Co-expression of the mosquitocidal toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis subsp. israelensis in Asticcacaulis excentricus.
The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry11Aa gene. The genes were introduced as an operon, but although mRNA was detected for both genes, no Cyt1Aa toxin was detected. Both proteins were expressed using a construct in which a promoter was inserted upstream of each gene. Recombinant A. excentricus expressing both toxins was found to be approximately twice as toxic to third instar larvae of Culex quinquefasciatus as transformants expressing just Cry11Aa.
Zheng
Dasheng
D
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
Valdez-Cruz
Norma Adriana
NA
Armengol
Gemma
G
Sevrez
Chloe
C
Munoz-Olaya
Jose Maurilio
JM
Yuan
Zhiming
Z
Orduz
Sergio
S
Crickmore
Neil
N
D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry* / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000633:toxicity*; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000633:toxicity*; D042063:Caulobacteraceae / Q000235:genetics*; D003001:Cloning, Molecular; D003465:Culex / Q000187:drug effects* / Q000254:growth & development; D004731:Endotoxins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000633:toxicity*; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000633:toxicity*; D007814:Larva / Q000187:drug effects; D009876:Operon; D010572:Pest Control, Biological; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000633:toxicity; D014169:Transformation, Bacterial
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis