title: Nile Red nucleoside: novel nucleoside analog with a fluorophore replacing the DNA base.
abstract: A solvatochromic dye is very useful in studies of local polarity and dynamics in biological systems. A novel solvatochromic chromophore, Nile Red beta-C-2'-deoxyriboside, has been synthesized to act as a photophysical probe, and incorporated site-selectively into an oligodeoxynucleotide. The absorption and fluorescence spectra of Nile Red nucleoside showed a remarkable sensitivity to the solvent polarity.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Tainaka
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D003855:Deoxyribose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000737:chemistry*; D010078:Oxazines / Q000138:chemical synthesis / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; C520202:Nile Red beta-C-2'-deoxyriboside; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D010078:Oxazines; D012997:Solvents; D003855:Deoxyribose