Expression and localization of the progesterone receptor in mouse and human reproductive organs.
The effects of gonadotropins on progesterone receptor (PR) expression and localization in the mouse oviduct, uterus, and ovary was examined. In the oviduct ciliated epithelial cells of adult mice and human revealed a unique PR localization to the lower half of the motile cilia whereas the nuclei were unstained or faintly stained. Pubertal female mice were further studied by confocal laser scanning microscopy and western blotting before and after injection with FSH and LH followed by human chorionic gonadotropin (hCG) injection after a 48-h period. PR immunolocalization to the oviduct cilia was greatly increased in pubertal mice upon hCG stimulation. In neighboring goblet cells, the PR staining was confined to the nuclei. Nuclear PR localization was evident in epithelial cells of the uterus as well as in a fraction of stromal and muscle cells. Staining intensity and number of stained cells was not affected by hormone stimulation. In the ovary, weak PR immunolocalization was observed in unprimed animals but increased significantly after hCG stimulation. In granulosa cells of preovulatory follicles PR was exclusively observed in mural cells, whereas cumulus cells remained negative. At all stages examined, primary granulosa cell cilia lacked PR staining. SDS-PAGE and western blotting analysis of tissues from oviduct, uterus, and ovary confirmed antibody specificity, and identified two bands corresponding to the PR isoforms PR-A and PR-B. Upon hCG stimulation, a new band cross-reacting with anti-PR emerged above the PR-A form in oviduct fractions, suggesting LH-induced phosphorylation of PR-A. We suggest that ciliary PR in the oviduct plays a role in progesterone signaling after ovulation, possibly via non-genomic events. These novel findings warrant further studies of oviduct and postovulatory signaling events and suggest a sensory role for oviduct cilia in the process of oocyte transport/fertilization.
Teilmann
Stefan Cuoni
SC
Laboratory of Reproductive Biology, Juliane Marie Center, Rigshospitalet, Copenhagen, Denmark.
Clement
Christian Alexandro
CA
Thorup
Jørgen
J
Byskov
Anne Grete
AG
Christensen
Søren Tvorup
ST
D000328:Adult; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002467:Cell Nucleus / Q000737:chemistry; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003593:Cytoplasm / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000737:chemistry; D005187:Fallopian Tubes / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D005836:Genitalia, Female / Q000737:chemistry*; D006107:Granulosa Cells / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D010053:Ovary / Q000737:chemistry; D011980:Receptors, Progesterone / Q000032:analysis* / Q000235:genetics; D014599:Uterus / Q000737:chemistry
D006063:Chorionic Gonadotropin; D011980:Receptors, Progesterone