Structural analysis of DNA containing (6-4) photoproduct in complex with distamycin A by NMR.
We reported that distamycin A can bind to DNA containing the (6-4) photoproduct, one of the major UV lesions in DNA, by means of CD spectroscopy. Here, we have analyzed the structure of DNA containing the (6-4) photoproduct in complex with distamycin A by NMR. Broadening of NMR resonances and large chemical shift perturbation have been observed for residues located close to the (6-4) photoproduct in DNA, indicating the binding to this region. Intermolecular NOEs between DNA and distamycin A have been identified for some of these residues, suggesting the formation of the relatively tight complex. On the basis of these NOEs, structure determination of the complex is now in progress.
Tsuchibayashi
Hiroaki
H
Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29 Suehirocho, Tsurumi, Yokohama, Kanagawa 230-0045, Japan.
Ohyama
Takako
T
Matsugami
Akimasa
A
Nanjo
Takehiro
T
Iwai
Shigenori
S
Katahira
Masato
M
D001665:Binding Sites; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D004214:Distamycins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011740:Pyrimidine Dimers / Q000737:chemistry*
D004214:Distamycins; D011740:Pyrimidine Dimers; C058406:pyrimidine-pyrimidone dimer; C030000:stallimycin; D004247:DNA