Mechanism of PNA transport to the nuclear compartment.
We evaluated the nuclear uptake of fluorescently labeled peptide nucleic acids and measured the binding of unlabeled peptide nucleic acids (PNAs) to the endogenous HER-2/neu promotor in digitonin-permeabilized SK-BR-3 cells. Fluorescently labeled PNAs readily enter the nucleus of digitonin-permeabilized cells, and binding to the chromosomal target sequence was detected with a bis-PNA. Nuclear uptake and target sequence binding were inhibited by N-ethylmaleimide (NEM) and GTPgammaS. We conclude that PNAs are transported into the nucleus through an energy-dependent process involving the nuclear pore complex.
Stankova
Lenka
L
Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724-5024, USA.
Ziemba
Amy J
AJ
Zhilina
Zhanna V
ZV
Ebbinghaus
Scot W
SW
D021581:Active Transport, Cell Nucleus; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002875:Chromosomes / Q000378:metabolism; D005456:Fluorescent Dyes; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006801:Humans; D022022:Nuclear Pore; D020135:Peptide Nucleic Acids / Q000493:pharmacokinetics*; D011401:Promoter Regions, Genetic; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics
D005456:Fluorescent Dyes; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D020135:Peptide Nucleic Acids; D018719:Erb-b2 Receptor Tyrosine Kinases