Functional aftereffects of intraparenchymatous injection of human fetal stem and progenitor cells to rats with chronic and acute renal failure.
Chronic renal insufficiency was modeled in rats by unilateral nephrectomy and electrocoagulation of both poles of the remaining kidney; acute renal failure was induced by 90-min clamping of the vascular pedicle of the only kidney. Injection of unfractionated culture of human fetal kidney cells or bone marrow mesenchymal stem cells into damaged kidney restored its function in rats with chronic renal insufficiency (observation period up to 2 months). After 2.5 months a relapse of chronic renal insufficiency was observed in 1 of 3 rats receiving human fetal kidney cells and in 1 of 2 animals receiving bone marrow mesenchymal stem cell culture. Injection of bone marrow mesenchymal stem cell culture to rats with acute renal failure improved recovery of renal function and prevented the death from uremia, while injection of total culture of human fetal kidney cells had virtually no effect on the course of acute renal failure.
Kirpatovskii
V I
VI
Institute of Urology, Russian Ministry of Health, Moscow. vladkir@post.ru
Kazachenko
A V
AV
Plotnikov
E Yu
EY
Marei
M V
MV
Musina
R A
RA
Kon'kova
T A
TA
Drozhzheva
V V
VV
Nadtochii
O N
ON
Sukhikh
G T
GT
D000208:Acute Disease; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D006801:Humans; D007511:Ischemia / Q000473:pathology; D007668:Kidney / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000628:therapy*; D008648:Mesoderm / Q000378:metabolism; D051381:Rats; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013997:Time Factors