Suppression of HCV RNA replication by baculovirus-mediated shRNA expression.
We describe a HCV core-short-hairpin RNA (shRNA) expressed from a human U6 Pol II promoter, which synergistically enhanced the inhibition of HCV replication in HCV replicon. We also describe the construction of recombinant baculovirus expressing shRNAs of the HCV core gene under the control of the U6 promoter. The anti-HCV shRNAs may provide a practical basis for applying shRNA-based gene therapy to the treatment of HCV.
Suzuki
Hitoshi
H
Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.
Kaneko
Hiroyasu
H
Tamai
Nobushige
N
Miyano-Kurosaki
Naoko
N
Hashimoto
Kahoko
K
Shimotohno
Kunitada
K
Takaku
Hiroshi
H
D016367:Baculoviridae / Q000235:genetics*; D005822:Genetic Vectors; D016174:Hepacivirus / Q000235:genetics* / Q000502:physiology; D034622:RNA Interference; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D014758:Viral Core Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D014779:Virus Replication
D022661:RNA, Untranslated; D012367:RNA, Viral; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus