[Methodological approaches to investigation of Na+-Ca2+ exchange in exocrine secretory cells].
An analysis of the methodological approaches, that used for investigation of Na+-Ca2+ exchange through the plasma membrane of exciting and secretory cells was presented in this review. Special attention is devoted to identification of Na+-Ca2+ exchange in the model for investigation of Ca2+ transporting systems of secretory cells - salivary glands of Chironomus plumosus L. larvae. With the aim different methods were used: researching of voltage-activated Ca2+-current depending on sodium gradient; studying of changes in the response of secretory glands, incubated in hypo- and hypersodium mediums, and Ca2+ content in their tissues; registration of Na+-Ca2+ exchange current in response to membrane hyper- or depolarisation changes of the membrane potential. And the current dependence on sodium and calcium ion gradient was also studied.
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D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002683:Chironomidae / Q000378:metabolism*; D005088:Exocrine Glands / Q000166:cytology / Q000378:metabolism*; D008564:Membrane Potentials; D012964:Sodium / Q000378:metabolism*
D012964:Sodium; D002118:Calcium