title: Perfusion flow bioreactor for 3D in situ imaging: investigating cell/biomaterials interactions.
abstract: The capability to image real time cell/material interactions in a three-dimensional (3D) culture environment will aid in the advancement of tissue engineering. This paper describes a perfusion flow bioreactor designed to hold tissue engineering scaffolds and allow for in situ imaging using an upright microscope. The bioreactor can hold a scaffold of desirable thickness for implantation (>2 mm). Coupling 3D culture and perfusion flow leads to the creation of a more biomimetic environment. We examined the ability of the bioreactor to maintain cell viability outside of an incubator environment (temperature and pH stability), investigated the flow features of the system (flow induced shear stress), and determined the image quality in order to perform time-lapsed imaging of two-dimensional (2D) and 3D cell culture. In situ imaging was performed on 2D and 3D, culture samples and cell viability was measured under perfusion flow (2.5 mL/min, 0.016 Pa). The visualization of cell response to their environment, in real time, will help to further elucidate the influences of biomaterial surface features, scaffold architectures, and the influence of flow induced shear on cell response and growth of new tissue.
authors.affiliation: Polymers Division, National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, Maryland 20899, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Stephens
authors.forename: J A
authors.initials: JA
authors.lastname: Cooper
authors.forename: F R
authors.initials: FR
authors.lastname: Phelan
authors.forename: J P
authors.initials: JP
authors.lastname: Dunkers
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D019149:Bioreactors; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival; D002999:Clone Cells; D003470:Culture Media; D021621:Imaging, Three-Dimensional; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D010477:Perfusion; D011091:Polyesters / Q000378:metabolism; D013379:Substrate Specificity; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media; D011091:Polyesters; C016240:polycaprolactone