Deamination of 2',3'-O-isopropylideneadenosine-5'- carboxylic acid catalyzed by adenosine deaminase (ADA) and adenylate deaminase (AMPDA): influence of substrate ionization on the activity of the enzymes.
Adenosine deaminase (ADA) and adenylate deaminase (AMPDA) catalyze the deamination of 2 ',3 '-O-isopropylideneadenosine-5'-carboxylic acid to the corresponding inosine derivative and dependence of the rate of enzymatic reaction on the ionization degree of the substrate has been studied at different pH values.
Ciuffreda
Pierangela
P
Dipartimento di Scienze Precliniche LITA Vialba, Università degli Studi di Milano, Milano, Italy.
Alessandrini
Laura
L
Pavlovic
Radmila
R
Santaniello
Enzo
E
D000659:AMP Deaminase / Q000737:chemistry*; D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry; D000243:Adenosine Deaminase / Q000737:chemistry*; D002384:Catalysis; D003641:Deamination; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D013379:Substrate Specificity
C518810:2',3'-O-isopropylideneadenosine-5'-carboxylic acid; D007477:Ions; D000243:Adenosine Deaminase; D000659:AMP Deaminase; D000241:Adenosine