title: NRAGE suppresses metastasis of melanoma and pancreatic cancer in vitro and in vivo.
abstract: We previously reported that human NRAGE could significantly alter the cellular skeleton and inhibit cell-cell adhesion, suggesting that human NRGAE play a potential role in cellular motility. Here, we report overexpression of human NRAGE in PANC-1 and B16-Bl6 cells could significantly suppress the metastasis of these cells in vitro and in vivo. Consistently, PANC-1 with stable silencing of NRAGE by RNA interference, exhibits a more metastatic phenotype than the native cell. Expression of epithelial proteins, including E-cadherin and beta-catenin is down regulated in siRNA-NRAGE PANC-1 cells. Further studies find that overexpression of human NRAGE suppresses the mRNA expression and activity of MMP2 significantly. Summary, our studies indicate for the first time that NRAGE could suppress metastasis of melanoma and pancreatic cancer probably through downregulation of MMP-2.
authors.affiliation: Jiangsu Key Laboratory for Molecular and Medical Biotechnology, Nanjing Normal University, Nanjing 210097, PR China.
authors.forename: Chao-shun
authors.initials: CS
authors.lastname: Chu
authors.forename: Bin
authors.initials: B
authors.lastname: Xue
authors.forename: Chen
authors.initials: C
authors.lastname: Tu
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Feng
authors.forename: Yi-huan
authors.initials: YH
authors.lastname: Shi
authors.forename: Yi
authors.initials: Y
authors.lastname: Miao
authors.forename: Chuan-Jun
authors.initials: CJ
authors.lastname: Wen
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement; D017931:DNA Primers; D020868:Gene Silencing; D006801:Humans; D066298:In Vitro Techniques; D008545:Melanoma / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D009363:Neoplasm Proteins / Q000235:genetics / Q000502:physiology*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000951:Antigens, Neoplasm; D017931:DNA Primers; C120897:MAGED1 protein, human; D009363:Neoplasm Proteins