Polymeric receptors for the recognition of folic acid and related compounds via substructure imprinting.
A range of 2-acrylamidopyridines, showing subtle differences in solution binding toward carboxylic acids, has been investigated as functional monomers in molecular imprinting. Imprinting of N-Z-L-glutamic acid with one such monomer is shown to be effective in the creation of a molecularly imprinted polymer (MIP) with recognition properties for its template and also for larger molecules containing glutamic acid residues. In comparison to a MIP prepared via a more "traditional" approach, the new polymeric receptors exhibit reduced nonspecific binding. The new receptors are compared with previously reported urea-based receptors targeting the glutamic acid residue and receptors targeting the pteridine substructure of folic acid.
Hall
Andrew J
AJ
Institut für Umweltforschung, Universität Dortmund, Otto-Hahn Strasse 6, D-44221 Dortmund, Germany.
Quaglia
Milena
M
Manesiotis
Panagiotis
P
De Lorenzi
Ersilia
E
Sellergren
Börje
B
D000178:Acrylamides / Q000737:chemistry; D001665:Binding Sites; D002264:Carboxylic Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005492:Folic Acid / Q000032:analysis* / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000737:chemistry*; D011621:Pteridines / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D014508:Urea / Q000737:chemistry; D014803:Vitamin B Complex / Q000032:analysis* / Q000737:chemistry
D000178:Acrylamides; D002264:Carboxylic Acids; D011108:Polymers; D011621:Pteridines; D011725:Pyridines; D014803:Vitamin B Complex; D018698:Glutamic Acid; D014508:Urea; D005492:Folic Acid