[A modified mouse model of oxygen-induced retinopathy].
OBJECTIVE
To set up a simple and effective oxygen-induced retinopathy (OIR) mouse model.


METHODS
Eyes from P17 mice in modified OIR group(Group A)and traditional OIR group (Group B) were snap frozen for immunohistochemical analysis with antibodies against GSL Flat-mounted, fluorescein-conjugated dextran-perfused retinas were examined to assess the retinal vasculature. The mortality of nursing mothers, survival rate of modeling mice and neovascularization ratio in Group A were obtained and compared with those in Group B.


RESULTS
Loss of the central retinal vasculature and the neovascular tufts were observed in GSL retinal immunohistochemical analysis. A vascular area and neovasculature area were significantly larger and more typical in Group A than those in Group B. A lower mortality of nursing mothers, higher survival rate and neovascularization ratio of modeling mice was achieved in Group A.


CONCLUSIONS
This modified oxygen-induced retinopathy mouse model is a simple, effective method, with more typical neovasculature were obtained.
Ding
Xiaoyan
X
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Liang
Xiaoling
X
Xie
Suzhen
S
Zhu
Xiaobo
X
Tang
Shibo
S
D000818:Animals; D004195:Disease Models, Animal; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000633:toxicity*; D015861:Retinal Neovascularization / Q000209:etiology*
D010100:Oxygen