title: blaVIM-2 and blaVIM-7 carbapenemase-producing Pseudomonas aeruginosa isolates detected in a tertiary care medical center in the United States: report from the MYSTIC program.
abstract: Two Pseudomonas aeruginosa strains resistant to beta-lactams, fluoroquinolones, aminoglycosides, tetracyclines, and carbapenems and susceptible only to polymyxin B (MIC <or= 2 microg/ml) were identified as part of the Meropenem Yearly Susceptibility Test Information Collection program. Metallo-beta-lactamase screening tests were positive, PCR yielded products with blaVIM primers, and sequence analysis revealed blaVIM-7 and blaVIM-2. The isolates had distinct ribotype and pulsed-field gel electorphoresis patterns and appeared independently, remote in time and location, at the same cancer center.
authors.affiliation: The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: H
authors.initials: H
authors.lastname: Aboufaycal
authors.forename: H S
authors.initials: HS
authors.lastname: Sader
authors.forename: K
authors.initials: K
authors.lastname: Rolston
authors.forename: L M
authors.initials: LM
authors.lastname: Deshpande
authors.forename: M
authors.initials: M
authors.lastname: Toleman
authors.forename: G
authors.initials: G
authors.lastname: Bodey
authors.forename: I
authors.initials: I
authors.lastname: Raad
authors.forename: R N
authors.initials: RN
authors.lastname: Jones
mesh_terms: D000046:Academic Medical Centers; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D013781:Texas; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C425108:beta-lactamase bla(vim-2); C481670:VIM-7 metallo-beta-lactamase; D001618:beta-Lactamases; C063912:carbapenemase