Ribonucleopeptides recognize the phosphotyrosine residue.
Tools for selective recognition and sensing of kinase-catalyzed phosphorylation of Tyr or Ser/Thr residues located on a protein surface are essential for understanding signal transduction cascades of living cell. A stable complex of RNA and peptide (ribonucleopeptide) provides a new approach to tailor a receptor for small molecules. In vitro selection of an RNA-derived pool of ribonucleopeptide afforded a ribonucleopeptide receptor specific for phosphotyrosine (pTyr). Moreover, the ribonucleopeptide receptors are conveniently converted to fluorescent sensors for pTyr.
Hasegawa
Tetsuya
T
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
Yoshikawa
Susumu
S
Morii
Takashi
T
D001665:Binding Sites; D020650:Combinatorial Chemistry Techniques; D005456:Fluorescent Dyes / Q000737:chemistry*; D015694:Gene Products, rev / Q000737:chemistry; D008024:Ligands; D010455:Peptides / Q000737:chemistry*; D019000:Phosphotyrosine / Q000032:analysis*; D012313:RNA / Q000737:chemistry*; D012261:Ribonucleoproteins / Q000737:chemistry
D005456:Fluorescent Dyes; D015694:Gene Products, rev; D008024:Ligands; D010455:Peptides; D012261:Ribonucleoproteins; D019000:Phosphotyrosine; D012313:RNA