Laser microdissection of fungal septa as visualised by scanning electron microscopy.
Laser microdissection has been proven a successful technique to isolate single cells or groups of cells from animal and plant tissue. Here, we demonstrate that laser microdissection is suitable to isolate subcellular parts of fungal hyphae. Dolipore septa of Rhizoctonia solani containing septal pore caps were cut by laser microdissection from sections of mycelium and collected by laser pressure catapulting. Subsequently, microdissected septa were visualised using a wheat germ agglutinin labelling of cell walls, septa and septal pore caps and scanning electron microscopy. The use of laser microdissection on fungal cells opens new ways to study subcellular fungal structures and the biochemical composition of hyphal cells.
van Driel
Kenneth G A
KG
Centraalbureau voor Schimmelcultures (CBS), Royal Netherlands Academy of Arts and Sciences (KNAW), Utrecht, The Netherlands. vandriel@cbs.knaw.nl
Boekhout
Teun
T
Wösten
Han A B
HA
Verkleij
Arie J
AJ
Müller
Wally H
WH
D002469:Cell Separation / Q000295:instrumentation*; D016616:Cryoultramicrotomy / Q000295:instrumentation; D025301:Hyphae / Q000166:cytology* / Q000648:ultrastructure; D007834:Lasers; D042282:Microdissection / Q000295:instrumentation*; D008855:Microscopy, Electron, Scanning; D011312:Pressure; D012232:Rhizoctonia / Q000166:cytology* / Q000648:ultrastructure