Aluminium-induced phospholipid signal transduction pathway in Coffea arabica suspension cells and its amelioration by silicic acid.
Coffee (Coffea arabica L.) is of economic importance worldwide. Its growth in organic-rich acidic soils is influenced by aluminium such that coffee yield may be impaired. Herein we have used the Al-sensitive C. arabica suspension cell line L2 to analyse the effect of two different Al species on the phosphoinositide signal transduction pathway. Our results have shown that the association of Al with coffee cells was affected by the pH and the form of Al in media. More Al was associated with cells at pH 4.3 than 5.8, whereas when Al was present as hydroxyaluminosilicates (HAS) the association was halved at pH 4.3 and unchanged at pH 5.8. Two signal transduction elements were also evaluated; phospholipase C (PLC) activity and phosphatidic acid (PA) formation. PLC was inhibited ( approximately 50%) when cells were incubated for 2 h in the presence of either AlCl(3) or Al in the form of HAS. PA formation was tested as a short-term response to Al. By way of contrast to what was found for PLC, incubation of cells for 15 min in the presence of AlCl(3) decreased the formation of PA whereas the same concentration of Al as HAS produced no effect upon its formation. These results suggest that Al is capable to exert its effects upon signal transduction as Al((aq))(3+) acting upon a mechanism linked to the phosphoinositide signal transduction pathway.
Quintal-Tun
Fausto
F
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43, No. 130, Chuburná de Hidalgo, C.P. 97200 Mérida, Yucatán, Mexico.
Muñoz-Sánchez
J Armando
JA
Ramos-Díaz
Ana
A
Escamilla-Bencomo
Armando
A
Martínez-Estévez
Manuel
M
Exley
Christopher
C
Hernández-Sotomayor
S M Teresa
SM
D000535:Aluminum / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D002460:Cell Line; D040503:Coffea / Q000187:drug effects* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D012824:Silicic Acid / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000378:metabolism
D010712:Phosphatidic Acids; D010743:Phospholipids; D012824:Silicic Acid; D015544:Inositol 1,4,5-Trisphosphate; D000535:Aluminum; D010738:Type C Phospholipases