Aureispira marina gen. nov., sp. nov., a gliding, arachidonic acid-containing bacterium isolated from the southern coastline of Thailand.
Three strains of gliding bacteria, 24(T), 62 and 71, isolated from a marine sponge and algae from the southern coastline of Thailand, were studied using a polyphasic approach to clarify their taxonomic positions. A phylogenetic analysis based on 16S rRNA gene sequences showed that the three isolates formed a distinct lineage within the family 'Saprospiraceae' of the phylum Bacteroidetes and were related to members of the genus Saprospira. The G+C contents of the isolates were in the range 38-39 mol%. The major respiratory quinone was MK-7. The predominant cellular fatty acids were 20 : 4omega6c (arachidonic acid), 16 : 0 and iso-17 : 0. On the basis of morphological, physiological and chemotaxonomic characteristics, together with DNA-DNA hybridization data and 16S rRNA gene sequences, the isolates represent a novel species of a novel genus, for which the name Aureispira marina gen. nov., sp. nov. is proposed. The type strain of Aureispira marina is 24(T) (=IAM 15389(T)=TISTR 1719(T)).
Hosoya
Shoichi
S
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0032, Japan.
Arunpairojana
Vullapa
V
Thailand Institute of Scientific and Technological Research (TISTR), 35 Moo3, Technopolis, Khlong 5, Khlong Luang, Pathum Thani 12120, Thailand.
Suwannachart
Chatrudee
C
Thailand Institute of Scientific and Technological Research (TISTR), 35 Moo3, Technopolis, Khlong 5, Khlong Luang, Pathum Thani 12120, Thailand.
Kanjana-Opas
Akkharawit
A
Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90112, Thailand.
Yokota
Akira
A
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0032, Japan.
D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis*; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D056890:Eukaryota / Q000382:microbiology*; D008386:Marine Biology; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D011161:Porifera / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013785:Thailand
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D016718:Arachidonic Acid