Lipid raft proteome of the human neutrophil azurophil granule.
Detergent-resistant membrane domains (DRMs) are present in the membranes of azurophil granules in human neutrophils (Feuk-Lagerstedt et al., J. Leukoc. Biol. 2002, 72, 970). Using a proteomic approach, we have now identified 106 proteins in a DRM preparation from these granule membranes. Among these proteins were the lipid raft structural proteins flotillin-1 and -2, cytoskeletal proteins such as actin, vimentin and tubulin, and membrane fusion promoting proteins like annexins and dysferlin. Our results suggest that the azurophil granule membrane, in similarity to the plasma membrane, is an elaborate structure that takes part in intracellular signaling and functions other than the mere delivery of bactericidal effector molecules to the phagosome.
Feuk-Lagerstedt
Elisabeth
E
School of Engineering, University College of Borås, Borås, Sweden. elisabeth.feuk@rheuma.gu.se
Movitz
Charlotta
C
Pellmé
Sara
S
Dahlgren
Claes
C
Karlsson
Anna
A
D001399:Azure Stains; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D021962:Membrane Microdomains / Q000737:chemistry / Q000502:physiology*; D009504:Neutrophils / Q000737:chemistry / Q000502:physiology*; D020543:Proteome / Q000737:chemistry / Q000502:physiology*
D001399:Azure Stains; D020543:Proteome