Modulation of the expression of peroxisome proliferators-activated receptors in human fibroblasts.
To determine the levels of peroxisome proliferators-activated receptors (PPARs) in normal and adhesion fibroblasts, we utilized real-time reverse transcription-polymerase chain reaction to measure messenger RNA (mRNA) levels in fibroblasts from normal peritoneum and adhesions from five patients in both the presence or absence of dichloroacetic acid (DCA) and a cyclooxygenase-2 (COX-2) inhibitor, NS-398. Peroxisome proliferators-activated receptor alpha, PPARbeta, PPARgamma1, and PPARgamma2 mRNA are all present in normal peritoneal and adhesion fibroblasts, and selectively rose in response to hypoxia and either DCA or NS-398.
Diamond
Michael P
MP
Saed
Ghassan
G
D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D003999:Dichloroacetic Acid / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D000860:Hypoxia / Q000378:metabolism; D009578:Nitrobenzenes / Q000494:pharmacology*; D047493:PPAR alpha / Q000096:biosynthesis; D047495:PPAR gamma / Q000096:biosynthesis; D048068:PPAR-beta / Q000096:biosynthesis; D010532:Peritoneal Diseases / Q000378:metabolism*; D010537:Peritoneum / Q000187:drug effects / Q000378:metabolism*; D047492:Peroxisome Proliferator-Activated Receptors / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013449:Sulfonamides / Q000494:pharmacology*; D000267:Tissue Adhesions / Q000378:metabolism
D052246:Cyclooxygenase 2 Inhibitors; D009578:Nitrobenzenes; D047493:PPAR alpha; D047495:PPAR gamma; D048068:PPAR-beta; D047492:Peroxisome Proliferator-Activated Receptors; D013449:Sulfonamides; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; D003999:Dichloroacetic Acid