title: Chronic Helicobacter pylori infection does not significantly alter the microbiota of the murine stomach.
abstract: We examined the impact of Helicobacter pylori infection on the murine gastric microbiota by culture and terminal-restriction fragment length polymorphism and found that neither acute nor chronic H. pylori infection substantially affected the gastric microbial composition. Interestingly, the total H. pylori burden detected by real-time PCR was significantly higher than that revealed by viable counts, suggesting that the antigenic load sustaining H. pylori-induced gastritis could be considerably higher than previously believed.
authors.affiliation: Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria 3010, Australia.
authors.forename: Mai Ping
authors.initials: MP
authors.lastname: Tan
authors.forename: Maria
authors.initials: M
authors.lastname: Kaparakis
authors.forename: Maja
authors.initials: M
authors.lastname: Galic
authors.forename: John
authors.initials: J
authors.lastname: Pedersen
authors.forename: Martin
authors.initials: M
authors.lastname: Pearse
authors.forename: Odilia L C
authors.initials: OL
authors.lastname: Wijburg
authors.forename: Peter H
authors.initials: PH
authors.lastname: Janssen
authors.forename: Richard A
authors.initials: RA
authors.lastname: Strugnell
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D002908:Chronic Disease; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013270:Stomach / Q000382:microbiology*