Variovorax soli sp. nov., isolated from greenhouse soil.
A Gram-negative, rod-shaped, non-spore-forming bacterium, strain GH9-3(T), isolated from greenhouse soil, was investigated in a polyphasic study. The novel organism grew at 10-35 degrees C, 0-3 % NaCl and pH 5-9. It had ubiquinone 8 (Q-8) as the predominant isoprenoid quinone and possessed C(16 : 0), summed feature 3, C(17 : 0) cyclo and C(18 : 1) omega7c as the major fatty acids (together representing 87.4 % of the total). The DNA G+C content was 67.1 mol%. 16S rRNA gene sequence analysis of strain GH9-3(T) showed that it grouped within the Variovorax cluster, with highest sequence similarities to Variovorax paradoxus IAM 12373(T) (98.3 %) and Variovorax dokdonensis DS-43(T) (98.0 %). DNA-DNA hybridization values between strain GH9-3(T) and V. paradoxus DSM 30034(T) and V. dokdonensis DS-43(T) were 38 and 29 %, respectively. Based on phenotypic, chemotaxonomic and phylogenetic features, it is proposed that strain GH9-3(T) represents a novel species of the genus Variovorax with the name Variovorax soli sp. nov. The type strain is GH9-3(T) (=KACC 11579(T)=DSM 18216(T)).
Kim
Byung-Yong
BY
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Weon
Hang-Yeon
HY
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Yoo
Seung-Hee
SH
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Lee
Seon-Young
SY
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Kwon
Soon-Wo
SW
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Go
Seung-Joo
SJ
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Stackebrandt
Erko
E
Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
D015373:Bacterial Typing Techniques; D042621:Comamonadaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D007723:Korea; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S