Base-discriminating fluorescent DNA probe based on the guanine-specific fluorescence quenching.
A new strategy for the detection of a single base alteration utilizing the fluorescence quenching by guanine base is described. We have devised a novel base-discrimination fluorescent (BDF) nucleoside, PyT, which contains pyrene carboxamide fluorophore at sugar 4' position. PyT-containing oligodeoxynucleotides (ODNs) exhibit an intense fluorescence only when PyT forms a complementary base pair with adenine.
Dohno
Chikara
C
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8510, Japan.
Saito
Isao
I
D020029:Base Pairing; D015342:DNA Probes / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D015342:DNA Probes; D005456:Fluorescent Dyes; D006147:Guanine