Circular dichroism spectra of d(CGCGCGCGCGCG): evidence for intermediate models in the B-to-Z transition.
We analyze CD spectra of d(CGCGCGCGCGCG) to study the potential intermediate states produced during transition between both types of secondary structures of B- and Z-forms as ionic strength of PBS buffer increases. Decomposition of the CD spectra by linear superposition provides evidence for three intermediate states that distinct from B and Z-DNA. Intermediate states of B-form and Z-form found from CD spectra analysis are discussed in terms of early proposed by others the models for B- to Z-DNA transition and W-DNA model refined by molecular modelling program.
Grzeskowiak
Kazimierz
K
Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Ohishi
Hirofumi
H
Ivanov
Vasilli
V
D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D043542:DNA, Z-Form / Q000737:chemistry*; D018900:Dinucleotide Repeats; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry; D009690:Nucleic Acid Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry
D043542:DNA, Z-Form; D012710:Serum Albumin, Bovine; D004247:DNA; D009113:Muramidase