title: Functional analysis of factors involved in trans-translation.
abstract: trans-translation is a rescue system for stalled ribosomes to terminate the translation for recycling of ribosome and lead the incomplete protein on the stalled ribosome to degradation. In trans-translation, several proteins and RNAs are involved. tmRNA and SmpB are only factors known to be essential for trans-translation. EF-Tu and EF-G functions in translation and also are seemed to function in trans-translation. Ribosomal protein S1 is known to bind tmRNA. In this study, we cloned the gene of tmRNA from Thermus thermophilus and analyzed the functions of factors involved in trans-translation in vitro.
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, 036-8561, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Takada
authors.forename: Chie
authors.initials: C
authors.lastname: Takemoto
authors.forename: Masahito
authors.initials: M
authors.lastname: Kawazoe
authors.forename: Takayuki
authors.initials: T
authors.lastname: Konno
authors.forename: Noriko
authors.initials: N
authors.lastname: Matsuta
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kurita
authors.forename: Krisana
authors.initials: K
authors.lastname: Asano
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Akira
authors.initials: A
authors.lastname: Muto
authors.forename: Hyouta
authors.initials: H
authors.lastname: Himeno
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010303:Paromomycin / Q000494:pharmacology; D010443:Peptide Chain Termination, Translational; D012329:RNA, Bacterial / Q000737:chemistry* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D016963:Thermus thermophilus / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; D012269:Ribosomal Proteins; C096222:tmRNA; D010303:Paromomycin