Characterization of GATA-1(+) hemangioblastic cells in the mouse embryo.
Hemangioblasts are thought to be one of the sources of hematopoietic progenitors, yet little is known about their localization and fate in the mouse embryo. We show here that a subset of cells co-expressing the hematopoietic marker GATA-1 and the endothelial marker VE-cadherin localize on the yolk sac blood islands at embryonic day 7.5. Clonal analysis demonstrated that GATA-1(+) cells isolated from E7.0-7.5 embryos include a common precursor for hematopoietic and endothelial cells. Moreover, this precursor possesses primitive and definitive hematopoietic bipotential. By using a transgenic complementation rescue approach, GATA-1(+) cell-derived progenitors were selectively restored in Runx1-deficient mice. In the rescued mice, definitive erythropoiesis was recovered but the rescued progenitors did not display multilineage hematopoiesis or intra-aortic hematopoietic clusters. These results provide evidence of the presence of GATA-1(+) hemangioblastic cells in the extra-embryonic region and also their functional contribution to hematopoiesis in the embryo.
Yokomizo
Tomomasa
T
Institute of Basic Medical Sciences and Center for TARA, University of Tsukuba, Tsukuba, Japan.
Takahashi
Satoru
S
Mochizuki
Naomi
N
Kuroha
Takashi
T
Ema
Masatsugu
M
Wakamatsu
Asami
A
Shimizu
Ritsuko
R
Ohneda
Osamu
O
Osato
Motomi
M
Okada
Hitoshi
H
Komori
Toshihisa
T
Ogawa
Minetaro
M
Nishikawa
Shin-Ichi
S
Ito
Yoshiaki
Y
Yamamoto
Masayuki
M
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D001011:Aorta / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism*; D019070:Cell Lineage; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics; D050982:GATA1 Transcription Factor / Q000235:genetics* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D013234:Stem Cells / Q000166:cytology; D019076:Transgenes; D000099152:Cadherin 5
D015703:Antigens, CD; D015820:Cadherins; D050676:Core Binding Factor Alpha 2 Subunit; D050982:GATA1 Transcription Factor; D049452:Green Fluorescent Proteins; D017493:Leukocyte Common Antigens; D000099152:Cadherin 5; C494689:Gata1 protein, mouse; C493726:Runx1 protein, mouse