Screening of a glutamic acid-binding aptamer from arginine-modified DNA library.
A binder that is specific for a target will be applicable to detection of a variety of molecules such as bioactive substances, environmental contaminants and so on. Nucleic acid aptamers obtained by in vitro selection method are interesting and promising binders. To enhance the ability of nucleic acid aptamers, we prepared a combinatorial modified DNA library containing arginyl uracil base by using arginyl dUTP, then attempted screening of glutamic acid-binding aptamers from the library by in vitro selection method.
Kuwahara
Masayasu
M
Department of Applied Chemistry, Fuclty of Engneering, Gunma University, 1-5-1 Tenjin-chou, Kiryu, Gunma 376-8515, Japan.
Ohsawa
Kazuomi
K
Kasamatsu
Toshiyuki
T
Shoji
Atsushi
A
Sawai
Hiroaki
H
Ozaki
Hiroaki
H
D052158:Aptamers, Peptide / Q000032:analysis* / Q000737:chemistry; D001120:Arginine / Q000031:analogs & derivatives* / Q000737:chemistry; D001665:Binding Sites; D003856:Deoxyuracil Nucleotides / Q000737:chemistry*; D015723:Gene Library; D018698:Glutamic Acid / Q000032:analysis*; D052156:SELEX Aptamer Technique
D052158:Aptamers, Peptide; D003856:Deoxyuracil Nucleotides; C520188:arginyl-5-((6-aminohexylcarbamoyl)methyl)-deoxyuridine triphosphate; D018698:Glutamic Acid; D001120:Arginine