Selection of thioaptamers for diagnostics and therapeutics.
Thioaptamers offer advantages over normal phosphate ester backbone aptamers due to their enhanced affinity, specificity, and higher stability, largely due to the properties of the sulfur backbone modifications. Over the past several years, in vitro thioaptamer selection and bead-based thioaptamer selection techniques have been developed in our laboratory. Furthermore, several thioaptamers targeting specific proteins such as transcription factor NF-kappaB and AP-1 proteins have been identified. Selected thioaptamers have been shown diagnostic promise in proteome screens. Moreover, some promising thioaptamers have been shown in preliminary animal therapeutic dosing to increase survival in animal models of infection with West Nile virus.
Yang
Xianbin
X
Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology, University of Texas Medical Branch at Galveston, Texas 77555-1157, USA.
Wang
He
H
Beasley
David W C
DW
Volk
David E
DE
Zhao
Xu
X
Luxon
Bruce A
BA
Lomas
Lee O
LO
Herzog
Norbert K
NK
Aronson
Judith F
JF
Barrett
Alan D T
AD
Leary
James F
JF
Gorenstein
David G
DG
D000818:Animals; D052157:Aptamers, Nucleotide / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D051379:Mice; D040081:Protein Array Analysis; D040901:Proteomics; D013873:Thionucleotides / Q000627:therapeutic use; D014157:Transcription Factors / Q000037:antagonists & inhibitors; D014901:West Nile Fever / Q000188:drug therapy / Q000401:mortality
D052157:Aptamers, Nucleotide; D013873:Thionucleotides; D014157:Transcription Factors