Synthesis of cross-linked circular DNAs using Hüisgen reaction.
Triazole-cross-linked ODNs were synthesized using Hüisgen reaction with 21 mer hairpin DNA possessing N-3 azidoethylthymidine and N-3 propargylthymidine at the 3' and 5'-terminals. Newly synthesized ODNs revealed thermally stable and their structures nearly retained those of non-cross-liked ODNs. This strategy is quite effective to prepare cross-linked circular ODNs.
Nakane
Masanori
M
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, kita-ku, Sapporo 060-0812, Japan.
Ichikawa
Satoshi
S
Matsuda
Akira
A
D003300:Copper / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D004270:DNA, Circular / Q000138:chemical synthesis* / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D013696:Temperature; D013936:Thymidine / Q000031:analogs & derivatives* / Q000737:chemistry; D014230:Triazoles / Q000737:chemistry
D003432:Cross-Linking Reagents; D004270:DNA, Circular; D009838:Oligodeoxyribonucleotides; D014230:Triazoles; D003300:Copper; D013936:Thymidine