title: Pancreatic autoantibodies in Italian patients with newly diagnosed type 1 diabetes mellitus over the age of 20 years.
abstract: The aim was to estimate the prevalence of the serological markers of pancreatic autoimmunity in a cohort of Italian patients with type 1 diabetes mellitus occurring after 20 years of age in order to determine the prevalence of autoimmune diabetes and the most sensitive autoantibody combination to be employed for the diagnosis. We investigated 57 patients (31 males and 26 females) at clinical diagnosis of type 1 diabetes. 35 patients were 21-40 years and 22 were 41-72 years of age. Autoantibodies to islet-cells (ICA) were detected by indirect immunofluorescence, while those against glutamic acid decarboxylase (GADA), tyrosine-phosphatase (IA2A) and insulin (IAA) were detected by radiobinding assays. A positive test for at least one of the pancreatic autoantibodies was found in 45 of the 57 patients (78.9%). Coupling two antibody tests, GADA and/or IAA were found in 73.7%, ICA and/or GADA in 71.9%, while GADA and/or IA2A were found in 70.2% of the patients. The most frequently positive test was for GADA (66.7%). In general, the frequency of diabetes-related antibodies was higher in the 21-40-year-old group compared to the 41-72-year-old group and in females than males. Based on the detection of pancreatic autoantibodies determination, the great majority of the adult patients with recent onset type 1 diabetes were found to be autoimmune in nature. The best cost/benefit combination is provided by coupling the detection of GADA and ICA.
authors.affiliation: Division of Endocrinology, Department of Medical and Surgical Sciences, University of Padua, Italy. corrado.betterle@unipd.it
authors.forename: C
authors.initials: C
authors.lastname: Betterle
authors.forename: F
authors.initials: F
authors.lastname: Lazzarotto
authors.forename: A
authors.initials: A
authors.lastname: Fusari
authors.forename: R
authors.initials: R
authors.lastname: Zanchetta
authors.forename: S
authors.initials: S
authors.lastname: Benedini
authors.forename: B
authors.initials: B
authors.lastname: Pedini
authors.forename: A
authors.initials: A
authors.lastname: Moscon
authors.forename: F
authors.initials: F
authors.lastname: Presotto
mesh_terms: D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D015551:Autoimmunity; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D005260:Female; D005968:Glutamate Decarboxylase / Q000276:immunology; D006801:Humans; D007330:Insulin Antibodies / Q000097:blood; D007515:Islets of Langerhans / Q000201:enzymology / Q000276:immunology*; D007558:Italy; D008297:Male; D008875:Middle Aged; D010179:Pancreas / Q000276:immunology*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D017027:Protein Tyrosine Phosphatases / Q000276:immunology
chemical_list: D001323:Autoantibodies; D007330:Insulin Antibodies; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D017027:Protein Tyrosine Phosphatases; D005968:Glutamate Decarboxylase