title: Synthesis of oligonucleotide containing 4'-o-nitrobenzyloxythymidine and its reactivity.
abstract: We synthesized oligonucleotide (1) containing 4'-o-nitrobenzyloxythymidine (2), which was prepared by reaction of 4',5'-unsaturated thymidine with m-CPBA followed by Lewis-acid catalyzed addition of o-nitrobenzylalcohol. The duplex stability of 1 with the complementary oligonucleotide was studied by Tm measurement. Photochemical reaction of 1 to generate C4'-oxidized abasic site containing oligonucleotide (3) was studied.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Aso
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Fukuda
authors.forename: Kazuteru
authors.initials: K
authors.lastname: Usui
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suemune
mesh_terms: D002851:Chromatography, High Pressure Liquid; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D010777:Photochemistry; D013936:Thymidine / Q000031:analogs & derivatives* / Q000737:chemistry*
chemical_list: C521143:4'-o-nitrobenzyloxythymidine; D009841:Oligonucleotides; D013936:Thymidine