Mechanistic model for adsorption of immunoglobulin on hydroxyapatite.
A model is developed for correlating the adsorption of protein to hydroxyapatite in terms of pH, phosphate and imidazole. It provides a basis for manipulating the chromatography conditions so as to obtain the best possible fractionation. The adsorption mechanism is apparently a function of two calcium affinity interactions and cation exchange. We used immunoglobulins and a fusion protein with or without a poly(6)histidine tag for our investigation. The results are presented and discussed in the present study.
Ng
Paul K
PK
Process Applications, Process Chromatography Division, Life Sciences Group, Bio-Rad Laboratories, Hercules, CA 94547, USA. paul_ng@bio-rad.com <paul_ng@bio-rad.com>
He
Jie
J
Gagnon
Pete
P
D000327:Adsorption / Q000187:drug effects*; D001665:Binding Sites; D017886:Durapatite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000494:pharmacology*; D007136:Immunoglobulins / Q000737:chemistry / Q000378:metabolism*; D010710:Phosphates / Q000737:chemistry
D007093:Imidazoles; D007136:Immunoglobulins; D010710:Phosphates; C029899:imidazole; D017886:Durapatite