Quantum dots for human mesenchymal stem cells labeling. A size-dependent autophagy activation.
Lately certain cytotoxicity of quantum dots (QDs) and some deleterious effects of labeling procedure on stem cells differentiation abilities were shown. In the present study we compared cytotoxicity and intracellular processing of two different-sized protein-conjugated QDs after labeling of the human mesenchymal stem cells (hMSC). An asymmetrical intracellular uptake of red (605 nm) and green (525 nm) quantum dots was observed. We describe for the first time a size-dependent activation of autophagy, caused by nanoparticles.
Seleverstov
Oleksandr
O
Center for Biotechnology and Biomedicine (BBZ), University of Leipzig, D-04103 Leipzig, Germany. alex_seleverstov@yahoo.de
Zabirnyk
Olga
O
Zscharnack
Matthias
M
Bulavina
Larysa
L
Nowicki
Marcin
M
Heinrich
Jan-Michael
JM
Yezhelyev
Maksym
M
Emmrich
Frank
F
O'Regan
Ruth
R
Bader
Augustinus
A
D001343:Autophagy; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D005453:Fluorescence; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D045663:Quantum Dots
C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins