Evidence that RpoS (sigmaS) in Borrelia burgdorferi is controlled directly by RpoN (sigma54/sigmaN).
The alternative sigma factor (RpoN-RpoS) pathway controls the expression of key virulence factors in Borrelia burgdorferi. However, evidence to support whether RpoN controls rpoS directly or, perhaps, indirectly via a transactivator has been lacking. Herein we provide biochemical and genetic evidence that RpoN directly controls rpoS in B. burgdorferi.
Smith
Alexandra H
AH
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9048, USA.
Blevins
Jon S
JS
Bachlani
Gulnaz N
GN
Yang
Xiaofeng F
XF
Norgard
Michael V
MV
D000942:Antigens, Bacterial / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D025065:Borrelia burgdorferi / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D051877:RNA Polymerase Sigma 54 / Q000502:physiology*; D012808:Sigma Factor / Q000235:genetics*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C079616:OspC protein; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D051877:RNA Polymerase Sigma 54