title: An inward-facing conformation of a putative metal-chelate-type ABC transporter.
abstract: The crystal structure of a putative metal-chelate-type adenosine triphosphate (ATP)-binding cassette (ABC) transporter encoded by genes HI1470 and HI1471 of Haemophilus influenzae has been solved at 2.4 angstrom resolution. The permeation pathway exhibits an inward-facing conformation, in contrast to the outward-facing state previously observed for the homologous vitamin B12 importer BtuCD. Although the structures of both HI1470/1 and BtuCD have been solved in nucleotide-free states, the pairs of ABC subunits in these two structures differ by a translational shift in the plane of the membrane that coincides with a repositioning of the membrane-spanning subunits. The differences observed between these ABC transporters involve relatively modest rearrangements and may serve as structural models for inward- and outward-facing conformations relevant to the alternating access mechanism of substrate translocation.
authors.affiliation: Division of Chemistry and Chemical Engineering, Howard Hughes Medical Institute, MC 114-96, California Institute of Technology (Caltech), Pasadena, CA 91125, USA.
authors.forename: H W
authors.initials: HW
authors.lastname: Pinkett
authors.forename: A T
authors.initials: AT
authors.lastname: Lee
authors.forename: P
authors.initials: P
authors.lastname: Lum
authors.forename: K P
authors.initials: KP
authors.lastname: Locher
authors.forename: D C
authors.initials: DC
authors.lastname: Rees
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D019281:Dimerization; D006193:Haemophilus influenzae / Q000737:chemistry*; D008670:Metals / Q000378:metabolism; D008958:Models, Molecular; D011487:Protein Conformation; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D008670:Metals; D021122:Protein Subunits