Biophysical and biochemical properties of oxanine-containing oligodeoxynucleotide.
Since oxanine (Oxa) was found as one of the major products from nitrosative oxidation of guanine, Oxa has been expected as mutagenic lesion related to NO-induced DNA damage. Here, to elucidate the mutagenic significance of Oxa in vitro, several Oxa-containing oligodeoxynucleotides (Oxa-ODNs) were synthesized and subjected to biophysical and biochemical characterization. Oxa-ODNs were analyzed in terms of their duplex stabilities and DNA polymerase responses. These results will be helpful in understanding the genotoxic pattern induced by Oxa.
Pack
Seung Pil
SP
International Innovation Center, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan.
Nonogawa
Mitsuru
M
Kamisetty
Nagendra Kumar
NK
Kodaki
Tsutomu
T
Makino
Keisuke
K
D004256:DNA Polymerase I / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011684:Purine Nucleosides / Q000737:chemistry*; D013696:Temperature
D009838:Oligodeoxyribonucleotides; D011684:Purine Nucleosides; C468766:oxanine; D004256:DNA Polymerase I