A screening platform for compounds with potential immuno-regulatory activities using human cord blood mononuclear cells.
A systematic and combinatorial approach was adopted using human umbilical cord blood mononuclear cells (hUCB-MNCs) to screen for potential immuno-regulatory compounds. The hUCB-MNCs contain several types of immunogenic cells, which are a suitable material to mimic the in vivo immuno-response after drug treatment. hUCB-MNCs were treated with various natural products such as quercetin, astaxanthin, caffeic acid, bilobalide, eugenol, rutin and gamma-dodecalactone (gamma-DDL). Phenotypic expression analysis revealed that the subpopulation of CD3(+) T cells, CD56(+) NK cells and CD1a(+) dendritic cells apparently increased after being treated with gamma-DDL for 6 days. The expression of CD56 reached a maximum at 72 h with a dose-dependent relationship. The NK cells activation marker (CD69) also elevated following gamma-DDL treatment. These results demonstrated that the gamma-DDL has immuno-regulatory effects to enhance cord blood NK cells population and bioactivities. Such a high-throughput methodology using hUCB-MNCs may be an effective platform for systematically screening potential immuno-regulatory compounds.
Chen
Chia-Jung
CJ
Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan.
Tsai
Chia Che
CC
Hsieh
Jung-Feng
JF
Chien
Chichen Michael
CM
Wu
Tzu-Hua
TH
Chen
Shui-Tein
ST
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000494:pharmacology; D015703:Antigens, CD / Q000032:analysis* / Q000096:biosynthesis; D000954:Antigens, Surface / Q000032:analysis / Q000096:biosynthesis; D001685:Biological Factors / Q000494:pharmacology; D049109:Cell Proliferation; D002478:Cells, Cultured; D020650:Combinatorial Chemistry Techniques; D015195:Drug Design; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology*; D015394:Molecular Structure; D011247:Pregnancy
D015703:Antigens, CD; D000954:Antigens, Surface; D001685:Biological Factors; D015107:4-Butyrolactone; C517611:gamma-dodecalactone