MinK gene polymorphism in the pathogenesis of lone atrial fibrillation.
BACKGROUND
Atrial fibrillation (AF) is the most common type of complex arrhythmia found in everyday clinical practice. Lone AF is a particular form occurring in 2% to 31% of patients with confirmed AF. Genetic factors may underline this arrhythmia.


OBJECTIVE
To determine the relationship between G38S polymorphism in the MinK gene and the incidence of lone AF, and to evaluate this polymorphism as a genetic marker of susceptibility to AF.


METHODS
The study involved 69 patients with lone AF and 60 control healthy subjects. Both groups included patients aged up to 65 years without cardiovascular or thyroid disease. MinK genotype was determined with PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). The MinK gene was present in two allelic forms: G and S.


RESULTS
The MinK G allele was found significantly more often in patients with AF (62.32%) compared to control subjects (41.80%) (p=0.009). In the AF group GS occurred more frequently (55.07%) than GG (34.78%) and SS genotypes (10.14%). In a logistic regression model the presence of G variant was associated with increase of AF risk in the study population (OR 2.39; 95% CI 0.88-6.54; p=0.084). Presence of GG genotype was associated with significant, over 10-fold, increase of AF risk. Presence of S allele of the MinK gene met criteria of protective factor against AF in the study population.


CONCLUSIONS
1. G38S polymorphism in the MinK gene seems to be associated with incidence of lone AF in the study population. 2. GG genotype carrier state may significantly relate to increased risk of AF in the study group. 3. G38S polymorphism in the MinK gene could be used as a genetic marker of risk of lone AF.
Prystupa
Andrzej
A
Katedra i Klinika Chorób Wewnetrznych Akademii Medycznej im. prof. Feliksa Skubiszewskiego, Samodzielny Publiczny Szpital Kliniczny Nr 1, ul. Staszica 16, 20-081 Lublin. aprystupa@poczta.clinika.pl
Dzida
Grzegorz
G
Myśliński
Wojciech
W
Małaj
Grzegorz
G
Lorenc
Tomasz
T
D001281:Atrial Fibrillation / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics*
D005819:Genetic Markers; C489622:KCNE1 protein, human; D024642:Potassium Channels, Voltage-Gated; C059983:potassium channel protein I(sk)