title: Prevalence & significance of hepatitis C virus (HCV) seropositivity in blood donors.
abstract: OBJECTIVE
  The clinical significance of anti HCV antibodies in healthy blood donors remains uncertain. These donors are usually asymptomatic and it is difficult to elicit risk factors of acquiring HCV infection during pre-donation questioning. Limited information on donor recall and follow up studies on anti HCV positive blood donors have been reported from India. Paucity of data which is likely to have an impact on safe blood transfusion programme has prompted us to undertake this study to assess the significance of HCV seropositivity in blood donors with respect to their clinical, biochemical and virological profile.
  
  
  METHODS
  A total of 16,250 blood units were screened for the mandatory tests using third generation ELISA (anti HIV 1&2, anti HCV, HBsAg), VDRL and peripheral smear for malaria. Donors reactive for anti HCV were informed. Repeat anti HCV reactive donors were subjected to detailed clinical history focusing on risk factors for HCV transmission. The blood tests included liver function tests (LFT), coagulation and autoimmune profile, qualitative serum cryoglobulins and HCV RNA detection. These donors were followed at 2-3 monthly intervals for a minimum period of six months by LFT.
  
  
  RESULTS
  An overall seropositivity of 0.44 per cent (72/16,250) was observed in our donors which was significantly lower in first time, young voluntary donors as compared to replacement donors (0.27 vs. 0.60%). In contrast to drug abuse (6.4%) we found minor percutaneous routes like sharing of shaving kits or visit to a road side barber (32%) as the major risk factor for HCV transmission. There was no prior history of blood transfusion in any of these donors; however history of some surgical procedures was present in 25.8 per cent. Raised transaminases and HCV viraemia were observed in 87 and 71 per cent donors respectively. An association was observed between HCV RNA when the ELISA ratio was >5.
  
  
  CONCLUSIONS
  Voluntary donors form a safe source of blood supply and efforts should be made to increase this precious source to 100 per cent. Abbreviated behavioural donor screening questionnaire for repeat donors is not advisable. Awareness and education of donors is required regarding modes of HCV transmission. HCV positive donors should be informed about their disease, counselled and referred to hepatologist, and permanently deferred for future donations.
authors.affiliation: Departments of Transfusion Medicine, Postgraduate Institute of Medical Education & Research, Chandigarh, India.
authors.forename: Beenu
authors.initials: B
authors.lastname: Thakral
authors.forename: Neelam
authors.initials: N
authors.lastname: Marwaha
authors.forename: Y K
authors.initials: YK
authors.lastname: Chawla
authors.forename: Karan
authors.initials: K
authors.lastname: Saluja
authors.forename: Arpita
authors.initials: A
authors.lastname: Sharma
authors.forename: R R
authors.initials: RR
authors.lastname: Sharma
authors.forename: R W
authors.initials: RW
authors.lastname: Minz
authors.forename: S K
authors.initials: SK
authors.lastname: Agnihotri
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D001782:Blood Donors; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification; D006526:Hepatitis C / Q000453:epidemiology* / Q000276:immunology / Q000635:transmission; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D007194:India / Q000453:epidemiology; D008875:Middle Aged; D011446:Prospective Studies; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D016036:Seroepidemiologic Studies; D065227:Transfusion Reaction
chemical_list: D018937:Hepatitis C Antibodies; D012367:RNA, Viral