title: [The expression of HIF-1 in the early diabetic NOD mice].
abstract: OBJECTIVE
  To study the retinal expression of HIF-1 alpha in the diabetic NOD mice.
  
  
  METHODS
  NOD mice were divided into normal control group and diabetes group randomly, sacrificed at 2, 4, 6, 8 or 12 weeks. Eyes were enucleated and retinas were isolated and used for western blot, or eyes were sectioned for immunohistochemical staining for HIF-1 alphal.
  
  
  RESULTS
  Compared to the normal control group, blood sugar of diabetes mice increased significantly but the weights decreased significantly (P < 0.01). Western blot of retinal lysates showed low levels of HIF-1 alpha at the 6th weeks that were markedly increased at the 12th weeks in the diabetes group. Immunohistochemistry showed increased staining for HIF-1 in the inner retina, but not in the outer retina.
  
  
  CONCLUSIONS
  The retinal HIF-1 alpha levels increasing showed that retinal ischemia happening in the early diabetes. HIF-1 alpha may participate in the secondary damage of retinal ischemia induced by early diabetes.
authors.affiliation: Department of Ophthalmology, Xiangya Second Hospital of Central South University, Changsha 410007, China.
authors.forename: Cairui
authors.initials: C
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Deyong
authors.initials: D
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Hong
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Guo
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Wengling
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D003930:Diabetic Retinopathy / Q000378:metabolism; D005260:Female; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis*; D051379:Mice; D016688:Mice, Inbred NOD; D012160:Retina / Q000378:metabolism* / Q000473:pathology
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit