title: Manipulation of double-stranded DNA by artificial restriction enzyme composed of Ce(IV)/EDTA and PNA.
abstract: Through the invasion of pseudo-complementary PNA (pePNA) to double-stranded DNA, gap-like structures were formed at predetermined sites in both strands of PBR322 plasmid DNA. These gap-like sites were selectively hydrolyzed by Ce(IV)/EDTA complex, and two designed fragments were obtained. Furthermore, the scission fragment by this artificial restriction enzyme was successfully ligated with foreign DNA.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Yoji
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Masao
authors.initials: M
authors.lastname: Mori
authors.forename: Takafumi
authors.initials: T
authors.lastname: Tomita
authors.forename: Jing-Min
authors.initials: JM
authors.lastname: Zhou
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D002563:Cerium / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004492:Edetic Acid / Q000378:metabolism*; D020135:Peptide Nucleic Acids / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism
chemical_list: D020135:Peptide Nucleic Acids; D002563:Cerium; D004247:DNA; D004492:Edetic Acid; D004262:DNA Restriction Enzymes