Role of phosphoenolpyruvate in the NADP-isocitrate dehydrogenase and isocitrate lyase reaction in Escherichia coli.
Phosphoenolpyruvate inhibited Escherichia coli NADP-isocitrate dehydrogenase allosterically (Ki of 0.31 mM) and isocitrate lyase uncompetitively (Ki' of 0.893 mM). Phosphoenolpyruvate enhances the uncompetitive inhibition of isocitrate lyase by increasing isocitrate, which protects isocitrate dehydrogenase from the inhibition, and contributes to the control through the tricarboxylic acid cycle and glyoxylate shunt.
Ogawa
Tadashi
T
Department of Biochemistry, Aichi Medical University School of Medicine, Aichi 489-1195, Japan.
Murakami
Keiko
K
Mori
Hirotada
H
Ishii
Nobuyoshi
N
Tomita
Masaru
M
Yoshin
Masataka
M
D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D007521:Isocitrate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007522:Isocitrate Lyase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007523:Isocitrates / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D009249:NADP / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000378:metabolism* / Q000494:pharmacology
D007523:Isocitrates; D009249:NADP; D010728:Phosphoenolpyruvate; C034219:isocitric acid; D007521:Isocitrate Dehydrogenase; D007522:Isocitrate Lyase