Comparison of two automated spectrophotometric methods for ceruloplasmin measurement in pigs.
Ceruloplasmin (Cp) determination could provide an objective measure of the health status of an animal and could be used as marker of animal health and welfare (Skinner, 2001) (Skinner, J. G., 2001. Special report. International standardization of acute phase proteins. Veterinary Clinical Pathology 30, 2-7.) but only manual methods have been reported to determine Cp concentrations in pigs (McCosker, 1961; Toussaint et al., 1995; Eckersall et al., 1996) (McCosker, P. J., 1961. Paraphenylenediamine oxidase activity and copper-levels in mammalian plasmas. Nature. 190, 887-889; Toussaint, M. J. M., Van Ederen, A. M., Gruys, E., 1995. Implication of clinical pathology in assessment of animal health and in animal production and meat inspection. Comparative Haematology International 5, 149-157; Eckersall, P. D., Saini, P. K., McComb, C., 1996. The acute phase response of acid soluble glycoprotein, alpha(1)-acid glycoprotein, ceruloplasmin, haptoglobin and C-reactive protein, in the pig. Veterinary Immunology and Immunopathology 51, 377-385). In the present study two automated methods based on the use of two different substrates for the determination of serum ceruloplasmin in pigs were developed, evaluated and compared. Both methods showed a good precision and detection limits, with no signs of inaccuracy and could be applied to biochemical autoanalyzers usually found in clinical laboratories using only minimal amounts of serum. Additionally the behaviour of Cp in experimental and clinical situations was studied showing an increase of around two fold after turpentine administration and significantly higher values in cases of pigs with inflammatory conditions when compared with healthy pigs.
Martínez-Subiela
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Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, Campus de Espinardo, 30100 Espinardo, Murcia, Spain. jjceron@um.es
Tecles
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Ceron
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D000818:Animals; D001331:Automation; D002570:Ceruloplasmin / Q000032:analysis*; D005260:Female; D006461:Hemolysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008562:Membrane Glycoproteins; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods / Q000662:veterinary*; D013552:Swine / Q000097:blood*
D008562:Membrane Glycoproteins; C048022:substrate adhesion molecules; D002570:Ceruloplasmin