Stabilization of the left-handed Z-DNA with monoamines and polyamines.
It is known that the crystal structure of d(CG)3 become left-handed Z-DNA under high salt concentration and various polyamines stabilize the Z-DNA structure. We have structurally investigated how polyamines stabilize the Z-DNA by the X-ray crystallographic analysis of d(CG)3-polyamine cocrystals. In this study, we determined the Z-DNA structures with di- to pentavalent polyamines at high resolution. Comparison with the structures revealed that the valence of polyamine determined number of the polyamine(s) and metal ion(s) chelating to the Z-DNA. Furthermore, we succeeded in crystallizing d(CG)3-monoamine (methylamine and ethylamine) complexes.
Tsukamoto
Koji
K
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Odoko
Mamiko
M
Ohishi
Hirofumi
H
Hiyama
Yoichi
Y
Maezaki
Naoyoshi
N
Okabe
Nobuo
N
Tanaka
Tetsuaki
T
Ishida
Toshimasa
T
D000588:Amines / Q000737:chemistry*; D018360:Crystallography, X-Ray; D043542:DNA, Z-Form / Q000737:chemistry*; D018900:Dinucleotide Repeats; D005021:Ethylamines / Q000737:chemistry; D008744:Methylamines / Q000737:chemistry; D008958:Models, Molecular; D011073:Polyamines / Q000737:chemistry*
D000588:Amines; D043542:DNA, Z-Form; D005021:Ethylamines; D008744:Methylamines; D011073:Polyamines; C027451:methylamine; C041564:ethylamine