[An experimental study on the inhibition effect of corneal allograft rejection in rat by local application of Tetrandrine eye drops].
OBJECTIVE
To investigate the effect and the best concentration of Tetrandrine (Tet) eye drops in inhibition of the corneal allograft rejection in rat.


METHODS
Eighty SD rats were randomly divided for being treated with 0.3%, 0.5%, 1.0% Tetrandrine eye drops, respectively. 0.1% Dexamethasone eye drops-treated and control groups. After the rat model of heterograft-heterotopic graft rejection was made, slit-limp microscope and HE staining, light microscope and microphoto-analyses technique were used to observe the neovascular formation and inflammation at different time points at post-operation.


RESULTS
The types of the cells in the surgical area were mainly lymphocytes and mononuclear-macrophage. Corneal neovascularization and inflammatory cells were significantly inhibited in 0.5% Tetrandrine eye drops-treated group (P < 0.05), compared with control group at 7 d, 14 d, 21 d, 28 d post-operation.


CONCLUSIONS
The results indicate that 0.5% Tet eye drops significantly inhibit the corneal allograft rejection in rats without obvious side effects.
Zhang
Yue
Y
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Zhang
Shi
S
Chen
Xiaoping
X
Hu
Shixing
S
D000470:Alkaloids / Q000008:administration & dosage* / Q000627:therapeutic use; D000818:Animals; D044182:Benzylisoquinolines / Q000008:administration & dosage* / Q000627:therapeutic use; D003315:Cornea / Q000473:pathology; D016039:Corneal Transplantation; D005260:Female; D006084:Graft Rejection / Q000517:prevention & control*; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D009883:Ophthalmic Solutions; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
D000470:Alkaloids; D044182:Benzylisoquinolines; D007166:Immunosuppressive Agents; D009883:Ophthalmic Solutions; C009438:tetrandrine