Comparative protein expression profiling in human cumulus cells in relation to oocyte fertilization and ovarian stimulation protocol.
Comparative profiling was performed on proteins synthesized in human cumulus cells (CC) from individual oocytes recovered after two different ovarian stimulation protocols for classical IVF (cIVF). Using high-resolution two-dimensional protein electrophoresis after metabolic labelling with [35S]-methionine, protein expression was profiled in CC of metaphase II oocytes obtained after two different ovarian stimulation protocols (rFSH versus human menopausal gonadotrophin). Analysis was done on CC from two cIVF cycles in the same patient and then extended to CC from individual oocytes from two groups of patients. CC from single oocytes have robust levels of protein expression into 600-800 protein spots. Comparison of CC protein expression from oocytes obtained from the same patient but after two different stimulation protocols shows that the type of hormonal treatment influences CC protein expression. In contrast, CC from oocytes obtained under the same stimulation protocol but with different fertilization outcome show a high profile similarity with differences in only a few spots. Comparison of two groups of patients indicates that dissimilarities in protein pattern between patients become very high, even when comparing the same stimulation protocol and oocyte fertilization outcome. Thus protein expression profiling of human CC may provide a correlation between the synthesis of specific cumulus proteins and maturity and fecundity.
Hamamah
Samir
S
Service de Biologie de la Reproduction, Hôpital Arnaud de Villeneuve, Montpellier Cedex 5, France. s-hamamah@chu-montpellier.fr
Matha
Valérie
V
Berthenet
Cyril
C
Anahory
Tal
T
Loup
Vanessa
V
Dechaud
Hervé
H
Hedon
Bernard
B
Fernandez
Anne
A
Lamb
Ned
N
D005260:Female; D005307:Fertilization in Vitro; D043373:Follicle Stimulating Hormone, Human / Q000008:administration & dosage; D006801:Humans; D008297:Male; D008596:Menotropins / Q000008:administration & dosage; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D010062:Ovulation Induction / Q000379:methods; D040081:Protein Array Analysis; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics; D011994:Recombinant Proteins / Q000008:administration & dosage
D043373:Follicle Stimulating Hormone, Human; D011506:Proteins; D011994:Recombinant Proteins; D008596:Menotropins