Involvement of Drosophila Sir2-like genes in the regulation of life span.
In Drosophila melanogaster, the Sir2 gene and four Sir2-like genes have been found to be homologous to yeast SIR2 genes. To examine whether the fly Sir2, CG5216, and two Sir2-like genes, CG5085 and CG6284, affect life span, we suppressed their expression using RNAi. Decreased expression of the Sir2 and Sir2-like genes in all cells caused lethality during development. Suppression of the Sir2 in neurons and ubiquitous silencing of the Sir2-like genes shortened life spans. The effects were severer at 28 degrees C than at 25 degrees C. These results suggest that Sir2-like genes as well as Sir2 are involved in the regulation of life span in Drosophila.
Kusama
Sakiku
S
Department of Advanced Biosciences, Ochanomizu University, Tokyo, Japan.
Ueda
Ryu
R
Suda
Takeshi
T
Nishihara
Shoko
S
Matsuura
Etsuko T
ET
D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology; D006655:Histone Deacetylases / Q000235:genetics*; D008136:Longevity / Q000235:genetics*; D037761:Sirtuins / Q000235:genetics*
D029721:Drosophila Proteins; C447899:Sirt2 protein, Drosophila; D037761:Sirtuins; D006655:Histone Deacetylases