Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation.
Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.
Sherwood
Victoria
V
Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonnington Campus, Loughborough LE12 5RD, UK.
Burgert
Hans-Gerhard
HG
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
Chen
Yun-Hsiang
YH
School of Biology, University of St Andrews, Fife KY16 9TS, UK.
Sanghera
Sandeep
S
Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonnington Campus, Loughborough LE12 5RD, UK.
Katafigiotis
Socrates
S
Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonnington Campus, Loughborough LE12 5RD, UK.
Randall
Richard E
RE
School of Biology, University of St Andrews, Fife KY16 9TS, UK.
Connerton
Ian
I
Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonnington Campus, Loughborough LE12 5RD, UK.
Mellits
Kenneth H
KH
Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonnington Campus, Loughborough LE12 5RD, UK.
D000260:Adenoviruses, Human / Q000254:growth & development* / Q000502:physiology; D002460:Cell Line; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D050794:STAT1 Transcription Factor / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication / Q000187:drug effects*
D050794:STAT1 Transcription Factor; D007372:Interferons