[Expression of CD66c (CEACM6) in adult acute leukemia and its significance].
OBJECTIVE
To explore the expression of CD66c (CEACM6) in adult acute leukemia and its significance.


METHODS
Acute leukemia cell lines HL-60, K562, LCL721.221 and Jurkat were cultured in vitro. RT-PCR and multi-parameter flow cytometry were applied to analysis of CD66c mRNA and protein expression respectively in the cell lines and patient' s bone marrow leukemic cells. Cytogenetic analysis for 199 bone marrow samples from leukemia patients and Minimal Residual Disease (MRD) detection for 25 CD66c positive B lineage ALL were performed.


RESULTS
(1) CD66c expression both on cell surface and in plasma were negative in all the cell lines. (2) Four of 127 AML (3.15%) (mainly of M2 and M4), and 28 of 79 ALL (35.44%) (all of B linage ALL) were CD66c positive the subtypes of the ALL being common B-ALL (20/54) and pre B-ALL (8/11) including 8 Ph + B-linage ALL. (3) Six-month relapse rate was significantly different between the MRD positive and negative patients. (4) CD66c mRNA was strongly expressed in B-linage ALL. For the cell lines, only the HL60 cells weakly expressed CD66c mRNA.


CONCLUSIONS
CD66c expression could be a useful bio-marker for the MRD analysis in ALL, and is closely associated with its transcription level.
Chen
Bao-guo
BG
Central laboratory,Department of hematology of Affialiated Taizhou Hospital, Wenzhou Medical College, Linhai 317000, China.
Yan
Wei-hua
WH
Meng
Zhe-feng
ZF
Guo
Zong-ming
ZM
Zhu
Min
M
Li
Bo-li
BL
D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis*; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis* / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D058851:GPI-Linked Proteins; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis
D015703:Antigens, CD; C504117:CEACAM6 protein, human; D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D012333:RNA, Messenger