Synthesis and properties of 2'-O-cyanoethylated RNA derivatives.
We developed a new protocol for the cyanoethylation of the hydroxyl function of nucleosides. A new system of Cs2CO3-tBuOH proved to be the best for the cyanoethylation. An oligonucleotide incorporating 2'-O-cyanoethylated uridine has high affinity (deltaTm = 0.6 degrees C/modification) for the complementary DNA or RNA strand than the corresponding 2'-O-methyl-RNA.
Saneyoshi
Hisao
H
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan.
Tamaki
Keigo
K
Seio
Kohji
K
Sekine
Mitsuo
M
D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry*; D013816:Thermodynamics
D012313:RNA