The stochastic nature of gene expression revealed at the single-molecule level.
Two recent papers have monitored the real-time synthesis of proteins in vivo at the single-molecule level. The work was done by two separate methods: fluorescent protein labeling and enzymatic amplification. Statistical analysis of the data reveals the inherent stochastic nature of gene expression.
Chen
Xin
X
Department of Chemistry, Texas A&M University, 3255 TAMU, College Station, Texas 77843, USA. cremer@mail.chem.tamu.edu
Cremer
Paul S
PS
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D013269:Stochastic Processes; D014158:Transcription, Genetic
D011506:Proteins; D049452:Green Fluorescent Proteins