Design of base-discriminating fluorescent (BDF) nucleobase for SNP typing.
We have devised novel pyrene-labeled BDF nucleosides, (Py)U, (Py)C, (Py)deazaA, and (Py)A. BDF probes containing these fluorescent nucleosides selectively emit fluorescence only when the base opposite the BDF base is a target base. Oligonucleotides containing these BDF nucleosides act as effective reporter probes for homogeneous SNP typing of DNA samples.
Saito
Yoshio
Y
NEWCAT Institute, Faculty of Engineering, Nihon University and SORST, Japan Science and Technology Corporation, Tamura, Koriyama 963-8642, Japan.
Kanatani
Keiichiro
K
Ochi
Yuji
Y
Okamoto
Akimitsu
A
Saito
Isao
I
D005456:Fluorescent Dyes / Q000737:chemistry*; D024522:Genes, BRCA2; D006801:Humans; D015345:Oligonucleotide Probes / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes