title: Effects of ultraviolet radiation on 25-hydroxyvitamin D3 synthesis in red-eared slider turtles (Trachemys scripta elegans).
abstract: OBJECTIVE
  To determine whether there are increased concentrations of 25-hydroxyvitaminn D(3) in red-eared slider turtles (Trachemys scripta elegans) after exposure to UV radiation.
  
  
  METHODS
  12 yearling turtles recently removed from aestivation.
  
  
  METHODS
  Turtles were randomly allocated to 2 groups (6 turtles/group). An initial blood sample was collected from all turtles for measurement of 25-hydroxyvitamin D(3) concentrations. Turtles of 1 group were then provided no supplemental lighting, whereas turtles of the other group were exposed to full-spectrum coil bulbs at a distance of 22.86 cm. The UV-A and UV-B radiation generated by the supplemental lighting was measured by use of a radiometer-photometer at weekly intervals. Measurements were collected 2.54 and 22.86 cm from the bulb surface. The study was continued for a 4-week period. At the end of the study, a second blood sample was collected from all turtles for measurement of 25-hydroxyvitamin D(3).
  
  
  RESULTS
  Mean +/- SD 25-hydroxyvitamin D(3) concentrations differed significantly between turtles provided supplemental UV radiation (71.7 +/- 46.9 nmol/L) and those not provided UV radiation (31.4 +/- 13.2 nmol/L).
  
  
  CONCLUSIONS
  Appropriate husbandry recommendations for raising and maintaining red-eared slider turtles should include use of sunlight that is unobstructed by UV-B filtering material or provision of an artificial source of UV-B radiation.
authors.affiliation: Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70810, USA.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Acierno
authors.forename: Mark A
authors.initials: MA
authors.lastname: Mitchell
authors.forename: Marlana K
authors.initials: MK
authors.lastname: Roundtree
authors.forename: Trevor T
authors.initials: TT
authors.lastname: Zachariah
mesh_terms: D000818:Animals; D002112:Calcifediol / Q000097:blood / Q000528:radiation effects*; D011897:Random Allocation; D014426:Turtles / Q000502:physiology*; D014466:Ultraviolet Rays
chemical_list: D002112:Calcifediol