Properties of ferrocene-polyamide compounds as redox active DNA binding molecules toward the SNPs detection.
Ferrocene-polyamides having not only sequence discrimination ability but also a redox mediator were synthesized. The affinity and selectivity of these compounds for a DNA duplex having a cognate GCG sequence were investigated by CD spectroscopy. Furthermore, the binding of the ferrocene-polyamides to DNA duplexes immobilized to a gold electrode was detected by cyclic voltammetry (CV) experiments. The electrochemical studies revealed that the fully matched DNA duplex having a GCG sequence showed the largest electric current, and the introduction of a mismatch site decreased the current significantly. These results suggested the potential of the ferrocene-polyamide compounds as new electrochemical ligands for electrochemical SNPs detection.
Mizuta
Masahiro
M
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan.
Terada
Takeshi
T
Seio
Kohji
K
Sekine
Mitsuo
M
D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism*; D000075163:Metallocenes; D009692:Nucleic Acid Heteroduplexes; D009757:Nylons / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D005296:Ferrous Compounds; D000075163:Metallocenes; D009692:Nucleic Acid Heteroduplexes; D009757:Nylons; D004247:DNA; C004998:ferrocene