title: Chemical behavior of bis(3'-5')diguanylic acid in aqueous solutions.
abstract: This paper describes unique behavior of bis(3'-5')diguanylic acid (c-di-GMP) under some conditions. Thus, c-di-GMP exists as the monomer in aprotic organic solvents such as DMSO. By contrast, c-di-GMP smoothly aggregates in water and in low-concentration aqueous solutions of some salts, such as sodium chloride and ammonium acetate, to give a mixture of many aggregates. The resulting multiple aggregates converge to the single compound (provably the monomer) in a >154 mM (0.9%) sodium chloride aqueous solution, in a >100 mM ammonium acetate buffer, and in a >100 mM phosphate buffer.
authors.affiliation: Graduate School of Information Science/Human Informatics, and CREST/JST, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hyodo
authors.forename: Yumi
authors.initials: Y
authors.lastname: Sato
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hayakawa
authors.forename: David K R
authors.initials: DK
authors.lastname: Karaolis
mesh_terms: D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D012996:Solutions; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D002021:Buffers; D012996:Solutions; D012997:Solvents; D014867:Water; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP