Aminated poly(L-histidine) as new pH-sensitive DNA carrier.
The endosomal escape of the polyion complex between DNA and polycation is one of important factors for efficient gene expression. Here we have examined whether the poly(L-histidine) with several aminoethyl groups, i.e., aminated poly(L-histidine), possessed the pH-sensitive properties as DNA carrier. The polyion complex formation of the aminated poly(L-histidine) with DNA was affected by the endosomal pH of the medium, leading to significant transfection activity.
Asayama
Shoichiro
S
Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan.
Hamaya
Ai
A
Sekine
Takashi
T
Kawakami
Hiroyoshi
H
Nagaoka
Shohji
S
D000588:Amines / Q000737:chemistry*; D045744:Cell Line, Tumor; D004247:DNA / Q000378:metabolism*; D004337:Drug Carriers; D006461:Hemolysis; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration
D000588:Amines; D004337:Drug Carriers; C033223:polyhistidine; D006639:Histidine; D004247:DNA