Highly active artificial restriction enzyme composed of Ce(IV)/EDTA and PNA bearing phosphate group--relationship between the promotion by phosphate and the structure of invasion complex.
Recently, we developed artificial restriction DNA cutter (ARCUT) composed of pseudo-complementary peptide nucleic acid (pcPNA) and Ce(IV)/EDTA complex (EDTA = ethylenediamine-N,N,N',N'-tetraacetate). Here we promoted the site-selective hydrolysis by attaching phosphate groups to the pcPNAs. The promotion by the phosphates increased with decreasing length of the gap-like site. Furthermore, the scission was successful even when phosphate groups were introduced to 0 base-gap system.
Aiba
Yuichiro
Y
Research center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Yamamoto
Yoji
Y
Komiyama
Makoto
M
D002563:Cerium / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000737:chemistry* / Q000378:metabolism; D004492:Edetic Acid / Q000737:chemistry*; D006868:Hydrolysis; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry
D020135:Peptide Nucleic Acids; D010710:Phosphates; D002563:Cerium; D004247:DNA; D004492:Edetic Acid; D004262:DNA Restriction Enzymes