title: [The in vitro electrical parameters of the isolated porcine ciliary epithelium].
abstract: OBJECTIVE
  To study the characteristics of ion transport across the isolated porcine ciliary epithelium to offer the good model for human ciliary epithelium study.
  
  
  METHODS
  Fresh isolated pig ciliary epithelium was mounted in a modified Ussing-type chamber to measure the electrical parameters and the effect of 0.1mM ouabain was investigated.
  
  
  RESULTS
  The potential difference (PD) across the preparations was (-0.6 +/- 0.1) mV and was consistently negative at the aqueous side. The short circuited current (SCC) and resistance (R) were found to be (-10.9 +/- 1.2) microA/cm2 and (54.6 +/- 1.6) omegacm2 respectively. Both PD and SCC were firstly hyperpolarized (stimulation) and then depolarized when 0.1 mM ouabain was applied to the aqueous side.
  
  
  CONCLUSIONS
  The polarity of transepithelial PD of the isolated porcine ciliary epithelium was negative at the aqueous side, implying an anion transport may be important in the aqueous humor formation (AHF). Na+, K+ -ATPase is located on the PE and NPE cells of the porcine ciliary epithelium and plays an important role in
authors.affiliation: Key Laboratory of Ophthalmology of the Ministry of Education and Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Yunlan
authors.initials: Y
authors.lastname: Ling
authors.forename: Chiho
authors.initials: C
authors.lastname: To
authors.forename: Mingkai
authors.initials: M
authors.lastname: Lin
authors.forename: Yehong
authors.initials: Y
authors.lastname: Zhuo
authors.forename: Fei
authors.initials: F
authors.lastname: Hou
authors.forename: Chiwing
authors.initials: C
authors.lastname: Kong
authors.forename: Jian
authors.initials: J
authors.lastname: Ge
mesh_terms: D000818:Animals; D002924:Ciliary Body / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004848:Epithelium / Q000502:physiology*; D066298:In Vitro Techniques; D017136:Ion Transport / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D010042:Ouabain / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013552:Swine
chemical_list: D004791:Enzyme Inhibitors; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase