title: [Study of mutation and single nucleotide polymorphism of PDGFRbeta and SHIP gene in acute myeloid leukemia].
abstract: OBJECTIVE
  To investigate the significance of mutation and single nucleotide polymorphism (SNP) of class III receptor tyrosine kinases such as PDGFRbeta and SHIP in acute myeloid leukemia (AML) patients.
  
  
  METHODS
  Screening of the mutation and SNP of PDGFRbeta and SHIP by genomic PCR, RT-PCR, directly sequencing and Mass-ARRAY system was carried out in 273 AML patients.
  
  
  RESULTS
  The mutations of PDGFRbeta R685C and SHIP Q1153L were detected for the first time in AML patients. The positivity ratio was 0.73% and 0.36% respectively.
  
  
  CONCLUSIONS
  The mutations of PDGFRbeta R685C and SHIP Q1153L may contribute to leukemogenesis of AML.
authors.affiliation: Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Su-jiang
authors.initials: SJ
authors.lastname: Zhang
authors.forename: Jian-yong
authors.initials: JY
authors.lastname: Li
authors.forename: Jing-yi
authors.initials: JY
authors.lastname: Shi
authors.forename: Zhan-zhong
authors.initials: ZZ
authors.lastname: Shi
authors.forename: Bai-wei
authors.initials: BW
authors.lastname: Gu
authors.forename: Xue-tao
authors.initials: XT
authors.lastname: Bai
authors.forename: Yong-mei
authors.initials: YM
authors.lastname: Zhu
authors.forename: Zhi-xiang
authors.initials: ZX
authors.lastname: Shen
mesh_terms: D006801:Humans; D007295:Inositol Phosphates / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D013058:Mass Spectrometry; D009154:Mutation; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007295:Inositol Phosphates; D020797:Receptor, Platelet-Derived Growth Factor beta