Purification and characterization of a corrinoid-compound in an edible cyanobacterium Aphanizomenon flos-aquae as a nutritional supplementary food.
The vitamin B12 concentration of the dried cells of Aphanizomenon flos-aquae was determined by both microbiological method with Lactobacillus delbrueckeii ATCC7830 and chemiluminescence method with intrinsic factor. The Aphanizomenon cells contained 616.3 +/- 30.3 micro g (n = 4) of vitamin B12 per 100 g of the dried cells by the microbiological method. The values determined with the chemiluminescence method, however, were only about 5.3% of the values determined by the microbiological method. A corrinoid-compound was purified from the dried cells and characterized. The purified corrinoid-compound was identified as pseudovitamin B12 (an inactive corrinoid-compound for humans) by silica gel 60 TLC, C18 reversed-phase HPLC, ultraviolet-visible spectroscopy, and 1H NMR spectroscopy. The results suggest that the Aphanizomenon cells are not suitable for use as a vitamin B12 source, especially in vegans.
Miyamoto
Emi
E
Department of Health and Nutrition, Nagasaki International University, Sasebo, Japan. miyamo@niu.ac.jp
Tanioka
Yuri
Y
Nakao
Tomoyuki
T
Barla
Florin
F
Inui
Hiroshi
H
Fujita
Tomoyuki
T
Watanabe
Fumio
F
Nakano
Yoshihisa
Y
D046890:Aphanizomenon / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D045728:Corrinoids / Q000302:isolation & purification*; D019587:Dietary Supplements; D009682:Magnetic Resonance Spectroscopy; D014805:Vitamin B 12 / Q000032:analysis
D045728:Corrinoids; D014805:Vitamin B 12