[Proteomic analysis of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells].
OBJECTIVE
To investigate the mechanism of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells.


METHODS
In this study, the proteins extracted from paclitaxel-induced apoptotic MCF-7 cells were analyzed by 2-dimentional gel electrophoresis (2-DE), and compared with those from untreated MCF-7 cells. The differential proteins were identified by mass spectrometry.


RESULTS
At 24 hour after paclitaxel (100 nmol/L) treatment, MCF-7 cells were collected and extracted the whole proteins. Seventeen up-regulated or down-regulated proteins were found by analysis of the differential proteomic 2-DE map. Six of them were identified by mass spectrometry. They were enolase 1, chloride intracellular channel 1, keratin 8, ribosomal protein S12, galectin-1 and histidine triad nucleotide binding protein, respectively.


CONCLUSIONS
We effectively found the changed proteins in the process of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells by proteomic techniques. These up-regulated or down-regulated proteins are important molecules for our further research about the mechanism of paclitaxel-induced apoptosis.
Wu
Feng
F
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Wang
Qing-Ming
QM
Fan
Guo-Cai
GC
Chen
Ji-Zhong
JZ
Chen
Hui-Peng
HP
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D037483:Galectin 1 / Q000378:metabolism; D006801:Humans; D053533:Keratin-8 / Q000378:metabolism; D017239:Paclitaxel / Q000494:pharmacology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012269:Ribosomal Proteins / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D037483:Galectin 1; D053533:Keratin-8; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D010751:Phosphopyruvate Hydratase; D017239:Paclitaxel