title: Characterization of DNA hybridization on partially aminated diamond by aromatic compounds.
abstract: Here, we report a novel method of micropatterning oligonucleotides via aromatic groups as linkers on partially amino-terminated diamond and the inherence on subsequent hybridization. The covalent immobilization of probe oligonucleotides and characterization of immobilized probe oligonucleotides with carboxylic compounds were investigated by X-ray photoelectron spectroscopy (XPS). To confirm the effects of linker flexibility in a low amino group on diamond for probe oligonucleotides, three kinds of dicarboxylic compound--adipic acid, terephthalic acid, and trimesic acid--were used for immobilization of probe oligonucleotides, like linkers; and these oligonucleotides were hybridized with target oligonucleotides labeled with Cy 5 on the micropatterned diamond surface. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.
authors.affiliation: School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yang
authors.forename: Kwang-Soup
authors.initials: KS
authors.lastname: Song
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Zhang
authors.forename: Munenori
authors.initials: M
authors.lastname: Degawa
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Iwao
authors.initials: I
authors.lastname: Ohdomari
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawarada
mesh_terms: D002232:Carbocyanines / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D018130:Diamond / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013052:Spectrometry, X-Ray Emission / Q000379:methods
chemical_list: D002232:Carbocyanines; D003432:Cross-Linking Reagents; D009841:Oligonucleotides; C085321:cyanine dye 5; D018130:Diamond