High throughput quantification of protein expression of cancer antigens in tissue microarray using quantum dot nanocrystals.
We developed and validated a novel method for quantifying protein expression of cancer tumors in an accurate, sensitive, and high throughput format. This technique integrates quantum dots, tissue microarray, optical spectroscopy, and algorithm design for analysis of tumor biopsies. The integration of this method for tissue analysis in the clinic bears potential impact for improving the diagnosis and treatment of cancer.
Ghazani
Arezou A
AA
Division of Applied Molecular Oncology, Ontario Cancer Institute, Toronto, Ontario M5G 2M9, Canada.
Lee
Jeongjin A
JA
Klostranec
Jesse
J
Xiang
Qing
Q
Dacosta
Ralph S
RS
Wilson
Brian C
BC
Tsao
Ming S
MS
Chan
Warren C W
WC
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D007633:Keratins / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D046228:Microarray Analysis; D009368:Neoplasm Transplantation; D045663:Quantum Dots; D014183:Transplantation, Heterologous
D000951:Antigens, Neoplasm; D015820:Cadherins; D007633:Keratins; D066246:ErbB Receptors