Inhibiting transcription of chromosomal DNA using antigene RNAs.
Transcription start sites are critical switches for converting recognition of chromosomal DNA into synthesis of RNA. We report that antigene peptide nucleic acid (agPNA) oligomers or antigene RNA (agRNA) duplexes can target transcription start sites and block gene expression. Our data suggest that chromosomal DNA within cells is readily accessible to recognition by synthetic agents. agPNAs and agRNAs will be useful tools for silencing gene expression and will provide insights into natural mechanisms for controlling transcription.
Janowski
Bethany A
BA
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9041, USA.
Corey
David R
DR
D002460:Cell Line; D033481:Chromosomes, Mammalian; D004247:DNA / Q000737:chemistry; D020868:Gene Silencing; D006801:Humans; D020135:Peptide Nucleic Acids / Q000494:pharmacology*; D012330:RNA, Double-Stranded / Q000494:pharmacology*; D011980:Receptors, Progesterone / Q000235:genetics; D024363:Transcription Initiation Site; D014158:Transcription, Genetic / Q000187:drug effects
D020135:Peptide Nucleic Acids; D012330:RNA, Double-Stranded; D011980:Receptors, Progesterone; D004247:DNA