C8orf4/TC-1 (thyroid cancer-1) gene expression in thyroid cancer and goiter.
BACKGROUND
The expression of the thyroid cancer-1(TC-1) gene seems to be related with malignant transformation in the thyroid tissue.


OBJECTIVE
We evaluated the potential use of TC-1 gene expression as a marker of malignancy in thyroid nodules.


METHODS
A total of 92 frozen thyroid samples were studied, including 46 samples from thyroid nodules (19 papillary carcinomas, 1 follicular carcinoma, 24 adenomatous goiters, and 2 follicular adenomas) and 46 samples from normal surrounding thyroid tissue. Total RNA was extracted and TC-1 expression was assessed by semiquantitative Multiplex PCR. Results were verified using real-time RT-PCR in some of the samples.


RESULTS
Overall mean TC-1 gene expression (normalized by the ABL gene) was 1.73 +/- 1.67 (0.33-9.33). There was a significant difference (p < 0.001) between TC-1 gene expression in benign thyroid lesions (1.07 +/- 0.10) and carcinomas (2.73 +/- 0.51).


CONCLUSIONS
Our results suggest that TC-1 gene expression may be useful in the differential diagnosis of goiters and thyroid papillary carcinomas.
de Melo Martins
Poliana Cristina
PC
Laboratory of Molecular Genetics, São Paulo, Brazil.
Parise Junior
Orlando
O
Pereira Hors
Cora
C
Villela Miguel
Roberto Elias
RE
da Costa Andrade
Valéria Cristina
VC
Garicochea
Bernardo
B
D000236:Adenoma / Q000000981:diagnostic imaging / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000368:Aged; D002291:Carcinoma, Papillary / Q000000981:diagnostic imaging / Q000235:genetics; D016373:DNA, Antisense / Q000235:genetics; D005260:Female; D006042:Goiter / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013964:Thyroid Neoplasms / Q000000981:diagnostic imaging / Q000235:genetics*; D014057:Tomography, X-Ray Computed
D016373:DNA, Antisense; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C418741:TCIM protein, human