Quinone-induced inhibition of urease: elucidation of its mechanisms by probing thiol groups of the enzyme.
In this work we studied the reaction of four quinones, 1,4-benzoquinone (1,4-BQ), 2,5-dimethyl-1,4-benzoquinone (2,5-DM-1,4-BQ), tetrachloro-1,4-benzoquinone (TC-1,4-BQ) and 1,4-naphthoquinone (1,4-NQ) with jack bean urease in phosphate buffer, pH 7.8. The enzyme was allowed to react with different concentrations of the quinones during different incubation times in aerobic conditions. Upon incubation the samples had their residual activities assayed and their thiol content titrated. The titration carried out with use of 5,5'-di-thiobis(2-nitrobenzoic) acid was done to examine the involvement of urease thiol groups in the quinone-induced inhibition. The quinones under investigation showed two distinct patterns of behaviour, one by 1,4-BQ, 2,5-DM-1,4-BQ and TC-1,4-BQ, and the other by 1,4-NQ. The former consisted of a concentration-dependent inactivation of urease where the enzyme-inhibitor equilibrium was achieved in no longer than 10min, and of the residual activity of the enzyme being linearly correlated with the number of modified thiols in urease. We concluded that arylation of the thiols in urease by these quinones resulting in conformational changes in the enzyme molecule is responsible for the inhibition. The other pattern of behaviour observed for 1,4-NQ consisted of time- and concentration-dependent inactivation of urease with a nonlinear residual activity-modified thiols dependence. This suggests that in 1,4-NQ inhibition, in addition to the arylation of thiols, operative are other reactions, most likely oxidations of thiols provoked by 1,4-NQ-catalyzed redox cycling. In terms of the inhibitory strength, the quinones studied formed a series: 1,4-NQ approximately 2,5-DM-1,4-BQ<1,4-BQ<TC-1,4-BQ.
Zaborska
Wiesława
W
Jagiellonian University, Faculty of Chemistry, 30-060 Kraków, Ingardena 3, Poland.
Krajewska
Barbara
B
Kot
Mirosława
M
Karcz
Waldemar
W
D016227:Benzoquinones / Q000737:chemistry* / Q000494:pharmacology; D002703:Chloranil / Q000737:chemistry* / Q000494:pharmacology; D053138:Cyclohexenes / Q000737:chemistry* / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D015394:Molecular Structure; D009285:Naphthoquinones / Q000737:chemistry* / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013997:Time Factors; D014510:Urease / Q000037:antagonists & inhibitors / Q000737:chemistry*
D016227:Benzoquinones; D053138:Cyclohexenes; D009285:Naphthoquinones; D013438:Sulfhydryl Compounds; D002703:Chloranil; C020617:2,5-dimethyl-4-benzoquinone; C004532:quinone; D014510:Urease; C035342:1,4-naphthoquinone