title: On-line hyphenation of supercritical fluid extraction and two-dimensional high performance liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometer for the analysis of Ganoderma lucidum.
abstract: A novel on-line system combining supercritical fluid extraction (SFE) and two-dimensional high performance liquid chromatography (2D-HPLC) was developed. A trap column and two three-port valves were employed to couple SFE and 2D-HPLC system, which was composed of a CN column and a monolithic silica column, connected by a 10-port dual-position valve. The analytes extracted by supercritical CO2 were completely transferred to the 2D-HPLC system. After separation in two orthogonal modes, the eluents were delivered to APCI-tandem-MS for identification of the samples. In this way, sample preparation, separation, detection, and identification were integrated into an on-line system permitting analysis of the fruiting bodies of Ganoderma lucidum, and at least 73 components in the extract were resolved with calculated peak capacity of up to 1643.
authors.affiliation: National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.forename: Jicheng
authors.initials: J
authors.lastname: Duan
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liang
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
authors.forename: Yushu
authors.initials: Y
authors.lastname: Huo
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000097:Acetonitriles / Q000032:analysis; D001274:Atmospheric Pressure; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D003201:Computers; D004867:Equipment Design; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D020940:Reishi / Q000378:metabolism*; D012815:Signal Processing, Computer-Assisted; D012822:Silicon Dioxide / Q000737:chemistry; D012984:Software; D013997:Time Factors
chemical_list: D000097:Acetonitriles; D012822:Silicon Dioxide; C032159:acetonitrile