[Establishment of autologous endometrial coculture and sequential system for human early embryo culture].
OBJECTIVE
To establish a coculture and a sequential system for human early embryo culture.


METHODS
The endometrial tissue was digested enzymatically and cultured to achieve generated and cryo-thawed endometrial monolayer cells. The generated and cryo-thawed monolayer cells were cocultured with human 2PN embryos and transferred to sequential medium every 48 hours.


RESULTS
Human endometrial cells had viability in vitro culture. The autologously generated and cryo-thawed monolayer cells were successfully obtained, and 74.04% of the cryo-thawed cells were successfully used in coculturing human early embryos. The embryos developed well, with the clinical pregnancy rate of 68.83% and the implantation rate of 44.23%.


CONCLUSIONS
The autologous endometrial cell coculture and sequential culture system for human early embryo development provides a feasible method for studying human embryo development and implantation so as to improve embryo quality.
Zhang
Ning-yuan
NY
Reproductive Medicine Center, Drum Tower Hospital Affiliated to Nanjing University Medical College, Nanjing, Jiangsu 210008, China.
Hu
Ya-li
YL
Sun
Hai-xiang
HX
Wang
Bin
B
Xu
Zhi-peng
ZP
Chen
Hua
H
D002478:Cells, Cultured; D018920:Coculture Techniques; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology*; D004717:Endometrium / Q000166:cytology*; D005260:Female; D006801:Humans
D003470:Culture Media