[Studies on extraction, isolation and composition of Lycium barbarum polysaccharides].
OBJECTIVE
To study the extraction, isolation and composition of Lycium barbarum polysaccharides (LBP).


METHODS
LBP was extracted from L. barbarum with water, isolationed and purified by DEAE ion-exchange cellulose and gel chromatography, and their structural composition was studied by means of SDS-PAGE gel electrophoresis, GC, amino acid automatic analysis, etc.


RESULTS
Pure LBP has four water solubie polysaccharides, M W was 1.524 x 10(5). LBP was composed of 6 kinds of monosaccharides (Ara, Rha, Xyl, Man, Gal and Glc), galacturonic acid and 18 kinds of amino acids.


CONCLUSIONS
LBP is a kind of complex polysaccharides consisting of acidic heteropolysaccharides and polypeptide or protein, and LBP has Glycan-O-Ser glycopeptide structures.
Tian
Meili
M
School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.
Wang
Min
M
D000596:Amino Acids / Q000032:analysis*; D006603:Hexuronic Acids / Q000032:analysis*; D032305:Lycium / Q000737:chemistry*; D009005:Monosaccharides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry
D000596:Amino Acids; D006603:Hexuronic Acids; D009005:Monosaccharides; D011134:Polysaccharides; C007819:galacturonic acid