title: [Construction of mutant myocilin plasmid and its expression in human trabecular meshwork cells].
abstract: OBJECTIVE
  To establish the mutant Myocilin gene plasmid in order to study the function of Myocilin.
  
  
  METHODS
  The mutant site was induced by site-directed mutagenesis. This plasmid were transfected into human trabecular meshwork cells (HTM cells) by cationic liposomes and the expression of Myocilin was examined by RT-PCR and Western blot analysis.
  
  
  RESULTS
  The mutant plasmid were correctly constructed. Myocilin was efficiently expressed in HTM cells transfected with this plasmid.
  
  
  CONCLUSIONS
  The constructed eukaryote expression plasmid pcDNA-MYOC-P370L could express Myocilin in HTM cells in vitro.
authors.affiliation: Key Laboratory of Ophthalmology of Ministry of Education and Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Ge
authors.forename: Bingqian
authors.initials: B
authors.lastname: Liu
authors.forename: Ge
authors.initials: G
authors.lastname: Song
authors.forename: Fei
authors.initials: F
authors.lastname: Hou
mesh_terms: D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D005902:Glaucoma, Open-Angle / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D017354:Point Mutation; D014129:Trabecular Meshwork / Q000166:cytology*; D014162:Transfection; D000099179:Myocilin
chemical_list: D003598:Cytoskeletal Proteins; D005136:Eye Proteins; D006023:Glycoproteins; D000099179:Myocilin