title: Site-specific binding of chelerythrine to single cytosine and thymine bulges in DNA hairpins.
abstract: Chelerythrine exhibits pronounced antitumor and apoptosis activities. In order to provide insights into the molecular mechanism of its pharmacological effects, binding selectivity of chelerythrine to a series of single-base bulged DNA hairpins has been assayed by using spectrofluorometric technique. The results indicated that chelerythrine binds specifically to C- and T-bulge sites in DNA hairpins, while sanguinarine exhibits extremely weak binding selectivity to any single-base bulged DNA hairpins, although it has very similar structure with chelerythrine.
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Bai
authors.forename: Zhong-Zhen
authors.initials: ZZ
authors.lastname: Zhao
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Jiang
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry*; D053119:Benzophenanthridines / Q000737:chemistry*; D001665:Binding Sites; D003596:Cytosine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D007546:Isoquinolines / Q000737:chemistry; D009690:Nucleic Acid Conformation; D013941:Thymine / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D053119:Benzophenanthridines; D007546:Isoquinolines; D003596:Cytosine; D004247:DNA; C005705:sanguinarine; C016299:chelerythrine; D013941:Thymine