[Adventitious root induction and in vitro culture of Panax notoginseng].
OBJECTIVE
To investigate the induction and culture of adventitious root of Panax notoginseng.


METHODS
Three ways, induction from the explants of three-year-old P. notoginseng. The explants of regenerated shoots and calluses, were used to induce adventitious roots. The effects of 2, 4-dichlorophenoxyacetic acid, indole-3-butyric acid and naphthylacetic acid on adventitious root induction were investigated respectively. The effects of four modes of separating adventitious roots from the parent tissues on culture in vitro were compared.


RESULTS
Adventitious roots were successfully induced by three methods, of which the young flower bud callus was the best material for the induction of adventitious root. Indole-3-butyric acid possessed the strongest potency for induction. The liquid culture system was established by continuous culture of adventitious roots together with their parent tissues before separated.


CONCLUSIONS
The acquisition and culture in vitro in liquid culture system of adventitious roots of P. notoginseng lay a foundation for the next investigation.
Gao
Xian-Fu
XF
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Xu
Zhao-Hui
ZH
Liu
Jia-Jian
JJ
Ma
Li-Ping
LP
Yin
Long-Ping
LP
Jia
Wei
W
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D053723:Panax notoginseng / Q000187:drug effects / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods*
D007211:Indoles; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; C014612:indolebutyric acid; D015084:2,4-Dichlorophenoxyacetic Acid; C034182:1-naphthaleneacetic acid