Quantitative determination of phyllanthin and hypophyllanthin in Phyllanthus species by high-performance thin layer chromatography.
A simple, precise and rapid high-performance thin-layer chromatographic method has been developed for the estimation of phyllanthin (1) and hypophyllanthin (2), the important lignans of Phyllanthus species, especially Phyllanthus amarus. Separation of 1 and 2 was carried out on silica gel 60 F254 layers eluted with hexane:acetone:ethyl acetate (74:12:8), and the analytes were visualised through colour development with vanillin in concentrated sulphuric acid and ethanol. Scanning and quantification of spots was performed at 580 nm. Recoveries of 1 and 2 were 98.7 and 97.3%, respectively. The method was validated and the peak purities and limits of detection and quantification were determined.
Tripathi
Arvind K
AK
Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow-226015, India.
Verma
Ram K
RK
Gupta
Anil K
AK
Gupta
Madan M
MM
Khanuja
Suman P S
SP
D002855:Chromatography, Thin Layer / Q000379:methods*; D017705:Lignans / Q000737:chemistry*; D029803:Phyllanthus / Q000737:chemistry*
D017705:Lignans; C512682:hypophyllanthin; C517985:phyllanthin