title: Regulation of DNA duplex formation by hypoxic irradiation: one-electron reduction characteristics of oligodeoxynucleotides possessing 2-oxoalkyl functional group.
abstract: We synthesized oligodeoxynucleotides (ODN) possessing 2-oxoalkyl group on thymidine (d(oxo)T) to characterize the radiolytic reduction in aqueous solution. Corresponding unmodified ODNs were generated from ODNs containing d(oxo)T upon hypoxic irradiation. Enzymatic digestion of the duplex consisting of the hypoxically irradiated ODN containing d(oxo)T and its complementary strand resulted in an efficient cleavage, but no cleavage was observed when a control duplex was digested without irradiation.
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Tanabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kanezaki
authors.forename: Nao
authors.initials: N
authors.lastname: Hirata
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Goto
authors.forename: Sei-ichi
authors.initials: S
authors.lastname: Nishimoto
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D004583:Electrons; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000528:radiation effects*; D010084:Oxidation-Reduction; D011839:Radiation, Ionizing; D013936:Thymidine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D013936:Thymidine