Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography-electrospray mass spectrometry.
The analysis of albendazole sulfoxide, albendazole sulfone, praziquantel and trans-4-hydroxypraziquantel in plasma was carried out by high-performance liquid chromatography-mass spectrometry ((LC-MS-MS). The plasma samples were prepared by liquid-liquid extraction using dichloromethane as extracting solvent. The partial HPLC resolution of drug and metabolites was obtained using a cyanopropyl column and a mobile phase consisting of methanol:water (3:7, v/v) plus 0.5% of acetic acid, at a flow rate of 1.0 mL/min. Multi reaction monitoring detection was performed by electrospray ionization in the positive ion mode, conferring additional selectivity to the method. Method validation showed relative standard deviation (precision) and relative errors (accuracy) lower than 15% for all analytes evaluated. The quantification limit was 5 ng/mL and the linear range was 5-2500 ng/mL for all analytes. The method was used for the determination of drug and metabolites in swine plasma samples and proved to be suitable for pharmacokinetic studies.
Bonato
Pierina Sueli
PS
Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. psbonato@fcfrp.usp.br
de Oliveira
Anderson Rodrigo Moraes
AR
de Santana
Fernando José Malagueño
FJ
Fernandes
Bruno José Dumet
BJ
Lanchote
Vera Lucia
VL
Gonzalez
Armando E
AE
Garcia
Hector H
HH
Takayanagui
Osvaldo Massaiti
OM
D015766:Albendazole / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D000871:Anthelmintics / Q000097:blood* / Q000493:pharmacokinetics; D000980:Antiplatyhelmintic Agents / Q000097:blood* / Q000493:pharmacokinetics; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D011223:Praziquantel / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013552:Swine
D000871:Anthelmintics; D000980:Antiplatyhelmintic Agents; D007202:Indicators and Reagents; C027187:albendazole sulfone; D011223:Praziquantel; D015766:Albendazole; C027186:albendazole sulfoxide