Quantitative immunohistochemical detection of the molecular expression patterns in proliferative inflammatory atrophy.
The proliferative inflammatory atrophy (PIA) is considered as a possible precursor of prostate intraepithelial neoplasia (PIN) or prostate cancer (PCa). In this study we assessed quantitatively the expression of AMACR, p63, COX-2, GST and iNOS in serial paraffin-embedded tissue sections obtained after radical prostatectomy of PCa patients (n = 30). The applicability of these markers to distinguish PIA, PIN and PCa was evaluated. We also compared the immunohistochemical expression profiles of AMACR, COX-2 and GST in the luminal and basal cells in lesions of PIN arisen in PIA or PIA alone. Two different patterns of COX-2 expression according to the p63 status of the basal cells were found. This observation gives us grounds to hypothesize that the diverse COX-2 patterns resulted from an initial basal cell damage which subsequently propagated to its luminal secretory cells progeny.
Karaivanov
M
M
Department of General and Clinical Pathology, Medical University of Pleven, Pleven, Bulgaria.
Todorova
K
K
Kuzmanov
A
A
Hayrabedyan
S
S
D000368:Aged; D000369:Aged, 80 and over; D001284:Atrophy / Q000473:pathology; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D049109:Cell Proliferation; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000201:enzymology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D011230:Precancerous Conditions / Q000201:enzymology* / Q000473:pathology; D011467:Prostate / Q000201:enzymology / Q000473:pathology; D019048:Prostatic Intraepithelial Neoplasia / Q000201:enzymology* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000473:pathology
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins