[Tyroservatide inhibits the growth of human hepatocarcinoma in nude mice].
OBJECTIVE
To investigate the inhibitory effects of tyroservatide and its amino acid mixture on growth of hepatocarcinoma.


METHODS
Hepatocarcinoma in nude mice was induced by implantation of cells of human hepatocarcinoma cell line BEL-7402. The inhibition of hepatocarcinoma growth was determined by calculating the tumor volume and measuring the tumor weight. The effects of tyroservatide on tumor cells in nude mice were assessed by immunohistochemical staining of proliferating cell nuclear antigen (PCNA), electron microscopic observation of ultrastructure, and apoptosis of tumor cells using terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL).


RESULTS
Tyroservatide significantly inhibited the growth of human hepatocarcinoma in nude mice, with an inhibiting rate more than 60%. But the mixture of amino acid did not show a significant inhibitory effect on the tumor growth. Tyroservatide also induced apoptosis of tumor cells and decreased the expression of PCNA in tumor cells.


CONCLUSIONS
Tyroservatide may significantly inhibit the growth of human hepatocarcinoma in nude mice by inducing apoptosis and inhibiting proliferation of tumor cells.
Jia
Jung
J
Department of Immunology, Tianjin Medical University, Tianjin 300070, China.
Lu
Rong
R
Fu
Zheng
Z
Qiu
Shuang
S
Shi
Lin-Xi
LX
Jian
Xu
X
Liu
Jun-Yan
JY
Li
Hui-Qiang
HQ
Che
Xu-Chun
XC
Yao
Zhi
Z
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009842:Oligopeptides / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; D009842:Oligopeptides; D018809:Proliferating Cell Nuclear Antigen; C510683:tripeptide tyroservatide