title: A sensitive method for quantitation of beta-lyase metabolites of sulfur mustard as 1,1'-sulfonylbis[2-(methylthio)ethane] (SBMTE) in human urine by isotope dilution liquid chromatography-positive ion-electrospray-tandem mass spectrometry.
abstract: A method for measurement of an important biological marker, 1,1'-sulfonylbis[2-(methylthio)ethane] (SBMTE) of sulfur mustard agent HD [bis-(2-chloroethyl)sulfide] in human urine, to quantify HD exposure, is presented. It employs TiCl3 reduction of beta-lyase metabolites to SBMTE, and automated solid-phase extraction sample preparation, followed by isotope dilution liquid chromatography-positive ion-electrospray ionization-tandem mass spectrometry with 7.5 min/sample cycle time, to achieve SBMTE quantitation of up to 200 samples/24h a day. Percent relative standard deviations over the calibration range varied from 12.0% at 0.1 ng/mL to 0.9% at 100 ng/mL, and the limit of detection from a 0.5 mL sample was below the lowest level calibration standard of 0.1 ng/mL.
authors.affiliation: Wadsworth Center for Laboratories and Research, New York State Department of Health, Empire State Plaza, Albany, NY 12201-0509, USA.
authors.forename: James D
authors.initials: JD
authors.lastname: Daly
authors.forename: Colleen M
authors.initials: CM
authors.lastname: O'Hehir
authors.forename: George M
authors.initials: GM
authors.lastname: Frame
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007554:Isotopes; D008190:Lyases / Q000378:metabolism*; D009151:Mustard Gas / Q000378:metabolism*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013450:Sulfones / Q000652:urine*; D013454:Sulfoxides / Q000652:urine*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007554:Isotopes; D013450:Sulfones; D013454:Sulfoxides; C073452:1,1'-sulfonylbis(2-(methylsulfinyl)ethane); D008190:Lyases; D009151:Mustard Gas