title: Detection of a small number of Cryptosporidium parvum oocysts by sugar flotation and sugar centrifugation methods.
abstract: Detection rates from the samples including a small number of Cryptosporidium parvum oocysts were compared between the sugar flotation and the sugar centrifugal flotation methods. As the results, the oocysts were detected from 70 and 80 of 100 samples including 6.0x10(2) and 1.0x10(3) oocysts per 1 ml by the flotation method, respectively, whereas from 52 and 53 of the same samples by the centrifugal flotation method. Therefore, it was considered that the flotation method is the most suitable method for the detection from samples including a small number of Cryptosporidium oocysts. It is also suggested that results of the sugar flotation method were reliable for samples including more than 1.0x10(3) oocysts/ml.
authors.affiliation: Division of Tropical Diseases and Parasitology, Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujino
authors.forename: Tomohide
authors.initials: T
authors.lastname: Matsuo
authors.forename: Manami
authors.initials: M
authors.lastname: Okada
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Matsui
mesh_terms: D000818:Animals; D002498:Centrifugation; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D033661:Oocysts / Q000166:cytology*; D010270:Parasite Egg Count / Q000379:methods*; D013046:Specific Gravity; D013395:Sucrose; D014871:Water Microbiology
chemical_list: D013395:Sucrose