Positive correlation between pigment epithelium-derived factor and monocyte chemoattractant protein-1 levels in the aqueous humour of patients with uveitis.
OBJECTIVE
To evaluate whether aqueous humour levels of pigment epithelium-derived factor (PEDF) are associated with monocyte chemoattractant protein-1 (MCP-1) in patients with uveitis.


METHODS
Aqueous humour levels of MCP-1 and PEDF were determined by ELISA in 34 uveitis samples and 9 cataract control samples.


RESULTS
Aqueous humour MCP-1 and PEDF levels were significantly higher in patients with infectious or non-infectious uveitis than in controls (mean (SD) 32.3 (10.7) ng/ml vs 4.48 (1.10) ng/ml vs 0.47 (0.10) ng/ml, and 8.40 (1.30) microg/ml vs 5.01 (0.92) microg/ml vs 1.32 (0.22) microg/ml, respectively, p<0.001). A positive correlation between PEDF and MCP-1 was found in patients with uveitis (r = 0.39, p<0.01).


CONCLUSIONS
The results demonstrated that aqueous humour levels of PEDF were positively associated with MCP-1 in patients with uveitis. The present observations suggest that aqueous humour levels of PEDF may be a marker of inflammation in uveitis.
Yoshida
Yumiko
Y
Department of Medicine, Division of Cardiovascular Medicine, Kurume University School of Medicine, Kurume 830-0011, Japan.
Yamagishi
Sho-Ichi
S
Matsui
Takanori
T
Nakamura
Kazuo
K
Imaizumi
Tsutomu
T
Yoshimura
Koichi
K
Yamakawa
Ryoji
R
D000368:Aged; D001082:Aqueous Humor / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D002386:Cataract / Q000378:metabolism; D018932:Chemokine CCL2 / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015817:Eye Infections / Q000378:metabolism; D005136:Eye Proteins / Q000032:analysis*; D006801:Humans; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000032:analysis*; D015843:Serpins / Q000032:analysis*; D014605:Uveitis / Q000378:metabolism*; D000099174:Pigment Epithelium-Derived Factor
D015415:Biomarkers; D018932:Chemokine CCL2; D005136:Eye Proteins; D009414:Nerve Growth Factors; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor