Simultaneous use of highly acidic acridine and rigid chiral linker for efficient site-selective RNA scission.
To the middle of oligonucleotide, 9-amino-2-methoxy-6-nitroacridine (pKa = 8.8) and 9-amino-6-chloro-2-methoxyacridine (pKa = 10.5) were tethered through three linkers, and the abilities of these conjugates for site-selective activation of RNA (inducing site-selective scission by Lu(III)) were compared. The RNA-activating ability was strongly dependent on both the acidity of acridine and the structure of linker. Combination of highly acidic acridine and rigid chiral linker leads to unprecedented efficient site-selective RNA activation.
Shi
Yun
Y
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8904, Japan.
Machida
Kenzo
K
Kuzuya
Akinori
A
Komiyama
Makoto
M
D000166:Acridines / Q000737:chemistry*; D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D008187:Lutetium / Q000737:chemistry; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D012313:RNA / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D012680:Sensitivity and Specificity
D000166:Acridines; D009692:Nucleic Acid Heteroduplexes; D008187:Lutetium; D012313:RNA; D004247:DNA