Administering cultured Taxus cells with early precursors reveals bifurcations in the taxoid biosynthetic pathway.
Administering Taxus suspension cells with labeled 5alpha-hydroxytaxadiene and 5alpha,10beta-dihydroxytaxadiene, and the corresponding 5alpha-acetate esters, demonstrated that acetylation at C5 of the monool precursor promotes the formation of 14beta-hydroxy taxoids, such as taxuyunnanine C, at the expense of 13alpha-hydroxy taxoids, including Taxol and its congeners, but that the major bifurcation in taxoid biosynthesis, toward 13alpha- or 14beta-hydroxy taxoids, occurs after 10beta-hydroxylation of the taxane core.
Ketchum
Raymond E B
RE
Institute of Biological Chemistry, Washington State University, 299 Clark Hall, P.O. Box 646340, Pullman, WA 99164-6340, USA. rketchum@wsu.edu
Horiguchi
Tohru
T
Qiu
Deyou
D
Williams
Robert M
RM
Croteau
Rodney B
RB
D002478:Cells, Cultured; D043823:Taxoids / Q000378:metabolism*; D020946:Taxus / Q000166:cytology / Q000378:metabolism*
D043823:Taxoids