Multi-nanopore force spectroscopy for DNA analysis.
The need for low-cost DNA sequence detection in clinical applications is driving development of new technologies. We demonstrate a method for detection of mutations in a DNA sequence purely by electronic means, and without need for fluorescent labeling. Our method uses an array of nanopores to perform synchronized single-molecule force spectroscopy measurements over many molecules in parallel, yielding detailed information on the kinetics of hundreds of molecule dissociations in a single measurement.
Tropini
Carolina
C
Department of Physics and Astronomy, University of British Columbia, Vancouver, BC, Canada.
Marziali
Andre
A
D009154:Mutation; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D009841:Oligonucleotides / Q000032:analysis*; D011061:Poly A / Q000032:analysis*; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods
D009841:Oligonucleotides; D011061:Poly A