title: Gene manipulation using artificial restriction DNA cutter.
abstract: Artificial restriction DNA cutter (ARCUT), which we developed recently, was used for manipulation of plasmid DNA. PCR product was inserted into pBR322 plasmid vector using ARCUT, and E. coli cells were transfected with this recombinant plasmid DNA. Successful growth of cells shows that the recombination proceeds without any unexpected mutations. Furthermore, this artificial system was applied to PCR-free construction of chimera protein.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Yoji
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Jing-Min
authors.initials: JM
authors.lastname: Zhou
authors.forename: Takafumi
authors.initials: T
authors.lastname: Tomita
authors.forename: Akihiko
authors.initials: A
authors.lastname: Uehara
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Aiba
authors.forename: Akira
authors.initials: A
authors.lastname: Watanabe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Aburatani
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D002563:Cerium / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006868:Hydrolysis; D020135:Peptide Nucleic Acids / Q000737:chemistry; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D011995:Recombination, Genetic; D014162:Transfection
chemical_list: D020135:Peptide Nucleic Acids; D011993:Recombinant Fusion Proteins; D002563:Cerium; D004247:DNA