title: Rapid and highly sensitive determination of low-molecular-weight carbonyl compounds in drinking water and natural water by preconcentration HPLC with 2,4-dinitrophenylhydrazine.
abstract: The aim of this research was to develop a simple procedure for a highly sensitive determination of low-molecular-weight (LMW) carbonyl compounds in drinking water and natural water. We employed a preconcentration HPLC system with 2,4-dinitrophenylhydrazine (DNPH) for the determination of LMW carbonyl compounds. A C-18 reverse-phase preconcentration column was used instead of a sample loop at the sample injection valve. A 0.1 - 5.0 mL portion of the derivatized sample solution was injected with a gas-tight syringe, and a 15% acetonitrile aqueous solution was pushed through the preconcentration column to remove the unreacted excess DNPH, which caused serious interference in the determination of formaldehyde. The detection limits were 1 - 3 nM with a relative standard deviation of 2 - 5% for 20 nM standard solutions (n = 5). The calibration curves were essentially unaffected by coexisting sea salts. Applications to commercial mineral water, tap water, river water, pond water and seawater are presented.
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Japan. takedaq@hiroshima-u.ac.jp
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Takeda
authors.forename: Shinya
authors.initials: S
authors.lastname: Katoh
authors.forename: Nobutake
authors.initials: N
authors.lastname: Nakatani
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakugawa
mesh_terms: D000447:Aldehydes / Q000032:analysis; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005557:Formaldehyde / Q000032:analysis*; D010659:Phenylhydrazines; D014867:Water / Q000032:analysis*; D014873:Water Pollutants / Q000032:analysis
chemical_list: D000447:Aldehydes; D010659:Phenylhydrazines; D014873:Water Pollutants; D014867:Water; D005557:Formaldehyde; C004787:2,4-dinitrophenylhydrazine