Agonist-induced endocytosis of rat somatostatin receptor 1.
Somatostatin-receptor 1 (sst1) is an autoreceptor in the central nervous system that regulates the release of somatostatin. Sst1 is present intracellularly and at the cell surface. To investigate sst1 trafficking, rat sst1 tagged with epitope was expressed in rat insulinoma cells 1046-38 (RIN-1046-38) and tracked by antibody labeling. Confocal microscopic analysis revealed colocalization of intracellularly localized rat sst1-human simplex virus (HSV) with Rab5a-green fluorescent protein and Rab11a-green fluorescent protein, indicating the distribution of the receptor in endocytotic and recycling organelles. Somatostatin-14 induced internalization of cell surface receptors and reduction of binding sites on the cell surface. It also stimulated recruitment of intracellular sst1-HSV to the plasma membrane. Confocal analysis of sst1-HSV revealed that the receptor was initially transported within superficial vesicles. Prolonged stimulation of the cells with the peptide agonist induced intracellular accumulation of somatostatin-14. Because the number of cell surface binding sites did not change during prolonged stimulation, somatostatin-14 was internalized through a dynamic process of continuous endocytosis, recycling, and recruitment of intracellularly present sst1-HSV. Accumulated somatostatin-14 bypassed degradation via the endosomal-lysosomal route and was instead rapidly released as intact and biologically active somatostatin-14. Our results show for the first time that sst1 mediates a dynamic process of endocytosis, recycling, and re-endocytosis of its cognate ligand.
Roosterman
Dirk
D
Department of Dermatology, and Ludwig Boltzmann Institute for Cell and Immunobiology of the Skin, Von-Esmarch-Strasse 58, D-48148 Münster, Germany. roosterman@gmx.net
Kreuzer
Oliver J
OJ
Brune
Nicole
N
Cottrell
Graeme S
GS
Bunnett
Nigel W
NW
Meyerhof
Wolfgang
W
Steinhoff
Martin
M
D000143:Acids / Q000494:pharmacology; D000818:Animals; D000954:Antigens, Surface / Q000187:drug effects / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D002478:Cells, Cultured; D004705:Endocytosis / Q000187:drug effects*; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D017481:Receptors, Somatostatin / Q000819:agonists* / Q000378:metabolism*; D013004:Somatostatin / Q000493:pharmacokinetics; D020691:rab GTP-Binding Proteins / Q000502:physiology; D000099193:rab11 GTP-Binding Proteins
D000143:Acids; D000954:Antigens, Surface; D017481:Receptors, Somatostatin; D013004:Somatostatin; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C101130:somatostatin receptor type 1