title: Controlling the expression ratio of two proteins by inserting a terminator between the two genes.
abstract: An approach was developed in controlling the expression ratio of two genes encoded in DNA by inserting a terminator sequence between the two genes. Escherichia coli ribosomal RNA operon T1 terminator, or E. coli tryptophane attenuater was introduced between two green fluorescent protein (GFP) mutants: ultraviolet-optimized mutant (GFPuv: F99S, M153T, V163A) and blue-shifted mutant (BFP: F64L, S65T, T145F). The ratio of the fluorescence intensity of BFP to GFPuv after transcription and translation reflected the termination efficiency of the transcriptional termination activity of the inserted terminator.
authors.affiliation: Center for International Research on MicroMechatronics, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
authors.forename: Takahiko
authors.initials: T
authors.lastname: Nojima
authors.forename: Angela C
authors.initials: AC
authors.lastname: Lin
authors.forename: Takatoki
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Isao
authors.initials: I
authors.lastname: Endo
authors.forename: Teruo
authors.initials: T
authors.lastname: Fujii
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D049408:Luminescent Agents / Q000378:metabolism; D014176:Protein Biosynthesis; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D049408:Luminescent Agents; D049452:Green Fluorescent Proteins