Structure, stability and application to functional DNA/RNA of unique quadruplex.
We have developed a direct and unambiguous method for discrimination of intra- and intermolecular hydrogen bonds in a symmetric multimer. The method was applied to a symmetric dimer of d(GGAGGAGGAGGA) (GGA 12-mer) and has provided decisive information on its multimeric architecture. Then, the values for scalar couplings across hydrogen bonds for G:G and G:A base pairs in the G(:A):G(:A):G(:A):G heptad formed by GGA 12-mer were determined. This determination has provided an insight into the stability of the heptad. Moreover, GGA 12-mer has been incorporated into functional DNA, and unique structural features of GGA 12-mer have been successfully utilized to regulate the activity of functional DNA.
Niyada
Emi
E
Department of Environment and Natural Sciences, Graduate School of Environment and Information Sciences, Yokohama National University, 79-7 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.
Sotoya
Hidetsugu
H
Matsugami
Akimasa
A
Ouhashi
Kiyoshi
K
Uesugi
Seiichi
S
Katahira
Masato
M
D020029:Base Pairing; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D006860:Hydrogen Bonding; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D009692:Nucleic Acid Heteroduplexes; D009838:Oligodeoxyribonucleotides; D012313:RNA; D004247:DNA