title: Herbicide resistance of transgenic rice plants expressing human CYP1A1.
abstract: Cytochrome P450 monooxygenases (P450s) metabolize herbicides to produce mainly non-phytotoxic metabolites. Although rice plants endogenously express multiple P450 enzymes, transgenic plants expressing other P450 isoforms might show improved herbicide resistance or reduce herbicide residues. Mammalian P450s metabolizing xenobiotics are reported to show a broad and overlapping substrate specificity towards lipophilic foreign chemicals, including herbicides. These P450s are ideal for enhancing xenobiotic metabolism in plants. A human P450, CYP1A1, metabolizes various herbicides with different structures and modes of herbicide action. We introduced human CYP1A1 into rice plants, and the transgenic rice plants showed broad cross-resistance towards various herbicides and metabolized them. The introduced CYP1A1 enhanced the metabolism of chlorotoluron and norflurazon. The herbicides were metabolized more rapidly in the transgenic rice plants than in non-transgenic controls. Transgenic rice plants expressing P450 might be useful for reducing concentrations of various chemicals in the environment.
authors.affiliation: Plant Biotechnology Department, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan. hiwak@affrc.go.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawahigashi
authors.forename: Sakiko
authors.initials: S
authors.lastname: Hirose
authors.forename: Hideo
authors.initials: H
authors.lastname: Ohkawa
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Ohkawa
mesh_terms: D001673:Biodegradation, Environmental; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000378:metabolism*; D053209:Herbicide Resistance / Q000235:genetics*; D006801:Humans; D012275:Oryza / Q000187:drug effects* / Q000196:embryology / Q000235:genetics*; D030821:Plants, Genetically Modified; D015262:Xenobiotics / Q000378:metabolism / Q000494:pharmacology
chemical_list: D015262:Xenobiotics; D019363:Cytochrome P-450 CYP1A1