Nucleotide insertion opposite a cyclobutane pyrimidine dimer analogue caused from photoligation by a replicative DNA polymerase.
We previously reported an efficient and reversible template-directed photoligation using 5-carboxyvinyl-2'-deoxyuridine ((CV)U)-containing ODN at the 5'-terminal. This method forms d(T-(CV)U) as a cyclobutane pyrimidine dimer (CPD) analogue between 3'-terminal thymidine and 5'-terminal (CV)U of two oligodeoxynucleotides (ODNs). In this study, we performed PCR using a DNA template containing d(T-(CV)U). And then, we disclosed that two adenines were incorporated opposite the d(T-(CV)U).
Ogino
Masayuki
M
The School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
Okamura
Daisuke
D
Yoshimura
Yoshinaga
Y
Fujimoto
Kenzo
K
D000225:Adenine / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D046209:Electrophoresis, Microchip; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000528:radiation effects; D010777:Photochemistry; D016133:Polymerase Chain Reaction; D011740:Pyrimidine Dimers / Q000737:chemistry*; D019914:Taq Polymerase / Q000378:metabolism*; D013698:Templates, Genetic; D013936:Thymidine / Q000031:analogs & derivatives* / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; D011740:Pyrimidine Dimers; D004247:DNA; D019914:Taq Polymerase; D000225:Adenine; D013936:Thymidine; D003857:Deoxyuridine