title: Antiproliferative effect of Erycibe elliptilimba on human breast cancer cell lines.
abstract: Erycibe elliptilimba Merr. & Chun., family Convolvulaceae, is a Thai traditional medicine which has long been prescribed for various infectious and malignant diseases. Bio-assays of extracts from Erycibe elliptilimba Merr. & Chun. showed that a fraction (fraction 3) from an methanolic extract had an antiproliferative effect on SKBR3 and MDA-MB435 human breast cancer cells. The ED50 value of Erycibe elliptilimba Merr. & Chun. fraction 3 was 56.07 and 30.61 microg/ml for SKBR3 and MDA-MB435, respectively. After 48 h of exposure, this fraction at a concentration of 100 microg/ml significantly reduced cell proliferation in both cancer cells. In MDA-MB435 cells, cell cycle analysis showed that the herb extract fraction 3 induced the accumulation of cells in G2/M phase, whereas no significant change in cell cycle was detected in SKBR3 cells. The results indicated that the extract fraction 3 could induce cell cycle arrest in some way. However, further investigation is needed to assess the molecular mechanisms mediated anticancer activities of this plant.
authors.affiliation: Department of Clinical Pathology, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. sitkm@mahidol.ac.th
authors.forename: Tanawan
authors.initials: T
authors.lastname: Kummalue
authors.forename: Pornchai
authors.initials: P
authors.lastname: O-charoenrat
authors.forename: Weena
authors.initials: W
authors.lastname: Jiratchariyakul
authors.forename: Monraudee
authors.initials: M
authors.lastname: Chanchai
authors.forename: Kovit
authors.initials: K
authors.lastname: Pattanapanyasat
authors.forename: Kasama
authors.initials: K
authors.lastname: Sukapirom
authors.forename: Surat
authors.initials: S
authors.lastname: Iemsri
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D027722:Convolvulaceae / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008518:Medicine, East Asian Traditional; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D013785:Thailand
chemical_list: D010936:Plant Extracts