Assembling liposomes by means of an oligonucleotide tagged with a lipophilic unit.
We synthesized DNA-cholesterol conjugate molecules and introduced them into 1-palmitoyl-2-oleoyl-sn-3-phosphocholine (POPC) liposomes to create DNA-tagged liposomes. These liposomes aggregated selectively depending on the sequence of DNA tag and the resultant pattern of the aggregation was a wide-spreading network structure, which was observed under a phase contrast microscope. The structure was minutely analyzed by transmission electron microscopy (TEM) and confocal laser-scanning microscopy. As a result, a hierarchical structure of the amphiphilic molecules was constructed from the liposome to the network via clustered structures.
Maru
Naoto
N
Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Shohda
Ken-ichiroh
K
Sugawara
Tadashi
T
D000268:Adhesiveness; D002784:Cholesterol / Q000138:chemical synthesis / Q000737:chemistry; D004247:DNA / Q000138:chemical synthesis; D008081:Liposomes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*
D008081:Liposomes; D009841:Oligonucleotides; D004247:DNA; D002784:Cholesterol