title: Effects of xapA and guaA disruption on inosine accumulation in Escherichia coli.
abstract: A xapA-disrupted mutant was studied to minimize hypoxanthine production and to improve inosine productivity in mutants of Escherichia coli. The xapA-disrupted mutant accumulated 5.6 g/l of inosine from 40 g/l of glucose, while the parent strain accumulated 4.6 g/l. This result indicates that xapA is activated in xapA-positive inosine-producers and that xapA disruption might be useful for improving inosine productivity.
authors.affiliation: Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.
authors.forename: Megumi
authors.initials: M
authors.lastname: Shimaoka
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takenaka
authors.forename: Yoko
authors.initials: Y
authors.lastname: Mihara
authors.forename: Osamu
authors.initials: O
authors.lastname: Kurahashi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsui
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D007288:Inosine / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D007288:Inosine