title: Crystallization of ornithine acetyltransferase from yeast by counter-diffusion and preliminary X-ray study.
abstract: A study is presented on the crystallization of ornithine acetyltransferase from yeast, which catalyzes the fifth step in microbial arginine synthesis. The use of the counter-diffusion technique removes the disorder present in one dimension in crystals grown by either the batch or hanging-drop techniques. This makes the difference between useless crystals and crystals that allow successful determination of the structure of the protein. The crystals belong to space group P4, with unit-cell parameters a = b = 66.98, c = 427.09 A, and a data set was collected to 2.76 A.
authors.affiliation: Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel and Vlaams Interuniversitair Instituut voor Biotechnologie, Pleinlaan 2, B-1050 Brussels, Belgium. dominique.maes@vub.ac.be
authors.forename: Dominique
authors.initials: D
authors.lastname: Maes
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Crabeel
authors.forename: Cécile
authors.initials: C
authors.lastname: Van de Weerdt
authors.forename: Joseph
authors.initials: J
authors.lastname: Martial
authors.forename: Eveline
authors.initials: E
authors.lastname: Peeters
authors.forename: Daniël
authors.initials: D
authors.lastname: Charlier
authors.forename: Klaas
authors.initials: K
authors.lastname: Decanniere
authors.forename: Celine
authors.initials: C
authors.lastname: Vanhee
authors.forename: Lode
authors.initials: L
authors.lastname: Wyns
authors.forename: Ingrid
authors.initials: I
authors.lastname: Zegers
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004058:Diffusion; D005782:Gels; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012685:Sepharose
chemical_list: D005782:Gels; D012685:Sepharose; D000123:Acetyltransferases; C020646:glutamate acetyltransferase