The expression of FHIT, PCNA and EGFR in benign and malignant breast lesions.
Immunohistochemical staining for FHIT and PCNA proteins was carried out in 451 breast lesions showing nonproliferative benign breast disease (BBD) (n=263), proliferative BBD without atypia (n=128), proliferative BBD with atypia (n=11), carcinoma in situ (n=15) or invasive carcinoma (n=34) and for EGFR protein in a subset of 71 of these cases. FHIT underexpression was not detected in nonproliferative lesions, but occurred in 2% of proliferative BBD without atypia, 10% proliferative BBD with atypia, 27% of carcinoma in situ and 41% of invasive carcinoma, which suggests that it could be useful in assessing those carcinoma in situ lesions (ductal, DCIS and lobular, LCIS) that are more likely to progress to malignancy. Preliminary microarray comparisons on DCIS and invasive carcinoma samples dissected from formalin-fixed paraffin sections showed a consistent downregulation of two previously identified FHIT-related genes, caspase 1 and BRCA1 in lesions underexpressing FHIT.
Terry
G
G
Department of Epidemiology, Mathematics and Statistics, Cancer Research UK, Queen Mary University of London, Wolfson Institute, Charterhouse Square, London ECIM 6BQ, UK.
Ho
L
L
Londesborough
P
P
Duggan
C
C
Hanby
A
A
Cuzick
J
J
D017766:Acid Anhydride Hydrolases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D001941:Breast Diseases / Q000378:metabolism* / Q000473:pathology; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000378:metabolism* / Q000473:pathology; D066246:ErbB Receptors / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D012313:RNA / Q000235:genetics; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D066246:ErbB Receptors; D009363:Neoplasm Proteins; D018809:Proliferating Cell Nuclear Antigen; D012313:RNA; D000099170:Bis(5'-adenosyl)triphosphatase