Influence of bradykinin B1 and B2 receptors in the immune response triggered by renal ischemia-reperfusion injury.
Bradykinin B1 receptors are exclusively expressed in inflamed tissues. For this reason, they have been related with the outcomes of several pathologies. Ischemia-reperfusion injury is caused by the activation of inflammatory and cytoprotective genes, such as macrophage chemoattractant protein-1 and heme oxygenase-1, respectively. This study was aimed to analyze the involvement of bradykinin B1 and B2 receptors (B1R and B2R) in tissue response after renal ischemia-reperfusion injury. For that, B1R (B1-/-), B2R (B2-/-) knockout animals and its control (wild-type mice, B1B2+/+) were subjected to renal bilateral ischemia, followed by 24, 48 and 120 h of reperfusion. At these time points, blood serum samples were collected for creatinine and urea dosages. Kidneys were harvested for histology and molecular analyses by real-time PCR. At 24 and 48 h of reperfusion, B1-/- group resulted in the lowest serum creatinine and urea levels, indicating less renal damage, which was proved by renal histology. Renal protection associated with B1-/- mice was also related with higher expression of HO-1 and lower expression of MCP-1. In conclusion, the absence of B1R had a protective role against inflammatory responses developed after renal ischemia-reperfusion injury.
Wang
Pamella Huey Mei
PH
Laboratório de Imunologia Clínica e Experimental. Division of Nephrology. Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil.
Cenedeze
Marcos Antonio
MA
Pesquero
João Bosco
JB
Pacheco-Silva
Alvaro
A
Câmara
Niels Olsen Saraiva
NO
D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics; D003401:Creatine / Q000097:blood; D015870:Gene Expression / Q000235:genetics; D051547:Heme Oxygenase-1 / Q000235:genetics; D007249:Inflammation / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D043783:Receptor, Bradykinin B1 / Q000235:genetics* / Q000502:physiology; D043782:Receptor, Bradykinin B2 / Q000235:genetics* / Q000502:physiology; D015427:Reperfusion Injury / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014508:Urea / Q000097:blood
C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D043783:Receptor, Bradykinin B1; D043782:Receptor, Bradykinin B2; D014508:Urea; D051547:Heme Oxygenase-1; D003401:Creatine