Comparison study on PCR amplification of modified DNA by using various kinds of polymerase and modified nucleoside triphosphates.
A series of C5 substituted analogs of 2'-deoxyuridine triphosphate and 2'-deoxycytidine triphosphate were synthesized and investigated their substrate properties for thermostable DNA polymerases during polymerase chain reaction (PCR). The PCR assays indicated that the relative yield of the full-length product is dependent on whether the substitution is on dU or dC, and on the nature of the polymerase.
Kuwahara
Masayasu
M
Department of Applied Chemistry, Faculty of Engneering, Gunma University, 1-5-1 Tenjin-chou, Kiryu, Gunma 376-8515, Japan.
Tamura
Takehiro
T
Kitagata
Rina
R
Sawai
Hiroaki
H
Ozaki
Hiroaki
H
D004247:DNA / Q000096:biosynthesis / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003845:Deoxycytosine Nucleotides / Q000737:chemistry / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides / Q000737:chemistry / Q000378:metabolism*; D016133:Polymerase Chain Reaction
D003845:Deoxycytosine Nucleotides; D003856:Deoxyuracil Nucleotides; C027078:deoxyuridine triphosphate; C024107:2'-deoxycytidine 5'-triphosphate; D004247:DNA; D004259:DNA-Directed DNA Polymerase