Electrochemical detection of single base mismatches in DNA using redox-modified oligonucleotides.
A facile synthesis of new anthraquinone (AQ)-modified oligonucleotides was described. The AQ-oligomer-bound electrode was used for the electrochemical detection of a single base mismatch in DNA.
Orino
Narihisa
N
Department of Materials Science and Chemistry, University of Hyogo, 2167 Shosha, Himeji 671-2201, Japan.
Kumamoto
Satoshi
S
Nakamura
Mitsunobu
M
Yamana
Kazushige
K
D000880:Anthraquinones / Q000138:chemical synthesis / Q000737:chemistry*; D020137:Base Pair Mismatch; D004252:DNA Mutational Analysis / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D015345:Oligonucleotide Probes / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010084:Oxidation-Reduction
C520200:3-(9,10-anthraquinone-2-carboxamido)-2-cyanoethyl-4-(dimethoxytrityl)butoxy-N,N-diisopropylphosphoramidate; D000880:Anthraquinones; D015345:Oligonucleotide Probes; D009943:Organophosphorus Compounds