One-step generation of recombineering constructs by asymmetric-end ligation and negative selection.
Ohtsuka
Masato
M
Division of Basic Molecular Science and Molecular Medicine, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa 259-1193, Japan. masato@is.icc.u-tokai.ac.jp
Mizutani
Akiko
A
Kikuti
Yukie Y
YY
Kulski
Jerzy K
JK
Sato
Masahiro
M
Kimura
Minoru
M
Tanaka
Masafumi
M
Inoko
Hidetoshi
H
D003001:Cloning, Molecular; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins