Retinoic acid regulation of eye and testis-specific transcripts within a complex locus.
We previously used a yeast-based enhancer trap to identify a strong, retinoic acid response element (RARE). We have now characterized testis and eye transcripts that are adjacent to this regulatory element. Bioinformatics analysis of expressed sequence tag (EST) clones and RNase protection, reverse transcription-PCR, and Northern blot assays indicate that these two RNAs are transcribed from the same locus on opposite template strands. This positions the RARE upstream of the testis transcript and downstream of the eye transcript. Additionally, these two RNAs are embedded within the third intron of the 329kbp gene that encodes the Zinc Finger and BTB domain containing 7C protein (Zbtb7C). We present evidence indicating that the testis transcript is expressed primarily in spermatocytes and/or early round spermatids. Furthermore, our analyses of transcript levels in eyes and testes isolated from vitamin A deficient mice or from mice with defects in retinoid storage or signaling indicate that retinoids are required for expression in vivo.
Das
Pragnya
P
Department of Biochemistry and Molecular Biology (MSB E627), UMDNJ-New Jersey Medical School, 185 South Orange Avenue, P.O. Box 1709, Newark NJ 07101-1709, USA.
Doyle
Timothy J
TJ
Liu
Donglin
D
Kochar
Jaspreet
J
Kim
Kwan Hee
KH
Rogers
Melissa B
MB