title: Mixed gastric carcinomas show similar chromosomal aberrations in both their diffuse and glandular components.
abstract: Gastric cancer is one of the most frequent malignancies in the world. Nonetheless, the knowledge of the molecular events involved in the development of gastric carcinoma is far from complete. One of the hallmarks of gastric cancer is chromosomal instability resulting in abnormal DNA copy number changes throughout the genome. Mixed gastric carcinomas constitute a rare histological entity, containing the two main histological phenotypes (diffuse and intestinal). Very little is known about the underlying mechanisms of phenotypic divergence in these mixed tumours. To the best of our knowledge only E-Cadherin mutations were implicated so far in the divergence of these tumours and nothing is known about the involvement of chromosome copy number changes in the two divergent histological components. In this study, we compared the DNA copy number changes, in the two different components (diffuse and intestinal) of mixed gastric carcinomas by microarray - comparative genomic hybridisation (array CGH). The analysis of 12 mixed gastric carcinomas showed no significant differences in array CGH profiles between the diffuse and intestinal components of mixed carcinomas. This supports the idea that the phenotypic divergence within mixed gastric carcinomas is not caused by DNA chromosomal aberrations.
authors.affiliation: Department of Pathology, VUMC - VU University Medical Centre, Amsterdam, The Netherlands. b.carvalho@vumc.nl
authors.forename: Beatriz
authors.initials: B
authors.lastname: Carvalho
authors.forename: Tineke E
authors.initials: TE
authors.lastname: Buffart
authors.forename: Rui M
authors.initials: RM
authors.lastname: Reis
authors.forename: Thomas
authors.initials: T
authors.lastname: Mons
authors.forename: Cátia
authors.initials: C
authors.lastname: Moutinho
authors.forename: Paula
authors.initials: P
authors.lastname: Silva
authors.forename: Nicole C T
authors.initials: NC
authors.lastname: van Grieken
authors.forename: Heike
authors.initials: H
authors.lastname: Grabsch
authors.forename: Cornelis J H
authors.initials: CJ
authors.lastname: van de Velde
authors.forename: Bauke
authors.initials: B
authors.lastname: Ylstra
authors.forename: Gerrit A
authors.initials: GA
authors.lastname: Meijer
authors.forename: Fátima
authors.initials: F
authors.lastname: Carneiro
mesh_terms: D002869:Chromosome Aberrations; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D018628:Gene Dosage; D015894:Genome, Human; D006801:Humans; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm