A nanocatalyst-based assay for proteins: DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle labels.
This communication reports a nanocatalyst-based electrochemical assay for proteins. Ultrasensitive detection has been achieved by signal amplification combined with noise reduction: the signal is amplified both by the catalytic reduction of p-nitrophenol to p-aminophenol by gold-nanocatalyst labels and by the chemical reduction of p-quinone imine to p-aminophenol by NaBH4; the noise is reduced by employing an indium tin oxide electrode modified with a ferrocenyl-tethered dendrimer and a hydrophilic immunosensing layer.
Das
Jagotamoy
J
Department of Chemistry, Pusan National University, Busan 609-735, Korea.
Aziz
Md Abdul
MA
Yang
Haesik
H
D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D009596:Nitrophenols / Q000737:chemistry*; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry*
D009596:Nitrophenols; D011506:Proteins; D006046:Gold; D004247:DNA; C024836:4-nitrophenol