Cloning and characterization of a cDNA encoding a cowpea seed defensin and analysis of its expression.
We have previously isolated a cowpea seed defensin with potent antifungal activity. Here we report the cloning of its cDNA obtained from RT-PCR. The sequence of the deduced protein VUDEF exhibited homology to plant defensins. Northern analysis revealed that VUDEF mRNA is accumulated during cowpea seed development and its level increases in seedling tissues after exposure to fungal pathogen and cold.
Carvalho
André O
AO
Laboratório de Fisiologia e Bioquímica de Microrganismos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes - RJ, Brazil.
Filho
Gonçalo A S
GA
Ferreira
Beatriz S
BS
Branco
Alan T
AT
Okorokova-Façanha
Anna L
AL
Gomes
Valdirene M
VM
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D023082:Defensins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D007887:Fabaceae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012639:Seeds / Q000235:genetics* / Q000378:metabolism*
D018076:DNA, Complementary; D023082:Defensins