title: Beneficial effects of autologous bone marrow mononuclear cell transplantation against elastase-induced emphysema in rabbits.
abstract: The authors investigated whether autologous bone marrow mononuclear cell (BMC) transplantation via the left and right main bronchi would mitigate elastase-induced pulmonary emphysema in rabbits. Four weeks after elastase administration, rabbits also receiving BMCs showed significantly better pulmonary function (FVC, FEV100, FEVPEF) and smaller alveolar airspaces, as indicated by a smaller mean linear intercept, than those receiving porcine pancreatic elastase (PPE) (200 U/kg) alone via the left and right main bronchi. BMCs also significantly reduced cell counts in bronchoalveolar lavage fluid, the incidence of apoptotic (TUNEL-positive) cells and matrix metalloproteinase (MMP)-2 expression, while increasing numbers of proliferative (Ki-67-positive) cells. Thus, BMCs may inhibit the progression to emphysema by attenuating inflammation, MMP-2 expression, and apoptosis, while enhancing alveolar cell proliferation.
authors.affiliation: Second Department of Internal Medicine, Regeneration and Advanced Medical Science, Graduate School of Medicine, Gifu University, Gifu, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Yuhgetsu
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ohno
authors.forename: Norihiko
authors.initials: N
authors.lastname: Funaguchi
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Asai
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sawada
authors.forename: Genzou
authors.initials: G
authors.lastname: Takemura
authors.forename: Shinya
authors.initials: S
authors.lastname: Minatoguchi
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Fujiwara
authors.forename: Takako
authors.initials: T
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D017209:Apoptosis; D016026:Bone Marrow Transplantation; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D004195:Disease Models, Animal; D004646:Emphysema / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D019084:Fluorescent Antibody Technique, Indirect; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009000:Monocytes / Q000637:transplantation*; D010196:Pancreatic Elastase / Q000009:adverse effects; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011817:Rabbits; D012129:Respiratory Function Tests; D014182:Transplantation, Autologous
chemical_list: D019394:Ki-67 Antigen; D010196:Pancreatic Elastase; D020778:Matrix Metalloproteinase 2