Anti-inflammatory effects of taurine derivatives (taurine chloramine, taurine bromamine, and taurolidine) are mediated by different mechanisms.
In this study, in an animal model of zymosan-induced peritonitis we have tested anti-inflammatory properties of Taurolidine (TRD), a synthetic derivative of taurine. In vitro, the effect of TRD and HOCl treated TRD on peritoneal macrophages was compared with that of TauCl. We report that locally administered TRD (Taurolin) shows strong anti-inflammatory properties. TRD inhibits vascular permeability increased by inflammatory stimuli; it also significantly attenuates the influx of neutrophils into the peritoneal cavity, as well as the production of pro-inflammatory cytokines (TNF-alpha, IL-6) by peritoneal exudate cells. Chlorination of TRD resulted in the formation of chloramine (TRD-Cl), as confirmed by characteristic UV spectra. Both TRD and TRD-Cl, more effectively than TauCl, inhibited the production of IL-6 by stimulated macrophages. The effect was not dependent on its well-known anti-endotoxin activity since TRD inhibited cytokine production by macrophages stimulated with either LPS or IFN-gamma. Finally, we report that anti-inflammatory activities of TRD and taurine haloamines are mediated by different mechanisms. TRD, in contrast to TauCl and TauBr, does not induce expression of HO-1, a stress inducible enzyme with strong anti-inflammatory properties.
Marcinkiewicz
Janusz
J
Department of Immunology, Jagiellonian University Medical College, Krakow, Poland. mmmarcin@cyf-kr.edu.pl
Kurnyta
Maria
M
Biedroń
Rafał
R
Bobek
Małgorzata
M
Kontny
Ewa
E
Maśliński
Włodzimierz
W
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D002478:Cells, Cultured; D002700:Chloramines / Q000378:metabolism; D004790:Enzyme Induction; D051547:Heme Oxygenase-1 / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy; D007371:Interferon-gamma / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D010538:Peritonitis / Q000139:chemically induced / Q000188:drug therapy; D013654:Taurine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D013829:Thiadiazines / Q000494:pharmacology / Q000627:therapeutic use; D015054:Zymosan / Q000633:toxicity
D000893:Anti-Inflammatory Agents; D002700:Chloramines; D015850:Interleukin-6; D013829:Thiadiazines; D013654:Taurine; D007371:Interferon-gamma; C012566:taurolidine; D015054:Zymosan; D051547:Heme Oxygenase-1; C030816:chloramine