title: Separation of mismatched DNA by using the affinity column immobilizing mismatch-binding ligands.
abstract: Mismatch binding ligands (MBL), ND and NA, bind to DNA containing mismatch base pairs. These MBLs were immobilized to NHS-activated affinity column via amine linker. Affinity chromatographic analyses of mismatched DNAs by using the ND-immobilized column showed clear separation of G-G and G-A from other mismatched DNAs. The results of the NA-immobilized column also showed good separation of A-A, G-A, G-G, A-C from other mismatched DNAs. The order of mobility of mismatched duplexes on MBL-immobilized column was in good agreement with that of the binding affinity in a solution phase obtained by the melting temperature measurement.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Goto
authors.forename: Akio
authors.initials: A
authors.lastname: Kobori
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Suda
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakatani
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D008024:Ligands; D009287:Naphthyridines / Q000737:chemistry; D009690:Nucleic Acid Conformation; D013997:Time Factors
chemical_list: D008024:Ligands; D009287:Naphthyridines; D004247:DNA