Exploration of human miRNA target genes in neuronal differentiation.
MicroRNAs (miRNAs) are endogenous non-coding RNA molecules that inhibit protein translation in a sequence specific manner. We carried out microarray analyses of 180 human pre-miRNAs in neuroblastoma cell, SH-SY5Y, following stimulation by TPA. Twelve of the pre-miRNAs were up-regulated by the TPA stimulation. We also explored miRNA target genes associated with neuronal differentiation. Some miRNAs have complementarity with 3'UTR of the Notch1 gene, a regulator of neuronal differentiation, and luciferase assay showed that overexpression of these miRNAs reduced the luciferase activity of reporter genes containing the Notch1-3'UTR sequence. Our results suggest that miRNAs can be associated with TPA induced signalling pathways and expression of Notch1 gene.
Fukuda
Yoko
Y
Department of Neuroscience, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Kawasaki
Hiroaki
H
Taira
Kazunari
K
D002454:Cell Differentiation; D002460:Cell Line; D020869:Gene Expression Profiling; D017930:Genes, Reporter; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012322:RNA Precursors / Q000378:metabolism; D051881:Receptor, Notch1 / Q000235:genetics
D035683:MicroRNAs; D012322:RNA Precursors; D051881:Receptor, Notch1