Screening of modified DNA aptamers that recognize DNA secondary structure.
We attempted to utilize in vitro selection techniques to develop modified DNA aptamers that are specific for DNA secondary structure including a single-mismatch base pair. For the selection, two kinds of random modified DNA library were prepared by an enzymatic method using modified 2'-deoxynucleoside 5'-triphosphates and KOD Dash DNA polymerase: one library consists of modified DNA including amino functionality and another consists of modified DNA including both hydroxypropynyl and guanidium functionalities.
Kuwahara
Masayasu
M
Department of Applied Chemistry, Gunma University, Kiryu, Gunma 376-8515, Japan.
Kasamatsu
Toshiyuki
T
Hasegawa
Masatoshi
M
Shoji
Atsushi
A
Ozaki
Hiroaki
H
Sawai
Hiroaki
H
D052157:Aptamers, Nucleotide / Q000302:isolation & purification*; D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D015723:Gene Library; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D052156:SELEX Aptamer Technique / Q000379:methods*
D052157:Aptamers, Nucleotide; D004247:DNA; D004259:DNA-Directed DNA Polymerase