Analytical validation of commercially available methods for acute phase proteins quantification in pigs.
The aim of this study was to validate commercially available methods for porcine haptoglobin (Hp), C-reactive protein (CRP), serum amyloid A (SAA) and major acute phase protein (Pig-MAP) determinations. Intra and inter assay coefficients of variation (CVs) were lower than 20% in all cases with exception of inter assay CVs for CRP and Pig-MAP assays with samples of low acute phase proteins concentration, and for SAA assay at any acute phase proteins concentration. All methods showed good linearity and detection limits were low enough to detect APPs levels in healthy animals. Hp and SAA were very affected by haemolysis. Lipaemia influenced mainly on SAA determination. Over 15-fold increase was observed in CRP and SAA concentrations after artificially induced inflammation by a single subcutaneous dose of turpentine, whereas Hp and Pig-MAP increased less than 5-fold.
Tecles
F
F
Animal Medicine and Surgery Department, Veterinary School, University of Murcia, Campus de Espinardo, s/n 30100 Espinardo, Murcia, Spain.
Fuentes
P
P
Martínez Subiela
S
S
Parra
M D
MD
Muñoz
A
A
Cerón
J J
JJ
D000209:Acute-Phase Proteins / Q000378:metabolism*; D000818:Animals; D002097:C-Reactive Protein / Q000378:metabolism*; D006242:Haptoglobins / Q000378:metabolism*; D015203:Reproducibility of Results; D000685:Serum Amyloid A Protein / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000378:metabolism
D000209:Acute-Phase Proteins; D006242:Haptoglobins; D000685:Serum Amyloid A Protein; D002097:C-Reactive Protein