Ab initio DNA synthesis accelerated by endonuclease.
In the presence of an endonuclease that digests double-stranded DNA, DNA polymerase efficiently synthesizes and amplifies DNA from dNTPs in the absence of any added template and primer nucleic acid. This cut-polymerization DNA synthesis (Cut-grow) can be carried out under a wide range of temperature (4-85 degrees C) by many endonucleases and DNA polymerases. The high efficiency of Cut-grow results from an efficient exponential amplification involving digestion-elongation cycles. Our findings suggest that digestion of nucleic acids may play an important role during the evolution of genetic material for procreating the diversification of genetic information on the early earth.
Liang
Xingguo
X
Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
Li
Benjamin Chun-Yeung
BC
Jensen
Kari
K
Frank-Kamenetskii
Maxim D
MD
D004247:DNA / Q000096:biosynthesis* / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D019143:Evolution, Molecular; D008957:Models, Genetic; D020080:Tandem Repeat Sequences
D004247:DNA; D004259:DNA-Directed DNA Polymerase; D004706:Endodeoxyribonucleases; D015246:Deoxyribonuclease EcoRI