title: Comparison of artificial synthesis methods of gene.
abstract: The comparative study of the methods for constructing the artificial genes have done. Equine interferon alpha-1 gene and porcine interferon alpha-1 gene were synthesized respectively by several methods from short DNA oligomers and each gene was evaluated about the accuracy of the gene sequence. The gene made by the SPR method was the most accurate.
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, Kodaira, Tokyo, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Tsuchiya
authors.forename: Kazuki
authors.initials: K
authors.lastname: Morioka
authors.forename: Junsuke
authors.initials: J
authors.lastname: Shirai
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yoshida
authors.forename: Shigeki
authors.initials: S
authors.lastname: Inumaru
mesh_terms: D000818:Animals; D004259:DNA-Directed DNA Polymerase; D005813:Genes, Synthetic; D005821:Genetic Techniques; D006736:Horses / Q000235:genetics; D016898:Interferon-alpha / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D013552:Swine / Q000235:genetics
chemical_list: D016898:Interferon-alpha; D009838:Oligodeoxyribonucleotides; D004259:DNA-Directed DNA Polymerase