NMR spectroscopic analyses of functional nucleic acids-metal interaction and their solution structure analyses.
We have studied complexations of functional nucleic acids and metal ions, by means of NMR spectroscopy. In the case of hammerhead ribozymes, they have a metal ion-binding motif in their core sequences. Upon the metallation of N7 of a guanosine in an RNA duplex modelled after hammerhead ribozymes, 20 ppm higher field shift of N7 was observed in 1-dimensional (1D) 15N NMR spectra. It was found that metal ion binding to nucleobases were detectable with 15N NMR spectroscopy.
Tanaka
Yoshiyuki
Y
Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-8578, Japan.
Yamaguchi
Hiroshi
H
Oda
Shuji
S
Kojima
Chojiro
C
Ono
Akira
A
Taira
Kazunari
K
Kondo
Yoshinori
Y
D001665:Binding Sites; D008670:Metals / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009843:Oligoribonucleotides / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry*; D012996:Solutions
D008670:Metals; D009843:Oligoribonucleotides; D016337:RNA, Catalytic; D012996:Solutions; C427204:hammerhead ribozyme