A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker.
Two isoforms of proliferating cell nuclear antigen (PCNA) have been observed in breast cancer cells. Commercially available antibodies to PCNA recognize both isoforms and, therefore, cannot differentiate between the PCNA isoforms in malignant and nonmalignant breast epithelial cells and tissues. We have developed a unique antibody that specifically detects a PCNA isoform (caPCNA) associated with breast cancer epithelial cells grown in culture and breast-tumor tissues. Immunostaining studies using this antibody suggest that the caPCNA isoform may be useful as a marker of breast cancer and that the caPCNA-specific antibody could potentially serve as a highly effective detector of malignancy. We also report here that the caPCNA isoform functions in breast cancer-cell DNA replication and interacts with DNA polymerase delta. Our studies indicate that the caPCNA isoform may be a previously uncharacterized detector of breast cancer.
Malkas
Linda H
LH
Division of Hematology and Oncology, Indiana University School of Medicine, and Indiana Cancer Center, Indianapolis, IN 46202, USA.
Herbert
Brittney Shea
BS
Abdel-Aziz
Waleed
W
Dobrolecki
Lacey E
LE
Liu
Yang
Y
Agarwal
Beamon
B
Hoelz
Derek
D
Badve
Sunil
S
Schnaper
Lauren
L
Arnold
Randy J
RJ
Mechref
Yehia
Y
Novotny
Milos V
MV
Loehrer
Patrick
P
Goulet
Robert J
RJ
Hickey
Robert J
RJ
D000906:Antibodies / Q000378:metabolism* / Q000502:physiology; D014408:Biomarkers, Tumor / Q000378:metabolism* / Q000502:physiology; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism*; D004258:DNA Polymerase III / Q000378:metabolism; D004261:DNA Replication / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism
D000906:Antibodies; D014408:Biomarkers, Tumor; D018809:Proliferating Cell Nuclear Antigen; D020033:Protein Isoforms; D004258:DNA Polymerase III