Design of light-switchable phage promoter for efficient photo-regulation of gene-expression.
Efficient photoregulation of transcription by T7 RNA polymerase (RNAP) has been achieved with modified T7-promoter involving two azobenzene moieties at RNAP recognition region and TATA region simultaneously. Transcription proceeded 7.5-fold faster after UV irradiation than that under dark condition. Kinetic parameters (kcat, Km) for the transcription revealed that an azobenzene at each position played a different role and thus synergistic effect of photo-regulation was achieved with the promoter involving two azobenzenes.
Liu
Mingzhe
M
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Komiyama
Makoto
M
Asanuma
Hiroyuki
H
D001391:Azo Compounds / Q000737:chemistry; D017123:Bacteriophage T7 / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005786:Gene Expression Regulation / Q000528:radiation effects*; D007700:Kinetics; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000378:metabolism
D001391:Azo Compounds; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; C009850:azobenzene