Attachment of motile bacterial cells to prealigned holed microarrays.
Construction of biomotors is an exciting area of scientific research that holds great promise for the development of new technologies with broad potential applications in areas such as the energy industry and medicine. Herein, we demonstrate the fabrication of prealigned microarrays of motile Escherichia coli bacterial cells on SiOx substrates. To prepare these arrays, holed surfaces with a gold layer on the bottom of the holes were utilized. The attachment of bacteria to the holes was achieved via nonspecific interactions using poly-l-lysine hydrobromide (PLL). Our data suggest that a single motile bacterial cell can be selectively attached to an individual hole on a surface and bacterial cell binding can be controlled by altering the pH, with the greatest occupancy occurring at pH 7.8. Cells attached to hole arrays remained motile for at least 4 h. These data indicate that holed surface structures provide a promising footprint for the attachment of motile bacterial cells to form high-density site-specific functional bacterial microarrays.
Rozhok
Sergey
S
The Department of Chemistry, Loyola University-Chicago, 1068 N. Sheridan Road, Chicago, IL 60626, USA.
Fan
Zhifang
Z
Nyamjav
Dorjderem
D
Liu
Chang
C
Mirkin
Chad A
CA
Holz
Richard C
RC
D018914:Cells, Immobilized / Q000737:chemistry / Q000166:cytology; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology*; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D046228:Microarray Analysis / Q000379:methods; D011107:Polylysine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
D011107:Polylysine; D006046:Gold; D012822:Silicon Dioxide