Properties and EPR studies of oligodeoxynucleotides containing 2-N-tert-butylaminoxylpurines.
Oligodeoxynucleotide (ODN; 1) containing 2-N-tert-butylaminoxyladenosine (2) was synthesized by the conventional phosphoramidite method. The thermal stability and structure of the duplex of ODN 1 and the complementary strand were elucidated by UV-melting temperature measurements and CD experiments. In phosphate buffer, ODN 1 showed a three line EPR spectrum. Upon duplex formation, the EPR spectrum of 1 changed which indicates that EPR spectra of 1 can provide information on structures of ODN.
Aso
Mariko
M
Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Kaneko
Toshiyuki
T
Mirc
John Walter
JW
Koga
Noboru
N
Suemune
Hiroshi
H
D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011687:Purines / Q000737:chemistry; D013696:Temperature
C520181:2-N-tert-butylaminooxyladenosine; D009838:Oligodeoxyribonucleotides; D011687:Purines; D000241:Adenosine