title: C8orf4/TC-1 (thyroid cancer-1) gene expression in thyroid cancer and goiter.
abstract: BACKGROUND
  The expression of the thyroid cancer-1(TC-1) gene seems to be related with malignant transformation in the thyroid tissue.
  
  
  OBJECTIVE
  We evaluated the potential use of TC-1 gene expression as a marker of malignancy in thyroid nodules.
  
  
  METHODS
  A total of 92 frozen thyroid samples were studied, including 46 samples from thyroid nodules (19 papillary carcinomas, 1 follicular carcinoma, 24 adenomatous goiters, and 2 follicular adenomas) and 46 samples from normal surrounding thyroid tissue. Total RNA was extracted and TC-1 expression was assessed by semiquantitative Multiplex PCR. Results were verified using real-time RT-PCR in some of the samples.
  
  
  RESULTS
  Overall mean TC-1 gene expression (normalized by the ABL gene) was 1.73 +/- 1.67 (0.33-9.33). There was a significant difference (p < 0.001) between TC-1 gene expression in benign thyroid lesions (1.07 +/- 0.10) and carcinomas (2.73 +/- 0.51).
  
  
  CONCLUSIONS
  Our results suggest that TC-1 gene expression may be useful in the differential diagnosis of goiters and thyroid papillary carcinomas.
authors.affiliation: Laboratory of Molecular Genetics, São Paulo, Brazil.
authors.forename: Poliana Cristina
authors.initials: PC
authors.lastname: de Melo Martins
authors.forename: Orlando
authors.initials: O
authors.lastname: Parise Junior
authors.forename: Cora
authors.initials: C
authors.lastname: Pereira Hors
authors.forename: Roberto Elias
authors.initials: RE
authors.lastname: Villela Miguel
authors.forename: Valéria Cristina
authors.initials: VC
authors.lastname: da Costa Andrade
authors.forename: Bernardo
authors.initials: B
authors.lastname: Garicochea
mesh_terms: D000236:Adenoma / Q000000981:diagnostic imaging / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000368:Aged; D002291:Carcinoma, Papillary / Q000000981:diagnostic imaging / Q000235:genetics; D016373:DNA, Antisense / Q000235:genetics; D005260:Female; D006042:Goiter / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013964:Thyroid Neoplasms / Q000000981:diagnostic imaging / Q000235:genetics*; D014057:Tomography, X-Ray Computed
chemical_list: D016373:DNA, Antisense; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C418741:TCIM protein, human