title: Fibre type distribution and gene expression levels of both succinate dehydrogenase and peroxisome proliferator-activated receptor-gamma coactivator-1alpha of fibres in the soleus muscle of Zucker diabetic fatty rats.
abstract: We have reported that a change in muscle fibre type distribution is present in two strains of diabetic rats (Otsuka Long-Evans Tokushima Fatty and Goto-Kakizaki rats). In this study, we determined whether the change in soleus muscle fibre type distribution was caused by diabetes, using obese, diabetic (Zucker diabetic fatty, ZDF), obese, non-diabetic (Zucker fatty, ZF) and non-diabetic, non-obese rats (Zucker lean, ZL). Moreover, we investigated whether the gene expression levels of metabolic key molecules, namely the transcriptional factors of metabolic genes, exemplified by peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha), and the oxidative enzymes in mitochondria, exemplified by succinate dehydrogenase (SDH), were changed in type I and II muscle fibres in each type of rat, using the new technique of laser capture microdissection (LCM). Both plasma glucose and glucosylated haemoglobin levels were significantly higher in ZDF than in ZL and ZF rats. A lower percentage of type IIA fibres was observed in the muscles of ZDF rats than in those of ZL and ZF rats. The mRNA expression levels of SDH in type II fibres and of PGC-1alpha in type I fibres were significantly lower in ZDF than in ZL and ZF rats as assessed by LCM and real-time PCR analysis. We have shown, for the first time, that a lower percentage of type IIA fibres was observed in ZDF rats. We have also discovered that the expression levels of the oxidative metabolism-related genes, PGC-1alpha and SDH, decreased in type I and type II fibres, respectively, of ZDF rats.
authors.affiliation: Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan. adachi-tet@umin.ac.jf
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Adachi
authors.forename: Naoko
authors.initials: N
authors.lastname: Kikuchi
authors.forename: Koichiro
authors.initials: K
authors.lastname: Yasuda
authors.forename: Reiko
authors.initials: R
authors.lastname: Anahara
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Yamamura
authors.forename: Chisato
authors.initials: C
authors.lastname: Mori
authors.forename: Gozoh
authors.initials: G
authors.lastname: Tsujimoto
authors.forename: Kinsuke
authors.initials: K
authors.lastname: Tsuda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ishihara
mesh_terms: D000273:Adipose Tissue / Q000473:pathology; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D003920:Diabetes Mellitus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D015870:Gene Expression; D006442:Glycated Hemoglobin / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007834:Lasers; D008297:Male; D042282:Microdissection / Q000379:methods; D018656:Muscle Fibers, Fast-Twitch / Q000737:chemistry / Q000201:enzymology; D018485:Muscle Fibers, Skeletal / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology; D018657:Muscle Fibers, Slow-Twitch / Q000737:chemistry* / Q000201:enzymology; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology; D009765:Obesity / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009929:Organ Size; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D051381:Rats; D011924:Rats, Zucker; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013385:Succinate Dehydrogenase / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis* / Q000235:genetics
chemical_list: D001786:Blood Glucose; D006442:Glycated Hemoglobin; D007328:Insulin; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D013385:Succinate Dehydrogenase; D014157:Transcription Factors; C501429:Ppargc1a protein, rat