[The animal research of recombinant adenovirus controlled by human telomerase reverse transcriptase promoter in the treatment of human prostate cancer].
OBJECTIVE
To approach the treatment efficiency of replication defective adenovirus carrying HSV-tk gene under control of the human telomerase reverse transcriptase (hTERT) promoter in a mouse xenografts model of prostate cancer.


METHODS
It was erected that the model of human prostate cancer in BALB/C nude mouse followed by locally injecting Ad-hTERT-HSV-tk and peritoneal injection of GCV. The anti-tumor efficacy was evaluated by index of tumor volume, tumor weight, relative tumor curative, and tumor growth curve. Afterwards, the TUNEL and transmission electron microscope to overview apoptosis of tumor cells were used. Finally, immunohistochemistry for detecting PCNA of tumor cells were applied.


RESULTS
Compared with the control group, all viruses treatment groups demonstrated tumor growth inhibition. Among 3 treatment groups, antitumoral effect of Ad-hTERT-HSV-tk/GCV was much stronger than other two groups (P < 0.05). Obvious necrosis and apoptosis was observed by TUNEL, and PCNA was detected by immunohistochemistry in tumor tissues in all viruses treatment group.


CONCLUSIONS
Ad-hTERT-HSV-tk/GCV system is highly efficacious to inhabit the growth of human prostate cancer.
Zhang
Yong
Y
Department of Urology, Second Hospital, Hebei Medical University, Shijiazhuang 050000, China.
Qi
Jin-chun
JC
Lian
Wen-feng
WF
Cai
Wen-qing
WQ
Li
Wei
W
Liu
Kai-long
KL
D000256:Adenoviridae / Q000201:enzymology / Q000235:genetics*; D000818:Animals; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000473:pathology / Q000628:therapy*; D011995:Recombination, Genetic; D019098:Telomerase / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics
D013937:Thymidine Kinase; C509186:TERT protein, human; D019098:Telomerase