Microwave assisted rolling circle amplification.
The Rolling Circle Amplification is an effective method of DNA amplification having tandem repeated sequences. Firstly, the template sequence is designed to form a circular structure by the annealing with the primer. The thermostable polymerase is catalyzed to primer and circular template DNA. As a result, tandem repetitive olignucleotide of circular template sequence can be obtained. In our recent study, Bst DNA polymerase catalyzed RCA reaction had been elucidated that the oligonucleotides with tandem repeated sequence were obtained as the ladder band and amplified the enough amounts of DNA within short time. On the other hand, we also focused on the microwave technology for advance to the various chemical and biological reactions. In this study, the microwave was irradiated to RCA reaction on controlling the temperature.
Yoshimura
Takeo
T
Department of Biological Functions and Engineering, Kyushu Institute of Technology, Wakamatsu, Kitakyushu, Fukuoka 808-0196, Japan.
Nishida
Kunitada
K
Uchibayashi
Keiichi
K
Ohuchi
Shokichi
S
D004247:DNA / Q000096:biosynthesis*; D004270:DNA, Circular / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism; D004795:Enzyme Stability; D008872:Microwaves; D021141:Nucleic Acid Amplification Techniques; D009841:Oligonucleotides / Q000737:chemistry / Q000528:radiation effects; D020080:Tandem Repeat Sequences; D013696:Temperature; D013698:Templates, Genetic
D004270:DNA, Circular; D009841:Oligonucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase