[The proteomics study of apoptotic NB4 cells induced by sodium butyrate].
OBJECTIVE
To explore the molecular mechanisms of apoptotic NB4 cells induced by the histone deacetylase inhibitor, sodium butyrate(SB).


METHODS
SB was exposed to NB4 cells at a final concentration of 1.0 mmol/L. The untreated and treated cells were analysed with FACS and 2-dimensional electrophoresis (2-DE) at 0, 12, 24, 48 and 72 h. The changed protein spots were identified with MALDI-TOF-MS and ESI-TOF-MS/MS.


RESULTS
SB induced apoptosis of NB4 cells. Twenty-one changed proteins involving apoptotic signal transduction, immunological regulation, transcriptional control, cellular metabolism, molecular transport and so on were identified. Thirteen of them had been reported to be related to apoptosis.


CONCLUSIONS
SB can induce apoptosis and many functional protein changes in tumor cells. These results pave the way to further explore the anti-tumor mechanism of SB.
Li
Wei
W
Department of Hematology/Oncology, First Hospital of Jilin University, Changchun 130021, China.
Wang
Guan-Jun
GJ
Cui
Jiu-Wei
JW
Chen
Xiao-Feng
XF
Zhang
Xue-Min
XM
D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D002087:Butyrates / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D040901:Proteomics
D002087:Butyrates