Pigments from Uroleucon nigrotuberculatum induce apoptosis in HL60 human leukemia cells, implicating intracellular oxidative stress and activation of caspases.
Two pigmented compounds from Uroleucon nigrotuberculatum, uroleuconaphin-B(1) [corrected] (H427) and uroleuconaphin-A(1) [corrected] (H373), significantly diminished the cell viability of HL60 cells with IC50 of 10 microM and 30 microM, respectively, in an 18 h-dye uptake assay. Both H427 and H373 augmented the levels of intracellular reactive oxygen species (ROS) and induced apoptosis as demonstrated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis. ROS augmentation by both H427 and H373 was inhibited by N-acetylcysteine (NAC) and alpha-tocopherol. The apoptosis induced by H427 was inhibited efficiently with NAC and caspase-8 inhibitor but less efficiently with alpha-tocopherol and caspase-9 inhibitor. These findings suggested that these pigments have pro-apoptotic activities via oxidative stress.
Suzuki
Shinya
S
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan. suzukis@ph.bunri-u.ac.jp
Tomita
Mariko
M
Hyodo
Masumi
M
Horikawa
Mitsuyo
M
Tsunoda
Tetsuto
T
Sato
Masao
M
D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D001042:Aphids / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism*; D004789:Enzyme Activation; D018922:HL-60 Cells; D006801:Humans; D020287:In Situ Nick-End Labeling; D018384:Oxidative Stress; D010860:Pigments, Biological / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology
D010860:Pigments, Biological; D020169:Caspases; D024502:alpha-Tocopherol; D000111:Acetylcysteine