title: Developmental expression of transferrin binding protein in oligodendrocyte lineage cells of the embryonic chick spinal cord.
abstract: Oligodendrocytes develop from precursor cells in the neuroepithelium of the ventral ventricular zone. Oligodendrocytes in the different stages of development are characterized by expression of a number of different marker molecules such as myelin genes, growth factors, and specific antigens. We have previously identified that transferrin binding protein (TfBP), a member of heat shock protein 90 families, is a novel avian ER-associated membrane protein that is specifically localized in oligodendrocytes in adult chicken CNS. In this study we describe the developmental expression of TfBP in the embryonic chick spinal cord. A few, distinct, TfBP+ cells appeared at the lateral margin of the subventricular neuroepithelium of the spinal cord at E7. Thereafter, some TfBP+ cells, exhibited a migrative form of unipolar or bipolar shape occurred around E8 in the mantle layer, midway between the neuroepithelium and the marginal layer of the primitive spinal cord. Thereafter, the TfBP+ cells rapidly increased in number as well as their staining intensity, and overall distribution of TfBP+ cells at E15 was comparable to that of a mature spinal cord. Our observations suggest that TfBP is expressed in the subpopulation of oligodendrocyte lineage in the development and a putative role of TfBP in relation to transferrin and iron trafficking is considered.
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine, 28, Yongon-Dong, Chongno-Gu, Seoul 110-799, Korea.
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Park
authors.forename: Ha Seong
authors.initials: HS
authors.lastname: Lim
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Roh
authors.forename: Dong Woon
authors.initials: DW
authors.lastname: Kim
authors.forename: Gye Sun
authors.initials: GS
authors.lastname: Jeon
authors.forename: Sa Sun
authors.initials: SS
authors.lastname: Cho
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019070:Cell Lineage; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009836:Oligodendroglia / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D013116:Spinal Cord / Q000196:embryology; D033863:Transferrin-Binding Proteins / Q000096:biosynthesis*
chemical_list: D033863:Transferrin-Binding Proteins