title: Chiral separation of beta-blockers after derivatization with a new chiral derivatization agent, GATC.
abstract: A new chiral derivatization agent with sugar moiety, 2,3,4,6-tetra-O-acetyl-beta-D-galactopyranosyl isothiocyanate (GATC) was synthesized. Several beta-blockers were investigated for the possible separation of the enantiomers by reversed-phase HPLC after derivatization with this new chiral derivatization agent (GATC). GATC was reacted readily with beta-blockers at room temperature and the reaction mixture could directly be injected into the HPLC system. The corresponding diastereomers were well resolved on an ODS column with acetonitrile-ammonium acetate buffer as a mobile phase and monitored at UV 254 nm. The optimization of the derivatization procedure (concentration of GATC, reaction temperature and time) and HPLC conditions (pH and ionic strength of mobile phase) were investigated and compared with GITC.
authors.affiliation: College of Pharmacy, Kangwon National University, Chunchon 200-701, Korea.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Ko
authors.forename: Dae Hong
authors.initials: DH
authors.lastname: Shin
authors.forename: Joung Weon
authors.initials: JW
authors.lastname: Oh
authors.forename: Workaferhaw Shibru
authors.initials: WS
authors.lastname: Asegahegn
authors.forename: Kyeong Ho
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000085:Acetates; D000097:Acetonitriles; D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000302:isolation & purification*; D000588:Amines / Q000737:chemistry; D000605:Amino Alcohols / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D017879:Isothiocyanates / Q000737:chemistry*; D009005:Monosaccharides / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: C515643:2,3,4,6-tetra-O-acetyl-beta-D-galactopyranosyl isothiocyanate; D000085:Acetates; D000097:Acetonitriles; D000319:Adrenergic beta-Antagonists; D000588:Amines; D000605:Amino Alcohols; D007202:Indicators and Reagents; D017879:Isothiocyanates; D009005:Monosaccharides; C018824:ammonium acetate