[Primary culture of human pterygia cells in vitro].
OBJECTIVE
To probe a selective method of isolate and culture of human pterygia cells in vitro.


METHODS
Fifty primary pterygia were got by operation and cut to clips less than 1 x 1 mm2, segments were digested by collagenase II for 20 minutes and by trypsin for 10 minutes under 37 degrees C, and then centrifugated for 15 minutes by 1500 rpm. The collected cells were cultured in conditioned medium for endothelial cells. After purified by scrape and density gradient centrifugation, the cells were detected and identified by immunohistochemistry and electron microscope.


RESULTS
The purity of cultured fibroblasts and endothelial cell type cells were more than 90%. CD31, CD34, and Weibel-Palade bodies were performed in these cells cytoplasm.


CONCLUSIONS
We obtained the method of culturing human pterygia fibroblasts and endothelial cell type cells and it is a feasible and convenient approach.
Hao
Shangchen
S
Key Laboratory of Ophthalmology of the Ministry of Education and Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Liu
Zuguo
Z
D000328:Adult; D000368:Aged; D018929:Cell Culture Techniques / Q000379:methods; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011625:Pterygium / Q000473:pathology*