title: Relationship between total salivary protein content and volatile sulfur compounds levels in malodor patients.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the relationship between total salivary protein and VSC levels.
  
  
  METHODS
  A patient group comprised 67 patients from the Fresh Breath Clinic of the Dental Hospital, Tokyo Medical and Dental University, with 18 healthy subjects as a control group. Gas chromatography was used to measure concentrations of VSCs in mouth air. After collecting resting saliva, flow rate, pH, and the amount of total protein in saliva were measured. Salivary protein patterns were analyzed by polyacrylamide gel electrophoresis (PAGE).
  
  
  RESULTS
  In the patient group, the amounts of total salivary protein were significantly correlated with CH3SH levels measured by GC, CH3SH/H2S ratio, and organoleptic score. In addition, a significant correlation between flow rate of salivary protein and CH3SH/H2S ratio and the higher levels of salivary proteins from PAGE analyses were found.
  
  
  CONCLUSIONS
  Total salivary protein was considered to be involved in the formation of oral malodor as one of the nutrient sources. The total salivary protein could be changed both quantitatively and qualitatively in subjects with malodor.
authors.affiliation: Department of Oral Health Promotion, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan. praweena_s@hotmail.com
authors.forename: Praweena
authors.initials: P
authors.lastname: Sopapornamorn
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ueno
authors.forename: Kayoko
authors.initials: K
authors.lastname: Shinada
authors.forename: Masaki
authors.initials: M
authors.lastname: Yanagishita
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kawaguchi
mesh_terms: D000328:Adult; D001944:Breath Tests; D016022:Case-Control Studies; D002849:Chromatography, Gas; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006209:Halitosis / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000473:pathology; D012463:Saliva / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012636:Secretory Rate; D013457:Sulfur Compounds / Q000032:analysis*; D014059:Tongue / Q000382:microbiology / Q000473:pathology
chemical_list: D012471:Salivary Proteins and Peptides; D013457:Sulfur Compounds