title: A pipeline for ligand discovery using small-molecule microarrays.
abstract: Uncovering the functions of thousands of gene products, in various states of post-translational modification, is a key challenge in the post-genome era. To identify small-molecule probes for each protein function, high-throughput methods for ligand discovery are needed. In recent years, small-molecule microarrays (SMMs) have emerged as high-throughput and miniaturized screening tools for discovering protein-small-molecule interactions. Microarrays of small molecules from a variety of sources, including FDA-approved drugs, natural products and products of combinatorial chemistry and diversity-oriented synthesis, have been prepared and screened by several laboratories, leading to several newly discovered protein-ligand pairs.
authors.affiliation: Broad Institute of Harvard and MIT, 7 Cambridge Center, Cambridge, MA 02142, USA.
authors.forename: Jay L
authors.initials: JL
authors.lastname: Duffner
authors.forename: Paul A
authors.initials: PA
authors.lastname: Clemons
authors.forename: Angela N
authors.initials: AN
authors.lastname: Koehler
mesh_terms: D001688:Biological Products / Q000737:chemistry* / Q000235:genetics; D002626:Chemistry, Pharmaceutical / Q000379:methods* / Q000639:trends; D020650:Combinatorial Chemistry Techniques / Q000379:methods* / Q000639:trends; D008024:Ligands; D046228:Microarray Analysis / Q000379:methods* / Q000639:trends; D015335:Molecular Probes; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D011499:Protein Processing, Post-Translational; D012680:Sensitivity and Specificity
chemical_list: D001688:Biological Products; D008024:Ligands; D015335:Molecular Probes; D010455:Peptides