title: Two novel nuclear genes, OsSIG5 and OsSIG6, encoding potential plastid sigma factors of RNA polymerase in rice: tissue-specific and light-responsive gene expression.
abstract: Two novel nuclear genes, OsSIG5 and OsSIG6, encoding potential plastid sigma factors of RNA polymerase (RNAP) were identified in Oryza sativa. The deduced amino acid sequences contain conserved regions, regions 1.2-4.2, and a novel region A/B at the N-terminus. Tissue-specific and light-responsive transcripts of OsSIG5 and OsSIG6 were observed. The N-terminal region of OsSig5 conferred import of green fluorescent protein into the chloroplast. Specific transcripts of rice psbA were synthesized in vitro by reconstituted OsSig5-RNAP holoenzymes. These results indicated that OsSig5 is a plastid sigma factor. This is the first report of the Sig5-type sigma factor in crops.
authors.affiliation: Laboratory of Molecular Genetics, Collage of Agriculture, Ibaraki University, Ami, Inashiki, Ibaraki, 300-0393 Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Kubota
authors.forename: Akio
authors.initials: A
authors.lastname: Miyao
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Hirochika
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Tozawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yasuda
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Tsunoyama
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Niwa
authors.forename: Sousuke
authors.initials: S
authors.lastname: Imamura
authors.forename: Makoto
authors.initials: M
authors.lastname: Shirai
authors.forename: Munehiko
authors.initials: M
authors.lastname: Asayama
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics; D018547:Plant Stems / Q000235:genetics; D018087:Plastids / Q000235:genetics*; D012808:Sigma Factor / Q000737:chemistry / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D012808:Sigma Factor; D012321:DNA-Directed RNA Polymerases