title: Protein pattern assembly by active control of a triblock copolymer monolayer.
abstract: We present an electrically programmable technique that can rapidly assemble proteins into high-resolution, arbitrarily shaped patterns. Controlled protein adsorption is achieved by switching the nanoscale surface arrangement of a triblock copolymer monolayer on microelectrodes in an engineered microfluidic device via an electric potential. Our technique is the first capable of configuring differential protein densities into patterns by varying the applied control voltage, providing the possibility to tune signals for complex analytes, test conditions, or quantification.
authors.affiliation: Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors.forename: Chao Yung
authors.initials: CY
authors.lastname: Fan
authors.forename: Katsuo
authors.initials: K
authors.lastname: Kurabayashi
authors.forename: Edgar
authors.initials: E
authors.lastname: Meyhöfer
mesh_terms: D000327:Adsorption; D005456:Fluorescent Dyes; D057927:Hydrophobic and Hydrophilic Interactions; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques; D020442:Poloxamer / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012235:Rhodamines; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014992:Xylenes / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D011108:Polymers; D011506:Proteins; D012235:Rhodamines; D014992:Xylenes; D020442:Poloxamer; C011055:parylene; D012710:Serum Albumin, Bovine