title: Structural property and enzymatic response of oxanine in DNA strands.
abstract: Oxanine (Oxa, O), one of the major products generated from guanine (Gua) by nitrosative oxidation has been expected as mutagenic lesion involved in NO- or HNO(2) -induced genotoxicity. Here, to elucidate the biological meaning of Oxa in DNA strands, several kinds of Oxa-containing oligodeoxynucleotides (Oxa-ODNs) were synthesized and applied to biophysical and biochemical characterization. CD and NMR analyses revealed that the conformations of all the Oxa-containing duplexes are basically B-type without causing any severe distortion in the whole DNA structure. It was also determined that restriction endonucleases recognize and cleave the specific base-sequence even when Gua was substituted by Oxa in the sequence. When Oxa-ODN was testified as substrates for other DNA-relevant enzymes, the enzymatic functions were not largely affected by Oxa.
authors.affiliation: Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan.
authors.forename: Seung Pil
authors.initials: SP
authors.lastname: Pack
authors.forename: Akihiro
authors.initials: A
authors.lastname: Doi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nonogawa
authors.forename: Nagendra Kumar
authors.initials: NK
authors.lastname: Kamisetty
authors.forename: Kamakshaiah Charyulu
authors.initials: KC
authors.lastname: Devarayapalli
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kodaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Makino
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism; D011684:Purine Nucleosides / Q000737:chemistry*
chemical_list: D009838:Oligodeoxyribonucleotides; D011684:Purine Nucleosides; C468766:oxanine; D004247:DNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D004262:DNA Restriction Enzymes; D000469:Alkaline Phosphatase