title: Recombinant hepatitis C virus in experimentally infected chimpanzees.
abstract: Genetic recombination between different strains of Hepatitis C virus (HCV) was investigated in three chimpanzees inoculated experimentally with factor VIII concentrate containing HCV subgenotypes 1a, 1b, 2b and 3a. A 750 bp long fragment from the HCV envelope region was amplified by RT-PCR and quasispecies were isolated by plasmid cloning. Nucleotide sequences derived from isolated quasispecies were screened for the presence of inter-subgenotypic recombination by using sequence analysis. Recombination between HCV subgenotype 1a and 1b was found in two animals; each recombinant variant differed by location of predicted crossover region or order of subgenotype 1a and 1b sequences.
authors.affiliation: Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), 1600 Clifton Road NE, Atlanta, GA 30333, USA.
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Gao
authors.affiliation: Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), 1600 Clifton Road NE, Atlanta, GA 30333, USA.
authors.forename: Omana V
authors.initials: OV
authors.lastname: Nainan
authors.affiliation: Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), 1600 Clifton Road NE, Atlanta, GA 30333, USA.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Khudyakov
authors.affiliation: Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), 1600 Clifton Road NE, Atlanta, GA 30333, USA.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Li
authors.affiliation: Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), 1600 Clifton Road NE, Atlanta, GA 30333, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), 1600 Clifton Road NE, Atlanta, GA 30333, USA.
authors.forename: Aileen Co
authors.initials: AC
authors.lastname: Gonzales
authors.affiliation: Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), 1600 Clifton Road NE, Atlanta, GA 30333, USA.
authors.forename: John
authors.initials: J
authors.lastname: Spelbring
authors.affiliation: Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), 1600 Clifton Road NE, Atlanta, GA 30333, USA.
authors.forename: Harold S
authors.initials: HS
authors.lastname: Margolis
mesh_terms: D032761:Animal Experimentation; D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D016679:Genome, Viral; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000503:physiopathology* / Q000635:transmission / Q000821:virology; D002679:Pan troglodytes; D010802:Phylogeny; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid
chemical_list: D004279:DNA, Viral