title: [Laccase of the lignolytic fungus Trametes hirsuta: purification and characterization of the enzyme, and cloning and primary structure of the gene].
abstract: The main physicochemical characteristics of the major isoform of the laccase secreted by the fungu, Trametes hirsuta 072 were studied. The enzyme belongs to the group of high redox potential laccases (E(T1) = 790 +/- 5), and it oxidizes with high efficiency various substrates of phenolic nature. The gene of this isoform was cloned, and its nucleotide sequence was determined. The length of the complete gene is 2134 bp. It comprises 11 exons and 10 introns. Analysis of the amino acid sequence of T. hirsuta 072 laccase demonstrated a high homology (to 96.9%) to the other laccases secreted by fungi of the genus Trametes.
authors.forename: D V
authors.initials: DV
authors.lastname: Rebrikov
authors.forename: D N
authors.initials: DN
authors.lastname: Rebrikov
authors.forename: E V
authors.initials: EV
authors.lastname: Stepanova
authors.forename: O V
authors.initials: OV
authors.lastname: Koroleva
authors.forename: Zh I
authors.initials: ZhI
authors.lastname: Budarina
authors.forename: M V
authors.initials: MV
authors.lastname: Zakharova
authors.forename: T V
authors.initials: TV
authors.lastname: Iurkova
authors.forename: A S
authors.initials: AS
authors.lastname: Solonin
authors.forename: O V
authors.initials: OV
authors.lastname: Belova
authors.forename: Z A
authors.initials: ZA
authors.lastname: Pozhidaeva
authors.forename: A A
authors.initials: AA
authors.lastname: Leont'evskiĭ
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D005800:Genes, Fungal; D042845:Laccase / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D020072:Polyporales / Q000201:enzymology*; D017422:Sequence Analysis, DNA
chemical_list: D005656:Fungal Proteins; D042845:Laccase