title: Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil.
abstract: The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period.
authors.affiliation: Laboratório de Virologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, 74605-050 Goiânia, GO, Brazil.
authors.forename: Ana Maria Tavares
authors.initials: AM
authors.lastname: Borges
authors.forename: José Marcus Sócrates
authors.initials: JM
authors.lastname: Teixeira
authors.forename: Paulo Sérgio Sucasas da
authors.initials: PS
authors.lastname: Costa
authors.forename: Loreny Gimenes
authors.initials: LG
authors.lastname: Giugliano
authors.forename: Fabíola Souza
authors.initials: FS
authors.lastname: Fiaccadori
authors.forename: Rachel de Carvalho E
authors.initials: Rde C
authors.lastname: Franco
authors.forename: Wilia Marta Elsner Diederichsen de
authors.initials: WM
authors.lastname: Brito
authors.forename: José Paulo Gagliardi
authors.initials: JP
authors.lastname: Leite
authors.forename: Divina das Dores de Paula
authors.initials: Dd
authors.lastname: Cardoso
mesh_terms: D000208:Acute Disease; D017677:Age Distribution; D001938:Brazil / Q000453:epidemiology; D002139:Caliciviridae / Q000302:isolation & purification*; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D002675:Child, Preschool; D005243:Feces / Q000821:virology*; D005260:Female; D005759:Gastroenteritis / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D007223:Infant; D008297:Male; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons