Synthesis and properties of acridone-labeled base-discriminating fluorescent (BDF) nucleosides.
We have developed novel acridone-labelled BDF probe which showed its potential in recognizing opposite matched base from its target sequence via enhancement of fiuorescence intensity. This probe emit at a longer wavelength than previously reported pyrene-labelled BDF probe and thus can be used in DNA chip.
Saito
Yoshio
Y
NEWCAT Institue, School of Engineering, Nihon University and SORST, Japan Science and Tecnology Agency, Tamura, Koriyama 963-8642, Japan.
Hanawa
Kazuo
K
Bag
Subhendu Sekhar
SS
Motegi
Kaori
K
Saito
Isao
I
D000166:Acridines / Q000737:chemistry*; D054831:Acridones; D020137:Base Pair Mismatch; D005456:Fluorescent Dyes / Q000737:chemistry*; D009705:Nucleosides / Q000138:chemical synthesis / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D000166:Acridines; D054831:Acridones; D005456:Fluorescent Dyes; D009705:Nucleosides; D015345:Oligonucleotide Probes; C041300:acridone