title: Antisense activity of 2',4'-BNA targeted to bcl-xL gene in HepG2 cell.
abstract: Introduction of the 2',4'-BNA monomer into oligonucleotides significantly enhanced binding affinity toward ssRNA and resistance to nuclease degradation. Here, we evaluated the antisense activity of 2',4'-BNA oligonucleotides against bcl-xL in HepG2 cells. Our data revealed that 2',4'-BNA antisense oligonucleotides remarkably inhibited the expression of bcl-xL in HepG2 cells compared to the natural DNA antisense oligonucleotide. The inhibitory effect of 2',4'-BNA antisense oligonucleotide was dose-dependent and highly sequence-specific.
authors.affiliation: Graduate School of Pharmaceutical Science, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Somjing
authors.initials: S
authors.lastname: Roongjang
authors.forename: Kurena
authors.initials: K
authors.lastname: Takahashi
authors.forename: Myunji
authors.initials: M
authors.lastname: Park
authors.forename: Satoshi
authors.initials: S
authors.lastname: Obika
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imanishi
mesh_terms: D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051020:bcl-X Protein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D001952:Bridged-Ring Compounds; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D051020:bcl-X Protein