title: Fast RPLC-UV method on short sub-two microns particles packed column for the assay of tenoxicam in plasma samples.
abstract: An extraction-less sample preparation technique followed by a RPLC-UV method on sub-two microns particles packed short column were used for the assay of tenoxicam in plasma samples. Protein precipitation was made by means of trichloroacetic acid addition. Supernatant was injected to the chromatographic column without any further pH adjustment. The mobile phase consisted in a mixture of acetonitrile and aqueous 0.1% phosphoric acid, at 2 mL/min flow rate and gradient elution. The Zorbax SB-C18 column (50 mm length, 4.6 mm internal diameter and 1.8 microm particle size) was thermostated at 60 degrees C. The mobile phase gradient composition program allowed separation of tenoxicam and piroxicam (internal standard), column clean-up and re-equilibration within 4 min. UV detection was achieved at 368+/-10 nm. The method is characterized by a low limit of quantitation of 25 ng/mL for tenoxicam, with a linearity interval up to 5500 ng/mL. The use of a low volume detection cell and detector high frequency data acquisition rate produced high precision and accuracy through a whole bioequivalence study of tenoxicam in two commercially available tablet formulations, after a single oral administration dose. Full method validation is presented. The high throughput characteristic of the proposed method allowed full validation and bioanalytical study completion within a 96 h period.
authors.affiliation: LaborMed Pharma S.A., Splaiul Independentei 319E, Bucharest 060044, Romania.
authors.forename: Iulia
authors.initials: I
authors.lastname: Sora
authors.forename: Toma
authors.initials: T
authors.lastname: Galaon
authors.forename: Stefan
authors.initials: S
authors.lastname: Udrescu
authors.forename: Jean
authors.initials: J
authors.lastname: Negru
authors.forename: Victor
authors.initials: V
authors.lastname: David
authors.forename: Andrei
authors.initials: A
authors.lastname: Medvedovici
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D001681:Biological Assay; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D010894:Piroxicam / Q000031:analogs & derivatives* / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013997:Time Factors
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010894:Piroxicam; C032801:tenoxicam