bft gene subtyping in enterotoxigenic Bacteroides fragilis isolated from children with acute diarrhea.
Enterotoxigenic Bacteroides fragilis (ETBF) strains are associated with diarrhea disease in farm animals and young children. In this study, the bft gene subtyping from ETBF strains recovered from one immunodeficient and two immunocompetent children with diarrhea were determined. Thirteen ETBF strains were isolated and by using a multiplex-PCR their bft subtypes were determined. All 13 ETBF strains harbored the bft-1 subtype and by AP-PCR they were clustered in the same group I. This study shows that ETBF strains can be present in acute diarrhea and that bft-1 subtype is often present in these organisms. However, further studies are needed to evaluate the role of this bft-1 subtype in the pathogenesis of diarrhea.
Nakano
Viviane
V
Laboratório de Anaeróbios, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil.
Gomes
Tânia A T
TA
Vieira
Mônica A M
MA
Ferreira
Rita de Cássia
Rde C
Avila-Campos
Mario Julio
MJ
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000208:Acute Disease; D001427:Bacterial Toxins / Q000235:genetics*; D001441:Bacteroides fragilis / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D002648:Child; D003967:Diarrhea / Q000382:microbiology*; D006678:HIV; D019073:HT29 Cells; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction
D001427:Bacterial Toxins; C405031:Bacteroides fragilis toxin; D008666:Metalloendopeptidases