title: Association of ceramides in human plasma with risk factors of atherosclerosis.
abstract: Atherosclerosis is a multifactorial disorder. Recent studies indicate that the plasma level of sphingomyelin, which yields ceramide, correlates with the risk of coronary heart disease. Therefore, ceramide, a well-known lipid causing apoptosis in various cell types, may contribute to atherogenesis. We examined the relationship between ceramide concentration and risk factors of atherosclerosis in normal human plasma using electrospray tandem mass spectrometry (LC-MS/MS). Major ceramides in human plasma were C24:0 and C24:1. The ceramide concentration showed a significant positive correlation with total cholesterol (TC) and triglycerides (TG). In addition, plasma ceramide level increased drastically at a high level of LDL cholesterol (more than 170 mg/dL). Our previous studies demonstrated that the sum of fragmented and conjugated apolipoprotein B-100 proteins (B-ox), which were products of a radical reaction of LDL as well as plasma, was a reliable index of atherosclerosis. B-ox showed a significant positive correlation with the plasma ceramide level. Based on these results, we propose that the ceramide level in human plasma is a risk factor at the early stages of atherosclerosis.
authors.affiliation: Department of Food Science and Nutrition, Nara Women's University, Nara 630-8506, Japan.
authors.forename: Ikuyo
authors.initials: I
authors.lastname: Ichi
authors.forename: Kayoko
authors.initials: K
authors.lastname: Nakahara
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Miyashita
authors.forename: Atsuko
authors.initials: A
authors.lastname: Hidaka
authors.forename: Sahoko
authors.initials: S
authors.lastname: Kutsukake
authors.forename: Kana
authors.initials: K
authors.lastname: Inoue
authors.forename: Taro
authors.initials: T
authors.lastname: Maruyama
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Miwa
authors.forename: Mariko
authors.initials: M
authors.lastname: Harada-Shiba
authors.forename: Motoo
authors.initials: M
authors.lastname: Tsushima
authors.forename: Shosuke
authors.initials: S
authors.lastname: Kojo
mesh_terms: D053299:Apolipoprotein B-100 / Q000097:blood; D050197:Atherosclerosis / Q000097:blood* / Q000453:epidemiology; D002518:Ceramides / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008403:Mass Screening; D008875:Middle Aged; D011237:Predictive Value of Tests; D012307:Risk Factors; D021241:Spectrometry, Mass, Electrospray Ionization; D014280:Triglycerides / Q000097:blood
chemical_list: D053299:Apolipoprotein B-100; D002518:Ceramides; D008078:Cholesterol, LDL; D014280:Triglycerides; D002784:Cholesterol