Imidazole as a catalyst for in vitro refolding of enhanced green fluorescent protein.
Imidazole is a reagent widely used in protein purifying processes. Here, we reveal a novel chaperone-like activity for imidazole using enhanced green fluorescent protein (EGFP) as a model protein. Experimental results showed that imidazole acted as an effective catalyst for refolding of the chemically denatured EGFP and suppressor for the heat-induced aggregation of EGFP. The refolding kinetics was determined in real time. Both the recovering yield and refolding rate of denatured EGFP in the presence of imidazole were increased. The studies on elucidating the mechanism show that imidazole may catalyze the prolyl cis/trans isomerization and the possible mechanism was discussed. To our knowledge, there are no data on the effect of imidazole on protein folding. Considering the prolyl isomerization is the rate-limited step for refolding of most proteins and aggregation is a universal serious problem for biotechnology, imidazole thus represents a previous unknown type of protein-folding catalyst.
Shi
Ruina
R
College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Pan
Qiong
Q
Guan
Yuan
Y
Hua
Zhendong
Z
Huang
Yong
Y
Zhao
Meiping
M
Li
Yuanzong
Y
D002384:Catalysis; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D011489:Protein Denaturation; D017510:Protein Folding
D007093:Imidazoles; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C029899:imidazole