[Isolation and characterization of Hantavirus carried by rodents in Huludao, Liaoning province].
OBJECTIVE
To investigate the Hantavirus infection and their genotype in rodents in Huludao.


METHODS
Rodents were collected from the main epidemic areas to detect antigen of Hantavirus in rat lungs by indirect immunofluorescence assay. Antigen-positive samples were inoculated onto cultures of confluent Vero E6 cells for the isolation of virus. The genotypes of viruses in all antigen-positive samples were identified by reverse transcriptase-polymerase chain reaction (RT-PCR).


RESULTS
200 rats were collected in the main epidemic areas, and 11 Hantavirus-positive samples were tested. The positive rate of Hantavirus in rats was 5.5%. Three strains of Hantavirus were isolated in Vero E6 cell culture. Data from the phylogenetic trees constructed by partial S segment (620-999 nt) or partial G1 segment (180-580 nt) showed that the three isolates carried by rats from Huludao were all genetic subtype SEOV 3. Furthermore, the phylogenetic tree constructed by partial G2 segment (2003-2302 nt) divided SEOV strains into 7 genetic subtypes, and the three isolates were having a closer evolutionary relationship with isolates CP211, ch302 and dc501 from Beijing, and the isolates SD10 and SD227 form Shandong.


CONCLUSIONS
Data indicated that the rate of carrying virus was high and the main genetic subtype of Hantavirus was S3 of Seoul virus in Huludao area.
Qu
Yong-gang
YG
College of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
Yang
Guo-qing
GQ
Zou
Yang
Y
Yan
Gen-qiang
GQ
Chen
Hua-xin
HX
Zhang
Yong-zhen
YZ
D000818:Animals; D002353:Carrier State; D002681:China; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018778:Hantavirus Infections / Q000662:veterinary*; D008168:Lung / Q000821:virology; D010802:Phylogeny; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction