title: [Prevalence and clinical characteristics of the A to G variant at position 12026 of the mitochondrial ND4 gene in familial diabetes mellitus in Chinese population].
abstract: OBJECTIVE
  To assess the prevalence of the A to G variant at nucleotide 12026 (mt12026) of the mitochondrial NADH-dehydrogenase subunit 4 (ND4) gene in familial diabetes mellitus in Chinese population.
  
  
  METHODS
  The authors screened 770 randomly selected, unrelated probands of diabetic pedigrees, and 309 controls with normal glucose tolerance for the variant by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique and PCR-direct-sequencing.
  
  
  RESULTS
  The mt12026 A --> G variant was detected in 28 diabetic patients (3.63%) and 9 controls (2.91%). The frequency of the variant mt12026 A --> G was not statistically different between diabetic patients and controls. Moreover, clinical characteristics such as age, body mass index (BMI), and insulin resistant index were not different between diabetic patients with and without the mt12026 mutation.
  
  
  CONCLUSIONS
  The mt12026 A --> G variant is a mitochondrial gene polymorphism in Chinese population, and it is unlikely that the mutation is in itself the cause of diabetes.
authors.affiliation: Shanghai Diabetes Institute, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, 200233, P. R. China.
authors.forename: Sui-jun
authors.initials: SJ
authors.lastname: Wang
authors.forename: Song-hua
authors.initials: SH
authors.lastname: Wu
authors.forename: Tai-shan
authors.initials: TS
authors.lastname: Zheng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yang
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Lu
mesh_terms: D044466:Asian People / Q000235:genetics*; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D002681:China; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D003920:Diabetes Mellitus / Q000097:blood / Q000208:ethnology / Q000235:genetics*; D005192:Family Health; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D009245:NADH Dehydrogenase / Q000235:genetics*; D017354:Point Mutation; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D001786:Blood Glucose; D004272:DNA, Mitochondrial; C098483:NADH dehydrogenase subunit 4; D009245:NADH Dehydrogenase