A novel RNA synthetic method with a 2'-O-(2-cyanoethoxymethyl) protecting group.
A novel method for the synthesis of RNA oligomers with 2-cyanoethoxymethyl (CEM) as the 2'-hydroxyl protecting group has been developed. The new method allows the synthesis of oligonucleotides with an efficiency and final purity comparable to that obtained in DNA synthesis.(1) In addition, the CEM method has the potential for application to the synthesis of very long RNA oligonucleotides.
Shiba
Yoshinobu
Y
Discovery Research Laboratories, Nippon Shinyaku Co. Ltd., 3-14-1 Sakura, Tsukuba City, Ibaraki 305-0003, Japan.
Kitagawa
Hidetoshi
H
Masutomi
Yutaka
Y
Ishiyama
Kouichi
K
Ohgi
Tadaaki
T
Yano
Junichi
J
D005019:Ethyl Ethers / Q000737:chemistry*; D008738:Methyl Ethers / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
C522071:2-cyanoethyl chloromethyl ether; D005019:Ethyl Ethers; D008738:Methyl Ethers; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D012313:RNA