title: Recognition sites of glycine tRNA for glycyl-tRNA synthetase from hyperthermophilic archaeon, Aeropyrum pernix K1.
abstract: To elucidate the tRNA recognition sites of glycine tRNA from an extreme thermophilic and aerobic archaeon, Aeropyrum pernix K1, we examined glycylation activities using in vitro mutant glycine tRNA transcripts and recombinant A. pernix glycyl-tRNA synthetase. The recognition nucleotides were determined to be C35 and C36 of anticodon, C2-G71 and G3-C70 base-pairs of acceptor stem. However, discriminator base A73 was not recognized by glycyl-tRNA synthetase.
authors.affiliation: Graduate School of Science and Technology, Yamagata University, Yamagata 990-8560, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Okamoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuno
authors.forename: Tsunemi
authors.initials: T
authors.lastname: Hasegawa
mesh_terms: D041522:Aeropyrum / Q000201:enzymology*; D006032:Glycine-tRNA Ligase / Q000378:metabolism*; D009154:Mutation; D012353:RNA, Transfer, Gly / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D012353:RNA, Transfer, Gly; D006032:Glycine-tRNA Ligase