title: Study of growth parameters and potentialities of differentiation of multipotent mesenchymal stromal cells from rat bone marrow in vitro.
abstract: The dynamics of growth and proliferative activity of the population of multipotent mesenchymal stromal cells from rat bone marrow was studied during 7 passages. The efficiency of colony formation, the morphology of multipotent mesenchymal stromal cells, and the possibility of spontaneous and induced differentiation were studied. The rat bone marrow fibroblast-like multipotent mesenchymal stromal cells are capable of clonal growth; their proliferative activity and the yield remained high until passage 4, but then decreased. Induction of osteo- or adipogenic differentiation of bone marrow multipotent mesenchymal stromal cells increased the percentage of morphologically modified cells carrying specific markers.
authors.affiliation: Department of Prediction of Conservative Treatment Efficiency, P. A. Hertzen Oncological Institute, Russian Ministry of Health.
authors.forename: N S
authors.initials: NS
authors.lastname: Sergeeva
authors.forename: I K
authors.initials: IK
authors.lastname: Sviridova
authors.forename: V A
authors.initials: VA
authors.lastname: Kirsanova
authors.forename: S A
authors.initials: SA
authors.lastname: Akhmedova
authors.forename: N V
authors.initials: NV
authors.lastname: Marshutina
authors.forename: V S
authors.initials: VS
authors.lastname: Sergeeva
authors.forename: D Yu
authors.initials: DY
authors.lastname: Bednik
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000378:metabolism