Macrophages derived from bone marrow modulate differentiation of myeloid dendritic cells.
Dendritic cells (DC) are specialized antigen-presenting cells. Bone marrow monocytes have been widely used to generate murine myeloid DC. We found that mouse macrophages derived from bone marrow CD11b+ monocytes influenced the differentiation of these precursors into DC. Modulation of differentiation was demonstrated by the down-regulation of CD11c, CD40, and CD86 expression and by IL-12 production. DC differentiated in the presence of conditioned medium from bone marrow-derived macrophage culture (MCM) had impaired ability to stimulate proliferation of, and IFN- gamma production by, allogeneic CD4+ T cells. This inhibition of DC differentiation was mainly mediated by secretory products from macrophages but not by cell-cell contact. MCM contained higher concentrations of macrophage-colony-stimulating factor (M-CSF), IL-10, and TGF- beta1, whereas IL-6 remained unchanged compared with conditioned medium from fresh monocytes. M-CSF may be the major mediator in MCM inhibiting DC differentiation. This study demonstrates an important influence of bone marrow-derived macrophages on DC precursors during DC differentiation.
Liao
H-F
HF
Department of Molecular Biology and Biochemistry, National Chiayi University, Chiayi, Taiwan.
Yang
Y-C
YC
Chen
Y-Y
YY
Hsu
M-L
ML
Shieh
H-R
HR
Chen
Y-J
YJ
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D002454:Cell Differentiation; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology*; D018664:Interleukin-12 / Q000378:metabolism; D008213:Lymphocyte Activation; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D022423:Myeloid Cells / Q000166:cytology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
D016207:Cytokines; D018664:Interleukin-12