Rabbit oviductal glycoprotein 1 gene: genomic organization polymorphism analysis and mRNA expression.
The OVGP1 is an oviductal glycoprotein that has positive effects on fertilization and early embryo development. We have amplified and sequenced the rabbit OVGP1 gene, which spans 13 kb and it is formed by 11 exons and 10 introns. To find polymorphisms, a region encompassing the promoter to intron 1 has been sequenced in 22 rabbits of the H, V, R, and A Spanish lines. We have identified five SNPs and one triallelic microsatellite in the promoter region, and three SNPs and one dinucleotide INDEL in intron 1. The 10 polymorphic sites cosegregate forming two haplotypes. The allelic frequencies of the microsatellite have been analyzed in 98 rabbits belonging to the four lines and the three alleles were found in all the lines. The relative quantification of the OVGP1 mRNA in liver, kidneys, lungs, skeletal muscle, ovary, uterus, and oviduct reveals that the OVGP1 expression in the oviduct is 5,500 higher than in the uterus or ovary, whereas a low level of basal expression is detected in non-reproductive tissues. We have also compared the mRNA expression between samples of oviducts taken from non-mated rabbit and samples of oviducts at different stages of the early pregnancy, but no significant differences were found.
Merchán
Maribel
M
Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain. mariaisabel.merchan@uab.es
Peiró
Rosa
R
Santacreu
M Antonia
MA
Francino
Olga
O
Folch
Josep M
JM
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D005260:Female; D015870:Gene Expression; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D012697:Serine Endopeptidases; C074599:oviductin