Preparation of diverse metal binding sites along the helical structure of duplex DNA.
DNA duplexes containing 4-N-carboxymethylcytosine (1) were synthesized: duplex-I (5'-GTGACCA1TGCAG TG-3':3'-CACTGGT1ACGTCAC-5'), duplex-II (5'-GC CCA1TCCA1TGCG-3':3'-CG GGT1AGGT1ACGC-5'), and duplex-III (5'-GCTCCA11TGCACCG-3':3'-CGAG GT11ACGTGGC-5'). In the presence of Cu(II) or Ni(II) ions, duplex-I and duplex-II, which contain isolated 1-1 pair(s), were stabilized. In contrast, duplex-III, which contained consecutive 1-1 pairs, was stabilized significantly in the presence of Cu(II) ions, while the addition of Ni(II) ions had no notable effect on its thermal denaturation profile.
Sugiyama
Kumiko
K
Department of Material and Life Chemistry, Faculty of Engineering, Kanagawa University, 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama, Kanagawa 221-8686, Japan.
Ono
Akira
A
D020029:Base Pairing; D001665:Binding Sites; D003300:Copper / Q000737:chemistry; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*
C522075:4-N-carboxymethylcytosine; D008670:Metals; D009838:Oligodeoxyribonucleotides; D003300:Copper; D009532:Nickel; D003596:Cytosine; D004247:DNA