title: Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear.
abstract: In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patientos ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.
authors.affiliation: Department of Parasitology, School of Medicine, Pusan National University, Busan, Korea.
authors.forename: Sun Joo
authors.initials: SJ
authors.lastname: Lee
authors.forename: Hae Jin
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Jong Soo
authors.initials: JS
authors.lastname: Lee
authors.forename: Ying Hua
authors.initials: YH
authors.lastname: Xuan
authors.forename: Hyun-Hee
authors.initials: HH
authors.lastname: Kong
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Chung
authors.forename: Mee-Sun
authors.initials: MS
authors.lastname: Ock
authors.forename: Hak Sun
authors.initials: HS
authors.lastname: Yu
mesh_terms: D000048:Acanthamoeba / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D015823:Acanthamoeba Keratitis / Q000188:drug therapy / Q000469:parasitology*; D000293:Adolescent; D000818:Animals; D000981:Antiprotozoal Agents / Q000008:administration & dosage; D001251:Astigmatism / Q000628:therapy; D001645:Biguanides / Q000008:administration & dosage; D002710:Chlorhexidine / Q000008:administration & dosage; D003261:Contact Lenses / Q000009:adverse effects*; D004272:DNA, Mitochondrial / Q000032:analysis; D016054:DNA, Protozoan / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004202:Disinfectants / Q000008:administration & dosage; D005260:Female; D006801:Humans; D017964:Itraconazole / Q000008:administration & dosage; D009216:Myopia / Q000628:therapy; D021261:Parasitic Sensitivity Tests; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000981:Antiprotozoal Agents; D001645:Biguanides; D004272:DNA, Mitochondrial; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D004202:Disinfectants; D012337:RNA, Ribosomal, 18S; D017964:Itraconazole; C031233:polihexanide; D002710:Chlorhexidine