SNPs analysis by use of protected oligonucleotide probes.
Loss of large amounts of DNA probe occurred at the deprotection step of the in situ synthesis of DNA microarrays for analysis of SNPs or gene expression. In this study, we developed "Protected Oligonucleotide Probes" that can avoid elimination of probe DNAs and allow their highly efficient synthesis. The protected oligonucleotide probes containing dC(ac) and d[oxoA(ac)] instead of dC and dA proved to have similar affinity for ssDNA having the complementary sequence and base recognition ability compared with the corresponding unmodified DNA probes.
Ohkubo
Akihiro
A
Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Midoriku, Yokohama, 226-8501, Japan.
Kasuya
Rintarou
R
Sakamoto
Kazushi
K
Taguchi
Haruhiko
H
Seio
Kohji
K
Sekine
Mitsuo
M
D020029:Base Pairing; D004277:DNA, Single-Stranded / Q000737:chemistry; D003839:Deoxyadenosines / Q000737:chemistry*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide
C520183:4-N-acetyl-2'-deoxycytidine; D004277:DNA, Single-Stranded; D003839:Deoxyadenosines; C520184:N-acetyl-8-oxo-2'-deoxyadenosine; D015345:Oligonucleotide Probes; D003841:Deoxycytidine