Novel fluorescent analogues for transmembrane movement study of polyprenyl phosphates.
In order to investigate the transmembrane movement of polyprenyl phosphate across biological membranes, NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled polyprenyl phosphate analogues were prepared. These analogues proved to be possible tools for a direct observation of the transmembrane flip-flop movement of polyprenyl phosphates by use of a sodium dithionite-quenching procedure.
Koseki
Koichi
K
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai 980-8577, Japan.
Yamashita
Satoshi
S
Takahashi
Seiji
S
Koyama
Tanetoshi
T
D055592:Biophysical Phenomena; D001703:Biophysics; D004227:Dithionite / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D005615:Freezing; D007202:Indicators and Reagents; D008081:Liposomes; D008566:Membranes / Q000378:metabolism / Q000648:ultrastructure; D008567:Membranes, Artificial; D010710:Phosphates / Q000138:chemical synthesis*; D010713:Phosphatidylcholines; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008081:Liposomes; D008567:Membranes, Artificial; D010710:Phosphates; D010713:Phosphatidylcholines; D004227:Dithionite