Highly sensitive assay for the measurement of serotonin in microdialysates using capillary high-performance liquid chromatography with electrochemical detection.
A highly sensitive isocratic capillary high-performance liquid chromatographic (HPLC) method with electrochemical detection (ED) for the simultaneous measurement of serotonin (5-hydroxytryptamine, 5-HT) and its metabolite 5-hydroxyindole-3-acetic acid (5-HIAA) in microdialysates has been developed using a 0.5 mm i.d. capillary column and a 11-nL detection cell. This method, validated on both pharmacological and analytical bases, can be performed using injection volumes as low as 1 microL. The limits of detection were 5.6 x 10(-11)mol/L and 3.0 x 10(-9)mol/L for 5-HT and 5-HIAA. Several applications of the present method are given on microdialysates from rodent brain and human spinal cord.
Parrot
Sandrine
S
NEUROCHEM, Institut Fédératif des Neurosciences de Lyon, Plate-forme de Physiologie, Université Lyon 1, France. sparrot@sante.univ-lyon1.fr
Lambás-Señas
Laura
L
Sentenac
Sabine
S
Denoroy
Luc
L
Renaud
Bernard
B
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D017551:Microdialysis; D012680:Sensitivity and Specificity; D012701:Serotonin / Q000378:metabolism*
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid