Chiral separation of beta-blockers after derivatization with a new chiral derivatization agent, GATC.
A new chiral derivatization agent with sugar moiety, 2,3,4,6-tetra-O-acetyl-beta-D-galactopyranosyl isothiocyanate (GATC) was synthesized. Several beta-blockers were investigated for the possible separation of the enantiomers by reversed-phase HPLC after derivatization with this new chiral derivatization agent (GATC). GATC was reacted readily with beta-blockers at room temperature and the reaction mixture could directly be injected into the HPLC system. The corresponding diastereomers were well resolved on an ODS column with acetonitrile-ammonium acetate buffer as a mobile phase and monitored at UV 254 nm. The optimization of the derivatization procedure (concentration of GATC, reaction temperature and time) and HPLC conditions (pH and ionic strength of mobile phase) were investigated and compared with GITC.
Ko
Mi Young
MY
College of Pharmacy, Kangwon National University, Chunchon 200-701, Korea.
Shin
Dae Hong
DH
Oh
Joung Weon
JW
Asegahegn
Workaferhaw Shibru
WS
Kim
Kyeong Ho
KH
D000085:Acetates; D000097:Acetonitriles; D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000302:isolation & purification*; D000588:Amines / Q000737:chemistry; D000605:Amino Alcohols / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D017879:Isothiocyanates / Q000737:chemistry*; D009005:Monosaccharides / Q000737:chemistry*; D013237:Stereoisomerism
C515643:2,3,4,6-tetra-O-acetyl-beta-D-galactopyranosyl isothiocyanate; D000085:Acetates; D000097:Acetonitriles; D000319:Adrenergic beta-Antagonists; D000588:Amines; D000605:Amino Alcohols; D007202:Indicators and Reagents; D017879:Isothiocyanates; D009005:Monosaccharides; C018824:ammonium acetate