title: Influence of glass and polymer coatings on CHO cell morphology and adhesion.
abstract: Successful development of cell-on-chip microsystems where living cells are deposited and grown in microfabricated structures is highly dependent on the control of cell/substrate interactions. In this study, several materials of interest were tested for CHO cell growth and morphology: (i) glass, fibronectin-, poly-L-lysine- and 3-aminopropyltriethoxysilane (APTES)--treated glass and UV/O(3)-modified PDMS coating on glass as well as (ii) silicon, poly-L-lysine-, APTES-, O(2) plasma-treated and oxide-coated silicon. In addition, we quantitatively characterized cell adhesion to these substrates using a radial flow detachment assay. Lack of correlation between cell adhesion and cell morphology was systematically observed for all substrates. In particular, we show that PDMS coatings on glass can be finely tuned by UV/O(3) treatment to enhance cell adhesion and induce elongated morphology. Moreover, we observed a low shear stress cell detachment mechanism on silicon oxide coatings on silicon wafers. It is therefore possible with these coatings to selectively influence either cell adhesion or morphology.
authors.affiliation: BioChip Laboratory-CEA, 17 rue des Martyrs, 38054 Grenoble cedex 9, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Sordel
authors.forename: Frederique
authors.initials: F
authors.lastname: Kermarec-Marcel
authors.forename: Stephanie
authors.initials: S
authors.lastname: Garnier-Raveaud
authors.forename: Nicolas
authors.initials: N
authors.lastname: Glade
authors.forename: Fabien
authors.initials: F
authors.lastname: Sauter-Starace
authors.forename: Catherine
authors.initials: C
authors.lastname: Pudda
authors.forename: Mathias
authors.initials: M
authors.lastname: Borella
authors.forename: Marc
authors.initials: M
authors.lastname: Plissonnier
authors.forename: Francois
authors.initials: F
authors.lastname: Chatelain
authors.forename: Franz
authors.initials: F
authors.lastname: Bruckert
authors.forename: Nathalie
authors.initials: N
authors.lastname: Picollet-D'hahan
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000166:cytology* / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D020099:Coated Materials, Biocompatible; D006224:Cricetinae; D003412:Cricetulus; D005898:Glass; D011108:Polymers; D012825:Silicon
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; D012825:Silicon