Cry-consensus peptide, a novel peptide for immunotherapy of Japanese cedar pollinosis, induces Th1-predominant response in Cry j 1-sensitized B10.S mice.
Cry-consensus peptide (CCP) is a newly designed peptide for peptide-based immunotherapy of Japanese cedar pollinosis but its mechanism of efficacy is unknown. We investigated the effect of CCP on Cry j 1-specific Th1/Th2 response in a mice model. Subcutaneous injection of CCP decreased Cry j 1-specific IgE and IgG1 in blood slightly, but the IgG2a level was increased significantly in a dose dependent manner. Splenocytes from these mice were stimulated with Cry j 1 in vitro. This inhibited IL-4, IL-5 and IL-10 secretion significantly, but IFN-gamma secretion was increased. In vitro CCP stimulation of splenocytes from Cry j 1-sensitized mice induced more marked Th1-predominancy of cytokine production than native allergen stimulation. Taken together, these data suggest that one of the mechanisms of CCP is dependent on the modulation of the antigen-specific Th1/Th2 response.
Kozutsumi
Daisuke
D
Research and Development Section, Pharmaceuticals Development Department, Meiji Dairies Corporation, Kanagawa, Japan. daisuke_kozutsumi@meiji-milk.com
Tsunematsu
Masako
M
Yamaji
Taketo
T
Murakami
Rika
R
Yokoyama
Minehiko
M
Kino
Kohsuke
K
D000485:Allergens / Q000008:administration & dosage / Q000009:adverse effects*; D000818:Animals; D052179:Antigens, Plant; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007167:Immunotherapy; D051379:Mice; D010455:Peptides / Q000276:immunology / Q000627:therapeutic use*; D010940:Plant Proteins / Q000008:administration & dosage / Q000009:adverse effects*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology / Q000628:therapy*; D018417:Th1 Cells / Q000276:immunology*
D000485:Allergens; D052179:Antigens, Plant; C065290:Cry j I protein, Cryptomeria japonica; D016207:Cytokines; D010455:Peptides; D010940:Plant Proteins