Sequence polymorphism of the mitochondrial DNA hypervariable regions I and II in 205 Singapore Malays.
Mitochondrial DNA sequences of the hypervariable regions HV1 and HV2 were analyzed in 205 unrelated ethnic Malays residing in Singapore as an initial effort to generate a database for forensic identification purposes. Sequence polymorphism was detected using PCR and direct sequencing analysis. A total of 152 haplotypes was found containing 152 polymorphisms. Out of the 152 haplotypes, 115 were observed only once and 37 types were seen in multiple individuals. The most common haplotype (16223T, 16295T, 16362C, 73G, 146C, 199C, 263G, and 315.1C) was shared by 7 (3.41%) individuals, two haplotypes were shared by 4 individuals, seven haplotypes were shared by 3 individuals, and 27 haplotypes by 2 individuals. Haplotype diversity and random match probability were estimated to be 0.9961% and 0.87%, respectively.
Wong
Hang Yee
HY
DNA Profiling Laboratory, Centre for Forensic Science, Health Sciences Authority, 11 Outram Road, Singapore 169078, Singapore. kelkate12@yahoo.com
Tang
June S W
JS
Budowle
Bruce
B
Allard
Marc W
MW
Syn
Christopher K C
CK
Tan-Siew
Wai Fun
WF
Chow
Shui Tse
ST
D022801:Complementarity Determining Regions / Q000235:genetics*; D016172:DNA Fingerprinting; D004272:DNA, Mitochondrial / Q000235:genetics*; D005006:Ethnicity / Q000208:ethnology*; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008296:Malaysia / Q000208:ethnology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D012846:Singapore
D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial