Inhibitive effects of quercetin on rabbit tenon capsule fibroblasts proliferation.
OBJECTIVE
To study the inhibitive effects of quercetin (QU) on the fibroblasts proliferation of rabbit Tenon's capsule and its mechanism.


METHODS
Cultured fibroblasts were exposed to different concentrations of QU solution and investigated by microculture tetrazolium (MTT) assay. The effect of QU was observed on cells cycle using the flow cytometer.


RESULTS
QU can suppress the proliferation of rabbit Tenon's capsule fibroblasts in vitro and show a dose-time dependent tendency. Flow cytometer results showed 26.92% cell increase in G1 phase, 23.50% decrease in S phase and 3.42% decrease in G2 phase.


CONCLUSIONS
QU can suppress the proliferation of rabbit Tenon's capsule fibroblasts in vitro and show a dose-time dependent tendency. QU may effect all phase of cell cycle and inhibit cell proliferation by inhibiting G1 phase transitting to S phase and G2 phase.
Liu
Su
S
Department of Ophthalmology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
Chen
Lin
L
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003228:Conjunctiva / Q000166:cytology*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D007399:Interphase / Q000187:drug effects*; D011794:Quercetin / Q000008:administration & dosage*; D011817:Rabbits; D013997:Time Factors
D000975:Antioxidants; D011794:Quercetin