Agrobacterium rhizogenes transformation of the Phaseolus spp.: a tool for functional genomics.
A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes was developed for Phaseolus vulgaris L. wild accessions, landraces, and cultivars and for three other species belonging to the genus Phaseolus: P. coccineus, P. lunatus, and P. acutifolius. Induced hairy roots are robust and grow quickly. The transformation frequency is between 75 and 90% based on the 35-S promoter-driven green fluorescent protein and beta-glucuronidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal determinate nodules that fix nitrogen as efficiently as inoculated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiology, root metabolism, and root-microbe interactions.
Estrada-Navarrete
Georgina
G
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62271, México.
Alvarado-Affantranger
Xochitl
X
Olivares
Juan-Elías
JE
Díaz-Camino
Claudia
C
Santana
Olivia
O
Murillo
Enrique
E
Guillén
Gabriel
G
Sánchez-Guevara
Nayeli
N
Acosta
Jorge
J
Quinto
Carmen
C
Li
Dongxue
D
Gresshoff
Peter M
PM
Sánchez
Federico
F
D015139:Blotting, Southern; D023281:Genomics / Q000379:methods*; D005966:Glucuronidase / Q000032:analysis; D049452:Green Fluorescent Proteins / Q000032:analysis; D009586:Nitrogen Fixation; D027805:Phaseolus / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000033:anatomy & histology / Q000378:metabolism / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics*; D042162:Rhizobium tropici / Q000502:physiology; D014170:Transformation, Genetic
D049452:Green Fluorescent Proteins; D005966:Glucuronidase