Beneficial effects of autologous bone marrow mononuclear cell transplantation against elastase-induced emphysema in rabbits.
The authors investigated whether autologous bone marrow mononuclear cell (BMC) transplantation via the left and right main bronchi would mitigate elastase-induced pulmonary emphysema in rabbits. Four weeks after elastase administration, rabbits also receiving BMCs showed significantly better pulmonary function (FVC, FEV100, FEVPEF) and smaller alveolar airspaces, as indicated by a smaller mean linear intercept, than those receiving porcine pancreatic elastase (PPE) (200 U/kg) alone via the left and right main bronchi. BMCs also significantly reduced cell counts in bronchoalveolar lavage fluid, the incidence of apoptotic (TUNEL-positive) cells and matrix metalloproteinase (MMP)-2 expression, while increasing numbers of proliferative (Ki-67-positive) cells. Thus, BMCs may inhibit the progression to emphysema by attenuating inflammation, MMP-2 expression, and apoptosis, while enhancing alveolar cell proliferation.
Yuhgetsu
Hideyuki
H
Second Department of Internal Medicine, Regeneration and Advanced Medical Science, Graduate School of Medicine, Gifu University, Gifu, Japan.
Ohno
Yasushi
Y
Funaguchi
Norihiko
N
Asai
Toshihiro
T
Sawada
Masahiro
M
Takemura
Genzou
G
Minatoguchi
Shinya
S
Fujiwara
Hisayoshi
H
Fujiwara
Takako
T
D000818:Animals; D017209:Apoptosis; D016026:Bone Marrow Transplantation; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D004195:Disease Models, Animal; D004646:Emphysema / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D019084:Fluorescent Antibody Technique, Indirect; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009000:Monocytes / Q000637:transplantation*; D010196:Pancreatic Elastase / Q000009:adverse effects; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011817:Rabbits; D012129:Respiratory Function Tests; D014182:Transplantation, Autologous
D019394:Ki-67 Antigen; D010196:Pancreatic Elastase; D020778:Matrix Metalloproteinase 2