title: Purification of hematopoietic stem cells using the side population.
abstract: Hematopoietic stem cells (HSCs) primarily reside in bone marrow, are defined by their ability to maintain blood homeostasis, and replenish themselves through self-renewal. Although HSC purification schemes vary from laboratory to laboratory, the resulting cell populations are similar, if not the same. This chapter will discuss different enrichment methods for HSCs and provide a detailed protocol for staining HSC with Hoechst 33342 for the side population (SP).
authors.affiliation: Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: K K
authors.initials: KK
authors.lastname: Lin
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Goodell
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D000943:Antigens, Differentiation / Q000032:analysis* / Q000096:biosynthesis; D001562:Benzimidazoles; D002469:Cell Separation / Q000379:methods*; D000071063:Endoglin; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000096:biosynthesis; D042787:Receptor, TIE-2 / Q000032:analysis / Q000276:immunology; D011956:Receptors, Cell Surface / Q000032:analysis / Q000276:immunology; D013194:Staining and Labeling
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D001562:Benzimidazoles; C064206:ENG protein, human; D000071063:Endoglin; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D042787:Receptor, TIE-2; C017807:bisbenzimide ethoxide trihydrochloride