title: [Adenovirus-mediated overexpression of KAI1 suppresses sphingosine kinase activation and metastasis of pancreatic carcinoma cells].
abstract: OBJECTIVE
  To investigate influence of KAI1 expression on the pancreatic carcinoma cell migration and its mechanisms.
  
  
  METHODS
  We construct an adenoviral vector carrying the full length cDNA of KAI1 (Ad-KAI1). The Ad-KAI1 virus was packed and amplified in 293 cells and was purified by Adeno-X Virus Purification Kit. The adenovirus-mediated KAI1 expression in human pancreatic carcinoma cell line (PANC I) was detected by flow cytometric analysis. Sphingosine kinase (SPK) activation was determined by [gamma-(32)P] incorporation. And cell migration was assayed by using transwell.
  
  
  RESULTS
  We constructed Ad-KAI1 vector successfully. The 86.3% of pancreatic cancer cells PANC I were identified and infected by Ad-KAI1 constructed by us. At same time, we found out higher concentration of SPK in the PANC I cancer cells without infection of Ad-KAI1 using RT-PCR. Overexpression of KAI1 significantly inhibited the activity of SPK and the migration ability of the PANC I cells, which was induced by activity of SPK.
  
  
  CONCLUSIONS
  Adenovirus-mediated overexpression of KAI1 suppresses sphingosine kinase activation and migration of pancreatic carcinoma cells. This research is helpful for elucidating the anti-metastasis mechanisms KAI1 in pancreatic carcinoma cells.
authors.affiliation: Department of Gastroenterology, Shenyang General Hospital of PLA, Shenyang 110016, China. gxzsw@pub.sy.ln.cn
authors.forename: Xiao-zhong
authors.initials: XZ
authors.lastname: Guo
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Zhang
authors.forename: Li-sheng
authors.initials: LS
authors.lastname: Wang
authors.forename: Zhuo-zhuang
authors.initials: ZZ
authors.lastname: Lu
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Tian
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D045744:Cell Line, Tumor; D006801:Humans; D051861:Kangai-1 Protein / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D014161:Transduction, Genetic; D014162:Transfection; D000099157:Sphingosine Kinase
chemical_list: D051861:Kangai-1 Protein; D017853:Phosphotransferases (Alcohol Group Acceptor); D000099157:Sphingosine Kinase