title: Human PEP-1-ribosomal protein S3 protects against UV-induced skin cell death.
abstract: The consequences of ultraviolet (UV) exposure are implicated in skin aging and cell death. The ribosomal protein S3 (rpS3) is one of the major proteins by which cells counteract the deleterious effects of UV and it plays a role in the repair of damaged DNA. In the present study, we investigated the protective effects of PEP-1-rpS3 fusion protein after UV-induced cell injury. A human rpS3 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-rpS3 fusion protein. The expressed and purified fusion proteins were efficiently transduced into skin cells in a time- and dose-dependent manner. Once inside the cells, transduced PEP-1-rpS3 fusion protein was stable for 48h. We showed that transduced PEP-1-rpS3 fusion protein increased cell viability and dramatically reduced DNA lesions in the UV exposed skin cells. Immunohistochemical analysis revealed that PEP-1-rpS3 fusion protein efficiently penetrated the epidermis as well as the dermis of the subcutaneous layer when sprayed on animal skin. These results suggest that PEP-1-rpS3 fusion protein can be used in protein therapy for various disorders related to UV, including skin aging and cancer.
authors.affiliation: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Republic of Korea.
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Choi
authors.forename: So Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Jae Jin
authors.initials: JJ
authors.lastname: An
authors.forename: Sun Hwa
authors.initials: SH
authors.lastname: Lee
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Hea Jin
authors.initials: HJ
authors.lastname: Ryu
authors.forename: Nam Il
authors.initials: NI
authors.lastname: Lee
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Yeo
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Jang
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Joon
authors.initials: J
authors.lastname: Kim
authors.forename: Kil Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D003543:Cysteamine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D004249:DNA Damage / Q000528:radiation effects; D005347:Fibroblasts; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D051379:Mice; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D012269:Ribosomal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D012867:Skin / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: C503924:Pep-1 peptide; D010455:Peptides; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; C044152:ribosomal protein S3; D003543:Cysteamine