In vitro selection of RNA aptamers for the Escherichia coli release factor 1.
We carried out an in vitro selection of RNA aptamers that bind to Escherichia coli release factor 1 (E. coli RF1). The selected aptamer (class II) showed an apparent dissociation constant of nM range. The binding of the class II aptamer with E. coli RF1 is highly specific (orthogonal), allowing selective inhibition of RF1 activity in the E. coli translation system.
Ogawa
Atsushi
A
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Nishi
Teruyuki
T
Sando
Shinsuke
S
Aoyama
Yasuhiro
Y
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D029968:Escherichia coli Proteins / Q000037:antagonists & inhibitors*; D008969:Molecular Sequence Data; D010454:Peptide Termination Factors / Q000037:antagonists & inhibitors*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry; D012356:RNA, Transfer, Leu / Q000737:chemistry; D052156:SELEX Aptamer Technique; D013698:Templates, Genetic
D052157:Aptamers, Nucleotide; D029968:Escherichia coli Proteins; D010454:Peptide Termination Factors; D012333:RNA, Messenger; D012356:RNA, Transfer, Leu; C489005:prfA protein, E coli