title: Whole genome expression profiling using DNA microarray for determining biocompatibility of polymeric surfaces.
abstract: There is an ever increasing need to find surfaces that are biocompatible for applications like medical implants and microfluidics-based cell culture systems. The biocompatibility of five different surfaces with different hydrophobicity was determined using gene expression profiling as well as more conventional methods to determine biocompatibility such as cellular growth rate, morphology and the hydrophobicity of the surfaces. HeLa cells grown on polymethylmethacrylate (PMMA) or a SU-8 surface treated with HNO3-ceric ammonium nitrate (HNO3-CAN) and ethanolamine showed no differences in growth rate, morphology or gene expression profiles as compared to HeLa cells grown in cell culture flasks. Cells grown on SU-8 treated with only HNO3-CAN showed almost the same growth rate (36 +/- 1 h) and similar morphology as cells grown in cell culture flasks (32 +/- 1 h), indicating good biocompatibility. However, more than 200 genes showed different expression levels in cells grown on SU-8 treated with HNO3-CAN compared to cells grown in cell culture flasks. This shows that gene expression profiling is a simple and precise method for determining differences in cells grown on different surfaces that are otherwise difficult to find using conventional methods. It is particularly noteworthy that no correlation was found between surface hydrophobicity and biocompatibility.
authors.affiliation: MIC-Department of Micro and Nanotechnology, Technical University of Denmark, Orsteds Plads 345 East, DK-2800, Kgs. Lyngby, Denmark. mdu@mic.dtu.dk
authors.forename: Michael
authors.initials: M
authors.lastname: Stangegaard
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: J P
authors.initials: JP
authors.lastname: Kutter
authors.forename: M
authors.initials: M
authors.lastname: Dufva
authors.forename: A
authors.initials: A
authors.lastname: Wolff
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D019856:Ethanolamine; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects*; D015894:Genome, Human / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D014867:Water; D019856:Ethanolamine