Synthesis and properties of double-stranded antisense oligonucleotides connected with a pentaerythritol linker.
This paper describes the synthesis and properties of double-stranded antisense oligonucleotides connected with a pentaerythritol linker. We found that double-stranded antisense oligonucleotides with aminomethyl residues have high affinity for single-stranded DNA or RNA in buffer solutions both with and without MgCl(2). Thus, these oligonucleotides would be useful as antisense oligonucleotides for targeting single-stranded RNA through triplex formation.
Shibata
Aya
A
Faculty of Engineering, Gifu University, Yanagido, Gifu 501-1193, Japan.
Ueno
Yoshihito
Y
Matsuda
Akira
A
Kitade
Yukio
Y
D004247:DNA / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D006860:Hydrogen Bonding; D009691:Nucleic Acid Denaturation; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D013696:Temperature
D004277:DNA, Single-Stranded; D016376:Oligonucleotides, Antisense; D011409:Propylene Glycols; C051802:triplex DNA; D012313:RNA; D004247:DNA; C008783:pentaerythritol