title: [Adventitious root induction and in vitro culture of Panax notoginseng].
abstract: OBJECTIVE
  To investigate the induction and culture of adventitious root of Panax notoginseng.
  
  
  METHODS
  Three ways, induction from the explants of three-year-old P. notoginseng. The explants of regenerated shoots and calluses, were used to induce adventitious roots. The effects of 2, 4-dichlorophenoxyacetic acid, indole-3-butyric acid and naphthylacetic acid on adventitious root induction were investigated respectively. The effects of four modes of separating adventitious roots from the parent tissues on culture in vitro were compared.
  
  
  RESULTS
  Adventitious roots were successfully induced by three methods, of which the young flower bud callus was the best material for the induction of adventitious root. Indole-3-butyric acid possessed the strongest potency for induction. The liquid culture system was established by continuous culture of adventitious roots together with their parent tissues before separated.
  
  
  CONCLUSIONS
  The acquisition and culture in vitro in liquid culture system of adventitious roots of P. notoginseng lay a foundation for the next investigation.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Xian-Fu
authors.initials: XF
authors.lastname: Gao
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Xu
authors.forename: Jia-Jian
authors.initials: JJ
authors.lastname: Liu
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Ma
authors.forename: Long-Ping
authors.initials: LP
authors.lastname: Yin
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D053723:Panax notoginseng / Q000187:drug effects / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D007211:Indoles; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; C014612:indolebutyric acid; D015084:2,4-Dichlorophenoxyacetic Acid; C034182:1-naphthaleneacetic acid