Structure of RNA aptamer complexed with an RNA-binding peptide of Tat with aid of residue-specific 13C, 15N labeling.
An RNA aptamer containing two binding sites of HIV Tat exhibits extremely high affinity to Tat. We have determined the structure of the aptamer complexed with an RNA-binding peptide of Tat. The analysis was made feasible by the use of several peptides in which a single arginine residue was specifically 13C, 15N-labeled. Residue specific labeling of the peptide enhanced the identification of intermolecular contacts, which are otherwise hard to identify due to spectral overlapping. The structure of the complex has revealed the origin of the high affinity of the aptamer to Tat.
Matsugami
Akimasa
A
Supramolecular Biology, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Tochio
Hidehito
H
Niyada
Emi
E
Tamura
Yusuke
Y
Kudo
Michiko
M
Misono
Tomoko S
TS
Kumar
Penmetcha
P
Katahira
Masato
M
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001120:Arginine / Q000737:chemistry; D001665:Binding Sites; D002247:Carbon Isotopes; D015696:Gene Products, tat / Q000737:chemistry* / Q000378:metabolism; D006678:HIV; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D054322:tat Gene Products, Human Immunodeficiency Virus
D052157:Aptamers, Nucleotide; D002247:Carbon Isotopes; D015696:Gene Products, tat; D009587:Nitrogen Isotopes; D010455:Peptides; D016601:RNA-Binding Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D001120:Arginine