Mutational analysis of Chk1, Chk2, Apaf1 and Rb1 in human malignant melanoma cell lines.
Four tumour suppressor genes (Chk1, Chk2, Apaf1 and Rb1) in nine human malignant melanoma cell lines were screened for a loss of gene expression, point mutations and small deletions/insertions by cDNA-based DGGE/SCCP analysis. In two cell lines alterations of the investigated genes could be demonstrated. This result confirms our assumption of the participation of dysfunctional p53 inducer/effector genes in human melanoma aetiology. Furthermore, it points towards the probable principal role of diverse alternative p53-pathway disruption mechanisms in this highly therapy-resistant malignancy without affecting p53 itself. To our knowledge, this is the first CHK1/CHK2 mutation screening in human melanoma.
Papp
Thilo
T
Department of Biological Sciences, Institute of Cell Biology and Biosystems Technology, University of Rostock, Albert Einstein Strasse 3, 18051 Rostock, Germany. thilo.papp@biologie.uni-rostock.de
Niemetz
Annett
A
Dosdahl
Nils
N
Kumar
Krishan
K
Schiffmann
Dietmar
D
D053477:Apoptotic Protease-Activating Factor 1 / Q000235:genetics*; D001483:Base Sequence; D045744:Cell Line, Tumor; D000071877:Checkpoint Kinase 1; D064447:Checkpoint Kinase 2; D003062:Codon; D004252:DNA Mutational Analysis; D016147:Genes, Tumor Suppressor; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D016160:Retinoblastoma Protein / Q000235:genetics*
C506592:APAF1 protein, human; D053477:Apoptotic Protease-Activating Factor 1; D003062:Codon; D016160:Retinoblastoma Protein; D011494:Protein Kinases; D064447:Checkpoint Kinase 2; C000605867:CHEK1 protein, human; C578634:CHEK2 protein, human; D000071877:Checkpoint Kinase 1; D017346:Protein Serine-Threonine Kinases