[The effects of calmodulin on the lipid-binding activity of CaM-binding protein-10 and maize non-specific lipid transfer protein].
Fluorescence-marked lipid binding experiment shows that CaMBP-10 has typical lipid binding feature of non-specific lipid transfer protein (nsLTP). We have compared the effects of calmodulin (CaM) on the lipid-binding activity of CaMBP-10 and maize nsLTP. Different influences were found in the presence of either Ca(2+) or EGTA. W-7 and TFP could abolish the influence of CaM. Therefore, it is suggested that CaM could interact specifically with both CaMBP-10 and maize nsLTP. Probably, there are different CaM regulatory mechanisms between CaMBP-10 and maize nsLTP.
Xie
Wan-Qin
WQ
Department of Biochemistry and Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071, China.
Zhao
Li-Qing
LQ
Bai
Wen-Yan
WY
Li
Zhen-Peng
ZP
Zhao
Yu-Long
YL
Li
Cui-Feng
CF
D000595:Amino Acid Sequence; D001667:Binding, Competitive / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology*; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D007700:Kinetics; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000187:drug effects; D017386:Sequence Homology, Amino Acid; D014268:Trifluoperazine / Q000494:pharmacology; D003313:Zea mays / Q000235:genetics / Q000378:metabolism*
D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins; C432363:calmodulin-binding protein BP-10; C072087:sterol carrier proteins; D014268:Trifluoperazine; D004533:Egtazic Acid; D002118:Calcium