title: Free radical scavenging, anti-glycation and tyrosinase inhibition properties of a polysaccharide fraction isolated from the rind from Punica granatum.
abstract: The present investigation deals with the isolation of a polysaccharide fraction from Pomegranate (PFP), which was found to inhibit 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-Azinobis[3-ethylbenzothiazoline-6-sulfonate] ABTS(+) radical activities by 69% and 88%, respectively with 4 microg/ml concentration. The activity of PFP for free radical scavenging was also evaluated by electron spin resonance (ESR) Spectrophotometer and DPPH dot blot test. Anti-glycation ability of PFP was tested using BSA, which inhibited the formation of advanced glycation end-products (AGEs) by 28% and also inhibited the formation of fructosamine in the BSA/Glucose system. The inhibition of mushroom tyrosinase by 43% at 10 microg/ml concentration of PFP strongly suggested its efficacy as a possible skin whitener.
authors.affiliation: Microbial Biotechnology and Downstream Processing Lab, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur-721 302, India.
authors.forename: S
authors.initials: S
authors.lastname: Rout
authors.forename: R
authors.initials: R
authors.lastname: Banerjee
mesh_terms: D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D006031:Glycosylation / Q000187:drug effects*; D029561:Lythraceae / Q000737:chemistry*; D010084:Oxidation-Reduction; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D010851:Picrates / Q000037:antagonists & inhibitors; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000037:antagonists & inhibitors; D013844:Thiazoles / Q000037:antagonists & inhibitors
chemical_list: D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010455:Peptides; D010851:Picrates; D011134:Polysaccharides; D013451:Sulfonic Acids; D013844:Thiazoles; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl