title: HBV and HCV infections and acute rejection differentially modulate CD95 and CD28 expression on peripheral blood lymphocytes after liver transplantation.
abstract: Hepatitis B virus (HBV) and hepatitis C virus (HCV) generally reinfect liver graft early posttransplantation and lead to poorer graft and patient survivals. In the present study the influence of acute rejection (AR), HBV and HCV infections, and human leukocyte antigen (HLA) class-I compatibility on the expression of CD28 (in 237 liver recipients) and CD95 (in 114 liver recipients) on peripheral blood cells were evaluated by flow cytometry during the first month after transplantation. HBV/HCV infections induced strong CD95 upregulation on CD3+ lymphocytes. Maximal CD95 upmodulation was found in infected recipients showing partial HLA class-I compatibility. AR and virus reinfection could be distinguished because CD28 was upregulated on CD4+ lymphocytes only in recipients with AR, irrespective of their status regarding HBV/HCV infections. In conclusion, cytometric co-evaluation of CD95 and CD28 expression on peripheral blood lymphocytes could be useful to discriminate AR from cellular activation induced by viral reinfection of the liver graft.
authors.affiliation: Immunology Services, Virgen de la Arrixaca University Hospital, Murcia, Spain. alfredo.minguela@carm.es
authors.forename: Alfredo
authors.initials: A
authors.lastname: Minguela
authors.forename: Manuel
authors.initials: M
authors.lastname: Miras
authors.forename: Juan
authors.initials: J
authors.lastname: Bermejo
authors.forename: Francisco
authors.initials: F
authors.lastname: Sánchez-Bueno
authors.forename: María R
authors.initials: MR
authors.lastname: López-Alvarez
authors.forename: María R
authors.initials: MR
authors.lastname: Moya-Quiles
authors.forename: Manuel
authors.initials: M
authors.lastname: Muro
authors.forename: Jesús
authors.initials: J
authors.lastname: Ontañón
authors.forename: Ana M
authors.initials: AM
authors.lastname: Garía-Alonso
authors.forename: Pascual
authors.initials: P
authors.lastname: Parrilla
authors.forename: Maria R
authors.initials: MR
authors.lastname: Alvarez-López
mesh_terms: D015415:Biomarkers / Q000032:analysis; D018106:CD28 Antigens / Q000097:blood*; D005434:Flow Cytometry; D006084:Graft Rejection / Q000097:blood / Q000276:immunology*; D006509:Hepatitis B / Q000097:blood / Q000276:immunology*; D006526:Hepatitis C / Q000097:blood / Q000276:immunology*; D006648:Histocompatibility; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016867:Immunocompromised Host; D007963:Leukocytes, Mononuclear / Q000276:immunology; D016031:Liver Transplantation / Q000276:immunology*; D012189:Retrospective Studies; D019014:fas Receptor / Q000097:blood*
chemical_list: D015415:Biomarkers; D018106:CD28 Antigens; D015395:Histocompatibility Antigens Class I; D019014:fas Receptor