title: Prognostic value of nuclear DNA quantification and cyclin A expression in epithelial ovarian carcinoma.
abstract: OBJECTIVE
  To investigate the correlation of DNA ploidy, S-phase fraction (SPF) and the expression of cyclin A to the clinical prognostic factors in patients with epithelial ovarian cancer.
  
  
  METHODS
  A prospective analysis was performed on 46 ovarian tumor patients. The DNA ploidy and SPF were determined by flow cytometry and the expression of cyclin A was measured by immunohistochemical staining.
  
  
  RESULTS
  Compared with benign and borderline tumors, the malignant tumors expressed higher levels of cyclin A (P=0.007), more aneuploid cells (P=0.018) and higher SPF (P=0.015). With regard to DNA ploidy and the clinical prognostic factors, aneuploid cells increased with tumor grade (P=0.011), the disease stage (P=0.009) and residual volume (P=0.001). When residual tumor was more than 2 cm, the expression of cyclin A was increased (P=0.043). Three-year survival was low for patients with tumors expressing cyclin A in over 10% of cells (P=0.003).
  
  
  CONCLUSIONS
  The data suggest that the assessment of the DNA ploidy, SPF and the expression of cyclin A may provide important information for predicting the prognosis of epithelial ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Women's Cancer Clinic, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Bo Sung
authors.initials: BS
authors.lastname: Yoon
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Kim
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Kim
authors.forename: Chong Seung
authors.initials: CS
authors.lastname: Lee
authors.forename: Jae Wook
authors.initials: JW
authors.lastname: Kim
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Sang Wun
authors.initials: SW
authors.lastname: Kim
authors.forename: Nam Hoon
authors.initials: NH
authors.lastname: Cho
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D019925:Cyclin A / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D005819:Genetic Markers; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality; D011003:Ploidies; D011379:Prognosis; D011446:Prospective Studies; D016196:S Phase; D015996:Survival Rate
chemical_list: D015415:Biomarkers; D019925:Cyclin A; D004273:DNA, Neoplasm; D005819:Genetic Markers