Development of a continuous cell line, PBLE, from an American eel peripheral blood leukocyte preparation.
A continuous cell line, PBLE, was developed from the adherent cells in a culture of peripheral blood leukocytes from the American eel, Anguilla rostrata. The cells were grown in Leibovitz's L-15 basal medium supplemented with 20% fetal bovine serum (FBS). Under normal culture conditions at 18 degrees C, the morphology of PBLE was fibroblast-like. The cultures have been subcultured over 80 times and have been cryopreserved successfully. These cells have a diploid karyotype of 38 chromosomes, survived temperatures from 5 to 36 degrees C, and proliferated at temperatures from 5 degrees C to at least 30 degrees C. PBLE underwent apoptosis in response to gliotoxin, but did not show a respiratory burst. Results suggest that PBLE may have arisen from a circulating mesenchymal stem cell. PBLE was susceptible to Chum salmon reovirus (CSV) and supported CSV replication. Therefore this cell line should be useful in studying eel specific virus-host interactions.
Dewitte-Orr
S J
SJ
Department of Biology, University of Waterloo, Ontario, Canada.
Lepic
K
K
Bryson
S P
SP
Walsh
S K
SK
Lee
L E J
LE
Bols
N C
NC
D000811:Anguilla / Q000097:blood*; D000818:Animals; D017209:Apoptosis; D001773:Blood Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D003470:Culture Media; D007621:Karyotyping; D007962:Leukocytes / Q000166:cytology* / Q000502:physiology / Q000821:virology; D012087:Reoviridae / Q000502:physiology; D016897:Respiratory Burst
D003470:Culture Media