[Electrophoretic analysis of lacrimal proteins--a diagnostic method in secondary Sjogren syndrome].
OBJECTIVE
We analysed and compared electrophoretic tear proteins patterns of healthy subjects and patients with different autoimmune diseases associated with secondary Sjogren's syndrome.


METHODS
Tears were collected using the Schirmer's method. Proteins were separated by sodium-dodecyl sulfate polyacrylamide gel electrophoresis. The lanes were stained by Coomassie blue and/or silver.


RESULTS
Lactoferrin, albumin, lipocalin and lysozyme were found to be the main components being identified using molecular weight markers.


CONCLUSIONS
Electrophoretic analysis of tear proteins patterns is a fast, reproducible and simple method which provides information about the possibility of lacrimal gland involvement in auto-immune diseases.
Cojocaru
Viorica Madalina
VM
Spitalul Clinic Dr. I. Cantacuzino, Bucuresti.
Ciurtin
Coziana
C
Puchianu
Elena
E
Anothe
Felicia
F
D000890:Anti-Infective Agents / Q000032:analysis; D001327:Autoimmune Diseases / Q000175:diagnosis; D002352:Carrier Proteins / Q000032:analysis; D015853:Cysteine Proteinase Inhibitors / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005136:Eye Proteins / Q000032:analysis; D006801:Humans; D007781:Lactoferrin / Q000032:analysis; D054835:Lipocalin 1; D009113:Muramidase / Q000032:analysis; D012859:Sjogren's Syndrome / Q000175:diagnosis* / Q000276:immunology; D013666:Tears / Q000737:chemistry*
D000890:Anti-Infective Agents; D002352:Carrier Proteins; D015853:Cysteine Proteinase Inhibitors; D005136:Eye Proteins; C518443:LCN1 protein, human; D054835:Lipocalin 1; D009113:Muramidase; D007781:Lactoferrin