Promotion of cellular migration and apoptosis resistance by a mouse eye-specific phosphatidylethanolamine-binding protein.
The phosphatidylethanolamine (PE)-binding protein (PEBP) family is an evolutionary conserved group of proteins found in various species with multiple functions. We identified human PE-binding protein 4 (hPEBP4), a novel member of the PEBP family, as an anti-apoptotic molecule. Here we describe the cloning and functional characterization of the mouse homolog of hPEBP4 (mouse PEBP4, mPEBP4). Full-length cDNA of mPEBP4 contains 1048 bp with an open reading frame (ORF) of 729 bp, which is predicted to encode a 242-aa protein. mPEBP4 contains a PE-binding domain, in this case between amino acids 106 and 213. mPEBP4 localizes primarily to endoplasmic reticulum/Golgi apparatus. Interestingly, RT-PCR and in situ hybridization analyses indicate that mPEBP4 is specifically expressed in mouse eye tissue. We demonstrate that mPEBP4 promotes cellular migration and invasion by inhibiting ERK1/2 and JNK activation and up-regulating the expression of COX-2. In addition, mPEBP4 overexpression inhibits Epirubicin-induced cellular apoptosis. Considering that mPEBP4 is specifically expressed in retinal ganglion cells, whether mPEBP4 is an important molecule involved in visual function needs to be further investigated.
Zhang
Yuanyuan
Y
Institute of Immunology, Zhejiang University, Hangzhou 310031, PR China.
Wang
Xiaojian
X
Xiang
Zhenghua
Z
Li
Hongzhe
H
Qiu
Jianming
J
Sun
Qiaoling
Q
Wan
Tao
T
Li
Nan
N
Cao
Xuetao
X
Wang
Jianli
J
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002465:Cell Movement; D002478:Cells, Cultured; D003001:Cloning, Molecular; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015251:Epirubicin / Q000494:pharmacology; D005123:Eye / Q000378:metabolism*; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D051379:Mice; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D008969:Molecular Sequence Data; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
D002352:Carrier Proteins; C518018:PE-binding protein 4, human; D049488:Phosphatidylethanolamine Binding Protein; D012333:RNA, Messenger; D015251:Epirubicin; D051546:Cyclooxygenase 2; D048052:Mitogen-Activated Protein Kinase 3; D048670:MAP Kinase Kinase 4