The number of G residues in the Bacillus subtilis pyrG initially transcribed region governs reiterative transcription-mediated regulation.
Derepression of pyrG expression in Bacillus subtilis involves CTP-sensitive reiterative transcription, which introduces up to 11 extra G residues at the 5' ends of pyrG transcripts. Insertion of three or more additional Gs at the 5' end of the pyrG initially transcribed region abolished reiterative transcription and caused constitutive expression.
Elsholz
Alexander K W
AK
Department of Biochemistry, University of Illinois, Urbana, IL 61801, USA.
Jørgensen
Casper Møller
CM
Switzer
Robert L
RL
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D019731:Carbon-Nitrogen Ligases / Q000235:genetics* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D014158:Transcription, Genetic
D019731:Carbon-Nitrogen Ligases; C019951:CTP synthetase