[Variable expression of CD28 costimulatory molecule and CTLA4 inhibitory molecule on peripheral blood CD4+ cells in kidney allograft recipients with and without acute graft rejection].
Alloimune activation is one of the most significant post transplant events, which results in increased expression of costimulatory molecules. These molecules have been suggested to play a role in determining the outcome of immune response including graft rejection.


METHODS
We examined the CD28 and both surface and intracellular CTLA-4 expression on freshly drawn and anti-CD3+rlL-2 stimulated peripheral blood CD4+ T cells in kidney transplant recipients with acute graft rejection and with non-complicated post transplant course. Dual immunofluorescence and flow cytometry methods were used. The proportion of freshly isolated CD4+/ CTLA4 was higher in both groups of graft recipients in comparison to healthy controls reflecting in vivo allostimulation.


RESULTS
We found the increased percentage of CD4+ cells expressing surface CTLA4 after stimulation, unstimulated intracellular CTLA4 and lower percentage of CD4+ cells expressing CD28 after stimulation in kidney recipients without rejection.


CONCLUSIONS
Our results indicate the possible relationship between the expression pattern of CTLA4 inhibitory molecule on CD4+ cells and clinical course after renal transplantation.
Magott-Procelewska
Maria
M
Akademia Medyczna we Wrocławiu, Katedra i Klinika Nefrologii i Medycyny Transplantacyjnej. klinef@am.centrum.pl
Kosmaczewska
Agata
A
Frydecka
Irena
I
Ciszak
Lidia
L
Patrzałek
Dariusz
D
Szyber
Piotr
P
Klinger
Marian
M
D000208:Acute Disease; D015703:Antigens, CD / Q000096:biosynthesis* / Q000276:immunology; D000943:Antigens, Differentiation / Q000096:biosynthesis* / Q000276:immunology; D018106:CD28 Antigens / Q000096:biosynthesis* / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D060908:CTLA-4 Antigen; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology* / Q000473:pathology; D008297:Male; D012016:Reference Values; D012680:Sensitivity and Specificity; D014184:Transplantation, Homologous / Q000276:immunology* / Q000473:pathology
D015703:Antigens, CD; D000943:Antigens, Differentiation; D018106:CD28 Antigens; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human