Synthesis and properties of novel base-discriminating fluorescent (BDF) nucleosides.
We designed a new type of pyrene-labeled base-discrimination fluorescent (BDF) nucleosides (Py)U, (Py)C, (8Py)A and (MePy)dA, which emitted strong fluorescence only when the bases opposite the BDF base are A, G, T and C, respectively. The DNA probes containing four different BDF bases enable us to distinguish single base alterations by simply mixing with a sample solution of target DNA.
Saito
Yoshio
Y
NEWCAT Institue, School of Engineering, Nihon University and SORST, Japan Science and Tecnology Agency, Tamura, Koriyama, 963-8642, Japan.
Hanawa
Kazuo
K
Hayashi
Keigo
K
Motegi
Kaori
K
Okaoto
Akimitsu
A
Saito
Isao
I
D000241:Adenosine / Q000138:chemical synthesis / Q000737:chemistry; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D009705:Nucleosides / Q000138:chemical synthesis / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D015342:DNA Probes; D005456:Fluorescent Dyes; D009705:Nucleosides; D011721:Pyrenes; C030984:pyrene; D000241:Adenosine