Chemical modifications rescue off-target effects of RNAi.
RNAi's specificity has been questioned for some time. Three recent papers show that off-target effects should be considered normal, but one paper also provides insight on how chemical modifications of siRNAs may overcome the problem.
Snøve
Ola
O
Division of Molecular Biology, Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, California 91010, USA.
Rossi
John J
JJ
D000818:Animals; D018390:Gene Targeting / Q000379:methods*; D006801:Humans; D034622:RNA Interference / Q000502:physiology*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics*
D034741:RNA, Small Interfering