title: Mapping the proteome of poplar and application to the discovery of drought-stress responsive proteins.
abstract: Poplar is the first forest tree genome to be decoded. As an initial step to the comprehensive analysis of poplar proteome, we described reference 2-D-maps for eight tissues/organs of the plant, and the functional characterization of some proteins. A total of 398 proteins were excised from the gels. About 91.2% were identified by nanospray LC-MS/MS, based on comparison with 260,000 Populus sp. ESTs. In comparison, reliable PMFs were obtained for only 51% of the spots by MALDI-TOF-MS, from which 43% (83 spots) positively matched gene models of the Populus trichocarpa genome sequence. Among these 83 spots, 58% matched with the same proteins as identified by LC-MS/MS, 21.7% with unknown function proteins and 19.3% with completely different functions. In the second phase, we studied the effect of drought stress on poplar root and leaf proteomes. The function of up- and down-regulated proteins is discussed with respect to the physiological response of the plants and compared with transcriptomic data. Some important clues regarding the way poplar copes with water deficit were revealed.
authors.affiliation: UMR Biodiversité Gènes Communautés, INRA, Equipe de génétique, Cestas, France. plomion@pierroton.inra.fr
authors.forename: Christophe
authors.initials: C
authors.lastname: Plomion
authors.forename: Céline
authors.initials: C
authors.lastname: Lalanne
authors.forename: Stéphane
authors.initials: S
authors.lastname: Claverol
authors.forename: Hakim
authors.initials: H
authors.lastname: Meddour
authors.forename: Annegret
authors.initials: A
authors.lastname: Kohler
authors.forename: Marie-Béatrice
authors.initials: MB
authors.lastname: Bogeat-Triboulot
authors.forename: Aurélien
authors.initials: A
authors.lastname: Barre
authors.forename: Grégoire
authors.initials: G
authors.lastname: Le Provost
authors.forename: Hélène
authors.initials: H
authors.lastname: Dumazet
authors.forename: Daniel
authors.initials: D
authors.lastname: Jacob
authors.forename: Catherine
authors.initials: C
authors.lastname: Bastien
authors.forename: Erwin
authors.initials: E
authors.lastname: Dreyer
authors.forename: Antoine
authors.initials: A
authors.lastname: de Daruvar
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Guehl
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Schmitter
authors.forename: Francis
authors.initials: F
authors.lastname: Martin
authors.forename: Marc
authors.initials: M
authors.lastname: Bonneu
mesh_terms: D030562:Databases, Protein; D004190:Disasters; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D020411:Oligonucleotide Array Sequence Analysis; D010449:Peptide Mapping / Q000379:methods; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D032107:Populus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods* / Q000706:statistics & numerical data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D010940:Plant Proteins