title: Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation.
abstract: Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.
authors.affiliation: Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonnington Campus, Loughborough LE12 5RD, UK.
authors.forename: Victoria
authors.initials: V
authors.lastname: Sherwood
authors.affiliation: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: Hans-Gerhard
authors.initials: HG
authors.lastname: Burgert
authors.affiliation: School of Biology, University of St Andrews, Fife KY16 9TS, UK.
authors.forename: Yun-Hsiang
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonnington Campus, Loughborough LE12 5RD, UK.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Sanghera
authors.affiliation: Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonnington Campus, Loughborough LE12 5RD, UK.
authors.forename: Socrates
authors.initials: S
authors.lastname: Katafigiotis
authors.affiliation: School of Biology, University of St Andrews, Fife KY16 9TS, UK.
authors.forename: Richard E
authors.initials: RE
authors.lastname: Randall
authors.affiliation: Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonnington Campus, Loughborough LE12 5RD, UK.
authors.forename: Ian
authors.initials: I
authors.lastname: Connerton
authors.affiliation: Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonnington Campus, Loughborough LE12 5RD, UK.
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Mellits
mesh_terms: D000260:Adenoviruses, Human / Q000254:growth & development* / Q000502:physiology; D002460:Cell Line; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D050794:STAT1 Transcription Factor / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D050794:STAT1 Transcription Factor; D007372:Interferons