Linkage maps for the Pacific abalone (genus Haliotis) based on microsatellite DNA markers.
This study presents linkage maps for the Pacific abalone (Haliotis discus hannai) based on 180 microsatellite DNA markers. Linkage mapping was performed using three F1 outbred families, and a composite linkage map for each sex was generated by incorporating map information from the multiple families. A total of 160 markers are placed on the consolidated female map and 167 markers on the male map. The numbers of linkage groups in the composite female and male maps are 19 and 18, respectively; however, by aligning the two maps, 18 linkage groups are formed, which are consistent with the haploid chromosome number of H. discus hannai. The female map spans 888.1 cM (Kosambi) with an average spacing of 6.3 cM; the male map spans 702.4 cM with an average spacing of 4.7 cM. However, we encountered several linkage groups that show a high level of heterogeneity in recombination rate between families even within the same sex, which reduces the precision of the consolidated maps. Nevertheless, we suggest that the composite maps are of significant potential use as a scaffold to further extend the coverage of the H. discus hannai genome with additional markers.
Sekino
Masashi
M
Tohoku National Fisheries Research Institute, Fisheries Research Agency, Shiogama, Miyagi 985-0001, Japan. sekino@affrc.go.jp
Hara
Motoyuki
M
D000818:Animals; D002874:Chromosome Mapping; D020090:Chromosome Segregation / Q000235:genetics; D016000:Cluster Analysis; D005260:Female; D049851:Gastropoda / Q000235:genetics*; D018740:Genetic Heterogeneity; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D009792:Oceans and Seas; D011995:Recombination, Genetic / Q000235:genetics
D005819:Genetic Markers