SNPs analysis by use of oligonucleotides containing 2'-O-methyl-2-thiouridines.
In this study, we report an improved method for the synthesis of oligonucleotides containing 2-thiouridine (s2U) derivatives. The hybridization affinity and base pair recognition ability of oligonucleotides having 2-thiouracil moieties with both the complementary RNAs and DNAs were also studied in detail. These results indicate that oligonucleotides containing s2U derivatives could be used as powerful tools for chip-based SNPs detection.
Okamoto
Itaru
I
Graduate School of Life Science, Tokyo Institute of Technology.
Seio
Kohji
K
Sekine
Mitsuo
M
D020029:Base Pairing; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000737:chemistry
C409967:2'-O-methyl-2-thiouridine; D015345:Oligonucleotide Probes; D013891:Thiouridine