title: The anaphase-promoting complex is required in both dividing and quiescent cells during zebrafish development.
abstract: The anaphase-promoting complex/cyclosome (APC/C) regulates multiple stages of the cell cycle, most prominently mitosis. We describe zebrafish with mutations in two APC/C subunits, Cdc16 and Cdc26, whose phenotypes reveal a multifaceted set of defects resulting from the gradual depletion of the APC/C. First, loss of the APC/C in dividing cells results in mitotic arrest, followed by apoptosis. This defect becomes detectable in different organs at different larval ages, because the subunits of the APC/C are maternally deposited, are unusually stable, and are depleted at uneven rates in different tissues. Second, loss of the APC/C in quiescent or differentiated cells results in improper re-entry into the cell cycle, again in an apparently tissue-specific manner. This study is the first demonstration of both functions of the APC/C in a vertebrate organism and also provides an illustration of the surprisingly complex effects that essential, maternally supplied factors can have on the growing animal over a period of 10 days or longer.
authors.affiliation: Programs in Genetics and Developmental Biology, University of California-San Francisco, 1550 4th Street, San Francisco, CA 94158-2722, USA.
authors.forename: Ann M
authors.initials: AM
authors.lastname: Wehman
authors.forename: Wendy
authors.initials: W
authors.lastname: Staub
authors.forename: Herwig
authors.initials: H
authors.lastname: Baier
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics; D002874:Chromosome Mapping; D019926:Cyclin B; D017931:DNA Primers; D007150:Immunohistochemistry; D008938:Mitosis / Q000502:physiology*; D008954:Models, Biological; D008969:Molecular Sequence Data; D012160:Retina / Q000196:embryology; D017422:Sequence Analysis, DNA; D043743:Ubiquitin-Protein Ligase Complexes / Q000235:genetics* / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology*
chemical_list: D018797:Cell Cycle Proteins; D019926:Cyclin B; D017931:DNA Primers; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome