title: Comparison of the insulin reaction of peripheral blood T cells between healthy Holstein dairy cows and JB during the periparturient period.
abstract: To compare the changes in the insulin reaction of Holstein dairy cows and Japanese Black cows (JB) during the periparturient period, the insulin resistance test in vivo and lymphocytes proliferation with insulin in vitro were performed. Ten healthy Holstein dairy cows (Holstein group) and 10 healthy JB cows (JB group) used in this study were observed on days 60, 40, and 20 before calving and days 7 and 20 after calving. In insulin resistance reaction in vivo and in vitro, a low insulin-stimulated glucose disposal rate and lymphocyte proliferation with insulin were observed in the Holstein group compared with the JB group during the experimental period. An analysis of the lymphocytes cultured with insulin showed that the percentage of CD4+CD45R- T cells in the Holstein group was significantly lower than that of the JB group before day 20. These findings indicate that T cells reaction to insulin in healthy periparturient Holstein cows is lower than that in Japanese Black.
authors.affiliation: School of Veterinary Medicine and Animal Sciences, Kitasato University, Aamori, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Madoka
authors.initials: M
authors.lastname: Kitagawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kohiruimaki
authors.forename: Erika
authors.initials: E
authors.lastname: Tanami
authors.forename: Machiko
authors.initials: M
authors.lastname: Masui
authors.forename: Tomohito
authors.initials: T
authors.lastname: Hayashi
authors.forename: Takaaki
authors.initials: T
authors.lastname: Ando
authors.forename: Daisaku
authors.initials: D
authors.lastname: Watanabe
authors.forename: Masateru
authors.initials: M
authors.lastname: Koiwa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sato
authors.forename: Seiichi
authors.initials: S
authors.lastname: Kawamura
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002417:Cattle; D049109:Cell Proliferation; D005260:Female; D007328:Insulin / Q000494:pharmacology*; D007333:Insulin Resistance / Q000502:physiology*; D036801:Parturition / Q000502:physiology*; D011247:Pregnancy; D013045:Species Specificity; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology*
chemical_list: D001786:Blood Glucose; D007328:Insulin