title: Two-dimensional fractionation of complex mixture of oligonucleotides in microRNA size range.
abstract: Two-dimensional fractionation method suitable for analyzing oligonucleotide mixtures in the microRNA size range was devised. In this system, mobility of oligo-nucleotide is affected by its base composition in both dimensions. Thus, complex mixtures of oligonucleotides with similar chain length can be effectively fractionated.
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shiraishi
mesh_terms: D017360:Arabidopsis; D001483:Base Sequence; D005591:Chemical Fractionation / Q000379:methods*; D035683:MicroRNAs / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009841:Oligonucleotides / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D035683:MicroRNAs; D009841:Oligonucleotides