title: A pepsin-resistant 20 kDa protein found in red kidney bean (Phaseolus vulgaris L.) identified as basic subunit of legumin.
abstract: The digestibility of proteins in red kidney bean (Phaseolus vulgaris L.) was examined by in vitro pepsin assay. A 20-kDa polypeptide that remained highly stable after heat processing was identified as a basic subunit of legumin. The results of a monobromobimane (mBBr) labeling test implied that this protein contained rigid intramolecular disulfide bonds, which might contribute to pepsin resistance.
authors.affiliation: National Food Research Institute, Tsukuba, Ibaraki. michiko@affrc.go.jp
authors.forename: Michiko
authors.initials: M
authors.lastname: Momma
mesh_terms: D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010434:Pepsin A / Q000737:chemistry*; D027805:Phaseolus / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D000097793:Legumins
chemical_list: D010434:Pepsin A; D010940:Plant Proteins; D000097793:Legumins