Pluripotent stem cells from germ cells.
To date, stem cells have been derived from three sources of germ cells. These include embryonic germ cells (EGCs), embryonal carcinoma cells (ECCs), and multipotent germ line stem cells (GSCs). EGCs are derived from primordial germ cells that arise in the late embryonic and early fetal period of development. ECCs are derived from adult testicular tumors whereas GSCs have been derived by culturing spermatogonial stem cells from mouse neonates and adults. For each of these lines, their pluripotency has been demonstrated by their ability to differentiate into cell types derived from the three germ layers in vitro and in vivo and in chimeric animals, including germ line transmission. These germ line-derived stem cells have been generated from many species including human, mice, porcine, and chicken albeit with only slight modifications. This chapter describes general considerations regarding critical aspects of their derivation compared with their counterpart, embryonic stem cells (ESCs). Detailed protocols for EGC derivation and maintenance from human and mouse primordial germ cells (PGCs) will be presented.
Kerr
Candace L
CL
Institute for Cell Engineering, Department of Obstetrics and Gynecology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Shamblott
Michael J
MJ
Gearhart
John D
JD
D053687:Adult Stem Cells; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D005854:Germ Cells; D006801:Humans; D039904:Pluripotent Stem Cells