[The influences of lactose as an inducer on expression of plant des-pGlu1-brazzein in Escherichia coli].
Expression strain of des-pGlu1-brazzein was constructed and the conditions using lactose as inducer was also optimized. The Influences of three factors which were lactose concentration, induction time and inducing temperature on the growth of strain and on the yield of des-pGlul-Brazzein was analyzed in detail. The result indicated that high lactose concentration inhibit the growth of strains (P < 0.01) but made no difference on expression of target protein between 0.5%-5% (P > 0.05), Biomass would be improved as time passed (P < 0.01), but the yield of target protein didn't increase obviously at 30 degrees C compared with at 37 degrees C. Further result showed that the greater expressed level of des-pGlul-Brazzein, as high as about 20% of total cell protein, could be achieved after the strain had been induced with 0.5% lactose under 28 degrees C - 30 degrees C for 4 h.
Li
Chun-Li
CL
College of Animal and Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China. hnclli@163.com
Chen
Qi-Xin
QX
He
Guo-Qing
GQ
D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D007785:Lactose / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D013696:Temperature; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects*
D010940:Plant Proteins; C090521:brazzein protein, Pentadiplandra brazzeana; D007544:Isopropyl Thiogalactoside; D007785:Lactose