title: Desulfoglaeba alkanexedens gen. nov., sp. nov., an n-alkane-degrading, sulfate-reducing bacterium.
abstract: Two novel sulfate-reducing bacteria, strains ALDC(T) and Lake, which were able to oxidize n-alkanes, were isolated from a naval oily wastewater-storage facility (VA, USA) and from oilfield production water (OK, USA), respectively. The type strain (ALDC(T)) had a narrow substrate specificity and could grow only with n-alkanes (from C(6) to C(12)), pyruvate, butyrate, hexanoic acid and 4-methyloctanoic acid. Cells of strain ALDC(T) stained Gram-negative and were slightly curved, short rods with oval ends (2.5-3.0x1.0-1.4 microm), often occurring in pairs. Cells tended to form aggregates or large clusters and were non-motile and did not form endospores. Optimum growth occurred between 31 and 37 degrees C and at pH 6.5-7.2. NaCl was not required for growth, but salt concentrations up to 55 g l(-1) could be tolerated. The DNA G+C content was 53.6 mol%. Phylogenetic analysis of the 16S rRNA genes revealed that strains ALDC(T) and Lake were closely related, but not identical (99.9 % similarity). The two strains were not closely related to other known alkane-degrading, sulfate-reducing bacteria or to other genera of the Deltaproteobacteria. Therefore, it is proposed that strain ALDC(T) (=JCM 13588(T)=ATCC BAA-1302(T)) represents the type strain of a novel species and genus, with the name Desulfoglaeba alkanexedens gen. nov., sp. nov.
authors.affiliation: Department of Botany and Microbiology, Institute for Energy and the Environment, The University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
authors.forename: Irene A
authors.initials: IA
authors.lastname: Davidova
authors.affiliation: Department of Botany and Microbiology, Institute for Energy and the Environment, The University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
authors.forename: Kathleen E
authors.initials: KE
authors.lastname: Duncan
authors.affiliation: Department of Botany and Microbiology, Institute for Energy and the Environment, The University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
authors.forename: Ok Kyoung
authors.initials: OK
authors.lastname: Choi
authors.affiliation: Department of Botany and Microbiology, Institute for Energy and the Environment, The University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Suflita
mesh_terms: D000473:Alkanes / Q000378:metabolism*; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020564:Deltaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D009825:Oklahoma; D010084:Oxidation-Reduction; D010578:Petroleum; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D013379:Substrate Specificity; D013431:Sulfates / Q000378:metabolism*; D016999:Sulfur-Reducing Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods
chemical_list: D000473:Alkanes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013431:Sulfates