A metalloproteinase gene from the pathogenic piscine hemoflagellate, Cryptobia salmositica.
We report the identification of a Cryptobia genomic DNA gene predicted to encode a hydrophobic protein containing a zinc metalloproteinase motif, HEXXH, and hence named it a major surface proteinase 1-like (MSP-1). The MSP-1 gene was identified using universal genome walking. Southern blot analysis revealed it to be a multicopy gene. Fragments of DNA encoding a segment homologous to the HEXXH motif of MSP-1 are widely found in bacteria, yeast, parasitic protozoans, plants, and animals including humans. These results suggest that the MSP-1 may have highly conserved functions, such as in intracellular proteolysis.
Jesudhasan
Palmy R
PR
Department of Integrative Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada. jesudhasan@poultry.tamu.edu
Tan
Chung-Wei
CW
Woo
Patrick T K
PT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D056890:Eukaryota / Q000201:enzymology* / Q000235:genetics*; D005399:Fishes / Q000469:parasitology; D045726:Metalloproteases / Q000235:genetics*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D015800:Protozoan Proteins; D045726:Metalloproteases