title: Incorporation of side-chain groups of basic amino acids into oligonucleotides via the 2'-position of uridine.
abstract: Certain amino acids and short peptides are known to act as enhancers of ribozyme-mediated RNA cleavage at low concentration of magnesium ion. Thus, covalent conjugates of oligonucleotides and some amino acids may have a potential for development as sequence-specific artificial ribonucleases. Here we would like to report an incorporation of basic amino acids, lysine and histidine, into oligonucleotides at the 2'-position of a uridine residue. The approach involves the 2'-O-alkylation and attachment of the corresponding amino acid by the alpha-amino group through a urethane-type linker.
authors.affiliation: Chemistry Department, M. V. Lomonossov Moscow State University, 1 Leninskie Gory, Moscow 119992, Russia.
authors.forename: Eugeny M
authors.initials: EM
authors.lastname: Zubin
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Stetsenko
authors.forename: Anna V
authors.initials: AV
authors.lastname: Kachalova
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Gait
authors.forename: Tatiana S
authors.initials: TS
authors.lastname: Oretskaya
mesh_terms: D024361:Amino Acids, Basic / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D014529:Uridine / Q000737:chemistry*
chemical_list: D024361:Amino Acids, Basic; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D006639:Histidine; D008239:Lysine; D014529:Uridine