New designs for DNA bases: expanded DNAs and oligofluorosides.
Properties and applications of two new classes of DNA base replacements are described. The first class is size-expanded DNA bases; these are benzo-homologous versions of A, C, T, G that yield base pairs 2.4 Angstroms larger than natural pairs. New studies have explored the ability of expanded DNA base pairs to be replicated by DNA polymerase enzymes. The second class of DNA-like molecules described here are "oligodeoxyfluorosides" (ODFs), which are deoxyribose-phosphate oligomers in which all nucleobases are replaced by hydrocarbon and heterocyclic fluorophores. These compounds have been prepared in large libraries and have been screened for unusual fluorescence and sensing properties.
Kool
Eric T
ET
Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
Lu
Haige
H
Kim
Su Jeong
SJ
Tan
Samuel
S
Wilson
James N
JN
Gao
Jianmin
J
Liu
Haibo
H
D000225:Adenine / Q000031:analogs & derivatives; D020029:Base Pairing; D003596:Cytosine / Q000031:analogs & derivatives; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006147:Guanine / Q000031:analogs & derivatives; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry; D013941:Thymine / Q000031:analogs & derivatives
D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D006147:Guanine; D003596:Cytosine; D004247:DNA; D000225:Adenine; D013941:Thymine