Dimorphic gene expression patterns of anorexigenic and orexigenic peptides in hypothalamus account male and female hyperphagia in Akita type 1 diabetic mice.
Progression of the diabetic trait is usually more rapid in males than females. The major aim of the present study is to explore sexual dimorphism in the hypothalamus in Akita mice. Akita male mice develop hyperphagia, reducing anorexigenic proopiomelanocortin (POMC), and increasing orexigenic neuropeptide Y (NPY) mRNA levels compared with wt males. Serum leptin level was suppressed in Akita males, though castration improved these levels leading to reductions of hyperphagia and blood glucose levels. While Akita female mice also developed hyperphagia, there was no difference in POMC, NPY and leptin levels between Akita and wt females. Anorexigenic Cocaine- and amphetamine-regulated transcript (CART) and corticotrophin-releasing factor (CRF) mRNA levels in Akita females were decreased against wt females. Gonadectomy did not have any effect on the regulation of these neuropeptides. These data suggested that there is sexual dimorphism of neuronal regulation in hyperphagia in Akita mice.
Toyoshima
Megumi
M
Department of Health and Environmental Sciences, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan.
Asakawa
Akihiro
A
Fujimiya
Mineko
M
Inoue
Kayoko
K
Inoue
Sumiko
S
Kinboshi
Masato
M
Koizumi
Akio
A
D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D004435:Eating; D005260:Female; D015870:Gene Expression; D007031:Hypothalamus / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009479:Neuropeptides / Q000378:metabolism*; D000068797:Orexins; D012727:Sex Characteristics; D012737:Sex Factors
D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins