title: [Design of a microfluidic immunoassay system based on superparamagnetic microbeads].
abstract: We use direct-write laser micromachining technology to fabricate the microfluidic chip, and to establish a microfluidic chemiluminescence immunoassay system based on superparamagnetic microbeads, for detecting alpha- fetoprotein (AFP). The AFP analysis can be completed in 20 minutes with 5 microl sample and 5 microl reagent, and there is a good linear correlation in the range of 1-800 ng/ml.
authors.affiliation: Key Lab of Biomechanics and Tissue Engineering, State Education Ministry, Bioenginering College, Chongqing University, Chongqing.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Zheng
authors.forename: Jing
authors.initials: J
authors.lastname: Pan
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Zhao
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Pu
mesh_terms: D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D000509:alpha-Fetoproteins