Controlling the expression ratio of two proteins by inserting a terminator between the two genes.
An approach was developed in controlling the expression ratio of two genes encoded in DNA by inserting a terminator sequence between the two genes. Escherichia coli ribosomal RNA operon T1 terminator, or E. coli tryptophane attenuater was introduced between two green fluorescent protein (GFP) mutants: ultraviolet-optimized mutant (GFPuv: F99S, M153T, V163A) and blue-shifted mutant (BFP: F64L, S65T, T145F). The ratio of the fluorescence intensity of BFP to GFPuv after transcription and translation reflected the termination efficiency of the transcriptional termination activity of the inserted terminator.
Nojima
Takahiko
T
Center for International Research on MicroMechatronics, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
Lin
Angela C
AC
Yamamoto
Takatoki
T
Endo
Isao
I
Fujii
Teruo
T
D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D049408:Luminescent Agents / Q000378:metabolism; D014176:Protein Biosynthesis; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D049408:Luminescent Agents; D049452:Green Fluorescent Proteins