Stimulation of transit-peptide release and ATP hydrolysis by a cochaperone during protein import into chloroplasts.
Three components of the chloroplast protein translocon, Tic110, Hsp93 (ClpC), and Tic40, have been shown to be important for protein translocation across the inner envelope membrane into the stroma. We show the molecular interactions among these three components that facilitate processing and translocation of precursor proteins. Transit-peptide binding by Tic110 recruits Tic40 binding to Tic110, which in turn causes the release of transit peptides from Tic110, freeing the transit peptides for processing. The Tic40 C-terminal domain, which is homologous to the C terminus of cochaperones Sti1p/Hop and Hip but with no known function, stimulates adenosine triphosphate hydrolysis by Hsp93. Hsp93 dissociates from Tic40 in the presence of adenosine diphosphate, suggesting that Tic40 functions as an adenosine triphosphatase activation protein for Hsp93. Our data suggest that chloroplasts have evolved the Tic40 cochaperone to increase the efficiency of precursor processing and translocation.
Chou
Ming-Lun
ML
Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan.
Chu
Chiung-Chih
CC
Chen
Lih-Jen
LJ
Akita
Mitsuru
M
Li
Hsou-min
HM
D000255:Adenosine Triphosphate / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D021381:Protein Transport
D029681:Arabidopsis Proteins; D006360:Heat-Shock Proteins; C105426:Hip chaperone; D008565:Membrane Proteins; D018832:Molecular Chaperones; D010446:Peptide Fragments; C499578:Tic110 protein, Arabidopsis; C476397:Tic40 protein, Arabidopsis; D000255:Adenosine Triphosphate