Microarray analysis of stem cells and differentiation.
Microarrays have revolutionized molecular biology and enabled biologists to perform global analysis on the expression of tens of thousands of genes simultaneously. They have been widely used in gene discovery, biomarker determination, disease classification, and studies of gene regulation. Microarrays have been applied in stem cell research to identify major features or expression signatures that define stem cells and characterize their differentiation programs toward specific lineages. Here we provide a review of the microarray technology, including the introduction of array platforms, experimental designs, RNA isolation and amplification, array hybridization, and data analysis. We also detail examples that apply microarray technology to address several of the main questions in stem cell biology.
Chang
Howard Y
HY
Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.
Thomson
James A
JA
Chen
Xin
X
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D006801:Humans; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods; D021141:Nucleic Acid Amplification Techniques; D012313:RNA / Q000302:isolation & purification; D013234:Stem Cells / Q000166:cytology*
D012313:RNA