title: Biophysical and biochemical properties of oxanine-containing oligodeoxynucleotide.
abstract: Since oxanine (Oxa) was found as one of the major products from nitrosative oxidation of guanine, Oxa has been expected as mutagenic lesion related to NO-induced DNA damage. Here, to elucidate the mutagenic significance of Oxa in vitro, several Oxa-containing oligodeoxynucleotides (Oxa-ODNs) were synthesized and subjected to biophysical and biochemical characterization. Oxa-ODNs were analyzed in terms of their duplex stabilities and DNA polymerase responses. These results will be helpful in understanding the genotoxic pattern induced by Oxa.
authors.affiliation: International Innovation Center, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Seung Pil
authors.initials: SP
authors.lastname: Pack
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nonogawa
authors.forename: Nagendra Kumar
authors.initials: NK
authors.lastname: Kamisetty
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kodaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Makino
mesh_terms: D004256:DNA Polymerase I / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011684:Purine Nucleosides / Q000737:chemistry*; D013696:Temperature
chemical_list: D009838:Oligodeoxyribonucleotides; D011684:Purine Nucleosides; C468766:oxanine; D004256:DNA Polymerase I