title: Conformational preference of polyglycine in solution to elongated structure.
abstract: Do polypeptide chains ever behave like a random coil? In this report we demonstrate that glycine, the residue with the fewest backbone restrictions, exhibits a strong preference for an extended conformation in solution when polymerized in short segments of polyglycine. A model peptide system comprised of two unique tripeptide units, between which 1 to 18 glycine residues are inserted, is characterized by NMR and by small-angle X-ray scattering (SAXS). The residual dipolar coupling (RDC) values of the two tripeptide units are insensitive to changes in number of intervening glycines, suggesting that extension of the linker does not alter the average angular relationship between the tripeptides. Polyglycine segments longer than nine residues form insoluble aggregates. SAXS measurements using synchrotron radiation provide direct evidence that polyglycine peptides adopt elongated conformations. In particular, the construct with a linker with six glycines showed a scattering profile indicative of a monomeric state with a radius of gyration and the maximum dimension of 9.1 A and approximately 34 A, respectively. The ensemble averaged global structure of this 12-mer peptide can best be approximated by a cylinder with a radius of 4 A and a length of approximately 33 A, making it intermediate in extension between a beta strand and an alpha helix.
authors.affiliation: Department of Biological Chemistry, The Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA. ohnishi@gsc.riken.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohnishi
authors.forename: Hironari
authors.initials: H
authors.lastname: Kamikubo
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Onitsuka
authors.forename: Mikio
authors.initials: M
authors.lastname: Kataoka
authors.forename: David
authors.initials: D
authors.lastname: Shortle
mesh_terms: D000595:Amino Acid Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D012996:Solutions
chemical_list: D003432:Cross-Linking Reagents; D010455:Peptides; D012996:Solutions; C011080:polyglycine