title: [Preparation and identification of anti-human epididymal sperm protein P34H monoclonal antibody].
abstract: OBJECTIVE
  To prepare and identify anti-human epididymal sperm protein P34H monoclonal antibody (McAb).
  
  
  METHODS
  The previously purified recombinant P34H protein was used as an antigen to immunize BALB/c mouse. Cell strains secreting anti-P34H McAb were established by hybridoma technique and then ascitic fluid-type McAb prepared. The sensitivity and specificity of McAb were detected by ELISA and Western blot, respectively.
  
  
  RESULTS
  One strain (2C4) of IgG1 Kappa anti-P34H McAb was harvested. The titer detected by ELISA technique was 1:10(3) - 1:10(5). Western blot of healthy human sperm samples and purified recombinant P34H antigen probed with the prepared McAb were immunoreactive at 34,000 and 27,000, respectively.
  
  
  CONCLUSIONS
  Anti-P34H McAb has been prepared successfully by the above methods, which may provide a powerful tool for exploring the relationship between P34H and male fertility.
authors.affiliation: Laboratory of Reproduction & Genetics, School of Clinical Medicine, Nanjing University Medical College, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China. xiaxy@androl.cn
authors.forename: Xin-yi
authors.initials: XY
authors.lastname: Xia
authors.forename: Yong-ming
authors.initials: YM
authors.lastname: Wu
authors.forename: Lian-jun
authors.initials: LJ
authors.lastname: Pan
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.forename: Bao-fang
authors.initials: BF
authors.lastname: Jin
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Huang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D013401:Sugar Alcohol Dehydrogenases; C023300:L-xylulose reductase