Paenibacillus cellulosilyticus sp. nov., a cellulolytic and xylanolytic bacterium isolated from the bract phyllosphere of Phoenix dactylifera.
A bacterial strain designated PALXIL08(T) was isolated from the phyllosphere of Phoenix dactylifera bracts. A phylogenetic analysis based on the 16S rRNA gene sequence placed the isolate within the genus Paenibacillus in the same subgroup as Paenibacillus kobensis and Paenibacillus curdlanolyticus (98.9 and 97.9 % sequence similarity, respectively). The DNA hybridization values between the isolate and the type strains of Paenibacillus kobensis and Paenibacillus curdlanolyticus were found to be 27.4 and 17.6 %, respectively. The isolate comprised Gram-variable, facultatively anaerobic, motile, sporulating rods. Catalase and oxidase were produced and cellulose, xylan, starch and aesculin were hydrolysed. Many carbohydrates served as carbon sources for growth. MK-7 was the predominant menaquinone, and anteiso-C(15 : 0) and iso-C(16 : 0) were the major fatty acids. The DNA G+C content was 51 mol%. DNA relatedness data and the results of phylogenetic and phenotypic analyses showed that strain PALXIL08(T) should be considered as the type strain of a novel species of the genus Paenibacillus, for which the name Paenibacillus cellulosilyticus sp. nov. is proposed. The type strain is PALXIL08(T) (=LMG 22232(T)=CECT 5696(T)).
Rivas
Raúl
R
Departamento de Microbiología y Genética, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
García-Fraile
Paula
P
Departamento de Microbiología y Genética, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
Mateos
Pedro F
PF
Departamento de Microbiología y Genética, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
Martínez-Molina
Eustoquio
E
Departamento de Microbiología y Genética, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
Velázquez
Encarna
E
Departamento de Microbiología y Genética, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
D028023:Arecaceae / Q000382:microbiology*; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002482:Cellulose / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014990:Xylans / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014990:Xylans; D002482:Cellulose