title: Characterization of Salmonella enterica serovar Typhimurium DT104 invasion in an epithelial cell line (IPEC J2) from porcine small intestine.
abstract: Salmonella Typhimurium DT104 is an emerging enteric pathogen in swine of increasing medical importance. In this study, the time course and the actin-dependent host signaling processes necessary for invasion of a S. Typhimurium DT104 field isolate were investigated in IPEC J2 epithelial cells derived from porcine small intestine. Internalized bacteria were quantified by a gentamicin resistance assay. DT104 internalization into epithelial monolayers increased steadily between 15 and 120min after apical inoculation. Internalization was reduced by the Rho GTPase inhibitor mevastatin, the N-WASP inhibitor wiskostatin and the actin-disrupting agent cytochalasin D, but not the Rac1 GTPase inhibitor NSC-23766. Early DT104 invasion of porcine enterocytes appears to be mediated by Rac1 GTPase-independent changes in epithelial actin assembly.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Minnesota, St Paul, MN 55108-6010, USA. brown013@umn.edu
authors.forename: David R
authors.initials: DR
authors.lastname: Brown
authors.forename: Lisa D
authors.initials: LD
authors.lastname: Price
mesh_terms: D000818:Animals; D002227:Carbazoles / Q000494:pharmacology; D002460:Cell Line; D015169:Colony Count, Microbial; D015638:Cytochalasin D / Q000494:pharmacology; D017097:Electric Impedance; D004847:Epithelial Cells / Q000382:microbiology; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology; D007421:Intestine, Small / Q000166:cytology / Q000382:microbiology*; D008148:Lovastatin / Q000031:analogs & derivatives / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011412:Propanolamines / Q000494:pharmacology; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000502:physiology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D013997:Time Factors
chemical_list: D002227:Carbazoles; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; D011412:Propanolamines; C489942:wiskostatin; C012258:mevastatin; D015638:Cytochalasin D; D008148:Lovastatin