title: Recognition of DNA with assembly of minor groove binders mediated by metal complexation.
abstract: Genome-targeting molecules would have broad application as probes in genomic study. We recently developed the ligands (Bpy-H) connecting the Hoechst33258 skeleton for DNA binding and 2,2'-bipyridine for Cu(2+) complexation. It was shown that L-Bpy-H with a long linker exhibited Cu(2+)-mediated assembly on the DNA template having two A(3)T(3) sites in a ratio of 1:2:1 DNA-ligand-Cu(2+). Binding selectivity depends on the distance between the two A(3)T(3) sites of DNA. In contrast, S-Bpy-H having a short linker tended to form 1:2:2 DNA-ligand-Cu(2+) complex.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Kataoka
authors.forename: Mikimasa
authors.initials: M
authors.lastname: Tanada
authors.forename: Ippei
authors.initials: I
authors.lastname: Onishi
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sasaki
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D001562:Benzimidazoles / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D008024:Ligands; D009690:Nucleic Acid Conformation
chemical_list: D001562:Benzimidazoles; C522088:L-Bpy-H compound; D008024:Ligands; C522089:S-Bpy-H compound; D015082:2,2'-Dipyridyl; D003300:Copper; D004247:DNA