Complexation of thrombin-binding aptamer oligonucleotide carrying fluorescence resonance energy transfer (FRET) dyes at both termini with potassium ion.
Oligonucleotide derivatives, F-TBA-T and F-7TBA-T carrying thrombin-binding aptamer (TBA) sequence and FAM and TAMRA dyes at their each end were designed to achieve the fluorometric monitoring of potassium ion (K+) concentrations in a living organisms. These oligonucleotides could form a tetraplex structure upon addition of K+. Spectrophotometric experiments revealed that the fluorescence enhancement on the fluorescence resonance energy transfer (FRET) was larger in the case of F-7TBA-T having 7-nucleotides at 5'-end of TBA sequence than in the case of F-TBA-T due to the elimination of the quenching by dye-dye interaction.
Nagatoishi
Satoru
S
Department of Applied Chemistry, Kyushu University, Fukuoka, Japan.
Nojima
Takahiko
T
Takenaka
Shigeori
S
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002412:Cations / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011188:Potassium / Q000032:analysis* / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry
C106141:6-carboxytetramethylrhodamine; D052157:Aptamers, Nucleotide; D002412:Cations; D005452:Fluoresceins; D005456:Fluorescent Dyes; D012235:Rhodamines; C081676:thrombin aptamer; C024098:6-carboxyfluorescein; D011188:Potassium