High selectivity detection of point mutation by DNA photochemical cross-linking.
We describe the investigation of the photochemical ligation based DNA chip for enhanced discrimination of single nucleotide polymorphisms (SNPs). Results show that the reaction takes place over a period of hours on the target, reaching more than 50% stage in 10 min. Significantly, a single mismatch in the target sequence yielded extremely little ligated product, with a measured rate that was 10(3)-fold lower than the complementary case.
Ogasawara
Shinzi
S
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
Fujimoto
Kenzo
K
D004247:DNA / Q000737:chemistry / Q000528:radiation effects; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010777:Photochemistry; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide
D004247:DNA