Ligand-binding activity and expression profile of annexins in Caenorhabditis elegans.
Mammalian annexins are implicated in several physiological mechanisms based on their calcium-dependent phospholipid/membrane binding and carbohydrate-binding activities. In this study, we investigated gene expression profiles of all four Caenorhabditis elegans annexins, nex-1, -2, -3 and -4, throughout the development, and compared phospholipid- and carbohydrate-binding properties of their protein products, NEX-1, -2, -3 and -4. We found that nex-1 and -3 are transcribed continuously during the developmental stages, while expression of nex-2 and -4 appeared to be temporal, peaking at the L1 stage followed by a gradual decrease toward the adult stage. NEX-1 and -3 were detected as single protein band in total worm extracts by immunoblotting, but NEX-2 was heterogenic in size. NEX-1, -2, and -3 showed the binding activities to phosphatidylserine, phosphatidylinositol and phosphatidylethanolamine, but not to phosphatidylcholine. In contrast to their uniform phospholipids-binding properties, their glycosaminoglycan-binding activities were distinctive. NEX-2 bound to heparan sulfate and chondroitin, NEX-3 bound only to heparan sulfate, and NEX-1 showed no lectin activities under tested conditions. NEX-4 had neither phospholipids- nor carbohydrate-binding properties. Differentiated expression profiles and ligand-binding properties of NEX-1, -2, -3 and -4, shown in our study, may represent distinctive roles for each C. elegans annexins.
Nishioka
Sara
S
Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan.
Aikawa
Jun-ichi
J
Ida
Michiru
M
Matsumoto
Isamu
I
Street
Miyoko
M
Tsujimoto
Masafumi
M
Kojima-Aikawa
Kyoko
K
D000595:Amino Acid Sequence; D000818:Animals; D017302:Annexins / Q000096:biosynthesis / Q000378:metabolism*; D017173:Caenorhabditis elegans / Q000254:growth & development / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000096:biosynthesis / Q000378:metabolism*; D002807:Chondroitin / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006497:Heparan Sulfate / Q000378:metabolism; D015151:Immunoblotting; D008081:Liposomes / Q000378:metabolism; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
D017302:Annexins; D029742:Caenorhabditis elegans Proteins; D002807:Chondroitin; D006497:Heparan Sulfate; D008081:Liposomes; D010743:Phospholipids; C098890:Nex-1 protein, C elegans; C519288:Nex-2 protein, C elegans; C519289:Nex-3 protein, C elegans; C519290:Nex-4 protein, C elegans