Histocompatible embryonic stem cells by parthenogenesis.
Genetically matched pluripotent embryonic stem (ES) cells generated via nuclear transfer or parthenogenesis (pES cells) are a potential source of histocompatible cells and tissues for transplantation. After parthenogenetic activation of murine oocytes and interruption of meiosis I or II, we isolated and genotyped pES cells and characterized those that carried the full complement of major histocompatibility complex (MHC) antigens of the oocyte donor. Differentiated tissues from these pES cells engrafted in immunocompetent MHC-matched mouse recipients, demonstrating that selected pES cells can serve as a source of histocompatible tissues for transplantation.
Kim
Kitai
K
Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA.
Lerou
Paul
P
Yabuuchi
Akiko
A
Lengerke
Claudia
C
Ng
Kitwa
K
West
Jason
J
Kirby
Andrew
A
Daly
Mark J
MJ
Daley
George Q
GQ
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D020090:Chromosome Segregation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D005260:Female; D005838:Genotype; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology*; D006579:Heterozygote; D006648:Histocompatibility; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D008285:Major Histocompatibility Complex; D008540:Meiosis; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009865:Oocytes / Q000166:cytology / Q000276:immunology; D010312:Parthenogenesis; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D033581:Stem Cell Transplantation
D006183:H-2 Antigens; C489629:H-2A(b) antigen, mouse; C024755:H-2K(K) antigen; D000949:Histocompatibility Antigens Class II