Ribozyme technology for cancer gene target identification and validation.
Ribozymes are naturally occurring RNAs with catalytic activities including cis- or trans- cleavage of RNA at predefined sequence sites. This activity has been exploited for specific gene inactivation in cells during the last two decades, and ribozymes have been important functional genomics tools, especially in the pre-RNAi era. It has also been broadly applied in drug target identification and validation in pharmaceutical R&D. This chapter covers many application principles and case studies of ribozyme technology in the areas of cancer research. We also described RNAi applications in some of the same studies for comparison. Although RNAi may be more effective than ribozymes in many respects, they are nonetheless built on many of the same principles.
Li
Qi-Xiang
QX
Immusol, Inc., San Diego, California 92121, USA.
Tan
Philip
P
Ke
Ning
N
Wong-Staal
Flossie
F
D000818:Animals; D015195:Drug Design; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D023281:Genomics / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D016337:RNA, Catalytic; D034741:RNA, Small Interfering
D016337:RNA, Catalytic; D034741:RNA, Small Interfering