Anti-inflammatory prednisolone derivatives inhibit collagen synthesis and pro-alpha(1) (I) collagen promoter activity in rat skin fibroblasts.
Inhibition of wound healing by anti-inflammatory steroids is associated with decreased collagen synthesis. Methyl-20-dihydroprednisolonate has been previously reported to be anti-inflammatory without inhibiting collagen synthesis in skin when given either subcutaneously or intraperitoneally. The data we now report show that this prednisolone derivative, as well as two 9alpha fluorinated derivatives inhibit both collagen synthesis and pro-alpha(1) (I) collagen gene promoter activity in rat skin fibroblasts. Our data suggest that metabolism, absorption, or distribution of these corticosteroids results in their inability to inhibit collagen synthesis in vivo. In addition our data indicate that fluorination in the 9alpha position of the adrenal steroid nucleus is not required for the inhibition of collagen synthesis by methyl-20-dihydroprednisolonate.
Cutroneo
K R
KR
Department of Biochemistry, University of Vermont, Burlington, VT, USA.
Meisler
N
N
Counts
D F
DF