title: Identification and expression of benzylsuccinate synthase genes in a toluene-degrading methanogenic consortium.
abstract: Benzylsuccinate synthase (BSS) initiates anaerobic toluene biodegradation, and BSS genes have been found in several nitrate- and iron-reducing organisms. Here, two new putative bssA genes were identified in a methanogenic toluene-degrading culture. Transcription was upregulated with toluene but not with benzoate, consistent with the proposed function. These are the first bss sequences from a methanogenic culture.
authors.affiliation: Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada M5S 3E5.
authors.forename: Cheryl E
authors.initials: CE
authors.lastname: Washer
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Edwards
mesh_terms: D001483:Base Sequence; D019755:Carbon-Carbon Lyases / Q000302:isolation & purification / Q000378:metabolism*; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D006092:Gram-Negative Facultatively Anaerobic Rods; D008969:Molecular Sequence Data; D014050:Toluene / Q000378:metabolism*
chemical_list: D014050:Toluene; D019755:Carbon-Carbon Lyases; C114354:benzylsuccinate synthase