title: Autoradiographic localization of aromatic hydrocarbon receptor (AHR) in rhesus monkey ovary.
abstract: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most toxic congener of a large class of manmade pollutants that persist in the environment. TCDD exerts its toxic effects, in part, by binding to its receptor known as the aromatic hydrocarbon receptor (AHR). TCDD is estrogen modulatory and in some systems its receptor associates directly with estrogen receptors via co-activator molecules. TCDD inhibits steroid synthesis in human ovarian granulosa cells and AHR is found in these cells. We have previously shown that AHR is found in whole rhesus monkey ovary, but have yet to establish its location. In the present study, we set out to show that radiolabeled TCDD binds to monkey ovarian follicles and that this binding is receptor mediated. Ovaries from Macaca mulatta were sectioned on a cryostat at 10 micro m; and sections were incubated with either control vehicle, (3)H-TCDD, or (3)H-TCDD plus alpha-naphthoflavone (ANF), a known receptor-blocking agent. Here, we show for the first time specific binding of TCDD to the granulosa cells of antral follicles and other regions of the rhesus monkey ovary. Our data indicate a 60-fold increase in binding with (3)H-TCDD over that of control, and that this binding is reduced to the levels seen in controls with the addition of the competitive antagonist ANF. These findings support the hypothesis that TCDD directly affects primate ovarian function via the AHR.
authors.affiliation: Division of Natural and Health Sciences, Carroll College, Waukesha, Wisconsin 53186, USA. mbaldrid@cc.edu
authors.forename: Monika G
authors.initials: MG
authors.lastname: Baldridge
authors.forename: Reinhold J
authors.initials: RJ
authors.lastname: Hutz
mesh_terms: D000818:Animals; D001345:Autoradiography; D001571:Benzoflavones / Q000378:metabolism; D016616:Cryoultramicrotomy; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D016014:Linear Models; D008253:Macaca mulatta / Q000378:metabolism*; D000072317:Polychlorinated Dibenzodioxins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*; D014316:Tritium
chemical_list: D001571:Benzoflavones; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D014316:Tritium; C011512:alpha-naphthoflavone