A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors.
A 66164-bp cryptic plasmid, pEIB1, was isolated from strain Vibrio anguillarum MVM425 and sequenced. A plasmid carrying a 1089-bp fragment, containing the minimal replication region of pEIB1, a kanamycin-resistance marker and an L-arabinose promoter, designated pEC, was maintained as a high copy plasmid in E. coli and stably inherited in the absence of antibiotic selection. Significantly, pEC was compatible with the widely used ColE1, pSC101 and p15A replicons making it a useful tool for a dual-plasmid expression system.
Zhang
Huijing
H
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Wu
Haizhen
H
Zhang
Huizhan
H
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D018628:Gene Dosage / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods; D014733:Vibrio / Q000235:genetics*