title: Analysis of the secondary structure of a ribozyme that requires His as a cofactor.
abstract: A novel ribozyme that requires L-histidine for expression of the function was revealed from an in vitro selection experiment. Here, we report the secondary structural analysis of the ribozyme. Several deletion and point mutants were designed based on the possible secondary structures predicted by a thermodynamic method with nearest neighbor parameters. As a result, formation of two important stems was essential for folding into the active structure.
authors.affiliation: Department of Chemistry, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Kawakami
authors.forename: Chisako
authors.initials: C
authors.lastname: Maiya
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugimoto
mesh_terms: D003067:Coenzymes / Q000378:metabolism*; D006639:Histidine / Q000031:analogs & derivatives / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D003067:Coenzymes; D016337:RNA, Catalytic; D006639:Histidine