Recognition of DNA with assembly of minor groove binders mediated by metal complexation.
Genome-targeting molecules would have broad application as probes in genomic study. We recently developed the ligands (Bpy-H) connecting the Hoechst33258 skeleton for DNA binding and 2,2'-bipyridine for Cu(2+) complexation. It was shown that L-Bpy-H with a long linker exhibited Cu(2+)-mediated assembly on the DNA template having two A(3)T(3) sites in a ratio of 1:2:1 DNA-ligand-Cu(2+). Binding selectivity depends on the distance between the two A(3)T(3) sites of DNA. In contrast, S-Bpy-H having a short linker tended to form 1:2:2 DNA-ligand-Cu(2+) complex.
Kataoka
Tomonobu
T
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Tanada
Mikimasa
M
Onishi
Ippei
I
Sasaki
Shigeki
S
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D001562:Benzimidazoles / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D008024:Ligands; D009690:Nucleic Acid Conformation
D001562:Benzimidazoles; C522088:L-Bpy-H compound; D008024:Ligands; C522089:S-Bpy-H compound; D015082:2,2'-Dipyridyl; D003300:Copper; D004247:DNA