Synthesis of perylene-labeled base-discriminating fluorescent (BDF) nucleoside and its fluorescence properties.
We have synthesized perylene labeled BDF probe and its photophysical properties were explored. Perylene labeled oligonucleotide probe is capable of detecting cytosine from its target mismatch sequence by enhancement of fluorescence intensity.
Bag
Subhendu Sekhar
SS
Newcat Institute, School of Engineering, Nihon University, Tamura, Koriyama, Fukushima 963-8642, Japan.
Saito
Yoshio
Y
Hanawa
Kazuo
K
Hayasi
Keigo
K
Kodate
Satoshi
S
Suzuka
Isamu
I
Saito
Isao
I
D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D010569:Perylene / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014529:Uridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
C522090:2'-deoxy-5-(3-(3-perylenecarboxamido)propynyl)uridine; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D010569:Perylene; D014529:Uridine