Comparison of IgG antibody levels to Clostridium botulinum antigens between euthanased and surviving cases of chronic grass sickness.
Serum from 12 horses suffering from chronic grass sickness (CGS) were assayed for IgG antibodies against botulinum neurotoxins C and D (BoNT/C and BoNT/D) and to a surface antigen extract of a neurotoxin negative strain of Clostridium botulinum type C. Collectively, the six surviving CGS cases demonstrated significantly higher initial IgG levels (P=0.05) against surface antigens than the six that were subsequently euthanased. The surviving animals also demonstrated higher initial IgG levels against the BoNT/C but not reaching significance (P=0.06). The two groups demonstrated no difference between IgG levels against BoNT/D. This study supports existing evidence of the involvement of C. botulinum type C in the aetiology of grass sickness.
Nunn
F G
FG
Medical Microbiology, Centre for Infectious Diseases, University of Edinburgh College of Medicine and Veterinary Medicine, Edinburgh EH16 4SB, UK.
Pirie
R S
RS
McGorum
B
B
Wernery
U
U
Poxton
I R
IR
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology* / Q000378:metabolism; D001905:Botulinum Toxins / Q000276:immunology / Q000378:metabolism; D003014:Clostridium botulinum / Q000276:immunology*; D037901:Euthanasia, Animal; D006734:Horse Diseases / Q000276:immunology* / Q000382:microbiology*; D006736:Horses; D007074:Immunoglobulin G / Q000097:blood*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; C096325:botulinum toxin type D; D001905:Botulinum Toxins; C096324:botulinum toxin type C