Estimation of DNA polymerase for improvement of rolling circle amplification.
Rolling Circle Amplification (RCA) is a technique to amplify the oligoncleotides containing tandemly repetitive sequence motif. Various repetitive sequences of which the motif unit is the circular template can be synthesized by extending the primer. Additionally, RCA need not change the temperature regulatory processes like PCR, but RCA has the gene amplification efficiency similar to PCR. Although, it contains the problem of the sequence confirmation and the activity of polymerase as the DNA polymerase I. We comparatively estimated of the various polymerases for the improvement of the RCA. Five DNA polymerases were estimated for RCA.
Yoshimura
Takeo
T
Department of Biological Functions and Engineering, Kyushu Institute of Technology, Wakamatsu, Kitakyushu, Fukuoka 808-0196, Japan.
Arikado
Saori
S
Ohuchi
Shokichi
S
D004247:DNA / Q000096:biosynthesis*; D004270:DNA, Circular / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques; D009841:Oligonucleotides / Q000737:chemistry; D020080:Tandem Repeat Sequences; D013698:Templates, Genetic
D004270:DNA, Circular; D009841:Oligonucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase