[Effect of several physiochemical factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension culture].
OBJECTIVE
To illustrate the effects of several physiochemical factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension cultures.


METHODS
High performance liquid chromatography and plant tissue culture were applied.


CONCLUSIONS
Cell growth and isoflavone accumulation were significantly stimulated in P. lobata cell suspension cultures by the increase of the sucrose concentration. Maintaining the pH value at the range over 5. 4 to 5. 8 was most suitable for isoflavone accumulation in P. lobata cell suspension cultures. Cell dried weight and isoflavone accumulation decreased sharply with the increase of the treated concentration of active carbon, while XAD-4 significantly stimulated cell growth and isoflavone accumulation.
Fang
Cong-Bing
CB
Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Agriculture, Anhui Agricultural University, Hefei 230036, China.
Li
He-Qin
HQ
Wan
Xiao-Chun
XC
Jiang
Chang-Jun
CJ
D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002606:Charcoal / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000378:metabolism*; D010946:Plants, Medicinal / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D011137:Polystyrenes; D011145:Polyvinyls; D029908:Pueraria / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D013395:Sucrose / Q000494:pharmacology*
D003470:Culture Media; D007529:Isoflavones; D011137:Polystyrenes; D011145:Polyvinyls; D002606:Charcoal; C012101:XAD-4 resin; D013395:Sucrose