title: NMR spectroscopic analyses of functional nucleic acids-metal interaction and their solution structure analyses.
abstract: We have studied complexations of functional nucleic acids and metal ions, by means of NMR spectroscopy. In the case of hammerhead ribozymes, they have a metal ion-binding motif in their core sequences. Upon the metallation of N7 of a guanosine in an RNA duplex modelled after hammerhead ribozymes, 20 ppm higher field shift of N7 was observed in 1-dimensional (1D) 15N NMR spectra. It was found that metal ion binding to nucleobases were detectable with 15N NMR spectroscopy.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Shuji
authors.initials: S
authors.lastname: Oda
authors.forename: Chojiro
authors.initials: C
authors.lastname: Kojima
authors.forename: Akira
authors.initials: A
authors.lastname: Ono
authors.forename: Kazunari
authors.initials: K
authors.lastname: Taira
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kondo
mesh_terms: D001665:Binding Sites; D008670:Metals / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009843:Oligoribonucleotides / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry*; D012996:Solutions
chemical_list: D008670:Metals; D009843:Oligoribonucleotides; D016337:RNA, Catalytic; D012996:Solutions; C427204:hammerhead ribozyme