title: Quantum dots for human mesenchymal stem cells labeling. A size-dependent autophagy activation.
abstract: Lately certain cytotoxicity of quantum dots (QDs) and some deleterious effects of labeling procedure on stem cells differentiation abilities were shown. In the present study we compared cytotoxicity and intracellular processing of two different-sized protein-conjugated QDs after labeling of the human mesenchymal stem cells (hMSC). An asymmetrical intracellular uptake of red (605 nm) and green (525 nm) quantum dots was observed. We describe for the first time a size-dependent activation of autophagy, caused by nanoparticles.
authors.affiliation: Center for Biotechnology and Biomedicine (BBZ), University of Leipzig, D-04103 Leipzig, Germany. alex_seleverstov@yahoo.de
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Seleverstov
authors.forename: Olga
authors.initials: O
authors.lastname: Zabirnyk
authors.forename: Matthias
authors.initials: M
authors.lastname: Zscharnack
authors.forename: Larysa
authors.initials: L
authors.lastname: Bulavina
authors.forename: Marcin
authors.initials: M
authors.lastname: Nowicki
authors.forename: Jan-Michael
authors.initials: JM
authors.lastname: Heinrich
authors.forename: Maksym
authors.initials: M
authors.lastname: Yezhelyev
authors.forename: Frank
authors.initials: F
authors.lastname: Emmrich
authors.forename: Ruth
authors.initials: R
authors.lastname: O'Regan
authors.forename: Augustinus
authors.initials: A
authors.lastname: Bader
mesh_terms: D001343:Autophagy; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D005453:Fluorescence; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D045663:Quantum Dots
chemical_list: C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins