title: Polymorphism at the defensin gene in the Anopheles gambiae complex: testing different selection hypotheses.
abstract: Genetic variation in defensin, a gene encoding a major effector molecule of insects immune response was analyzed within and between populations of three members of the Anopheles gambiae complex. The species selected included the two anthropophilic species, An. gambiae and An. arabiensis and the most zoophilic species of the complex, An. quadriannulatus. The first species was represented by four populations spanning its extreme genetic and geographical ranges, whereas each of the other two species was represented by a single population. We found (i) reduced overall polymorphism in the mature peptide region and in the total coding region, together with specific reductions in rare and moderately frequent mutations (sites) in the coding region compared with non-coding regions, (ii) markedly reduced rate of non-synonymous diversity compared with synonymous variation in the mature peptide and virtually identical mature peptide across the three species, and (iii) increased divergence between species in the mature peptide together with reduced differentiation between populations of An. gambiae in the same DNA region. These patterns suggest a strong purifying selection on the mature peptide and probably the whole coding region. Because An. quadriannulatus is not exposed to human pathogens, identical mature peptide and similar pattern of polymorphism across species implies that human pathogens played no role as selective agents on this peptide.
authors.affiliation: Organisation de Coordination pour la lutte Contre les Endémies en Afrique Centrale (OCEAC), BP 288, Yaoundé, Cameroon. simard@ird.fr
authors.forename: Frédéric
authors.initials: F
authors.lastname: Simard
authors.forename: Monica
authors.initials: M
authors.lastname: Licht
authors.forename: Nora J
authors.initials: NJ
authors.lastname: Besansky
authors.forename: Tovi
authors.initials: T
authors.lastname: Lehmann
mesh_terms: D000349:Africa; D019943:Amino Acid Substitution; D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics*; D023082:Defensins / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D006801:Humans; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012641:Selection, Genetic
chemical_list: D023082:Defensins