title: The isolation of 1,2,3,4,6-penta-O-galloyl-beta-D-glucose from Acer truncatum Bunge by high-speed counter-current chromatography.
abstract: High-speed counter-current chromatography (HSCCC) was successfully used for the isolation and purification of 1,2,3,4,6-penta-O-galloyl-beta-D-glucose from the ethyl acetate extract of the leaves of Acer truncatum Bunge using a two-phase system composed of n-hexane-ethyl acetate-methanol-water at a volume ratio of (0.25:5:1:5, v/v/v/v) for the first time. Each injection of 80 mg crude extract yielded 7.25 mg of pure 1,2,3,4,6-penta-O-galloyl-beta-D-glucose. High-performance liquid chromatography (HPLC) analyses of the CCC fraction revealed that the purity of 1,2,3,4,6-penta-O-galloyl-beta-D- glucose was over 95%.
authors.affiliation: Department of Chemical Biology, Capital Medical University, Beijing 100069, China.
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Zhao
authors.forename: Chun-Chun
authors.initials: CC
authors.lastname: Gao
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Ma
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Bai
authors.forename: Ying-Xia
authors.initials: YX
authors.lastname: Zhang
mesh_terms: D031002:Acer / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods*; D047348:Hydrolyzable Tannins / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D021241:Spectrometry, Mass, Electrospray Ionization; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D047348:Hydrolyzable Tannins; C435084:pentagalloylglucose