[Synthesis of xylanolytic enzymes and other carbohydrases by the fungus Penicillium canescens: transformants with an altered copy number of the gene xlnR and an encoding transcriptional activator].
A fragment of Penicillium canescens genomic DNA carrying the xlnR gene coding for a translational activator of xylanolytic genes was isolated. It was demonstrated that a loss of this function in genetically modified transformants resulted in a drastic decrease in the production of P. canescens major xylanases and had a negative effect on the syntheses of several other extracellular xylanases. An increase in the dose of the xlnR gene elevated the xylanolytic activity as well as the activities of a number of other auxiliary enzymes involved in xylan degradation. The activities of two P. canescens major secreted enzymes--beta-galactosidase and alpha-L-arabinofuranosidase-appeared weakly dependent on the translational activator xlnR.
Serebrianyĭ
V A
VA
Sinitsyna
O A
OA
Fedorova
E A
EA
Okunev
O N
ON
Bekkarevich
A O
AO
Sokolova
L M
LM
Vinetskiĭ
Iu P
IuP
Sinitsyn
A P
AP
D000595:Amino Acid Sequence; D001483:Base Sequence; D017353:Gene Deletion; D018628:Gene Dosage; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D016681:Genome, Fungal; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology*; D014170:Transformation, Genetic; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000096:biosynthesis* / Q000235:genetics
D015534:Trans-Activators; D014990:Xylans; D006026:Glycoside Hydrolases; D014995:Xylosidases; C019822:carbohydrase