title: Numerical chromosome abnormalities in 8-cell embryos generated from gamma-irradiated male mice in the absence and presence of vitamin E.
abstract: OBJECTIVE
  To investigate the effects of gamma-rays on male NMRI mice, in the absence or presence of vitamin E, on abnormalities in chromosome number in 8-cell embryos generated after mating with non-irradiated female mice.
  
  
  METHODS
  The 8 - 11 week old male NMRI mice were irradiated whole body with 4 Gy of gamma-rays alone or in combination with 200 international units (IU)/kg vitamin E administered 1 h prior to irradiation. After 4 days, they were mated at weekly intervals with superovulated, non-irradiated female mice in successive 6 weekly periods. About 68 h post coitous (p.c.), 8-cell embryos were fixed on slides using standard methods in order to screen for abnormalities in chromosome number.
  
  
  RESULTS
  In control embryos, 8% of metaphases were aneuploid whereas in embryos generated from irradiated mice, the frequency of aneuploidy increased dramatically at all post irradiation sampling times (p < 0.001). Administration of vitamin E one hour before irradiation, significantly decreased chromosomal aberrations in all 6 groups (p < 0.05).
  
  
  CONCLUSIONS
  Data indicate that gamma-irradiation affects spermatogenesis and causes DNA alterations in sperm that may lead to chromosome abnormalities in subsequent embryos. Administration of vitamin E before irradiation effectively reduced the frequency of chromosomal abnormalities. The mechanism(s) by which vitamin E reduces genotoxic effects of radiation could be via radical scavenging or antioxidative effects.
authors.affiliation: Department of Medical Genetics, Tarbiat Modares University, Tehran, Iran. mozdarah@modares.ac.ir
authors.forename: Hossein
authors.initials: H
authors.lastname: Mozdarani
authors.forename: Mahdieh
authors.initials: M
authors.lastname: Salimi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002869:Chromosome Aberrations; D004622:Embryo, Mammalian / Q000528:radiation effects*; D005260:Female; D005720:Gamma Rays; D008297:Male; D051379:Mice; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013091:Spermatogenesis / Q000528:radiation effects*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D011837:Radiation-Protective Agents; D014810:Vitamin E