[8-hydroxy-2'-deoxyguanosine and lipid peroxidation in patients with heart failure].
OBJECTIVE
Heart failure is associated with increased free radical production, which leads to a state of oxidative stress. Known markers of oxidative stress include 8-hydroxy-2'-deoxyguanosine, which reflects oxidative damage to DNA, and lipid peroxidation, which can be used to quantify damage to lipid-rich structures. The aims of this study were to compare 8-hydroxy-2'-deoxyguanosine and lipid peroxidation levels in heart failure patients and healthy subjects and to assess how these levels are influenced by heart failure etiology.


METHODS
The study included 78 patients (57 male, age 64 [14] years) with heart failure and 12 control subjects. Patients completed a questionnaire and were graded according to the New York Heart Association classification. Doppler echocardiography was performed and blood samples were obtained. 8-hydroxy-2'-deoxyguanosine and lipid peroxidation levels were determined.


RESULTS
Significant differences were observed between patients and control subjects in 8-hydroxy-2'-deoxyguanosine and lipid peroxidation levels, at 0.34 (0.54) ng/mL vs 0.04 (0.07) ng/mL (P<.05), and 18 (10) microM vs 8 (3) microM (P<.01), respectively. Subsequent analysis showed that heart failure etiology had a significant effect on the levels of the two markers (P<.05), which were highest in patients with hypertensive cardiomyopathy.


CONCLUSIONS
Levels of 8-hydroxy-2'-deoxyguanosine and lipid peroxidation were higher in heart failure patients than in control subjects. The most significant increases were found in patients with hypertensive cardiomyopathy.
Rivera
Miguel
M
Unidad de Cardiología, Centro de Investigación Hospital La Fe. Valencia, Spain. rivera_jmi@gva.es
Roselló-Lletí
Esther
E
García de Burgos
Fernando
F
Bertomeu
Vicente
V
Payá
Rafael
R
Cortés
Raquel
R
Martínez-Dolz
Luís
L
Jordán
Alejandro
A
Pérez-Boscá
Jose L
JL
Salvador
Antonio
A
Marín
Francisco
F
Sogorb
Francisco
F
Valero
Ricardo
R
Miró
Vicente
V
Portolés
Manuel
M
D000080242:8-Hydroxy-2'-Deoxyguanosine; D016022:Case-Control Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D006333:Heart Failure / Q000097:blood* / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine