Genome wide expression analysis of white blood cells and liver of pre-diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats using a cDNA microarray.
In a prior study, we reported on a significant decrease in calpain10 gene expression in white blood cells (WBC) as well as the major insulin-target tissues including liver and adipose tissue, before the onset of diabetes in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. In this study, we extended our hypothesis that some type 2 diabetes mellitus (NIDDM) susceptible genes are up/down-regulated before the onset in WBC of OLETF rats, reflecting their up/down-regulation in major insulin-target tissues, such as the liver. We tested this hypothesis using rat cDNA microarrays. The findings show that 1080 genes are up/down-regulated by more than 2-fold compared to the controls, Long-Evans Tokushima Otsuka rats, before the onset in WBC and liver under fasted or insulin administered condition. Fifty-seven of the 1080 genes were up/down-regulated in both WBC and the liver. More than half have been reported to NIDDM susceptible genes and the remainder have not been reported to be related to NIDDM. These results indicate that there some NIDDM related genes are up/down-regulated in WBC before the onset of diabetes.
Iida
Shinya
S
Laboratory for Molecular Design of Pharmaceutics, Graduate School of Pharmaceutical Sciences, Hokkaido University, Japan.
Sato
Yuichiro
Y
Nakaya
Akihiro
A
Shinohara
Yasuo
Y
Hayashi
Yasuhiro
Y
Sawada
Akihiro
A
Nagata
Hideya
H
Kaji
Noritada
N
Kamiya
Hiroyuki
H
Baba
Yoshinobu
Y
Harashima
Hideyoshi
H
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D018076:DNA, Complementary / Q000235:genetics*; D005215:Fasting; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D016678:Genome; D007328:Insulin / Q000008:administration & dosage; D007962:Leukocytes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011236:Prediabetic State / Q000235:genetics*; D051381:Rats; D020334:Rats, Inbred OLETF
D001786:Blood Glucose; D018076:DNA, Complementary; D007328:Insulin