title: Faster and easier chromatin immunoprecipitation assay with high sensitivity.
abstract: Chromatin immunoprecipitation (ChIP) assays are widely used to investigate where chromatin-binding proteins bind to the genome. The standard assay is very time consuming. We have developed a rapid ChIP assay in which the immunoprecipitates serve directly as PCR templates. This assay eliminates the step to reverse the crosslinking, shortening the assay by 1 day. It also requires a less immunoprecipitating antibody, permits many samples to be tested simultaneously, and is more sensitive than the standard ChIP assay.
authors.affiliation: Venture Laboratory, Kyoto Institute of Technology, Kyoto, Japan. hkohzaki@virus.kyoto-u.ac.jp
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Kohzaki
authors.forename: Yota
authors.initials: Y
authors.lastname: Murakami
mesh_terms: D047369:Chromatin Immunoprecipitation / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000032:analysis; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins