Establishment of retinoblastoma model in NOD-SCID mice and study of metastasis.
OBJECTIVE
To establish model of retinoblastoma subcutaneously in NOD-SCID mice and study rules of formation and distribution of retinoblastoma metastasis.


METHODS
Retinoblastoma cells SO-RB50 were inoculated subcutaneously in NOD-SCID mice. Animal acts and tumor formation, growth and metastasis in NOD-SCID mice were observed. Primary and metastatic tumors were studied pathohistologically by HE and immunohistochemical staining.


RESULTS
The latent periods of tumor growth were 12-19 days and the taken rate of tumor was 100%. 32 days later, 5 NOD-SCID mice were found with tumors that had metastasized to areas mainly located in the abdominal cavity and the side of the kidney; the metastatic time of tumors in the mice also differed. The tumor cells of the primary nodules and the metastasis were similar with human retinoblastoma cells and positive in immunohistochemical staining of NSE.


CONCLUSIONS
The subcutaneous model of retinoblastoma in NOD-SCID mice showed a high taken rate and a short latent period of tumor, which had a high metastatic rate and was the best model in research of behaviors of retinoblastoma at present.
Zhang
Bo
B
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Li
Yongping
Y
Zhong
Xiufeng
X
Huang
Wenge
W
Nie
Li
L
Zhang
Wenxin
W
D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology / Q000556:secondary*; D012175:Retinoblastoma / Q000473:pathology / Q000556:secondary*