title: Analysis of PPAR alpha function in human kidney cell line using siRNA.
abstract: The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. PPARalpha is mainly expressed in the liver, kidney, heart and muscle. PPARalpha activates fatty acid catabolism, stimulates gluconeogenesis and ketone body synthesis and is involved in the control of lipoprotein assembly. Although PPARalpha is well characterized in the liver, its physiological function is unknown in the kidney. To investigate the intimate function of PPARalpha in the kidney, we analyzed the target gene expression in human metastatic renal cell carcinoma cell line, Caki-1, using small interfering RNA (siRNA) against PPARalpha and real-time RT-PCR methods. We found that some selected genes (long-chain fatty-acid-CoA ligase (FACL1), carnitine palmitoyltransferase 1A (CPT1A), adipose differentiation-related protein (ADRP) and aquaporin 3 (AQP3)) were down-regulated by PPARalpha siRNA. These results suggest that PPARalpha regulates fatty acid metabolism and body water homeostasis in this cell line.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Tachibana
authors.forename: Naohiko
authors.initials: N
authors.lastname: Anzai
authors.forename: Chihiro
authors.initials: C
authors.lastname: Ueda
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Katayama
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Kirino
authors.forename: Rika
authors.initials: R
authors.lastname: Takahashi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamasaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishimoto
authors.forename: Toshiya
authors.initials: T
authors.lastname: Tanaka
authors.forename: Takao
authors.initials: T
authors.lastname: Hamakubo
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Ueda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Arai
authors.forename: Juro
authors.initials: J
authors.lastname: Sakai
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kodama
authors.forename: Takefumi
authors.initials: T
authors.lastname: Doi
mesh_terms: D001834:Body Water / Q000378:metabolism; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D047493:PPAR alpha / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D034741:RNA, Small Interfering
chemical_list: D005227:Fatty Acids; D047493:PPAR alpha; D034741:RNA, Small Interfering