title: Tomographic molecular imaging and 3D quantification within adult mouse organs.
abstract: A convenient technology to quantify three-dimensional (3D) morphological features would have widespread applications in biomedical research. Based on combined improvements in sample preparation, tomographic imaging and computational processing, we present a procedure for high-resolution 3D quantification of structures within intact adult mouse organs. Using the nonobese diabetic (NOD) mouse model, we demonstrate a correlation between total islet beta-cell volume and the onset of type-1 diabetes.
authors.affiliation: Umeå Center for Molecular Medicine, Umeå University, S-901 87, Umeå, Sweden.
authors.forename: Tomas
authors.initials: T
authors.lastname: Alanentalo
authors.forename: Amir
authors.initials: A
authors.lastname: Asayesh
authors.forename: Harris
authors.initials: H
authors.lastname: Morrison
authors.forename: Christina E
authors.initials: CE
authors.lastname: Lorén
authors.forename: Dan
authors.initials: D
authors.lastname: Holmberg
authors.forename: James
authors.initials: J
authors.lastname: Sharpe
authors.forename: Ulf
authors.initials: U
authors.lastname: Ahlgren
mesh_terms: D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000175:diagnosis* / Q000503:physiopathology; D004195:Disease Models, Animal; D018450:Disease Progression; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007515:Islets of Langerhans / Q000473:pathology*; D051379:Mice; D010179:Pancreas / Q000473:pathology*; D012680:Sensitivity and Specificity; D014054:Tomography / Q000379:methods*