title: [Investigation and clinical significance of the micrometastasis of prelaryngeal lymph nodes in the patients with glottic carcinoma].
abstract: OBJECTIVE
  To investigate the clinical significance of the micrometastasis of prelaryngeal lymph nodes in the patients with glottic carcinoma.
  
  
  METHODS
  In prelaryngeal lymph nodes from 26 patients with glottic carcinoma, cytokeratin 19 (CK19) mRNA was detected with reverse transcription polymerase chain reaction (RT-PCR), comparing with the routine histopathologic methods (HE staining).
  
  
  RESULTS
  The positive expression of CK19 mRNA in prelaryngeal lymph nodes was detected in 8 patients (30.77%). While there was no positive finding by routine methods. The difference was significant (P < 0.05). In 8 cases with stage I glottic carcinoma, no micrometastasis in the prelaryngeal lymph node was found, and in 18 cases with stage II - VA glottic carcinoma, micrometastasis was found in 8 cases (44.44%) (P < 0.05).
  
  
  CONCLUSIONS
  The detection of prelaryngeal lymph nodes micrometastasis with RT-PCR is more sensitive than with the routine histopathologic methods. The study of micrometastasis of prelaryngeal lymph nodes may provide information for the clinical management of the patients with glottic carcinoma.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, China. sunyanohns@yahoo.com.cn
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Chuanshan
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Chen
authors.forename: Wenhai
authors.initials: W
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Jiang
authors.forename: Ying
authors.initials: Y
authors.lastname: Tian
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000473:pathology*; D005260:Female; D006801:Humans; D053539:Keratin-19 / Q000235:genetics; D007822:Laryngeal Neoplasms / Q000175:diagnosis / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D053539:Keratin-19; D012333:RNA, Messenger