Raloxifene covalently bonded to titanium implants by interfacing with (3-aminopropyl)-triethoxysilane affects osteoblast-like cell gene expression.
Since Raloxifene, a drug used in osteoporosis therapy, inhibits the osteoclast functions but not osteoblast functions, it could improve the recovery during implant surgery. This preliminary report describes a simple method to link, through a covalent bond, Raloxifene to titanium by interfacing with (3-aminopropyl)-Triethoxysilane as assessed by the IR-FT and SEM. To evaluate the biological response of osteoblast-like cells to this implant, we compared expression gene profiling of cell cultures on Raloxifene conjugated implant and normal implant by DNA microarray. By using DNA microarrays containing 19,200 genes, we identified differently expressed genes in osteoblast-like cell line (MG-63). Surface Raloxifene conjugated implants have been shown to have a relevant importance in modifying cell response. This result could be an interesting starting point for the use of an immediate functional loading of implants.
Bambini
F
F
Università Politecnica delle Marche, Istituto di Scienze Odontostomatologiche, Ancona, Italy.
Greci
L
L
Memè
L
L
Santarelli
A
A
Carinci
F
F
Pezzetti
F
F
Procaccini
M
M
Lo Muzio
L
L
D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D008855:Microscopy, Electron, Scanning; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D011437:Propylamines; D019736:Prostheses and Implants; D020849:Raloxifene Hydrochloride / Q000737:chemistry / Q000494:pharmacology*; D012821:Silanes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000737:chemistry*
D011437:Propylamines; D012821:Silanes; D020849:Raloxifene Hydrochloride; D014025:Titanium; C477625:amino-propyl-triethoxysilane