title: Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element.
abstract: The technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we were able to successfully transform Aedes fluviatilis (Lutz), which can be used as an avian malaria model. These results demonstrate the usefulness of the piggyBac transposable element as a transformation vector for Neotropical mosquito species and opens up new research frontiers for South American mosquito vectors.
authors.affiliation: Laboratório de Malária, Centro de Pesquisas René Rachou, Av. Augusto de Lima 1715, Fiocruz, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Flávia Guimarães
authors.initials: FG
authors.lastname: Rodrigues
authors.forename: Sabrina Barbosa
authors.initials: SB
authors.lastname: Oliveira
authors.forename: Bruno Coelho
authors.initials: BC
authors.lastname: Rocha
authors.forename: Luciano Andrade
authors.initials: LA
authors.lastname: Moreira
mesh_terms: D000330:Aedes / Q000235:genetics*; D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D005260:Female; D018014:Gene Transfer Techniques; D017344:Genes, Insect; D005854:Germ Cells; D007303:Insect Vectors / Q000235:genetics*; D008297:Male; D008845:Microinjections; D014170:Transformation, Genetic / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements