Suppression of RUNX1 by siRNA in megakaryocytic UT-7/GM cells.
RUNX1 is a transcription factor that plays critical roles in hematopoietic proliferation and differentiation. Megakaryocyte is a precursor cell of platelets. Several reports have implied that RUNX1 is important in megakaryocytic differentiation and proliferation. However, the mechanism is not well understood. In this study, we employed a megakaryocytic cell line UT-7/GM and suppressed the RUNX1 gene expression by siRNA. Knocking down of RUNX1 induced the increase of megakaryocyte-specific gene expression and down-regulation of polyploidization. RUNX1 overexpression decreased the PF4 and GPIIb promoter activities. These results suggest that RUNX1 promotes proliferation of megakaryocytic cell line but not megakaryocytic gene expression in UT-7/GM cells.
Okada
Yoshiaki
Y
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka, Japan.
Nagai
Ryohei
R
Matsuura
Eri
E
Hoshika
Yusuke
Y
Nakata
Emi
E
Nagura
Hironori
H
Watanabe
Ayako
A
Komatsu
Norio
N
Doi
Takefumi
T
D049109:Cell Proliferation; D050676:Core Binding Factor Alpha 2 Subunit / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology / Q000378:metabolism*; D011123:Polyploidy; D034622:RNA Interference; D034741:RNA, Small Interfering; D015854:Up-Regulation
D050676:Core Binding Factor Alpha 2 Subunit; D034741:RNA, Small Interfering; C493728:RUNX1 protein, human