Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening.
White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease.
Yuan
Li
L
Institute of Hydrobiology, Chinese Academy of Sciences, 7 East Lake Southern Road, Wuhan 430072, PR China.
Zhang
Xiaohua
X
Xiao
Nan
N
Dai
Lingfen
L
Chen
Weizhou
W
Jia
Chensong
C
Zhao
Ruohong
R
Hemmingsen
Sean M
SM
Dai
Heping
H
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D001483:Base Sequence; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006458:Hemolymph / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000166:cytology / Q000821:virology*; D019151:Peptide Library; D016415:Sequence Alignment; D013552:Swine; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000648:ultrastructure
D000914:Antibodies, Viral; D007106:Immune Sera; D019151:Peptide Library