RALBP1/RLIP76 mediates multidrug resistance.
RLIP76/RALBP1 is a multi-specific drug-transporter which can mediate drug-resistance in lung and other cancers, but its ability to mediate multidrug-resistance has not been previously demonstrated in hematological malignancy. Present studies in K562 human myelogenous leukemia show that RALBP1 overexpression confers broad resistance to multiple chemotherapy drugs including cisplatin, melphalan, doxorubicin, daunorubicin, vincristine, vinblastine, vinorelbine, and mitomycin-C. Conversely, inhibition of RALBP1 by polyclonal antibodies causes increased drug-accumulation and increased cytotoxicity. These studies demonstrate the potential utility of targeting RALBP1 in the treatment of leukemia.
Drake
Kenneth J
KJ
Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX 76019-0065, USA.
Singhal
Jyotsana
J
Yadav
Sushma
S
Nadkar
Aalok
A
Pungaliya
Chirag
C
Singhal
Sharad S
SS
Awasthi
Sanjay
S
D018528:ATP-Binding Cassette Transporters / Q000276:immunology / Q000502:physiology*; D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000494:pharmacology; D018432:Drug Resistance, Multiple; D020690:GTPase-Activating Proteins / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D020014:K562 Cells; D007700:Kinetics; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
D018528:ATP-Binding Cassette Transporters; D000970:Antineoplastic Agents; D020690:GTPase-Activating Proteins; D007074:Immunoglobulin G; C095637:RALBP1 protein, human; D011994:Recombinant Proteins