Frequent hypermethylation of the DNA repair gene MGMT in long-term survivors of glioblastoma multiforme.
We have performed a methylation-specific PCR approach to comparatively analyze the MGMT promoter methylation status in 186 glioblastomas (GBM) from patients with classic survival and nine from patients with long-term survival (LTS GBM). The methylation rate in LTS GBM was significantly higher (77.8% vs. 39.2%, P = 0.033) which suggests that MGMT hypermethylation is a frequent hallmark of LTS GBM and contributes to characterize this intriguing GBM subtype.
Martinez
Ramon
R
Schackert
Gabriele
G
Yaya-Tur
Ricard
R
Rojas-Marcos
Iñigo
I
Herman
James G
JG
Esteller
Manel
M
D000328:Adult; D000368:Aged; D016543:Central Nervous System Neoplasms / Q000235:genetics* / Q000503:physiopathology; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000235:genetics*; D045643:DNA Repair Enzymes / Q000235:genetics*; D005909:Glioblastoma / Q000235:genetics* / Q000503:physiopathology; D006801:Humans; D008875:Middle Aged; D016019:Survival Analysis; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D025521:Tumor Suppressor Proteins; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes