title: [Effect of shRNA-mediated survivin gene silencing on apoptosis and proliferation of leukemia cell line].
abstract: OBJECTIVE
  To transfect a short hairpin RNA (shRNA) against survivin gene into human T lymphoblastic leukemia cell line Jurkat, and to explore the effects on apoptosis and proliferation of transfected cells.
  
  
  METHODS
  The survivin-shRNA expression vector were constructed and transfected into Jurkat cells. Expression of survivin mRNA and protein were assessed by RT-PCR and Western blot analysis respectively. Apoptosis index of transfected Jurkat cells was quantified by flow cytometry. The potential of cell proliferation was described by cell growth curves.
  
  
  RESULTS
  In survivin-shRNA transfected Jurkat cells, survivin mRNA levels were significantly reduced by 66.67% ( transient transfection) and 60.69% ( stable transfection) respectively, compared with that in control-shRNA treated group and PBS treated group (P < 0.05); and the levels of survivin protein were significantly reduced by 63.41% (transient transfection) and 60.18% (stable transfection), compared with that in the two control groups (P < 0.05). Apoptosis index was significantly increased during both transient and stable transfection, respectively [(22. 41 +/- 2.83)% and (20.73 +/- 2.56)% (P < 0.05)]. Survivin-shRNA also inhibited the proliferation of Jurkat cells.
  
  
  CONCLUSIONS
  Vector-based survivin-shRNA can effectively reduce the expression of survivin gene, induce apoptosis
authors.affiliation: Department of Pathology, the First Afiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Cong-min
authors.initials: CM
authors.lastname: Gu
authors.forename: You-kai
authors.initials: YK
authors.lastname: Zhu
authors.forename: Hong-yang
authors.initials: HY
authors.lastname: Wu
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Liao
authors.forename: Han-liang
authors.initials: HL
authors.lastname: Lin
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D019169:Jurkat Cells; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000494:pharmacology*; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000077022:Survivin