Isolation of 151 mutants that have developmental defects from T-DNA tagging.
In order to understand the mechanisms underlying plant development, a necessary first step involves the elucidation of the functions of the genes, via the analysis of mutants that exhibit developmental defects. In this study, an activation tagging mutant library harboring 80,650 independent Arabidopsis transformants was generated in order to screen for developmental mutants. A total of 129 mutants manifesting dominant developmental abnormalities were isolated, and their T-DNA insertion loci were mapped. The activation of one or more genes adjacent to a T-DNA insertion locus was confirmed in eight dominant mutants. A gene adjacent to the right border was usually activated by the 35S enhancers. Interestingly, the transcriptional activation of multiple genes within a broad range was observed in one of the mutants, which raises the possibility that activation by the 35S enhancers was not limited strictly to a single gene. In order to gain a better understanding of sexual reproduction in higher plants, we isolated 22 mutants exhibiting defects in female gametophyte development, and determined their T-DNA insertion loci. We propose that this mutant population may prove useful in the further determination of the functions of genes that play important roles in plant development.
Ahn
Ji Hoon
JH
Plant Signaling Network Research Center, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Kim
Joonki
J
Yoo
Seong Jeon
SJ
Yoo
So Yeon
SY
Roh
Hyungmin
H
Choi
Jun-Hyuk
JH
Choi
Mi Suk
MS
Chung
Kyung Sook
KS
Han
Eun Ju
EJ
Hong
Sung Myun
SM
Jung
Sung Hye
SH
Kang
Hyo Jin
HJ
Kim
Bo Kyung
BK
Kim
Mi Duk
MD
Kim
Youn Kyung
YK
Kim
Yun Hee
YH
Lee
Hanna
H
Park
Soo Hyun
SH
Yang
Jee Hoon
JH
Yang
Jung Won
JW
Yoo
Dong-Hun
DH
Yoo
Seung Kwan
SK
Lee
Jong Seob
JS
D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics*; D035264:Flowers / Q000235:genetics; D005784:Gene Amplification; D008969:Molecular Sequence Data; D009154:Mutation; D018515:Plant Leaves / Q000235:genetics; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial; D018744:DNA, Plant; C036483:T-DNA