Structural property and enzymatic response of oxanine in DNA strands.
Oxanine (Oxa, O), one of the major products generated from guanine (Gua) by nitrosative oxidation has been expected as mutagenic lesion involved in NO- or HNO(2) -induced genotoxicity. Here, to elucidate the biological meaning of Oxa in DNA strands, several kinds of Oxa-containing oligodeoxynucleotides (Oxa-ODNs) were synthesized and applied to biophysical and biochemical characterization. CD and NMR analyses revealed that the conformations of all the Oxa-containing duplexes are basically B-type without causing any severe distortion in the whole DNA structure. It was also determined that restriction endonucleases recognize and cleave the specific base-sequence even when Gua was substituted by Oxa in the sequence. When Oxa-ODN was testified as substrates for other DNA-relevant enzymes, the enzymatic functions were not largely affected by Oxa.
Pack
Seung Pil
SP
Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan.
Doi
Akihiro
A
Nonogawa
Mitsuru
M
Kamisetty
Nagendra Kumar
NK
Devarayapalli
Kamakshaiah Charyulu
KC
Kodaki
Tsutomu
T
Makino
Keisuke
K
D000469:Alkaline Phosphatase / Q000378:metabolism; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism; D011684:Purine Nucleosides / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D011684:Purine Nucleosides; C468766:oxanine; D004247:DNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D004262:DNA Restriction Enzymes; D000469:Alkaline Phosphatase