Synthesis of ferrocenyl carbodiimide as a novel ferrocenyl reagent of single stranded DNA.
Ferrocenyl carbodiimide (FcCDI) was newly synthesized as a ferrocenylation reagent for single stranded DNA. FcCDI could attach to oligodeoxyribonucleotides (ODN) through a covalent bond with thymine and guanine bases. The reactivity of thymine with FcCDI was higher than that of guanine, but independent of their location on the DNA sequence. After a single stranded DNA was treated with FcCDI, resulting ODN could be detected electrochemically by a redox signal deriving from the ferrocenyl moiety with a DNA probe-immobilized electrode. These results suggest that the labeling of DNA with FcCDI can be applied to rapid analysis of electrochemical genosensors.
Mukumoto
Kosuke
K
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812-8581, Japan.
Nojima
Takahiko
T
Takenaka
Shigeori
S
D002234:Carbodiimides / Q000138:chemical synthesis* / Q000737:chemistry; D018736:DNA Adducts; D015342:DNA Probes; D004277:DNA, Single-Stranded / Q000032:analysis*; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D000075163:Metallocenes; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D013941:Thymine
D002234:Carbodiimides; D018736:DNA Adducts; D015342:DNA Probes; D004277:DNA, Single-Stranded; D005296:Ferrous Compounds; D000075163:Metallocenes; D009838:Oligodeoxyribonucleotides; D013941:Thymine; C004998:ferrocene