title: Conferring cadmium resistance to mature tobacco plants through metal-adsorbing particles of tomato mosaic virus vector.
abstract: Tomato mosaic virus vectors were designed that produced, by a translational readthrough, a fusion protein consisting of coat protein and metal-binding peptide, as a result of which particles were expected to present the metal-binding peptides on their surface. When inoculated in plants, they were expected to replicate and form a metal-adsorbing artificial sink in the cytoplasm, so as to reduce metal toxicity. Vectors were constructed harbouring sequences encoding various lengths of polyhistidine as a metal-binding peptide. One of the vectors, TLRT6His, which contains a 6 x histidine sequence, moved systemically in tobacco plants, and its particles were shown to retain cadmium ions by an in vitro assay. When a toxic amount of cadmium was applied, the toxic effect was much reduced in TLRT6His-inoculated tobacco plants, probably as a result of cadmium adsorption by TLRT6His particles in the cytosol. This shows the possible use of an artificial sink for metal tolerance and the advantage of employing a plant viral vector for phytoremediation.
authors.affiliation: Environmental Plant Research Group, RIKEN Plant Science Center, Suehiro-cho 1-7-22, Tsurumi, Yokohama, 230-0045, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Shingu
authors.forename: Satoko
authors.initials: S
authors.lastname: Yokomizo
authors.forename: Makoto
authors.initials: M
authors.lastname: Kimura
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Ono
authors.forename: Isamu
authors.initials: I
authors.lastname: Yamaguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hamamoto
mesh_terms: D002104:Cadmium / Q000378:metabolism*; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism / Q000502:physiology; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D017898:Tobamovirus / Q000235:genetics* / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D002104:Cadmium