title: The number of G residues in the Bacillus subtilis pyrG initially transcribed region governs reiterative transcription-mediated regulation.
abstract: Derepression of pyrG expression in Bacillus subtilis involves CTP-sensitive reiterative transcription, which introduces up to 11 extra G residues at the 5' ends of pyrG transcripts. Insertion of three or more additional Gs at the 5' end of the pyrG initially transcribed region abolished reiterative transcription and caused constitutive expression.
authors.affiliation: Department of Biochemistry, University of Illinois, Urbana, IL 61801, USA.
authors.forename: Alexander K W
authors.initials: AK
authors.lastname: Elsholz
authors.forename: Casper Møller
authors.initials: CM
authors.lastname: Jørgensen
authors.forename: Robert L
authors.initials: RL
authors.lastname: Switzer
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D019731:Carbon-Nitrogen Ligases / Q000235:genetics* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D014158:Transcription, Genetic
chemical_list: D019731:Carbon-Nitrogen Ligases; C019951:CTP synthetase