Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier.
A novel psychrophilic, yellow-pigmented and obligate aerobic bacterium, strain 0499(T), was isolated from the China No.1 glacier. Strain 0499(T) displayed the common phenotypic and chemotaxonomic features of the genus Flavobacterium, containing menaquinone-6 (MK-6) as the major quinone and C(15 : 0), iso-C(15 : 0), C(17 : 1)omega6c and summed feature 3 (C(16 : 1)omega7c/iso-C(15 : 0) 2-OH) as the major fatty acids. Optimal growth occurred at 21 degrees C. The genomic DNA G+C content was 36.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 0499(T) was related to members of the genus Flavobacterium, sharing the highest sequence similarities with Flavobacterium succinicans (97.9 %), Flavobacterium granuli (97.4 %) and Flavobacterium hydatis (97.2 %). On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, a novel species Flavobacterium glaciei is proposed with strain 0499(T) (=CGMCC 1.5380(T)=JCM 13953(T)) as the type strain.
Zhang
De-Chao
DC
State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, P. R. China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P. R. China.
Wang
He-Xiang
HX
State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, P. R. China.
Liu
Hong-Can
HC
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P. R. China.
Dong
Xiu-Zhu
XZ
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P. R. China.
Zhou
Pei-Jin
PJ
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P. R. China.
D015373:Bacterial Typing Techniques; D002681:China; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000032:analysis; D005417:Flavobacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S