Structural and functional comparison of native pentameric, denatured monomeric and biotinylated C-reactive protein.
There are many controversies surrounding the biological activities of native C-reactive protein (nCRP) and its various modified forms such as monomerized and biotinylated CRP (mCRP and bCRP). No simple methods have been described to distinguish among these forms. By adapting established electrophoresis methods, we have developed a useful quality control method with which we have investigated the structural and functional characteristics of these forms of CRP. Under all electrophoresis conditions, biotinylation altered the electrophoretic mobility of CRP. nCRP was sensitive to sodium dodecyl sulphate (SDS)-induced monomerization, and only mCRP was susceptible to digestion by trypsin or neutrophil-derived serine proteases. bCRP and mCRP but not nCRP bound to cells, suggesting that chemical modification by biotin and denaturation had altered the structural integrity of CRP. Neither nCRP nor mCRP had the ability to induce secretion of chemokines, nor did they increase intracellular adhesion molecule 1 (ICAM-1) expression in endothelial cells.
Taylor
Karolina E
KE
Department of Pharmacology, Therapeutics and Toxicology, Cardiff University, Wales College of Medicine, Cardiff, UK.
van den Berg
Carmen W
CW
D001710:Biotin; D002097:C-Reactive Protein / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D011489:Protein Denaturation; D012697:Serine Endopeptidases; D013329:Structure-Activity Relationship; D014357:Trypsin
D001710:Biotin; D002097:C-Reactive Protein; D012697:Serine Endopeptidases; D014357:Trypsin