Formation of novel double-stranded structures formed by oligodeoxyribonucleotides carrying aromatic groups.
A novel thymidine-methyl red conjugate was synthesized and incorporated into oligodeoxyribonucleotides (ODNs). Each thermal denaturation profile of solutions of the ODNs showed a sharp transition curves. The melting temperatures corresponding to the transition curves became higher as the salt concentrations of the solutions increased. These results may indicate the formation of duplex structures in which two strands are connected via the stacking interactions of aromatic residues that are attached to DNA strands.
Ikeya
Satoshi
S
Department of Chemistry, Graduate School and Faculty of Science, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachioji, Tokyo 192-0397, Japan.
Ono
Akira
A
D001391:Azo Compounds / Q000138:chemical synthesis; D002942:Circular Dichroism; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013936:Thymidine / Q000138:chemical synthesis / Q000737:chemistry
D001391:Azo Compounds; D009838:Oligodeoxyribonucleotides; C008492:methyl red; D013936:Thymidine