Formation of dendrimeric RNA assemblies though RNA loop-loop interactions.
We describe the construction of nano-scale dendritic assemblies using RNA building blocks based on dimerization initiation site (DIS) RNA of the human immunodeficiency virus type 1 (HIV-1) that forms stable base pairing through loop-loop ("kissing") interactions. RNA building blocks containing three DIS-like hairpins connected by a two nucleotide linker self-assembled in a magnesium-dependent manner to form dendrimeric structures as observed by non-denaturing polyacrylamide gel electrophoresis (PAGE).
Fujiya
Isamu
I
Department of Life Science, Tokyo Gakugei University, Koganei, Tokyo 184-8501, Japan.
Harada
Kazuo
K
D001483:Base Sequence; D050091:Dendrimers / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D015497:HIV-1 / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D050091:Dendrimers; D012367:RNA, Viral