Schlegelella aquatica sp. nov., a novel thermophilic bacterium isolated from a hot spring.
A moderately thermophilic bacterial strain designated wcf1(T), isolated from a hot spring located in the Tainan area, southern Taiwan, was characterized using a polyphasic approach. The cells were Gram-negative, non-pigmented, rod-shaped, non-spore-forming and motile. Phylogenetic analysis using 16S rRNA gene sequences showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus Schlegelella; its only close neighbour was the type strain of Schlegelella thermodepolymerans, K14(T) (97.8 %). The isolate was clearly distinguishable from other strains using phylogenetic analysis, DNA-DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristics. It was evident from the genotypic and phenotypic data that strain wcf1(T) represents a novel species in the genus Schlegelella, for which the name Schlegelella aquatica sp. nov. is proposed, with the type strain wcf1(T) (=BCRC 17557(T)=LMG 23380(T)).
Chou
Yi-Ju
YJ
Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan.
Sheu
Shih-Yi
SY
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan.
Sheu
Der-Shyan
DS
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan.
Wang
Jih-Terng
JT
Department of Biotechnology, Tajen University, Pingtung, Taiwan.
Chen
Wen-Ming
WM
Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan.
D015373:Bacterial Typing Techniques; D020562:Betaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013624:Taiwan
D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S