title: [Investigation on rodents' natural infection of Orientia tsutsugamushi in some areas of Inner Mongolia and Xinjiang, China].
abstract: OBJECTIVE
  To investigate rodents' natural infection of Orientia tsutsugamushi (Ot) in some areas of Inner Mongolia and Xinjiang, China.
  
  
  METHODS
  DNAs were extracted from spleens of the captured mice and nested-polymerase chain reaction (nPCR) technique was used to detect the Ot-Sta56 gene. Six positive samples were sequenced and analyzed by Clustal X (5.0) and DNA Club software.
  
  
  RESULTS
  A total of 90 rodents were captured in Inner Mongolia, and the overall prevalence of Ot was 6.67%. There was no significant difference in infection rates among the positive rodents species. 20 rodents were captured in Xinjiang, and the prevalence of Ot was 5.00%. The geographical difference in infection rates was not statistically significant between Inner Mongolia and Xinjiang. 9 rodents were captured in farmlands of Inner Mongolia and Xinjiang but there was no positive samples found. 101 rodents were captured in grasslands, and the prevalence of Ot was 6.93%. The Sta56 gene nucleotide sequence homology to Karp strain of N59 (from Microtus maximowiczii), N69 (from Cricetulus barabensis) and X33(from Cricetus cricetus) was 99%. The sequence homology to Taitung-2 strain and TW461 strain of N65 (from C. barabensis) was 94%, and the sequence homology to Taitung-2 strain and TW461 strain of N88(from Apodemus agrarius) was also 94%. The sequence homology to Oishi strain of N90 (from A. agrarius) was 96.00%.
  
  
  CONCLUSIONS
  Our findings indicated that infections of Ot did exist in rodents captured from Inner Mongolia and Xinjiang. The genotypes of Ot in Inner Mongolia and Xinjiang were quite complex, with some of them belonged to Karp type, and the others belonged to Taitung-2, TW461 and Oishi types which providing evidence for further investigation on the scrub typhus fuci in the two areas.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yun-xi
authors.initials: YX
authors.lastname: Liu
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Wu
authors.forename: Qiu-min
authors.initials: QM
authors.lastname: Zhao
authors.forename: Pan-he
authors.initials: PH
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Wu-chun
authors.initials: WC
authors.lastname: Cao
mesh_terms: D000818:Animals; D002681:China; D005843:Geography; D012285:Orientia tsutsugamushi / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012377:Rodentia / Q000382:microbiology*; D012612:Scrub Typhus