Analysis of protein expression profiles of Halobacillus dabanensis D-8T under optimal and high salinity conditions.
Two-dimensional (2-D) gel electrophoresis was employed to display the expression profiles of proteins of Halobacillus dabanensis D-8(T) under 1%, 10%, and 20% salinities. Approximately 700 protein spots could be detected in the 2-D gels by Imagemastertrade mark 2D Platinum software. The molecular masses of the majority of intracellular proteins were distributed in the range of 17.5 kDa-66 kDa and isoelectric points of 4.0-5.9. In total 133 protein spots were observed with a changed expression level under different salinity conditions. Sixty-two protein spots showed upregulation and 26 new protein spots were found under high salinity conditions, while 25 protein spots were downregulated and 20 spots disappeared. Twenty-seven proteins with a markedly changed expression in hypersaline environments were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF/MS) and MASCOT. A changed expression pattern was observed for proteins related to energy-producing pathways, stress regulators, and proteins involved in the survival of strain D-8(T) under high salt challenges. Many proteins play necessary roles in the adaptation to high salt or as a general stress protein, and some proteins are salt-stressed specific proteins that improve the capability of salt-tolerance of strain D-8(T) growth under extremely hypersaline condition.
Feng
De Qin
DQ
Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University and Key Laboratory of Agro-Microbial Resources and Application of Agricultural Ministry, Beijing 100094, China.
Yang
Li Fu
LF
Lu
Wei Dong
WD
Yang
Su Sheng
SS
D001406:Bacillaceae / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D007526:Isoelectric Point; D008970:Molecular Weight; D040901:Proteomics / Q000379:methods*; D012965:Sodium Chloride / Q000032:analysis*; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
D001426:Bacterial Proteins; D012965:Sodium Chloride