[Expression profile of nasal NK/T cell lymphoma by cDNA microarray].
OBJECTIVE
To investigate the changes of gene expression profile in nasal NK/T cell lymphoma.


METHODS
Total RNA was extracted from the fresh nasal NK/T cell lymphoma tissue and normal lymph node. Fluorescent labeled cDNA was obtained through synthesizing process by reverse transcription. After hybridization in the two identical microarrays consisting of 4096 genes, overexpressed or underexpressed tumor related genes were analyzed.


RESULTS
In both experimental group and control group, there were six samples. A total of 365 (8.9%) genes was found to be differentially expressed by a factor of twofold or greater in both of two identical cDNA microarrays, which included oncogenes, tumor supressor genes, cell cycle regulators, apoptotic and antiapoptotic factors, DNA transcription factors, DNA repair and recombination factors, signal transduction genes, protein translation genes, as well as a large number of metabolic genes. Thirty-seven of these genes were found to be differentially expressed by a factor of fourfold or greater. The biochemical functions of these differentially expressed genes were diverse.


CONCLUSIONS
This study demonstrates that many different kinds of genes are possibly involved in the initiation and progression of nasal NK/T lymphoma. cDNA microarray technique is useful in screening cancer gene expression for nasal NK/T lymphoma.
Wang
Wei-Hu
WH
Department of Radiation Oncology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China.
Li
Ye-Xiong
YX
Lin
Chen
C
Liu
Xin-Fan
XF
Yu
Zi-Hao
ZH
Song
Yong-Wen
YW
Zhang
Xue-Yan
XY
Fu
Ming
M
Jin
Jing
J
Wang
Shu-Lian
SL
Liu
Yue-Ping
YP
D018951:Antigens, CD20 / Q000235:genetics; D054370:Chemokine CXCL9 / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism* / Q000473:pathology; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000473:pathology; D009669:Nose Neoplasms / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011971:Receptors, Immunologic / Q000235:genetics; D055607:Receptors, Natural Killer Cell
D018951:Antigens, CD20; C080810:CXCL9 protein, human; D054370:Chemokine CXCL9; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell