title: Suppression of bcr/abl chimeric gene by conjugate DNA enzymes in human cells.
abstract: Conjugate DNAzymes with polyamines and peptides were successfully prepared by solid phase fragment condensation (SPFC) and showed up to 4.2 times higher catalytic efficiency (kcat/Km). Intracellular localization of DNAzymes could be controlled by conjugated with naturally occurring signal peptides which are responsible for nuclear cytoplasmic transport of proteins. Suppression of bcr/abl chimeric gene on Philadelphia chromosome by conjugate DNA enzymes were largely enhanced in human leukemia cells.
authors.affiliation: Department of Biological and Environmental Chemistry, Kyushu School of Engineering, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Takamori
authors.forename: Takanori
authors.initials: T
authors.lastname: Kubo
authors.forename: Zhivko
authors.initials: Z
authors.lastname: Zhelev
authors.forename: Bakalova
authors.initials: B
authors.lastname: Rumiana
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohba
authors.forename: Keiko
authors.initials: K
authors.lastname: Doi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fujii
mesh_terms: D002384:Catalysis; D021881:DNA, Catalytic / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D016044:Fusion Proteins, bcr-abl / Q000037:antagonists & inhibitors / Q000235:genetics*; D016313:Genes, abl; D006801:Humans; D020014:K562 Cells; D007700:Kinetics; D011073:Polyamines / Q000737:chemistry; D021382:Protein Sorting Signals; D012313:RNA / Q000378:metabolism
chemical_list: D021881:DNA, Catalytic; D011073:Polyamines; D021382:Protein Sorting Signals; C406060:abl-bcr fusion protein, human; D012313:RNA; D016044:Fusion Proteins, bcr-abl