Usefulness of PCR-restriction fragment length polymorphism typing of the coagulase gene to discriminate arbekacin-resistant methicillin-resistant Staphylococcus aureus strains.
We compared the results of two typing methods for 678 strains of methicillin-resistant Staphylococcus aureus and methicillin-susceptible S. aureus. PCR-restriction fragment length polymorphism typing of the coagulase gene was a more reliable method than coagulase serotyping from the viewpoint of arbekacin resistance.
Ishino
Keiko
K
Department of Bioactive Molecules, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. ishino@nih.go.jp
Tsuchizaki
Naofumi
N
Ishikawa
Jun
J
Hotta
Kunimoto
K
D000617:Aminoglycosides / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D003030:Coagulase / Q000235:genetics*; D003982:Dibekacin / Q000031:analogs & derivatives* / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D016106:Methicillin Resistance; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D013203:Staphylococcal Infections; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics
D000617:Aminoglycosides; D000890:Anti-Infective Agents; D003030:Coagulase; D003982:Dibekacin; C031986:arbekacin