[Dynamics of mitochondrial ultrastructure in small pieces of cardiac tissue under long-term anoxic incubation].
Anoxic incubation of isolated small pieces of cardiac tissue for 72 h caused emergence of an unusual population of mitochondria, referred to as "mitochondrion inside mitochondrion". We studied dynamics of the origin of this event. In the most part of a mitochondrial population after a 6 h anoxic incubation of myocardial tissue, a local increase in some region of the intermembrane space was observed. Some regions of matrix with adjoined inner membrane move into these regions of intermembrane space, to be constricted eventually. After 12 h of incubation densely neighbouring layers of membrane are observed in these structures. By 24 h of incubation, inside new-formed structures well-distinguished concentric layers of membrane appear. Between these layers some electron-dense material ultrastructurally identical to mitochondrial matrix is seen. By 72 h of anoxic incubation, in cardiomyocytes of the experimental tissue structures with well-marked morphological features of mitochondria appear, which we called "mitochondrion inside mitochondrion". Results of our study are discussed in terms of a conception of changes that occur in the structure of mitochondrial reticulum during apoptosis.
Solodovnikova
I M
IM
Saprunova
V B
VB
Bakeeva
L E
LE
Iaguzhinskiĭ
L S
LS
D000818:Animals; D015687:Cell Hypoxia; D008929:Mitochondria, Heart / Q000648:ultrastructure*; D009132:Muscles / Q000503:physiopathology / Q000648:ultrastructure*; D009206:Myocardium / Q000648:ultrastructure*; D051381:Rats; D013997:Time Factors; D046509:Tissue Culture Techniques