Reversible DNA photoligation via alpha-5-cyanovinyldeoxyuridine.
An efficient and reversible Template-directed Photoligation of oligodeoxynucleotides (ODNs) using alpha-5-cyanovinyldeoxyuridine (alpha-(CNV)U) was described. When ODN containing alpha-(CNV)U at the 3' end was photoirradiated with ODN containing thymidine at the 5' end in the presence of a template ODN, an efficient photoligation was observed. The ligated ODN can be split site-selectively to regenerate parent ODN by photoirradition at the 312 nm.
Ogino
Masayuki
M
The School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa 923-1292, Japan.
Yoshimura
Yoshinaga
Y
Nakazawa
Akio
A
Saito
Isao
I
Fujimoto
Kenzo
K
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects*; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D008027:Light; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000032:analysis / Q000737:chemistry / Q000528:radiation effects
C509569:5-cyanovinyldeoxyuridine; D009838:Oligodeoxyribonucleotides; D004247:DNA; D003857:Deoxyuridine