Use of steroid profiles in determining the cause of adrenal insufficiency.
OBJECTIVE
A cortisol response to adrenocorticotropin injection is the standard test for diagnosing adrenal insufficiency. Multiple steroid hormones can now be accurately measured by tandem mass spectrometry in a single sample. The study objective was to determine whether a steroid profile, created by simultaneous measurement of 10 steroid hormones by tandem mass spectrometry, would help determine the cause of adrenal insufficiency.


METHODS
A 10-steroid profile was measured by tandem mass spectrometry during the performance of a standard high dose cortrosyn stimulation test. The steroids were measured at baseline, 30, and 60min following synthetic adrenocorticotropin injection. Adrenal insufficiency was defined as a peak cortisol level of less than 20microg/dL. Testing was conducted in the general clinical research center of a university medical center. Normal volunteers, patients suspected of having adrenal insufficiency, and patients with known adrenal insufficiency participated.


RESULTS
Our results showed that adrenal insufficiency of any cause was adequately diagnosed using the response of 11-deoxycortisol, dehydroepiandrosterone, or these analytes combined in a two-steroid profile. A three-steroid profile yielded a test with 100% accuracy for discriminating primary adrenal insufficiency from normal status. Primary adrenal insufficiency was well separated from secondary adrenal insufficiency using only a single aldosterone value. 11-Deoxycortisol, dehydroepiandrosterone, and a two-steroid profile each provided fair discrimination between secondary adrenal insufficiency and normal status.


CONCLUSIONS
We conclude that stimulated levels of aldosterone, 11-deoxycortisol, dehydroepiandrosterone, and a two- or three-steroid profile provided additional discrimination between states of adrenal sufficiency and insufficiency. It is proposed that a steroid profile measuring cortisol, aldosterone, 11-deoxycortisol, and dehydroepiandrosterone would potentially improve the ability to determine the cause of adrenal insufficiency.
Holst
Jennifer P
JP
Center for Diabetes and Endocrinology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA. jj@bc.georgetown.edu
Soldin
Steven J
SJ
Tractenberg
Rochelle E
RE
Guo
Tiedong
T
Kundra
Priya
P
Verbalis
Joseph G
JG
Jonklaas
Jacqueline
J
D000305:Adrenal Cortex Hormones / Q000032:analysis / Q000097:blood*; D000309:Adrenal Insufficiency / Q000097:blood / Q000175:diagnosis* / Q000209:etiology*; D000324:Adrenocorticotropic Hormone; D000328:Adult; D000450:Aldosterone / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D003350:Cortodoxone / Q000097:blood / Q000493:pharmacokinetics; D003687:Dehydroepiandrosterone / Q000097:blood / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood / Q000493:pharmacokinetics; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013268:Stimulation, Chemical; D053719:Tandem Mass Spectrometry
D000305:Adrenal Cortex Hormones; D003687:Dehydroepiandrosterone; D000450:Aldosterone; D000324:Adrenocorticotropic Hormone; D003350:Cortodoxone; D006854:Hydrocortisone