Glucocorticoid receptor subunit gene expression in parotid gland and adenomas.
OBJECTIVE
The present study was undertaken to investigate the expressions of glucocorticoid receptor-alpha (GR-alpha) and -beta (GR-beta) messenger RNA (mRNA) in normal parotid and adenoma tissues.


METHODS
Eighteen pleomorphic adenomas of the parotid gland and 12 parotid gland tissues adjacent to adenomas were studied by using real-time fluorescent quantitative reverse-transcription polymerase chain reaction method.


RESULTS
The expression of both GR-alpha and GR-beta mRNA in parotid adenoma were higher than that in normal parotid glands (P<0.001), the GR-alpha/GR-beta ratios in parotid adenoma were lower than that in normal parotid glands (P<0.001), and there were no differences of both GR-alpha and GR-beta mRNA as well as GR-alpha/GR-beta ratios in male and female (P>0.05).


CONCLUSIONS
Our results showed that the mRNA expression of both GR-alpha and GR-beta were detectable in all studied specimens. The mRNA levels of these 2 GRs were higher, whereas the GR-alpha/GR-beta ratios were lower in adenomas tissues than that in the parotid gland; no differences of these 2 GRs as well as GR-alpha/GR-beta ratios were found between sexes. These data indicate that the relationship between the expressions of GRs and the clinical significance in parotid adenomas need further and profound investigation.
Zhang
Xiao-Wen
XW
Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated Hospital, Sun Yat-Sen University, Guangdong, PR China. zyzdd7755@sina.com.cn
Li
Yuan
Y
Liu
Jia-Jun
JJ
Liu
Xian
X
Wang
Zhen-Lin
ZL
Hu
Bin
B
D008949:Adenoma, Pleomorphic / Q000235:genetics* / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D010306:Parotid Gland / Q000378:metabolism*; D010307:Parotid Neoplasms / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; C411470:glucocorticoid receptor alpha; C121943:glucocorticoid receptor beta