[Association study of single nucleotide polymorphism of interleukin 6 receptor gene and type 2 diabetes in Chinese Han population].
OBJECTIVE
To determine the association of the promoter and exon 9 of interleukin 6 receptor gene IL6R and the susceptibility of type 2 diabetes mellitus in Chinese Han population.


METHODS
A total of 183 unrelated subjects, including 87 type 2 diabetes and 96 normal glucose tolerant (NGT) controls, were recruited. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were employed to screen the polymorphism.


RESULTS
In the normal glucose tolerant control group, the genotype frequencies and allele frequencies of D358A were AA 31.2%, AC 41.7%, CC 27.1%, A 52.1%, C 47.9%, while the corresponding frequencies in the type 2 diabetes group were AA 44.8%, AC 41.4%, CC 13.8%, A 65.5%, C 34.5% respectively, the CC genotype frequency and C allele frequency of normal glucose tolerant control was higher than that of type 2 diabetes (Chi2 test=4.900, P< 0.05; Chi2 test=6.784, P< 0.01). No differences were found in the genotype frequencies and allele frequencies of -183(G --> A).


CONCLUSIONS
The above results suggest that the D358A polymorphism of IL6R maybe correlate with type 2 diabetes in Chinese Han population.
Zhang
Ming
M
Department of Endocrinology, Second Hospital, Lanzhou University, Lanzhou, Gansu, 730030, P. R. China.
Tian
Lin-hong
LH
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D002681:China; D003924:Diabetes Mellitus, Type 2 / Q000208:ethnology / Q000235:genetics*; D005091:Exons; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D019947:Receptors, Interleukin-6 / Q000235:genetics*
D019947:Receptors, Interleukin-6