Expression of regulatory T-lymphocyte phenotype in human fetal hemopoietic and lymphoid cell culture.
Cells with regulatory T-cell phenotype (Treg, CD4+CD25(hi)) were not detected in human fetal thymus, liver, bone marrow, spleen, and among blood mononuclears of 14-28-week gestation. The cells of the majority of these fetal thymuses express Treg specific marker (FOXP3 transcription marker) gene. Culturing of fetal liver and bone marrow cells on a monolayer of thymic epithelial cells induced expression of FOXP3 gene, but induction of CD4+CD25+ membrane phenotype was detected in only 1 of 8 studied cultures (in liver and bone marrow cells). Induction of Treg differentiation is to a greater extent determined by the characteristics of hemopoietic organ cells than of thymic epithelial cells.
Sharova
N I
NI
Institute of Immunology, FMBA.
Donetskova
A D
AD
Dubrovina
I V
IV
Sukhikh
G T
GT
Yarilin
A A
AA
D015704:CD4 Antigens / Q000096:biosynthesis; D017931:DNA Primers / Q000737:chemistry; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006413:Hematopoietic System; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis; D008214:Lymphocytes / Q000166:cytology*; D010641:Phenotype; D050378:T-Lymphocytes, Regulatory / Q000473:pathology*; D013997:Time Factors; D014018:Tissue Distribution
D015704:CD4 Antigens; D017931:DNA Primers; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases