title: Msn2p/Msn4p-activation is essential for the recovery from freezing stress in yeast.
abstract: Since it seems quite difficult for frozen cells to repair the damage caused by freezing, the adequate responses appear to be induced during and/or after the thawing process to recover from the damage due to freezing. In this study, the cellular events happening upon the return from freezing at -30 degrees C to a growth temperature (28 degrees C) were investigated. Yap1p, an oxidative stress-responsive transcription factor, was not activated in the thawed cells, indicating that no serious oxidative stress was generated in the frozen-thawed cells. On the other hand, Msn2p and Msn4p, general stress-responsive transcription factors, were activated in the thawed cells and caused the increased expression of a number of Msn2p/Msn4p-target genes including SOD1, SOD2, and several HSP genes. Since almost no expression of Msn2p/Msn4p-target genes was induced before thawing, these results indicate that Msn2p and Msn4p play a role during the recovery process from freezing.
authors.affiliation: Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan. izawa@kais.kyoto-u.ac.jp
authors.forename: Shingo
authors.initials: S
authors.lastname: Izawa
authors.forename: Kayo
authors.initials: K
authors.lastname: Ikeda
authors.forename: Takumi
authors.initials: T
authors.lastname: Ohdate
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Inoue
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D017209:Apoptosis / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005615:Freezing; D018869:Heat-Shock Response / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C081935:MSN2 protein, S cerevisiae; C081937:MSN4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors