title: RNA isolation using immobilized PNA.
abstract: A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.
authors.affiliation: Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohtsuki
authors.forename: Chisato
authors.initials: C
authors.lastname: Kumano
authors.forename: Taishi
authors.initials: T
authors.lastname: Manabe
authors.forename: Mizuki
authors.initials: M
authors.lastname: Kitamatsu
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sisido
mesh_terms: D001483:Base Sequence; D019921:Biotinylation; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012329:RNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D012343:RNA, Transfer / Q000302:isolation & purification*; D012356:RNA, Transfer, Leu / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D020135:Peptide Nucleic Acids; D012329:RNA, Bacterial; D012356:RNA, Transfer, Leu; D012343:RNA, Transfer