[Construction of mutant myocilin plasmid and its expression in human trabecular meshwork cells].
OBJECTIVE
To establish the mutant Myocilin gene plasmid in order to study the function of Myocilin.


METHODS
The mutant site was induced by site-directed mutagenesis. This plasmid were transfected into human trabecular meshwork cells (HTM cells) by cationic liposomes and the expression of Myocilin was examined by RT-PCR and Western blot analysis.


RESULTS
The mutant plasmid were correctly constructed. Myocilin was efficiently expressed in HTM cells transfected with this plasmid.


CONCLUSIONS
The constructed eukaryote expression plasmid pcDNA-MYOC-P370L could express Myocilin in HTM cells in vitro.
Wang
Lina
L
Key Laboratory of Ophthalmology of Ministry of Education and Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Ge
Jian
J
Liu
Bingqian
B
Song
Ge
G
Hou
Fei
F
D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D005902:Glaucoma, Open-Angle / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D017354:Point Mutation; D014129:Trabecular Meshwork / Q000166:cytology*; D014162:Transfection; D000099179:Myocilin
D003598:Cytoskeletal Proteins; D005136:Eye Proteins; D006023:Glycoproteins; D000099179:Myocilin