Kinetic of biomarker responses in juveniles of the fish Sparus aurata exposed to contaminated sediments.
Sediments in the National Park of the Atlantic Islands (Galicia, Spain) were affected by the spill of the tanker Prestige (November, 2002) and still present high levels of Polycyclic aromatic hydrocarbons. The adverse effects associated with the contaminants in sediments were tested using a chronic bioassay, exposing juveniles of the fish Sparus aurata (seabream). A toxicokinetic approach is proposed to evaluate sediment quality by linking chemical and ecotoxicological data along the time. Sediment samples were physicochemically characterized and the concentration of contaminants (Polycyclic aromatic hydrocarbons - PAHs - and metals) was measured. Fishes were exposed to contaminated sediments, and samples from different tissues were collected every 15 days throughout the 60 days that lasted the experiment. A biomarker of exposure (ethoxyresorufin O-deethylase activity - EROD activity) and a biomarker of effect (histopathology) were analyzed during the exposure period. Results show a relationship between the biomarkers and the concentrations in sediments of polycyclic aromatic hydrocarbons-PAHs. Besides, the toxicokinetic approach links biomarkers response providing information about the relationship between the detoxification process and the damages observed in the different tissues. The frequency of the histological damage is highest when the EROD activity slightly decreases in accordance with the mechanism of detoxification of this enzymatic system against PAHs and other organic contaminants.
Morales-Caselles
Carmen
C
Consejo Superior de Investigaciones Científicas (CSIC), Instituto de Ciencias Marinas de Andalucía (ICMAN), Polígono Río San Pedro s/n, 11510, Puerto Real, Cádiz, Spain.
Jiménez-Tenorio
Natalia
N
Riba
Inmaculada
I
Sarasquete
Carmen
C
DelValls
T Angel
TA
D000059:Accidents; D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods*; D019015:Geologic Sediments / Q000032:analysis*; D010578:Petroleum / Q000009:adverse effects; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D021541:Sea Bream; D012767:Ships; D013030:Spain; D014876:Water Pollution / Q000032:analysis*
D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D019363:Cytochrome P-450 CYP1A1