Long-term lowering of tumour interstitial fluid pressure reduces Ki-67 expression.
High tumour interstitial fluid pressure (TIFP) is a characteristic of most solid tumours. Recent data give first evidence that mechanical stretch induced by TIFP triggers proliferation in solid tumours. In the present study we compared two protocols of TIFP reduction on the expression of the tumour proliferation marker Ki-67: (a) short-term lowering of TIFP by a singular puncture and (b) long-term lowering of TIFP by catheterization. Utilizing two experimental tumours (A431, A549) it was found that the TIFP broke down rapidly after a singular puncture but recovered within 6h. In case of permanent catheterization no TIFP recovery was observed. After 24h tumours were excised and stained against the proliferation marker Ki-67. While a singular puncture had no effect catheterized tumours showed a significant decrease in Ki-67 expression. Our data suggest that long-term lowering of TIFP is required to reduce tumour proliferation.
Hofmann
Matthias
M
Department of Dermatology and Venerology, Johann Wolfgang Goethe-University, Theodor-Stern Kai 7, D-60590 Frankfurt/Main, Germany. Matthias.Hofmann@em.uni-frankfurt.de
Schultz
Maike
M
Bernd
August
A
Bereiter-Hahn
Jürgen
J
Kaufmann
Roland
R
Kippenberger
Stefan
S
D000818:Animals; D002404:Catheterization; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D045604:Extracellular Fluid / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D011312:Pressure
D019394:Ki-67 Antigen; C498355:Mki67 protein, mouse