Measurement of circular dichroism and structural chemical research of d(CG)6 and d(TA)6.
It was known that d(CG)(6) became left-handed Z-DNA by a theoretical calculation. We carried out a CD measurement of d(CG)(6) at room temperature. B-Z transition was occurred when I raised the concentration of Mg salt. In addition, I confirmed that the Mg salt concentration caused B-Z transition by the lower concentration under the existence of cobalt hexamine. Structure of d(TA)(6) is not known. We performed the CD measurement of d(TA)(6) at low temperature. Structure changed from B type when I raised the Mg salt concentration in an existence of cobalt hexamine. I am examining structure now.
Tozuka
Yoshitaka
Y
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Yoshikawa
Yuki
Y
Ohishi
Hirofumi
H
Kobayashi
Yuji
Y
Da-Yang
Zhou
Z
Nakatani
Kazuhiko
K
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D043542:DNA, Z-Form / Q000737:chemistry*; D015636:Magnesium Chloride / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
D043542:DNA, Z-Form; D009838:Oligodeoxyribonucleotides; D015636:Magnesium Chloride; D004247:DNA