Electrochemical study on behavior of EuMo2 complex and its interaction with DNA.
The electrochemical behavior of complex EuMo2 (Mo = Morin, 2',3,4'5,7-pentahydroxyflavone) and its interactions with calf thymus DNA were studied using cyclic voltammetry (CV) and double potential step chronocoulometry (DPSCC) at glass carbon electrode (GCE) and DNA modified GCE, respectively. Information such as diffusion coefficient (D), rate constant (ks) of EuMo2 and intrinsic binding constant (K), binding numbers (n) of bound species per DNA (bp) were obtained. EuMo2 can bind to DNA, and the binding mode is intercalation. By nonlinear fitting with Langmuir equation, a K of 1.02 x 10(6) M-1 and an n of 1 were obtained.
Li
Zhifeng
Z
School of life Science and Chemistry, Tianshui Normal University, Tianshui, PR China.
Kang
Jingwan
J
Lu
Xiaoquan
X
D000818:Animals; D002417:Cattle; D003217:Conductometry; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D007364:Intercalating Agents / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*
D007364:Intercalating Agents; D009942:Organometallic Compounds; C518806:bis(2',3,4',5,7-pentahydroxyflavone)europium(III); D004247:DNA; C427203:calf thymus DNA