Susceptibility testing of Mycobacterium tuberculosis by broth microdilution method: a rapid alternative method.
A total of 50 clinical isolates of Mycobacterium tuberculosis were tested by the proportion method and the broth microdilution method (BMM) for primary antitubercular drugs. The results were obtained after 14 days of incubation. There was a 100% agreement for all drugs except streptomycin, which showed a 96% agreement. Thus, the BMM was found to be a rapid, reliable, and less labor-intensive method to determine MIC.
Sethi
Sunil
S
Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India.
Mandal
Jharna
J
Kumar
Parveen
P
Kumar
Shiv
S
Meharwal
Sandeep
S
Jindal
Surinder K
SK
Sharma
Meera
M
D000995:Antitubercular Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
D000995:Antitubercular Agents