[Effects of NO-synthase inhibitors on maturation mouse oocytes in cumulus-oocyte complexes].
We studied the effects of various NO-synthase inhibitors on meioitic maturation of mouse oocytes in vitro in cumulus-oocyte complexes isolated from follicles of varying sizes. Selective and nonselective inhibitors of NO-synthase isoforms suppressed meiotic maturation of oocytes to varying degrees, which was expressed in a decreased number of oocytes at metaphase II. The results obtained suggest that the role of inducible form of NO-synthase (iNOS) increases with the development of follicles and oocytes.
Voznesenskaia
T Iu
TIu
Blashkiv
T V
TV
D000818:Animals; D005260:Female; D006146:Guanidines / Q000494:pharmacology*; D066298:In Vitro Techniques; D008540:Meiosis; D008677:Metaphase; D051379:Mice; D008808:Mice, Inbred CBA; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019335:Nitroarginine / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D009866:Oogenesis / Q000187:drug effects*; D006080:Ovarian Follicle / Q000166:cytology
D006146:Guanidines; D019335:Nitroarginine; D019001:Nitric Oxide Synthase; C004479:pimagedine; D019331:NG-Nitroarginine Methyl Ester