Effect of "Profetal" on differentiation and functional activity of human mononuclear leukocytes.
The effect of "Profetal", a preparation containing human alpha-fetoprotein, on proliferative activity and differentiation of mononuclear leukocytes isolated from donor peripheral blood was studied in cultures with serum-free media. The results suggest that human alpha-fetoprotein in effective doses stimulates proliferation and blast transformation of cultured peripheral blood mononuclear leukocytes with the formation of CD34+/CD45+ hemopoietic precursor cells. This phenomenon of ex vivo generation of hemopoietic precursor cells using human alpha-fetoprotein can be important for clinical oncology, specifically, for the development of adaptive cell therapies.
Lebedinskaya
O V
OV
Perm State Medical Academy, Ministry of Health of the Russian Federation, Perm. lebedinska@mail.ru
Velizheva
N P
NP
Donenko
F V
FV
Chereshnev
V A
VA
Rodionov
S Yu
SY
Akhmatova
N K
NK
Shubina
I Zh
IZh
Lebedinskaya
E A
EA
Kiselevskii
M V
MV
D018952:Antigens, CD34 / Q000096:biosynthesis; D002454:Cell Differentiation; D049109:Cell Proliferation; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D008962:Models, Theoretical; D010641:Phenotype; D013234:Stem Cells / Q000378:metabolism; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000378:metabolism / Q000494:pharmacology*
D018952:Antigens, CD34; D016895:Culture Media, Serum-Free; D000509:alpha-Fetoproteins; D017493:Leukocyte Common Antigens