title: Endogenous 15-deoxy-Delta(12,14)-prostaglandin J(2) synthesized by adipocytes during maturation phase contributes to upregulation of fat storage.
abstract: 15-Deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) has been identified as a natural ligand for peroxisome proliferator-activated receptor (PPAR) gamma to promote adipogenesis. However, it remains elusive about the ability of PPARgamma-expressing adipocytes to produce PGJ(2) series and the role in the life cycle of adipocytes. Here, we developed an enzyme-linked immunosorbent assay specific for 15d-PGJ(2). The analysis using this method revealed the increase in the endogenous synthesis of immunoreactive 15d-PGJ(2) in cultured adipocytes during the maturation phase. Further studies using cyclooxygenase inhibitors clarified the contribution of endogeous 15d-PGJ(2) produced by mature adipocytes to upregulation of fat storage in an autocrine manner.
authors.affiliation: Department of Life Science and Biotechnology, Shimane University, Matsue, Shimane 690-8504, Japan.
authors.forename: Md Abdul
authors.initials: MA
authors.lastname: Mazid
authors.forename: Abu Asad
authors.initials: AA
authors.lastname: Chowdhury
authors.forename: Kohjiro
authors.initials: K
authors.lastname: Nagao
authors.forename: Kohji
authors.initials: K
authors.lastname: Nishimura
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Jisaka
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nagaya
authors.forename: Kazushige
authors.initials: K
authors.lastname: Yokota
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D007527:Isoenzymes / Q000235:genetics; D051379:Mice; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000096:biosynthesis; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: C097240:15-deoxy-delta(12,14)-prostaglandin J2; D015415:Biomarkers; D007527:Isoenzymes; D011451:Prostaglandin-Endoperoxide Synthases; D015230:Prostaglandin D2