title: SNPs analysis by use of oligonucleotides containing 2'-O-methyl-2-thiouridines.
abstract: In this study, we report an improved method for the synthesis of oligonucleotides containing 2-thiouridine (s2U) derivatives. The hybridization affinity and base pair recognition ability of oligonucleotides having 2-thiouracil moieties with both the complementary RNAs and DNAs were also studied in detail. These results indicate that oligonucleotides containing s2U derivatives could be used as powerful tools for chip-based SNPs detection.
authors.affiliation: Graduate School of Life Science, Tokyo Institute of Technology.
authors.forename: Itaru
authors.initials: I
authors.lastname: Okamoto
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
mesh_terms: D020029:Base Pairing; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: C409967:2'-O-methyl-2-thiouridine; D015345:Oligonucleotide Probes; D013891:Thiouridine