title: Attachment of motile bacterial cells to prealigned holed microarrays.
abstract: Construction of biomotors is an exciting area of scientific research that holds great promise for the development of new technologies with broad potential applications in areas such as the energy industry and medicine. Herein, we demonstrate the fabrication of prealigned microarrays of motile Escherichia coli bacterial cells on SiOx substrates. To prepare these arrays, holed surfaces with a gold layer on the bottom of the holes were utilized. The attachment of bacteria to the holes was achieved via nonspecific interactions using poly-l-lysine hydrobromide (PLL). Our data suggest that a single motile bacterial cell can be selectively attached to an individual hole on a surface and bacterial cell binding can be controlled by altering the pH, with the greatest occupancy occurring at pH 7.8. Cells attached to hole arrays remained motile for at least 4 h. These data indicate that holed surface structures provide a promising footprint for the attachment of motile bacterial cells to form high-density site-specific functional bacterial microarrays.
authors.affiliation: The Department of Chemistry, Loyola University-Chicago, 1068 N. Sheridan Road, Chicago, IL 60626, USA.
authors.forename: Sergey
authors.initials: S
authors.lastname: Rozhok
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Fan
authors.forename: Dorjderem
authors.initials: D
authors.lastname: Nyamjav
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Chad A
authors.initials: CA
authors.lastname: Mirkin
authors.forename: Richard C
authors.initials: RC
authors.lastname: Holz
mesh_terms: D018914:Cells, Immobilized / Q000737:chemistry / Q000166:cytology; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology*; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D046228:Microarray Analysis / Q000379:methods; D011107:Polylysine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011107:Polylysine; D006046:Gold; D012822:Silicon Dioxide