IADS, a decomposition product of DIDS activates a cation conductance in Xenopus oocytes and human erythrocytes: new compound for the diagnosis of cystic fibrosis.
DIDS (4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid) is a commonly used blocker of plasma membrane anion channels and transporters. We observed that DIDS undergoes decomposition while stored in DMSO (dimethyl sulfoxide) forming a biologically active compound. One decomposition product, called IADS, was identified and synthesized. Voltage-clamp and patch clamp experiments on Xenopus laevis oocytes and human erythrocytes revealed that IADS is able to activate a plasma membrane cation conductance in both cell types. Furthermore, we found that IADS induces hemolysis in red blood cells of healthy donors but fails to hemolyze erythrocytes of donors with cystic fibrosis. Thus, IADS stimulated activation of a cation conductance could form the basis for a novel diagnostic test of cystic fibrosis.
Stumpf
Astrid
A
Institute of Physiology II, University of Münster, Münster, Germany.
Almaca
Joana
J
Kunzelmann
Karl
K
Wenners-Epping
Kerstin
K
Huber
Stephan M
SM
Haberle
Johannes
J
Falk
Sabine
S
Duebbers
Angelika
A
Walte
Mike
M
Oberleithner
Hans
H
Schillers
Hermann
H
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002412:Cations / Q000378:metabolism; D003550:Cystic Fibrosis / Q000175:diagnosis*; D004594:Electrophysiology; D004912:Erythrocytes / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006461:Hemolysis; D006801:Humans; D017136:Ion Transport / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D014981:Xenopus
C517099:4-isothiocyanato-4'-amino-stilbene-2,2'-disulfonic acid; D002412:Cations; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid