Expression of the Canavalia brasiliensis lectin (ConBr) in tobacco plants.
Tobacco plants were transformed with gene constructs encoding prepro-ConBr (Canavalia brasiliensis lectin). Transgenic plants confirmed by PCR expressed the recombinant protein as revealed by Western blot. However, the apparent molecular mass of the recombinant polypeptide (ca. 34 kDa) was higher than the native lectin (about 30 kDa), showing that further proteolytic processing of pro-ConBr was not detected.
da Silveira Carvalho
Cristina Paiva
CP
Laboratório de Genética Molecular, Depto. de Biologia, Bloco 906, Campus do Pici, Universidade Federal do Ceará, Av. Humberto Monte s/n, CEP 60.455-970, Fortaleza, Ceará, Brazil.
Correia
Tuana Oliveira
TO
Ribeiro
Sandra Mara Serafim
SM
Silva
Ingrid Cavalcante
IC
de Oliveira
José Tadeu Abreu
JT
Grangeiro
Thalles Barbosa
TB
D000818:Animals; D032449:Canavalia / Q000235:genetics*; D003001:Cloning, Molecular; D018515:Plant Leaves / Q000235:genetics; D037121:Plant Lectins / Q000096:biosynthesis* / Q000235:genetics*; D030821:Plants, Genetically Modified; D011817:Rabbits; D014026:Nicotiana / Q000235:genetics*
D037121:Plant Lectins; C039268:lectin, Canavalia