title: Liquid chromatography-electrospray tandem mass spectrometric method for quantification of monensin in plasma and edible tissues of chicken used in pharmacokinetic studies: applying a total error approach.
abstract: A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for use in pharmacokinetic studies in order to determine the concentrations of monensin in plasma and edible tissues of chicken. Two sample preparations were performed, one for determining monensin concentrations in plasma using acetonitrile for protein precipitation and another one for determining monensin concentrations in muscle, liver, and fat using methanol-water followed by a clean up on a solid-phase extraction cartridge. Sample extracts were injected into the LC-MS/MS system, and a gradient elution was performed on a C18 column. Narasin was used as internal standard. The LC-MS/MS method was validated using an approach based on accuracy profiles, and applicability of the method was demonstrated for the determination of monensin in chicken plasma, muscle, liver, and fat in a pharmacokinetic study.
authors.affiliation: Veterinary Drug Residues Unit, Laboratory for the Research and Investigation of Veterinary Drugs and Disinfectants, AFSSA Fougères, BP 90203, La Haute Marche, 35133 Javené, France. e.cheneau@fougeres.afssa.fr
authors.forename: Estelle
authors.initials: E
authors.lastname: Chéneau
authors.forename: Jérôme
authors.initials: J
authors.lastname: Henri
authors.forename: Yvette
authors.initials: Y
authors.lastname: Pirotais
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Abjean
authors.forename: Brigitte
authors.initials: B
authors.lastname: Roudaut
authors.forename: Pascal
authors.initials: P
authors.lastname: Sanders
authors.forename: Michel
authors.initials: M
authors.lastname: Laurentie
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D008985:Monensin / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014018:Tissue Distribution
chemical_list: D000981:Antiprotozoal Agents; D008985:Monensin