title: Immobilization of enamel matrix derivate protein onto polypeptide multilayers. Comparative in situ measurements using ellipsometry, quartz crystal microbalance with dissipation, and dual-polarization interferometry.
abstract: The buildup of biodegradable poly(L-glutamic acid) (PGA) and poly(L-lysine) (PLL) multilayers on silica and titanium surfaces and the immobilization of enamel matrix derivate (EMD) protein was followed by utilizing in situ ellipsometry, quartz crystal microbalance with dissipation, and dual-polarization interferometry (DPI). The use of the relatively new DPI technique validated earlier published ellipsometry measurements of the PLL-PGA polypeptide films. The hydrophobic aggregating EMD protein was successfully immobilized both on top of and within the multilayer structures at pH 5.0. DPI measurements further indicated that the immobilization of EMD is influenced by the flow pattern during adsorption. The formed polypeptide-EMD multilayer films are of interest since it is known that EMD is able to trigger cell response and induce biomineralization. The multilayer films thus have potential to be useful as bioactive and biodegradable coatings for future dental implants.
authors.affiliation: YKI, Institute for Surface Chemistry, Box 5607, SE-114 86 Stockholm, Sweden.
authors.forename: Tobias J
authors.initials: TJ
authors.lastname: Halthur
authors.forename: Per M
authors.initials: PM
authors.lastname: Claesson
authors.forename: Ulla M
authors.initials: UM
authors.lastname: Elofsson
mesh_terms: D000818:Animals; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry*; D003746:Dental Enamel Proteins / Q000737:chemistry*; D015921:Dental Implants; D011099:Polyglutamic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D011107:Polylysine / Q000096:biosynthesis / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D003746:Dental Enamel Proteins; D015921:Dental Implants; C109069:enamel matrix proteins; D011107:Polylysine; D011099:Polyglutamic Acid; D012822:Silicon Dioxide; D014025:Titanium