Distribution of low molecular weight platelet aggregation inhibitors from snake venoms.
An assay of platelet aggregation inhibitors measured by the turbidimeter using Aggregometer PAM 8C (Mebanix) was performed after each crude snake venom (57 species) was subjected to ultrafiltration using MILLIPORE UFP 1 LGC. The snake venoms of Viperidae (three species), Elapidae (11 species), and Hydrophiidae (two species) inhibited ADP-induced rabbit platelet aggregation. In particular, six venoms of Bitis gabonica, Pseudocerastes persicus, Dendroaspis angusticeps, D. polylepis, Ophiophagus hannah, and N. nigricollis crawshawii strongly inhibited platelet aggregation. Furthermore, adenosine was identified from Bitis gabonica venom using HPLC and FAB/MS analysis.
Oyama
Etsuko
E
Department of Hygenic Chemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
Takahashi
Hidenobu
H
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004546:Elapid Venoms / Q000737:chemistry / Q000494:pharmacology; D017815:Elapidae / Q000502:physiology; D008970:Molecular Weight; D009391:Nephelometry and Turbidimetry; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D011817:Rabbits; D012910:Snake Venoms / Q000737:chemistry* / Q000494:pharmacology; D012911:Snakes / Q000502:physiology*; D014757:Viper Venoms / Q000737:chemistry / Q000494:pharmacology; D017819:Viperidae / Q000502:physiology
D004546:Elapid Venoms; D010975:Platelet Aggregation Inhibitors; D012910:Snake Venoms; D014757:Viper Venoms