Adipocytes and preadipocytes promote the proliferation of colon cancer cells in vitro.
Obesity, a risk factor for colon cancer, is associated with elevated serum levels of leptin, a protein produced by adipocytes. The aim of the present study was to clarify the effects of adipose tissue on colon cancer proliferation by using cultured cell lines. To achieve this, colon cancer cells (CACO-2, T84, and HT29) were cocultured with adipose tissue, isolated mature adipocytes, and isolated preadipocytes in a three-dimensional collagen gel culture system. The adipocytes and preadipocytes used were isolated from C57BL/6J and leptin-deficient ob/ob mice. Proliferation of the cancer cells was evaluated by nuclear bromodeoxyuridine uptake. The adipose tissue, mature adipocytes, and preadipocytes isolated from C57BL/6J mice significantly increased the proliferation of the colon cancer cells. This trophic effect of mature adipocytes on the cancer cell lines was observed only for cells from lean littermates and not for those from ob/ob mice. In contrast, the trophic effect of preadipocytes was not abolished in ob/ob mice, and this finding was supported by the result that leptin had a trophic effect on cancer cells. In conclusion, adipocytes were able to enhance the proliferation of colon cancer cells in vitro, partly via leptin, suggesting that adipose tissues, including mature adipocytes and preadipocytes, may promote the growth of colorectal cancer.
Amemori
Sadahiro
S
Department of Internal Medicine, Saga Medical School, Saga, Japan.
Ootani
Akifumi
A
Aoki
Shigehisa
S
Fujise
Takehiro
T
Shimoda
Ryo
R
Kakimoto
Takashi
T
Shiraishi
Ryosuke
R
Sakata
Yasuhisa
Y
Tsunada
Seji
S
Iwakiri
Ryuichi
R
Fujimoto
Kazuma
K
D052438:Adipocytes, White / Q000166:cytology* / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000818:Animals; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018920:Coculture Techniques / Q000379:methods; D003094:Collagen / Q000737:chemistry; D003110:Colonic Neoplasms / Q000473:pathology; D005782:Gels; D019073:HT29 Cells; D006801:Humans; D020738:Leptin / Q000172:deficiency / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008820:Mice, Obese; D011956:Receptors, Cell Surface / Q000032:analysis; D054411:Receptors, Leptin; D017154:Stromal Cells / Q000473:pathology
D005782:Gels; D020738:Leptin; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D003094:Collagen