Overexpression, purification, crystallization and preliminary X-ray analysis of uracil N-glycosylase from Mycobacterium tuberculosis in complex with a proteinaceous inhibitor.
Uracil N-glycosylase is an enzyme which initiates the pathway of uracil-excision repair of DNA. The enzyme from Mycobacterium tuberculosis was co-expressed with a proteinaceous inhibitor from Bacillus subtilis phage and was crystallized in monoclinic space group C2, with unit-cell parameters a = 201.14, b = 64.27, c = 203.68 A, beta = 109.7 degrees. X-ray data from the crystal have been collected for structure analysis.
Singh
Prem
P
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.
Talawar
Ramappa K
RK
Krishna
P D V
PD
Varshney
Umesh
U
Vijayan
M
M
D003460:Crystallization; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000737:chemistry; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D051981:Uracil-DNA Glycosidase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D014764:Viral Proteins / Q000737:chemistry*
D004791:Enzyme Inhibitors; D014764:Viral Proteins; C027619:uracil-DNA glycosylase inhibitor protein, B. subtilis bacteriophage; D051981:Uracil-DNA Glycosidase