Mapping the proteome of poplar and application to the discovery of drought-stress responsive proteins.
Poplar is the first forest tree genome to be decoded. As an initial step to the comprehensive analysis of poplar proteome, we described reference 2-D-maps for eight tissues/organs of the plant, and the functional characterization of some proteins. A total of 398 proteins were excised from the gels. About 91.2% were identified by nanospray LC-MS/MS, based on comparison with 260,000 Populus sp. ESTs. In comparison, reliable PMFs were obtained for only 51% of the spots by MALDI-TOF-MS, from which 43% (83 spots) positively matched gene models of the Populus trichocarpa genome sequence. Among these 83 spots, 58% matched with the same proteins as identified by LC-MS/MS, 21.7% with unknown function proteins and 19.3% with completely different functions. In the second phase, we studied the effect of drought stress on poplar root and leaf proteomes. The function of up- and down-regulated proteins is discussed with respect to the physiological response of the plants and compared with transcriptomic data. Some important clues regarding the way poplar copes with water deficit were revealed.
Plomion
Christophe
C
UMR Biodiversité Gènes Communautés, INRA, Equipe de génétique, Cestas, France. plomion@pierroton.inra.fr
Lalanne
Céline
C
Claverol
Stéphane
S
Meddour
Hakim
H
Kohler
Annegret
A
Bogeat-Triboulot
Marie-Béatrice
MB
Barre
Aurélien
A
Le Provost
Grégoire
G
Dumazet
Hélène
H
Jacob
Daniel
D
Bastien
Catherine
C
Dreyer
Erwin
E
de Daruvar
Antoine
A
Guehl
Jean-Marc
JM
Schmitter
Jean-Marie
JM
Martin
Francis
F
Bonneu
Marc
M
D030562:Databases, Protein; D004190:Disasters; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D020411:Oligonucleotide Array Sequence Analysis; D010449:Peptide Mapping / Q000379:methods; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D032107:Populus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods* / Q000706:statistics & numerical data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014158:Transcription, Genetic; D015854:Up-Regulation
D010940:Plant Proteins