A novel method for oral delivery of drug compounds to the neonatal SMNDelta7 mouse model of spinal muscular atrophy.
Spinal muscular atrophy (SMA) is a devastating motor neuron disease that is one of the leading genetic causes of infant mortality. Currently, there is no cure for SMA. Mouse models that genetically and phenotypically resemble SMA have been generated and have the potential to be used for the discovery of novel therapeutics. Oral administration is a commonly used mode of drug delivery in humans as well as in rodents. Unfortunately, there is no method of drug delivery that can accurately and reliably deliver drug compounds orally to neonatal mice. In this report, we describe a novel method to orally administer compounds to neonatal SMA mice. Oral delivery to neonatal mice, usually starting at postnatal day 4 (PND04), is both rapid and safe to the pup. Oral delivery of two different commonly used vehicle formulations, distilled water and 2-hydroxypropyl-beta-cyclodextrin, does not affect the survival of SMA mice. After oral delivery for 3 days, 5-bromo-2'-deoxyuridine could be detected in the kidneys, brains and spinal cords of treated non-SMA as well as SMA neonatal pups. In conclusion, we have developed a method by which drugs can be safely and reliably administered orally to neural targets of neonatal mice. This approach offers a simple and rapid means by which potential therapeutics for SMA can be identified.
Butchbach
Matthew E R
ME
Department of Molecular and Cellular Biochemistry, College of Medicine, Ohio State University, 363 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210, USA. butchbach.1@osu.edu
Edwards
Jonathan D
JD
Schussler
Kristie R
KR
Burghes
Arthur H M
AH
D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D009134:Muscular Atrophy, Spinal / Q000378:metabolism*; D009928:Organ Specificity; D013116:Spinal Cord / Q000378:metabolism*; D014018:Tissue Distribution
D001973:Bromodeoxyuridine