Organization of a partial S10 operon and its transcriptional analysis in Flavobacterium hibernum strain W22.
A cluster of six genes from Flavobacterium hibernum strain W22, fus-rpsJ-rplC-rplD-rplW-rplB, was cloned and sequenced. A short fragment upstream of rplC, but not of rpsJ, showed strong promoter activity in flavobacteria. TATCTTTG and TTG motifs that are conserved in Flavobacterium promoters were found immediately upstream of the transcription start point of rplC, at the -7 and -33 positions, respectively. RT-PCR analysis of the transcripts revealed that rpsJ and rplC are expressed as separate transcriptional units, whereas rplC and rplD-rplW-rplB are cotranscribed as a single mRNA, in contrast to the situation in the Gammaproteobacteria, which have a single transcriptional unit of 11 ribosomal S10 genes.
Chen
Shicheng
S
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
Bagdasarian
Michael
M
Kaufman
Michael G
MG
Walker
Edward D
ED
D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005417:Flavobacterium / Q000235:genetics*; D015870:Gene Expression; D023061:Gene Order; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins