Lipopeptide-based liposomes for DNA delivery into cells expressing neuropilin-1.
In this paper, liposomes containing a lipopeptide bearing a ligand specifically recognized by neuropilin-1 (NRP-1) have been used to target a human breast cancer cell line overexpressing this receptor. The synthesis of this lipopeptide, C16-A7R, formed by the sequence of 7 amino acids ATWLPPR, linked to a palmitoyl fatty chain by an amide bond was described. After the characterisation of cationic liposomes formulated with the lipopeptide, the results obtained using various techniques showed that the lipopeptide-based liposomes were well accumulated in cells of the human breast cancer line MDA-MB-231 overexpressing NRP-1. Delivery of reporter genes expressing either beta-galactosidase (beta-gal) or green fluorescent protein (GFP) was selectively enhanced in these cells when compared with NRP-1-negative cells. In MDA-MB-231 cells, an increase by 250% in beta-gal activity was observed when delivered by lipopeptide-based liposomes compared to cationic liposomes alone.
Slimani
Hocine
H
Laboratoire de Biophysique Moléculaire Cellulaire et Tissulaire, CNRS UMR 7033, UFR de Médecine, Université Paris 13, 74 rue Marcel Cachin, F93017, Bobigny Cedex, France.
Guenin
Erwann
E
Briane
Dominique
D
Coudert
Robert
R
Charnaux
Nathalie
N
Starzec
Anna
A
Vassy
Roger
R
Lecouvey
Marc
M
Perret
Yves Gerard
YG
Cao
An
A
D015153:Blotting, Western; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D003587:Cytomegalovirus / Q000235:genetics; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics*; D005434:Flow Cytometry; D017930:Genes, Reporter / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000737:chemistry; D007202:Indicators and Reagents; D008081:Liposomes / Q000737:chemistry*; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D039942:Neuropilin-1 / Q000096:biosynthesis* / Q000235:genetics*; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D010957:Plasmids / Q000235:genetics; D013778:Tetrazolium Salts; D013844:Thiazoles; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics
D007202:Indicators and Reagents; D008081:Liposomes; D010455:Peptides; D013778:Tetrazolium Salts; D013844:Thiazoles; D039942:Neuropilin-1; D049452:Green Fluorescent Proteins; D004247:DNA; D001616:beta-Galactosidase; C022616:thiazolyl blue