[Research of the preservation of the composite facial allograft].
OBJECTIVE
To investigate the effective method of preserving composite facial allograft so as to attenuate ischemic injury.


METHODS
The composite facial allografts were harvested from dog, perfused and preserved with 4 degrees C physiological sodium chloride and UW solution respectively. Immediately after the removal of the flap, after 12, 24, 48 h of preservation, MTT assay was used to determine the viability of several kinds of tissue, including skin, mucosa, muscle, bleed vessel, nerve and gland. The results of the two groups were compared in term of viability percentage. The pathology of several tissues were observed after 24 and 48 h of storage.


RESULTS
The viability percentage of every tissue conserved in UW solution for 48 hours was more than 75%. There was significant difference between physiological sodium chloride group and UW group (P < 0.05). Some changes, including Porous arrangement of fibers in connective tissue of skin and mucosa, hyalinization of tissue around the hair follicle and edema of cell in hair follicle, enlargement of space between muscle bundles and unclearness of boundary of acinus could be seen in physiological sodium chloride group while no significant change in UW group.


CONCLUSIONS
UW solution could be considered as preservation solution for composite facial allograft.
Liu
Qin-xiu
QX
Department of Plastic and Reconstructive Surgery, the Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China. liqfliqf@yahoo.com.cn
Li
Qing-feng
QF
Zheng
Sheng-wu
SW
Wang
Hui-yong
HY
Jiang
Hao
H
Li
Jiang
J
Zheng
Dan-ning
DN
Tang
Lu-jia
LJ
Li
Yu-ping
YP
Zhang
Di-sheng
DS
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D004285:Dogs; D005145:Face; D005260:Female; D005978:Glutathione; D007328:Insulin; D008297:Male; D019188:Organ Preservation Solutions; D011887:Raffinose; D014021:Tissue Preservation / Q000379:methods*; D014184:Transplantation, Homologous
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose