title: AFM fishing nanotechnology is the way to reverse the Avogadro number in proteomics.
abstract: Future development of proteomics may be hindered by limitations in the concentration sensitivity of widespread technological approaches. The concentration sensitivity limit (CSL) of currently used approaches, like 2-DE/LC separation coupled with MS detection, etc., varies from 10(-9) to 10(-12) M. Therefore, proteomic technologies enable detection of up to 20% of the protein species present in the plasma. New technologies, like atomic force microscopy (AFM molecular detector), enable the counting of single molecules, whereas biospecific fishing can be used to capture these molecules from the biomaterial. At the same time, fishing also has thermodynamic limitations due to the reversibility of the binding. In cases where the fishing becomes irreversible, its combination with an AFM detector enables the registration of single protein molecules, and that opens up a way to lower the CSL down to the reverse Avogadro number.
authors.affiliation: Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Pogodinskaya Street, Moscow, Russia. archak@ibmc.msk.ru
authors.forename: Alexander Ivanovich
authors.initials: AI
authors.lastname: Archakov
authors.forename: Yurii Dmitrievich
authors.initials: YD
authors.lastname: Ivanov
authors.forename: Andrey Valerevich
authors.initials: AV
authors.lastname: Lisitsa
authors.forename: Victor Gavrilovich
authors.initials: VG
authors.lastname: Zgoda
mesh_terms: D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology; D040901:Proteomics; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization