title: Therapeutic inhibition of yellow head virus multiplication in infected shrimps by YHV-protease dsRNA.
abstract: Yellow head virus (YHV) is an invertebrate nidovirus which causes a severe mortality in cultured Penaeus monodon. The mortality may be prevented by prior treatment of shrimps with YHV-protease dsRNA. Whether the YHV infected shrimp might be cured by the dsRNA remains to be investigated. P. monodon injected with 10(-6) YHV showed a high virus replication and mortality within 2 days. Injection of 25 microg YHV-protease dsRNA at 3, 6, 12 or 24 h post YHV infection showed a strong inhibition of YHV replication up to 12 h. Unrelated dsRNA-GFP showed no inhibition, indicating that the inhibition was nucleic acid sequence specific through RNAi pathway. Shrimp mortality could be prevented at 3h post YHV infection by the dsRNA, but not at 24 h. These results demonstrate that YHV-protease dsRNA gives therapeutic effect and pave the way to develop a cure for YHV-infected shrimps.
authors.affiliation: The Institute of Molecular Biology and Genetics, Mahidol University, Salaya, Nakhonpathom 73170, Thailand. mbwtr@mahidol.ac.th
authors.forename: Witoon
authors.initials: W
authors.lastname: Tirasophon
authors.forename: Supansa
authors.initials: S
authors.lastname: Yodmuang
authors.forename: Wanlop
authors.initials: W
authors.lastname: Chinnirunvong
authors.forename: Nongluk
authors.initials: N
authors.lastname: Plongthongkum
authors.forename: Sakol
authors.initials: S
authors.lastname: Panyim
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D033561:Penaeidae / Q000821:virology*; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000235:genetics; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics / Q000494:pharmacology*; D012367:RNA, Viral / Q000037:antagonists & inhibitors* / Q000235:genetics; D046828:Roniviridae / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D010447:Peptide Hydrolases