Electrochemical DNA hybridization detection using peptide nucleic acids and [Ru(NH3)6]3+ on gold electrodes.
An electrochemical DNA hybridization detection method based on the electrostatic interactions of [Ru(NH3)6]3+ cations with the anionic phosphate backbone of DNA is proposed. PNA molecules are immobilized as capture probes on the gold substrate. The cationic ruthenium complexes do not interact electrostatically with the PNA probes due to the absence of the anionic phosphate groups on the PNA probes. But after hybridization, [Ru(NH3)6]3+ is adsorbed on the DNA backbone, giving a clear hybridization detection signal in ac voltammetry. The analytical parameters (sensitivity, selectivity and reproducibility) are evaluated. Very good discrimination against the single-base mismatch A2143G, internal to the 23S rRNA gene of Helicobacter pylori, is observed. Moreover the system is successfully applied to the detection of complementary PCR amplicons.
Steichen
M
M
Service de Chimie Analytique et Chimie des Interfaces, Université Libre de Bruxelles, CP 255, Boulevard du Triomphe 2, B-1050 Bruxelles, Belgium.
Decrem
Y
Y
Godfroid
E
E
Buess-Herman
C
C
D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids; D017975:Ruthenium Compounds
D020135:Peptide Nucleic Acids; D017975:Ruthenium Compounds; D006046:Gold; D004247:DNA