title: Interleukin 2 gene transcription is regulated by Ikaros-induced changes in histone acetylation in anergic T cells.
abstract: In T cells anergy may be evoked by an unbalanced stimulation of the T-cell receptor in the absence of costimulation. Anergic T cells are unresponsive to new antigen receptor engagement and do not produce interleukin 2. We present evidence that anergizing stimuli induce changes in histone acetylation, which mediates transcriptional repression of interleukin 2 expression. In response to calcium signaling, anergic T cells up-regulate the expression of Ikaros, a zinc finger transcription factor essential for lymphoid lineage determination. Ikaros binds to the interleukin 2 promoter where it induces histone deacetylation. Confirming the role of Ikaros in the induction of T-cell anergy, cells with reduced Ikaros activity show defective inactivation in response to an anergizing stimulus. We propose a model in which tolerizing stimuli induce epigenetic changes on the interleukin 2 locus that are responsible for the stable inhibition of the expression of this cytokine in anergic T cells.
authors.affiliation: Albert Einstein College of Medicine, Department of Pathology, Bronx, NY 10461, USA.
authors.forename: Sanmay
authors.initials: S
authors.lastname: Bandyopadhyay
authors.forename: Myrianne
authors.initials: M
authors.lastname: Duré
authors.forename: Monika
authors.initials: M
authors.lastname: Paroder
authors.forename: Noemí
authors.initials: N
authors.lastname: Soto-Nieves
authors.forename: Irene
authors.initials: I
authors.lastname: Puga
authors.forename: Fernando
authors.initials: F
authors.lastname: Macián
mesh_terms: D000107:Acetylation; D000818:Animals; D017635:Clonal Anergy / Q000235:genetics* / Q000276:immunology*; D044127:Epigenesis, Genetic; D006657:Histones / Q000378:metabolism*; D006801:Humans; D051740:Ikaros Transcription Factor / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000235:genetics*; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018448:Models, Immunological; D011401:Promoter Regions, Genetic; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D006657:Histones; D007376:Interleukin-2; C497238:Zfpn1a1 protein, mouse; D051740:Ikaros Transcription Factor