Archaeal minichromosome maintenance (MCM) helicase can unwind DNA bound by archaeal histones and transcription factors.
Protein-DNA complexes must be disassembled to facilitate DNA replication. Replication forks contain a helicase that unwinds the duplex DNA at the front of the fork. The minichromosome maintenance helicase from the archaeon Methanothermobacter thermautotrophicus required only ATP to unwind DNA bound into complexes by the M. thermautotrophicus archaeal histone HMtA2, transcription repressor TrpY, or into a transcription pre-initiation complex by M. thermautotrophicus TATA-box-binding protein, transcription factor B, and RNA polymerase. In contrast, the minichromosome maintenance helicase was unable to unwind DNA bound by this archaeal RNA polymerase in a stalled transcript-elongating complex.
Shin
Jae-Ho
JH
Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850 and the.
Santangelo
Thomas J
TJ
Department of Microbiology, Ohio State University, Columbus, Ohio 43210.
Xie
Yunwei
Y
Department of Microbiology, Ohio State University, Columbus, Ohio 43210.
Reeve
John N
JN
Department of Microbiology, Ohio State University, Columbus, Ohio 43210.
Kelman
Zvi
Z
Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850 and the. Electronic address: kelman@umbi.umd.edu.
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D019843:Archaeal Proteins / Q000737:chemistry / Q000378:metabolism*; D004265:DNA Helicases / Q000737:chemistry / Q000378:metabolism*; D004261:DNA Replication / Q000502:physiology*; D019641:DNA, Archaeal / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D017013:Methanobacteriaceae / Q000201:enzymology*; D035181:TATA-Box Binding Protein / Q000737:chemistry / Q000378:metabolism; D035581:Transcription Factor TFIIB / Q000737:chemistry / Q000378:metabolism*
D019843:Archaeal Proteins; D019641:DNA, Archaeal; D004268:DNA-Binding Proteins; D006657:Histones; D035181:TATA-Box Binding Protein; D035581:Transcription Factor TFIIB; C106466:transcription factor B, Archaea; C078607:HmtB protein, Methanothermobacter thermautotrophicus; D000255:Adenosine Triphosphate; D012321:DNA-Directed RNA Polymerases; D004265:DNA Helicases