Spectroscopic analysis of AgI-DNA complex.
It has recently been reported that C-C mismatches were able to recognize Ag(I) specifically, and form Ag(I)-mediated cytosine-cytosine base pairs (C-Ag(I)-C pair). In this study, we explored DNA sequences, which are appropriate for structural studies of this new type of metal-mediated base pairs. For this experiments, we synthesized four kinds of DNA duplexes including a single C-C mismatch. By using UV spectra, we performed titration experiments of DNA duplexes with AgNO(3). Within the sequences we tested, it was found that the DNA duplex: 5'-CGCGCGTCC-3' x 5'-GGACCCGCG-3' showed 1:1 complexation of this DNA duplex and Ag(I). In addition, a DNA duplex: 5'-TAAATCTAATA-3' x 5'-TATTACATTTA-3' also exhibited a similar character. Therefore, these two DNA duplexes are interesting candidates for structural analyses.
Oda
Shuji
S
Graduate School of Pharmaceutical Sciences Tohoku University, Aobayama, Aoba-ku, Sendai 980-8578, Japan.
Komuro
Tomoyuki
T
Kondo
Yoshinori
Y
Ono
Akira
A
Tanaka
Yoshiyuki
Y
D020137:Base Pair Mismatch; D003596:Cytosine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D012834:Silver; D003596:Cytosine; D004247:DNA