Etodolac, a selective cyclooxygenase-2 inhibitor, induces apoptosis by activating caspases in human malignant rhabdoid tumor cells (FRTK-1).
Malignant rhabdoid tumor (MRT) is a rare and highly aggressive tumor presenting in the kidney and soft tissue in childhood. However, effective treatment for MRT has not been established. We investigated the antitumor effect of etodolac, a selective cyclooxygenase-2 inhibitor, on MRT cells in vitro using the MRT cell line FRTK-1. Etodolac induced apoptosis of FRTK-1 cells through activation of caspase-8, -9 and -3. Moreover, several caspase inhibitors completely or partially inhibited etodolac-induced apoptosis. Our data indicated that etodolac had an antitumor effect on MRT cells and holds promise as a novel therapeutic strategy for MRT.
Hakozaki
Michiyuki
M
First Department of Pathology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan. paco@fmu.ac.jp
Hojo
Hiroshi
H
Kikuchi
Shinichi
S
Abe
Masafumi
M
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D020169:Caspases / Q000378:metabolism*; D045744:Cell Line, Tumor; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D017308:Etodolac / Q000494:pharmacology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D018335:Rhabdoid Tumor / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology
D000590:Amino Acid Chloromethyl Ketones; D052246:Cyclooxygenase 2 Inhibitors; D015853:Cysteine Proteinase Inhibitors; D007527:Isoenzymes; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; D017308:Etodolac; D020169:Caspases