YCA1 participates in the acetic acid induced yeast programmed cell death also in a manner unrelated to its caspase-like activity.
Yeast cells lacking the metacaspase-encoding gene YCA1 (Deltayca1) were compared with wild-type (WT) cells with respect to the occurrence, nature and time course of acetic-acid triggered death. We show that Deltayca1 cells undergo programmed cell death (PCD) with a rate lower than that of the WT and that PCD in WT cells is caused at least in part by the caspase activity of Yca1p. Since in Deltayca1 cells this effect is lost, but z-VAD-fmk does not prevent both WT and Deltayca1 cell death, PCD in WT cells occurs via a Yca1p caspase and a non-caspase route with similar characteristics.
Guaragnella
Nicoletta
N
CNR, Istituto di Biomembrane e Bioenergetica, I-70126 Bari, Italy.
Pereira
Clara
C
Sousa
Maria João
MJ
Antonacci
Lucia
L
Passarella
Salvatore
S
Côrte-Real
Manuela
M
Marra
Ersilia
E
Giannattasio
Sergio
S
D019342:Acetic Acid / Q000494:pharmacology*; D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D000590:Amino Acid Chloromethyl Ketones; D029701:Saccharomyces cerevisiae Proteins; C476093:z-Val-Ala-Asp(Ome)-fluoromethylketone; D020169:Caspases; C456272:MCA1 protein, S cerevisiae; D019342:Acetic Acid