Crystallization of ornithine acetyltransferase from yeast by counter-diffusion and preliminary X-ray study.
A study is presented on the crystallization of ornithine acetyltransferase from yeast, which catalyzes the fifth step in microbial arginine synthesis. The use of the counter-diffusion technique removes the disorder present in one dimension in crystals grown by either the batch or hanging-drop techniques. This makes the difference between useless crystals and crystals that allow successful determination of the structure of the protein. The crystals belong to space group P4, with unit-cell parameters a = b = 66.98, c = 427.09 A, and a data set was collected to 2.76 A.
Maes
Dominique
D
Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel and Vlaams Interuniversitair Instituut voor Biotechnologie, Pleinlaan 2, B-1050 Brussels, Belgium. dominique.maes@vub.ac.be
Crabeel
Marjolaine
M
Van de Weerdt
Cécile
C
Martial
Joseph
J
Peeters
Eveline
E
Charlier
Daniël
D
Decanniere
Klaas
K
Vanhee
Celine
C
Wyns
Lode
L
Zegers
Ingrid
I
D000123:Acetyltransferases / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004058:Diffusion; D005782:Gels; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012685:Sepharose
D005782:Gels; D012685:Sepharose; D000123:Acetyltransferases; C020646:glutamate acetyltransferase