Opa interacting protein 5 (OIP5) is a novel cancer-testis specific gene in gastric cancer.
BACKGROUND
Identification of novel cancer-specific antigens is important for the advancement of immunotherapy. Our aim was to identify cancer-specific genes in gastric cancer.


METHODS
Using cDNA microarray analysis, we detected genes overexpressed specifically in gastric cancer cells. The expression levels of selected genes, including OIP5, was confirmed by real time RT-PCR analysis in tumor/normal paired bulk samples of 58 clinical cases. The expression levels of selected genes in normal tissues were also determined with a human total RNA master panel. We also compared the expression status of OIP5 with that of the other known cancer-testis specific genes.


RESULTS
Twenty-two genes were determined to be upregulated in gastric cancer cells. Among these, three genes (CDC6, Exo1, and OIP5) were selected and confirmed to be upregulated in the tumor tissue compared to normal tissue. A human total RNA master panel demonstrated that OIP5, but not Exo1 or CDC6, showed high specificity in testis. Thus OIP5 may be considered a cancer-testis specific gene. In 58 clinical cases of gastric cancer examined, we found OIP5 gene expression in 27 cases (47%). Thirteen of these 27 cases showed no expression of the known cancer specific genes such as MAGE-1, MAGE-3 or NY-ESO-1.


CONCLUSIONS
Using a combination of LMD and microarray, we identified OIP5 as a cancer-testis specific gene. Further expression analysis in a set of clinical cases revealed that OIP5 may be a novel immunotherapy target for patients with gastric cancer.
Nakamura
Yoshito
Y
Department of Surgery and Molecular Oncology, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumibaru, Beppu, 874-0838, Japan.
Tanaka
Fumiaki
F
Nagahara
Hisashi
H
Ieta
Keisuke
K
Haraguchi
Naotsugu
N
Mimori
Koshi
K
Sasaki
Atsushi
A
Inoue
Hiroshi
H
Yanaga
Katsuhiko
K
Mori
Masaki
M
D000230:Adenocarcinoma / Q000235:genetics* / Q000276:immunology; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D005095:Exoribonucleases / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D042282:Microdissection; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000276:immunology; D013737:Testis / Q000276:immunology
D000951:Antigens, Neoplasm; D002352:Carrier Proteins; D005095:Exoribonucleases