title: Activity of nucleotide excision repair enzymes for oxanine cross-link lesions.
abstract: Nitric oxide and nitrous acid induce deamination of DNA bases, resulting in uracil, hypoxanthine, xanthine, and oxanine (Oxa) as major damage. Oxa reacts further with polyamines and DNA binding proteins, generating bulky cross-link adducts. Recently we have shown Oxa and cross-link adducts are potentially genotoxic lesions. In the present study, we have assessed the role of base excision repair (BER) and nucleotide excision repair (NER) systems in the repair of Oxa and Oxa-spermine (Oxa-Sp) cross-link adducts. Oxa was very poorly removed from DNA by both BER glycosylases and NER enzymes, whereas Oxa-Sp was efficiently excised by E. coli and human NER enzymes.
authors.affiliation: Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakano
authors.forename: Atsushi
authors.initials: A
authors.lastname: Katafuchi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Terato
authors.forename: Toshinori
authors.initials: T
authors.lastname: Suzuki
authors.forename: Bennett
authors.initials: B
authors.lastname: Van Houten
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ide
mesh_terms: D018736:DNA Adducts / Q000737:chemistry / Q000378:metabolism*; D045647:DNA Glycosylases / Q000378:metabolism; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000378:metabolism*; D006801:Humans; D011684:Purine Nucleosides / Q000737:chemistry / Q000378:metabolism*; D013096:Spermine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D018736:DNA Adducts; D011684:Purine Nucleosides; C468766:oxanine; D013096:Spermine; D045647:DNA Glycosylases; D045643:DNA Repair Enzymes