Selective enrichment of Ser-/Thr-phosphorylated peptides in the presence of Ser-/Thr-glycosylated peptides.
Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these conditions to achieve a beta-elimination that is highly selective for phosphorylated peptides. This is the first report of selective beta-elimination and enrichment of phosphorylated peptides in the presence of glycosylated peptides.
Poot
Alex J
AJ
Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Ruijter
Eelco
E
Nuijens
Timo
T
Dirksen
Eef H C
EH
Heck
Albert J R
AJ
Slijper
Monique
M
Rijkers
Dirk T S
DT
Liskamp
Rob M J
RM
D006020:Glycopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D010748:Phosphopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics; D012694:Serine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013912:Threonine / Q000737:chemistry
D006020:Glycopeptides; D009842:Oligopeptides; D010748:Phosphopeptides; D013912:Threonine; D012694:Serine