The development of crystallization and X-ray crystallography method of long stem DNA.
We clarified stabilization mechanism of left-handed Z-DNA by X-ray crystal structure analysis. I was able to achieve it by burying big minor groove of Z-DNA because metal ion and polyamine connected it to minor groove of Z-DNA. On that occasion I understood that an electric charge and length of polyamine gave big influence to a combination brain very much. I succeeded in crystallization of Z-DNA of a long chain, X-ray crystal structure analysis by examining these combination ability.
Ohishi
Hirofumi
H
Osaka University of Pharmaceutical Sciences 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Tukamoto
Koji
K
Odoko
Mamiko
M
Grzeskowiak
Kazimierz
K
Maezaki
Naoyoshi
N
Okabe
Nobuo
N
Tanaka
Tetsuaki
T
Ishida
Toshimasa
T
D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray; D043542:DNA, Z-Form / Q000737:chemistry*; D008670:Metals / Q000737:chemistry; D009690:Nucleic Acid Conformation; D011073:Polyamines / Q000737:chemistry
D043542:DNA, Z-Form; D008670:Metals; D011073:Polyamines