title: Anti-heat shock protein 90beta antibodies are detected in patients with multiple sclerosis during remission.
abstract: Antibodies against heat shock protein 90beta (Hsp90beta) recognize the antigen on the surface of oligodendrocyte precursor cells and induce a decrease in the oligodendrocyte population of cell cultures. This study investigated the presence of anti-Hsp90beta antibodies in patients with multiple sclerosis (MS) during remission. Anti-Hsp90beta antibodies were detected in the cerebrospinal fluid (CSF) of patients with a specific and sensitive western blot procedure using Hsp90beta from cell membrane fraction. The number of patients with anti-Hsp90beta antibodies detected in the CSF was significantly greater in MS patients than in control patients with other neurological diseases, which included patients with other inflammatory neurological diseases. CSF anti-Hsp90beta antibody levels were significantly higher in MS patients than in control patients. The presence of anti-Hsp90beta antibodies in the CSF of MS patients during remission could suggest a potential pathogenic role for these autoantibodies in MS.
authors.affiliation: Department of Investigation, Hospital Ramón y Cajal, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Cid
authors.forename: I
authors.initials: I
authors.lastname: Regidor
authors.forename: A
authors.initials: A
authors.lastname: Alcázar
mesh_terms: D000328:Adult; D000906:Antibodies / Q000134:cerebrospinal fluid*; D015153:Blotting, Western / Q000379:methods; D045744:Cell Line, Tumor; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid* / Q000276:immunology; D009447:Neuroblastoma / Q000473:pathology
chemical_list: D000906:Antibodies; D018841:HSP90 Heat-Shock Proteins; C493165:HSP90AB1 protein, human