Capillary isoelectric focusing--useful tool for detection of the biofilm formation in Staphylococcus epidermidis.
The biofilm formation is an important factor of S. epidermidis virulence. Biofilm-positive strains might be clinically more important than biofilm-negative ones. Unlike biofilm-negative staphylococci, biofilm-positive staphylococci are surrounded with an extracellular polysaccharide substance. The presence of this substance on the surface can affect physico-chemical properties of the bacterial cell, including surface charge. 73 S. epidermidis strains were examined for the presence of ica operon, for the ability to form biofilm by Christensen test tube method and for the production of slime by Congo red agar method. Isoelectric points (pI) of these strains were determined by means of Capillary Isoelectric Focusing. The biofilm negative strains focused near pI value 2.3, while the pI values of the biofilm positive strains were near 2.6. Isoelectric point is a useful criterion for the differentiation between biofilm-positive and biofilm-negative S. epidermidis strains.
Ruzicka
Filip
F
Department of Microbiology, Faculty of Medicine, Masaryk University, 601 77 Brno, Czech Republic. fruzic@fnusa.cz
Horka
Marie
M
Hola
Veronika
V
Votava
Miroslav
M
D000581:Amidohydrolases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms / Q000254:growth & development*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods*; D007526:Isoelectric Point; D009876:Operon; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D013212:Staphylococcus epidermidis / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D014465:Ultrasonics
D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial; D000581:Amidohydrolases; C497363:poly-N-acetylglucosamine deacetylase