title: Molecular epidemiology of adenoviral conjunctivitis in Hanoi, Vietnam.
abstract: OBJECTIVE
  To investigate the serotypes of adenovirus causing conjunctivitis in Hanoi, Vietnam.
  
  
  METHODS
  Clinical diagnosis of adenoviral conjunctivitis and laboratory-based experimental study.
  
  
  METHODS
  We collected 21 conjunctival swabs from 21 different patients with a clinical presentation compatible with adenoviral conjunctivitis, in Hanoi, Vietnam. Immunochromatography and real-time polymerase chain reaction (PCR) were performed to detect human adenovirus (HAdV). The sequence of PCR products was analyzed to determine the serotype of HAdV.
  
  
  RESULTS
  Of 21 samples, HAdV DNA was detected in 14 samples (66.7%) by real-time PCR. The serotype analysis showed HAdV-8 in 11 samples (78.6%), HAdV-3 in two samples (14.3%), and HAdV-37 in one sample (7.1%). Of 11 HAdV-8 samples, one sample (9.1%) was prototype, and the other 10 samples (90.9%) had identical nucleotide sequence and were identified as a variant of HAdV-8.
  
  
  CONCLUSIONS
  HAdV-8 was found to be the predominant serotype in Hanoi, Vietnam. Most of the HAdV-8 samples were a variant of HAdV-8.
authors.affiliation: Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo, Japan. xhjin@med.hokudai.ac.jp
authors.forename: Xue-Hai
authors.initials: XH
authors.lastname: Jin
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishiko
authors.forename: Thanh Ha
authors.initials: TH
authors.lastname: Nguyen
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohguchi
authors.forename: Masataka
authors.initials: M
authors.lastname: Akanuma
authors.forename: Koki
authors.initials: K
authors.lastname: Aoki
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ohno
mesh_terms: D000258:Adenovirus Infections, Human / Q000453:epidemiology* / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics*; D002845:Chromatography; D003228:Conjunctiva / Q000821:virology; D003236:Conjunctivitis, Viral / Q000453:epidemiology* / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D007158:Immunologic Techniques; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D014744:Vietnam / Q000453:epidemiology
chemical_list: D004279:DNA, Viral