title: Desmoid and fibroma tumors differently respond to TGFbeta(1) stimulus and ECM macromolecule accumulation.
abstract: Desmoid and fibroma tumours are characterized by cell proliferation, glycosaminoglycan and collagen fibre accumulation, high levels of transforming growth factor beta(1) (TGFbeta(1)) and different patterns of tissue infiltration. TGFbeta(1) is related to extracellular matrix (ECM) composition which, in turn, regulates cell functions and cell migration. In this study we report changes in cell proliferation, glycosaminoglycan (GAG) and collagen synthesis, TGFbeta(1) mRNA expression and fibronectin levels in normal, desmoid and fibroma fibroblast cultures before and after TGFbeta(1) stimulation. Our data showed cell proliferation, GAG and collagen synthesis, transforming growth factor beta(1) mRNA expression and fibronectin levels were significantly higher in desmoid than in fibroma cultures. TGFbeta(1) treatment had no effect on cell proliferation, but increased TGFbeta(1) mRNA expression, GAG, fibronectin and collagen synthesis in desmoid and fibroma fibroblasts. Its effects were more marked in desmoid cells. Fibronectin favours cell migration, while changes in GAG composition alter cell behaviour and ECM organization. In conclusion our data suggest that the different patterns of infiltration in desmoid and fibroma tumours are due to changes in ECM components and cell-ECM interactions which can be ascribed to altered TGFbeta(1) mRNA expression and TGFbeta(1) activity.
authors.affiliation: Department of Experimental Medicine and Biochemical Science, University of Perugia, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Locci
authors.forename: Chiara
authors.initials: C
authors.lastname: Balducci
authors.forename: Cinzia
authors.initials: C
authors.lastname: Lilli
authors.forename: Lorella
authors.initials: L
authors.lastname: Marinucci
authors.forename: Ennio
authors.initials: E
authors.lastname: Becchetti
authors.forename: Claudia
authors.initials: C
authors.lastname: Dolci
authors.forename: Carla
authors.initials: C
authors.lastname: Calastrini
authors.forename: Eleonora
authors.initials: E
authors.lastname: Lumare
authors.forename: Giordano
authors.initials: G
authors.lastname: Stabellini
mesh_terms: D015152:Blotting, Northern; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D003094:Collagen / Q000096:biosynthesis; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D018222:Desmoid Tumors / Q000378:metabolism* / Q000503:physiopathology; D005353:Fibronectins / Q000378:metabolism; D015870:Gene Expression; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006801:Humans; D007889:Leiomyoma / Q000378:metabolism* / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D005353:Fibronectins; D006025:Glycosaminoglycans; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D003094:Collagen