Extraction and quantification of gymnemic acids through gymnemagenin from callus cultures of Gymnema sylvestre.
The phyto-constituents of Gymnema sylvestre are used in the treatment of diabetes and obesity. The present work reports on the extraction of gymnemic acid through gymnemagenin from callus cultures of G. sylvestre. Components were separated on pre-coated silica gel 60 GF254 plates with chloroform:methanol (8:2) and scanned using a densitometric scanner at 205 nm in the near-UV region. Linearity of determination of gymnemagenin was observed in the range 2-10 microg. The average percentage recovery of gymnemagenin from leaf callus extracts was 98.9+/-0.3.
Kanetkar
P V
PV
Food Engineering and Technology Department, Institute of Chemical Technology (ICT), University of Mumbai, Matungoa, Mumbai 400 019, Maharashtra, India.
Singhal
R S
RS
Laddha
K S
KS
Kamat
M Y
MY
D000470:Alkaloids / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D031104:Gymnema sylvestre / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012503:Saponins / Q000032:analysis*; D012639:Seeds / Q000737:chemistry; D046509:Tissue Culture Techniques; D014315:Triterpenes / Q000032:analysis*
D000470:Alkaloids; D012503:Saponins; D014315:Triterpenes; C007898:gymnemic acid; C517988:gymnemagenin