Characterization of a novel oncogenic K-ras mutation in colon cancer.
Activating mutations of RAS are frequently observed in subsets of human cancers, indicating that RAS activation is involved in tumorigenesis. Here, we identified and characterized a novel G to T transversion mutation of the K-ras gene at the third position of codon 19 (TTG) which substituted phenylalanine for leucine in 3 primary colon carcinomas. Biological and biochemical activity was examined using transformed NIH3T3 cells expressing mutant or wild-type K-ras. Transformants harboring the K-ras mutation at codon 19 showed proliferative capacity under serum-starved conditions, less contact inhibition, anchorage-independent growth, tumorigenicity in nude mice and elevation of active Ras-GTP levels. These results indicated that this novel mutation possesses high oncogenic activity.
Akagi
Kiwamu
K
Molecular Diagnosis and Cancer Prevention Division, Saitama Cancer Center, 818 Komuro Ina, Kitaadachigun, Saitama 362-0806, Japan. akagi@cancer-c.pref.saitama.jp
Uchibori
Ryosuke
R
Yamaguchi
Kensei
K
Kurosawa
Keiko
K
Tanaka
Yoichiro
Y
Kozu
Tomoko
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism*; D011905:Genes, ras / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics*; D018631:ras Proteins / Q000235:genetics*
D009363:Neoplasm Proteins; D018631:ras Proteins