Developmental expression of transferrin binding protein in oligodendrocyte lineage cells of the embryonic chick spinal cord.
Oligodendrocytes develop from precursor cells in the neuroepithelium of the ventral ventricular zone. Oligodendrocytes in the different stages of development are characterized by expression of a number of different marker molecules such as myelin genes, growth factors, and specific antigens. We have previously identified that transferrin binding protein (TfBP), a member of heat shock protein 90 families, is a novel avian ER-associated membrane protein that is specifically localized in oligodendrocytes in adult chicken CNS. In this study we describe the developmental expression of TfBP in the embryonic chick spinal cord. A few, distinct, TfBP+ cells appeared at the lateral margin of the subventricular neuroepithelium of the spinal cord at E7. Thereafter, some TfBP+ cells, exhibited a migrative form of unipolar or bipolar shape occurred around E8 in the mantle layer, midway between the neuroepithelium and the marginal layer of the primitive spinal cord. Thereafter, the TfBP+ cells rapidly increased in number as well as their staining intensity, and overall distribution of TfBP+ cells at E15 was comparable to that of a mature spinal cord. Our observations suggest that TfBP is expressed in the subpopulation of oligodendrocyte lineage in the development and a putative role of TfBP in relation to transferrin and iron trafficking is considered.
Park
Sang Wook
SW
Department of Anatomy, Seoul National University College of Medicine, 28, Yongon-Dong, Chongno-Gu, Seoul 110-799, Korea.
Lim
Ha Seong
HS
Roh
Eun Jung
EJ
Kim
Dong Woon
DW
Jeon
Gye Sun
GS
Cho
Sa Sun
SS
D000818:Animals; D015153:Blotting, Western; D019070:Cell Lineage; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009836:Oligodendroglia / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D013116:Spinal Cord / Q000196:embryology; D033863:Transferrin-Binding Proteins / Q000096:biosynthesis*
D033863:Transferrin-Binding Proteins