title: Evaluation of respiratory model employing conventional NIH mice to access the immunity induced by cellular and acellular pertussis vaccines.
abstract: The increasing number of pertussis cases reported on the last twenty years and the existence of new acellular vaccines reinforce the need of research for experimental models to assure the quality of available pertussis vaccines. In this study, allotments of whole-cell and acellular pertussis vaccines were tested through the Intranasal Challenge Model (INM) using conventional NIH mice. The results have been compared to those achieved by the "Gold standard" Intracerebral Challenge Model (ICM). In contrast to ICM, INM results did not show intralaboratorial variations. Statistical analysis by Anova and Ancova tests revealed that the INM presented reproducibility and allowed identification and separation of different products, including three-component and four-component accellular pertussis vaccines. INM revealed differences between pertussis vaccines. INM provides lower distress to the mice allowing the reduction of mice number including the possibility of using conventional mice (less expensive) under non-aseptic environment. Thus, INM may be used as an alternative method of verifying the consistence of allotment production, including acellular pertussis vaccines.
authors.affiliation: Laboratório de Vacinas Bacterianas, Departamento de Imunologia, Instituto Nacional de Controle de Qualidade em Saúde, Fiocruz, 21040-900 Rio de Janeiro, RJ, Brazil. alexandre.dias@incqs.fiocruz.br
authors.forename: Alexandre Alves de Souza de Oliveira
authors.initials: AA
authors.lastname: Dias
authors.forename: Maria Aparecida Affonso
authors.initials: MA
authors.lastname: Boller
authors.forename: Lúcia Maria Correa
authors.initials: LM
authors.lastname: Werneck
authors.forename: Raphael
authors.initials: R
authors.lastname: Hirata Junior
authors.forename: Ana Luíza
authors.initials: AL
authors.lastname: Mattos-Guaraldi
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001886:Bordetella pertussis / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D007111:Immunity, Cellular; D008297:Male; D051379:Mice; D010567:Pertussis Vaccine / Q000009:adverse effects / Q000276:immunology* / Q000592:standards; D015203:Reproducibility of Results; D013997:Time Factors; D022282:Vaccines, Acellular / Q000009:adverse effects / Q000276:immunology / Q000592:standards; D014614:Vaccines, Synthetic / Q000009:adverse effects / Q000276:immunology / Q000592:standards; D014917:Whooping Cough / Q000276:immunology* / Q000517:prevention & control
chemical_list: D010567:Pertussis Vaccine; D022282:Vaccines, Acellular; D014614:Vaccines, Synthetic