Folding transition of DNA depending on ionic environments.
We examined the effect of monovalent salts, NaCl and KCl, on spermidine-induced DNA compaction using fluorescence microscopy. By increasing concentrations of salts, unfolding process of compacted DNAs were observed. It was also shown that NaCl is more effective for unfolding compacted DNAs than KCl.
Hibino
Kumiko
K
Department of Food and Nutrition, Nagoya Bunri College, Nagoya 451-0077, Japan.
Yoshikawa
Yuko
Y
Murata
Shizuaki
S
D017122:Bacteriophage T4 / Q000737:chemistry / Q000187:drug effects; D004279:DNA, Viral / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D011189:Potassium Chloride / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*; D012996:Solutions
D004279:DNA, Viral; D012996:Solutions; D012965:Sodium Chloride; D011189:Potassium Chloride