Screening of DNA aptamers against multiple proteins in tissue.
We report the development of a novel screening method for DNA aptamers against multiple proteins in the target tissue. Although a purified single protein is generally used in screening of aptamers at present, such a simultaneous selection would be very advantageous in efficiency and selectivity. We first carried out the screening in situ and it was suggested that aptamers against particular target proteins were enriched. We also developed another novel screening method named Aptamer Blotting based on protein separation by PAGE and its visualization with fluorescein labeled aptamers and it was demonstrated that this method allowed the simultaneous selection of specific aptamers against multiple proteins.
Noma
Takahisa
T
Department of Biotechnology & Life Science, Tokyo University of Agriculture & Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Ikebukuro
Kazunori
K
Sode
Koji
K
Ohkubo
Takuya
T
Sakasegawa
Yuji
Y
Hachiya
Naomi
N
Kaneko
Kiyotoshi
K
D000818:Animals; D052157:Aptamers, Nucleotide / Q000032:analysis*; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D015151:Immunoblotting / Q000379:methods*; D015336:Molecular Probe Techniques; D018482:Muscle, Skeletal / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D052156:SELEX Aptamer Technique
D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D011506:Proteins; D016650:Fluorescein-5-isothiocyanate