title: Mutagenesis by damaged deoxyribonucleotides and its prevention by MutT-type hydrolyzing enzymes.
abstract: Oxidized deoxyribonucleotides, 2-hydroxydeoxyadenosine 5'-triphosphate (2-OH-dATP) and 8-hydroxydeoxyguanosine 5'-triphosphate (8-OH-dGTP), were introduced into Escherichia coli strains deficient in DNA polymerase IV (a Y-family DNA polymerase encoded in the dinB gene), and the MutT and Orfl35 proteins to examine their in vivo roles in mutagenesis elicited by 2-OH-dATP and 8-OH-dGTP. 2-OH-dATP elicited mutations less efficiently in the dinB- strain than in the wild type strain, suggesting involvement of DNA polymerase IV in 2-OH-dATP-induced mutations. 8-OH-dGTP and 2-OH-dATP elicited mutations more efficiently in mutT- and orfl35- strains, respectively, than those in their isogenic mutT+ and orfl35+ strains. These results indicate that these proteins play important roles in mutagenesis induced by 2-OH-dATP and 8-OH-dGTP in vivo.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamiya
authors.forename: Kazuya
authors.initials: K
authors.lastname: Satou
authors.forename: Mika
authors.initials: M
authors.lastname: Hori
authors.forename: Emiko
authors.initials: E
authors.lastname: Iida
authors.forename: Chieko
authors.initials: C
authors.lastname: Ishiguro
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D004249:DNA Damage; D019951:DNA Polymerase beta / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006868:Hydrolysis; D016296:Mutagenesis; D011755:Pyrophosphatases / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D019951:DNA Polymerase beta; D011755:Pyrophosphatases; C052211:mutT protein, E coli