Development of triplex formation-based artificial transcription factor to recognize any upstream sequence of target genes.
Triplex formation-based artificial transcription factor to recognize any upstream sequence of desired genes was developed to regulate expression of any target genes. The reporter beta-galactosidase activity in yeast was increased 1.5-2 times by the introduction of the artificial transcription factor. The novel factor may be a useful tool to regulate expression of any target genes and reveal unknown function of the target genes.
Torigoe
Hidetaka
H
Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan.
Tsukamoto
Yusuke
Y
D000426:Alcohol Dehydrogenase / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry; D016601:RNA-Binding Proteins / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D050436:Regulatory Elements, Transcriptional; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015003:Yeasts / Q000235:genetics; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C051802:triplex DNA; D012313:RNA; D004247:DNA; D000426:Alcohol Dehydrogenase; D001616:beta-Galactosidase