Simultaneous determination of 12 major constituents in Forsythia suspensa by high performance liquid chromatography--DAD method.
A high performance liquid chromatographic method was developed to simultaneously determine the 12 major constituents of Forsythia suspensa, namely R-suspensaside, S-suspensaside, S-suspensaside methyl ether, (+)-pinoresinol-beta-d-glucoside, forsythiaside, (+)-epipinoresinol-4'-O-glucoside, suspensaside A, rutin, phillyrin, (+)-pinoresinol, (+)-epipinoresinol and phillygenin. The HPLC assay was performed on a Zorbax XDB C(18) column with gradient elution of methanol and 0.3% aqueous acetic acid within 55 min. The detection wavelength was 280 nm. All the compounds showed good linearity (r(2)>0.9998). The method was reproducible with intra- and inter-day variation less than 3%. The recovery of the assay was in the range of 91.2-104.9%. The method was successfully applied to the quantification of 12 constituents in 33 F. suspensa samples. The results indicated that the developed assay could be considered as a suitable quality control method for F. suspensa.
Guo
Hui
H
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100083, PR China.
Liu
Ai-Hua
AH
Li
Lie
L
Guo
De-An
DA
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D031657:Forsythia / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D012996:Solutions; D012997:Solvents
D010936:Plant Extracts; D012996:Solutions; D012997:Solvents; D000431:Ethanol