Purification of hematopoietic stem cells using the side population.
Hematopoietic stem cells (HSCs) primarily reside in bone marrow, are defined by their ability to maintain blood homeostasis, and replenish themselves through self-renewal. Although HSC purification schemes vary from laboratory to laboratory, the resulting cell populations are similar, if not the same. This chapter will discuss different enrichment methods for HSCs and provide a detailed protocol for staining HSC with Hoechst 33342 for the side population (SP).
Lin
K K
KK
Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, Texas, USA.
Goodell
Margaret A
MA
D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D000943:Antigens, Differentiation / Q000032:analysis* / Q000096:biosynthesis; D001562:Benzimidazoles; D002469:Cell Separation / Q000379:methods*; D000071063:Endoglin; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000096:biosynthesis; D042787:Receptor, TIE-2 / Q000032:analysis / Q000276:immunology; D011956:Receptors, Cell Surface / Q000032:analysis / Q000276:immunology; D013194:Staining and Labeling
D015703:Antigens, CD; D000943:Antigens, Differentiation; D001562:Benzimidazoles; C064206:ENG protein, human; D000071063:Endoglin; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D042787:Receptor, TIE-2; C017807:bisbenzimide ethoxide trihydrochloride