Recovery of Moraxella ovis from the bovine respiratory tract and differentiation of Moraxella species by tDNA-intergenic spacer PCR.
The purpose of the present study was to identify Moraxella (M.)--like organisms recovered from calves suffering from respiratory disease down to species level by means of tDNA-intergenic spacer length polymorphism analysis (tDNA-PCR), and to perform antimicrobial susceptibility testing of these isolates using an agar dilution technique. A total of 16 isolates originating from 12 unrelated occasions were identified as Moraxella ovis, and tDNA fingerprinting showed clear delineation from other Moraxella species. The minimal inhibitory concentrations (in microg/mL) for 90% of the investigated isolates were < or =0.03 for ampicillin; 0.25 for ceftiofur; 0.5 for oxytetracycline; 8 for gentamicin; 64 for spectinomycin; 0.5/9.5 for the combination trimethoprim-sulfonamides; 4 for erythromycin; 8 for tilmicosin; 1 for florfenicol and 0.125 for enrofloxacin.
Catry
Boudewijn
B
Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium. boudewijn.catry@ugent.be
Boyen
Filip
F
Baele
Margo
M
Dewulf
Jeroen
J
de Kruif
Aart
A
Vaneechoutte
Mario
M
Haesebrouck
Freddy
F
Decostere
Annemie
A
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D017931:DNA Primers / Q000737:chemistry; D021901:DNA, Intergenic / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D009016:Moraxella / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D045828:Moraxellaceae Infections / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011110:Polymorphism, Genetic / Q000235:genetics; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary*; D013045:Species Specificity
D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D017931:DNA Primers; D021901:DNA, Intergenic