title: [Synthesis of xylanolytic enzymes and other carbohydrases by the fungus Penicillium canescens: transformants with an altered copy number of the gene xlnR and an encoding transcriptional activator].
abstract: A fragment of Penicillium canescens genomic DNA carrying the xlnR gene coding for a translational activator of xylanolytic genes was isolated. It was demonstrated that a loss of this function in genetically modified transformants resulted in a drastic decrease in the production of P. canescens major xylanases and had a negative effect on the syntheses of several other extracellular xylanases. An increase in the dose of the xlnR gene elevated the xylanolytic activity as well as the activities of a number of other auxiliary enzymes involved in xylan degradation. The activities of two P. canescens major secreted enzymes--beta-galactosidase and alpha-L-arabinofuranosidase-appeared weakly dependent on the translational activator xlnR.
authors.forename: V A
authors.initials: VA
authors.lastname: Serebrianyĭ
authors.forename: O A
authors.initials: OA
authors.lastname: Sinitsyna
authors.forename: E A
authors.initials: EA
authors.lastname: Fedorova
authors.forename: O N
authors.initials: ON
authors.lastname: Okunev
authors.forename: A O
authors.initials: AO
authors.lastname: Bekkarevich
authors.forename: L M
authors.initials: LM
authors.lastname: Sokolova
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Vinetskiĭ
authors.forename: A P
authors.initials: AP
authors.lastname: Sinitsyn
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017353:Gene Deletion; D018628:Gene Dosage; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D016681:Genome, Fungal; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology*; D014170:Transformation, Genetic; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D015534:Trans-Activators; D014990:Xylans; D006026:Glycoside Hydrolases; D014995:Xylosidases; C019822:carbohydrase