A recombinant protein LHRH-PE40 for tumour therapy: preclinical safety studies.
LHRH-PE40, a recombinant DNA-derived protein composed of LHRH and Pseudomonas aeruginosa exotoxin A, is being developed for the treatment of malignant tumours. This experiment was designed to assess its preclinical safety. Reproductive toxicity studies, pharmacokinetic studies, single- and repeat-dose intraperitoneal or intravenous toxicity studies in mice, rats and monkeys were conducted to assess the toxicity of LHRH-PE40. In intravenous single-dose studies in mice, the LD50 was 731.26 microg/kg and 676.03 microg/kg in male and female mice respectively. In single-dose studies and repeat-dose range-finding studies in rats, dose-limited severe vascular leakage syndromes occurred. In repeat-dose long-term studies, except drug-related vascular leakage syndromes, other drug-related changes included decreased testis weight and testis atrophy. In single-dose and repeat-dose studies in monkeys, dose-limited acute tubular necrosis of the kidneys was the chief finding. In reproductive studies, drug-related changes were decreased food intakes, decreased testis weight and uterus weight, decreased foetus weight and increased foetus mortality, increased maternal and F1 offspring mortality and decreased maternal and F1 offspring body weight. Pharmacokinetic studies showed a similar half-time of distribution and clearance in mice and monkeys. Tissue distribution showed a high concentration in the kidneys and a low concentration in the brain. LHRH-PE40 induced vascular leak syndromes in rats and acute tubular necrosis in monkeys. It also led to testicle atrophy in rats and overt productive toxicity to parents and F1 generations in mice. Because of these findings, it should be monitored carefully in human clinical trials for things such as respiratory, urinary and reproductive toxicities.
Li
Jun
J
Department of Oncopathology and the Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China. xinlang168@gmail.com
Sun
Yunxia
Y
Zhang
Jinkun
J
D036002:ADP Ribose Transferases / Q000276:immunology / Q000493:pharmacokinetics / Q000633:toxicity*; D000818:Animals; D000906:Antibodies / Q000097:blood; D000970:Antineoplastic Agents / Q000276:immunology / Q000493:pharmacokinetics / Q000633:toxicity*; D001427:Bacterial Toxins / Q000276:immunology / Q000493:pharmacokinetics / Q000633:toxicity*; D004353:Drug Evaluation, Preclinical; D005098:Exotoxins / Q000276:immunology / Q000493:pharmacokinetics / Q000633:toxicity*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology / Q000493:pharmacokinetics / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008252:Macaca fascicularis; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D009369:Neoplasms / Q000188:drug therapy; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000493:pharmacokinetics / Q000633:toxicity*; D012098:Reproduction / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000254:growth & development / Q000473:pathology; D014599:Uterus / Q000187:drug effects / Q000254:growth & development; D037521:Virulence Factors / Q000276:immunology / Q000493:pharmacokinetics / Q000633:toxicity*; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000906:Antibodies; D000970:Antineoplastic Agents; D001427:Bacterial Toxins; D005098:Exotoxins; D007987:Gonadotropin-Releasing Hormone; D011993:Recombinant Fusion Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A