Isolation of bisphenol A-tolerant/degrading Pseudomonas monteilii strain N-502.
Bisphenol A (BPA) is a highly biotoxic compound that kills many microorganisms at a low concentration (1,000 ppm). We isolated BPA-tolerant/degrading Pseudomonas monteilii strain N-502 from about 1,000 samples collected from a field, sewage, and pond water. The isolated strain had strong BPA tolerance and high BPA-degrading activity. This strain was able to grow in a minimum medium containing BPA as the sole carbon source. Strain N-502 is an aerobic, motile, gram-negative, nonspore-forming, rod-shaped bacterium and was identified as P. monteilii, based on 16 S rRNA gene analysis. Strain N-502 completely degraded BPA 500 ppm in a 10-day, in culture system and was able to degrade BPA 100 ppm in a 2-h resting cell system. This strain also showed potent ability to degrade BPA 500 and 1,000 ppm in the resting cell system. Moreover, the initial BPA degradation rate was accelerated with the addition of Ca(2+), Mg(2+), and folic acid.
Masuda
Midori
M
Graduate School of Life Science, Toyo University, Itakura-machi, Oura-gun, Gunma, 374-0193, Japan. dx0300067@toyonet.toyo.ac.jp
Yamasaki
Yoshiki
Y
Ueno
Shun
S
Inoue
Akira
A
D001559:Benzhydryl Compounds; D001711:Biotransformation / Q000502:physiology; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D010636:Phenols / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010636:Phenols; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds