title: UVA-induced degradation of 8-hydroxyguanine in oligonucleotide and the effect on its activities in yeast oligonucleotide transformation assay.
abstract: UVA-induced conversion of 8-hydroxyguanine in oligonucleotides was studied. By irradiation with 334 nm UVA light, 8-hydroxyguanine was completely changed to unknown compounds. Monomeric nucleoside may be much less labile to UVA. Mutagenic specificities of 8-hydroxyguanine were investigated using yeast oligonucleotide mutation assay. UVA irradiation moderately reduced the activity of the oligonucleotides.
authors.affiliation: Department of Genomics and Proteomics, Okayama University Advanced Science Research Center, Tsushima, Okayama 700-8530, Japan.
authors.forename: Yoji
authors.initials: Y
authors.lastname: Okugawa
authors.forename: Chie
authors.initials: C
authors.lastname: Otsuka
authors.forename: David
authors.initials: D
authors.lastname: Loakes
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Negishi
mesh_terms: D001681:Biological Assay / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D016296:Mutagenesis; D009841:Oligonucleotides / Q000378:metabolism* / Q000528:radiation effects*; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D009841:Oligonucleotides; C024829:8-hydroxyguanine; D006147:Guanine