title: Cloning and characterization of a gene encoding the human putative ubiquitin conjugating enzyme E2Z (UBE2Z).
abstract: Ubiquitination, the covalent attachment of the protein ubiquitin (Ub) to other cellular proteins, has been implicated in a number of important physiological processes. ubiquitin conjugating enzyme (E2) plays an important role in the ubiquitin system. Here we report the research about a human putative ubiquitin conjugating enzyme gene, UBE2Z. The length of UBE2Z cDNA is 3054 base pairs and contains an open reading frame encoding 246 amino acids. The UBE2Z gene was mapped to human chromosome 17q21.32 and consisted of 6 exons. RT-PCR showed that UBE2Z was widely expressed in human tissues, especially high in placenta, pancreas, spleen and testis. The UBE2Z-GFP fusion protein was located in both nucleus and cytoplasm of AD293 cells.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Gu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Mei
authors.initials: M
authors.lastname: Zheng
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Fei
authors.forename: Xudong
authors.initials: X
authors.lastname: Chen
authors.forename: Shengdong
authors.initials: S
authors.lastname: Huang
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics* / Q000378:metabolism
chemical_list: C525243:UBE2Z protein, human; D044763:Ubiquitin-Conjugating Enzymes