title: Testing of bacteria isolated from HIV/AIDS patients in experimental models.
abstract: OBJECTIVE
  Bacteria purified from the intestinal tract of HIV/AIDS patients were tested for the capacity to be internalised by cells of the HL-60 cell line. Secondly, the bacteria have been applied to the rabbit's colon in order to test their pathogenic ability.
  
  
  RESULTS
  The ability of the bacteria to be internalised by HL-60 cells was found to be very expressive. For a more complex biological characterisation of internalised bacteria, these were applied in 6-day intervals per rectum to NZB x CA rabbits during 8 months. The administered bacteria were detected by dot blot hybridisation using HIV-1 PCR probes in the rabbits' intestinal tract after 2 months. No histological and pathological changes were recorded in the gastrointestinal epithelial cells of rabbits.
  
  
  CONCLUSIONS
  The applied bacteria of HIV/AIDS patients were not colonised in the rabbit's colon and disappeared after 2 months. Differences between HL-60 and rabbit model are discussed.
authors.affiliation: Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovakia. vladimir.zajac@savba.sk
authors.forename: Vladimír
authors.initials: V
authors.lastname: Zajac
authors.forename: Michal
authors.initials: M
authors.lastname: Mego
authors.forename: David
authors.initials: D
authors.lastname: Martinický
authors.forename: Viola
authors.initials: V
authors.lastname: Stevurková
authors.forename: Sona
authors.initials: S
authors.lastname: Cierniková
authors.forename: Eduard
authors.initials: E
authors.lastname: Ujházy
authors.forename: Andrej
authors.initials: A
authors.lastname: Gajdosík
authors.forename: Alena
authors.initials: A
authors.lastname: Gajdosíková
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000818:Animals; D001419:Bacteria / Q000302:isolation & purification* / Q000472:pathogenicity; D003106:Colon / Q000382:microbiology* / Q000502:physiology; D015169:Colony Count, Microbial; D004705:Endocytosis; D005260:Female; D015497:HIV-1; D018922:HL-60 Cells; D006801:Humans; D008297:Male; D050296:Microbial Viability; D008954:Models, Biological; D011817:Rabbits