Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells.
The yellow fever virus attenuated 17D vaccine strain is a safe and effective vaccine and a valuable model system for evaluating immune responses against attenuated viral variants. This study compared the in vitro interactions of the commercially available yellow fever vaccine (YF-VAX), Dengue virus and the live-attenuated dengue vaccine PDK50 with dendritic cells (DCs), the main antigen-presenting cells at the initiation of immune responses. Similar to PDK50, infection with YF-VAX generated activated DCs; however, for YF-VAX, activation occurred with limited intracellular virus replication. The majority of internalized virus co-localized with endolysosomal markers within 90 min, suggesting that YF-VAX is processed rapidly in DCs. These results indicate that restricted virus replication and lysosomal compartmentalization may be important contributing factors to the success of the YF-VAX vaccine.
Palmer
Dupeh R
DR
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Fernandez
Stefan
S
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Bisbing
John
J
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Peachman
Kristina K
KK
Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, MD, USA.
Rao
Mangala
M
Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, MD, USA.
Barvir
Dave
D
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Gunther
Vicky
V
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Burgess
Timothy
T
Infectious Disease Directorate, Naval Medical Research Institute, Silver Spring, MD, USA.
Kohno
Yukari
Y
Department of Microbiology and Immunology, Georgetown University School of Medicine, Washington DC, USA.
Padmanabhan
R
R
Department of Microbiology and Immunology, Georgetown University School of Medicine, Washington DC, USA.
Sun
Wellington
W
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
D000818:Animals; D002522:Chlorocebus aethiops; D003713:Dendritic Cells / Q000821:virology*; D006801:Humans; D008247:Lysosomes / Q000378:metabolism* / Q000821:virology; D014709:Vero Cells; D014779:Virus Replication; D022341:Yellow Fever Vaccine / Q000009:adverse effects / Q000276:immunology; D015005:Yellow fever virus / Q000276:immunology / Q000502:physiology*
D022341:Yellow Fever Vaccine