Preparative isolation and purification of chemical constituents from the root of Adenophora tetraphlla by high-speed counter-current chromatography with evaporative light scattering detection.
Preparative high-speed counter-current chromatography (HSCCC), as a continuous liquid-liquid partition chromatography with no solid support matrix, combined with evaporative light scattering detection (ELSD) was employed for systematic separation and purification of non-chromophoric chemical components from Chinese medicinal herb Adenophora tetraphlla (Thunb.), Fisch. Nine compounds, including alpha-spinasterol, beta-sitosterol, nonacosan-10-ol, 24-methylene cycloartanol, lupenone, 3-O-palmitoyl-beta-sitosterol, 3-O-beta-d-glucose-beta-sitosterol, eicosanoic acid and an unknown compound, were obtained. The compounds were all above 95% determined by high-performance liquid chromatography (HPLC)-ELSD, and their structures were identified by (1)H NMR and chemical ionization mass spectroscopy (CI-MS). The results demonstrate that HSCCC coupled with ELSD is a feasible and efficient technique for systematic isolation of non-chromophoric components from traditional medicinal herbs.
Yao
Shun
S
Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China.
Liu
Renming
R
Huang
Xuefeng
X
Kong
Lingyi
L
D029422:Campanulaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution; D008027:Light; D010840:Phytosterols / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D012855:Sitosterols / Q000302:isolation & purification; D013265:Stigmasterol / Q000031:analogs & derivatives / Q000302:isolation & purification; D014315:Triterpenes / Q000302:isolation & purification; D000094042:Lupanes
D010840:Phytosterols; D012855:Sitosterols; D013265:Stigmasterol; D014315:Triterpenes; C468854:10-nonacosanol; C470592:lupenone; D000094042:Lupanes; C031312:spinasterol; C066041:24-methylene cycloartanol; C025473:gamma-sitosterol