title: Identification of alterations in DNA copy number in host stromal cells during tumor progression.
abstract: The interactions between cancer cells and the surrounding host stromal tissue play a critical role in tumor progression and metastasis, but the molecular nature of this relationship remains largely uncharacterized. Furthermore, although genetic changes of neoplastic cells in tumors contribute significantly to tumor progression, it is not known whether similar changes occur in the adjacent host stromal microenvironment and whether they contribute to or inhibit tumorigenesis. To address this question in an unbiased and genome-wide manner, we applied high-resolution DNA copy number analysis to murine stromal DNA isolated from human xenograft tumors that were formed in immunodeficient mice. We show that numerous amplifications and deletions are found within the host stromal microenvironment, suggesting that alterations in host DNA copy number can occur and may play a significant role in modifying tumor-stromal interactions.
authors.affiliation: Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA. rpelham@princeton.edu
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Pelham
authors.forename: Linda
authors.initials: L
authors.lastname: Rodgers
authors.forename: Ira
authors.initials: I
authors.lastname: Hall
authors.forename: Robert
authors.initials: R
authors.lastname: Lucito
authors.forename: Ken C Q
authors.initials: KC
authors.lastname: Nguyen
authors.forename: Nicholas
authors.initials: N
authors.lastname: Navin
authors.forename: James
authors.initials: J
authors.lastname: Hicks
authors.forename: David
authors.initials: D
authors.lastname: Mu
authors.forename: Scott
authors.initials: S
authors.lastname: Powers
authors.forename: Michael
authors.initials: M
authors.lastname: Wigler
authors.forename: David
authors.initials: D
authors.lastname: Botstein
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004247:DNA / Q000235:genetics*; D018450:Disease Progression; D018628:Gene Dosage / Q000235:genetics*; D016678:Genome / Q000235:genetics; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology*; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D004247:DNA