Hyaluronic acid hydrogel immobilized with RGD peptides for brain tissue engineering.
In this paper, hyaluronic acid hydrogels with open porous structure have been developed for scaffold of brain tissue engineering. A short peptide sequence of arginine-glycine-aspartic acid (RGD) was immobilized on the backbone of the hydrogels. Both unmodified hydrogels and those modified with RGD were implanted into the defects of cortex in rats and evaluated for their ability to improve tissue reconstruction. After 6 and 12 weeks, sections of brains were processed for DAB and Glees staining. They were also labeled with GFAP and ED1 antibodies, and observed under the SEM for ultrastructral examination. After implanting into the lesion of cortex, the porous hydrogels functioned as a scaffold to support cells infiltration and angiogenesis, simultaneously inhibiting the formation of glial scar. In addition, HA hydrogels modified with RGD were able to promote neurites extension. Our experiments showed that the hyaluronic acid-RGD hydrogel provided a structural, three-dimensional continuity across the defect and favoured reorganization of local wound-repair cells, angiogenesis and axonal growth into the hydrogel scaffold, while there was little evidence of axons regeneration in unmodified hydrogel.
Cui
F Z
FZ
Biomaterials Laboratory, Department of Materials Science & Engineering, Tsinghua University, Beijing 100084, China. cuifz@mail.tsinghua.edu.cn
Tian
W M
WM
Hou
S P
SP
Xu
Q Y
QY
Lee
I-S
IS
D020341:Absorbable Implants; D000818:Animals; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology / Q000601:surgery*; D002465:Cell Movement / Q000502:physiology; D005260:Female; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000627:therapeutic use / Q000648:ultrastructure; D020100:Hydrogels / Q000008:administration & dosage* / Q000627:therapeutic use; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides / Q000008:administration & dosage* / Q000627:therapeutic use; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering
D020100:Hydrogels; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D006820:Hyaluronic Acid