Abnormal progression through meiosis in men with nonobstructive azoospermia.
OBJECTIVE
To study meiotic abnormalities in men with nonobstructive azoospermia.


METHODS
Analysis of synaptonemal complex and recombination in testicular tissue.


METHODS
Research laboratory.


METHODS
Twenty-nine men with nonobstructive azoospermia and 12 men with normal spermatogenesis.


METHODS
Testicular tissues were processed with immunofluorescent staining using antibodies against proteins associated with synaptonemal complex and recombination events.


METHODS
Synaptonemal complex configuration and recombination in meiosis I.


RESULTS
In patients with nonobstructive azoospermia, a marked heterogeneity in spermatogenesis was found: nearly half of them had a complete absence of meiotic cells, one case had germ cells arrested at the zygotene stage of meiotic prophase, and, in general, the rest had impaired fidelity of chromosome synapsis and recombination in pachytene cells. Compared with controls, these patients had significantly more cells in leptotene/zygotene and higher frequencies of unpaired chromosome regions in pachytene. Significantly reduced recombination, an increased frequency of achiasmate autosome bivalents, and sex univalents in pachytene were also observed in these patients with nonobstructive azoospermia.


CONCLUSIONS
Defects in chromosome synapsis and decreased recombination during meiotic prophase may have led to spermatogenesis arrest and contributed in part to the unexplained infertility in these patients.
Sun
Fei
F
Department of Medical Genetics, University of Calgary, Calgary, Alberta, Canada.
Turek
Paul
P
Greene
Calvin
C
Ko
Evelyn
E
Rademaker
Alfred
A
Martin
Renée H
RH
D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D053713:Azoospermia / Q000503:physiopathology*; D002352:Carrier Proteins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008540:Meiosis / Q000502:physiology*; D000070957:MutL Protein Homolog 1; D009687:Nuclear Proteins / Q000032:analysis; D011995:Recombination, Genetic; D013573:Synaptonemal Complex / Q000502:physiology; D013737:Testis / Q000166:cytology* / Q000503:physiopathology
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C501486:MLH1 protein, human; D009687:Nuclear Proteins; D000070957:MutL Protein Homolog 1