title: Comparison of two different nutritive conditions against the changes in peripheral blood mononuclear cells of periparturient dairy cows.
abstract: To clarify the relationship between cellular immune status and nutritive condition in periparturient dairy cows, feeding content, blood profiles, and immune condition were observed in cows from two dairy herds with different types of feed content. Immunological analyses such as leukocyte population and peripheral blood mononuclear cell (PBMC) mRNA of IFN-gamma, TNF-alpha, IL-4, and IL-10, quantified by real-time RT-PCR were performed. With regard to feed content during dry periods, there were six cows in the herd with insufficient non-structural carbohydrate (NFC) intake (group I) and six cows in the herd with sufficient NFC intake (group II). Significantly lower levels of blood glucose were observed in group I between weeks -12 and 16 compared with group II. Serum cholesterol level was significantly lower in group I between weeks 2 and 10 than in group II. The numbers of CD3+ and CD4+ T cells in group I were significantly lower than those in group II in weeks 6 and 14. The numbers of CD21+ B cells were significantly lower in group I than in group II in weeks -16, -12, 2, and 10. On the other hand, the CD4+/CD8+ ratio in group II was significantly higher than group I between weeks 2 and 14. The IFNgamma/IL-4 mRNA rate in group I was significantly lower than group II in week 6. We concluded that cellular immune depression occurrs after calving in dairy cows with low nutritional status in the periparturient period.
authors.affiliation: School of Veterinary Medicine and Animal Sciences, Kitasato University, Aamori, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Chika
authors.initials: C
authors.lastname: Watanabe
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kohiruimaki
authors.forename: Takaaki
authors.initials: T
authors.lastname: Ando
authors.forename: Daisaku
authors.initials: D
authors.lastname: Watanabe
authors.forename: Machiko
authors.initials: M
authors.lastname: Masui
authors.forename: Tomohito
authors.initials: T
authors.lastname: Hayashi
authors.forename: Ryo
authors.initials: R
authors.lastname: Abe
authors.forename: Masateru
authors.initials: M
authors.lastname: Koiwa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sato
authors.forename: Seiichi
authors.initials: S
authors.lastname: Kawamura
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001786:Blood Glucose; D002417:Cattle / Q000276:immunology* / Q000502:physiology*; D016207:Cytokines / Q000235:genetics; D005260:Female; D007111:Immunity, Cellular / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D036801:Parturition / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D016207:Cytokines; D012333:RNA, Messenger