title: Delay of leaf senescence in Medicago sativa transformed with the ipt gene controlled by the senescence-specific promoter SAG12.
abstract: We report the successfull delay of leaf senescence in Medicago sativa. A highly regenerable clone of alfalfa was transformed with the construct SAG12-IPT, an approach that has already proved efficient in other crops. Several independent transformants were obtained as determined by Southern analysis and all the transformants expressed the transgene as measured by RT-PCR. In vitro and in vivo analyses showed that SAG12-IPT plants exhibited a stay-green phenotype that has the potential to greatly improve the quantity and quality of alfalfa forage.
authors.affiliation: CNR Istituto di Genetica Vegetale, via Madonna Alta 130, Perugia, Italy.
authors.forename: Ornella
authors.initials: O
authors.lastname: Calderini
authors.forename: Tessa
authors.initials: T
authors.lastname: Bovone
authors.forename: Carla
authors.initials: C
authors.lastname: Scotti
authors.forename: Fulvio
authors.initials: F
authors.lastname: Pupilli
authors.forename: Efisio
authors.initials: E
authors.lastname: Piano
authors.forename: Sergio
authors.initials: S
authors.lastname: Arcioni
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D016922:Cellular Senescence; D002734:Chlorophyll / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D000455:Medicago sativa / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D018515:Plant Leaves / Q000201:enzymology / Q000502:physiology*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014170:Transformation, Genetic
chemical_list: D002734:Chlorophyll; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase