Sequence-specific recognition of double-stranded DNA by cooperative strand invasion.
A remarkable feature of peptide nucleic acid (PNA) is its ability to recognize some sequences within duplex DNA by strand invasion. In order to improve binding properties of PNA triplex invasion, we tested the effect of cooperativity on the sequence specificity. A PNA targeting six bases within duplex DNA stringently recognized 12 base-pair homopurine site at a single base level.
Sugiyama
Toru
T
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Imamura
Yasutada
Y
Hakamata
Wataru
W
Kurihara
Masaaki
M
Kittaka
Atsushi
A
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D020135:Peptide Nucleic Acids; C051802:triplex DNA; D004247:DNA