Molecular epidemiology of adenoviral conjunctivitis in Hanoi, Vietnam.
OBJECTIVE
To investigate the serotypes of adenovirus causing conjunctivitis in Hanoi, Vietnam.


METHODS
Clinical diagnosis of adenoviral conjunctivitis and laboratory-based experimental study.


METHODS
We collected 21 conjunctival swabs from 21 different patients with a clinical presentation compatible with adenoviral conjunctivitis, in Hanoi, Vietnam. Immunochromatography and real-time polymerase chain reaction (PCR) were performed to detect human adenovirus (HAdV). The sequence of PCR products was analyzed to determine the serotype of HAdV.


RESULTS
Of 21 samples, HAdV DNA was detected in 14 samples (66.7%) by real-time PCR. The serotype analysis showed HAdV-8 in 11 samples (78.6%), HAdV-3 in two samples (14.3%), and HAdV-37 in one sample (7.1%). Of 11 HAdV-8 samples, one sample (9.1%) was prototype, and the other 10 samples (90.9%) had identical nucleotide sequence and were identified as a variant of HAdV-8.


CONCLUSIONS
HAdV-8 was found to be the predominant serotype in Hanoi, Vietnam. Most of the HAdV-8 samples were a variant of HAdV-8.
Jin
Xue-Hai
XH
Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo, Japan. xhjin@med.hokudai.ac.jp
Ishiko
Hiroaki
H
Nguyen
Thanh Ha
TH
Ohguchi
Takeshi
T
Akanuma
Masataka
M
Aoki
Koki
K
Ohno
Shigeaki
S
D000258:Adenovirus Infections, Human / Q000453:epidemiology* / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics*; D002845:Chromatography; D003228:Conjunctiva / Q000821:virology; D003236:Conjunctivitis, Viral / Q000453:epidemiology* / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D007158:Immunologic Techniques; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D014744:Vietnam / Q000453:epidemiology
D004279:DNA, Viral