title: A novel dodecapeptide from a combinatorial synthetic library exhibits potent antifungal activity and synergy with standard antimycotic agents.
abstract: There has been a marked expansion in the discovery of new antifungal peptides. This paper describes a novel dodecapeptide, H-Arg-Trp-Trp-Arg-D-Trp-D-Phe-Ile-D-Phe-His-Trp-Arg-Trp-NH(2), derived from a previously described nonapeptide and synthesized by the combinatorial approach. Further, interaction of this peptide with antifungals such as amphotericin B, flucytosine and fluconazole was studied by checkerboard analysis and time-kill assay to obtain the dynamic picture with respect to time. The best synergistic activity was observed with a combination of peptide and fluconazole, followed by peptide and flucytosine.
authors.affiliation: Division of Medicinal and Process Chemistry, Central Drug Research Institute, Lucknow-226 001, India.
authors.forename: S
authors.initials: S
authors.lastname: Duggineni
authors.forename: G
authors.initials: G
authors.lastname: Srivastava
authors.forename: B
authors.initials: B
authors.lastname: Kundu
authors.forename: M
authors.initials: M
authors.lastname: Kumar
authors.forename: A K
authors.initials: AK
authors.lastname: Chaturvedi
authors.forename: P K
authors.initials: PK
authors.lastname: Shukla
mesh_terms: D000595:Amino Acid Sequence; D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D020650:Combinatorial Chemistry Techniques; D003455:Cryptococcus neoformans / Q000187:drug effects; D004357:Drug Synergism; D004912:Erythrocytes / Q000187:drug effects; D015725:Fluconazole / Q000494:pharmacology; D005437:Flucytosine / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D000935:Antifungal Agents; D009842:Oligopeptides; D000666:Amphotericin B; D015725:Fluconazole; D005437:Flucytosine