Site-selective hydrolysis of huge DNA by artificial restriction DNA cutter.
Genomic DNAs of lambda phage (48.5 kbp) and E. coli (4.6 Mbp) were successfully cleaved at the target site by artificial restriction DNA cutter (ARCUT), which we recently developed by combining Ce(IV)/EDTA and PNA (peptide nucleic acid). PNA satisfactorily invaded the target site in such huge DNAs and activate there for Ce(IV)/EDTA-induced scission.
Yamamoto
Yoji
Y
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Miura
Kazuyuki
K
Komiyama
Makoto
M
D010582:Bacteriophage lambda / Q000235:genetics; D002563:Cerium / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism; D004492:Edetic Acid / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D006868:Hydrolysis; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D004269:DNA, Bacterial; D004279:DNA, Viral; D020135:Peptide Nucleic Acids; D002563:Cerium; D004492:Edetic Acid; D004262:DNA Restriction Enzymes