The structure of starch can be manipulated by changing the expression levels of starch branching enzyme IIb in rice endosperm.
When the starch branching enzyme IIb (BEIIb) gene was introduced into a BEIIb-defective mutant, the resulting transgenic rice plants showed a wide range of BEIIb activity and the fine structure of their amylopectins showed considerable variation despite having the two other BE isoforms, BEI and BEIIa, in their endosperm at the same levels as in the wild-type. The properties of the starch granules, such as their gelatinization behaviour, morphology and X-ray diffraction pattern, also changed dramatically depending on the level of BEIIb activity, even when this was either slightly lower or higher than that of the wild-type. The over-expression of BEIIb resulted in the accumulation of excessive branched, water-soluble polysaccharides instead of amylopectin. These results imply that the manipulation of BEIIb activity is an effective strategy for the generation of novel starches for use in foodstuffs and industrial applications.
Tanaka
Naoki
N
National Institute of Agrobiological Sciences, Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Fujita
Naoko
N
Nishi
Aiko
A
Satoh
Hikaru
H
Hosaka
Yuko
Y
Ugaki
Masashi
M
Kawasaki
Shinji
S
Nakamura
Yasunori
Y