title: Genome wide responses of murine lungs to dietary alpha-tocopherol.
abstract: Alpha-tocopherol (alpha-T) may affect biological processes by modulating mRNA concentrations. This study screened the responses of approximately 15,000 lung mRNAs to dietary alpha-T in mice. The lung was chosen as the target organ because it is subjected to cyclical variations in oxidant and inflammatory stressors and alpha-T has been implicated in their modulations. The analysis identified approximately 400 mRNAs sensitive to alpha-T status of lungs determined by dietary alpha-T. The female lung transcriptome appears to be more sensitive to the alpha-T status than that of the male lungs. Here, we focus on the induction of 13 cytoskeleton genes by dietary alpha-T because they were similarly induced in the male and the female lungs. Their inductions were confirmed by quantitative-real-time-polymerase chain reaction (qRT-PCR). Immunohistochemical analyses of three of the encoded proteins suggest that they are expressed in lung vasculature and alveolar regions. The data suggest that the lung alpha-T status may modulate cytoarchitecture of lungs.
authors.affiliation: Division of Pulmonary and Critical Care, Department of Internal Medicine, Genome and Biomedical Sciences Facility, University of California, Davis, CA 95616, USA.
authors.forename: Saji
authors.initials: S
authors.lastname: Oommen
authors.forename: Vihas T
authors.initials: VT
authors.lastname: Vasu
authors.forename: Scott W
authors.initials: SW
authors.lastname: Leonard
authors.forename: Maret G
authors.initials: MG
authors.lastname: Traber
authors.forename: Carroll E
authors.initials: CE
authors.lastname: Cross
authors.forename: Kishorchandra
authors.initials: K
authors.lastname: Gohil
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D004032:Diet; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D008168:Lung / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D024502:alpha-Tocopherol / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D024502:alpha-Tocopherol