An empirical approach for thermal stability (Tm) prediction of PNA/DNA duplexes.
To predict the Tm value of PNA/DNA duplex, we performed to determine the melting temperature of several PNA oligomers. For Tm calculation model building, we searched the correlativity of measured Tm value and each base contents of PNA oligomer. Here, We propose a novel formula for thermal stability (Tm) prediction of PNA/DNA duplexes.
Takiya
Toshiyuki
T
Nippon Flour Mills Co., Ltd., 5-1-3 Midorigaoka, Atsugi 243-0041, Japan.
Seto
Yasuhiro
Y
Yasuda
Hideyo
H
Suzuki
Tohru
T
Kawai
Keiichi
K
D001482:Base Composition; D004247:DNA / Q000737:chemistry*; D036262:Empirical Research; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D013816:Thermodynamics; D044366:Transition Temperature
D009692:Nucleic Acid Heteroduplexes; D020135:Peptide Nucleic Acids; D004247:DNA