Monoclonal antibodies to phosphatidylinositol phosphate neutralize human immunodeficiency virus type 1: role of phosphate-binding subsites.
Both a murine monoclonal antibody to phosphatidylinositol phosphate (PIP) and a human monoclonal antibody (4E10) that is known to have broadly neutralizing capabilities against primary isolates of human immunodeficiency virus type 1 (HIV-1) bound to PIP, as determined by enzyme-linked immunosorbent assay. Each of the antibodies had antigen subsite binding specificities in aqueous medium for small phosphate-containing molecules and for inositol. The anti-PIP monoclonal antibody inhibited infection by two HIV-1 primary isolates in neutralization assays employing primary human peripheral blood mononuclear cells. The data suggest that PIP or related lipids having free phosphates could serve as targets for the neutralization of HIV-1.
Brown
Bruce K
BK
Henry M. Jackson Foundation, 13 Taft Court, Rockville, Maryland 20850, USA.
Karasavvas
Nicos
N
Beck
Zoltan
Z
Matyas
Gary R
GR
Birx
Deborah L
DL
Polonis
Victoria R
VR
Alving
Carl R
CR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity; D002478:Cells, Cultured; D003429:Cross Reactions; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007294:Inositol / Q000378:metabolism; D007963:Leukocytes, Mononuclear; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D051379:Mice; D009500:Neutralization Tests; D010710:Phosphates / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates / Q000276:immunology*
D000911:Antibodies, Monoclonal; D008055:Lipids; D010710:Phosphates; D018129:Phosphatidylinositol Phosphates; D007294:Inositol