Molecular basis of succinylcholine sensitivity in a prairie Hutterite kindred and genetic characterization of the region containing the BCHE gene.
The tetrameric glycoprotein butyrylcholinesterase (BChE; EC 3.1.1.8) is one of two enzymes that hydrolyze choline esters. The controlling gene (BCHE) is comprised of four coding exons and is located on chromosome 3q26. Based on BChE activity measurements in the presence and absence of dibucaine, usual (designated U) and atypical (designated A) gene products have been distinguished. Homozygotes for the A gene product are at risk for prolonged apnea following exposure to the surgical anesthetics succinylcholine or mivacurium. In this report, we detail biochemical and molecular investigations of succinylcholine sensitivity in a prairie Hutterite kindred. Our results establish that BChE activities in the family members are impacted by two distinct BCHE mutations, namely, c.209A>G p. D70G and c.1615G>A p. A539T. However, homozygotes for the c.209A>G mutation (i.e., atypical or A) are the only individuals whose BChE activity could lead to adverse reactions to succinylcholine. Interestingly, haplotype analysis of the chromosomal region containing BCHE indicates that the c.209A>G mutation is carried on a unique haplotype, suggesting that it was likely introduced into the population only once. Conversely, the c.1615G>A mutation is carried on various haplotypes and was likely introduced into the population more than once.
Zelinski
T
T
Department of Pediatrics and Child Health, Room P009, Pathology Building, 770 Bannatyne Avenue, Faculty of Medicine, University of Manitoba, Winnipeg, Man., Canada R3E . zelinski@cc.umanitoba.ca
Coghlan
G
G
Mauthe
J
J
Triggs-Raine
B
B
D001049:Apnea / Q000235:genetics; D002091:Butyrylcholinesterase / Q000097:blood / Q000187:drug effects / Q000235:genetics*; D002170:Canada; D003992:Dibucaine / Q000494:pharmacology; D005006:Ethnicity; D005260:Female; D005828:Genetics, Population; D006801:Humans; D008297:Male; D009154:Mutation; D013390:Succinylcholine / Q000009:adverse effects*; D044465:White People / Q000235:genetics
D002091:Butyrylcholinesterase; D013390:Succinylcholine; D003992:Dibucaine