Antisense activity of 2',4'-BNA targeted to bcl-xL gene in HepG2 cell.
Introduction of the 2',4'-BNA monomer into oligonucleotides significantly enhanced binding affinity toward ssRNA and resistance to nuclease degradation. Here, we evaluated the antisense activity of 2',4'-BNA oligonucleotides against bcl-xL in HepG2 cells. Our data revealed that 2',4'-BNA antisense oligonucleotides remarkably inhibited the expression of bcl-xL in HepG2 cells compared to the natural DNA antisense oligonucleotide. The inhibitory effect of 2',4'-BNA antisense oligonucleotide was dose-dependent and highly sequence-specific.
Roongjang
Somjing
S
Graduate School of Pharmaceutical Science, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Takahashi
Kurena
K
Park
Myunji
M
Obika
Satoshi
S
Imanishi
Takeshi
T
D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051020:bcl-X Protein / Q000096:biosynthesis* / Q000235:genetics
D001952:Bridged-Ring Compounds; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D051020:bcl-X Protein