title: High amounts of 17-methylated anabolic-androgenic steroids in effervescent tablets on the dietary supplement market.
abstract: In numerous studies it has been demonstrated that several nutritional supplements contain prohormones not declared on the label. In the current study two products (effervescent tablets) containing high amounts of the 17-methylated anabolic androgenic steroids metandienone (product 1: 16.8 mg/tablet) and stanozolol (product 2: 14.5 mg/tablet) were identified. Additionally in both products norandrostenedione was detected, in product 2 with minor amounts of several other steroids. The substances identified can cause enormous health risks. In addition, the use of the analyzed tablets can lead to positive doping results for metabolites of the respective steroids in sports. This study again shows the insufficient surveillance of the production and trade of dietary supplements. Consumers should be aware of the enormous health and doping risks connected with the use of such products. For GC-MS identification of the analytes the trimethylsilyl derivatives of the steroids and the mixed N-t-butyldimethylsilyl,O-trimethylsilyl derivatives were used. The quantitation of metandienone, norandrostenedione, and stanozolol was performed using HPLC-DAD.
authors.affiliation: Centre for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Carl-Diem-Weg 6, 50933 Cologne, Germany. m.parr@biocem.dshs-koeln.de
authors.forename: Maria K
authors.initials: MK
authors.lastname: Parr
authors.forename: Hans
authors.initials: H
authors.lastname: Geyer
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hoffmann
authors.forename: Karsten
authors.initials: K
authors.lastname: Köhler
authors.forename: Ute
authors.initials: U
authors.lastname: Mareck
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Schänzer
mesh_terms: D045930:Anabolic Agents / Q000032:analysis*; D000728:Androgens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019587:Dietary Supplements / Q000032:analysis* / Q000592:standards; D004300:Doping in Sports / Q000517:prevention & control; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008696:Methandrostenolone / Q000032:analysis*; D013197:Stanozolol / Q000032:analysis*; D014297:Trimethylsilyl Compounds / Q000032:analysis / Q000737:chemistry
chemical_list: D045930:Anabolic Agents; D000728:Androgens; D014297:Trimethylsilyl Compounds; D013197:Stanozolol; D008696:Methandrostenolone