Influence of long-term treatment with cytosine arabinoside on telomere length in human HL60 cells.
Immortal tumor cells employ a telomere length maintenance mechanism to escape the normal limits on proliferation. We investigated whether treatment with cytosine arabinoside (AraC), whose triphosphate derivative AraCTP might partially inhibit the synthesis of C-rich telomere strands, is effective for inducing telomere shortening in human HL60 cells. Long-term treatment with 0.01 microM AraC was found to cause significant telomere lengthening, but had no marked effects on cell population doubling rates or morphology.
Yamaguchi
Toyofumi
T
Department of Biological Sciences, Teikyo University of Science and Technology, Uenohara, Yamanashi 409-0193, Japan.
Kazama
Kenta
K
Takashima
Jun
J
Ogawara
Tsukasa
T
Iyono
Saori
S
Inomata
Motoko
M
Saneyoshi
Mineo
M
D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D003561:Cytarabine / Q000008:administration & dosage / Q000494:pharmacology*; D015224:Dideoxynucleosides / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D016615:Telomere / Q000737:chemistry / Q000378:metabolism*
D000964:Antimetabolites, Antineoplastic; D015224:Dideoxynucleosides; D003561:Cytarabine; C051073:3'-azido-2',3'-dideoxyguanosine