Simple and sensitive determination of nisoldipine in plasma using liquid chromatography-tandem mass spectrometry.
A simple and rapid quantification method was developed for determining nisoldipine in plasma. After a simple protein precipitation with a mixture of 10% of zinc sulfate and methanol, the analytes were chromatographed on a reversed-phase C(18) column, and detected by MS/MS. The assay precision was less than 10.7%, and the accuracy ranged from 86 to 112%. The limit of quantification was 0.1 ng/ml. This method was used to measure the plasma concentration of nisoldipine from healthy subjects after a single 5-mg oral dose of nisoldipine.
Kang
Wonku
W
College of Pharmacy, Catholic University of Daegu, Kyoungbuk, South Korea. wonkuk@cu.ac.kr
D002121:Calcium Channel Blockers / Q000097:blood; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D008722:Methods; D015737:Nisoldipine / Q000008:administration & dosage / Q000097:blood*; D011506:Proteins
D002121:Calcium Channel Blockers; D011506:Proteins; D015737:Nisoldipine