Autologous biograft and mesenchymal stem cells in treatment of the diabetic foot.
OBJECTIVE
This study was performed to test a new technique for treatment of chronic non-healing wound (diabetic ulcer) using autologous biograft composed of autologous skin fibroblasts on biodegradable collagen membrane (Coladerm) in combination with autologous mesenchymal stem cells (MSC) derived from the patient's bone marrow.


METHODS
The bone marrow aspirate of the patient with diabetic foot was applied directly to the wound and injected into the edges of the wound, finally covered with prepared autologous biograft. The patient received two additional treatments with cultured MSC on day 7 and 17.


RESULTS
The wound showed a steady overall decrease in wound size and an increase in the vascularity of the dermis and in the dermal thickness of the wound bed after 29 days of combined treatment.


CONCLUSIONS
Closing and healing of the non-healing diabetic ulcer was achieved by using the given combined therapy.
Vojtassák
Ján
J
Institute of Medical Biology and Genetics, Faculty of Medicine, Comenius University, Bratislava, Slovakia. jan.vojtassak@fmed.uniba.sk
Danisovic
L'ubos
L
Kubes
Miroslav
M
Bakos
Dusan
D
Jarábek
L'ubomír
L
Ulicná
Marcela
M
Blasko
Milan
M
D000368:Aged; D002478:Cells, Cultured; D003131:Combined Modality Therapy; D017719:Diabetic Foot / Q000601:surgery / Q000628:therapy*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D016038:Skin Transplantation / Q000379:methods*; D014182:Transplantation, Autologous; D014945:Wound Healing