title: YCA1 participates in the acetic acid induced yeast programmed cell death also in a manner unrelated to its caspase-like activity.
abstract: Yeast cells lacking the metacaspase-encoding gene YCA1 (Deltayca1) were compared with wild-type (WT) cells with respect to the occurrence, nature and time course of acetic-acid triggered death. We show that Deltayca1 cells undergo programmed cell death (PCD) with a rate lower than that of the WT and that PCD in WT cells is caused at least in part by the caspase activity of Yca1p. Since in Deltayca1 cells this effect is lost, but z-VAD-fmk does not prevent both WT and Deltayca1 cell death, PCD in WT cells occurs via a Yca1p caspase and a non-caspase route with similar characteristics.
authors.affiliation: CNR, Istituto di Biomembrane e Bioenergetica, I-70126 Bari, Italy.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Guaragnella
authors.forename: Clara
authors.initials: C
authors.lastname: Pereira
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Sousa
authors.forename: Lucia
authors.initials: L
authors.lastname: Antonacci
authors.forename: Salvatore
authors.initials: S
authors.lastname: Passarella
authors.forename: Manuela
authors.initials: M
authors.lastname: Côrte-Real
authors.forename: Ersilia
authors.initials: E
authors.lastname: Marra
authors.forename: Sergio
authors.initials: S
authors.lastname: Giannattasio
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology*; D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000590:Amino Acid Chloromethyl Ketones; D029701:Saccharomyces cerevisiae Proteins; C476093:z-Val-Ala-Asp(Ome)-fluoromethylketone; D020169:Caspases; C456272:MCA1 protein, S cerevisiae; D019342:Acetic Acid