Structural requirements of signal peptide in insect cell.
In order to increase the proteinic secretion quantity of production in insect cell, the N-terminal and the hydrophobic core region of the chicken lysozyme signal peptide (CLSP) was altered. A hybrid gene composed of CLSP gene and human lysozyme (HLY) gene from short DNA oligomers was used for the production of recombinant HLY in insect cell using vacuole virus expression system. In contrast with the results in yeast, the introduction of basic residues in the N-terminal region did not make the amount of secretion increase in insect cell. On the other hand, when the sequence of CLSP was changed into Leu-rich type, the amount of HLY secretion increased sharply. Although they are eukaryotes, the structural requirements of signal peptides may have some differences in yeast and insect cell.
Tsuchiya
Yoshinori
Y
Department of Exotic Diseases, National Institute of Animal Health, Jyosuihoncho 6-20-1, Kodaira, Tokyo 187-0022, Japan.
Morioka
Kazuki
K
Shirai
Jyunsuke
J
Yoshida
Kazuo
K
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D006801:Humans; D007313:Insecta / Q000166:cytology*; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D021382:Protein Sorting Signals; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013329:Structure-Activity Relationship
D021382:Protein Sorting Signals; D009113:Muramidase