Expression of cyclooxygenase-2 and 5-lipoxygenase in nasal polyps associated with interleukin-4 promoter polymorphism -590.
OBJECTIVE
To evaluate expression of cyclooxygenase (COX)-2 and 5-lipoxygenase (5-LO) associated with interleukin (IL)-4 promoter polymorphism -590 in nasal polyp tissues.


METHODS
A prospective controlled study. A venous blood sample was taken to determine the genotype in 61 nasal polyp subjects. The C-590T variant was determined by the polymerase chain reaction-restriction fragment length polymorphism method. The expression of 5-LO and COX-2 was determined with immunohistochemical staining in 37 nasal polyp tissues associated with genotype.


RESULTS
The genotype frequencies at position -590 of the IL-4 gene in the patients with nasal polyp were C/C (8.20%), C/T (40.98%), and T/T (50.82%). There was no significantly increased expression of COX-2 among genotypes. The 5-LO expression was significantly increased in C/C compared with C/T and T/T.


CONCLUSIONS
We suggested that the IL-4 promoter polymorphism -590 C/C is associated with the expression of 5-LO in the patients with nasal polyp.
Park
Seong Kook
SK
Department of Otorhinolaryngology-Head and Neck Surgery, Inje University, College of Medicine, Pusan Paik Hospital, Busan, Korea. sinus4@pusanpaik.or.kr
Heo
Kyung Wook
KW
Jung
Hyun
H
Yea
Sung Su
SS
Yang
Young Il
YI
D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D016022:Case-Control Studies; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D015847:Interleukin-4 / Q000235:genetics*; D009298:Nasal Polyps / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D011446:Prospective Studies
D015847:Interleukin-4; D001094:Arachidonate 5-Lipoxygenase; D051546:Cyclooxygenase 2