DNA-catalyzed transfer of a reporter group.
Herein we describe a novel DNA-catalyzed transfer of a reporter group from a donating to an accepting oligo-peptide nucleic acid (PNA), generating high catalytic turnover numbers in a DNA-sequence specific manner. The demonstrated transfer of the Dabcyl group was designed to switch on emission of a fluorescein dye while switching off emission of a rhodamine dye. This setup allows the highly sensitive and selective detection of DNA-sequences in real time.
Grossmann
Tom N
TN
Institut für Chemie, Humboldt-Universität zu Berlin, Brook-Taylor-Strasse 2, D-12489 Berlin, Germany.
Seitz
Oliver
O
D001483:Base Sequence; D001693:Biological Transport, Active / Q000502:physiology; D015374:Biosensing Techniques; D002384:Catalysis; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D017930:Genes, Reporter; D009693:Nucleic Acid Hybridization; D012235:Rhodamines / Q000737:chemistry*; D013698:Templates, Genetic
C114557:5-carboxytetramethylrhodamine succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D012235:Rhodamines; C024098:6-carboxyfluorescein; D004247:DNA