title: [Association of fibrinogen B beta -1420G/A, -993C/T and -854G/A gene polymorphism with coronary heart disease].
abstract: OBJECTIVE
  To analyze the frequency of FGB gene -1420G/A, -993C/T and -854G/A polymorphisms, and their association with plasma fibrinogen levels in patients with coronary heart disease and in health adults.
  
  
  METHODS
  The FGB gene -1420G/A, -993C/T and -854G/A polymorphisms were analyzed with restriction fragment length polymorphisms, polymerase chain reaction with allele-specific primer and nucleotide sequencing methods. Plasma fibrinogen levels were determined by turbidimetry.
  
  
  RESULTS
  The frequencies of -1420A were 0.33 in patients with coronary heart disease and 0.26 in health adults. The frequencies of -1420A in coronary heart disease were apparently higher than that in health adults. There were no difference in frequencies of other two alleles. The logistic study suggested -1420G/A polymorphism was associated with coronary heart disease. There are significantly difference in plasma fibrinogen levels in two groups. Plasma fibrinogen levels were significantly increased in patients with coronary heart disease.
  
  
  CONCLUSIONS
  This study suggests -1420G/A polymorphism may be associated with occurrence of coronary heart disease.
authors.affiliation: Department of Hematology, Affiliated Hospital, Luzhou Medical College, Luzhou, Sichuan, 646000, P. R. China. xinghy52003@126.com
authors.forename: Hong-yun
authors.initials: HY
authors.lastname: Xing
authors.forename: Wang-wei
authors.initials: WW
authors.lastname: Cai
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Li
authors.forename: Qiao
authors.initials: Q
authors.lastname: Fu
authors.forename: Guo-xun
authors.initials: GX
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001483:Base Sequence; D003327:Coronary Disease / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005260:Female; D005340:Fibrinogen / Q000235:genetics* / Q000378:metabolism; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: C115499:BBeta fibrinogen; D005340:Fibrinogen; D004247:DNA