[Construction of HESR1 siRNA plasmid expression vector].
OBJECTIVE
To construct of HESR1 siRNA plasmid expression vector.


METHODS
Cultures human umbilicus vein endothelial cell, according to the computer aided design, HESR1-specific siRNA gene was synthesized and cloned into the RNAi-Ready pSIREN-RetroQ ZsGreen Vector. The constructed HESR1-RNAi plasmid were transfected into human umbilicus vein endothelial cell, RT-PCR detected the effected of HESR1-RNAi plasmid.


RESULTS
The recombinant was cloned and the sequence was obtained, HESR1-RNAi plasmid can down-regulated the expression of HESR1 in human umbilicus vein endothelial cell. Conclusion : Successfully cloning the recombinant make it possible searching new mechanism of HESRI in angiogenesis.
Li
Bin
B
Zhongshan Ophthalmic Center, Sun Yat-sen University Guangzhou 510060, China.
Tang
Shibo
S
Lin
Shaofen
S
Meng
Jing
J
Xiao
Ying
Y
D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection; D014471:Umbilical Veins / Q000166:cytology
D051792:Basic Helix-Loop-Helix Proteins; D034741:RNA, Small Interfering