title: Prototheca zopfii genotype 2: the causative agent of bovine protothecal mastitis?
abstract: In order to clarify the epidemiology of bovine protothecal mastitis, 30 Prototheca zopfii mastitis isolates were genetically investigated. Based on the 18S rDNA, which allows a differentiation of the former species P. zopfii in two distinct P. zopfii genotypes and Prototheca blaschkeae sp. nov., newly developed genotype-specific PCR-assays as well as RFLP-assays were applied. All mastitis isolates investigated could be assigned to P. zopfii genotype 2 suggesting that this genotype is the aetiological agent of bovine Prototheca mastitis.
authors.affiliation: Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierklinken 1, D-04103 Leipzig, Germany.
authors.forename: Asja
authors.initials: A
authors.lastname: Möller
authors.forename: Uwe
authors.initials: U
authors.lastname: Truyen
authors.forename: Uwe
authors.initials: U
authors.lastname: Roesler
mesh_terms: D000818:Animals; D002417:Cattle; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005838:Genotype; D005858:Germany / Q000453:epidemiology; D007239:Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008414:Mastitis, Bovine / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D011525:Prototheca / Q000235:genetics* / Q000472:pathogenicity*; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D017931:DNA Primers; D012337:RNA, Ribosomal, 18S