title: Tetraplex structure of fission yeast telomeric DNA and its unfolding by the interaction with telomeric DNA binding protein Pot1.
abstract: We compared the structure of fission yeast telomeric DNA, 4G4: d(GGGGTTAC)4, and nontelomeric DNA, 4T4: d(TTTTTTAC)4, and examined their interaction with telomeric DNA binding domain of telomeric DNA binding protein Pot1 (Pot1DBD). 4T4 did not form any higher-order structure, but 4G4 formed intramolecular folded tetraplex structure in the presence of Na+. Although Pot1DBD did not induce any significant structural change of 4T4, the intramolecular folded tetraplex structure of 4G4 was unfolded by the interaction with Pot1DBD. Pot1 may facilitate telomere elongation of telomerase by disrupting the tetraplex structure of the telomeric DNA.
authors.affiliation: Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Furukawa
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Torigoe
mesh_terms: D004247:DNA / Q000737:chemistry*; D004271:DNA, Fungal / Q000737:chemistry* / Q000378:metabolism; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry; D009690:Nucleic Acid Conformation; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D000089804:Shelterin Complex; D016615:Telomere / Q000737:chemistry* / Q000378:metabolism; D034501:Telomere-Binding Proteins / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D029702:Schizosaccharomyces pombe Proteins; D000089804:Shelterin Complex; D034501:Telomere-Binding Proteins; C499082:pot1 protein, S pombe; D006147:Guanine; D004247:DNA