Specific recognition of the basic N peptide by an RNA.
Artificial binding model systems with sufficient specificity are indispensable for study and understanding of living systems. Here, we report a small hairpinloop RNA that interacts with a basic peptide derived from bacteriophage lambda N protein, as a new protein-RNA binding model. The RNA was selected in vitro from a hairpinloop RNA library and recognized the target N peptide more specifically than the boxB RNA, the naturally occurring N protein binder.
Kawakami
Junji
J
Frontier Institute for Biomolecular Engineering Research, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Tokitoh
Hisanori
H
Tanabe
Yoshiatsu
Y
Sugimoto
Naoki
N
D000595:Amino Acid Sequence; D007700:Kinetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D054334:Viral Regulatory and Accessory Proteins / Q000737:chemistry*
C059417:N protein, Bacteriophage lambda; D010455:Peptides; D054334:Viral Regulatory and Accessory Proteins; D012313:RNA