title: Presence of a lethal protease in the extracellular products of Vibrio splendidus-Vibrio lentus related strains.
abstract: The presence of a lethal extracellular 39-kDa protease, a virulence determinant of a Listonella pelagia strain which produces vibriosis in turbot, was determined in the extracellular products (ECP) of 33 Vibrionaceae strains. Both immunological and enzymatic techniques distinguished this specific protease from other Vibrionaceae proteins. It was detected in 15% (5/33) of the ECPs assayed belonging to strains of the Vibrio splendidus-V. lentus related group isolated in Galician aquaculture systems (NW Spain). As these strains were associated with diseased octopus and cultured turbot, were able to colonize the internal organs of fish and produced a lethal ECP for fish, they are a potential risk for the health of reared aquatic organisms.
authors.affiliation: Departamento de Biologia Funcional y Ciencias de la Salud, Facultad de Biologia, Universidad de Vigo, Vigo, Spain. rfarto@uvigo.es
authors.forename: R
authors.initials: R
authors.lastname: Farto
authors.forename: S P
authors.initials: SP
authors.lastname: Armada
authors.forename: M
authors.initials: M
authors.lastname: Montes
authors.forename: M J
authors.initials: MJ
authors.lastname: Perez
authors.forename: T P
authors.initials: TP
authors.lastname: Nieto
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification; D015153:Blotting, Western; D002364:Caseins / Q000037:antagonists & inhibitors / Q000378:metabolism; D005110:Extracellular Space / Q000201:enzymology*; D005412:Flatfishes; D010447:Peptide Hydrolases / Q000008:administration & dosage / Q000302:isolation & purification*; D013030:Spain; D014733:Vibrio / Q000145:classification / Q000201:enzymology / Q000472:pathogenicity*; D014735:Vibrio Infections / Q000401:mortality / Q000517:prevention & control; D014774:Virulence
chemical_list: D000942:Antigens, Bacterial; D002364:Caseins; D010447:Peptide Hydrolases