title: Mechanism of action of DNA-hydrolyzing antibodies to DNA from blood of patients with systemic lupus erythematosus.
abstract: Four fractions of IgG antibodies to native DNA (nDNA) were obtained from blood of patients with systemic lupus erythematosus (SLE). These antibodies displayed a thermostable DNA-hydrolyzing activity and were different in affinity for DNA-cellulose and sorption on DEAE-cellulose. DNA-hydrolyzing antibodies to nDNA are metal-dependent endonucleases, cause mainly single-strand breaks in DNA, and are active over a wide range of pH. By atomic-force microscopy, three-dimensional images of DNA complexes with DNA-hydrolyzing antibodies to nDNA were obtained with nanometer resolution, and a nonprocessive action mechanism was shown for the DNase activity of antibodies to nDNA.
authors.affiliation: Kazan State University, 420008 Kazan, Russia. Tatyana.Nevzorova@ksu.ru
authors.forename: T A
authors.initials: TA
authors.lastname: Nevzorova
authors.forename: V G
authors.initials: VG
authors.lastname: Vinter
authors.forename: O A
authors.initials: OA
authors.lastname: Konovalova
authors.forename: M Kh
authors.initials: MKh
authors.lastname: Salakhov
mesh_terms: D000293:Adolescent; D000328:Adult; D000974:Antibodies, Antinuclear / Q000097:blood / Q000494:pharmacology*; D000915:Antibody Affinity; D002482:Cellulose / Q000031:analogs & derivatives / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004270:DNA, Circular / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006868:Hydrolysis; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged
chemical_list: D000974:Antibodies, Antinuclear; D004270:DNA, Circular; C033190:DNA-cellulose; D002482:Cellulose; D004247:DNA; D003851:Deoxyribonucleases