Effect of polysaccharides from Ganoderma lucidum on streptozotocin-induced diabetic nephropathy in mice.
The effects of Ganoderma lucidum polysaccharides (GL-PS) on renal complication in streptozotocin-induced diabetic mice have been investigated in the present study. C57BL/6J mice were made diabetic by injection of streptozotocin and GL-PS (125 and 250 mg kg-1) was administered for 8 weeks. Body weight was monitored every week. Serum glucose, creatinine (Cr), blood urea nitrogen (BUN), triglyceride (TG) and urinary albumin excretion (UAE) were measured after 8 weeks of treatment. Glomerular size and mesangial matrix index were assayed by morphometric analysis. Renal expression of transforming growth factor-beta1 (TGF-beta1) were determined by immunochemistry. Renal malondialdehyde (MDA) level and superoxide dismutase (SOD) activities were also evaluated. GL-PS was able to reduce the serum Cr and BUN levels and UAE compared with diabetic model mice in a dose-dependent manner. Increasing serum glucose and triglyceride levels in diabetic mice could also be lowered by GL-PS. Moreover, GL-PS had the capacity to improve the renal morphometric changes and oxidative stress state of diabetic mice. In summary, GL-PS can improve the metabolic abnormalities of diabetic mice and prevent or delay the progression of diabetic renal complications.
He
C-Y
CY
Department of Pharmacology, School of Basic Medical Sciences, Peking University Health Science Centre, Beijing, 100094, China.
Li
W-D
WD
Guo
S-X
SX
Lin
S-Q
SQ
Lin
Z-B
ZB
D000419:Albuminuria / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D003928:Diabetic Nephropathies / Q000139:chemically induced* / Q000188:drug therapy*; D007150:Immunohistochemistry; D007678:Kidney Glomerulus / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000494:pharmacology / Q000627:therapeutic use*; D020940:Reishi / Q000737:chemistry*; D013311:Streptozocin / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D011134:Polysaccharides; D016212:Transforming Growth Factor beta; D014280:Triglycerides; D008315:Malondialdehyde; D013311:Streptozocin; D003404:Creatinine; D013482:Superoxide Dismutase