Spectroscopic analysis of DNA duplexes including T-T mismatches.
The DNA duplex with tandem T x T mismatches has been studied by NMR spectroscopy. For this study, we synthesized decamer duplex with two successive T x T mismatches, TT10:d(CGCGTTGTCC) x d(GGACTTCGCG). From two-dimensional (2D) 1H-1H NOESY spectrum, we were able to trace sequential NOE walks between base protons and anomeric protons (H1'), and assigned all the base protons and anomeric protons. These results indicate that the overall structure of the duplex is a right-handed duplex, with two successive T x T mismatches stacked in the helix.
Yamaguchi
Hiroshi
H
Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan.
Oda
Shuji
S
Kondo
Yoshinori
Y
Ono
Akira
A
Tanaka
Yoshiyuki
Y
D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis* / Q000235:genetics; D011740:Pyrimidine Dimers / Q000032:analysis* / Q000235:genetics
D009692:Nucleic Acid Heteroduplexes; D011740:Pyrimidine Dimers; D004247:DNA