Relationships of Drosophila melanogaster RECQ5/QE to cell-cycle progression and DNA damage.
Members of the RecQ family of DNA helicases are involved in the cellular response to DNA damage and are regulated in the cell-cycle. However, little is known about RecQ5, one of these members. The level of RECQ5/QE, Drosophila melanogaster RecQ5, was increased after the exposure of cultured cells to methyl-methanesulfonate. Transgenic flies that overexpressed RECQ5/QE in their developing eye primordia showed mild roughening of the ommatidial lattice. DNA-damaging agents and the mei-41 mutation enhanced the phenotype caused by RECQ5/QE overexpression. Overexpression of RECQ5/QE perturbed the progression of the cell-cycle in response to DNA damage in the eye imaginal discs. These results suggest that RECQ5/QE interacts with components of the cell-cycle during its progression in response to DNA damage.
Nakayama
Minoru
M
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka 573-0101, Japan.
Maruyama
Sayako
S
Kanda
Hiroshi
H
Ohkita
Noriko
N
Nakano
Katsunori
K
Ito
Fumiaki
F
Kawasaki
Katsumi
K
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D004249:DNA Damage / Q000235:genetics*; D004331:Drosophila melanogaster / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*; D005123:Eye / Q000166:cytology / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D008855:Microscopy, Electron, Scanning; D010641:Phenotype; D053484:RecQ Helicases / Q000235:genetics / Q000378:metabolism*
D053484:RecQ Helicases