title: Overexpression of Dicer enhances RNAi-mediated gene silencing by short-hairpin RNAs (shRNAs) in human cells.
abstract: RNA interference (RNAi) is a process in which small-interfering RNA (siRNA) induces a sequence-dependent degradation of mRNA. In recent years, vector-based siRNA expression systems have been developed by several groups. In these systems, short-hairpin RNAs (shRNAs) have been widely chosen for first transcriptional products which are processed to siRNAs by Dicer-dependent cleavage. In this study, we demonstrate that overexpression of Dicer enhances the effect of shRNA-dependent gene silencing in mammalian cells. Therefore, these results indicate that Dicer-dependent cleavage of shRNAs is an important step for shRNA-mediated gene silencing.
authors.affiliation: Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba Science City 305-8562, Japan.
authors.forename: Toshiyasu
authors.initials: T
authors.lastname: Mikuma
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Kazunari
authors.initials: K
authors.lastname: Taira
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000201:enzymology*; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008954:Models, Biological; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D043244:Ribonuclease III / Q000235:genetics* / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D012343:RNA, Transfer; D008156:Luciferases; D043244:Ribonuclease III