Hydrogenosomal activity of Trichomonas vaginalis cultivated under different iron conditions.
To evaluate whether iron concentration in TYM medium influence on hydrogenosomal enzyme gene expression and hydrogenosomal membrane potential of Trichomonas vaginalis, trophozoites were cultivated in irondepleted, normal and iron-supplemented TYM media. The mRNA of hydrogenosomal enzymes, such as pyruvate ferredoxin oxidoreductase (PFOR), hydrogenase, ferredoxin and malic enzyme, was increased with iron concentrations in T. vaginalis culture media, measured by RT-PCR. Hydrogenosomal membrane potentials measured with DiOC6 also showed similar tendency, e.g. T. vaginalis cultivated in iron-depleted and iron-supplemented media for 3 days showed a significantly reduced and enhanced hydrogenosomal membrane potential compared with that of normal TYM media, respectively. Therefore, it is suggested that iron may regulate hydrogenosomal activity through hydrogenosomal enzyme expression and hydrogenosomal membrane potential.
Kim
Yong-Seok
YS
Department of Biochemistry and Molecular Biology, Hanyang University College of Medicine, Seoul, Korea.
Song
Hyun-Ouk
HO
Choi
Ik-Hwa
IH
Park
Soon-Jung
SJ
Ryu
Jae-Sook
JS
D000818:Animals; D003470:Culture Media; D005288:Ferredoxins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000235:genetics / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D008564:Membrane Potentials; D015388:Organelles / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology*; D050743:Pyruvate Synthase / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014246:Trichomonas vaginalis / Q000254:growth & development*
D003470:Culture Media; D005288:Ferredoxins; D006859:Hydrogen; D007501:Iron; D008291:Malate Dehydrogenase; D006864:Hydrogenase; D050743:Pyruvate Synthase