Tolerance of transgenic canola plants (Brassica napus) amended with plant growth-promoting bacteria to flooding stress at a metal-contaminated field site.
The growth of transgenic canola (Brassica napus) expressing a gene for the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase was compared to non-transformed canola exposed to flooding and elevated soil Ni concentration, in situ. In addition, the ability of the plant growth-promoting bacterium Pseudomonas putida UW4, which also expresses ACC deaminase, to facilitate the growth of non-transformed and transgenic canola under the above mentioned conditions was examined. Transgenic canola and/or canola treated with P. putida UW4 had greater shoot biomass compared to non-transformed canola under low flood-stress conditions. Under high flood-stress conditions, shoot biomass was reduced and Ni accumulation was increased in all instances relative to low flood-stress conditions. This is the first field study to document the increase in plant tolerance utilizing transgenic plants and plant growth-promoting bacteria exposed to multiple stressors.
Farwell
Andrea J
AJ
Department of Biology, University of Waterloo, Waterloo, Ontario, Canada. afarwell@sciborg.uwaterloo.ca
Vesely
Susanne
S
Nero
Vincent
V
Rodriguez
Hilda
H
McCormack
Kimberley
K
Shah
Saleh
S
Dixon
D George
DG
Glick
Bernard R
BR
D018533:Biomass; D029688:Brassica napus / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology; D019755:Carbon-Carbon Lyases / Q000235:genetics; D004190:Disasters; D004781:Environmental Exposure / Q000009:adverse effects; D015870:Gene Expression; D018525:Germination / Q000235:genetics / Q000502:physiology; D009532:Nickel / Q000633:toxicity*; D018517:Plant Roots / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000254:growth & development* / Q000382:microbiology; D016958:Pseudomonas putida / Q000235:genetics / Q000502:physiology*; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D012987:Soil / Q000032:analysis; D012989:Soil Pollutants / Q000633:toxicity*
D012987:Soil; D012989:Soil Pollutants; D009532:Nickel; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases