title: IADS, a decomposition product of DIDS activates a cation conductance in Xenopus oocytes and human erythrocytes: new compound for the diagnosis of cystic fibrosis.
abstract: DIDS (4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid) is a commonly used blocker of plasma membrane anion channels and transporters. We observed that DIDS undergoes decomposition while stored in DMSO (dimethyl sulfoxide) forming a biologically active compound. One decomposition product, called IADS, was identified and synthesized. Voltage-clamp and patch clamp experiments on Xenopus laevis oocytes and human erythrocytes revealed that IADS is able to activate a plasma membrane cation conductance in both cell types. Furthermore, we found that IADS induces hemolysis in red blood cells of healthy donors but fails to hemolyze erythrocytes of donors with cystic fibrosis. Thus, IADS stimulated activation of a cation conductance could form the basis for a novel diagnostic test of cystic fibrosis.
authors.affiliation: Institute of Physiology II, University of Münster, Münster, Germany.
authors.forename: Astrid
authors.initials: A
authors.lastname: Stumpf
authors.forename: Joana
authors.initials: J
authors.lastname: Almaca
authors.forename: Karl
authors.initials: K
authors.lastname: Kunzelmann
authors.forename: Kerstin
authors.initials: K
authors.lastname: Wenners-Epping
authors.forename: Stephan M
authors.initials: SM
authors.lastname: Huber
authors.forename: Johannes
authors.initials: J
authors.lastname: Haberle
authors.forename: Sabine
authors.initials: S
authors.lastname: Falk
authors.forename: Angelika
authors.initials: A
authors.lastname: Duebbers
authors.forename: Mike
authors.initials: M
authors.lastname: Walte
authors.forename: Hans
authors.initials: H
authors.lastname: Oberleithner
authors.forename: Hermann
authors.initials: H
authors.lastname: Schillers
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002412:Cations / Q000378:metabolism; D003550:Cystic Fibrosis / Q000175:diagnosis*; D004594:Electrophysiology; D004912:Erythrocytes / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006461:Hemolysis; D006801:Humans; D017136:Ion Transport / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D014981:Xenopus
chemical_list: C517099:4-isothiocyanato-4'-amino-stilbene-2,2'-disulfonic acid; D002412:Cations; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid