title: MIP3 alpha stimulates the migration of Langerhans cells in models of human papillomavirus (HPV)-associated (pre)neoplastic epithelium.
abstract: Although human papillomavirus (HPV) DNA is detected in the majority of cervical cancers and their precursors (squamous intraepithelial lesions; SIL), the persistence or progression of cervical lesions could be associated with quantitative and functional alterations of dendritic/Langerhans cells (DC/LC). As LC abnormalities have been associated with a decreased expression of macrophage inflammatory protein 3alpha (MIP3alpha) in cervical SIL, we tested the effect of exogenous MIP3alpha on the migration of LC in a (pre)neoplastic epithelium formed in vitro. By using a Boyden chamber assay, we first showed that the migratory capacity of LC generated in vitro is significantly increased in the presence of MIP3alpha compared to control medium. We next demonstrated that MIP3alpha is able to increase the 3D infiltration of LC in organotypic cultures of HPV-transformed keratinocytes. This property to stimulate LC migration was not altered after inclusion of MIP3alpha in a bioadhesive polycarbophil gel. Moreover, the function of DC to exert cytostatic effects and to present alloantigens was not altered in the presence of MIP3alpha.
authors.affiliation: Department of Pathology, CRCE, B35, University of Liege, CHU Sart Tilman, 4000 Liege, Belgium.
authors.forename: Ludivine
authors.initials: L
authors.lastname: Herman
authors.forename: Pascale
authors.initials: P
authors.lastname: Hubert
authors.forename: Jean-Hubert
authors.initials: JH
authors.lastname: Caberg
authors.forename: Brigitte
authors.initials: B
authors.lastname: Evrard
authors.forename: Witold
authors.initials: W
authors.lastname: Kedzia
authors.forename: Jacques
authors.initials: J
authors.lastname: Boniver
authors.forename: Philippe
authors.initials: P
authors.lastname: Delvenne
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D002472:Cell Transformation, Viral; D002584:Cervix Uteri; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism / Q000821:virology; D007801:Langerhans Cells / Q000378:metabolism / Q000821:virology*; D019402:Macrophage Inflammatory Proteins / Q000276:immunology / Q000378:metabolism*; D008954:Models, Biological; D009924:Organ Culture Techniques; D027383:Papillomaviridae / Q000276:immunology; D030361:Papillomavirus Infections / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D011230:Precancerous Conditions / Q000276:immunology / Q000473:pathology / Q000821:virology*
chemical_list: D019402:Macrophage Inflammatory Proteins