title: Electrodeposition of anchored polypyrrole film on microelectrodes and stimulation of cultured cardiac myocytes.
abstract: The electrically conducting polymer polypyrrole (PPy) was electrochemically deposited onto Pt microelectrodes on a polyimide (PI) substrate. Pre-modification of the PI surface with a self-assembled monolayer of octadecyltrichlorosilane-induced anisotropic lateral growth of PPy along the PI surface and enhanced adhesive strength of the PPy film. The lateral growth of PPy film around the electrode anchored the whole film to the substrate. External stimulation of cultured cardiac myocytes was carried out using the PPy-coated microelectrode. The myocytes on the microelectrode substrate were electrically conjugated to form a sheet, and showed synchronized beating upon stimulation. The threshold charge for effective stimulation of a 0.8 cm(2) sheet of myocytes was around 0.2 microC, roughly corresponding to a membrane depolarization of 250 mV.
authors.affiliation: Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, Aramaki-Aoba 6-6-01, Sendai 980-8579, Japan. nishizawa@biomems.mech.tohoku.ac.jp
authors.forename: Matsuhiko
authors.initials: M
authors.lastname: Nishizawa
authors.forename: Hyuma
authors.initials: H
authors.lastname: Nozaki
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kaji
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kitazume
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ishibashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Abe
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D002642:Chick Embryo; D004558:Electric Stimulation; D004595:Electroplating; D008839:Microelectrodes; D032383:Myocytes, Cardiac / Q000378:metabolism*; D011108:Polymers; D011758:Pyrroles
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole