Analyses of PCR products using DNA templates containing a consecutive deoxyinosine sequence.
We determined nucleotide incorporation specificity of four thermostable DNA polymerases opposite deoxyinosine in order to evaluate the mutagenic effect of the presence of a consecutive deoxyinosine sequence during PCR amplification. Deoxycytosine was the only incorporated nucleotide opposite deoxyinosine during PCR amplification, irrespective of origin of DNA polymerase.
Kobayashi
Atsushi
A
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Kitaoka
Motomitsu
M
Hayashi
Kiyoshi
K
D020029:Base Pairing; D001483:Base Sequence; D002384:Catalysis; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004795:Enzyme Stability; D007288:Inosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D016133:Polymerase Chain Reaction; D019711:Pyrococcus / Q000201:enzymology; D019914:Taq Polymerase / Q000378:metabolism; D013698:Templates, Genetic; D019712:Thermococcus / Q000201:enzymology; D013824:Thermus / Q000201:enzymology
D009711:Nucleotides; D007288:Inosine; D004247:DNA; D019914:Taq Polymerase; C012271:deoxyinosine