RNA isolation using immobilized PNA.
A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.
Ohtsuki
Takashi
T
Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
Kumano
Chisato
C
Manabe
Taishi
T
Kitamatsu
Mizuki
M
Sisido
Masahiko
M
D001483:Base Sequence; D019921:Biotinylation; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012329:RNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D012343:RNA, Transfer / Q000302:isolation & purification*; D012356:RNA, Transfer, Leu / Q000737:chemistry / Q000302:isolation & purification
D020135:Peptide Nucleic Acids; D012329:RNA, Bacterial; D012356:RNA, Transfer, Leu; D012343:RNA, Transfer