Interaction of non-steroidal anti-inflammatory drugs, etoricoxib and parecoxib sodium, with human serum albumin studied by fluorescence spectroscopy.
The mechanism of interaction of the non-steroidal anti-inflammatory drugs, etoricoxib and parecoxib sodium, with human serum albumin (HSA) was studied using fluorescence spectroscopy. There was only one class of binding site with association constants of the order of 10(4). Thermodynamic parameters suggest that van der Waals and hydrogen bonding interactions in the case of etoricoxib, and electrostatic and hydrogen bonding interactions in the case of parecoxib sodium, are predominantly involved in the binding. Studies in the presence of the hydrophobic probe, 1-anilinonaphthalene-8-sulfonate (ANS), showed that hydrophobic interactions are not involved in the binding of these drugs to HSA. Displacement studies using the site-specific probe, dansylsarcosine piperidinium salt (DSS), showed that the drugs are bound at site II on the HSA molecule. However, etoricoxib and parecoxib sodium are bound at different regions within site II. Increase of pH and the presence of salt caused significant changes in the association constants and the concentration of free pharmacologically active drug. Stern-Volmer analysis of the binding data indicated that the tryptophan residues of albumin are not fully accessible to anionic parecoxib sodium and a predominantly static quenching mechanism is operative in each case.
Seedher
Neelam
N
Department of Chemistry, Panjab University, Chandigarh, India. nseedher@yahoo.com
Bhatia
Sonu
S
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism*; D001665:Binding Sites; D004347:Drug Interactions; D000077613:Etoricoxib; D006801:Humans; D007555:Isoxazoles / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013450:Sulfones / Q000378:metabolism*; D013816:Thermodynamics
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007555:Isoxazoles; D011725:Pyridines; D012709:Serum Albumin; D013450:Sulfones; C409945:parecoxib; D000077613:Etoricoxib