The molecular-thermometer based on B-Z transition of DNA.
A molecular thermometer utilizing the different pi-stacking of B- and Z-DNA was designed. Aminopurine, which is a fluorescent probe for charge-transfer processes, was introduced into B- and Z-DNA and the emission monitored. Z-DNA showed a marked increase in fluorescence compared with B-DNA, with the fluorescence intensity correlating linearly and reproducibly with temperature.
Tashiro
Ryu
R
Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Surugadai, Kanda, Chiyoda, Tokyo 101-0062, Japan.
Sugiyama
Hiroshi
H
D015075:2-Aminopurine / Q000737:chemistry; D043542:DNA, Z-Form / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D013821:Thermometers
D043542:DNA, Z-Form; D015075:2-Aminopurine