Redox status and expression of chemokines in the rat lungs on exposure to asbestos and asbestos substituents.
OBJECTIVE
Activity of cytoplasmatic superoxide dismutase, glutathione peroxidase,- and reductase, gamma-glutamyl transpeptidase and expression of macrophage chemoattractant protein-1 and macrophage inhibitory protein-1alpha was determined in primary culture of rat alveolar macrophages and type II pneumocytes after exposure to stone-wool, wollastonite and crocidolite (blue asbestos).


METHODS
The activity of redox enzymes was examined by RANDOX kits, chemokines were studied by ELISA.


RESULTS
The UICC crocidolite (positive control) decreased the activity of all redox enzymes and increased the expression of chemokines, whereas the two asbestos substituents did not alter the activity of redox enzymes either in the alveolar macrophages or pneumocytes. Stone-wool and wollastonite moderately increased the expression of chemokines in both cells.


CONCLUSIONS
The redox status and production of chemokines changed in the opposite direction, presumably owing to stronger toxic effect of asbestos. These data suggest that stone-wool and wollastonite, as potential substituents for asbestos, are less toxic than the human carcinogenic and fibrogenic crocidolite.
Tátrai
Erzsébet
E
National Institute of Environmental Health, Budapest, Hungary. tatrai@fjokk.hu
Brózik
Márta
M
Kováciková
Zuzana
Z
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001194:Asbestos / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D018932:Chemokine CCL2 / Q000378:metabolism; D054407:Chemokine CCL4; D018925:Chemokines / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000032:analysis / Q000378:metabolism; D005980:Glutathione Reductase / Q000032:analysis / Q000378:metabolism; D019570:Inhalation Exposure; D008168:Lung / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism*; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism
D000975:Antioxidants; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D054407:Chemokine CCL4; D018925:Chemokines; D019402:Macrophage Inflammatory Proteins; D001194:Asbestos; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase