title: Screening of modified DNA aptamers that recognize DNA secondary structure.
abstract: We attempted to utilize in vitro selection techniques to develop modified DNA aptamers that are specific for DNA secondary structure including a single-mismatch base pair. For the selection, two kinds of random modified DNA library were prepared by an enzymatic method using modified 2'-deoxynucleoside 5'-triphosphates and KOD Dash DNA polymerase: one library consists of modified DNA including amino functionality and another consists of modified DNA including both hydroxypropynyl and guanidium functionalities.
authors.affiliation: Department of Applied Chemistry, Gunma University, Kiryu, Gunma 376-8515, Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kasamatsu
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shoji
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ozaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sawai
mesh_terms: D052157:Aptamers, Nucleotide / Q000302:isolation & purification*; D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D015723:Gene Library; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D004247:DNA; D004259:DNA-Directed DNA Polymerase