Comparison of single and touchdown PCR protocols for detecting Pneumocystis jirovecii DNA in paraffin-embedded lung tissue samples.
De la Horra
Carmen
C
Reina Mercedes Investigation Unit, Seville, Spain.
Varela
Jose M
JM
Friaza
Vicente
V
Respaldiza
Nieves
N
Muñoz-Lobato
Fernando
F
Montes-Cano
Marco A
MA
Calderon
Enrique
E
Medrano
Francisco J
FJ
D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D006801:Humans; D008168:Lung / Q000382:microbiology*; D016612:Paraffin Embedding; D045363:Pneumocystis carinii / Q000235:genetics* / Q000302:isolation & purification; D011020:Pneumonia, Pneumocystis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004271:DNA, Fungal; D004275:DNA, Ribosomal