title: Regulation of intestinal immunity: effects of the oral adjuvant Escherichia coli heat-labile enterotoxin on migrating dendritic cells.
abstract: Escherichia coli heat-labile enterotoxin (Etx) is an oral adjuvant in mice. We show that this is also true for rats. To understand this adjuvant activity we examined lymph dendritic cells (DC) migrating from the intestine to mesenteric lymph nodes (MLN) in animals fed Etx. These DC can prime antigen-specific antibody responses. We show that in rats the small intestine contains 7-24 million DC and 8 x 10(5 )of these migrate to MLN each day. Surprisingly, Etx does not stimulate increased migration of lymph DC. However, oral Etx affects the activation, antigen transport and localization of migratory DC. Specifically, expression of CD25 increases on the CD172a(high) subset of lymph DC. Oral Etx also increases the number of CD172a(high) lymph DC containing co-administered ovalbumin. CD172a(high) lymph DC treated with Etx in vitro, or purified from the lymph of animals fed Etx, stimulate stronger proliferative responses from primed T cells. Etx also directs more of the CD172a(high) lymph DC into the central region of the MLN T cell areas. This change in DC localization is associated with an increase in the expression of CCR7. These data help advance our understanding of the role of DC in initiating mucosal immune responses in vivo.
authors.affiliation: Sir William Dunn School of Pathology, Oxford, UK. simon.milling@path.ox.ac.uk
authors.forename: Simon W F
authors.initials: SW
authors.lastname: Milling
authors.forename: Ulf
authors.initials: U
authors.lastname: Yrlid
authors.forename: Chris
authors.initials: C
authors.lastname: Jenkins
authors.forename: Claire M
authors.initials: CM
authors.lastname: Richards
authors.forename: Neil A
authors.initials: NA
authors.lastname: Williams
authors.forename: Gordon
authors.initials: G
authors.lastname: MacPherson
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000284:Administration, Oral; D000818:Animals; D001427:Bacterial Toxins / Q000008:administration & dosage* / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D004768:Enterotoxins / Q000008:administration & dosage* / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000008:administration & dosage* / Q000276:immunology*; D007108:Immune Tolerance; D018928:Immunity, Mucosal; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008643:Mesentery; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli