Pyrene-labeled deoxyuridine and deoxyadenosine: fluorescent discriminating phenomena in their duplex and hairpin oligonucleotides.
Pyrene-labeled deoxyuracil and deoxyadenine units are useful unnatural nucleobases. These fluorescent nucleobase analogues allow strong interstrand stacking interactions to compensate for a loss of hydrogen bonding and exhibit a range of different emission intensities when they form duplexes with one another. These findings may provide new insights into the design of new probes and nucleobase analogues for applications in molecular biology. For this purpose, we have prepared an hairpin molecular beacon (MB) that incorporates an excimer unit in its closed state, and have utilized lambda(max) changing to discriminate between match and mismatch. This hairpin configuration is attractive because the synthesis of such an MB is relatively simple and inexpensive because it does not require two distinct processes to prepare the fluorophore and quencher.
Seo
Young Jun
YJ
National Research Laboratory, Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
Hwang
Gil Tae
GT
Kim
Byeang Hyean
BH
D003839:Deoxyadenosines / Q000737:chemistry*; D003857:Deoxyuridine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D003839:Deoxyadenosines; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011721:Pyrenes; D003857:Deoxyuridine