Role of glutamate in the rostral ventrolateral medulla in acupuncture-related modulation of visceral reflex sympathoexcitation.
Visceral sympathoexcitatory reflexes induced by stimulation of the gallbladder with bradykinin (BK) are attenuated by electroacupuncture (EA) at Neiguan-Jianshi (P5-6) acupoints located over the median nerve. Previous studies have shown that neurons in the rostral ventrolateral medulla (rVLM) receive convergent input from visceral organs and somatic nerves (activated by EA). Glutamate (Glu), an important excitatory neurotransmitter in the rVLM, processes visceral sympathoexcitatory cardiovascular reflexes. In the present study, we determined the relation between EA-mediated opioidergic modulation of visceral cardiovascular responses and Glu. Reflex cardiovascular responses were evoked by application of BK to the gallbladder before and after EA in anesthetized cats. Glu concentrations ([Glu]) were measured by HPLC from samples collected by microdialysis probe(s) inserted unilaterally or bilaterally into the rVLM. BK-induced reflex responses and [Glu] were attenuated by 45% and 70%, respectively, after 30 min of EA (n = 6). EA alone did not change [Glu] in the rVLM (n = 6, P > 0.05). However, microdialysis of naloxone (100 mM) into the rVLM reversed EA-related inhibition of blood pressure and [Glu] (n = 5). Immunohistochemical visualization showed that delta-opioid receptors colocalized with, and were in close apposition to, vesicular Glu transporter 3- and c-Fos-double-labeled perikarya and processes of rVLM neurons after gallbladder stimulation with BK. These data suggest that EA attenuates BK-induced visceral sympathoexcitatory reflexes through opioid-mediated inhibition of Glu's action in the rVLM.
Zhou
Wei
W
Department of Medicine and Susan Samueli Center for Integrative Medicine, College of Medicine, University of California, Irvine, California 92697-4075, USA. wzhou2@uci.edu
Fu
Liang-Wu
LW
Guo
Zhi-Ling
ZL
Longhurst
John C
JC
D026881:Acupuncture; D000818:Animals; D002415:Cats; D005260:Female; D018698:Glutamic Acid / Q000494:pharmacology / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008526:Medulla Oblongata / Q000502:physiology*; D017551:Microdialysis; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D017465:Receptors, Opioid, delta / Q000378:metabolism; D012018:Reflex / Q000502:physiology*; D013268:Stimulation, Chemical; D013564:Sympathetic Nervous System / Q000502:physiology*; D050496:Vesicular Glutamate Transport Proteins / Q000378:metabolism; D017833:Visceral Afferents / Q000502:physiology
D009292:Narcotic Antagonists; D016760:Proto-Oncogene Proteins c-fos; D017465:Receptors, Opioid, delta; D050496:Vesicular Glutamate Transport Proteins; D009270:Naloxone; D018698:Glutamic Acid