title: Crystallization and preliminary X-ray analysis of RNA oligomers containing CUG repeats which induce type 1 myotonic dystrophy.
abstract: To reveal specific structure of CUG tandem repeats, which induce type 1 myotonic dystrophy, several fragments with different number of repeats have been synthesized, purified and crystallized. Their native PAGEs show that the structural stabilities of (CUG)6 and (CUG)7 are different, suggesting that when they adopt a hairpin form, the loops have different structures depending on the even and odd numbers of repeats. A preliminary structure of (CUG)6 crystal shows that the fragment forms a duplex similar to an A-form RNA.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Sato
authors.forename: Kousei
authors.initials: K
authors.lastname: Kimura
authors.forename: Akio
authors.initials: A
authors.lastname: Takénaka
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D009223:Myotonic Dystrophy / Q000235:genetics*; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D012313:RNA / Q000737:chemistry* / Q000235:genetics*; D055672:Static Electricity; D019680:Trinucleotide Repeat Expansion
chemical_list: D009692:Nucleic Acid Heteroduplexes; D012313:RNA