors.forename: H R
authors.initials: HR
authors.lastname: Juul-Madsen
authors.forename: M F
authors.initials: MF
authors.lastname: Zhang
authors.forename: P H
authors.initials: PH
authors.lastname: Jørgensen
mesh_terms: D000818:Animals; D017209:Apoptosis; D018175:Birnaviridae Infections / Q000235:genetics* / Q000473:pathology / Q000662:veterinary; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002642:Chick Embryo; D016207:Cytokines / Q000235:genetics; D017931:DNA Primers; D006863:Hydrogen-Ion Concentration; D007243:Infectious bursal disease virus / Q000235:genetics* / Q000302:isolation & purification; D008285:Major Histocompatibility Complex; D011201:Poultry Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D019562:Viral Load; D014764:Viral Proteins / Q000235:genetics
chemical_list: D016207:Cytokines; D017931:DNA Primers; D012367:RNA, Viral; D014764:Viral Proteins