title: Synthesis and properties of novel base-discriminating fluorescent (BDF) nucleosides.
abstract: We designed a new type of pyrene-labeled base-discrimination fluorescent (BDF) nucleosides (Py)U, (Py)C, (8Py)A and (MePy)dA, which emitted strong fluorescence only when the bases opposite the BDF base are A, G, T and C, respectively. The DNA probes containing four different BDF bases enable us to distinguish single base alterations by simply mixing with a sample solution of target DNA.
authors.affiliation: NEWCAT Institue, School of Engineering, Nihon University and SORST, Japan Science and Tecnology Agency, Tamura, Koriyama, 963-8642, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Saito
authors.forename: Kazuo
authors.initials: K
authors.lastname: Hanawa
authors.forename: Keigo
authors.initials: K
authors.lastname: Hayashi
authors.forename: Kaori
authors.initials: K
authors.lastname: Motegi
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okaoto
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
mesh_terms: D000241:Adenosine / Q000138:chemical synthesis / Q000737:chemistry; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D009705:Nucleosides / Q000138:chemical synthesis / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D009705:Nucleosides; D011721:Pyrenes; C030984:pyrene; D000241:Adenosine