Cloning and characterization of Rab Escort Protein (REP) from Arabidopsis thaliana.
Intracellular vesicular trafficking is regulated by Rab proteins, small GTPases that require posttranslational geranylgeranylation for biological activity. This covalent modification is catalyzed by Rab geranylgeranyl transferase (RabGGTase) and proceeds only in the presence of accessory Rab Escort Protein (REP). In this communication, we report the cloning and characterization of REP gene of Arabidopsis thaliana. Highest expression of REP mRNA was detected in leaves and flowers in contrast to stems and roots. AtREP is recognized by anti-rat REP1 serum. Interaction of AtREP with the protein substrate is presented, as well as a structural model obtained through homology modeling, based on the known structure of rat REP1.
Wojtas
M
M
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland.
Swiezewski
S
S
Sarnowski
T J
TJ
Plochocka
D
D
Chelstowska
A
A
Tolmachova
T
T
Swiezewska
E
E
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000737:chemistry*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D035264:Flowers / Q000737:chemistry; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D018515:Plant Leaves / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae; D017386:Sequence Homology, Amino Acid
D048868:Adaptor Proteins, Signal Transducing; D029681:Arabidopsis Proteins; C513166:Chm protein, rat; C513165:Rab escort protein, Arabidopsis; D011993:Recombinant Fusion Proteins