HIV gene therapy using RNA virus systems.
We designed a vector to produce single-stranded DNA (ssDNA). We used HIV-1 reverse transcription for the purpose of constructing a DNAzyme expression vector against the HIV-1 env V3 loop. Initiation of HIV-1 reverse transcription requires the formation of a complex containing the viral RNA, tRNALys and reverse transcriptase. The expression vector contains the HIV-1 primer binding site (PBS) and tRNALys at the 3' end of its RNA transcript, thus enabling to an ssDNA would be synthesized by HIV-1 reverse transcriptase. We have demonstrated that the DNAzyme expressed by the lentiviral vectors suppressed HIV-1 replication in SupT1 cells.
Hayafune
Masaaki
M
Department of Life and Environmental Science, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.
Miyano-Kurosaki
Naoko
N
Kusunoki
Akiko
A
Mouri
Yuka
Y
Takaku
Hiroshi
H
D002460:Cell Line; D021881:DNA, Catalytic / Q000096:biosynthesis / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000096:biosynthesis; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D015658:HIV Infections / Q000628:therapy; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D012367:RNA, Viral / Q000378:metabolism; D048348:Reverse Transcription; D014779:Virus Replication
D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D012367:RNA, Viral