Creation of superior carboxyfluorescein dyes by blocking donor-excited photoinduced electron transfer.
[Structure: see text] Carboxyfluoresceins are widely utilized as fluorescence labeling reagents, but we recently found that their emission intensity is markedly decreased after esterification. On the basis of our hypothesis that the fluorescence decrease is due to a donor-excited photoinduced electron transfer (d-PeT) process, we have developed novel carboxyfluorescein derivatives in which the d-PeT process is hampered, and the emission intensity is not decreased upon esterification. These novel dye derivatives display high quantum yields and are expected to be useful as labeling agents.
Mineno
Tomoko
T
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Ueno
Tasuku
T
Urano
Yasuteru
Y
Kojima
Hirotatsu
H
Nagano
Tetsuo
T
D004396:Coloring Agents / Q000737:chemistry*; D004583:Electrons; D005452:Fluoresceins / Q000737:chemistry*; D010777:Photochemistry
D004396:Coloring Agents; D005452:Fluoresceins; C024098:6-carboxyfluorescein