Molecular labeling of the CGG trinucleotide repeat.
The new molecular ligand naphthyridine carbamate dimer (NC), possessing 2-amino-1,8-naphthyridines and a carbamate linker, specially binds to guanine-guanine (G-G) mismatch in duplex DNA. The results of T(m) measurements showed that NC selectively bound to CGG/CGG triad with high deltaT(m) of 23.1 degrees C. The exclusive stoichiometry 2:1 of the complex of NC with CGG/CGG triad, obtained by the measurements of cold spray ionization time-of-flight mass spectrometry (CSI-TOF MS), showed that NC bound to CGG/CGG triad strongly with two molecules.
Peng
Tao
T
The Institute of Scientific and Industrial Research, Osaka University, Ibaraki 567-0047, Japan.
Nakatani
Kazuhiko
K
D020137:Base Pair Mismatch; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D009287:Naphthyridines / Q000138:chemical synthesis / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D018911:Trinucleotide Repeats
D015335:Molecular Probes; D009287:Naphthyridines; C520186:bis((7-methylnaphthyridin-2-yl)carbamoylpropyl)amine