Design, synthesis and structural analysis of novel bent DNAs containing cyclic uridylate dimer analogues.
We developed novel bent DNAs containing cyclic uridylate dimmer analogues (Figure 1, 1a-c), which possess an alkylene linkage with various length. These structural properties were revealed by fluorescence energy transfer (FRET) experiments, and their bending angles were found to be quite deep. This result suggests that our strategy can be applicable to highly refined studies to analyze various structure-specific DNA recognitions.
Murata
Shunpei
S
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Mizumura
Yoko
Y
Ichikawa
Satoshi
S
Matsuda
Akira
A
D001952:Bridged-Ring Compounds / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry
D001952:Bridged-Ring Compounds; D009838:Oligodeoxyribonucleotides; D004247:DNA; D014529:Uridine