DNA recognition and recognition control of alpha-peptide ribonucleic acids by external factors.
Peptide ribonucleic acid (PRNA) is one of artificial nucleic acids, which enables us to control its nucleobase orientation and recognition behavior by external factors through the synergetic effects of reversible borate ester formation and intramolecular hydrogen bond formation. In this study, a series of novel alpha-PRNA oligomers, possessing alternative alpha-PRNA/basic amino acid sequences, were newly designed, synthesized, and evaluated as the second-generation PRNA. As expected, these alpha-PRNAs indeed formed highly stable sequence-specific complexes with the complementary DNAs, for which both the conventional hydrogen-bonding interactions between the complementary nucleobase pairs and the electrostatic interactions between the basic amino acid's ammonium cation and the DNA's phosphate anion on the backbone are jointly responsible.
Wada
Takehiko
T
Department of Applid Chemistry, Graduate School of Engineering, Osaka University, Yamada-oka, Suita 565-0871, Japan.
Kikkawa
Mayuko
M
Sato
Hirofumi
H
Inoue
Yoshihisa
Y
D002021:Buffers; D002942:Circular Dichroism; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011741:Pyrimidine Nucleosides / Q000737:chemistry*; D012263:Ribonucleosides / Q000138:chemical synthesis / Q000737:chemistry*
D002021:Buffers; D009838:Oligodeoxyribonucleotides; D020135:Peptide Nucleic Acids; D010455:Peptides; D011741:Pyrimidine Nucleosides; D012263:Ribonucleosides