Amine modification reaction of C4'-oxidized abasic site generated from its caged precursors.
A method for the generation of ODNs containing a C4'-oxidized abasic site (C4'-OAS) from "photocaged" ODNs was investigated. We synthesized C4'-caged ODN 1 and C1'-caged ODN 2 bearing an o-nitrobenzyloxy group at the C-4' position and the C-1' position, respectively. The duplex stabilities of 1 and 2, with their complementary ODNs were studied by Tm measurements. The photochemical reaction of 1 and 2 to generate ODN 3 containing C4'-OAS, and the subsequent amine modification reaction were studied.
Usui
Kazuteru
K
Graduate School of pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Fukuda
Mitsuhiro
M
Aso
Mariko
M
Suemune
Hiroshi
H
D000588:Amines / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000528:radiation effects; D010084:Oxidation-Reduction; D013696:Temperature
D000588:Amines; D009838:Oligodeoxyribonucleotides; D004247:DNA