Apoptosis of canine mammary tumor cells induced by small interfering RNA (siRNA) against Bcl-xL gene.
The inhibition of Bcl-xL mRNA expression and the acceleration of apoptotic cell rates in canine mammary tumor cell line (CF33) by the small interfering RNA (siRNA) were analyzed. The level of Bcl-xL transcripts in CF33 was decreased when cultured with siRNA, suggesting that siRNA might inhibit the expression of Bcl-xL mRNA in the CF33. Apoptotic cell rates in CF33 cultured with siRNA in Oligofectamine medium, with double strand RNA in Oligofectamine medium, without siRNA in Oligofectamine medium and in DMEM alone were 60.9%, 30%, 28.7% and 11.6% at 48-hr incubation, respectively, when evaluated by TUNEL assay. From these results, it was suggested that canine Bcl-xL might be an anticancer target of canine tumors.
Tsuchiya
Fumito
F
Department of Pathobiology, Nihon University School of Veterinary Medicine, Kanagawa, Japan.
Kano
Rui
R
Sano
Junichi
J
Oguma
Keisuke
K
Hasegawa
Atsuhiko
A
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D015972:Gene Expression Regulation, Neoplastic; D020287:In Situ Nick-End Labeling; D008055:Lipids; D015674:Mammary Neoplasms, Animal / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D051020:bcl-X Protein / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D008055:Lipids; D034741:RNA, Small Interfering; D051020:bcl-X Protein; C484027:oligofectamine