title: The mechanically stable steam sterilization of bone grafts.
abstract: Bone allografts are the standard material used in augmentative bone surgery. However, steam-sterilized bone has a low mechanical stability and limited ossification based on low strain-adapted bone remodelling. Here we describe a new technique which allows the bone to be autoclaved without losing its mechanical stability and osteoconductivity. The compression strength of the new material was compared with steam-sterilized and fresh bone based on mechanical testing using bone cylinders (n=30/group). Allogeneic new material and fresh bone were press-fit implanted into rabbit patellar grooves and examined under fluorescent light and conventional microscopy. Initial healing was assessed after 30 d (n=5/group). Osseous integration and remodelling was studied after 100 d (n=12/group). Steam-sterilized bone showed no mechanical stability, whereas the new material was stiff and had compression curves similar to fresh bone; both groups showed equal degrees of direct ossification after 30 d, advanced bony ingrowth and remodelling after 100 d, and similar ingrowth depths on histomorphometric analysis. The new method preserved the stiffness and osteoconductivity of bone after steam sterilization, and microstructure, mineralization, and composition were conserved. This technique could be useful for bone banking in Third World countries.
authors.affiliation: Clinic for Maxillofacial Surgery, University of Munich, Lindwurmstr. 2a, 80336 Munich, Germany. draenert@yahoo.com
authors.forename: G F
authors.initials: GF
authors.lastname: Draenert
authors.forename: M
authors.initials: M
authors.lastname: Delius
mesh_terms: D000818:Animals; D016025:Bone Transplantation; D001842:Bone and Bones; D019245:Compressive Strength; D023421:Models, Animal; D011817:Rabbits; D011897:Random Allocation; D013227:Steam; D013242:Sterilization; D013552:Swine
chemical_list: D013227:Steam