title: Histocompatible embryonic stem cells by parthenogenesis.
abstract: Genetically matched pluripotent embryonic stem (ES) cells generated via nuclear transfer or parthenogenesis (pES cells) are a potential source of histocompatible cells and tissues for transplantation. After parthenogenetic activation of murine oocytes and interruption of meiosis I or II, we isolated and genotyped pES cells and characterized those that carried the full complement of major histocompatibility complex (MHC) antigens of the oocyte donor. Differentiated tissues from these pES cells engrafted in immunocompetent MHC-matched mouse recipients, demonstrating that selected pES cells can serve as a source of histocompatible tissues for transplantation.
authors.affiliation: Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA.
authors.forename: Kitai
authors.initials: K
authors.lastname: Kim
authors.forename: Paul
authors.initials: P
authors.lastname: Lerou
authors.forename: Akiko
authors.initials: A
authors.lastname: Yabuuchi
authors.forename: Claudia
authors.initials: C
authors.lastname: Lengerke
authors.forename: Kitwa
authors.initials: K
authors.lastname: Ng
authors.forename: Jason
authors.initials: J
authors.lastname: West
authors.forename: Andrew
authors.initials: A
authors.lastname: Kirby
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Daly
authors.forename: George Q
authors.initials: GQ
authors.lastname: Daley
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D020090:Chromosome Segregation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D005260:Female; D005838:Genotype; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology*; D006579:Heterozygote; D006648:Histocompatibility; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D008285:Major Histocompatibility Complex; D008540:Meiosis; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009865:Oocytes / Q000166:cytology / Q000276:immunology; D010312:Parthenogenesis; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D033581:Stem Cell Transplantation
chemical_list: D006183:H-2 Antigens; C489629:H-2A(b) antigen, mouse; C024755:H-2K(K) antigen; D000949:Histocompatibility Antigens Class II