Quantitative determination of lobe specificity of mRNA expression of androgen-dependent genes in the rat prostate gland.
The rodent prostate has a complex structure, consisting of a ventral prostate (VP), lateral prostate (LP), dorsal prostate (DP) and anterior prostate (AP), and most studies so far have focused on the VP. Androgen-responsive prostatic secretory proteins, such as prostatein and kallikreins, are mainly produced in the VP, but others are abundant in the LP and DP, though little is known about differences of androgen regulation among the different lobes. Here, the mRNA expression levels of some representative androgen-responsive genes, including those encoding prostatic secreted proteins, were quantitatively determined in each of the prostatic lobes of intact rats and castrated rats treated with testosterone alone or plus flutamide. The results show that the transcriptional regulation of prostatic secretory proteins differs greatly among lobes, generally being more tightly regulated in the VP. A number of growth factor mRNAs were differentially expressed in separate lobes and were regulated by testosterone in a lobe-specific manner. Lobe-specific regulation by androgen was also found for other genes, including the DAD-1 and calreticulin genes. Thus, hormone-dependent transcriptional regulation of prostate genes differs among lobes, and there is also interlobar diversity of basal mRNA expression levels.
Suzuki
Tomoharu
T
Department of Xenobiotic Metabolism and Molecular Toxicology, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.
Fujimoto
Nariaki
N
Kitamura
Shigeyuki
S
Ohta
Shigeru
S
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001835:Body Weight; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes; D008297:Male; D008954:Models, Biological; D009919:Orchiectomy; D009929:Organ Size; D009928:Organ Specificity / Q000235:genetics; D011467:Prostate / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D011944:Receptors, Androgen / Q000378:metabolism; D013739:Testosterone / Q000097:blood / Q000494:pharmacology; D014018:Tissue Distribution
D000728:Androgens; D012333:RNA, Messenger; D011944:Receptors, Androgen; D013739:Testosterone