Interaction between NifL and NifA in the nitrogen-fixing Pseudomonas stutzeri A1501.
Pseudomonas stutzeri strain A1501 isolated from rice fixes nitrogen under microaerobic conditions in the free-living state. This paper describes the properties of nifL and nifA mutants as well as the physical interaction between NifL and NifA proteins. A nifL mutant strain that carried a mutation non-polar on nifA expression retained nitrogenase activity. Complementation with a plasmid containing only nifL led to a decrease in nitrogenase activity in both the wild-type and the nifL mutant, suggesting that NifL acts as an antiactivator of NifA activity. Using the yeast two-hybrid system and purified protein domains of NifA and NifL, an interaction was shown between the C-terminal domain of NifL and the central domain of NifA, suggesting that NifL antiactivator activity is mediated by direct protein interaction with NifA.
Xie
Zhihong
Z
Biotechnology Research Institute, CAAS, Beijing, PR China.
Dou
Yuetang
Y
Biotechnology Research Institute, CAAS, Beijing, PR China.
Ping
Shuzheng
S
Biotechnology Research Institute, CAAS, Beijing, PR China.
Chen
Ming
M
Biotechnology Research Institute, CAAS, Beijing, PR China.
Wang
Guoying
G
Biology College, China Agricultural University, Beijing, PR China.
Elmerich
Claudine
C
Institut des Sciences du Végétal, CNRS UPR-2355, Gif-sur-Yvette, France.
Lin
Min
M
Biotechnology Research Institute, CAAS, Beijing, PR China.
D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005816:Genetic Complementation Test; D009154:Mutation; D009586:Nitrogen Fixation / Q000235:genetics; D009591:Nitrogenase / Q000032:analysis; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D044223:Pseudomonas stutzeri / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D001426:Bacterial Proteins; C061338:NifA protein, Bacteria; D014157:Transcription Factors; C084296:nifL protein, Bacteria; D009591:Nitrogenase