Study of growth parameters and potentialities of differentiation of multipotent mesenchymal stromal cells from rat bone marrow in vitro.
The dynamics of growth and proliferative activity of the population of multipotent mesenchymal stromal cells from rat bone marrow was studied during 7 passages. The efficiency of colony formation, the morphology of multipotent mesenchymal stromal cells, and the possibility of spontaneous and induced differentiation were studied. The rat bone marrow fibroblast-like multipotent mesenchymal stromal cells are capable of clonal growth; their proliferative activity and the yield remained high until passage 4, but then decreased. Induction of osteo- or adipogenic differentiation of bone marrow multipotent mesenchymal stromal cells increased the percentage of morphologically modified cells carrying specific markers.
Sergeeva
N S
NS
Department of Prediction of Conservative Treatment Efficiency, P. A. Hertzen Oncological Institute, Russian Ministry of Health.
Sviridova
I K
IK
Kirsanova
V A
VA
Akhmedova
S A
SA
Marshutina
N V
NV
Sergeeva
V S
VS
Bednik
D Yu
DY
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000378:metabolism