title: Nondestructive micro-patterning of proteinous occlusion bodies in water by femtosecond laser-induced mechanical force.
abstract: Some insect virus produces proteinous occlusion bodies named polyhedra, on which it is possible to immobilize functional proteins, such as green fluorescent proteins, antibodies, and growth factors, with keeping their biological activity. In this work, several kinds of polyhedra were micro-patterned by applying a mechanical force induced by femtosecond laser irradiation of a water buffer. In the printing process, since the laser is not directly irradiated on polyhedra, damages of polyhedra due to dryness and photothermal and photochemical reactions will be suppressed. The ability of the pattering was demonstrated by micro-sized checkerboard pattern, on which polyhedra with and without occluding enhanced green fluorescent protein were sorted. Furthermore, it was indicated that polyhedra are available as a scaffold of animal cell. This patterning method will be a promising technique to realize a microdevice in which functions of cell are artistically activated.
authors.affiliation: Department of Applied Physics and Venture Business Lab., Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. hosokawa@ap.eng.osaka-u.ac.jp
authors.forename: Yoichiroh
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kaji
authors.forename: Chisa
authors.initials: C
authors.lastname: Shukunami
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hiraki
authors.forename: Eiji
authors.initials: E
authors.lastname: Kotani
authors.forename: Hajime
authors.initials: H
authors.lastname: Mori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Masuhara
mesh_terms: D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D002479:Inclusion Bodies / Q000737:chemistry*; D007834:Lasers; D008422:Materials Testing; D019563:Mechanics; D051379:Mice; D041681:NIH 3T3 Cells; D011506:Proteins / Q000737:chemistry*; D013314:Stress, Mechanical; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D011506:Proteins; D014867:Water