Single molecule imaging of mRNA splicing.
Most eukaryotic mRNA precursors (pre-mRNAs) are interrupted by non-coding intervening sequences known as introns which must be precisely and efficiently removed to yield correct mature mRNAs. The process of intron excision, pre-mRNA splicing, takes place in a multicomponent ribonucleoprotein complexes which assemble in a stepwise pathway. Extensive studies, both genetic and biochemical, have revealed that numerous protein factors function to achieve fidelity in splicing, however the details of this process is poorly understood. We have developed a splicing assay system using the single molecule imaging technique to study the kinetics of the splicing reaction.
Ishihama
Yo
Y
Graduate School of Science and Engineering, Waseda University, Shinjyuku-ku, Tokyo 169-8555, Japan.
Tadakuma
Hisashi
H
Tani
Tokio
T
Funatsu
Takashi
T
D011088:DNA Ligases; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008856:Microscopy, Fluorescence; D012322:RNA Precursors / Q000032:analysis* / Q000378:metabolism; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*
D012322:RNA Precursors; D012333:RNA, Messenger; D011088:DNA Ligases