Negative-strand hepatitis C virus (HCV) RNA in peripheral blood mononuclear cells from anti-HCV-positive/HIV-infected women.
BACKGROUND
Hepatitis C virus (HCV) has been reported to replicate in peripheral blood mononuclear cells (PBMCs), particularly in patients coinfected with HCV and human immunodeficiency virus (HIV). However, there are limited data regarding the prevalence of and the factors associated with extrahepatic replication.


METHODS
The presence of negative-strand HCV RNA in PBMCs was evaluated by a strand-specific assay for 144 anti-HCV-positive/HIV-infected women enrolled in the Women's Interagency HIV Study. One to 5 PBMC samples obtained from each woman were tested. Multivariate analyses were used to assess for associations with the clinical and demographic characteristics of the women.


RESULTS
Negative-strand HCV RNA was detected in 78 (25%) of 315 specimens, and, for 61 women (42%), > or = 1 specimen was found to have positive results. The presence of negative-strand HCV RNA in PBMCs was significantly positively associated with an HCV RNA plasma level of > or = 6.75 log copies/mL (P=.04) and consumption of > or = 7 alcoholic drinks per week (P=.02). It was also negatively associated with injection drug use occurring in the past 6 months (P=.03). A negative association with a CD4+ CD38+ DR+ cell percentage of > 10% and a positive association with acquired immunodeficiency syndrome were borderline significant (P=.05).


CONCLUSIONS
HCV replication in PBMCs is common among HIV-coinfected women and appears to be a dynamic process related to lifestyle, virologic, and immunologic factors.
Laskus
Tomasz
T
Department of Medicine, St. Joseph's Hospital and Medical Center, Phoenix, AZ 85013, USA. Tomasz.Laskus@chw.edu
Operskalski
Eva A
EA
Radkowski
Marek
M
Wilkinson
Jeffrey
J
Mack
Wendy J
WJ
deGiacomo
Marina
M
Al-Harthi
Lena
L
Chen
Zhi
Z
Xu
Jiaao
J
Kovacs
Andrea
A
D015331:Cohort Studies; D005260:Female; D015658:HIV Infections / Q000150:complications* / Q000821:virology; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification
D012367:RNA, Viral