Synthesis of cross-linked triple helical DNAs possessing high affinity to triple helical DNA binding protein.
We examined binding ability of MBP-LOR3(ARF) protein to triplexes composed of branched oligonucleotides possessing a pentaerithritol linker. It was found that the triplex, which has an antraquinonecarbonyl group at the 5'-end of the third strand and is connected with the pentaerithritol linker, has greater affinity to the MBP-LOR3(ARF) protein than an unmodified triplex.
Shibata
Aya
A
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido, Gifu 501-1193, Japan.
Ueno
Yoshihito
Y
Nakanishi
Masayuki
M
Matsuda
Akira
A
Kitade
Yukio
Y
D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry; D011993:Recombinant Fusion Proteins
D004268:DNA-Binding Proteins; C520206:MBP-LOR3(ARF) protein, human; D009841:Oligonucleotides; D011993:Recombinant Fusion Proteins; C051802:triplex DNA; D004247:DNA