Human PEP-1-ribosomal protein S3 protects against UV-induced skin cell death.
The consequences of ultraviolet (UV) exposure are implicated in skin aging and cell death. The ribosomal protein S3 (rpS3) is one of the major proteins by which cells counteract the deleterious effects of UV and it plays a role in the repair of damaged DNA. In the present study, we investigated the protective effects of PEP-1-rpS3 fusion protein after UV-induced cell injury. A human rpS3 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-rpS3 fusion protein. The expressed and purified fusion proteins were efficiently transduced into skin cells in a time- and dose-dependent manner. Once inside the cells, transduced PEP-1-rpS3 fusion protein was stable for 48h. We showed that transduced PEP-1-rpS3 fusion protein increased cell viability and dramatically reduced DNA lesions in the UV exposed skin cells. Immunohistochemical analysis revealed that PEP-1-rpS3 fusion protein efficiently penetrated the epidermis as well as the dermis of the subcutaneous layer when sprayed on animal skin. These results suggest that PEP-1-rpS3 fusion protein can be used in protein therapy for various disorders related to UV, including skin aging and cancer.
Choi
Soo Hyun
SH
Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Republic of Korea.
Kim
So Young
SY
An
Jae Jin
JJ
Lee
Sun Hwa
SH
Kim
Dae Won
DW
Ryu
Hea Jin
HJ
Lee
Nam Il
NI
Yeo
Seung Il
SI
Jang
Sang Ho
SH
Won
Moo Ho
MH
Kang
Tae-Cheon
TC
Kwon
Hyung Joo
HJ
Cho
Sung-Woo
SW
Kim
Joon
J
Lee
Kil Soo
KS
Park
Jinseu
J
Eum
Won Sik
WS
Choi
Soo Young
SY
D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D003543:Cysteamine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D004249:DNA Damage / Q000528:radiation effects; D005347:Fibroblasts; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D051379:Mice; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D012269:Ribosomal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D012867:Skin / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
C503924:Pep-1 peptide; D010455:Peptides; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; C044152:ribosomal protein S3; D003543:Cysteamine