Micropatterning of cell-repellent polymer on a glass substrate for the highly resolved virus microarray.
The development of a simple and easily accessible method to control cellular behavior under a spatially controlled surface is critical for fundamental studies in biotechnology. We fabricated a microarray of Spodoptera frugiperda 9 (Sf9) cells on a glass surface by microcontact printing cell-repellent polymeric molecules of poly(ethylene glycol)-branched-poly(methyl methacrylate) as a template for cell micropatterning. The polymer micropatterns enabled the stable confinement of Sf9 cells on the surface, resulting in the formation of a cell microarray. Subsequently, the patterned Sf9 cells were infected with recombinant baculovirus modified with green fluorescent protein (GFP) to form a virus microarray, and GFP expression in the virus microarray was verified with confocal fluorescence microscopy.
Na
Kyunga
K
Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Korea.
Jung
Jaeyeon
J
Shin
Byungcheol
B
Hyun
Jinho
J
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002460:Cell Line; D005898:Glass / Q000737:chemistry; D046228:Microarray Analysis / Q000379:methods; D018613:Microscopy, Confocal; D011092:Polyethylene Glycols / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D018411:Spodoptera / Q000166:cytology* / Q000235:genetics / Q000821:virology
D011092:Polyethylene Glycols; D019904:Polymethyl Methacrylate