Tuning of shell and core characteristics of chitosan-decorated acrylic nanoparticles.
The aim of the work was to develop a new family of chitosan-coated acrylic nanoparticles to increase the specificity of absorption of drugs associated given by the mucosal route. To achieve this goal, techniques of radical and anionic emulsion polymerisation of isobutylcyanoacrylate (IBCA) were used. Changes in the shell composition were made by using chitosan of different molecular weight and thiolated chitosan to modify the particle surface properties in order to vary the mucosae-nanoparticle interactions. The core was also modified by the inclusion of methyl methacrylate (MMA) as second monomer potentially able to improve the control of drug release. Finally, the labelling of nanoparticles core with a fluorophore, methacryloxyethyl thiocarbamoyl rhodamine B (Polyfluor), was successfully achieved, necessary for the in vitro and in vivo evaluation of the systems created. Results showed that nanoparticle size varied from 200 to 500 nm, depending on the molecular weight of chitosan used. Positive surface charge values were obtained in all cases. In addition, evidences of the presence of thiol groups were obtained (0.03-0.16 x 10(-3)micromol/cm(2) of nanoparticle).
Bravo-Osuna
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CNRS, UMR CNRS 8612, Faculté de Pharmacie, 5 Rue J.B. Clément, 92296 Chatenay-Malabry, France.
Ponchel
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Vauthier
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D002015:Bucrylate / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000737:chemistry*; D003487:Cyanoacrylates / Q000737:chemistry*; D004586:Electrophoresis; D004655:Emulsions; D005456:Fluorescent Dyes / Q000737:chemistry; D005544:Forecasting; D009682:Magnetic Resonance Spectroscopy; D020366:Methylmethacrylate / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry; D055672:Static Electricity; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods; D013864:Thioglycolates / Q000737:chemistry
D003487:Cyanoacrylates; D004655:Emulsions; D005456:Fluorescent Dyes; D011108:Polymers; D012235:Rhodamines; D013438:Sulfhydryl Compounds; D013864:Thioglycolates; D020366:Methylmethacrylate; D002015:Bucrylate; D048271:Chitosan