Study of hemorheological parameters following partial hepatectomy in rats with chronic aluminium intoxication.
The aim of our work was to analyze the hemorheological parameters following partial hepatectomy in rats with chronic Al-intoxication (Al). Male Wistar rats were randomly assigned into four experimental groups (n=6 each one): Sham (rats subjected to simulated surgery); Al+Sham; Partial Hepatectomy (animals subjected to 65% liver resection) and Al+Partial Hepatectomy. Our results show that both Partial Hepatectomy and Al treatment produce a decrease of plasma cholesterol level, which showed a negative association with Rigidity Index increase (r(s)=-0.6475, p<0.05). The increase of Rigidity Index observed in Partial Hepatectomy, Al+Sham and Al+Partial Hepatectomy could be related to the increase of the proportion of non-discocytic erythrocytes, particularly stomatocytes, which determines a diminution of the Morphological Index. In the Altreated groups, greater changes in Rigidity Index and Morphological Index were observed. The relative viscosity of blood at a standard haematocrit of 40% was increased in Partial Hepatectomy, Al+Sham and Al+Partial Hepatectomy as compared to Sham, due to erythrocyte rigidity. On the other hand, we observed that the increase of plasma fibrinogen concentration correlates with augmentation of plasma viscosity (r(s)=0.689, p=0.004) for all the experimental groups studied. The results indicate that both administration of Al and Partial Hepatectomy induce microcytic hypocromic anaemia in the rats reflected by a significant decrease of haematocrit, mean corpuscular volume and mean corpuscular haemoglobin concentration. From these results, we conclude that in partially hepatectomized, Al-overloaded rats the decrease in erythrocyte deformability may be an important factor leading to the installation of anaemia.
Contini
María del Carmen
Mdel C
Cátedra de Fisiología Humana, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Argentina.
Mahieu
Stella
S
Bazzoni
Graciela
G
Bernal
Claudio A
CA
Carnovale
Cristina E
CE
D000535:Aluminum / Q000097:blood / Q000633:toxicity*; D000740:Anemia / Q000209:etiology*; D000818:Animals; D001809:Blood Viscosity / Q000187:drug effects / Q000502:physiology*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D004195:Disease Models, Animal; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004909:Erythrocyte Indices; D004913:Erythrocytes, Abnormal / Q000473:pathology; D005340:Fibrinogen / Q000737:chemistry; D018056:Hemorheology / Q000187:drug effects*; D006498:Hepatectomy / Q000009:adverse effects*; D008297:Male; D011897:Random Allocation; D051381:Rats
D005340:Fibrinogen; D002784:Cholesterol; D000535:Aluminum