[Polymorphic analysis of two Y chromosome short tandem repeat loci in Chinese Chengdu Han population].
OBJECTIVE
To reveal the distribution of genetic polymorphism of two novel Y chromosome short tandem repeat (Y-STR) loci (DYS508, DYS516) in Chinese Chengdu Han population.


METHODS
Two Y-STR loci were amplified with PCR. The products of PCR were analyzed with polyacrylamide gel electrophoresis. Each allele of these 2 Y-STR loci was sequenced and the allele ladders were constructed. Alleles of these Y-STR loci were nominated according to recommendations of the International Society of Forensic Genetics (ISFG).


RESULTS
Six and 6 alleles were observed in loci DYS508 and DYS516 respectively. The gene diversity of DYS508 and DYS516 was 0.7242 and 0.7931 respectively. The diversity of haplotype for these Y-STR loci was 0.9397. The value of discrimination power and the exclusion chance of paternity for these Y-STR loci were same as the value of haplotype diversity.


CONCLUSIONS
Both Y-STR loci are high genetic polymorphic. They are beneficial to the test of male-female mixtures and paternity identification in forensic science.
Lin
Jing-dong
JD
Department of Pathology, College of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, 350004, P. R. China.
Huang
Ai-ming
AM
Huang
Jian-wen
JW
D044466:Asian People / Q000235:genetics*; D002681:China; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005260:Female; D053803:Forensic Genetics / Q000379:methods; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences