Design of sensing ribonucleopeptides for small ligands.
Here we report a simple method to convert synthetic receptors to fluorescent sensors. An RNA-peptide complex (ribonucleopeptide) with a known three-dimensional structure is used as a framework of the receptor. Artificial ribonucleopeptide sensors were created with a combination of in vitro selection method and successive modification of the peptide with a fluorophore. A ribonucleopeptide complex of the fluorophore labeled peptide showed a remarkable fluorescence emission change upon binding cognate ligands.
Hagihara
Masaki
M
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
Hasegawa
Tetsuya
T
Tanabe
Yukiko
Y
Sato
Shin-ichi
S
Yoshikawa
Susumu
S
Ohkubo
Katsutoshi
K
Morii
Takashi
T
D000588:Amines / Q000737:chemistry; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D008024:Ligands; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000032:analysis* / Q000737:chemistry*; D012261:Ribonucleoproteins / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics
D000588:Amines; D005456:Fluorescent Dyes; D008024:Ligands; D010455:Peptides; D012261:Ribonucleoproteins