title: Chloroplast SecA and Escherichia coli SecA have distinct lipid and signal peptide preferences.
abstract: Like prokaryotic Sec-dependent protein transport, chloroplasts utilize SecA. However, we observe distinctive requirements for the stimulation of chloroplast SecA ATPase activity; it is optimally stimulated in the presence of galactolipid and only a small fraction of anionic lipid and by Sec-dependent thylakoid signal peptides but not Escherichia coli signal peptides.
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269-3125, USA.
authors.forename: Changqi
authors.initials: C
authors.lastname: Sun
authors.forename: Sharyn L
authors.initials: SL
authors.lastname: Rusch
authors.forename: Jinoh
authors.initials: J
authors.lastname: Kim
authors.forename: Debra A
authors.initials: DA
authors.lastname: Kendall
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D060365:Chloroplast Proteins; D002942:Circular Dichroism; D031541:Fluorescence Resonance Energy Transfer; D038983:Galactolipids / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010715:Phosphatidylglycerols / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000235:genetics; D000069816:SEC Translocation Channels; D000081416:SecA Proteins
chemical_list: D001426:Bacterial Proteins; D060365:Chloroplast Proteins; D038983:Galactolipids; D008055:Lipids; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010715:Phosphatidylglycerols; D010940:Plant Proteins; D021382:Protein Sorting Signals; D000069816:SEC Translocation Channels; C007388:digalactosyldiacylglycerol; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins