Use of pulsed-field gel electrophoresis of conserved XbaI fragments for identification of swine Salmonella serotypes.
Swine Salmonella isolates (n=674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with XbaI. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation of serotype to PFGE subtype. In addition, conserved fragments were identified within the restriction patterns that were unique to each serotype. PFGE using XbaI restriction provided a possible alternative method for screening and identifying swine Salmonella serotypes.
Gaul
Stephen B
SB
Department of Animal Science, Iowa State University, 11 Kildee Hall, Ames, IA 50011, USA.
Wedel
Stephanie
S
Erdman
Matthew M
MM
Harris
D L
DL
Harris
Isabel Turney
IT
Ferris
Kathleen E
KE
Hoffman
Lorraine
L
D000818:Animals; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D012475:Salmonella / Q000145:classification* / Q000235:genetics*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D004269:DNA, Bacterial; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific