[The expression of enhanced green fluorescent protein gene on cultured human fetal retinal progenitor cells].
OBJECTIVE
To investigate the transfer efficiency and transient expression of plasmid vector coding enhanced green fluorescent protein (EGFP)gene pEGFP-N1 which was transferred into primary cultured human fetal retinal progenitor cells. To build up a tracking method for study of retinal progenitor cells transplantation.


METHODS
pEGFP-N1 plasmid was amplified in E.coli, primary cultured human fetal retinal progenitor cells transferred with pEGFP-N1 by means of CaCl2. The transfer efficiency was evaluated by flow cytometry. Transient expression in vitro was observed by fluorescent microscope and laser scanning confocal microscope.


RESULTS
The transfer efficiency of pEGFP-N1 into primary cultured human fetal retinal progenitor cells could maximumly reach to 32.45% during 48 hours. There was obvious expression of pEGFP-N1 in vitro at 48th hour after transferring.


CONCLUSIONS
pEGFP-N1 is an ideal transient expression vector for primary cultured human fetal retinal progenitor cells in vitro. It is a useful tracer for transplantation of retinal progenitor cells.
Kang
Qianyan
Q
Research Center for Neuroscience, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xian 710061, China.
Liu
Yong
Y
Zhang
Jin
J
Liu
Jianxin
J
Shi
Qindong
Q
Tian
Yingfang
Y
Xiao
Xinli
X
Zhang
Dianzeng
D
Qian
Yihua
Y
D002478:Cells, Cultured; D004622:Embryo, Mammalian; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D012160:Retina / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014162:Transfection
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins