title: Comparison study on PCR amplification of modified DNA by using various kinds of polymerase and modified nucleoside triphosphates.
abstract: A series of C5 substituted analogs of 2'-deoxyuridine triphosphate and 2'-deoxycytidine triphosphate were synthesized and investigated their substrate properties for thermostable DNA polymerases during polymerase chain reaction (PCR). The PCR assays indicated that the relative yield of the full-length product is dependent on whether the substitution is on dU or dC, and on the nature of the polymerase.
authors.affiliation: Department of Applied Chemistry, Faculty of Engneering, Gunma University, 1-5-1 Tenjin-chou, Kiryu, Gunma 376-8515, Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Takehiro
authors.initials: T
authors.lastname: Tamura
authors.forename: Rina
authors.initials: R
authors.lastname: Kitagata
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sawai
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ozaki
mesh_terms: D004247:DNA / Q000096:biosynthesis / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003845:Deoxycytosine Nucleotides / Q000737:chemistry / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides / Q000737:chemistry / Q000378:metabolism*; D016133:Polymerase Chain Reaction
chemical_list: D003845:Deoxycytosine Nucleotides; D003856:Deoxyuracil Nucleotides; C027078:deoxyuridine triphosphate; C024107:2'-deoxycytidine 5'-triphosphate; D004247:DNA; D004259:DNA-Directed DNA Polymerase