Detection of 5-methylcytosine in DNA by photo-functionalized oligodeoxynucleotides possessing 2-methyl-1,4-naphthoquinone chromophore.
We developed a method for detection of 5-methylcytosine in DNA by photosensitized oxidation, using photo-functionalized oligodeoxynucleotides possessing 2-methyl-1,4-naphthoquinone chromophores (NQ) at the interior of the strand. The photooxidation and subsequent hot piperidine treatment of duplex consisting of the NQ-tethered oligodeoxynucleotide and its complementary strand bearing 5-methylcytosine residue led to selective DNA strand cleavage at the 5-methylcytosine residue, whereas no strand cleavage was detected for the control duplex with normal cytosine residue but not 5-methylcytosine.
Yamada
Hisatsugu
H
Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan.
Tanabe
Kazuhito
K
Nishimoto
Sei-ichi
S
D044503:5-Methylcytosine / Q000032:analysis*; D008027:Light; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000528:radiation effects; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA / Q000379:methods*; D013696:Temperature; D024483:Vitamin K 3 / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D044503:5-Methylcytosine; D024483:Vitamin K 3