title: A metalloproteinase gene from the pathogenic piscine hemoflagellate, Cryptobia salmositica.
abstract: We report the identification of a Cryptobia genomic DNA gene predicted to encode a hydrophobic protein containing a zinc metalloproteinase motif, HEXXH, and hence named it a major surface proteinase 1-like (MSP-1). The MSP-1 gene was identified using universal genome walking. Southern blot analysis revealed it to be a multicopy gene. Fragments of DNA encoding a segment homologous to the HEXXH motif of MSP-1 are widely found in bacteria, yeast, parasitic protozoans, plants, and animals including humans. These results suggest that the MSP-1 may have highly conserved functions, such as in intracellular proteolysis.
authors.affiliation: Department of Integrative Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada. jesudhasan@poultry.tamu.edu
authors.forename: Palmy R
authors.initials: PR
authors.lastname: Jesudhasan
authors.forename: Chung-Wei
authors.initials: CW
authors.lastname: Tan
authors.forename: Patrick T K
authors.initials: PT
authors.lastname: Woo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D056890:Eukaryota / Q000201:enzymology* / Q000235:genetics*; D005399:Fishes / Q000469:parasitology; D045726:Metalloproteases / Q000235:genetics*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D015800:Protozoan Proteins; D045726:Metalloproteases