Ethnic differences in the distribution of CYP3A5 gene polymorphisms.
The genetic polymorphism affecting the CYP3A5 enzyme is responsible for interindividual and interethnic variability in the metabolism of CYP3A5 substrates. The full extent of the CYP3A5 genetic polymorphism was analysed in French Caucasian, Gabonese and Tunisian populations using a polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) strategy. In the three populations, eight, 17 and ten single nucleotide polymorphisms (SNPs), respectively, were identified, among which nine correspond to rare new mutations. Also identified were 16 alleles including eight new allelic variants. Significant differences were observed in the distribution of these alleles. Particularly, the frequency of the CYP3A5*3C null allele in French Caucasians (81.3%) and in Tunisians (80.0%) is higher than in the Gabonese population (12.5%) (p < 0.001). Considering the CYP3A5 genotypes of the tested individuals, only 10.4% of French Caucasians and 30.0% of Tunisians were identified as CYP3A5 expressors. In contrast, 90.0% of Gabonese subjects appear to express the CYP3A5 protein.
Quaranta
S
S
Equipe d'Accueil 2679, Facultés de Médecine et de Pharmacie de Lille, Pôle Recherche, Lille, France. sylvie_quaranta@hotmail.com
Chevalier
D
D
Allorge
D
D
Lo-Guidice
J M
JM
Migot-Nabias
F
F
Kenani
A
A
Imbenotte
M
M
Broly
F
F
Lacarelle
B
B
Lhermitte
M
M
D000483:Alleles; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics*; D005602:France; D005681:Gabon; D005787:Gene Frequency; D006801:Humans; D010641:Phenotype; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D014416:Tunisia; D044465:White People / Q000235:genetics
D003577:Cytochrome P-450 Enzyme System; C510164:CYP3A5 protein, human; D051544:Cytochrome P-450 CYP3A