Electrochemical gene detection by using adamantyl naphthalene diimide coupled with ferrocenyl-beta-cyclodextrin.
The adamantyl moieties of adamantylnaphthalene diimide (AND) can be incorporated into the cavity of ferrocenyl-beta-cyclodextrin even where they are bound to double stranded DNA by threading intercalation. When this ternary complex is formed on the DNA probe-immobilized electrode, electrochemical DNA detection is feasible: the electrode hybridized with target DNA gave rise to a current peak corresponding to the ferrocene oxidation upon treatment with AND and ferrocenyl-beta-cyclodextrin (Fc-beta-CD).
Sato
Shinobu
S
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812-8581, Japan.
Nojima
Takahiko
T
Kondo
Hiroki
H
Takenaka
Shigeori
S
D000218:Adamantane / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004563:Electrochemistry; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry*; D005796:Genes; D006046:Gold; D006863:Hydrogen-Ion Concentration; D007094:Imides / Q000737:chemistry*; D000075163:Metallocenes; D009281:Naphthalenes; D010618:Phenanthrolines / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
D005296:Ferrous Compounds; D007094:Imides; D000075163:Metallocenes; D009281:Naphthalenes; D010618:Phenanthrolines; C521147:adamantyl naphthalene diimide; D047392:beta-Cyclodextrins; C542131:naphthalenediimide; D006046:Gold; D004247:DNA; C031215:betadex; D000218:Adamantane; C004998:ferrocene