Detection of a single DNA base-pair mismatch using an anthracene-tagged fluorescent probe.
A novel anthracene-tagged oligonucleotide can discriminate between a fully-matched DNA target sequence and one with a single mismatching base-pair through a remarkable difference in fluorescence emission intensity upon duplex formation.
Moran
Nina
N
School of Chemistry, University of Birmingham, Edgbaston, Birmingham, UK.
Bassani
Dario M
DM
Desvergne
Jean-Pierre
JP
Keiper
Sonja
S
Lowden
Philip A S
PA
Vyle
Joseph S
JS
Tucker
James H R
JH
D000873:Anthracenes / Q000737:chemistry*; D020137:Base Pair Mismatch / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D000873:Anthracenes; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D004247:DNA