title: Mutational analysis of Chk1, Chk2, Apaf1 and Rb1 in human malignant melanoma cell lines.
abstract: Four tumour suppressor genes (Chk1, Chk2, Apaf1 and Rb1) in nine human malignant melanoma cell lines were screened for a loss of gene expression, point mutations and small deletions/insertions by cDNA-based DGGE/SCCP analysis. In two cell lines alterations of the investigated genes could be demonstrated. This result confirms our assumption of the participation of dysfunctional p53 inducer/effector genes in human melanoma aetiology. Furthermore, it points towards the probable principal role of diverse alternative p53-pathway disruption mechanisms in this highly therapy-resistant malignancy without affecting p53 itself. To our knowledge, this is the first CHK1/CHK2 mutation screening in human melanoma.
authors.affiliation: Department of Biological Sciences, Institute of Cell Biology and Biosystems Technology, University of Rostock, Albert Einstein Strasse 3, 18051 Rostock, Germany. thilo.papp@biologie.uni-rostock.de
authors.forename: Thilo
authors.initials: T
authors.lastname: Papp
authors.forename: Annett
authors.initials: A
authors.lastname: Niemetz
authors.forename: Nils
authors.initials: N
authors.lastname: Dosdahl
authors.forename: Krishan
authors.initials: K
authors.lastname: Kumar
authors.forename: Dietmar
authors.initials: D
authors.lastname: Schiffmann
mesh_terms: D053477:Apoptotic Protease-Activating Factor 1 / Q000235:genetics*; D001483:Base Sequence; D045744:Cell Line, Tumor; D000071877:Checkpoint Kinase 1; D064447:Checkpoint Kinase 2; D003062:Codon; D004252:DNA Mutational Analysis; D016147:Genes, Tumor Suppressor; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D016160:Retinoblastoma Protein / Q000235:genetics*
chemical_list: C506592:APAF1 protein, human; D053477:Apoptotic Protease-Activating Factor 1; D003062:Codon; D016160:Retinoblastoma Protein; D011494:Protein Kinases; D064447:Checkpoint Kinase 2; C000605867:CHEK1 protein, human; C578634:CHEK2 protein, human; D000071877:Checkpoint Kinase 1; D017346:Protein Serine-Threonine Kinases