Chemical synthesis of oxanine-contained oligodeoxynucleotides.
Oxanine, one of major damaged product from deamination of guanine base mediated by NO or HNO2-induced nitrosative reaction, has been considered as a genotoxic lesion. To analyze the biophysical properties of oxanine in context of oligodeoxynucleotide, the chemical incorporation of 2'-deoxyoxanosine (dOxo, nucleoside form of oxanine) into oligodeoxynucleotide is required. Here, we developed the chemical synthesis procedures of dimethoxytritylated dOxo-phosphoramidite (DMT-dOxo-amidite) and oxanine-contained oligonucleotide.
Pack
Seung Pil
SP
International Innovation Center, Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto 606-8501, Japan.
Yamamoto
Daihei
D
Kodaki
Tsutomu
T
Makino
Keisuke
K
D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D011684:Purine Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry
C504691:5'-O-(4,4'-dimethoxytrityl)-dOxo-amidite; D009838:Oligodeoxyribonucleotides; D011684:Purine Nucleosides; C468766:oxanine