title: High-resolution melting assay for the detection of gyrA mutations causing quinolone resistance in Salmonella enterica serovars Typhi and Paratyphi.
abstract: We developed a novel rapid assay to detect the gyrA mutations that cause quinolone resistance in typhoid and paratyphoid fever Salmonella spp. using high-resolution melting (Idaho Technology, Salt Lake City, UT) analysis of polymerase chain reaction amplicons. The presence of gyrA mutations led to small but consistent changes in amplicon melting temperatures that allowed quinolone-resistant isolates to be differentiated from susceptible ones.
authors.affiliation: Division of Bacteriology, Department of Pathology and Laboratory Medicine, Children's Hospital of Eastern Ontario, Ottawa, ON, Canada K1H 8L1. slinger@cheo.on.ca
authors.forename: Robert
authors.initials: R
authors.lastname: Slinger
authors.forename: Deana
authors.initials: D
authors.lastname: Bellfoy
authors.forename: Marc
authors.initials: M
authors.lastname: Desjardins
authors.forename: Francis
authors.initials: F
authors.lastname: Chan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D027081:DNA Gyrase / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D015363:Quinolones / Q000494:pharmacology; D012482:Salmonella paratyphi A / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D012485:Salmonella typhi / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D044366:Transition Temperature
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015363:Quinolones; D027081:DNA Gyrase