title: Construction of a stable expression vector for Leifsonia xyli subsp. cynodontis and its application in studying the effect of the bacterium as an endophytic bacterium in rice.
abstract: To study the possibility of utilizing genetically engineered Leifsonia xyli subsp. cynodontis (Lxc) as an endophytic bacterium in rice, we constructed an Escherichia coli-Lxc shuttle vector, pLGUS, containing a beta-glucuronidase reporter gene, which was stable both in vitro and in vivo. Lxc grows and expresses the beta-glucuronidase reporter gene in all parts of rice, except for seed. A 2-year field study using three rice varieties from China showed that Lxc inoculation did not have a negative effect on the growth and yield of any of these varieties. Therefore, Lxc has the potential to be used as a benign endophyte for the expression of foreign genes in rice.
authors.affiliation: Department of Biotechnology, College of Life Sciences, Wuhan University, Wuhan, Hubei, China.
authors.forename: Tai-Yuan
authors.initials: TY
authors.lastname: Li
authors.forename: Han-Lai
authors.initials: HL
authors.lastname: Zeng
authors.forename: Yin
authors.initials: Y
authors.lastname: Ping
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
authors.forename: Xue-Lian
authors.initials: XL
authors.lastname: Fan
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Guo
authors.forename: Chu-Fu
authors.initials: CF
authors.lastname: Zhang
mesh_terms: D000192:Actinomycetales / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000032:analysis; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics
chemical_list: D005966:Glucuronidase