Synthesis and property of DNA labeled with fluorescent acridone.
Triphosphate of a thymidine analogue bearing acridone was prepared from a modified thymidine nucleotide with a terminal amino group at C5 position and an acridone derivative as a new fluorecsent-tagged nucleotide. The acridone-tagged nucleotide was incorporated into DNA enzymatically during PCR using KOD Dash DNA polymerase. Further, we introduced the acridone derivative into an amino-modified DNA chemically by post-synthetic modification, and investigated their fluorescence properties and hybridization ability. The new fluorescent-labeled DNA bearing acridone will be useful as a DNA probe.
Hasegawa
Tomoya
T
Department of Applied Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-chou, Kiryu, Gunma 376-8515, Japan.
Shoji
Atsusi
A
Kuwahara
Masayasu
M
Ozaki
Hiroaki
H
Sawai
Hiroaki
H
D000166:Acridines / Q000138:chemical synthesis / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D013696:Temperature; D013942:Thymine Nucleotides / Q000138:chemical synthesis / Q000737:chemistry*
C522085:5-(6-(9-oxoacridin-2-ylacetamido)hexylaminocarbonylmethyl)cytidine 5'-triphosphate; D000166:Acridines; D015342:DNA Probes; D005456:Fluorescent Dyes; D013942:Thymine Nucleotides