RNA template-directed photoligation via 5-carboxyvinyl-2'-deoxyuridine.
An efficient RNA template-directed photoligation of oligodeoxynucleotides (ODNs) via 5-carboxyvinyl-2'-deoxyuridine ((CV)U) was described. When ODN containing (CV)U at the 5' end was photoirradiated with ODN containing a pyrimidine base at the 3' end in the presence of template RNA, an efficient photoligation was observed. RNA template-directed photoligation of ODNs should provide a useful technique for sensitive and accurate RNA detection.
Yoshimura
Yoshinaga
Y
PRESTO, Japan Science and Technology Agency.
Noguchi
Yuuki
Y
Sato
Hideaki
H
Fujimoto
Kenzo
K
D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D008027:Light; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000528:radiation effects*; D012313:RNA / Q000737:chemistry*; D013698:Templates, Genetic
C520201:5-carboxyvinyl-2'-deoxyuridine; D009838:Oligodeoxyribonucleotides; D012313:RNA; D003857:Deoxyuridine