Kinetics and thermodynamics of G-quadruplexes.
The melting of tetramolecular DNA or RNA quadruplexes is kinetically irreversible. However, rather than being a hindrance, this kinetic inertia allows us to study association and dissociation processes independently. General rules have been extended to longer DNA motifs or sequences containing modified bases such as 8-oxo or 7-deaza guanine. Results were compared with the canonical TG4T and TG5T tetramers: we demonstrate huge differences (up to 10(5)-fold) in the association constants of these quadruplexes depending on primary sequence.
Gros
Julien
J
Laboratoire de Biophysique, INSERM U565, CNRS UMR5153, Muséum National d'Histoire Naturelle, 43 rue Cuvier, 75005 Paris, France.
Webba da Silva
Mateus
M
De Cian
Anne
A
Amrane
Samir
S
Rosu
Frédéric
F
Bourdoncle
Anne
A
Saccà
Barbara
B
Alberti
Patrizia
P
Lacroix
Laurent
L
Mergny
Jean-Louis
JL
D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D006147:Guanine / Q000031:analogs & derivatives / Q000737:chemistry*; D007700:Kinetics; D013816:Thermodynamics
C024829:8-hydroxyguanine; D006147:Guanine; D004247:DNA; C066856:7-deazaguanine