Genome wide responses of murine lungs to dietary alpha-tocopherol.
Alpha-tocopherol (alpha-T) may affect biological processes by modulating mRNA concentrations. This study screened the responses of approximately 15,000 lung mRNAs to dietary alpha-T in mice. The lung was chosen as the target organ because it is subjected to cyclical variations in oxidant and inflammatory stressors and alpha-T has been implicated in their modulations. The analysis identified approximately 400 mRNAs sensitive to alpha-T status of lungs determined by dietary alpha-T. The female lung transcriptome appears to be more sensitive to the alpha-T status than that of the male lungs. Here, we focus on the induction of 13 cytoskeleton genes by dietary alpha-T because they were similarly induced in the male and the female lungs. Their inductions were confirmed by quantitative-real-time-polymerase chain reaction (qRT-PCR). Immunohistochemical analyses of three of the encoded proteins suggest that they are expressed in lung vasculature and alveolar regions. The data suggest that the lung alpha-T status may modulate cytoarchitecture of lungs.
Oommen
Saji
S
Division of Pulmonary and Critical Care, Department of Internal Medicine, Genome and Biomedical Sciences Facility, University of California, Davis, CA 95616, USA.
Vasu
Vihas T
VT
Leonard
Scott W
SW
Traber
Maret G
MG
Cross
Carroll E
CE
Gohil
Kishorchandra
K
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D004032:Diet; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D008168:Lung / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D024502:alpha-Tocopherol / Q000378:metabolism / Q000494:pharmacology*
D000975:Antioxidants; D012333:RNA, Messenger; D024502:alpha-Tocopherol