Metabolism of 2-, 3- and 4-hydroxybenzoates by soil isolates Alcaligenes sp. strain PPH and Pseudomonas sp. strain PPD.
Pseudomonas sp. strain PPD and Alcaligenes sp. strain PPH isolated from soil by enrichment culture technique utilize 2-, 3- and 4-hydroxybenzoates as the sole source of carbon and energy. The degradation pathways were elucidated by performing whole-cell O(2) uptake, enzyme activity and induction studies. Depending on the mixture of carbon source and the preculture condition, strain PPH was found to degrade 2-hydroxybenzoate either via the catechol or gentisate route and has both salicylate 1-hydroxylase and salicylate 5-hydroxylase. Strain PPD utilizes 2-hydroxybenzoate via gentisate. Both strains degrade 3- and 4-hydroxybenzoate via gentisate and protocatechuate, respectively. Enzymes were induced by respective hydroxybenzoate. Growth pattern, O(2) uptake and enzyme activity profiles on the mixture of three hydroxybenzoates as a carbon source suggest coutilization by both strains. When 3- or 4-hydroxybenzoate grown culture was used as an inoculum, strain PPH failed to utilize 2-hydroxybenzoate via catechol, indicating the modulation of the metabolic pathways, thus generating metabolic diversity.
Deveryshetty
Jaigeeth
J
Biotechnology group, School of Biosciences and Bioengineering, Indian institute of Technology, Mumbai, India.
Suvekbala
V
V
Varadamshetty
Gautham
G
Phale
Prashant S
PS
D000421:Alcaligenes / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D003470:Culture Media; D049308:Dioxygenases / Q000378:metabolism; D004790:Enzyme Induction; D062385:Hydroxybenzoates / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D010226:Parabens / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D020156:Salicylic Acid / Q000378:metabolism; D012988:Soil Microbiology
D003470:Culture Media; D062385:Hydroxybenzoates; D010226:Parabens; C032948:3-hydroxybenzoic acid; D006899:Mixed Function Oxygenases; D049308:Dioxygenases; C038193:4-hydroxybenzoic acid; D020156:Salicylic Acid