UVA-induced degradation of 8-hydroxyguanine in oligonucleotide and the effect on its activities in yeast oligonucleotide transformation assay.
UVA-induced conversion of 8-hydroxyguanine in oligonucleotides was studied. By irradiation with 334 nm UVA light, 8-hydroxyguanine was completely changed to unknown compounds. Monomeric nucleoside may be much less labile to UVA. Mutagenic specificities of 8-hydroxyguanine were investigated using yeast oligonucleotide mutation assay. UVA irradiation moderately reduced the activity of the oligonucleotides.
Okugawa
Yoji
Y
Department of Genomics and Proteomics, Okayama University Advanced Science Research Center, Tsushima, Okayama 700-8530, Japan.
Otsuka
Chie
C
Loakes
David
D
Sugiyama
Hiroshi
H
Negishi
Kazuo
K
D001681:Biological Assay / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D016296:Mutagenesis; D009841:Oligonucleotides / Q000378:metabolism* / Q000528:radiation effects*; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000528:radiation effects*; D014466:Ultraviolet Rays
D009841:Oligonucleotides; C024829:8-hydroxyguanine; D006147:Guanine