Proteomic analysis of mouse growth plate cartilage.
Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS.
Belluoccio
Daniele
D
Murdoch Childrens Research Institute and the Department of Paediatrics, University of Melbourne, Royal Children's Hospital, Parkville, Victoria, Australia.
Wilson
Richard
R
Thornton
David J
DJ
Wallis
Tristan P
TP
Gorman
Jeffrey J
JJ
Bateman
John F
JF
D000818:Animals; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D016326:Extracellular Matrix Proteins / Q000302:isolation & purification / Q000378:metabolism; D006132:Growth Plate / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D051379:Mice; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
D016326:Extracellular Matrix Proteins; D020543:Proteome