Electricity from low-level H2 in still air--an ultimate test for an oxygen tolerant hydrogenase.
We demonstrate an extreme test of O(2) tolerance for a biological hydrogen-cycling catalyst: the generation of electricity from just 3% H(2) released into still, ambient air using an open fuel cell comprising an anode modified with the unusual hydrogenase from Ralstonia metallidurans CH34, that oxidizes trace H(2) in atmospheric O(2), connected via a film of electrolyte to a cathode modified with the fungal O(2) reductase, laccase.
Vincent
Kylie A
KA
Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, UK.
Cracknell
James A
JA
Clark
Jeremy R
JR
Ludwig
Marcus
M
Lenz
Oliver
O
Friedrich
Bärbel
B
Armstrong
Fraser A
FA
D000388:Air; D001674:Bioelectric Energy Sources; D004560:Electricity; D004563:Electrochemistry; D006859:Hydrogen / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006864:Hydrogenase / Q000737:chemistry*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D043367:Ralstonia / Q000201:enzymology; D017382:Reactive Oxygen Species / Q000737:chemistry
D017382:Reactive Oxygen Species; D006859:Hydrogen; D006861:Hydrogen Peroxide; D006864:Hydrogenase; D010100:Oxygen