title: Kinetics and thermodynamics of G-quadruplexes.
abstract: The melting of tetramolecular DNA or RNA quadruplexes is kinetically irreversible. However, rather than being a hindrance, this kinetic inertia allows us to study association and dissociation processes independently. General rules have been extended to longer DNA motifs or sequences containing modified bases such as 8-oxo or 7-deaza guanine. Results were compared with the canonical TG4T and TG5T tetramers: we demonstrate huge differences (up to 10(5)-fold) in the association constants of these quadruplexes depending on primary sequence.
authors.affiliation: Laboratoire de Biophysique, INSERM U565, CNRS UMR5153, Muséum National d'Histoire Naturelle, 43 rue Cuvier, 75005 Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Gros
authors.forename: Mateus
authors.initials: M
authors.lastname: Webba da Silva
authors.forename: Anne
authors.initials: A
authors.lastname: De Cian
authors.forename: Samir
authors.initials: S
authors.lastname: Amrane
authors.forename: Frédéric
authors.initials: F
authors.lastname: Rosu
authors.forename: Anne
authors.initials: A
authors.lastname: Bourdoncle
authors.forename: Barbara
authors.initials: B
authors.lastname: Saccà
authors.forename: Patrizia
authors.initials: P
authors.lastname: Alberti
authors.forename: Laurent
authors.initials: L
authors.lastname: Lacroix
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Mergny
mesh_terms: D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D006147:Guanine / Q000031:analogs & derivatives / Q000737:chemistry*; D007700:Kinetics; D013816:Thermodynamics
chemical_list: C024829:8-hydroxyguanine; D006147:Guanine; D004247:DNA; C066856:7-deazaguanine