Novel procedure for high-fidelity tendon histology.
A novel method to obtain high-quality histologic sections of rat tendon for light microscopic study is presented. This approach utilizes non-deleterious dehydrating and clearing solvents and a hydrophobic acrylic (methyl methacrylate) resin. This methodology avoids processing and microtomy artifacts common with routine paraffin wax techniques and overcomes specimen size limitations associated with hydrophilic (e.g., glycol methacrylate) resin histologic methods. These novel histologic processing techniques facilitate the reliable assessment of tendons' cellular and matrix components and can be readily adapted to morphologic studies of damage, healing, and repair.
Laudier
Damien
D
Leni & Peter W. May Department of Orthopaedics, Mount Sinai School of Medicine, New York, New York, USA.
Schaffler
Mitchell B
MB
Flatow
Evan L
EL
Wang
Vincent M
VM
D000180:Acrylic Resins; D000818:Animals; D005260:Female; D016613:Plastic Embedding / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013710:Tendons / Q000033:anatomy & histology*
D000180:Acrylic Resins