Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene.
We studied the transcriptional regulation of the human mitochondrial translation initiation factor 2 (IF2mt) gene. The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes. Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2alpha/beta binds to the IF2mt promoter. Reporter assays, where HEK293T cells were co-transfected with an NRF-2alpha/beta-expressing vector and/or an IF2mt promoter reporter vector, revealed that NRF-2 trans-activates the IF2mt promoter. NRF-2 sites were also found in the promoters of several other mitochondrial translation factors, which suggests NRF-2 may play a key role in the regulation of mitochondrial protein synthesis.
Hayashi
Rippei
R
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Building FSB-401, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture 277-8562, Japan.
Ueda
Takuya
T
Farwell
Mary A
MA
Takeuchi
Nono
N
D002460:Cell Line; D017931:DNA Primers; D051268:GA-Binding Protein Transcription Factor / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D007668:Kidney; D008969:Molecular Sequence Data; D010957:Plasmids; D039881:Prokaryotic Initiation Factor-2 / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
D017931:DNA Primers; D051268:GA-Binding Protein Transcription Factor; D039881:Prokaryotic Initiation Factor-2