Development of a new diagnostic method for galactosemia by high-performance anion-exchange chromatography with pulsed amperometric detection.
We developed a new non-derivatization analytical method for the determination of galactose in the diagnosis of galactosemia by high-performance anion-exchange chromatography (HPAEC)-pulsed amperometric detection (PAD). With an anion-exchange column, the analytes were separated efficiently using 3mM NaOH containing 1mM NaOAc, and 200mM NaOH was added for post-column reagent. The limit of detection (S/N=3) and limit of quantification (S/N=10) for galactose were 25ng/mL and 83ng/mL, respectively. Linear dynamic range was from 4.67mg/dL to 53.46mg/dL (r(2)=0.9999). The mean recovery of galactose for intra-, inter-day assays were found to be of satisfactory results (98.14-101.42%).
Jeong
Ji-Seon
JS
Department of Oriental Pharmaceutical Sciences, Kyung Hee University, Dongdaemoon-gu Hoegi-dong #1, Seoul 130-701, South Korea.
Yoon
Hye-Ran
HR
Hong
Seon-Pyo
SP
D001786:Blood Glucose / Q000032:analysis; D001800:Blood Specimen Collection; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004563:Electrochemistry; D005690:Galactose / Q000097:blood*; D005693:Galactosemias / Q000175:diagnosis*; D006801:Humans; D007231:Infant, Newborn; D012016:Reference Values; D015203:Reproducibility of Results; D006599:UDPglucose-Hexose-1-Phosphate Uridylyltransferase / Q000172:deficiency
D001786:Blood Glucose; D006599:UDPglucose-Hexose-1-Phosphate Uridylyltransferase; D005690:Galactose