title: Study of hemorheological parameters following partial hepatectomy in rats with chronic aluminium intoxication.
abstract: The aim of our work was to analyze the hemorheological parameters following partial hepatectomy in rats with chronic Al-intoxication (Al). Male Wistar rats were randomly assigned into four experimental groups (n=6 each one): Sham (rats subjected to simulated surgery); Al+Sham; Partial Hepatectomy (animals subjected to 65% liver resection) and Al+Partial Hepatectomy. Our results show that both Partial Hepatectomy and Al treatment produce a decrease of plasma cholesterol level, which showed a negative association with Rigidity Index increase (r(s)=-0.6475, p<0.05). The increase of Rigidity Index observed in Partial Hepatectomy, Al+Sham and Al+Partial Hepatectomy could be related to the increase of the proportion of non-discocytic erythrocytes, particularly stomatocytes, which determines a diminution of the Morphological Index. In the Altreated groups, greater changes in Rigidity Index and Morphological Index were observed. The relative viscosity of blood at a standard haematocrit of 40% was increased in Partial Hepatectomy, Al+Sham and Al+Partial Hepatectomy as compared to Sham, due to erythrocyte rigidity. On the other hand, we observed that the increase of plasma fibrinogen concentration correlates with augmentation of plasma viscosity (r(s)=0.689, p=0.004) for all the experimental groups studied. The results indicate that both administration of Al and Partial Hepatectomy induce microcytic hypocromic anaemia in the rats reflected by a significant decrease of haematocrit, mean corpuscular volume and mean corpuscular haemoglobin concentration. From these results, we conclude that in partially hepatectomized, Al-overloaded rats the decrease in erythrocyte deformability may be an important factor leading to the installation of anaemia.
authors.affiliation: Cátedra de Fisiología Humana, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Argentina.
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Contini
authors.forename: Stella
authors.initials: S
authors.lastname: Mahieu
authors.forename: Graciela
authors.initials: G
authors.lastname: Bazzoni
authors.forename: Claudio A
authors.initials: CA
authors.lastname: Bernal
authors.forename: Cristina E
authors.initials: CE
authors.lastname: Carnovale
mesh_terms: D000535:Aluminum / Q000097:blood / Q000633:toxicity*; D000740:Anemia / Q000209:etiology*; D000818:Animals; D001809:Blood Viscosity / Q000187:drug effects / Q000502:physiology*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D004195:Disease Models, Animal; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004909:Erythrocyte Indices; D004913:Erythrocytes, Abnormal / Q000473:pathology; D005340:Fibrinogen / Q000737:chemistry; D018056:Hemorheology / Q000187:drug effects*; D006498:Hepatectomy / Q000009:adverse effects*; D008297:Male; D011897:Random Allocation; D051381:Rats
chemical_list: D005340:Fibrinogen; D002784:Cholesterol; D000535:Aluminum