Effect of Toki-shakuyaku-san on acetylcholine level and blood flow in dorsal hippocampus of intact and twice-repeated ischemic rats.
The effects of Toki-shakuyaku-san (TSS) ingredients on acetylcholine (ACh) release in the dorsal hippocampus (DH) were investigated in intact and twice-repeated ischemic rats using in vivo microdialysis-HPLC. Moreover, the effect of TSS on blood flow was investigated in intact rats using laser Doppler flowmetry. TSS at 300 mg/kg p.o. increased ACh and blood flow after 40 min in intact rats. TSS also increased ACh in ischemic rats but to a lesser extent than in intact rats. These results could suggest that TSS-increased ACh and blood flow in DH may contribute in the cognition improving property of TSS.
Hatip-Al-Khatib
Izzettin
I
Department of Pharmacology, Division of Internal Medicine, Faculty of Medicine, Pamukkale University, Kinikli, Denizli 20070, Turkey. izhatip@yahoo.com
Hatip
Funda Bölukbaşi
FB
Yoshimitsu
Yoshitaka
Y
Iwasaki
Katsunori
K
Egashira
Nobuaki
N
Liu
An-Xin
AX
Mishima
Kenichi
K
Fujiwara
Michihiro
M
D000109:Acetylcholine / Q000096:biosynthesis*; D000818:Animals; D001783:Blood Flow Velocity; D002545:Brain Ischemia; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006624:Hippocampus / Q000098:blood supply* / Q000378:metabolism*; D017078:Laser-Doppler Flowmetry; D008297:Male; D008517:Phytotherapy; D010946:Plants, Medicinal; D011673:Pulsatile Flow; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; C413220:toki-shakuyaku-san; D000109:Acetylcholine