Specific adeno-associated virus serotypes facilitate efficient gene transfer into human and non-human primate mesenchymal stromal cells.
Mesenchymal stromal cells (MSCs) show great promise for ex vivo gene and cell-mediated therapies. The immunophenotype and in vitro differentiation capacity of primary baboon MSCs was demonstrated to be near-identical to that observed in human MSCs. To optimize gene transfer efficiency, we compared the efficiency of serotypes 1, 2, 3, 4, 5, 6, and 8 of adeno-associated virus (AAV) vectors for their ability to mediate transduction of human and baboon MSCs. AAV serotype 2 vectors were the most efficient in transducing MSCs from humans and baboons. As a reference, human Ad293 cells were transduced with these seven AAV serotypes, and were found to have the highest transduction levels followed by baboon MSCs, and then human MSCs. The order of increasing transduction efficiency for the serotypes tested was similar for human and baboon MSCs, but was different for human Ad293 cells. The transduction efficiency of MSCs isolated from different individuals was comparable within the same species. We also demonstrated that baboon MSCs transduced with AAV serotype 2 vectors retain their potential to differentiate into adipocytes in vitro, and can incorporate into injured muscle tissue of NODSCID mice in vivo. We detected beta-galactosidase reporter gene expression in host muscle tissue for up to 9 weeks in this study, indicating engraftment of transduced baboon MSCs and sustained transgene expression in vivo.
Chng
Keefe
K
Gene and Stem Cell Therapy Program, Centenary Institute of Cancer Medicine and Cell Biology, University of Sydney, NSW, Australia.
Larsen
Stephen R
SR
Zhou
Shangzhen
S
Wright
J Fraser
JF
Martiniello-Wilks
Rosetta
R
Rasko
John E J
JE
D017667:Adipocytes / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation; D017690:Cell Transplantation; D000229:Dependovirus / Q000145:classification* / Q000235:genetics; D018014:Gene Transfer Techniques; D006801:Humans; D008648:Mesoderm / Q000166:cytology*; D010215:Papio; D012703:Serotyping; D013045:Species Specificity; D017154:Stromal Cells / Q000637:transplantation; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase