Chloroplast phosphoglycerate kinase, a gluconeogenetic enzyme, is required for efficient accumulation of Bamboo mosaic virus.
The tertiary structure in the 3'-untranslated region (3'-UTR) of Bamboo mosaic virus (BaMV) RNA is known to be involved in minus-strand RNA synthesis. Proteins found in the RNA-dependent RNA polymerase (RdRp) fraction of BaMV-infected leaves interact with the radio labeled 3'-UTR probe in electrophoretic mobility shift assays (EMSA). Results derived from the ultraviolet (UV) cross-linking competition assays suggested that two cellular factors, p43 and p51, interact specifically with the 3'-UTR of BaMV RNA. p43 and p51 associate with the poly(A) tail and the pseudoknot of the BaMV 3'-UTR, respectively. p51-containing extracts specifically down-regulated minus-strand RNA synthesis when added to in vitro RdRp assays. LC/MS/MS sequencing indicates that p43 is a chloroplast phosphoglycerate kinase (PGK). When the chloroplast PKG levels were knocked down in plants, using virus-induced gene silencing system, the accumulation level of BaMV coat protein was also reduced.
Lin
Jen-Wen
JW
Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
Ding
Min-Pey
MP
Hsu
Yau-Heiu
YH
Tsai
Ching-Hsiu
CH
D020413:3' Untranslated Regions / Q000737:chemistry / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D002736:Chloroplasts / Q000201:enzymology; D020868:Gene Silencing; D005943:Gluconeogenesis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010735:Phosphoglycerate Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D039102:Poly(A)-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D017863:Potexvirus / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism
D020413:3' Untranslated Regions; D010940:Plant Proteins; D039102:Poly(A)-Binding Proteins; D012367:RNA, Viral; D010735:Phosphoglycerate Kinase