title: The protective effects of phenolic constituents from Gastrodia elata on the cytotoxicity induced by KCl and glutamate.
abstract: Seven phenolic compounds (1-7) were isolated from the tubers of Gastrodia elata. Their structures were elucidated on the basis of MS and NMR spectral data. p-Ethoxymethyl phenyl-O-beta-D-glucoside (1) was proved to be a new compound, with N-(p-hydroxybenzyl)-adenosine (7) isolated from this plant for the first time. In this study, the protective effects of the six constituents (1-6) on PC12 cells against the cytotoxicity induced by KCl and glutamate were also investigated. The viability of the PC12 cells was significantly enhanced by pretreatment with the six phenolic constituents.
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Zhan-Bo
authors.initials: ZB
authors.lastname: Huang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wu
authors.forename: Fa-Kui
authors.initials: FK
authors.lastname: Chen
authors.forename: Li-Bo
authors.initials: LB
authors.lastname: Zou
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001593:Benzyl Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D005019:Ethyl Ethers / Q000302:isolation & purification / Q000494:pharmacology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D031668:Gastrodia / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D018698:Glutamic Acid / Q000633:toxicity*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology; D016716:PC12 Cells; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D035281:Plant Tubers / Q000737:chemistry; D011189:Potassium Chloride / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D051381:Rats; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001593:Benzyl Compounds; D005019:Ethyl Ethers; D018691:Excitatory Amino Acid Antagonists; D005960:Glucosides; C515642:N-(p-hydroxybenzyl)-adenosine; D018696:Neuroprotective Agents; D010636:Phenols; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; C515641:p-ethoxymethyl phenyl-O-beta-D-glucoside; D018698:Glutamic Acid; D011189:Potassium Chloride; C022616:thiazolyl blue; D000241:Adenosine