title: Recombinant expression of Taenia solium TS14 antigen and its utilization for immunodiagnosis of neurocysticercosis.
abstract: In order to evaluate the potential use of TS14 antigen in an enzyme-linked immunosorbent assay (ELISA) for immunodiagnosis of neurocysticercosis (NC), its open reading frame (ORF) was amplified by RT-PCR from mRNA isolated from Taenia solium cysticerci. The ORF was subcloned into the expression vector pET-28a, and was used to transform Escherichia coli BL21 (DE3) cells to produce TS14 antigen. The His-tagged expressed protein was purified on a nickel affinity column. Using the HISTS14 as antigen, ELISA was positive for 100% of cerebrospinal fluid (CSF) and 97% of serum samples from NC patients. No positive results were observed with sera and CSF samples from control groups. Cross-reactivity with sera from patients with schistosomiasis and Chagas' disease was not observed. Serum samples from patients with taeniasis were evaluated and 2 of 13 cases showed reactivity in this assay. Our data indicate the usefulness of HISTS14 in ELISA for an accurate and rapid assay for diagnosis of NC and seroepidemiological studies.
authors.affiliation: Laboratório de Biologia Molecular, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, CEP 13565-905, SP, Brazil. marciamg@usp.br
authors.forename: Marcia Ramos Monteiro
authors.initials: MR
authors.lastname: da Silva
authors.forename: Antônio Augusto Mendes
authors.initials: AA
authors.lastname: Maia
authors.forename: Noeli Maria
authors.initials: NM
authors.lastname: Espíndola
authors.forename: Luís dos Ramos
authors.initials: Ldos R
authors.lastname: Machado
authors.forename: Adelaide José
authors.initials: AJ
authors.lastname: Vaz
authors.forename: Flávio
authors.initials: F
authors.lastname: Henrique-Silva
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism; D015801:Helminth Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D020019:Neurocysticercosis / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D013045:Species Specificity; D041201:Taenia solium / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins