DNA secondary structure forming at minisatellite repeat unit sequences.
The lengths of simple repeat sequences are generally unstable or polymorphic; that is, they are highly variable with respect to the numbers of tandem repeats. To determine the structural features that cause such variability, we examined a minisatellite DNA isolated from the yellow fin sea bream Acanthopagrus latus. Electrophoresis, CD spectra, and calorimetric analyses of oligodeoxyribonucleotides comprised of the minisatellite repeat unit suggest a stable secondary structure is formed within the repeat unit.
Haku
Takeshi
T
Department of Biological Science, Osaka Prefecture University Graduate School of Science, Sakai 599-8531, Japan.
Hibino
Takeshi
T
Fukada
Harumi
H
Mishima
Yumiko
Y
Yamashita
Ichiro
I
Kato
Mikio
M
D000818:Animals; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D018598:Minisatellite Repeats; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010473:Perciformes; D012984:Software; D013696:Temperature
D009838:Oligodeoxyribonucleotides; D004247:DNA