title: High throughput quantification of protein expression of cancer antigens in tissue microarray using quantum dot nanocrystals.
abstract: We developed and validated a novel method for quantifying protein expression of cancer tumors in an accurate, sensitive, and high throughput format. This technique integrates quantum dots, tissue microarray, optical spectroscopy, and algorithm design for analysis of tumor biopsies. The integration of this method for tissue analysis in the clinic bears potential impact for improving the diagnosis and treatment of cancer.
authors.affiliation: Division of Applied Molecular Oncology, Ontario Cancer Institute, Toronto, Ontario M5G 2M9, Canada.
authors.forename: Arezou A
authors.initials: AA
authors.lastname: Ghazani
authors.forename: Jeongjin A
authors.initials: JA
authors.lastname: Lee
authors.forename: Jesse
authors.initials: J
authors.lastname: Klostranec
authors.forename: Qing
authors.initials: Q
authors.lastname: Xiang
authors.forename: Ralph S
authors.initials: RS
authors.lastname: Dacosta
authors.forename: Brian C
authors.initials: BC
authors.lastname: Wilson
authors.forename: Ming S
authors.initials: MS
authors.lastname: Tsao
authors.forename: Warren C W
authors.initials: WC
authors.lastname: Chan
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D007633:Keratins / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D046228:Microarray Analysis; D009368:Neoplasm Transplantation; D045663:Quantum Dots; D014183:Transplantation, Heterologous
chemical_list: D000951:Antigens, Neoplasm; D015820:Cadherins; D007633:Keratins; D066246:ErbB Receptors