title: [Assessment of bovine biomaterials containing bone morphogenetic proteins bound to absorbable hydroxyapatite in rabbit segmental bone defects].
abstract: OBJECTIVE
  To evaluate the osteo-regenerative capacity of two proprietary bone grafting materials, using a segmental defect model in both radial diaphyses of rabbits.
  
  
  METHODS
  The right defect was filled with pooled bone morphogenetic proteins (pBMPs) bound to absorbable ultrathin powdered hydroxyapatite (HA) mixed with inorganic and demineralized bone matrix and bone-derived collagen, derived from bovine bone (Group A). The left defect was filled with bovine demineralized bone matrix and pBMPs bound to absorbable ultrathin powdered HA (Group B). In both groups, an absorbable membrane of demineralized bovine cortical was used to retain the biomaterials in the bone defects, and to guide the tissue regeneration. The rabbits were euthanized 30, 90 and 150 days after surgery. Radiographic, tomographic and histologic evaluations were carried out on all specimens.
  
  
  RESULTS
  At 30 days, the demineralized cortical bone cover was totally resorbed in both groups. HA was totally resorbed from Group A defects, whereas HA persisted in Group B defects. A prominent foreign body reaction was evident with both products, more pronounced in sections from Group B. At 90 days, the defects in Group B exhibited more new bone than Group A. However, at 150 days after surgery, neither treatment had stimulated complete repair of the defect.
  
  
  CONCLUSIONS
  The partial bone healing of the segmental defect occurred with low or none performance of the biomaterials tested.
authors.affiliation: Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, UNESP, Botucatu, Brazil. caporali@uiuc.edu
authors.forename: Evelyn Hasegawa Gonçalves
authors.initials: EH
authors.lastname: Caporali
authors.forename: Sheila Canevese
authors.initials: SC
authors.lastname: Rahal
authors.forename: José
authors.initials: J
authors.lastname: Morceli
authors.forename: Rumio
authors.initials: R
authors.lastname: Taga
authors.forename: José Mauro
authors.initials: JM
authors.lastname: Granjeiro
authors.forename: Tânia M
authors.initials: TM
authors.lastname: Cestari
authors.forename: Maria Jaqueline
authors.initials: MJ
authors.lastname: Mamprim
authors.forename: Mariana A
authors.initials: MA
authors.lastname: Correa
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000637:transplantation; D019485:Bone Morphogenetic Proteins / Q000627:therapeutic use* / Q000648:ultrastructure; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D001842:Bone and Bones / Q000187:drug effects* / Q000601:surgery / Q000648:ultrastructure; D002417:Cattle; D003094:Collagen / Q000008:administration & dosage; D004195:Disease Models, Animal; D017886:Durapatite / Q000627:therapeutic use; D048091:Guided Tissue Regeneration / Q000379:methods; D011817:Rabbits; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D003094:Collagen; D017886:Durapatite