Effects of xapA and guaA disruption on inosine accumulation in Escherichia coli.
A xapA-disrupted mutant was studied to minimize hypoxanthine production and to improve inosine productivity in mutants of Escherichia coli. The xapA-disrupted mutant accumulated 5.6 g/l of inosine from 40 g/l of glucose, while the parent strain accumulated 4.6 g/l. This result indicates that xapA is activated in xapA-positive inosine-producers and that xapA disruption might be useful for improving inosine productivity.
Shimaoka
Megumi
M
Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.
Takenaka
Yasuhiro
Y
Mihara
Yoko
Y
Kurahashi
Osamu
O
Kawasaki
Hisashi
H
Matsui
Hiroshi
H
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D007288:Inosine / Q000378:metabolism*
D029968:Escherichia coli Proteins; D007288:Inosine