From loops to chains: unraveling the mysteries of polyubiquitin chain specificity and processivity.
Regulated protein degradation via polyubiquitination controls almost every aspect of eukaryotic cellular biology; however, the precise mechanism by which specifically linked polyubiquitin chains are formed on target proteins as well as how the processivity of chain elongation is achieved remains a mystery. Recent work using the yeast ubiquitin ligase SCF(Cdc4) and the ubiquitin conjugating enzyme, Cdc34, has helped to answer these questions by identifying the determinants of lysine-48 specific ubiquitin chain polymerization.
Sowa
Mathew E
ME
Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA.
Harper
J Wade
JW
D008958:Models, Molecular; D010441:Peptide Chain Elongation, Translational; D025821:Polyubiquitin / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D014452:Ubiquitins / Q000378:metabolism
D011506:Proteins; D014452:Ubiquitins; D025821:Polyubiquitin