Suppression of bcr/abl chimeric gene by conjugate DNA enzymes in human cells.
Conjugate DNAzymes with polyamines and peptides were successfully prepared by solid phase fragment condensation (SPFC) and showed up to 4.2 times higher catalytic efficiency (kcat/Km). Intracellular localization of DNAzymes could be controlled by conjugated with naturally occurring signal peptides which are responsible for nuclear cytoplasmic transport of proteins. Suppression of bcr/abl chimeric gene on Philadelphia chromosome by conjugate DNA enzymes were largely enhanced in human leukemia cells.
Takamori
Kengo
K
Department of Biological and Environmental Chemistry, Kyushu School of Engineering, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
Kubo
Takanori
T
Zhelev
Zhivko
Z
Rumiana
Bakalova
B
Ohba
Hideki
H
Doi
Keiko
K
Fujii
Masayuki
M
D002384:Catalysis; D021881:DNA, Catalytic / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D016044:Fusion Proteins, bcr-abl / Q000037:antagonists & inhibitors / Q000235:genetics*; D016313:Genes, abl; D006801:Humans; D020014:K562 Cells; D007700:Kinetics; D011073:Polyamines / Q000737:chemistry; D021382:Protein Sorting Signals; D012313:RNA / Q000378:metabolism
D021881:DNA, Catalytic; D011073:Polyamines; D021382:Protein Sorting Signals; C406060:abl-bcr fusion protein, human; D012313:RNA; D016044:Fusion Proteins, bcr-abl