Dystonin deficiency reduces taste buds and fungiform papillae in the anterior part of the tongue.
The anterior part of the tongue was examined in wild type and dystonia musculorum mice to assess the effect of dystonin loss on fungiform papillae. In the mutant mouse, the density of fungiform papillae and their taste buds was severely decreased when compared to wild type littermates (papilla, 67% reduction; taste bud, 77% reduction). The mutation also reduced the size of these papillae (17% reduction) and taste buds (29% reduction). In addition, immunohistochemical analysis demonstrated that the dystonin mutation reduced the number of PGP 9.5 and calbindin D28k-containing nerve fibers in fungiform papillae. These data together suggest that dystonin is required for the innervation and development of fungiform papillae and taste buds.
Ichikawa
H
H
Department of Oral Function and Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8525, Japan. hiroichi@md.okayama-u.ac.jp
Terayama
R
R
Yamaai
T
T
De Repentigny
Y
Y
Kothary
R
R
Sugimoto
T
T
D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D002352:Carrier Proteins / Q000235:genetics*; D002814:Chorda Tympani Nerve / Q000002:abnormalities / Q000378:metabolism / Q000503:physiopathology; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004195:Disease Models, Animal; D020821:Dystonic Disorders / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D000071821:Dystonin; D005830:Geniculate Ganglion / Q000002:abnormalities / Q000378:metabolism / Q000503:physiopathology; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000002:abnormalities / Q000378:metabolism / Q000503:physiopathology; D013650:Taste Buds / Q000002:abnormalities* / Q000378:metabolism* / Q000503:physiopathology; D013651:Taste Disorders / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D014059:Tongue / Q000002:abnormalities* / Q000378:metabolism* / Q000503:physiopathology; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
C576499:Calb1 protein, mouse; D064092:Calbindin 1; D064026:Calbindins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C095667:Dst protein, mouse; D000071821:Dystonin; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G; D043222:Ubiquitin Thiolesterase; C496976:Uchl1 protein, mouse