[Subtype and sequence analysis of human immunodeficiency virus type-1 strains in Guangdong province].
OBJECTIVE
To investigate subtype and genetic analysis of human immunodeficiency virus type-1 (HIV-1).


METHODS
DNA sequences were amplified by nested-PCR from uncultured peripheral blood mononuclear cells (PBMC) obtained from 100 HIV-1 patients from Guangdong Province. The C2 to V3 region of the envelope glycoprotein gp120 of HIV-1 was sequenced directly. The analysis of the gene-based phylogenetic tree and variation of amino acid were carried out by using Wisconsin software package or genetics computer group (GCG).


RESULTS
DNA fragments were amplified from 75 PBMC samples by using nested polymerase chain reaction (PCR). Sequence analysis showed that there were 3 HIV-1 subtypes or circulating recombinant forms (CRF): CRF01-AE (n = 44), CRF-BC (n = 27) and B' (n = 4).


CONCLUSIONS
Three HIV-1 subtypes or circulating recombinant forms: CRF01-AE, CRF-BC and B' might be circulating in Guangdong Province. Findings from this study suggested that several subtypes might exist in Guangdong Province and the epidemic situation of AIDS be serious. It should be a challenge for Guangdong Province in treating patients, preventing and controlling AIDS in the future.
Wan
Zhuo-yue
ZY
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 510300, China.
Xing
Hui
H
Li
Jie
J
Wang
Yu
Y
Diao
Li-mei
LM
Ma
Peng-fei
PF
Lin
Peng
P
Jiang
Shu-lin
SL
Shao
Yi-ming
YM
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D002648:Child; D002681:China / Q000453:epidemiology; D004279:DNA, Viral; D005260:Female; D015497:HIV-1 / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020033:Protein Isoforms
D004279:DNA, Viral; D020033:Protein Isoforms