title: Changes in allergenicity and digestibility of squid tropomyosin during the Maillard reaction with ribose.
abstract: The effect of the Maillard reaction on the allergenicity of squid tropomyosin (TM) was investigated. When TM was reacted with ribose (TM-ribose), its human-specific IgE-binding ability decreased markedly and alpha-chymotryptic digestibility of TM was also altered at the early stage of the Maillard reaction. On the other hand, the modification of the lysine residues in TM using 2,4,6-trinitrobenzenesulfonic acid had no effect on the allergenicity and alpha-chymotryptic digestibility of TM. Therefore, the structural change in TM induced by the Maillard reaction would cause the reduction of the allergenicity, rather than the block of lysine residues. Although peptic digestion diminished the specific IgE-binding ability of TM, the reduction of the allergenicity by the Maillard reaction remained after peptic digestion. These results suggest that hypersensitive reaction of TM-ribose in the human body might be lower than that of native TM.
authors.affiliation: Graduate School of Fisheries Sciences, Hokkaido University, Minato 3-1-1, Hakodate, Hokkaido, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakamura
authors.forename: Fumi
authors.initials: F
authors.lastname: Sasaki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Watanabe
authors.forename: Takao
authors.initials: T
authors.lastname: Ojima
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Ahn
authors.forename: Hiroki
authors.initials: H
authors.lastname: Saeki
mesh_terms: D000485:Allergens / Q000032:analysis*; D000818:Animals; D002918:Chymotrypsin / Q000378:metabolism; D049832:Decapodiformes / Q000737:chemistry*; D004063:Digestion; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008239:Lysine / Q000737:chemistry; D015416:Maillard Reaction; D010434:Pepsin A / Q000378:metabolism; D012266:Ribose / Q000737:chemistry; D014335:Tropomyosin / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000485:Allergens; D014335:Tropomyosin; D007073:Immunoglobulin E; D012266:Ribose; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; D010434:Pepsin A; D008239:Lysine