title: Deamination of 2',3'-O-isopropylideneadenosine-5'- carboxylic acid catalyzed by adenosine deaminase (ADA) and adenylate deaminase (AMPDA): influence of substrate ionization on the activity of the enzymes.
abstract: Adenosine deaminase (ADA) and adenylate deaminase (AMPDA) catalyze the deamination of 2 ',3 '-O-isopropylideneadenosine-5'-carboxylic acid to the corresponding inosine derivative and dependence of the rate of enzymatic reaction on the ionization degree of the substrate has been studied at different pH values.
authors.affiliation: Dipartimento di Scienze Precliniche LITA Vialba, Università degli Studi di Milano, Milano, Italy.
authors.forename: Pierangela
authors.initials: P
authors.lastname: Ciuffreda
authors.forename: Laura
authors.initials: L
authors.lastname: Alessandrini
authors.forename: Radmila
authors.initials: R
authors.lastname: Pavlovic
authors.forename: Enzo
authors.initials: E
authors.lastname: Santaniello
mesh_terms: D000659:AMP Deaminase / Q000737:chemistry*; D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry; D000243:Adenosine Deaminase / Q000737:chemistry*; D002384:Catalysis; D003641:Deamination; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: C518810:2',3'-O-isopropylideneadenosine-5'-carboxylic acid; D007477:Ions; D000243:Adenosine Deaminase; D000659:AMP Deaminase; D000241:Adenosine