title: Comparison of ovine herpesvirus 2 genomes isolated from domestic sheep (Ovis aries) and a clinically affected cow (Bos bovis).
abstract: The rhadinovirus Ovine herpesvirus 2 (OvHV-2) is the causative agent of sheep-associated malignant catarrhal fever. OvHV-2 primarily affects ruminants and has a worldwide distribution. In this study, a composite sequence of OvHV-2 genomic DNA isolated from nasal secretions of sheep experiencing virus-shedding episodes was determined and compared with the sequence of OvHV-2 DNA isolated from a lymphoblastoid cell line derived from a clinically affected cow. The study confirmed the OvHV-2 sequence information determined for the cell line-isolated DNA and showed no apparently significant changes in the OvHV-2 genome during passage through a clinically susceptible species with subsequent maintenance in vitro. Amino acid identity between the predicted open reading frames (ORFs) of the two genomes was 94-100%, except for ORF73, which had an identity of 83%. Polymorphism in ORF73 was due primarily to variability in the G/E-rich repetitive central region of the ORF.
authors.affiliation: Animal Disease Research Unit, USDA-Agricultural Research Service, Washington State University, PO Box 646630, Pullman, WA 99164-6630, USA.
authors.forename: Naomi S
authors.initials: NS
authors.lastname: Taus
authors.affiliation: Animal Disease Research Unit, USDA-Agricultural Research Service, Washington State University, PO Box 646630, Pullman, WA 99164-6630, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Herndon
authors.affiliation: Animal Disease Research Unit, USDA-Agricultural Research Service, Washington State University, PO Box 646630, Pullman, WA 99164-6630, USA.
authors.forename: Donald L
authors.initials: DL
authors.lastname: Traul
authors.affiliation: Division of Medical Microbiology, School of Infection and Host Defence, University of Liverpool, Liverpool, UK.
authors.forename: James P
authors.initials: JP
authors.lastname: Stewart
authors.affiliation: Institute of Virology, University of Zurich, Zurich, Switzerland.
authors.forename: Mathias
authors.initials: M
authors.lastname: Ackermann
authors.affiliation: Animal Disease Research Unit, USDA-Agricultural Research Service, Washington State University, PO Box 646630, Pullman, WA 99164-6630, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: Animal Disease Research Unit, USDA-Agricultural Research Service, Washington State University, PO Box 646630, Pullman, WA 99164-6630, USA.
authors.forename: Donald P
authors.initials: DP
authors.lastname: Knowles
authors.affiliation: US Sheep Experiment Station, USDA-Agricultural Research Service, Dubois, ID, USA.
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Lewis
authors.affiliation: Program in Vector-borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Brayton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis* / Q000097:blood; D006566:Herpesviridae Infections / Q000662:veterinary / Q000821:virology; D008969:Molecular Sequence Data; D009297:Nasal Mucosa / Q000821:virology*; D018144:Rhadinovirus / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*
chemical_list: D004279:DNA, Viral