Electrochemical detection of DNA single base mismatch by the use of strand exchange reaction.
The electrochemical method based on the strand exchange reaction (SER) for the detection of DNA single-base mismatch has been developed. Different electrochemical responses due to the slower SER rates for mismatch containing DNA than fully matched DNA were observed by using the redox-modified partial duplex DNA immobilized on gold electrode as an analytical probe.
Kumamoto
Satoshi
S
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Syosya, Himeji 671-2201, Japan.
Maruyama
Atsushi
A
Nakamura
Mitsunobu
M
Yamana
Kazushige
K
D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D004252:DNA Mutational Analysis / Q000379:methods*; D015342:DNA Probes; D004563:Electrochemistry; D004566:Electrodes; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction
D015342:DNA Probes