title: Prevalance of Chlamydia trochomatis, Ureaplasma urealyticum and Mycoplasma hominis infections in the unexplained infertile women.
abstract: OBJECTIVE
  To prospectively investigate the prevalence of Chlamydia trachomatis (CT), Mycoplasma hominis (MH) and Ureaplasma urealyticum (UU) in the cervical canal and pouch of Douglas in unexplained infertile women and compare it to healthy controls in the Turkish population.
  
  
  METHODS
  A total of 31 women presenting with a history of infertility [n = 24 (77%) primary infertility, n = 7 (23%) secondary infertility] between 20 and 38 years of age and 31 women willing to have tubal ligation between 30 and 41 years of age were consecutively included into this study. Specimens were taken from intra-abdominal washings and from the cervical canal. CT, MH and UU were detected with polymerase chain reaction (PCR).
  
  
  RESULTS
  Results of 62 women were analyzed. None of the participants met the criteria for salpingitis during laparoscopy. The most common infection in the cervical canal in both groups was UU, which was detected in 13 cases of infertile patients and 11 controls (P = 0.602). Cervical chlamydial and mycoplasmic infection was detected in one case each in infertile and control patients. Neither MH nor UU were obtained from the pouch of Douglas in both groups. Only CT was present in peritoneal fluid of an infertile woman who had also a concomitant chlamydial infection in the cervical canal.
  
  
  CONCLUSIONS
  Demonstration of cervical colonization of CT by PCR may be a promising method for the detection of asymptomatic pelvic infection in patients with unexplained infertility. However, screening for MH and UU is not cost-effective due to similar low rates of detection.
authors.affiliation: Faculty of Medicine, Department of Obstetrics and Gynecology, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey. mguven@ksu.edu.tr
authors.forename: Melih A
authors.initials: MA
authors.lastname: Guven
authors.forename: Umut
authors.initials: U
authors.lastname: Dilek
authors.forename: Ozlem
authors.initials: O
authors.lastname: Pata
authors.forename: Saffet
authors.initials: S
authors.lastname: Dilek
authors.forename: Pinar
authors.initials: P
authors.lastname: Ciragil
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000150:complications / Q000453:epidemiology*; D002692:Chlamydia trachomatis; D003430:Cross-Sectional Studies; D004312:Douglas' Pouch / Q000382:microbiology; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000453:epidemiology* / Q000209:etiology / Q000382:microbiology*; D009175:Mycoplasma Infections / Q000150:complications / Q000453:epidemiology*; D019535:Mycoplasma hominis; D011446:Prospective Studies; D016869:Ureaplasma Infections / Q000150:complications / Q000453:epidemiology*; D016990:Ureaplasma urealyticum