Connective tissue growth factor (CTGF) expression and outcome in adult patients with acute lymphoblastic leukemia.
We compared the gene expression profile of adult acute lymphoblastic leukemia (ALL) to normal hematopoietic and non-ALL samples using oligonucleotide arrays. Connective tissue growth factor (CTGF) was the highest overexpressed gene in B-cell ALL compared with the other groups, and displayed heterogeneous expression, suggesting it might have prognostic relevance. CTGF expression was examined by quantitative reverse transcriptase-polymerase chain reaction (ORT-PCR) on 79 adult ALL specimens. CTGF expression levels were significantly increased in ALL cases with B-lineage (P < .001), unfavorable cytogenetics (P < .001), and blasts expressing CD34 (P < .001). In a multivariate proportional hazards model, higher CTGF expression levels corresponded to worsening of overall survival (OS; hazard ratio 1.36, for each 10-fold increase in expression; P = .019). Further studies are ongoing to confirm the prognostic value of CTGF expression in ALL and to investigate its role in normal and abnormal lymphocyte biology.
Sala-Torra
Olga
O
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA. osala@fhcrc.org
Gundacker
Holly M
HM
Stirewalt
Derek L
DL
Ladne
Paula A
PA
Pogosova-Agadjanyan
Era L
EL
Slovak
Marilyn L
ML
Willman
Cheryl L
CL
Heimfeld
Shelly
S
Boldt
David H
DH
Radich
Jerald P
JP
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000235:genetics / Q000401:mortality; D016022:Case-Control Studies; D055513:Connective Tissue Growth Factor; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000401:mortality; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate
C526317:CCN2 protein, human; D017931:DNA Primers; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D055513:Connective Tissue Growth Factor