title: Synthesis and properties of 2'-O-cyanoethylated RNA derivatives.
abstract: We developed a new protocol for the cyanoethylation of the hydroxyl function of nucleosides. A new system of Cs2CO3-tBuOH proved to be the best for the cyanoethylation. An oligonucleotide incorporating 2'-O-cyanoethylated uridine has high affinity (deltaTm = 0.6 degrees C/modification) for the complementary DNA or RNA strand than the corresponding 2'-O-methyl-RNA.
authors.affiliation: Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Saneyoshi
authors.forename: Keigo
authors.initials: K
authors.lastname: Tamaki
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
mesh_terms: D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D012313:RNA