title: Labeling of skeletal myoblasts with a novel oxygen-sensing spin probe for noninvasive monitoring of in situ oxygenation and cell therapy in heart.
abstract: We report the labeling (internalization) of skeletal myoblasts (SMs) with a novel class of oxygen-sensing paramagnetic spin probe for noninvasive tracking and in situ monitoring of oxygenation in stem cell therapy using electron paramagnetic resonance (EPR) spectroscopy. SM cells were isolated from thigh muscle biopsies of mice and propagated in culture. Labeling of SM cells with the probe was achieved by coincubating the cells with submicron-sized (270 +/- 120 nm) particulates of the probe in culture for 48 h. The labeling had no significant effect on the viability or proliferation of the cells. The SM cells labeled with the probe were transplanted in the infarcted region of mouse hearts. The engraftment of the transplanted cells in the infarct region was verified by using MY-32 staining for skeletal myocytes. The in situ Po(2) in the heart was determined noninvasively and repeatedly for 4 wk after transplantation. The results showed significant enhancement of myocardial oxygenation at the site of cell transplant compared with untreated control. In conclusion, labeling of SM cells with the oxygen-sensing spin probe offers a unique opportunity for the noninvasive monitoring of transplanted cells as well as in situ tissue Po(2) in infarcted mouse hearts.
authors.affiliation: Center for Biomedical Electron Paramagnetic Resonance Spectroscopy and Imaging, Division of Cardiothoracic Surgery, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Sheik
authors.initials: S
authors.lastname: Wisel
authors.forename: Simi M
authors.initials: SM
authors.lastname: Chacko
authors.forename: M Lakshmi
authors.initials: ML
authors.lastname: Kuppusamy
authors.forename: Ramasamy P
authors.initials: RP
authors.lastname: Pandian
authors.forename: Mahmood
authors.initials: M
authors.lastname: Khan
authors.forename: Vijay Kumar
authors.initials: VK
authors.lastname: Kutala
authors.forename: Richard W
authors.initials: RW
authors.lastname: Burry
authors.forename: Benjamin
authors.initials: B
authors.lastname: Sun
authors.forename: Pawel
authors.initials: P
authors.lastname: Kwiatkowski
authors.forename: Periannan
authors.initials: P
authors.lastname: Kuppusamy
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D004578:Electron Spin Resonance Spectroscopy; D005434:Flow Cytometry; D006321:Heart / Q000502:physiology* / Q000503:physiopathology*; D051379:Mice; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology*; D009203:Myocardial Infarction / Q000503:physiopathology*; D009206:Myocardium / Q000166:cytology; D010100:Oxygen / Q000032:analysis*
chemical_list: D010100:Oxygen