title: Design of base-discriminating fluorescent (BDF) nucleobase for SNP typing.
abstract: We have devised novel pyrene-labeled BDF nucleosides, (Py)U, (Py)C, (Py)deazaA, and (Py)A. BDF probes containing these fluorescent nucleosides selectively emit fluorescence only when the base opposite the BDF base is a target base. Oligonucleotides containing these BDF nucleosides act as effective reporter probes for homogeneous SNP typing of DNA samples.
authors.affiliation: NEWCAT Institute, Faculty of Engineering, Nihon University and SORST, Japan Science and Technology Corporation, Tamura, Koriyama 963-8642, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Saito
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Kanatani
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ochi
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D024522:Genes, BRCA2; D006801:Humans; D015345:Oligonucleotide Probes / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes