Electrochemical detection of DNase I activity.
DNase I in one microl of the water could quantitate electrochemically with the detection limit of 0.01 units (ca. 20 pg) by using the ferrocenyl oligonucleotide-immobilized electrode prepared by thiolated oligonucleotide and ferrocenyl carbodiimide as a simple labeling reagent of redox unit.
Fujita
Katsuya
K
Department of Materials Science, Faculty of Engineering, Kyushu Institute of Technology, 1-1 Sensui-cyo, Tobata-ku, Kitakyushu-shi, Fukuoka 804-8550, Japan.
Kanazawa
Masanori
M
Mukumoto
Kosuke
K
Nojima
Takahiko
T
Sato
Shinobu
S
Kondo
Hiroki
H
Waki
Michinori
M
Takenaka
Shigeori
S
D002234:Carbodiimides / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D010084:Oxidation-Reduction; D013873:Thionucleotides / Q000737:chemistry
D002234:Carbodiimides; D005296:Ferrous Compounds; D009841:Oligonucleotides; D013873:Thionucleotides; C517870:ferrocenyl carbodiimide; D003850:Deoxyribonuclease I