Fluorescent probe for the detection of DNA conformational transition.
Two pyrene-labeled bases, (py)C and (8py)G, have been synthesized and incorporated into d(CG)n. In the CD spectra, structural transition of a modified oligodeoxynucleotide (ODN-Py) from B-form to Z-form has been observed under different salt concentrations. The conformational transition of ODN-Py was monitored by the change of emission wavelengths based on exciplex and monomer fluorescence of (py)C and (8py)G.
Ochi
Yuji
Y
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan.
Okamoto
Akimitsu
A
Saito
Isao
I
D003596:Cytosine / Q000138:chemical synthesis / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006147:Guanine / Q000138:chemical synthesis / Q000737:chemistry; D009690:Nucleic Acid Conformation; D011721:Pyrenes / Q000138:chemical synthesis / Q000737:chemistry
D005456:Fluorescent Dyes; D011721:Pyrenes; D006147:Guanine; D003596:Cytosine; D004247:DNA; C030984:pyrene