title: KlADH3, a gene encoding a mitochondrial alcohol dehydrogenase, affects respiratory metabolism and cytochrome content in Kluyveromyces lactis.
abstract: A Kluyveromyces lactis strain, harbouring KlADH3 as the unique alcohol dehydrogenase (ADH) gene, was used in a genetic screen on allyl alcohol to isolate mutants deregulated in the expression of this gene. Here we report the characterization of some mutants that lacked or had highly reduced amounts of KlAdh3p activity; in addition, these mutants showed alterations in glucose metabolism, reduced respiration and reduced cytochrome content. Our results confirm that the KlAdh3p activity contributes to the reoxidation of cytosolic NAD(P)H feeding the respiratory chain through KlNdi1p, the mitochondrial internal transdehydrogenase. The low levels of KlAdh3p in two of the mutants were associated with mutations in KlSDH1, one of the genes of complex II, suggesting signalling between the respiratory chain and expression of the KlADH3 gene.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma, La Sapienza, Rome, Italy. michele.saliola@uniroma1.it
authors.forename: Michele
authors.initials: M
authors.lastname: Saliola
authors.forename: Ilaria
authors.initials: I
authors.lastname: De Maria
authors.forename: Tiziana
authors.initials: T
authors.lastname: Lodi
authors.forename: Alessandro
authors.initials: A
authors.lastname: Fiori
authors.forename: Claudio
authors.initials: C
authors.lastname: Falcone
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D003600:Cytosol / Q000737:chemistry; D004579:Electron Transport; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D009249:NADP / Q000378:metabolism
chemical_list: D003580:Cytochromes; D045222:Electron Transport Chain Complex Proteins; D024101:Mitochondrial Proteins; D009249:NADP; D000426:Alcohol Dehydrogenase