title: Glucocorticoid receptor subunit gene expression in parotid gland and adenomas.
abstract: OBJECTIVE
  The present study was undertaken to investigate the expressions of glucocorticoid receptor-alpha (GR-alpha) and -beta (GR-beta) messenger RNA (mRNA) in normal parotid and adenoma tissues.
  
  
  METHODS
  Eighteen pleomorphic adenomas of the parotid gland and 12 parotid gland tissues adjacent to adenomas were studied by using real-time fluorescent quantitative reverse-transcription polymerase chain reaction method.
  
  
  RESULTS
  The expression of both GR-alpha and GR-beta mRNA in parotid adenoma were higher than that in normal parotid glands (P<0.001), the GR-alpha/GR-beta ratios in parotid adenoma were lower than that in normal parotid glands (P<0.001), and there were no differences of both GR-alpha and GR-beta mRNA as well as GR-alpha/GR-beta ratios in male and female (P>0.05).
  
  
  CONCLUSIONS
  Our results showed that the mRNA expression of both GR-alpha and GR-beta were detectable in all studied specimens. The mRNA levels of these 2 GRs were higher, whereas the GR-alpha/GR-beta ratios were lower in adenomas tissues than that in the parotid gland; no differences of these 2 GRs as well as GR-alpha/GR-beta ratios were found between sexes. These data indicate that the relationship between the expressions of GRs and the clinical significance in parotid adenomas need further and profound investigation.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated Hospital, Sun Yat-Sen University, Guangdong, PR China. zyzdd7755@sina.com.cn
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Jia-Jun
authors.initials: JJ
authors.lastname: Liu
authors.forename: Xian
authors.initials: X
authors.lastname: Liu
authors.forename: Zhen-Lin
authors.initials: ZL
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
mesh_terms: D008949:Adenoma, Pleomorphic / Q000235:genetics* / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D010306:Parotid Gland / Q000378:metabolism*; D010307:Parotid Neoplasms / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; C411470:glucocorticoid receptor alpha; C121943:glucocorticoid receptor beta