title: Rapid determination of protopine, allocryptopine, sanguinarine and chelerythrine in fruits of Macleaya cordata by microwave-assisted solvent extraction and HPLC-ESI/MS.
abstract: An isocratic high-performance liquid chromatographic method coupled with electrospray mass spectrometry was developed to determine protopine, allocryptopine, sanguinarine and chelerythrine in fruits of Macleaya cordata. The sample was extracted with hydrochloric acid aqueous solution using microwave-assisted extraction method. The extracts were separated on a C8 reversed-phase HPLC column with acetonitrile:acetate buffer as mobile phase, and full elution of all analytes was realized isocratically within 10 min. The abundance of pseudomolecule ions was recorded using selected ion recording at m/z 354.4, 370.1, 332.5, 348.5 and 338.5 for protopine, allocryptopine, sanguinarine, chelerythrine and the internal standard, jatrorrhizine, respectively. Internal standard curves were used for the quantification of protopine, allocryptopine, sanguinarine and chelerythrine, which showed a linear range of 0.745-74.5, 0.610-61.0, 0.525-105 and 0.375-75 microg/mL, respectively, with correlation coefficients of 0.9995, 0.9992, 0.9993 and 0.9989, and limits of detection of 3.73, 3.05, 1.60 and 1.11 ng/mL, respectively.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, People's Republic of China.
authors.forename: Xu-Biao
authors.initials: XB
authors.lastname: Luo
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Shou-Zhuo
authors.initials: SZ
authors.lastname: Yao
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D053119:Benzophenanthridines / Q000737:chemistry*; D001600:Berberine Alkaloids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D007546:Isoquinolines / Q000737:chemistry*; D008872:Microwaves; D015394:Molecular Structure; D028101:Papaveraceae / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D000470:Alkaloids; D053119:Benzophenanthridines; D001600:Berberine Alkaloids; D007546:Isoquinolines; C005705:sanguinarine; C016299:chelerythrine; C109505:allocryptopine; C009093:protopine