Nondestructive micro-patterning of proteinous occlusion bodies in water by femtosecond laser-induced mechanical force.
Some insect virus produces proteinous occlusion bodies named polyhedra, on which it is possible to immobilize functional proteins, such as green fluorescent proteins, antibodies, and growth factors, with keeping their biological activity. In this work, several kinds of polyhedra were micro-patterned by applying a mechanical force induced by femtosecond laser irradiation of a water buffer. In the printing process, since the laser is not directly irradiated on polyhedra, damages of polyhedra due to dryness and photothermal and photochemical reactions will be suppressed. The ability of the pattering was demonstrated by micro-sized checkerboard pattern, on which polyhedra with and without occluding enhanced green fluorescent protein were sorted. Furthermore, it was indicated that polyhedra are available as a scaffold of animal cell. This patterning method will be a promising technique to realize a microdevice in which functions of cell are artistically activated.
Hosokawa
Yoichiroh
Y
Department of Applied Physics and Venture Business Lab., Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. hosokawa@ap.eng.osaka-u.ac.jp
Kaji
Takahiro
T
Shukunami
Chisa
C
Hiraki
Yuji
Y
Kotani
Eiji
E
Mori
Hajime
H
Masuhara
Hiroshi
H
D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D002479:Inclusion Bodies / Q000737:chemistry*; D007834:Lasers; D008422:Materials Testing; D019563:Mechanics; D051379:Mice; D041681:NIH 3T3 Cells; D011506:Proteins / Q000737:chemistry*; D013314:Stress, Mechanical; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D011506:Proteins; D014867:Water