Gene families encoding isoforms of two major sesame seed storage proteins, 11S globulin and 2S albumin.
Sesame (Sesamum indicum L.) seed has been recognized as a nutritional protein source owing to its richness in methionine. Storage proteins have been implicated in allergenic responses to sesame consumption. Two abundant storage proteins, 11S globulin and 2S albumin, constitute 60-70 and 15-25% of total sesame proteins, respectively. Two gene families separately encoding four 11S globulin and three 2S albumin isoforms were identified in a database search of 3328 expressed sequence tag (EST) sequences from maturing sesame seeds. Full-length cDNA sequences derived from these two gene families were completed by PCR using a maturing sesame cDNA library as the template. The amino acid compositions of these deduced storage proteins revealed that the richness in methionine is attributed mainly to two 2S albumin isoforms and partly to one 11S globulin isoform. The presence of four 11S globulin and three 2S albumin isoforms resolved in SDS-PAGE was confirmed by MALDI-MS analyses. The abundance of these isoforms was in accord with the occurrence frequency of their EST sequences in the database. A comprehensive understanding of these storage proteins at the molecular level may also facilitate the identification of allergens in crude sesame products that have caused severe allergic reactions increasingly reported in the past decade.
Hsiao
Eric S L
ES
Graduate Institute of Biotechnology and Department of Chemistry, National Chung Hsing University, Taichung, Taiwan.
Lin
Li-Jen
LJ
Li
Feng-Yin
FY
Wang
Miki M C
MM
Liao
Ming-Yuan
MY
Tzen
Jason T C
JT
D000418:Albumins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000737:chemistry; D018744:DNA, Plant / Q000737:chemistry; D015723:Gene Library; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics*; D012639:Seeds / Q000737:chemistry*; D016415:Sequence Alignment; D031685:Sesamum / Q000737:chemistry* / Q000235:genetics*
D000418:Albumins; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D020033:Protein Isoforms