title: Two-dimensional gel electrophoresis based technologies for potential biomarkers identification in amniotic fluid: a simple model.
abstract: To assess the efficiency of two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and of two-dimensional electrophoresis and ammoniacal silver staining (2D-E), different amounts of soybean trypsin inhibitor and horse myoglobin were added to amniotic fluid. In this model, a minimum of 5 to 10 ng of "artificial" biomarkers was detected.
authors.affiliation: Service régional vaudois de Transfusion Sanguine, Lausanne, Switzerland.
authors.forename: P-A
authors.initials: PA
authors.lastname: Queloz
authors.forename: D
authors.initials: D
authors.lastname: Crettaz
authors.forename: L
authors.initials: L
authors.lastname: Thadikkaran
authors.forename: V
authors.initials: V
authors.lastname: Sapin
authors.forename: J-D
authors.initials: JD
authors.lastname: Tissot
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis* / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008954:Models, Biological; D011247:Pregnancy; D016622:Silver Staining
chemical_list: D015415:Biomarkers