title: Investigation of ferrocenyl carbodiimide (FCDI) in the modification reaction of nucleic acids.
abstract: Ferrocenyl carbodiimide (FCDI) was allowed to react with thymine or guanine bases of single stranded DNA in borate buffer (pH 9.5) at 37 degrees C for a short period of time. The reactivity of FCDI with the guanine bases was smaller than that with the thymine ones and the prolongation of the reaction time resulted in a lower yield. The reactivity for double stranded oligonucleotide was extremely decreased from that for the single stranded one. One base mismatched double stranded oligonucleotides could react with FCDI, albeit slowly. These results suggested that the reactivity of FCDI was variable depending on the kind of bases and the oligonucleotide structure.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Mukumoto
authors.forename: Takahiko
authors.initials: T
authors.lastname: Nojima
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D020137:Base Pair Mismatch; D002234:Carbodiimides / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D013941:Thymine / Q000737:chemistry
chemical_list: D002234:Carbodiimides; D004277:DNA, Single-Stranded; D005296:Ferrous Compounds; D009841:Oligonucleotides; C517870:ferrocenyl carbodiimide; D004247:DNA; D013941:Thymine