Diacyltrehalose of Mycobacterium tuberculosis inhibits lipopolysaccharide- and mycobacteria-induced proinflammatory cytokine production in human monocytic cells.
The lipids located in the outer layer of Mycobacterium tuberculosis, which include sulfolipid, phthiocerol dimycocerosate (PDIM), diacyltrehalose, and polyacyltrehalose, may play a role in host-pathogen interactions. These lipids were purified using thin-layer chromatography, and their ability to induce proinflammatory cytokines in human monocytes and in a human acute monocytic leukemia cell line (THP-1) was examined. None of the lipids tested induced significant interleukin (IL)-12p40 or tumor necrosis factor (TNF)-alpha production in monocytic cells. Diacyltrehalose significantly inhibited lipopolysaccharide- and M. tuberculosis-induced IL-12p40, TNF-alpha, and IL-6 productions in human monocytes, whereas other lipids had no effect. However, diacyltrehalose was unable to inhibit peptidoglycan-induced IL-12p40 production. These results suggest that diacyltrehalose is a mycobacterial factor capable of modulating host immune responses.
Lee
Kil-Soo
KS
Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Korea.
Dubey
Vinod S
VS
Kolattukudy
Pappachan E
PE
Song
Chang-Hwa
CH
Shin
A-Rum
AR
Jung
Saet-Byel
SB
Yang
Chul-Su
CS
Kim
Su-Young
SY
Jo
Eun-Kyeong
EK
Park
Jeong-Kyu
JK
Kim
Hwa-Jung
HJ
D045744:Cell Line, Tumor; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D053762:Interleukin-12 Subunit p40 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008055:Lipids / Q000276:immunology* / Q000302:isolation & purification; D009000:Monocytes / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000276:immunology*; D014199:Trehalose / Q000031:analogs & derivatives* / Q000276:immunology* / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016207:Cytokines; D053762:Interleukin-12 Subunit p40; D015850:Interleukin-6; D008055:Lipids; D014409:Tumor Necrosis Factor-alpha; D014199:Trehalose