title: Conformational change of a nucleotide by a base-pair mimic nucleoside in the complementary DNA strand.
abstract: On the basis of the non-covalent bond interactions in nucleic acids, we have synthesized a novel adenosine derivative tethering a phenyl group. Structure and thermal stability of the DNA duplexes bearing the adenosine derivative in the middle of a strand were investigated by CD spectra and the UV melting curves. We found that the conformation and the interactions of the duplexes were similar regardless of the nucleotide facing with the adenosine derivative in a duplex. This observation suggests the base pair-mimic geometry of the adenosine derivative accompanied with a conformational change of the nucleotide in the complementary strand.
authors.affiliation: Frontier Institute for Biomolecular Engineering Research, Konan University, Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
authors.forename: Shu-ichi
authors.initials: S
authors.lastname: Nakano
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Uotani
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Anno
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fujii
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugimoto
mesh_terms: D000241:Adenosine / Q000737:chemistry*; D020029:Base Pairing; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry*; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes; D009711:Nucleotides / Q000737:chemistry*
chemical_list: D018076:DNA, Complementary; D009692:Nucleic Acid Heteroduplexes; D009711:Nucleotides; D000241:Adenosine