Disruption of ins-11, a Caenorhabditis elegans insulin-like gene, and phenotypic analyses of the gene-disrupted animal.
The insulin/insulin-like growth factor-I signaling (IIS) pathway regulates larval diapause, adult lifespan, fat metabolism, and stress-resistance in the nematode Caenorhabditis elegans. One of 38 C. elegans insulin-like genes, ins-11, was disrupted and phenotypic analyses of the gene-disrupted animal were performed. The gene-disruption exhibited a significant influence on the adult lifespan. It antagonized the lifespan extension induced by RNAi knockdown of another insulin-like gene, ins-7. Hence ins-11 appears to be necessary for lifespan extension caused by a decrease in the IIS pathway. This is the first description of gene-disruption of the C. elegans insulin-like gene that suppresses the lifespan extension.
Kawano
Tsuyoshi
T
Department of Biological and Environmental Chemistry, Faculty of Agriculture, Tottori University. kawano@muses.tottori-u.ac.jp
Nagatomo
Ryosuke
R
Kimura
Yasuo
Y
Gengyo-Ando
Keiko
K
Mitani
Shohei
S
D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary; D007328:Insulin / Q000235:genetics*; D010641:Phenotype; D034622:RNA Interference
D017931:DNA Primers; D018076:DNA, Complementary; D007328:Insulin