Human embryo culture.
Human embryonic stem cells (hESCs) are derived from preimplantation embryos. Approximately 60% of human embryos are blocked during in vitro development. Although statistics are inconclusive, experience demonstrates that hESCs are more effectively derived from high-quality embryos. In this way, optimal human embryo culture conditions are a crucial aspect in any derivation laboratory. Embryos can be cultured solely with sequential media or cocultured on a monolayer of a given cell type. This chapter explores general aspects of human embryonic development, the concept of sequential culture and coculture, and specific protocols and procedures in which the authors are experienced, including the results obtained.
Mercader
Amparo
A
Instituo Valenciano de Infertilidad, Instituto Universitario, Vanencia, Spain.
Valbuena
Diana
D
Simón
Carlos
C
D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry* / Q000592:standards*; D046149:Embryo Culture Techniques / Q000379:methods* / Q000592:standards; D004622:Embryo, Mammalian / Q000166:cytology / Q000196:embryology*; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans
D003470:Culture Media