Influence of culture conditions and virulence plasmids on expression of immunoglobulin-binding proteins of Yersinia pseudotuberculosis.
The influence of culture conditions and plasmids on immunoglobulin (Ig)-binding activity of two isogenic strains of Yersinia pseudotuberculosis (plasmid-free strain 48(-)82(-) and strain 48(+)82(+) bearing plasmids pYV48 and pVM82) was studied. The highest activity was observed in the bacteria grown on glucose-containing liquid medium in the stationary growth phase. The Ig-binding activity of the bacteria cultured on the liquid medium at pH 6.0 was about 1.5-fold higher than that of the bacteria grown at pH 7.2. Expression of the Ig-binding proteins (IBPs) was most influenced by temperature of cultivation. The IBP biosynthesis was activated in the bacteria grown at 4 degrees C and markedly decreased in those grown at 37 degrees C. The Ig-binding activity of lysates from the bacteria was caused by proteins with molecular weights of 7-20 kD. The activities of the plasmid-free and plasmid-bearing Y. pseudotuberculosis strains (48(-)82(-) and 48(+)82(+), respectively) were analyzed, and the plasmids were shown to have no effect on the IBP expression and biosynthesis, which seemed to be determined by chromosomal genes.
Naberezhnykh
G A
GA
Pacific Institute of Bioorganic Chemistry, Far-Eastern Division, Russian Academy of Sciences, 690022 Vladivostok, Russia. naber1953@mail.ru
Sidorin
E V
EV
Lapshina
L A
LA
Reunov
A V
AV
Solov'eva
T F
TF
D000818:Animals; D003470:Culture Media / Q000494:pharmacology*; D005502:Food; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000378:metabolism; D008222:Lymphokines / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000502:physiology; D011817:Rabbits; D037521:Virulence Factors / Q000502:physiology*; D015011:Yersinia pseudotuberculosis / Q000254:growth & development* / Q000378:metabolism*
D003470:Culture Media; D007074:Immunoglobulin G; D008222:Lymphokines; D037521:Virulence Factors; C013059:immunoglobulin-binding factors