title: Regiospecificity profiles of storage and membrane lipids from the gill and muscle tissue of Atlantic salmon (Salmo salar L.) grown at elevated temperature.
abstract: Regiospecific and traditional analysis, of both storage and membrane lipids, was performed on gill, white muscle, and red muscle samples taken from Atlantic salmon (Salmo salar) to gauge the effect of elevated water temperature. The fish, fed a commercial diet, were held at an elevated water temperature of 19 degrees C. Total n-3 PUFA, total PUFA, and n-3/n-6 and unsaturated/saturated fatty acid (UFA/SFA) ratios in the FA profile of the total lipid extract in the white muscle were fairly low compared with fish grown at 15 degrees C. Adaptation of structural and storage lipids at elevated temperatures was shown by a significant (P < 0.01) reduction in PUFA especially in the percentage of EPA (6-8%). Further adaptation was indicated by the percentages of SFA, which were significantly (P< 0.05) higher in gill (56%) and white muscle (58%) polar lipid fractions and coincided with lower percentages of n-3, n-6, and total PUFA. The regiospecific profiles indicated a high affinity of DHA to the sn-2 position in both the TAG (61-68%) and polar lipid (35-60%) fractions. The combination of detailed regiospecific and lipid analyses demonstrated adaptation of cell membrane structure in Atlantic salmon grown at an elevated water temperature.
authors.affiliation: School of Aquaculture, Tasmanian Aquaculture and Fisheries Institute, University of Tasmania (UTAS), Launceston, Tasmania 7250, Australia. matthew.miller@csiro.au
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Miller
authors.forename: Peter D
authors.initials: PD
authors.lastname: Nichols
authors.forename: Julia
authors.initials: J
authors.lastname: Barnes
authors.forename: Noel W
authors.initials: NW
authors.lastname: Davies
authors.forename: Evan J
authors.initials: EJ
authors.lastname: Peacock
authors.forename: Chris G
authors.initials: CG
authors.lastname: Carter
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D005227:Fatty Acids / Q000032:analysis*; D005526:Food, Formulated; D005880:Gills / Q000737:chemistry* / Q000378:metabolism; D008563:Membrane Lipids / Q000032:analysis* / Q000145:classification / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D020317:Salmo salar / Q000254:growth & development / Q000378:metabolism; D013696:Temperature
chemical_list: D005227:Fatty Acids; D008563:Membrane Lipids