title: The OxyR regulon in nontypeable Haemophilus influenzae.
abstract: Nontypeable Haemophilus influenzae (NTHi) is a gram-negative bacterium and a common commensal organism of the upper respiratory tract in humans. NTHi causes a number of diseases, including otitis media, sinusitis, conjunctivitis, exacerbations of chronic obstructive pulmonary disease, and bronchitis. During the course of colonization and infection, NTHi must withstand oxidative stress generated by insult due to multiple reactive oxygen species produced endogenously by other copathogens and by host cells. Using an NTHi-specific microarray containing oligonucleotides representing the 1821 open reading frames of the recently sequenced NTHi isolate 86-028NP, we have identified 40 genes in strain 86-028NP that are upregulated after induction of oxidative stress due to hydrogen peroxide. Further comparisons between the parent and an isogenic oxyR mutant identified a subset of 11 genes that were transcriptionally regulated by OxyR, a global regulator of oxidative stress. Interestingly, hydrogen peroxide induced the OxyR-independent upregulation of expression of the genes encoding components of multiple iron utilization systems. This finding suggested that careful balancing of levels of intracellular iron was important for minimizing the effects of oxidative stress during NTHi colonization and infection and that there are additional regulatory pathways involved in iron utilization.
authors.affiliation: Columbus Children's Research Institute, Center for Microbial Pathogenesis, The Ohio State University College of Medicine and Public Health, 700 Children's Drive, Columbus, OH 43205-2696, USA.
authors.forename: Alistair
authors.initials: A
authors.lastname: Harrison
authors.forename: William C
authors.initials: WC
authors.lastname: Ray
authors.forename: Beth D
authors.initials: BD
authors.lastname: Baker
authors.forename: David W
authors.initials: DW
authors.lastname: Armbruster
authors.forename: Lauren O
authors.initials: LO
authors.lastname: Bakaletz
authors.forename: Robert S
authors.initials: RS
authors.lastname: Munson
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon; D011485:Protein Binding / Q000187:drug effects; D018085:Regulon; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D006861:Hydrogen Peroxide; D007501:Iron