title: Morphological signs of apoptosis in axotomized ganglion cells of the rabbit retina.
abstract: Optic nerve section in mammals induces apoptotic death of retinal ganglion cells (RGCs). However, a small population of RGCs survives for a relatively long time. These cells experience significant morphological changes due to the apoptotic process, but some of these changes are not clearly differentiated from those experienced in necrotic cells. In the present work, rabbit RGCs were studied 1 month after optic nerve section using light microscopy after neurobiotin injection, transmission electron microscopy (EM) and scanning electron microscopy (SEM). Apoptosis was identified by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling and characteristic signs of apoptosis were observed in the EM images. Ultrastructural analyses showed vacuolar degeneration in the cytoplasm and normal cellular structure loss. Signs of membrane changes were observed in axotomized RGCs by SEM. Early changes seen in the cell membrane suggest that axotomy may cause important changes in the cytoskeleton. We conclude that characteristic signs of apoptosis at the cell membrane level are clearly observed in rabbit RGCs after axotomy and they may be responsible for the cellular death.
authors.affiliation: Department of Physiology, School of Medicine, University of Alcalá, Alcalá de Henares, 28871 Madrid, Spain. francisco.germain@uah.es
authors.forename: F
authors.initials: F
authors.lastname: Germain
authors.forename: E
authors.initials: E
authors.lastname: Fernández
authors.forename: P
authors.initials: P
authors.lastname: de la Villa
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D019771:Axotomy; D001710:Biotin / Q000031:analogs & derivatives; D002462:Cell Membrane / Q000473:pathology; D002470:Cell Survival / Q000502:physiology; D003593:Cytoplasm / Q000473:pathology; D003599:Cytoskeleton / Q000473:pathology; D003712:Dendrites / Q000473:pathology; D020287:In Situ Nick-End Labeling; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009410:Nerve Degeneration / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology*; D020221:Optic Nerve Injuries / Q000473:pathology* / Q000503:physiopathology*; D011817:Rabbits; D012165:Retinal Ganglion Cells / Q000473:pathology*
chemical_list: C070614:neurobiotin; D001710:Biotin