title: Use of pulsed-field gel electrophoresis of conserved XbaI fragments for identification of swine Salmonella serotypes.
abstract: Swine Salmonella isolates (n=674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with XbaI. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation of serotype to PFGE subtype. In addition, conserved fragments were identified within the restriction patterns that were unique to each serotype. PFGE using XbaI restriction provided a possible alternative method for screening and identifying swine Salmonella serotypes.
authors.affiliation: Department of Animal Science, Iowa State University, 11 Kildee Hall, Ames, IA 50011, USA.
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Gaul
authors.forename: Stephanie
authors.initials: S
authors.lastname: Wedel
authors.forename: Matthew M
authors.initials: MM
authors.lastname: Erdman
authors.forename: D L
authors.initials: DL
authors.lastname: Harris
authors.forename: Isabel Turney
authors.initials: IT
authors.lastname: Harris
authors.forename: Kathleen E
authors.initials: KE
authors.lastname: Ferris
authors.forename: Lorraine
authors.initials: L
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D012475:Salmonella / Q000145:classification* / Q000235:genetics*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific