[FTIR spectroscopic characterization of freshly removed breast cancer tissues].
OBJECTIVE
To identify the FTIR spectroscopic characterization of breast cancer and explore the possibility of application of FTIR in differentiation of malignant and benign breast lesions.


METHODS
FTIR spectra of surgically removed fresh breast tissues were measured by spectrometer equipped with mid-infrared fiber optics and an ATR probe. Peaks in the spectra were measured and relative intensity ratios were calculated and analyzed if there are significant differences between the spectra of malignant and benign breast lesions.


RESULTS
There were significant differences (P < 0.05) between the spectra of malignant breast cancers and benign breast tissues in the relative intensity ratios of different peaks (I1640/ I1550 and I1160/I1120 for protein structures; I1640/I1460 and I1550/I1460 for relative content of protein and lipid; I1460/I1400 for lipid structures; I1310/I1240 for nucleic acid).


CONCLUSIONS
FTIR spectroscopy could be a useful tool in clinical diagnosis of breast cancer.
Zhou
Su
S
Department of General Surgery, Third Hospital of Peking University, Beijing 100083, China.
Xu
Zhi
Z
Ling
Xiao-Feng
XF
Li
Qing-Bo
QB
Xu
Yi-Zhuang
YZ
Zhang
Li
L
Zhao
Hong-Mei
HM
Wang
Li-Xin
LX
Hou
Kuan-Yong
KY
Zhou
Xiao-Si
XS
Wu
Jin-Guang
JG
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001940:Breast / Q000737:chemistry / Q000473:pathology; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D018270:Carcinoma, Ductal, Breast / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D018226:Fibroadenoma / Q000175:diagnosis* / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000032:analysis; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D008055:Lipids; D009363:Neoplasm Proteins