Low selection of preneoplastic hepatocytes after treatment with the 2-acetylaminofluorene diet-partial hepatectomy regimen in the liver of hepatocarcinogenesis-resistant DRH strain rats.
In hepatocarcinogenesis-resistant DRH rats, preneoplastic hepatocytic lesions are smaller than those of usual rats during carcinogenesis. When preneoplastic hepatocytes from DRH and Donryu (original strain of DRH) were reciprocally transplanted into the livers of DRH and Donryu treated with 2-acetylaminofluorene (2-AAF) diet/two-thirds hepatectomy (PH), the Donryu cells formed small colonies within the DRH liver, whereas the DRH cells formed large colonies within the Donryu liver. The DRH liver showed less degree of oval cell proliferation after treatment with 2-AAF and PH, and DRH hepatocytes were more resistant to the growth-inhibitory effect of 2-AAF after PH. Furthermore, DRH hepatocytes were generally resistant to cytotoxicity of hepatotoxins. The tissue environment of the DRH liver, therefore, is less effective for selective growth of preneoplastic hepatocytes during the carcinogen treatment, which is probably a major cause of the hepatocarcinogenesis-resistance in DRH rats.
Imai
Koji
K
Department of Pathology, Asahikawa Medical College, 2-1-1-1 East, Midorigaoka, Asahikawa 078-8510, Japan.
Yamamoto
Masahiro
M
Tanaka
Hiroki
H
Hashimoto
Norikazu
N
Miyakoshi
Masaaki
M
Honmou
Satoshi
S
Yoshie
Masumi
M
Tamakawa
Susumu
S
Yaginuma
Yuji
Y
Kasai
Shinichi
S
Ogawa
Katsuhiro
K
D015073:2-Acetylaminofluorene / Q000008:administration & dosage; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage; D048708:Cell Growth Processes / Q000187:drug effects; D020022:Genetic Predisposition to Disease; D005982:Glutathione Transferase / Q000096:biosynthesis; D006498:Hepatectomy; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology / Q000473:pathology* / Q000637:transplantation; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D008297:Male; D009368:Neoplasm Transplantation; D011230:Precancerous Conditions / Q000139:chemically induced / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains
D002273:Carcinogens; D015073:2-Acetylaminofluorene; D005982:Glutathione Transferase