FlAsH-based live-cell fluorescent imaging of synthetic peptides expressed in Arabidopsis and tobacco.
Genes encoding synthetic hydroxyproline-rich peptides with repetitive (Ser-Pro) units linked to an extensin signal sequence and a tetracysteine (TC) sequence were expressed transiently in tobacco, and transiently and stably in Arabidopsis under control of a strong constitutive promoter Expression of these peptides could be visualized in live cells by confocal microscopy following labeling with FlAsH- or ReAsH-EDT2 reagents.
Estévez
José M
JM
Carnegie Institution, Stanford, CA 94305, USA.
Somerville
Chris
C
D017360:Arabidopsis / Q000235:genetics*; D003545:Cysteine; D005452:Fluoresceins; D005456:Fluorescent Dyes; D018613:Microscopy, Confocal; D009942:Organometallic Compounds; D011994:Recombinant Proteins / Q000032:analysis* / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D014026:Nicotiana / Q000235:genetics*
C113276:4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D011994:Recombinant Proteins; D003545:Cysteine