Investigation of ferrocenyl carbodiimide (FCDI) in the modification reaction of nucleic acids.
Ferrocenyl carbodiimide (FCDI) was allowed to react with thymine or guanine bases of single stranded DNA in borate buffer (pH 9.5) at 37 degrees C for a short period of time. The reactivity of FCDI with the guanine bases was smaller than that with the thymine ones and the prolongation of the reaction time resulted in a lower yield. The reactivity for double stranded oligonucleotide was extremely decreased from that for the single stranded one. One base mismatched double stranded oligonucleotides could react with FCDI, albeit slowly. These results suggested that the reactivity of FCDI was variable depending on the kind of bases and the oligonucleotide structure.
Mukumoto
Kosuke
K
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
Nojima
Takahiko
T
Takenaka
Shigeori
S
D020137:Base Pair Mismatch; D002234:Carbodiimides / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D013941:Thymine / Q000737:chemistry
D002234:Carbodiimides; D004277:DNA, Single-Stranded; D005296:Ferrous Compounds; D009841:Oligonucleotides; C517870:ferrocenyl carbodiimide; D004247:DNA; D013941:Thymine