title: [Transport mechanism of danshensu across Caco-2 monolayer model].
abstract: OBJECTIVE
  To study the absorption mechanism of Danshensu by using Caco-2 monolayer model .
  
  
  METHODS
  Caco-2 cell monolayer model was used to study the bi-direction transport of Danshensu. An LC-MS method was developed to measure the concentration of Danshensu in cell culture medium and calculate the apparent permeability coefficients (P(app)). The effects of time, drug concentration and inhibitor on the absorption of Danshensu were studied.
  
  
  RESULTS
  Transport of Danshensu was time and concentration dependent, and it was also effected by P-glycoprotein inhibitor. P(app) increased with time increase and tended to become saturated at some point. It, however,decreased while concentration of Danshensu increased. P(ratio) is larger than 1.5 . Verapamil can cause significantly effect on transport of Danshensu: P(app,A-B) increased and P(app,B-A) decreased .
  
  
  CONCLUSIONS
  The absorption of Danshensu in Caco-2 cell model may be an active transportation mediated by P-glycoprotein transporter.
authors.affiliation: Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310027, China.
authors.forename: Di-Feng
authors.initials: DF
authors.lastname: Zhu
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Zhao
authors.forename: Yi-Yu
authors.initials: YY
authors.lastname: Cheng
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006801:Humans; D007773:Lactates / Q000008:administration & dosage / Q000493:pharmacokinetics*; D010946:Plants, Medicinal / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry; D014700:Verapamil / Q000494:pharmacology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004365:Drugs, Chinese Herbal; D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid; D014700:Verapamil