Myogenesis in hemopoietic tissue mesenchymal stem cell culture.
The myogenic differentiation capacity of prenatal mesenchymal stem cells from the main sites of hemopoiesis (bone marrow, thymus, liver, and spleen) was studied. Myogenesis was observed in all studied cell cultures except splenic mesenchymal stem cells. Differentiating cells from the thymus, bone marrow, and liver were positively stained for skeletal muscle markers (myogenin and MyoD). Autonomously contracting structures positively stained for cardiotroponin I and slow muscle myosin, were detected in the same cultures. Our experiments revealed differences in differentiation of mesenchymal stem cells from hemopoietic organs depending on the source of cells.
Gornostaeva
S N
SN
Institute of Stem Cell and Cell Technologies, Moscow. sgen@mail.ru
Rzhaninova
A A
AA
Gol'dstein
D V
DV
D001374:Azacitidine / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015316:Genetic Therapy / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D024510:Muscle Development; D009218:Myosins / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology; D014336:Troponin / Q000378:metabolism
D004791:Enzyme Inhibitors; D014336:Troponin; D009218:Myosins; D001374:Azacitidine