title: Expression of the Canavalia brasiliensis lectin (ConBr) in tobacco plants.
abstract: Tobacco plants were transformed with gene constructs encoding prepro-ConBr (Canavalia brasiliensis lectin). Transgenic plants confirmed by PCR expressed the recombinant protein as revealed by Western blot. However, the apparent molecular mass of the recombinant polypeptide (ca. 34 kDa) was higher than the native lectin (about 30 kDa), showing that further proteolytic processing of pro-ConBr was not detected.
authors.affiliation: Laboratório de Genética Molecular, Depto. de Biologia, Bloco 906, Campus do Pici, Universidade Federal do Ceará, Av. Humberto Monte s/n, CEP 60.455-970, Fortaleza, Ceará, Brazil.
authors.forename: Cristina Paiva
authors.initials: CP
authors.lastname: da Silveira Carvalho
authors.forename: Tuana Oliveira
authors.initials: TO
authors.lastname: Correia
authors.forename: Sandra Mara Serafim
authors.initials: SM
authors.lastname: Ribeiro
authors.forename: Ingrid Cavalcante
authors.initials: IC
authors.lastname: Silva
authors.forename: José Tadeu Abreu
authors.initials: JT
authors.lastname: de Oliveira
authors.forename: Thalles Barbosa
authors.initials: TB
authors.lastname: Grangeiro
mesh_terms: D000818:Animals; D032449:Canavalia / Q000235:genetics*; D003001:Cloning, Molecular; D018515:Plant Leaves / Q000235:genetics; D037121:Plant Lectins / Q000096:biosynthesis* / Q000235:genetics*; D030821:Plants, Genetically Modified; D011817:Rabbits; D014026:Nicotiana / Q000235:genetics*
chemical_list: D037121:Plant Lectins; C039268:lectin, Canavalia