title: Detection of novel papillomaviruslike sequences in paraffin-embedded specimens of invasive and in situ squamous cell carcinomas from cats.
abstract: OBJECTIVE
  To detect and partially characterize papillomavirus (PV) DNA in squamous cell carcinoma (SCC) tumor specimens from cats.
  
  
  METHODS
  54 formalin-fixed paraffinembedded skin biopsy specimens were examined. Specimens originated from Bowenoid in situ SCC (BISC; n = 21), invasive SCC (22), and skin affected by miscellaneous nonneoplastic conditions (11).
  
  
  METHODS
  Samples from each tissue block underwent DNA extraction after deparaffinization, and PCR assays were performed. Two sets of primers derived from PV E1 were used. The first set of primers was designed for the narrow-range PCR assay and was able to generate amplification products of feline PV (FePV), canine oral PV, or closely related PVs. The second set of primers was selected for the broad-range PCR assay because of its ability to amplify DNA from 64 human PVs. Sequence analysis of each amplified DNA was performed.
  
  
  RESULTS
  1 of the 21 specimens of BISC was positive for PV DNA on the basis of narrow-range PCR assay results, whereas all the other specimens (BISC, invasive SCC, and controls) had negative results for PV DNA. In contrast, 5 of 21 BISC specimens and 4 of 22 invasive SCC specimens were positive for PV DNA on the basis of broad-range PCR assay results. Sequence analysis revealed that only 1 specimen was infected by a virus closely related to classic FePV. In the 8 other specimens positive for PV DNA, DNA of unknown PVs was uncovered.
  
  
  CONCLUSIONS
  Bowenoid in situ SCC and invasive SCC of cats may be associated with PVs of genetic diversity.
authors.affiliation: Clinic for Small Animal Internal Medicine, Dermatology Unit, Vetsuisse Faculty, University of Zürich, Winterthurerstrasse 260, CH 8057 Zurich, Switzerland.
authors.forename: Gilles
authors.initials: G
authors.lastname: Nespeca
authors.forename: Paula
authors.initials: P
authors.lastname: Grest
authors.forename: Wayne S
authors.initials: WS
authors.lastname: Rosenkrantz
authors.forename: Mathias
authors.initials: M
authors.lastname: Ackermann
authors.forename: Claude
authors.initials: C
authors.lastname: Favrot
mesh_terms: D000818:Animals; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D002371:Cat Diseases / Q000473:pathology / Q000821:virology*; D002415:Cats; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016612:Paraffin Embedding / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012878:Skin Neoplasms / Q000473:pathology / Q000662:veterinary* / Q000821:virology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral