title: [Effect of several physiochemical factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension culture].
abstract: OBJECTIVE
  To illustrate the effects of several physiochemical factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension cultures.
  
  
  METHODS
  High performance liquid chromatography and plant tissue culture were applied.
  
  
  CONCLUSIONS
  Cell growth and isoflavone accumulation were significantly stimulated in P. lobata cell suspension cultures by the increase of the sucrose concentration. Maintaining the pH value at the range over 5. 4 to 5. 8 was most suitable for isoflavone accumulation in P. lobata cell suspension cultures. Cell dried weight and isoflavone accumulation decreased sharply with the increase of the treated concentration of active carbon, while XAD-4 significantly stimulated cell growth and isoflavone accumulation.
authors.affiliation: Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Agriculture, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Cong-Bing
authors.initials: CB
authors.lastname: Fang
authors.forename: He-Qin
authors.initials: HQ
authors.lastname: Li
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Wan
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Jiang
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002606:Charcoal / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000378:metabolism*; D010946:Plants, Medicinal / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D011137:Polystyrenes; D011145:Polyvinyls; D029908:Pueraria / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D013395:Sucrose / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D007529:Isoflavones; D011137:Polystyrenes; D011145:Polyvinyls; D002606:Charcoal; C012101:XAD-4 resin; D013395:Sucrose