title: Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene.
abstract: We studied the transcriptional regulation of the human mitochondrial translation initiation factor 2 (IF2mt) gene. The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes. Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2alpha/beta binds to the IF2mt promoter. Reporter assays, where HEK293T cells were co-transfected with an NRF-2alpha/beta-expressing vector and/or an IF2mt promoter reporter vector, revealed that NRF-2 trans-activates the IF2mt promoter. NRF-2 sites were also found in the promoters of several other mitochondrial translation factors, which suggests NRF-2 may play a key role in the regulation of mitochondrial protein synthesis.
authors.affiliation: Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Building FSB-401, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture 277-8562, Japan.
authors.forename: Rippei
authors.initials: R
authors.lastname: Hayashi
authors.forename: Takuya
authors.initials: T
authors.lastname: Ueda
authors.forename: Mary A
authors.initials: MA
authors.lastname: Farwell
authors.forename: Nono
authors.initials: N
authors.lastname: Takeuchi
mesh_terms: D002460:Cell Line; D017931:DNA Primers; D051268:GA-Binding Protein Transcription Factor / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D007668:Kidney; D008969:Molecular Sequence Data; D010957:Plasmids; D039881:Prokaryotic Initiation Factor-2 / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D017931:DNA Primers; D051268:GA-Binding Protein Transcription Factor; D039881:Prokaryotic Initiation Factor-2