Detection of biomolecule by aptamer beacon.
Labeled oligodeoxyribonucleotide bearing fluorescent dyes at both ends and aptamer sequence for adenosine 5'-monophosphate (AMP) was synthesized. Fluorescence spectra of labeled aptamer were not so much different between with and without AMP. This result suggests the binding of AMP didn't cause the global structural change to the aptamer. Therefore, we used short complementary DNA (SCD) as an assistant DNA, which is an unmodified 11mer and have a complementary sequence of 5'-region of the labeled aptamer. In the presence of SCD, the fluorescence intensities decrease with increasing the concentration of AMP compared with a change in absence of SCD.
Nishihira
Akifumi
A
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan.
Ozaki
Hiroaki
H
Wakabayashi
Masayuki
M
Kuwahara
Masayasu
M
Sawai
Hiroaki
H
D000249:Adenosine Monophosphate / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000032:analysis* / Q000737:chemistry; D001483:Base Sequence; D018076:DNA, Complementary; D005453:Fluorescence; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000032:analysis* / Q000235:genetics; D013050:Spectrometry, Fluorescence
D052157:Aptamers, Nucleotide; D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; D000249:Adenosine Monophosphate