Translesion synthesis by human DNA polymerase eta across oxidative products of guanine.
Guanine is the most oxidizable base among natural bases. 8-Oxoguanine (8-oxoG) is the typical oxidative product, but the amount of 8-oxoG does not directly reflect the strength of oxidative stress. Imidazolone, oxazolone and guanidinohydantoin are oxidative products of guanine and 8-oxoG. Here, we investigated enzymatic reactions with human DNA polymerase eta on these lesions.
Kino
Katuhito
K
Cellular Physiology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Ito
Nobutoshi
N
Sugasawa
Kaoru
K
Sugiyama
Hiroshi
H
Hanaoka
Fumio
F
D001483:Base Sequence; D004249:DNA Damage; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006147:Guanine / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism; D010084:Oxidation-Reduction; D013379:Substrate Specificity; D000099270:Y-Family DNA Polymerases
D004259:DNA-Directed DNA Polymerase; D006147:Guanine; D009711:Nucleotides; C112338:Rad30 protein; D000099270:Y-Family DNA Polymerases