[Effect of amino acids on cell proliferation and apoptosis in organotypic tissue culture of young and old rats].
There was studied in brain cortex, spleen and liver organotypic tissue culture the effect of effective concentrations (0.05 ng/ml) of 20 amino acids on the development of proliferative activity and apoptosis in peripheral fields of explants of rats of various age: 1 month and 18 months old. In the young tissues, the most frequent activity (3 times) was found while using the histidine, asparagine acide, valine, threonine, proline, leucine, tyrosine, methionine, and the less frequent (1 time)--while using lysine, glutamine, cysteine. Almost half number of the amino acids (11) was active in the old tissue cultures, as compared to the young tissues (19). The summary effectiveness of the amino acids was greater in young tissues (42), as compared to the old tissues (11). The data obtained suggest, that the bioregulator peptides should be necessary used to correct the less sensitivity of the tissues to the amino acids in old age.
Aniskina
A I
AI
Chalisova
N I
NI
Zakutskiĭ
A N
AN
Komashnia
A V
AV
Filippov
S V
SV
Zeziulin
P N
PN
D000375:Aging / Q000187:drug effects / Q000473:pathology*; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002540:Cerebral Cortex / Q000473:pathology*; D008099:Liver / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000473:pathology*; D046509:Tissue Culture Techniques
D000596:Amino Acids