Development of a novel DNA sensing system using DNA aptamer that inhibits enzymatic activity 2.
We have developed novel DNA sensor system using the DNA aptamers for thrombin. We modified original thrombin aptamer by addition of the target DNA complementary strands to the 5' end or 3' end of aptamer. Inhibitory activity of this modified aptamer increased in the presence of target DNA. We were able to detect the target DNA by measuring thrombin activity using this aptamer without the separation of bound and free separation.
Ikebukuro
Kazunori
K
Department of Biotechnology & Life Science, Tokyo University of Agriculture & Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Yoshida
Wataru
W
Noma
Takahisa
T
Sode
Koji
K
D052157:Aptamers, Nucleotide / Q000494:pharmacology*; D015374:Biosensing Techniques / Q000379:methods*; D001777:Blood Coagulation / Q000187:drug effects; D004247:DNA / Q000032:analysis*; D008954:Models, Biological; D013917:Thrombin / Q000037:antagonists & inhibitors*
D052157:Aptamers, Nucleotide; C081676:thrombin aptamer; D004247:DNA; D013917:Thrombin