Development of a novel DNA sensing system using DNA aptamer inhibited enzymatic activity 1.
A novel DNA detection system has been developed using the DNA aptamer inhibiting thrombin activity. The complementary strand to the target DNA was inserted into the loop region of the G-quartet structure of this adapter aiming at the disruption of it by the hybridization of the target DNA with its complementary strand in the aptamer. Using this modified aptamer, we were able to detect the target DNA by measuring thrombin activity.
Ikebukuro
Kazunori
K
Department of Biotechnology & Life Science, Tokyo University of Agriculture & Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Yoshida
Wataru
W
Sode
Koji
K
D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D001777:Blood Coagulation / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects; D013917:Thrombin / Q000037:antagonists & inhibitors*
D052157:Aptamers, Nucleotide; C081676:thrombin aptamer; D004247:DNA; D013917:Thrombin