title: N-methyl-D-aspartate receptor type 1 immunoreactivity and protein level in the gerbil main olfactory bulb after transient forebrain ischemia.
abstract: It has been reported that the over-stimulation of N-methyl-D-aspartate receptor (NR) modulates glutamate postsynaptic neurotransmission by generating long lasting Ca2+ channel openings. In the present study, we investigated ischemia-induced change in NR1 immunoreactivity and level in the main olfactory bulb (MOB) after 5 min of transient forebrain ischemia in gerbils. NR1 immunoreactivity in the sham-operated group was shown mainly in tufted cells of the external plexiform and in mitral cells of the mitral cell layer. NR1 immunoreactivity in these neurons was increased with time and was very strong 15 days after ischemia/reperfusion. At that time, NR1 protein level in the MOB was also highest. Thereafter, NR1 immunoreactivity and protein level in the MOB were decreased with time after ischemia/reperfusion. Thus, NR1 in tufted and mitral cells in the gerbil MOB is changed after transient forebrain ischemia. This suggests that mitral and tufted cells may be the principal neurons in the MOB affected in receiving inputs and sending projections to the olfactory area after transient ischemia.
authors.affiliation: Department of Training & Education, Kandong Sacred Heart Hospital, Hallym University, Seoul 134-701, South Korea.
authors.forename: Young
authors.initials: Y
authors.lastname: Her
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Jae Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Bong-Hee
authors.initials: BH
authors.lastname: Lee
authors.forename: Do Hoon
authors.initials: DH
authors.lastname: Kim
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005849:Gerbillinae; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000503:physiopathology*; D008297:Male; D009830:Olfactory Bulb / Q000378:metabolism*; D016548:Prosencephalon / Q000098:blood supply / Q000166:cytology; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D015427:Reperfusion Injury / Q000503:physiopathology
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate