Electrochemical detection of pathogen infection using cell chip.
The immobilized cell using self-assembled synthetic oligopeptide was applied to the electrochemical detection of pathogen infection. Thin films based on cysteine-terminated synthetic oligopeptides were fabricated for the immobilization of HeLa cell on gold (Au) substrate. Layer formation and immobilization of the cell were investigated with surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS). Experimental results showed that the thin film of cysteine-terminated synthetic oligopeptide was successfully fabricated and it could be applied for the immobilization of HeLa cells. The adhered living cell was exposed to E. coli O157:H7, which induced the change of SPR angle and electrochemical impedance signal. The proposed cell immobilization method using self-assembly technique can be applied to construct the cell microarray for on-site pathogen monitoring.
Choi
J W
JW
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Sinsu-Dong, Mapo-Gu, Seoul, 121-742, South Korea.
Lee
W
W
Lee
D B
DB
Park
C H
CH
Kim
J S
JS
Jang
Y H
YH
Kim
Y
Y
D002277:Carcinoma; D018914:Cells, Immobilized; D004563:Electrochemistry / Q000379:methods*; D004784:Environmental Monitoring; D004847:Epithelial Cells / Q000276:immunology*; D019453:Escherichia coli O157; D007723:Korea; D009842:Oligopeptides
D009842:Oligopeptides