title: Identification of bacteria associated with spreading odontogenic infections by 16S rRNA gene sequencing.
abstract: OBJECTIVE
  To determine the bacterial species associated with spreading odontogenic infections (SOIs).
  
  
  METHODS
  Pus samples from 4 cases of SOI were analyzed by microbiological culture methods for the presence of bacteria, and by polymerase chain reaction (PCR) amplification, cloning, and sequencing of bacterial 16S rRNA genes.
  
  
  RESULTS
  Culture methods identified species from the genera Prevotella, Streptococcus, and Fusobacterium, as well as anaerobic streptococci. Molecular detection methods identified a far more diverse microflora. The predominant genus detected was Prevotella, representing 102 (50.2%) of 203 clones analyzed. Prevotella oris was the most abundant species identified, representing 45 (22.2%) of 203 clones analyzed. Twelve clones (5.9%) represented uncultivable species, namely Prevotella PUS9.180, an uncultured Peptostreptococcus species, and an uncultured bacterium belonging to the Bacteroidetes phylum.
  
  
  CONCLUSIONS
  Prevotella species may play an important role in SOIs, and further work to examine in more detail the pathogenicity determinants of these organisms and associated host responses is warranted.
authors.affiliation: Infection and Immunity Section, University of Glasgow Dental School, Glasgow, UK. m.riggio@dental.gla.ac.uk
authors.forename: Marcello P
authors.initials: MP
authors.lastname: Riggio
authors.forename: Hiba
authors.initials: H
authors.lastname: Aga
authors.forename: Colin A
authors.initials: CA
authors.lastname: Murray
authors.forename: Margaret S
authors.initials: MS
authors.lastname: Jackson
authors.forename: Alan
authors.initials: A
authors.lastname: Lennon
authors.forename: Nicholas
authors.initials: N
authors.lastname: Hammersley
authors.forename: Jeremy
authors.initials: J
authors.lastname: Bagg
mesh_terms: D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005491:Focal Infection, Dental / Q000382:microbiology*; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000472:pathogenicity; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D017861:Porphyromonas / Q000472:pathogenicity; D018720:Prevotella / Q000472:pathogenicity*; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017422:Sequence Analysis, DNA; D013492:Suppuration / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S