Synthesis and properties of 2'-O-methyl-4'-thioRNA.
In this presentation, we will discuss the synthesis and properties of 2'-O-methyl-4'-thioRNA, an RNA molecule consisting of 2'-O-methyl-4'-thionucleosides. We first synthesized 2'-O-methyl-4'-thiouridine and -cytidine derivatives via 2,2'-O-anhydro-4'-thiouridine. The RNA consisting of 2'-O-methyl-4'-thiopyrimidine nucleosides and 2'-O-methylpurine nucleosides, 2'-OMe-4'-thioRNA, was synthesized on a DNA synthesizer according to the standard phosphoramidite protocol.
Takahashi
Mayumi
M
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Inoue
Naonori
N
Minakawa
Noriaki
N
Matsuda
Akira
A
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry*; D013696:Temperature; D013872:Thionucleosides / Q000138:chemical synthesis / Q000737:chemistry*; D013891:Thiouridine / Q000737:chemistry
D013872:Thionucleosides; D013891:Thiouridine; D012313:RNA; D004247:DNA