Single-pair FRET characterization of DNA tweezers.
The self-assembly properties of DNA make it an ideal choice for the construction of nanomachines such as DNA tweezers. Because nanomachines function as individual units, they need to be studied on the single-molecule level. From single-pair FRET investigations, we show that "open" tweezers exist in a single conformation with minimal FRET efficiency, whereas upon addition of a "closing strand", three conformations are observed, which are averaged out in ensemble measurements.
Müller
Barbara K
BK
Physical Chemistry, Department of Chemistry and Biochemistry, and Center for Nanoscience, Ludwig-Maximilians-Universität München, Butenandtstr. 11, Haus E, D-81377 Munich, Germany.
Reuter
Andreas
A
Simmel
Friedrich C
FC
Lamb
Don C
DC
D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D031541:Fluorescence Resonance Energy Transfer; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*
D009841:Oligonucleotides; D004247:DNA