Metaproteomics approach to study the functionality of the microbiota in the human infant gastrointestinal tract.
A metaproteomics approach comprising two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization-time of flight (mass spectrometry) was applied to the largely uncultured infant fecal microbiota for the first time. The fecal microbial metaproteome profiles changed over time, and one protein spot contained a peptide sequence that showed high similarity to those of bifidobacterial transaldolases.
Klaassens
Eline S
ES
Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands. Eline.klaassens@wur.nl
de Vos
Willem M
WM
Vaughan
Elaine E
EE
D015180:Electrophoresis, Gel, Two-Dimensional; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010455:Peptides / Q000502:physiology; D020543:Proteome / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014153:Transaldolase / Q000378:metabolism
D010455:Peptides; D020543:Proteome; D014153:Transaldolase