title: [Studies on chemical constituents in heartwood of Taxus cuspidata].
abstract: OBJECTIVE
  To study the chemical constituents in the heartwood of Taxus cuspidata.
  
  
  METHODS
  Silica gel column chromatography, preparative HPLC and preparative TLC were used to isolate and purify the chemical constituents; 1H- and 13C-NMR spectroscopic methods were used for structural identification.
  
  
  RESULTS
  Ten compounds, taxinine (1), taxusin (2), beta-sitosterol (3), 1 beta-hydroxybaccatin I (4), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl) butanoyloxy-4 (20), 11-taxadiene (5), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl-3'-hydroxy-butanoyloxyl-4 (20), 11-taxadiene (yunnanxane) (6), 9alpha, 10beta, 13alpha-triacetoxy-5alpha-cinnamoyltaxa-4 (20), 11-diene (7), 2-deacetoxytaxinine J (8), taxezopidine G (9), 2alpha, 7beta, 9alpha, 10beta, 13alpha-pentaacetoxyl-taxa-4 (20), 11-dien-5-ol (5-decinnamoyltaxinine J) (10), were isolated and identified from the heartwood of T. cuspidata.
  
  
  CONCLUSIONS
  Three taxanes, 1 beta-hydroxybaccatin I (4), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl-3'-hydroxy-butanoyloxy)-4 (20), 11-taxadiene (yunnanxane) (6), and 2alpha, 7beta, 9alpha, 10beta, 13alpha-pentaacetoxyltaxa-4 (20) , 11-dien-5-ol (10), were obtained from this plant for the first time.
authors.affiliation: Department of Medicinal Natural Product Chemistry, School of Pharmaceutical Sciences, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Cong-Mei
authors.initials: CM
authors.lastname: Cao
authors.forename: Chang-Hong
authors.initials: CH
authors.lastname: Huo
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Zhao
authors.forename: Xiao-Tang
authors.initials: XT
authors.lastname: Si
authors.forename: Zuo-Ping
authors.initials: ZP
authors.lastname: Li
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Shi
mesh_terms: D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*; D006576:Heterocyclic Compounds, 4 or More Rings; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D043823:Taxoids / Q000737:chemistry / Q000302:isolation & purification*; D020946:Taxus / Q000737:chemistry*
chemical_list: C488272:1beta-hydroxy baccatin I; C523497:2alpha, 7beta, 9alpha, 10beta, 13alpha-pentaacetoxyltaxa-4 (20) , 11-dien-5-ol; D004224:Diterpenes; D006576:Heterocyclic Compounds, 4 or More Rings; D043823:Taxoids; C076298:yunnanxane; C107336:taxusin; C473166:taxinine