title: Identification of F-box proteins that are involved in resistance to methylmercury in Saccharomyces cerevisiae.
abstract: We searched for F-box proteins that might be related to the mechanism that protects Saccharomyces cerevisiae against the toxic effects of methylmercury. We found that overexpression of Hrt3 and of Ylr224w rendered yeast cells resistant to methylmercury. Yeast cells that overexpressed Hrt3 and Ylr224w were barely resistant to methylmercury in the presence of a proteasome inhibitor. Our results suggest the existence of some protein(s) that enhances the toxicity of methylmercury in yeast cells and, also, that overexpression of Hrt3 or Ylr224w can confer resistance to methylmercury by enhancing the polyubiquitination of this protein(s) and its degradation in proteasomes.
authors.affiliation: Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan.
authors.forename: Gi-Wook
authors.initials: GW
authors.lastname: Hwang
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Ishida
authors.forename: Akira
authors.initials: A
authors.lastname: Naganuma
mesh_terms: D025141:Drug Resistance, Fungal / Q000187:drug effects*; D044782:F-Box Motifs; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D008767:Methylmercury Compounds / Q000633:toxicity*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D061988:Proteasome Inhibitors; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D044783:F-Box Proteins; D008767:Methylmercury Compounds; D061988:Proteasome Inhibitors; D046988:Proteasome Endopeptidase Complex