Chemical behavior of bis(3'-5')diguanylic acid in aqueous solutions.
This paper describes unique behavior of bis(3'-5')diguanylic acid (c-di-GMP) under some conditions. Thus, c-di-GMP exists as the monomer in aprotic organic solvents such as DMSO. By contrast, c-di-GMP smoothly aggregates in water and in low-concentration aqueous solutions of some salts, such as sodium chloride and ammonium acetate, to give a mixture of many aggregates. The resulting multiple aggregates converge to the single compound (provably the monomer) in a >154 mM (0.9%) sodium chloride aqueous solution, in a >100 mM ammonium acetate buffer, and in a >100 mM phosphate buffer.
Hyodo
Mamoru
M
Graduate School of Information Science/Human Informatics, and CREST/JST, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Sato
Yumi
Y
Hayakawa
Yoshihiro
Y
Karaolis
David K R
DK
D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D012996:Solutions; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
D002021:Buffers; D012996:Solutions; D012997:Solvents; D014867:Water; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP