title: Electricity from low-level H2 in still air--an ultimate test for an oxygen tolerant hydrogenase.
abstract: We demonstrate an extreme test of O(2) tolerance for a biological hydrogen-cycling catalyst: the generation of electricity from just 3% H(2) released into still, ambient air using an open fuel cell comprising an anode modified with the unusual hydrogenase from Ralstonia metallidurans CH34, that oxidizes trace H(2) in atmospheric O(2), connected via a film of electrolyte to a cathode modified with the fungal O(2) reductase, laccase.
authors.affiliation: Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, UK.
authors.forename: Kylie A
authors.initials: KA
authors.lastname: Vincent
authors.forename: James A
authors.initials: JA
authors.lastname: Cracknell
authors.forename: Jeremy R
authors.initials: JR
authors.lastname: Clark
authors.forename: Marcus
authors.initials: M
authors.lastname: Ludwig
authors.forename: Oliver
authors.initials: O
authors.lastname: Lenz
authors.forename: Bärbel
authors.initials: B
authors.lastname: Friedrich
authors.forename: Fraser A
authors.initials: FA
authors.lastname: Armstrong
mesh_terms: D000388:Air; D001674:Bioelectric Energy Sources; D004560:Electricity; D004563:Electrochemistry; D006859:Hydrogen / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006864:Hydrogenase / Q000737:chemistry*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D043367:Ralstonia / Q000201:enzymology; D017382:Reactive Oxygen Species / Q000737:chemistry
chemical_list: D017382:Reactive Oxygen Species; D006859:Hydrogen; D006861:Hydrogen Peroxide; D006864:Hydrogenase; D010100:Oxygen