Analysis of DNA-bound advanced glycation end-products by LC and mass spectrometry.
Sugars and sugar degradation products readily react in vitro with guanine derivatives, resulting in the formation of DNA-bound advanced glycation end-products (DNA-AGEs). The two diastereomers of N(2)-(1-carboxyethyl)-2'-deoxyguanosine (CEdG(A,B)) and the cyclic adduct of methylglyoxal and 2'-deoxyguanosine (mdG) (N(2)-7-bis(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine have also been detected in cultured cells and/or in vivo. LC-MS/MS methods have been developed to analyze sensitively DNA adducts in vitro and in vivo. In this paper, the chemical structures of possible DNA-AGEs and the application of LC-MS/MS to measure DNA-AGEs are reviewed.
Bidmon
Clemens
C
Institute of Pharmacy and Food Chemistry, University of Erlangen-Nuremberg, Erlangen, Germany.
Frischmann
Matthias
M
Pischetsrieder
Monika
M
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D018736:DNA Adducts / Q000032:analysis; D017127:Glycation End Products, Advanced / Q000032:analysis* / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*
D018736:DNA Adducts; D017127:Glycation End Products, Advanced; D004247:DNA