Identification of aberrant 2'-5' RNA linkage isomers by pellicular anion exchange chromatography.
During chemical RNA synthesis, many undesired products may be formed. In addition to the "n-x" sequences, depurination products, and incompletely deprotected oligonucleotides, linkage isomers may form during condensation and/or deprotection of the synthetic products. Under acidic conditions, bond migration may alter normal 3'-5' diesters to aberrant 2'-5' diesters. This results in isomers that are difficult to identify by MS and LC-MS techniques because the isomers have identical masses. HPLC methods for identification of these isomers have not advanced because the isomers are not expected to exhibit differences in hydrophobicity that allow resolution by reversed-phase columns. Neither are changes in ionic interactions anticipated for these isomers that would allow resolution by ion exchange methods. We observed that chromatography on pellicular anion exchange phases, but not on porous anion exchange phases, completely resolves oligonucleotides with very slight conformation differences (e.g., DNA vs. RNA of identical sequence). Because incorporation of 2'-5' linkages in RNA will alter solution conformation slightly, we considered that this pellicular ion exchanger might also allow resolution of identical RNA sequences harboring aberrant 2'-5' linkages from those lacking aberrant 2'-5' linkages. Using the nonporous DNAPac PA200 column, we demonstrated a chromatographic procedure for resolving synthetic RNA with aberrant linkages from their normally linked counterparts. Under certain conditions, aberrant isomers are not completely resolved from those containing only normal linkages. Therefore, we also developed an independent linkage-confirming method using a 5'-3' exonuclease. This enzyme produces incomplete digestion products during digestion of synthetic RNA containing aberrant 2'-5' linkages, and these are readily resolved by DNAPac PA200 chromatography.
Thayer
James R
JR
Dionex Corporation, Sunnyvale, CA 94086, USA. jim.thayer@dionex.com
Rao
Srinivasa
S
Puri
Nitin
N
Burnett
Christopher A
CA
Young
Michelle
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004247:DNA / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D009693:Nucleic Acid Hybridization; D009843:Oligoribonucleotides / Q000737:chemistry* / Q000302:isolation & purification; D010727:Phosphoric Diester Hydrolases; D012313:RNA / Q000096:biosynthesis / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry
D009843:Oligoribonucleotides; D034741:RNA, Small Interfering; D012313:RNA; D004247:DNA; D010727:Phosphoric Diester Hydrolases