[The dynamics of oxidase activity during cultivation of basidiomycetes from the genus Trametes Fr].
Twenty strains of the wood-degrading fungi from the genus Trametes Fr., capable of synthesizing laccases, were screened according to the changes in the oxidase activity in a submerged culture. The range of maximal efficiency of various species with respect to extracellular oxidase activity was determined. The absence of correlation between the oxidase activity in a submerged culture and the size of colored zone on agar media (Bavendamm reaction) was demonstrated. The most efficient strains, T. hirsita 56 and T. ochracea 92-78, were used to produce laccases, homogeneous according to SDS electrophoresis data. A number of biochemical parameters characteristic of these enzymes were determined.
Gorshina
E S
ES
Rusinova
T V
TV
Biriukov
V V
VV
Morozova
O V
OV
Shleev
S V
SV
Iaropolov
A I
AI
D001487:Basidiomycota / Q000201:enzymology / Q000254:growth & development; D018929:Cell Culture Techniques; D005656:Fungal Proteins / Q000032:analysis* / Q000096:biosynthesis*; D042845:Laccase / Q000032:analysis* / Q000096:biosynthesis*; D010088:Oxidoreductases / Q000032:analysis / Q000096:biosynthesis; D020072:Polyporales / Q000201:enzymology* / Q000254:growth & development
D005656:Fungal Proteins; D010088:Oxidoreductases; D042845:Laccase