Reduced lipopolysaccharide (LPS)-induced nitric oxide production in peritoneal macrophages and inhibited LPS-induced lethal shock in mice by a sugar cane (Saccharum officinarum L.) extract.
A sugar cane extract (SCE) has been found to have an immunostimulating effect in several animals. Lipopolysaccharide (LPS) is known to induce endotoxin shock via the production of inflammatory modulators such as tumor necrosis factor (TNF)-alpha and nitric oxide (NO). We examined in the present study the effects of SCE on the TNF-alpha and NO production in LPS-stimulated mice peritoneal cells and the endotoxin shock in mice. The supplementation of SCE to peritoneal macrophages cultured with LPS resulted in a significant decrease in NO production. All the mice injected intraperitoneally with LPS and D-galactosamine (LPS+GalN) died within 24 h. However, a peritoneal injection, but no intravenous or oral administration, of SCE (500-1,000 mg/kg) at 3 to 48 h before the LPS+GalN-challenge resulted in a significantly improved survival rate. These results suggest that SCE had a protective effect on LPS-induced endotoxin shock via one of possible mechanisms involving the suppression of NO production in the mouse peritoneal cavity.
Hikosaka
Kenji
K
National Institute of Animal Health, Tsukuba, Ibaraki.
Koyama
Yukari
Y
Motobu
Maki
M
Yamada
Manabu
M
Nakamura
Kikuyasu
K
Koge
Kenji
K
Shimura
Kameo
K
Isobe
Takashi
T
Tsuji
Naotoshi
N
Kang
Chung-Boo
CB
Hayashidani
Hideki
H
Wang
Pi-Chao
PC
Matsumura
Masatoshi
M
Hirota
Yoshikazu
Y
D000818:Animals; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis*; D010936:Plant Extracts / Q000494:pharmacology*; D031786:Saccharum / Q000737:chemistry*; D015996:Survival Rate
D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide