Loss of fragile histidine triad protein expression in inflammatory bowel disease.
OBJECTIVE
To investigate the expression of fragile histidine triad (FHIT) protein in 64 patients with ulcerative colitis (UC) and Crohn's disease (CD), and its relation with clinicopathological data.


METHODS
Rabbit-anti-FHIT antibody was used to detect FHIT protein expression in 64 formalin-fixed, paraffin-embedded tissue specimens of inflammatory bowel disease (IBD) by citrate-microwave-streptavidin (SP)-HRP immunohistochemical method.


RESULTS
The positive FHIT protein expression was 22.79% +/- 16.16%, 42.14% +/- 16.82% in active and remittent phases of UC, 36.07% +/- 19.23% in CD, and 57.05% +/- 8.86% in normal colon mucosa. Statistically significant differences in FHIT protein expression were observed between the active and remittent phases of UC, between the active phase of UC and normal colon mucosa, as well as between the remittent phase of UC and normal colon mucosa, and between CD and normal colon mucosa.


CONCLUSIONS
Our results show that FHIT protein expression is completely absent or reduced in IBD, suggesting that the FHIT gene might be associated with the oncogenesis and progression of IBD, an early event from inflammatory conditions to carcinoma in IBD.
Xu
Chun-Mei
CM
Department of Gastroenterology, Xiangfan No.1 Hospital, 75 Jiefang Road, Xiangfan 441000, Hubei Province, China. chunmei200311@tom.com
Qiao
Chuan-Hu
CH
D017766:Acid Anhydride Hydrolases / Q000172:deficiency* / Q000235:genetics*; D000328:Adult; D000368:Aged; D001706:Biopsy; D002681:China; D003093:Colitis, Ulcerative / Q000235:genetics / Q000473:pathology; D003113:Colonoscopy; D003424:Crohn Disease / Q000235:genetics / Q000473:pathology; D005260:Female; D006801:Humans; D007249:Inflammation; D015212:Inflammatory Bowel Diseases / Q000235:genetics* / Q000473:pathology; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000172:deficiency* / Q000235:genetics*; D012189:Retrospective Studies; D012812:Sigmoidoscopy; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase