Crosslinked hydrogels for tympanic membrane repair.
OBJECTIVE
To provide a less expensive and more convenient protocol for the treatment of tympanic membrane perforations (TMPs).


METHODS
Several materials were prepared and compared for TMP repair including Carbylan-SX, Gelatin-DTPH-PEGDA (GX), Carbylan-S/Gelatin-DTPH (Carbylan-GSX) (injectable and sponge), Gelfoam, Epifilm, and crosslinked thiolated chondroitin sulfate (CS-DTPH-PEGDA [CS-SX]). Hartley pigmented guinea pigs (Elm Hill) underwent bilateral myringotomy with 1 ear left as a control and the other treated with one of the previously mentioned materials.


RESULTS
Carbylan-GSX (injectable and sponge), Gelfoam with saline, and CS-SX had the shortest time for TMP closure. Epifilm, Carbylan, and gelatin preparations resulted in closure rates similar to controls. CS-SX showed a marked inflammatory reaction compared with controls and other materials based on neutrophil, lymphocyte, epitheloid counts, and degree of fibrosis.


CONCLUSIONS
This study shows the validity of Carbylan-GSX compared with Gelfoam as a material to promote TMP closure in an acute TMP guinea pig model.
Park
Albert H
AH
Division of Otolaryngology-Head and Neck Surgery, University of Utah, Salt Lake City, Utah 84132, USA. pcapark@ihc.com
Hughes
Casey W
CW
Jackson
Adrienne
A
Hunter
Lisa
L
McGill
Lawrence
L
Simonsen
Sara Ellis
SE
Alder
Stephen C
SC
Shu
Xiao Zheng
XZ
Prestwich
Glenn D
GD
D000818:Animals; D002809:Chondroitin Sulfates / Q000008:administration & dosage; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000737:chemistry; D005780:Gelatin / Q000008:administration & dosage; D005781:Gelatin Sponge, Absorbable / Q000008:administration & dosage; D006168:Guinea Pigs; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives*; D020100:Hydrogels / Q000008:administration & dosage; D011092:Polyethylene Glycols / Q000008:administration & dosage; D018058:Tympanic Membrane Perforation / Q000628:therapy*
C509315:HA-DTPH-PEGDA; D020100:Hydrogels; C513409:carbylan-GSX; D011092:Polyethylene Glycols; D005780:Gelatin; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates