Preparative isolation and purification of harpagoside from Scrophularia ningpoensis hemsley by high-speed counter-current chromatography.
The bioactive component harpagoside was successfully separated from the crude extract of Scrophularia ningpoensis Hemsley by one-step purification using high-speed counter-current chromatography (HSCCC). A two-phase solvent system containing n-butanol:ethyl acetate:water (1:9:10) was selected following consideration of the partition coefficient of the target compound. A 276 mg quantity of the crude extract was loaded onto a 250 mL HSCCC column and yielded 11 mg harpagoside at over 97% purity. The chemical structure of harpagoside was determined by HPLC-ESI/MS and 1H-NMR.
Tong
Shengqiang
S
College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.
Yan
Jizhong
J
Lou
Jianzhong
J
D003377:Countercurrent Distribution / Q000379:methods*; D006027:Glycosides / Q000737:chemistry*; D015394:Molecular Structure; D011714:Pyrans / Q000737:chemistry*; D032266:Scrophularia / Q000737:chemistry*
D006027:Glycosides; D011714:Pyrans; C033249:harpagoside