FSH receptor genotype is associated with pregnancy but not with ovarian response in IVF.
Two very common single nucleotide polymorphisms at positions 307 and 680 in exon 10 of the FSH receptor gene have been associated with ovarian response in IVF. This observational study evaluated the role of the FSH receptor genotype in the prediction of poor response and clinical pregnancy in IVF in comparison with other markers, such as age, basal FSH, anti-Müllerian hormone and antral follicle count. In addition, the in-vitro cAMP response towards recombinant FSH in cultured granulosa cells of patients with different FSH receptor genotypes was determined. A total of 105 IVF patients undergoing ovarian stimulation in a long suppression protocol were included in the study. The ovarian response was comparable between patients with different FSH receptor genotypes. Patients with polymorphism Ser/Ser had implantation and pregnancy rates that were three times higher compared with patients with polymorphism Asn/Asn. FSH receptor genotype was not associated with a poor response in IVF, but showed a positive association with pregnancy, independent of age. There was no difference in cAMP production in cultured granulosa cells of patients with different FSH receptor genotypes (n=62). It is concluded that FSH receptor genotype is associated with pregnancy in IVF, but not with ovarian response.
Klinkert
Ellen R
ER
Department of Reproductive Medicine, Division of Perinatology and Gynecology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands. erklinkert@planet.nl
te Velde
Egbert R
ER
Weima
Sjerp
S
van Zandvoort
Peter M
PM
Hanssen
Rob G J M
RG
Nilsson
Philomeen R
PR
de Jong
Frank H
FH
Looman
Caspar W N
CW
Broekmans
Frank J M
FJ
D000328:Adult; D000242:Cyclic AMP / Q000378:metabolism; D010064:Embryo Implantation; D005260:Female; D005307:Fertilization in Vitro; D005838:Genotype; D006801:Humans; D007246:Infertility; D016017:Odds Ratio; D010053:Ovary / Q000187:drug effects / Q000502:physiology; D010062:Ovulation Induction; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011256:Pregnancy Outcome; D011446:Prospective Studies; D011962:Receptors, FSH / Q000235:genetics*; D016896:Treatment Outcome
D011962:Receptors, FSH; D000242:Cyclic AMP