Desulfoglaeba alkanexedens gen. nov., sp. nov., an n-alkane-degrading, sulfate-reducing bacterium.
Two novel sulfate-reducing bacteria, strains ALDC(T) and Lake, which were able to oxidize n-alkanes, were isolated from a naval oily wastewater-storage facility (VA, USA) and from oilfield production water (OK, USA), respectively. The type strain (ALDC(T)) had a narrow substrate specificity and could grow only with n-alkanes (from C(6) to C(12)), pyruvate, butyrate, hexanoic acid and 4-methyloctanoic acid. Cells of strain ALDC(T) stained Gram-negative and were slightly curved, short rods with oval ends (2.5-3.0x1.0-1.4 microm), often occurring in pairs. Cells tended to form aggregates or large clusters and were non-motile and did not form endospores. Optimum growth occurred between 31 and 37 degrees C and at pH 6.5-7.2. NaCl was not required for growth, but salt concentrations up to 55 g l(-1) could be tolerated. The DNA G+C content was 53.6 mol%. Phylogenetic analysis of the 16S rRNA genes revealed that strains ALDC(T) and Lake were closely related, but not identical (99.9 % similarity). The two strains were not closely related to other known alkane-degrading, sulfate-reducing bacteria or to other genera of the Deltaproteobacteria. Therefore, it is proposed that strain ALDC(T) (=JCM 13588(T)=ATCC BAA-1302(T)) represents the type strain of a novel species and genus, with the name Desulfoglaeba alkanexedens gen. nov., sp. nov.
Davidova
Irene A
IA
Department of Botany and Microbiology, Institute for Energy and the Environment, The University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
Duncan
Kathleen E
KE
Department of Botany and Microbiology, Institute for Energy and the Environment, The University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
Choi
Ok Kyoung
OK
Department of Botany and Microbiology, Institute for Energy and the Environment, The University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
Suflita
Joseph M
JM
Department of Botany and Microbiology, Institute for Energy and the Environment, The University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
D000473:Alkanes / Q000378:metabolism*; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020564:Deltaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D009825:Oklahoma; D010084:Oxidation-Reduction; D010578:Petroleum; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D013379:Substrate Specificity; D013431:Sulfates / Q000378:metabolism*; D016999:Sulfur-Reducing Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods
D000473:Alkanes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013431:Sulfates