Functional analysis of factors involved in trans-translation.
trans-translation is a rescue system for stalled ribosomes to terminate the translation for recycling of ribosome and lead the incomplete protein on the stalled ribosome to degradation. In trans-translation, several proteins and RNAs are involved. tmRNA and SmpB are only factors known to be essential for trans-translation. EF-Tu and EF-G functions in translation and also are seemed to function in trans-translation. Ribosomal protein S1 is known to bind tmRNA. In this study, we cloned the gene of tmRNA from Thermus thermophilus and analyzed the functions of factors involved in trans-translation in vitro.
Takada
Kazuma
K
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, 036-8561, Japan.
Takemoto
Chie
C
Kawazoe
Masahito
M
Konno
Takayuki
T
Matsuta
Noriko
N
Kurita
Daisuke
D
Asano
Krisana
K
Shirouzu
Mikako
M
Yokoyama
Shigeyuki
S
Muto
Akira
A
Himeno
Hyouta
H
D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010303:Paromomycin / Q000494:pharmacology; D010443:Peptide Chain Termination, Translational; D012329:RNA, Bacterial / Q000737:chemistry* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D016963:Thermus thermophilus / Q000235:genetics
D012329:RNA, Bacterial; D012269:Ribosomal Proteins; C096222:tmRNA; D010303:Paromomycin