title: Isolation of 151 mutants that have developmental defects from T-DNA tagging.
abstract: In order to understand the mechanisms underlying plant development, a necessary first step involves the elucidation of the functions of the genes, via the analysis of mutants that exhibit developmental defects. In this study, an activation tagging mutant library harboring 80,650 independent Arabidopsis transformants was generated in order to screen for developmental mutants. A total of 129 mutants manifesting dominant developmental abnormalities were isolated, and their T-DNA insertion loci were mapped. The activation of one or more genes adjacent to a T-DNA insertion locus was confirmed in eight dominant mutants. A gene adjacent to the right border was usually activated by the 35S enhancers. Interestingly, the transcriptional activation of multiple genes within a broad range was observed in one of the mutants, which raises the possibility that activation by the 35S enhancers was not limited strictly to a single gene. In order to gain a better understanding of sexual reproduction in higher plants, we isolated 22 mutants exhibiting defects in female gametophyte development, and determined their T-DNA insertion loci. We propose that this mutant population may prove useful in the further determination of the functions of genes that play important roles in plant development.
authors.affiliation: Plant Signaling Network Research Center, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Ahn
authors.forename: Joonki
authors.initials: J
authors.lastname: Kim
authors.forename: Seong Jeon
authors.initials: SJ
authors.lastname: Yoo
authors.forename: So Yeon
authors.initials: SY
authors.lastname: Yoo
authors.forename: Hyungmin
authors.initials: H
authors.lastname: Roh
authors.forename: Jun-Hyuk
authors.initials: JH
authors.lastname: Choi
authors.forename: Mi Suk
authors.initials: MS
authors.lastname: Choi
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Chung
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Han
authors.forename: Sung Myun
authors.initials: SM
authors.lastname: Hong
authors.forename: Sung Hye
authors.initials: SH
authors.lastname: Jung
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Kang
authors.forename: Bo Kyung
authors.initials: BK
authors.lastname: Kim
authors.forename: Mi Duk
authors.initials: MD
authors.lastname: Kim
authors.forename: Youn Kyung
authors.initials: YK
authors.lastname: Kim
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Hanna
authors.initials: H
authors.lastname: Lee
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Jee Hoon
authors.initials: JH
authors.lastname: Yang
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Yang
authors.forename: Dong-Hun
authors.initials: DH
authors.lastname: Yoo
authors.forename: Seung Kwan
authors.initials: SK
authors.lastname: Yoo
authors.forename: Jong Seob
authors.initials: JS
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics*; D035264:Flowers / Q000235:genetics; D005784:Gene Amplification; D008969:Molecular Sequence Data; D009154:Mutation; D018515:Plant Leaves / Q000235:genetics; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; C036483:T-DNA