A ligase ribozyme obtained from a structured pool.
Here we report the in vitro selection experiment employed to construct a catalytic site on a self-folding RNA. To practice the selection, the sequence of 30 nucleotides was located in close proximity of the putative reaction site in a derivative of a naturally occurring RNA with a well known 3D structure. The acquired catalytic RNA retained the starting 3D structure and was successfully reconstructed according to its modular structure.
Yoshioka
Wataru
W
Department of Chemistry, Graduate School of Science, Kyoto University, Japan.
Ikawa
Yoshiya
Y
Jaeger
Luc
L
Inoue
Tan
T
D000818:Animals; D001483:Base Sequence; D008025:Ligases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D013768:Tetrahymena / Q000201:enzymology*
D016337:RNA, Catalytic; D008025:Ligases