title: [Establishment of autologous endometrial coculture and sequential system for human early embryo culture].
abstract: OBJECTIVE
  To establish a coculture and a sequential system for human early embryo culture.
  
  
  METHODS
  The endometrial tissue was digested enzymatically and cultured to achieve generated and cryo-thawed endometrial monolayer cells. The generated and cryo-thawed monolayer cells were cocultured with human 2PN embryos and transferred to sequential medium every 48 hours.
  
  
  RESULTS
  Human endometrial cells had viability in vitro culture. The autologously generated and cryo-thawed monolayer cells were successfully obtained, and 74.04% of the cryo-thawed cells were successfully used in coculturing human early embryos. The embryos developed well, with the clinical pregnancy rate of 68.83% and the implantation rate of 44.23%.
  
  
  CONCLUSIONS
  The autologous endometrial cell coculture and sequential culture system for human early embryo development provides a feasible method for studying human embryo development and implantation so as to improve embryo quality.
authors.affiliation: Reproductive Medicine Center, Drum Tower Hospital Affiliated to Nanjing University Medical College, Nanjing, Jiangsu 210008, China.
authors.forename: Ning-yuan
authors.initials: NY
authors.lastname: Zhang
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Hu
authors.forename: Hai-xiang
authors.initials: HX
authors.lastname: Sun
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Zhi-peng
authors.initials: ZP
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
mesh_terms: D002478:Cells, Cultured; D018920:Coculture Techniques; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology*; D004717:Endometrium / Q000166:cytology*; D005260:Female; D006801:Humans
chemical_list: D003470:Culture Media