title: CpG oligodeoxynucleotides allow for effective adoptive T-cell therapy in chronic retroviral infection.
abstract: Adoptive T-cell therapy in cancer or chronic viral infections is often impeded by the development of functional impairment of the transferred cells. To overcome this therapeutic limitation we combined adoptive transfer of naive, virus-specific CD8+ T cells with immunostimulative CpG oligodeoxynucleotides (ODNs) in mice chronically infected with the Friend retrovirus. The CpG-ODN co-injection prevented the T cells from developing functional defects in IFNgamma and granzyme production and degranulation of cytotoxic molecules. Thus, the transferred T cells were able to reduce chronic viral loads when combined with CpG-ODNs. This strategy provides a new approach for developing successful adoptive T-cell therapy against chronic infections.
authors.affiliation: Institute for Virology, University of Duisburg-Essen, Essen, Germany.
authors.forename: Anke R M
authors.initials: AR
authors.lastname: Kraft
authors.forename: Frank
authors.initials: F
authors.lastname: Krux
authors.forename: Simone
authors.initials: S
authors.lastname: Schimmer
authors.forename: Claes
authors.initials: C
authors.lastname: Ohlen
authors.forename: Philip D
authors.initials: PD
authors.lastname: Greenberg
authors.forename: Ulf
authors.initials: U
authors.lastname: Dittmer
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000201:enzymology / Q000276:immunology* / Q000502:physiology; D002908:Chronic Disease; D005089:Exocytosis; D005622:Friend murine leukemia virus; D053804:Granzymes / Q000096:biosynthesis; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007371:Interferon-gamma / Q000096:biosynthesis; D007942:Leukemia, Experimental / Q000276:immunology / Q000628:therapy; D051379:Mice; D008822:Mice, Transgenic; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D012192:Retroviridae Infections / Q000276:immunology* / Q000628:therapy*; D014412:Tumor Virus Infections / Q000276:immunology / Q000628:therapy
chemical_list: C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D007371:Interferon-gamma; D053804:Granzymes