title: Clear-cut photo-regulation of the formation and dissociation of the DNA duplex by modified oligonucleotide involving multiple azobenzenes.
abstract: Multiple azobenzenes were introduced into the oligonucleotide on D-threoninol for the clear-cut photo-regulation of the formation and dissociation of DNA duplex. When azobenzenes took trans form, introduction of multiple azobenzenes (azobenzenes:nucleobases = 1:2) did not interfere with duplex formation at all compared with their native duplex. In contrast, melting temperature (Tm) uniformly decreased with the number of azobenzenes when they took cis-form. As a result, clear-cut photo-regulation of the duplex formation was attained under physiological conditions. Furthermore, nearest-neighbor parameters for these azobenzene-tethered oligonucleotides were obtained. It was found that Tms estimated from these parameters well coincided with those of measured ones.
authors.affiliation: Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Asanuma
authors.forename: Daijiro
authors.initials: D
authors.lastname: Matsunaga
authors.forename: Makoto
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D001391:Azo Compounds / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000528:radiation effects; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000528:radiation effects*; D013816:Thermodynamics
chemical_list: D001391:Azo Compounds; D009838:Oligodeoxyribonucleotides; D004247:DNA; C009850:azobenzene