title: The rolB gene-induced overproduction of resveratrol in Vitis amurensis transformed cells.
abstract: Resveratrol is a stilbene, which prevents carcinogenesis at stages of tumor initiation, promotion and progression. In the present investigation, we developed cell cultures of wild-growing grape (Vitis amurensis Rupr.). The cultures produced low levels of resveratrol, up to 0.026% dry wt., i.e., comparable to levels reported for other plant cell cultures previously established. Different methods commonly used to increase secondary metabolite production (cell selection, elicitor treatments and addition of a biosynthetic precursor) only slightly enhanced cell productivity. Transformation of V. amurensis V2 callus culture by the rolB gene of Agrobacterium rhizogenes resulted in more than a 100-fold increase in resveratrol production in transformed calli. The rolB-transformed calli are capable of producing up to 3.15% dry wt. of resveratrol. We show that the capability to resveratrol biosynthesis is tightly correlated with the abundance of rolB mRNA transcripts. Tyrosine phosphatase inhibitors abolished the rolB-gene-mediated stimulatory effect, thus documenting for the first time the involvement of tyrosine phosphorylation in plant secondary metabolism.
authors.affiliation: Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, 159 Stoletija Str., Vladivostok 690022, Russia. kkv5@mail.ru
authors.forename: K V
authors.initials: KV
authors.lastname: Kiselev
authors.forename: A S
authors.initials: AS
authors.lastname: Dubrovina
authors.forename: M V
authors.initials: MV
authors.lastname: Veselova
authors.forename: V P
authors.initials: VP
authors.lastname: Bulgakov
authors.forename: S A
authors.initials: SA
authors.lastname: Fedoreyev
authors.forename: Y N
authors.initials: YN
authors.lastname: Zhuravlev
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004351:Drug Resistance; D010649:Phenylalanine / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics; D010941:Plant Tumors; D030821:Plants, Genetically Modified; D000077185:Resveratrol; D012231:Rhizobium / Q000235:genetics; D013267:Stilbenes / Q000378:metabolism*; D014162:Transfection; D027843:Vitis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism; D010135:p-Fluorophenylalanine / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D013267:Stilbenes; D010649:Phenylalanine; D010135:p-Fluorophenylalanine; C092241:RolB protein, Agrobacterium rhizogenes; D001617:beta-Glucosidase; D000077185:Resveratrol