title: Multiplex formation of repetitive sequences d([G]nA)m through non-WC pairings: crystal structure of d(gcGAGGGAgc).
abstract: The crystal structure of a DNA fragment with the sequence d(gcGA[G](3)Agc), a shortened and mutated derivative of the VNTR sequence d(ccGA[G](4)Agg), has been determined by X-ray analyses at 1.8 A resolution to investigate structures and mechanisms of a non-coding DNA. This sequence forms an antiparallel duplex with two WC pairings at the both ends and six consecutive non-WC pairings in the major part. Based on this structure, d(GA[G](n)A) (n=3-6) and d([G](n)A)(m) (n>1 and m>1) were designed. It is expected that all bases form non-WC pairs when the DNA fragments adapt antiparallel duplex. Electrophoresis experiments showed that these DNA fragments form not only duplexes, but also multiplexes such as quadruplexes, octaplexes, and so on.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Sato
authors.forename: Kenta
authors.initials: K
authors.lastname: Mitomi
authors.forename: Hiromu
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Satoru
authors.initials: S
authors.lastname: Shimizu
authors.forename: Ella C M
authors.initials: EC
authors.lastname: Juan
authors.forename: Jiro
authors.initials: J
authors.lastname: Kondo
authors.forename: Akio
authors.initials: A
authors.lastname: Takénaka
mesh_terms: D020029:Base Pairing; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D003853:Deoxyribonucleosides / Q000737:chemistry*; D004586:Electrophoresis; D054856:G-Quadruplexes; D018598:Minisatellite Repeats; D008958:Models, Molecular
chemical_list: D003853:Deoxyribonucleosides; C522080:d(gcGAGGGAgc); D004247:DNA