Anti-tumor effects of naked plasmid DNA using a hydrodynamics-based procedure.
High levels of foreign gene expression in mouse hepatocytes can be achieved by the rapid injection of a large volume of naked plasmid (pDNA) into animals via the tail vein, the so-called hydrodynamics-based procedure. In this study, we evaluated the efficacy of hydrodynamics-based tumor necrosis factor alpha (TNFalpha) transfer for tumor treatment, in which the naked pDNA encoding TNFalpha was administered into the tail vein following a intravenous injection of B16 melanoma cells. The mice treated with TNFalpha-expressing pDNA displayed a profound reduction in lung metastasis.
Tsuyama
Youichi
Y
Department of Life and Environmental Science, Faculty of Engineering, Chiba Institute of Technology, Japan.
Kitajima
Masayuki
M
Miyano-Kurosaki
Naoko
N
Takaku
Hiroshi
H
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004247:DNA / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018014:Gene Transfer Techniques; D007275:Injections, Intravenous; D008175:Lung Neoplasms / Q000235:genetics / Q000556:secondary; D008546:Melanoma, Experimental / Q000235:genetics; D051379:Mice; D009362:Neoplasm Metastasis; D010957:Plasmids / Q000494:pharmacology*; D019076:Transgenes; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D000970:Antineoplastic Agents; D014409:Tumor Necrosis Factor-alpha; D004247:DNA