title: Photoregulation of in vitro transcription/translation of GFP by tethering an azobenzene to T7 promoter.
abstract: Based on a previous study of photo-regulation of transcription by T7 RNA polymerase, the regulatory effect of azobenzene on in vitro green fluorescent protein (GFP) production was studied by introduction of a D-threoninol-tethered p-azobenzene into the non template strand of the T7 promoter in a GFP template. By measurement of the resulting GFP fluorescence intensity after the in vitro transcription/translation coupled reaction, it is found that photo-regulation of GFP gene expression can be achieved in a position-dependent manner by trans-cis isomerization of azobenzene upon UV irradiation.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Jingmin
authors.initials: J
authors.lastname: Zhou
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Liu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Asanuma
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D001391:Azo Compounds / Q000378:metabolism*; D017123:Bacteriophage T7 / Q000235:genetics; D001483:Base Sequence; D005786:Gene Expression Regulation / Q000528:radiation effects; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics*; D008027:Light; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014176:Protein Biosynthesis / Q000528:radiation effects*; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000528:radiation effects*
chemical_list: D001391:Azo Compounds; D049452:Green Fluorescent Proteins; C009850:azobenzene