Expression of gamma-tubulin during the development of nematode Caenorhabditis elegans.
Gamma-tubulin is a centrosomal protein found in microtubule organizing centres (MTOCs) in cells from many different organisms, and has several properties, which makes it a candidate for both the initiation of microtubule assembly and anchorage. Gamma-tubulin is encoded by a single gene tbg-1 in Caenorhabditis elegans. In this paper tbg-1 was studied to understand the essential role of gamma-tubulin in C. elegans. Essential role of tbg-1 expression was confirmed by the disruption of the gene expression by gamma-tubulin anti-sense RNA production in vivo under the heat shock promoter that caused lethality in the nematodes. Expression of tbg-1 deduced from Northern blot analysis during the development revealed differential expression in different developmental stages. Using tbg-1::lacZ fusion gene expression studies in the germ line transformed worms, it was further revealed that gamma-tubulin expression was observed through out the development from embryonic and post-embryonic stages.
Tabish
Mohammad
M
Department of Material System Engineering, Toyohashi University of Technology, Toyohashi-Shi, Aichi-441, Japan. tabish.biochem@gmail.com
D000818:Animals; D015152:Blotting, Northern; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008957:Models, Genetic; D016372:RNA, Antisense / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014170:Transformation, Genetic; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; D016372:RNA, Antisense; D014404:Tubulin