Characterization of Salmonella enterica serovar Typhimurium DT104 invasion in an epithelial cell line (IPEC J2) from porcine small intestine.
Salmonella Typhimurium DT104 is an emerging enteric pathogen in swine of increasing medical importance. In this study, the time course and the actin-dependent host signaling processes necessary for invasion of a S. Typhimurium DT104 field isolate were investigated in IPEC J2 epithelial cells derived from porcine small intestine. Internalized bacteria were quantified by a gentamicin resistance assay. DT104 internalization into epithelial monolayers increased steadily between 15 and 120min after apical inoculation. Internalization was reduced by the Rho GTPase inhibitor mevastatin, the N-WASP inhibitor wiskostatin and the actin-disrupting agent cytochalasin D, but not the Rac1 GTPase inhibitor NSC-23766. Early DT104 invasion of porcine enterocytes appears to be mediated by Rac1 GTPase-independent changes in epithelial actin assembly.
Brown
David R
DR
Department of Veterinary and Biomedical Sciences, University of Minnesota, St Paul, MN 55108-6010, USA. brown013@umn.edu
Price
Lisa D
LD
D000818:Animals; D002227:Carbazoles / Q000494:pharmacology; D002460:Cell Line; D015169:Colony Count, Microbial; D015638:Cytochalasin D / Q000494:pharmacology; D017097:Electric Impedance; D004847:Epithelial Cells / Q000382:microbiology; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology; D007421:Intestine, Small / Q000166:cytology / Q000382:microbiology*; D008148:Lovastatin / Q000031:analogs & derivatives / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011412:Propanolamines / Q000494:pharmacology; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000502:physiology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D013997:Time Factors
D002227:Carbazoles; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; D011412:Propanolamines; C489942:wiskostatin; C012258:mevastatin; D015638:Cytochalasin D; D008148:Lovastatin