Transcriptional and subcellular regulation of the TRIP-Br family.
TRIP-Brs are a recently discovered set of proteins whose functions remain poorly characterized. Here we report the identification of TRIP-Br3 as a member of the TRIP-Br family along with evidence showing that TRIP-Brs interact with bromodomain-containing transcriptional cofactors PCAF, STAF65gamma, and KAP1. PCAF, a histone acetyltransferase; STAF65gamma, a protein associated with histone acetylation activity; and KAP1, a corepressor, influence the transcriptional activity of TRIP-Brs differentially. Finally, while all three TRIP-Brs are localized to the nucleus, TRIP-Br2 and TRIP-Br3 are also present in the cytoplasm through interaction with CRM1. Our results suggest that different TRIP-Brs function by interacting with a wide variety of bromodomain-containing transcriptional regulators in different subcellular locales.
Lai
I-Lu
IL
Institute of Molecular Biology, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 40227, Taiwan.
Wang
Shi-Yun
SY
Yao
Ya-Li
YL
Yang
Wen-Ming
WM
D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D008856:Microscopy, Fluorescence; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D000076183:Tripartite Motif-Containing Protein 28; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; D000092468:Bromodomain Containing Proteins
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D005231:Fatty Acids, Unsaturated; D049452:Green Fluorescent Proteins; D051548:Histone Acetyltransferases; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D000076183:Tripartite Motif-Containing Protein 28; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C515965:CDCA4 protein, human; C402464:SERTAD1 protein, human; C426827:SERTAD2 protein, human; D000092468:Bromodomain Containing Proteins; C101116:TRIM28 protein, human; C038753:leptomycin B