title: Mass spectrometry and scanning electron microscopy study of silicone tunneled dialysis catheter integrity after an exposure of 15 days to 60% ethanol solution.
abstract: Anti-infectious lock is an emerging therapeutic option for preventing and/or controlling catheter-associated infection. Ethanol has widespread bactericidal activity, limited side effects, and low risk of inducing antimicrobial resistance. However, concerns have been raised about ethanol-induced catheter structural degradation. In this study, silicone catheters were immersed at 37 degrees C in three different solvents: 0.9% sodium chloride, 60% ethanol, and 95% ethanol for 4 h, 15 days and 15 days after a first storage of 4 h. Scanning electron microscopy (magnification 1000-20 000 times) of the inner surface of the catheter revealed no damage to the lumen surfaces of catheters immersed in 95% ethanol for 15 days compared with the reference catheter. Gas chromatography/mass spectrometry (GC/MS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) analysis of the storage solutions revealed a significant release of polydimethylsiloxanes having a number of dimethylsiloxane units lower than 30 in the 95% ethanol solution and a structure highly consistent with a cyclic structure. Most release occurred within the first 4 h of exposure. In contrast, there was no difference in the small amounts of silicone released in 0.9% sodium chloride as reference and 60% ethanol solution, whatever the exposure time. These results should allow the development of clinical trials to assess the efficacy of the 60% ethanol lock technique in preventing or controlling the infectious complications of silicone dialysis catheters.
authors.affiliation: University of Clermont 1, Laboratoire de Chimie Analytique et Spectrométrie de Masse, UFR Pharmacie, 63001 Clermont-Ferrand, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Guenu
authors.forename: Anne-Elisabeth
authors.initials: AE
authors.lastname: Heng
authors.forename: Françoise
authors.initials: F
authors.lastname: Charbonné
authors.forename: Marie-Josèphe
authors.initials: MJ
authors.lastname: Galmier
authors.forename: Franck
authors.initials: F
authors.lastname: Charlès
authors.forename: Patrice
authors.initials: P
authors.lastname: Deteix
authors.forename: Bertrand
authors.initials: B
authors.lastname: Souweine
authors.forename: Claire
authors.initials: C
authors.lastname: Lartigue
mesh_terms: D000891:Anti-Infective Agents, Local / Q000737:chemistry*; D002408:Catheters, Indwelling; D000431:Ethanol / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D006435:Renal Dialysis / Q000295:instrumentation*; D012833:Siloxanes / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000891:Anti-Infective Agents, Local; D012833:Siloxanes; D000431:Ethanol