Fabrication of Au-DNA-Au nanostructure with new-type DNA-Au conjugate.
Nano devices fabricated from DNA and nanoparticles found many important applications in both biotechnology and nanotechnology. Conventionally, DNA was linked to gold surfaces through a linker. In this study, DNAs were directly attached to gold surfaces through S-Au bonds by modifying 5'-end of DNA with a mercapto group. The pH of the reaction mixture played an important role in DNA-gold conjugate fabrication. The DNA-Au conjugates were successfully obtained in pure forms and, with the use of this new type conjugate, Au-DNA-Au nanostructure was prepared. The Au-DNA-Au nanostructure was characterized by AFM.
Pan
Fenggang
F
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Sumaoka
Jun
J
Hirano
Toshiyuki
T
Okutsu
Yoshitaka
Y
Takeuchi
Osamu
O
Shigekawa
Hidemi
H
Komiyama
Makoto
M
D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force
D015342:DNA Probes; D006046:Gold; D004247:DNA