Molecular shift register and its utilization as an autonomous DNA synthesizer.
A novel algorithmic approach to the synthesis of fairly long DNA molecules with nonrecurring sequences is demonstrated. The scheme exploits chemical embodiment of shift registers (SR) to execute algorithms similar to those used to generate pseudorandom numbers on a computer. Single stranded DNA molecules guide the synthesis of double stranded DNA according to the SR truth table. The SR logic facilitates an exponentially smaller synthesis effort compared with all other strategies. A redundancy based scheme, similar to those used in communication, is utilized to suppress synthesis errors.
Baskin
Ilya
I
Department of Physics, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Zaitsev
Stav
S
Lipson
Doron
D
Gilad
Rachel
R
Keren
Kinneret
K
Ben-Yoseph
Gidi
G
Sivan
Uri
U
D000465:Algorithms; D001483:Base Sequence; D001703:Biophysics / Q000379:methods*; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry; D004261:DNA Replication; D015723:Gene Library; D007700:Kinetics; D008954:Models, Biological; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013816:Thermodynamics
D017931:DNA Primers; D004247:DNA