title: Correlation of structure and activity of short hairpin RNA.
abstract: To explore the relationship between the structure of the precursor of short hairpin RNA (shRNA) and the activity of the shRNA-expression vector, 32 kinds of vectors, which expressed shRNAs with different flanking sequences, were tested. Addition of the poly(A) enhanced the activity of shRNA driven by U6 promoter. The activity of poly (A) tagged U6 promoter driven shRNA was lower than that of the CMV promoter driven constructs, in spite of the identical sequence of both transcripts. The result indicated that it is not only the structure but also the cascade of the transcripts that affects the RNAi activity.
authors.affiliation: Department of Bioorganic Chemistry, Center of Molecular and Macromolecular Studies, Polish Academy of Science, Sienkiewicza 112, 90-363 Lodz, Poland.
authors.forename: Slawomir
authors.initials: S
authors.lastname: Antoszczyk
authors.forename: Kazunari
authors.initials: K
authors.lastname: Taira
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kato
mesh_terms: D001483:Base Sequence; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D034622:RNA Interference; D012322:RNA Precursors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D022661:RNA, Untranslated / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D012322:RNA Precursors; D022661:RNA, Untranslated