Prototheca zopfii genotype 2: the causative agent of bovine protothecal mastitis?
In order to clarify the epidemiology of bovine protothecal mastitis, 30 Prototheca zopfii mastitis isolates were genetically investigated. Based on the 18S rDNA, which allows a differentiation of the former species P. zopfii in two distinct P. zopfii genotypes and Prototheca blaschkeae sp. nov., newly developed genotype-specific PCR-assays as well as RFLP-assays were applied. All mastitis isolates investigated could be assigned to P. zopfii genotype 2 suggesting that this genotype is the aetiological agent of bovine Prototheca mastitis.
Möller
Asja
A
Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierklinken 1, D-04103 Leipzig, Germany.
Truyen
Uwe
U
Roesler
Uwe
U
D000818:Animals; D002417:Cattle; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005838:Genotype; D005858:Germany / Q000453:epidemiology; D007239:Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008414:Mastitis, Bovine / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D011525:Prototheca / Q000235:genetics* / Q000472:pathogenicity*; D012337:RNA, Ribosomal, 18S / Q000235:genetics
D017931:DNA Primers; D012337:RNA, Ribosomal, 18S