Methylation status of imprinting centers for H19/IGF2 and SNURF/SNRPN in primate embryonic stem cells.
Embryonic stem cells (ESCs) hold promise for cell and tissue replacement approaches to treating human diseases based on their capacity to differentiate into a wide variety of somatic cells and tissues. However, long-term in vitro culture and manipulations of ESCs may adversely affect their epigenetic integrity, including imprinting. We have recently reported aberrant biallelic expression of IGF2 and H19 in several rhesus monkey ESC lines, whereas SNRPN and NDN were normally imprinted and expressed predominantly from the paternal allele. The dysregulation of IGF2 and H19 that is associated with tumorigenesis in humans may result from improper maintenance of allele-specific methylation patterns at an imprinting center (IC) upstream of H19. To test this possibility, we performed methylation analysis of several monkey ESC lines by genomic bisulfite sequencing. We investigated methylation profiles of CpG islands within the IGF2/H19 IC harboring the CTCF-6 binding site. In addition, the methylation status of the IC within the promoter/exon 1 of SNURF/SNRPN known as the Prader-Willi syndrome IC was examined. Our results demonstrate abnormal hypermethylation within the IGF2/H19 IC in all analyzed ESC lines, whereas the SNURF/SNRPN IC was differentially methylated, consistent with monoallelic expression.
Mitalipov
Shoukhrat
S
Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health & Science University, 505 NW 185th Ave., Beaverton, OR 97006, USA. mitalipo@ohsu.edu
Clepper
Lisa
L
Sritanaudomchai
Hathaitip
H
Fujimoto
Akihisa
A
Wolf
Don
D
D000818:Animals; D001324:Autoantigens / Q000235:genetics*; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D019175:DNA Methylation; D053595:Embryonic Stem Cells / Q000502:physiology*; D005786:Gene Expression Regulation; D018392:Genomic Imprinting; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008253:Macaca mulatta; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics*; D055517:snRNP Core Proteins
D001324:Autoantigens; C094335:H19 long non-coding RNA; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D017411:Ribonucleoproteins, Small Nuclear; C526417:SNRPN protein, human; D055517:snRNP Core Proteins; D007335:Insulin-Like Growth Factor II; D004247:DNA