Chemistry-based RNA technologies: demonstration of usefulness of libraries of ribozymes and short hairpin RNAs (shRNAs).
Mechanism of action of hammerhead ribozymes has been investigated and their intracellular activities have been improved. Based on the improved ribozymes and more recently discovered natural RNAi, we have created libraries of both ribozymes and short hairpin RNAs (shRNAs). The introduction of a library of active ribozymes or shRNAs into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function.
Miyagishi
Makoto
M
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-8656, Japan.
Matsumoto
Sahohime
S
Akashi
Hideo
H
Kawasaki
Hiroaki
H
Fukao
Taro
T
Fukuda
Yoko
Y
Sano
Masayuki
M
Kato
Yoshio
Y
Takagi
Yasuomi
Y
Tanaka
Yoshiyuki
Y
Warashina
Masaki
M
Kuwabara
Tomoko
T
Sawata
Shinya Y
SY
Ikeda
Yutaka
Y
Kawahara
Shun-ichi
S
Sunil
Kaul C
KC
Wadhwa
Renu
R
Taira
Kazunari
K
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D020650:Combinatorial Chemistry Techniques; D006801:Humans; D051379:Mice; D010641:Phenotype; D034622:RNA Interference; D016337:RNA, Catalytic / Q000737:chemistry*; D022661:RNA, Untranslated / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D016337:RNA, Catalytic; D022661:RNA, Untranslated; C427204:hammerhead ribozyme