The quaternary structure of the amidase from Geobacillus pallidus RAPc8 is revealed by its crystal packing.
The amidase from Geobacillus pallidus RAPc8, a moderate thermophile, is a member of the nitrilase enzyme superfamily. It converts amides to the corresponding acids and ammonia and has application as an industrial catalyst. RAPc8 amidase has been cloned and functionally expressed in Escherichia coli and has been purified by heat treatment and a number of chromatographic steps. The enzyme was crystallized using the hanging-drop vapour-diffusion method. Crystals produced in the presence of 1.2 M sodium citrate, 400 mM NaCl, 100 mM sodium acetate pH 5.6 were selected for X-ray diffraction studies. A data set having acceptable statistics to 1.96 A resolution was collected under cryoconditions using an in-house X-ray source. The space group was determined to be primitive cubic P4(2)32, with unit-cell parameter a = 130.49 (+/-0.05) A. The structure was solved by molecular replacement using the backbone of the hypothetical protein PH0642 from Pyrococcus horikoshii (PDB code 1j31) with all non-identical side chains substituted with alanine as a probe. There is one subunit per asymmetric unit. The subunits are packed as trimers of dimers with D3 point-group symmetry around the threefold axis in such a way that the dimer interface seen in the homologues is preserved.
Agarkar
Vinod B
VB
Advanced Research Centre for Applied Microbiology, Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa.
Kimani
Serah W
SW
Cowan
Donald A
DA
Sayed
Muhammed F-R
MF
Sewell
B Trevor
BT
D000581:Amidohydrolases / Q000737:chemistry*; D000595:Amino Acid Sequence; D001406:Bacillaceae / Q000201:enzymology*; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020836:Protein Structure, Quaternary; D016415:Sequence Alignment
D000581:Amidohydrolases; C019665:amidase