title: Toxic effects of zearalenone and alpha-zearalenol on the regulation of steroidogenesis and testosterone production in mouse Leydig cells.
abstract: Zearalenone (ZEA) and its derivative alpha-zearalenol (alpha-ZOL) are produced by fungi of the genus Fusarium and, after ingestion via contaminated cereals, may lead to animal fertility disturbances and other reproductive pathologies. The previous study demonstrated the toxic effects of ZEA and alpha-ZOL through disturbances in male fertility and other reproductive pathologies in mice. In this study, we further examined the direct biological effects of ZEA and alpha-ZOL on steroidogenesis production, primarily in Leydig cells of mice. Mature mouse Leydig cells were purified by Percoll gradient centrifugation and the cell purity was determined by 3beta-hydroxysteroid dehydrogenase (3beta-HSD) staining. To examine ZEA and alpha-ZOL-induced biological consequences, we measured testosterone secretion and transcription level of 3 key steroidogenic enzymes including 3beta-HSD-1, P450scc and StAR, in ZEA and alpha-ZOL/human chorionicgonadotropin (hCG) co-treated cells. Our results showed that ZEA and alpha-ZOL (10(-4) M, 10(-6) M and 10(-8) M) significantly suppressed hCG (10 ng/ml)-induced testosterone secretion. The suppressive effect is correlated with a decrease in the level of transcription of 3beta-HSD-1, P450scc, and StAR (P<0.05).
authors.affiliation: Department of Animal Physiology, College of Biological Sciences, China Agricultural University, Beijing 100094, PR China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Yang
authors.forename: Yongfa
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Sheng
authors.initials: S
authors.lastname: Cui
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D017931:DNA Primers; D007985:Leydig Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013256:Steroids / Q000096:biosynthesis*; D013739:Testosterone / Q000096:biosynthesis*; D015025:Zearalenone / Q000633:toxicity*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000633:toxicity
chemical_list: D006063:Chorionic Gonadotropin; D017931:DNA Primers; D012333:RNA, Messenger; D013256:Steroids; C029659:zearalenol; D013739:Testosterone; D015025:Zearalenone; D015029:Zeranol; D015096:3-Hydroxysteroid Dehydrogenases