Synthesis and hybridization studies on oligonucleotide-metal complex conjugates.
We synthesized a DNA oligomer with a terpyridine x Cu(II) complex-linked thymidine residue at phosphors the 5'-end. The attachment of the 5'-complex slightly enhanced the thermal stability of the DNA match-duplex. We also determined the thermal stabilities of the modified DNA duplexes and their unmodified counterparts, with a mismatched base-pair, to examine the effect of the complex on the stability.
Hara
Kazuhiro
K
Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585,Japan.
Kitamura
Masaya
M
Inoue
Hideo
H
D020137:Base Pair Mismatch; D001483:Base Sequence; D008670:Metals / Q000737:chemistry*; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D013816:Thermodynamics; D013936:Thymidine / Q000737:chemistry; D044366:Transition Temperature
D008670:Metals; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; D011725:Pyridines; D013936:Thymidine