title: Mechanism of PNA transport to the nuclear compartment.
abstract: We evaluated the nuclear uptake of fluorescently labeled peptide nucleic acids and measured the binding of unlabeled peptide nucleic acids (PNAs) to the endogenous HER-2/neu promotor in digitonin-permeabilized SK-BR-3 cells. Fluorescently labeled PNAs readily enter the nucleus of digitonin-permeabilized cells, and binding to the chromosomal target sequence was detected with a bis-PNA. Nuclear uptake and target sequence binding were inhibited by N-ethylmaleimide (NEM) and GTPgammaS. We conclude that PNAs are transported into the nucleus through an energy-dependent process involving the nuclear pore complex.
authors.affiliation: Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724-5024, USA.
authors.forename: Lenka
authors.initials: L
authors.lastname: Stankova
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Ziemba
authors.forename: Zhanna V
authors.initials: ZV
authors.lastname: Zhilina
authors.forename: Scot W
authors.initials: SW
authors.lastname: Ebbinghaus
mesh_terms: D021581:Active Transport, Cell Nucleus; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002875:Chromosomes / Q000378:metabolism; D005456:Fluorescent Dyes; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006801:Humans; D022022:Nuclear Pore; D020135:Peptide Nucleic Acids / Q000493:pharmacokinetics*; D011401:Promoter Regions, Genetic; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics
chemical_list: D005456:Fluorescent Dyes; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D020135:Peptide Nucleic Acids; D018719:Erb-b2 Receptor Tyrosine Kinases