title: One-step generation of recombineering constructs by asymmetric-end ligation and negative selection.
authors.affiliation: Division of Basic Molecular Science and Molecular Medicine, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa 259-1193, Japan. masato@is.icc.u-tokai.ac.jp
authors.forename: Masato
authors.initials: M
authors.lastname: Ohtsuka
authors.forename: Akiko
authors.initials: A
authors.lastname: Mizutani
authors.forename: Yukie Y
authors.initials: YY
authors.lastname: Kikuti
authors.forename: Jerzy K
authors.initials: JK
authors.lastname: Kulski
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sato
authors.forename: Minoru
authors.initials: M
authors.lastname: Kimura
authors.forename: Masafumi
authors.initials: M
authors.lastname: Tanaka
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Inoko
mesh_terms: D003001:Cloning, Molecular; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics*
chemical_list: D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins