title: Properties and EPR studies of oligodeoxynucleotides containing 2-N-tert-butylaminoxylpurines.
abstract: Oligodeoxynucleotide (ODN; 1) containing 2-N-tert-butylaminoxyladenosine (2) was synthesized by the conventional phosphoramidite method. The thermal stability and structure of the duplex of ODN 1 and the complementary strand were elucidated by UV-melting temperature measurements and CD experiments. In phosphate buffer, ODN 1 showed a three line EPR spectrum. Upon duplex formation, the EPR spectrum of 1 changed which indicates that EPR spectra of 1 can provide information on structures of ODN.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Aso
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kaneko
authors.forename: John Walter
authors.initials: JW
authors.lastname: Mirc
authors.forename: Noboru
authors.initials: N
authors.lastname: Koga
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suemune
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011687:Purines / Q000737:chemistry; D013696:Temperature
chemical_list: C520181:2-N-tert-butylaminooxyladenosine; D009838:Oligodeoxyribonucleotides; D011687:Purines; D000241:Adenosine