title: An empirical approach for thermal stability (Tm) prediction of PNA/DNA duplexes.
abstract: To predict the Tm value of PNA/DNA duplex, we performed to determine the melting temperature of several PNA oligomers. For Tm calculation model building, we searched the correlativity of measured Tm value and each base contents of PNA oligomer. Here, We propose a novel formula for thermal stability (Tm) prediction of PNA/DNA duplexes.
authors.affiliation: Nippon Flour Mills Co., Ltd., 5-1-3 Midorigaoka, Atsugi 243-0041, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takiya
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Seto
authors.forename: Hideyo
authors.initials: H
authors.lastname: Yasuda
authors.forename: Tohru
authors.initials: T
authors.lastname: Suzuki
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kawai
mesh_terms: D001482:Base Composition; D004247:DNA / Q000737:chemistry*; D036262:Empirical Research; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D013816:Thermodynamics; D044366:Transition Temperature
chemical_list: D009692:Nucleic Acid Heteroduplexes; D020135:Peptide Nucleic Acids; D004247:DNA