title: [Variable expression of CD28 costimulatory molecule and CTLA4 inhibitory molecule on peripheral blood CD4+ cells in kidney allograft recipients with and without acute graft rejection].
abstract: Alloimune activation is one of the most significant post transplant events, which results in increased expression of costimulatory molecules. These molecules have been suggested to play a role in determining the outcome of immune response including graft rejection.
  
  
  METHODS
  We examined the CD28 and both surface and intracellular CTLA-4 expression on freshly drawn and anti-CD3+rlL-2 stimulated peripheral blood CD4+ T cells in kidney transplant recipients with acute graft rejection and with non-complicated post transplant course. Dual immunofluorescence and flow cytometry methods were used. The proportion of freshly isolated CD4+/ CTLA4 was higher in both groups of graft recipients in comparison to healthy controls reflecting in vivo allostimulation.
  
  
  RESULTS
  We found the increased percentage of CD4+ cells expressing surface CTLA4 after stimulation, unstimulated intracellular CTLA4 and lower percentage of CD4+ cells expressing CD28 after stimulation in kidney recipients without rejection.
  
  
  CONCLUSIONS
  Our results indicate the possible relationship between the expression pattern of CTLA4 inhibitory molecule on CD4+ cells and clinical course after renal transplantation.
authors.affiliation: Akademia Medyczna we Wrocławiu, Katedra i Klinika Nefrologii i Medycyny Transplantacyjnej. klinef@am.centrum.pl
authors.forename: Maria
authors.initials: M
authors.lastname: Magott-Procelewska
authors.forename: Agata
authors.initials: A
authors.lastname: Kosmaczewska
authors.forename: Irena
authors.initials: I
authors.lastname: Frydecka
authors.forename: Lidia
authors.initials: L
authors.lastname: Ciszak
authors.forename: Dariusz
authors.initials: D
authors.lastname: Patrzałek
authors.forename: Piotr
authors.initials: P
authors.lastname: Szyber
authors.forename: Marian
authors.initials: M
authors.lastname: Klinger
mesh_terms: D000208:Acute Disease; D015703:Antigens, CD / Q000096:biosynthesis* / Q000276:immunology; D000943:Antigens, Differentiation / Q000096:biosynthesis* / Q000276:immunology; D018106:CD28 Antigens / Q000096:biosynthesis* / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D060908:CTLA-4 Antigen; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology* / Q000473:pathology; D008297:Male; D012016:Reference Values; D012680:Sensitivity and Specificity; D014184:Transplantation, Homologous / Q000276:immunology* / Q000473:pathology
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D018106:CD28 Antigens; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human