Determination of selenium(IV) in pharmaceuticals and premixes by micellar liquid chromatography.
A simple micellar liquid chromatographic technique for the determination of selenium(IV) in pharmaceutical products (multi-vitamin tablets, syrups) and animal premixes after pre-column derivatization with 2,3-diaminonaphthalene was developed and validated. Hypersil ODS column, 10% (v/v) 1-butanol in 0.05 M sodium dodecyl sulfate as the mobile phase and UV detection at 378 nm and were used. The retention time was about 8 min. In the course of the validation study, the specificity of the method was demonstrated. Linearity was established in the range 0.33-3.3 microg ml(-1) of selenium(IV) content. The limits of detection and quantitation were 0.1 and 0.3 microg ml(-1), respectively. The method showed excellent accuracy (100.04%). Precision (repeatability) gave a relative standard deviation less than 1%. The influence of the various method parameters (robustness study) was also investigated.
Kulikov
Artem U
AU
Laboratory of Pharmacopoeial Analysis, Scientific and Expert Pharmacopoeial Centre, Astronomicheskaya street 33, 61085 Kharkov, Ukraine. kulikov@phukr.kharkov.ua
D000821:Animal Feed / Q000032:analysis*; D002214:Capsules; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D008823:Micelles; D004364:Pharmaceutical Preparations / Q000032:analysis*; D015203:Reproducibility of Results; D012643:Selenium / Q000032:analysis*; D012680:Sensitivity and Specificity; D013607:Tablets; D014815:Vitamins / Q000032:analysis
D002214:Capsules; D008823:Micelles; D004364:Pharmaceutical Preparations; D013607:Tablets; D014815:Vitamins; D012643:Selenium