Differential diagnosis between complete and partial mole using a TSSC3 antibody: correlation with DNA polymorphic marker analysis.
OBJECTIVE
To investigate the use of maternally expressed, imprinted genes for the differential diagnosis of complete hydatidiform mole (CHM) and partial hydatidiform mole (PHM).


METHODS
Expression patterns of imprinted genes in CHM were validat ed by microarray analysis. Twenty CHMs and 10 PHMs were then subjected to Western blot analysis and immunostaining with appropriate antibodies.


RESULTS
TSSC3 (also known as PHLDA2, IPL), SLC22A1L, KCNQ1 and decorin were shown to be down-regulated, with the suppression of TSSC3 most marked. In all 20 CHM cases for which the diagnosis had been confirmed by DNA polymorphic markers, the expression of TSSC3 was completely lost on Western blots. In contrast, in 10 PHMs that had also been diagnosed by DNA analysis, TSSC3 was expressed normally. Immunohistochemistry showed an identical result.


CONCLUSIONS
Complete silencing of TSSC3 expression in CHM offers a convenient and novel diagnostic strategy for diagnosing molar lesions.
Kato
Hidenori
H
Department of Gynecology, National Hokkaido Cancer Center, Sapporo City, Japan. hidekato@sap-cc.go.jp
Wake
Norio
N
D014408:Biomarkers, Tumor / Q000235:genetics; D002824:Chorionic Villi; D003937:Diagnosis, Differential; D015536:Down-Regulation; D005260:Female; D020868:Gene Silencing; D018392:Genomic Imprinting / Q000235:genetics*; D006801:Humans; D006828:Hydatidiform Mole / Q000175:diagnosis* / Q000235:genetics*; D007150:Immunohistochemistry; D009687:Nuclear Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy
D014408:Biomarkers, Tumor; D009687:Nuclear Proteins; D027701:Organic Cation Transport Proteins; C111653:SLC22A18 protein, human; C109277:TSSC3 protein