Systematic deletion of rRNAs for investigating ribosome architecture and function.
Ribosomal RNAs (rRNAs) form a fundamental structure of ribosome, and play crucial roles in protein biosynthesis. To investigate functional importance of RNA helices in both 16S and 23S rRNAs, we devised an E. coli genetic system that allowed us to identify and select functional deletions in rRNAs. We here describe many RNA helices in rRNAs to be shortened or completely truncated. These results suggest architectural flexibility of rRNAs in ribosome assembly and function.
Kitahara
Kei
K
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Sato
Neuza S
NS
Namba
Naoki
N
Yokota
Takuya
T
Tsujimura
Tarou
T
Suzuki
Tsutomu
T
D004926:Escherichia coli / Q000235:genetics; D020459:Genes, rRNA; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000235:genetics; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism; D017384:Sequence Deletion
D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S