title: Development of electrochemical detection for methylcytosine and its application.
abstract: We report electrochemical detection of methylcytosine. We developed a bipyridine ligand possessing an amino linker. This ligand was used for methylcytosine-selective osmium oxidation and subsequent redox labeling. The DNA labeled methylcytosine selectively with anthraquinone showed the current signal through hybridization with a DNA probe fixed on a gold surface.
authors.affiliation: Frontier Research System, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tanaka
authors.forename: Kazuki
authors.initials: K
authors.lastname: Tainaka
authors.forename: Taku
authors.initials: T
authors.lastname: Kamei
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D044503:5-Methylcytosine / Q000032:analysis*; D000880:Anthraquinones / Q000737:chemistry; D015374:Biosensing Techniques; D015342:DNA Probes; D004563:Electrochemistry; D009992:Osmium / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: D000880:Anthraquinones; D015342:DNA Probes; C522084:N-(4-aminobutyl)-5-(2-(4-methylpyridin-2-yl)pyridin-4-yl)pentanamide; D009992:Osmium; D015082:2,2'-Dipyridyl; D044503:5-Methylcytosine