SNPs typing by base-discriminating fluorescence DNA probe.
For the development of a novel SNP typing method using BDF (base-discriminating fluorescent) nucleosides in biological samples, we examined the detection of the single base alteration in BRCA1 gene with PCR products amplified by an asymmetric PCR. A combination of PyU- and PyC-containing BDF probes clearly facilitates the discrimination of not only A/G homozygous samples but also heterozygous samples. The present SNP typing method with BDF probes is a very powerful homogeneous assay that does not require a special device or time-consuming steps.
Okamoto
Akimitsu
A
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan.
Tainaka
Kazuki
K
Ochi
Yuji
Y
Saito
Isao
I
D015342:DNA Probes / Q000737:chemistry*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000737:chemistry; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D019398:Genes, BRCA1; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011741:Pyrimidine Nucleosides / Q000737:chemistry
C520209:4-N-(N,N-dimethylaminomethylidenyl)-5-(3-(1-pyrenecarboxamido)propynyl)-5'-O-(4,4-dimethoxytrityl)-2'-deoxycytidine; C520208:5-(3-(1-pyrenecarboxamido)propynyl)-2'-deoxyuridine; D015342:DNA Probes; D005456:Fluorescent Dyes; D011741:Pyrimidine Nucleosides; D003841:Deoxycytidine; D003857:Deoxyuridine