Genistein attenuates postischemic ovarian injury in a rat adnexal torsion-detorsion model.
OBJECTIVE
To investigate the effects of genistein on reperfusion injury in a rat ovarian torsion-detorsion model.


METHODS
Controlled experimental study.


METHODS
University animal research laboratory.


METHODS
Thirty-two Wistar-Albino rats.


METHODS
The rats were divided into four groups. Sham operation was performed in group I. In group II, 5 mg/kg genistein was given intraperitoneally (IP) during laparotomy, and right ovaries were removed 4 hours later. In group III, right ovaries were subjected to 4 hours of adnexal ischemia by use of vascular clips, and after ischemic insult, 4 hours of reperfusion was maintained by removing the clips. In group IV, after the ischemic period, 5 mg/kg genistein was given IP, and 4 hours of reperfusion was maintained. Right ovaries were surgically removed at the end of the procedure in each group.


METHODS
Ovarian histopathologic findings were scored and compared among study groups. Serum and ovarian tissue malondialdehyde (MDA), glutathione peroxidase, and superoxide dismutase, levels were measured.


RESULTS
Ovarian tissue damage scores were significantly different among groups and were seen to correlate with ovarian tissue MDA levels. Genistein significantly decreased the tissue damage scores, ovarian tissue MDA levels, and serum MDA levels.


CONCLUSIONS
Genistein attenuates ischemia-reperfusion injury in rat adnexal torsion-detorsion model.
Yazici
Gurkan
G
Department of Obstetrics and Gynecology, School of Medicine, Mersin University, Mersin, Turkey. gyazici70@yahoo.com <gyazici70@yahoo.com>
Erdem
Ozlem
O
Cimen
Burak
B
Arslan
Murat
M
Tasdelen
Bahar
B
Cinel
Ismail
I
D000291:Adnexal Diseases / Q000150:complications / Q000188:drug therapy / Q000473:pathology; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D019833:Genistein / Q000008:administration & dosage*; D010049:Ovarian Diseases / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology*; D010053:Ovary / Q000098:blood supply / Q000187:drug effects* / Q000293:injuries / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy / Q000473:pathology; D014102:Torsion Abnormality / Q000150:complications / Q000188:drug therapy / Q000473:pathology; D016896:Treatment Outcome
D019833:Genistein