Rapid pulsed-field gel electrophoresis protocol for subtyping of Streptococcus suis serotype 2.
A rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping of Streptococcus suis serotype 2 was developed and evaluated using 27 clinical isolates from 22 epidemiologically unrelated patients. Results were matched against antibiogram, virulence genotyping and multi locus sequence typing (MLST). PFGE appeared to be the most discriminatory with numerical index of discrimination (D) equal to 0.87.
Luey
Cindy K Y
CK
Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong SAR, China.
Chu
Yiu Wai
YW
Cheung
Terence K M
TK
Law
Catherine C P
CC
Chu
Man Yu
MY
Cheung
Danny T L
DT
Kam
Kai Man
KM
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D008826:Microbial Sensitivity Tests; D012703:Serotyping; D017011:Streptococcus suis / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity; D013997:Time Factors; D014774:Virulence / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial