Immobilization of lipase on epoxy activated (1-->3)-alpha-D-glucan isolated from Penicillium chrysongenum.
A water-insoluble linear (1-->3)-alpha-D-glucan was isolated from Penicillium mycelia. Three kinds of epoxy-activated microspheres of this glucan were prepared as supports for Candida sp. lipase (EC3.1.1.3) immobilization. The highest immobilization yield was 36.4%. The specific activity was 26.85 U/mg, and only 4.1% of activity was lost in comparison with the free enzyme used for immobilization. The higher thermal stability, storage stability, and reusability of the immobilized lipase make it a potential candidate for wide application.
Wang
Tianqi
T
Beijing Key Lab of Biochemical Engineering, Beijing University of Chemical Technology.
Li
Hanxiang
H
Nie
Kaili
K
Tan
Tianwei
T
D004800:Enzymes, Immobilized / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D010408:Penicillium chrysogenum / Q000378:metabolism*; D013055:Spectrophotometry, Infrared
D004800:Enzymes, Immobilized; D005936:Glucans; C045788:alpha-1,3-glucan; D008049:Lipase