A role for Phospholipase D in Drosophila embryonic cellularization.
BACKGROUND
Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically-translocated Golgi-derived vesicles.


RESULTS
We describe here involvement of the signaling enzyme Phospholipase D (Pld) in regulation of this developmental step. Functional analysis using gene targeting revealed that cellularization is hindered by the loss of Pld, resulting frequently in early embryonic developmental arrest. Mechanistically, chronic Pld deficiency causes abnormal Golgi structure and secretory vesicle trafficking.


CONCLUSIONS
Our results suggest that Pld functions to promote trafficking of Golgi-derived fusion-competent vesicles during cellularization.
LaLonde
Mary
M
Department of Pharmacology and Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794-5140, USA. mary.lalonde@uhmc.sunysb.edu <mary.lalonde@uhmc.sunysb.edu>
Janssens
Hilde
H
Yun
Suyong
S
Crosby
Juan
J
Redina
Olga
O
Olive
Virginie
V
Altshuller
Yelena M
YM
Choi
Seok-Yong
SY
Du
Guangwei
G
Gergen
J Peter
JP
Frohman
Michael A
MA
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C / Q000032:analysis; D002522:Chlorocebus aethiops; D048749:Cytokinesis; D022162:Cytoplasmic Vesicles / Q000737:chemistry / Q000201:enzymology / Q000502:physiology; D004331:Drosophila melanogaster / Q000166:cytology* / Q000196:embryology* / Q000235:genetics / Q000502:physiology; D004789:Enzyme Activation; D005260:Female; D006056:Golgi Apparatus / Q000502:physiology; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D008566:Membranes / Q000378:metabolism; D009154:Mutation; D010739:Phospholipase D / Q000235:genetics / Q000502:physiology*; D023121:RNA, Messenger, Stored / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection
C515538:CX3CL1 protein, human; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C; D008565:Membrane Proteins; D023121:RNA, Messenger, Stored; D010739:Phospholipase D