Endogenous 15-deoxy-Delta(12,14)-prostaglandin J(2) synthesized by adipocytes during maturation phase contributes to upregulation of fat storage.
15-Deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) has been identified as a natural ligand for peroxisome proliferator-activated receptor (PPAR) gamma to promote adipogenesis. However, it remains elusive about the ability of PPARgamma-expressing adipocytes to produce PGJ(2) series and the role in the life cycle of adipocytes. Here, we developed an enzyme-linked immunosorbent assay specific for 15d-PGJ(2). The analysis using this method revealed the increase in the endogenous synthesis of immunoreactive 15d-PGJ(2) in cultured adipocytes during the maturation phase. Further studies using cyclooxygenase inhibitors clarified the contribution of endogeous 15d-PGJ(2) produced by mature adipocytes to upregulation of fat storage in an autocrine manner.
Mazid
Md Abdul
MA
Department of Life Science and Biotechnology, Shimane University, Matsue, Shimane 690-8504, Japan.
Chowdhury
Abu Asad
AA
Nagao
Kohjiro
K
Nishimura
Kohji
K
Jisaka
Mitsuo
M
Nagaya
Tsutomu
T
Yokota
Kazushige
K
D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D007527:Isoenzymes / Q000235:genetics; D051379:Mice; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000096:biosynthesis; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D015398:Signal Transduction; D015854:Up-Regulation
C097240:15-deoxy-delta(12,14)-prostaglandin J2; D015415:Biomarkers; D007527:Isoenzymes; D011451:Prostaglandin-Endoperoxide Synthases; D015230:Prostaglandin D2