title: Assembling liposomes by means of an oligonucleotide tagged with a lipophilic unit.
abstract: We synthesized DNA-cholesterol conjugate molecules and introduced them into 1-palmitoyl-2-oleoyl-sn-3-phosphocholine (POPC) liposomes to create DNA-tagged liposomes. These liposomes aggregated selectively depending on the sequence of DNA tag and the resultant pattern of the aggregation was a wide-spreading network structure, which was observed under a phase contrast microscope. The structure was minutely analyzed by transmission electron microscopy (TEM) and confocal laser-scanning microscopy. As a result, a hierarchical structure of the amphiphilic molecules was constructed from the liposome to the network via clustered structures.
authors.affiliation: Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Maru
authors.forename: Ken-ichiroh
authors.initials: K
authors.lastname: Shohda
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sugawara
mesh_terms: D000268:Adhesiveness; D002784:Cholesterol / Q000138:chemical synthesis / Q000737:chemistry; D004247:DNA / Q000138:chemical synthesis; D008081:Liposomes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D008081:Liposomes; D009841:Oligonucleotides; D004247:DNA; D002784:Cholesterol