title: [Investigation on vibrio cholera carried in aquatic products of littoral areas, Zhejiang Province].
abstract: OBJECTIVE
  To examine vibrio cholera (V.C) in aquatic products of littoral area, Zhejiang Province and to provide scientific evidence for administration of aquatic products and cholera epidemic control.
  
  
  METHODS
  All 990 samples of aquatic products collected from local markets, eateries and aquafarms in three chosen areas. Samples were proliferated in alkaline liquid medium, and purified in NO: 4 medium, the isolations were identified biochemically, and phenotype of strains were defined by phagocyte and coagulation with V.C. diagnostic serum. Three virulence genes (ctx, ace, zct) of the isolated strains were detected by polymerase chain reaction (PCR).
  
  
  RESULTS
  There were 1.41% samples caught by V.C., having a carrying rate highest in turtles of 8.9%. 14 strains were defined as three serogroups, and the numbers of Inaba, Ogawa, and Hikojima types were 2, 2, 10 respectively. Virulence genes had detected in 9 of 12 stains. All genes were detected in 5 strains, only ZOT genes in 3 strains, and both CTX and ACE genes in 1 strain.
  
  
  CONCLUSIONS
  Aquatic products from inshore in Zhejiang Province caught with V.C. strains might be divided into three serogroups. Most of them should be virulence genes. Cholera epidemic outbreak might be caused by those contaminated products.
authors.affiliation: Zhejiang Provincial Center for Disease Prevention and Control, Hangzhou 310009, China.
authors.forename: Hua-kun
authors.initials: HK
authors.lastname: Lü
authors.forename: En-fu
authors.initials: EF
authors.lastname: Chen
authors.forename: Shu-yun
authors.initials: SY
authors.lastname: Xie
authors.forename: Cheng-liang
authors.initials: CL
authors.lastname: Chai
authors.forename: Yu-dong
authors.initials: YD
authors.lastname: Wei
authors.forename: Shun-tang
authors.initials: ST
authors.lastname: Mo
authors.forename: Ju-lian
authors.initials: JL
authors.lastname: Ye
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
mesh_terms: D002681:China; D005516:Food Microbiology; D005798:Genes, Bacterial; D017747:Seafood / Q000382:microbiology*; D014734:Vibrio cholerae / Q000235:genetics / Q000302:isolation & purification*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors