[Pingyangmycin emulsion inducing apoptosis in infantile proliferating capillary hemangiomas].
OBJECTIVE
To insight whether PYM emulsion induces apoptosis more rapidly in proliferating capillary hemangiomas so as to find the optimal method for hemangiomas' treatment.


METHODS
Thirty volunteers of infantile proliferating hemangiomas were divided into control group (15 cases) and mediational group (15 cases). PYM was made into emulsion and smeared on the surfaces of the lesion in mediational group with 3 times every day as well as only matrix in control group. The specimens were resected on day 7, then made into pathological slices and electron microscope slices in order to observe the cells microcosmic structure changes and ultrastructure changes. Furthermore , the apoptotic index of two groups were detected by the molecular biology method (TUNEL test ).


RESULTS
The number of apoptotic cells were lower in control group (AI 9.693 +/- 4.948) but higher apparently in mediational group (AI 39.373 +/- 15.927). The difference between two groups was significant (t = 6.893, P < 0.01) .


CONCLUSIONS
PYM emulsion can effectively accelerate apoptosis in infantile proliferating capillary hemangiomas . The mechanism is supposed to related to the blockage of cell cycle and activation of apoptotic signal transduction pathway.
Xu
Wei-li
WL
Department of Pediatric Surgery, the 2nd Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Niu
Ai-guo
AG
Li
Suo-lin
SL
Li
Zhen-dong
ZD
D017209:Apoptosis / Q000187:drug effects*; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D018324:Hemangioma, Capillary / Q000473:pathology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male
D001761:Bleomycin; C025703:bleomycetin