title: Diacyltrehalose of Mycobacterium tuberculosis inhibits lipopolysaccharide- and mycobacteria-induced proinflammatory cytokine production in human monocytic cells.
abstract: The lipids located in the outer layer of Mycobacterium tuberculosis, which include sulfolipid, phthiocerol dimycocerosate (PDIM), diacyltrehalose, and polyacyltrehalose, may play a role in host-pathogen interactions. These lipids were purified using thin-layer chromatography, and their ability to induce proinflammatory cytokines in human monocytes and in a human acute monocytic leukemia cell line (THP-1) was examined. None of the lipids tested induced significant interleukin (IL)-12p40 or tumor necrosis factor (TNF)-alpha production in monocytic cells. Diacyltrehalose significantly inhibited lipopolysaccharide- and M. tuberculosis-induced IL-12p40, TNF-alpha, and IL-6 productions in human monocytes, whereas other lipids had no effect. However, diacyltrehalose was unable to inhibit peptidoglycan-induced IL-12p40 production. These results suggest that diacyltrehalose is a mycobacterial factor capable of modulating host immune responses.
authors.affiliation: Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Korea.
authors.forename: Kil-Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Vinod S
authors.initials: VS
authors.lastname: Dubey
authors.forename: Pappachan E
authors.initials: PE
authors.lastname: Kolattukudy
authors.forename: Chang-Hwa
authors.initials: CH
authors.lastname: Song
authors.forename: A-Rum
authors.initials: AR
authors.lastname: Shin
authors.forename: Saet-Byel
authors.initials: SB
authors.lastname: Jung
authors.forename: Chul-Su
authors.initials: CS
authors.lastname: Yang
authors.forename: Su-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Eun-Kyeong
authors.initials: EK
authors.lastname: Jo
authors.forename: Jeong-Kyu
authors.initials: JK
authors.lastname: Park
authors.forename: Hwa-Jung
authors.initials: HJ
authors.lastname: Kim
mesh_terms: D045744:Cell Line, Tumor; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D053762:Interleukin-12 Subunit p40 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008055:Lipids / Q000276:immunology* / Q000302:isolation & purification; D009000:Monocytes / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000276:immunology*; D014199:Trehalose / Q000031:analogs & derivatives* / Q000276:immunology* / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D053762:Interleukin-12 Subunit p40; D015850:Interleukin-6; D008055:Lipids; D014409:Tumor Necrosis Factor-alpha; D014199:Trehalose