Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element.
The technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we were able to successfully transform Aedes fluviatilis (Lutz), which can be used as an avian malaria model. These results demonstrate the usefulness of the piggyBac transposable element as a transformation vector for Neotropical mosquito species and opens up new research frontiers for South American mosquito vectors.
Rodrigues
Flávia Guimarães
FG
Laboratório de Malária, Centro de Pesquisas René Rachou, Av. Augusto de Lima 1715, Fiocruz, 30190-002 Belo Horizonte, MG, Brazil.
Oliveira
Sabrina Barbosa
SB
Rocha
Bruno Coelho
BC
Moreira
Luciano Andrade
LA
D000330:Aedes / Q000235:genetics*; D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D005260:Female; D018014:Gene Transfer Techniques; D017344:Genes, Insect; D005854:Germ Cells; D007303:Insect Vectors / Q000235:genetics*; D008297:Male; D008845:Microinjections; D014170:Transformation, Genetic / Q000235:genetics*
D004251:DNA Transposable Elements