title: Screening of DNA aptamers against multiple proteins in tissue.
abstract: We report the development of a novel screening method for DNA aptamers against multiple proteins in the target tissue. Although a purified single protein is generally used in screening of aptamers at present, such a simultaneous selection would be very advantageous in efficiency and selectivity. We first carried out the screening in situ and it was suggested that aptamers against particular target proteins were enriched. We also developed another novel screening method named Aptamer Blotting based on protein separation by PAGE and its visualization with fluorescein labeled aptamers and it was demonstrated that this method allowed the simultaneous selection of specific aptamers against multiple proteins.
authors.affiliation: Department of Biotechnology & Life Science, Tokyo University of Agriculture & Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Noma
authors.forename: Kazunori
authors.initials: K
authors.lastname: Ikebukuro
authors.forename: Koji
authors.initials: K
authors.lastname: Sode
authors.forename: Takuya
authors.initials: T
authors.lastname: Ohkubo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Sakasegawa
authors.forename: Naomi
authors.initials: N
authors.lastname: Hachiya
authors.forename: Kiyotoshi
authors.initials: K
authors.lastname: Kaneko
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000032:analysis*; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D015151:Immunoblotting / Q000379:methods*; D015336:Molecular Probe Techniques; D018482:Muscle, Skeletal / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D011506:Proteins; D016650:Fluorescein-5-isothiocyanate