title: Gene expression responses of European flounder (Platichthys flesus) to 17-beta estradiol.
abstract: Male European flounder (Platichthys flesus) were intraperitoneally injected with 10mg/kg 17-beta estradiol and tissues taken from individuals over a timecourse of 16 days. The GENIPOL P. flesus cDNA microarray was employed to detect hepatic gene expression differences between fish treated with estradiol and saline controls. Known biomarkers of estrogen exposure, choriogenin L and vitellogenins, showed sustained induction over the time-course. Among 175 identified clones showing sustained statistically significant induction or repression, those associated with the Gene Ontology terms mitochondria, amino acid synthesis, ubiquitination and apoptosis were included amongst those induced while those associated with immune function, electron transport, cell signalling and protein phosphorylation were repressed. Thus, we show the gene expression response of an environmentally relevant fish species to a high dose of an estrogenic endocrine disruptor and also report the sequencing of a further 2121 flounder ESTs.
authors.affiliation: School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. t.d.williams@bham.ac.uk
authors.forename: Tim D
authors.initials: TD
authors.lastname: Williams
authors.forename: Amer M
authors.initials: AM
authors.lastname: Diab
authors.forename: Stephen G
authors.initials: SG
authors.lastname: George
authors.forename: Victoria
authors.initials: V
authors.lastname: Sabine
authors.forename: James K
authors.initials: JK
authors.lastname: Chipman
mesh_terms: D000818:Animals; D004958:Estradiol / Q000633:toxicity*; D005432:Flounder / Q000235:genetics*; D020869:Gene Expression Profiling; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D017422:Sequence Analysis, DNA; D014819:Vitellogenins / Q000235:genetics
chemical_list: D014819:Vitellogenins; D004958:Estradiol