title: Reversible DNA photoligation via alpha-5-cyanovinyldeoxyuridine.
abstract: An efficient and reversible Template-directed Photoligation of oligodeoxynucleotides (ODNs) using alpha-5-cyanovinyldeoxyuridine (alpha-(CNV)U) was described. When ODN containing alpha-(CNV)U at the 3' end was photoirradiated with ODN containing thymidine at the 5' end in the presence of a template ODN, an efficient photoligation was observed. The ligated ODN can be split site-selectively to regenerate parent ODN by photoirradition at the 312 nm.
authors.affiliation: The School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa 923-1292, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ogino
authors.forename: Yoshinaga
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Akio
authors.initials: A
authors.lastname: Nakazawa
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
authors.forename: Kenzo
authors.initials: K
authors.lastname: Fujimoto
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects*; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D008027:Light; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000032:analysis / Q000737:chemistry / Q000528:radiation effects
chemical_list: C509569:5-cyanovinyldeoxyuridine; D009838:Oligodeoxyribonucleotides; D004247:DNA; D003857:Deoxyuridine