title: A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH.
abstract: Phospholipase A(2) is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.
authors.affiliation: Department of Pathology, Oklahoma State University Center for Health Sciences, 1111 W. 17th. St. Tulsa, OK 74107-1898, USA. joseph.price@okstate.edu
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Price
mesh_terms: D000818:Animals; D001979:Bromthymol Blue; D003124:Colorimetry / Q000379:methods*; D003435:Crotalid Venoms / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D010713:Phosphatidylcholines / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2
chemical_list: D003435:Crotalid Venoms; D007202:Indicators and Reagents; D010713:Phosphatidylcholines; D010741:Phospholipases A; D054467:Phospholipases A2; D001979:Bromthymol Blue