[Obtaining of bacterial bioluminescent strain Protobacterium phosphoreum B7071 (lux+) for the determination of zinc ion concentration].
The transconjugate containing hybrid plasmid (Te(R)Zn(R)lux+) was obtained using the conjugation method on Pseudomonasfragi T2(5) (Te(R)ZnR) strain and bioluminescent strain Protobacterium phosphoreum B7071 (lux+). The expression regulation of lux-genes on the obtained plasmid is carried out by promotor-operational area conjointly with zinc resistance genes. The cells of the obtained genetically modified bacteria have the ability to specific induced luminescence, which is a respond to zinc ions' presence in the measuring medium. It was shown that the cells' bioluminescence intensity of trans-conjugate is linearly dependent on zinc ions' concentration within the range of 1-100 microM, that provides the opportunity of using biosensor as a strain for qualitative and quantitative detection of the metal. The low sensitivity limit of this method is 0.5 microM for the metal. Besides having high sensitivity, the developed lux-biosensor is highly specified.
Gruzina
T G
TG
Dybkova
S N
SN
Chekhovskaia
T P
TP
Vember
V V
VV
Zadorozhniaia
A M
AM
Ul'berg
Z R
ZR
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015374:Biosensing Techniques; D002412:Cations / Q000032:analysis; D015964:Gene Expression Regulation, Bacterial; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D010776:Photobacterium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010957:Plasmids; D016133:Polymerase Chain Reaction; D044206:Pseudomonas fragi / Q000235:genetics; D015032:Zinc / Q000032:analysis*
D001426:Bacterial Proteins; D002412:Cations; D008164:Luminescent Proteins; D008156:Luciferases; D015032:Zinc