[Design of a microfluidic immunoassay system based on superparamagnetic microbeads].
We use direct-write laser micromachining technology to fabricate the microfluidic chip, and to establish a microfluidic chemiluminescence immunoassay system based on superparamagnetic microbeads, for detecting alpha- fetoprotein (AFP). The AFP analysis can be completed in 20 minutes with 5 microl sample and 5 microl reagent, and there is a good linear correlation in the range of 1-800 ng/ml.
Huang
Hui
H
Key Lab of Biomechanics and Tissue Engineering, State Education Ministry, Bioenginering College, Chongqing University, Chongqing.
Zheng
Xiao-Lin
XL
Pan
Jing
J
Zhao
Zhi-Qiang
ZQ
Pu
Xiao-Yun
XY
D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D000509:alpha-Fetoproteins / Q000032:analysis
D000509:alpha-Fetoproteins