Development of fluorescence-labeling method for methylcytosine with metal complexation.
We report a methylcytosine-selective fluorescence labeling. An amino-modified bipyridine was prepared and used as an osmium ligand for methylcytosine-selective oxidation. Different fluorophores were easily incorporated to the modified methylcytosine site through an amide formation of the ligand amino group after osmium complexation.
Tanaka
Kazuo
K
Frontier Research System, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Tainaka
Kazuki
K
Kamei
Taku
T
Okamoto
Akimitsu
A
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D044503:5-Methylcytosine / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry
D005456:Fluorescent Dyes; C522084:N-(4-aminobutyl)-5-(2-(4-methylpyridin-2-yl)pyridin-4-yl)pentanamide; D009838:Oligodeoxyribonucleotides; D015082:2,2'-Dipyridyl; D044503:5-Methylcytosine