Electrochemical SNP detection.
Detection of mismatched base on a DNA duplex, which is important for the search of the heterozygote, achieved by the comparison with the reactivity of ferrocenylcarbodiimide (1) developed by our group. Mismatched thymine base on the 30-meric DNA duplex as a model of SNPs in lipoprotein lipase (LPL) gene, could react with 1 in 20 mM borate buffer (pH 8.5) and 0.1 M NaCl containing 20% DMSO at 37 degrees C. DNA duplex modified by 1 in the mismatched thymine bases could be detected by its shifted migration time in microTAS electrophoresis.
Watanabe
Sadayoshi
S
Department of Materials Science, Faculty of Engineering, Kyushu Institute of Technology, 1-1 Sensui-cyo, Tobata-ku, Kitakyushu-shi, Fukuoka, 804-8550, Japan.
Mukumoto
Kosuke
K
Nojima
Takahiko
T
Waki
Michinori
M
Takenaka
Shigeori
S
D020137:Base Pair Mismatch; D002234:Carbodiimides / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004586:Electrophoresis; D005296:Ferrous Compounds / Q000737:chemistry*; D008071:Lipoprotein Lipase / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
D002234:Carbodiimides; D005296:Ferrous Compounds; C517870:ferrocenyl carbodiimide; D004247:DNA; D008071:Lipoprotein Lipase