title: Proteomic analysis of mouse growth plate cartilage.
abstract: Cartilage is a highly specialized load-bearing tissue with a small number of cells and a high proportion of extracellular matrix (ECM). The abundance of heavily sulfated proteoglycans and a poorly soluble collagenous ECM presents a major technical challenge to 2-DE. Here we report proteomic analysis of mouse growth plate cartilage using novel methodology for tissue dissection and sample prefractionation. We have successfully resolved cartilage tissue extracts by 2-DE for the first time and identified cartilage ECM proteins by Western blotting and MS/MS.
authors.affiliation: Murdoch Childrens Research Institute and the Department of Paediatrics, University of Melbourne, Royal Children's Hospital, Parkville, Victoria, Australia.
authors.forename: Daniele
authors.initials: D
authors.lastname: Belluoccio
authors.forename: Richard
authors.initials: R
authors.lastname: Wilson
authors.forename: David J
authors.initials: DJ
authors.lastname: Thornton
authors.forename: Tristan P
authors.initials: TP
authors.lastname: Wallis
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Gorman
authors.forename: John F
authors.initials: JF
authors.lastname: Bateman
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D016326:Extracellular Matrix Proteins / Q000302:isolation & purification / Q000378:metabolism; D006132:Growth Plate / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D051379:Mice; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D016326:Extracellular Matrix Proteins; D020543:Proteome