[The expression of HIF-1 in the early diabetic NOD mice].
OBJECTIVE
To study the retinal expression of HIF-1 alpha in the diabetic NOD mice.


METHODS
NOD mice were divided into normal control group and diabetes group randomly, sacrificed at 2, 4, 6, 8 or 12 weeks. Eyes were enucleated and retinas were isolated and used for western blot, or eyes were sectioned for immunohistochemical staining for HIF-1 alphal.


RESULTS
Compared to the normal control group, blood sugar of diabetes mice increased significantly but the weights decreased significantly (P < 0.01). Western blot of retinal lysates showed low levels of HIF-1 alpha at the 6th weeks that were markedly increased at the 12th weeks in the diabetes group. Immunohistochemistry showed increased staining for HIF-1 in the inner retina, but not in the outer retina.


CONCLUSIONS
The retinal HIF-1 alpha levels increasing showed that retinal ischemia happening in the early diabetes. HIF-1 alpha may participate in the secondary damage of retinal ischemia induced by early diabetes.
Li
Cairui
C
Department of Ophthalmology, Xiangya Second Hospital of Central South University, Changsha 410007, China.
Xu
Yan
Y
Jiang
Deyong
D
Hong
Wei
W
Guo
Xiaojian
X
Wang
Ping
P
Li
Wengling
W
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D003930:Diabetic Retinopathy / Q000378:metabolism; D005260:Female; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis*; D051379:Mice; D016688:Mice, Inbred NOD; D012160:Retina / Q000378:metabolism* / Q000473:pathology
D051795:Hypoxia-Inducible Factor 1, alpha Subunit