Ocular drug delivery by liposome-chitosan nanoparticle complexes (LCS-NP).
This study evaluated in vitro and in vivo a colloidal nanosystem with the potential to deliver drugs to the ocular surface. This nanosystem, liposome-chitosan nanoparticle complexes (LCS-NP), was created as a complex between liposomes and chitosan nanoparticles (CS-NP). The conjunctival epithelial cell line IOBA-NHC was exposed to several concentrations of three different LCS-NP complex to determine the cytotoxicity. The uptake of LCS-NP by the IOBA-NHC conjunctival cell line and by primary cultured conjunctival epithelial cells was examined by confocal microscopy. Eyeball and lid tissues from LCS-NP-treated rabbits were evaluated for the in vivo uptake and acute tolerance of the nanosystems. The in vitro toxicity of LCS-NP in the IOBA-NHC cells was very low. LCS-NPs were identified inside IOBA-NHC cells after 15 min and inside primary cultures of conjunctival epithelial cells after 30 min. Distribution within the cells had different patterns depending on the LCS-NP formulation. Fluorescence microscopy of the conjunctiva revealed strong cellular uptake of LCS-NP in vivo and less intensive uptake by the corneal epithelium. No alteration was macroscopically observed in vivo after ocular surface exposure to LCS-NP. Taken together, these data demonstrate that LCS-NPs are potentially useful as drug carriers for the ocular surface.
Diebold
Yolanda
Y
IOBA, University of Valladolid, Ramón y Cajal 7, E-47005 Valladolid, Spain. yol@ioba.med.uva.es
Jarrín
Miguel
M
Sáez
Victoria
V
Carvalho
Edison L S
EL
Orea
María
M
Calonge
Margarita
M
Seijo
Begoña
B
Alonso
María J
MJ
D001672:Biocompatible Materials; D002461:Cell Line, Transformed; D048271:Chitosan; D016503:Drug Delivery Systems; D005123:Eye; D006801:Humans; D008081:Liposomes; D053758:Nanoparticles
D001672:Biocompatible Materials; D008081:Liposomes; D048271:Chitosan