Efficient generation of 2'-deoxyuridin-5-yl at 5'-(G/C)AA(X)U(X)U-3' (X = Br, I) sequences in duplex DNA under UV-irradiation.
A highly sequence-specific photoreaction of 5'-(G/C)AA(X)U(X)U-3' was found by using 450-base pair DNA fragments containing (X)U under UV-irradiation (X = Br, I). The molecular basis of this high photoreactivity was investigated in detail using synthetic octanucleotides. Based on the experimental data, the mechanism for the efficient photoreaction was proposed.
Watanabe
Takayoshi
T
School of Biomedical Science, Tokyo Medical and Dental University, 2-3-10 Surugadai, Kanda, Chiyoda, Tokyo 101-0062, Japan.
Bando
Toshikazu
T
Xu
Yan
Y
Sugiyama
Hiroshi
H
D001483:Base Sequence; D001966:Bromine / Q000737:chemistry*; D004247:DNA / Q000138:chemical synthesis* / Q000235:genetics / Q000528:radiation effects*; D007455:Iodine / Q000737:chemistry*; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000138:chemical synthesis* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014529:Uridine / Q000138:chemical synthesis*
D009692:Nucleic Acid Heteroduplexes; D004247:DNA; D007455:Iodine; D001966:Bromine; D014529:Uridine