The protective effects of phenolic constituents from Gastrodia elata on the cytotoxicity induced by KCl and glutamate.
Seven phenolic compounds (1-7) were isolated from the tubers of Gastrodia elata. Their structures were elucidated on the basis of MS and NMR spectral data. p-Ethoxymethyl phenyl-O-beta-D-glucoside (1) was proved to be a new compound, with N-(p-hydroxybenzyl)-adenosine (7) isolated from this plant for the first time. In this study, the protective effects of the six constituents (1-6) on PC12 cells against the cytotoxicity induced by KCl and glutamate were also investigated. The viability of the PC12 cells was significantly enhanced by pretreatment with the six phenolic constituents.
Huang
Zhan-Bo
ZB
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Wu
Zhe
Z
Chen
Fa-Kui
FK
Zou
Li-Bo
LB
D000241:Adenosine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001593:Benzyl Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D005019:Ethyl Ethers / Q000302:isolation & purification / Q000494:pharmacology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D031668:Gastrodia / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D018698:Glutamic Acid / Q000633:toxicity*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology; D016716:PC12 Cells; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D035281:Plant Tubers / Q000737:chemistry; D011189:Potassium Chloride / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D051381:Rats; D013778:Tetrazolium Salts; D013844:Thiazoles
D000972:Antineoplastic Agents, Phytogenic; D001593:Benzyl Compounds; D005019:Ethyl Ethers; D018691:Excitatory Amino Acid Antagonists; D005960:Glucosides; C515642:N-(p-hydroxybenzyl)-adenosine; D018696:Neuroprotective Agents; D010636:Phenols; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; C515641:p-ethoxymethyl phenyl-O-beta-D-glucoside; D018698:Glutamic Acid; D011189:Potassium Chloride; C022616:thiazolyl blue; D000241:Adenosine