Toxoid flocculation assay by laser light-scattering.
Internationally accepted designations of antigen content for toxoid vaccines are provided by the WHO in Lf (limes flocculationis) units, based on the formation of antigen-antibody complexes. The current assay method for Lf determination involves observation of the complexes by eye, making the development of a more objective system highly desirable. Here we report a novel detection system using a laser light-scattering platelet aggregometer. The system was highly reproducible and more objective than the current method. Only three sets of duplicate data were sufficient for statistically significant determination of toxoid Lf by parabolic regression.
Iwaki
Masaaki
M
Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011 Japan. miwaki@nih.go.jp
Horiuchi
Yoshinobu
Y
Komiya
Takako
T
Fukuda
Tadashi
T
Arakawa
Yoshichika
Y
Takahashi
Motohide
M
D000936:Antigen-Antibody Complex / Q000032:analysis*; D004166:Diphtheria Antitoxin / Q000276:immunology; D004168:Diphtheria Toxoid / Q000032:analysis / Q000276:immunology; D005428:Flocculation Tests / Q000379:methods; D007834:Lasers; D008027:Light; D010316:Particle Size; D012044:Regression Analysis; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D013743:Tetanus Antitoxin / Q000276:immunology; D013745:Tetanus Toxoid / Q000032:analysis / Q000276:immunology; D014121:Toxoids / Q000032:analysis* / Q000276:immunology
D000936:Antigen-Antibody Complex; D004166:Diphtheria Antitoxin; D004168:Diphtheria Toxoid; D013743:Tetanus Antitoxin; D013745:Tetanus Toxoid; D014121:Toxoids