One-step separation and purification of 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography.
Three kinds of polyphenols of Salvia miltiorrhiza Bunge, 3,4-dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde, were separated and purified in one step with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (1:6:1.5:1.5:8) by high-speed counter-current chromatography. Acetic acid was successfully used to increase the partition of high polar target compounds in organic phase to modify partition coefficient value. 3,4-Dihydroxyphenyllactic acid, salvianolic acid B and protocatechualdehyde were purified from 100mg water extracted crude sample of Salvia miltiorrhiza Bunge at purity of 97.6%, 94.2% and 98.2% and at yield of 98.6%, 73.6% and 90.2%. High-speed counter-current chromatography together with organic/aqueous solvent system supplied an efficient method to purify water-soluble compounds directly from crude samples of traditional Chinese medicines.
Gu
Ming
M
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China. rainbow_gm@yahoo.com
Wang
Xiaolei
X
Su
Zhiguo
Z
Ouyang
Fan
F
D001547:Benzaldehydes / Q000302:isolation & purification*; D001572:Benzofurans / Q000302:isolation & purification*; D002396:Catechols / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D007773:Lactates / Q000302:isolation & purification*; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D053630:Depsides
D001547:Benzaldehydes; D001572:Benzofurans; D002396:Catechols; D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid; C005581:protocatechualdehyde; C076944:salvianolic acid B; D053630:Depsides