[Determination of residues of three stilbene drugs in animal tissues using gas chromatography-mass spectrometry].
A method has been developed to determine residual stilbenes such as diethylstilbestrol (DES), dienestrol (DIS) and hexestrol (HS) in animal tissues using solid phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS). The procedures for extraction, cleanup on an LC-Si solid phase extraction cartridge and derivatization of stilbenes were established and optimized. The analytes were detected by mass spectrometer with electron impact source in selected ion monitoring mode (EI/SIM), and quantified with an external standard calibration curve method. Linear calibration curves were obtained in the concentration ranges from 5 to 500 microg/L for HS and from 10 to 1000 microg/L for DES and DIS (the correlation coefficients were above 0.99). Recoveries of the stilbenes were 73.0%-86.5%, and the relative standard deviations were between 1.0% and 7.2%. The limits of detection were 0.30 microg/kg for cis-DES, 0.10 microg/kg for trans-DES and HS and 0.15 microg/kg for DIS in pork and swine liver.
Wu
Yinliang
Y
Quality Control and Inspection Center for Domestic Animal Products of Ministry of Agriculture, Beijing 100026, China. wupaddyfield@tom.com
Liu
Suying
S
Hou
Dongiun
D
Shen
Jianzhong
J
Wang
Hai
H
Shan
Jihao
J
D000818:Animals; D004028:Dienestrol / Q000032:analysis*; D004054:Diethylstilbestrol / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006589:Hexestrol / Q000032:analysis*; D052616:Solid Phase Extraction
D006589:Hexestrol; D004054:Diethylstilbestrol; D004028:Dienestrol