[Determination of acetylbritannilactone in inulicin and rat plasma by micellar electrokinetic capillary chromatography with on-line sweeping concentration technique].
A method was established for the determination of acetylbritannilactone (ABL) in inulicins and rat plasma by micellar electrokinetic capillary chromatography (MECC), in which on-line sweeping technique was applied to elevate the sensitivity of the detection. The column used was an uncoated fused silica capillary with a size of 57 cm (effective length of 50 cm) x 75 microm i.d. Electrode buffer was a 50 mmol/L borate buffer containing 50 mmol/L sodium lauryl sulfate (SDS) (pH 9.5). Sample buffer was a 10 mmol/L borate buffer (pH 9.5). Other conditions were as follows: separation voltage, 23 kV; detection wavelength, 195 nm; sample injection, 3.45 kPa (0.5 psi) x 5 s; column temperature, 25 degrees C. The on-line sweeping-MECC had excellent linearity with correlation coefficient of 0. 997 5, and reproducibility with the relative standard deviation of ABL migration time less than 5%. The recovery was more than 92%, and the sensitivity was 0.005 g/L. ABL in inulicin and their dynamic change in vivo were determined by sweeping-MECC. The results indicate that on-line sweeping-MECC is a simple and rapid method for the determination of trace biological sample. The method needs only micro-amount of samples, and is characterized by higher degree of automation and sensitivity.
Zhao
Jingshan
J
Key Laboratory for Medical Biotechnology of Hebei Province, Institute of Basic Medicine Science, Hebei Medical University, Shijiazhuang 050017, China. zjs10@sina.com
Wen
Jinkun
J
Han
Mei
M
D000818:Animals; D020374:Chromatography, Micellar Electrokinetic Capillary; D007783:Lactones / Q000032:analysis* / Q000097:blood; D051381:Rats; D012717:Sesquiterpenes / Q000737:chemistry*
D007783:Lactones; D012717:Sesquiterpenes; C487680:acetylbritannilatone; C031971:inulicin