title: Susceptibility of Streptococcus thermophilus to antibiotics.
abstract: A total of 74 Streptococcus thermophilus isolates collected between 1948 and 2005 from different environments were investigated to assess erythromycin, clindamycin, streptomycin, gentamicin, tetracycline and ampicillin susceptibility by means of microdilution, Etest and disk diffusion methods. For this purpose a new S. thermophilus Susceptibility test Medium (SSM) was developed. This medium allowed a better identification of strains with atypical tetracycline resistance. The recipe is a mixed formulation of Iso-Sensitest medium (90% v/v) and M17 medium (10% v/v) supplemented with lactose (0.5% w/v). The overall agreement of the techniques was good with exception of tetracycline, for which Etest provided lower MICs than the microdilution method. Most strains were susceptible to all the antibiotics tested while a few erythromycin, tetracycline and streptomycin resistant strains were detected.
authors.affiliation: Institute of Microbiology, Università Cattolica del Sacro Cuore, via Emilia Parmense 84, 29100, Piacenza, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Tosi
authors.forename: Giangiacomo
authors.initials: G
authors.lastname: Berruti
authors.forename: Morten
authors.initials: M
authors.lastname: Danielsen
authors.forename: Anette
authors.initials: A
authors.lastname: Wind
authors.forename: Geert
authors.initials: G
authors.lastname: Huys
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Morelli
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002981:Clindamycin / Q000494:pharmacology; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D004917:Erythromycin / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D048249:Streptococcus thermophilus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D013307:Streptomycin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D013754:Tetracyclines / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D005839:Gentamicins; D013754:Tetracyclines; D002981:Clindamycin; D004917:Erythromycin; D000667:Ampicillin; D004262:DNA Restriction Enzymes; D013752:Tetracycline; D013307:Streptomycin