title: Disruption of ins-11, a Caenorhabditis elegans insulin-like gene, and phenotypic analyses of the gene-disrupted animal.
abstract: The insulin/insulin-like growth factor-I signaling (IIS) pathway regulates larval diapause, adult lifespan, fat metabolism, and stress-resistance in the nematode Caenorhabditis elegans. One of 38 C. elegans insulin-like genes, ins-11, was disrupted and phenotypic analyses of the gene-disrupted animal were performed. The gene-disruption exhibited a significant influence on the adult lifespan. It antagonized the lifespan extension induced by RNAi knockdown of another insulin-like gene, ins-7. Hence ins-11 appears to be necessary for lifespan extension caused by a decrease in the IIS pathway. This is the first description of gene-disruption of the C. elegans insulin-like gene that suppresses the lifespan extension.
authors.affiliation: Department of Biological and Environmental Chemistry, Faculty of Agriculture, Tottori University. kawano@muses.tottori-u.ac.jp
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kawano
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Nagatomo
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kimura
authors.forename: Keiko
authors.initials: K
authors.lastname: Gengyo-Ando
authors.forename: Shohei
authors.initials: S
authors.lastname: Mitani
mesh_terms: D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary; D007328:Insulin / Q000235:genetics*; D010641:Phenotype; D034622:RNA Interference
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D007328:Insulin