CpG oligodeoxynucleotides allow for effective adoptive T-cell therapy in chronic retroviral infection.
Adoptive T-cell therapy in cancer or chronic viral infections is often impeded by the development of functional impairment of the transferred cells. To overcome this therapeutic limitation we combined adoptive transfer of naive, virus-specific CD8+ T cells with immunostimulative CpG oligodeoxynucleotides (ODNs) in mice chronically infected with the Friend retrovirus. The CpG-ODN co-injection prevented the T cells from developing functional defects in IFNgamma and granzyme production and degranulation of cytotoxic molecules. Thus, the transferred T cells were able to reduce chronic viral loads when combined with CpG-ODNs. This strategy provides a new approach for developing successful adoptive T-cell therapy against chronic infections.
Kraft
Anke R M
AR
Institute for Virology, University of Duisburg-Essen, Essen, Germany.
Krux
Frank
F
Schimmer
Simone
S
Ohlen
Claes
C
Greenberg
Philip D
PD
Dittmer
Ulf
U
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000201:enzymology / Q000276:immunology* / Q000502:physiology; D002908:Chronic Disease; D005089:Exocytosis; D005622:Friend murine leukemia virus; D053804:Granzymes / Q000096:biosynthesis; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007371:Interferon-gamma / Q000096:biosynthesis; D007942:Leukemia, Experimental / Q000276:immunology / Q000628:therapy; D051379:Mice; D008822:Mice, Transgenic; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D012192:Retroviridae Infections / Q000276:immunology* / Q000628:therapy*; D014412:Tumor Virus Infections / Q000276:immunology / Q000628:therapy
C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D007371:Interferon-gamma; D053804:Granzymes