Telomere shortening in human HL60 cells by treatment with deoxyguanosine analogs.
Telomerase is a cellular endogenous reverse transcriptase thought to play an important role in the maintenance of telomere length. We investigated the effects of 3'-azido-2',3'-dideoxyguanosine (AZddG) and carbocyclic oxetanocin G (C.OXT-G), of which the triphosphate derivatives AZddGTP and C.OXT-GTP show potent telomerase inhibition, on telomere length of human HL60 cells in culture. Although AZddG caused more significant telomere shortening than C.OXT-G, only a slight decrease of cell growth rate was observed.
Yamaguchi
Toyofumi
T
Department of Biological Sciences, Teikyo University of Science and Technology, Uenohara, Yamanashi 409-0193, Japan.
Takahashi
Hazuki
H
Harada
Yoko
Y
Yoshikawa
Masato
M
Iyono
Saori
S
Inomata
Motoko
M
Maruyama
Tokumi
T
Saneyoshi
Mineo
M
D002455:Cell Division / Q000187:drug effects; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D016615:Telomere / Q000378:metabolism*
D003849:Deoxyguanosine