Enhancement of RNA cleavage activity of 10-23 DNAzyme by covalently introduced intercalator.
By introducing an intercalator through D-threoninol to the 10-23 DNAzyme at the junction between its catalytic loop and the binding arm, the RNA cleavage activity was greatly improved.
Asanuma
Hiroyuki
H
Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan. asanuma@mol.nagoya-u.ac.jp
Hayashi
Hiroyuki
H
Zhao
Jing
J
Liang
Xingguo
X
Yamazawa
Akira
A
Kuramochi
Takeshi
T
Matsunaga
Daijiro
D
Aiba
Yuichiro
Y
Kashida
Hiromu
H
Komiyama
Makoto
M
D000880:Anthraquinones / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry / Q000528:radiation effects; D001483:Base Sequence; D002384:Catalysis / Q000528:radiation effects; D021881:DNA, Catalytic / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D007536:Isomerism; D007700:Kinetics; D008027:Light; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000737:chemistry; D010777:Photochemistry; D011721:Pyrenes / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D013912:Threonine / Q000031:analogs & derivatives / Q000737:chemistry; D014466:Ultraviolet Rays
D000880:Anthraquinones; D001391:Azo Compounds; D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D007364:Intercalating Agents; D009843:Oligoribonucleotides; D011721:Pyrenes; C402582:RNA-cleaving DNA 10-23; D013912:Threonine; D012313:RNA; C009850:azobenzene