[Application of SELDI-TOF MS technology analyzing serum protein profiling for distinguishing laryngeal carcinoma from ordinary people].
OBJECTIVE
To analyze the spectrometric serum protein profiling of laryngeal carcinoma and healthy controls by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS), to establish serum marker pattern for the diagnosis of laryngeal carcinoma.


METHODS
The serum samples of 46 cases of laryngeal carcinoma, 51 cases of healthy controls on WCX2 proteinchip, were collected. the spectrometric protein profiling was defected by SELDI-TOF-MS, the data were analyzed by Biomarker Patterns Software provided by Ciphergen Corp. A primary diagnosis model of laryngeal carcinoma was set up. This model was further evaluated by blind test with other l2 cases patients and 13 cases healthy controls.


RESULTS
Seventy-six protein peaks were detected at the molecular range of 2000-20000 Da., among which 27 ones were significantly different between laryngeal carcinoma and controls( P < 0.05). A diagnostic pattern consisting of 3 protein peaks was established with of accuracy 88.7% (86/97), sensitivity 87% (40/46), specificity 90.2% (46/51), Blind test generated a sensitivity of 83.3% (10/12)and specificity of 84.6% (11/13) respectively.


CONCLUSIONS
It is successful to develop and evaluate the different spectrometric protein profiling patterns of laryngeal carcinoma and healthy control by SELDI-TOF-MS. This affords possibly a new method to early diagnosis of laryngeal carcinoma.
Zhang
Xiuqiang
X
Department of Otorhinolaryngology, Li-Huili Hospital, Medical Center of Ningbo, Zhejiang, 315041, China. zhxqsd@163.com
Chen
Ying
Y
Wang
Jinguo
J
Qu
Xun
X
Deng
Biping
B
Wang
Jingzhi
J
Xie
Guang
G
Li
Mei
M
Xu
Fenglei
F
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000175:diagnosis; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor