Low salt medium for lactate and ethanol production by recombinant Escherichia coli B.
Individual nutrient salts were experimentally varied to determine the minimum requirements for efficient L (+)-lactate production by recombinant strains of Escherichia coli B. Based on these results, AM1 medium was formulated with low levels of alkali metals (4.5 mM and total salts (4.2 g l(-1)). This medium was equally effective for ethanol production from xylose and lactate production from glucose with average productivities of 18-19 mmol l(-1) h(-1) for both (initial 48 h of fermentation).
Martinez
Alfredo
A
Institute of Biotechnology, National Autonomous University of Mexico, Cuernavaca, Morelos, Mexico.
Grabar
T B
TB
Shanmugam
K T
KT
Yomano
L P
LP
York
S W
SW
Ingram
L O
LO
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics; D012492:Salts / Q000008:administration & dosage*
D003470:Culture Media; D012492:Salts; D019344:Lactic Acid; D000431:Ethanol