Delay of leaf senescence in Medicago sativa transformed with the ipt gene controlled by the senescence-specific promoter SAG12.
We report the successfull delay of leaf senescence in Medicago sativa. A highly regenerable clone of alfalfa was transformed with the construct SAG12-IPT, an approach that has already proved efficient in other crops. Several independent transformants were obtained as determined by Southern analysis and all the transformants expressed the transgene as measured by RT-PCR. In vitro and in vivo analyses showed that SAG12-IPT plants exhibited a stay-green phenotype that has the potential to greatly improve the quantity and quality of alfalfa forage.
Calderini
Ornella
O
CNR Istituto di Genetica Vegetale, via Madonna Alta 130, Perugia, Italy.
Bovone
Tessa
T
Scotti
Carla
C
Pupilli
Fulvio
F
Piano
Efisio
E
Arcioni
Sergio
S
D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D016922:Cellular Senescence; D002734:Chlorophyll / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D000455:Medicago sativa / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D018515:Plant Leaves / Q000201:enzymology / Q000502:physiology*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014170:Transformation, Genetic
D002734:Chlorophyll; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase