Hematopoietic stem cells.
Hematopoietic stem cells (HSCs) have the capacity to self-renew and the potential to differentiate into all of the mature blood cell types. The ability to prospectively identify and isolate HSCs has been the subject of extensive investigation since the first transplantation studies implying their existence almost 50 years ago. Despite significant advances in enrichment protocols, the continuous in vitro propagation of human HSCs has not yet been achieved. This chapter describes current procedures used to phenotypically and functionally characterize candidate human HSCs and initial efforts to derive permanent human HSC lines.
Hawley
Robert G
RG
Department of Anatomy and Cell Biology, The George Washington University Medical Center, Washington, DC, USA.
Ramezani
Ali
A
Hawley
Teresa S
TS
D000818:Animals; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans