title: Electrochemical detection of DNase I activity.
abstract: DNase I in one microl of the water could quantitate electrochemically with the detection limit of 0.01 units (ca. 20 pg) by using the ferrocenyl oligonucleotide-immobilized electrode prepared by thiolated oligonucleotide and ferrocenyl carbodiimide as a simple labeling reagent of redox unit.
authors.affiliation: Department of Materials Science, Faculty of Engineering, Kyushu Institute of Technology, 1-1 Sensui-cyo, Tobata-ku, Kitakyushu-shi, Fukuoka 804-8550, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Fujita
authors.forename: Masanori
authors.initials: M
authors.lastname: Kanazawa
authors.forename: Kosuke
authors.initials: K
authors.lastname: Mukumoto
authors.forename: Takahiko
authors.initials: T
authors.lastname: Nojima
authors.forename: Shinobu
authors.initials: S
authors.lastname: Sato
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kondo
authors.forename: Michinori
authors.initials: M
authors.lastname: Waki
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D002234:Carbodiimides / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D010084:Oxidation-Reduction; D013873:Thionucleotides / Q000737:chemistry
chemical_list: D002234:Carbodiimides; D005296:Ferrous Compounds; D009841:Oligonucleotides; D013873:Thionucleotides; C517870:ferrocenyl carbodiimide; D003850:Deoxyribonuclease I