[Studies of preparation, morphology and in vitro release on bio-derived material-WO-1 delivery system].
OBJECTIVE
To study the potential of a bio-derived material combined with Pluronic F-127 in vitro as a delivery vehicle for WO-1 in the bone repair therapy.


METHODS
Bio-derived materials were fabricated and loaded with WO-1 by Pluronic F-127. Micromorphology and porosity were detected by the scanning electron microscope and the digital image analysis system respectively. The WO-1 release from the system in vitro was studied by the high performance liquid chromatography.


RESULTS
Bio-derived material-WO-1 drug delivery systems were created with the interconnected pore network. The porosity and pore size of the system were 55% and 522.43 +/- 16.75 microm respectively, compared with those of bio-derived materials, which were 75% and 623. 67 +/- 12.31 microm respectively. And the main composition of the system was HA. The in vitro release kinetics of WO-1 revealed that an effective therapeutic concentration (0.2-0.8 microg/ml) of WO-1 was maintained for 6 days after a high initial burst release. Conclusion The bio-derived material-WO-1 drug delivery system can be used in the bone repair therapy. However, the in vivo study on it is still needed.
Ren
Yongxin
Y
Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, PR China.
Yang
Zhiming
Z
Xie
Huiqi
H
D000818:Animals; D018786:Bone Substitutes; D016503:Drug Delivery Systems; D013552:Swine; D023822:Tissue Engineering
D018786:Bone Substitutes