The gateway to transcription: identifying, characterizing and understanding promoters in the eukaryotic genome.
Eukaryotic transcriptional regulation requires the integration of complex signals by the transcriptional promoter. Distinct sequence elements, characteristic chromatin modifications and coordinated protein-DNA interactions at these sequences constitute a transcriptional regulatory code that remains poorly understood today. Here, we review recent experimental and computational advances that have enabled the identification and analysis of transcriptional promoters on an unprecedented scale, laying a foundation for systematic determination of the transcriptional regulatory networks in eukaryotic cells. The knowledge gained from these large-scale investigations has challenged some conventional concepts of promoter structure and function, and provided valuable insights into the complex gene regulatory mechanisms in a variety of organisms.
Heintzman
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Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, Biomedical Sciences Graduate Program, 9500 Gilman Drive, La Jolla, California, 92093, USA.
Ren
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B
D000818:Animals; D005057:Eukaryotic Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D016678:Genome; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014158:Transcription, Genetic