title: Evaluation and modification of N-trimethyl chitosan chloride nanoparticles as protein carriers.
abstract: N-Trimethyl chitosan chloride (TMC) nanoparticles were prepared by ionic crosslinking of TMC with tripolyphosphate (TPP). Two model proteins with different pI values, bovine serum albumin (BSA, pI=4.8) and bovine hemoglobin (BHb, pI=6.8), were used to investigate the loading and release features of the TMC nanoparticles. TMC samples with different degrees of quaternization were synthesized to evaluate its influence on the physicochemical properties and release profiles of the nanoparticles. Sodium alginate was used to modify the TMC nanoparticles to reduce burst release. The results indicated that the TMC nanoparticles had a high loading efficiency (95%) for BSA but a low one (30%) for BHb. The particle size and zeta potential were significantly affected by the BSA concentration but not by the BHb concentration. Nanoparticles of TMC with a lower degree of quaternization showed an increase in particle size, a decrease in zeta potential and a slower drug-release profile. As for the alginate-modified nanoparticles, a smaller size and lower zeta potential were observed and the burst release of BSA was reduced. These studies demonstrated that TMC nanoparticles are potential protein carriers, and that their physicochemical properties and release profile could be optimized by means of various modifications.
authors.affiliation: Key Laboratory of Drug Targeting of Ministry of Education, West China School of Pharmacy, Sichuan University, No. 17, Block 3, Southern Renmin Road, Chengdu 610041, PR China.
authors.forename: Fu
authors.initials: F
authors.lastname: Chen
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D002417:Cattle; D011232:Chemical Precipitation; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry / Q000493:pharmacokinetics; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D011122:Polyphosphates / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000493:pharmacokinetics; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000493:pharmacokinetics; D055672:Static Electricity
chemical_list: D000464:Alginates; D003102:Colloids; D004337:Drug Carriers; D006454:Hemoglobins; D006603:Hexuronic Acids; C118071:N-trimethyl chitosan chloride; D011122:Polyphosphates; D011506:Proteins; D012710:Serum Albumin, Bovine; D020723:Glucuronic Acid; D048271:Chitosan; C005692:triphosphoric acid