Enzymatic preparation of metabolic intermediates, 3-dehydroquinate and 3-dehydroshikimate, in the shikimate pathway.
A method for enzymatic preparation of 3-dehydroquinate and 3-dehydroshikimate in the shikimate pathway was established by controlling the enzyme activity of 3-dehydroquinate dehydratase. When quinate was incubated with the membrane fraction of acetic acid bacteria at pH 5.0, 3-dehydroquinate was formed as the predominant product. 3-Dehydroshikimate was the sole product when incubated at pH 8.0. Mutual separation of the metabolic intermediates was also exemplified.
Adachi
Osao
O
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University. osao@yamaguchi-u.ac.jp
Ano
Yoshitaka
Y
Toyama
Hirohide
H
Matsushita
Kazunobu
K
D002853:Chromatography, Liquid; D010084:Oxidation-Reduction; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D012765:Shikimic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
C474477:3-dehydroquinic acid; D011801:Quinic Acid; C096387:3-dehydroshikimate; D012765:Shikimic Acid