Suitable restriction enzymes for pulsed-field gel electrophoresis analysis of Candida tropicalis.
This study was designed to pursue the identification of optimal restriction enzyme for pulsed-field gel electrophoresis (PFGE) analysis of Candida tropicalis. Twelve restriction enzymes were tested on C. tropicalis strains. Our results indicate that BssHII, NaeI, and RsrII were useful enzymes for PFGE analysis, and NaeI was the best for PFGE analysis of C. tropicalis isolates.
Chou
Hsiao-Hui
HH
Mycotic Diseases Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taipei 11561, Taiwan.
Chiou
Chien-Shun
CS
Chen
Kuo-Wei
KW
Li
Shu-Ying
SY
D041022:Candida tropicalis / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D004271:DNA, Fungal / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005838:Genotype; D006801:Humans; D016533:Mycological Typing Techniques
D004271:DNA, Fungal; C060630:endodeoxyribonuclease NaeI; C043032:endodeoxyribonuclease RsrII; D015252:Deoxyribonucleases, Type II Site-Specific