Simultaneous profiling of N-glycans and proteins from human serum using a parallel-column system directly coupled to mass spectrometry.
A method for the rapid identification of proteins and their N-glycans was developed through the use of two parallel columns directly connected to a mass spectrometer. Both porous graphitic carbon (PGC) and C18 capillary columns were connected in parallel with two switching valves for the simultaneous analysis of glycans and peptides, respectively. To verify the efficiency of the analytical system, profiling of N-glycans and proteins from human serum was demonstrated. This method is suitable for high-throughput analysis and automation, is contamination-free for the identification of N-glycans and proteins in a complex biological sample, and can be applied to glycomics and proteomics.
Kim
Yun-Gon
YG
Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, Seoul, Republic of Korea.
Jang
Kyoung-Soon
KS
Joo
Hwang-Soo
HS
Kim
Hyun-Ki
HK
Lee
Chang-Soo
CS
Kim
Byung-Gee
BG
D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006801:Humans; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
D001798:Blood Proteins; D011134:Polysaccharides