A novel approach for generating full-length, high coverage allele libraries for the analysis of protein interactions.
The yeast reverse two-hybrid method was developed to identify mutations disrupting protein-protein interactions. Adoption of the method has been slow, in large part, due to the high frequency of truncation and frameshift mutants typically observed with current protocols. We have developed a new strategy, based on in vitro recombinational cloning and full-length selection in Escherichia coli, to eliminate this background and dramatically increase the efficiency of the reverse two-hybrid protocol. The method was tested by generating an allele library of MyoD1 and selecting for alleles with defective interaction with Id1. Our results confirm that most of the interaction-defective alleles contain a single point mutation in the known interaction domain, the basic helix-loop-helix region. Moreover analysis of the crystal structure of MyoD reveals that the majority of these mutations occurred at the interaction interface. The results obtained using this novel approach for allele library generation demonstrate a significant advancement in the application of yeast reverse two-hybrid screens. Furthermore this method is applicable to any loss-of-function mutant screen where truncated proteins are a source of high background.
Gray
Phillip N
PN
Invitrogen Corporation, Carlsbad, California 92008, USA. phillipngray@gmail.com
Busser
Katherine J
KJ
Chappell
Thomas G
TG
D000483:Alleles; D000818:Animals; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009154:Mutation; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D020798:Two-Hybrid System Techniques
C497455:Idb1 protein, mouse; D051798:Inhibitor of Differentiation Protein 1; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein