title: Protein kinase C-alpha and -delta are required for NADPH oxidase activation in WKYMVm-stimulated IMR90 human fibroblasts.
abstract: The regulation of the activation of non phagocytic NADPH oxidase is poorly understood. Previously we demonstrated that in fibroblasts the exposure to WKYMVm induced p47(phox) phosphorylation and translocation and that these effects were mediated by ERKs activation. Protein kinase C (PKC) is reported to be involved in regulating the phosphorylation of NADPH oxidase components in polymorphonucleate cells stimulated via FPRL1 receptor, but its involvement in fibroblasts was not demonstrated. Therefore, we investigated in IMR90 cells exposed to WKYMVm the role of PKC isoenzymes in the activation of NADPH oxidase-like enzyme. Preincubation with general pharmacological inhibitors of PKC, before stimulation with WKYMVm, prevented the ERKs activation, p47(phox) phosphorylation and translocation. The analysis of cellular partitioning of PKC isoenzymes demonstrated that PKCalpha and PKCdelta translocated from the cytosolic to the membrane fraction upon stimulation with WKYMVm. Preincubation with Gö6976 or with rottlerin prevented the phosphorylation and translocation of NADPH oxidase regulatory subunit.
authors.affiliation: CEINGE-Biotecnologie Avanzate s.c.a.r.l., Naples, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Iaccio
authors.forename: Claudio
authors.initials: C
authors.lastname: Collinet
authors.forename: Nicola Montesano
authors.initials: NM
authors.lastname: Gesualdi
authors.forename: Rosario
authors.initials: R
authors.lastname: Ammendola
mesh_terms: D002460:Cell Line; D016895:Culture Media, Serum-Free; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D019255:NADPH Oxidases / Q000378:metabolism*; D009842:Oligopeptides / Q000008:administration & dosage*; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D051745:Protein Kinase C-delta / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
chemical_list: D016895:Culture Media, Serum-Free; D009842:Oligopeptides; C113617:Trp-Lys-Tyr-Met-Val-Met; D019255:NADPH Oxidases; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta