Precisely controlled intracellular delivery of DNA-peptide conjugates.
Precisely controlled intracellular delivery of oligonucleotides was achieved by conjugation with signal peptides and amphiphilic designed peptides. Localization in the cellular nucleus was accelerated by nuclear localizing signals derived from naturally occurring viral proteins and arginine rich designed peptides, and cytoplasmic localization was enhanced by nuclear export signals and membrane fusion peptides.
Kubo
Takanori
T
Department of Biological and Environmental Chemistry, School of Science and Technology, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
Kanno
Ken-ichi
K
Rumiana
Bakalova
B
Ohba
Hideki
H
Fujii
Masayuki
M
D000595:Amino Acid Sequence; D016923:Cell Death / Q000187:drug effects; D004247:DNA / Q000138:chemical synthesis / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D016503:Drug Delivery Systems; D006801:Humans; D019169:Jurkat Cells; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D016914:Ribonuclease H / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism
D016376:Oligonucleotides, Antisense; D010455:Peptides; D004247:DNA; D019098:Telomerase; D003851:Deoxyribonucleases; D016914:Ribonuclease H