title: Time-dependent modulation of rat serum paraoxonase 1 activity by fasting.
abstract: High-density lipoprotein-associated paraoxonase 1 (PON1) protects the endothelium from the pro-oxidant activity of oxidised low-density lipoprotein. Whereas fasting has been related to increased oxidative stress, intermittent fasting and caloric restriction are associated to increased resistance to oxidative injury. Taking into consideration that serum PON1 activity is modulated by a restriction of caloric intake and because there is no evidence regarding PON1 response to total food deprivation, we investigated whether PON1 activity is involved in the response aimed to counteract the greater oxidative stress associated to fasting and whether serum PON1 activity is altered by the length of food deprivation. Male Wistar rats were randomly divided into five groups: fed and 6-, 12-, 24- or 48-h fasted rats. Serum PON1 activity increases within the first hours of fasting, representing a prompt adaptation designed to attenuate blood lipid peroxidation that cannot be sustained when fasting is prolonged. This PON1 response to early fasting could be part of the mechanisms triggered by periodically repeated short periods of food deprivation - intermittent fasting - which result in increased resistance to stress by stimulating antioxidant defences.
authors.affiliation: Grup de Metabolisme Energètic i Nutrició, Departament de Biologia Fonamental i Ciències de la Salut, Institut Universitari d'Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, Palma de Mallorca, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Thomàs-Moyà
authors.forename: Antònia
authors.initials: A
authors.lastname: Nadal-Casellas
authors.forename: Magdalena
authors.initials: M
authors.lastname: Gianotti
authors.forename: Isabel
authors.initials: I
authors.lastname: Lladó
authors.forename: Ana María
authors.initials: AM
authors.lastname: Proenza
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood; D043303:Aryldialkylphosphatase / Q000097:blood* / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000097:blood; D002374:Catalase / Q000378:metabolism; D051152:Clusterin / Q000097:blood; D005215:Fasting / Q000502:physiology*; D005508:Food Deprivation / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology*; D008099:Liver / Q000033:anatomy & histology / Q000201:enzymology; D008297:Male; D009929:Organ Size; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051122:Scavenger Receptors, Class B / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
chemical_list: D016632:Apolipoprotein A-I; C498500:Clu protein, rat; D051152:Clusterin; D012333:RNA, Messenger; C495125:Scarb1 protein, rat; D051122:Scavenger Receptors, Class B; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase