Preparation of porous biodegradable poly(lactide-co-glycolide)/ hyaluronic acid blend scaffolds: characterization, in vitro cells culture and degradation behaviors.
A series of poly(lactide-co-glycolide) (PLGA)/ hyaluronic acid (HA) blend with different HA composition were used to fabricate scaffolds successfully. The pores of the three dimensional scaffold were prepared by particle leaching and freeze drying. The pore size was about 50-200 microm and the porosity was about 85%. The characterizations of the scaffold, such as mechanical properties, hydrophilicity and surface morphologies were determined. Mouse 3T3 fibroblast was directly seeded on the scaffolds. The cell adhesion efficiency, cell morphology observed by scanning electron microscopy (SEM) and the degradation behavior of the blend scaffold were evaluated. In summary, the results show that the adhesion efficiency of cells on the PLGA/HA blend scaffold is higher than that on the PLGA scaffold. Moreover, the incorporation of HA in PLGA not only helps to increase the cell affinity but also tends to lead the water and nutrient into the scaffold easily.
Lee
Chih-Ta
CT
Department of Chemical Engineering National Tsing Hua, University 101, Section 2 Kuang Fu Road, Hsin Chu, Taiwan 300, ROC.
Lee
Yu-Der
YD
D016475:3T3 Cells; D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D019344:Lactic Acid / Q000378:metabolism; D051379:Mice; D011100:Polyglycolic Acid / Q000378:metabolism; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000378:metabolism; D016062:Porosity; D013499:Surface Properties
D001672:Biocompatible Materials; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D006820:Hyaluronic Acid