title: Chemistry-based RNA technologies: demonstration of usefulness of libraries of ribozymes and short hairpin RNAs (shRNAs).
abstract: Mechanism of action of hammerhead ribozymes has been investigated and their intracellular activities have been improved. Based on the improved ribozymes and more recently discovered natural RNAi, we have created libraries of both ribozymes and short hairpin RNAs (shRNAs). The introduction of a library of active ribozymes or shRNAs into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function.
authors.affiliation: Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-8656, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyagishi
authors.forename: Sahohime
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Hideo
authors.initials: H
authors.lastname: Akashi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Taro
authors.initials: T
authors.lastname: Fukao
authors.forename: Yoko
authors.initials: Y
authors.lastname: Fukuda
authors.forename: Masayuki
authors.initials: M
authors.lastname: Sano
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kato
authors.forename: Yasuomi
authors.initials: Y
authors.lastname: Takagi
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Masaki
authors.initials: M
authors.lastname: Warashina
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kuwabara
authors.forename: Shinya Y
authors.initials: SY
authors.lastname: Sawata
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Shun-ichi
authors.initials: S
authors.lastname: Kawahara
authors.forename: Kaul C
authors.initials: KC
authors.lastname: Sunil
authors.forename: Renu
authors.initials: R
authors.lastname: Wadhwa
authors.forename: Kazunari
authors.initials: K
authors.lastname: Taira
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D020650:Combinatorial Chemistry Techniques; D006801:Humans; D051379:Mice; D010641:Phenotype; D034622:RNA Interference; D016337:RNA, Catalytic / Q000737:chemistry*; D022661:RNA, Untranslated / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016337:RNA, Catalytic; D022661:RNA, Untranslated; C427204:hammerhead ribozyme