title: Administering cultured Taxus cells with early precursors reveals bifurcations in the taxoid biosynthetic pathway.
abstract: Administering Taxus suspension cells with labeled 5alpha-hydroxytaxadiene and 5alpha,10beta-dihydroxytaxadiene, and the corresponding 5alpha-acetate esters, demonstrated that acetylation at C5 of the monool precursor promotes the formation of 14beta-hydroxy taxoids, such as taxuyunnanine C, at the expense of 13alpha-hydroxy taxoids, including Taxol and its congeners, but that the major bifurcation in taxoid biosynthesis, toward 13alpha- or 14beta-hydroxy taxoids, occurs after 10beta-hydroxylation of the taxane core.
authors.affiliation: Institute of Biological Chemistry, Washington State University, 299 Clark Hall, P.O. Box 646340, Pullman, WA 99164-6340, USA. rketchum@wsu.edu
authors.forename: Raymond E B
authors.initials: RE
authors.lastname: Ketchum
authors.forename: Tohru
authors.initials: T
authors.lastname: Horiguchi
authors.forename: Deyou
authors.initials: D
authors.lastname: Qiu
authors.forename: Robert M
authors.initials: RM
authors.lastname: Williams
authors.forename: Rodney B
authors.initials: RB
authors.lastname: Croteau
mesh_terms: D002478:Cells, Cultured; D043823:Taxoids / Q000378:metabolism*; D020946:Taxus / Q000166:cytology / Q000378:metabolism*
chemical_list: D043823:Taxoids