title: Sensitive cell viability assay for use in drug screens and for studying the mechanism of action of drugs in Dictyostelium discoideum.
abstract: In order to increase the effectiveness of Dictyostelium discoideum as a lead genetic model for drug discovery, a luminescence-based assay has been adapted and standardized for sensitive and rapid cell viability measurements. The applicability of the assay was demonstrated by measuring the cytotoxicity of several drugs in wild-type and mutant cells. The robustness and ease of the assay demonstrate that it can be used in high-throughput applications such as drug or mutant screens. Conclusions from these studies are applicable to evaluating cell viability assays in other systems as well.
authors.affiliation: University of Missouri, Columbia, MO 65211-7400, USA.
authors.forename: Junxia
authors.initials: J
authors.lastname: Min
authors.forename: Priya
authors.initials: P
authors.lastname: Sridevi
authors.forename: Stephen
authors.initials: S
authors.lastname: Alexander
authors.forename: Hannah
authors.initials: H
authors.lastname: Alexander
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology; D004023:Dictyostelium / Q000187:drug effects*; D012680:Sensitivity and Specificity
chemical_list: D002945:Cisplatin