Proximity ligation: a specific and versatile tool for the proteomic era.
Knowledge about the total human genome sequence now provides opportunities to study its myriad gene products. However, the presence of alternative splicing, post-translational modifications, and innumerable protein-protein interactions among proteins occurring at widely different concentrations, all combine to place extreme demands on the specificity and sensitivity of assays. The choice of method also depends on matters such as whether proteins will be analyzed in body fluids and lysates, or localized inside single cells. In this review we discuss commonly used detection methods and compare these to the recently-developed proximity ligation technique.
Söderberg
Ola
O
University of Uppsala, Department of Genetics and Pathology, Rudbeck laboratory, SE-751 85 Uppsala, Sweden.
Leuchowius
Karl-Johan
KJ
Kamali-Moghaddam
Masood
M
Jarvius
Malin
M
Gustafsdottir
Sigrun
S
Schallmeiner
Edith
E
Gullberg
Mats
M
Jarvius
Jonas
J
Landegren
Ulf
U
D000818:Animals; D005818:Genetic Engineering; D006801:Humans; D040901:Proteomics / Q000379:methods*