title: Embryonic stem cells from morula.
abstract: It has been shown that it is possible to establish human embryonic stem cell (hESC) lines from morula. Details of the aforementioned injection method of morula under blastocyst are described in this chapter. This chapter also discloses the application of simultaneous staining for two markers, TRA-2-39 and Oct-4, for characteristics of nondifferentiated hESC derived from morula and gives a method. Technical approaches of freezing morula-derived hESC are discussed.
authors.affiliation: Reproductive Genetics Institute, Chicago, Illinois, USA.
authors.forename: Nick
authors.initials: N
authors.lastname: Strelchenko
authors.forename: Yury
authors.initials: Y
authors.lastname: Verlinsky
mesh_terms: D001757:Blastomeres / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D005786:Gene Expression Regulation; D006801:Humans; D007621:Karyotyping; D008677:Metaphase; D009028:Morula / Q000166:cytology* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger