[Optimizing process of four-effect countercurrent extraction of danshensu from Salvia miltiorrhiza].
OBJECTIVE
To investigate the process factors and characteristic of four-effect countercurrent extracting process of Danshensu from Salvia miltiorrhiza. Optimizing the parameters by using regression analysis and principal components analysis (PCA).


METHODS
Applying the orthogonal design method to optimize the extracting process. The factors of extraction time, solvent volume, extraction temperature and the degree of grinding were considered. And also, the new process was compared with the refluence process.


RESULTS
The optimal condition of four-effect countercurrent extraction of Danshensu from S. miltiorrhiza was 1 h extraction time, solvent to sample ratio of 12 mL x g(-1), 80 degrees C , 20 - 40 mesh of particle size. Compared with the refluence process, the countercurrent extracting process has the advantages of higher recovery, less solvent consumption and shorter extraction time.


CONCLUSIONS
The four-effect countercurrent extraction process has great advantages and is good for Chinese medicinal materials.
Cai
Ming
M
Department of Chinese Medicine Sciences and Engineering, Zhejiang University, Hangzhou 310027, China.
Chen
Yong
Y
Liu
Xue-Song
XS
Qu
Hai-Bin
HB
Cheng
Yi-Yu
YY
D007773:Lactates / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D025341:Principal Component Analysis; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*
D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid