title: Embryonic stem cells using nuclear transfer.
abstract: Despite the fact that embryonic stem (ES) cells are able to differentiate into multiple therapeutically useful cell types, a prominent obstacle to their use is immune rejection by the recipient. One possible solution could be the derivation of ES cells from embryos generated by cloning using somatic cell nuclei from individual patients. The first section of this chapter describes progress in optimizing procedures for cloning, using the mouse as a model. The second section describes procedures for establishing ES cell lines from cloned mouse embryos.
authors.affiliation: Advanced Cell Technology, Biotech Five, Worcester, Massachusetts, USA.
authors.forename: Young
authors.initials: Y
authors.lastname: Chung
authors.forename: Sandy
authors.initials: S
authors.lastname: Becker
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation; D002875:Chromosomes / Q000648:ultrastructure; D003001:Cloning, Molecular / Q000379:methods; D003470:Culture Media; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D051379:Mice; D053652:Nuclear Transfer Techniques
chemical_list: D003470:Culture Media