title: [Study on molecular epidemiology of Borna disease virus in Ningxia and vicinal regions].
abstract: OBJECTIVE
  In order to investigate the epidemics of borna disease virus (BDV) in Ningxia and its vicinal regions.
  
  
  METHODS
  p24 fragment of BDV from: (1) peripheral blood mononuclear cells (PBMC) and cerebrospinal fluid mononuclear cells (CSFMC) from 52 patients with viral encephalitis (VE) and 32 healthy donors, (2) peripheral blood mononuclear cells (PBMC) from 53 patients with depressive disorder (DD) and from 360 sheep, were examined by nested reverse transcriptase polymerase chain reaction(PCR) with fluorescence quantitative PCR. Gene sequence and amino acid sequence were analysed for positive product and the molecular epidemiologic characteristics by drawing phylogenetic trees.
  
  
  RESULTS
  The positive rate of BDV p24 in CSFMC from VE (11.54%) and in PBMC from DD 11.32% was significantly higher than that in healthy donors (0%) (P < 0.05). The phylogenetic trees indicating the genetic relationship of the p24 fragment of BDV in both sheep and VE, DD in China and was similar to the nucleotide sequence of H1766 strain in Germany.
  
  
  CONCLUSIONS
  Data indicated that the BDV infection was possibly existing in VE, DD patients and health sheep in Ningxia and its vicinal regions with confined locality which called for further study.
authors.affiliation: Department of Multiple Disease, The Affiliated Hospital of Ningxia Medical College, Yinchuan 750004, China.
authors.forename: Zhen-hai
authors.initials: ZH
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
authors.forename: Yu-Xiu
authors.initials: YX
authors.lastname: Han
authors.forename: Jun
authors.initials: J
authors.lastname: Zhan
mesh_terms: D000818:Animals; D001890:Borna Disease / Q000453:epidemiology* / Q000235:genetics; D001891:Borna disease virus / Q000235:genetics* / Q000302:isolation & purification; D002681:China / Q000453:epidemiology; D003866:Depressive Disorder / Q000821:virology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D017720:Molecular Epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012756:Sheep