Tomographic molecular imaging and 3D quantification within adult mouse organs.
A convenient technology to quantify three-dimensional (3D) morphological features would have widespread applications in biomedical research. Based on combined improvements in sample preparation, tomographic imaging and computational processing, we present a procedure for high-resolution 3D quantification of structures within intact adult mouse organs. Using the nonobese diabetic (NOD) mouse model, we demonstrate a correlation between total islet beta-cell volume and the onset of type-1 diabetes.
Alanentalo
Tomas
T
Umeå Center for Molecular Medicine, Umeå University, S-901 87, Umeå, Sweden.
Asayesh
Amir
A
Morrison
Harris
H
Lorén
Christina E
CE
Holmberg
Dan
D
Sharpe
James
J
Ahlgren
Ulf
U
D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000175:diagnosis* / Q000503:physiopathology; D004195:Disease Models, Animal; D018450:Disease Progression; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007515:Islets of Langerhans / Q000473:pathology*; D051379:Mice; D010179:Pancreas / Q000473:pathology*; D012680:Sensitivity and Specificity; D014054:Tomography / Q000379:methods*