The influence of stress and methionine-enkephalin on macrophage functions in two inbred rat strains.
The aim of our current study was to investigate the effect of acute exposure to electric tail shock stress (ES) and to a stress witnessing procedure (SW), as models for physical and psychological stress paradigms, respectively, on phagocytosis and H(2)O(2) production in peritoneal macrophages isolated from Albino Oxford (AO) and Dark Agouti (DA) rats. In addition, we studied the in vitro effects of methionine-enkephalin (ME) on phagocytosis and H(2)O(2) production in peritoneal macrophages isolated from both AO and DA rats that had been exposed to ES and SW procedures. The results showed that peritoneal macrophages isolated from DA rats were less sensitive to the suppressive effects of ES and SW than macrophages isolated from AO rats. In vitro treatment of macrophages isolated from AO rats with ME mimicked to some extent the suppressive effects of ES and SW on phagocytosis and H(2)O(2) production and additionally diminished H(2)O(2) release in macrophages isolated from AO rats previously exposed to ES or SW. ME did not have any effect on phagocytosis in macrophages isolated from DA rats, but changed H(2)O(2) production in a concentration-dependent manner. In macrophages isolated from DA rats previously exposed to stress the effect of ME was dependent on the macrophage function tested and the particular stress paradigm employed. Our results emphasise the fact that both beneficial and detrimental effects of stress on immune system functions could be attributed to the individual variations in the macrophage's response to stress mediators.
Stanojević
Stanislava
S
Immunology Research Center Branislav Janković, Institute of Immunology and Virology Torlak, Vojvode Stepe 458, 11152 Belgrade, Serbia. canac@EUnet.yu
Mitić
Katarina
K
Vujić
Vesna
V
Kovacević-Jovanović
Vesna
V
Dimitrijević
Mirjana
M
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004597:Electroshock; D004744:Enkephalin, Methionine / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects; D010641:Phenotype; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology; D013045:Species Specificity; D013315:Stress, Psychological / Q000276:immunology*
D011956:Receptors, Cell Surface; D004744:Enkephalin, Methionine; D006861:Hydrogen Peroxide