Role of the STK15 Phe31Ile polymorphism in renal cell carcinoma.
The search of inherited cancer susceptibility factors is an important subject in cancer epidemiology. Analyses of single nucleotide polymorphisms (SNP) in various genes revealed a correlation between the presence of specific allelic variants and cancer predisposition in diverse malignancies. STK15 is an important protein in control of the integrity of the mitotic spindle apparatus and genomic stability. We analysed the distribution of the functionally important T91A SNP in the STK15 gene in a cohort of renal cell carcinoma (RCC) patients and compared it to the distribution in a control group without malignancies. DNA from formalin-fixed, paraffin-embedded healthy renal tissue (RCC patients) or peripheral blood samples (control group) was isolated according to standard protocols. Allelic variant of STK15 nucleotide 91 was determined using restriction fragment length polymorphism (RFLP) analysis. Overall, 156 RCC patients and 158 patients without any malignancy were analysed. The distribution of the STK15 SNP in RCC patients (T/T, 58.97%; A/T, 36.53%; A/A, 4.49%) did not significantly differ from that of the control group (T/T, 51.27%; A/T, 41.14%; A/A, 7.59%). There was also no correlation between genotype and tumour grade or stage or other histopathological characteristics of the tumours. This first analysis of the STK15 T91A SNP in RCC patients revealed no correlation between a certain allelic variant and an increased risk for RCC.
Hammerschmied
Christine G
CG
Department of Urology, University of Regensburg, D-93042 Regensburg, Germany. christine.hammerschmied@klinik.uni-regensburg.de
Stoehr
Robert
R
Walter
Bernhard
B
Wieland
Wolf F
WF
Hartmann
Arndt
A
Blaszyk
Hagen
H
Denzinger
Stefan
S
D064096:Aurora Kinase A; D064127:Aurora Kinases; D002292:Carcinoma, Renal Cell / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005838:Genotype; D006801:Humans; D007532:Isoleucine / Q000235:genetics; D007680:Kidney Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D010649:Phenylalanine / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*
D007532:Isoleucine; D010649:Phenylalanine; C576917:AURKA protein, human; D064096:Aurora Kinase A; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases