title: Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells.
abstract: The yellow fever virus attenuated 17D vaccine strain is a safe and effective vaccine and a valuable model system for evaluating immune responses against attenuated viral variants. This study compared the in vitro interactions of the commercially available yellow fever vaccine (YF-VAX), Dengue virus and the live-attenuated dengue vaccine PDK50 with dendritic cells (DCs), the main antigen-presenting cells at the initiation of immune responses. Similar to PDK50, infection with YF-VAX generated activated DCs; however, for YF-VAX, activation occurred with limited intracellular virus replication. The majority of internalized virus co-localized with endolysosomal markers within 90 min, suggesting that YF-VAX is processed rapidly in DCs. These results indicate that restricted virus replication and lysosomal compartmentalization may be important contributing factors to the success of the YF-VAX vaccine.
authors.affiliation: Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
authors.forename: Dupeh R
authors.initials: DR
authors.lastname: Palmer
authors.affiliation: Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
authors.forename: Stefan
authors.initials: S
authors.lastname: Fernandez
authors.affiliation: Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
authors.forename: John
authors.initials: J
authors.lastname: Bisbing
authors.affiliation: Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, MD, USA.
authors.forename: Kristina K
authors.initials: KK
authors.lastname: Peachman
authors.affiliation: Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, MD, USA.
authors.forename: Mangala
authors.initials: M
authors.lastname: Rao
authors.affiliation: Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
authors.forename: Dave
authors.initials: D
authors.lastname: Barvir
authors.affiliation: Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
authors.forename: Vicky
authors.initials: V
authors.lastname: Gunther
authors.affiliation: Infectious Disease Directorate, Naval Medical Research Institute, Silver Spring, MD, USA.
authors.forename: Timothy
authors.initials: T
authors.lastname: Burgess
authors.affiliation: Department of Microbiology and Immunology, Georgetown University School of Medicine, Washington DC, USA.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Kohno
authors.affiliation: Department of Microbiology and Immunology, Georgetown University School of Medicine, Washington DC, USA.
authors.forename: R
authors.initials: R
authors.lastname: Padmanabhan
authors.affiliation: Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
authors.forename: Wellington
authors.initials: W
authors.lastname: Sun
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003713:Dendritic Cells / Q000821:virology*; D006801:Humans; D008247:Lysosomes / Q000378:metabolism* / Q000821:virology; D014709:Vero Cells; D014779:Virus Replication; D022341:Yellow Fever Vaccine / Q000009:adverse effects / Q000276:immunology; D015005:Yellow fever virus / Q000276:immunology / Q000502:physiology*
chemical_list: D022341:Yellow Fever Vaccine