title: Intestinal bacteria activate estrogenic effect of main constituents puerarin and daidzin of Pueraria thunbergiana.
abstract: To understand the relationship between the metabolites and estrogenic activity of the main isoflavones puerarin and daidzin of the rhizome of Pueraria thunbergiana (PT, family Leguminosae), PT and its isoflavones were transformed by human intestinal bacteria and their estrogenic effects were investigated. All human fecal specimens hydrolyzed puerarin and daidzin to daidzein, but their hydrolyzing activities varied depending on the individuals. All intestinal bacteria isolated from human also hydrolyzed daidzin to daidzein, but a few bacteria transformed puerarin to daidzein. When the estrogenic effect of PT, puerarin and daidzin was compared with those of their metabolites, the metabolites more potently increased proliferation of MCF-7 cells than PT, puerarin and daidzin. The metabolite daidzein also potently increased estrogen-response c-fos mRNA and PR protein expressions. These findings suggest that intestinal bacteria, which can hydrolyze puerarin and/or daidzin, may activate a potent estrogenic activity of PT.
authors.affiliation: College of Pharmacy, Kyung Hee University, Seoul, Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Park
authors.forename: Jieun
authors.initials: J
authors.lastname: Shin
authors.forename: Eun-Ah
authors.initials: EA
authors.lastname: Bae
authors.forename: Young-Chul
authors.initials: YC
authors.lastname: Lee
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
mesh_terms: D001419:Bacteria / Q000145:classification; D018407:Bacterial Physiological Phenomena; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007529:Isoflavones / Q000494:pharmacology*; D048789:Phytoestrogens / Q000494:pharmacology*; D029908:Pueraria / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D007529:Isoflavones; D048789:Phytoestrogens; C013908:daidzin; C033607:puerarin