title: Novel fluorescent analogues for transmembrane movement study of polyprenyl phosphates.
abstract: In order to investigate the transmembrane movement of polyprenyl phosphate across biological membranes, NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled polyprenyl phosphate analogues were prepared. These analogues proved to be possible tools for a direct observation of the transmembrane flip-flop movement of polyprenyl phosphates by use of a sodium dithionite-quenching procedure.
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai 980-8577, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Koseki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamashita
authors.forename: Seiji
authors.initials: S
authors.lastname: Takahashi
authors.forename: Tanetoshi
authors.initials: T
authors.lastname: Koyama
mesh_terms: D055592:Biophysical Phenomena; D001703:Biophysics; D004227:Dithionite / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D005615:Freezing; D007202:Indicators and Reagents; D008081:Liposomes; D008566:Membranes / Q000378:metabolism / Q000648:ultrastructure; D008567:Membranes, Artificial; D010710:Phosphates / Q000138:chemical synthesis*; D010713:Phosphatidylcholines; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008081:Liposomes; D008567:Membranes, Artificial; D010710:Phosphates; D010713:Phosphatidylcholines; D004227:Dithionite