Natural polymorphisms of HIV type 2 pol sequences from drug-naive individuals.
Until today, the susceptibility of human immunodeficiency virus type 2 (HIV-2) to protease and nucleosidic reverse-transcriptase inhibitors (PI and NRTI, respectively) has not been clearly documented. In this report we studied HIV-2 proviral sequences (n = 30) from drug-naive patients. Our results revealed that several amino acid positions in the protease and reverse transcriptase coding sequence harbored residues that have been associated with drug resistance in HIV-1-infected patients. In particular, the M46I substitution in the protease was detected in 90% of the sequences analyzed, which, together with the other substitutions identified, may indicate a reduced susceptibility of HIV-2-infected drug-naive patients to PI. Furthermore, interpretation of genotypic data with four available algorithms, developed for interpretation of HIV-1 sequence data, suggested nonoverlapping profiles of drug resistance.
Parreira
Ricardo
R
Unidade de Virologia/Unidade de Parasitologia e Microbiologia Médicas, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, P-1349-008 Lisboa, Portugal. Ricardo@ihmt.unl.pt
Monteiro
Filipa
F
Pádua
Elizabeth
E
Piedade
João
J
Venenno
Teresa
T
Paixão
Maria Teresa
MT
Esteves
Aida
A
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D019380:Anti-HIV Agents / Q000627:therapeutic use; D024921:Drug Resistance, Multiple, Viral; D015684:Gene Products, pol / Q000235:genetics*; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology*; D015498:HIV-2 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics*
D019380:Anti-HIV Agents; D015684:Gene Products, pol