title: Human embryonic stem cells.
abstract: Human embryonic stem cells hold great promise in furthering our treatment of disease and increasing our understanding of early development. This chapter describes protocols for the derivation and maintenance of human embryonic stem cells. In addition, it summarizes briefly several alternative methods for the culture of human embryonic stem cells. Thus, this chapter provides a good starting point for researchers interested in harnessing the potential of human embryonic stem cells.
authors.affiliation: Harvard University, Howard Huges Medical Institute, Cambridge, Massachusetts, USA.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Akutsu
authors.forename: Chad A
authors.initials: CA
authors.lastname: Cowan
authors.forename: Douglas
authors.initials: D
authors.lastname: Melton
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D017364:Collagenases; D003470:Culture Media; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006801:Humans; D051379:Mice; D014357:Trypsin
chemical_list: D003470:Culture Media; D014357:Trypsin; D017364:Collagenases