title: Delayed liver regeneration in mice lacking liver serum response factor.
abstract: Various immediate early genes (IEGs) upregulated during the early process of liver regeneration are transcriptional targets of the serum response factor (SRF). We show here that the expression of SRF is rapidly induced in rodent liver after partial hepatectomy. Because the inactivation of the SRF gene in mice is embryonic lethal, the in vivo role of SRF in liver regeneration after partial hepatectomy was analyzed in mutant mice conditionally deleted for SRF in the liver. We demonstrate that SRF is not an essential factor for liver ontogenesis. However, adult mutant mice show impaired liver regeneration after partial hepatectomy, associated with a blunted upregulation of various SRF target IEGs. In conclusion, our work suggests that SRF is an early response transcription factor that may contribute to the initial phases of liver regeneration through its activation of IEGs.
authors.affiliation: Institut Cochin, Genetics and Development Department, 24 Rue du Fbg St. Jacques, 75014 Paris, France.
authors.forename: M Ujue
authors.initials: MU
authors.lastname: Latasa
authors.forename: Dominique
authors.initials: D
authors.lastname: Couton
authors.forename: Claude
authors.initials: C
authors.lastname: Charvet
authors.forename: Aurélie
authors.initials: A
authors.lastname: Lafanechère
authors.forename: Jacques-Emmanuel
authors.initials: JE
authors.lastname: Guidotti
authors.forename: Zhenlin
authors.initials: Z
authors.lastname: Li
authors.forename: David
authors.initials: D
authors.lastname: Tuil
authors.forename: Dominique
authors.initials: D
authors.lastname: Daegelen
authors.forename: Claudia
authors.initials: C
authors.lastname: Mitchell
authors.forename: Hélène
authors.initials: H
authors.lastname: Gilgenkrantz
mesh_terms: D000818:Animals; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D004247:DNA / Q000096:biosynthesis; D006498:Hepatectomy; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism* / Q000502:physiology / Q000601:surgery; D008115:Liver Regeneration / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000378:metabolism; D026362:Serum Response Factor / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D015533:Transcriptional Activation
chemical_list: D018797:Cell Cycle Proteins; D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D026362:Serum Response Factor; D004247:DNA