title: Fabrication of Au-DNA-Au nanostructure with new-type DNA-Au conjugate.
abstract: Nano devices fabricated from DNA and nanoparticles found many important applications in both biotechnology and nanotechnology. Conventionally, DNA was linked to gold surfaces through a linker. In this study, DNAs were directly attached to gold surfaces through S-Au bonds by modifying 5'-end of DNA with a mercapto group. The pH of the reaction mixture played an important role in DNA-gold conjugate fabrication. The DNA-Au conjugates were successfully obtained in pure forms and, with the use of this new type conjugate, Au-DNA-Au nanostructure was prepared. The Au-DNA-Au nanostructure was characterized by AFM.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Fenggang
authors.initials: F
authors.lastname: Pan
authors.forename: Jun
authors.initials: J
authors.lastname: Sumaoka
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Hirano
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Okutsu
authors.forename: Osamu
authors.initials: O
authors.lastname: Takeuchi
authors.forename: Hidemi
authors.initials: H
authors.lastname: Shigekawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force
chemical_list: D015342:DNA Probes; D006046:Gold; D004247:DNA