title: Osteoblastic cell response on fluoridated hydroxyapatite coatings.
abstract: Fluoridated hydroxyapatite (FHA) coatings were deposited onto Ti6Al4V substrates by sol-gel dip-coating method. X-ray photoelectron spectroscopy results showed that fluoride ions were successfully incorporated into the hydroxyapatite (HA) lattice structure. The dissolution behavior in Tris-buffered physiological saline indicated that all fluoridated HA coatings had lower solubility than that of the pure HA coating. The lowest solubility was obtained at fluoride ion concentrations of 0.8-1.1M. In vitro cell responses were evaluated with human osteosarcoma MG63 cells in terms of cell morphology, proliferation and differentiation (alkaline phosphatase activity and osteocalcin level). For all coatings tested, similar cell morphologies and good cell viability were observed. Coatings fluoridated to 0.8-1.1 had a stronger stimulating effect on cell proliferation and differentiation activities. The influences on cell phenotypes were attributed mainly to a combined ion effect of Ca, P and F released from the coating during dissolution. For the best dissolution resistance and cell activities, it is recommended that the molar level of fluoride ion be from 0.8 to 1.1, such that the coating takes the form of Ca(10)(PO(4))(6)(OH)(1.2-0.9)F(0.8-1.1).
authors.affiliation: School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Sam
authors.initials: S
authors.lastname: Zhang
authors.forename: Xianting
authors.initials: X
authors.lastname: Zeng
authors.forename: Lwin Lwin
authors.initials: LL
authors.lastname: Ma
authors.forename: Wenjian
authors.initials: W
authors.lastname: Weng
authors.forename: Weiqi
authors.initials: W
authors.lastname: Yan
authors.forename: Min
authors.initials: M
authors.lastname: Qian
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D005461:Fluorine / Q000737:chemistry*; D006801:Humans; D007477:Ions; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology*; D012516:Osteosarcoma / Q000378:metabolism; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D007477:Ions; D005461:Fluorine; D017886:Durapatite; D000469:Alkaline Phosphatase